From abright <@t> brightinstruments.com Mon Nov 1 05:20:10 2010 From: abright <@t> brightinstruments.com (Alan Bright) Date: Mon Nov 1 05:22:21 2010 Subject: [Histonet] cryostat question In-Reply-To: <8CD45EEBD9B9E32-EA4-3DF2@webmail-d038.sysops.aol.com> References: <8CD45EEBD9B9E32-EA4-3DF2@webmail-d038.sysops.aol.com> Message-ID: <925632415-1288606846-cardhu_decombobulator_blackberry.rim.net-905642111-@bda039.bisx.produk.on.blackberry> Dear Susan, Our Starlet 2212 Bench Top Cryostat has an option for orientation. There are many used for Mohs, as holding a low temperature is not a problem. I can email you some user references if. You want. Regards: Alan Bright Bright Instrument Co. Ltd. England Sent from my BlackBerry? wireless device -----Original Message----- From: historsd@aol.com Sender: histonet-bounces@lists.utsouthwestern.edu Date: Fri, 29 Oct 2010 22:10:21 To: Subject: [Histonet] cryostat question Does anyone have 'hands on' experience performing Mohs using a countertop cryostat? Can the specimen head be manipulated to acccomodate a badly embedded block? Does the machine stay cold enough to perform Mohs? Any advice would be appreciated. My e-mail is historsd@aol.com. Thanks, Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 01DoOb8La) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01DoOb8La&m=99b5dbb60f3d&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01DoOb8La&m=99b5dbb60f3d&c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01DoOb8La&m=99b5dbb60f3d&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS From adoughty <@t> lrri.org Mon Nov 1 07:47:50 2010 From: adoughty <@t> lrri.org (Doughty, Adele) Date: Mon Nov 1 07:48:01 2010 Subject: [Histonet] FW: I love this story Message-ID: Good morning sure was getting to talk to you the other day. This past weekend was the first time I heard dad complain that he did not feel good, headache and nausea. His hair is getting thinner. Not much else happening here thank goodness. Love ya, A ________________________________ "IT'S WHAT YOU SCATTER I was at the corner grocery store buying some early potatoes... I noticed a small boy, delicate of bone and feature, ragged but clean, hungrily apprising a basket of freshly picked green peas. I paid for my potatoes but was also drawn to the display of fresh green peas. I am a pushover for creamed peas and new potatoes. Pondering the peas, I couldn't help overhearing the conversation between Mr. Miller (the store owner) and the ragged boy next to me. 'Hello Barry, how are you today?' 'H'lo, Mr. Miller. Fine, thank ya. Jus' admirin' them peas. They sure look good.' 'They are good, Barry. How's your Ma?' 'Fine. Gittin' stronger alla' time.' 'Good. Anything I can help you with?' 'No, Sir. Jus' admirin' them peas.' Would you like to take some home?' Asked Mr. Miller. 'No, Sir. Got nuthin' to pay for 'em with.' 'Well, what have you to trade me for some of those peas?' 'All I got's my prize marble here.' 'Is that right? Let me see it' said Miller. 'Here 'tis. She's a dandy.' 'I can see that. Hmm mmm, only thing is this one is blue and I sort of go for red. Do you have a red one like this at home?' the store owner asked. 'Not zackley but almost.' 'Tell you what. Take this sack of peas home with you and next trip this way let me look at that red marble'. Mr. Miller told the boy. 'Sure will. Thanks Mr. Miller.' Mrs. Miller, who had been standing nearby, came over to help me. With a smile she said, 'There are two other boys like him in our community, all three are in very poor circumstances. Jim just loves to bargain with them for peas, apples, tomatoes, or whatever. When they come back with their red marbles, and they always do, he decides he doesn't like red after all and he sends them home with a bag of produce for a green marble or an orange one, when they come on their next trip to the store.' I left the store smiling to myself, impressed with this man. A short time later I moved to Colorado , but I never forgot the story of this man, the boys, and their bartering for marbles. Several years went by, each more rapid than the previous one. Just recently I had occasion to visit some old friends in that Idaho community and while I was there learned that Mr. Miller had died. They were having his visitation that evening and knowing my friends wanted to go, I agreed to accompany them. Upon arrival at the mortuary we fell into line to meet the relatives of the deceased and to offer whatever words of comfort we could. Ahead of us in line were three young men. One was in an army uniform and the other two wore nice haircuts, dark suits and white shirts...all very professional looking. They approached Mrs. Miller, standing composed and smiling by her husband's casket. Each of the young men hugged her, kissed her on the cheek, spoke briefly with her and moved on to the casket. Her misty light blue eyes followed them as, one by one; each young man stopped briefly and placed his own warm hand over the cold pale hand in the casket. Each left the mortuary awkwardly, wiping his eyes. Our turn came to meet Mrs. Miller. I told her who I was and reminded her of the story from those many years ago and what she had told me about her husband's bartering for marbles. With her eyes glistening, she took my hand and led me to the casket. 'Those three young men who just left were the boys I told you about. They just told me how they appreciated the things Jim 'traded' them. Now, at last, when Jim could not change his mind about color or size....they came to pay their debt.' 'We've never had a great deal of the wealth of this world,' she confided, 'but right now, Jim would consider himself the richest man in Idaho ..' With loving gentleness she lifted the lifeless fingers of her deceased husband. Resting underneath were three exquisitely shined red marbles. The Moral: We will not be remembered by our words, but by our kind deeds. Life is not measured by the breaths we take, but by the moments that take our breath. Today I wish you a day of ordinary miracles ~ A fresh pot of coffee you didn't make yourself... An unexpected phone call from an old friend.... Green stoplights on your way to work.... The fastest line at the grocery store.... A good sing-along song on the radio... Your keys found right where you left them. Send this to the people you'll never forget. I just did... If you don't send it to anyone, it means you are in way too much of a hurry to even notice the ordinary miracles when they occur. IT'S NOT WHAT YOU GATHER, BUT WHAT YOU SCATTER THAT TELLS WHAT KIND OF LIFE YOU HAVE LIVED! "Life is too short for drama and petty things, laugh insanely, love truly and forgive quickly." From raestask <@t> grics.net Mon Nov 1 08:25:18 2010 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Mon Nov 1 08:25:25 2010 Subject: [Histonet] Pax 8 Message-ID: <20101101092518.lgj9zz1ts1y8k8go@webmail2.centurytel.net> Does anyone know where to purchase Pax 8 antibody for formalin fixed paraffin embedded tissue? Rae Staskiewicz Methodist Medical Center From flnails <@t> texaschildrens.org Mon Nov 1 08:58:47 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon Nov 1 08:59:02 2010 Subject: [Histonet] RE: Medite Microtomes In-Reply-To: <731941C266951A47BEF11E5EFAAED9C90655967D@nsmvexch01.northside.local> References: <731941C266951A47BEF11E5EFAAED9C90655967D@nsmvexch01.northside.local> Message-ID: I have had some experience with this company and their embedding station but not microtome. The small cold area where you orient the tissue tends to ice over. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Fields Sent: Saturday, October 30, 2010 4:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Medite Microtomes Hi Netters, Does anyone out there use the Medite Microtomes? If so, have you had any problems with them? I purchased 3 of them less than a year ago and I have already sent them back to Germany because of advancing (back and forth) issues. They are telling me (of course) no one else has had these issues and they want to send me a third set of them (the 2nd set are demos that are having some of the same issues). I would appreciate any feed back on their microtomes and company. Thank you in advance....I'm afraid I have made a big mistake in buying these. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From MDiCarlo <@t> KaleidaHealth.Org Mon Nov 1 09:18:49 2010 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Mon Nov 1 09:18:58 2010 Subject: [Histonet] aluminum gutter guard Message-ID: <1B73766A27A1554CB2729B6432E813010259290C@KALEXMB04.KaleidaHealth.org> Hello Histonetters, I have been using aluminum gutter guard to wrap my bones when I manually process them- from increasing percentages of alcohols to xylene and paraffin. I am running very low on it and I have not been able to find it at any hardware stores. I looked on line and Amazon is out of stock and doesn't even know if they will still carry it. Does anyone know what I can use to replace it? I appreciate your help. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From sfeher <@t> CMC-NH.ORG Mon Nov 1 10:51:27 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Nov 1 10:51:36 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 46 In-Reply-To: <8593425C-2AC9-4DE2-A3FD-6DBD73EDFEE1@gmail.com> References: <4ccc4f42.4420ec0a.3409.1973SMTPIN_ADDED@mx.google.com> <8593425C-2AC9-4DE2-A3FD-6DBD73EDFEE1@gmail.com> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC2426C81A@exchange.cmc-nh.org> http://www.cytopathnet.org/tiki-index.php Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miranda D. Felton Sent: Saturday, October 30, 2010 1:43 PM To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 46 Does anyone know of a cytology listserv? Thank you Miranda Sent from my iPod On Oct 30, 2010, at 1:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: lymph nodes peeling out of OCT (Monfils, Paul) > 2. PLP fixative (Truscott, Tom) > 3. Precipitating Silver (Sheila Haas) > 4. RE: Precipitating Silver (Monfils, Paul) > 5. RE: PLP fixative (WILLIAM DESALVO) > 6. IHC Staining Problem (Jennifer Hill) > 7. Laura Miller is Out of the Office. > (Laura.Miller@leica-microsystems.com) > 8. RE: RE: breast fixation times (Patsy Ruegg) > 9. cryostat question (historsd@aol.com) 10. Re: IHC Staining > Problem (Jennifer MacDonald) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 29 Oct 2010 13:13:44 -0400 > From: "Monfils, Paul" > Subject: RE: [Histonet] lymph nodes peeling out of OCT > To: > Message-ID: > > <4EBFF65383B74D49995298C4976D1D5E075E08F9@LSRIEXCH1.lsmaster.lifespan. > org > > > > Content-Type: text/plain; charset="us-ascii" > > I place all tissues in liquid OCT for at least 15 minutes before > freezing. This virtually eliminates the problem of tissue sections > separating from the OCT after sectioning. > > > > > > ------------------------------ > > Message: 2 > Date: Fri, 29 Oct 2010 10:55:45 -0700 > From: "Truscott, Tom" > Subject: [Histonet] PLP fixative > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <44F1D6D7EB8CC84F92859EE5C4E6ECB4011B20F1B562@CVMMBX.vetmed.wsu.edu> > Content-Type: text/plain; charset="us-ascii" > > I am seeking basic information on PLP fixative. What is it? Can I make > it or buy it? How long does fixation take for lymph node? > Thanks in advance, Tom Truscott > > > ------------------------------ > > Message: 3 > Date: Fri, 29 Oct 2010 10:56:14 -0700 (PDT) > From: Sheila Haas > Subject: [Histonet] Precipitating Silver > To: histonet@lists.utsouthwestern.edu > Message-ID: <450265.9811.qm@web57802.mail.re3.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Has anyone heard of precipitating silver out of a solution by adding > table salt? > I read this somewhere as a means of disposing of silver easily. Any > info would be appreciated. > Thanks, > > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc. > > > > > ------------------------------ > > Message: 4 > Date: Fri, 29 Oct 2010 14:02:26 -0400 > From: "Monfils, Paul" > Subject: RE: [Histonet] Precipitating Silver > To: > Message-ID: > > <4EBFF65383B74D49995298C4976D1D5E075E090C@LSRIEXCH1.lsmaster.lifespan. > org > > > > Content-Type: text/plain; charset="iso-8859-1" > > Yes, it is easy to do. Since silver chloride is insoluble in water, > chloride added to a solution containing ionic silver will cause the > white compound to precipitate out immediately. It will settle out > onto the bottom of the container overnight, or can be filtered out. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Friday, October 29, 2010 1:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Precipitating Silver > > Has anyone heard of precipitating silver out of a solution by adding > table salt? > I read this somewhere as a means of disposing of silver easily. Any > info would be appreciated. > Thanks, > > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Fri, 29 Oct 2010 12:52:06 -0600 > From: WILLIAM DESALVO > Subject: RE: [Histonet] PLP fixative > To: , histonet > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Follow the links for a receipe. > > www.pathology.ufl.edu/~molecular/PLP%20Fixative.doc > > http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno > /reagents.cfm > > www.hopkinsmedicine.org/.../multimedia/text_documents/Recipes_For_Maki > ng_PLP_Fixative.doc > > > William DeSalvo, B.S., HTL(ASCP) > > > > > >> From: ttruscot@vetmed.wsu.edu >> To: histonet@lists.utsouthwestern.edu >> Date: Fri, 29 Oct 2010 10:55:45 -0700 >> Subject: [Histonet] PLP fixative >> >> I am seeking basic information on PLP fixative. What is it? Can I >> make it or buy it? How long does fixation take for lymph node? >> Thanks in advance, Tom Truscott >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 6 > Date: Fri, 29 Oct 2010 20:05:13 +0000 > From: Jennifer Hill > Subject: [Histonet] IHC Staining Problem > To: histonet > Message-ID: > > <8AA2173DC209CA438077A832FF98BD7F02806442@VETMXHT.ads.vet.k-state.edu > > > Content-Type: text/plain; charset="iso-8859-1" > > I hope someone can help me with a problem I've been having with my > CD79a hand stain. This is a stain that was validated and working > perfectly, when a few months ago I started having reduced, to no > staining on my controls, with the occasional run staining a little > stronger. I followed the discussion not too long ago with the person > who was having similar problems, but the suggestions didn't seem to > apply (I use Tween 20 in my PBS rinses, and we use APEX slides from > Surgipath-not Fischer Plus). I've tried everything I can think of and > I hope someone may have some other ideas. For reference this is for a > veterinary diagnostic lab, not a human lab, and this is the only stain > we've been having this issue with. > > My protocol is as follows: After deparfinization/rehydration, AR for > 20 min in the steamer in Biogenex Citra Solution (I've gotten new > Citra Solution, tried a pressure cooker) > 5 min peroxide quench, followed by a 10 min protein block using Dako's > Protein Block-Serum Free 60 min Primary incubation (Dako CD79a) at 37 > C (decreased dilution from 1:100 to 1:65, new bottle of antibody) 30 > min incubation at room temp of ImmPRESS anti-Mouse Reagent from Vector > Develop stain with DAB chromogen from Vector > > Washes of PBS/Tween 20 follow between steps > > Thank you, > Jennifer Hill > Research Assistant > Kansas State University > Veterinary Diagnostic Lab > > > > ------------------------------ > > Message: 7 > Date: Fri, 29 Oct 2010 17:34:50 -0500 > From: Laura.Miller@leica-microsystems.com > Subject: [Histonet] Laura Miller is Out of the Office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > systems.com > > > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 10/29/2010 and will not return > until 11/01/2010. > > I am out of the office for the rest of the day. I will be back on > Monday! > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > > > > ------------------------------ > > Message: 8 > Date: Fri, 29 Oct 2010 16:38:02 -0600 > From: "Patsy Ruegg" > Subject: RE: [Histonet] RE: breast fixation times > To: "'Kuhnla, Melissa'" , "'Feher, > Stephen'" > , "'Tench, Bill'" , > "'Weems, > Joyce'" , > Message-ID: <0AD2F85778424E90B81B4CA2D469CDDC@prueggihctechlt> > Content-Type: text/plain; charset="us-ascii" > > Melissa, > > Just because the clone you are using is not recommended in the > guidelines does not mean that you cannot use it. You have to validate > it the same as using a clone they do recommend, but the bottom line is > you can use what ever you want in what ever way you want as long as > you validate the protocol on samples generated in your lab. If you > want to use a fixation or processing different from what is > recommended you have to compare it to formalin fixation and "standard" > (whatever that is) paraffin processing, you would have to do a side by > side comparison on the same tissue. As long as you follow the > fixation and processing guidelines you can use the ab u want to use > without comparing it to abs they recommend, as far as I can tell. > You must validate that ab on at least 25 samples generated in your > institution just as you would have to validate an antibody they do > recommend. > > Regards, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Kuhnla, Melissa > Sent: Friday, October 29, 2010 3:51 AM > To: Feher, Stephen; Tench, Bill; Weems, Joyce; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: breast fixation times > > One more question regarding ER/PR/Her2..who am I kidding, we will be > talking about them forever!! With regards to the ER/PR publication: > The > PR clone (Ventana 1E2) that we currently use is not listed as > 'acceptable'. Is anyone else in this situation? Are you switching to > another clone?? Have you come across anything speaking of this > scenario? > What to do if you currently run a clone not mentioned? It will be a > relatively easy validation for me, but I can't image I am alone here. > Thanks, Melissa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, > Stephen > Sent: Thursday, October 28, 2010 5:50 PM > To: Tench, Bill; Weems, Joyce; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: breast fixation times > > Great discussion, comprehensive yet concise. Thanks Bill and Joyce > and Melissa. > > > Steve > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, > Bill > Sent: Wednesday, October 27, 2010 12:26 PM > To: Weems, Joyce; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: breast fixation times > > My apologies for not including the updates accurate for ER and PR. > > > Bill Tench > Associate Dir. Laboratory Services > Chief, Cytology Services > Palomar Medical Center > 555 E. Valley Parkway > Escondido, California 92025 > Bill.Tench@pph.org > Voice: 760- 739-3037 > Fax: 760-739-2604 > > -----Original Message----- > From: Weems, Joyce [mailto:JWeems@sjha.org] > Sent: Wednesday, October 27, 2010 9:22 AM > To: Tench, Bill; histonet@lists.utsouthwestern.edu > Subject: RE: breast fixation times > > Thanks for this good explanation, Bill. > > One can not follow the guidelines and document the variant in the > report, but not following them could hurt the patient if there is a > clinical trial they might participate in. Clinical trials follow the > protocol to the letter and if the FDA requirement is not met, the > patient can not participate. > > The times were extended for ER and PR to 72 hours, but NOT yet for > Her2. > So...because the tissue is all the same, we must follow the 48 hour > limit. We just had a case this weekend. Had the clinical staff remove > it from the processor on Sun morning and embedded it Monday. We don't > ususally have this problem as we are a 6-day lab, but it was finished > too late on Fri. > > Cheers,j > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, > Bill > Sent: Wednesday, October 27, 2010 11:50 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] breast fixation times > > There is no exception for core biopsies, as reasonable as that may > seem. > I have had that discussion with the purveyors of the guidelines. 6-48 > is the current standard. there was a lot of discussion about > exceeding > 48 and using the FISH option. My colleague responsible for this > wrote: > It is in the CAP checklist, ANP 22998: > > If the laboratory assesses Her2 by IHC or Her2 gene amplification by > in-situ hybridization (FISH, CISH, SISH), does the lab have a > documented procedure for ensuring appropriate length of fixation of > specimens tested? > > Specimens subject to Her2 testing should be fixed in 10% neutral > buffered formalin for at least 6 hours and no longer than 48 hours. > While fixation outside of these time limits is not an absolute > exclusion criterion for Her2 testing, labs should qualify any negative > results for specimens fixed less than 6 hours or longer than 48 hours. > For cases with negative results by IHC, consideration should be given > to performing confirmatory analysis by in-situ hybridization. > > There is also a table in the original ASCO/CAP Guideline > Recommendations for Her2 in Breast Cancer (Arch Pathol Lab Med, Vol > 131, Jan 2007) that states that tissue fixed in formalin for greater > than > 48 > hours is not an absolute exclusion criterion, but if known to be fixed > longer than 48 hours or unknown, the report should qualify any > negative result with this information (table 6). > > As for upcoming changes, i don't know other than these time > limitations are suppose to be more rigorously applied to ER and PR, > along with the newly instituted documentation of time between excision > and time placed in fixative. > > > Bill Tench > Associate Dir. Laboratory Services > Chief, Cytology Services > Palomar Medical Center > 555 E. Valley Parkway > Escondido, California 92025 > Bill.Tench@pph.org > Voice: 760- 739-3037 > Fax: 760-739-2604 > > > [None] made the following annotations > --------------------------------------------------------------------- > Confidential E-Mail: This e-mail is intended only for the person or > entity to which it is addressed, and may contain information that is > privileged, confidential, or otherwise protected from disclosure. > Dissemination, distribution, or copying of this e-mail or the > information herein by anyone other than the intended recipient is > prohibited. If you have received this e-mail in error, please notify > the sender by reply e-mail, and destroy the original message and all > copies. > > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If > you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > > [None] made the following annotations > --------------------------------------------------------------------- > Confidential E-Mail: This e-mail is intended only for the person or > entity to which it is addressed, and may contain information that is > privileged, confidential, or otherwise protected from disclosure. > Dissemination, distribution, or copying of this e-mail or the > information herein by anyone other than the intended recipient is > prohibited. If you have received this e-mail in error, please notify > the sender by reply e-mail, and destroy the original message and all > copies. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail, and any attachments therein, is > confidential and for use by the intended addressee only. If this > message is received by you in error please do not disseminate or read > further. Please reply to the sender that you have received the message > in error, then delete the message. > Although Catholic Health Services of Long Island attempts to sweep e- > mail and attachments for viruses, it does not guarantee that either > are virus-free and accepts no liability for any damage sustained as a > result of viruses. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 9 > Date: Fri, 29 Oct 2010 22:10:21 -0400 > From: historsd@aol.com > Subject: [Histonet] cryostat question > To: histonet@lists.utsouthwestern.edu > Message-ID: <8CD45EEBD9B9E32-EA4-3DF2@webmail-d038.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > Does anyone have 'hands on' experience performing Mohs using a > countertop cryostat? Can the specimen head be manipulated to > acccomodate a badly embedded block? Does the machine stay cold enough > to perform Mohs? Any advice would be appreciated. > My e-mail is historsd@aol.com. Thanks, Susan > > > ------------------------------ > > Message: 10 > Date: Fri, 29 Oct 2010 22:44:58 -0700 > From: Jennifer MacDonald > Subject: Re: [Histonet] IHC Staining Problem > To: Jennifer Hill > Cc: histonet , > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > > > Content-Type: text/plain; charset="US-ASCII" > > How far in advance are your controls being cut? Are your patient > tissues staining? > > > > > Jennifer Hill > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/29/2010 01:11 PM > > To > histonet > cc > > Subject > [Histonet] IHC Staining Problem > > > > > > > I hope someone can help me with a problem I've been having with my > CD79a hand stain. This is a stain that was validated and working > perfectly, when a few months ago I started having reduced, to no > staining on my controls, with the occasional run staining a little > stronger. I followed the discussion not too long ago with the person > who was having similar problems, but the suggestions didn't seem to > apply (I use Tween 20 in my PBS rinses, and we use APEX slides from > Surgipath-not Fischer Plus). I've tried everything I can think of and > I hope someone may have some other ideas. For reference this is for a > veterinary diagnostic lab, not a human lab, and this is the only stain > we've been having this issue with. > > My protocol is as follows: After deparfinization/rehydration, AR for > 20 min in the steamer in Biogenex Citra Solution (I've gotten new > Citra Solution, tried a pressure cooker) > 5 min peroxide quench, followed by a 10 min protein block using Dako's > Protein Block-Serum Free 60 min Primary incubation (Dako CD79a) at 37 > C (decreased dilution from 1:100 to 1:65, new bottle of antibody) 30 > min incubation at room temp of ImmPRESS anti-Mouse Reagent from Vector > Develop stain with DAB chromogen from Vector > > Washes of PBS/Tween 20 follow between steps > > Thank you, > Jennifer Hill > Research Assistant > Kansas State University > Veterinary Diagnostic Lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 83, Issue 46 > **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Mon Nov 1 11:15:34 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Nov 1 11:15:44 2010 Subject: [Histonet] Nashville TN Ht Position Message-ID: <6F33D8418806044682A391273399860F05CA5448@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Nov 1 11:20:23 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Nov 1 11:21:05 2010 Subject: [Histonet] Pax 8 In-Reply-To: <20101101092518.lgj9zz1ts1y8k8go@webmail2.centurytel.net> References: <20101101092518.lgj9zz1ts1y8k8go@webmail2.centurytel.net> Message-ID: Biocare, They have a prediluted. Works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz [raestask@grics.net] Sent: Monday, November 01, 2010 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pax 8 Does anyone know where to purchase Pax 8 antibody for formalin fixed paraffin embedded tissue? Rae Staskiewicz Methodist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dreynold <@t> mdanderson.org Mon Nov 1 11:23:14 2010 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Mon Nov 1 11:23:19 2010 Subject: [Histonet] IHC Staining Problem In-Reply-To: <64f156ad-c1be-4072-aa16-2408e4ef2a40@DCPWPRTR02.mdanderson.edu> References: <64f156ad-c1be-4072-aa16-2408e4ef2a40@DCPWPRTR02.mdanderson.edu> Message-ID: <785BBF0C5F49CE41BA74460A43A08F022B67DD6953@DCPWVMBXC0VS3.mdanderson.edu> > >We had a similar problem a few years back. We had used an antibody at 1:500 dilution for years. Got in a new bottle and no labeling. Called the manufacture and they assured me it was the same concentration. Finally went to 1:50 dilution and the staining was like our old stain. Over the last few years with each new vial I titer and we are now up to 1:200 dilution. So I suspect it is your antibody. When the problem began I still had enough of the old vial to run them simultaneously and the old vial was working fine. > ------------------------------ > > Message: 10 > Date: Fri, 29 Oct 2010 22:44:58 -0700 > From: Jennifer MacDonald > Subject: Re: [Histonet] IHC Staining Problem > To: Jennifer Hill > Cc: histonet , > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset="US-ASCII" > > How far in advance are your controls being cut? Are your patient > tissues > staining? > > > > > Jennifer Hill > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/29/2010 01:11 PM > > To > histonet > cc > > Subject > [Histonet] IHC Staining Problem > > > > > > > I hope someone can help me with a problem I've been having with my > CD79a > hand stain. This is a stain that was validated and working perfectly, > when a few months ago I started having reduced, to no staining on my > controls, with the occasional run staining a little stronger. I > followed > the discussion not too long ago with the person who was having similar > problems, but the suggestions didn't seem to apply (I use Tween 20 > in my > PBS rinses, and we use APEX slides from Surgipath-not Fischer > Plus). I've > tried everything I can think of and I hope someone may have some other > ideas. For reference this is for a veterinary diagnostic lab, not a > human > lab, and this is the only stain we've been having this issue with. > > My protocol is as follows: After deparfinization/rehydration, > AR for 20 min in the steamer in Biogenex Citra Solution (I've gotten > new > Citra Solution, tried a pressure cooker) > 5 min peroxide quench, followed by a 10 min protein block using Dako's > Protein Block-Serum Free > 60 min Primary incubation (Dako CD79a) at 37 C (decreased dilution > from > 1:100 to 1:65, new bottle of antibody) > 30 min incubation at room temp of ImmPRESS anti-Mouse Reagent from > Vector > Develop stain with DAB chromogen from Vector > > Washes of PBS/Tween 20 follow between steps > > Thank you, > Jennifer Hill > Research Assistant > Kansas State University > Veterinary Diagnostic Lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 83, Issue 46 > **************************************** ------------------------------ ****** From FUNKM <@t> mercyhealth.com Mon Nov 1 12:03:51 2010 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Mon Nov 1 12:04:08 2010 Subject: [Histonet] Standard work Message-ID: <4CCEACA7.9B87.00AC.0@mercyhealth.com> Hist We are looking at tracking system and would like some additional data from labs that are tracking standard work for sectioning, embedding, labeling. Also special stains and IHC would be helpful. Like to compare our lab with other labs to see if we are in the ballpark. Appreciate any help you might share. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 From Janice.Mahoney <@t> alegent.org Mon Nov 1 12:13:47 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Mon Nov 1 12:14:22 2010 Subject: [Histonet] Standard work In-Reply-To: <4CCEACA7.9B87.00AC.0@mercyhealth.com> References: <4CCEACA7.9B87.00AC.0@mercyhealth.com> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354E13@EXCHMBC2.ad.ah.local> Hi Marcia, I'd be glad to help but could you be more specific as to what you need? We have the Ventana Vangage system and love it. We are a Lean Lab as I know you are and I think this system fits in perfectly. Please feel free to contact me for specifics. We have Job guidance sheets (i.e. procedures) that follow step by step how to use the system during each of the stations for Vantage. Everyone does it the same way every time and we have only had 2 numbering errors in 2 years with Vantage. Standard work and single piece flow are essential. We post the flow at each station and do audits to assure compliance. Everyone loves the system and feels much less stress. >From the Vantage data I am able to collect I know my average cutting time per block is just over 2 minutes and embedding is less than a minute. Let me know what additional info you need. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Monday, November 01, 2010 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Standard work Hist We are looking at tracking system and would like some additional data from labs that are tracking standard work for sectioning, embedding, labeling. Also special stains and IHC would be helpful. Like to compare our lab with other labs to see if we are in the ballpark. Appreciate any help you might share. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Melissa.Kuhnla <@t> chsli.org Mon Nov 1 12:38:47 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Mon Nov 1 12:39:41 2010 Subject: [Histonet] Standard work In-Reply-To: <4CCEACA7.9B87.00AC.0@mercyhealth.com> References: <4CCEACA7.9B87.00AC.0@mercyhealth.com> Message-ID: Hi Marcia, We are a core lab...doing the work of four hospitals. We have been live with Vantage (Ventana) for about nine months. We have 3 symphonies, 3 ultras, 1 XT, and two nexus special stainers all tied into the system. I would be happy to answer specifics for you. We are happy. Ventana seems good about working with us as far as current and future needs that the system can not meet at this time. In particular with regards to shipping of specimens, cassettes and slides from one facility to another. Let me know if you have questions. Melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Monday, November 01, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Standard work Hist We are looking at tracking system and would like some additional data from labs that are tracking standard work for sectioning, embedding, labeling. Also special stains and IHC would be helpful. Like to compare our lab with other labs to see if we are in the ballpark. Appreciate any help you might share. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From Allison_Scott <@t> hchd.tmc.edu Mon Nov 1 13:54:35 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Mon Nov 1 13:54:48 2010 Subject: [Histonet] Frozen Section TAT Message-ID: <1872B4A455B7974391609AD8034C79FC026DFD00@LBEXCH01.hchd.local> Hello to all in histoland. How is everyone calculating the FS TAT. Does any anyone have a file that automatically calculates that tat when you plug in the data, that they would be willing to share. Any help would be appreciated. Allison Scott Histology Supervisor LBJ Hospital. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From trathborne <@t> somerset-healthcare.com Mon Nov 1 14:19:30 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Nov 1 14:20:12 2010 Subject: [Histonet] Frozen Section TAT In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD00@LBEXCH01.hchd.local> Message-ID: We have recently started using our LIS to help with this. When the specimen is accessioned (in Cerner Millennium) as a frozen section, tasks are generated. Additional tasks can be added depending on how many slides or additional blocks are prepared for the FS. These tasks can then be verified when complete (i.e. when the pathologist calls the results to the OR). All of this is heavily relied upon by the person entering the tasks accurately and in a timely manner. We have only started this process this week, so it will be interesting watching the trends. Reports can then be generated monthly and by pathologist. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scott, Allison D Sent: Monday, November 01, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Section TAT Hello to all in histoland. How is everyone calculating the FS TAT. Does any anyone have a file that automatically calculates that tat when you plug in the data, that they would be willing to share. Any help would be appreciated. Allison Scott Histology Supervisor LBJ Hospital. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From JWeems <@t> sjha.org Mon Nov 1 14:23:43 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Nov 1 14:23:50 2010 Subject: [Histonet] RE: Frozen Section TAT In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD00@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFD00@LBEXCH01.hchd.local> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F073@CHEXCMS10.one.ads.che.org> Our pathologist dictate the times and we review it manually for 100% of the frozens!! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, November 01, 2010 14:55 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Section TAT Hello to all in histoland. How is everyone calculating the FS TAT. Does any anyone have a file that automatically calculates that tat when you plug in the data, that they would be willing to share. Any help would be appreciated. Allison Scott Histology Supervisor LBJ Hospital. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From victor <@t> pathology.washington.edu Mon Nov 1 14:23:10 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Nov 1 14:24:27 2010 Subject: [Histonet] Frozen Section TAT In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD00@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFD00@LBEXCH01.hchd.local> Message-ID: <4CCF139E.8090807@pathology.washington.edu> Allison, We have 2 fields in the database for when the frozen specimen was received and when the surgeon was notified. We can then run a report to show us the cases with frozens and the TAT. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 11/1/2010 11:54 AM, Scott, Allison D wrote: > Hello to all in histoland. How is everyone calculating the FS TAT. > Does any anyone have a file that automatically calculates that tat when > you plug in the data, that they would be willing to share. Any help > would be appreciated. > > Allison Scott > Histology Supervisor > LBJ Hospital. > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Mon Nov 1 15:34:27 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Nov 1 15:34:30 2010 Subject: [Histonet] IHC Staining Problem Message-ID: Hi, I think you mentioned using citrate retrieval. Have you tried high pH retrieval with EDTA or an equivalent? I think CD79 works better with high pH retrieval. Amos Message: 1 Date: Sun, 31 Oct 2010 19:29:59 +0000 From: Jennifer Hill Subject: RE: [Histonet] IHC Staining Problem To: histonet Message-ID: <8AA2173DC209CA438077A832FF98BD7F02806542@VETMXHT.ads.vet.k-state.edu > Content-Type: text/plain; charset="iso-8859-1" Thanks to everyone who has responded so far. To answer the questions asked, our controls are either being cut the same day or the day or two before, so they are not setting for extended periods. We have also used at least three or four different controls, all with the same results. (The controls stain fine for other tests: CD20, CD3, etc.)The pathologists are saying that the patient slides are not staining as well. So it doesn't seem to be something so simple as a control issue. As to the dehydration/deparafinization, that takes place on an automated stainer, and no other stains are having issues so I don't believe that is the issue either. Jennifer Hill Kansas State University Veterinary Diagnostic Lab From ross <@t> premierlab.com Mon Nov 1 16:00:53 2010 From: ross <@t> premierlab.com (Ross Benik) Date: Mon Nov 1 15:59:01 2010 Subject: [Histonet] IHC Staining Problem In-Reply-To: Message-ID: We have developed a staining protocol for the CD79a antibody from two different vendors and both require a HIER TRIS/EDTA pH 9.0 retrieval. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Monday, November 01, 2010 2:34 PM To: jhill@vet.k-state.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Staining Problem Hi, I think you mentioned using citrate retrieval. Have you tried high pH retrieval with EDTA or an equivalent? I think CD79 works better with high pH retrieval. Amos Message: 1 Date: Sun, 31 Oct 2010 19:29:59 +0000 From: Jennifer Hill Subject: RE: [Histonet] IHC Staining Problem To: histonet Message-ID: <8AA2173DC209CA438077A832FF98BD7F02806542@VETMXHT.ads.vet.k-state.edu > Content-Type: text/plain; charset="iso-8859-1" Thanks to everyone who has responded so far. To answer the questions asked, our controls are either being cut the same day or the day or two before, so they are not setting for extended periods. We have also used at least three or four different controls, all with the same results. (The controls stain fine for other tests: CD20, CD3, etc.)The pathologists are saying that the patient slides are not staining as well. So it doesn't seem to be something so simple as a control issue. As to the dehydration/deparafinization, that takes place on an automated stainer, and no other stains are having issues so I don't believe that is the issue either. Jennifer Hill Kansas State University Veterinary Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Nov 1 15:42:30 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 1 16:09:20 2010 Subject: [Histonet] Frozen Section TAT In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD00@LBEXCH01.hchd.local> Message-ID: <319005.96176.qm@web65706.mail.ac4.yahoo.com> Under separate cover I am sending something on this subject. Ren? J. --- On Mon, 11/1/10, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Frozen Section TAT To: histonet@lists.utsouthwestern.edu Date: Monday, November 1, 2010, 2:54 PM Hello to all in histoland.? How is everyone calculating the FS TAT. Does any anyone have a file that automatically calculates that tat when you plug in the data, that they would be willing to share.? Any help would be appreciated.? Allison Scott Histology Supervisor LBJ Hospital. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kblack <@t> digestivehlth.com Mon Nov 1 16:32:50 2010 From: kblack <@t> digestivehlth.com (Konni Black) Date: Mon Nov 1 16:32:58 2010 Subject: [Histonet] HT/HTL Opening Tualatin, Oregon Message-ID: <447B7F5DD73941498B3276004362A345@digestivehlth.com> An experienced HT or HTL is needed for a new histology lab in Tualatin, Oregon. Great company, very flexible schedule, excellent pay. Please contact Konni Black if you are interested. kblack@digestivehlth.com or 253-503-2560. From histotech <@t> imagesbyhopper.com Mon Nov 1 16:48:46 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Nov 1 16:49:20 2010 Subject: [Histonet] Frozen Section TAT In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD00@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFD00@LBEXCH01.hchd.local> Message-ID: <822729E3-7DB1-4594-8242-870CC7A360C3@imagesbyhopper.com> Allison, I have created an excel spreadsheet where you put time started, time completed & it will calculate the amount time spent doing the frozen. It highlights any time grater than 20 min. Let me know if you are interested in something like that. Michelle On Nov 1, 2010, at 2:54 PM, "Scott, Allison D" wrote: > Hello to all in histoland. How is everyone calculating the FS TAT. > Does any anyone have a file that automatically calculates that tat when > you plug in the data, that they would be willing to share. Any help > would be appreciated. > > Allison Scott > Histology Supervisor > LBJ Hospital. > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lbustamante <@t> cvm.tamu.edu Mon Nov 1 17:41:30 2010 From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante) Date: Mon Nov 1 17:42:22 2010 Subject: [Histonet] THANK YOU !!!! Message-ID: <4CCEFBCA020000B9000E4697@CVM.TAMU.EDU> To all of you.... for the answer to my questions concerning 100 um thick plastic sections and Absolute v/s absolute+methanol. Thanks again.... Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 From BSullivan <@t> shorememorial.org Tue Nov 2 06:14:52 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Tue Nov 2 06:17:51 2010 Subject: [Histonet] Frozen Section TAT In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD00@LBEXCH01.hchd.local> Message-ID: We have a spread sheet where ALL times are written. We put the time received in the lab, the time the tech places the specimen in the cryostat, the time the case is taken to the Pathologist and the time the Pathologist signs the case out. At the end of the month I look through all of the sheets and calculate the time for each case. It has to be completed within 20 minutes. If it is not I look for reasons and state these in my end of the month report. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Scott, Allison D" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Frozen Section TAT 11/01/2010 02:54 PM Hello to all in histoland. How is everyone calculating the FS TAT. Does any anyone have a file that automatically calculates that tat when you plug in the data, that they would be willing to share. Any help would be appreciated. Allison Scott Histology Supervisor LBJ Hospital. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anna.Inman <@t> stmarygj.org Tue Nov 2 06:28:30 2010 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Tue Nov 2 06:28:36 2010 Subject: [Histonet] validation of tissue processor Message-ID: <2925AE271EAAD440AF48FCCEB8002D091443E50A@smgmail01.smgj.sclhs.net> Hello Histoland 1. I am just curious what process is followed for validating a new tissue processor? 2. Do you validate every IHC stain again or can you simply re-validate a representative sample of IHC and be ok? Thank you as always for your wealth of knowledge Anna SMH Pathology Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mhale <@t> carisls.com Tue Nov 2 09:21:03 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Tue Nov 2 09:21:08 2010 Subject: [Histonet] Nashville HT Message-ID: <6F33D8418806044682A391273399860F05D1B573@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From susanbachus <@t> verizon.net Tue Nov 2 10:22:37 2010 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Tue Nov 2 10:22:40 2010 Subject: [Histonet] Can anyone put an old Mettler microbalance to good use? In-Reply-To: References: Message-ID: I have an old surplus Mettler balance that is no longer worth recalibrating--I hate to waste anything & am wondering if anyone might find it useful enough for a display or something like that to be worth the cost of shipping? From MW <@t> PersonifySearch.com Tue Nov 2 13:58:39 2010 From: MW <@t> PersonifySearch.com (Matthew Ward) Date: Tue Nov 2 13:58:45 2010 Subject: [Histonet] Field Support Specialist Opportunity with a World Leader in MD/VA/DC Message-ID: <01df01cb7abf$f6532f20$e2f98d60$@com> Good Afternoon Everyone, Our team her at Personify is currently partnered with a World Leader in Histology and IHC, and we currently are searching for Histotechnologists with experience on IHC equipment for a Field Support Role in MD, VA, and DC. This position offers great compensation and benefits and offers career growth as the company is expanding rapidly. Please contact me directly to learn more. Thanks! Matt Ward Account Executive Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com Field Support Specialist The Company: Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. The Opportunity: The company currently has an opening for a Field Support Specialist in Clinical and Molecular Diagnostics to cover MD, VA and Washington DC. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Base: $60-65k per year + bonus/commission Target Compensation: $72k Other: Full benefits - 401k program/matching Primary Responsibilities: The primary responsibility of this role will be to provide in-field technical applications support for current and next generation range of products. The FSS will install/set-up instrumentation in customer laboratories, perform demonstrations and maintain demonstration equipment in a clean and operational manner. The FSS will also train customers on the use of instrumentation. Conducting in-field post purchase applications support and performing instrument validations will be a key responsibility of this position. Additional Responsibilities: - Build an in-depth understanding of new product technologies and their applications in a diagnostic environment - Contribute to the strategic planning process by providing technical data on instrumentation and reagents - Conduct post-purchase reagent order management - Be the customer liaison by providing scientific and laboratory expertise for in-field installations, evaluations, training and trouble-shooting - Provide customer support for remote problem solving - Design and perform experiments to investigate and solve tough technical applications problems Education and Experience Required: BA/BS in Life Sciences or equivalent. 1 to 3 years Histology laboratory experience in clinical, research or industrial setting with a practical focus on IHC. 1-3 years experience in the optimization, operation, functionality and support of both manual and automated IHC instruments and related products is highly desired but not a requirement. Candidate must be self motivated, goal oriented and results driven with excellent interpersonal and communication skills. From Rick.Garnhart <@t> memorialhealthsystem.com Tue Nov 2 15:27:49 2010 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Tue Nov 2 15:27:54 2010 Subject: [Histonet] Tracking Systems In-Reply-To: Message-ID: Does the Vantana system use bar-coding? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From rsrichmond <@t> gmail.com Tue Nov 2 16:46:30 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Nov 2 16:46:33 2010 Subject: [Histonet] Re: Frozen Section TAT Message-ID: Some comments on this dismal piece of busy work: It helps to have a time stamper so the person receiving the specimen can stamp the time on the requisition. A work sheet for each frozen section case makes it easier for the pathologist to record the time the report is telephoned, the frozen section diagnosis, and other useful information. A clock by the frozen section microscope is both a reminder and a hint, particularly for those of us who don't wear wrist watches in the lab. Turning a single block case around in 20 minutes isn't very difficult. The usual cause of delay is a difficult diagnosis where more than one pathologist looks at the slides. There is no TAT requirement for multiple block cases. Pathologist compliance is a major issue. Some pathologists entirely refuse to record TAT. Frozen sections are the highest stress area of most pathologists' practice, and it's easy to forget procedural details, particularly this one, which is of no benefit to the patient, nor to anyone else except the paper-pushers. Bob Richmond Samurai Pathologist Knoxville TN From histotech <@t> imagesbyhopper.com Tue Nov 2 17:52:13 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Nov 2 17:52:53 2010 Subject: [Histonet] Re: Frozen Section TAT In-Reply-To: References: Message-ID: We have a calendar book in the frozen section room. The pathologist places the patient label on the book on the appropriate date, writes both the start & stop times. I don't count the multiple block ones, but I don't tell them that! ;o) If they stay in the habit, all the better for me! Oh yeah, we have a large clock on the wall, easy to find, see and read! Michelle On Nov 2, 2010, at 5:46 PM, Robert Richmond wrote: > Some comments on this dismal piece of busy work: > > It helps to have a time stamper so the person receiving the specimen > can stamp the time on the requisition. > > A work sheet for each frozen section case makes it easier for the > pathologist to record the time the report is telephoned, the frozen > section diagnosis, and other useful information. > > A clock by the frozen section microscope is both a reminder and a > hint, particularly for those of us who don't wear wrist watches in the > lab. > > Turning a single block case around in 20 minutes isn't very difficult. > The usual cause of delay is a difficult diagnosis where more than one > pathologist looks at the slides. > > There is no TAT requirement for multiple block cases. > > Pathologist compliance is a major issue. Some pathologists entirely > refuse to record TAT. Frozen sections are the highest stress area of > most pathologists' practice, and it's easy to forget procedural > details, particularly this one, which is of no benefit to the patient, > nor to anyone else except the paper-pushers. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From suetp918 <@t> comcast.net Tue Nov 2 19:08:16 2010 From: suetp918 <@t> comcast.net (Sue) Date: Tue Nov 2 19:08:18 2010 Subject: [Histonet] Frozen Section TAT In-Reply-To: Message-ID: <614553536.843124.1288742896586.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> I like Beatrice's method but I think it would only work in a small setting where you are not getting multiple frozens at one time as well as frozens that have 30 or 40 parts or working with multiple pathologists. Susan T. Paturzo TJUH From BSullivan <@t> shorememorial.org Wed Nov 3 06:15:42 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Wed Nov 3 06:18:42 2010 Subject: [Histonet] Tracking Systems In-Reply-To: Message-ID: Yes. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Rick.Garnhart@mem orialhealthsystem .com To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Tracking Systems 11/02/2010 04:27 PM Does the Vantana system use bar-coding? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> shorememorial.org Wed Nov 3 06:20:45 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Wed Nov 3 06:23:40 2010 Subject: [Histonet] Frozen Section TAT In-Reply-To: <614553536.843124.1288742896586.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: Sue, Yes you do have your hands full. We also do multiple parts on skins, nodes, etc. etc. It is stated on the QC sheet and as part of our procedure that it is based on the initial frozen. If there is an outlier because of multiple parts that is stated as the justification for the lapse in procedure. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Sue To BSullivan@shorememorial.org 11/02/2010 08:08 cc PM histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthweste rn.edu, Allison D Scott Subject Re: [Histonet] Frozen Section TAT I like Beatrice's method but I think it would only work in a small setting where you are not getting multiple frozens at one time as well as frozens that have 30 or 40 parts or working with multiple pathologists. Susan T. Paturzo TJUH From Janice.Mahoney <@t> alegent.org Wed Nov 3 08:21:59 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Nov 3 08:23:34 2010 Subject: [Histonet] Tracking Systems In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354E30@EXCHMBC2.ad.ah.local> Yes, at your accessioning, grossing, embedding, cutting, sendout(slide distribution, and archiving (filing)areas. It has so much more in terms of reporting and the data you can generate from putting a date and time stamp, and a person's name at every point in your process. It also allows you to communicate from one bench to the next with special instructions and to log quality issues at each of your benches. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rick.Garnhart@memorialhealthsystem.com Sent: Tuesday, November 02, 2010 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking Systems Does the Vantana system use bar-coding? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From alyssa <@t> alliedsearchpartners.com Wed Nov 3 10:23:16 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Nov 3 10:23:22 2010 Subject: [Histonet] Histotech Needed in New Jersey Message-ID: Allied Search Partners is currently accepting resumes for a histotechnician/histotechnologist. Ideally we are looking for a candidate with at least 3 years experience. Location: Clifton, NJ area *Essential Functions and Duties* Must be able to work independently under minimal supervision, maintain laboratory supplies, equipment, and QC/QA records Must be knowledgeable with basic computer skills and pathology software, and participate in improvement of laboratory procedures. The person should be reliable with great inter-personal communication skills, and willing to coordinate with other departmental staff. *Shift:* Day Shift Hours *Requirements* Bachelor?s degree preferred but not required HT (ASCP) preferred The Bachelor degree may be waived if the candidate has extensive experience (>5 years) At least 3 years of histotechnology experience including routine histology, cytology preparation, immunohistochemistry staining (automated), special stain (manual, for bone marrow and others). Flow Cytometry experience is a plus To apply for this position please submit resume to alyssa@alliedsearchpartners.com for initial prescreening. No resume will be submitted to client until we speak to you for a phone interview. All resumes kept confidential. -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From cstaak <@t> personifysearch.com Wed Nov 3 10:45:07 2010 From: cstaak <@t> personifysearch.com (Corey Staak) Date: Wed Nov 3 10:45:14 2010 Subject: [Histonet] Great Opportunity in Chicago with a Top-Tier Manufacturer of Core Histology and IHC Message-ID: <004d01cb7b6e$18099010$481cb030$@com> Job Opportunity in the Chicago Area!! Application Specialist/ Histology Trainer working with a World Leader in Cancer Diagnostics. Great Base Salary and Gold Standard Benefits!! Please Contact me directly. Thanks! Regards, Corey Staak Executive Recruiter Laboratory Practice Personify 5020 Weston Parkway suite 315 Cary, North Carolina 27513 (Tel) 800.875.6188 direct ext 112 (Fax) 919.460.8726 www.personifysearch.com Talent Management | Outsourced Recruitment From lcates <@t> synecor.com Wed Nov 3 12:23:21 2010 From: lcates <@t> synecor.com (Lynne Cates) Date: Wed Nov 3 12:23:25 2010 Subject: [Histonet] Staining infarct size by TTC method Message-ID: I am looking for a procedure or a protocol for staining infarct size in rabbit hearts using the TTC method. I have tried several methods but I am not satisfied with end results. Would appreciate any help. Thanks in advance Lynne Cates, HT (ASCP) Synecor, LLC 3908 Patriot Drive Suite# 170 Durham, NC 27703 Phone 919-541-9977 x 119 lcates@synecor.com From Sharon.Davis-Devine <@t> carle.com Wed Nov 3 14:48:33 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Nov 3 14:48:37 2010 Subject: [Histonet] Milestone RHS Microwave Processor Message-ID: We have a Milestone RHS Microwave Processor for sale because we do not use it anymore. It is 3 years old and in excellent shape. Please contact Kevin Schnieder at 754-551-5913 or email him at kevin.schneider@MEDmarketplace.com if interested. We need to get rid of it soon so we can use the counterspace it is sitting on. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From med_leni <@t> yahoo.com Wed Nov 3 15:21:56 2010 From: med_leni <@t> yahoo.com (Pop Elena) Date: Wed Nov 3 15:22:00 2010 Subject: [Histonet] unstained paraffin tissue slides storage Message-ID: <581490.96363.qm@web30901.mail.mud.yahoo.com> Hello, I found here a few disscussions regarding the storage of tissue slides but I did not find a clear answer to the questions I have. I would really appreciate an answer from?anybody that has experience with this. I need to store for long term a bunch?of unstained tissue slides for the purpose of doing immunostaining even in a few years from now on. Unfortunatelly they were stored for about 3 years at room temperature. What?it is usually?recomended: to store them at -20 degrees Celsius? If yes, is it OK to store them in the regular 100 slides boxes? And when you need to start an immunostaining just take them out of the freezer and let them at room temp for a while before starting the stain or what procedure do you use? I heard some?labs keep them in nitrogen gas containers. Do you have any info about this? Any imput is appreciated! Thanks! From sgoebel <@t> xbiotech.com Wed Nov 3 15:26:25 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Nov 3 15:26:29 2010 Subject: [Histonet] unstained paraffin tissue slides storage Message-ID: <20101103132625.9e2d9aa830e8449a2412eb1e4f2f067e.610a5f6e98.wbe@email04.secureserver.net> Plain slide boxes is ok. I think you can store them for up to a year in a fridg. (4 degrees), but I usually pu them in a freezer (-20). Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] unstained paraffin tissue slides storage From: Pop Elena Date: Wed, November 03, 2010 1:21 pm To: histonet@lists.utsouthwestern.edu Hello, I found here a few disscussions regarding the storage of tissue slides but I did not find a clear answer to the questions I have. I would really appreciate an answer from anybody that has experience with this. I need to store for long term a bunch of unstained tissue slides for the purpose of doing immunostaining even in a few years from now on. Unfortunatelly they were stored for about 3 years at room temperature. What it is usually recomended: to store them at -20 degrees Celsius? If yes, is it OK to store them in the regular 100 slides boxes? And when you need to start an immunostaining just take them out of the freezer and let them at room temp for a while before starting the stain or what procedure do you use? I heard some labs keep them in nitrogen gas containers. Do you have any info about this? Any imput is appreciated! Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Nov 3 15:51:32 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Nov 3 15:51:39 2010 Subject: [Histonet] unstained paraffin tissue slides storage In-Reply-To: <581490.96363.qm@web30901.mail.mud.yahoo.com> References: <581490.96363.qm@web30901.mail.mud.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB51A0@PHSXMB30.partners.org> We always stored our unstained paraffin slides in slides boxes at -80 degrees. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pop Elena Sent: Wednesday, November 03, 2010 4:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] unstained paraffin tissue slides storage Hello, I found here a few disscussions regarding the storage of tissue slides but I did not find a clear answer to the questions I have. I would really appreciate an answer from?anybody that has experience with this. I need to store for long term a bunch?of unstained tissue slides for the purpose of doing immunostaining even in a few years from now on. Unfortunatelly they were stored for about 3 years at room temperature. What?it is usually?recomended: to store them at -20 degrees Celsius? If yes, is it OK to store them in the regular 100 slides boxes? And when you need to start an immunostaining just take them out of the freezer and let them at room temp for a while before starting the stain or what procedure do you use? I heard some?labs keep them in nitrogen gas containers. Do you have any info about this? Any imput is appreciated! Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From hfedor <@t> jhmi.edu Wed Nov 3 15:54:55 2010 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Wed Nov 3 15:55:02 2010 Subject: [Histonet] unstained paraffin tissue slides storage In-Reply-To: <20101103132625.9e2d9aa830e8449a2412eb1e4f2f067e.610a5f6e98.wbe@email04.secureserver.net> References: <20101103132625.9e2d9aa830e8449a2412eb1e4f2f067e.610a5f6e98.wbe@email04.secureserver.net> Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D317825B472A@JHEMTEXVS3.win.ad.jhu.edu> Hello, We have been storing our slides in very small Ziploc bags at -20 and find that this method works fairly well. We have done a study(Berez et.al in process) and slides stored in this fashion for 5 years stain better than freshly cut slides from the same blocks that have been stored at room temperature. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St,?| Marburg Room 406 Baltimore, MD?| 21287-7065 410.614.1660 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Wednesday, November 03, 2010 4:26 PM To: Pop Elena Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage Plain slide boxes is ok. I think you can store them for up to a year in a fridg. (4 degrees), but I usually pu them in a freezer (-20). Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] unstained paraffin tissue slides storage From: Pop Elena Date: Wed, November 03, 2010 1:21 pm To: histonet@lists.utsouthwestern.edu Hello, I found here a few disscussions regarding the storage of tissue slides but I did not find a clear answer to the questions I have. I would really appreciate an answer from anybody that has experience with this. I need to store for long term a bunch of unstained tissue slides for the purpose of doing immunostaining even in a few years from now on. Unfortunatelly they were stored for about 3 years at room temperature. What it is usually recomended: to store them at -20 degrees Celsius? If yes, is it OK to store them in the regular 100 slides boxes? And when you need to start an immunostaining just take them out of the freezer and let them at room temp for a while before starting the stain or what procedure do you use? I heard some labs keep them in nitrogen gas containers. Do you have any info about this? Any imput is appreciated! Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Thu Nov 4 04:41:01 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Nov 4 04:41:39 2010 Subject: [Histonet] unstained paraffin tissue slides storage In-Reply-To: <3201CF51728F6048A24FA3AFFFEEF1D317825B472A@JHEMTEXVS3.win.ad.jhu.edu> References: <20101103132625.9e2d9aa830e8449a2412eb1e4f2f067e.610a5f6e98.wbe@email04.secureserver.net> <3201CF51728F6048A24FA3AFFFEEF1D317825B472A@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: <7722595275A4DD4FA225B92CDBF174A1FDA0AEB7BC@EXC-MBX3.cfs.le.ac.uk> Room temperature storage has always worked fine for us, of course it all depends on the quality of fixation and processing, and what antigen you are after.......... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: 03 November 2010 20:55 To: sgoebel@xbiotech.com; Pop Elena Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage Hello, We have been storing our slides in very small Ziploc bags at -20 and find that this method works fairly well. We have done a study(Berez et.al in process) and slides stored in this fashion for 5 years stain better than freshly cut slides from the same blocks that have been stored at room temperature. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St,?| Marburg Room 406 Baltimore, MD?| 21287-7065 410.614.1660 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Wednesday, November 03, 2010 4:26 PM To: Pop Elena Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage Plain slide boxes is ok. I think you can store them for up to a year in a fridg. (4 degrees), but I usually pu them in a freezer (-20). Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] unstained paraffin tissue slides storage From: Pop Elena Date: Wed, November 03, 2010 1:21 pm To: histonet@lists.utsouthwestern.edu Hello, I found here a few disscussions regarding the storage of tissue slides but I did not find a clear answer to the questions I have. I would really appreciate an answer from anybody that has experience with this. I need to store for long term a bunch of unstained tissue slides for the purpose of doing immunostaining even in a few years from now on. Unfortunatelly they were stored for about 3 years at room temperature. What it is usually recomended: to store them at -20 degrees Celsius? If yes, is it OK to store them in the regular 100 slides boxes? And when you need to start an immunostaining just take them out of the freezer and let them at room temp for a while before starting the stain or what procedure do you use? I heard some labs keep them in nitrogen gas containers. Do you have any info about this? Any imput is appreciated! Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Kuhnla <@t> chsli.org Thu Nov 4 08:16:22 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Thu Nov 4 08:16:29 2010 Subject: [Histonet] eosin as tissue dye Message-ID: I know using eosin as a tissue dye during processing has been a recent discussion. My lab has been experiencing issue with the cobalt blue we use currently. At embedding, small biopsies are not seen on blue sponges, white sponges turn blue. Biopsies that are wrapped are not turning blue, the paper is not allowing the blue thru. Eosin has come up as a suggestion. I know it is known to fluoresce. What is everyone's experience with this?? The only FISH test we are running is Her2 by pathvysion. What recipe are you all using?? On the processor?? Thank you so much. Melissa The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From talulahgosh <@t> gmail.com Thu Nov 4 09:07:45 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Nov 4 09:07:48 2010 Subject: [Histonet] unstained paraffin tissue slides storage--why cold? Message-ID: Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx From LSebree <@t> uwhealth.org Thu Nov 4 09:40:05 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Nov 4 09:40:11 2010 Subject: [Histonet] unstained paraffin tissue slides storage--why cold? In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E273839A088@UWHC-MAIL01.uwhis.hosp.wisc.edu> Some antigens degrade and lose antigenicity in paraffin sections when stored at RT. Storage at below freezing temps slows this process down . Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, November 04, 2010 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Thu Nov 4 10:21:03 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Thu Nov 4 10:21:08 2010 Subject: [Histonet] unstained paraffin tissue slides storage--why =?UTF-8?Q?cold=3F?= Message-ID: <20101104082103.9e2d9aa830e8449a2412eb1e4f2f067e.6830c31a46.wbe@email04.secureserver.net> -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay "viable". I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Nov 4 10:48:21 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 4 10:48:28 2010 Subject: [Histonet] unstained paraffin tissue slides storage--why cold? In-Reply-To: <20101104082103.9e2d9aa830e8449a2412eb1e4f2f067e.6830c31a46.wbe@email04.secureserver.net> Message-ID: <994824.74932.qm@web65710.mail.ac4.yahoo.com> The blocks also degrade but just on the surface, where the air oxygen can act. When you use the block for recuts to be used in IHC you always have to resurface the block, hence eliminating the oxidized surface layer and exposing the preserved deeper layer that you are actually using in the test. Ren? J. --- On Thu, 11/4/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? To: "Emily Sours" Cc: histonet@lists.utsouthwestern.edu Date: Thursday, November 4, 2010, 11:21 AM -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20).? I think the main point of doing this from what I understand is so that the antigens stay "viable".? I know over time they can degrade and so your stain won't work with some antibodies.? The weirdest part to me has always been that you don't have to store the blocks this way.? So I think that was your question, if the blocks aren't stored in a freezer why store the slides?? Won't the antigens in the blocks start to degrade as well?? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas? 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Nov 4 10:48:34 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Nov 4 10:50:14 2010 Subject: [Histonet] RE: eosin as tissue dye In-Reply-To: References: Message-ID: We have not had any problem with fluorescence with our her 2 FISH or any complaints from the Cytogenetics labs which run many other types of FISH. We use eosin in our processors. The eosin will bleed out when you deparaffinize the slides. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa [Melissa.Kuhnla@chsli.org] Sent: Thursday, November 04, 2010 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eosin as tissue dye I know using eosin as a tissue dye during processing has been a recent discussion. My lab has been experiencing issue with the cobalt blue we use currently. At embedding, small biopsies are not seen on blue sponges, white sponges turn blue. Biopsies that are wrapped are not turning blue, the paper is not allowing the blue thru. Eosin has come up as a suggestion. I know it is known to fluoresce. What is everyone's experience with this?? The only FISH test we are running is Her2 by pathvysion. What recipe are you all using?? On the processor?? Thank you so much. Melissa The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Thu Nov 4 10:56:15 2010 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Nov 4 10:56:21 2010 Subject: [Histonet] unstained paraffin tissue slides storage--why cold? In-Reply-To: <20101104082103.9e2d9aa830e8449a2412eb1e4f2f067e.6830c31a46.wbe@email04.secureserver.net> References: <20101104082103.9e2d9aa830e8449a2412eb1e4f2f067e.6830c31a46.wbe@email04.secureserver.net> Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D3178268356B@JHEMTEXVS3.win.ad.jhu.edu> The study that we did showed that the staining on freshly cut slides from a block stored at room temperature was in fact not as good as slides that were sectioned 5 years earlier and stored at -20. Therefore the blocks should be stored in the cold as well. The tissue is degrading in the block and on the slides and the cold does slow down the process. The fixation in lots of the tissue is not optimal. Fixing for 48 hours will definitely be better than the current fixation practices going on in most clinical labs, if what you want is the best tissue preservation possible. But most of us do not have that option. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Thursday, November 04, 2010 11:21 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay "viable". I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Nov 4 11:09:16 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Nov 4 11:09:31 2010 Subject: [Histonet] unstained paraffin tissue slides storage--why cold? In-Reply-To: <3201CF51728F6048A24FA3AFFFEEF1D3178268356B@JHEMTEXVS3.win.ad.jhu.edu> References: <20101104082103.9e2d9aa830e8449a2412eb1e4f2f067e.6830c31a46.wbe@email04.secureserver.net> <3201CF51728F6048A24FA3AFFFEEF1D3178268356B@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: Helen, did you write a paper from that study? Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, November 04, 2010 8:56 AM To: sgoebel@xbiotech.com; Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? The study that we did showed that the staining on freshly cut slides from a block stored at room temperature was in fact not as good as slides that were sectioned 5 years earlier and stored at -20. Therefore the blocks should be stored in the cold as well. The tissue is degrading in the block and on the slides and the cold does slow down the process. The fixation in lots of the tissue is not optimal. Fixing for 48 hours will definitely be better than the current fixation practices going on in most clinical labs, if what you want is the best tissue preservation possible. But most of us do not have that option. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Thursday, November 04, 2010 11:21 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay "viable". I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu Nov 4 11:14:09 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Nov 4 11:14:15 2010 Subject: [Histonet] Qualifications to run a Thin Prep 2000? Message-ID: Dear Histonetters, I am interested in someone from the Cytyc Corp or a Cytology lab supervisor contacting me regarding the educational/training requirements for personnel running a Thin Prep 2000 instrument. I am on assignment at a Histology lab in Illinois where they process their own cytology specimens and they have a position open for someone to accession specimens and run the Thin Prep 2000. The Laboratory Director is of the opinion that they need to hire an HT or HTL to run this instrument, and I'm not so sure. I know I've worked in labs where they had "lab assistants" (college kids @ $8./hr) preparing thin preps. If the respondent could cite the relevant CAP regulation regarding the Thin Prep instrument, it would be fantastic. Thank you so much. Lee Luna Lives! Jay A. Lundgren M.S., HTL (ASCP) From JWeems <@t> sjha.org Thu Nov 4 11:29:57 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Nov 4 11:30:01 2010 Subject: [Histonet] Qualifications to run a Thin Prep 2000? In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F65A@CHEXCMS10.one.ads.che.org> Please tell me lab assistants can run the ThinPrep. I've had one doing it for years! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Thursday, November 04, 2010 12:14 To: histonet Subject: [Histonet] Qualifications to run a Thin Prep 2000? Dear Histonetters, I am interested in someone from the Cytyc Corp or a Cytology lab supervisor contacting me regarding the educational/training requirements for personnel running a Thin Prep 2000 instrument. I am on assignment at a Histology lab in Illinois where they process their own cytology specimens and they have a position open for someone to accession specimens and run the Thin Prep 2000. The Laboratory Director is of the opinion that they need to hire an HT or HTL to run this instrument, and I'm not so sure. I know I've worked in labs where they had "lab assistants" (college kids @ $8./hr) preparing thin preps. If the respondent could cite the relevant CAP regulation regarding the Thin Prep instrument, it would be fantastic. Thank you so much. Lee Luna Lives! Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From TGoins <@t> mt.gov Thu Nov 4 11:41:45 2010 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Nov 4 11:41:51 2010 Subject: [Histonet] unstained paraffin tissue slides storage--why cold? In-Reply-To: <20101104082103.9e2d9aa830e8449a2412eb1e4f2f067e.6830c31a46.wbe@email04.secureserver.net> References: <20101104082103.9e2d9aa830e8449a2412eb1e4f2f067e.6830c31a46.wbe@email04.secureserver.net> Message-ID: For storage of multiple IHC control blocks - we store at room temperature but cut from only one in the series at a time. After the tissue is verified as a good positive control, the cut surface of the block is "painted over" with melted paraffin from the embedding station to prevent exposure to the air. Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Thursday, November 04, 2010 9:21 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay "viable". I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu Nov 4 11:42:13 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Nov 4 11:42:17 2010 Subject: [Histonet] Qualifications to run a Thin Prep 2000? In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F65A@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F65A@CHEXCMS10.one.ads.che.org> Message-ID: Personally, I think a trained monkey could run the thing, provided an ample supply of bananas. From cdubord <@t> umphysicians.umn.edu Thu Nov 4 11:45:36 2010 From: cdubord <@t> umphysicians.umn.edu (Claudia DuBord) Date: Thu Nov 4 11:45:57 2010 Subject: [Histonet] Tzank procedure, hair testing Message-ID: <4CD29CE0020000800005D817@ump-gwia.umphysicians.com> We have a derm provider who wants to start billing out CPT 88160 or 88161 and also 96902. I am looking on information about what work the provider actually has to do and what documentation is required. If anyone could help, I would appreciate it. Claudia DuBord, B.A., CPC, CPC-I Senior Education Analyst/Certified Coding Instructor University of Minnesota Physicians 612-884-0811 **CONFIDENTIALITY NOTICE** DO NOT READ THIS EMAIL IF YOU ARE NOT THE INTENDED RECIPIENT. The information in this email may contain confidential and/or privileged material. If you are not the intended recipient, your review, forwarding, copying, distribution, or any other use or disclosure of any information in this email is prohibited. If you received this email in error, please destroy and delete this message from any computer and contact us immediately by return email. From shive003 <@t> umn.edu Thu Nov 4 11:47:37 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Nov 4 11:47:41 2010 Subject: [Histonet] unstained paraffin tissue slides storage--why cold? References: <20101104082103.9e2d9aa830e8449a2412eb1e4f2f067e.6830c31a46.wbe@email04.secureserver.net> Message-ID: <8E86B65DC4054E47A8730F0DEB9EDCE0@auxs.umn.edu> We keep all of our control blocks and slides at 4C in plastic boxes, and date all unstained slides with date of sectioning. Jan Shivers UMN Vet Diag Lab St. Paul, MN ----- Original Message ----- From: "Goins, Tresa" To: ; "Emily Sours" Cc: Sent: Thursday, November 04, 2010 11:41 AM Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? > For storage of multiple IHC control blocks - we store at room temperature > but cut from only one in the series at a time. After the tissue is > verified as a good positive control, the cut surface of the block is > "painted over" with melted paraffin from the embedding station to prevent > exposure to the air. > > > Tresa Goins > Veterinary Diagnostic Lab > Department of Livestock > Bozeman, Montana > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > sgoebel@xbiotech.com > Sent: Thursday, November 04, 2010 9:21 AM > To: Emily Sours > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] unstained paraffin tissue slides storage--why > cold? > > -70 or -80 seems a little extreme to me, that's why I always just leave > them in a normal freezer (-20). I think the main point of doing this > from what I understand is so that the antigens stay "viable". I know > over time they can degrade and so your stain won't work with some > antibodies. The weirdest part to me has always been that you don't have > to store the blocks this way. So I think that was your question, if the > blocks aren't stored in a freezer why store the slides? Won't the > antigens in the blocks start to degrade as well? This is a question I > would like to know the answer to as well... > > Sarah Goebel, B.A., HT (ASCP) > Histotechnician > > > XBiotech USA Inc. > > 8201 East Riverside Dr. Bldg 4 Suite 100 > > Austin, Texas 78744 > > (512)386-2907 > > > > > -------- Original Message -------- > Subject: Re: [Histonet] unstained paraffin tissue slides storage--why > cold? > From: Emily Sours > Date: Thu, November 04, 2010 7:07 am > To: histonet@lists.utsouthwestern.edu > > Can I ask what the point of storing paraffin sections in freezing cold > storage? > They are wax sections, which never see any type of cold, so I don't > understand the point of this. I do understand putting them at 4 degrees > to > prevent mold, but -80 seems excessive. > We have kept our slides at room temperature for years and years, but > these > slides do not have an albumin coat (which I can see getting moldy), just > a > chemical coating. > Fixing for paraffin and paraffin infiltration seems to keep antigens > safe > without refrigeration because it's so intense, but that's just > conjecture on > my part. > > Emily > -- > Outside of a dog, a book is man's best friend. Inside of a dog it's too > dark > to read. > --Groucho Marx > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dunatrsd <@t> sbcglobal.net Thu Nov 4 12:45:21 2010 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Thu Nov 4 12:45:28 2010 Subject: [Histonet] unstained paraffin tissue slides storage--why cold? In-Reply-To: References: <20101104082103.9e2d9aa830e8449a2412eb1e4f2f067e.6830c31a46.wbe@email04.secureserver.net> <3201CF51728F6048A24FA3AFFFEEF1D3178268356B@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: <146654.61671.qm@web83913.mail.sp1.yahoo.com> I did an experiment about 10 or 12?years ago, where I cut?the sections and stained them at various intervals: Day1, Day 15, 1 month, 3 months, 6 months, 1 year. Before staining: Set of slides were allowed to sit at room temp (RT). Set of slides were stored at 4C. Set of slides were vacuum sealed and stored at RT. Set of slides were vacuum sealed and stored at 4C. Slides stored at 4C consistently stained well, with a slight variance in staining after 1 year. Did not matter weather they were vacuum sealed or not. Slides left out at RT did not fare so well. Staining variability was noticed after 1 month. When the slides were stained after one year, signal was almost eliminated. Variability and loss of antigenicity was observed with the vacuum sealed slides as well. I did this experiment for just one antibody which was being extensively used at the time for one of our projects. It could be that other antibodies fare mach better under RT conditions, but why take a chance? We keep all of our control slides at 4C. Blocks are kept at RT. Thanks Dusko Trajkovic ________________________________ From: "Morken, Tim" To: Helen Fedor Cc: "histonet@lists.utsouthwestern.edu" Sent: Thu, November 4, 2010 9:09:16 AM Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? Helen, did you write a paper from that study? Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, November 04, 2010 8:56 AM To: sgoebel@xbiotech.com; Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? The study that we did showed that the staining on freshly cut slides from a block stored at room temperature was in fact not as good as slides that were sectioned 5 years earlier and stored at -20. Therefore the blocks should be stored in the cold as well. The tissue is degrading in the block and on the slides and the cold does slow down the process. The fixation in lots of the tissue is not optimal. Fixing for 48 hours will definitely? be better than the current fixation practices going on in most clinical labs,? if what you want is the best tissue preservation possible. But most of us do not have that option. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Thursday, November 04, 2010 11:21 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20).? I think the main point of doing this from what I understand is so that the antigens stay "viable".? I know over time they can degrade and so your stain won't work with some antibodies.? The weirdest part to me has always been that you don't have to store the blocks this way.? So I think that was your question, if the blocks aren't stored in a freezer why store the slides?? Won't the antigens in the blocks start to degrade as well?? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas? 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Thu Nov 4 12:54:53 2010 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Nov 4 12:55:06 2010 Subject: [Histonet] unstained paraffin tissue slides storage--why cold? In-Reply-To: <146654.61671.qm@web83913.mail.sp1.yahoo.com> References: <20101104082103.9e2d9aa830e8449a2412eb1e4f2f067e.6830c31a46.wbe@email04.secureserver.net> <3201CF51728F6048A24FA3AFFFEEF1D3178268356B@JHEMTEXVS3.win.ad.jhu.edu> <146654.61671.qm@web83913.mail.sp1.yahoo.com> Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D317826836F8@JHEMTEXVS3.win.ad.jhu.edu> Hello, We have not published the paper yet . It is in progress and hope to get it out of the pile soon. Helen From: dusko trajkovic [mailto:dunatrsd@sbcglobal.net] Sent: Thursday, November 04, 2010 1:45 PM To: Morken, Tim; Helen Fedor Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? I did an experiment about 10 or 12 years ago, where I cut the sections and stained them at various intervals: Day1, Day 15, 1 month, 3 months, 6 months, 1 year. Before staining: Set of slides were allowed to sit at room temp (RT). Set of slides were stored at 4C. Set of slides were vacuum sealed and stored at RT. Set of slides were vacuum sealed and stored at 4C. Slides stored at 4C consistently stained well, with a slight variance in staining after 1 year. Did not matter weather they were vacuum sealed or not. Slides left out at RT did not fare so well. Staining variability was noticed after 1 month. When the slides were stained after one year, signal was almost eliminated. Variability and loss of antigenicity was observed with the vacuum sealed slides as well. I did this experiment for just one antibody which was being extensively used at the time for one of our projects. It could be that other antibodies fare mach better under RT conditions, but why take a chance? We keep all of our control slides at 4C. Blocks are kept at RT. Thanks Dusko Trajkovic ________________________________ From: "Morken, Tim" To: Helen Fedor Cc: "histonet@lists.utsouthwestern.edu" Sent: Thu, November 4, 2010 9:09:16 AM Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? Helen, did you write a paper from that study? Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, November 04, 2010 8:56 AM To: sgoebel@xbiotech.com; Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? The study that we did showed that the staining on freshly cut slides from a block stored at room temperature was in fact not as good as slides that were sectioned 5 years earlier and stored at -20. Therefore the blocks should be stored in the cold as well. The tissue is degrading in the block and on the slides and the cold does slow down the process. The fixation in lots of the tissue is not optimal. Fixing for 48 hours will definitely be better than the current fixation practices going on in most clinical labs, if what you want is the best tissue preservation possible. But most of us do not have that option. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Thursday, November 04, 2010 11:21 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay "viable". I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours > Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AHutton <@t> dh.org Thu Nov 4 13:04:44 2010 From: AHutton <@t> dh.org (Hutton, Allison) Date: Thu Nov 4 13:06:45 2010 Subject: [Histonet] (no subject) Message-ID: <38A56C4F4630D348A50B3720409270870E0FE24A@dhmail.dhorg.org> I know that I am opening the er/pr/her2 can of worms again but has anyone gone as far as to educate the breast surgeons of the fixation requirements or impose any type of cutoff for when they can submit their tissues. We do not work weekends and we do have an alternate processor with a "weekend breast cycle", but we are still running into the issue of inadequately fixed breast tissue as the pathologists seem to be rushing the tissue through so we don't run over our "allotted 48 hours". Also, we do not perform er/pr/her2 in house so we are unable to validate a longer fixation time. Any advice would be greatly appreciated. Thanks Allison From anonwums1 <@t> gmail.com Thu Nov 4 13:08:03 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Nov 4 13:08:07 2010 Subject: [Histonet] Paraffin liver sections Message-ID: Dear all, One of my colleagues has consulted me about some paraffin embedded mouse livers he's been trying to cut. They were fixed in neutral buffered formalin for 3 days and then processed and embedded into paraffin. He was trying to cut 5 uM sections on our microtome, except as soon as the blade hit the liver, the liver would start to roll up and collapse into a small sliver, and you'd just get sections of paraffin with a hole where the liver should be. We tried changing cutting angle and soaking the blocks in ice water with no success. This is the first time we've tried livers, but we routinely cut decalcified bones on that microtome and have never seen that. Any ideas on what is going on? Thanks, Adam From mucram11 <@t> comcast.net Thu Nov 4 13:23:02 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Thu Nov 4 13:23:04 2010 Subject: [Histonet] Paraffin liver sections In-Reply-To: <1948971071.289097.1288894807777.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <299420067.289231.1288894982666.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> What is your processing schedule for the livers?? It is hard to know without that information. Pam Marcum UAMS ----- Original Message ----- From: "Adam ." To: histonet@lists.utsouthwestern.edu Sent: Thursday, November 4, 2010 1:08:03 PM Subject: [Histonet] Paraffin liver sections Dear all, One of my colleagues has consulted me about some paraffin embedded mouse livers he's been trying to cut. They were fixed in neutral buffered formalin for 3 days and then processed and embedded into paraffin. He was trying to cut 5 uM sections on our microtome, except as soon as the blade hit the liver, the liver would start to roll up and collapse into a small sliver, and you'd just get sections of paraffin with a hole where the liver should be. We tried changing cutting angle and soaking the blocks in ice water with no success. This is the first time we've tried livers, but we routinely cut decalcified bones on that microtome and have never seen that. Any ideas on what is going on? Thanks, Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Adam ." To: histonet@lists.utsouthwestern.edu Sent: Thursday, November 4, 2010 1:08:03 PM Subject: [Histonet] Paraffin liver sections Dear all, One of my colleagues has consulted me about some paraffin embedded mouse livers he's been trying to cut. They were fixed in neutral buffered formalin for 3 days and then processed and embedded into paraffin. He was trying to cut 5 uM sections on our microtome, except as soon as the blade hit the liver, the liver would start to roll up and collapse into a small sliver, and you'd just get sections of paraffin with a hole where the liver should be. We tried changing cutting angle and soaking the blocks in ice water with no success. This is the first time we've tried livers, but we routinely cut decalcified bones on that microtome and have never seen that. Any ideas on what is going on? Thanks, Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From afimbres <@t> uci.edu Thu Nov 4 14:15:42 2010 From: afimbres <@t> uci.edu (Fimbres, Amber) Date: Thu Nov 4 14:15:48 2010 Subject: [Histonet] Qualifications to run a Thin Prep 2000? Message-ID: Jay, I hope you are referring to the Thin Prep 2000 as being so user-friendly that anyone can do it and not belittling laboratory assistants by your reference to monkeys and bananas. Everyone that works in the laboratory is an important part of the end result--patient care. Thank you, Amber M. Fimbres, MHA, CT(ASCP)HT ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. From JWeems <@t> sjha.org Thu Nov 4 14:19:13 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Nov 4 14:19:20 2010 Subject: [Histonet] Qualifications to run a Thin Prep 2000? In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F6EE@CHEXCMS10.one.ads.che.org> Of course that's what he meant. It is wonderful - even I can run it!! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fimbres, Amber Sent: Thursday, November 04, 2010 15:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Qualifications to run a Thin Prep 2000? Jay, I hope you are referring to the Thin Prep 2000 as being so user-friendly that anyone can do it and not belittling laboratory assistants by your reference to monkeys and bananas. Everyone that works in the laboratory is an important part of the end result--patient care. Thank you, Amber M. Fimbres, MHA, CT(ASCP)HT ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From lelmgren <@t> sunriselab.com Thu Nov 4 14:23:35 2010 From: lelmgren <@t> sunriselab.com (Laurie Elmgren) Date: Thu Nov 4 14:23:58 2010 Subject: [Histonet] alcoholic formalin Message-ID: <4A672C6AE0402D4A89ECE29E8A4B47E3029E99CA@MailPDC.sunriselab.com> I have a client that uses alcoholic formalin as a primary fixative for small endometrial and cervical biopsies. What can I do to keep the cells from looking like raisins? We presently run these biopsies on a conventional processor with a short biopsy schedule. Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515x1108 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." From jaylundgren <@t> gmail.com Thu Nov 4 14:27:16 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Nov 4 14:27:23 2010 Subject: [Histonet] Qualifications to run a Thin Prep 2000? In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F6EE@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F6EE@CHEXCMS10.one.ads.che.org> Message-ID: Amber, I'm sorry if I offended anyone, I was merely referring to this instrument's wonderful ease of operation. Would you happen to know the answer to my question, as I see you are a cytotech? Jay A. Lundgren M.S., HTL (ASCP) From afimbres <@t> uci.edu Thu Nov 4 14:28:57 2010 From: afimbres <@t> uci.edu (Fimbres, Amber) Date: Thu Nov 4 14:29:02 2010 Subject: [Histonet] Qualifications to run a Thin Prep 2000? In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F6EE@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F6EE@CHEXCMS10.one.ads.che.org> Message-ID: Joyce, I was hoping that's what he meant too and wanted to clarify so it wouldn't be taken out of context. =) Amber -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Thursday, November 04, 2010 12:19 PM To: Fimbres, Amber; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Qualifications to run a Thin Prep 2000? Of course that's what he meant. It is wonderful - even I can run it!! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fimbres, Amber Sent: Thursday, November 04, 2010 15:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Qualifications to run a Thin Prep 2000? Jay, I hope you are referring to the Thin Prep 2000 as being so user-friendly that anyone can do it and not belittling laboratory assistants by your reference to monkeys and bananas. Everyone that works in the laboratory is an important part of the end result--patient care. Thank you, Amber M. Fimbres, MHA, CT(ASCP)HT ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. From Ronald.Houston <@t> nationwidechildrens.org Thu Nov 4 14:33:55 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Nov 4 14:34:03 2010 Subject: [Histonet] RE: alcoholic formalin In-Reply-To: <4A672C6AE0402D4A89ECE29E8A4B47E3029E99CA@MailPDC.sunriselab.com> References: <4A672C6AE0402D4A89ECE29E8A4B47E3029E99CA@MailPDC.sunriselab.com> Message-ID: Easy solution is to provide your client with 10% NBF Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Elmgren Sent: Thursday, November 04, 2010 3:24 PM To: Histonet Subject: [Histonet] alcoholic formalin I have a client that uses alcoholic formalin as a primary fixative for small endometrial and cervical biopsies. What can I do to keep the cells from looking like raisins? We presently run these biopsies on a conventional processor with a short biopsy schedule. Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515x1108 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From jaylundgren <@t> gmail.com Thu Nov 4 14:53:54 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Nov 4 14:53:58 2010 Subject: [Histonet] Paraffin liver sections In-Reply-To: <299420067.289231.1288894982666.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <1948971071.289097.1288894807777.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> <299420067.289231.1288894982666.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: Sounds to me like they are overprocessed (dried out). Try soaking them on ice for 30 minutes or so before cutting. Some people use a drop of fabric softener on their ice tray to soften the tissue, or there are commercial products like soft block from Polyscience. Jay A. Lundgren M.S., HTL (ASCP) From JWeems <@t> sjha.org Thu Nov 4 15:05:37 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Nov 4 15:05:43 2010 Subject: [Histonet] RE: alcoholic formalin In-Reply-To: <4A672C6AE0402D4A89ECE29E8A4B47E3029E99CA@MailPDC.sunriselab.com> References: <4A672C6AE0402D4A89ECE29E8A4B47E3029E99CA@MailPDC.sunriselab.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F71D@CHEXCMS10.one.ads.che.org> Can you get the client to use 10% NBF? That would be the first thing I'd do. If not, I'd soak blocks. One thing you can do is throw it in your water bath for a few seconds - then put on ice. The warm water seems to work really well especially on bloody tissue. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Elmgren Sent: Thursday, November 04, 2010 15:24 To: Histonet Subject: [Histonet] alcoholic formalin I have a client that uses alcoholic formalin as a primary fixative for small endometrial and cervical biopsies. What can I do to keep the cells from looking like raisins? We presently run these biopsies on a conventional processor with a short biopsy schedule. Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515x1108 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From afimbres <@t> uci.edu Thu Nov 4 15:10:40 2010 From: afimbres <@t> uci.edu (Fimbres, Amber) Date: Thu Nov 4 15:10:44 2010 Subject: [Histonet] Qualifications to run a Thin Prep 2000? In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F6EE@CHEXCMS10.one.ads.che.org> Message-ID: Jay, Our cytology laboratory is a recent convert to the Thin Prep system so I may not be able to answer your question in all of its entirety. However, our Cytyc/Hologic representative trained everyone (cytotechs and lab assistants) on how to operate the Thin Prep instruments. Cytyc had a competency form which listed who was trained and when and was then signed by the Cytyc rep. This paper is on file in our department. As to if a Thin Prep trained cytotech at your place of employment can perform the same training in place of a Cytyc representative, I'm unsure. Our Cytyc rep comes to our department almost monthly, so perhaps a friendly call to your rep would be in order? Good luck, Amber ________________________________ From: Jay Lundgren [mailto:jaylundgren@gmail.com] Sent: Thursday, November 04, 2010 12:27 PM To: Weems, Joyce Cc: Fimbres, Amber; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Qualifications to run a Thin Prep 2000? Amber, I'm sorry if I offended anyone, I was merely referring to this instrument's wonderful ease of operation. Would you happen to know the answer to my question, as I see you are a cytotech? Jay A. Lundgren M.S., HTL (ASCP) ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. From jaylundgren <@t> gmail.com Thu Nov 4 15:32:44 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Nov 4 15:32:49 2010 Subject: [Histonet] Qualifications to run a Thin Prep 2000? In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F6EE@CHEXCMS10.one.ads.che.org> Message-ID: I have sent an email to cytyc, and am awaiting a response, but I was hoping someone on Histonet knew the answer to my query. Thanks, Jay ------------------------------ > This message contains confidential information and is intended only for the > individual named. If you are not the named addressee you should not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and delete > this e-mail from your system. E-mail transmission cannot be guaranteed to be > secure or error-free as information could be intercepted, corrupted, lost, > destroyed, arrive late or incomplete, or contain viruses. The sender > therefore does not accept liability for any errors or omissions in the > contents of this message, which arise as a result of e-mail transmission. > From afimbres <@t> uci.edu Thu Nov 4 16:21:55 2010 From: afimbres <@t> uci.edu (Fimbres, Amber) Date: Thu Nov 4 16:22:02 2010 Subject: [Histonet] Qualifications to run a Thin Prep 2000? In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F6EE@CHEXCMS10.one.ads.che.org> Message-ID: I also wanted to add Jay that our lab assistants are not certified as either HT or HTL. The entry level education requirement of a lab assistant is a high school diploma (or equivalent). Of course what you want in a lab assistant is totally different (such as previous experience, knowledge of medical terminology, etc.). Thanks, Amber ________________________________ From: Jay Lundgren [mailto:jaylundgren@gmail.com] Sent: Thursday, November 04, 2010 1:33 PM To: Fimbres, Amber Cc: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Qualifications to run a Thin Prep 2000? I have sent an email to cytyc, and am awaiting a response, but I was hoping someone on Histonet knew the answer to my query. Thanks, Jay ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. From laurie.colbert <@t> huntingtonhospital.com Thu Nov 4 16:56:30 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Nov 4 16:56:34 2010 Subject: [Histonet] Coverslipping Hood Message-ID: <57BE698966D5C54EAE8612E8941D768309E91291@EXCHANGE3.huntingtonhospital.com> Does anyone know of a coverslipping hood that has a downdraft exhaust? Any recommendations on coverslipping hoods??? Laurie Colbert From JMacDonald <@t> mtsac.edu Thu Nov 4 17:15:18 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Nov 4 17:15:23 2010 Subject: [Histonet] RE: alcoholic formalin In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F71D@CHEXCMS10.one.ads.che.org> Message-ID: If they are insistent on using the alcohol try shortening or deleting some of the alcohol times on the processor. "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/04/2010 01:08 PM To Laurie Elmgren , Histonet cc Subject [Histonet] RE: alcoholic formalin Can you get the client to use 10% NBF? That would be the first thing I'd do. If not, I'd soak blocks. One thing you can do is throw it in your water bath for a few seconds - then put on ice. The warm water seems to work really well especially on bloody tissue. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Elmgren Sent: Thursday, November 04, 2010 15:24 To: Histonet Subject: [Histonet] alcoholic formalin I have a client that uses alcoholic formalin as a primary fixative for small endometrial and cervical biopsies. What can I do to keep the cells from looking like raisins? We presently run these biopsies on a conventional processor with a short biopsy schedule. Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515x1108 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ernestinemiddleton <@t> yahoo.ca Thu Nov 4 19:49:55 2010 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Thu Nov 4 19:49:58 2010 Subject: [Histonet] Automatic special stainer for Histology Message-ID: <640132.97407.qm@web51504.mail.re2.yahoo.com> To the Histology community; Need information on automatic special stainer that are being used in Histology community laboratory.? Price per slide and reliability of the instrument as well as the stains. Thank you. Ermestine Middleton, Manager Montefiore Medical Center Histology Laboratory; Dept. Pathology Bronx, New York 718-920-4157 emiddlet@montefiore.org From anonwums1 <@t> gmail.com Thu Nov 4 22:34:21 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Nov 4 22:34:28 2010 Subject: [Histonet] Paraffin liver sections In-Reply-To: References: <1948971071.289097.1288894807777.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> <299420067.289231.1288894982666.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: Thanks to all the prompt responses. He fixed the entire liver in excess formalin (I think around 15 mL) with rocking for 3 days. I'm not sure about the processing schedule; I'll have to contact the histology core to ask. I forwarded all of the advice along, and he tried soaking the blocks in ice water for several minutes. It fixed most of the problem, at least for now. You people are far too helpful. Thanks, Adam On Thu, Nov 4, 2010 at 2:53 PM, Jay Lundgren wrote: > Sounds to me like they are overprocessed (dried out). Try soaking them on > ice for 30 minutes or so before cutting. Some people use a drop of fabric > softener on their ice tray to soften the tissue, or there are commercial > products like soft block from Polyscience. > > Jay A. Lundgren M.S., > HTL (ASCP) > > > > > > > > > > From bakevictoria <@t> gmail.com Fri Nov 5 06:37:37 2010 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Nov 5 06:37:41 2010 Subject: [Histonet] Paraffin liver sections In-Reply-To: References: Message-ID: Hi - Whole mouse livers do not need to be fixed for 72 hours given the process you described. The protocol I followed for fixation of whole mouse organs was within 24 hours, also when processing I started at 70% etoh, I also included a 50/50 ratio of abs/xylene mix for my first clearing as it was not as harsh on smaller tissue samples. The times and heat on a processor will turn this tissue very brittle if not handled correctly. If you can get a better description of his processing this would be a help. When cutting trimming and soaking on ice will help, but if you put the block into warm water for about 30 to 45 seconds and then put it onto an ice slurry will also rehydrate the block enough to get a decent section as well. I hope this helps. Vikki On Thu, Nov 4, 2010 at 2:08 PM, Adam . wrote: > Dear all, > > One of my colleagues has consulted me about some paraffin embedded mouse > livers he's been trying to cut. They were fixed in neutral buffered > formalin > for 3 days and then processed and embedded into paraffin. He was trying to > cut 5 uM sections on our microtome, except as soon as the blade hit the > liver, the liver would start to roll up and collapse into a small sliver, > and you'd just get sections of paraffin with a hole where the liver should > be. We tried changing cutting angle and soaking the blocks in ice water > with > no success. This is the first time we've tried livers, but we routinely cut > decalcified bones on that microtome and have never seen that. Any ideas on > what is going on? > > Thanks, > Adam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bakevictoria <@t> gmail.com Fri Nov 5 06:52:57 2010 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Nov 5 06:53:03 2010 Subject: [Histonet] Paraffin liver sections In-Reply-To: References: Message-ID: Sorry - short addenda - depending on what they were doing with this tissue may also be critical as over processing or extended fixation may affect the tissue microscopic evaluation. Vikki On Fri, Nov 5, 2010 at 7:37 AM, Victoria Baker wrote: > Hi - > > Whole mouse livers do not need to be fixed for 72 hours given the process > you described. The protocol I followed for fixation of whole mouse organs > was within 24 hours, also when processing I started at 70% etoh, I also > included a 50/50 ratio of abs/xylene mix for my first clearing as it was not > as harsh on smaller tissue samples. The times and heat on a processor will > turn this tissue very brittle if not handled correctly. If you can get a > better description of his processing this would be a help. > > When cutting trimming and soaking on ice will help, but if you put the > block into warm water for about 30 to 45 seconds and then put it onto an ice > slurry will also rehydrate the block enough to get a decent section as > well. > > I hope this helps. > > Vikki > > On Thu, Nov 4, 2010 at 2:08 PM, Adam . wrote: > >> Dear all, >> >> One of my colleagues has consulted me about some paraffin embedded mouse >> livers he's been trying to cut. They were fixed in neutral buffered >> formalin >> for 3 days and then processed and embedded into paraffin. He was trying to >> cut 5 uM sections on our microtome, except as soon as the blade hit the >> liver, the liver would start to roll up and collapse into a small sliver, >> and you'd just get sections of paraffin with a hole where the liver should >> be. We tried changing cutting angle and soaking the blocks in ice water >> with >> no success. This is the first time we've tried livers, but we routinely >> cut >> decalcified bones on that microtome and have never seen that. Any ideas on >> what is going on? >> >> Thanks, >> Adam >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From sfeher <@t> CMC-NH.ORG Fri Nov 5 10:45:34 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Nov 5 10:45:39 2010 Subject: [Histonet] Qualifications to run a Thin Prep 2000? In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E1640449E2F6EE@CHEXCMS10.one.ads.che.org> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC2426C89A@exchange.cmc-nh.org> The distinction between the physical actions required to process a specimen using the T-2000 need to be viewed in conjunction with the overall task involved in processing gyn and non-gyn cytology. While there are no specific qualifications dictated by CAP for this task, we include it as just one part of the overall expected competencies expected for techs processing cytology specimens. ASCT has a good basic instructional program that can be purchased on their website (http://www.asct.com/) that will set some training guidelines and give you a basis for establishing the competencies you want you prep techs to maintain. There is so much more involved than just placing a filter and pouring a vial when it comes to non-gyn cytology. We have established a position that we call Pathology Technician for our techs that process cytology, help with autopsy, and in assist our histotechs. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fimbres, Amber Sent: Thursday, November 04, 2010 5:22 PM To: Jay Lundgren Cc: histonet@lists.utsouthwestern.edu; Weems, Joyce Subject: RE: [Histonet] Qualifications to run a Thin Prep 2000? I also wanted to add Jay that our lab assistants are not certified as either HT or HTL. The entry level education requirement of a lab assistant is a high school diploma (or equivalent). Of course what you want in a lab assistant is totally different (such as previous experience, knowledge of medical terminology, etc.). Thanks, Amber ________________________________ From: Jay Lundgren [mailto:jaylundgren@gmail.com] Sent: Thursday, November 04, 2010 1:33 PM To: Fimbres, Amber Cc: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Qualifications to run a Thin Prep 2000? I have sent an email to cytyc, and am awaiting a response, but I was hoping someone on Histonet knew the answer to my query. Thanks, Jay ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Fri Nov 5 10:49:01 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Nov 5 10:49:06 2010 Subject: [Histonet] dishwasher Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43EA91A04874@HPEMX3.HealthPartners.int> We recently remodeled our histology lab (YAY) and have obtained a dishwasher for our use. Would those of you who presently are using a dishwasher share what detergent you use and where you order it from? We plan to do our staining dishes, both H&E and coplin jars from hand special stains. Any helpful hints would be appreciated and thanks ahead of time!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From sfeher <@t> CMC-NH.ORG Fri Nov 5 10:52:37 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Nov 5 10:52:41 2010 Subject: [Histonet] Automatic special stainer for Histology In-Reply-To: <640132.97407.qm@web51504.mail.re2.yahoo.com> References: <640132.97407.qm@web51504.mail.re2.yahoo.com> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC2426C89C@exchange.cmc-nh.org> We are using the Dako Artisan Link and just love it. We were doing specials manually and found that the cost per slide differential, when taking employee pay and benefits into account, really wasn't that much higher. We get consistent quality plus it's a put it on and walk away system so we have basically freed up a couple of techs to do other things. We have begun using the Artisan for special stains on ThinPreps for our BAL's and some of our FNA's as well. So far the results have been outstanding. The Artisan Link also has an interface that can link the instrument with our LIS so that pathologist orders will go directly to the Artisan. The orders are brought up using barcode scanning so we will reduce the chance of keystroke errors and it will be even faster. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Thursday, November 04, 2010 8:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic special stainer for Histology To the Histology community; Need information on automatic special stainer that are being used in Histology community laboratory.? Price per slide and reliability of the instrument as well as the stains. Thank you. Ermestine Middleton, Manager Montefiore Medical Center Histology Laboratory; Dept. Pathology Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark <@t> pcnm.com Fri Nov 5 11:12:29 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Fri Nov 5 11:12:37 2010 Subject: [Histonet] Job in New Mexico Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C013C65BB@mail.pcnm.com> Pathology Consultants of New Mexico has an opportunity for a Histology Supervisor I Las Cruces, New Mexico. Duties include grossing; frozen section processing; tissue processing, embedding, microtomy, and staining; immunohistochemistry; special handling of renal, skin and lymphoma biopsies. Prioritize, direct and maintain work flow in laboratory, ensuring compliance with quality assurance and safety standards. Monitor and ensure compliance with established histology procedures and CAP inspection standards. Maintain all inspection materials, technical policies and laboratory procedure documentation. Perform equipment maintenance as needed. Maintain supply inventory. Assist with budget development and compliance, and perform cost analyses of new technologies. Requirements: Associate of Science degree in medical technology, biology, or related life science. Also requires four years of experience as a histology technician (including at least one year in a histology supervisory role), and ASCP HT certification or eligibility for ASCP HT certification in New Mexico. Please send cover letter and resume to lbraggs@pcnm.com . Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM From sgoebel <@t> xbiotech.com Fri Nov 5 11:27:13 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Nov 5 11:27:18 2010 Subject: [Histonet] Salary range Message-ID: <20101105092713.9e2d9aa830e8449a2412eb1e4f2f067e.41fa1b21bb.wbe@email04.secureserver.net> Hello all, Does anyone know what the salary range for a histology supervisor is in the Austin Texas area? Thanks Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 From jaylundgren <@t> gmail.com Fri Nov 5 12:08:03 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Nov 5 12:08:09 2010 Subject: [Histonet] Salary range In-Reply-To: <20101105092713.9e2d9aa830e8449a2412eb1e4f2f067e.41fa1b21bb.wbe@email04.secureserver.net> References: <20101105092713.9e2d9aa830e8449a2412eb1e4f2f067e.41fa1b21bb.wbe@email04.secureserver.net> Message-ID: Salaries in Texas are pretty low, which is one of the reasons I travel :) . I would guess $60-80 K / year, with Seton on the low end, and CPL on the top end. Depending on experience, of course. Who's looking for a supervisor, CPL? Jay A. Lundgren M.S., HTL (ASCP) From jaylundgren <@t> gmail.com Fri Nov 5 12:18:22 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Nov 5 12:18:28 2010 Subject: [Histonet] dishwasher In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43EA91A04874@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43EA91A04874@HPEMX3.HealthPartners.int> Message-ID: I have been places where they use regular grocery store dishwasher detergent to clean their lab glassware, and it works OK. IMHO, however, you can't beat Alconox. They make a formula for machine washing as well as one for manual washing. They are specifically designed to rinse cleanly, without leaving residue. However, for silver stains, I would still recommend another cleaning step, such as acid cleaning followed by three rinses of diH20. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) From kblack <@t> digestivehlth.com Fri Nov 5 15:08:40 2010 From: kblack <@t> digestivehlth.com (Konni Black) Date: Fri Nov 5 15:08:49 2010 Subject: [Histonet] need temporary histotech in Troy, MI Message-ID: A friend of mine asked me to post a request for a temporary histotech, available in the Troy, Michigan area, to replace her for 6 weeks while she is recuperating from surgery. Please contact me kblack@digestivehlth.com if you have an interest. Thank you, Konni Black From jdoerner <@t> gladstone.ucsf.edu Fri Nov 5 18:46:08 2010 From: jdoerner <@t> gladstone.ucsf.edu (Janine Doerner) Date: Fri Nov 5 18:46:44 2010 Subject: [Histonet] Gladstone Research Technologist III position Message-ID: <00aa01cb7d43$9e766070$db632150$@ucsf.edu> Research Technologist III Company Information: The J. David Gladstone Institutes is an independent, nonprofit organization, affiliated with UCSF, contributing to the health and well-being of all people through medical research, education, and outreach in the areas of heart disease, AIDS, and Alzheimer's disease. Gladstone is composed of three separate institutes and approximately 350 employees. Our employees receive exceptional benefits, including 3 weeks of paid vacation, medical/dental/vision coverage, tuition reimbursement, and excellent retirement programs. We are located in an award-winning building adjacent to UCSF's Mission Bay Campus. Gladstone has consistently ranked as one of the top places to work in academia in the United States. Duties and Responsibilities: The person in this position will be reporting to the Histology Core Manager. . Must be able to work within a core laboratory that provides Institutes-wide research services, to develop and implement specific plans and laboratory procedures to perform various analyses supporting the investigation of complex scientific problems. . Responsible for half the core request workload as well as development of new techniques as required by current scientific advances or requested by Gladstone researchers. . Jointly responsible for the maintenance and upkeep of core equipment. Responsible for maintenance, training and support of microscopy systems currently in the core and also in individual researchers labs around the institute Required qualifications: The candidate's expertise should include research (animal) histology techniques, animal dissection and necropsy, light microscopy (including epifluorescent, confocal and multi-photon techniques), photography, and digital and image analysis. Ability to interact with researchers on a scientific and function level. Immunohistochemistry experience is a must and in-situ hybridization experience is a plus. Experience in handling neurological samples is a strong plus. Requires a minimum of BA/BS plus 5 years relevant experience. Please send resumes to hr@gladstone.ucsf.edu and reference job #C10-08UT. Gladstone is committed to a policy that provides equal employment opportunities to all employees and applicants for employment without regard to race, color, sex, religion, national origin, ancestry, age, marital status, medical condition, physical or mental disability, veteran status, sexual orientation, or any other non-job related characteristic, and to make all employment decisions so as to further this principle of equal employment opportunity. Janine Doerner Senior Human Resources Recruiter The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 Telephone: (415) 734-2035 Fax: (415) 355-0910 E-mail: jdoerner@gladstone.ucsf.edu From zodiac29 <@t> comcast.net Sat Nov 6 07:15:52 2010 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Sat Nov 6 07:15:55 2010 Subject: [Histonet] section shrinkage during IHC Message-ID: <721690961.859758.1289045752874.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> From zodiac29 <@t> comcast.net Sat Nov 6 07:27:47 2010 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Sat Nov 6 07:27:49 2010 Subject: [Histonet] shrinkage during IHC Message-ID: <404871469.860096.1289046467522.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> To All, We are a lab that sends our specimens out for IHC and have just switched to another reference laboratory for these services. Our pathologist is saying that the tissue looks shrunk on the IHC slides, yet the slides that I process (H&E, and special stains)are fine. Does anyone know what is causing this? The reference lab said it could be the type of slides that I use to mount the sections we send to them. My knowledge in IHC is limited. Also, if this helps, they are FFPE tissue. Thanks for your help Jenny From jacquitta <@t> earthlink.net Sat Nov 6 17:59:48 2010 From: jacquitta <@t> earthlink.net (Jacquitta L Taylor) Date: Sat Nov 6 18:00:09 2010 Subject: [Histonet] 1 day old mice heads Message-ID: <4CD5DDE4.1070706@earthlink.net> I have been ask to decal ( 14% EDTA ) and process 1 day old mice heads. My investigator is looking for a phenotype in the calveria . After processing, the heads will be embedded on end then sectioned. I have never processed whole heads . I would love to hear from any body that has experienced this type of project. I'm thankful for Histonet this thanksgiving. Jackie Taylor Manager Histology Dept UMKC Dental School Kansas City Mo taylorjackie@umkc.edu From pruegg <@t> ihctech.net Sun Nov 7 09:29:38 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Nov 7 09:30:15 2010 Subject: [Histonet] FW: SPAM-LOW: The Fifth Annual International Retreat on Applied Immunohistochemistry and Molecular Pathology Message-ID: A Reminder! Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: Dr. Hadi Yaziji [mailto:info@pathlearning.com] Sent: Monday, October 04, 2010 12:55 PM To: Patsy Ruegg Subject: SPAM-LOW: The Fifth Annual International Retreat on Applied Immunohistochemistry and Molecular Pathology Dear colleague, It is my honor and pleasure to announce to you the Fifth Annual International Retreat on Applied Immunohistochemistry and Molecular Pathology (AIMP). The reason we have been offering this course for five consecutive years (and hopefully many more years to come) is because we maintained focus on the issues that matter the most to the practicing pathologist. In the way of example , the fifth course will continue to focus on the following aspects of IHC and molecular applications in our practice: 1. Among the >2000 new antibodies, can you offer a short list of new antibodies that I can benefit from their utility in surgical pathology? 2. What type of IHC platform should we bring to our pathology lab? 3. How can we effectively apply QA measures into our IHC lab and molecular lab? 4. What kind of impact do the ASCO/CAP guidelines have on our gross room workflow and documentation? 5. What do I need to do to ensure our lab is compliant with these guidelines? 6. What kind of FISH assays could our lab benefit from bringing in-house? 7. What are the most common molecular assays in oncologic pathology that I should be familiar with? 8. How do I build a molecular lab in my hospital? 9. Can you show me real - life examples of the staining pattern of antibodies I use in my practice? We constructed the educational program to address the above practical questions, as you can note from perusing the Schedule page. We assembled the largest possible group of 13 of the most experienced colleagues in the field, including Stanley Hamilton, Mahul Amin, Elizabeth Hammond, Bill Travis, David Hicks, Clive Taylor, Todd Barry, Serhan Alkan, James Burchette, Richard Cartun, Stephen Hewitt, David Young, Steve Potts, Richard Eisen, Bryan Hewlett, and myself. In addition to the regular didactics, the course offers the following program components: . Video presentation of instructive cases with ample illustrations of H&E slides and immunostains. . An open session where the participants can discuss any issue of interest at length with the audience. . A full-day workshop on technical IHC is a must-attend event for technologists and pathologists who wish to dig deep into the technical aspects of this amazing field. . "Keynote" talk by Dr. Stanley Hamilton on molecular testing of GI tumors. . "Modern pathology" talk, by Drs. David Young and Steve Potts on 3D aspects of digital slide imaging . "Special topic" talk by Dr. David Hicks on the importance of HER2 testing in gastric cancer . Antibody of the year: Ki67. We added this talk last year, which is now part of the annual curriculum to cover the most important antibodies in clinical IHC. . Anonymous pre test and post test: to assist the participants measure their learning experience from before/after. Top socring participaints will receive awards. On average, 40% of attendees are repeat attendees. Our colleagues who attended the first four courses truly enjoyed this event. In fact, many of them described it as "the most practical CME pathology course I've ever attended". We hope you will enjoy the upcoming course. It is very exciting and we look forward to seeing you there. Please visit http://pathlearning.com/The_Fifth_Annual_International_Retreat_on_Applied_Im munohistochemistry_and_Molecular_Morphology/Index.html for detailed information of the program and how to register. This year, the ballroom can only accommodate a maximum of 200 participants in classroom seating. We recommend that you register fairly soon to guarantee a spot. Best regards, Hadi Yaziji, MD Course Director Medical Director, Vitro Molecular Laboratories From pruegg <@t> ihctech.net Sun Nov 7 09:39:53 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Nov 7 09:40:33 2010 Subject: [Histonet] unstained paraffin tissue slides storage--why cold? In-Reply-To: References: <20101104082103.9e2d9aa830e8449a2412eb1e4f2f067e.6830c31a46.wbe@email04.secureserver.net><3201CF51728F6048A24FA3AFFFEEF1D3178268356B@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: I know this is anecdotal but I store precious control blocks and cut section (I do not melt the sections before storing and only melt just before staining) at 4dc, I do not have freezer room so I do this. I have definitely noticed when my techs forget and melt the paraffin in the sections and then store them that they lose some antigenicity. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, November 04, 2010 10:09 AM To: 'Helen Fedor' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? Helen, did you write a paper from that study? Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, November 04, 2010 8:56 AM To: sgoebel@xbiotech.com; Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? The study that we did showed that the staining on freshly cut slides from a block stored at room temperature was in fact not as good as slides that were sectioned 5 years earlier and stored at -20. Therefore the blocks should be stored in the cold as well. The tissue is degrading in the block and on the slides and the cold does slow down the process. The fixation in lots of the tissue is not optimal. Fixing for 48 hours will definitely be better than the current fixation practices going on in most clinical labs, if what you want is the best tissue preservation possible. But most of us do not have that option. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Thursday, November 04, 2010 11:21 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay "viable". I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mimmopozzuoli <@t> gmail.com Sun Nov 7 12:36:09 2010 From: mimmopozzuoli <@t> gmail.com (Mimmo Pozzuoli) Date: Sun Nov 7 12:36:13 2010 Subject: [Histonet] cytotechnology Message-ID: Are any histotechs out there performing FISH or automated cyto procedures in their labs? If so, are your pathologists having to screen and act as the cyto supervisors? What are the guidelines on this? Can a histotech perform cytoprep functions, or is the slide screening integral to the position? From pruegg <@t> ihctech.net Sun Nov 7 14:20:58 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Nov 7 14:21:37 2010 Subject: SPAM-LOW: [Histonet] shrinkage during IHC In-Reply-To: <404871469.860096.1289046467522.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> References: <404871469.860096.1289046467522.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: <9109304A049D40F889C150360425E41B@prueggihctechlt> You should have the same morphology from the IHC slides done outside that you get from your H&E, do you provide them with slides or the Block? The only thing I can think of is if they airdry after IHC instead of going thru alcohols and xylene to coverslip (if they use AEC or ap/red they may airdry), I have seen cell shrinkage from airdrying after IHC occasionally. Something else just occurred to me, if they over heat during HIER I suppose this could happen, but I have not seen it. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net Sent: Saturday, November 06, 2010 6:28 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] shrinkage during IHC To All, We are a lab that sends our specimens out for IHC and have just switched to another reference laboratory for these services. Our pathologist is saying that the tissue looks shrunk on the IHC slides, yet the slides that I process (H&E, and special stains)are fine. Does anyone know what is causing this? The reference lab said it could be the type of slides that I use to mount the sections we send to them. My knowledge in IHC is limited. Also, if this helps, they are FFPE tissue. Thanks for your help Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Nov 7 14:55:42 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Nov 7 14:56:23 2010 Subject: SPAM-LOW: [Histonet] cytotechnology In-Reply-To: References: Message-ID: Are you talking about interpreting the test or just performing it? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mimmo Pozzuoli Sent: Sunday, November 07, 2010 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] cytotechnology Are any histotechs out there performing FISH or automated cyto procedures in their labs? If so, are your pathologists having to screen and act as the cyto supervisors? What are the guidelines on this? Can a histotech perform cytoprep functions, or is the slide screening integral to the position? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Mon Nov 8 03:43:13 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Mon Nov 8 03:43:17 2010 Subject: [Histonet] Frozen section ...HELP Message-ID: Hi all, after more than a decade of NOT cutting frozen sections, I find myself back at the ice-face. To get my hand in (not literally) I thought I would do some trial sections on stored tissue - stuff that was in formalin and now in 70% alcohol. Horror ; dismay. The tissue, once frozen, is all mushy in the middle. Is this because of teh long storage? is there anything I can do to improve the situation? much appreciated -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From lpwenk <@t> sbcglobal.net Mon Nov 8 04:33:52 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Nov 8 04:34:01 2010 Subject: [Histonet] Frozen section ...HELP In-Reply-To: References: Message-ID: <0B2A6F65620A4565904BC1C81A1B76F9@HP2010> The freezing point of water is 0 degrees C. The freezing point of 100% ethanol is -114 degrees C. The freezing point of 70% alcohol is about -48 degrees C. Since most cryostats are at -20 to -25 degrees C, your tissue isn't freezing completely. You probably have "slush" ice inside the cells. Not hard enough to support the tissue during a frozen section. Fresh tissue would be the best, as formalin fixed tissue also tends to cut awful (whole different reason). If you can't get fresh tissue from human or animal necropsy, can you get some "parts" from a raw uncooked chicken to practice on? Save some muscle, skin, liver, etc., from tonight's supper? Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "louise renton" Sent: Monday, November 08, 2010 4:43 AM To: "Histonet" Subject: [Histonet] Frozen section ...HELP > Hi all, > > after more than a decade of NOT cutting frozen sections, I find myself > back at the ice-face. > To get my hand in (not literally) I thought I would do some trial > sections on stored tissue - stuff that was in formalin and now in 70% > alcohol. > > Horror ; dismay. The tissue, once frozen, is all mushy in the middle. > Is this because of teh long storage? > > is there anything I can do to improve the situation? > > much appreciated > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Kuhnla <@t> chsli.org Mon Nov 8 07:08:25 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Mon Nov 8 07:08:36 2010 Subject: [Histonet] shrinkage during IHC In-Reply-To: <404871469.860096.1289046467522.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> References: <404871469.860096.1289046467522.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: In my experience, IHC usually plumps tissue back up during reteival. Is this noticed with every antibody or just a few? Certain tissue types? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net Sent: Saturday, November 06, 2010 8:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shrinkage during IHC To All, We are a lab that sends our specimens out for IHC and have just switched to another reference laboratory for these services. Our pathologist is saying that the tissue looks shrunk on the IHC slides, yet the slides that I process (H&E, and special stains)are fine. Does anyone know what is causing this? The reference lab said it could be the type of slides that I use to mount the sections we send to them. My knowledge in IHC is limited. Also, if this helps, they are FFPE tissue. Thanks for your help Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From PMonfils <@t> Lifespan.org Mon Nov 8 08:14:09 2010 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Nov 8 08:14:23 2010 Subject: [Histonet] Frozen section ...HELP In-Reply-To: References: Message-ID: <4EBFF65383B74D49995298C4976D1D5E075E13A2@LSRIEXCH1.lsmaster.lifespan.org> Hopefully you removed the antifreeze (alcohol) before freezing? :-) I have run into this a few times. I work in a core facility where people send me samples from all over. Recently someone sent me some samples fixed in Histofix, which is a commercially available aqueous fixative. But they neglected to tell me they add 15% ethanol to the commercial solution. I froze all the samples in OCT compound, but they could not be sectioned. I had to melt them all, soak them in several changes of buffer for several hours to remove the OCT and the methanol, then put them in fresh OCT and refreeze them. And that was only 15% alcohol, not 70%! From dchihc <@t> yahoo.com Mon Nov 8 09:26:59 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Mon Nov 8 09:27:02 2010 Subject: [Histonet] Distance Learning Message-ID: <718649.88511.qm@web43512.mail.sp1.yahoo.com> We are thinking of?using one of the online or distance learning programs to help in the training of new histotechs. Has anyone had any experience with any of these programs,?what colleges offer the program, pros, cons. Any feedback is welcome. Thanks ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From mbrooks <@t> incytepathology.com Mon Nov 8 10:36:04 2010 From: mbrooks <@t> incytepathology.com (Matt Brooks) Date: Mon Nov 8 10:36:09 2010 Subject: [Histonet] Microwave processing effect on DNA/RNA Message-ID: <706224670091FE47997AEF88EFADE7CA0194EA35@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello All, I have seen some posts on the "possible" damaging effects on DNA and RNA by microwave processing. Does anyone have a reference article that they can share with me? We are in the process of budgeting for next year and we are looking at microwave processors; but I want to verify if this claim is or is not valid. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 From vkline2344 <@t> comcast.net Mon Nov 8 12:09:21 2010 From: vkline2344 <@t> comcast.net (vkline2344) Date: Mon Nov 8 12:11:56 2010 Subject: [Histonet] Re: 8. Distance Learning (Phyllis Thaxton) Message-ID: <75415214289D406880DB0D82BD0A22F8@vicPC> Hi Everyone, I'm new to histonet and haven't participated in any discussions yet, but I noticed the question regarding distance/online programs for histotechnicians and I'm currently enrolled in an accredited Histology Technician program through Columbus State Community College. I'd be more than happy to share any feedback as a student if you're interested. Feel free to email me privately at: vkline2344@comcast.net -Victoria Kline From JABiedermann <@t> uams.edu Mon Nov 8 14:10:49 2010 From: JABiedermann <@t> uams.edu (Biedermann, JoAnn) Date: Mon Nov 8 14:09:42 2010 Subject: [Histonet] RE: Frozen Section Help In-Reply-To: References: Message-ID: <833890C7E1BE584B926A2584E6B37ADC95FA7BA0@MAIL2.ad.uams.edu> Did you cryoprotect the tissue before you cut it? JB Jo Ann Biedermann Research Assistant University of Arkansas for Medical Sciences Reynolds Institute on Aging 629 Jack Stephens Drive Room 3173??? Mail Slot 807 Little Rock, AR 72205 Phone: 501-526-5803 FAX: 501-526-5830 JABiedermann@uams.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, November 08, 2010 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 84, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. cytotechnology (Mimmo Pozzuoli) 2. RE: SPAM-LOW: [Histonet] shrinkage during IHC (Patsy Ruegg) 3. RE: SPAM-LOW: [Histonet] cytotechnology (Patsy Ruegg) 4. Frozen section ...HELP (louise renton) 5. Re: Frozen section ...HELP (Lee & Peggy Wenk) 6. RE: shrinkage during IHC (Kuhnla, Melissa) 7. RE: Frozen section ...HELP (Monfils, Paul) 8. Distance Learning (Phyllis Thaxton) 9. Microwave processing effect on DNA/RNA (Matt Brooks) ---------------------------------------------------------------------- Message: 1 Date: Sun, 7 Nov 2010 13:36:09 -0500 From: Mimmo Pozzuoli Subject: [Histonet] cytotechnology To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Are any histotechs out there performing FISH or automated cyto procedures in their labs? If so, are your pathologists having to screen and act as the cyto supervisors? What are the guidelines on this? Can a histotech perform cytoprep functions, or is the slide screening integral to the position? ------------------------------ Message: 2 Date: Sun, 7 Nov 2010 13:20:58 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] shrinkage during IHC To: , Message-ID: <9109304A049D40F889C150360425E41B@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" You should have the same morphology from the IHC slides done outside that you get from your H&E, do you provide them with slides or the Block? The only thing I can think of is if they airdry after IHC instead of going thru alcohols and xylene to coverslip (if they use AEC or ap/red they may airdry), I have seen cell shrinkage from airdrying after IHC occasionally. Something else just occurred to me, if they over heat during HIER I suppose this could happen, but I have not seen it. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net Sent: Saturday, November 06, 2010 6:28 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] shrinkage during IHC To All, We are a lab that sends our specimens out for IHC and have just switched to another reference laboratory for these services. Our pathologist is saying that the tissue looks shrunk on the IHC slides, yet the slides that I process (H&E, and special stains)are fine. Does anyone know what is causing this? The reference lab said it could be the type of slides that I use to mount the sections we send to them. My knowledge in IHC is limited. Also, if this helps, they are FFPE tissue. Thanks for your help Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sun, 7 Nov 2010 13:55:42 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] cytotechnology To: "'Mimmo Pozzuoli'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Are you talking about interpreting the test or just performing it? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mimmo Pozzuoli Sent: Sunday, November 07, 2010 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] cytotechnology Are any histotechs out there performing FISH or automated cyto procedures in their labs? If so, are your pathologists having to screen and act as the cyto supervisors? What are the guidelines on this? Can a histotech perform cytoprep functions, or is the slide screening integral to the position? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 8 Nov 2010 11:43:13 +0200 From: louise renton Subject: [Histonet] Frozen section ...HELP To: Histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi all, after more than a decade of NOT cutting frozen sections, I find myself back at the ice-face. To get my hand in (not literally) I thought I would do some trial sections on stored tissue - stuff that was in formalin and now in 70% alcohol. Horror ; dismay. The tissue, once frozen, is all mushy in the middle. Is this because of teh long storage? is there anything I can do to improve the situation? much appreciated -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 5 Date: Mon, 8 Nov 2010 05:33:52 -0500 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] Frozen section ...HELP To: "louise renton" , "Histonet" Message-ID: <0B2A6F65620A4565904BC1C81A1B76F9@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original The freezing point of water is 0 degrees C. The freezing point of 100% ethanol is -114 degrees C. The freezing point of 70% alcohol is about -48 degrees C. Since most cryostats are at -20 to -25 degrees C, your tissue isn't freezing completely. You probably have "slush" ice inside the cells. Not hard enough to support the tissue during a frozen section. Fresh tissue would be the best, as formalin fixed tissue also tends to cut awful (whole different reason). If you can't get fresh tissue from human or animal necropsy, can you get some "parts" from a raw uncooked chicken to practice on? Save some muscle, skin, liver, etc., from tonight's supper? Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "louise renton" Sent: Monday, November 08, 2010 4:43 AM To: "Histonet" Subject: [Histonet] Frozen section ...HELP > Hi all, > > after more than a decade of NOT cutting frozen sections, I find myself > back at the ice-face. > To get my hand in (not literally) I thought I would do some trial > sections on stored tissue - stuff that was in formalin and now in 70% > alcohol. > > Horror ; dismay. The tissue, once frozen, is all mushy in the middle. > Is this because of teh long storage? > > is there anything I can do to improve the situation? > > much appreciated > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 8 Nov 2010 08:08:25 -0500 From: "Kuhnla, Melissa" Subject: RE: [Histonet] shrinkage during IHC To: , Message-ID: Content-Type: text/plain; charset="us-ascii" In my experience, IHC usually plumps tissue back up during reteival. Is this noticed with every antibody or just a few? Certain tissue types? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net Sent: Saturday, November 06, 2010 8:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shrinkage during IHC To All, We are a lab that sends our specimens out for IHC and have just switched to another reference laboratory for these services. Our pathologist is saying that the tissue looks shrunk on the IHC slides, yet the slides that I process (H&E, and special stains)are fine. Does anyone know what is causing this? The reference lab said it could be the type of slides that I use to mount the sections we send to them. My knowledge in IHC is limited. Also, if this helps, they are FFPE tissue. Thanks for your help Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ------------------------------ Message: 7 Date: Mon, 8 Nov 2010 09:14:09 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] Frozen section ...HELP To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E075E13A2@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" Hopefully you removed the antifreeze (alcohol) before freezing? :-) I have run into this a few times. I work in a core facility where people send me samples from all over. Recently someone sent me some samples fixed in Histofix, which is a commercially available aqueous fixative. But they neglected to tell me they add 15% ethanol to the commercial solution. I froze all the samples in OCT compound, but they could not be sectioned. I had to melt them all, soak them in several changes of buffer for several hours to remove the OCT and the methanol, then put them in fresh OCT and refreeze them. And that was only 15% alcohol, not 70%! ------------------------------ Message: 8 Date: Mon, 8 Nov 2010 07:26:59 -0800 (PST) From: Phyllis Thaxton Subject: [Histonet] Distance Learning To: Histonet@lists.utsouthwestern.edu Message-ID: <718649.88511.qm@web43512.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We are thinking of?using one of the online or distance learning programs to help in the training of new histotechs. Has anyone had any experience with any of these programs,?what colleges offer the program, pros, cons. Any feedback is welcome. Thanks ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ------------------------------ Message: 9 Date: Mon, 8 Nov 2010 08:36:04 -0800 From: "Matt Brooks" Subject: [Histonet] Microwave processing effect on DNA/RNA To: Message-ID: <706224670091FE47997AEF88EFADE7CA0194EA35@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Content-Type: text/plain; charset="us-ascii" Hello All, I have seen some posts on the "possible" damaging effects on DNA and RNA by microwave processing. Does anyone have a reference article that they can share with me? We are in the process of budgeting for next year and we are looking at microwave processors; but I want to verify if this claim is or is not valid. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 84, Issue 9 *************************************** Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From TJJ <@t> stowers.org Mon Nov 8 14:19:18 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Nov 8 14:19:27 2010 Subject: [Histonet] Re: Microwave processing effect on DNA/RNA Message-ID: Matt, You asked an interesting question and it got me to googling. Since microwave radiation is non-ionizing it should not adversely affect things like DNA and RNA. I found this summary of a group of publications on this and apparently in 1995, Kakita demonstrated that microwaves were capable of fragmenting viral DNA, and they were able to conclude it was not due to the heating effect. http://www.rfsafe.com/research/rf_hazards/dna_damage/microwave_effect.htm Most of the data I can find seems to correspond to the type of electromagnetic radiation produced by cell phones, because that's the biggie going around these days. Here's a more recent paper on cell phone microwaves and their effect on human lymphocytes. It might be useful to check out the reference list, especially the ones cited in the introduction. http://www.hese-project.org/hese-uk/en/papers/sarimov_chromatin_heatshock_ieee04.pdf The thing I don't know is how the microwaves from cell phones equate, or if they even do, with microwave oven exposure, especially since we can control the samples from being overheated. Also, this study was done on cell cultures, which while it might give interesting and useful information, it does not mimic the conditions of a whole organism. In addition, the microwave effects were reported on unfixed cells, unlike the samples you will be processing. What I would do if I were you is get a demo unit in, simulaneously process 10-20 different patient samples with the microwave processor and with your routine processor after your routine fixation, and then send them off for analysis. That should tell you all you need to know right there. If anyone has already done this, please speak up! Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From JPeters <@t> bostwicklaboratories.com Mon Nov 8 14:36:08 2010 From: JPeters <@t> bostwicklaboratories.com (Justin Peters) Date: Mon Nov 8 14:36:13 2010 Subject: [Histonet] p53 cytology controls Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F150364DC@mail1.BOSTWICK.COM> We receive requests from our pathologists for p53 IHC on urine but I am unsure as to what controls to use. We currently use a FFPE tissue control (I understand that it is not correct) but up until now this issue has not been addressed. Can anyone help with good cytology controls? Thanks. From marktarango <@t> gmail.com Mon Nov 8 14:51:06 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Nov 8 14:51:12 2010 Subject: [Histonet] p53 cytology controls In-Reply-To: <24D22DE9E488AA43BF92A4389F2DDB1F150364DC@mail1.BOSTWICK.COM> References: <24D22DE9E488AA43BF92A4389F2DDB1F150364DC@mail1.BOSTWICK.COM> Message-ID: Hi Justin, Do you run the p53 on a cell block or a cytospin or something different? Mark On Mon, Nov 8, 2010 at 12:36 PM, Justin Peters < JPeters@bostwicklaboratories.com> wrote: > We receive requests from our pathologists for p53 IHC on urine but I am > unsure as to what controls to use. We currently use a FFPE tissue > control (I understand that it is not correct) but up until now this > issue has not been addressed. Can anyone help with good cytology > controls? Thanks. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Tue Nov 9 09:04:44 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Nov 9 09:04:50 2010 Subject: [Histonet] RELIA Special Job Alert for Managers and Supervisors. 11-09-10 Message-ID: Hello Histonetters! I have several exciting opportunities for experienced Managers, and Supervisors in hospital and private lab environments in several locations nationwide. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clients offer excellent compensation, benefits and relocation assistance. Here are my Management Opportunities: Histology Supervisor ? Colorado Springs, CO Core Lab Director ? Little Rock, AR Histology Manager ? Modesto, CA QA Manager ? Orange/Rockland County, NY If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam RELIA is offering a $500 referral bonus for anyone you refer and I place and a $500 hiring bonus if I place you! There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 8 years I have dedicated my practice solely to placing histology professionals like you. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Lynn.Burton <@t> Illinois.gov Tue Nov 9 11:17:43 2010 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Tue Nov 9 11:18:09 2010 Subject: [Histonet] looking for references to stain protocols Message-ID: I am in need of references for stain protocols. The stain I am searching for in particular is the modified Gimenez, also known as Pierce-Vanderkamp for chlamydia and rickettsia. If anyone can point me in the right direction I would appreciate it. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From rjr6 <@t> psu.edu Tue Nov 9 12:44:52 2010 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Tue Nov 9 12:45:17 2010 Subject: [Histonet] RE: looking for references to stain protocols In-Reply-To: References: Message-ID: This is the reference that I have for this stain. Andersen A.A. & VanRompay D (2005). Chlamydiosis. In: A Laboratory Manual for the Isolation and Identification of Avian Pathogens, Fifth Edition. Submitted USA. Roberta Horner HT/HTL Animal Diagnostic La Penn State University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Tuesday, November 09, 2010 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] looking for references to stain protocols I am in need of references for stain protocols. The stain I am searching for in particular is the modified Gimenez, also known as Pierce-Vanderkamp for chlamydia and rickettsia. If anyone can point me in the right direction I would appreciate it. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jaclyn_Bhamornsiri <@t> vwr.com Tue Nov 9 13:00:43 2010 From: Jaclyn_Bhamornsiri <@t> vwr.com (Jaclyn_Bhamornsiri@vwr.com) Date: Tue Nov 9 13:00:50 2010 Subject: [Histonet] Jaclyn Bhamornsiri - Important Message Message-ID: I will be out of the office starting 11/09/2010 and will not return until 12/31/2014. As of Nov 9th, 2010, I am no longer with VWR. If you need assistance, pls contact VWR Customer Service at 1800-932-5000 (or vwrcustomerservice@vwr.com). For more urgent matters, pls contact Leize Lessig at 770-733-8190 (or leize_lessig@vwr.com). Thanks! Jackie B. From Lesley.Bechtold <@t> jax.org Tue Nov 9 13:14:48 2010 From: Lesley.Bechtold <@t> jax.org (Lesley Bechtold) Date: Tue Nov 9 13:14:56 2010 Subject: [Histonet] Histology Lab Survey Message-ID: <3BDA51FFD1A83B4E90829F594A5C371743316EECB8@JAXBHMAIL01.jax.org> Dear Histonetters, Scientific Services at The Jackson Laboratory is conducting benchmarking surveys in an effort to develop an understanding of the current best practices in operations, management and technical delivery at peer institutions. The goal is to gather quality data that can be used to assist us and all survey participants with operational assessment and planning. Survey participants will receive compiled results which will be de-identified before distribution. If you would like to participate, just click on the link below and answer the questions in the survey. Additionally, for those of you who do EM, there is a link to an EM Facility survey as well. It will only take a few minutes of your time. With thanks in advance! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) Histo Survey - http://www.surveymonkey.com/s/5RPSYG2 EM Survey - http://www.surveymonkey.com/s/5RGYH9W From lbustamante <@t> cvm.tamu.edu Tue Nov 9 12:34:59 2010 From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante) Date: Tue Nov 9 13:17:18 2010 Subject: [Histonet] Cutting and embedding Message-ID: <4CD93FF3020000B9000E58DC@CVM.TAMU.EDU> Could you please tell me what is the average for embedding surgical blocks in one hour? Is there an average for cutting? Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 From pruegg <@t> ihctech.net Tue Nov 9 14:29:37 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Nov 9 14:30:17 2010 Subject: [Histonet] NSH Validation WS attendees Message-ID: <78B71368E2844D3E9B0E2253896F8291@Patsyoffice> For those of you who attended the IHC ws at NSH on Validation by Tim Morken and Patsy Ruegg we have posted the rest of the information we promised you to my ftp://ihctech.net site for you to download. We provided the user name and pass word you will need in the workshop. The folder is labeled "Tim Morken" and you can download the files to your computer. Remember this site is for uploading and downloading not opening. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From cbass <@t> wfubmc.edu Tue Nov 9 15:51:02 2010 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Tue Nov 9 15:52:17 2010 Subject: [Histonet] how to fix floating sections? Message-ID: Hello Everyone, I have a bunch of rat brains that I have frozen in isopentane and stored at ?80. I'd like to collect 300 micron sections on a cryostat and collect tissue punches for RNA isolation. I would then like to take the remaining tissue and stain. There are a few ways of doing this, what would work the best is to take two 50 micron section, followed by a 300 micron, followed by two more 50, and go through the brain serially. I will then stain the 50 micron sections with nissl or thionin, so that the structures are easily identified and then use these to direct the tissue punches. The second 50 micron sections would be reserved for GFP IHC. Is this feasible? Here are some direct questions: 1. how would I store the 300 micron sections? 2. Any suggestions/protocols for staining the 50 micron sections for structure? Which stain would you use? 3. Can I post-fix the 50 micron sections? How about the 300 micron? One reason I want to use floating sections for the nissl/thionin is that I can mount these very flat, no wrinkles and I'm very comfortable working with floating sections. I don't want to risk precious tissue trying to optimize this. One methodology recommended to me is to freeze a formalin solution in a 24 well plate, put the section on top flat and let them thaw together at room temperature or 4 degrees. This gives the tissue a nice, slow fixation. Any other suggestions? How about working with the RNA? I'm thinking of using RNAlater-ice for example. Does anyone know if this screws up native fluorescence of GFP? Thanks! Caroline Bass From cfrmd1 <@t> gmail.com Tue Nov 9 16:47:21 2010 From: cfrmd1 <@t> gmail.com (Carlos Rodriguez, MD) Date: Tue Nov 9 16:47:25 2010 Subject: [Histonet] Part-time Lab opportunity in Scottsdale, AZ Message-ID: Hi all. We have a part-time job opportunity for someone interested primarily in accessioning and grossing skin specimens at our in-house dermatopathology lab (North Scottsdale Dermatology). A small amount of general lab management will also be required (ie, maintaining CLIA logs, ordering supplies etc). The hours needed will likely be Monday, Wednesday and Thursday mornings (~5 or 6am-9am). The start date will be around late December. The hours and start date are flexible, and the workload is very reasonable. Qualifications are either HT certification, or at a minimum an Associate's Degree in a health-related field with college-level credit in Chemistry, Biology, and Math, in addition to work experience in grossing and accessioning skin specimens. If interested, please respond to me by email and include a CV and/or description of your qualifications. Thanks! Carlos Rodriguez, MD From mmashore <@t> vapop.ucsd.edu Tue Nov 9 16:56:49 2010 From: mmashore <@t> vapop.ucsd.edu (Michael Mashore) Date: Tue Nov 9 17:02:09 2010 Subject: [Histonet] Paraffin Tissue Crumbles Message-ID: <652586E049884D8491A0E70448595D40@vandeberg> Hello Histonet Users, I have just started using paraffin and am having many difficulties. Most of the time my tissue crumbles when sectioning. I have no real experience in paraffin histology and have been given the task of becoming proficient by myself, so I am hoping for feedback as to why my tissue keeps crumbling. The tissue in question has been: skeletal muscle, cardiac muscle, liver, and brain (all from rat). The tissue was fixed in 10% neutral buffered formalin for 7 days at 4?C and then transferred to an automated tissue processor, with the following schedule: 2 hours 70% dehydration alcohol 2 hours 80% dehydration alcohol 2 hours 95% dehydration alcohol 2.5 hours 95% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol .5 hour Hemo-De .5 hour Hemo-De .5 hour Hemo-De 1 hour paraffin 4 hours paraffin They were infiltrated for 1 hour without vacuum then embedded. The blocks were stored in the freezer before cutting. The knife angle was 5?. Sections were 5?m thick. I would appreciate any feedback whatsoever. Thank you very much. Michael From billodonnell <@t> catholichealth.net Tue Nov 9 17:39:27 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Nov 9 17:39:42 2010 Subject: [Histonet] Histo data needed Message-ID: Those who work in similar labs please help me out: Could anyone tell me how a clinical histology lab that do 6500 cases a year is staffed. (Average aboout 70 blocks a day, 20-30% small biopsies that require 3 levels so about 120-140 "ribbons" a day) We do not do cyto processing, we have automated H&E.and coverslipping. All special stains and immuno is does manually, but volume is low, 2-4 SS a week and maybe 6 antibodies. We do gross probalbly 60-70% of cases and assist at gross for the rest. We have no aides and are staffed @ 1.5 FTE. I'm trying to justify some help, but the suits need numbers to crunch. I hope that is enough info for comparison. Thanks in advance, Bill 'the tired tech" O'Donnell Kearrney, NE From joelleweaver <@t> hotmail.com Wed Nov 10 04:47:57 2010 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Nov 10 04:48:02 2010 Subject: [Histonet] Distance Learning In-Reply-To: <718649.88511.qm@web43512.mail.sp1.yahoo.com> References: <718649.88511.qm@web43512.mail.sp1.yahoo.com> Message-ID: Hi Phyllis We offer an on line, distance learning program from Columbus State Community Colllege that is fully accredited by NAACLS. It awards a certificate in histotechnology and meets the training and eligibility requirements for the ASCP HT registry examination. The certificate program is linked to an Associates degree in the Department of Allied and Multi-competency Health for those wanting a degree option. We are currently accepting students for the next program year. Our application and admissions process and application form is available from our program web page at www.cscc.edu/histology We are looking to expand our market and meet the needs of students and laboratories outside of Ohio. We have a few clinical affiliates in the Midwest, other than Ohio, and we would like to extend this further into under-served areas, ( as far as histology schools). We are able to offer 24/7 access to materials, instruction in theory and practice, practical work assessment, and interactive delivery via Blackboard learning platform. In the future we plan to offer continuing education opportunities that are histology specific to those aready certified or who may participate in the ASCP- CMP program ,and want to meet the continuing education requirements needed for participation. If I may answer any questions about histology training, certification or the on line study of histology, please feel free to contact me at jweave01@cscc.edu or at 614-287-2217. Joelle Weaver HTL (ASCP) > Date: Mon, 8 Nov 2010 07:26:59 -0800 > From: dchihc@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Distance Learning > > We are thinking of using one of the online or distance learning programs to help > in the training of new histotechs. Has anyone had any experience with any of > these programs, what colleges offer the program, pros, cons. Any feedback is > welcome. > > Thanks > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From f.finlay <@t> formed.gla.ac.uk Wed Nov 10 08:24:32 2010 From: f.finlay <@t> formed.gla.ac.uk (Finlay Finlay) Date: Wed Nov 10 08:24:38 2010 Subject: [Histonet] Oil Red O trivia Message-ID: Hello Just wondering if anyone knows the what the 'O' in oil red O stands for. I suspect that it stands for nothing other than O similar to the 'G' in Orange G. One of our pathologists is looking for a tricky stain question to annoy trainees with. Thanks for your help Finlay From mcauliff <@t> umdnj.edu Wed Nov 10 08:39:10 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Nov 10 08:36:19 2010 Subject: [Histonet] Oil Red O trivia In-Reply-To: References: Message-ID: <4CDAAE8E.20102@umdnj.edu> So, the pathologist does not know the answer but he wants to annoy trainees? Glad I don't work for him! Geoff Finlay Finlay wrote: > Hello > > Just wondering if anyone knows the what the 'O' in oil red O stands for. > I suspect that it stands for nothing other than O similar to the 'G' in > Orange G. One of our pathologists is looking for a tricky stain question > to annoy trainees with. > > Thanks for your help > > Finlay > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Anna.Inman <@t> stmarygj.org Wed Nov 10 08:45:28 2010 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Wed Nov 10 08:45:33 2010 Subject: [Histonet] Oil Red O control In-Reply-To: <4CDAAE8E.20102@umdnj.edu> References: <4CDAAE8E.20102@umdnj.edu> Message-ID: <2925AE271EAAD440AF48FCCEB8002D091443E583@smgmail01.smgj.sclhs.net> Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From alyssa <@t> alliedsearchpartners.com Wed Nov 10 08:51:19 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Nov 10 08:51:26 2010 Subject: [Histonet] Sending Slides Out in St. Louis, MO Message-ID: Hello, A friend of mine who has a brand new laboratory in St. Louis, MO currently has their histotech prepare their slides but are looking for a laboratory to have them read at because they do not have a Dermatopathologist. Please contact me if you have lab in the area who would be interested in doing that for them. Thank you! -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From 41dmb41 <@t> gmail.com Wed Nov 10 08:54:46 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Wed Nov 10 08:55:11 2010 Subject: [Histonet] Oil Red O control In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D091443E583@smgmail01.smgj.sclhs.net> References: <4CDAAE8E.20102@umdnj.edu> <2925AE271EAAD440AF48FCCEB8002D091443E583@smgmail01.smgj.sclhs.net> Message-ID: There was a discussion on this recently, so you may want to search the archives for it. In my opinion, the easiest and best control to use is Mayo. Just smear it on a slide as you need it and there you go. Cheap and easy. Drew On Wed, Nov 10, 2010 at 09:45, Inman, Anna wrote: > Hello - > > How does everyone handle having a positive control for Oil Red O - We > very infrequently have this stain and have had difficulty keeping > control on hand. > > > > > > Thank you > > Anna > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sun <@t> pathology.ufl.edu Wed Nov 10 09:11:41 2010 From: sun <@t> pathology.ufl.edu (Sun,Yuping) Date: Wed Nov 10 09:12:28 2010 Subject: [Histonet] Oil Red O control In-Reply-To: References: <4CDAAE8E.20102@umdnj.edu> <2925AE271EAAD440AF48FCCEB8002D091443E583@smgmail01.smgj.sclhs.net> Message-ID: <530A57D46C74F34B862F95E3F4DFF3228D5D2722@HSC-CMS01.ad.ufl.edu> Hello Histonet Users, I have a question about Oil Red O staining. Could the Oil Red O solution(Poly Scientific, Cat.No. S1848) be reused? I would appreciate any feedback whatsoever. Thank you very much. Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Wednesday, November 10, 2010 9:55 AM To: Inman, Anna Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil Red O control There was a discussion on this recently, so you may want to search the archives for it. In my opinion, the easiest and best control to use is Mayo. Just smear it on a slide as you need it and there you go. Cheap and easy. Drew On Wed, Nov 10, 2010 at 09:45, Inman, Anna wrote: > Hello - > > How does everyone handle having a positive control for Oil Red O - We > very infrequently have this stain and have had difficulty keeping > control on hand. > > > > > > Thank you > > Anna > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Wed Nov 10 09:16:43 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Nov 10 09:16:45 2010 Subject: [Histonet] Paraffin Tissue Crumbles In-Reply-To: <652586E049884D8491A0E70448595D40@vandeberg> References: <652586E049884D8491A0E70448595D40@vandeberg> Message-ID: <449016B996B2D4CDC661E7FC@CDYwxp1931.ad.med.buffalo.edu> Having some experience processing and embedding rodent tissues myself (by hand), I would say that you are over-dehydrating the tissues. Try cutting back the alcohol incubation times to 30 min or even 10 min each. Regards, Merced --On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore wrote: > Hello Histonet Users, > > > > I have just started using paraffin and am having many difficulties. Most > of the time my tissue crumbles when sectioning. I have no real experience > in paraffin histology and have been given the task of becoming proficient > by myself, so I am hoping for feedback as to why my tissue keeps > crumbling. The tissue in question has been: skeletal muscle, cardiac > muscle, liver, and brain (all from rat). > > > > The tissue was fixed in 10% neutral buffered formalin for 7 days at 4?C > and then transferred to an automated tissue processor, with the following > schedule: > > > > 2 hours 70% dehydration alcohol > > 2 hours 80% dehydration alcohol > > 2 hours 95% dehydration alcohol > > 2.5 hours 95% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > .5 hour Hemo-De > > .5 hour Hemo-De > > .5 hour Hemo-De > > 1 hour paraffin > > 4 hours paraffin > > > > They were infiltrated for 1 hour without vacuum then embedded. > > The blocks were stored in the freezer before cutting. > > The knife angle was 5?. > > Sections were 5?m thick. > > > > I would appreciate any feedback whatsoever. > > > > Thank you very much. > > > > Michael > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From JWeems <@t> sjha.org Wed Nov 10 09:20:27 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Nov 10 09:20:37 2010 Subject: [Histonet] Oil Red O control In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D091443E583@smgmail01.smgj.sclhs.net> References: <4CDAAE8E.20102@umdnj.edu> <2925AE271EAAD440AF48FCCEB8002D091443E583@smgmail01.smgj.sclhs.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044A071B7A@CHEXCMS10.one.ads.che.org> Smear mayonnaise on a slide.. It works well. And of course - not the fat free kind! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Inman, Anna Sent: Wednesday, November 10, 2010 09:45 To: Geoff McAuliffe; Finlay Finlay Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O control Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From flnails <@t> texaschildrens.org Wed Nov 10 09:25:47 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Nov 10 09:26:06 2010 Subject: [Histonet] Paraffin Tissue Crumbles In-Reply-To: <449016B996B2D4CDC661E7FC@CDYwxp1931.ad.med.buffalo.edu> References: <652586E049884D8491A0E70448595D40@vandeberg> <449016B996B2D4CDC661E7FC@CDYwxp1931.ad.med.buffalo.edu> Message-ID: I agree most of your times are too long, but you can still get sections if you put water on your ice block and allow the tissue to rehydrate a bit or put a little ammonium hydroxide and water on your ice and sit your blocks to be section in this solution. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Wednesday, November 10, 2010 9:17 AM To: Michael Mashore; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Tissue Crumbles Having some experience processing and embedding rodent tissues myself (by hand), I would say that you are over-dehydrating the tissues. Try cutting back the alcohol incubation times to 30 min or even 10 min each. Regards, Merced --On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore wrote: > Hello Histonet Users, > > > > I have just started using paraffin and am having many difficulties. > Most of the time my tissue crumbles when sectioning. I have no real > experience in paraffin histology and have been given the task of > becoming proficient by myself, so I am hoping for feedback as to why > my tissue keeps crumbling. The tissue in question has been: skeletal > muscle, cardiac muscle, liver, and brain (all from rat). > > > > The tissue was fixed in 10% neutral buffered formalin for 7 days at > 4?C and then transferred to an automated tissue processor, with the > following > schedule: > > > > 2 hours 70% dehydration alcohol > > 2 hours 80% dehydration alcohol > > 2 hours 95% dehydration alcohol > > 2.5 hours 95% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > .5 hour Hemo-De > > .5 hour Hemo-De > > .5 hour Hemo-De > > 1 hour paraffin > > 4 hours paraffin > > > > They were infiltrated for 1 hour without vacuum then embedded. > > The blocks were stored in the freezer before cutting. > > The knife angle was 5?. > > Sections were 5?m thick. > > > > I would appreciate any feedback whatsoever. > > > > Thank you very much. > > > > Michael > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From STACEY.LANGENBERG <@t> UCDENVER.EDU Wed Nov 10 09:30:22 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Wed Nov 10 09:32:51 2010 Subject: [Histonet] Sending Slides Out in St. Louis, MO In-Reply-To: References: Message-ID: <1F70FCBB6D4EC549B2ADF69B9F9EAC0348F696A29E@STEAMBOAT.ucdenver.pvt> Hi Alyssa, We are not in St Louis but I work for a really great Dermpath here in Colorado who might read them for you. "People are not an interruption of our business. People are our business." Stacey Langenberg HT (ASCP) QIHC Laboratory Manager Histology/IF CU Dermatopathology Consultants 1999 N. Fitzsimons Pkwy Suite 120 Aurora, CO 80045 Lab-720-859-3559 Fax- 303-344-0789 Office- 303-577-2303 Cell-970-405-7742 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson [alyssa@alliedsearchpartners.com] Sent: Wednesday, November 10, 2010 7:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sending Slides Out in St. Louis, MO Hello, A friend of mine who has a brand new laboratory in St. Louis, MO currently has their histotech prepare their slides but are looking for a laboratory to have them read at because they do not have a Dermatopathologist. Please contact me if you have lab in the area who would be interested in doing that for them. Thank you! -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Wed Nov 10 10:00:57 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Nov 10 10:01:02 2010 Subject: [Histonet] Position - Muscle Enzyme Histochem Tech Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A81A3@wlmmsx01.nemours.org> Hello Histonetters: We presently have a position available for a HT/HTL (certified, or eligible) in our clinical muscle enzyme histochemistry lab (within the Department of Biomedical Research). We request 3 yrs experience minimum in EHC... and skills for manual IF, as well. If you meet these requirements and have interest, please contact cbarone@nemours.org. We are looking for you! From alisha <@t> ka-recruiting.com Wed Nov 10 10:55:24 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Nov 10 10:54:59 2010 Subject: [Histonet] NH Histology Job Opportunity Message-ID: <607630384.1289408124244.JavaMail.cfservice@SL4APP4> Hi Histonet Members, I am currently working on a Histology Supervisor and Histology Technologist job opportunity in Southern NH. These job opportunities are with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salray and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From alisha <@t> ka-recruiting.com Wed Nov 10 10:57:53 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Nov 10 10:57:37 2010 Subject: [Histonet] New York Dermpath Lab Manager Message-ID: <777306831.1289408273737.JavaMail.cfservice@SL4APP4> Hi Histonet Members, I am currently working on a Laboratory Manager position in NY. the ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in dermatopathology. This job opportunity is with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From alisha <@t> ka-recruiting.com Wed Nov 10 10:58:16 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Nov 10 10:58:24 2010 Subject: [Histonet] Laboratory Manager position in MI Message-ID: <1327844936.1289408296253.JavaMail.cfservice@SL4APP1> Hi Histonet Members, I am currently working on a Laboratory Manager position in MIY. the ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in dermatopathology. This job opportunity is with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From alisha <@t> ka-recruiting.com Wed Nov 10 10:59:26 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Nov 10 10:59:35 2010 Subject: [Histonet] Histology Supervisor Job in Oklahoma Message-ID: <768330664.1289408366500.JavaMail.cfservice@SL4APP1> Hi Histonet Members, I am currently working on a Histology Supervisor position in OK. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From alisha <@t> ka-recruiting.com Wed Nov 10 11:00:45 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Nov 10 11:00:34 2010 Subject: [Histonet] Histology Supervisor Position in GA Message-ID: <2144143006.1289408445189.JavaMail.cfservice@sl4app2> Hi Histonet Members, I am currently working on a Histology Supervisor position in GA. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. The candidate must also be interested/willing to live in a rural area but work in a large teaching hospital (600+ beds). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From alisha <@t> ka-recruiting.com Wed Nov 10 11:02:27 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Nov 10 11:02:22 2010 Subject: [Histonet] Histotech Job Opportunities in NH, NY, NV, TN Message-ID: <599665792.1289408546955.JavaMail.cfservice@SL4APP4> Hi Histonet Members, I am currently working on a several histotech job opportunities in various areas across the US. I have job opportunities in NH, NY, MI, OK, GA, NV, TN, and MA. My clients are willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From nancy_schmitt <@t> pa-ucl.com Wed Nov 10 11:12:38 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Nov 10 11:13:03 2010 Subject: [Histonet] Distance Learning In-Reply-To: <20101108180327.3400616F3F8@mail.pa-ucl.com> References: <20101108180327.3400616F3F8@mail.pa-ucl.com> Message-ID: <737BD0BF52F0744B96B74B61756AC06443B2ADFB4E@hestia.ad.pa-ucl.com> Message: 8 Date: Mon, 8 Nov 2010 07:26:59 -0800 (PST) From: Phyllis Thaxton Subject: [Histonet] Distance Learning To: Histonet@lists.utsouthwestern.edu Message-ID: <718649.88511.qm@web43512.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We are thinking of?using one of the online or distance learning programs to help in the training of new histotechs. Has anyone had any experience with any of these programs,?what colleges offer the program, pros, cons. Any feedback is welcome. Thanks ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ______________________________________________________- Hi Phyllis- We have had several people go through the program at IUPUI (Indiana University/Purdue University at Indianapolis), the director is Debbie Wood and she does a great job!! People are prepared to take the BOR when finished with the program. I am including her contact information. demwood@iupui.edu Good Luck Nancy Schmitt HT, MLT (ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From jsantiago <@t> bellsouth.net Wed Nov 10 11:43:58 2010 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Wed Nov 10 11:44:06 2010 Subject: [Histonet] Telomerase h-TERT Message-ID: <358498.74474.qm@web180805.mail.gq1.yahoo.com> Is anyone using the h-TERT (Telomerase) antibody with success? If so, can you share your information/protocol with me. Thanks, Jerry Santiago, BS, HTL(ASCP)QIHC Pathology Technologist Shands Jacksonville jerry.santiago@jax.ufl.edu 904-244-6149 From becky.garrison <@t> jax.ufl.edu Wed Nov 10 13:00:45 2010 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Wed Nov 10 13:00:50 2010 Subject: [Histonet] Position - Muscle Enzyme Histochem Tech In-Reply-To: <37E4BAC017F57141AF64FAA5AEB04CE8033A81A3@wlmmsx01.nemours.org> References: <37E4BAC017F57141AF64FAA5AEB04CE8033A81A3@wlmmsx01.nemours.org> Message-ID: What state? Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Wednesday, November 10, 2010 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Position - Muscle Enzyme Histochem Tech Hello Histonetters: We presently have a position available for a HT/HTL (certified, or eligible) in our clinical muscle enzyme histochemistry lab (within the Department of Biomedical Research). We request 3 yrs experience minimum in EHC... and skills for manual IF, as well. If you meet these requirements and have interest, please contact cbarone@nemours.org. We are looking for you! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicholer <@t> slonepartners.com Wed Nov 10 13:20:55 2010 From: nicholer <@t> slonepartners.com (Nichole Ramis) Date: Wed Nov 10 13:21:04 2010 Subject: [Histonet] Re: Histology Technical Supervisor (Chicago, IL) Message-ID: <038501cb810c$65a23130$30e69390$@slonepartners.com> Slone Partners seeks a Histology Technical Supervisor for a growing laboratory affiliated with a large Health Care Organization in a Western suburb of Chicago. The ideal candidate will be HTL certified with a 4 year degree and management or supervisory experience in a busy histology laboratory. Please note that this client requires a minimum of a 4 year degree to be considered for this role. Special feature of this position: This client offers competitive wages, superior benefits and opportunities for advancement. If you meet these requirements and wish to be considered for this position, submit your resume to Darcy Bloch at darcyb@slonepartners.com . If you have experience in the diagnostic laboratory industry and wish to be considered for other roles, please forward your resume to Tara Kochis at tara@slonepartners.com. All inquiries are kept confidential From john_ronan <@t> merck.com Wed Nov 10 13:36:50 2010 From: john_ronan <@t> merck.com (Ronan, John) Date: Wed Nov 10 13:37:08 2010 Subject: [Histonet] Processing rodent tissue In-Reply-To: References: Message-ID: <2D5B2393C4946A44A7E0DA7F1A2F63AF0AA3F3@usctmx1141.merck.com> I would just add that you do not need more than 2 hours total time in paraffin before embedding. Date: Wed, 10 Nov 2010 10:16:43 -0500 From: Merced M Leiker Subject: Re: [Histonet] Paraffin Tissue Crumbles To: Michael Mashore , histonet@lists.utsouthwestern.edu Message-ID: <449016B996B2D4CDC661E7FC@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=utf-8; format=flowed Having some experience processing and embedding rodent tissues myself (by hand), I would say that you are over-dehydrating the tissues. Try cutting back the alcohol incubation times to 30 min or even 10 min each. Regards, Merced --On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore wrote: > Hello Histonet Users, > > > > I have just started using paraffin and am having many difficulties. Most > of the time my tissue crumbles when sectioning. I have no real experience > in paraffin histology and have been given the task of becoming proficient > by myself, so I am hoping for feedback as to why my tissue keeps > crumbling. The tissue in question has been: skeletal muscle, cardiac > muscle, liver, and brain (all from rat). > > > > The tissue was fixed in 10% neutral buffered formalin for 7 days at 4??C > and then transferred to an automated tissue processor, with the following > schedule: > > > > 2 hours 70% dehydration alcohol > > 2 hours 80% dehydration alcohol > > 2 hours 95% dehydration alcohol > > 2.5 hours 95% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > .5 hour Hemo-De > > .5 hour Hemo-De > > .5 hour Hemo-De > > 1 hour paraffin > > 4 hours paraffin > > > > They were infiltrated for 1 hour without vacuum then embedded. > > The blocks were stored in the freezer before cutting. > > The knife angle was 5??. > > Sections were 5??m thick. > > > > I would appreciate any feedback whatsoever. John Ronan Research Associate Bioanalytics & Pathology 732 594-6378 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, November 10, 2010 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 84, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: looking for references to stain protocols (Roberta Horner) 2. Jaclyn Bhamornsiri - Important Message (Jaclyn_Bhamornsiri@vwr.com) 3. Histology Lab Survey (Lesley Bechtold) 4. Cutting and embedding (Lin Bustamante) 5. NSH Validation WS attendees (Patsy Ruegg) 6. how to fix floating sections? (Caroline Bass) 7. Part-time Lab opportunity in Scottsdale, AZ (Carlos Rodriguez, MD) 8. Paraffin Tissue Crumbles (Michael Mashore) 9. Histo data needed (O'Donnell, Bill) 10. RE: Distance Learning (joelle weaver) 11. Oil Red O trivia (Finlay Finlay) 12. Re: Oil Red O trivia (Geoff McAuliffe) 13. Oil Red O control (Inman, Anna) 14. Sending Slides Out in St. Louis, MO (Alyssa Peterson) 15. Re: Oil Red O control (Drew Meyer) 16. RE: Oil Red O control (Sun,Yuping) 17. Re: Paraffin Tissue Crumbles (Merced M Leiker) 18. RE: Oil Red O control (Weems, Joyce) 19. RE: Paraffin Tissue Crumbles (Nails, Felton) 20. RE: Sending Slides Out in St. Louis, MO (Langenberg, Stacey) 21. Position - Muscle Enzyme Histochem Tech (Barone, Carol ) 22. NH Histology Job Opportunity (Alisha Dynan) 23. New York Dermpath Lab Manager (Alisha Dynan) 24. Laboratory Manager position in MI (Alisha Dynan) 25. Histology Supervisor Job in Oklahoma (Alisha Dynan) 26. Histology Supervisor Position in GA (Alisha Dynan) ---------------------------------------------------------------------- Message: 1 Date: Tue, 9 Nov 2010 13:44:52 -0500 From: Roberta Horner Subject: [Histonet] RE: looking for references to stain protocols To: "Burton, Lynn" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" This is the reference that I have for this stain. Andersen A.A. & VanRompay D (2005). Chlamydiosis. In: A Laboratory Manual for the Isolation and Identification of Avian Pathogens, Fifth Edition. Submitted USA. Roberta Horner HT/HTL Animal Diagnostic La Penn State University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Tuesday, November 09, 2010 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] looking for references to stain protocols I am in need of references for stain protocols. The stain I am searching for in particular is the modified Gimenez, also known as Pierce-Vanderkamp for chlamydia and rickettsia. If anyone can point me in the right direction I would appreciate it. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 9 Nov 2010 14:00:43 -0500 From: Jaclyn_Bhamornsiri@vwr.com Subject: [Histonet] Jaclyn Bhamornsiri - Important Message To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 11/09/2010 and will not return until 12/31/2014. As of Nov 9th, 2010, I am no longer with VWR. If you need assistance, pls contact VWR Customer Service at 1800-932-5000 (or vwrcustomerservice@vwr.com). For more urgent matters, pls contact Leize Lessig at 770-733-8190 (or leize_lessig@vwr.com). Thanks! Jackie B. ------------------------------ Message: 3 Date: Tue, 9 Nov 2010 14:14:48 -0500 From: Lesley Bechtold Subject: [Histonet] Histology Lab Survey To: "histonet@lists.utsouthwestern.edu" Message-ID: <3BDA51FFD1A83B4E90829F594A5C371743316EECB8@JAXBHMAIL01.jax.org> Content-Type: text/plain; charset="us-ascii" Dear Histonetters, Scientific Services at The Jackson Laboratory is conducting benchmarking surveys in an effort to develop an understanding of the current best practices in operations, management and technical delivery at peer institutions. The goal is to gather quality data that can be used to assist us and all survey participants with operational assessment and planning. Survey participants will receive compiled results which will be de-identified before distribution. If you would like to participate, just click on the link below and answer the questions in the survey. Additionally, for those of you who do EM, there is a link to an EM Facility survey as well. It will only take a few minutes of your time. With thanks in advance! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) Histo Survey - http://www.surveymonkey.com/s/5RPSYG2 EM Survey - http://www.surveymonkey.com/s/5RGYH9W ------------------------------ Message: 4 Date: Tue, 09 Nov 2010 12:34:59 -0600 From: "Lin Bustamante" Subject: [Histonet] Cutting and embedding To: Message-ID: <4CD93FF3020000B9000E58DC@CVM.TAMU.EDU> Content-Type: text/plain; charset=US-ASCII Could you please tell me what is the average for embedding surgical blocks in one hour? Is there an average for cutting? Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 ------------------------------ Message: 5 Date: Tue, 9 Nov 2010 13:29:37 -0700 From: "Patsy Ruegg" Subject: [Histonet] NSH Validation WS attendees To: "'ihcrg Group \(E-mail\)'" , , "'National Society of Histotechnology'" Cc: "'Morken, Tim'" , Diane.Tokugawa@kp.org Message-ID: <78B71368E2844D3E9B0E2253896F8291@Patsyoffice> Content-Type: text/plain; charset="us-ascii" For those of you who attended the IHC ws at NSH on Validation by Tim Morken and Patsy Ruegg we have posted the rest of the information we promised you to my ftp://ihctech.net site for you to download. We provided the user name and pass word you will need in the workshop. The folder is labeled "Tim Morken" and you can download the files to your computer. Remember this site is for uploading and downloading not opening. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ Message: 6 Date: Tue, 9 Nov 2010 21:51:02 +0000 From: Caroline Bass Subject: [Histonet] how to fix floating sections? To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="Windows-1252" Hello Everyone, I have a bunch of rat brains that I have frozen in isopentane and stored at -80. I'd like to collect 300 micron sections on a cryostat and collect tissue punches for RNA isolation. I would then like to take the remaining tissue and stain. There are a few ways of doing this, what would work the best is to take two 50 micron section, followed by a 300 micron, followed by two more 50, and go through the brain serially. I will then stain the 50 micron sections with nissl or thionin, so that the structures are easily identified and then use these to direct the tissue punches. The second 50 micron sections would be reserved for GFP IHC. Is this feasible? Here are some direct questions: 1. how would I store the 300 micron sections? 2. Any suggestions/protocols for staining the 50 micron sections for structure? Which stain would you use? 3. Can I post-fix the 50 micron sections? How about the 300 micron? One reason I want to use floating sections for the nissl/thionin is that I can mount these very flat, no wrinkles and I'm very comfortable working with floating sections. I don't want to risk precious tissue trying to optimize this. One methodology recommended to me is to freeze a formalin solution in a 24 well plate, put the section on top flat and let them thaw together at room temperature or 4 degrees. This gives the tissue a nice, slow fixation. Any other suggestions? How about working with the RNA? I'm thinking of using RNAlater-ice for example. Does anyone know if this screws up native fluorescence of GFP? Thanks! Caroline Bass ------------------------------ Message: 7 Date: Tue, 9 Nov 2010 15:47:21 -0700 From: "Carlos Rodriguez, MD" Subject: [Histonet] Part-time Lab opportunity in Scottsdale, AZ To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi all. We have a part-time job opportunity for someone interested primarily in accessioning and grossing skin specimens at our in-house dermatopathology lab (North Scottsdale Dermatology). A small amount of general lab management will also be required (ie, maintaining CLIA logs, ordering supplies etc). The hours needed will likely be Monday, Wednesday and Thursday mornings (~5 or 6am-9am). The start date will be around late December. The hours and start date are flexible, and the workload is very reasonable. Qualifications are either HT certification, or at a minimum an Associate's Degree in a health-related field with college-level credit in Chemistry, Biology, and Math, in addition to work experience in grossing and accessioning skin specimens. If interested, please respond to me by email and include a CV and/or description of your qualifications. Thanks! Carlos Rodriguez, MD ------------------------------ Message: 8 Date: Tue, 9 Nov 2010 14:56:49 -0800 From: "Michael Mashore" Subject: [Histonet] Paraffin Tissue Crumbles To: Message-ID: <652586E049884D8491A0E70448595D40@vandeberg> Content-Type: text/plain; charset="iso-8859-1" Hello Histonet Users, I have just started using paraffin and am having many difficulties. Most of the time my tissue crumbles when sectioning. I have no real experience in paraffin histology and have been given the task of becoming proficient by myself, so I am hoping for feedback as to why my tissue keeps crumbling. The tissue in question has been: skeletal muscle, cardiac muscle, liver, and brain (all from rat). The tissue was fixed in 10% neutral buffered formalin for 7 days at 4?C and then transferred to an automated tissue processor, with the following schedule: 2 hours 70% dehydration alcohol 2 hours 80% dehydration alcohol 2 hours 95% dehydration alcohol 2.5 hours 95% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol .5 hour Hemo-De .5 hour Hemo-De .5 hour Hemo-De 1 hour paraffin 4 hours paraffin They were infiltrated for 1 hour without vacuum then embedded. The blocks were stored in the freezer before cutting. The knife angle was 5?. Sections were 5?m thick. I would appreciate any feedback whatsoever. Thank you very much. Michael ------------------------------ Message: 9 Date: Tue, 9 Nov 2010 16:39:27 -0700 From: "O'Donnell, Bill" Subject: [Histonet] Histo data needed To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Those who work in similar labs please help me out: Could anyone tell me how a clinical histology lab that do 6500 cases a year is staffed. (Average aboout 70 blocks a day, 20-30% small biopsies that require 3 levels so about 120-140 "ribbons" a day) We do not do cyto processing, we have automated H&E.and coverslipping. All special stains and immuno is does manually, but volume is low, 2-4 SS a week and maybe 6 antibodies. We do gross probalbly 60-70% of cases and assist at gross for the rest. We have no aides and are staffed @ 1.5 FTE. I'm trying to justify some help, but the suits need numbers to crunch. I hope that is enough info for comparison. Thanks in advance, Bill 'the tired tech" O'Donnell Kearrney, NE ------------------------------ Message: 10 Date: Wed, 10 Nov 2010 10:47:57 +0000 From: joelle weaver Subject: RE: [Histonet] Distance Learning To: , Histonet , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Phyllis We offer an on line, distance learning program from Columbus State Community Colllege that is fully accredited by NAACLS. It awards a certificate in histotechnology and meets the training and eligibility requirements for the ASCP HT registry examination. The certificate program is linked to an Associates degree in the Department of Allied and Multi-competency Health for those wanting a degree option. We are currently accepting students for the next program year. Our application and admissions process and application form is available from our program web page at www.cscc.edu/histology We are looking to expand our market and meet the needs of students and laboratories outside of Ohio. We have a few clinical affiliates in the Midwest, other than Ohio, and we would like to extend this further into under-served areas, ( as far as histology schools). We are able to offer 24/7 access to materials, instruction in theory and practice, practical work assessment, and interactive delivery via Blackboard learning platform. In the future we plan to offer continuing education opportunities that are histology specific to those aready certified or who may participate in the ASCP- CMP program ,and want to meet the continuing education requirements needed for participation. If I may answer any questions about histology training, certification or the on line study of histology, please feel free to contact me at jweave01@cscc.edu or at 614-287-2217. Joelle Weaver HTL (ASCP) > Date: Mon, 8 Nov 2010 07:26:59 -0800 > From: dchihc@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Distance Learning > > We are thinking of using one of the online or distance learning programs to help > in the training of new histotechs. Has anyone had any experience with any of > these programs, what colleges offer the program, pros, cons. Any feedback is > welcome. > > Thanks > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 10 Nov 2010 14:24:32 -0000 From: "Finlay Finlay" Subject: [Histonet] Oil Red O trivia To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Just wondering if anyone knows the what the 'O' in oil red O stands for. I suspect that it stands for nothing other than O similar to the 'G' in Orange G. One of our pathologists is looking for a tricky stain question to annoy trainees with. Thanks for your help Finlay ------------------------------ Message: 12 Date: Wed, 10 Nov 2010 09:39:10 -0500 From: Geoff McAuliffe Subject: Re: [Histonet] Oil Red O trivia To: Finlay Finlay Cc: histonet@lists.utsouthwestern.edu Message-ID: <4CDAAE8E.20102@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed So, the pathologist does not know the answer but he wants to annoy trainees? Glad I don't work for him! Geoff Finlay Finlay wrote: > Hello > > Just wondering if anyone knows the what the 'O' in oil red O stands for. > I suspect that it stands for nothing other than O similar to the 'G' in > Orange G. One of our pathologists is looking for a tricky stain question > to annoy trainees with. > > Thanks for your help > > Finlay > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 13 Date: Wed, 10 Nov 2010 07:45:28 -0700 From: "Inman, Anna" Subject: [Histonet] Oil Red O control To: "Geoff McAuliffe" , "Finlay Finlay" Cc: histonet@lists.utsouthwestern.edu Message-ID: <2925AE271EAAD440AF48FCCEB8002D091443E583@smgmail01.smgj.sclhs.net> Content-Type: text/plain; charset="us-ascii" Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 14 Date: Wed, 10 Nov 2010 09:51:19 -0500 From: Alyssa Peterson Subject: [Histonet] Sending Slides Out in St. Louis, MO To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, A friend of mine who has a brand new laboratory in St. Louis, MO currently has their histotech prepare their slides but are looking for a laboratory to have them read at because they do not have a Dermatopathologist. Please contact me if you have lab in the area who would be interested in doing that for them. Thank you! -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ------------------------------ Message: 15 Date: Wed, 10 Nov 2010 09:54:46 -0500 From: Drew Meyer <41dmb41@gmail.com> Subject: Re: [Histonet] Oil Red O control To: "Inman, Anna" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 There was a discussion on this recently, so you may want to search the archives for it. In my opinion, the easiest and best control to use is Mayo. Just smear it on a slide as you need it and there you go. Cheap and easy. Drew On Wed, Nov 10, 2010 at 09:45, Inman, Anna wrote: > Hello - > > How does everyone handle having a positive control for Oil Red O - We > very infrequently have this stain and have had difficulty keeping > control on hand. > > > > > > Thank you > > Anna > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 16 Date: Wed, 10 Nov 2010 10:11:41 -0500 From: "Sun,Yuping" Subject: RE: [Histonet] Oil Red O control To: "'Drew Meyer'" <41dmb41@gmail.com>, "'Inman, Anna'" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <530A57D46C74F34B862F95E3F4DFF3228D5D2722@HSC-CMS01.ad.ufl.edu> Content-Type: text/plain; charset="us-ascii" Hello Histonet Users, I have a question about Oil Red O staining. Could the Oil Red O solution(Poly Scientific, Cat.No. S1848) be reused? I would appreciate any feedback whatsoever. Thank you very much. Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Wednesday, November 10, 2010 9:55 AM To: Inman, Anna Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil Red O control There was a discussion on this recently, so you may want to search the archives for it. In my opinion, the easiest and best control to use is Mayo. Just smear it on a slide as you need it and there you go. Cheap and easy. Drew On Wed, Nov 10, 2010 at 09:45, Inman, Anna wrote: > Hello - > > How does everyone handle having a positive control for Oil Red O - We > very infrequently have this stain and have had difficulty keeping > control on hand. > > > > > > Thank you > > Anna > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 10 Nov 2010 10:16:43 -0500 From: Merced M Leiker Subject: Re: [Histonet] Paraffin Tissue Crumbles To: Michael Mashore , histonet@lists.utsouthwestern.edu Message-ID: <449016B996B2D4CDC661E7FC@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=utf-8; format=flowed Having some experience processing and embedding rodent tissues myself (by hand), I would say that you are over-dehydrating the tissues. Try cutting back the alcohol incubation times to 30 min or even 10 min each. Regards, Merced --On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore wrote: > Hello Histonet Users, > > > > I have just started using paraffin and am having many difficulties. Most > of the time my tissue crumbles when sectioning. I have no real experience > in paraffin histology and have been given the task of becoming proficient > by myself, so I am hoping for feedback as to why my tissue keeps > crumbling. The tissue in question has been: skeletal muscle, cardiac > muscle, liver, and brain (all from rat). > > > > The tissue was fixed in 10% neutral buffered formalin for 7 days at 4??C > and then transferred to an automated tissue processor, with the following > schedule: > > > > 2 hours 70% dehydration alcohol > > 2 hours 80% dehydration alcohol > > 2 hours 95% dehydration alcohol > > 2.5 hours 95% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > .5 hour Hemo-De > > .5 hour Hemo-De > > .5 hour Hemo-De > > 1 hour paraffin > > 4 hours paraffin > > > > They were infiltrated for 1 hour without vacuum then embedded. > > The blocks were stored in the freezer before cutting. > > The knife angle was 5??. > > Sections were 5??m thick. > > > > I would appreciate any feedback whatsoever. > > > > Thank you very much. > > > > Michael > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 18 Date: Wed, 10 Nov 2010 10:20:27 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] Oil Red O control To: "Inman, Anna" , Geoff McAuliffe , Finlay Finlay Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044A071B7A@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Smear mayonnaise on a slide.. It works well. And of course - not the fat free kind! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Inman, Anna Sent: Wednesday, November 10, 2010 09:45 To: Geoff McAuliffe; Finlay Finlay Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O control Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 19 Date: Wed, 10 Nov 2010 09:25:47 -0600 From: "Nails, Felton" Subject: RE: [Histonet] Paraffin Tissue Crumbles To: "'Merced M Leiker'" , "Michael Mashore" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 I agree most of your times are too long, but you can still get sections if you put water on your ice block and allow the tissue to rehydrate a bit or put a little ammonium hydroxide and water on your ice and sit your blocks to be section in this solution. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Wednesday, November 10, 2010 9:17 AM To: Michael Mashore; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Tissue Crumbles Having some experience processing and embedding rodent tissues myself (by hand), I would say that you are over-dehydrating the tissues. Try cutting back the alcohol incubation times to 30 min or even 10 min each. Regards, Merced --On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore wrote: > Hello Histonet Users, > > > > I have just started using paraffin and am having many difficulties. > Most of the time my tissue crumbles when sectioning. I have no real > experience in paraffin histology and have been given the task of > becoming proficient by myself, so I am hoping for feedback as to why > my tissue keeps crumbling. The tissue in question has been: skeletal > muscle, cardiac muscle, liver, and brain (all from rat). > > > > The tissue was fixed in 10% neutral buffered formalin for 7 days at > 4?C and then transferred to an automated tissue processor, with the > following > schedule: > > > > 2 hours 70% dehydration alcohol > > 2 hours 80% dehydration alcohol > > 2 hours 95% dehydration alcohol > > 2.5 hours 95% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > .5 hour Hemo-De > > .5 hour Hemo-De > > .5 hour Hemo-De > > 1 hour paraffin > > 4 hours paraffin > > > > They were infiltrated for 1 hour without vacuum then embedded. > > The blocks were stored in the freezer before cutting. > > The knife angle was 5?. > > Sections were 5?m thick. > > > > I would appreciate any feedback whatsoever. > > > > Thank you very much. > > > > Michael > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== ------------------------------ Message: 20 Date: Wed, 10 Nov 2010 08:30:22 -0700 From: "Langenberg, Stacey" Subject: RE: [Histonet] Sending Slides Out in St. Louis, MO To: Alyssa Peterson , "histonet@lists.utsouthwestern.edu" Message-ID: <1F70FCBB6D4EC549B2ADF69B9F9EAC0348F696A29E@STEAMBOAT.ucdenver.pvt> Content-Type: text/plain; charset="us-ascii" Hi Alyssa, We are not in St Louis but I work for a really great Dermpath here in Colorado who might read them for you. "People are not an interruption of our business. People are our business." Stacey Langenberg HT (ASCP) QIHC Laboratory Manager Histology/IF CU Dermatopathology Consultants 1999 N. Fitzsimons Pkwy Suite 120 Aurora, CO 80045 Lab-720-859-3559 Fax- 303-344-0789 Office- 303-577-2303 Cell-970-405-7742 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson [alyssa@alliedsearchpartners.com] Sent: Wednesday, November 10, 2010 7:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sending Slides Out in St. Louis, MO Hello, A friend of mine who has a brand new laboratory in St. Louis, MO currently has their histotech prepare their slides but are looking for a laboratory to have them read at because they do not have a Dermatopathologist. Please contact me if you have lab in the area who would be interested in doing that for them. Thank you! -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 10 Nov 2010 11:00:57 -0500 From: "Barone, Carol " Subject: [Histonet] Position - Muscle Enzyme Histochem Tech To: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A81A3@wlmmsx01.nemours.org> Content-Type: text/plain; charset="us-ascii" Hello Histonetters: We presently have a position available for a HT/HTL (certified, or eligible) in our clinical muscle enzyme histochemistry lab (within the Department of Biomedical Research). We request 3 yrs experience minimum in EHC... and skills for manual IF, as well. If you meet these requirements and have interest, please contact cbarone@nemours.org. We are looking for you! ------------------------------ Message: 22 Date: 10 Nov 2010 11:55:24 -0500 From: Alisha Dynan Subject: [Histonet] NH Histology Job Opportunity To: histonet@lists.utsouthwestern.edu Message-ID: <607630384.1289408124244.JavaMail.cfservice@SL4APP4> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am currently working on a Histology Supervisor and Histology Technologist job opportunity in Southern NH. These job opportunities are with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salray and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 23 Date: 10 Nov 2010 11:57:53 -0500 From: Alisha Dynan Subject: [Histonet] New York Dermpath Lab Manager To: histonet@lists.utsouthwestern.edu Message-ID: <777306831.1289408273737.JavaMail.cfservice@SL4APP4> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am currently working on a Laboratory Manager position in NY. the ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in dermatopathology. This job opportunity is with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 24 Date: 10 Nov 2010 11:58:16 -0500 From: Alisha Dynan Subject: [Histonet] Laboratory Manager position in MI To: histonet@lists.utsouthwestern.edu Message-ID: <1327844936.1289408296253.JavaMail.cfservice@SL4APP1> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am currently working on a Laboratory Manager position in MIY. the ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in dermatopathology. This job opportunity is with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 25 Date: 10 Nov 2010 11:59:26 -0500 From: Alisha Dynan Subject: [Histonet] Histology Supervisor Job in Oklahoma To: histonet@lists.utsouthwestern.edu Message-ID: <768330664.1289408366500.JavaMail.cfservice@SL4APP1> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am currently working on a Histology Supervisor position in OK. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 26 Date: 10 Nov 2010 12:00:45 -0500 From: Alisha Dynan Subject: [Histonet] Histology Supervisor Position in GA To: histonet@lists.utsouthwestern.edu Message-ID: <2144143006.1289408445189.JavaMail.cfservice@sl4app2> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am currently working on a Histology Supervisor position in GA. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. The candidate must also be interested/willing to live in a rural area but work in a large teaching hospital (600+ beds). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 84, Issue 11 **************************************** Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From higginst <@t> amapath.com Wed Nov 10 14:38:54 2010 From: higginst <@t> amapath.com (Tim Higgins) Date: Wed Nov 10 14:44:24 2010 Subject: [Histonet] (no subject) Message-ID: <000001cb8117$4d3dfc90$6a03a8c0@apg> Hi Histonetters, This maybe a stupid question but I'm going to ask anyways. Is it legal to buy tissue blocks (with human tissue) from another lab to use as controls for specials and Immunos? I know we purchase slides from vendors to use with our specials and Immunos. Thanks! Tim From macveigh <@t> usc.edu Wed Nov 10 14:57:44 2010 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Wed Nov 10 14:57:42 2010 Subject: [Histonet] Oil Red O control Message-ID: <004d01cb8119$ebd6f5d0$c384e170$@usc.edu> Once you mix your stock solution with water, it is good for only about an hour. Then you have to discard it. Michelle Aloni MS HTL (ASCP) USC Keck School of Medicine From Rcartun <@t> harthosp.org Wed Nov 10 15:26:30 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Nov 10 15:26:41 2010 Subject: [Histonet] HSP-90 (beta) Message-ID: <4CDAC7B6.7400.0077.1@harthosp.org> Does anyone have experience with an antibody to Heat shock protein-90 (beta) for IHC? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From smcbride <@t> andrew.cmu.edu Wed Nov 10 15:36:34 2010 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Wed Nov 10 15:36:40 2010 Subject: [Histonet] Correcting van Gieson over-stain Message-ID: Hi folks, We have a set of slides that we stained in van Gieson's solution, and unfortunately, they turned out too dark. I tried immersion in 100% ethanol for ~2 minutes, and that helped a little, but they are still too dark. Does anyone have any experience or suggestions as to what solution to use to better differentiate the staining? Thanks in advance for all of your suggestions! ~Sean From cbarone <@t> NEMOURS.ORG Wed Nov 10 15:48:37 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Nov 10 15:48:40 2010 Subject: [Histonet] Position available Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A81AA@wlmmsx01.nemours.org> Hello Histonetters: We presently have a position available for a HT/HTL (certified, or eligible) in our clinical muscle enzyme histochemistry lab (within the Department of Biomedical Research). We request 3 yrs experience minimum in EHC... and skills for manual IF, as well. If you meet these requirements and have interest, please contact cbarone@nemours.org . We are looking for you! Check us out on line at www.nemours.org We are 25 minutes south of Philadelphia, on a private campus, free parking and no state sales tax! Nemours - A.I. duPont Hospital for Children Department of Biomedical Research Histotechnology Core Lab Wilmington, DE 19803 From mmashore <@t> vapop.ucsd.edu Wed Nov 10 16:07:43 2010 From: mmashore <@t> vapop.ucsd.edu (Michael Mashore) Date: Wed Nov 10 16:13:00 2010 Subject: [Histonet] RE: Paraffin Tissue Crumbles Message-ID: <119281F8227345FAB4BB9843C05614B3@vandeberg> Hello to everyone, Thank you for all the advice. I will reduce my processing time as well as try to rehyrdate my paraffin blocks before cutting. I greatly appreciate the help. Thanks, Michael From galinadeyneko <@t> yahoo.com Wed Nov 10 16:52:10 2010 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Wed Nov 10 16:52:16 2010 Subject: [Histonet] Re: Parrafin tissues crumbles Message-ID: <975501.60416.qm@web33103.mail.mud.yahoo.com> Hi Michael. 1. Fixation : 48 hours at room T is a good time to fix a big rat heart.?You can transfer at 4C after 24/48 hours, otherwise cold T from the beginning prevents good penetration and fixation. 2.. process the hearts and muscles separate from other tissues 3. use only fresh reagents in you processing machine. I always rotate reagents before process the hearts? and this really works for me.2 hours in each Ethanols is OK in my opinion but according me experience it depend on processing center .I think that you use xylene substitute and I can recommend to switch back to real Xylene's. the clearing in Xylene is very short -30 Minutes?, especially after long dehydration. It would be better if you use 1.30 or 2 hours on each. Time in paraffin is OK, but I prefer 3 baths of paraffin, 1.30 or 2 hours in each. In the processing center use paraffin called infiltration medium i use from surgipath # 01400, for embedding i highly recommend Shandon Precision Cut paraffin from Thermo Shandon# B1002490. Do?not freeze paraffin blocks this make the paraffin and tissues very fragile. You can cool the blocks in refrigerator or on the top of cold plate. Trim the paraffin block and place in the DI water for soaking for 5-6 minutes, do not keep to long. Use only DI water in the floating bath. Moisten the cutting surface of the block with your finger during sectioning, this is help to get more smooth sections. My English is not perfect but I hope you understand my tips.please contact with me if you have additional questions. Galina Deyneko Novartis, Cambridge, MA 617-871-7613 galinadeyneko@yahoo.com ? Message: 8 Date: Tue, 9 Nov 2010 14:56:49 -0800 From: "Michael Mashore" Subject: [Histonet] Paraffin Tissue Crumbles To: Message-ID: <652586E049884D8491A0E70448595D40@vandeberg> Content-Type: text/plain;??? charset="iso-8859-1" Hello Histonet Users, I have just started using paraffin and am having many difficulties. Most of the time my tissue crumbles when sectioning. I have no real experience in paraffin histology and have been given the task of becoming proficient by myself, so I am hoping for feedback as to why my tissue keeps crumbling. The tissue in question has been:? skeletal muscle, cardiac muscle, liver, and brain (all from rat). The tissue was fixed in 10% neutral buffered formalin for 7 days at 4?C and then transferred to an automated tissue processor, with the following schedule: 2 hours 70% dehydration alcohol 2 hours 80% dehydration alcohol 2 hours 95% dehydration alcohol 2.5 hours 95% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol .5 hour Hemo-De .5 hour Hemo-De .5 hour Hemo-De 1 hour paraffin 4 hours paraffin They were infiltrated for 1 hour without vacuum then embedded. The blocks were stored in the freezer before cutting. The knife angle was 5?. Sections were 5?m thick. I would appreciate any feedback whatsoever. Thank you very much. Michael ------------------------------ From lpwenk <@t> sbcglobal.net Wed Nov 10 19:08:17 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Nov 10 19:08:20 2010 Subject: [Histonet] Oil Red O control In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D091443E583@smgmail01.smgj.sclhs.net> References: <4CDAAE8E.20102@umdnj.edu> <2925AE271EAAD440AF48FCCEB8002D091443E583@smgmail01.smgj.sclhs.net> Message-ID: <33B9F7F300D84F6DB40B0076F5A8C43C@HP2010> Cut frozen section of adrenal from an autopsy or surgical pathology. Store slides in a box in the refrig. Good for a year. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Inman, Anna" Sent: Wednesday, November 10, 2010 9:45 AM To: "Geoff McAuliffe" ; "Finlay Finlay" Cc: Subject: [Histonet] Oil Red O control > Hello - > > How does everyone handle having a positive control for Oil Red O - We > very infrequently have this stain and have had difficulty keeping > control on hand. > > > > > > Thank you > > Anna > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Nov 10 17:20:02 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Nov 10 21:56:58 2010 Subject: [Histonet] ThinPrep vs. SurePath Message-ID: Does anyone have an opinion as to why there seems to be many more ThinPreps out there than Sure Path? The SurePath seems more efficient, but I might be missing something. Thank you, Jennifer MacDonald From f.finlay <@t> formed.gla.ac.uk Thu Nov 11 02:55:56 2010 From: f.finlay <@t> formed.gla.ac.uk (Finlay Finlay) Date: Thu Nov 11 02:56:04 2010 Subject: [Histonet] ORO trivia Message-ID: Thanks to everyone who replied. No firm answer. I've told my pathologist that I don't know and may now suggest to one of the trainees that they ask him. Finlay From talulahgosh <@t> gmail.com Thu Nov 11 09:26:25 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Nov 11 09:26:31 2010 Subject: [Histonet] VERY OT but perhaps histological Message-ID: I thought this would be more interesting to ask people who are working in labs: The item to the left of you is now your weapon in the impending zombie apocalypse. What is it? Sorry about the OT, but I did label it. And hey, we call all learn how histology helps kill zombies. Or how your mug of coffee will make you feel better before the zombies kill you. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx From CBraaten <@t> Cheshire-Med.COM Thu Nov 11 09:52:56 2010 From: CBraaten <@t> Cheshire-Med.COM (Braaten, Christine I) Date: Thu Nov 11 09:53:03 2010 Subject: [Histonet] lost tissue Message-ID: Hi everyone, Has anyone had a problem with cassettes opening during processing? Sometimes the cassettes are slightly opened at the top or bottom. We rarely lose a specimen and it's frustrating to cause the patient to be biopsied again with no explanation what happened to it. We use Surgipath micro cassettes and a Peloris processor and just in the past three or four months we have lost 2 specimens somewhere between grossing and embedding. The specimens are tiny skin biopsies and have no paper or biopsy pads in the cassette with them. They are not small enough to fit through the cassette "holes". If anyone has any ideas what might be happening I would appreciate a response. I have been embedding for 8 years and it's just been in the recent past that this has happened. Thanks, Christine CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From mpence <@t> grhs.net Thu Nov 11 10:07:39 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Nov 11 10:07:44 2010 Subject: [Histonet] lost tissue In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974A93@is-e2k3.grhs.net> Are you sure the cassette is opening during processing? I have seen many time when small specimens are loose in the cassette the specimen flips up when the lid is opened and it then falls somewhere else. I have had this happen to me and my techs more than once. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Braaten, Christine I Sent: Thursday, November 11, 2010 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lost tissue Hi everyone, Has anyone had a problem with cassettes opening during processing? Sometimes the cassettes are slightly opened at the top or bottom. We rarely lose a specimen and it's frustrating to cause the patient to be biopsied again with no explanation what happened to it. We use Surgipath micro cassettes and a Peloris processor and just in the past three or four months we have lost 2 specimens somewhere between grossing and embedding. The specimens are tiny skin biopsies and have no paper or biopsy pads in the cassette with them. They are not small enough to fit through the cassette "holes". If anyone has any ideas what might be happening I would appreciate a response. I have been embedding for 8 years and it's just been in the recent past that this has happened. Thanks, Christine CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brandihiggins <@t> gmail.com Thu Nov 11 10:18:33 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Thu Nov 11 10:18:38 2010 Subject: [Histonet] lost tissue In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974A93@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974A93@is-e2k3.grhs.net> Message-ID: Hi Christine, We recently had a cassette that when we picked it up to embed we could see that it was open. There was nothing in the cassette, we later found the specimen in the retort of the processing machine. When I tried to close the lid of that cassette (microbiopsy cassette, and we also use Surgipath) the lid was particularly difficult to close. Probably the grosser pressed and thought it had shut, but it had not closed propoerly and it opened more during the agitation or the solutions being pumped in or out and the tissue floated out. But, as I mentioned, the cassette was open when we picked it up. If the cassette is closed when you pick it up to embed, I don't think it would have opened and shut itself properly again. Brandi Higgins On Thu, Nov 11, 2010 at 11:07 AM, Mike Pence wrote: > Are you sure the cassette is opening during processing? I have seen > many time when small specimens are loose in the cassette the specimen > flips up when the lid is opened and it then falls somewhere else. I > have had this happen to me and my techs more than once. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Braaten, > Christine I > Sent: Thursday, November 11, 2010 9:53 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] lost tissue > > > Hi everyone, > > > > Has anyone had a problem with cassettes opening during processing? > > Sometimes the cassettes are slightly opened at the top or bottom. We > > rarely lose a specimen and it's frustrating to cause the patient to be > > biopsied again with no explanation what happened to it. We use Surgipath > > micro cassettes and a Peloris processor and just in the past three or > > four months we have lost 2 specimens somewhere between grossing and > > embedding. The specimens are tiny skin biopsies and have no paper or > > biopsy pads in the cassette with them. They are not small enough to fit > > through the cassette "holes". If anyone has any ideas what might be > > happening I would appreciate a response. I have been embedding for 8 > > years and it's just been in the recent past that this has happened. > > Thanks, Christine > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brandihiggins <@t> gmail.com Thu Nov 11 10:22:44 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Thu Nov 11 10:22:49 2010 Subject: [Histonet] xylene Message-ID: Good Morning Histonet! We use xylene substitute for almost everything, but I still find that we need to keep some xylene on hand for certain tasks, especially removal of coverslips. We use Formula 83 for everything else, but I have let slides soak in Formula 83 in the past for a multiple days and still couldn't remove the coverslip. Are there any labs out there that are completely xylene free, and if so, what substitute are you using and are you able to remove coverslips with the substitute? My employer would like us to be 100% xylene free, but I feel we need the xylene for these few tasks. Thanks in advance for you help. Brandi Higgins From alisha <@t> ka-recruiting.com Thu Nov 11 10:31:40 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Nov 11 10:31:12 2010 Subject: [Histonet] General Manager of AP Operations Job in NC Message-ID: <545718765.1289493100249.JavaMail.cfservice@sl4app2> Hi Histonet Members, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few positions across the country. Our clients typically assist with relocation expenses. One particular client I am working with is looking for a General Manager of Anatomic Pathology Operations for an anatomic pathology and clinical lab in North Carolina. This laboratory is known for its cutting edge technology, LIS system, and its automation. They are looking for someone with 10-20+ years experience in histology/pathology, preferably an HTL(ASCP) certification, at least 10+ years experience in management/operations, and someone who is a strong leader with excellent communication skills. They will also look at someone with a very strong background in clinical laboratory operations. This is a high level position, managing a 160 person laboratory and offers an excellent compensation and benefits package. The ideal candidate must be a free, independent and strategic thinker. If interested, please email me your resume to alisha@ka-recruiting.com. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From sbreeden <@t> nmda.nmsu.edu Thu Nov 11 10:34:01 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Nov 11 10:34:06 2010 Subject: [Histonet] lost tissue In-Reply-To: References: <661949901A768E4F9CC16D8AF8F2838C03974A93@is-e2k3.grhs.net> Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47451@nmdamailsvr.nmda.ad.nmsu.edu> Gremlins. That's what it is. I recently cut in a case with two parts - one Extremely Thin Black Crescent of tissue in one cassette and the other tissue in the next cassette. The next morning, the Extremely Thin Black Crescent was NOT in the cassette. Gulp. Sometime later, as I was almost done embedding, I opened a cassette and there - lo and behold! - was the Extremely Thin Black Crescent. Creepy. I had the pathologist confirm that it was, indeed, the Extremely Thin Black Crescent of tissue that had started out somewhere else... 'splain that, Lucy! From brandihiggins <@t> gmail.com Thu Nov 11 11:10:56 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Thu Nov 11 11:11:00 2010 Subject: [Histonet] xylene In-Reply-To: References: Message-ID: My responses to some questions I have received (also FYI for anyone else with the same issue): Are you using the tape coverslips or the glass ones? If using the tape, it will only be removed with acetone. See what your mounting medium is based with (Xylene? Toluene? etc.,) and see if Formula 83 is compatible - that may be the issue. We use glass and coverslip by hand (if that matters). Mounting medium is toluene based. We are completely xylene free and we use an aliphatic xylene substitute from VWR (catalog # 95057-820)...We use the Ventana Symphony for staining and coverslipping and those coverslips we just place on a hot plate for a minute or so and the coverslip slides right off...But we used to use a tape coverslipper and the xylene sub removed those coverslips just fine! Hope that helps! What temp is the hot plate? I have put slides in the oven at 65 for a while and haven't gotten the coverslip off. We don't have a hot plate. Thanks, Brandi Higgins From: Brandi Higgins To: histonet@lists.utsouthwestern.edu Date: 11/11/2010 10:26 AM Subject: [Histonet] xylene ------------------------------ From Joyce.Cline <@t> wchsys.org Thu Nov 11 11:45:28 2010 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Thu Nov 11 11:51:20 2010 Subject: [Histonet] xylene In-Reply-To: References: Message-ID: We use Formula 83 and we still have to use Xyene to remove coverslips from slides that are more than 2 days old. Joyce Cline Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@wchsys.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins [brandihiggins@gmail.com] Sent: Thursday, November 11, 2010 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] xylene Good Morning Histonet! We use xylene substitute for almost everything, but I still find that we need to keep some xylene on hand for certain tasks, especially removal of coverslips. We use Formula 83 for everything else, but I have let slides soak in Formula 83 in the past for a multiple days and still couldn't remove the coverslip. Are there any labs out there that are completely xylene free, and if so, what substitute are you using and are you able to remove coverslips with the substitute? My employer would like us to be 100% xylene free, but I feel we need the xylene for these few tasks. Thanks in advance for you help. Brandi Higgins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Jackie.Fleming <@t> allina.com Thu Nov 11 11:58:14 2010 From: Jackie.Fleming <@t> allina.com (Fleming, Jackie M) Date: Thu Nov 11 11:58:19 2010 Subject: [Histonet] 9 hour tissue processing Message-ID: Does anyone use a 9 hour tissue processing that is actually 9 hours. Ours is 10 with the pumpin and out.Thanks! Jackie Fleming HT ASCP Technical Consultant - Histology Phone: 612-863- 4773 Pager: 612-654-2135 e-mail: jackie.fleming@allina.com This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. From brandihiggins <@t> gmail.com Thu Nov 11 11:59:41 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Thu Nov 11 11:59:45 2010 Subject: [Histonet] xylene In-Reply-To: References: Message-ID: glass coverslips On Thu, Nov 11, 2010 at 12:50 PM, Ranostaj, Drew wrote: > Are you coverslips tape or glass that you are trying to remove? > > Drew Ranostaj > Medite Inc. > 1226 Winter Garden Vineland Rd, Suite 104 > Winter Garden, Florida 34787, U.S.A. > > Phone: +1 (407) 996 9630 > Fax: +1 (407) 996 9631 > Toll Free Phone: 888 225 2950 > http://www.medite-group.com > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Brandi Higgins > Gesendet: Donnerstag, 11. November 2010 17:23 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] xylene > > Good Morning Histonet! > > We use xylene substitute for almost everything, but I still find that we > need to keep some xylene on hand for certain tasks, especially removal of > coverslips. We use Formula 83 for everything else, but I have let slides > soak in Formula 83 in the past for a multiple days and still couldn't > remove > the coverslip. Are there any labs out there that are completely xylene > free, and if so, what substitute are you using and are you able to remove > coverslips with the substitute? My employer would like us to be 100% > xylene > free, but I feel we need the xylene for these few tasks. Thanks in advance > for you help. > > Brandi Higgins > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Thu Nov 11 12:50:12 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Nov 11 12:50:19 2010 Subject: [Histonet] Ventana Symphony Message-ID: <80F510CD-DD13-41A7-8CF0-04C5D35AFFFE@yahoo.com> Hi all of you in Histoland! I want to recognize all of you who have served in the Armed Forces. I am an old Marine Corp Brat, so Semper Fi and Thank-you! Thank-you! Thank-you! You gave us years of your lives and took risks on behalf of your country. I admire you..... Now to the question at hand... I would like any and ALL of your comments regarding the "Ventana Symphony". I just started working with a laboratory that is considering purchasing a Ventana Symphony after demo'ing it. There is going to be a meeting in a couple of weeks, and I want to be fully prepared with the pro's and con's regarding this instrument. I want to keep an open mind, but I have had absolutely NO experience with this instrument. My 40 plus years in the field has been with the use of manuel H&E staining, and the Leica and Sakura stainers. The Leica and Sakura Stainers have a solid track record, and are the recognized leaders in the field. I have been informed by the AP manager, who is a (PA), that the Ventana Symphony is Green, and that was a very attractive feature for her. Right off the bat, I recognized the following issues: Cost of instrument and solutions Proven Track Record? Huge Footprint Proprietary Solutions? How can I troubleshoot any issues that may arise??? Also, how do I know these solutions are "GREEN" if the ingredients are not disclosed? California is extremely rigid to disposal of ALL solutions! Having worked in both the Clinical and Biotech arenas, I can see both sides to the issue of "Proprietary Ingredients". Biotech companies do not want to disclose the ingredients because they do not want to lose the edge on sales. I developed numerous products with formulas that stated "Proprietary Ingredients" because I was told by my CEO to omit certain ingredients and state "Proprietary Ingredient" . The end-user becomes reliant on your expertise for troubleshooting, thus creating sales.... Also, these companies do not want the competition to know the ingredients because they can duplicate the product. Trust me, I used to reverse engineer numerous products from our competition and create a new product for sale! I realize it is extremely difficult to break into the field when you are introducing a new product or instrument. Ventana tried to introduce a Tissue Processor back in the late 90's early 2000 with little success. Perhaps, Ventana has a great instrument with the Symphony. I would appreciate your comments. Thank you in advance for your input! Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From jaylundgren <@t> gmail.com Thu Nov 11 14:37:56 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Nov 11 14:38:04 2010 Subject: [Histonet] Ventana Symphony In-Reply-To: <80F510CD-DD13-41A7-8CF0-04C5D35AFFFE@yahoo.com> References: <80F510CD-DD13-41A7-8CF0-04C5D35AFFFE@yahoo.com> Message-ID: Akemi, Be careful what vendors tell you about being green. As previously discussed in this forum, Ventana's solution to the disposal of DAB from their immunostainers is to pay to have the wast hauled off. Jay A. Lundgren M.S., HTL (ASCP) From dgmiller <@t> coho.net Thu Nov 11 15:01:19 2010 From: dgmiller <@t> coho.net (Diane G Miller) Date: Thu Nov 11 15:05:53 2010 Subject: [Histonet] Looking for Ellen Sabato, please contact me Message-ID: Hi, I'd like to talk with Ellen. If someone knows where to contact her, please tell her that Diane Miller would like to talk with her. My email address is dgmiller@coho.net and my phone number is 503-784-6444. Thanks for your help. Best Regards, Diane From michaels <@t> janelia.hhmi.org Thu Nov 11 15:18:04 2010 From: michaels <@t> janelia.hhmi.org (Michael, Susan) Date: Thu Nov 11 15:18:10 2010 Subject: [Histonet] Vibratome sectioning of mouse spinal cord Message-ID: Can anyone give me some tips on sectioning mouse spinal cord on the vibratome? Specifically, once the dura is successfully removed, I have been sectioning 40 um horizontal sections, and some cords have been curling up on themselves, both rostral-caudal and medial-lateral, and are impossible to uncurl without destroying the tissue. I?ve tried every angle of approach, speed, amplitude, the tissue has been in sucrose, embedded in agarose, and chilled. Thanks, Susan From Vickroy.Jim <@t> mhsil.com Thu Nov 11 16:29:43 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu Nov 11 16:29:57 2010 Subject: [Histonet] CHANGING PROCESSOR CHEMISTRY Message-ID: <24A4826E8EF0964D86BC5317306F58A554FFB60E66@mmc-mail.ad.mhsil.com> Like everyone we have had a rare occasion when tissue processed did not come out well at all. My experience over the years has led me to believe that when there is an entire machine full of poorly processed tissue that the problem is usually human error where probably one of the chemicals was mixed or put into the wrong container. There are of course situations where processors have failed but more likely the error was in loading the processor. With that said can any of you share procedural changes you have added to try to prevent the problem. We have made some immediate small changes and want to see if anyone else has any better ideas. Thanks for your input. Of course we did reprocess the tissue however we all know it never turns out great. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From godsgalnow <@t> aol.com Thu Nov 11 16:44:52 2010 From: godsgalnow <@t> aol.com (=?utf-8?B?Z29kc2dhbG5vd0Bhb2wuY29t?=) Date: Thu Nov 11 16:44:43 2010 Subject: [Histonet] Formalin Message-ID: <201011112244.oABMica0029530@imr-ma03.mx.aol.com> Ok, so how many of you are allowed to dump formalin down the drain? Sent from my Verizon Wireless Phone From lori.garcia <@t> medtronic.com Thu Nov 11 17:29:51 2010 From: lori.garcia <@t> medtronic.com (Garcia, Lori, Sr. Scientist) Date: Thu Nov 11 17:29:57 2010 Subject: [Histonet] Formalin In-Reply-To: <201011112244.oABMica0029530@imr-ma03.mx.aol.com> References: <201011112244.oABMica0029530@imr-ma03.mx.aol.com> Message-ID: <5A8A2A45BE610D459A95CD6A9102102A011144E5E2@STSM1BMSGM04.ent.core.medtronic.com> The labs I worked for in Missouri all dumped down the drain. Now I'm in California and can't even put ETOH in the pipes. I'm hoping they have changed practices in MO in the last few years, but doubt it. Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, November 11, 2010 2:45 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Ok, so how many of you are allowed to dump formalin down the drain? Sent from my Verizon Wireless Phone [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From deannal78 <@t> verizon.net Thu Nov 11 17:48:16 2010 From: deannal78 <@t> verizon.net (Deanna Rhoads) Date: Thu Nov 11 17:48:20 2010 Subject: [Histonet] Formalin In-Reply-To: <5A8A2A45BE610D459A95CD6A9102102A011144E5E2@STSM1BMSGM04.ent.core.medtronic.com> References: <201011112244.oABMica0029530@imr-ma03.mx.aol.com> <5A8A2A45BE610D459A95CD6A9102102A011144E5E2@STSM1BMSGM04.ent.core.medtronic.com> Message-ID: <760169.1005.qm@web84307.mail.re1.yahoo.com> I'm in Pittsburgh and we aren't allowed to put formalin or ETOH down the drain.? Deanna ________________________________ From: "Garcia, Lori, Sr. Scientist" To: "godsgalnow@aol.com" ; "Histonet@lists.utsouthwestern.edu" Sent: Thu, November 11, 2010 6:29:51 PM Subject: RE: [Histonet] Formalin The labs I worked for in Missouri all dumped down the drain. Now I'm in California and can't even put ETOH in the pipes. I'm hoping they have changed practices in MO in the last few years, but doubt it. Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, November 11, 2010 2:45 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Ok, so how many of you are allowed to dump formalin down the drain? Sent from my Verizon Wireless Phone [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Nov 11 18:11:05 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Nov 11 18:12:15 2010 Subject: [Histonet] Formalin In-Reply-To: <760169.1005.qm@web84307.mail.re1.yahoo.com> References: <201011112244.oABMica0029530@imr-ma03.mx.aol.com> <5A8A2A45BE610D459A95CD6A9102102A011144E5E2@STSM1BMSGM04.ent.core.medtronic.com>, <760169.1005.qm@web84307.mail.re1.yahoo.com> Message-ID: You have to get permission to dump it from you local water people.....it varies by location/state laws etc. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deanna Rhoads [deannal78@verizon.net] Sent: Thursday, November 11, 2010 6:48 PM To: Garcia, Lori, Sr. Scientist; godsgalnow@aol.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin I'm in Pittsburgh and we aren't allowed to put formalin or ETOH down the drain. Deanna ________________________________ From: "Garcia, Lori, Sr. Scientist" To: "godsgalnow@aol.com" ; "Histonet@lists.utsouthwestern.edu" Sent: Thu, November 11, 2010 6:29:51 PM Subject: RE: [Histonet] Formalin The labs I worked for in Missouri all dumped down the drain. Now I'm in California and can't even put ETOH in the pipes. I'm hoping they have changed practices in MO in the last few years, but doubt it. Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, November 11, 2010 2:45 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Ok, so how many of you are allowed to dump formalin down the drain? Sent from my Verizon Wireless Phone [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Thu Nov 11 18:25:27 2010 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Thu Nov 11 18:26:04 2010 Subject: [Histonet] lost tissue In-Reply-To: References: Message-ID: <4CDD1617.471C.0039.0@health.qld.gov.au> Hi Christine You have had some good suggestions to date. Also to consider if you are using the Peloris racks with the individual spaces for cassettes I have seen this happen when cassettes are inserted into the racks. As most people load cassettes with the patient details facing up, if not careful the lid can make contact with the cassette divider and open the lid. We had this happen in my lab and now the formalin that contains the racks prior to processing is poured through a sieve before disposal. We have saved one specimen with this process and even if it is only 1 in a million it is worth it. As the tissue is not wrapped also consider that tissue will shrink during processing. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Braaten, Christine I" 12/11/2010 1:52 am >>> Hi everyone, Has anyone had a problem with cassettes opening during processing? Sometimes the cassettes are slightly opened at the top or bottom. We rarely lose a specimen and it's frustrating to cause the patient to be biopsied again with no explanation what happened to it. We use Surgipath micro cassettes and a Peloris processor and just in the past three or four months we have lost 2 specimens somewhere between grossing and embedding. The specimens are tiny skin biopsies and have no paper or biopsy pads in the cassette with them. They are not small enough to fit through the cassette "holes". If anyone has any ideas what might be happening I would appreciate a response. I have been embedding for 8 years and it's just been in the recent past that this has happened. Thanks, Christine CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From jmacdonald <@t> mtsac.edu Thu Nov 11 18:45:36 2010 From: jmacdonald <@t> mtsac.edu (jmacdonald@mtsac.edu) Date: Thu Nov 11 18:45:41 2010 Subject: [Histonet] Formalin In-Reply-To: <201011112244.oABMica0029530@imr-ma03.mx.aol.com> References: <201011112244.oABMica0029530@imr-ma03.mx.aol.com> Message-ID: <929240319-1289522732-cardhu_decombobulator_blackberry.rim.net-89595305-@bda551.bisx.prod.on.blackberry> Absolutely not here in southern California. Sent via BlackBerry by AT&T -----Original Message----- From: "godsgalnow@aol.com" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Thu, 11 Nov 2010 17:44:52 To: Subject: [Histonet] Formalin Ok, so how many of you are allowed to dump formalin down the drain? Sent from my Verizon Wireless Phone From histotech <@t> imagesbyhopper.com Thu Nov 11 20:36:43 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Nov 11 20:37:23 2010 Subject: [Histonet] Formalin In-Reply-To: <929240319-1289522732-cardhu_decombobulator_blackberry.rim.net-89595305-@bda551.bisx.prod.on.blackberry> References: <201011112244.oABMica0029530@imr-ma03.mx.aol.com> <929240319-1289522732-cardhu_decombobulator_blackberry.rim.net-89595305-@bda551.bisx.prod.on.blackberry> Message-ID: <2C3A3222-C26B-4AB8-ACCB-748F9660E270@imagesbyhopper.com> Not in Florida either. We use Aldex to neutralize the formalin & then dispose of it on the biohazard trash. The mfr says once neutralized you can put it in regular trash, but we don't. Michelle On Nov 11, 2010, at 7:45 PM, jmacdonald@mtsac.edu wrote: > Absolutely not here in southern California. > > > Sent via BlackBerry by AT&T > > -----Original Message----- > From: "godsgalnow@aol.com" > Sender: histonet-bounces@lists.utsouthwestern.edu > Date: Thu, 11 Nov 2010 17:44:52 > To: > Subject: [Histonet] Formalin > > Ok, so how many of you are allowed to dump formalin down the drain? > > Sent from my Verizon Wireless Phone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dhill32 <@t> comcast.net Thu Nov 11 20:51:00 2010 From: dhill32 <@t> comcast.net (David Hill) Date: Thu Nov 11 20:51:03 2010 Subject: [Histonet] CK7/EMA control tissue Message-ID: I'm looking to find some control tissue that will stain positive for ck7 and ema. We are currently using breast carcinoma tissue but most of the tumors we are seeing upon grossing are being submitted entirely for pathologist review. I have been searching the literature for any normal tissue that stains for ck7 and ema with no luck. We use a multi control block for our immuno's so I would like to find something common and normal/benign to use. Any help or suggestions would be appreciated. Thanks, David Hill Trumbull Labs Germantown, TN From Maxim_71 <@t> mail.ru Thu Nov 11 21:37:18 2010 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Nov 11 21:37:20 2010 Subject: [Histonet] Processing rodent tissue Message-ID: <298245371.20101112063718@mail.ru> John: Some labs uses processing metod with isopropanol and mineral oil with very good results. Here is our manual protocol for rodent tissues: 1. Isopropanol 70% 0.5 h 2. Isopropanol 80% 0.5 h 3. Isopropanol 95% 0.5 h 4. Isopropanol 99% 0.5 h 5. IM 5:1 50oC 1 h 6. IM 2:1 50oC 1 h 7. Mineral oil 1.5 h (or overnight) 8. Paraffin 0.5 h 9. Paraffin 0.5 h 10. Paraffin 20 min 11. Paraffin 20 min. Advantage of isopropanol consists in that ethanol have "tide" effect and do tissues hard, but isopropanol (IPA) not. Mineral oil = paraffin with low molecular weight and the infiltration is better and gentle. Mineral oil is absolutely not toxic for personnel. Transitions between IPA, MO and paraffin are very gentle. Please let me know about results. If you need any more details please contact to me. Best wishes, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From lpwenk <@t> sbcglobal.net Fri Nov 12 03:58:24 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Nov 12 03:58:32 2010 Subject: [Histonet] CK7/EMA control tissue In-Reply-To: References: Message-ID: <311243006A494A8AAE3B5A2CD7E649C3@HP2010> Some lung carcinoma could be positive for both. Since IHC is usually done on tissue with cancer, which often has low levels of antigens, I personally would rather use another cancer as a control, rather than a "normal/benign" tissue which would have high levels of the antigen. More likely to miss the low levels in patient tissue when using a too positive control. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "David Hill" Sent: Thursday, November 11, 2010 9:51 PM To: Subject: [Histonet] CK7/EMA control tissue > I'm looking to find some control tissue that will stain positive for ck7 > and ema. We are currently using breast carcinoma tissue but most of the > tumors we are seeing upon grossing are being submitted entirely for > pathologist review. I have been searching the literature for any normal > tissue that stains for ck7 and ema with no luck. We use a multi control > block for our immuno's so I would like to find something common and > normal/benign to use. Any help or suggestions would be appreciated. > > Thanks, > David Hill > Trumbull Labs > Germantown, TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Nov 12 04:45:00 2010 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Nov 12 04:45:09 2010 Subject: [Histonet] Histology Supervisor Job in Oklahoma In-Reply-To: <768330664.1289408366500.JavaMail.cfservice@SL4APP1> References: <768330664.1289408366500.JavaMail.cfservice@SL4APP1> Message-ID: Hi Alisha Thanks for the information. Starting in January, if you want to forward your postings to my work email at jweave01@cscc.edu I can also post them on our histology program web page and learning platform for histology students. This information can also make it to experienced histotechs working in our clinical affiliate laboratories. At some point, I plan to make a database with profiles of our graduates with their special experiences or abilities. This would be hopefully a two-way exchange, and voluntary of course, since some personal information would need to be shared for contacting individuals. I plan to include contact information for agencies as well, so that clients and candidates can get connected, without having to wait on me to pass along the message. I have a programmer working on the logistics right now.Let me know if you think this might be helpful to you. Thanks Joelle From: alisha@ka-recruiting.com To: histonet@lists.utsouthwestern.edu Date: Wed, 10 Nov 2010 11:59:26 -0500 Subject: [Histonet] Histology Supervisor Job in Oklahoma Hi Histonet Members, I am currently working on a Histology Supervisor position in OK. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Nov 12 08:26:55 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Nov 12 08:27:20 2010 Subject: [Histonet] Histo data needed Message-ID: A big thank you to everyone who responded to my post. All of the responses were helpful! - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 From laurie.colbert <@t> huntingtonhospital.com Fri Nov 12 08:42:02 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Nov 12 08:42:07 2010 Subject: [Histonet] Formalin In-Reply-To: <201011112244.oABMica0029530@imr-ma03.mx.aol.com> Message-ID: <57BE698966D5C54EAE8612E8941D768309E91B7B@EXCHANGE3.huntingtonhospital.com> Not us - in Pasadena CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, November 11, 2010 2:45 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Ok, so how many of you are allowed to dump formalin down the drain? Sent from my Verizon Wireless Phone From rmweber113 <@t> comcast.net Fri Nov 12 08:48:17 2010 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Fri Nov 12 08:48:19 2010 Subject: [Histonet] Needle Core Bxs In-Reply-To: <2130938049.11269.1289573208552.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Message-ID: <745265815.11387.1289573297892.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Good Morning,? Does anyone have a manual hand dip?method to process needle core biopsy tissue?? Also looking for a good microwave method as well for a Thermo Shandon Tissue Wave 2. Thanks,? Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 From MSHERWOOD <@t> PARTNERS.ORG Fri Nov 12 08:53:35 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Nov 12 08:53:48 2010 Subject: [Histonet] Formalin In-Reply-To: <2C3A3222-C26B-4AB8-ACCB-748F9660E270@imagesbyhopper.com> References: <201011112244.oABMica0029530@imr-ma03.mx.aol.com><929240319-1289522732-cardhu_decombobulator_blackberry.rim.net-89595305-@bda551.bisx.prod.on.blackberry> <2C3A3222-C26B-4AB8-ACCB-748F9660E270@imagesbyhopper.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB51E8@PHSXMB30.partners.org> Ditto in Massachusetts. I work at MGH and we are striclty regulated by the MWRA (Mass Water Resources Association) which monitors closely anything that goes down the drain. They are especially concerned with mercury and the ppm are very minute. Anything going down the drain can affect Boston Harbor. We have an outside company that collects our waste, including ethanol. We throw nothing down the drain. Peg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, November 11, 2010 9:37 PM To: jmacdonald@mtsac.edu Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin Not in Florida either. We use Aldex to neutralize the formalin & then dispose of it on the biohazard trash. The mfr says once neutralized you can put it in regular trash, but we don't. Michelle On Nov 11, 2010, at 7:45 PM, jmacdonald@mtsac.edu wrote: > Absolutely not here in southern California. > > > Sent via BlackBerry by AT&T > > -----Original Message----- > From: "godsgalnow@aol.com" > Sender: histonet-bounces@lists.utsouthwestern.edu > Date: Thu, 11 Nov 2010 17:44:52 > To: > Subject: [Histonet] Formalin > > Ok, so how many of you are allowed to dump formalin down the drain? > > Sent from my Verizon Wireless Phone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From W.E.J.Hoekert <@t> olvg.nl Fri Nov 12 09:45:56 2010 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Fri Nov 12 09:46:51 2010 Subject: [Histonet] Formalin References: <57BE698966D5C54EAE8612E8941D768309E91B7B@EXCHANGE3.huntingtonhospital.com> Message-ID: <1190CB05C44B13409483514729C2FC360C0B35@PAIT42.olvg.nl> Not here in The Netherlands. ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Laurie Colbert Verzonden: vr 12/11/2010 15:42 Aan: godsgalnow@aol.com; Histonet@lists.utsouthwestern.edu Onderwerp: RE: [Histonet] Formalin Not us - in Pasadena CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, November 11, 2010 2:45 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Ok, so how many of you are allowed to dump formalin down the drain? Sent from my Verizon Wireless Phone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From Valerie.Hannen <@t> parrishmed.com Fri Nov 12 10:06:00 2010 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Fri Nov 12 10:06:49 2010 Subject: [Histonet] Formalin References: <201011112244.oABMica0029530@imr-ma03.mx.aol.com> <929240319-1289522732-cardhu_decombobulator_blackberry.rim.net-89595305-@bda551.bisx.prod.on.blackberry> Message-ID: <5680DA93771F0C48954CC8D38425E72401AD03DC@ISMAIL.parrishmed.local> No way in Florida!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jmacdonald@mtsac.edu Sent: Thu 11/11/2010 7:45 PM To: godsgalnow@aol.com; histonet-bounces@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin Absolutely not here in southern California. Sent via BlackBerry by AT&T -----Original Message----- From: "godsgalnow@aol.com" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Thu, 11 Nov 2010 17:44:52 To: Subject: [Histonet] Formalin Ok, so how many of you are allowed to dump formalin down the drain? Sent from my Verizon Wireless Phone ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From JWeems <@t> sjha.org Fri Nov 12 10:07:25 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Nov 12 10:07:35 2010 Subject: [Histonet] Formalin In-Reply-To: <1190CB05C44B13409483514729C2FC360C0B35@PAIT42.olvg.nl> References: <57BE698966D5C54EAE8612E8941D768309E91B7B@EXCHANGE3.huntingtonhospital.com> <1190CB05C44B13409483514729C2FC360C0B35@PAIT42.olvg.nl> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044A071FDC@CHEXCMS10.one.ads.che.org> Not here in Atlanta until it it neutralized. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hoekert, W.E.J. Sent: Friday, November 12, 2010 10:46 To: Laurie Colbert; godsgalnow@aol.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin Not here in The Netherlands. ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Laurie Colbert Verzonden: vr 12/11/2010 15:42 Aan: godsgalnow@aol.com; Histonet@lists.utsouthwestern.edu Onderwerp: RE: [Histonet] Formalin Not us - in Pasadena CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, November 11, 2010 2:45 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Ok, so how many of you are allowed to dump formalin down the drain? Sent from my Verizon Wireless Phone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From gu.lang <@t> gmx.at Fri Nov 12 10:12:52 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Nov 12 10:13:01 2010 Subject: [Histonet] question to ventana benchmark XT and ultra users Message-ID: <04ADD883D3944850B4032A685A2AA509@dielangs.at> Hi! This week we've got the Ultra. In the first run it happend, that the "hammer" touched a Prep-Kit from the side while rotating and pulled the whole reagens-rack down. (That makes a very uncomfortable noise.) We saw, that the distance between the hammer and the Prep-Kits is very small, and it differs from Prep-Kit to Prep-Kit. Has anyone of the Ventana Users similar experiences? Bye Gudrun Lang Histolab, Linz, Austria From STACEY.LANGENBERG <@t> UCDENVER.EDU Fri Nov 12 10:22:12 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Fri Nov 12 10:22:49 2010 Subject: [Histonet] question to ventana benchmark XT and ultra users In-Reply-To: <04ADD883D3944850B4032A685A2AA509@dielangs.at> References: <04ADD883D3944850B4032A685A2AA509@dielangs.at> Message-ID: <654559346.517672.1289578939750.JavaMail.rim@bda341.bisx.prod.on.blackberry> We have had the ultras for over a year now and that is a new one. Was the rack of antibodies not seated properly? Just a thought. Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: Gudrun Lang Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Fri, 12 Nov 2010 09:12:52 To: histonet@lists.utsouthwestern.edu Reply-To: "gu.lang@gmx.at" Subject: [Histonet] question to ventana benchmark XT and ultra users Hi! This week we've got the Ultra. In the first run it happend, that the "hammer" touched a Prep-Kit from the side while rotating and pulled the whole reagens-rack down. (That makes a very uncomfortable noise.) We saw, that the distance between the hammer and the Prep-Kits is very small, and it differs from Prep-Kit to Prep-Kit. Has anyone of the Ventana Users similar experiences? Bye Gudrun Lang Histolab, Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Fri Nov 12 10:41:25 2010 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Fri Nov 12 10:41:34 2010 Subject: [Histonet] question to ventana benchmark XT and ultra users In-Reply-To: <654559346.517672.1289578939750.JavaMail.rim@bda341.bisx.prod.on.blackberry> References: <04ADD883D3944850B4032A685A2AA509@dielangs.at> <654559346.517672.1289578939750.JavaMail.rim@bda341.bisx.prod.on.blackberry> Message-ID: Make sure that the lids on your prep-kit dispensers are pushed all the way down. Since the space between the hammer and the tops of the dispensers is so small, if one lid is not pushed down all the way the hammer will grab it. Seen it happen many times myself! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Langenberg, Stacey Sent: Friday, November 12, 2010 11:22 AM To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] question to ventana benchmark XT and ultra users We have had the ultras for over a year now and that is a new one. Was the rack of antibodies not seated properly? Just a thought. Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: Gudrun Lang Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Fri, 12 Nov 2010 09:12:52 To: histonet@lists.utsouthwestern.edu Reply-To: "gu.lang@gmx.at" Subject: [Histonet] question to ventana benchmark XT and ultra users Hi! This week we've got the Ultra. In the first run it happend, that the "hammer" touched a Prep-Kit from the side while rotating and pulled the whole reagens-rack down. (That makes a very uncomfortable noise.) We saw, that the distance between the hammer and the Prep-Kits is very small, and it differs from Prep-Kit to Prep-Kit. Has anyone of the Ventana Users similar experiences? Bye Gudrun Lang Histolab, Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Nov 12 10:52:25 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Nov 12 10:52:37 2010 Subject: AW: [Histonet] question to ventana benchmark XT and ultra users In-Reply-To: References: <04ADD883D3944850B4032A685A2AA509@dielangs.at> <654559346.517672.1289578939750.JavaMail.rim@bda341.bisx.prod.on.blackberry> Message-ID: Thanks Clare, Since we know the problem, we always press the lid very strongly down. And with the XT we were able to react sometimes, when we heard the clacking sound, and cut off the lid while the instrument was running. The Ultra is closed. I ask because the people of Ventana never heard of such a problem. And it seems, that we are the first on the planet to deal with it. ;) Gudrun -----Urspr?ngliche Nachricht----- Von: Clare Thornton [mailto:CThornton@dahlchase.com] Gesendet: Freitag, 12. November 2010 17:41 An: 'Langenberg, Stacey'; gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] question to ventana benchmark XT and ultra users Make sure that the lids on your prep-kit dispensers are pushed all the way down. Since the space between the hammer and the tops of the dispensers is so small, if one lid is not pushed down all the way the hammer will grab it. Seen it happen many times myself! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Langenberg, Stacey Sent: Friday, November 12, 2010 11:22 AM To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] question to ventana benchmark XT and ultra users We have had the ultras for over a year now and that is a new one. Was the rack of antibodies not seated properly? Just a thought. Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: Gudrun Lang Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Fri, 12 Nov 2010 09:12:52 To: histonet@lists.utsouthwestern.edu Reply-To: "gu.lang@gmx.at" Subject: [Histonet] question to ventana benchmark XT and ultra users Hi! This week we've got the Ultra. In the first run it happend, that the "hammer" touched a Prep-Kit from the side while rotating and pulled the whole reagens-rack down. (That makes a very uncomfortable noise.) We saw, that the distance between the hammer and the Prep-Kits is very small, and it differs from Prep-Kit to Prep-Kit. Has anyone of the Ventana Users similar experiences? Bye Gudrun Lang Histolab, Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Fri Nov 12 11:07:05 2010 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Fri Nov 12 11:07:11 2010 Subject: [Histonet] Waste down the drain Message-ID: <379A927A452F3D43A3C8705F4E67905F165C3A3059@EX05.net.ucsf.edu> In reading all the comments about waste down the drain, proprietary ingredients, etc., keep in mind that disposal regulations are different everywhere and NO vendor will tell you anything other than to follow those regs and/or play it safe and tell you to haul it away. At a previous employer in San Francisco I used Ventana immuno stainers and we hauled the waste away. It was expensive to haul away, and was difficult to get guidance on disposal because of the proprietary ingedients in the waste mix. So our lab director sent a sample of the waste from the the stainers to an environmental services lab for testing. The results of the LD50 and other analyses apparently indicated that the actual toxicity was quite low. He then sent the testing report to the city/county for instructions on how to dispose of the waste. The city/county responded in writing (good for records!) that it was permissible to dump it down the drain. I wasn't thrilled with it, but our lab director was. We kept that report and the response from the city/county on file to show to inspectors. It's up to you to figure out the appropriate way to handle your waste in your area, not your vendor. There's no way they would know all the different regulations for every state, city or town. They are all different and quite confusing. The cost of the lab test might be a worthwhile investment because you would have specifics to show your environmental safety people or local regulatory agency, which would make it easier for them to give you guidance. Erin Martin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 From af46 <@t> buffalo.edu Fri Nov 12 11:19:11 2010 From: af46 <@t> buffalo.edu (Annette Featherstone) Date: Fri Nov 12 11:19:18 2010 Subject: [Histonet] RAGE and AGE Message-ID: Is anyone using RAGE or AGE antibody successfully? If so would you be willing to share vendor and data? I am also looking for a Tunel Assay kit, what would any of you recommend? Thanks, Annette Featherstone From NHeath <@t> Lifespan.org Fri Nov 12 12:24:34 2010 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Fri Nov 12 12:24:59 2010 Subject: [Histonet] RAGE and AGE In-Reply-To: Message-ID: <130E8991F210424096EFC6F42EA33B2406E3FC73@LSCOEXCH1.lsmaster.lifespan.org> RAGE and AGE - yes rage gets worse with age...it's called menopause....i just had too it's FRIDAY!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone Sent: Friday, November 12, 2010 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RAGE and AGE Is anyone using RAGE or AGE antibody successfully? If so would you be willing to share vendor and data? I am also looking for a Tunel Assay kit, what would any of you recommend? Thanks, Annette Featherstone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kenneth.a.troutman <@t> Vanderbilt.Edu Fri Nov 12 12:34:17 2010 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Nov 12 12:37:16 2010 Subject: [Histonet] question to ventana benchmark XT and ultra Message-ID: <7B310892042DA74CB3590053F424CFE6138205BB51@ITS-HCWNEM06.ds.Vanderbilt.edu> You might not have seated the top of the prep kit container all the way down. They should not be different heights. I would take each of them and press down on the top to make sure the lid is on properly. If you set them side by side and they are still different heights, I would contact your Ventana rep immediately and show them this. Otherwise, I am inclined to agree with Clare that the reagent trays were not in the proper position. You should have about a half inch of clearance between the hammer and the top of the prep kit container. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 1 Date: Fri, 12 Nov 2010 11:41:25 -0500 From: Clare Thornton Subject: RE: [Histonet] question to ventana benchmark XT and ultra users To: "'Langenberg, Stacey'" , "gu.lang@gmx.at" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Make sure that the lids on your prep-kit dispensers are pushed all the way down. Since the space between the hammer and the tops of the dispensers is so small, if one lid is not pushed down all the way the hammer will grab it. Seen it happen many times myself! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Langenberg, Stacey Sent: Friday, November 12, 2010 11:22 AM To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] question to ventana benchmark XT and ultra users We have had the ultras for over a year now and that is a new one. Was the rack of antibodies not seated properly? Just a thought. Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: Gudrun Lang Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Fri, 12 Nov 2010 09:12:52 To: histonet@lists.utsouthwestern.edu Reply-To: "gu.lang@gmx.at" Subject: [Histonet] question to ventana benchmark XT and ultra users Hi! This week we've got the Ultra. In the first run it happend, that the "hammer" touched a Prep-Kit from the side while rotating and pulled the whole reagens-rack down. (That makes a very uncomfortable noise.) We saw, that the distance between the hammer and the Prep-Kits is very small, and it differs from Prep-Kit to Prep-Kit. Has anyone of the Ventana Users similar experiences? Bye Gudrun Lang From Maria.Mejia <@t> ucsf.edu Fri Nov 12 13:06:08 2010 From: Maria.Mejia <@t> ucsf.edu (Mejia, Maria) Date: Fri Nov 12 13:06:16 2010 Subject: [Histonet] RE: Vibratome sectioning of mouse spinal cord In-Reply-To: References: Message-ID: Hello Susan, Before I can help, I need more information from you. Are your samples fixed or unfixed? In any case, make sure that the vibratome is in working order & that all the movable parts are tight before you section on the vibratome. In addition, during cutting the holding tray should have cold 0.1M PB buffer & this tray is surrounded by wet ice. The temperature should be cold during sectioning. I don't know what type of vibratome your using, but check the angle of the blade - this may need to be changed slightly - check what the manual says because it should be possible to use the knife at different angles. Also check the knife blade, you most likely have already used a new blade. Finally, but important during the cutting as the vibratome begins to section & you actually see the section starting to come off - use your brush to brush away the tissue section from the knife edge during cutting - this should keep the tissue flat. Also, maybe try cutting at 50 microns. Hope this helps you! Maria Mejia Senior Histology Supervisor Department of Neurosurgery UCSF San Francisco, CA 94103 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael, Susan [michaels@janelia.hhmi.org] Sent: Thursday, November 11, 2010 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Vibratome sectioning of mouse spinal cord Can anyone give me some tips on sectioning mouse spinal cord on the vibratome? Specifically, once the dura is successfully removed, I have been sectioning 40 um horizontal sections, and some cords have been curling up on themselves, both rostral-caudal and medial-lateral, and are impossible to uncurl without destroying the tissue. I?ve tried every angle of approach, speed, amplitude, the tissue has been in sucrose, embedded in agarose, and chilled. Thanks, Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dbeil1 <@t> hotmail.com Fri Nov 12 14:05:43 2010 From: dbeil1 <@t> hotmail.com (Damaris Beil) Date: Fri Nov 12 14:05:47 2010 Subject: [Histonet] Minotome cryostats Message-ID: Hello, I would to know if anyone knows how to get two Minotome cryostats apraised. They were manufactured by Damon/IEC Division and remanufactured by Belair Instruments. Thank You, From macveigh <@t> usc.edu Fri Nov 12 14:52:09 2010 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Fri Nov 12 14:52:15 2010 Subject: [Histonet] Formalin Message-ID: <002901cb82ab$79961ad0$6cc25070$@usc.edu> Absolutely not!!! In California. Safety office picks it up and dispose of it. Michelle Aloni From gu.lang <@t> gmx.at Fri Nov 12 15:22:13 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Nov 12 15:22:20 2010 Subject: AW: [Histonet] question to ventana benchmark XT and ultra In-Reply-To: <7B310892042DA74CB3590053F424CFE6138205BB51@ITS-HCWNEM06.ds.Vanderbilt.edu> References: <7B310892042DA74CB3590053F424CFE6138205BB51@ITS-HCWNEM06.ds.Vanderbilt.edu> Message-ID: <3A3AFFA711E042469266970D6ADBAD29@dielangs.at> Hi Ashley, the clearance is between 1-3 mm and I think that's too small. In a few days Ventana will lift the arm with the hammer for additional 3 mm. That should help for a reliable working of the instrument. Half an inch is about 12 mm. I think, not even with the detection-kit-preps this clearance is reached on our instrument. What was astonishing for me is, that they never heard about such problems. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Troutman, Kenneth A Gesendet: Freitag, 12. November 2010 19:34 An: 'Histonet@lists.utsouthwestern.edu' Betreff: RE: [Histonet] question to ventana benchmark XT and ultra You might not have seated the top of the prep kit container all the way down. They should not be different heights. I would take each of them and press down on the top to make sure the lid is on properly. If you set them side by side and they are still different heights, I would contact your Ventana rep immediately and show them this. Otherwise, I am inclined to agree with Clare that the reagent trays were not in the proper position. You should have about a half inch of clearance between the hammer and the top of the prep kit container. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 1 Date: Fri, 12 Nov 2010 11:41:25 -0500 From: Clare Thornton Subject: RE: [Histonet] question to ventana benchmark XT and ultra users To: "'Langenberg, Stacey'" , "gu.lang@gmx.at" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Make sure that the lids on your prep-kit dispensers are pushed all the way down. Since the space between the hammer and the tops of the dispensers is so small, if one lid is not pushed down all the way the hammer will grab it. Seen it happen many times myself! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Langenberg, Stacey Sent: Friday, November 12, 2010 11:22 AM To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] question to ventana benchmark XT and ultra users We have had the ultras for over a year now and that is a new one. Was the rack of antibodies not seated properly? Just a thought. Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: Gudrun Lang Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Fri, 12 Nov 2010 09:12:52 To: histonet@lists.utsouthwestern.edu Reply-To: "gu.lang@gmx.at" Subject: [Histonet] question to ventana benchmark XT and ultra users Hi! This week we've got the Ultra. In the first run it happend, that the "hammer" touched a Prep-Kit from the side while rotating and pulled the whole reagens-rack down. (That makes a very uncomfortable noise.) We saw, that the distance between the hammer and the Prep-Kits is very small, and it differs from Prep-Kit to Prep-Kit. Has anyone of the Ventana Users similar experiences? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stevenhacker <@t> verizon.net Fri Nov 12 15:30:29 2010 From: stevenhacker <@t> verizon.net (Steven Hacker) Date: Fri Nov 12 15:30:39 2010 Subject: [Histonet] Waste down the drain Message-ID: <13434166.637878.1289597429844.JavaMail.root@vms069.mailsrvcs.net> The EPA will not agree to biohazardous waste down the drain even if a biohazardou surprise inspections for informed by the EPA that if the the drain it was big time fines. One o inspection at the time of evaluation and were told that it was ok-in front of the EPA inspector! Facilities been told that if you pour bleach into the waste it'll neutraliz the waste. Once again, according to the EPA '...it may reduce the biohaza waste. You will a metal' and also cannot be Regards, Steven Hacker Biocare Medical. Nov 12, 2010 12:08:48 PM, Erin.Martin@ucsf In reading al proprietary ingredients, etc., k regulations are different everywhere and NO vendo anything other than to follow those regs and/or play it saf tell you to haul it away. At a previous employer in San Francisco I u expensive disposal because of the So our lab director sent a sample stainers to an environmental services lab for tes results of the LD50 and other analyses apparently indicated that the actual toxicity was quite low. He then sent the testing report to the c waste. The city/county that it was permissible to dump i thrilled with it, but our lab director was. We k and the response from the city/county on file to show to in spectors. It's up to you to figure out the appropriate way to handle waste in your area, not your vendor. There's no way they would know a are all di might be a worthwhile to show your environmental saf agency, which would make it easier for them Erin Martin Erin Martin, Histology UCSF Department of Dermatopathology 415-353-7248 __ Histonet mailing list H http://lists.utsouthwestern.edu/mailman From sfeher <@t> CMC-NH.ORG Fri Nov 12 15:31:05 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Nov 12 15:31:12 2010 Subject: [Histonet] Waste down the drain In-Reply-To: <379A927A452F3D43A3C8705F4E67905F165C3A3059@EX05.net.ucsf.edu> References: <379A927A452F3D43A3C8705F4E67905F165C3A3059@EX05.net.ucsf.edu> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC243867CD@exchange.cmc-nh.org> Great advice Erin! We did the same thing with the waste from our Dako Artisan to document the percentages of trace metals. Again, they were too low to require special handling over and above what we normally do with our hazardous waste. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Friday, November 12, 2010 12:07 PM To: histonet Subject: [Histonet] Waste down the drain In reading all the comments about waste down the drain, proprietary ingredients, etc., keep in mind that disposal regulations are different everywhere and NO vendor will tell you anything other than to follow those regs and/or play it safe and tell you to haul it away. At a previous employer in San Francisco I used Ventana immuno stainers and we hauled the waste away. It was expensive to haul away, and was difficult to get guidance on disposal because of the proprietary ingedients in the waste mix. So our lab director sent a sample of the waste from the the stainers to an environmental services lab for testing. The results of the LD50 and other analyses apparently indicated that the actual toxicity was quite low. He then sent the testing report to the city/county for instructions on how to dispose of the waste. The city/county responded in writing (good for records!) that it was permissible to dump it down the drain. I wasn't thrilled with it, but our lab director was. We kept that report and the response from the city/county on file to show to inspectors. It's up to you to figure out the appropriate way to handle your waste in your area, not your vendor. There's no way they would know all the different regulations for every state, city or town. They are all different and quite confusing. The cost of the lab test might be a worthwhile investment because you would have specifics to show your environmental safety people or local regulatory agency, which would make it easier for them to give you guidance. Erin Martin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Fri Nov 12 17:01:30 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Nov 12 17:01:35 2010 Subject: [Histonet] Rapid H&E Message-ID: <57BE698966D5C54EAE8612E8941D768309E91C94@EXCHANGE3.huntingtonhospital.com> Our pathologists want a rapid H&E stain to set up in CT to determine the adequacy of lung FNA's. They do not want a diff quik stain. Any suggestions on something fast and easy that doesn't require a lot rinsing? Laurie Colbert From dbeil1 <@t> hotmail.com Fri Nov 12 17:42:50 2010 From: dbeil1 <@t> hotmail.com (Damaris Beil) Date: Fri Nov 12 17:42:54 2010 Subject: [Histonet] Routine histology equipment Message-ID: Hello, I was wondering if anyone who is using the ThermoFisher histology equipment would mind sharing any thoughts regarding their experience with this equipment. I'm looking at the Excelsior processor, gem ES H&E stainer, clearvue coverslipper, print mate and slide mate (cassette and slide labelers). Your help will be really appreciated. Thanks, From dbeil1 <@t> hotmail.com Fri Nov 12 17:53:38 2010 From: dbeil1 <@t> hotmail.com (Damaris Beil) Date: Fri Nov 12 17:53:43 2010 Subject: [Histonet] Refurbished histology Leica equipment Message-ID: Hello, I would like to know if anyone has had any experience using a refurbished Leica XL auto stainer and coverslip CV5000. Any thoughts you would not mind sharing? Thanks in advance, From Bill.Tench <@t> pph.org Fri Nov 12 18:00:14 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Fri Nov 12 18:00:25 2010 Subject: [Histonet] rapid h and e Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A580F@MAIL1.pph.local> We use the same materials and timing we use for a frozen section, which as a cytopathologist works just fine for me. Approx 1 min Hematox, several dips wash, dips in blueing UNTIL BLUE (people tend to shorten this critical step), no more than 5 dips in eos, 100 ETOH X 2, xylene dipped until runs smooth, coverslip. Takes about 1 min 45 sec. If there was a lot of blood, one can start with quick fix and dips in acid alcohol, or the histo people can destain and restain the case at a later date. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From brandihiggins <@t> gmail.com Fri Nov 12 22:25:37 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Fri Nov 12 22:25:46 2010 Subject: [Histonet] rapid h and e In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A580F@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A580F@MAIL1.pph.local> Message-ID: We used to do the similar to what Bill Tench described below. However, destaining and restaining with Pap caused loss of some of the cells and the pathologists prefer the pap for these smears so we devised a Stat Pap stain: from alcohol, 50% alcohol, water, Gil's Hematoxylin for 1 min, water, bluing, water, 50% alcohol, alcohol OG6 10 dips alcohol EA50 10 dips alcohol, clearing agent, coverslip. The pathologists have been really happy with this, we've been using it for about 7 months no with no complaints other than once they said stain was too dark, but smear was too thick in some areas. Hope this helps. Brandi Higgins On Fri, Nov 12, 2010 at 7:00 PM, Tench, Bill wrote: > We use the same materials and timing we use for a frozen section, which > as a cytopathologist works just fine for me. Approx 1 min Hematox, > several dips wash, dips in blueing UNTIL BLUE (people tend to shorten > this critical step), no more than 5 dips in eos, 100 ETOH X 2, xylene > dipped until runs smooth, coverslip. Takes about 1 min 45 sec. If > there was a lot of blood, one can start with quick fix and dips in acid > alcohol, or the histo people can destain and restain the case at a later > date. > > Bill Tench > Associate Dir. Laboratory Services > Chief, Cytology Services > Palomar Medical Center > 555 E. Valley Parkway > Escondido, California 92025 > Bill.Tench@pph.org > Voice: 760- 739-3037 > Fax: 760-739-2604 > > > [None] made the following annotations > --------------------------------------------------------------------- > Confidential E-Mail: This e-mail is intended only for the person or entity > to which it is addressed, and may contain information that is privileged, > confidential, or otherwise protected from disclosure. Dissemination, > distribution, or copying of this e-mail or the information herein by anyone > other than the intended recipient is prohibited. If you have received this > e-mail in error, please notify the sender by reply e-mail, and destroy the > original message and all copies. > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bakevictoria <@t> gmail.com Sat Nov 13 08:32:48 2010 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Sat Nov 13 08:32:57 2010 Subject: [Histonet] RAGE and AGE In-Reply-To: <130E8991F210424096EFC6F42EA33B2406E3FC73@LSCOEXCH1.lsmaster.lifespan.org> References: <130E8991F210424096EFC6F42EA33B2406E3FC73@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: Now they have that in a musical which is absolutely hysterical - Menopause - The Musical. When it comes to your area it is a must see for any going through this wonderful stage in life. Vikki On Fri, Nov 12, 2010 at 1:24 PM, Heath, Nancy L. wrote: > RAGE and AGE - yes rage gets worse with age...it's called menopause....i > just had too it's FRIDAY!! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette > Featherstone > Sent: Friday, November 12, 2010 12:19 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RAGE and AGE > > Is anyone using RAGE or AGE antibody successfully? If so would you be > willing to share vendor and data? > > I am also looking for a Tunel Assay kit, what would any of you > recommend? > > Thanks, > > Annette Featherstone > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dbeil1 <@t> hotmail.com Sat Nov 13 08:41:49 2010 From: dbeil1 <@t> hotmail.com (Damaris Beil) Date: Sat Nov 13 08:41:54 2010 Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) Message-ID: Hello, I'm interested in finding out if anyone is using ThermoFisher's new PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) and how they are working out for you. Thanks in advance for your help, Damaris From Bill.Tench <@t> pph.org Sat Nov 13 11:09:56 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Sat Nov 13 11:10:09 2010 Subject: [Histonet] rapid h and e Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5811@MAIL1.pph.local> We have never noticed any significant cell loss if "dehemoglobinzing" is done on bloody smears either before or after regular staining (as noted, this is used ONLY when the smear is very bloody. If it is very bloody there can be very significant obscuration. This is especially true of liver FNA's). As for regular staining, we have not found that the addition of OG offers anything special (the rare case of keratinized cells still stands out with the regular h and e stain). Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From jnocito <@t> satx.rr.com Sat Nov 13 12:35:17 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Nov 13 12:35:42 2010 Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) In-Reply-To: References: Message-ID: we've had them for over two years and we still don't have the system up and running (for various reasons, some internal issues, some external issues). We had to stop automatically sending the slide queue from the cassette queue because of a lot of confusion with extra slides. A lot of slides were being wasted. We had to send the Checkmates back because the screens became dull, couldn't read the screen. They were fixed, I still don't know what the problem was. I even changed the battery on each machine. One time I called Thermofisher (or whatever they're called this week) because our cassette catcher wasn't working. I was told that they didn't have a manual for the Printmate and that it would cost $2000 to send out a rep to JUST look at it. I called my rep and asked what kind of crap that was. She told be to call the help desk, where I did get help and was able to fix it over the phone (thank you Cherryll). Personally, I would not have purchased it. The Printmate has some quirks in it, even with the software up date. Sometimes, the machine looses it position. You have to close down the software, shut the machine off, turn the machine back on, wait for it to boot up, then open up the software. You have to use their cassettes because of the angle of the front of the cassette. We have tried other brands and only half the numbers were printing on the cassette. As some of you know, I really hate equipment that you have to USE their brand or it won't work. (remember my Ventana rants). Wow, all that anger, frustration, just spilling out. not that I have anger issues. I've been to anger management classes 5 times. Joe ----- Original Message ----- From: "Damaris Beil" To: "histonet" Sent: Saturday, November 13, 2010 8:41 AM Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) Hello, I'm interested in finding out if anyone is using ThermoFisher's new PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) and how they are working out for you. Thanks in advance for your help, Damaris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From STACEY.LANGENBERG <@t> UCDENVER.EDU Sat Nov 13 22:52:15 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Sat Nov 13 22:52:29 2010 Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) andSlide Mate (slide labeler with scanner) In-Reply-To: References: Message-ID: <1441692577.553925.1289710339371.JavaMail.rim@bda341.bisx.prod.on.blackberry> We love our Leica IPS and IPC slide and cassette printers! I would highly recommend them to you Joe! Lose the printmates. Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: Joe Nocito Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Sat, 13 Nov 2010 11:35:17 To: Damaris Beil; histonet Subject: Re: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) we've had them for over two years and we still don't have the system up and running (for various reasons, some internal issues, some external issues). We had to stop automatically sending the slide queue from the cassette queue because of a lot of confusion with extra slides. A lot of slides were being wasted. We had to send the Checkmates back because the screens became dull, couldn't read the screen. They were fixed, I still don't know what the problem was. I even changed the battery on each machine. One time I called Thermofisher (or whatever they're called this week) because our cassette catcher wasn't working. I was told that they didn't have a manual for the Printmate and that it would cost $2000 to send out a rep to JUST look at it. I called my rep and asked what kind of crap that was. She told be to call the help desk, where I did get help and was able to fix it over the phone (thank you Cherryll). Personally, I would not have purchased it. The Printmate has some quirks in it, even with the software up date. Sometimes, the machine looses it position. You have to close down the software, shut the machine off, turn the machine back on, wait for it to boot up, then open up the software. You have to use their cassettes because of the angle of the front of the cassette. We have tried other brands and only half the numbers were printing on the cassette. As some of you know, I really hate equipment that you have to USE their brand or it won't work. (remember my Ventana rants). Wow, all that anger, frustration, just spilling out. not that I have anger issues. I've been to anger management classes 5 times. Joe ----- Original Message ----- From: "Damaris Beil" To: "histonet" Sent: Saturday, November 13, 2010 8:41 AM Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) Hello, I'm interested in finding out if anyone is using ThermoFisher's new PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) and how they are working out for you. Thanks in advance for your help, Damaris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sandoval.1965 <@t> hotmail.com Sun Nov 14 05:27:05 2010 From: sandoval.1965 <@t> hotmail.com (Anthony Sandoval) Date: Sun Nov 14 05:27:13 2010 Subject: [Histonet] Hey let's get this money together Message-ID: Hi histo, we haven't talked in a while... I just wanted to share something with you. My biggest day making money online was $430 dollars and I just started last week! Start by reading how to do it here, then get them to send the kit to your house. It's free! http://bit.ly/9am423 From axp969 <@t> psu.edu Sun Nov 14 10:38:57 2010 From: axp969 <@t> psu.edu (ANJUM PARKAR) Date: Sun Nov 14 10:39:02 2010 Subject: [Histonet] Frozen brain slices Message-ID: <1289752737l.1429610l.0l@psu.edu> Hello all, I have been having some histology issues in terms of rat brain slicing the past few months and hence looking for suggestions to fix them, having tried most of what I know from my past training and running of ideas real fast now. The following is the protocol I have used thus far:-1. Animal perfusion-a)Use PBS first, b) Use 4%PFA next and c) Harvest brain right after PFA. (Transcardial perfusion)2. Freeze brain by embedding in OCT compound using dry ice and then storing at -80 until ready to slice. Alternatively some times the -80 is used straight up with out dry ice freezing (still embedded in OCT).3. >From standard protocol I do not do two things-a) No post fixation once brain is harvested and b) No sucrose. Reason being, I was taught that these two steps might result in large holes in tissue.4.For slicing, we have the older cryostat version (Real old version of CM3050S from Vibrotome-30 micron slices) which uses a fixed knife for slicing and has a chamber temp control and one for the specimen. The cryostat has not been serviced for a few years now.5. In attempt 1-I take the brain out of the -80 once the chamber temperature comes to -20 and mount the frozen mold on the chuck and attempt at slicing. Doing this I managed to get a few slices (that too with cuts and ripples) and after which the tissue just kept cracking and falling into pieces and hence could get no more slices.6. In attempt 2-I thought the brain may be too cold and too hard (perfusion and-80 freezing), so I defrosted the brain for 20 mins at room temperature before sticking into -20 cryostat. Between slices if I felt the tissue was too cold, I put my thumb on the specimen to warm it up. Still no luck.7. In attempt 3-I defrosted the brain completely to room temperature, took the brain out of the cryogel embedding (thinking that the mold was way too hard to cut through and hence cracking the tissue too) and attached it to chuck directly and tried slicing. Still no luck at all this time around. >From what I have been reading online and from what I know, I primarily feel these are issues related with temperature of the brain and that it is too cold during cutting. I have tried different ways to bring the temperature down and I still have no success. I have eight other brains frozen using the protocol above and I really need to get slices out of them. Do you have any suggestions for me? Also do you think I should make any changes to the existing freezing protocol for the future brain harvesting? APPenn State University,PA From akemiat3377 <@t> yahoo.com Sun Nov 14 10:58:39 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Sun Nov 14 10:58:52 2010 Subject: SPAM? [Histonet] Hey let's get this money together In-Reply-To: References: Message-ID: <655689CA-59F4-4488-AAAB-C55F9000A7EC@yahoo.com> Hey all, Wouldn't this email be considered a SPAM? I realize the histonet is a forum, but not one for sales!!!!! Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Nov 14, 2010, at 3:27 AM, Anthony Sandoval wrote: > Hi histo, we haven't talked in a while... I just wanted to share > something with you. My biggest day making money online was $430 > dollars and I just started last week! Start by reading how to do it > here, then get them to send the kit to your house. It's free! > http://bit.ly/9am423 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jacquitta <@t> earthlink.net Sun Nov 14 12:19:23 2010 From: jacquitta <@t> earthlink.net (Jacquitta Taylor) Date: Sun Nov 14 12:19:24 2010 Subject: [Histonet] (no subject) Message-ID: <915B93BF-FFAF-4B46-8BD1-AB47BCCB3692@earthlink.net> Sent from my iPod From axp969 <@t> psu.edu Sun Nov 14 20:53:48 2010 From: axp969 <@t> psu.edu (ANJUM PARKAR) Date: Sun Nov 14 20:53:59 2010 Subject: [Histonet] Frozen brain slices In-Reply-To: 50809B73-F3A5-41E8-A42E-23ED6268A2E5@pbrc.edu References: <1289752737l.1429610l.0l@psu.edu><50809B73-F3A5-41E8-A42E-23ED6268A2E5@pbrc .edu> Message-ID: <1289789628l.1421536l.0l@psu.edu> Thanks Tina. I will keep the sucrose suggestion in mind for future harvests. For now I need to figure out how to slice the already fixed frozen tissue. Will try to equilibrate in the cryostat itself as against room temperature and see how that goes. The knife angle is 13 degrees and recently sharpened and thickness of slices 30 microns which I believe is standard for sectioning. I have sectioned previously a lot so doing hands on is something I am familiar with. But I did realize every instrument is different and while doing a procedure it always needs to be optimized until it can become routine. Will let you know how things go. Anjum On Sun, Nov 14, 2010 02:21 PM, "Montina Van Meter" wrote: > Anhum, >1. You MUST cryoprotect the fixed tissue in 15-30% sucrose (until it >sinks) prior to cutting frozen sections. >2. Allow the -80C embedded tissue block to equilabrate to -20C in the >cryostat for 15-20min. >3. Check the knife angle. Thickness? >4. Change knife or sharpen and make sure all screws are tight. >5. Freez/thawing of block is not a good idea (especially since it's >not cryoprotected). You are introducing ice crystal artifact. >6. Check around your department (or Histology Core) to see if someone > >can instruct you on using the cryostat. It's always better to actually >watch someone in addition to receiving written instructions. > >Good luck, >Tina > > >Sent from my iPhone > >On Nov 14, 2010, at 10:47 AM, "ANJUM PARKAR" >wrote: > >> Hello all, >> I have been having some histology issues in terms of rat brain >> slicing the past >> few months and hence looking for suggestions to fix them, having >> tried most of >> what I know from my past training and running of ideas real fast now. >> The following is the protocol I have used thus far:-1. Animal >> perfusion-a)Use >> PBS first, b) Use 4%PFA next and c) Harvest brain right after >PFA. >> (Transcardial perfusion)2. Freeze brain by embedding in OCT >compound >> using dry >> ice and then storing at -80 until ready to slice. Alternatively some >> times the >> -80 is used straight up with out dry ice freezing (still embedded in >> OCT).3. >> From standard protocol I do not do two things-a) No post fixation >> once brain is >> harvested and b) No sucrose. Reason being, I was taught that these >> two steps >> might result in large holes in tissue.4.For slicing, we have the >> older cryostat >> version (Real old version of CM3050S from Vibrotome-30 micron >> slices) which >> uses a fixed knife for slicing and has a chamber temp control and >> one for the >> specimen. The cryostat has not been serviced for a few years now.5. >> In attempt >> 1-I take the brain out of the -80 once the chamber temperature comes >> to -20 and >> mount the frozen mold on the chuck and attempt at slicing. Doing >> this I managed >> to get a few slices (that too with cuts and ripples) and after >which >> the tissue >> just kept cracking and falling into pieces and hence could get no more >> slices.6. In attempt 2-I thought the brain may be too cold and too >> hard >> (perfusion and-80 freezing), so I defrosted the brain for 20 >mins at >> room >> temperature before sticking into -20 cryostat. Between slices if I >> felt the >> tissue was too cold, I put my thumb on the specimen to warm it up. >> Still no >> luck.7. In attempt 3-I defrosted the brain completely to room >> temperature, took >> the brain out of the cryogel embedding (thinking that the mold was >> way too hard >> to cut through and hence cracking the tissue too) and attached it to >> chuck >> directly and tried slicing. Still no luck at all this time around. >> From what I have been reading online and from what I know, I >> primarily feel >> these are issues related with temperature of the brain and that it >> is too cold >> during cutting. I have tried different ways to bring the temperature >> down and I >> still have no success. I have eight other brains frozen using the >> protocol >> above and I really need to get slices out of them. Do you have any >> suggestions >> for me? Also do you think I should make any changes to the existing >> freezing >> protocol for the future brain harvesting? >> APPenn State University,PA >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From mbrooks <@t> incytepathology.com Mon Nov 15 08:28:20 2010 From: mbrooks <@t> incytepathology.com (Matt Brooks) Date: Mon Nov 15 08:28:23 2010 Subject: [Histonet] IHC antibody protocols Message-ID: <706224670091FE47997AEF88EFADE7CA0194EA75@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello All, I need help with information on a few Ventana BenchMark Ultra or XT protocols. We are having trouble getting HCL (DBA.44) to work. We are also in the process of working up FOXP-1, TAI-1, and GCET-1. Your help is appreciated. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 From meghan.tucker <@t> yahoo.com Mon Nov 15 08:30:49 2010 From: meghan.tucker <@t> yahoo.com (Meghan Tucker) Date: Mon Nov 15 08:30:54 2010 Subject: [Histonet] Cardboard slide flat storage Message-ID: <931675.17966.qm@web113214.mail.gq1.yahoo.com> Good morning, ? Are there any suggestions on a?place to order a storage shelf for?cardboard slide flats, which may have 6-8 'cubbies' that can be used to organize the flats? ? Thanks! ? Meghan ? Meghan.Tucker@yahoo.com From axp969 <@t> psu.edu Mon Nov 15 09:24:23 2010 From: axp969 <@t> psu.edu (ANJUM PARKAR) Date: Mon Nov 15 09:24:45 2010 Subject: [Histonet] Frozen brain slices In-Reply-To: 53DBCC77-3C32-4D03-B9E6-14CF56F7458D@pbrc.edu References: <1289752737l.1429610l.0l@psu.edu><50809B73-F3A5-41E8-A42E-23ED6268A2E5@pbrc .edu> <1289789628l.1421536l.0l@psu.edu><53DBCC77-3C32-4D03-B9E6-14CF56F7458D@pbrc .edu> Message-ID: <1289834659l.966764l.0l@psu.edu> Thanks Tina. I will keep in mind the thickness for staining purposes that is once the slicing protocol gets optimized. So we use 200-300 ml of both PBS and PFA for our perfusion. So the post fix and sucrose treatment you are suggesting, are you suggesting for the new harvests alone which I will be doing or the existing frozen perfused ones? Anjum On Sun, Nov 14, 2010 10:35 PM, "Montina Van Meter" wrote: > Anhum, >How much PBS and Paraformaldehyde do you put through the (rat or >mouse). I flush a perfuse our rats with 150-200ml. of PBS and 500ml. >of Para. Postfix for 2 hours and place in 20% sucrose overnight until >the tisue sinks to the bottom of the vial. >The morphology of your tissue is going to be compromised due to freeze >artifact (lack of cryoprotectant) and there will be holes in your >sections. > >Actually, 30um thick sections are considered quite thick and are >typically used for free floating techniques. Sections that are mounted >on slides before IHC staining are much thinner (3-10um). I usually >cut my tissue between 30-40um and manually free float the sections for >IHC or IF. > >Tina > > >Sent from my iPhone > >On Nov 14, 2010, at 9:05 PM, "ANJUM PARKAR" >wrote: > >> Thanks Tina. I will keep the sucrose suggestion in mind for future >> harvests. >> For now I need to figure out how to slice the already fixed frozen >> tissue. Will >> try to equilibrate in the cryostat itself as against room >> temperature and see >> how that goes. The knife angle is 13 degrees and recently sharpened >> and >> thickness of slices 30 microns which I believe is standard for >> sectioning. I >> have sectioned previously a lot so doing hands on is something I am >> familiar >> with. But I did realize every instrument is different and while >> doing a >> procedure it always needs to be optimized until it can become >> routine. Will let >> you know how things go. >> Anjum >> >> >> On Sun, Nov 14, 2010 02:21 PM, "Montina Van Meter" >> > >> wrote: >> >> Anhum, >> 1. You MUST cryoprotect the fixed tissue in 15-30% sucrose (until it >> sinks) prior to cutting frozen sections. >> 2. Allow the -80C embedded tissue block to equilabrate to -20C in the >> cryostat for 15-20min. >> 3. Check the knife angle. Thickness? >> 4. Change knife or sharpen and make sure all screws are tight. >> 5. Freez/thawing of block is not a good idea (especially since it's >> not cryoprotected). You are introducing ice crystal artifact. >> 6. Check around your department (or Histology Core) to see if >someone >> >> can instruct you on using the cryostat. It's always better to >> actually >> watch someone in addition to receiving written instructions. >> >> Good luck, >> Tina >> >> >> Sent from my iPhone >> >> On Nov 14, 2010, at 10:47 AM, "ANJUM PARKAR" > >> wrote: >> >> Hello all, >> I have been having some histology issues in terms of rat brain >> slicing the past >> few months and hence looking for suggestions to fix them, having >> tried most of >> what I know from my past training and running of ideas real fast >> now. >> The following is the protocol I have used thus far:-1. Animal >> perfusion-a)Use >> PBS first, b) Use 4%PFA next and c) Harvest brain right after >> PFA. >> (Transcardial perfusion)2. Freeze brain by embedding in OCT >> compound >> using dry >> ice and then storing at -80 until ready to slice. Alternatively some >> times the >> -80 is used straight up with out dry ice freezing (still embedded in >> OCT).3. >> From standard protocol I do not do two things-a) No post fixation >> once brain is >> harvested and b) No sucrose. Reason being, I was taught that these >> two steps >> might result in large holes in tissue.4.For slicing, we have the >> older cryostat >> version (Real old version of CM3050S from Vibrotome-30 micron >> slices) which >> uses a fixed knife for slicing and has a chamber temp control and >> one for the >> specimen. The cryostat has not been serviced for a few years now.5. >> In attempt >> 1-I take the brain out of the -80 once the chamber temperature comes >> to -20 and >> mount the frozen mold on the chuck and attempt at slicing. Doing >> this I managed >> to get a few slices (that too with cuts and ripples) and after >> which >> the tissue >> just kept cracking and falling into pieces and hence could get no >> more >> slices.6. In attempt 2-I thought the brain may be too cold and too >> hard >> (perfusion and-80 freezing), so I defrosted the brain for 20 >> mins at >> room >> temperature before sticking into -20 cryostat. Between slices if I >> felt the >> tissue was too cold, I put my thumb on the specimen to warm it up. >> Still no >> luck.7. In attempt 3-I defrosted the brain completely to room >> temperature, took >> the brain out of the cryogel embedding (thinking that the mold was >> way too hard >> to cut through and hence cracking the tissue too) and attached it to >> chuck >> directly and tried slicing. Still no luck at all this time around. >> From what I have been reading online and from what I know, I >> primarily feel >> these are issues related with temperature of the brain and that it >> is too cold >> during cutting. I have tried different ways to bring the temperature >> down and I >> still have no success. I have eight other brains frozen using the >> protocol >> above and I really need to get slices out of them. Do you have any >> suggestions >> for me? Also do you think I should make any changes to the existing >> freezing >> protocol for the future brain harvesting? >> APPenn State University,PA >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- Anjum Parkar Doctoral Candidate Department of Bioengineering, Penn State University, 206 Hallowell Building, University Park, PA 16802. From laurie.colbert <@t> huntingtonhospital.com Mon Nov 15 09:28:35 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Nov 15 09:28:47 2010 Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) In-Reply-To: Message-ID: <57BE698966D5C54EAE8612E8941D768309E91CE9@EXCHANGE3.huntingtonhospital.com> We are using both. They both have had their fair share of problems, but overall I think the improved efficiency is worth working out the kinks. I recommend both. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Damaris Beil Sent: Saturday, November 13, 2010 6:42 AM To: histonet Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) Hello, I'm interested in finding out if anyone is using ThermoFisher's new PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) and how they are working out for you. Thanks in advance for your help, Damaris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> uropartners.com Mon Nov 15 10:21:10 2010 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Mon Nov 15 10:21:15 2010 Subject: [Histonet] Annual Review of Manuals Message-ID: For those of you who work in/manage CAP accredited laboratories, what is your current understanding/policy in regards to annual review of all relevant procedure manuals by all personnel? If you no longer do this, what procedure manual review IS done in your lab? Thanks, Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 From newenglandurology <@t> gmail.com Mon Nov 15 10:28:35 2010 From: newenglandurology <@t> gmail.com (Johannes Koepplinger) Date: Mon Nov 15 10:28:40 2010 Subject: [Histonet] prostate biopsies Message-ID: Can anyone share their processing schedules for prostate biopsies using a variety of processors? Examples: Sakura VIP, Tissue Tek express, Leica Peloris, or Thermo Pathcentre? What is the optimum rapid processing schedule? Do you prefer to use a certain kind of alcohol? From Bill.Tench <@t> pph.org Mon Nov 15 10:30:56 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Mon Nov 15 10:31:10 2010 Subject: [Histonet] Procedure manual review Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5816@MAIL1.pph.local> All procedures must be reviewed annually (and that means WITHIN 12 months, not just once a calender year) This may be done by the appropriate supervisor or pathologist, not necessarily the Director (Director must review all new or changed procedures) The review should confirm that the procedure in operation matches what really happens. The laboratory must have in place a mechanism that demonstrates that the users of those procedures are familiar with them (and without digging up the CAP standards, i rely on my memory that this must also be documented in some format on an annual basis). I believe we accomplish this with an annual signed statement from the employees confirming they are familiar with the procedures they use. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From Sandra.Harrison3 <@t> va.gov Mon Nov 15 10:32:58 2010 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Mon Nov 15 10:33:09 2010 Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner) In-Reply-To: <57BE698966D5C54EAE8612E8941D768309E91CE9@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768309E91CE9@EXCHANGE3.huntingtonhospital.com> Message-ID: I'm looking at the Slide Mate and Print Mate, too. Currently, I have the Leica cassette and slide printers. They have really been great. Reliable and relatively problem free. But they are so large. I'd like to find a slide printer that has a small footprint, like the Slide Mate, so that we can place one at each microtomy station. Assuming there are other slide printers out there, besides ThermoFisher's Slide Mate and Leica's, what are their pro's and con's? Sandy Harrison -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Monday, November 15, 2010 9:29 AM To: Damaris Beil; histonet Subject: RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner) We are using both. They both have had their fair share of problems, but overall I think the improved efficiency is worth working out the kinks. I recommend both. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Damaris Beil Sent: Saturday, November 13, 2010 6:42 AM To: histonet Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) Hello, I'm interested in finding out if anyone is using ThermoFisher's new PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) and how they are working out for you. Thanks in advance for your help, Damaris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vavalos <@t> allergydermatology.com Mon Nov 15 11:50:50 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Mon Nov 15 11:50:56 2010 Subject: [Histonet] Tap Water or filtration system Message-ID: <000e01cb84ed$a49cdbd0$edd69370$@com> We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain H&E at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 From ejschmid <@t> ucalgary.ca Mon Nov 15 12:01:23 2010 From: ejschmid <@t> ucalgary.ca (ejschmid@ucalgary.ca) Date: Mon Nov 15 12:01:35 2010 Subject: [Histonet] glutaraldehyde fixed tissues and genomic DNA Message-ID: <4d3d55b1e538069a91a893b7a2046f7f.squirrel@webmail.ucalgary.ca> Hello, I have PFA+glutaraldehyde fixed tissues that I might want to digest down in order to extract genomic DNA suitable for bisulfite sequencing. The tissues are embryonic mouse heads. The fixative is a PFA + glutaraldehyde mix: 3.6 PFA with 5% glut. The glut is grade 1 or electron miccroscopy grade. I'd like to pro-K the tissues and then phenol-chloroform extract genomic DNA. My hope is that the amount and quality of the DNA will be suitable for bisulfite conversions for methylation anaylsis. Will the DNA be abundant? Will it be fairly protein free (does the fixative fix protein to the DNA?). Thanks Eric University of Calgary Facutly of Medicine From jmcmillan <@t> ccpathology.com Mon Nov 15 12:09:30 2010 From: jmcmillan <@t> ccpathology.com (Jaime McMillan) Date: Mon Nov 15 12:09:39 2010 Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner) In-Reply-To: <57BE698966D5C54EAE8612E8941D768309E91CE9@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768309E91CE9@EXCHANGE3.huntingtonhospital.com> Message-ID: I agree with Laurie. We have seen a great improvement in efficiency and reduction of errors with this hardware. We have been using both for a few months now and have not had many problems. Jaime McMillan -----Original Message----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Monday, November 15, 2010 7:29 AM To: Damaris Beil; histonet Subject: RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner) We are using both. They both have had their fair share of problems, but overall I think the improved efficiency is worth working out the kinks. I recommend both. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Damaris Beil Sent: Saturday, November 13, 2010 6:42 AM To: histonet Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) Hello, I'm interested in finding out if anyone is using ThermoFisher's new PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) and how they are working out for you. Thanks in advance for your help, Damaris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mfisher <@t> ecrmc.org Mon Nov 15 12:12:34 2010 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Mon Nov 15 12:12:44 2010 Subject: [Histonet] Non-Refrigerated RPMI/3-in-1 Pap Stain Message-ID: <3ACBB5D73A417547A01970CD3EB55093047B63CB@MAIL1.ecrmc.ci.el-centro.ca.us> I would like to obtain information to order the non-refrigerated RPMI. And does a 3-in-one Pap stain exist? Someone in a workshop in Seattle said they knew about it but couldn't give the exact supplier. Thank you for your help. M. Fisher El Centro Regional Medical Center www.ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? From lblazek <@t> digestivespecialists.com Mon Nov 15 12:15:08 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Nov 15 12:15:15 2010 Subject: [Histonet] Tap Water or filtration system In-Reply-To: <000e01cb84ed$a49cdbd0$edd69370$@com> References: <000e01cb84ed$a49cdbd0$edd69370$@com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EB42CE431@IBMB7Exchange.digestivespecialists.com> I don't have the Lecia Autostainer but have a Surgipath Tribune stainer. I have it connected to tap water and do both H&E and PAS on it. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Monday, November 15, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tap Water or filtration system We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain H&E at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Mon Nov 15 12:22:23 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Nov 15 12:22:33 2010 Subject: [Histonet] Tap Water or filtration system In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EB42CE431@IBMB7Exchange.digestivespecialists.com> References: <000e01cb84ed$a49cdbd0$edd69370$@com> <5A2BD13465E061429D6455C8D6B40E390EB42CE431@IBMB7Exchange.digestivespecialists.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044A072253@CHEXCMS10.one.ads.che.org> We use tap water on our Leica. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, November 15, 2010 13:15 To: 'Vanessa Avalos'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tap Water or filtration system I don't have the Lecia Autostainer but have a Surgipath Tribune stainer. I have it connected to tap water and do both H&E and PAS on it. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Monday, November 15, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tap Water or filtration system We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain H&E at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Rick.Garnhart <@t> memorialhealthsystem.com Mon Nov 15 12:57:18 2010 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Mon Nov 15 12:57:30 2010 Subject: [Histonet] Slide Mates and Cassette printers In-Reply-To: Message-ID: We have three of the older six hopper Thermo Micro cassette writers and one Thermo slide mate, in addition to four of the Pslims. The Pslims are the first generation Slide Mate by Accuplace. They are all connected to our APLIS System (PowerPath) and we use 2D bar-codes on our specimen labels, cassettes and slides. We do not produce a cassette or slide without wanding a bar-code. The printed specimen bar codes that generate the cassettes and bar-coded cassettes that generate the slides for positive patient identification, out weighs the manual human method is well worth the effort. This is where all histology lab should be moving towards. We have some little network issue with them, but for the most part they print great. The cassette printers work well. Remember these are mechanical and computer driven, so they will fail at times. Please feel free to contact me if you have any questions. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From JMacDonald <@t> mtsac.edu Mon Nov 15 13:51:56 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Nov 15 13:52:09 2010 Subject: [Histonet] Tap Water or filtration system In-Reply-To: <000e01cb84ed$a49cdbd0$edd69370$@com> Message-ID: We always used tap water, without any problems. "Vanessa Avalos" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/15/2010 09:54 AM To cc Subject [Histonet] Tap Water or filtration system We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain H&E at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From newenglandurology <@t> gmail.com Mon Nov 15 14:19:31 2010 From: newenglandurology <@t> gmail.com (Johannes Koepplinger) Date: Mon Nov 15 14:19:34 2010 Subject: [Histonet] pathologists Message-ID: Would any of the pathologists or histotechs know what the average volume of work for a urology pathologist would be for a given day? How many slides/cases is a comfortable workload for one day of reading, say in regard to prostate biopsies or bladder biopsy cases (excluding vas deferens)? Also including immunos if any. From akinlab <@t> txplan.com Mon Nov 15 14:52:54 2010 From: akinlab <@t> txplan.com (akinlab@txplan.com) Date: Mon Nov 15 14:54:05 2010 Subject: [Histonet] IHC & Mohs Message-ID: <3173cf9a$1d11cba0$5f338d76$@com> Hi Everyone!! I need advise on getting started with IHC on Mohs sections! Vendors, automated vs by hand, antibodies....anything and everything will be soooo greatly appreciated. Please and Thank You in advance!! From NMargaryan <@t> childrensmemorial.org Mon Nov 15 15:20:33 2010 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Nov 15 15:21:32 2010 Subject: [Histonet] RE:Tap Water or filtration system In-Reply-To: References: Message-ID: What if we suggest to use ddH2O both for TBST preparation and in autostainers (before and after IHC) because the PH level is vary in different states. Naira ************** Confidentiality Note: This message (including any attachments) is intended for the use of the named recipient(s) only and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. -----Original Message----- Message: 11 Date: Mon, 15 Nov 2010 10:50:50 -0700 From: "Vanessa Avalos" Subject: [Histonet] Tap Water or filtration system To: Message-ID: <000e01cb84ed$a49cdbd0$edd69370$@com> Content-Type: text/plain; charset="us-ascii" We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain H&E at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 From TroyerDA <@t> EVMS.EDU Mon Nov 15 15:25:36 2010 From: TroyerDA <@t> EVMS.EDU (Troyer, Dean A.) Date: Mon Nov 15 15:25:56 2010 Subject: [Histonet] pathologists References: Message-ID: <71EE6DB05CB563458E8CBA495B3E4BD003F56187@romulus.evms.net> It is important to understand details of slide preparation. We prepare six levels of each block/ each glass slide. A case is prostate biopsies from one patient. 85% of the time we get two biopsy cores per container and thus two cores per block, and 12 cores total per case. A busy day is 12 biopsy cases, 6 slides each, with six levels of each block (containing two biopsy cores) on each slide). I am aware that some pathologists deal with as many as 20 prostate biopsy cases per day. The detail of how the slides are prepared and screened can differ from place to place. The experience of the pathologist also affects throughput. The only measure we really have of QA is prevalence (i.e. are we missing cases). Prevalence of prostate cancer in cases varies surprisingly ranging from high 20s to high 45%. I've been in two different sites in which 45-50% of cases are positive. The patient population being screened and biopsied obviously would impact the prevalence rate. Dean Troyer ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Johannes Koepplinger Sent: Mon 11/15/2010 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pathologists Would any of the pathologists or histotechs know what the average volume of work for a urology pathologist would be for a given day? How many slides/cases is a comfortable workload for one day of reading, say in regard to prostate biopsies or bladder biopsy cases (excluding vas deferens)? Also including immunos if any. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Mon Nov 15 15:25:57 2010 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Mon Nov 15 15:26:11 2010 Subject: [Histonet] Control sections for frozen sections Message-ID: Does anyone run a H&E control prior to staining a frozen section? Diana From AnthonyH <@t> chw.edu.au Mon Nov 15 16:03:29 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Nov 15 16:03:42 2010 Subject: [Histonet] Tap Water or filtration system In-Reply-To: <000e01cb84ed$a49cdbd0$edd69370$@com> Message-ID: We use tap water and have had no problems. Sydney tap water seems to be quite innocuous Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Tuesday, 16 November 2010 4:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tap Water or filtration system We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain H&E at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From sfeher <@t> CMC-NH.ORG Mon Nov 15 16:45:24 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Nov 15 16:45:31 2010 Subject: [Histonet] Tap Water or filtration system In-Reply-To: <000e01cb84ed$a49cdbd0$edd69370$@com> References: <000e01cb84ed$a49cdbd0$edd69370$@com> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC243867FD@exchange.cmc-nh.org> We use tap water in our Leica multistainer for H&E's and a few other stains. The protocols can be optimized to accommodate what ever you are using. I can't speak to the PAS stain except to say that even if you need deionized water, you can buy it in 5 gallon cubes for limited use and still save money. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Monday, November 15, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tap Water or filtration system We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain H&E at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Mon Nov 15 17:41:04 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Nov 15 17:41:47 2010 Subject: [Histonet] Cardboard slide flat storage In-Reply-To: <931675.17966.qm@web113214.mail.gq1.yahoo.com> References: <931675.17966.qm@web113214.mail.gq1.yahoo.com> Message-ID: Hi Meghan, We use grey colored boxes that will hold either 8 cardboard "drawers" of blocks or 4 cardboard "drawers" of slides. I am at home right now, & don't have the specific ordering information, but will share it tomorrow when I get to work. I just didn't want to let the question languish w/o some sort of response! Michelle On Nov 15, 2010, at 9:30 AM, Meghan Tucker wrote: > Good morning, > > Are there any suggestions on a place to order a storage shelf for cardboard slide flats, which may have 6-8 'cubbies' that can be used to organize the flats? > > Thanks! > > Meghan > > Meghan.Tucker@yahoo.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MSHERWOOD <@t> PARTNERS.ORG Tue Nov 16 07:19:47 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Nov 16 07:19:51 2010 Subject: [Histonet] Refurbished histology Leica equipment In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB51F8@PHSXMB30.partners.org> We have the Leica XL autostainer and CV 5030 coverslipper, both refurbished units, and are very happy with them. We saved a lot of money by doing so. We purchased both from Medical Equipment Source. We also purchased a Leica RM2255 automatic microtome as well. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Damaris Beil Sent: Friday, November 12, 2010 6:54 PM To: histonet Subject: [Histonet] Refurbished histology Leica equipment Hello, I would like to know if anyone has had any experience using a refurbished Leica XL auto stainer and coverslip CV5000. Any thoughts you would not mind sharing? Thanks in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From MSHERWOOD <@t> PARTNERS.ORG Tue Nov 16 07:22:48 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Nov 16 07:22:53 2010 Subject: [Histonet] Tap Water or filtration system In-Reply-To: <000e01cb84ed$a49cdbd0$edd69370$@com> References: <000e01cb84ed$a49cdbd0$edd69370$@com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB51F9@PHSXMB30.partners.org> We also use tap water, but have a filter unit attached. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Monday, November 15, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tap Water or filtration system We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain H&E at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From lmayhew5 <@t> cogeco.ca Tue Nov 16 07:43:18 2010 From: lmayhew5 <@t> cogeco.ca (Lee Mayhew) Date: Tue Nov 16 07:43:26 2010 Subject: [Histonet] Control sections for frozen sections Message-ID: <7C1E352C-BA90-4370-A47E-8DFD19DCF5FC@cogeco.ca> Hi Diana, We run one frozen section H&E every morning after the frozen section staining reagents have been filtered, changed or topped up. The QC slide is checked by the technologist and the results recorded and initialled on the QC sheet. Lee Hamilton, Ontario From akemiat3377 <@t> yahoo.com Tue Nov 16 08:20:38 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue Nov 16 08:20:47 2010 Subject: [Histonet] Thank you Ventana Symphony Message-ID: I would like to thank all of you that sent me information regarding the Ventana Symphony. It appears from the dozen of you that sent me information, you were all pretty consistent in your assessments. It was exactly what I needed and I now feel confident to attend my meeting. Thanks a bunch! Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From axp969 <@t> psu.edu Tue Nov 16 09:54:39 2010 From: axp969 <@t> psu.edu (ANJUM PARKAR) Date: Tue Nov 16 09:54:46 2010 Subject: [Histonet] neurodegeneration stains? Message-ID: <1289922879l.1323230l.0l@psu.edu> Hi all, I am trying to characterize neuronal degeneration and was wondering if there are any fluorescent markers out there to stain for dead/dying neurons? I have used Silver stain and am currently trying to optimize the Fluro Jade C marker too. But I need some more and the literature seems limited on this. Thanks -- Anjum From histotech <@t> imagesbyhopper.com Tue Nov 16 10:42:42 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Nov 16 10:42:51 2010 Subject: [Histonet] Cardboard slide flat storage In-Reply-To: Message-ID: <30ACBC309D5C4373B3866961CD89EE30@hopperPC> Meghan, This is the catalog number for the slide/block storage boxes we use: 22-272-957 and we get them from Fisher Scientific. The following is the link on fisher's website: http://www.fishersci.com/wps/portal/PRODUCTDETAIL?prodcutdetail='prod'&produ ctId=1589085&catalogId=29104&matchedCatNo=22272957&pos=1&catCode=RE_SC&endec aSearchQuery=%23store%3DScientific%23N%3D0%23rpp%3D15&fromCat=yes&keepSessio nSearchOutPut=true&fromSearch=Y&searchKey=272957||22&highlightProductsItemsF lag=Y Hope that helps! Michelle __________________________________________________ Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cardboard slide flat storage Hi Meghan, We use grey colored boxes that will hold either 8 cardboard "drawers" of blocks or 4 cardboard "drawers" of slides. I am at home right now, & don't have the specific ordering information, but will share it tomorrow when I get to work. I just didn't want to let the question languish w/o some sort of response! Michelle On Nov 15, 2010, at 9:30 AM, Meghan Tucker wrote: > Good morning, > > Are there any suggestions on a place to order a storage shelf for > cardboard slide flats, which may have 6-8 'cubbies' that can be used to organize the flats? > > Thanks! > > Meghan > > Meghan.Tucker@yahoo.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1153 / Virus Database: 424/3260 - Release Date: 11/16/10 From histotech <@t> imagesbyhopper.com Tue Nov 16 11:09:16 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Nov 16 11:09:31 2010 Subject: [Histonet] Partially muddy slides In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB51F9@PHSXMB30.partners.org> Message-ID: Hi Histonetters! We are having an issue in our lab with some inconsistent staining. It's an odd situation. One small biopsy can stain reasonably well over most of the tissue, but then one area of it is muddy. This does not affect all the slides in the same staining rack, nor does it affect all the small biopsies that were processed in the same processing cycle. Troubleshooting this has been difficult, at best. It's sort of hit or miss on when/where the muddy-ness appears. Here are my thoughts: ** I don't know if this is a processing issue, a staining issue or perhaps even a collection issue. ** If it affected all small biopsies equally, I would be tempted to question the processing/fixation. But it doesn't affect across the board. ** If all the staining was muddy, I would look to the stainer, but again, even within the same slide, we can have clear and muddy areas. ** The stainer is a Leica XL (about 3 years old), hooked up to tap water for the washes. ** We are using a regressive staining method with Fisher brand Protocol Harris hematoxylin and Protocol Eosin Y. We use acid alcohol and ammonia water for differentiating and bluing. ** The xylene we use is recycled, but verified for purity. ** The alcohols are recycled, but the last alcohol prior to the xylene/coverslipping is "pure", not recycled (for just in case). Suggestions on what could be causing this issue would be greatly appreciated. Michelle From Clough <@t> medicine.tamhsc.edu Tue Nov 16 11:09:38 2010 From: Clough <@t> medicine.tamhsc.edu (Clough, Bret) Date: Tue Nov 16 11:09:44 2010 Subject: [Histonet] Disposable blade holder for a Leica 1512 rotary microtome. Message-ID: I hope that someone on histonet could help me. Our research lab just acquired an old Leica 1512 in good working order but lacking a blade holder. Is it possible for us to find a blade holder for this machine or is this a futile cause? Any information you all could provide would be greatly appreciated. Thanks, Bret Clough Texas A&M Health Science Center Temple TX. From liz <@t> premierlab.com Tue Nov 16 11:15:22 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Nov 16 11:12:44 2010 Subject: [Histonet] neurodegeneration stains? In-Reply-To: <1289922879l.1323230l.0l@psu.edu> Message-ID: Anjum Those are the two that I'm familiar with. I'm not aware of any others. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ANJUM PARKAR Sent: Tuesday, November 16, 2010 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] neurodegeneration stains? Hi all, I am trying to characterize neuronal degeneration and was wondering if there are any fluorescent markers out there to stain for dead/dying neurons? I have used Silver stain and am currently trying to optimize the Fluro Jade C marker too. But I need some more and the literature seems limited on this. Thanks -- Anjum _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Tue Nov 16 11:49:57 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Nov 16 11:50:18 2010 Subject: [Histonet] Partially muddy slides In-Reply-To: <4CE2BBD2.8090905@pathology.washington.edu> Message-ID: <993B8EBDB9494E8C8C60DCD70D39F083@hopperPC> Regarding incomplete deparaffinizing: I did give that some thought, but wouldn't that affect ALL the slides and not just hit or miss? It's primarily the small biopsies that are being affected, and I would think those would clear the paraffin much easier than the big specimens? Just as a troubleshooting step, I will increase the deparaffinzing times in the stainer. Jeff, I wondered if the collecting nurse/doctor was possibly placing the tissue in saline prior to formalin? A cautery effect... Would that show up as muddy or more burned/desiccated? Please keep the ideas coming! Michelle -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Tuesday, November 16, 2010 12:14 PM To: histotech@imagesbyhopper.com Subject: Re: [Histonet] Partially muddy slides What about incomplete paraffin removal? Without seeing the slides it is hard to tell. Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 11/16/2010 9:09 AM, histotech@imagesbyhopper.com wrote: > Hi Histonetters! > > We are having an issue in our lab with some inconsistent staining. > It's an odd situation. One small biopsy can stain reasonably well > over most of the tissue, but then one area of it is muddy. This does > not affect all the slides in the same staining rack, nor does it > affect all the small biopsies that were processed in the same > processing cycle. Troubleshooting this has been difficult, at best. > It's sort of hit or miss on when/where the muddy-ness appears. > > Here are my thoughts: > ** I don't know if this is a processing issue, a staining issue or > perhaps even a collection issue. > ** If it affected all small biopsies equally, I would be tempted to > question the processing/fixation. But it doesn't affect across the > board. > ** If all the staining was muddy, I would look to the stainer, but again, > even within the same slide, we can have clear and muddy areas. > ** The stainer is a Leica XL (about 3 years old), hooked up to tap water > for the washes. > ** We are using a regressive staining method with Fisher brand Protocol > Harris hematoxylin and Protocol Eosin Y. We use acid alcohol and ammonia > water for differentiating and bluing. > ** The xylene we use is recycled, but verified for purity. > ** The alcohols are recycled, but the last alcohol prior to the > xylene/coverslipping is "pure", not recycled (for just in case). > > Suggestions on what could be causing this issue would be greatly > appreciated. > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1153 / Virus Database: 424/3260 - Release Date: 11/16/10 From heather.mcleod <@t> webmail.co.za Tue Nov 16 12:34:39 2010 From: heather.mcleod <@t> webmail.co.za (heather.mcleod@webmail.co.za) Date: Tue Nov 16 12:34:45 2010 Subject: [Histonet] connexin 43 ab Message-ID: <0a1d8a0109504dc900e9fd156e2abc6c@fr.webmail.co.za> Dear Histonetters Does anybody have a preference for a connexin-43 ab? A registrar needs it for a research project. I am leaning towards the Sigma C6219 rabbit plyclonal. I would appreciate any suggestions. The material would be ffpe human heart tissue Many thanks Heather McLeod ____________________________________________________________ South Africas premier free email service - www.webmail.co.za Save on insurance with OUTsurance https://www.outsurance.co.za/insurance-quote/?source=webmailmailer&cr=thou10_468x60&cid=37 From wlecorch <@t> rwjuhh.edu Tue Nov 16 12:38:47 2010 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Tue Nov 16 12:38:56 2010 Subject: [Histonet] Vol 84, Issue 20 Non-Refrigerated RPMI/3-in-1 Pap Stain Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71B73163F9B@HAMEXMBA.rwjham.local> For those who were in Seattle this is the info you are looking for ... The RPMI storage temp is +15 C to + 30 C and we purchase ours from Fisher (cat# 12-167F Lonza BioWhittaker RPMI-1640 500ml www.fishersci.com The 3-in-1 Pap is called EasyPap stain and we purchase ours from Azer scientific cat# ES7037A www.AzerSci.com Bill Lecorchick Cytology Prep.Tech. 609-584-5128 Fax 609-584-6439 wlecorch@rwjuhh.edu www.rwjhamilton.org From Wanda.Smith <@t> HCAhealthcare.com Tue Nov 16 13:07:28 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Tue Nov 16 13:07:35 2010 Subject: [Histonet] FW: What % of IHC Stains Should You Expect to Repeat? Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1396DEF84E@NADCWPMSGCMS03.hca.corpad.net> WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _____________________________________________ From: Smith Wanda Sent: Tuesday, November 16, 2010 1:59 PM To: 'histonet-request@lists.utsouthwestern.edu' Subject: What % of IHC Stains Should You Expect to Repeat? Good Afternoon, I am having an issue with my Pathologist who thinks when the patient tissue on an IHC stain doesn't "look like what he thought it should look like", he then wants to stop doing Immunos and send everything out!!!! This may happen about twice a month, but the control always stains beautifully. We recently had the stainer serviced and the drop zone was adjusted to alleviate any problems with distribution of the solutions. On a recent test slide of a new control block of colon cancer, he also didn't like that CD X2 did not stain some of the tissue components, but the rest of the tissue on the test slide stained beautifully. Please advise me on what to say to this Pathologist. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From JMacDonald <@t> mtsac.edu Tue Nov 16 13:18:36 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Nov 16 13:18:41 2010 Subject: [Histonet] Partially muddy slides In-Reply-To: Message-ID: Is it the same person cutting all of the problem biopsies? Does your lab use cryo spray? We had biopsies that had a muddy/freezer burnt appearance. We traced all of the problems to one histotech who used the cryo spray excessively. Sent by: histonet-bounces@lists.utsouthwestern.edu 11/16/2010 09:12 AM To cc Subject [Histonet] Partially muddy slides Hi Histonetters! We are having an issue in our lab with some inconsistent staining. It's an odd situation. One small biopsy can stain reasonably well over most of the tissue, but then one area of it is muddy. This does not affect all the slides in the same staining rack, nor does it affect all the small biopsies that were processed in the same processing cycle. Troubleshooting this has been difficult, at best. It's sort of hit or miss on when/where the muddy-ness appears. Here are my thoughts: ** I don't know if this is a processing issue, a staining issue or perhaps even a collection issue. ** If it affected all small biopsies equally, I would be tempted to question the processing/fixation. But it doesn't affect across the board. ** If all the staining was muddy, I would look to the stainer, but again, even within the same slide, we can have clear and muddy areas. ** The stainer is a Leica XL (about 3 years old), hooked up to tap water for the washes. ** We are using a regressive staining method with Fisher brand Protocol Harris hematoxylin and Protocol Eosin Y. We use acid alcohol and ammonia water for differentiating and bluing. ** The xylene we use is recycled, but verified for purity. ** The alcohols are recycled, but the last alcohol prior to the xylene/coverslipping is "pure", not recycled (for just in case). Suggestions on what could be causing this issue would be greatly appreciated. Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vjp2105 <@t> columbia.edu Tue Nov 16 13:19:26 2010 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Tue Nov 16 13:19:33 2010 Subject: [Histonet] =?iso-8859-1?q?Biocare=B9s_Rodent_Decloaking_Solution?= Message-ID: Hi everyone, For anyone that uses Biocare's Rodent Decloaking Solution, once the solution has been made up, can it be reused? And if so in your experience how many times? Many thanks in advance From christiegowan <@t> msn.com Tue Nov 16 13:22:21 2010 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Tue Nov 16 13:22:27 2010 Subject: [Histonet] FW: What % of IHC Stains Should You Expect to Repeat? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1396DEF84E@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1396DEF84E@NADCWPMSGCMS03.hca.corpad.net> Message-ID: Send the colon cancer control out and compare your results to the send out. If they are the same then you have an argument. > From: Wanda.Smith@HCAhealthcare.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 16 Nov 2010 13:07:28 -0600 > Subject: [Histonet] FW: What % of IHC Stains Should You Expect to Repeat? > > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > > _____________________________________________ > From: Smith Wanda > Sent: Tuesday, November 16, 2010 1:59 PM > To: 'histonet-request@lists.utsouthwestern.edu' > Subject: What % of IHC Stains Should You Expect to Repeat? > > > Good Afternoon, > I am having an issue with my Pathologist who thinks when the patient tissue on an IHC stain doesn't "look like what he thought it should look like", he then wants to stop doing Immunos and send everything out!!!! This may happen about twice a month, but the control always stains beautifully. We recently had the stainer serviced and the drop zone was adjusted to alleviate any problems with distribution of the solutions. On a recent test slide of a new control block of colon cancer, he also didn't like that CD X2 did not stain some of the tissue components, but the rest of the tissue on the test slide stained beautifully. Please advise me on what to say to this Pathologist. > Thanks, > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Nov 16 14:30:39 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Nov 16 14:30:46 2010 Subject: [Histonet] Disposable blade holder for a Leica 1512 rotary microtome. In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044A07250F@CHEXCMS10.one.ads.che.org> Klaus Dern at 706-635-8840 will make you one that works like a regular knife holding a disposable blade. I copied everyone in case someone else needs the info. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clough, Bret Sent: Tuesday, November 16, 2010 12:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposable blade holder for a Leica 1512 rotary microtome. I hope that someone on histonet could help me. Our research lab just acquired an old Leica 1512 in good working order but lacking a blade holder. Is it possible for us to find a blade holder for this machine or is this a futile cause? Any information you all could provide would be greatly appreciated. Thanks, Bret Clough Texas A&M Health Science Center Temple TX. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rjbuesa <@t> yahoo.com Tue Nov 16 16:28:28 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 16 16:28:34 2010 Subject: [Histonet] FW: What % of IHC Stains Should You Expect to Repeat? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1396DEF84E@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <17000.86338.qm@web65706.mail.ac4.yahoo.com> That type of "pathologist" deserves an answer that could cost you your job, so it is not advisable to do that. That type of "pathologist" is essentially ignorant of what we do and do not reason. If he wants to send everything out, let him. When charges begin to hit your administration, perhaps he will be more open to reasoning. Ren? J. --- On Tue, 11/16/10, Wanda.Smith@HCAhealthcare.com wrote: From: Wanda.Smith@HCAhealthcare.com Subject: [Histonet] FW: What % of IHC Stains Should You Expect to Repeat? To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 16, 2010, 2:07 PM WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _____________________________________________ From: Smith Wanda Sent: Tuesday, November 16, 2010 1:59 PM To: 'histonet-request@lists.utsouthwestern.edu' Subject: What % of IHC Stains Should You Expect to Repeat? Good Afternoon, I am having an issue with my Pathologist who thinks when the patient tissue on an IHC stain doesn't "look like what he thought it should look like", he then wants to stop doing Immunos and send everything out!!!!? This may happen about twice a month, but the control always stains beautifully.? We recently had the stainer serviced and the drop zone was adjusted to alleviate any problems with distribution of the solutions.? On a recent test slide of a new control block of colon cancer, he also didn't like that CD X2 did not stain some of the tissue components, but the rest of the tissue on the test slide stained beautifully.? Please advise me on what to say to this Pathologist. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Nov 16 17:23:53 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Nov 16 17:24:05 2010 Subject: [Histonet] Partially muddy slides In-Reply-To: Message-ID: Michelle, It might possible be water contamination in the xylene. Try increasing alcohol dehydration times. Also re-cyled alcohol is probably around 96% pure. It seems to be not possible to obtain absolute ethanol by re-cycling, or so I am lead to believe. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Wednesday, 17 November 2010 4:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Partially muddy slides Hi Histonetters! We are having an issue in our lab with some inconsistent staining. It's an odd situation. One small biopsy can stain reasonably well over most of the tissue, but then one area of it is muddy. This does not affect all the slides in the same staining rack, nor does it affect all the small biopsies that were processed in the same processing cycle. Troubleshooting this has been difficult, at best. It's sort of hit or miss on when/where the muddy-ness appears. Here are my thoughts: ** I don't know if this is a processing issue, a staining issue or perhaps even a collection issue. ** If it affected all small biopsies equally, I would be tempted to question the processing/fixation. But it doesn't affect across the board. ** If all the staining was muddy, I would look to the stainer, but again, even within the same slide, we can have clear and muddy areas. ** The stainer is a Leica XL (about 3 years old), hooked up to tap water for the washes. ** We are using a regressive staining method with Fisher brand Protocol Harris hematoxylin and Protocol Eosin Y. We use acid alcohol and ammonia water for differentiating and bluing. ** The xylene we use is recycled, but verified for purity. ** The alcohols are recycled, but the last alcohol prior to the xylene/coverslipping is "pure", not recycled (for just in case). Suggestions on what could be causing this issue would be greatly appreciated. Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From axp969 <@t> psu.edu Tue Nov 16 17:55:27 2010 From: axp969 <@t> psu.edu (ANJUM PARKAR) Date: Tue Nov 16 17:55:33 2010 Subject: [Histonet] Frozen brain slices In-Reply-To: 4FE7FB862E90E448AE32388E759220E502ACE344@pbrcas31.pbrc.edu References: <1289752737l.1429610l.0l@psu.edu><50809B73-F3A5-41E8-A42E-23ED6268A2E5@pbrc .edu><1289789628l.1421536l.0l@psu.edu><53DBCC77-3C32-4D03-B9E6-14CF56F7458D @pbrc.edu> <1289834659l.966764l.0l@psu.edu><4FE7FB862E90E448AE32388E759220E502ACE344@p brcas31.pbrc.edu> Message-ID: <1289951726l.909470l.0l@psu.edu> Thanks Tina. I will try that for the already frozen brains. Also I did some slicing today with some of my frozen brains and kept the block in the cryostat till it came to temperature and as compared to before I think I can say that the slices came out pretty well. I guess the temperature is the trick here. Well I am going to run some stains on them and see how they turn out and then I can say for sure that I have a good working protocol hopefully. These slices are for slide mounting not free floating. I do use the anti-roll plate and I did realize that it was a little off in its angle with respect to the knife and hence one another issue that needed fixing. Will let you on my progress. Thanks Liz for letting me know. Have you tried the Fluro Jade? The original, B or C? On Tue, Nov 16, 2010 05:33 PM, "Montina Van Meter" wrote: > Anjum, >The post-fix and sucrose are for the new harvests. > >Do you free-float the sections or mount them on slides? > >Make sure you allow the -80C block of tissue to sit in the cryostat for about >20 min. to allow the block to come up to the -20C temperature of the cryostat >chamber. There is no such thing as a "too hard block". Allowing it >to become the same temperature as the inside of the cryostat will solve that >problem. > >Warming up the block after freezing, than freezing it down again, is an >absolute no-no. This will also cause freeze/thaw artifact. > >Are you using an anti-roll plate when sectioning on the cryostat? That might >help flatten out the sections as they come off the knife edge. > >If you haven't already frozen the other brains I would place them in 20% >sucrose/PBS overnight. The next morning rinse off the sucrose with PBS for >about 30 min. Freeze the brain on dry ice in the OCT. If you have already >frozen all of the brains, you might take one of them and thaw it out. Place it >in the sucrose overnight and see if that helps with the cutting. It can't >hurt, since you are already having trouble getting sections. > >Good luck, >Tina > > > > > > >Montina J. Van Meter >Lab Manager >Autonomic Neuroscience >225-763-2564 >vanmetmj@pbrc.edu > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ANJUM PARKAR >Sent: Monday, November 15, 2010 9:24 AM >To: Histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Frozen brain slices > >Thanks Tina. I will keep in mind the thickness for staining purposes that is >once the slicing protocol gets optimized. So we use 200-300 ml of both PBS and >PFA for our perfusion. So the post fix and sucrose treatment you are >suggesting, are you suggesting for the new harvests alone which I will be doing >or the existing frozen perfused ones? >Anjum > >On Sun, Nov 14, 2010 10:35 PM, "Montina Van Meter" > >wrote: >> >Anhum, >>How much PBS and Paraformaldehyde do you put through the (rat or >>mouse). I flush a perfuse our rats with 150-200ml. of PBS and 500ml. >>of Para. Postfix for 2 hours and place in 20% sucrose overnight until >>the tisue sinks to the bottom of the vial. >>The morphology of your tissue is going to be compromised due to freeze >>artifact (lack of cryoprotectant) and there will be holes in your > >>sections. >> >>Actually, 30um thick sections are considered quite thick and are >>typically used for free floating techniques. Sections that are mounted >>on slides before IHC staining are much thinner (3-10um). I >usually >>cut my tissue between 30-40um and manually free float the sections for >>IHC or IF. >> >>Tina >> >> >>Sent from my iPhone >> >>On Nov 14, 2010, at 9:05 PM, "ANJUM PARKAR" > >>wrote: >> >> Thanks Tina. I will keep the sucrose suggestion in mind for future >> harvests. >> For now I need to figure out how to slice the already fixed frozen >> tissue. Will >> try to equilibrate in the cryostat itself as against room >> temperature and see >> how that goes. The knife angle is 13 degrees and recently sharpened >> and >> thickness of slices 30 microns which I believe is standard for >> sectioning. I >> have sectioned previously a lot so doing hands on is something I am >> familiar >> with. But I did realize every instrument is different and while >> doing a >> procedure it always needs to be optimized until it can become >> routine. Will let >> you know how things go. >> Anjum >> >> >> On Sun, Nov 14, 2010 02:21 PM, "Montina Van Meter" >>> > >> wrote: >> >> Anhum, >> 1. You MUST cryoprotect the fixed tissue in 15-30% sucrose (until it >> sinks) prior to cutting frozen sections. >> 2. Allow the -80C embedded tissue block to equilabrate to -20C in the >> cryostat for 15-20min. >> 3. Check the knife angle. Thickness? >> 4. Change knife or sharpen and make sure all screws are tight. >> 5. Freez/thawing of block is not a good idea (especially since it's >> not cryoprotected). You are introducing ice crystal artifact. >> 6. Check around your department (or Histology Core) to see if >>someone >> >> can instruct you on using the cryostat. It's always better to >> actually >> watch someone in addition to receiving written instructions. >> >> Good luck, >> Tina >> >> >> Sent from my iPhone >> >> On Nov 14, 2010, at 10:47 AM, "ANJUM PARKAR" >> >> wrote: >> >> Hello all, >> I have been having some histology issues in terms of rat brain >> slicing the past >> few months and hence looking for suggestions to fix them, having >> tried most of >> what I know from my past training and running of ideas real fast >> now. >> The following is the protocol I have used thus far:-1. Animal >> perfusion-a)Use >> PBS first, b) Use 4%PFA next and c) Harvest brain right after >> PFA. >> (Transcardial perfusion)2. Freeze brain by embedding in OCT >> compound >> using dry >> ice and then storing at -80 until ready to slice. Alternatively some >> times the >> -80 is used straight up with out dry ice freezing (still embedded in >> OCT).3. >> From standard protocol I do not do two things-a) No post fixation >> once brain is >> harvested and b) No sucrose. Reason being, I was taught that these >> two steps >> might result in large holes in tissue.4.For slicing, we have the >> older cryostat >> version (Real old version of CM3050S from Vibrotome-30 micron >> slices) which >> uses a fixed knife for slicing and has a chamber temp control and >> one for the >> specimen. The cryostat has not been serviced for a few years now.5. >> In attempt >> 1-I take the brain out of the -80 once the chamber temperature comes >> to -20 and >> mount the frozen mold on the chuck and attempt at slicing. Doing >> this I managed >> to get a few slices (that too with cuts and ripples) and after >> which >> the tissue >> just kept cracking and falling into pieces and hence could get no >> more >> slices.6. In attempt 2-I thought the brain may be too cold and too >> hard >> (perfusion and-80 freezing), so I defrosted the brain for 20 >> mins at >> room >> temperature before sticking into -20 cryostat. Between slices if I >> felt the >> tissue was too cold, I put my thumb on the specimen to warm it up. >> Still no >> luck.7. In attempt 3-I defrosted the brain completely to room >> temperature, took >> the brain out of the cryogel embedding (thinking that the mold was >> way too hard >> to cut through and hence cracking the tissue too) and attached it to >> chuck >> directly and tried slicing. Still no luck at all this time around. >> From what I have been reading online and from what I know, I >> primarily feel >> these are issues related with temperature of the brain and that it >> is too cold >> during cutting. I have tried different ways to bring the temperature >> down and I >> still have no success. I have eight other brains frozen using the >> protocol >> above and I really need to get slices out of them. Do you have any >> suggestions >> for me? Also do you think I should make any changes to the existing >> freezing >> protocol for the future brain harvesting? >> APPenn State University,PA >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > >-- >Anjum Parkar >Doctoral Candidate >Department of Bioengineering, >Penn State University, >206 Hallowell Building, >University Park, PA 16802. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anjum Parkar Doctoral Candidate Department of Bioengineering, Penn State University, 206 Hallowell Building, University Park, PA 16802. From MariAnn.Mailhiot <@t> leica-microsystems.com Tue Nov 16 18:15:54 2010 From: MariAnn.Mailhiot <@t> leica-microsystems.com (MariAnn.Mailhiot@leica-microsystems.com) Date: Tue Nov 16 18:16:30 2010 Subject: [Histonet] Disposable blade holder for a Leica 1512 rotary microtome. In-Reply-To: Message-ID: Bert (Embedded image moved to file: pic30695.jpg) 14036833012 : High profile blade rail (twin) - set 14036833013 : Low profile blade rail (twin) - set Hope this helps Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7841 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Clough, Bret" To Sent by: "histonet@lists.utsouthwestern.edu" histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Disposable blade holder 11/16/2010 11:09 for a Leica 1512 rotary microtome. AM I hope that someone on histonet could help me. Our research lab just acquired an old Leica 1512 in good working order but lacking a blade holder. Is it possible for us to find a blade holder for this machine or is this a futile cause? Any information you all could provide would be greatly appreciated. Thanks, Bret Clough Texas A&M Health Science Center Temple TX. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From histopathy <@t> hotmail.com Tue Nov 16 19:33:56 2010 From: histopathy <@t> hotmail.com (Pamela Gholston) Date: Tue Nov 16 19:34:01 2010 Subject: [Histonet] Current issues with Printmates and Checkmates Message-ID: Hello? Is anyone having issues with the Checkmate and/or Printmates? What is the opinion out there about using the "hot foil tape methodology?" Is the tape chemically resistant? Histopathy From Melissa.Kuhnla <@t> chsli.org Wed Nov 17 06:28:53 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed Nov 17 06:28:59 2010 Subject: [Histonet] Tissue loss and FISH Message-ID: Hello, We are a lab that has been running Pathvysion Her2 FISH for years without any major problems. Recently we have been experiencing more and more tissue section loss. I have noticed that some of the loss is starting during pretreatment. My temperatures for all steps are what they should be. During each temp. dependent step, we have two thermometers giving similar readings. Nothing has changes regarding our slide baking. I have a call into the vendor of the plus slides to verify the integrity of the lot. I have recently noticed the labeling on the pathvysion bottles is slightly different; I also have a call into them as well. Does anyone have any thoughts? Does anyone know of processing variables that may have lead to this???? Thank you, Melissa The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From micropathlabs <@t> yahoo.com Wed Nov 17 06:34:25 2010 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Wed Nov 17 06:34:30 2010 Subject: [Histonet] Tissue loss and FISH In-Reply-To: References: Message-ID: <561746.49280.qm@web57806.mail.re3.yahoo.com> We recently?had problems with our Superfrost Plus slides that we purchase from Cardinal.?We?had sections washing off and uneven staining.?We switched to VWR VistaVision Histobond Slides and have?had no further issues. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ? ________________________________ From: "Kuhnla, Melissa" To: histonet@lists.utsouthwestern.edu Sent: Wed, November 17, 2010 7:28:53 AM Subject: [Histonet] Tissue loss and FISH Hello, We are a lab that has been running Pathvysion Her2 FISH for years without any major problems.? Recently we have been experiencing more and more tissue section loss. I have noticed that some of the loss is starting during pretreatment. My temperatures for all steps are what they should be.? During each temp. dependent step, we have two thermometers giving similar readings. Nothing has changes regarding our slide baking.? I have a call into the vendor of the plus slides to verify the integrity of the lot.? I have recently noticed the labeling on the pathvysion bottles is slightly different; I also have a call into them as well.? Does anyone have any thoughts?? Does anyone know of processing variables that may have lead to this???? Thank you, Melissa The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail? and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> shorememorial.org Wed Nov 17 06:41:34 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Wed Nov 17 06:44:39 2010 Subject: [Histonet] Tissue loss and FISH In-Reply-To: <561746.49280.qm@web57806.mail.re3.yahoo.com> Message-ID: I have to agree with Sheila's statement. When we experience this during fish or immuno staining my first thing to check is temperature and the next thing is the slides. The slides almost always is the culprit. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Sheila Haas To Sent by: "Kuhnla, Melissa" histonet-bounces@ , lists.utsouthwest histonet@lists.utsouthwestern.edu ern.edu cc Subject 11/17/2010 07:34 Re: [Histonet] Tissue loss and FISH AM We recently?had problems with our Superfrost Plus slides that we purchase from Cardinal.?We?had sections washing off and uneven staining.?We switched to VWR VistaVision Histobond Slides and have?had no further issues. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ________________________________ From: "Kuhnla, Melissa" To: histonet@lists.utsouthwestern.edu Sent: Wed, November 17, 2010 7:28:53 AM Subject: [Histonet] Tissue loss and FISH Hello, We are a lab that has been running Pathvysion Her2 FISH for years without any major problems.? Recently we have been experiencing more and more tissue section loss. I have noticed that some of the loss is starting during pretreatment. My temperatures for all steps are what they should be.? During each temp. dependent step, we have two thermometers giving similar readings. Nothing has changes regarding our slide baking.? I have a call into the vendor of the plus slides to verify the integrity of the lot.? I have recently noticed the labeling on the pathvysion bottles is slightly different; I also have a call into them as well. Does anyone have any thoughts?? Does anyone know of processing variables that may have lead to this???? Thank you, Melissa The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail? and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Copyright 2007 Shore Memorial Hospital From histonet.nospam <@t> vneubert.com Wed Nov 17 08:28:03 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Nov 17 08:28:13 2010 Subject: [Histonet] Looking for picture of urinary bladder, murine In-Reply-To: References: Message-ID: <4CE3E673.6080409@vneubert.com> Hello Histoland, if there is anyone who can send me a digital picture of murine urinary bladder, H&E stained, Bouin-Hollande fixed if possible - please do so! Thank you, Valentin From laurie.colbert <@t> huntingtonhospital.com Wed Nov 17 09:10:19 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Nov 17 09:10:25 2010 Subject: [Histonet] Current issues with Printmates and Checkmates In-Reply-To: Message-ID: <57BE698966D5C54EAE8612E8941D768309FF6476@EXCHANGE3.huntingtonhospital.com> I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works fine for us - the print is chemically resistant. We use xylene for both processing and staining. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Gholston Sent: Tuesday, November 16, 2010 5:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current issues with Printmates and Checkmates Hello? Is anyone having issues with the Checkmate and/or Printmates? What is the opinion out there about using the "hot foil tape methodology?" Is the tape chemically resistant? Histopathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Wed Nov 17 10:04:40 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Wed Nov 17 10:04:43 2010 Subject: [Histonet] Current issues with Printmates and Checkmates In-Reply-To: <57BE698966D5C54EAE8612E8941D768309FF6476@EXCHANGE3.huntingtonhospital.com> Message-ID: <1298400300.254238.1290009880259.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> The ink also stays on through decal solutions which we needed.? We do 30 bone marrows a day any system that could not stand up to decal was a no go for us. Pam Marcum UAMS ----- Original Message ----- From: "Laurie Colbert" To: "Pamela Gholston" , histonet@lists.utsouthwestern.edu Sent: Wednesday, November 17, 2010 9:10:19 AM Subject: RE: [Histonet] Current issues with Printmates and Checkmates I have the Printmate and 5 Slidemates/PSLIMs. ?The hot foil tape works fine for us - the print is chemically resistant. ?We use xylene for both processing and staining. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Gholston Sent: Tuesday, November 16, 2010 5:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current issues with Printmates and Checkmates Hello? ?Is anyone having issues with the Checkmate and/or Printmates? ? What is the opinion out there about using the "hot foil tape methodology?" Is the tape chemically resistant? Histopathy ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Laurie Colbert" To: "Pamela Gholston" , histonet@lists.utsouthwestern.edu Sent: Wednesday, November 17, 2010 9:10:19 AM Subject: RE: [Histonet] Current issues with Printmates and Checkmates I have the Printmate and 5 Slidemates/PSLIMs. ?The hot foil tape works fine for us - the print is chemically resistant. ?We use xylene for both processing and staining. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Gholston Sent: Tuesday, November 16, 2010 5:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current issues with Printmates and Checkmates Hello? ?Is anyone having issues with the Checkmate and/or Printmates? ? What is the opinion out there about using the "hot foil tape methodology?" Is the tape chemically resistant? Histopathy ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpjones <@t> srhs-pa.org Wed Nov 17 10:05:13 2010 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Wed Nov 17 10:05:21 2010 Subject: [Histonet] 10% Neutral Buffered Formalin - Methanol? Message-ID: <4AE8039AEA096143B965CBC6D092166802351B754B@EXCH2007.srhs-pa.org> Greetings to all of you in Histoland. Our lab recently switched from using a formalin substitute to using 10% Neutral Buffered Formalin. Our Pathologists have been unhappy with the small tissues, like GI biopsies and prostate cores. They say they are seeing too much chatter and poor nuclear detail. We have adjusted our processing times with only mildly better results. Today, I arrived at work to find staff cramming boxes and boxes of prefilled formalin vials into flame cabinets, as JCAHO is here this week. It occurred to me that 10% NBF was not considered flammable when we used it years ago, and I was surprised to find that the MSDS for the bottles we had ordered listed methanol as an ingredient. I immediately went back to my early days in Histo, when we made up 10% NBF ourselves from 37% concentrate; and we did not have any alcohol in our "recipe". I thought I had discovered our whole problem! However, upon further research, we have found that most prefilled bottles DO contain methanol. The large 20 litre cube, however does not list methanol as an ingredient. So, my questions are many. Does that inclusion of methanol contribute to the drying out of tissues that we are seeing? Does anyone sell prefilled bottles that contain methanol-free formalin? And, finally, does anyone have any other thoughts or suggestions? I should add that we use Toluene as our clearing agent, because our former Pathologist believed it was less harsh on the tissues; and we are running our tissues on the Thermo Excelsior. We are running small biopsies and large pieces of tissue together, which I know is not optimal, but we are a small hospital and one processor is it! I am not a chemist and would appreciate any advice. Thanks in advance. ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From gu.lang <@t> gmx.at Wed Nov 17 10:18:12 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Nov 17 10:18:21 2010 Subject: AW: [Histonet] 10% Neutral Buffered Formalin - Methanol? In-Reply-To: <4AE8039AEA096143B965CBC6D092166802351B754B@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D092166802351B754B@EXCH2007.srhs-pa.org> Message-ID: <844FAC22360A4EBEA236BD857BCF9B9F@dielangs.at> I guess the producer of the NBF has to state the Methanol-content, because 37% Formaldehyd (stocksolution) usually is made stable with an amount of methanol. That does not influence the fixation performance. There's a study by Fox about this. And he saw shrinkage of tissue only when using the concentrated 37% Formaldehyd pure on tissue. This effect he thought was caused by the Methanol content. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jones, Laura Gesendet: Mittwoch, 17. November 2010 17:05 An: Histonet (E-mail) Betreff: [Histonet] 10% Neutral Buffered Formalin - Methanol? Greetings to all of you in Histoland. Our lab recently switched from using a formalin substitute to using 10% Neutral Buffered Formalin. Our Pathologists have been unhappy with the small tissues, like GI biopsies and prostate cores. They say they are seeing too much chatter and poor nuclear detail. We have adjusted our processing times with only mildly better results. Today, I arrived at work to find staff cramming boxes and boxes of prefilled formalin vials into flame cabinets, as JCAHO is here this week. It occurred to me that 10% NBF was not considered flammable when we used it years ago, and I was surprised to find that the MSDS for the bottles we had ordered listed methanol as an ingredient. I immediately went back to my early days in Histo, when we made up 10% NBF ourselves from 37% concentrate; and we did not have any alcohol in our "recipe". I thought I had discovered our whole problem! However, upon further research, we have found that most prefilled bottles DO contain methanol. The large 20 litre cube, however does not list methanol as an ingredient. So, my questions are many. Does that inclusion of methanol contribute to the drying out of tissues that we are seeing? Does anyone sell prefilled bottles that contain methanol-free formalin? And, finally, does anyone have any other thoughts or suggestions? I should add that we use Toluene as our clearing agent, because our former Pathologist believed it was less harsh on the tissues; and we are running our tissues on the Thermo Excelsior. We are running small biopsies and large pieces of tissue together, which I know is not optimal, but we are a small hospital and one processor is it! I am not a chemist and would appreciate any advice. Thanks in advance. ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dbeil1 <@t> hotmail.com Wed Nov 17 10:25:15 2010 From: dbeil1 <@t> hotmail.com (Damaris Beil) Date: Wed Nov 17 10:25:19 2010 Subject: [Histonet] Patients' Pathology Requisitions Message-ID: Hello everyone, Could someone please tell how long we are required to keep the patients' pathology requisitions in the lab, after the case has been read or signed out. Thank You! From LSebree <@t> uwhealth.org Wed Nov 17 10:26:11 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Nov 17 10:26:15 2010 Subject: [Histonet] Patients' Pathology Requisitions In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E273839A0C8@UWHC-MAIL01.uwhis.hosp.wisc.edu> We keep our 2 years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Damaris Beil Sent: Wednesday, November 17, 2010 10:25 AM To: histonet Subject: [Histonet] Patients' Pathology Requisitions Hello everyone, Could someone please tell how long we are required to keep the patients' pathology requisitions in the lab, after the case has been read or signed out. Thank You! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbrya <@t> lexclin.com Wed Nov 17 10:37:24 2010 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Wed Nov 17 10:37:31 2010 Subject: [Histonet] room temp monitoring Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BA2D678C@EXCHANGESB> ANP. 21390 Reagent Storage All reagents are stored as recommended by the manufacturer. The evidence of compliance now states you must show records such as refrigerator, freezer and room temperature monitoring as applicable. We keep records of the refrigerator temps used to store our IHC but we have reagents used for cytology and histology in various cabinets in our laboratory. Someone suggested this new reg. means we must use thermometers to keep logs of the room temp in each cabinet where reagents are stored. We have about 6 cabinets with room temp. reagents. How do you interpret this reference to room temperature monitoring? Thank you in advance for your response. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From mcauliff <@t> umdnj.edu Wed Nov 17 10:42:39 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Nov 17 10:39:51 2010 Subject: [Histonet] 10% Neutral Buffered Formalin - Methanol? In-Reply-To: <4AE8039AEA096143B965CBC6D092166802351B754B@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D092166802351B754B@EXCH2007.srhs-pa.org> Message-ID: <4CE405FF.6030505@umdnj.edu> Commercially purchased 37% formaldehyde has had a small amount (about 1.5% I think) of methanol added to it for many, many years. It helps to prevent the polymerization of formaldehyde into insoluble paraformaldehyde. It certainly does not make the stock solution flammable and it is not contributing to drying out of your tissues. Those who want methanol-free formalin make it from paraformaldehyde but for LM there is no point. Geoff Jones, Laura wrote: > Greetings to all of you in Histoland. Our lab recently switched from using a formalin substitute to using 10% Neutral Buffered Formalin. Our Pathologists have been unhappy with the small tissues, like GI biopsies and prostate cores. They say they are seeing too much chatter and poor nuclear detail. We have adjusted our processing times with only mildly better results. > > Today, I arrived at work to find staff cramming boxes and boxes of prefilled formalin vials into flame cabinets, as JCAHO is here this week. It occurred to me that 10% NBF was not considered flammable when we used it years ago, and I was surprised to find that the MSDS for the bottles we had ordered listed methanol as an ingredient. I immediately went back to my early days in Histo, when we made up 10% NBF ourselves from 37% concentrate; and we did not have any alcohol in our "recipe". I thought I had discovered our whole problem! However, upon further research, we have found that most prefilled bottles DO contain methanol. The large 20 litre cube, however does not list methanol as an ingredient. > > So, my questions are many. Does that inclusion of methanol contribute to the drying out of tissues that we are seeing? Does anyone sell prefilled bottles that contain methanol-free formalin? And, finally, does anyone have any other thoughts or suggestions? I should add that we use Toluene as our clearing agent, because our former Pathologist believed it was less harsh on the tissues; and we are running our tissues on the Thermo Excelsior. We are running small biopsies and large pieces of tissue together, which I know is not optimal, but we are a small hospital and one processor is it! I am not a chemist and would appreciate any advice. Thanks in advance. > > > > ________________________________ > > Sharon Regional Health System is the area's largest hospital > and provider of health care services. Visit us online at > http://www.sharonregional.com for a complete listing of our > services, primary care physicians and specialists, and satellite locations. > > Confidentiality Note: This message is intended for use > only by the individual or entity to which it is addressed > and may contain information that is privileged, > confidential, and exempt from disclosure under applicable > law. If the reader of this message is not the intended > recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution or > copying of this communication is strictly prohibited. If > you have received this communication in error, please > contact the sender immediately and destroy the material in > its entirety, whether electronic or hard copy. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mucram11 <@t> comcast.net Wed Nov 17 10:46:52 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Wed Nov 17 10:46:57 2010 Subject: [Histonet] Histologists and Supervisor in Little Rock AR Message-ID: <119387764.256496.1290012412694.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Good Morning, We are looking for a Working Histology Supervisor at UAMS in Little rock AR. We are also looking for two histologists one for routine surgical pathology and one for the IHC area.? There will be rotation in the future between Histology and IHC. If you are interested in these positions please contact me by e-mail and we can discuss details. Pam Marcum AP Manager UAMS From mucram11 <@t> comcast.net Wed Nov 17 10:48:04 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Wed Nov 17 10:48:06 2010 Subject: [Histonet] Histologists and Supervior Needed Little Rock AR In-Reply-To: <8C023B4AB999614BA4791BAEB26E273839A0C8@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <2140409876.256542.1290012484443.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Good Morning, We are looking for a Working Histology Supervisor at UAMS in Little rock AR. We are also looking for two histologists one for routine surgical pathology and one for the IHC area.? There will be rotation in the future between Histology and IHC. If you are interested in these positions please contact me by e-mail and we can discuss details. Pam Marcum AP Manager UAMS ----- Original Message ----- From: "Sebree Linda A" To: "Damaris Beil" , "histonet" Sent: Wednesday, November 17, 2010 10:26:11 AM Subject: RE: [Histonet] Patients' Pathology Requisitions We keep our 2 years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Damaris Beil Sent: Wednesday, November 17, 2010 10:25 AM To: histonet Subject: [Histonet] Patients' Pathology Requisitions Hello everyone, ? Could someone please tell how long we are required to keep the patients' pathology requisitions in the lab, after the case has been read or signed out. ? Thank You! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHargrove <@t> urhcs.org Wed Nov 17 11:08:59 2010 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Wed Nov 17 11:09:06 2010 Subject: [Histonet] ER/PR reporting with disclaimer on Ventana Ultra vs.XT Message-ID: We are replacing out Ventana XT with an Ultra. We are currently running our ER's and Pr's on our Ventana XT. The tech doing the Immuno's told me she cannot do them on the Ultra because it has not been approved yet. We currently are not using a disclaimer on the Er/Pr reports because these have been validated on the Ultra but not the XT. The Path's only report positive vs.negative but I do not understand the "disclaimer" use I see on other reports. She is either not communicating well with me or is confused. Either way she has me confused. Can anyone help? And does anyone need an extra machine? We don't have room for 2. From LSebree <@t> uwhealth.org Wed Nov 17 11:19:28 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Nov 17 11:19:32 2010 Subject: [Histonet] room temp monitoring In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BA2D678C@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BA2D678C@EXCHANGESB> Message-ID: <8C023B4AB999614BA4791BAEB26E273839A0C9@UWHC-MAIL01.uwhis.hosp.wisc.edu> Wouldn't all your cabinets be the same temperature as that of the room the cabinets are in? We just monitor the general ambient temperature because some of our reagents state that storage is at room temperature. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Wednesday, November 17, 2010 10:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] room temp monitoring ANP. 21390 Reagent Storage All reagents are stored as recommended by the manufacturer. The evidence of compliance now states you must show records such as refrigerator, freezer and room temperature monitoring as applicable. We keep records of the refrigerator temps used to store our IHC but we have reagents used for cytology and histology in various cabinets in our laboratory. Someone suggested this new reg. means we must use thermometers to keep logs of the room temp in each cabinet where reagents are stored. We have about 6 cabinets with room temp. reagents. How do you interpret this reference to room temperature monitoring? Thank you in advance for your response. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Nov 17 11:29:52 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Nov 17 11:29:56 2010 Subject: [Histonet] 10% Neutral Buffered Formalin - Methanol? In-Reply-To: <4CE405FF.6030505@umdnj.edu> References: <4AE8039AEA096143B965CBC6D092166802351B754B@EXCH2007.srhs-pa.org> <4CE405FF.6030505@umdnj.edu> Message-ID: A related question: I was making Serra's fix (which has no water in it) and I tried to use buffered formalin. This turned the solution white (which happens when Serra's has water in it). Another brand of formalin (which did not say it was buffered) worked fine. What's in the buffering that has water in it? Is the methanol diluted with water in buffered formalin? The recipe for Serra's is 6ml 100% EtOH 3ml 37% formaldehyde (aka formalin) 1ml glacial acetic acid Perhaps the buffer in your buffered formalin is causing the problem--maybe you need unbuffered formalin. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Wed, Nov 17, 2010 at 11:42 AM, Geoff McAuliffe wrote: > Commercially purchased 37% formaldehyde has had a small amount (about 1.5% I > think) of methanol added to it for many, many years. It helps to prevent the > polymerization of formaldehyde into insoluble paraformaldehyde. It certainly > does not make the stock solution flammable and it is not contributing to > drying out of your tissues. Those who want methanol-free formalin make it > from paraformaldehyde but for LM there is no point. > > Geoff > > > Jones, Laura wrote: >> >> Greetings to all of you in Histoland. ?Our lab recently switched from >> using a formalin substitute to using 10% Neutral Buffered Formalin. ?Our >> Pathologists have been unhappy with the small tissues, like GI biopsies and >> prostate cores. ?They say they are seeing too much chatter and poor nuclear >> detail. ?We have adjusted our processing times with only mildly better >> results. >> >> Today, I arrived at work to find staff cramming boxes and boxes of >> prefilled formalin vials into flame cabinets, as JCAHO is here this week. >> ?It occurred to me that 10% NBF was not considered flammable when we used it >> years ago, and I was surprised to find that the MSDS for the bottles we had >> ordered listed methanol as an ingredient. ?I immediately went back to my >> early days in Histo, when we made up 10% NBF ourselves from 37% concentrate; >> and we did not have any alcohol in our "recipe". ?I thought I had discovered >> our whole problem! ?However, upon further research, we have found that most >> prefilled bottles DO contain methanol. ?The large 20 litre cube, however >> does not list methanol as an ingredient. >> >> So, my questions are many. ?Does that inclusion of methanol contribute to >> the drying out of tissues that we are seeing? ?Does anyone sell prefilled >> bottles that contain methanol-free formalin? ?And, finally, does anyone have >> any other thoughts or suggestions? ?I should add that we use Toluene as our >> clearing agent, because our former Pathologist believed it was less harsh on >> the tissues; and we are running our tissues on the Thermo Excelsior. ?We are >> running small biopsies and large pieces of tissue together, which I know is >> not optimal, but we are a small hospital and one processor is it! ?I am not >> a chemist and would appreciate any advice. ?Thanks in advance. >> >> >> >> ?________________________________ >> >> Sharon Regional Health System is the area's largest hospital >> and provider of health care services. Visit us online at >> http://www.sharonregional.com for a complete listing of our >> services, primary care physicians and specialists, and satellite >> locations. >> >> Confidentiality Note: This message is intended for use >> only by the individual or entity to which it is addressed >> and may contain information that is privileged, >> confidential, and exempt from disclosure under applicable >> law. If the reader of this message is not the intended >> recipient or the employee or agent responsible for >> delivering the message to the intended recipient, you are >> hereby notified that any dissemination, distribution or >> copying of this communication is strictly prohibited. If >> you have received this communication in error, please >> contact the sender immediately and destroy the material in >> its entirety, whether electronic or hard copy. Thank you. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Luis.Chiriboga <@t> nyumc.org Wed Nov 17 11:29:30 2010 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Wed Nov 17 11:30:03 2010 Subject: [Histonet] Oil Red O Message-ID: Hi Everyone Posting this for a colleague who is doing oil red o staining on carotid plaques and is seeing both large globular and abundant fine small globular staining. Localization is correct but she is concerned about the amount and size variability of the staining pattern. She has been using the propylene glycol method on frozen tissue and has tried a few variations but keeps getting the same pattern. Can provide a picture if you email me offline . Any advice would be helpful. Thanks Luis ------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
================================= 
From mcauliff <@t> umdnj.edu  Wed Nov 17 11:45:25 2010
From: mcauliff <@t> umdnj.edu (Geoff McAuliffe)
Date: Wed Nov 17 11:42:31 2010
Subject: [Histonet] 10% Neutral Buffered Formalin - Methanol?
In-Reply-To: 
References: <4AE8039AEA096143B965CBC6D092166802351B754B@EXCH2007.srhs-pa.org>
	<4CE405FF.6030505@umdnj.edu>
	
Message-ID: <4CE414B5.8090006@umdnj.edu>

Serra's fixative does have water in it, the water is from the 
formaldehyde. 37% formaldehyde is water saturated with formaldehyde gas 
to about 37%.
The precipitate is probably from the buffering compound, phosphate perhaps.

Geoff

Emily Sours wrote:
> A related question:
> I was making Serra's fix (which has no water in it) and I tried to use
> buffered formalin.  This turned the solution white (which happens when
> Serra's has water in it).  Another brand of formalin (which did not
> say it was buffered) worked fine.
> What's in the buffering that has water in it? Is the methanol diluted
> with water in buffered formalin?
> The recipe for Serra's is
> 6ml 100% EtOH
> 3ml 37% formaldehyde (aka formalin)
> 1ml glacial acetic acid
>
> Perhaps the buffer in your buffered formalin is causing the
> problem--maybe you need unbuffered formalin.
>
> Emily
> --
> Outside of a dog, a book is man's best friend. Inside of a dog it's
> too dark to read.
> --Groucho Marx
>
>
>
> On Wed, Nov 17, 2010 at 11:42 AM, Geoff McAuliffe  wrote:
>   
>> Commercially purchased 37% formaldehyde has had a small amount (about 1.5% I
>> think) of methanol added to it for many, many years. It helps to prevent the
>> polymerization of formaldehyde into insoluble paraformaldehyde. It certainly
>> does not make the stock solution flammable and it is not contributing to
>> drying out of your tissues. Those who want methanol-free formalin make it
>> from paraformaldehyde but for LM there is no point.
>>
>> Geoff
>>
>>
>> Jones, Laura wrote:
>>     
>>> Greetings to all of you in Histoland.  Our lab recently switched from
>>> using a formalin substitute to using 10% Neutral Buffered Formalin.  Our
>>> Pathologists have been unhappy with the small tissues, like GI biopsies and
>>> prostate cores.  They say they are seeing too much chatter and poor nuclear
>>> detail.  We have adjusted our processing times with only mildly better
>>> results.
>>>
>>> Today, I arrived at work to find staff cramming boxes and boxes of
>>> prefilled formalin vials into flame cabinets, as JCAHO is here this week.
>>>  It occurred to me that 10% NBF was not considered flammable when we used it
>>> years ago, and I was surprised to find that the MSDS for the bottles we had
>>> ordered listed methanol as an ingredient.  I immediately went back to my
>>> early days in Histo, when we made up 10% NBF ourselves from 37% concentrate;
>>> and we did not have any alcohol in our "recipe".  I thought I had discovered
>>> our whole problem!  However, upon further research, we have found that most
>>> prefilled bottles DO contain methanol.  The large 20 litre cube, however
>>> does not list methanol as an ingredient.
>>>
>>> So, my questions are many.  Does that inclusion of methanol contribute to
>>> the drying out of tissues that we are seeing?  Does anyone sell prefilled
>>> bottles that contain methanol-free formalin?  And, finally, does anyone have
>>> any other thoughts or suggestions?  I should add that we use Toluene as our
>>> clearing agent, because our former Pathologist believed it was less harsh on
>>> the tissues; and we are running our tissues on the Thermo Excelsior.  We are
>>> running small biopsies and large pieces of tissue together, which I know is
>>> not optimal, but we are a small hospital and one processor is it!  I am not
>>> a chemist and would appreciate any advice.  Thanks in advance.
>>>
>>>
>>>
>>>  ________________________________
>>>
>>> Sharon Regional Health System is the area's largest hospital
>>> and provider of health care services. Visit us online at
>>> http://www.sharonregional.com for a complete listing of our
>>> services, primary care physicians and specialists, and satellite
>>> locations.
>>>
>>> Confidentiality Note: This message is intended for use
>>> only by the individual or entity to which it is addressed
>>> and may contain information that is privileged,
>>> confidential, and exempt from disclosure under applicable
>>> law. If the reader of this message is not the intended
>>> recipient or the employee or agent responsible for
>>> delivering the message to the intended recipient, you are
>>> hereby notified that any dissemination, distribution or
>>> copying of this communication is strictly prohibited. If
>>> you have received this communication in error, please
>>> contact the sender immediately and destroy the material in
>>> its entirety, whether electronic or hard copy. Thank you.
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet@lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>>
>>>
>>>       
>> --
>> --
>> **********************************************
>> Geoff McAuliffe, Ph.D.
>> Neuroscience and Cell Biology
>> Robert Wood Johnson Medical School
>> 675 Hoes Lane, Piscataway, NJ 08854
>> voice: (732)-235-4583 mcauliff@umdnj.edu
>> **********************************************
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>     
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************



From pkarlisch <@t> hmc.psu.edu  Wed Nov 17 12:17:46 2010
From: pkarlisch <@t> hmc.psu.edu (Patricia Karlisch)
Date: Wed Nov 17 12:18:23 2010
Subject: [Histonet] RE: Looking for an EM technologist
Message-ID: <4CE3D5FA.07B7.008C.1@hmc.psu.edu>

All,
  Hershey Medical Center in Hershey PA, is looking for a
Histotechnologist with EM experience for their EM lab.  Candidate may
have EM experience in research but must have at least an Associates
Degree in one of the sciences and some histology background.  If anyone
is interested, please contact me and I can give anyone the information
for applying on line.
Patricia Karlisch, Supervisor, Histology
Pathology and Laboratory Medicine
 
Pat Karlisch
Supervisor, Histology, Pathology and Laboratory Medicine
Penn State Milton S. Hershey Medical Center
Mail Code H179
Hershey, PA 17033
Phone (717) 531-6072
Fax: (717) 531- 7741
email: pkarlisch@psu.edu 
 
*****E-Mail Confidentiality Notice*****
This message (including any attachments) contains information intended
for a specific individual(s) and purpose that may be privileged,
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applicable law.  Any inappropriate use, distribution or copying of the
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penalty.  If you have received this transmission in error, please reply
to the sender indicating this error and delete the transmission from
your system immediately.
From billodonnell <@t> catholichealth.net  Wed Nov 17 13:16:26 2010
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Wed Nov 17 13:16:43 2010
Subject: [Histonet] room temp monitoring
In-Reply-To: <8C023B4AB999614BA4791BAEB26E273839A0C9@UWHC-MAIL01.uwhis.hosp.wisc.edu>
References: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BA2D678C@EXCHANGESB>
	<8C023B4AB999614BA4791BAEB26E273839A0C9@UWHC-MAIL01.uwhis.hosp.wisc.edu>
Message-ID: 

Temps MIGHT be different. Do they have doors? What's behind the wall
(poorly insulated, hot water pipes) All of that being said, the point
might be are they within a range? Most likely they are, especially since
the lab establishes the range. 5 degrees Celcius on either side of 25
should keep everything in nice order. I do not monitor cabinets, but
ambient room temp because my range is wide enough to handle a reasonable
flux in temp.

Hope this helps more than confuses - Bill

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree
Linda A
Sent: Wednesday, November 17, 2010 11:19 AM
To: Carol Bryant; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] room temp monitoring 

Wouldn't all your cabinets be the same temperature as that of the room
the cabinets are in?  We just monitor the general ambient temperature
because some of our reagents state that storage is at room temperature. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Wednesday, November 17, 2010 10:37 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] room temp monitoring 

ANP. 21390 Reagent Storage
All reagents are stored as recommended by the manufacturer.
The evidence of compliance now states you must show records such as
refrigerator, freezer and room temperature monitoring as applicable.

We keep records of the refrigerator temps used to store our IHC but we
have reagents used for cytology and histology in various cabinets in our
laboratory.  Someone suggested this new reg. means we must use
thermometers to keep logs of the room temp in each cabinet where
reagents are stored.  We have about 6 cabinets with room temp. reagents.

How do you interpret this reference to room temperature monitoring?

Thank you in advance for your response.

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya@lexclin.com



NOTICE OF CONFIDENTIALITY

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From darkdaym <@t> comcast.net  Wed Nov 17 13:53:32 2010
From: darkdaym <@t> comcast.net (Mark Ray)
Date: Wed Nov 17 13:53:39 2010
Subject: [Histonet] 10% Neutral Buffered Formalin - Methanol?
In-Reply-To: <4CE405FF.6030505@umdnj.edu>
References: <4AE8039AEA096143B965CBC6D092166802351B754B@EXCH2007.srhs-pa.org>
	<4CE405FF.6030505@umdnj.edu>
Message-ID: <4CE432BC.3060406@comcast.net>

The Methanol concentration of Formalin, 37% is frequently described on 
the MSDS as 10-15%.  However, I believe the actual Methanol 
concentration is  normally at the low end of that range.  Both 
Formaldehyde  Gas and Methanol are Flammable.

Commercial 10% NBF typically  contains about 1.1% Methanol and 3.7% 
Formaldehyde.  The  Flash Point of this water solution defines it as a 
Combustible Liquid.  It is not correct to call  it or handle as as a 
Flammable Liquid and it does not normally require Flammable Liquid 
storage and precautions.  Let the Safety Officers of your institution 
determine how you should handle and store these quantities of a 
Combustible Liquid.

Regards,
Mark



On 11/17/2010 10:42 AM, Geoff McAuliffe wrote:
> Commercially purchased 37% formaldehyde has had a small amount (about 
> 1.5% I think) of methanol added to it for many, many years. It helps 
> to prevent the polymerization of formaldehyde into insoluble 
> paraformaldehyde. It certainly does not make the stock solution 
> flammable and it is not contributing to drying out of your tissues. 
> Those who want methanol-free formalin make it from paraformaldehyde 
> but for LM there is no point.
>
> Geoff
>
>
> Jones, Laura wrote:
>> Greetings to all of you in Histoland.  Our lab recently switched from 
>> using a formalin substitute to using 10% Neutral Buffered Formalin.  
>> Our Pathologists have been unhappy with the small tissues, like GI 
>> biopsies and prostate cores.  They say they are seeing too much 
>> chatter and poor nuclear detail.  We have adjusted our processing 
>> times with only mildly better results.
>>
>> Today, I arrived at work to find staff cramming boxes and boxes of 
>> prefilled formalin vials into flame cabinets, as JCAHO is here this 
>> week.  It occurred to me that 10% NBF was not considered flammable 
>> when we used it years ago, and I was surprised to find that the MSDS 
>> for the bottles we had ordered listed methanol as an ingredient.  I 
>> immediately went back to my early days in Histo, when we made up 10% 
>> NBF ourselves from 37% concentrate; and we did not have any alcohol 
>> in our "recipe".  I thought I had discovered our whole problem!  
>> However, upon further research, we have found that most prefilled 
>> bottles DO contain methanol.  The large 20 litre cube, however does 
>> not list methanol as an ingredient.
>>
>> So, my questions are many.  Does that inclusion of methanol 
>> contribute to the drying out of tissues that we are seeing?  Does 
>> anyone sell prefilled bottles that contain methanol-free formalin?  
>> And, finally, does anyone have any other thoughts or suggestions?  I 
>> should add that we use Toluene as our clearing agent, because our 
>> former Pathologist believed it was less harsh on the tissues; and we 
>> are running our tissues on the Thermo Excelsior.  We are running 
>> small biopsies and large pieces of tissue together, which I know is 
>> not optimal, but we are a small hospital and one processor is it!  I 
>> am not a chemist and would appreciate any advice.  Thanks in advance.
>>
>>
>>
>>   ________________________________
>>
>> Sharon Regional Health System is the area's largest hospital
>> and provider of health care services. Visit us online at
>> http://www.sharonregional.com for a complete listing of our
>> services, primary care physicians and specialists, and satellite 
>> locations.
>>
>> Confidentiality Note: This message is intended for use
>> only by the individual or entity to which it is addressed
>> and may contain information that is privileged,
>> confidential, and exempt from disclosure under applicable
>> law. If the reader of this message is not the intended
>> recipient or the employee or agent responsible for
>> delivering the message to the intended recipient, you are
>> hereby notified that any dissemination, distribution or
>> copying of this communication is strictly prohibited. If
>> you have received this communication in error, please
>> contact the sender immediately and destroy the material in
>> its entirety, whether electronic or hard copy. Thank you.
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>
>


From histologydirector <@t> gmail.com  Wed Nov 17 14:12:37 2010
From: histologydirector <@t> gmail.com (Histology Director)
Date: Wed Nov 17 14:12:42 2010
Subject: [Histonet] GU pathology laboratory looking for histotechs/
 cytotechs in Columbus, Ohio
Message-ID: 

Looking for experienced histology technicians and cytotechnologists in
Columbus, Ohio for new GU pathology laboratory. Excellent compensation with
great benefits. Experience with IHC, FISH, and GU pathology a plus. Send
resume or CV to careers@aksm.com.
From kc <@t> ka-recruiting.com  Wed Nov 17 14:37:27 2010
From: kc <@t> ka-recruiting.com (K.C. Carpenter)
Date: Wed Nov 17 14:37:31 2010
Subject: [Histonet] Histology Jobs
Message-ID: <1845011159.1290026247494.JavaMail.cfservice@sl4app3>






Dear Histonet Community,

 

I hope all is well with you since we were last in touch.  As a reminder I am a one of the founders of a healthcare recruiting firm.  I help Lab Professionals find permanent employment and I wanted to see if you are interested in exploring other career opportunities?  We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Histology Supervisor and Histotechnologist job with a Lab located in Southern New Hampshire. 





 







My client is a Pre-IPO Pathology company who is quickly establishing itself as an industry leader. They are looking to fill 2 positions in their state of the art lab located in Southern NH.  They have a bench Histology Position and a Histology Supervisor job open due to a surge in volume and long term growth plans. Both positions require a HT (ASCP) certification with a minimum of 2 years of bench experience. To be considered for the supervisor role you'll also need prior lead or supervisory experience.   My clients offers one of the best compensation & benefits package around including relocation assistance when necessary.   If you are interested in learning more about this position, please call or email me at kc@ka-recruiting.com

 







Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates.

 



Current Histology Openings: 

NH - Histology Supervisor

NH - Histotechnologist

NY - Upstate - Histotechnologist

NY - Long Island - Histology Lab Manager

NY - New York City - Histotechnologist - 3rd shift


MI - Histology Manager 


OK - Histology Supervisor

GA - Histology Supervisor

NV - Las Vegas - Histotechnologist - 3rd shift

TN - Histotechnologist

TX - Histology Supervisor



 






















If you're interested in any of these opportunities or are currently looking for employment then please reply with an updated resume and let me know when you're available to talk.  If this is not the right time for you to make a career change then please let me know if you can refer someone who is.  We offer a generous referral bonus for anyone you refer to us that we place into any position across the country.  

 


To learn more about job openings in other parts of the country please contact me or visit our website at www.ka-recruiting.com.  


















 

Sincerely,























KC Carpenter



K.A. Recruiting, Inc.


10 Post Office Square, 8th Floor South


Boston, MA 02109


P: (617) 692-2949


F: (617) 507-8009



kc@ka-recruiting.com



www.ka-recruiting.com
  









From jstaruk <@t> masshistology.com  Wed Nov 17 14:53:53 2010
From: jstaruk <@t> masshistology.com (jstaruk)
Date: Wed Nov 17 14:53:54 2010
Subject: [Histonet] Histology position in Massachusetts
In-Reply-To: <4CE3D5FA.07B7.008C.1@hmc.psu.edu>
Message-ID: 

HISTOLOGIST - Mass Histology Service, Inc. is a busy, well established,
rapidly growing, highly reputable private, GLP-compliant histopathology lab
located in central MA.  We are looking for an experienced histologist with a
strong background in routine and special stains, frozen sections,
immunohistochemistry and general histology procedures.  Must be HT certified
or eligible.  Salary is based on experience and quality of work.  We offer
great benefits and a pleasant work environment.  This is NOT a production
line "slide factory".  Please visit our homepages for a tour of our new
facilities.  E-mail your cover letter, resume and salary requirements to
info@masshistology.com.

Thanks

Jim

_______________________
James E. Staruk HT(ASCP)
 www.masshistology.com
   www.nehorselabs.com


From relia1 <@t> earthlink.net  Wed Nov 17 14:55:55 2010
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Wed Nov 17 14:55:56 2010
Subject: [Histonet] RELIA Histology Job Alert 11/17/10
Message-ID: 

Hi Histonetters!
I hope everyone is having a great day.  I wanted to take just a minute
of your time to tell you about some histology opportunities that I am
working on.  All of my positions are full time permanent jobs.  My
clients offer excellent compensation, benefits, relocation assistance
and great places to work.
I know that as the holidays draw closer a job change is one of the last
things on most people's minds however my clients are willing to move
right away or work with you after the holidays whichever you prefer.
 
Here is a list of my current openings:
MANAGEMENT
LA - Histology Supervisor
AR ? Director of Core Lab
CA ? Histology Lab Manager
NY-Quality Assurance Manager
 
HISTOTECHNICIANS/HISTOTECHNOLOGISTS
MD- Near Hagerstown day shift state of the art lab
TN ? Nashville Histotech 2 night shift positions avail.
TN - Chattanooga Night Shift Grossing histotech
FL- Central Florida Histology Product Sales
IN ? South of Chicago in Northern IN Histology Tech
GA ? Southern Coastal area ? Histotech with IHC ASCP HTL required.
NY-    Suffern NYS license/Elig day and night shift 
MA ? Boston Area several shifts available dermpath and general
FL ? Orlando State of FLTechnologist license and 2 yrs exper req.
CA - Tech Support/Inside Sales strong background in TEM
 
I realize that there are a lot of recruiters out there contacting you
about opportunities and I want to remind you that I am the only
recruiter who offers you over 25 years of experience helping people find
the right position at the right time and 9 years exclusively in
histology.  I offer a practice that works exclusively in the nationwide
permanent placement of histology professionals.  Your job search will be
confidential and tailored specifically to your needs.
 
If you or anyone you know might be interested in a new opportunity
please contact me toll free at 866-607-3542 or relia1@earthlink.net  
 
Thanks-Pam
 
Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From jaylundgren <@t> gmail.com  Wed Nov 17 16:40:35 2010
From: jaylundgren <@t> gmail.com (Jay Lundgren)
Date: Wed Nov 17 16:40:40 2010
Subject: [Histonet] Partially muddy slides
In-Reply-To: 
References: 
	
Message-ID: 

     Get rid of all your recycled reagents.  Try running new reagents all
the way though.  Also, increase your rinse times and agitation in the
rinse.  I'll bet this will solve your problem.

                                                       Jay A. Lundgren M.S.,
HTL (ASCP)
From jaylundgren <@t> gmail.com  Wed Nov 17 16:45:47 2010
From: jaylundgren <@t> gmail.com (Jay Lundgren)
Date: Wed Nov 17 16:45:51 2010
Subject: [Histonet] Oil Red O
In-Reply-To: 
References: 
Message-ID: 

     That's the way it normally looks, kinda globby. ORO is really not that
soluble in fat, just more soluble than the prop. glycol.

                                                Jay A. Lundgren M.S., HTL
(ASCP)
From jnocito <@t> satx.rr.com  Wed Nov 17 17:54:26 2010
From: jnocito <@t> satx.rr.com (Joe Nocito)
Date: Wed Nov 17 17:54:40 2010
Subject: [Histonet] Current issues with Printmates and Checkmates
In-Reply-To: <1298400300.254238.1290009880259.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>
References: <1298400300.254238.1290009880259.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>
Message-ID: <1B79F23C699F4168AAB37399DF84DC88@JoePC>

speaking of,
I guess my last posting rustled some feathers. I received a phone call from 
my rep saying that upper management wants to talk to me. I told him that 
there wasn't anything to talk about. Except that I was remiss. I want to 
thank Chris for all her work in the lab. I'm sorry that I didn't mention 
this before.
Any way back to the phone call- I told him that I said everything in my 
posting. No need to call me. At least this time I don't have to worry about 
my CEO being called. He's in Washington D.C.
----- Original Message ----- 
From: "Pamela Marcum" 
To: "Laurie Colbert" 
Cc: ; "Pamela Gholston" 

Sent: Wednesday, November 17, 2010 10:04 AM
Subject: Re: [Histonet] Current issues with Printmates and Checkmates




The ink also stays on through decal solutions which we needed. We do 30 bone 
marrows a day any system that could not stand up to decal was a no go for 
us.



Pam Marcum

UAMS





----- Original Message ----- 
From: "Laurie Colbert" 
To: "Pamela Gholston" , 
histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 17, 2010 9:10:19 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates

I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works
fine for us - the print is chemically resistant. We use xylene for both
processing and staining.

Laurie Colbert

-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela
Gholston
Sent: Tuesday, November 16, 2010 5:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Current issues with Printmates and Checkmates


Hello? Is anyone having issues with the Checkmate and/or Printmates?
What is the opinion out there about using the "hot foil tape
methodology?"
Is the tape chemically resistant?

Histopathy


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






----- Original Message ----- 
From: "Laurie Colbert" 
To: "Pamela Gholston" , 
histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 17, 2010 9:10:19 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates

I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works
fine for us - the print is chemically resistant. We use xylene for both
processing and staining.

Laurie Colbert

-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela
Gholston
Sent: Tuesday, November 16, 2010 5:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Current issues with Printmates and Checkmates


Hello? Is anyone having issues with the Checkmate and/or Printmates?
What is the opinion out there about using the "hot foil tape
methodology?"
Is the tape chemically resistant?

Histopathy


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From Jessica.Vacca <@t> HCAhealthcare.com  Thu Nov 18 06:38:35 2010
From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com)
Date: Thu Nov 18 06:38:49 2010
Subject: [Histonet] 10% Neutral Buffered Formalin - Methanol?
In-Reply-To: <4CE432BC.3060406@comcast.net>
References: <4AE8039AEA096143B965CBC6D092166802351B754B@EXCH2007.srhs-pa.org>
	<4CE405FF.6030505@umdnj.edu> <4CE432BC.3060406@comcast.net>
Message-ID: <938D716CD445614ABBB817517557B6F4F0FCFC52@NADCWPMSGCMS09.hca.corpad.net>

I have to say that we were just inspected by Florida AHCA-fire safety. We order Richard Allen 10% NBF-5 gallon cube, the inspector informed me that the formalin should be stored in a flammable cabinet. I of course told him that everywhere else I have worked I have never retrieved formalin from a flammable cabinet, so I got my MSDS and showed that in the storage and handling area of the MSDS there is nothing about storing w/ flammables-I was very proud of myself and willing to put up the fight.......However, he stated that because there is a "1" in the flammable section of the NFPA symbol located on the box-it classifies it as a Class 2b flammable/combustible liquid and therefore must be stored in a flammable cabinet. So money that could have gone to bigger and better things must now go towards a flammable cabinet. He also stated that NFPA out rules the MSDS........

Jessica Vacca
Histology Supervisor
Brandon Regional Hospital
813-571-6410

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Ray
Sent: Wednesday, November 17, 2010 2:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] 10% Neutral Buffered Formalin - Methanol?

The Methanol concentration of Formalin, 37% is frequently described on 
the MSDS as 10-15%.  However, I believe the actual Methanol 
concentration is  normally at the low end of that range.  Both 
Formaldehyde  Gas and Methanol are Flammable.

Commercial 10% NBF typically  contains about 1.1% Methanol and 3.7% 
Formaldehyde.  The  Flash Point of this water solution defines it as a 
Combustible Liquid.  It is not correct to call  it or handle as as a 
Flammable Liquid and it does not normally require Flammable Liquid 
storage and precautions.  Let the Safety Officers of your institution 
determine how you should handle and store these quantities of a 
Combustible Liquid.

Regards,
Mark



On 11/17/2010 10:42 AM, Geoff McAuliffe wrote:
> Commercially purchased 37% formaldehyde has had a small amount (about 
> 1.5% I think) of methanol added to it for many, many years. It helps 
> to prevent the polymerization of formaldehyde into insoluble 
> paraformaldehyde. It certainly does not make the stock solution 
> flammable and it is not contributing to drying out of your tissues. 
> Those who want methanol-free formalin make it from paraformaldehyde 
> but for LM there is no point.
>
> Geoff
>
>
> Jones, Laura wrote:
>> Greetings to all of you in Histoland.  Our lab recently switched from 
>> using a formalin substitute to using 10% Neutral Buffered Formalin.  
>> Our Pathologists have been unhappy with the small tissues, like GI 
>> biopsies and prostate cores.  They say they are seeing too much 
>> chatter and poor nuclear detail.  We have adjusted our processing 
>> times with only mildly better results.
>>
>> Today, I arrived at work to find staff cramming boxes and boxes of 
>> prefilled formalin vials into flame cabinets, as JCAHO is here this 
>> week.  It occurred to me that 10% NBF was not considered flammable 
>> when we used it years ago, and I was surprised to find that the MSDS 
>> for the bottles we had ordered listed methanol as an ingredient.  I 
>> immediately went back to my early days in Histo, when we made up 10% 
>> NBF ourselves from 37% concentrate; and we did not have any alcohol 
>> in our "recipe".  I thought I had discovered our whole problem!  
>> However, upon further research, we have found that most prefilled 
>> bottles DO contain methanol.  The large 20 litre cube, however does 
>> not list methanol as an ingredient.
>>
>> So, my questions are many.  Does that inclusion of methanol 
>> contribute to the drying out of tissues that we are seeing?  Does 
>> anyone sell prefilled bottles that contain methanol-free formalin?  
>> And, finally, does anyone have any other thoughts or suggestions?  I 
>> should add that we use Toluene as our clearing agent, because our 
>> former Pathologist believed it was less harsh on the tissues; and we 
>> are running our tissues on the Thermo Excelsior.  We are running 
>> small biopsies and large pieces of tissue together, which I know is 
>> not optimal, but we are a small hospital and one processor is it!  I 
>> am not a chemist and would appreciate any advice.  Thanks in advance.
>>
>>
>>
>>   ________________________________
>>
>> Sharon Regional Health System is the area's largest hospital
>> and provider of health care services. Visit us online at
>> http://www.sharonregional.com for a complete listing of our
>> services, primary care physicians and specialists, and satellite 
>> locations.
>>
>> Confidentiality Note: This message is intended for use
>> only by the individual or entity to which it is addressed
>> and may contain information that is privileged,
>> confidential, and exempt from disclosure under applicable
>> law. If the reader of this message is not the intended
>> recipient or the employee or agent responsible for
>> delivering the message to the intended recipient, you are
>> hereby notified that any dissemination, distribution or
>> copying of this communication is strictly prohibited. If
>> you have received this communication in error, please
>> contact the sender immediately and destroy the material in
>> its entirety, whether electronic or hard copy. Thank you.
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>
>


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From MW <@t> PersonifySearch.com  Thu Nov 18 07:35:49 2010
From: MW <@t> PersonifySearch.com (Matthew Ward)
Date: Thu Nov 18 07:42:37 2010
Subject: [Histonet] Histology Field Opportunities with a World Leader
Message-ID: <003301cb8725$83cc11c0$8b643540$@com>

Good Morning Everyone!! 

 

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(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

   www.personifysearch.com

 

From mpence <@t> grhs.net  Thu Nov 18 08:14:27 2010
From: mpence <@t> grhs.net (Mike Pence)
Date: Thu Nov 18 08:14:32 2010
Subject: [Histonet] 10% Neutral Buffered Formalin - Methanol?
In-Reply-To: <938D716CD445614ABBB817517557B6F4F0FCFC52@NADCWPMSGCMS09.hca.corpad.net>
Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974AA2@is-e2k3.grhs.net>

That is because NFPA has all of the safety companies that make safety
items in their back pocket.  I use to work for a safety company many
years ago.

Mike

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Jessica.Vacca@HCAhealthcare.com
Sent: Thursday, November 18, 2010 6:39 AM
To: darkdaym@comcast.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] 10% Neutral Buffered Formalin - Methanol?


I have to say that we were just inspected by Florida AHCA-fire safety.
We order Richard Allen 10% NBF-5 gallon cube, the inspector informed me
that the formalin should be stored in a flammable cabinet. I of course
told him that everywhere else I have worked I have never retrieved
formalin from a flammable cabinet, so I got my MSDS and showed that in
the storage and handling area of the MSDS there is nothing about storing
w/ flammables-I was very proud of myself and willing to put up the
fight.......However, he stated that because there is a "1" in the
flammable section of the NFPA symbol located on the box-it classifies it
as a Class 2b flammable/combustible liquid and therefore must be stored
in a flammable cabinet. So money that could have gone to bigger and
better things must now go towards a flammable cabinet. He also stated
that NFPA out rules the MSDS........

Jessica Vacca
Histology Supervisor
Brandon Regional Hospital
813-571-6410

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Ray
Sent: Wednesday, November 17, 2010 2:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] 10% Neutral Buffered Formalin - Methanol?

The Methanol concentration of Formalin, 37% is frequently described on 
the MSDS as 10-15%.  However, I believe the actual Methanol 
concentration is  normally at the low end of that range.  Both 
Formaldehyde  Gas and Methanol are Flammable.

Commercial 10% NBF typically  contains about 1.1% Methanol and 3.7% 
Formaldehyde.  The  Flash Point of this water solution defines it as a 
Combustible Liquid.  It is not correct to call  it or handle as as a 
Flammable Liquid and it does not normally require Flammable Liquid 
storage and precautions.  Let the Safety Officers of your institution 
determine how you should handle and store these quantities of a 
Combustible Liquid.

Regards,
Mark



On 11/17/2010 10:42 AM, Geoff McAuliffe wrote:
> Commercially purchased 37% formaldehyde has had a small amount (about
> 1.5% I think) of methanol added to it for many, many years. It helps 
> to prevent the polymerization of formaldehyde into insoluble 
> paraformaldehyde. It certainly does not make the stock solution 
> flammable and it is not contributing to drying out of your tissues. 
> Those who want methanol-free formalin make it from paraformaldehyde 
> but for LM there is no point.
>
> Geoff
>
>
> Jones, Laura wrote:
>> Greetings to all of you in Histoland.  Our lab recently switched from
>> using a formalin substitute to using 10% Neutral Buffered Formalin.  
>> Our Pathologists have been unhappy with the small tissues, like GI 
>> biopsies and prostate cores.  They say they are seeing too much 
>> chatter and poor nuclear detail.  We have adjusted our processing 
>> times with only mildly better results.
>>
>> Today, I arrived at work to find staff cramming boxes and boxes of
>> prefilled formalin vials into flame cabinets, as JCAHO is here this 
>> week.  It occurred to me that 10% NBF was not considered flammable 
>> when we used it years ago, and I was surprised to find that the MSDS 
>> for the bottles we had ordered listed methanol as an ingredient.  I 
>> immediately went back to my early days in Histo, when we made up 10% 
>> NBF ourselves from 37% concentrate; and we did not have any alcohol 
>> in our "recipe".  I thought I had discovered our whole problem!  
>> However, upon further research, we have found that most prefilled 
>> bottles DO contain methanol.  The large 20 litre cube, however does 
>> not list methanol as an ingredient.
>>
>> So, my questions are many.  Does that inclusion of methanol
>> contribute to the drying out of tissues that we are seeing?  Does 
>> anyone sell prefilled bottles that contain methanol-free formalin?  
>> And, finally, does anyone have any other thoughts or suggestions?  I 
>> should add that we use Toluene as our clearing agent, because our 
>> former Pathologist believed it was less harsh on the tissues; and we 
>> are running our tissues on the Thermo Excelsior.  We are running 
>> small biopsies and large pieces of tissue together, which I know is 
>> not optimal, but we are a small hospital and one processor is it!  I 
>> am not a chemist and would appreciate any advice.  Thanks in advance.
>>
>>
>>
>>   ________________________________
>>
>> Sharon Regional Health System is the area's largest hospital and 
>> provider of health care services. Visit us online at 
>> http://www.sharonregional.com for a complete listing of our services,

>> primary care physicians and specialists, and satellite locations.
>>
>> Confidentiality Note: This message is intended for use
>> only by the individual or entity to which it is addressed and may 
>> contain information that is privileged, confidential, and exempt from

>> disclosure under applicable law. If the reader of this message is not

>> the intended recipient or the employee or agent responsible for
>> delivering the message to the intended recipient, you are
>> hereby notified that any dissemination, distribution or
>> copying of this communication is strictly prohibited. If
>> you have received this communication in error, please
>> contact the sender immediately and destroy the material in
>> its entirety, whether electronic or hard copy. Thank you.
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>
>


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From lbustamante <@t> cvm.tamu.edu  Thu Nov 18 09:03:35 2010
From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante)
Date: Thu Nov 18 09:03:58 2010
Subject: [Histonet] Pancretic cell
Message-ID: <4CE4EBE7020000B9000E6B56@CVM.TAMU.EDU>

Does anyone knows any good, clean Antibody for Pancreatic cells alpha,
beta and delta? One for each or a combination of them? We are going to
start using dog tissue.
This work will be entirely for teaching purposes.
If you have any protocol I will be happy to have it too.
Thank you.
Lin.

Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab Supervisor
Veterinary Integrative Bioscience
Texas A&M University
College Station, TX 77843-4458
From SAllen <@t> exchange.hsc.mb.ca  Thu Nov 18 09:06:28 2010
From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen)
Date: Thu Nov 18 09:06:51 2010
Subject: [Histonet] FW: fixing whole brains
Message-ID: 



_____________________________________________
From: Sharon Allen 
Sent: November 18, 2010 8:08 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: fixing whole brains

Hi,
I would like to know what percentage of formalin Neuro Labs out there
use to fix their whole brains before cutup. We do just under a1000
brains a year & have always used 10% neutral buffered formalin for 2
weeks, then do brain cutting & place the cassettes into 70% alcohol
overnight then on the processor into 95% alcohol.  This has worked well.
But!!  We have been getting brains from outside our facility which have
been falling apart almost immediately in the waterbath. It is only the
brains from this facility that we have the problem with.  From the time
of brain cutting, through the processing, embedding & cutting, all
cassettes are handled the same, the only difference we can find is that
they put their whole brains into 20% formalin for 2 weeks & sometimes 6
weeks before we receive them.  Does anyone else use 20% formalin, & are
there any extra steps required for processing these ones or have any
suggestions why we are having this problem?  
Any help would be greatly appreciated.
Thanks
Sharon Allen
Neuropathology Lab
sallen@hsc.mb.ca
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From shive003 <@t> umn.edu  Thu Nov 18 09:34:06 2010
From: shive003 <@t> umn.edu (shive003@umn.edu)
Date: Thu Nov 18 09:34:10 2010
Subject: [Histonet] Pancretic cell
In-Reply-To: <4CE4EBE7020000B9000E6B56@CVM.TAMU.EDU>
References: <4CE4EBE7020000B9000E6B56@CVM.TAMU.EDU>
Message-ID: 

I use antibodies which work on each cell type (they do work on dog tissue):

xGlucagon (for alpha cells); Dako; cat. # A0565
xInsulin (for beta cells); Dako; cat. # A0564
xSomatostatin (for delta cells); Dako; cat. # A0566

Jan Shivers
Senior Scientist
IHC/Histology/EM Section Head
Univ. of Minnesota Veterinary Diagnostic Lab
St. Paul, MN


On Nov 18 2010, Lin Bustamante wrote:

>Does anyone knows any good, clean Antibody for Pancreatic cells alpha,
>beta and delta? One for each or a combination of them? We are going to
>start using dog tissue.
>This work will be entirely for teaching purposes.
>If you have any protocol I will be happy to have it too.
>Thank you.
>Lin.
>
>Lin S. Bustamante, B.Sc.; HT(ASCP)
>Research Associate
>Histology Lab Supervisor
>Veterinary Integrative Bioscience
>Texas A&M University
>College Station, TX 77843-4458
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

From sadey <@t> hotmail.ca  Thu Nov 18 09:42:23 2010
From: sadey <@t> hotmail.ca (Sheila Adey)
Date: Thu Nov 18 09:42:27 2010
Subject: [Histonet] Cassette and slide labellers
Message-ID: 


Hello
We are looking to purchase a cassette and slide labeller system. We are located in Sarnia Ontario Canada. Could reps for these items contact us at 519-464-4400 ext. 5277 ask for Sharon. 
We would also love to hear other peoples opinions on which systems you recommend. 
Thanks. 
Sheila 		 	   		  
From tenfelde <@t> polysciences.com  Thu Nov 18 10:35:33 2010
From: tenfelde <@t> polysciences.com (Jennifer Tenfelde)
Date: Thu Nov 18 10:35:38 2010
Subject: [Histonet] (no subject)
Message-ID: 

Great job opportunity at Polysciences located in the Philadelphia area!! 
 
We're seeking a full-time Biosciences Product Specialist to assist in
development of new products, perform quality control/assurance, conduct
competitive benchmarking, and provide customer technical support for the
histology and broader biosciences market segments. This is an awesome
opportunity for someone who wants to still work in the lab but develop a
skill-set that exceeds the typical lab boundaries!
 
The Biosciences Product Specialist will:
 
?    Assists in the development of new products for the histology and
biosciences markets by formulating new products, testing the new products,
and benchmarking the new products against market leading competing products.
Creates concise summaries of performance testing results.

?    Performs quality control testing on histology and biosciences products
that require performance testing as a key measure of quality.  Also
troubleshoots complaints regarding product quality and develops test
protocols as required.

?    Tests certain existing Polysciences biosciences products against market
leading competitive products to generate comparison data of certain
Polysciences products relative to the competition, in order to provide
additional information for sales tools and to develop ideas for product
improvements where necessary.  Creates concise summaries of the testing
results.  Contributes to the improvement of existing products where deemed
appropriate.

?    Provides technical support by phone and email to customers with
questions about histology and biosciences products.  Follows up with
potential customers by phone when appropriate to facilitate sales
opportunities.

?    Maintain safe working environment and associated laboratory equipment
required for bioscience product testing and development, including
coordinating preventative maintenance for various pieces of equipment.

 

 

The ideal candidate will have experience in all phases of light and electron
microscopy with professional registration in the field. Experience with
processing, sectioning, and staining tissues for histology and electron
microscopy is required. We need someone with an outgoing personality and
interpersonal skills that will facilitate cross-functional teamwork.
Computer literacy including Microsoft Excel, Word, and PowerPoint are a
must. HT and HT/HTL (ASCP) with BS in Chemistry, Biochemistry, Molecular
Biology, or related Biosciences degree required. Minimum 3-5 years work
experience (post grad) with histology and biosciences products in a
laboratory environment ? research experience preferred.

 

We offer a competitive salary and a comprehensive benefits package including
health, dental, life, supplemental life, short & long term disability,
flexible spending accounts, 401(k) with healthy company match, college
savings plan, long-term care insurance, and more!

 

Interested in applying? Please send your resume to hr@polysciences.com.

 

Check out our website: www.polysciences.com!

 

EOE. M/F/D/V. Drug-free workplace.

 

 

Jennifer L. Tenfelde, SPHR
Human Resource Manager

Polysciences, Inc. 
400 Valley Road 
Warrington, PA 18976 
Phone:  215 - 488 - 7525 
Fax:    215 - 343 -  0865 

 
From Valerie.Hannen <@t> parrishmed.com  Thu Nov 18 10:51:03 2010
From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie)
Date: Thu Nov 18 10:51:10 2010
Subject: [Histonet] Metal detectors
Message-ID: <5680DA93771F0C48954CC8D38425E72401AB3517@ISMAIL.parrishmed.local>

Hi folks... I am sending this message on behalf of my section chief. She
is wondering if anyone has any information on where we can get/buy a
small (possibly pencil size) metal detector? Sometimes our Pathologists
are not diligent about getting all of the staples out of the tissue
samples that they give us...and yes...that messes up our sectioning
blades when we are cutting the days slides. Any help will be greatly
appreciated.
 
Thanks!
 
Valerie Hannen, Histotechnologist
Parrish Medical Center
Titusville, Florida


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privileged, confidential or otherwise exempt from disclosure
under applicable law. If the reader of this email is not the
intended recipient or the employee or agent responsible for
delivering the message to the intended recipient, you are
hereby notified that any dissemination, distribution, or
copying of this communication is strictly prohibited. If you
have received this communication in error, please immediately
delete this message. Thank you"
**************************************************************
From mhale <@t> carisls.com  Thu Nov 18 11:09:58 2010
From: mhale <@t> carisls.com (Hale, Meredith)
Date: Thu Nov 18 11:10:04 2010
Subject: [Histonet] HT Opprotunity
Message-ID: <6F33D8418806044682A391273399860F06044A14@s-irv-ex301.PathologyPartners.intranet>

Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time  position
that offers a competitive salary and  the flexible hours allows you to
put your own personal stamp on the laboratory .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mhale@carisls.com

 

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mhale@carisls.com   

 

From gagnone <@t> KGH.KARI.NET  Thu Nov 18 11:25:57 2010
From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric)
Date: Thu Nov 18 11:26:10 2010
Subject: [Histonet] Metal detectors
Message-ID: 

This is an interesting idea, Valerie, and I too will be interested in any responses.  Such a detector would certainly reduce the number of "I couldn't see the staples" responses we get when we ask our Pathologist's Assistants about these problematic sections.  Another question I'd have is whether the detector can detect all sizes of surgical staples: the large ones and the multiple tiny ones which tend to be used in greater numbers and are harder to detect.
 
Now if we could only find a suture detector...
 
Thanks in advance,
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada


From mpence <@t> grhs.net  Thu Nov 18 11:33:47 2010
From: mpence <@t> grhs.net (Mike Pence)
Date: Thu Nov 18 11:33:53 2010
Subject: [Histonet] Current issues with Printmates and Checkmates
In-Reply-To: <1B79F23C699F4168AAB37399DF84DC88@JoePC>
Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974AA5@is-e2k3.grhs.net>

If things are as you stated you should want to talk with their upper
management.

Mike

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: Wednesday, November 17, 2010 5:54 PM
To: Pamela Marcum; Laurie Colbert
Cc: histonet@lists.utsouthwestern.edu; Pamela Gholston
Subject: Re: [Histonet] Current issues with Printmates and Checkmates


speaking of,
I guess my last posting rustled some feathers. I received a phone call
from 
my rep saying that upper management wants to talk to me. I told him that

there wasn't anything to talk about. Except that I was remiss. I want to

thank Chris for all her work in the lab. I'm sorry that I didn't mention

this before.
Any way back to the phone call- I told him that I said everything in my 
posting. No need to call me. At least this time I don't have to worry
about 
my CEO being called. He's in Washington D.C.
----- Original Message ----- 
From: "Pamela Marcum" 
To: "Laurie Colbert" 
Cc: ; "Pamela Gholston" 

Sent: Wednesday, November 17, 2010 10:04 AM
Subject: Re: [Histonet] Current issues with Printmates and Checkmates




The ink also stays on through decal solutions which we needed. We do 30
bone 
marrows a day any system that could not stand up to decal was a no go
for 
us.



Pam Marcum

UAMS





----- Original Message ----- 
From: "Laurie Colbert" 
To: "Pamela Gholston" , 
histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 17, 2010 9:10:19 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates

I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works
fine for us - the print is chemically resistant. We use xylene for both
processing and staining.

Laurie Colbert

-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela
Gholston
Sent: Tuesday, November 16, 2010 5:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Current issues with Printmates and Checkmates


Hello? Is anyone having issues with the Checkmate and/or Printmates?
What is the opinion out there about using the "hot foil tape
methodology?" Is the tape chemically resistant?

Histopathy


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






----- Original Message ----- 
From: "Laurie Colbert" 
To: "Pamela Gholston" , 
histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 17, 2010 9:10:19 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates

I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works
fine for us - the print is chemically resistant. We use xylene for both
processing and staining.

Laurie Colbert

-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela
Gholston
Sent: Tuesday, November 16, 2010 5:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Current issues with Printmates and Checkmates


Hello? Is anyone having issues with the Checkmate and/or Printmates?
What is the opinion out there about using the "hot foil tape
methodology?" Is the tape chemically resistant?

Histopathy


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From POWELL_SA <@t> mercer.edu  Thu Nov 18 13:04:04 2010
From: POWELL_SA <@t> mercer.edu (Shirley A. Powell)
Date: Thu Nov 18 13:04:12 2010
Subject: [Histonet] RE: Metal detectors
In-Reply-To: 
References: 
Message-ID: <9BF995BC0E47744E9673A41486E24EE226924427F3@MERCERMAIL.MercerU.local>

A small stud finder should work.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric
Sent: Thursday, November 18, 2010 12:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Metal detectors

This is an interesting idea, Valerie, and I too will be interested in any responses.  Such a detector would certainly reduce the number of "I couldn't see the staples" responses we get when we ask our Pathologist's Assistants about these problematic sections.  Another question I'd have is whether the detector can detect all sizes of surgical staples: the large ones and the multiple tiny ones which tend to be used in greater numbers and are harder to detect.
 
Now if we could only find a suture detector...
 
Thanks in advance,
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada


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From histology <@t> medsurgpath.com  Thu Nov 18 13:28:25 2010
From: histology <@t> medsurgpath.com (Katelin Lester)
Date: Thu Nov 18 13:28:30 2010
Subject: [Histonet] Patients' Pathology Requisitions
In-Reply-To: 
References: 
Message-ID: <06d945544a243c929808459016b6db90.squirrel@webmail.integra.net>

2 years for us as well

Katelin Lester, HTL(ASCP)
MedSurg Pathology Associates, Inc.
(503)443-2157

>
> Hello everyone,
>
> Could someone please tell how long we are required to keep the patients'
> pathology requisitions in the lab, after the case has been read or signed
> out.
>
> Thank You! 		 	   		  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



From jnocito <@t> satx.rr.com  Thu Nov 18 16:36:16 2010
From: jnocito <@t> satx.rr.com (Joe Nocito)
Date: Thu Nov 18 16:36:28 2010
Subject: [Histonet] Current issues with Printmates and Checkmates
In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974AA5@is-e2k3.grhs.net>
References: <661949901A768E4F9CC16D8AF8F2838C03974AA5@is-e2k3.grhs.net>
Message-ID: 

there is nothing to talk about. There was a problem, the company fixed it. End of story. I don't understand the fuss. But alas! I did receive another phone call at work today. Which prompted my supervisor to remind me that I must include a disclaimer. So here is my disclaimer

The expressed opinions and views in this transmission are those solely of the author. They are in now way, shape, or form representative views of the U.S. Air Force, U.S. government, the President of the United State, the Speaker of the House, the Minority Whip, my lawyers, their lawyers, your lawyers and their future off spring.

Unlike my other employment situation where I was on the verge of being fired (remember the good old days when I was forbidden from the Histonet?)  the current situation allows me to speak my mind, that is of course if I add the disclaimer. Then anything and everyone is open season. Which reminds me, those of you who live in a tourist town, how come when it's tourist season, you can't shoot them? There's deer season, bird season, crab season and tourist season. Just a thought.

----- Original Message ----- 
From: "Mike Pence" 
To: "Joe Nocito" ; "Pamela Marcum" ; "Laurie Colbert" 
Cc: ; "Pamela Gholston" 
Sent: Thursday, November 18, 2010 11:33 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates


If things are as you stated you should want to talk with their upper
management.

Mike

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: Wednesday, November 17, 2010 5:54 PM
To: Pamela Marcum; Laurie Colbert
Cc: histonet@lists.utsouthwestern.edu; Pamela Gholston
Subject: Re: [Histonet] Current issues with Printmates and Checkmates


speaking of,
I guess my last posting rustled some feathers. I received a phone call
from 
my rep saying that upper management wants to talk to me. I told him that

there wasn't anything to talk about. Except that I was remiss. I want to

thank Chris for all her work in the lab. I'm sorry that I didn't mention

this before.
Any way back to the phone call- I told him that I said everything in my 
posting. No need to call me. At least this time I don't have to worry
about 
my CEO being called. He's in Washington D.C.
----- Original Message ----- 
From: "Pamela Marcum" 
To: "Laurie Colbert" 
Cc: ; "Pamela Gholston" 

Sent: Wednesday, November 17, 2010 10:04 AM
Subject: Re: [Histonet] Current issues with Printmates and Checkmates




The ink also stays on through decal solutions which we needed. We do 30
bone 
marrows a day any system that could not stand up to decal was a no go
for 
us.



Pam Marcum

UAMS





----- Original Message ----- 
From: "Laurie Colbert" 
To: "Pamela Gholston" , 
histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 17, 2010 9:10:19 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates

I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works
fine for us - the print is chemically resistant. We use xylene for both
processing and staining.

Laurie Colbert

-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela
Gholston
Sent: Tuesday, November 16, 2010 5:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Current issues with Printmates and Checkmates


Hello? Is anyone having issues with the Checkmate and/or Printmates?
What is the opinion out there about using the "hot foil tape
methodology?" Is the tape chemically resistant?

Histopathy


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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






----- Original Message ----- 
From: "Laurie Colbert" 
To: "Pamela Gholston" , 
histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 17, 2010 9:10:19 AM
Subject: RE: [Histonet] Current issues with Printmates and Checkmates

I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works
fine for us - the print is chemically resistant. We use xylene for both
processing and staining.

Laurie Colbert

-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela
Gholston
Sent: Tuesday, November 16, 2010 5:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Current issues with Printmates and Checkmates


Hello? Is anyone having issues with the Checkmate and/or Printmates?
What is the opinion out there about using the "hot foil tape
methodology?" Is the tape chemically resistant?

Histopathy


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


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From Timothy.Morken <@t> ucsfmedctr.org  Thu Nov 18 18:30:33 2010
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim)
Date: Thu Nov 18 18:30:43 2010
Subject: [Histonet] prostate orientation
Message-ID: 

I would like to hear about whatever system you may have for orienting prostate needle biopsies straight and handling from bx through embedding.


I'd especially like to know if anyone is using the procedure outlined in this paper:

"Optimized preembedding method improves the histologic yield of prostatic core needle biopsies," Rogatsch, H et al, The Prostate, 42:124-129 (2000).

Thanks ahead of time!

Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center

From akemiat3377 <@t> yahoo.com  Thu Nov 18 18:57:50 2010
From: akemiat3377 <@t> yahoo.com (Akemi Allison)
Date: Thu Nov 18 18:57:56 2010
Subject: [Histonet] prostate orientation
In-Reply-To: 
References: 
Message-ID: <44CDA7F3-1D34-4FB2-B57E-7E0EF0E8045B@yahoo.com>

Tim,

Helen Fedor has had a tremendous amount of experience on this subject  
since she is the SPORE lab manager for Johns Hopkins.  Johns Hopkins  
is considered the go to facility for prostate technology.  I have  
CC'd Helen on the email communication.

Best Wishes for the Holidays
Akemi

Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377@yahoo.com

On Nov 18, 2010, at 4:30 PM, Morken, Tim wrote:

> I would like to hear about whatever system you may have for  
> orienting prostate needle biopsies straight and handling from bx  
> through embedding.
>
>
> I'd especially like to know if anyone is using the procedure  
> outlined in this paper:
>
> "Optimized preembedding method improves the histologic yield of  
> prostatic core needle biopsies," Rogatsch, H et al, The Prostate,  
> 42:124-129 (2000).
>
> Thanks ahead of time!
>
> Tim Morken
> Supervisor, Histology, IPOX
> UC San Francisco Medical Center
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Beth.Fye <@t> HCAhealthcare.com  Thu Nov 18 19:10:19 2010
From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com)
Date: Thu Nov 18 19:10:24 2010
Subject: [Histonet] Rusting in Pathology Department
Message-ID: <938F8EC5A524D34EB5796E23E52781D34EA013B18D@NADCWPMSGCMS05.hca.corpad.net>

Here is a question I don't believe I ever seen discussed here before.  Does anyone else have significant rusting issues in their Histology Departments?  Cabinet hinges, metal outlet plates, printers, etc. are rusting in our department, especially around the microtome areas.  Please let me know if you are having any of these issues, or can think of any reason for this.  We had a printer in the lab that the whole interior rusted out.  This is not minor rusting that I could contribute to water bath  humidity.

Beth A. Fye, CT (ASCP)
Pathology Technical  Manager
HCA Richmond Hospital Laboratories
office:  (804)228-6564
fax: (804)323-8638





From JMacDonald <@t> mtsac.edu  Thu Nov 18 19:38:46 2010
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Thu Nov 18 19:38:50 2010
Subject: [Histonet] Rusting in Pathology Department
In-Reply-To: <938F8EC5A524D34EB5796E23E52781D34EA013B18D@NADCWPMSGCMS05.hca.corpad.net>
Message-ID: 

Is decal solution being stored in any of those cabinets or drawers?  Some 
techs will have a container that they keep near their microtomes for 
surface decal. 




 
Sent by: histonet-bounces@lists.utsouthwestern.edu
11/18/2010 05:13 PM

To

cc

Subject
[Histonet] Rusting in Pathology Department






Here is a question I don't believe I ever seen discussed here before. Does 
anyone else have significant rusting issues in their Histology 
Departments?  Cabinet hinges, metal outlet plates, printers, etc. are 
rusting in our department, especially around the microtome areas.  Please 
let me know if you are having any of these issues, or can think of any 
reason for this.  We had a printer in the lab that the whole interior 
rusted out.  This is not minor rusting that I could contribute to water 
bath  humidity.

Beth A. Fye, CT (ASCP)
Pathology Technical  Manager
HCA Richmond Hospital Laboratories
office:  (804)228-6564
fax: (804)323-8638





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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From histologydirector <@t> gmail.com  Fri Nov 19 07:36:20 2010
From: histologydirector <@t> gmail.com (Histology Director)
Date: Fri Nov 19 07:36:25 2010
Subject: [Histonet] GU Lab in Columbus
Message-ID: 

Looking for experienced histology technicians and cytotechnologists in
Columbus, Ohio for new GU pathology laboratory. Excellent compensation with
great benefits. Experience with IHC, FISH, and GU pathology a plus. Send
resume or CV to careers@aksm.com.
From brian <@t> prometheushealthcare.com  Fri Nov 19 08:24:16 2010
From: brian <@t> prometheushealthcare.com (Brian- Prometheus)
Date: Fri Nov 19 08:24:30 2010
Subject: [Histonet] Histology Supervisor Morning Shift Opening 
 Histology Supervisor Morning Shift Opening
Message-ID: <007801cb87f5$75b4c6b0$611e5410$@com>

Private Histology Lab in Baton Rouge, Louisiana is seeking an experienced
working supervisor for the early morning shift.

Please contact me today for immediate consideration.

Thanks!

 

Responsibilities:

-Supervision of 10 histology technicians and 2 lab assistants

-Assists Histo Techs with morning work - sectioning and staining

-Prioritizes day to day workflow and ensures standards of accuracy, quality,
and turn around times are met

-Adjusts schedules and provides input for assessment or disciplinary action
as needed

Experience: 

Previous supervisory, IHC and Special Staining experience is required.

Qualifications:

HTL or HT (ASCP) certification required

2 years previous supervisory experience preferred

 

 

They offer a competitive benefit package, including 401K plan. Salary is
commensurate with experience.

 

Brian Feldman

Principal

Prometheus Healthcare 

Office 301-693-9057

Fax 301-368-2478

brian  @prometheushealthcare.com

  www.prometheushealthcare.com

*** Stay up to date on the newest positions and healthcare trends nationwide
on Twitter!***

   http://twitter.com/PrometheusBlog

 

From gagnone <@t> KGH.KARI.NET  Fri Nov 19 08:33:04 2010
From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric)
Date: Fri Nov 19 08:33:19 2010
Subject: [Histonet] Rusting in Pathology Department
Message-ID: 

In our laboratory, we have also experienced this, especially with microtomes.  As Jennifer has said, it seemed decal solution containing RDO was the culprit, when used in proximity to microtomes.  Techs who used RDO for surface decalcification had more rusting on exposed metal parts of the microtome, probably due to incomplete rinsing of the block after immersion in the RDO.  Parts of the microtomes not exposed, where the acid seems to have been penetrated, included locking mechanisms and under the blade clamp.  I would suggest anyone using RDO surface decal do so by immersing the block in a shallow dish of RDO (square Coplin jar lid works well).  We now store RDO away from the microtomes.
 
We have no data on how the techs may have been rusting as well.  Effects may rival fixation of techs by close proximity to formalin x years. It's Friday :)
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital
Kingston, Ontario, Canada
 
 
 


From campbellj <@t> muhlbauerlab.com  Fri Nov 19 08:44:26 2010
From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell)
Date: Fri Nov 19 08:44:30 2010
Subject: [Histonet] job opening
Message-ID: 

Histonetters,

Private dermpath lab in central/western NY is looking for a fully licensed
(in NYS) histotech for a full time position.

Please contact me directly by email or phone.



-- 
Jen Campbell
Supervisor of Technical Services
Muhlbauer Dermatopathology Laboratory
P: 585.586.5166
F: 585.586.3137
From BSullivan <@t> shorememorial.org  Fri Nov 19 08:52:32 2010
From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org)
Date: Fri Nov 19 08:55:32 2010
Subject: [Histonet] Rusting in Pathology Department
In-Reply-To: 
Message-ID: 

Levity on Friday. Thank you.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


Speak only well of people and you need never whisper


                                                                           
             "Gagnon, Eric"                                                
                                                                   To 
             Sent by:                   
             histonet-bounces@                                          cc 
             lists.utsouthwest                                             
             ern.edu                                               Subject 
                                       [Histonet] Rusting in Pathology     
                                       Department                          
             11/19/2010 09:33                                              
             AM                                                            
                                                                           
                                                                           
                                                                           
                                                                           




In our laboratory, we have also experienced this, especially with
microtomes.  As Jennifer has said, it seemed decal solution containing RDO
was the culprit, when used in proximity to microtomes.  Techs who used RDO
for surface decalcification had more rusting on exposed metal parts of the
microtome, probably due to incomplete rinsing of the block after immersion
in the RDO.  Parts of the microtomes not exposed, where the acid seems to
have been penetrated, included locking mechanisms and under the blade
clamp.  I would suggest anyone using RDO surface decal do so by immersing
the block in a shallow dish of RDO (square Coplin jar lid works well).  We
now store RDO away from the microtomes.

We have no data on how the techs may have been rusting as well.  Effects
may rival fixation of techs by close proximity to formalin x years. It's
Friday :)

Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital
Kingston, Ontario, Canada





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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From JWeems <@t> sjha.org  Fri Nov 19 09:25:36 2010
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Fri Nov 19 09:25:42 2010
Subject: [Histonet] RE: Rusting in Pathology Department
In-Reply-To: 
References: 
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044A0FAFCD@CHEXCMS10.one.ads.che.org>

My 98 yr old mom says the golden years are pretty rusty sometimes. I guess we can just get a head start! J:>) 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric
Sent: Friday, November 19, 2010 09:33
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rusting in Pathology Department

In our laboratory, we have also experienced this, especially with microtomes.  As Jennifer has said, it seemed decal solution containing RDO was the culprit, when used in proximity to microtomes.  Techs who used RDO for surface decalcification had more rusting on exposed metal parts of the microtome, probably due to incomplete rinsing of the block after immersion in the RDO.  Parts of the microtomes not exposed, where the acid seems to have been penetrated, included locking mechanisms and under the blade clamp.  I would suggest anyone using RDO surface decal do so by immersing the block in a shallow dish of RDO (square Coplin jar lid works well).  We now store RDO away from the microtomes.
 
We have no data on how the techs may have been rusting as well.  Effects may rival fixation of techs by close proximity to formalin x years. It's Friday :)
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital
Kingston, Ontario, Canada
 
 
 


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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This e-mail, including any attachments is the 
property of Catholic Health East and is intended 
for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please delete this message, and 
reply to the sender regarding the error in a separate email. 
 


From micropathlabs <@t> yahoo.com  Fri Nov 19 10:16:35 2010
From: micropathlabs <@t> yahoo.com (Sheila Haas)
Date: Fri Nov 19 10:16:39 2010
Subject: [Histonet] Modified GMS
Message-ID: <24062.7620.qm@web57806.mail.re3.yahoo.com>

Hi all. Years ago, I used a modified GMS procedure that used?periodic acid in 
place of chromic acid. Would anyone be willing to share their procedure?for this 
or at least a vendor you might be purchasing a kit from? Thanks in advance!?
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.


      
From relia1 <@t> earthlink.net  Fri Nov 19 10:17:00 2010
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Fri Nov 19 10:17:04 2010
Subject: [Histonet] RELIA Histology Job Alert 11/18/10
Message-ID: 

  
Hi Histonetters!
I hope everyone is having a great day.  I wanted to take just a minute
of your time to tell you about some histology opportunities that I am
working on.  All of my positions are full time permanent jobs.  My
clients offer excellent compensation, benefits, relocation assistance
and great places to work.
I know that as the holidays draw closer a job change is one of the last
things on most people's minds however my clients are willing to move
right away or work with you after the holidays whichever you prefer.
 
Here is a list of my current openings:
MANAGEMENT
LA - Histology Supervisor
AR ? Director of Core Lab
CA ? Histology Lab Manager
NY-Quality Assurance Manager
 
HISTOTECHNICIANS/HISTOTECHNOLOGISTS
MD- Near Hagerstown day shift state of the art lab
TN ? Nashville Histotech 2 night shift positions avail.
TN - Chattanooga Night Shift Grossing histotech
FL- Central Florida Histology Product Sales
IN ? South of Chicago in Northern IN Histology Tech
GA ? Southern Coastal area ? Histotech with IHC ASCP HTL required.
NY-    Suffern NYS license/Elig day and night shift 
MA ? Boston Area several shifts available dermpath and general
FL ? Orlando State of FLTechnologist license and 2 yrs exper req.
CA - Tech Support/Inside Sales strong background in TEM
 
I realize that there are a lot of recruiters out there contacting you
about opportunities and I want to remind you that I am the only
recruiter who offers you over 25 years of experience helping people find
the right position at the right time and 9 years exclusively in
histology.  I offer a practice that works exclusively in the nationwide
permanent placement of histology professionals.  Your job search will be
confidential and tailored specifically to your needs.
 
If you or anyone you know might be interested in a new opportunity
please contact me toll free at 866-607-3542 or relia1@earthlink.net  
 
Thanks-Pam

Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From gayle.callis <@t> bresnan.net  Fri Nov 19 10:26:04 2010
From: gayle.callis <@t> bresnan.net (gayle callis)
Date: Fri Nov 19 10:26:12 2010
Subject: [Histonet] RE: Rusting in histology department
Message-ID: <000001cb8806$781a1e30$684e5a90$@callis@bresnan.net>

RDO has a high concentration of hydrochloric acid, the source of acid fumes
that corrode metals.  It may not be necessary to use concentrated
decalcifier e.g. RDO or any other HCL based decalcifier for effective
surface decalcification and I suggest diluting this with water to help
minimize fumes.  I also suggest using an enclosed petri dish or plastic
Tupperware container to do this type of decalcification.    

 

You may want to store your decalcifying solution in a plastic container with
lid, as we did with concentrated stock HCl.  Concentrated HCl used to be
shipped in gallon size plastic jug holders so fumes were pretty much
contained, and we had no corrosion in our lab.  One lab recently had to move
their acid from under a sink with metal plumbing parts due to severe
corrosion, or use an acid storage cabinet which very well may be required by
some facilities. 

 

DMSO also causes rusting and we noticed Paraplast plus that contains DMSO
caused rusting of metal parts in an incubator oven and equipment solenoids.
We also did not like the funny taste in our mouths when working with melted
Paraplast plus since the solvent fumes penetrates through skin and membranes
so easily.  We stopped using this  paraffin because of these reasons.   The
paraffin is supposedly effective for better penetration during infiltration
but exposure to fumes was a factor and we now use vacuum/pressure automated
processing to  improve paraffin infiltration   

 

Gayle M. Callis

HTL/HT/MT(ASCP)

 

From godsgalnow <@t> aol.com  Fri Nov 19 10:27:49 2010
From: godsgalnow <@t> aol.com (godsgalnow@aol.com)
Date: Fri Nov 19 10:28:00 2010
Subject: [Histonet] Nails
Message-ID: <8CD561DD725D8AE-14E0-4503@webmail-d035.sysops.aol.com>


We are having a very difficault time with the naisl falling off the slides when we do the PASF stain.  We are using charged slides already and we have tried increasing the baking time during drying to no avail.

Any suggestions?


Roxanne



From micropathlabs <@t> yahoo.com  Fri Nov 19 10:34:58 2010
From: micropathlabs <@t> yahoo.com (Sheila Haas)
Date: Fri Nov 19 10:35:02 2010
Subject: [Histonet] Nails
In-Reply-To: <8CD561DD725D8AE-14E0-4503@webmail-d035.sysops.aol.com>
References: <8CD561DD725D8AE-14E0-4503@webmail-d035.sysops.aol.com>
Message-ID: <707430.71731.qm@web57805.mail.re3.yahoo.com>

We've had recent trouble with our sections on Superfrost Plus slides from 
Cardinal. Not sure what brand you're using, but might try a different company.
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
?




________________________________
From: "godsgalnow@aol.com" 
To: histonet@lists.utsouthwestern.edu
Sent: Fri, November 19, 2010 11:27:49 AM
Subject: [Histonet] Nails


We are having a very difficault time with the naisl falling off the slides when 
we do the PASF stain.? We are using charged slides already and we have tried 
increasing the baking time during drying to no avail.

Any suggestions?


Roxanne



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From POWELL_SA <@t> mercer.edu  Fri Nov 19 10:40:20 2010
From: POWELL_SA <@t> mercer.edu (Shirley A. Powell)
Date: Fri Nov 19 10:40:27 2010
Subject: [Histonet] Nails
In-Reply-To: <8CD561DD725D8AE-14E0-4503@webmail-d035.sysops.aol.com>
References: <8CD561DD725D8AE-14E0-4503@webmail-d035.sysops.aol.com>
Message-ID: <9BF995BC0E47744E9673A41486E24EE22692442D9A@MERCERMAIL.MercerU.local>

Switch to Stay On and plain slides, I had problems with charged slides and now have good results with the additive and regular slides.  Cheaper in the long run too.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com
Sent: Friday, November 19, 2010 11:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nails


We are having a very difficault time with the naisl falling off the slides when we do the PASF stain.  We are using charged slides already and we have tried increasing the baking time during drying to no avail.

Any suggestions?


Roxanne



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From lblazek <@t> digestivespecialists.com  Fri Nov 19 10:50:40 2010
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Fri Nov 19 10:50:45 2010
Subject: [Histonet] Nails
In-Reply-To: <9BF995BC0E47744E9673A41486E24EE22692442D9A@MERCERMAIL.MercerU.local>
References: <8CD561DD725D8AE-14E0-4503@webmail-d035.sysops.aol.com>
	<9BF995BC0E47744E9673A41486E24EE22692442D9A@MERCERMAIL.MercerU.local>
Message-ID: <5A2BD13465E061429D6455C8D6B40E390EB42CE46C@IBMB7Exchange.digestivespecialists.com>

An old old trick is taking a plain slide and coating it with dilute Elmer's glue and letting it dry. Be quick picking it up from the water bath and let dry well.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell
Sent: Friday, November 19, 2010 11:40 AM
To: godsgalnow@aol.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Nails

Switch to Stay On and plain slides, I had problems with charged slides and now have good results with the additive and regular slides.  Cheaper in the long run too.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com
Sent: Friday, November 19, 2010 11:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nails


We are having a very difficault time with the naisl falling off the slides when we do the PASF stain.  We are using charged slides already and we have tried increasing the baking time during drying to no avail.

Any suggestions?


Roxanne



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From ross <@t> premierlab.com  Fri Nov 19 11:02:58 2010
From: ross <@t> premierlab.com (Ross Benik)
Date: Fri Nov 19 10:59:55 2010
Subject: [Histonet] (no subject)
Message-ID: 

Hello everyone, 

 

Has anyone ever attempted to use the Leukocyte Alkaline Phosphatase Kit
from Sigma (see link below) on FFPE tissues? If so can you confirm that
it does or does not work? 

 

Thanks, 

Ross 

 

http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=86C|SIGM
A&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC

From Maria.Katleba <@t> stjoe.org  Fri Nov 19 11:17:32 2010
From: Maria.Katleba <@t> stjoe.org (Maria Katleba)
Date: Fri Nov 19 11:18:41 2010
Subject: [Histonet] Nails
In-Reply-To: <8CD561DD725D8AE-14E0-4503@webmail-d035.sysops.aol.com>
References: <8CD561DD725D8AE-14E0-4503@webmail-d035.sysops.aol.com>
Message-ID: 

How To Cut Nails
1. section nail and place on superfrost plus (or adhesive slide)
2. shake excess water off slide
3. place 1-3 drop formaldehyde (10% buffered ok) at the bottom of a plastic coplin jar
4. place cut slides in jar
5. tighten cap
6.Place on hot over 60 degrees...15 -20 minutes
7. remove (do NOT rinse) and cool
8. stain or deparifinize

The aldehyde in the formalin moves through the tissue and with the heat, it vaporizes... it pushes the tissue against the glass and it becomes 'set' almost permanently!


Maria Katleba MS HT(ASCP)
Pathology Dept. Mgr
Queen of the Valley Medical Center
1000 Trancas Street
Napa CA 94558
(707) 252-4411 x3689 direct
(707) 226-4385 pager
(707) 294-9229 cell- anytime
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com
Sent: Friday, November 19, 2010 8:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nails


We are having a very difficault time with the naisl falling off the slides when we do the PASF stain.  We are using charged slides already and we have tried increasing the baking time during drying to no avail.

Any suggestions?


Roxanne



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Please note that the information contained in this message may be privileged and confidential and protected from disclosure.



From beth.villarreal <@t> novartis.com  Fri Nov 19 12:26:31 2010
From: beth.villarreal <@t> novartis.com (beth.villarreal@novartis.com)
Date: Fri Nov 19 12:26:40 2010
Subject: [Histonet] modified GMS
In-Reply-To: <201011191802.oAJI2oam031996@ch1ssaenov01.novartis.com>
Message-ID: 

We purchase a kit from Sigma, cat #HT-100A-1KT.



Message: 14
Date: Fri, 19 Nov 2010 08:16:35 -0800 (PST)
From: Sheila Haas 
Subject: [Histonet] Modified GMS
To: histonet@lists.utsouthwestern.edu
Message-ID: <24062.7620.qm@web57806.mail.re3.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi all. Years ago, I used a modified GMS procedure that used periodic acid 
in 
place of chromic acid. Would anyone be willing to share their 
procedure for this 
or at least a vendor you might be purchasing a kit from? Thanks in 
advance! 
 
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.

Beth Villarreal, HT(ASCP)
PCS, Discovery Pathology, US
USCA, 611-7104B
Novartis Institutes for BioMedical
Research, Inc.
100 Technology Square
Cambridge, MA 02139
USA
Phone: +1 617 8714725
Email : beth.villarreal@novartis.com
From POWELL_SA <@t> mercer.edu  Fri Nov 19 12:36:49 2010
From: POWELL_SA <@t> mercer.edu (Shirley A. Powell)
Date: Fri Nov 19 12:36:56 2010
Subject: [Histonet] Nails
In-Reply-To: <201011191649.oAJGn4f6018181@spamfilt>
References: <201011191649.oAJGn4f6018181@spamfilt>
Message-ID: <9BF995BC0E47744E9673A41486E24EE22692442E8C@MERCERMAIL.MercerU.local>

So if you can't find Stay On from Surgipath/Leica now, you can make your own additive.  I used to make up an Agar solution, see method below, and used 20 mL of this to my water bath that was about 2000 mL.  

Agar, Agar (Bacto Agar)      2 gm
Distilled water                    800 mL
Dissolve completely in a flask using medium heat and magnetic stirrer.  Cool and add a few grains of thymol.  Store in the frig.  You may need to shake it to break it up before use, but this is what I used for years before the commercial stuff came out.

Shirley

-----Original Message-----
From: Spath, Judith [mailto:spath.judith@marshfieldclinic.org] 
Sent: Friday, November 19, 2010 11:49 AM
To: powell_sa@mercer.edu
Subject: Re: [Histonet] Nails

Right now, there is a serious BACK ORDER on STA ON...just an FYI....

------Original Message------
From:	"Shirley A. Powell" 
Date:	Fri Nov 19, 2010 -- 10:41:30 AM
To:	godsgalnow@aol.com,histonet@lists.utsouthwestern.edu
Subject:	RE: [Histonet] Nails

Switch to Stay On and plain slides, I had problems with charged slides and now have good results with the additive and regular slides.  Cheaper in the long run too.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com
Sent: Friday, November 19, 2010 11:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nails


We are having a very difficault time with the naisl falling off the slides when we do the PASF stain.  We are using charged slides already and we have tried increasing the baking time during drying to no avail.

Any suggestions?


Roxanne



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




______________________________________________________________________
The contents of this message may contain private, protected and/or privileged information.  If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.  Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.  Thank you for your cooperation.

From benjamin.ahlijah <@t> bioreliance.com  Fri Nov 19 15:04:37 2010
From: benjamin.ahlijah <@t> bioreliance.com (Ahlijah, Benjamin)
Date: Fri Nov 19 15:04:58 2010
Subject: [Histonet] Associate Scientist position at BioReliance in Rockville
	MD
Message-ID: 

Great opportunity for Associate Scientist II position in a research lab
in Rockville, MD. (Associate Scientist II---Pathology Services,
Necropsy)

 

 

The Associate Scientist II (AS II) will perform a wide variety of assays
or tests required to characterize product or material safety. They will
make scientific observations, maintain detailed workbooks/documentation
and ensure all documentation fulfills generally accepted
professional/industry standards. They will maintain an understanding of
technological principles and applications of the organization's
services.

 

To see the full job posting and job description, see link below or
contact Nicole Hollingsworth at BioReliance.

 

 

Please apply directly at:   www.bioreliance.com
 

If you are interested in applying for employment with BioReliance and
need special assistance or an accommodation to apply for a posted
position, contact our Human Resources department at 

301-738-1000 or 1-800-756-5658.

 

 

 

 

 

 

 

 

 

 





Regards, 
Ben. 

Benjamin Ahlijah,  BS 
Pathology Lab Supervisor
BioReliance Corporation 
14920 Broschart Road 
Rockville, MD 20850 
Office: 301-610-2602 
Fax:301-610 2199 
benjamin.ahlijah@BioReliance.com 

P please consider the environment before printing this e-mail 

 

BioReliance Corporation, "Celebrating 60 years of contract bioservices" 





This message is intended for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential, and/or exempt from disclosure under applicable law. If the
reader of this message is not the intended recipient, or if you have
reason to believe you are not the intended recipient, you are hereby
notified that any use, dissemination, distribution, or copying of this
communication is strictly prohibited.  If you received this
communication in error, please notify us immediately. Thank you.

 

From Marilyn.A.Weiss <@t> kp.org  Fri Nov 19 18:02:24 2010
From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org)
Date: Fri Nov 19 18:03:09 2010
Subject: [Histonet] I
Message-ID: 


I will be out of the office starting  11/18/2010 and will not return until
11/30/2010.

In my absence please ask for Mary Campbell .  If this is urgent or you need
to speak to me directly  you can contact me on my cell phone number
858-472-4266.
From DianaRip1 <@t> aol.com  Sat Nov 20 05:03:26 2010
From: DianaRip1 <@t> aol.com (DianaRip1@aol.com)
Date: Sat Nov 20 05:03:40 2010
Subject: [Histonet] Nails
Message-ID: <6013f.6317486b.3a1904fe@aol.com>

The least complicated way to cut nails is to face the block, get a small  
shallow dish and put in a few drops of Dawn dish soap and add a little warm  
water.  Just soak for about 10 minutes.  Cut and place on a + slide  and dry 
slide well before staining.
From histotech <@t> imagesbyhopper.com  Sat Nov 20 09:14:45 2010
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Sat Nov 20 09:14:50 2010
Subject: [Histonet] Modified GMS
In-Reply-To: <24062.7620.qm@web57806.mail.re3.yahoo.com>
Message-ID: <78F49141E8B54C4D818AF13800F571CD@hopperPC>

Hi Sheila!

I have sent you a copy (under separate cover) of our GMS procedure using
periodic acid.  Hope it helps!  :o)

Michelle



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Friday, November 19, 2010 11:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Modified GMS


Hi all. Years ago, I used a modified GMS procedure that used?periodic acid
in 
place of chromic acid. Would anyone be willing to share their procedure?for
this 
or at least a vendor you might be purchasing a kit from? Thanks in advance!?
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.


      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

-----
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From jaylundgren <@t> gmail.com  Sat Nov 20 16:09:35 2010
From: jaylundgren <@t> gmail.com (Jay Lundgren)
Date: Sat Nov 20 16:09:39 2010
Subject: [Histonet] prostate orientation
In-Reply-To: <44CDA7F3-1D34-4FB2-B57E-7E0EF0E8045B@yahoo.com>
References: 
	<44CDA7F3-1D34-4FB2-B57E-7E0EF0E8045B@yahoo.com>
Message-ID: 

    Make sure to use embedding sponges, and make sure the person grossing
them teases them straight before putting the top sponge on.  If you gross
them in straight and flat, between sponges, they should stay that way
through processing.  It makes the embedding easier.  Some techs like to use
an embedding weight to help get bx's flat.  I've worked places where we
trimmed the blocks and got 6 levels of prostate bx on 1 slide.

                                                       Jay A. Lundgren M.S,,
HTL (ASCP)
From rsrichmond <@t> gmail.com  Sat Nov 20 22:49:31 2010
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Sat Nov 20 22:49:36 2010
Subject: [Histonet] Re: Modified GMS
Message-ID: 

Sheila Haas at MicroPath Laboratories, Inc. asked about substituting
periodic acid for chromic acid (because CrO3 is a significant hazmat,
I suppose).

We've discussed this on Histonet in previous years, and it's probably
in our archives. Briefly, Freida Carson several years ago showed that
periodic acid under the usual staining conditions is not a reliable
way to identify Histoplasma.

Bob Richmond
Samurai Pathologist
Knoxville TN

From pruegg <@t> ihctech.net  Sun Nov 21 12:17:18 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sun Nov 21 12:18:15 2010
Subject: [Histonet] Nails
In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EB42CE46C@IBMB7Exchange.digestivespecialists.com>
References: <8CD561DD725D8AE-14E0-4503@webmail-d035.sysops.aol.com><9BF995BC0E47744E9673A41486E24EE22692442D9A@MERCERMAIL.MercerU.local>
	<5A2BD13465E061429D6455C8D6B40E390EB42CE46C@IBMB7Exchange.digestivespecialists.com>
Message-ID: <696DC2ED20604F23821E0AEDAB39494E@prueggihctechlt>

I have used elmer's glue coated plain slides for years for tissues difficult
to keep on the slides, such as cartilage, bone, nails, etc.  I make a 5%
solution of the glue in dih20 (about 200 mls) and then load racks (24 slots)
with slides and dip them in the glue solution about 10 times then take the
slides out of the racks and line them up around something to airdry, usually
overnight, you can then store the dried slides next to each other back in
slide boxes for future use.  After they are dry I do not think they have a
shelf life as I have used slides that are more than a year old and they
still work, just the quick dip in the waterbath to pick up the section seems
to activate the glue enough to make the sections stick well.  One cautionary
thing to keep in mind is that the glue itself can be bio reactive with
certain IHC protocols as it may have some ridicule Ig in the glue if it is
made from animals.

Regards,

Patsy   

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Friday, November 19, 2010 9:51 AM
To: 'Shirley A. Powell'; godsgalnow@aol.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Nails

An old old trick is taking a plain slide and coating it with dilute Elmer's
glue and letting it dry. Be quick picking it up from the water bath and let
dry well.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A.
Powell
Sent: Friday, November 19, 2010 11:40 AM
To: godsgalnow@aol.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Nails

Switch to Stay On and plain slides, I had problems with charged slides and
now have good results with the additive and regular slides.  Cheaper in the
long run too.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
godsgalnow@aol.com
Sent: Friday, November 19, 2010 11:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nails


We are having a very difficault time with the naisl falling off the slides
when we do the PASF stain.  We are using charged slides already and we have
tried increasing the baking time during drying to no avail.

Any suggestions?


Roxanne



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From pruegg <@t> ihctech.net  Sun Nov 21 12:43:15 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sun Nov 21 12:43:52 2010
Subject: SPAM-LOW:  [Histonet] Partially muddy slides
In-Reply-To: 
References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB51F9@PHSXMB30.partners.org>
	
Message-ID: 

Sounds to me like an individual tissue collection issue (aka time to
fixative) since it is so random and individual.  Ask those collecting the
samples to record when the sample was removed from the body, and when it
went into fixative if you can get them to do that.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histotech@imagesbyhopper.com
Sent: Tuesday, November 16, 2010 10:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Partially muddy slides

Hi Histonetters!

We are having an issue in our lab with some inconsistent staining.  It's an
odd situation.  One small biopsy can stain reasonably well over most of the
tissue, but then one area of it is muddy.  This does not affect all the
slides in the same staining rack, nor does it affect all the small biopsies
that were processed in the same processing cycle.  Troubleshooting this has
been difficult, at best. It's sort of hit or miss on when/where the
muddy-ness appears.

Here are my thoughts:
** I don't know if this is a processing issue, a staining issue or perhaps
even a collection issue.
** If it affected all small biopsies equally, I would be tempted to question
the processing/fixation.  But it doesn't affect across the board.
**  If all the staining was muddy, I would look to the stainer, but again,
even within the same slide, we can have clear and muddy areas.
**  The stainer is a Leica XL (about 3 years old),  hooked up to tap water
for the washes.
**  We are using a regressive staining method with Fisher brand Protocol
Harris hematoxylin and Protocol Eosin Y.  We use acid alcohol and ammonia
water for differentiating and bluing.
**  The xylene we use is recycled, but verified for purity.
**  The alcohols are recycled, but the last alcohol prior to the
xylene/coverslipping is "pure", not recycled (for just in case).

Suggestions on what could be causing this issue would be greatly
appreciated.

Michelle


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From pruegg <@t> ihctech.net  Sun Nov 21 12:52:11 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sun Nov 21 12:52:49 2010
Subject: SPAM-LOW: [Histonet] glutaraldehyde fixed tissues and genomic DNA
In-Reply-To: <4d3d55b1e538069a91a893b7a2046f7f.squirrel@webmail.ucalgary.ca>
References: <4d3d55b1e538069a91a893b7a2046f7f.squirrel@webmail.ucalgary.ca>
Message-ID: 

Wow this sounds like a molecular biology question that will have to be
figured out and probably no one has done that yet for this particular
situation.  Please write it up if you do figure it out.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
ejschmid@ucalgary.ca
Sent: Monday, November 15, 2010 11:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] glutaraldehyde fixed tissues and genomic DNA

Hello,

I have PFA+glutaraldehyde fixed tissues that I might want to digest down
in order to extract genomic DNA suitable for bisulfite sequencing.

The tissues are embryonic mouse heads. The fixative is a PFA +
glutaraldehyde mix:  3.6 PFA with 5% glut. The glut is grade 1 or electron
miccroscopy grade.

I'd like to pro-K the tissues and then phenol-chloroform extract genomic
DNA.

My hope is that the amount and quality of the DNA will be suitable for
bisulfite conversions for methylation anaylsis. Will the DNA be abundant?
Will it be fairly protein free (does the fixative fix protein to the
DNA?).

Thanks

Eric
University of Calgary
Facutly of Medicine


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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From gloria.cole <@t> usa.net  Sun Nov 21 14:30:29 2010
From: gloria.cole <@t> usa.net (Gloria Cole)
Date: Sun Nov 21 14:30:36 2010
Subject: [Histonet] H pylori control on ventana immuno stainer
Message-ID: <4533D198-CFDC-4EF8-AF09-77C65A1EDF7F@usa.net>

Hi,

I am setting up a new lab and I am using the Ventana immuno stainer, does anyone know of any good HP controls I can purchase for now that will work well with the Ventana?

Thanks,

Gloria
From NSEARCY <@t> swmail.sw.org  Mon Nov 22 08:04:45 2010
From: NSEARCY <@t> swmail.sw.org (Nita Searcy)
Date: Mon Nov 22 08:04:56 2010
Subject: [Histonet] IF Question
Message-ID: <4CEA241C.5D38.00EF.0@swmail.sw.org>

Does freezing your specimen in the cryostat have any effect on the specimen/ result?

I heard that you have to flash freeze ( isopentane)???

Thanks

Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street 
254-724-2438
Temple, Texas, 76502
nsearcy@swmail.sw.org


254-724-2438

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TEL;WORK:4-2438
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EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org
N:Searcy;Nita
TITLE:Manager, Pathology Division
TEL;PAGER:633-2370
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TEL;PAGER:633-2370
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From Melissa.Kuhnla <@t> chsli.org  Mon Nov 22 08:12:43 2010
From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa)
Date: Mon Nov 22 08:12:50 2010
Subject: [Histonet] IHC on cellient
Message-ID: 

Hello,
Is anyone performing IHC on cellient cellblocks?  What did your
validation include?  What are you using as controls?? Thank you.
Melissa


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From relia1 <@t> earthlink.net  Mon Nov 22 09:45:42 2010
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Mon Nov 22 09:45:48 2010
Subject: [Histonet] RELIA Histology Careers Bulletin 11/22/10 And a Few
	Holiday Shopping Tips
Message-ID: 

Hi Histonetters!
 
I hope you are having a great day and gearing up for some holiday fun.
The weather is getting cooler and the Holidays are right around the
corner. 
 
I realize that at this time of year a job change is the furthest thing
from most people?s minds but I have some great opportunities that I
wanted to share along with some great tips for getting some good deals
while holiday shopping.
 
All of my positions are full time permanent positions with premier
companies who offer excellent salaries, benefits and relocation
assistance.  Most of them are willing to look at onsite interviews and
start dates after the holidays so if you or anyone you know might be
looking now or after the first of the year it wouldn?t hurt to shoot me
a quick e-mail at relia1@earthlink.net  or give me a quick call toll
free at 866-607-3542.
Currently I have management positions in CA, NY, AR, LA and CO.  I also
have tech positions in CA, GA, MA, IN, TN, FL, MD and NY.  

Holiday shopping is always fun, crazy, chaotic, inspiring, and
challenging.  Here are some tips that might save you some time, money
and stress.
If you want to get a head start on Black Friday check out the website
www.bfads.net  they have posted the Black Friday Sale ads from your
favorite stores go to the website they have a lot of the ads posted on
their site NOW that will be in your newspaper on Thanksgiving morning.
 
The Monday after Thanksgiving is known as Cyber Monday it is the busiest
online shopping day of the year and there is another website that will
give you heads up on deals for Cyber Monday.  This website is
www.cybermonday.com  The best part of this site is that the stores that
advertise on their site donate a percentage of sales to a scholarship
fund.
 
Again if you or anyone you know is interested in a new opportunity now
or after the holidays get in touch with me.  There are a lot of
recruiters out there but remember I am the only one with the experience,
connections and respect for you and your career that specializes
exclusively in histology.  I want to place you in a new position only if
it is the right place, right time and right position for you.  And I am
always available for career advice, resume assistance or just a chat.
Let me be your career advocate.  
 
Happy Holidays!!!
Pam
866-607-3542
Relia1@earthlink.net  
 
 
 
Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From alisha <@t> ka-recruiting.com  Mon Nov 22 10:06:32 2010
From: alisha <@t> ka-recruiting.com (Alisha Dynan)
Date: Mon Nov 22 10:06:05 2010
Subject: [Histonet] GM of Laboratory Operations Job in NC
Message-ID: <1831796910.1290441992755.JavaMail.cfservice@sl4app3>



Dear Histonet Subscribers,




 


I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm.  I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities?  We are completely free of charge to candidates and and we work on quite a few histotechnician, histotechnologist, and cytotechnologist openings across the country. Our clients typically assist with relocation expenses. 




 

One particular client I am working with is looking for a General Manager of Anatomic Pathology Operations for an anatomic pathology lab in NJ. This laboratory is known for its cutting edge technology, LIS system, and its automation. They are looking for someone with 10-20+ years experience in histology/pathology, preferably an HTL(ASCP) certification, at least 10+ years experience in management/operations, and someone who is a strong leader with excellent communication skills. This is a high level pathology position and offers an excellent compensation and benefits package. If interested, please email me your resume to alisha@ka-recruiting.com.

 

Below is a list of some of the opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates.

 

Current HT and HTL Opportunities: 

 







1. Histology Manager - MI


2. Histology Supervisor - NV


3. Histology Supervisor (must have HTL) - Atlanta, GA


4. Histology Supervisor - Central GA


5. Histotech - NYC, NY


6. Grossing Tech - NYC, NY


7. Histotech - IN


8. Histotech - SC


9. Histotech - MA


10. Pathologist's Assistant - NV


11. Pathologist's Assistant - Maine



 


 














If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is.  

 

If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future.  I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country.  

 


To view some additional opportunities please visit our website at www.ka-recruiting.com.  

 



















Sincerely,

 

Alisha (Taylor) Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

alisha@ka-recruiting.com

www.ka-recruiting.com

 






From shive003 <@t> umn.edu  Mon Nov 22 10:38:27 2010
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Mon Nov 22 10:38:33 2010
Subject: [Histonet] norepinephrine IHC
Message-ID: <29AA898C31C6461F965E5E9A1806854F@auxs.umn.edu>

I'm posting this request for someone who is not a member of Histonet -

Does anyone have a lab that performs norepinephrine IHC testing on species other than human?  If so, do you take outside clients?
  
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003@umn.edu

(Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.)
From JPeters <@t> bostwicklaboratories.com  Mon Nov 22 12:30:58 2010
From: JPeters <@t> bostwicklaboratories.com (Justin Peters)
Date: Mon Nov 22 12:31:04 2010
Subject: [Histonet] Polyoma controls
Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F15271354@mail1.BOSTWICK.COM>

Does anyone know where I can get controls for polyomavirus (SV-40 large
T-antigen)?

 

Justin Peters HTL, QIHC (ASCP)CM

IHC Supervisor

Bostwick Laboratories(tm)
For Absolute Confidence(r)

4355 Innslake Drive
Glen Allen, Virginia 23060
Phone:    (804) 967-9225 ext. 1831
Cell:        (804) 822-6084
Email: jpeters@bostwicklaboratories.com
  

 

From marktarango <@t> gmail.com  Mon Nov 22 12:41:43 2010
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Mon Nov 22 12:41:48 2010
Subject: [Histonet] H pylori control on ventana immuno stainer
In-Reply-To: <4533D198-CFDC-4EF8-AF09-77C65A1EDF7F@usa.net>
References: <4533D198-CFDC-4EF8-AF09-77C65A1EDF7F@usa.net>
Message-ID: 

Hi Gloria,

Are you still looking for some good HP controls?  I could send you a block
of one of our controls if you're intersted.  I have blocks from
several different specimens.  Just let me know your address and fedex
number.

Thanks,

Mark

On Sun, Nov 21, 2010 at 12:30 PM, Gloria Cole  wrote:

> Hi,
>
> I am setting up a new lab and I am using the Ventana immuno stainer, does
> anyone know of any good HP controls I can purchase for now that will work
> well with the Ventana?
>
> Thanks,
>
> Gloria
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From laurie.colbert <@t> huntingtonhospital.com  Mon Nov 22 15:08:59 2010
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Mon Nov 22 15:09:04 2010
Subject: [Histonet] Rusting in Pathology Department
In-Reply-To: <938F8EC5A524D34EB5796E23E52781D34EA013B18D@NADCWPMSGCMS05.hca.corpad.net>
Message-ID: <57BE698966D5C54EAE8612E8941D768309FF69EC@EXCHANGE3.huntingtonhospital.com>

How about decal solution (acid)?  Do you have open containers at the
microtomes that the histotechs use throughout the day?
Straight acids will cause metal to rust.  We have an acid storage
cabinet that is wood, but the hinges and handles are totally rusted out.

Laurie

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Beth.Fye@HCAhealthcare.com
Sent: Thursday, November 18, 2010 5:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rusting in Pathology Department

Here is a question I don't believe I ever seen discussed here before.
Does anyone else have significant rusting issues in their Histology
Departments?  Cabinet hinges, metal outlet plates, printers, etc. are
rusting in our department, especially around the microtome areas.
Please let me know if you are having any of these issues, or can think
of any reason for this.  We had a printer in the lab that the whole
interior rusted out.  This is not minor rusting that I could contribute
to water bath  humidity.


Beth A. Fye, CT (ASCP)
Pathology Technical  Manager
HCA Richmond Hospital Laboratories
office:  (804)228-6564
fax: (804)323-8638





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From micro <@t> superlink.net  Mon Nov 22 15:23:01 2010
From: micro <@t> superlink.net (Markus F. Meyenhofer)
Date: Mon Nov 22 15:23:52 2010
Subject: [Histonet] Rusting in Pathology Department
References: <57BE698966D5C54EAE8612E8941D768309FF69EC@EXCHANGE3.huntingtonhospital.com>
Message-ID: <95E49A4ECF834A5DA7DD40B2EBD0DA43@DJ4VDH31>

It's the fumes of Hydrochloric Acid from solutions.
----- Original Message ----- 
From: "Laurie Colbert" 
To: ; 
Sent: Monday, November 22, 2010 4:08 PM
Subject: RE: [Histonet] Rusting in Pathology Department


How about decal solution (acid)?  Do you have open containers at the
microtomes that the histotechs use throughout the day?
Straight acids will cause metal to rust.  We have an acid storage
cabinet that is wood, but the hinges and handles are totally rusted out.

Laurie

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Beth.Fye@HCAhealthcare.com
Sent: Thursday, November 18, 2010 5:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rusting in Pathology Department

Here is a question I don't believe I ever seen discussed here before.
Does anyone else have significant rusting issues in their Histology
Departments?  Cabinet hinges, metal outlet plates, printers, etc. are
rusting in our department, especially around the microtome areas.
Please let me know if you are having any of these issues, or can think
of any reason for this.  We had a printer in the lab that the whole
interior rusted out.  This is not minor rusting that I could contribute
to water bath  humidity.


Beth A. Fye, CT (ASCP)
Pathology Technical  Manager
HCA Richmond Hospital Laboratories
office:  (804)228-6564
fax: (804)323-8638





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From histologydirector <@t> gmail.com  Tue Nov 23 07:34:29 2010
From: histologydirector <@t> gmail.com (Histology Director)
Date: Tue Nov 23 07:34:36 2010
Subject: [Histonet] GU pathology laboratory looking for histotechs/
 cytotechs in Columbus, Ohio
Message-ID: 

Looking for experienced histology technicians and cytotechnologists in
Columbus, Ohio for new GU pathology laboratory. Excellent compensation with
great benefits. Experience with IHC, FISH, and GU pathology a plus. Send
resume or CV to careers@aksm.com.
From mhale <@t> carisls.com  Tue Nov 23 08:41:01 2010
From: mhale <@t> carisls.com (Hale, Meredith)
Date: Tue Nov 23 08:41:06 2010
Subject: [Histonet] HT Position 
Message-ID: <6F33D8418806044682A391273399860F0610502E@s-irv-ex301.PathologyPartners.intranet>

 

Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time  position
that offers a competitive salary and  the flexible hours allows you to
put your own personal stamp on the laboratory .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mhale@carisls.com

 

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mhale@carisls.com   

 

From sfeher <@t> CMC-NH.ORG  Tue Nov 23 08:50:00 2010
From: sfeher <@t> CMC-NH.ORG (Feher, Stephen)
Date: Tue Nov 23 08:50:03 2010
Subject: [Histonet] ThinPrep vs. SurePath
In-Reply-To: 
References: 
Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC243868BA@exchange.cmc-nh.org>

Hi Jennifer,

I have used both systems, as a cytotech, processing tech, and as a
supervisor.  As a cytotech, there is less area of the slide to have to
review so from that viewpoint it seems more efficient.  The drawback for
me was the size of the cells.  For Gyn specimens, very small high grade
cells can be difficult to detect without some sort of computer assisted
system. The Focal Point system was supposed to provide this assistance
but I found it somewhat unreliable.  This was further complicated by the
Focal Point being about 50% accurate in detecting endocervical cells.
Labs that allowed specimens that automatically signed out cases the
Focal Point deemed at the lowest risk for abnormal cells frequently had
clinicians repeating paps due to there being no endocervical material
detected.  I found that non-gyn specimens processed using SurePath were
very difficult for inexperienced techs and many pathologists simply did
not trust that they were getting the appropriate sampling of cells.
Very experienced cytotechs and some pathologists preferred SurePath for
non-gyns but they are in the minority and I found them difficult and
time consuming to review.  As a processing tech, SurePath was very time
consuming and if a lab has any kind of volume at all, had to have at
least 2 technicians dedicated to nothing but processing them.

ThinPrep has a larger are to look at and those techs who are used to
SurePath do not like to review them because the cells are quite a bit
larger, and if doing manual screening, takes a bit longer to review.
Use of the ThinPrep Imager has cut screening time down considerably.
Time and statistical analysis has shown that the Imager is a very
reliable instrument for indicating those areas of the slide where
abnormal cells may be found.  Criticism has been that the Imager
sometimes misses cells with HPV and viral  effect.  Even so, the primary
focus of many labs is to properly detect and report high grade lesions
and for this the Imager is reliable.  For me, non-gyn specimens were
easier to review and displayed a good distribution of cells.  A good
many pathologists agree and are confident that the cell sampling
adequate.  As a processing tech, ThinPrep processing takes less time and
less personnel than SurePath.  If the T-5000 processor is ever released
by the FDA for use in the US for gyn specimens, this time will be
further reduced.

In the end, the smaller cell distribution size offered by SurePath,
while seemingly more efficient, requires more time to review for many
techs due to the small size of the cells.  ThinPrep currently offers a
better solution to a majority of labs in terms of accuracy, time to
prepare, and Imaging.  

Hope this was helpful.


Steve

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Wednesday, November 10, 2010 6:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ThinPrep vs. SurePath

Does anyone have an opinion as to why there seems to be many more
ThinPreps out there than Sure Path?  The SurePath seems more efficient,
but I might be missing something.
Thank you,
Jennifer MacDonald
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From flnails <@t> texaschildrens.org  Tue Nov 23 10:28:23 2010
From: flnails <@t> texaschildrens.org (Nails, Felton)
Date: Tue Nov 23 10:28:34 2010
Subject: [Histonet] C3D IF
In-Reply-To: <57BE698966D5C54EAE8612E8941D768309E91B7B@EXCHANGE3.huntingtonhospital.com>
References: <201011112244.oABMica0029530@imr-ma03.mx.aol.com>
	<57BE698966D5C54EAE8612E8941D768309E91B7B@EXCHANGE3.huntingtonhospital.com>
Message-ID: 

Is anyone perform immunofluorescence for C3D?

------------------------------------------------------------------------------
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 The information in this e-mail may be confidential and/or
 privileged.  If you are not the intended recipient or an
 authorized representative of the intended recipient, you
 are hereby notified that any review, dissemination, or
 copying of this e-mail and its attachments, if any, or
 the information contained herein is prohibited.  If you
 have received this e-mail in error, please immediately
 notify the sender by return e-mail and delete this e-mail
 from your computer system.  Thank you.
==============================================================================


From Lynne.Bell <@t> cvmc.org  Tue Nov 23 11:09:09 2010
From: Lynne.Bell <@t> cvmc.org (Bell, Lynne)
Date: Tue Nov 23 11:09:14 2010
Subject: [Histonet] HistoGel for cell blocks
Message-ID: 

One of my pathologists is interested in the pros and cons of HistoGel for cell blocks.  I have read the archives and gathered some information for him.  He specifically would like to talk to someone that has been using it for some time.

Along the same line, what method do you find to be the best for cell blocks?

Any help would be appreciated.  Thanks!

Lynne Bell, HT (ASCP)
Histology Team Leader
Central Vermont Medical Center
P. O. Box 547
Barre, VT  05641
802-371-4923


From jaylundgren <@t> gmail.com  Tue Nov 23 12:21:16 2010
From: jaylundgren <@t> gmail.com (Jay Lundgren)
Date: Tue Nov 23 12:21:21 2010
Subject: [Histonet] HistoGel for cell blocks
In-Reply-To: 
References: 
Message-ID: 

    A little bit of plain agar, or a plain TSA slant tube (stolen from
micro), melted in the microwave for <10 seconds, works just as well as
histogel, imho.


                                                                  Jay A.
Lundgren M.S., HTL (ASCP)
From Bonnie.Whitaker <@t> osumc.edu  Tue Nov 23 12:51:15 2010
From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie)
Date: Tue Nov 23 12:51:17 2010
Subject: [Histonet] Histology Lab Manager--Ohio State University Med Center
Message-ID: <6B106EE8C8AAEF449DEA97921DEC116701469D3A9E@EXMBOX-VP05.OSUMC.EDU>

Hi Everyone,
 
We have an opening for the Clinical Histology Laboratory Manager here at The Ohio State University Medical Center (this is my position, as I have accepted another role within our department starting Dec. 1.)
 
We are located in Columbus, OH which is a wonderful city with a population of 1.75 million.  We are the 16th largest city in the US, without the traffic problems  of some of the larger cities.  There are 19 communities within 30 minutes of downtown Columbus.  It really is a lovely area, complete with four seasons.  

The histology lab is a good lab, with a stable, hard-working staff!
 
Here is the official blurb:
 
The OSUMC clinical histology laboratory is a functional laboratory within the Anatomic Pathology branch serving the pathology subspecialty divisions.  The Manager will provide leadership for daily operations of the histology labs, manages human resources, financial and budget planning and preparation, new test development, quality improvement, safety programs, compliance program, successful inspection and accreditation processes, and ensuring current policies and procedures related to technical, operational, and administrative areas of the laboratory.  

For a complete position description and application instructions please go to http://medicalcenter.osu.edu/careers/    and search by posting number 354362. The Ohio State University is an equal opportunity, affirmative active employer. Women, minorities, veterans, and individuals with disabilities are encouraged to apply. 
 

Please don't hesitate to give me a call, or email me if you want more information.
 
Thanks!

Bonnie Whitaker 
Clinical Histology Manager 
The Ohio State University Medical Center 
Anatomic Pathology Branch 
Department of Pathology 
N308B Doan Hall 
410 W. 10th Ave. 
Columbus, OH  43210 

Bonnie.Whitaker@osumc.edu 

phone 614.293.5048 
fax 614.293.7273 
pager 614.346.5013 

 


From sbreeden <@t> nmda.nmsu.edu  Tue Nov 23 12:55:14 2010
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Tue Nov 23 12:55:18 2010
Subject: [Histonet] SOP for Microtomy?
Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4747B@nmdamailsvr.nmda.ad.nmsu.edu>

Polishing up my List of SOPs To Write.  Don't want to bake from scratch
if there's an instant mix (that only applies to writing SOPs, by the
way).  Do you have an SOP for the physical process of microtomy (i.e.,
cutting a paraffin block) that I could plagiarize?  I will trade a
pre-written SOP on Microtomy for a one-way ticket to your closest city
on a glass-bottom bus.  Muchas gracias!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

From Vickroy.Jim <@t> mhsil.com  Tue Nov 23 13:02:24 2010
From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim)
Date: Tue Nov 23 13:02:41 2010
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
Message-ID: <24A4826E8EF0964D86BC5317306F58A55510FE3506@mmc-mail.ad.mhsil.com>

Does anyone know where we can purchase a hydrometer or other instrument for confirming alcohol percentages, such as 70, 85, 95, 100?   We had a mixup in chemicals on a processor and I am going to be asked about instruments to confirm percentages before processing.

Meeting with risk management tomorrow.


James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046


________________________________
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
From alyssa <@t> alliedsearchpartners.com  Tue Nov 23 13:08:02 2010
From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson)
Date: Tue Nov 23 13:08:08 2010
Subject: [Histonet] Histology/IHC Professionals Needed in NY
Message-ID: 

Allied Search Partners has been retained for the following searches. We have
openings in Histology/IHC and for a Histology Lab Aide. Please forward this
along to anyone who you know that would be interested in any of
the following positions. We do offer a referral bonus. Have a wonderful
Thanksgiving Holiday everyone in Histoland!



   1. Please email a copy of updated resume to
   Alyssa@alliedsearchpartners.com for a full job description.
   2. Please send availability for a phone screen with one of our
   recruiters. Once we receive your resume we will contact you to schedule a
   phone screen.
   3. Please indicate which position(s) you are interested in.
   4. Please indicate your salary expectations.

We have the following positions available:



*HISTOLOGY/IHC:*

* *

   1. *Immunohistochemistry Supervisor*

LOCATION: Port Chester, NY area

DEPARTMENT & SCHEDULE:

Day Shift, Monday-Friday (IHC Department)



2.    *Immuniohistochemistry Lead Tech (III)* -*2 positions avaiable*

LOCATION: Port Chester, NY area

SCHEDULE & DEPARTMENT:

10pm-6:30am Monday-Friday (IHC Department)

1pm-9:30pm Monday-Friday

* *

*3.**      Immuniohistochemistry Tech II - 2 positions available*



LOCATION: Port Chester, NY area

SCHEDULE & DEPARTMENT:

1. 7:30pm-4:30am Monday-Friday (IHC Department), Rotating Saturdays

2. 1pm-9:30pm Monday-Friday



*4.**      Histotech I*



LOCATION: Port Chester, NY area

SCHEDULE & DEPARTMENT:

3PM-11:30PM Tuesday-Saturday (Histology)



*5.**      Histology Laboratory Aide*



LOCATION: Port Chester, NY area

SCHEDULE & DEPARTMENT:

Monday-Friday 12pm-8:30pm



*6.**     Histotechnician/Histotechnologist*

* *

LOCATION: Clifton, NJ area

SCHEDULE & DEPARTMENT:

Day Hours, Full Time (Histology)


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LinkedIN:http://www.linkedin.com/in/alyssapetersonasp

Allied Search Partners

T: 888.388.7571

F: 888.388.7572

www.alliedsearchpartners.com

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From brett_connolly <@t> merck.com  Tue Nov 23 13:50:48 2010
From: brett_connolly <@t> merck.com (Connolly, Brett M)
Date: Tue Nov 23 13:50:51 2010
Subject: [Histonet] CD20 for IHC on  mouse FFPE tissue
Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D752BF46@usctmx1176.merck.com>

Can anyone recommend a good, clean antibody please, preferably from
rabbit host?

Thanks in advance,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
From DKBoyd <@t> chs.net  Tue Nov 23 14:47:38 2010
From: DKBoyd <@t> chs.net (DKBoyd@chs.net)
Date: Tue Nov 23 14:47:48 2010
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55510FE3506@mmc-mail.ad.mhsil.com>
Message-ID: 

Fisher Scientific.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd@chs.net







"Vickroy, Jim"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
11/23/2010 02:07 PM

To
"histonet@lists.utsouthwestern.edu" 
cc

Subject
[Histonet] CHECKING ALCOHOL CONCENTRATIONS






Does anyone know where we can purchase a hydrometer or other instrument 
for confirming alcohol percentages, such as 70, 85, 95, 100?   We had a 
mixup in chemicals on a processor and I am going to be asked about 
instruments to confirm percentages before processing.

Meeting with risk management tomorrow.


James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046


________________________________
This message (including any attachments) contains confidential information 
intended for a specific individual and purpose, and is protected by law. 
If you are not the intended recipient, you should delete this message. Any 
disclosure, copying, or distribution of this message, or the taking of any 
action based on it, is strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Disclaimer: This electronic message may contain information that is
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is intended only for the use of the individual(s) and entity named
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From thomas.crowell <@t> novartis.com  Tue Nov 23 15:35:27 2010
From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com)
Date: Tue Nov 23 15:35:52 2010
Subject: [Histonet] Thomas Crowell is out of the office.
Message-ID: 


I will be out of the office starting  11/23/2010 and will not return until
11/29/2010.

Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.
From sgoebel <@t> mirnarx.com  Tue Nov 23 15:48:50 2010
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Tue Nov 23 15:48:57 2010
Subject: [Histonet] 70% alcohol
Message-ID: 

Hello all!!

I moved jobs, but am still here in 80 degree Austin weather...

Question.  If tissue has been stored in 70% alcohol for up to a year,
does there need to be anything done to it before processing?  I am
assuming that some rehydration might be necessary?  If not can you just
throw it on  the processor on a normal cycle?  Thanks for you help!!

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

From Sandra.Harrison3 <@t> va.gov  Tue Nov 23 15:50:26 2010
From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.)
Date: Tue Nov 23 15:50:33 2010
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
In-Reply-To: 
References: <24A4826E8EF0964D86BC5317306F58A55510FE3506@mmc-mail.ad.mhsil.com>
	
Message-ID: 

CBG Biotech.  Their contact info is 1-800-941-9484 or you can e mail
them at info@cbgbiotech.com.  I purchased my recycler through them and
have ordered extra hydrometers from them, too.  They give excellent
customer service.

Sandy Harrison, HTL(ASCP)
Histology Supervisor, VA Minneapolis
612-467-2449

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
DKBoyd@chs.net
Sent: Tuesday, November 23, 2010 2:48 PM
To: Vickroy, Jim
Cc: histonet@lists.utsouthwestern.edu;
histonet-bounces@lists.utsouthwestern.edu
Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS

Fisher Scientific.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional
Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l
F: 
804-765-5582 l dkboyd@chs.net







"Vickroy, Jim"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
11/23/2010 02:07 PM

To
"histonet@lists.utsouthwestern.edu" 
cc

Subject
[Histonet] CHECKING ALCOHOL CONCENTRATIONS






Does anyone know where we can purchase a hydrometer or other instrument 
for confirming alcohol percentages, such as 70, 85, 95, 100?   We had a 
mixup in chemicals on a processor and I am going to be asked about 
instruments to confirm percentages before processing.

Meeting with risk management tomorrow.


James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046


________________________________
This message (including any attachments) contains confidential
information 
intended for a specific individual and purpose, and is protected by law.

If you are not the intended recipient, you should delete this message.
Any 
disclosure, copying, or distribution of this message, or the taking of
any 
action based on it, is strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------------------------------------------------
--
Disclaimer: This electronic message may contain information that is
Proprietary, Confidential, or legally privileged or protected. It
is intended only for the use of the individual(s) and entity named
in the message. If you are not an intended recipient of this
message, please notify the sender immediately and delete the
material from your computer. Do not deliver, distribute or copy
this message and do not disclose its contents or take any action in
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_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From rjbuesa <@t> yahoo.com  Tue Nov 23 15:54:29 2010
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Nov 23 15:54:33 2010
Subject: [Histonet] 70% alcohol
In-Reply-To: 
Message-ID: <495355.41385.qm@web65714.mail.ac4.yahoo.com>

It is always better to be safe than sorry. You should start in EtOL95% ? 100% and clear as usual before infiltration.
Ren? J.

--- On Tue, 11/23/10, sgoebel@mirnarx.com  wrote:


From: sgoebel@mirnarx.com 
Subject: [Histonet] 70% alcohol
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, November 23, 2010, 4:48 PM


Hello all!!

I moved jobs, but am still here in 80 degree Austin weather...

Question.? If tissue has been stored in 70% alcohol for up to a year,
does there need to be anything done to it before processing?? I am
assuming that some rehydration might be necessary?? If not can you just
throw it on? the processor on a normal cycle?? Thanks for you help!!



Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas? 78744

(512)901-0900 ext. 6912



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From Laura.Miller <@t> leica-microsystems.com  Tue Nov 23 16:02:57 2010
From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com)
Date: Tue Nov 23 16:03:09 2010
Subject: [Histonet] Laura Miller is Out of  the Office.
Message-ID: 


I will be out of the office starting  11/23/2010 and will not return until
11/29/2010.

I will be on vacation until Monday, Novemebr 29th.  I will respond to your
message when I return back to the office.  Happy Thanksgiving!


______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________

From cforster <@t> umn.edu  Tue Nov 23 16:09:54 2010
From: cforster <@t> umn.edu (Colleen Forster)
Date: Tue Nov 23 16:09:51 2010
Subject: [Histonet] Cyclin D2 staining
Message-ID: <4CEC3BB2.1020404@umn.edu>

  Has anyone out in histo and been able to get good Cyclin D2 staining 
in FFPE samples? If so, please share.

Thanks,

Colleen Forster
U of MN
From algranth <@t> email.arizona.edu  Tue Nov 23 17:44:20 2010
From: algranth <@t> email.arizona.edu (Andrea Grantham)
Date: Tue Nov 23 17:44:25 2010
Subject: [Histonet] Thanksgiving
Message-ID: <3F1267A8-2E41-410E-80E9-E52048E3DF17@email.arizona.edu>

I'm off for the holiday. Happy Thanksgiving to all!
Even in these challenging days we can still count our blessings.

Andi



Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth@email.arizona.edu
Tel: 520.626.4415     Fax: 520.626.2097

"happy slicing and dicing and may all your stains work perfectly" -  
Paula Sicurello
P Please consider the environment before printing this email.




From lpwenk <@t> sbcglobal.net  Tue Nov 23 19:09:45 2010
From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk)
Date: Tue Nov 23 19:09:48 2010
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55510FE3506@mmc-mail.ad.mhsil.com>
References: <24A4826E8EF0964D86BC5317306F58A55510FE3506@mmc-mail.ad.mhsil.com>
Message-ID: <9687A90389ED43D19488C1B673E84409@HP2010>

Lab Safety Supply   www.lss.com
Cole-Parmer   www.coleparmer.com
Fisher Scientific  www.fishersci.com

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

--------------------------------------------------
From: "Vickroy, Jim" 
Sent: Tuesday, November 23, 2010 2:02 PM
To: 
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS

> Does anyone know where we can purchase a hydrometer or other instrument 
> for confirming alcohol percentages, such as 70, 85, 95, 100?   We had a 
> mixup in chemicals on a processor and I am going to be asked about 
> instruments to confirm percentages before processing.
>
> Meeting with risk management tomorrow.
>
>
> James Vickroy BS, HT(ASCP)
>
> Surgical  and Autopsy Pathology Technical Supervisor
> Memorial Medical Center
> 217-788-4046
>
>
> ________________________________
> This message (including any attachments) contains confidential information 
> intended for a specific individual and purpose, and is protected by law. 
> If you are not the intended recipient, you should delete this message. Any 
> disclosure, copying, or distribution of this message, or the taking of any 
> action based on it, is strictly prohibited.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From BMolinari <@t> heart.thi.tmc.edu  Wed Nov 24 06:16:55 2010
From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy)
Date: Wed Nov 24 06:16:59 2010
Subject: [Histonet] 70% alcohol
In-Reply-To: <495355.41385.qm@web65714.mail.ac4.yahoo.com>
References: 
	<495355.41385.qm@web65714.mail.ac4.yahoo.com>
Message-ID: 

That is how I handle ours as well. Start in 95% and proceed with the normal processing protocol.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston,TX 77030
832-355-6524	
832-355-6812 (fax)

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, November 23, 2010 3:54 PM
To: histonet@lists.utsouthwestern.edu; sgoebel@mirnarx.com
Subject: Re: [Histonet] 70% alcohol

It is always better to be safe than sorry. You should start in EtOL95% ? 100% and clear as usual before infiltration.
Ren? J.

--- On Tue, 11/23/10, sgoebel@mirnarx.com  wrote:


From: sgoebel@mirnarx.com 
Subject: [Histonet] 70% alcohol
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, November 23, 2010, 4:48 PM


Hello all!!

I moved jobs, but am still here in 80 degree Austin weather...

Question.? If tissue has been stored in 70% alcohol for up to a year,
does there need to be anything done to it before processing?? I am
assuming that some rehydration might be necessary?? If not can you just
throw it on? the processor on a normal cycle?? Thanks for you help!!



Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas? 78744

(512)901-0900 ext. 6912



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From litepath2000 <@t> yahoo.com  Wed Nov 24 07:56:42 2010
From: litepath2000 <@t> yahoo.com (NYSHisto)
Date: Wed Nov 24 07:56:48 2010
Subject: [Histonet] Looking for...
Message-ID: <600329.24994.qm@web120710.mail.ne1.yahoo.com>

Tim Morkin
Could you please contact me off line?
Thanks

 -------
Luis Chiriboga Ph.D.
Assistant Professor of Pathology
President, New York State Histotechnological Society
NYSHS Website: www.nyhisto.org
NYSHS Message Board: http://tech.groups.yahoo.com/group/NYSHS1972/


This email message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain information that is proprietary, 
confidential, and exempt from disclosure under applicable law. Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you have received 
this email in error please notify the sender by return email and delete the 
original message. Please note, the recipient should check this email and any 
attachments for the presence of viruses. The organization accepts no liability 
for any damage caused by any virus transmitted by this email.



      
From mhale <@t> carisls.com  Wed Nov 24 09:26:49 2010
From: mhale <@t> carisls.com (Hale, Meredith)
Date: Wed Nov 24 09:26:55 2010
Subject: [Histonet] HT Position 
Message-ID: <6F33D8418806044682A391273399860F0616CB46@s-irv-ex301.PathologyPartners.intranet>

Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time  position
that offers a competitive salary and  the flexible hours allows you to
put your own personal stamp on the laboratory .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mahle@carisls.com 

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mhale@carisls.com   

 

From mhale <@t> carisls.com  Wed Nov 24 09:27:04 2010
From: mhale <@t> carisls.com (Hale, Meredith)
Date: Wed Nov 24 09:27:06 2010
Subject: [Histonet] Recall: HT Position 
Message-ID: <6F33D8418806044682A391273399860F0616CB47@s-irv-ex301.PathologyPartners.intranet>

Hale, Meredith would like to recall the message, "HT Position ".
From mhale <@t> carisls.com  Wed Nov 24 09:28:29 2010
From: mhale <@t> carisls.com (Hale, Meredith)
Date: Wed Nov 24 09:28:34 2010
Subject: [Histonet] HT Position 
Message-ID: <6F33D8418806044682A391273399860F0616CB51@s-irv-ex301.PathologyPartners.intranet>

Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time  position
that offers a competitive salary and  the flexible hours allows you to
put your own personal stamp on the laboratory .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mhale@carisls.com

 

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mhale@carisls.com   

 

From sbreeden <@t> nmda.nmsu.edu  Wed Nov 24 10:38:19 2010
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Wed Nov 24 10:38:24 2010
Subject: [Histonet] Microtomy SOP
Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47489@nmdamailsvr.nmda.ad.nmsu.edu>

Thanks to everyone (seven of you!) that sent me examples of your
microtomy SOP.  I'll pick and choose from all to build my own - and it
will save a lot of time, I'm positive.  I tend to make my SOPs rather
"generic" as far as specific steps, equipment names, etc., at the behest
of my QA manager.  We'd rather not be too specific in case momentary
deviation is required - that's a fine line, let me tell you!

 

Thanks to my Faithful Contributors and have a wonderful Thanksgiving
holiday.

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

From heather.mcleod <@t> webmail.co.za  Wed Nov 24 11:42:51 2010
From: heather.mcleod <@t> webmail.co.za (heather.mcleod@webmail.co.za)
Date: Wed Nov 24 11:42:57 2010
Subject: [Histonet] carnoys fluid
Message-ID: 

Dear Histonetters,

We do IHC on pap stained cyto smears.  The results do not always "behave"
accordingly on the smears that have been exposed to Carnoys. Does anybody know
whether Carnoys Fluid affects IHC on cytology smears?  I have seen papers that
suggest improved IHC after Carnoys but maybe Histonetters feel difefrently?
Thanks 
Heather

____________________________________________________________
South Africas premier free email service - www.webmail.co.za 

Free SMSs  everyday!  Gratis, Forniet! http://clients.wm.co.za/20030688/



From sgoebel <@t> mirnarx.com  Wed Nov 24 12:37:04 2010
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Wed Nov 24 12:37:10 2010
Subject: [Histonet] Histology Consultant
Message-ID: 

What is the going rate for a working histology consultant in Texas?  For
instance go in and process, embed, cut, and stain slides when needed?

Thanks

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

From pablo.sanchez <@t> usc.es  Wed Nov 24 13:09:13 2010
From: pablo.sanchez <@t> usc.es (Pablo Sanchez-Quinteiro)
Date: Wed Nov 24 13:09:13 2010
Subject: [Histonet] Help cutting horse tendon.
Message-ID: <923n2i$3mh5o@correo1bi.usc.es>

Dear listers,

I am trying to cut whole sections of the tendon of the digital flexor 
muscle of the horse. The piece is around 1 mm long and very elastic 
and fibrous. I have tried formalin and Bouin fixation, the latest 
better. With paraffin embedding the fibrous elements of the tendon 
spoil the sections. In freezing microtome the sections seem nice but 
the interfascicullar spaces are too wide at the microscope.

I would very grateful for any hint, suggestion, etc.

Best regards,

Pablo


From liz <@t> premierlab.com  Wed Nov 24 13:14:11 2010
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Wed Nov 24 13:11:01 2010
Subject: [Histonet] evans blue
Message-ID: 

Hello everyone and Happy Thanksgiving

 

Does anyone out there know if I inject evens blue dye in tissue will it
survive 10% NBF fixation and then processing to paraffin block.  Its
supposed to be taken up by cells that are undergoing lysis  Basically I
need to be able to detect cell lysis on paraffin sections.

 

Thanks in advance

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

From sbreeden <@t> nmda.nmsu.edu  Wed Nov 24 13:19:43 2010
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Wed Nov 24 13:19:47 2010
Subject: [Histonet] Microtomy SOP
Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4748E@nmdamailsvr.nmda.ad.nmsu.edu>

I am sorry to say that as I received the SOPs on Microtomy, I printed
and deleted them.  I apologize.

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

From plucas <@t> biopath.org  Wed Nov 24 13:29:20 2010
From: plucas <@t> biopath.org (Paula Lucas)
Date: Wed Nov 24 13:22:25 2010
Subject: [Histonet] Cryostat Question Please
Message-ID: 

Hello all
 
Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat?  If so,
what is your opinion/pros and cons?
 
Thanks so much in advance,
Paula 
Lab Manager
BP Medical Group
From billodonnell <@t> catholichealth.net  Wed Nov 24 15:14:15 2010
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Wed Nov 24 15:14:37 2010
Subject: [Histonet] Cryostat Question Please
In-Reply-To: 
References: 
Message-ID: 

Very consistant, easy to use. 

2 drawbacks  

1. We got the decontamination add-on trying to automate the CAP
requirement, but now it seems Thermo says it is not an adequate process.
I believe they are doing more intense studies, but for the time being, I
have to decontaminate it by hand. 

2. OCT and tissue scraps build up under collection device and cause the
mechanism to "clunk" unless buildup is removed. 

These two things add a good chunk of time to my day.

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Wednesday, November 24, 2010 1:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Question Please

Hello all
 
Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat?  If so,
what is your opinion/pros and cons?
 
Thanks so much in advance,
Paula
Lab Manager
BP Medical Group
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From jaylundgren <@t> gmail.com  Wed Nov 24 16:29:38 2010
From: jaylundgren <@t> gmail.com (Jay Lundgren)
Date: Wed Nov 24 16:29:43 2010
Subject: [Histonet] Histology Consultant
In-Reply-To: 
References: 
Message-ID: 

     If you have to ask, you can't afford it.

                                                 Jay A. Lundgren M.S., HTL
(ASCP)
From histopathy <@t> hotmail.com  Wed Nov 24 19:16:39 2010
From: histopathy <@t> hotmail.com (histopathy@hotmail.com)
Date: Wed Nov 24 19:16:41 2010
Subject: [Histonet] Crystalization of Schiff's Reagent
Message-ID: <632810348-1290647796-cardhu_decombobulator_blackberry.rim.net-2002632049-@bda811.bisx.prod.on.blackberry>

Hello Histonetters,

Has anyone had issues with your Schiff's reagent crystallizing?  I cannot remember the cause when it happened one time several years ago.  Any ideas?  Our refrigerator is the appropriate temperature and the Schiff's reagent is brand new...happened to previously ordered bottles, also.

Pam
Sent on the Sprint? Now Network from my BlackBerry?
From irena.kirbis <@t> hotmail.com  Thu Nov 25 01:40:33 2010
From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS)
Date: Thu Nov 25 01:40:40 2010
Subject: [Histonet] carnoys fluid
In-Reply-To: 
References: 
Message-ID: 


I would recommend methanol or ethanol for fixation, smears itself can be culprit of inconsistent staining
Irena
> To: histonet@lists.utsouthwestern.edu
> From: heather.mcleod@webmail.co.za
> Date: Wed, 24 Nov 2010 19:42:51 +0200
> Subject: [Histonet] carnoys fluid
> 
> Dear Histonetters,
> 
> We do IHC on pap stained cyto smears. The results do not always "behave"
> accordingly on the smears that have been exposed to Carnoys. Does anybody know
> whether Carnoys Fluid affects IHC on cytology smears? I have seen papers that
> suggest improved IHC after Carnoys but maybe Histonetters feel difefrently?
> Thanks 
> Heather
> 
> ____________________________________________________________
> South Africas premier free email service - www.webmail.co.za 
> 
> Free SMSs everyday! Gratis, Forniet! http://clients.wm.co.za/20030688/
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From bakevictoria <@t> gmail.com  Thu Nov 25 04:53:03 2010
From: bakevictoria <@t> gmail.com (Victoria Baker)
Date: Thu Nov 25 04:53:08 2010
Subject: [Histonet] Histology Consultant
In-Reply-To: 
References: 
	
Message-ID: 

What he said!  However, what you describe is more like 'contract' work which
the going rate varies.  Vikki

On Wed, Nov 24, 2010 at 5:29 PM, Jay Lundgren  wrote:

>     If you have to ask, you can't afford it.
>
>                                                 Jay A. Lundgren M.S., HTL
> (ASCP)
>  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From amosbrooks <@t> gmail.com  Thu Nov 25 05:14:55 2010
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Thu Nov 25 10:15:37 2010
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
In-Reply-To: <4ced52d7.ad05ec0a.583f.640fSMTPIN_ADDED@mx.google.com>
References: <4ced52d7.ad05ec0a.583f.640fSMTPIN_ADDED@mx.google.com>
Message-ID: <201011250614.56234.amosbrooks@gmail.com>

Hi Jim,
     Hydrometers can get really expensive. I searched around for one with a 
good price and stumbled on this one from Cole Parmer (now Thermo like 
everyone else in the world):
Thermo Scientific ERTCO? Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200 Proof, 
Plain Form ... CAT# EW-08285-00
	I picked it up for $29.50, but that was with my University discount. I'm not 
sure what regular price is or what discounts you might be able to get. It sure 
beat the heck out of some of these $200 ones out there. This one has both ETOH 
percentages as well as ETOH proofs. It works well for us.

Happy Thanksgiving,
Amos


Message: 4
Date: Tue, 23 Nov 2010 13:02:24 -0600
From: "Vickroy, Jim" 
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
To: "histonet@lists.utsouthwestern.edu"
????????
Message-ID:
????????<24A4826E8EF0964D86BC5317306F58A55510FE3506@mmc-
mail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"

Does anyone know where we can purchase a hydrometer or other instrument for 
confirming alcohol percentages, such as 70, 85, 95, 100? ? We had a mixup in 
chemicals on a processor and I am going to be asked about instruments to 
confirm percentages before processing.

Meeting with risk management tomorrow.


James Vickroy BS, HT(ASCP)

Surgical ?and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046

From amosbrooks <@t> gmail.com  Thu Nov 25 05:31:27 2010
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Thu Nov 25 10:31:49 2010
Subject: [Histonet] CD20 for IHC on  mouse FFPE tissue
Message-ID: <201011250631.27710.amosbrooks@gmail.com>

Hi Brett,
	CD20 is not going to work for mouse tissue. You need CD45R (clone B220). This 
will selectively label B cells just like CD20 in humans. I get this from BD 
Biosciences (Now Thermo ... again) Catalog # 347460. It is raised in Rats and 
works great as long as you have a good rat secondary. The one from Jackson is 
good.

Happy Thanksgiving,
Amos

From me <@t> ccalexander.com  Thu Nov 25 12:15:39 2010
From: me <@t> ccalexander.com (Alex Cummins)
Date: Thu Nov 25 12:15:44 2010
Subject: [Histonet] evans blue
Message-ID: <004e01cb8ccc$c4732a90$4d597fb0$@com>

It has been a while but I know it will survive NBF but if my memory is
correct it will be destroyed in paraffin embedding. It is not xylene
tolerant.

Alex

From me <@t> ccalexander.com  Thu Nov 25 12:20:04 2010
From: me <@t> ccalexander.com (Alex Cummins)
Date: Thu Nov 25 12:20:10 2010
Subject: [Histonet] Help cutting horse tendon
Message-ID: <005301cb8ccd$6253e240$26fba6c0$@com>

I am new to the list so I don't know your experience. If your spaces are too
wide did you cryoprotect before freezing? As freezing when done right gives
very consistent size retention. 

Alex

From me <@t> ccalexander.com  Thu Nov 25 12:52:05 2010
From: me <@t> ccalexander.com (Alex Cummins)
Date: Thu Nov 25 12:52:09 2010
Subject: [Histonet] Evans blue
Message-ID: <006c01cb8cd1$dbad6ae0$930840a0$@com>

OK I was wrong. Curiosity got me to go the Conn's Biological stain and it
clearly states that is insoluble in Xylene. And it is barely soluble in
water or Alcohol.  So in theory it would survive paraffin embedding. Fabrice
informed me that it will survive paraffin embedding  but not
deparaffinizing. I am very confused by this for me embedding uses the same
chemistry as deparaffinizing. And as to coverslip you only need to
deparaffinize to Xylene. Counterstaining could be an issue. When I used
Evans blue in the past we only used frozen sections and fluorescent mounting
media to cover slip. Joined today and already learning

Alex

From llewllew <@t> shaw.ca  Thu Nov 25 14:11:07 2010
From: llewllew <@t> shaw.ca (Bryan Llewellyn)
Date: Thu Nov 25 14:11:27 2010
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
In-Reply-To: <201011250614.56234.amosbrooks@gmail.com>
References: <4ced52d7.ad05ec0a.583f.640fSMTPIN_ADDED@mx.google.com>
	<201011250614.56234.amosbrooks@gmail.com>
Message-ID: 

Specific gravity is mass/volume.  In this context that is grams/millilitre. 
It can easily be measured without a hydrometer.

1.  Obtain a 10 ml beaker and weigh it to 2 decimal places.
2.  Measure 10 mL of the alcohol with a volumetric pipette and place in the 
beaker.
3.  Reweigh the beaker with the alcohol in it, again to 2 decimal places.
4.  Subtract the weight of the beaker from the weight of the beaker and 
alcohol, giving the weight of the alcohol
5.  Divide the weight of the alcohol by 10 to get the SG to 3 decimal 
places.

Bryan Llewellyn


----- Original Message ----- 
From: "Amos Brooks" 
To: ; 
Sent: Thursday, November 25, 2010 3:14 AM
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS


Hi Jim,
     Hydrometers can get really expensive. I searched around for one with a
good price and stumbled on this one from Cole Parmer (now Thermo like
everyone else in the world):
Thermo Scientific ERTCO? Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200 
Proof,
Plain Form ... CAT# EW-08285-00
I picked it up for $29.50, but that was with my University discount. I'm not
sure what regular price is or what discounts you might be able to get. It 
sure
beat the heck out of some of these $200 ones out there. This one has both 
ETOH
percentages as well as ETOH proofs. It works well for us.

Happy Thanksgiving,
Amos


Message: 4
Date: Tue, 23 Nov 2010 13:02:24 -0600
From: "Vickroy, Jim" 
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
To: "histonet@lists.utsouthwestern.edu"

Message-ID:
<24A4826E8EF0964D86BC5317306F58A55510FE3506@mmc-
mail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"

Does anyone know where we can purchase a hydrometer or other instrument for
confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in
chemicals on a processor and I am going to be asked about instruments to
confirm percentages before processing.

Meeting with risk management tomorrow.


James Vickroy BS, HT(ASCP)

Surgical and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From njblademaster <@t> gmail.com  Thu Nov 25 18:51:25 2010
From: njblademaster <@t> gmail.com (Nathan Jentsch)
Date: Thu Nov 25 18:51:49 2010
Subject: [Histonet] Re: Histology Consultant
Message-ID: 

If you don't know what the going rate is, that is exactly why you ask.  That
is what this forum is for.  A little professionalism is appreciated.

Nathan Jentsch
BS HT(ASCP)
From marilyngamble <@t> comcast.net  Thu Nov 25 22:04:01 2010
From: marilyngamble <@t> comcast.net (marilyngamble@comcast.net)
Date: Thu Nov 25 22:04:07 2010
Subject: [Histonet] Re: Histonet Digest, Vol 84, Issue 28
Message-ID: <2051139693.1525047.1290744241470.JavaMail.root@sz0135a.westchester.pa.mail.comcast.net>


----- Original Message ----- 
From: histonet-request@lists.utsouthwestern.edu 
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 84, Issue 28 

Send Histonet mailing list submissions to 
????????histonet@lists.utsouthwestern.edu 

To subscribe or unsubscribe via the World Wide Web, visit 
????????http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
or, via email, send a message with subject or body 'help' to 
????????histonet-request@lists.utsouthwestern.edu 

You can reach the person managing the list at 
????????histonet-owner@lists.utsouthwestern.edu 

When replying, please edit your Subject line so it is more specific 
than "Re: Contents of Histonet digest..." 


Today's Topics: 

?? 1. Polyoma controls (Justin Peters) 
?? 2. Re: H pylori control on ventana immuno stainer (Mark Tarango) 
?? 3. RE: Rusting in Pathology Department (Laurie Colbert) 
?? 4. Re: Rusting in Pathology Department (Markus F. Meyenhofer) 
?? 5. GU pathology laboratory looking for histotechs/ cytotechs in 
?? ? ?Columbus, Ohio (Histology Director) 
?? 6. HT Position ?(Hale, Meredith) 
?? 7. RE: ThinPrep vs. SurePath (Feher, Stephen) 
?? 8. C3D IF (Nails, Felton) 
?? 9. HistoGel for cell blocks (Bell, Lynne) 


---------------------------------------------------------------------- 

Message: 1 
Date: Mon, 22 Nov 2010 13:30:58 -0500 
From: "Justin Peters"  
Subject: [Histonet] Polyoma controls 
To:  
Message-ID: 
????????<24D22DE9E488AA43BF92A4389F2DDB1F15271354@mail1.BOSTWICK.COM> 
Content-Type: text/plain;????????charset="us-ascii" 

Does anyone know where I can get controls for polyomavirus (SV-40 large 
T-antigen)? 

? 

Justin Peters HTL, QIHC (ASCP)CM 

IHC Supervisor 

Bostwick Laboratories(tm) 
For Absolute Confidence(r) 

4355 Innslake Drive 
Glen Allen, Virginia 23060 
Phone: ? ?(804) 967-9225 ext. 1831 
Cell: ? ? ? ?(804) 822-6084 
Email: jpeters@bostwicklaboratories.com 
 ? 

? 



------------------------------ 

Message: 2 
Date: Mon, 22 Nov 2010 10:41:43 -0800 
From: Mark Tarango  
Subject: Re: [Histonet] H pylori control on ventana immuno stainer 
To: Gloria Cole  
Cc: histonet@lists.utsouthwestern.edu 
Message-ID: 
???????? 
Content-Type: text/plain; charset=ISO-8859-1 

Hi Gloria, 

Are you still looking for some good HP controls? ?I could send you a block 
of one of our controls if you're intersted. ?I have blocks from 
several different specimens. ?Just let me know your address and fedex 
number. 

Thanks, 

Mark 

On Sun, Nov 21, 2010 at 12:30 PM, Gloria Cole  wrote: 

> Hi, 
> 
> I am setting up a new lab and I am using the Ventana immuno stainer, does 
> anyone know of any good HP controls I can purchase for now that will work 
> well with the Ventana? 
> 
> Thanks, 
> 
> Gloria 
> _______________________________________________ 
> Histonet mailing list 
> Histonet@lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
> 


------------------------------ 

Message: 3 
Date: Mon, 22 Nov 2010 13:08:59 -0800 
From: "Laurie Colbert"  
Subject: RE: [Histonet] Rusting in Pathology Department 
To: ,???????? 
Message-ID: 
????????<57BE698966D5C54EAE8612E8941D768309FF69EC@EXCHANGE3.huntingtonhospital.com> 
???????? 
Content-Type: text/plain;????????charset="us-ascii" 

How about decal solution (acid)? ?Do you have open containers at the 
microtomes that the histotechs use throughout the day? 
Straight acids will cause metal to rust. ?We have an acid storage 
cabinet that is wood, but the hinges and handles are totally rusted out. 

Laurie 

-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of 
Beth.Fye@HCAhealthcare.com 
Sent: Thursday, November 18, 2010 5:10 PM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Rusting in Pathology Department 

Here is a question I don't believe I ever seen discussed here before. 
Does anyone else have significant rusting issues in their Histology 
Departments? ?Cabinet hinges, metal outlet plates, printers, etc. are 
rusting in our department, especially around the microtome areas. 
Please let me know if you are having any of these issues, or can think 
of any reason for this. ?We had a printer in the lab that the whole 
interior rusted out. ?This is not minor rusting that I could contribute 
to water bath ?humidity. 


Beth A. Fye, CT (ASCP) 
Pathology Technical ?Manager 
HCA Richmond Hospital Laboratories 
office: ?(804)228-6564 
fax: (804)323-8638 
 




_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



------------------------------ 

Message: 4 
Date: Mon, 22 Nov 2010 16:23:01 -0500 
From: "Markus F. Meyenhofer"  
Subject: Re: [Histonet] Rusting in Pathology Department 
To: "Laurie Colbert" , 
????????,  
Message-ID: <95E49A4ECF834A5DA7DD40B2EBD0DA43@DJ4VDH31> 
Content-Type: text/plain; format=flowed; charset="iso-8859-1"; 
????????reply-type=original 

It's the fumes of Hydrochloric Acid from solutions. 
----- Original Message ----- 
From: "Laurie Colbert"  
To: ;  
Sent: Monday, November 22, 2010 4:08 PM 
Subject: RE: [Histonet] Rusting in Pathology Department 


How about decal solution (acid)? ?Do you have open containers at the 
microtomes that the histotechs use throughout the day? 
Straight acids will cause metal to rust. ?We have an acid storage 
cabinet that is wood, but the hinges and handles are totally rusted out. 

Laurie 

-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of 
Beth.Fye@HCAhealthcare.com 
Sent: Thursday, November 18, 2010 5:10 PM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Rusting in Pathology Department 

Here is a question I don't believe I ever seen discussed here before. 
Does anyone else have significant rusting issues in their Histology 
Departments? ?Cabinet hinges, metal outlet plates, printers, etc. are 
rusting in our department, especially around the microtome areas. 
Please let me know if you are having any of these issues, or can think 
of any reason for this. ?We had a printer in the lab that the whole 
interior rusted out. ?This is not minor rusting that I could contribute 
to water bath ?humidity. 


Beth A. Fye, CT (ASCP) 
Pathology Technical ?Manager 
HCA Richmond Hospital Laboratories 
office: ?(804)228-6564 
fax: (804)323-8638 
 




_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------ 

Message: 5 
Date: Tue, 23 Nov 2010 08:34:29 -0500 
From: Histology Director  
Subject: [Histonet] GU pathology laboratory looking for histotechs/ 
????????cytotechs in Columbus, Ohio 
To: histonet  
Message-ID: 
???????? 
Content-Type: text/plain; charset=ISO-8859-1 

Looking for experienced histology technicians and cytotechnologists in 
Columbus, Ohio for new GU pathology laboratory. Excellent compensation with 
great benefits. Experience with IHC, FISH, and GU pathology a plus. Send 
resume or CV to careers@aksm.com. 


------------------------------ 

Message: 6 
Date: Tue, 23 Nov 2010 08:41:01 -0600 
From: "Hale, Meredith"  
Subject: [Histonet] HT Position 
To:  
Message-ID: 
????????<6F33D8418806044682A391273399860F0610502E@s-irv-ex301.PathologyPartners.intranet> 
???????? 
Content-Type: text/plain;????????charset="us-ascii" 

? 

Great opportunity for a Histotechnician in a brand new laboratory! 
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or 
HTL to run their newly constructed laboratory. Bellmeade Dermatology has 
been in the dermatology business for 18 years with 3 physicians and 2 
Nurse Practitioners' . Candidate must be ASCP certified and CLIA 
certified to perform gross dissection, prior supervisory experience 
preferred. The candidate will be responsible for the following: Creation 
and maintenance of policies and procedures to CLIA standards, leading 
lab through CLIA inspection, maintenance and quality control for 
equipment, and routine histology duties. This is a part time ?position 
that offers a competitive salary and ?the flexible hours allows you to 
put your own personal stamp on the laboratory . ?Interested applicants 
should contact Meredith Hale phone 214-596-2219 or through email 
mhale@carisls.com 

? 

? 

? 

Meredith Hale HT (ASCP) CM 

Operations Liaison Director and Education Coordinator 

? 

Caris Life Sciences 

6655 North MacArthur Blvd, Irving Texas 75039 

direct: 214-596-2219 

cell: 469-648-8253 

fax: 972-929-9966 

mhale@carisls.com  ? 

? 



------------------------------ 

Message: 7 
Date: Tue, 23 Nov 2010 09:50:00 -0500 
From: "Feher, Stephen"  
Subject: RE: [Histonet] ThinPrep vs. SurePath 
To: "Jennifer MacDonald" , 
???????? 
Message-ID: 
????????<0908FC0A43B87A4FB60EDCCA06AABC243868BA@exchange.cmc-nh.org> 
Content-Type: text/plain;????????charset="us-ascii" 

Hi Jennifer, 

I have used both systems, as a cytotech, processing tech, and as a 
supervisor. ?As a cytotech, there is less area of the slide to have to 
review so from that viewpoint it seems more efficient. ?The drawback for 
me was the size of the cells. ?For Gyn specimens, very small high grade 
cells can be difficult to detect without some sort of computer assisted 
system. The Focal Point system was supposed to provide this assistance 
but I found it somewhat unreliable. ?This was further complicated by the 
Focal Point being about 50% accurate in detecting endocervical cells. 
Labs that allowed specimens that automatically signed out cases the 
Focal Point deemed at the lowest risk for abnormal cells frequently had 
clinicians repeating paps due to there being no endocervical material 
detected. ?I found that non-gyn specimens processed using SurePath were 
very difficult for inexperienced techs and many pathologists simply did 
not trust that they were getting the appropriate sampling of cells. 
Very experienced cytotechs and some pathologists preferred SurePath for 
non-gyns but they are in the minority and I found them difficult and 
time consuming to review. ?As a processing tech, SurePath was very time 
consuming and if a lab has any kind of volume at all, had to have at 
least 2 technicians dedicated to nothing but processing them. 

ThinPrep has a larger are to look at and those techs who are used to 
SurePath do not like to review them because the cells are quite a bit 
larger, and if doing manual screening, takes a bit longer to review. 
Use of the ThinPrep Imager has cut screening time down considerably. 
Time and statistical analysis has shown that the Imager is a very 
reliable instrument for indicating those areas of the slide where 
abnormal cells may be found. ?Criticism has been that the Imager 
sometimes misses cells with HPV and viral ?effect. ?Even so, the primary 
focus of many labs is to properly detect and report high grade lesions 
and for this the Imager is reliable. ?For me, non-gyn specimens were 
easier to review and displayed a good distribution of cells. ?A good 
many pathologists agree and are confident that the cell sampling 
adequate. ?As a processing tech, ThinPrep processing takes less time and 
less personnel than SurePath. ?If the T-5000 processor is ever released 
by the FDA for use in the US for gyn specimens, this time will be 
further reduced. 

In the end, the smaller cell distribution size offered by SurePath, 
while seemingly more efficient, requires more time to review for many 
techs due to the small size of the cells. ?ThinPrep currently offers a 
better solution to a majority of labs in terms of accuracy, time to 
prepare, and Imaging. ? 

Hope this was helpful. 


Steve 

-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald 
Sent: Wednesday, November 10, 2010 6:20 PM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] ThinPrep vs. SurePath 

Does anyone have an opinion as to why there seems to be many more 
ThinPreps out there than Sure Path? ?The SurePath seems more efficient, 
but I might be missing something. 
Thank you, 
Jennifer MacDonald 
_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



------------------------------ 

Message: 8 
Date: Tue, 23 Nov 2010 10:28:23 -0600 
From: "Nails, Felton"  
Subject: [Histonet] C3D IF 
To: "'Laurie Colbert'" , 
????????"godsgalnow@aol.com" , 
????????"Histonet@lists.utsouthwestern.edu" 
???????? 
Message-ID: 
???????? 
???????? 
Content-Type: text/plain; charset=us-ascii 

Is anyone perform immunofluorescence for C3D? 

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------------------------------ 

Message: 9 
Date: Tue, 23 Nov 2010 12:09:09 -0500 
From: "Bell, Lynne"  
Subject: [Histonet] HistoGel for cell blocks 
To: "Histonet (histonet@lists.utsouthwestern.edu)" 
???????? 
Message-ID: 
???????? 
Content-Type: text/plain; charset="us-ascii" 

One of my pathologists is interested in the pros and cons of HistoGel for cell blocks. ?I have read the archives and gathered some information for him. ?He specifically would like to talk to someone that has been using it for some time. 

Along the same line, what method do you find to be the best for cell blocks? 

Any help would be appreciated. ?Thanks! 

Lynne Bell, HT (ASCP) 
Histology Team Leader 
Central Vermont Medical Center 
P. O. Box 547 
Barre, VT ?05641 
802-371-4923 




------------------------------ 

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Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

End of Histonet Digest, Vol 84, Issue 28 
**************************************** 
From jana.verbeek <@t> spectrum-health.org  Fri Nov 26 06:59:37 2010
From: jana.verbeek <@t> spectrum-health.org (jana.verbeek@spectrum-health.org)
Date: Fri Nov 26 06:59:44 2010
Subject: [Histonet] hydrometer
Message-ID: <9DAD0B839EC07948A1E942871164053C08E2A63D0D@DVMSXVS06.Spectrum-Health.org>

CBG Biotech sells hydrometers.
There item number is MIS1003.....$45.
1-800-941-9484

Jana Ver Beek, HT(ASCP)
Spectrum Health-Blodgett Campus
Grand Rapids, MI 49506
From kim.tournear <@t> yahoo.com  Fri Nov 26 09:02:40 2010
From: kim.tournear <@t> yahoo.com (Kim Tournear)
Date: Fri Nov 26 09:02:44 2010
Subject: [Histonet] hydrometers
Message-ID: <453863.13492.qm@smtp115-mob.biz.mail.ne1.yahoo.com>

E.C. Kraus has hydrometers for $15.00

From POWELL_SA <@t> mercer.edu  Fri Nov 26 11:52:24 2010
From: POWELL_SA <@t> mercer.edu (Shirley A. Powell)
Date: Fri Nov 26 11:53:54 2010
Subject: [Histonet] cheap metal detector
In-Reply-To: <20101124.210422.22953.0@webmail12.dca.untd.com>
References: <20101124.210422.22953.0@webmail12.dca.untd.com>
Message-ID: <9BF995BC0E47744E9673A41486E24EE226919557F1@MERCERMAIL.MercerU.local>

Here is a website for cheap metal detectors for those who need to find staples in specimens for their pathologists.
Shirley

http://www.woodcraft.com/Product/2003820/9451/Little-Wizard-Metal-Detector.aspx
From me <@t> ccalexander.com  Fri Nov 26 13:41:54 2010
From: me <@t> ccalexander.com (Alex Cummins)
Date: Fri Nov 26 13:41:59 2010
Subject: [Histonet] embyro embeding
Message-ID: <015201cb8da1$fbb44ea0$f31cebe0$@com>

I am cutting whole embryos and neonates. I would prefer to avoid paraffin (
I seem to have issues with varying consistency of tissue between different
organs. Ie some are very friable while others cut perfectly all in the same
block) and cut frozen on a sliding microtome. However I need to keep the
pieces from separating and keep them in the proper orientation. I was going
to use gelatin but the pieces fall out even when I vacuum embed  and fix the
gelatin. I have just joined the list so I don't know all the passed posts. I
did see some about HistoGel. Would this allow me to embed with histogel and
cut frozen on a sliding microtome and retain a "complete section " that I
could then mount without losing pieces? 

Also If I use Histogel first would this improve the issues I have with
paraffin. 

Lastly has anyone cut paraffin sections on a sliding microtome. How do you
set it up? Blade angle , Perpendicular to block or angled?

 

Thanks

Alex

From koellingr <@t> comcast.net  Fri Nov 26 15:08:06 2010
From: koellingr <@t> comcast.net (koellingr@comcast.net)
Date: Fri Nov 26 15:08:12 2010
Subject: [Histonet] CD20 for IHC on  mouse FFPE tissue
In-Reply-To: <201011250631.27710.amosbrooks@gmail.com>
Message-ID: <827537357.1521764.1290805686840.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net>



Hello, 

Maybe I'm remembering wrong since I deleted the original message of Bretts looking for murine CD20 on mouse FFPE (hopefully rabbit origin?). If so anti-murine CD20 that can be made to work on mouse? FFPE?tissues certainly exist. Have used them from BD (flow reagent), Santa Cruz and others.? In Biocompare website, can see them plus new rabbit monoclonal anti-murine CD20 for paraffin, reactive to murine tissues.??There are?a few CD20+ murine B-cells that have no B220, and vice-versa so I would'nt look for CD45R if I wanted to find?CD20+ cells. Would look for CD20+ cells. Maybe I'm remembering original post incorrectly. 

Ray 



Ray Koelling 

PhenoPath Labs 

Seattle, WA 

? 
----- Original Message ----- 
From: "Amos Brooks"  
To: "brett connolly" , histonet@lists.utsouthwestern.edu 
Sent: Thursday, November 25, 2010 3:31:27 AM 
Subject: [Histonet] CD20 for IHC on ?mouse FFPE tissue 

Hi Brett, 
????????CD20 is not going to work for mouse tissue. You need CD45R (clone B220). This 
will selectively label B cells just like CD20 in humans. I get this from BD 
Biosciences (Now Thermo ... again) Catalog # 347460. It is raised in Rats and 
works great as long as you have a good rat secondary. The one from Jackson is 
good. 

Happy Thanksgiving, 
Amos 

_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
From amosbrooks <@t> gmail.com  Fri Nov 26 15:32:49 2010
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Fri Nov 26 15:33:26 2010
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
In-Reply-To: 
References: <4ced52d7.ad05ec0a.583f.640fSMTPIN_ADDED@mx.google.com>
	<201011250614.56234.amosbrooks@gmail.com>
	
Message-ID: <201011261632.49836.amosbrooks@gmail.com>

Wow Bryan,
	That's pretty slick. Obviously weighing it would do the trick I hadn't 
thought of that. I'll have to remember that the next time I mindlessly try to 
shake the excess liquid off my hydrometer and have it break in my hands. Gosh 
I hated that! That is definitely a great and probably more precise way of 
doing it.

Amos


On Thursday 25 November 2010 03:11:07 pm Bryan Llewellyn wrote:
> Specific gravity is mass/volume.  In this context that is grams/millilitre.
> It can easily be measured without a hydrometer.
>
> 1.  Obtain a 10 ml beaker and weigh it to 2 decimal places.
> 2.  Measure 10 mL of the alcohol with a volumetric pipette and place in the
> beaker.
> 3.  Reweigh the beaker with the alcohol in it, again to 2 decimal places.
> 4.  Subtract the weight of the beaker from the weight of the beaker and
> alcohol, giving the weight of the alcohol
> 5.  Divide the weight of the alcohol by 10 to get the SG to 3 decimal
> places.
>
> Bryan Llewellyn
>
>
> ----- Original Message -----
> From: "Amos Brooks" 
> To: ; 
> Sent: Thursday, November 25, 2010 3:14 AM
> Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
>
>
> Hi Jim,
>      Hydrometers can get really expensive. I searched around for one with a
> good price and stumbled on this one from Cole Parmer (now Thermo like
> everyone else in the world):
> Thermo Scientific ERTCO? Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200
> Proof,
> Plain Form ... CAT# EW-08285-00
> I picked it up for $29.50, but that was with my University discount. I'm
> not sure what regular price is or what discounts you might be able to get.
> It sure
> beat the heck out of some of these $200 ones out there. This one has both
> ETOH
> percentages as well as ETOH proofs. It works well for us.
>
> Happy Thanksgiving,
> Amos
>
>
> Message: 4
> Date: Tue, 23 Nov 2010 13:02:24 -0600
> From: "Vickroy, Jim" 
> Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
> To: "histonet@lists.utsouthwestern.edu"
> 
> Message-ID:
> <24A4826E8EF0964D86BC5317306F58A55510FE3506@mmc-
> mail.ad.mhsil.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone know where we can purchase a hydrometer or other instrument 
for
> confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in
> chemicals on a processor and I am going to be asked about instruments to
> confirm percentages before processing.
>
> Meeting with risk management tomorrow.
>
>
> James Vickroy BS, HT(ASCP)
>
> Surgical and Autopsy Pathology Technical Supervisor
> Memorial Medical Center
> 217-788-4046
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From amosbrooks <@t> gmail.com  Fri Nov 26 15:57:32 2010
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Fri Nov 26 15:58:15 2010
Subject: [Histonet] CD20 for IHC on  mouse FFPE tissue
In-Reply-To: <827537357.1521764.1290805686840.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net>
References: <827537357.1521764.1290805686840.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net>
Message-ID: <201011261657.32798.amosbrooks@gmail.com>

Thanks Brett,
	Looks like I got a couple lines crossed there. I had been told that there 
wasn't any CD20 in mice. I have been using B220 for BCell detection. After you 
pointed out the error I looked it up at Biocompare and clearly there are some 
CD20 markers for mice. http://tinyurl.com/38wyh42
	I was also apparently wrong about BD being a Thermo company.  Thanks 
Peter. They do distribute it, but don't own it. My mistake ... again :-) With 
those goofs I'll shut up now and blame the turkey

Enjoy your weekend guys,
Amos


On Friday 26 November 2010 04:08:06 pm koellingr@comcast.net wrote:
> Hello,
>
> Maybe I'm remembering wrong since I deleted the original message of Bretts
> looking for murine CD20 on mouse FFPE (hopefully rabbit origin?). If so
> anti-murine CD20 that can be made to work on mouse? FFPE?tissues certainly
> exist. Have used them from BD (flow reagent), Santa Cruz and others.? In
> Biocompare website, can see them plus new rabbit monoclonal anti-murine
> CD20 for paraffin, reactive to murine tissues.??There are?a few CD20+
> murine B-cells that have no B220, and vice-versa so I would'nt look for
> CD45R if I wanted to find?CD20+ cells. Would look for CD20+ cells. Maybe
> I'm remembering original post incorrectly.
>
> Ray
>
>
>
> Ray Koelling
>
> PhenoPath Labs
>
> Seattle, WA
>
> ?
> ----- Original Message -----
> From: "Amos Brooks" 
> To: "brett connolly" ,
> histonet@lists.utsouthwestern.edu Sent: Thursday, November 25, 2010 3:31:27
> AM
> Subject: [Histonet] CD20 for IHC on ?mouse FFPE tissue
>
> Hi Brett,
> ????????CD20 is not going to work for mouse tissue. You need CD45R (clone
> B220). This will selectively label B cells just like CD20 in humans. I get
> this from BD Biosciences (Now Thermo ... again) Catalog # 347460. It is
> raised in Rats and works great as long as you have a good rat secondary.
> The one from Jackson is good.
>
> Happy Thanksgiving,
> Amos
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From histologydirector <@t> gmail.com  Fri Nov 26 17:53:19 2010
From: histologydirector <@t> gmail.com (Histology Director)
Date: Fri Nov 26 17:53:23 2010
Subject: [Histonet] GU pathology laboratory looking for histotechs/
 cytotechs in Columbus, Ohio
Message-ID: 

Looking for experienced histology technicians and cytotechnologists in
Columbus, Ohio for new GU pathology laboratory. Excellent compensation with
great benefits. Experience with IHC, FISH, and GU pathology a plus. Send
resume or CV to careers@aksm.com.
From rjbuesa <@t> yahoo.com  Sun Nov 28 08:20:05 2010
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Sun Nov 28 08:20:14 2010
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
In-Reply-To: <201011261632.49836.amosbrooks@gmail.com>
Message-ID: <775501.80634.qm@web65707.mail.ac4.yahoo.com>

Amos:
Bryan's method has 2 problems:
1- It requires a volumetric flask (one that assures a constant volume and it does that because the glass cap has a small hole that allows overflow of excess liquid), and
2- it will tell you the density of the liquid you placed inside the flask but NOT its strength, in this case the amount of water the alcohol contains. That would require a table to compare the density you calculated against that of known alcoholic dilutions.??
For the level of accuracy needed in tissue processing, any hydrometer will suffice, and you don't have to shake it, just wipe it dry with a towel.
Ren? J.

--- On Fri, 11/26/10, Amos Brooks  wrote:


From: Amos Brooks 
Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
To: "Bryan Llewellyn" 
Cc: "Histonet" 
Date: Friday, November 26, 2010, 4:32 PM


Wow Bryan,
??? That's pretty slick. Obviously weighing it would do the trick I hadn't 
thought of that. I'll have to remember that the next time I mindlessly try to 
shake the excess liquid off my hydrometer and have it break in my hands. Gosh 
I hated that! That is definitely a great and probably more precise way of 
doing it.

Amos


On Thursday 25 November 2010 03:11:07 pm Bryan Llewellyn wrote:
> Specific gravity is mass/volume.? In this context that is grams/millilitre.
> It can easily be measured without a hydrometer.
>
> 1.? Obtain a 10 ml beaker and weigh it to 2 decimal places.
> 2.? Measure 10 mL of the alcohol with a volumetric pipette and place in the
> beaker.
> 3.? Reweigh the beaker with the alcohol in it, again to 2 decimal places.
> 4.? Subtract the weight of the beaker from the weight of the beaker and
> alcohol, giving the weight of the alcohol
> 5.? Divide the weight of the alcohol by 10 to get the SG to 3 decimal
> places.
>
> Bryan Llewellyn
>
>
> ----- Original Message -----
> From: "Amos Brooks" 
> To: ; 
> Sent: Thursday, November 25, 2010 3:14 AM
> Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
>
>
> Hi Jim,
>? ? ? Hydrometers can get really expensive. I searched around for one with a
> good price and stumbled on this one from Cole Parmer (now Thermo like
> everyone else in the world):
> Thermo Scientific ERTCO? Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200
> Proof,
> Plain Form ... CAT# EW-08285-00
> I picked it up for $29.50, but that was with my University discount. I'm
> not sure what regular price is or what discounts you might be able to get.
> It sure
> beat the heck out of some of these $200 ones out there. This one has both
> ETOH
> percentages as well as ETOH proofs. It works well for us.
>
> Happy Thanksgiving,
> Amos
>
>
> Message: 4
> Date: Tue, 23 Nov 2010 13:02:24 -0600
> From: "Vickroy, Jim" 
> Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
> To: "histonet@lists.utsouthwestern.edu"
> 
> Message-ID:
> <24A4826E8EF0964D86BC5317306F58A55510FE3506@mmc-
> mail.ad.mhsil.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone know where we can purchase a hydrometer or other instrument 
for
> confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in
> chemicals on a processor and I am going to be asked about instruments to
> confirm percentages before processing.
>
> Meeting with risk management tomorrow.
>
>
> James Vickroy BS, HT(ASCP)
>
> Surgical and Autopsy Pathology Technical Supervisor
> Memorial Medical Center
> 217-788-4046
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From llewllew <@t> shaw.ca  Sun Nov 28 09:46:52 2010
From: llewllew <@t> shaw.ca (Bryan Llewellyn)
Date: Sun Nov 28 09:47:22 2010
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
In-Reply-To: <775501.80634.qm@web65707.mail.ac4.yahoo.com>
References: <775501.80634.qm@web65707.mail.ac4.yahoo.com>
Message-ID: <2928EE921AF440E5B12C837507145AFC@BryanPC>

Your first objection is not the case.  The procedure I outlined used a 10 mL volumetric pipette, not a flask, and a plain beaker.  These are extremely common in medical laboratories and accurate enough for the purpose.

Your second objection is quite true, but really, how difficult is it to make one time alcohol dilutions and weigh them, then plot the concentrations against either the weights or the specific gravity on a graph.  For occasional use it works extremely well.  On the other hand, if you are doing it many times a day, then a densitometer with direct readings of acohol concentrations should be used.

Bryan Llewellyn

  ----- Original Message ----- 
  From: Rene J Buesa 
  To: Bryan Llewellyn ; Amos Brooks 
  Cc: Histonet 
  Sent: Sunday, November 28, 2010 6:20 AM
  Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS


        Amos:
        Bryan's method has 2 problems:
        1- It requires a volumetric flask (one that assures a constant volume and it does that because the glass cap has a small hole that allows overflow of excess liquid), and
        2- it will tell you the density of the liquid you placed inside the flask but NOT its strength, in this case the amount of water the alcohol contains. That would require a table to compare the density you calculated against that of known alcoholic dilutions.  
        For the level of accuracy needed in tissue processing, any hydrometer will suffice, and you don't have to shake it, just wipe it dry with a towel.
        Ren? J.

        --- On Fri, 11/26/10, Amos Brooks  wrote:


          From: Amos Brooks 
          Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
          To: "Bryan Llewellyn" 
          Cc: "Histonet" 
          Date: Friday, November 26, 2010, 4:32 PM


          Wow Bryan,
              That's pretty slick. Obviously weighing it would do the trick I hadn't 
          thought of that. I'll have to remember that the next time I mindlessly try to 
          shake the excess liquid off my hydrometer and have it break in my hands. Gosh 
          I hated that! That is definitely a great and probably more precise way of 
          doing it.

          Amos


          On Thursday 25 November 2010 03:11:07 pm Bryan Llewellyn wrote:
          > Specific gravity is mass/volume.  In this context that is grams/millilitre.
          > It can easily be measured without a hydrometer.
          >
          > 1.  Obtain a 10 ml beaker and weigh it to 2 decimal places.
          > 2.  Measure 10 mL of the alcohol with a volumetric pipette and place in the
          > beaker.
          > 3.  Reweigh the beaker with the alcohol in it, again to 2 decimal places.
          > 4.  Subtract the weight of the beaker from the weight of the beaker and
          > alcohol, giving the weight of the alcohol
          > 5.  Divide the weight of the alcohol by 10 to get the SG to 3 decimal
          > places.
          >
          > Bryan Llewellyn
          >
          >
          > ----- Original Message -----
          > From: "Amos Brooks" 
          > To: ; 
          > Sent: Thursday, November 25, 2010 3:14 AM
          > Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
          >
          >
          > Hi Jim,
          >      Hydrometers can get really expensive. I searched around for one with a
          > good price and stumbled on this one from Cole Parmer (now Thermo like
          > everyone else in the world):
          > Thermo Scientific ERTCO? Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200
          > Proof,
          > Plain Form ... CAT# EW-08285-00
          > I picked it up for $29.50, but that was with my University discount. I'm
          > not sure what regular price is or what discounts you might be able to get.
          > It sure
          > beat the heck out of some of these $200 ones out there. This one has both
          > ETOH
          > percentages as well as ETOH proofs. It works well for us.
          >
          > Happy Thanksgiving,
          > Amos
          >
          >
          > Message: 4
          > Date: Tue, 23 Nov 2010 13:02:24 -0600
          > From: "Vickroy, Jim" 
          > Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
          > To: "histonet@lists.utsouthwestern.edu"
          > 
          > Message-ID:
          > <24A4826E8EF0964D86BC5317306F58A55510FE3506@mmc-
          > mail.ad.mhsil.com>
          > Content-Type: text/plain; charset="us-ascii"
          >
          > Does anyone know where we can purchase a hydrometer or other instrument 
          for
          > confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in
          > chemicals on a processor and I am going to be asked about instruments to
          > confirm percentages before processing.
          >
          > Meeting with risk management tomorrow.
          >
          >
          > James Vickroy BS, HT(ASCP)
          >
          > Surgical and Autopsy Pathology Technical Supervisor
          > Memorial Medical Center
          > 217-788-4046
          >
          > _______________________________________________
          > Histonet mailing list
          > Histonet@lists.utsouthwestern.edu
          > http://lists.utsouthwestern.edu/mailman/listinfo/histonet


          _______________________________________________
          Histonet mailing list
          Histonet@lists.utsouthwestern.edu
          http://lists.utsouthwestern.edu/mailman/listinfo/histonet
       

From llewllew <@t> shaw.ca  Sun Nov 28 13:38:45 2010
From: llewllew <@t> shaw.ca (Bryan Llewellyn)
Date: Sun Nov 28 13:39:15 2010
Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
In-Reply-To: 
References: <775501.80634.qm@web65707.mail.ac4.yahoo.com>
	<2928EE921AF440E5B12C837507145AFC@BryanPC>
	
Message-ID: <0F6586BBA7A44E3B9BC7B985346A9258@BryanPC>

This is what I meant: http://en.wikipedia.org/wiki/Volumetric_pipettes

Bryan Llewellyn

  ----- Original Message ----- 
  From: Lynn Lee 
  To: llewllew@shaw.ca 
  Sent: Sunday, November 28, 2010 7:53 AM
  Subject: RE: [Histonet] CHECKING ALCOHOL CONCENTRATIONS



  Do you mean a calibrated pipette?
   
  L. Lee, Tucson, AZ



   
  > From: llewllew@shaw.ca
  > To: histonet@lists.utsouthwestern.edu; rjbuesa@yahoo.com
  > Date: Sun, 28 Nov 2010 07:46:52 -0800
  > Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
  > CC: 
  > 
  > Your first objection is not the case. The procedure I outlined used a 10 mL volumetric pipette, not a flask, and a plain beaker. These are extremely common in medical laboratories and accurate enough for the purpose.
  > 
  > Your second objection is quite true, but really, how difficult is it to make one time alcohol dilutions and weigh them, then plot the concentrations against either the weights or the specific gravity on a graph. For occasional use it works extremely well. On the other hand, if you are doing it many times a day, then a densitometer with direct readings of acohol concentrations should be used.
  > 
  > Bryan Llewellyn
  > 
  > ----- Original Message ----- 
  > From: Rene J Buesa 
  > To: Bryan Llewellyn ; Amos Brooks 
  > Cc: Histonet 
  > Sent: Sunday, November 28, 2010 6:20 AM
  > Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
  > 
  > 
  > Amos:
  > Bryan's method has 2 problems:
  > 1- It requires a volumetric flask (one that assures a constant volume and it does that because the glass cap has a small hole that allows overflow of excess liquid), and
  > 2- it will tell you the density of the liquid you placed inside the flask but NOT its strength, in this case the amount of water the alcohol contains. That would require a table to compare the density you calculated against that of known alcoholic dilutions. 
  > For the level of accuracy needed in tissue processing, any hydrometer will suffice, and you don't have to shake it, just wipe it dry with a towel.
  > Ren? J.
  > 
  > --- On Fri, 11/26/10, Amos Brooks  wrote:
  > 
  > 
  > From: Amos Brooks 
  > Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
  > To: "Bryan Llewellyn" 
  > Cc: "Histonet" 
  > Date: Friday, November 26, 2010, 4:32 PM
  > 
  > 
  > Wow Bryan,
  > That's pretty slick. Obviously weighing it would do the trick I hadn't 
  > thought of that. I'll have to remember that the next time I mindlessly try to 
  > shake the excess liquid off my hydrometer and have it break in my hands. Gosh 
  > I hated that! That is definitely a great and probably more precise way of 
  > doing it.
  > 
  > Amos
  > 
  > 
  > On Thursday 25 November 2010 03:11:07 pm Bryan Llewellyn wrote:
  > > Specific gravity is mass/volume. In this context that is grams/millilitre.
  > > It can easily be measured without a hydrometer.
  > >
  > > 1. Obtain a 10 ml beaker and weigh it to 2 decimal places.
  > > 2. Measure 10 mL of the alcohol with a volumetric pipette and place in the
  > > beaker.
  > > 3. Reweigh the beaker with the alcohol in it, again to 2 decimal places.
  > > 4. Subtract the weight of the beaker from the weight of the beaker and
  > > alcohol, giving the weight of the alcohol
  > > 5. Divide the weight of the alcohol by 10 to get the SG to 3 decimal
  > > places.
  > >
  > > Bryan Llewellyn
  > >
  > >
  > > ----- Original Message -----
  > > From: "Amos Brooks" 
  > > To: ; 
  > > Sent: Thursday, November 25, 2010 3:14 AM
  > > Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
  > >
  > >
  > > Hi Jim,
  > > Hydrometers can get really expensive. I searched around for one with a
  > > good price and stumbled on this one from Cole Parmer (now Thermo like
  > > everyone else in the world):
  > > Thermo Scientific ERTCO? Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200
  > > Proof,
  > > Plain Form ... CAT# EW-08285-00
  > > I picked it up for $29.50, but that was with my University discount. I'm
  > > not sure what regular price is or what discounts you might be able to get.
  > > It sure
  > > beat the heck out of some of these $200 ones out there. This one has both
  > > ETOH
  > > percentages as well as ETOH proofs. It works well for us.
  > >
  > > Happy Thanksgiving,
  > > Amos
  > >
  > >
  > > Message: 4
  > > Date: Tue, 23 Nov 2010 13:02:24 -0600
  > > From: "Vickroy, Jim" 
  > > Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
  > > To: "histonet@lists.utsouthwestern.edu"
  > > 
  > > Message-ID:
  > > <24A4826E8EF0964D86BC5317306F58A55510FE3506@mmc-
  > > mail.ad.mhsil.com>
  > > Content-Type: text/plain; charset="us-ascii"
  > >
  > > Does anyone know where we can purchase a hydrometer or other instrument 
  > for
  > > confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in
  > > chemicals on a processor and I am going to be asked about instruments to
  > > confirm percentages before processing.
  > >
  > > Meeting with risk management tomorrow.
  > >
  > >
  > > James Vickroy BS, HT(ASCP)
  > >
  > > Surgical and Autopsy Pathology Technical Supervisor
  > > Memorial Medical Center
  > > 217-788-4046
  > >
  > > _______________________________________________
  > > Histonet mailing list
  > > Histonet@lists.utsouthwestern.edu
  > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  > 
  > 
  > _______________________________________________
  > Histonet mailing list
  > Histonet@lists.utsouthwestern.edu
  > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  > 
  > 
  > _______________________________________________
  > Histonet mailing list
  > Histonet@lists.utsouthwestern.edu
  > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From histopathy <@t> hotmail.com  Sun Nov 28 14:38:02 2010
From: histopathy <@t> hotmail.com (Pamela Gholston)
Date: Sun Nov 28 14:38:07 2010
Subject: [Histonet] Nails softened up during grossing process or at the
	microtome bench?
Message-ID: 


Hello to everyone.  What is the general concensus on softening up of nails before cutting?  Does anyone have a process that the gross tech follows or is it done at the microtome bench?  

Histopathy

 		 	   		  
From rjbuesa <@t> yahoo.com  Sun Nov 28 14:45:00 2010
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Sun Nov 28 14:45:04 2010
Subject: [Histonet] Nails softened up during grossing process or at the
	microtome bench?
In-Reply-To: 
Message-ID: <800896.13183.qm@web65716.mail.ac4.yahoo.com>

Should be softened BEFORE tissue processing using 10% aq. sol. of sodium hydroxide during 30 min ? wash ? process as usual.
Ren? J.

--- On Sun, 11/28/10, Pamela Gholston  wrote:



From: Pamela Gholston 
Subject: [Histonet] Nails softened up during grossing process or at the microtome bench?
To: histonet@lists.utsouthwestern.edu
Date: Sunday, November 28, 2010, 3:38 PM



Hello to everyone.? What is the general concensus on softening up of nails before cutting?? Does anyone have a process that the gross tech follows or is it done at the microtome bench?? 

Histopathy

??? ???????? ?????? ??? ? _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From sgoebel <@t> mirnarx.com  Mon Nov 29 08:31:11 2010
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Mon Nov 29 08:31:15 2010
Subject: [Histonet] Re: Histology Consultant
In-Reply-To: 
References: 
Message-ID: 

Thanks Nathan!!  I just don't want to be cheated on what I will be
getting paid.  My old job is wanting me to come in and do the small
amount of histology work they have.  This would all be on evenings and
weekends.  It is not PRN, more like call me once a month and I will come
do the work.  I'm getting anywhere from $17/hr (that's insane!!  How do
you even sleep at night paying a registered, college educated person
that? Working at Walmart would pay you more!!!) to $50/hr (which was
more what I was thinking).  Just trying to get a good feel, and not
cheat myself or the other company.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nathan
Jentsch
Sent: Thursday, November 25, 2010 6:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histology Consultant

If you don't know what the going rate is, that is exactly why you ask.
That
is what this forum is for.  A little professionalism is appreciated.

Nathan Jentsch
BS HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From brett_connolly <@t> merck.com  Mon Nov 29 08:32:14 2010
From: brett_connolly <@t> merck.com (Connolly, Brett M)
Date: Mon Nov 29 08:32:21 2010
Subject: [Histonet] CD20 for IHC on  mouse FFPE tissue
In-Reply-To: <201011261657.32798.amosbrooks@gmail.com>
References: <827537357.1521764.1290805686840.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net>
	<201011261657.32798.amosbrooks@gmail.com>
Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D752C5AB@usctmx1176.merck.com>

Thanks Guys,
I  plan to order one of the Rb monoclonals for CD20.

Best,
Brett

-----Original Message-----
From: Amos Brooks [mailto:amosbrooks@gmail.com] 
Sent: Friday, November 26, 2010 4:58 PM
To: koellingr@comcast.net
Cc: Connolly, Brett M; histonet@lists.utsouthwestern.edu; Peter.Tree@abdserotec.com
Subject: Re: [Histonet] CD20 for IHC on mouse FFPE tissue

Thanks Brett,
	Looks like I got a couple lines crossed there. I had been told that there 
wasn't any CD20 in mice. I have been using B220 for BCell detection. After you 
pointed out the error I looked it up at Biocompare and clearly there are some 
CD20 markers for mice. http://tinyurl.com/38wyh42
	I was also apparently wrong about BD being a Thermo company.  Thanks 
Peter. They do distribute it, but don't own it. My mistake ... again :-) With 
those goofs I'll shut up now and blame the turkey

Enjoy your weekend guys,
Amos


On Friday 26 November 2010 04:08:06 pm koellingr@comcast.net wrote:
> Hello,
>
> Maybe I'm remembering wrong since I deleted the original message of Bretts
> looking for murine CD20 on mouse FFPE (hopefully rabbit origin?). If so
> anti-murine CD20 that can be made to work on mouse? FFPE?tissues certainly
> exist. Have used them from BD (flow reagent), Santa Cruz and others.? In
> Biocompare website, can see them plus new rabbit monoclonal anti-murine
> CD20 for paraffin, reactive to murine tissues.??There are?a few CD20+
> murine B-cells that have no B220, and vice-versa so I would'nt look for
> CD45R if I wanted to find?CD20+ cells. Would look for CD20+ cells. Maybe
> I'm remembering original post incorrectly.
>
> Ray
>
>
>
> Ray Koelling
>
> PhenoPath Labs
>
> Seattle, WA
>
> ?
> ----- Original Message -----
> From: "Amos Brooks" 
> To: "brett connolly" ,
> histonet@lists.utsouthwestern.edu Sent: Thursday, November 25, 2010 3:31:27
> AM
> Subject: [Histonet] CD20 for IHC on ?mouse FFPE tissue
>
> Hi Brett,
> ????????CD20 is not going to work for mouse tissue. You need CD45R (clone
> B220). This will selectively label B cells just like CD20 in humans. I get
> this from BD Biosciences (Now Thermo ... again) Catalog # 347460. It is
> raised in Rats and works great as long as you have a good rat secondary.
> The one from Jackson is good.
>
> Happy Thanksgiving,
> Amos
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From wdesalvo.cac <@t> hotmail.com  Mon Nov 29 09:31:46 2010
From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO)
Date: Mon Nov 29 09:31:51 2010
Subject: [Histonet] Re: Histology Consultant
In-Reply-To: 
References: ,
	
Message-ID: 


Your situation sounds more like "contract" work instead of "consultation" work. Your have a right to expect and I suggest you must demand a fair hourly rate to compensate for your education, experience, time and effort. You must take into consideration the market hourly rate for the area your work and the degree of difficulty in labs acquiring quality technicians/technologists. In my experience, I believe that $30.00 - $33.00/hr base rate for routine Histology work is not unusual for a contract hire without benefits. I would also suggest that you set a minimum hourly guarantee of 4 hours pay each time you come in to work and negotiate for a shift differential of 10%-15% for working nights.
 
Remember, you are producing work that the lab or pathologist will be able to charge a minimum of $100.00/case, depending on the CPT code level and not counting the number of special stains or IHC attached to each case. You should have no issue in producing no less than 40 cases in a 4 hour period (embedding, cutting and staining). You must ask for fair compensation and always provide superior quality. Your contribution to the process is essential.
 
Additionally, if the lab was to hire a contract person from one of the placement services, they would be charged $60.00-$65.00/hr and have to make a set time commitment (typically 1-3 months). You are a known performer to the lab and they have examples of your quality, they do not have that level of knowledge or comfort if they hire an unknown contractor. 
 
You and all of us in our Histotechnology profession, as registered, trained, competent and educated technologists should expect and demand to be fairly compensated as an essential and professional member of the Anatomic Pathology lab process.

William DeSalvo, B.S., HTL(ASCP)




 
> Date: Mon, 29 Nov 2010 08:31:11 -0600
> From: sgoebel@mirnarx.com
> To: njblademaster@gmail.com; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: Histology Consultant
> CC: 
> 
> Thanks Nathan!! I just don't want to be cheated on what I will be
> getting paid. My old job is wanting me to come in and do the small
> amount of histology work they have. This would all be on evenings and
> weekends. It is not PRN, more like call me once a month and I will come
> do the work. I'm getting anywhere from $17/hr (that's insane!! How do
> you even sleep at night paying a registered, college educated person
> that? Working at Walmart would pay you more!!!) to $50/hr (which was
> more what I was thinking). Just trying to get a good feel, and not
> cheat myself or the other company.
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nathan
> Jentsch
> Sent: Thursday, November 25, 2010 6:51 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Histology Consultant
> 
> If you don't know what the going rate is, that is exactly why you ask.
> That
> is what this forum is for. A little professionalism is appreciated.
> 
> Nathan Jentsch
> BS HT(ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From MSHERWOOD <@t> PARTNERS.ORG  Mon Nov 29 09:41:38 2010
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Mon Nov 29 09:41:47 2010
Subject: [Histonet] Cryostat Question Please
In-Reply-To: 
References: 
	
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5251@PHSXMB30.partners.org>

We have this model as well and like it.  We got the vacuum attachment.  It's not
perfect, but does facilitate cleaning up OCT and tissue scraps.



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Wednesday, November 24, 2010 4:14 PM
To: Paula Lucas; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cryostat Question Please

Very consistant, easy to use. 

2 drawbacks  

1. We got the decontamination add-on trying to automate the CAP
requirement, but now it seems Thermo says it is not an adequate process.
I believe they are doing more intense studies, but for the time being, I
have to decontaminate it by hand. 

2. OCT and tissue scraps build up under collection device and cause the
mechanism to "clunk" unless buildup is removed. 

These two things add a good chunk of time to my day.

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Wednesday, November 24, 2010 1:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Question Please

Hello all
 
Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat?  If so,
what is your opinion/pros and cons?
 
Thanks so much in advance,
Paula
Lab Manager
BP Medical Group
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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The information in this e-mail is intended only for the person to whom it is
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From histologydirector <@t> gmail.com  Mon Nov 29 10:00:05 2010
From: histologydirector <@t> gmail.com (Histology Director)
Date: Mon Nov 29 10:00:09 2010
Subject: [Histonet] Histotechs and cytotechs wanted
Message-ID: 

Looking for experienced histology technicians and cytotechnologists in
Columbus, Ohio for new GU pathology laboratory. Excellent compensation with
great benefits. Experience with IHC, FISH, and GU pathology a plus. Send
resume or CV to careers@aksm.com.
From sgoebel <@t> mirnarx.com  Mon Nov 29 15:04:35 2010
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Mon Nov 29 15:04:41 2010
Subject: [Histonet] Processor change
Message-ID: 

How many blocks do you guys normally process before you change the
solutions in the processor?

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

From sbreeden <@t> nmda.nmsu.edu  Mon Nov 29 15:17:58 2010
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Mon Nov 29 15:18:04 2010
Subject: [Histonet] Processor change
In-Reply-To: 
References: 
Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E474A1@nmdamailsvr.nmda.ad.nmsu.edu>

I change solutions after 500 blocks; paraffin after 1500 blocks.

From jstaruk <@t> masshistology.com  Mon Nov 29 15:32:38 2010
From: jstaruk <@t> masshistology.com (jstaruk)
Date: Mon Nov 29 15:32:37 2010
Subject: [Histonet] Processor change
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E474A1@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <841C97AFFF2E4F48B0D13AE1667CC394@JimPC>

Change or rotate?

_______________________
James E. Staruk HT(ASCP)
 www.masshistology.com
   www.nehorselabs.com
 
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Monday, November 29, 2010 4:18 PM
To: sgoebel@mirnarx.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Processor change

I change solutions after 500 blocks; paraffin after 1500 blocks.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From rjbuesa <@t> yahoo.com  Mon Nov 29 15:44:02 2010
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Mon Nov 29 15:44:06 2010
Subject: [Histonet] Processor change
In-Reply-To: 
Message-ID: <415721.28480.qm@web65714.mail.ac4.yahoo.com>

When the processor capacity is reached, I start to rotate the reagents, discarding the first of each group, moving forward the remaining, and replacing with fresh reagents the last.
Ren? J.

--- On Mon, 11/29/10, sgoebel@mirnarx.com  wrote:


From: sgoebel@mirnarx.com 
Subject: [Histonet] Processor change
To: histonet@lists.utsouthwestern.edu
Date: Monday, November 29, 2010, 4:04 PM


How many blocks do you guys normally process before you change the
solutions in the processor?



Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas? 78744

(512)901-0900 ext. 6912



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From laurie.colbert <@t> huntingtonhospital.com  Mon Nov 29 15:49:57 2010
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Mon Nov 29 15:50:01 2010
Subject: [Histonet] Processor change
In-Reply-To: 
Message-ID: <57BE698966D5C54EAE8612E8941D768309FF6E77@EXCHANGE3.huntingtonhospital.com>

Nancy Klemme, can you comment on this specifically for the Tissue Tek
VIP's?  Does Sakura have guidelines for rotating the solutions?
Laurie Colbert

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
sgoebel@mirnarx.com
Sent: Monday, November 29, 2010 1:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor change

How many blocks do you guys normally process before you change the
solutions in the processor?

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From JWeems <@t> sjha.org  Mon Nov 29 16:05:54 2010
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Mon Nov 29 16:05:59 2010
Subject: [Histonet] RE: Processor change
In-Reply-To: 
References: 
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044A0FB9A2@CHEXCMS10.one.ads.che.org>

We change based on runs, not blocks - two times a week.. Change each single solution and first of each series and rotate. J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 

 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com
Sent: Monday, November 29, 2010 16:05
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor change

How many blocks do you guys normally process before you change the solutions in the processor?

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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From amacrostie <@t> hotmail.com  Mon Nov 29 16:43:51 2010
From: amacrostie <@t> hotmail.com (Allison MacRostie)
Date: Mon Nov 29 16:43:54 2010
Subject: [Histonet] Looking for info on SF Bay area jobs
Message-ID: 


Hello all fellow histonet users,
 
I am thinking of relocating to the San Francisco Bay area and was wondering if any users live and work in the area? I am curious about  job opportunities and pay scale and that sort of thing. Any information or advice is greatly appreciated!
 
Thank you,
allison 		 	   		  
From lpwenk <@t> sbcglobal.net  Mon Nov 29 21:16:14 2010
From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk)
Date: Mon Nov 29 21:16:15 2010
Subject: [Histonet] Robert Lott
Message-ID: <17ADE9FDCFFF4D5195E2170584679700@HP2010>

Robert - Please email me at work ( pwenk@beaumont.edu ), how/where I can contact you. 

(Thank you to Histonet for allowing me to use this great resource to contact another histotech.)

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
From njblademaster <@t> gmail.com  Mon Nov 29 21:23:53 2010
From: njblademaster <@t> gmail.com (Nathan Jentsch)
Date: Mon Nov 29 21:24:16 2010
Subject: [Histonet] Re: Nails softened up during grossing process or at the
	microtome bench?
Message-ID: 

I work at a derm lab, and we see 3-5 nail specimens a week on average.  We
use a 10% potassium hydroxide and let them soak for 1-3 hours depending on
the size of the specimen.  Then at the microtome after we face in, we soak
the blocks in 10% ammonium hydroxide for 5-10 minutes, which softens them up
nicely for cutting.  We also use the ammonium hydroxide to soak specimens
with a high keratin content that makes them extremely crispy at the
microtome.

Nathan Jentsch
BS HT(ASCP)
From Allison_Scott <@t> hchd.tmc.edu  Mon Nov 29 23:41:15 2010
From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D)
Date: Mon Nov 29 23:41:18 2010
Subject: [Histonet] Procedure for Xylene and Formalin Monitoring
Message-ID: <1872B4A455B7974391609AD8034C79FC019C19BB@LBEXCH01.hchd.local>

Hello to all in Histoland.  I hope that eveyone had a good Thaksgiving.  Does any one have a procedure for when you have your lab gets  monitored for xylene and formalin levels.  Any help would be appreciated.  I am asking for another supervisor. On the anatomic checklist youhave to have evidence of monitoring.  On the micro checklist you have to have a written procedure. Thanks in advance.
 
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
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From JSilverman <@t> NSHS.edu  Tue Nov 30 09:16:43 2010
From: JSilverman <@t> NSHS.edu (Silverman, Jeffrey)
Date: Tue Nov 30 09:16:59 2010
Subject: [Histonet] Histology opportunities in Bay Shore,
	Long Island New York
Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE41442EA@SYKECHXVS01.nslijhs.net>



We currently have two openings in histology at Southside Hospital in Bay Shore, New York. Southside is a 410 bed full service community hospital which is undergoing rapid growth and expansion adding many tertiary health care services  as part of the North Shore-Long Island Jewish Health care system.

The first position is for a pathologists' assistant, preferably who could also function in a supervisory role in the histology lab. We currently process around 8500 surgical cases per year with an interesting case mix. The three pathologists are lovely people and fun to work with. We have an excellent and personable incumbent  staff in histology. AAPA or ASCP certification as a PA is beneficial but not essential for the talented person with the right combination of training and experience, including some knowledge of basic immunohistochemistry.

The second position is for a histology technician or technologist with good basic embedding, microtomy, and staining skills. This person would function as a second histotech/ lab aide staffing the lab from 8 AM to 4PM. We process from  30 (occasionallY)  up to 120 (usual)  blocks a day currently with a light special stain and IHC load usually, though we do have our busier days. We have automated H and E stainer (Leica XL) and automated special stains and IHC (Dako Artisan). We do not do cytology in house, just staining of 1-3  FNA's a day and  the occasional cell block.

If you are interested in either position, I would look forward to speaking with you at 631 375 4505 or email me at jsilverman@nshs.edu. Please note that I will be unavailable for phone contact from the evening of Dec 2 until the evening of Dec 6.

Jeffrey Silverman HT HTL QIHC (ASCP)
Pathologists's Assistant and Supervisor of  Histology

From relia1 <@t> earthlink.net  Tue Nov 30 09:21:46 2010
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Tue Nov 30 09:21:43 2010
Subject: [Histonet] RELIA Special Job Alert for Managers and Supervisors.
	11/30/10
Message-ID: 

Hi Histonetters!!
I hope you had a great Thanksgiving.  I have several clients that are in
need of managers and supervisors so I thought I would put the word out.
At this point since it is the end of the year my clients are willing to
let you call the shots on how you would like to proceed i.e. interview
now or after the holidays.  Of course these are all permanent full time
positions offering excellent salaries, benefits and relocation
assistance.  
 
Here is a list of my current supervisory and management positions:
Manchester, NH - Histology Supervisor
Modesto, CA - Histology Lab Manager 
Orange/Rockland County, NY - Director of Quality Assurance
Colorado Springs, CO - Histology Supervisor
Baton Rouge, LA - Histology Supervisor
 
If you or anyone you know is interested call me toll free at
866-607-3542 or e-mail me at relia1@earthlink.net  
 
Thanks and Have a Great Day!!
 
Thank You! 
 
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia
 
 

From relia1 <@t> earthlink.net  Tue Nov 30 09:28:57 2010
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Tue Nov 30 09:28:54 2010
Subject: [Histonet] RELIA Special Job Alert - Electron Microscopy Specialist
	- Inside Sales/Technical Support needed can you help? A
	unique opportunity for researchers!
Message-ID: 

Hi Histonetters!!
I have a new opportunity that I want to let you know about.  I am
working with a premier vendor of histology products for the research
laboratory located in beautiful Northern CA.  My client offers an
excellent compensation package great benefits and relocation assistance.
In this position you will be responsible for assisting the Marketing &
Sales Director in sales and marketing activities of all kinds, but in
particular those related to the Life Sciences.  And be responsible for
the growth, management and sales of their Light Microscope and Histology
product lines; 

Requirements:The ideal person will be working in a neuroscience research
lab with histology and TEM experience.  Confocal and fluorescence
experience is a good added plus.

 

Remember at this point since it is the end of the year my clients are
willing to let you call the shots on how you would like to proceed i.e.
interview now or after the holidays. 

If you or anyone you know might be interested in hearing more about this
opportunity please contact Pam Barker at relia1@earthlink.net or
866-607-3542


Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From Margaret.Perry <@t> sdstate.edu  Tue Nov 30 10:25:07 2010
From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret)
Date: Tue Nov 30 10:25:14 2010
Subject: [Histonet] special stains QC
Message-ID: 

Our lab would like to know how you handle the quality issue of verifying that the control material you use for special stains is what it's supposed to be. For example: how do you confirm that bile in a piece of liver used as a Halls control is actually bile?
We use controls for bacteria or viruses that are confirmed  by PCR, plating, or virus isolation.  If possible we like to have the case confirmed by two different tests.   If confirmed we test the paraffin blocks from that case by running a stain on them and the pathologist confirming the test positive or negative.

Margaret Perry HT(ASCP)
Dept of Veterinary and  Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638

From ree3 <@t> leicester.ac.uk  Tue Nov 30 10:50:27 2010
From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.)
Date: Tue Nov 30 10:50:51 2010
Subject: [Histonet] acetone/PAS/GMA
Message-ID: <7722595275A4DD4FA225B92CDBF174A1FDA0AEB8F1@EXC-MBX3.cfs.le.ac.uk>


Has anyone ever performed a PAS on acetone fixed GMA processed tissues, we  are  looking for  fungi in bronchial biopsies.

                                 Many thanks

                                     Richard Edwards
                                           University of Leicester

From aevans3 <@t> lghealth.org  Tue Nov 30 10:52:28 2010
From: aevans3 <@t> lghealth.org (Evans, Andria B)
Date: Tue Nov 30 10:53:53 2010
Subject: [Histonet] Open Histotech Position ~ Lancaster, Pennsylvania
Message-ID: <4182FDF23D7C9948BC41C4C082C3A54F0121E810D29A@MAIL-AG-CLUSTER.lha.org>

 Full-Time; Day shift Position



SUMMARY: Embed, cut, mount, stain, coverslip and label tissue and other materials for diagnostic examination by Pathologist.   Special Stains and IHC experience a plus!



EDUCATION: HS diploma or equivalent (GED).  Must be HT or HTL certified.



ESSENTIAL JOB-RELATED EXPERIENCE:  Six (6) to nine (9) months of hospital histology experience within the last three (3) years.



PREFERRED JOB-RELATED EXPERIENCE:  One (1) year of hospital histology experience within the last three (3) years (routine staining required, must be able to cut large volumes of slides in allotted time).



Go to www.lgheath.org to apply!
This email was sent securely from the LGHealth Email Service

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This e-mail message, including any attachments, is for the sole use
of intended recipient(s) and may contain confidential and
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Any unauthorized review, use, disclosure or distribution is
prohibited.  
If you are not the intended recipient, please contact the sender by
reply e-mail and destroy all copies of the original message.

From cjbulmer <@t> sbcglobal.net  Tue Nov 30 12:25:53 2010
From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer)
Date: Tue Nov 30 12:25:57 2010
Subject: [Histonet] c-erbB2
Message-ID: <95927.80782.qm@web82303.mail.mud.yahoo.com>

Hello Histoland,
?
Has anyone seen any "B-9 ducts" staining positive with the c-erbB2 Oncoprotein
(Rabbit, conc.) # A0485 from Dako?
?
Thank you,
Cindy

Cynthia Bulmer HT(ASCP)QIHC
IHC Supervisor, CTPL
Waco, TX
From kc <@t> ka-recruiting.com  Tue Nov 30 12:33:50 2010
From: kc <@t> ka-recruiting.com (K.C. Carpenter)
Date: Tue Nov 30 12:33:26 2010
Subject: [Histonet] Are you looking to start the new year with a new job?
Message-ID: <2117949689.1291142030702.JavaMail.cfservice@SL4APP1>








Dear Histonet Community,

 

I hope you all had a Happy Thanksgiving and were able to enjoy some time off. I am a one of the founders of a healthcare recruiting firm.  I help Lab Professionals find permanent employment and I wanted to see if you are interested in exploring other career opportunities?  We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Histology Supervisor position with a hospital outside of Columbus Ohio.

 

My client is a century old, 300+ bed hospital that is part of one of the most well respected hospital systems in Ohio They are looking to hire a 1st shift Histology Supervisor to oversee 10 FTE's (histotechs and cytotechs).  We're looking for someone with strong bench experience who also has a minimum of 1 years of prior supervisory experience.  For consideration you must be either HT or HTL (ASCP) certified and be knowledgeable of CAP and CLIA requirements.  My clients offers one of the best compensation & benefits package around including relocation assistance when necessary.   If you are interested in learning more about this position, please call or email me at kc@ka-recruiting.com





 

Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates.

 



Current Histology Openings: 

NH - Histology Supervisor

NH - Histotechnologist

NY - Upstate - Histotechnologist

NY - Long Island - Histology Lab Manager

NY - New York City - Histotechnologist - 3rd shift


MI - Histology Manager 


OK - Histology Supervisor

GA - Histology Supervisor

NV - Las Vegas - Histotechnologist - 3rd shift

TN - Histotechnologist



 






















If you're interested in any of these opportunities or are currently looking for employment then please reply with an updated resume and let me know when you're available to talk.  If this is not the right time for you to make a career change then please let me know if you can refer someone who is.  We offer a generous referral bonus for anyone you refer to us that we place into any position across the country.  

 


To learn more about job openings in other parts of the country please contact me or visit our website at www.ka-recruiting.com.  


















 

Sincerely,



























KC Carpenter



K.A. Recruiting, Inc.


10 Post Office Square, 8th Floor South


Boston, MA 02109


P: (617) 692-2949


F: (617) 507-8009



kc@ka-recruiting.com



www.ka-recruiting.com
  








From robinsoc <@t> mercyhealth.com  Tue Nov 30 13:30:22 2010
From: robinsoc <@t> mercyhealth.com (Cynthia Robinson)
Date: Tue Nov 30 13:30:33 2010
Subject: [Histonet] Her2neu testing in gastric cancer
Message-ID: <4CF4FC6E.59AC.00AF.0@mercyhealth.com>

We have oncologists ordering Her2neu on gastric cases since Herceptin has now been approved for this treatment. Is anyone using Ventana Pathway Her2neu antibody clone for gastric tumor? How did you do validation and what disclaimer are you using?  

Thanks for your help.


Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com



From PMonfils <@t> Lifespan.org  Tue Nov 30 14:00:39 2010
From: PMonfils <@t> Lifespan.org (Monfils, Paul)
Date: Tue Nov 30 14:01:15 2010
Subject: [Histonet] Vibratome sectioning of lung
Message-ID: <4EBFF65383B74D49995298C4976D1D5E0777D271@LSRIEXCH1.lsmaster.lifespan.org>

Does anyone have experience sectioning agarose-embedded lung sections on
the Vibratome?  I have cut sections of various tissues on the Vibratome,
but am having a very difficult time with mouse lung.  The agarose does
not seem to adhere to the tissue, and the blade simply pulls the tissue
out of the agarose block.  Any suggestions will be most appreciated.

From ddreesen <@t> sbcglobal.net  Tue Nov 30 14:09:37 2010
From: ddreesen <@t> sbcglobal.net (Debbie Dreesen)
Date: Tue Nov 30 14:09:40 2010
Subject: [Histonet] Looking for info on SF Bay area jobs
In-Reply-To: <201011301801.oAUI1Pxs031704@flpd243.prodigy.net>
Message-ID: <837462.37123.qm@web81004.mail.mud.yahoo.com>

Hi Allison,
?
Here's a link to a?handy website that might be helpful to you. It tells salary range by state and also lists some job openings. 
?
http://www.salarylist.com/jobs-salary-by-states.htm

Debbie Dreesen, HT(ASCP)
From gupathlab <@t> gmail.com  Tue Nov 30 15:23:01 2010
From: gupathlab <@t> gmail.com (lab operations)
Date: Tue Nov 30 15:23:05 2010
Subject: [Histonet] Pathology Lab Employment opportunity
Message-ID: 

Looking for experienced histology technicians and cytotechnologists in
Columbus, Ohio for new GU pathology laboratory. Excellent compensation with
great benefits. Experience with IHC, FISH, and GU pathology a plus. Send
resume or CV to careers@aksm.com.
From Rcartun <@t> harthosp.org  Tue Nov 30 15:37:00 2010
From: Rcartun <@t> harthosp.org (Richard Cartun)
Date: Tue Nov 30 15:37:11 2010
Subject: [Histonet] Her2neu testing in gastric cancer
In-Reply-To: <4CF4FC6E.59AC.00AF.0@mercyhealth.com>
References: <4CF4FC6E.59AC.00AF.0@mercyhealth.com>
Message-ID: <4CF5282B.7400.0077.1@harthosp.org>

I don't use Ventana's HER2 antibody, but I can provide you with the following information.  I have tested 9 gastric CAs for HER2 overexpression and only one so far has shown what I would call classic "3+" (Positive) immunoreactivity.  This case also showed HER2 gene amplification by FISH.  One other case that we called "Equivocal" was also "Equivocal" by FISH.  All others have been negative by IHC (and some by FISH).

My good friend (and HER2 expert), Dr. David Hicks, informed me recently that, in his experience, 11% of gastric CAs will show HER2 alterations.  Almost all of the HER2 positive tumors are proximal and show intestinal differentiation.

I would recommend using IHC for screening and then reflex any case with "Equivocal" (2+) or"Positive" (3+) immunoreactivity to FISH until you get enough cases for your validation.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Cynthia Robinson"  11/30/2010 2:30 PM >>>
We have oncologists ordering Her2neu on gastric cases since Herceptin has now been approved for this treatment. Is anyone using Ventana Pathway Her2neu antibody clone for gastric tumor? How did you do validation and what disclaimer are you using?  

Thanks for your help.


Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com 



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From mbrooks <@t> incytepathology.com  Tue Nov 30 18:37:27 2010
From: mbrooks <@t> incytepathology.com (Matt Brooks)
Date: Tue Nov 30 18:37:32 2010
Subject: [Histonet] IHC protocols
Message-ID: <706224670091FE47997AEF88EFADE7CA0194EAF7@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>

Hello All,

Is there anyone who is using Ventana's BenchMark Ultra and/or XT and is
successfully running DBA.44, TIA-1, GCET-1, and/or FOXP-1?  If so would
you be willing to share your staining protocols with me on any of these
antibodies.

Thank you, 

Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
mbrooks@incytepathology.com
509-892-2744