[Histonet] weekend fixation

Alexandra Meinl alexandra.meinl <@t> gmail.com
Thu May 20 16:03:21 CDT 2010


We fix all specimens in NBF for 24h, wash them in tap water and store them
in alcohol 70% at 4°C until further processing. That works perfectly well
for us, and we never noticed any loss of staining.
But I also knew that some colleagues prefer to run a weekend program on
their Tissue Tek without delay so that the specimens stay infiltrated with
paraffin until Monday morning.

Alexandra



************************************************
Dr. Alexandra Meinl
Ludwig Boltzmann Institute
for Experimental and Clinical Traumatology
Austrian Cluster for Tissue Regeneration
Histology
Donaueschingenstrasse 13
1200 Vienna - Austria


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