[Histonet] Freezing spray artifact

[James L Burchette]

Erin - 

Try this trick when using the freezing spray: take a folded kim wipe 
tissue and fold it again, then again then the long fold is folded twice so 
you end up with roughly a 1 inch by 1 inch multi layer slide wiper. Run 
your finger down each crease and after the final fold, give it a little 
pinch and twist so it holds its shape. You are probably wondering what 
does this have to do with freezing spray artifact... Dip the square of Kim 
Wipe in your water bath and press it on the cut surface of your tissue 
block. It will act as an insulator when you spray the block surface. They 
are also good for soaking the face of a block and are better than a wad of 
tissue when wiping slides.

JB





"Martin, Erin" <Erin.Martin <@t> ucsf.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/19/2010 12:41 PM

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[Histonet] Freezing spray artifact






Has anyone run into a problem or artifact from freezing spray?  I think we 
may be having a problem with it but I can't find any pictures or 
descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

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