From irena.kirbis <@t> hotmail.com Sat May 1 04:16:27 2010 From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS) Date: Sat May 1 04:16:32 2010 Subject: [Histonet] Re: How to troubleshoot a cytology slide with dehydration problems In-Reply-To: References: , Message-ID: Dear Valerie, I can only agree that Papanicolaou stain is very tricky, having 20 yrs experiencies with it! looks very simple but you need to know some basic rules. first of all the slides should be hydrated at the beginning of staining procedure since the hematoxylin is water based stain! it seems to me thatyour slides are not enough hydrated, I never heard about sodium chloride solution as a hydrating but I guess cannot harm, however then you put slides in alcohol it's a bit confusing! and 25 seconds is a very short time for any incubation! beginning of a staining procedure depends on a fixation of the slides! in a case of a wet fixed slides you can proceed directly to staining (95% alcohol) if the slides are wet fixed and then dried you need to rehydrate them before staining and if you have spray fixed slides you need to remove the wax from slides before staining! my suggestions: 1. - leave slides in a sodium chloride longer, perhaps 2-3 minutes - alcohol 95% - alcohol 70% - water at least 3 min better 5 minutes, you'll probably need to shorten staining time in hematoxylin to 1 minute because the slides will be better hydrated and ready to accept stain. - staining time in EA solution is too short to allow proper staining and this is probably the reason to get reddish staining, you should leave the slides in EA 5 minutes since only then the light green will start to stain. hope this help! do you stain by hand or in a stainer? how often do you change stains and other solutions? you should also be very carefull to have the proper level of all solutions in a dishes! the basic rules is to keep the level of stains bellow the level of rinsing solutions. let me know about your staining results or even better to send me a picture! sometimes you can get the all redish staining also becouse of improper fixation! you can prepare your proprely fixed slide and stain it to see the results! best regards! Irena > From: vrodriguez10@gmail.com > Date: Fri, 30 Apr 2010 17:48:58 -0700 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: How to troubleshoot a cytology slide with dehydration problems > > I forgot to include the complete protocol that my lab uses so you can help > me better: > > 1.After dehydrating in sodium chloride for 25 seconds you have to dip the > slides in alcohol 95. > 2. 10 dips in water then 1.30 minutes in hematoxylin, it used to be 1 min, > then rinse in water. > 3. 1 min in rinse solution > 4.10 dips in water. > 5. 1 min in bluing solution > 6. 10 dips in water. > 7. 10 dips in alcohol. > 8. 10 dips in alcohol. > 9. 1 min in orange solution > 10. 15 dips in 95 alcohol. > 11. 15 dips in 95 alcohol. > 12. 3.15 mins in EA solution > 13. The slides are then dipped in 3 containers of 95 alcohol (20 dips each) > 14. Slides are dipped in 3 containers of 99 alcohol (15 dips in each) > 15. Then they are dipped in Isoxylene which is a combination of xylene and > 99 alcohol (15 dips) > 16. And lastly Xylene 10 dips > > > When the slides do not absorb the h20 saline seems that the slides absorb > more EA than hematoxylin. I don't know why. > > On Fri, Apr 30, 2010 at 5:34 PM, Valerie R. wrote: > > > I am an HTL but I have to stain pap smears. In the Papanicalou procedure > > for fine needle aspirations I have to stain the slides in sodium chloride > > first for 25 seconds in total (15 seconds in one container and 10 in another > > container). This for me is the trickiest part when staining cytology because > > if slides are not well dehydrated in saline then the cells are not going to > > absorb the stains well, and the result will be a pinkish slide, when it is > > suppose to look blue-greenish. > > > > I had this problem today where I stained an entire rack of slides, and all > > the slides turned blue (which means they stained correctly) except 4 slides > > which turned out pinkish (which is a sign that they did not dehydrated > > properly in sodium chloride (h20 saline). > > > > Is there a solution to this issue. Can these slides be re-stained? > > > > I know this newsgroup is for histology only but I would like to know if > > histotech and cytotechs have encountered this issue in their labs and how > > they ended up solving the problem. > > > > Thanks in advance > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: zmogljiva brezpla?na e-po?tna storitev z Microsoftovo za??ito https://signup.live.com/signup.aspx?id=60969 From alineumann <@t> aol.com Sat May 1 11:41:03 2010 From: alineumann <@t> aol.com (alineumann@aol.com) Date: Sat May 1 11:41:40 2010 Subject: [Histonet] Does anyone need books? Message-ID: <8CCB76438FB2942-1C08-14475@webmail-m084.sysops.aol.com> Hello all, I have some older microbiology, cytology, electron microscopy and immunohistochemical books, as well as a 1985 Internal Medicine text, would anyone like them for an underserved area? Thanks, Alice Neumann MD Western Wyoming Pathology 2001 Corner Creek Lane #67 Jackson, WY 83001 Cell: 307-413-4092 Home: 307-734-4410 From Bill.Tench <@t> pph.org Sat May 1 12:37:02 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Sat May 1 12:37:09 2010 Subject: [Histonet] Revalidation In-Reply-To: <20100501170049.2BCD264ED2@mail1.pph.org> References: <20100501170049.2BCD264ED2@mail1.pph.org> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863034@MAIL1.pph.local> Kevin, trust your judgment. You have made significant changes in your protocol. You need to revalidate. (and frankly, most changes in a standard protocol would require revalidation). Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From pathmaster <@t> yahoo.com Sat May 1 13:43:23 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Sat May 1 13:43:27 2010 Subject: [Histonet] RE-hydrating air dried slides for Pap stain Message-ID: <789166.47922.qm@web111111.mail.gq1.yahoo.com> Hi Valerie, It sounds like you are performing a Pap stain using saline baths to RE-hydrate air dried smears prepared at the bedside, I assume for? staining with Diff Quik stains. The rehydration ( not dehydration) takes place in the sodium chloride baths and restores the morphology of the cells to something closer to a wet fixed state. This takes place in 30 seconds.? You then must actually fix the? now rehydrated cells in 95% alcohol. This step should be of sufficient time to completely fix the cells as one would from the start, up to 20 minutes is needed. . Next the cells need to be rehydrated again, just placed in a water rinse, before staining with hematoxylin and proceeding with the Pap cytoplasmic stains. Awareness of these steps should obviate any problems. Finally, I wouldn't judge the success or failure of the stain by the color of the slide since different tissue fluid backgrounds and amounts of lysed blood might stain differently.? Check the slide microscoipically to assess the stain. Jeff Silverman From mikoff <@t> pacbell.net Sat May 1 14:05:58 2010 From: mikoff <@t> pacbell.net (Keith) Date: Sat May 1 14:06:01 2010 Subject: [Histonet] RE: H. D., Vol 78, Issue 1, Message: 7 In-Reply-To: <201005011703.o41H3ImL019405@flpd239.prodigy.net> Message-ID: Here's a copy of a procedure that provided consistently adequate results from my old one-man lab histo days... For your case, perhaps the slides in question were fixed differently or improperly(?) I would pull the questionable slides, delicately rehydrate them, run a control slide, and reprocess them with the following procedure. In this procedure, the staining agents, dehydrants, and clearing agents and can be modified to your facility and reagent list. Keith M. Mikoff, HTL/HT (ASCP) PAPANICOLAOU STAIN (PAP) PURPOSE: The following staining procedure is to be performed only on spray fixed slides. It is a routine stain used for any cytol- ogy (i.e., PAP smears, FNA procedures, or specimens of a fluid nature where cytology is required). PROCEDURE: 1. Dehydrate slides in 2 changes of isopropyl alcohol, 5 minutes each. 2. Rinse in tap water for two minutes. 3. Stain in Harris' Hematoxylin for 2 minutes. 4. Rinse in fresh tap water for 3 minutes. 5. Place in ammonia water for 1 minute. 6. Rinse in fresh tap water for 1 minute. 7. Dehydrate in 3 changes of Isopropyl alcohol, 2 minutes each. 8. Stain in O-G 6 for one minute. 9. Dehydrate in Isopropyl alcohol for 2 changes, 2 minutes each. 10. Stain in EA-50 for 4 minutes. 11. Place in Isopropyl alcohol, 3 changes, at 1 1/2 minutes each 12. To clear the slides, place in 3 changes of Histoclear, for 2 minutes each, and coverslip. From histotech <@t> imagesbyhopper.com Sun May 2 08:01:04 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sun May 2 08:01:10 2010 Subject: [Histonet] disposable blade holder In-Reply-To: <6746115DFA761840A605BB27624424AAEF0F10D5@USPHO-MXVS01.amer.thermo.com> Message-ID: <1729E60B0B6D456BB67E686C815CC071@hopperPC> As a comical sidelight to the last sentence that Tyler wrote ... I have a pathologist who was very upset that our cryostat didn't seem to be working properly. I rushed up to the frozen room to assist and he said, "well, nevermind, I got a slide and diagnosis." I looked in the cryostat and said to him "you actually got a section and were able to render a diagnosis?" "yes" he said. "WOW!, you must be the ONLY pathologist who is capable of sectioning tissue without a knife ..." ;o) He sectioned the tissue and truly had no idea there wasn't a knife in the blade holder! :o) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liebig, Tyler K. Sent: Friday, April 30, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposable blade holder Hello Lucy, In regards to your trouble finding an HM310 blade holder we do have one in our refurbished pool that I could have sent to you. It is used but in working order so we can let you have it at no charge. If you let me know where to ship it I will have it sent. Hope that helps, It's hard to cut without a blade holder. Regards, Tyler Liebig Product Manager Histology Instrumentation and IHC Anatomical Pathology Thermo Fisher Scientific 46360 Fremont Blvd Fremont, CA 94538 USA Office: (510) 979-5000 Mobile: (510) 299-1751 Fax: (510) 979-5239 tyler.liebig@thermofisher.com www.thermo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2845 - Release Date: 04/30/10 11:34:00 From histotech <@t> imagesbyhopper.com Sun May 2 08:04:16 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sun May 2 08:04:20 2010 Subject: [Histonet] Slide and Block Storage In-Reply-To: <2206BD35B2444BBA8DBF793EF14858F9@dlne.local> Message-ID: <7180DF002734414D8FEEE9A4A4600940@hopperPC> In our facility, we maintain both the blocks and slides. We also store older blocks/slides in a climate controled an off-site facility. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 11:00 AM To: 'Nails, Felton'; 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage Clients fax orders for deepers and special stains which we perform and send them out with their next package for them to read and report. -----Original Message----- From: Nails, Felton [mailto:flnails@texaschildrens.org] Sent: Friday, April 30, 2010 10:48 AM To: 'Elizabeth Kronenberger'; 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage So what if the reporting company need to order special stains, it adds too much time having to request the blocks or additional unstained slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 9:40 AM To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage If our lab does slide preps for other clients (Technical component only), we hold the blocks at our facility. The slides are read and reported by the client, they hold the slides. Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Friday, April 30, 2010 12:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2845 - Release Date: 04/30/10 11:34:00 From thisisann <@t> aol.com Sun May 2 17:10:41 2010 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Sun May 2 17:11:01 2010 Subject: [Histonet] Full Time 4-day, 40 hr position in NJ Message-ID: <8CCB85B6FE86629-117C-1CE18@webmail-d028.sysops.aol.com> We are a reference lab in central NJ looking for a 'seasoned' Histotech to work four 10-hr days, Tues - Friday, 8am -6:30pm in our very busy Histology laboratory. Job duties include: Grossing, embedding, cutting, staining (Ventana platform for both H&E and IHC) coverslipping, etc,.) Experienced Techs Only. Please e-mail your resume to Thisisann@aol.com if you are interested. From flnails <@t> texaschildrens.org Sun May 2 18:50:30 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Sun May 2 18:52:45 2010 Subject: [Histonet] RE: [Derm procedure Message-ID: Would anyone out there in a Derm Lab be willing to share their procedure used to process tissue? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com [thisisann@aol.com] Sent: Sunday, May 02, 2010 5:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Full Time 4-day, 40 hr position in NJ We are a reference lab in central NJ looking for a 'seasoned' Histotech to work four 10-hr days, Tues - Friday, 8am -6:30pm in our very busy Histology laboratory. Job duties include: Grossing, embedding, cutting, staining (Ventana platform for both H&E and IHC) coverslipping, etc,.) Experienced Techs Only. Please e-mail your resume to Thisisann@aol.com if you are interested. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From AnthonyH <@t> chw.edu.au Sun May 2 19:37:12 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun May 2 19:37:27 2010 Subject: [Histonet] Re: How to troubleshoot a cytology slide withdehydration problems In-Reply-To: Message-ID: Valerie, The technique you are using is the re-hydration technique of air-dried slides of Ng et al (Acta Cytolog 38(1):56-64, 1994)- 30 sec in saline followed by fixation in 95% ethanol (at this stage, slides can remain in ethanol before staining). Please note that it is a re-hydration, not a de-hydration step. I have found that slides that have been air-dried for longer than two days do not rehydrate well. Cells appear pink with large air-dried, pale staining nuclei - lacking chromatin details. Unfortunately there is little you can do in these cases. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Valerie R. Sent: Saturday, 1 May 2010 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: How to troubleshoot a cytology slide withdehydration problems I forgot to include the complete protocol that my lab uses so you can help me better: 1.After dehydrating in sodium chloride for 25 seconds you have to dip the slides in alcohol 95. 2. 10 dips in water then 1.30 minutes in hematoxylin, it used to be 1 min, then rinse in water. 3. 1 min in rinse solution 4.10 dips in water. 5. 1 min in bluing solution 6. 10 dips in water. 7. 10 dips in alcohol. 8. 10 dips in alcohol. 9. 1 min in orange solution 10. 15 dips in 95 alcohol. 11. 15 dips in 95 alcohol. 12. 3.15 mins in EA solution 13. The slides are then dipped in 3 containers of 95 alcohol (20 dips each) 14. Slides are dipped in 3 containers of 99 alcohol (15 dips in each) 15. Then they are dipped in Isoxylene which is a combination of xylene and 99 alcohol (15 dips) 16. And lastly Xylene 10 dips When the slides do not absorb the h20 saline seems that the slides absorb more EA than hematoxylin. I don't know why. On Fri, Apr 30, 2010 at 5:34 PM, Valerie R. wrote: > I am an HTL but I have to stain pap smears. In the Papanicalou > procedure for fine needle aspirations I have to stain the slides in > sodium chloride first for 25 seconds in total (15 seconds in one > container and 10 in another container). This for me is the trickiest > part when staining cytology because if slides are not well dehydrated > in saline then the cells are not going to absorb the stains well, and > the result will be a pinkish slide, when it is suppose to look > blue-greenish. > > I had this problem today where I stained an entire rack of slides, and > all the slides turned blue (which means they stained correctly) except > 4 slides which turned out pinkish (which is a sign that they did not > dehydrated properly in sodium chloride (h20 saline). > > Is there a solution to this issue. Can these slides be re-stained? > > I know this newsgroup is for histology only but I would like to know > if histotech and cytotechs have encountered this issue in their labs > and how they ended up solving the problem. > > Thanks in advance > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From cbarone <@t> NEMOURS.ORG Sun May 2 19:44:53 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Sun May 2 19:44:56 2010 Subject: [Histonet] Position in Northern Delaware Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7C9B@wlmmsx01.nemours.org> Seeking HT( ASCP) or HTLASCP) for position 50% clinical and 50% research. Must have antibody development and some ? in situ experience (manual): send salary request and CV to Nemours- AI duPont Hosp. for children HR: care of Terry Timmons: ttimmons@nemours.org From cbarone <@t> NEMOURS.ORG Sun May 2 19:46:32 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Sun May 2 19:46:37 2010 Subject: [Histonet] RE: Histonet Digest, Vol 78, Issue 1 In-Reply-To: References: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7C9C@wlmmsx01.nemours.org> I never throw a book away! cbarone@nemours.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, May 01, 2010 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD68 (Dana Settembre) 2. Re: CD68 (Fabrice gankam) 3. Repeat validation when IHC protocol changed? (Kevin_Kurtz@ssmhc.com) 4. CD68??` (Jeffrey Silverman) 5. disposable blade holder (Liebig, Tyler K.) 6. How to troubleshoot a cytology slide with dehydration problems (Valerie R.) 7. Re: How to troubleshoot a cytology slide with dehydration problems (Valerie R.) 8. RE: Re: How to troubleshoot a cytology slide with dehydration problems (IRENA SREBOTNIK KIRBIS) 9. Does anyone need books? (alineumann@aol.com) ---------------------------------------------------------------------- Message: 1 Date: Fri, 30 Apr 2010 14:15:42 -0400 From: "Dana Settembre" Subject: RE: [Histonet] CD68 To: "Histonet" , "Drew Sally A" Message-ID: Content-Type: text/plain; charset=US-ASCII We are currently using KP-1 and HAM 56 Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Drew Sally A 04/30/10 12:11 PM >>> We also use KP1 Sally -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 30, 2010 10:49 AM To: 'Histonet' Subject: [Histonet] CD68 Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Fri, 30 Apr 2010 13:32:58 -0500 From: Fabrice gankam Subject: Re: [Histonet] CD68 To: Cynthia Pyse , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I use the ED1 from abdserotec and it works perfectly can dilute up to 1/500 to 1/1000 2010/4/30 Cynthia Pyse > Happy Friday Everyone > > What clone is everyone using for the CD68 antibody for FFPE human tissue? > Thanks for the info in advance. Everyone have a great weekend. > > > > Cindy Pyse, CLT, HT (ASCP) > > Histology Supervisor > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Fri, 30 Apr 2010 13:46:16 -0500 From: Kevin_Kurtz@ssmhc.com Subject: [Histonet] Repeat validation when IHC protocol changed? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Recently our IHC vendor sent a tech to help us out with some weak staining affecting multiple antibodies. Several staining protocols were changed, including adjusting the incubation time and adding amplification. The results were great in the slides that I was shown. The tech said that revalidation was unnecessary, since the changes to the protocol were considered "minor". However, I personally disagree, since we don't know how the change in the staining protocol could potentially affect staining of other tissues (especially tissues that would be expected to be negative). Before subjecting our lab to the cost and effort of revalidation, I'd like to get your opinion. For new antibodies, I usually validate using 10 cases expected to be positive and 10 cases expected to be negative for the antibody in question. For revalidation, I was thinking of running 5 positive cases and 5 negative cases. Thanks for your input - Kevin Kurtz, M.D. St. Mary's Hospital Madison, Wisconsin Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ------------------------------ Message: 4 Date: Fri, 30 Apr 2010 11:47:17 -0700 (PDT) From: Jeffrey Silverman Subject: [Histonet] CD68??` To: cpyse@x-celllab.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <821799.98032.qm@web111111.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii Another vote for KP-1. It also stains mast cells. Jeff Silverman ------------------------------ Message: 5 Date: Fri, 30 Apr 2010 12:40:45 -0700 From: "Liebig, Tyler K." Subject: [Histonet] disposable blade holder To: "histonet@lists.utsouthwestern.edu" Message-ID: <6746115DFA761840A605BB27624424AAEF0F10D5@USPHO-MXVS01.amer.thermo.com> Content-Type: text/plain; charset="us-ascii" Hello Lucy, In regards to your trouble finding an HM310 blade holder we do have one in our refurbished pool that I could have sent to you. It is used but in working order so we can let you have it at no charge. If you let me know where to ship it I will have it sent. Hope that helps, It's hard to cut without a blade holder. Regards, Tyler Liebig Product Manager Histology Instrumentation and IHC Anatomical Pathology Thermo Fisher Scientific 46360 Fremont Blvd Fremont, CA 94538 USA Office: (510) 979-5000 Mobile: (510) 299-1751 Fax: (510) 979-5239 tyler.liebig@thermofisher.com www.thermo.com ------------------------------ Message: 6 Date: Fri, 30 Apr 2010 17:34:39 -0700 From: "Valerie R." Subject: [Histonet] How to troubleshoot a cytology slide with dehydration problems To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I am an HTL but I have to stain pap smears. In the Papanicalou procedure for fine needle aspirations I have to stain the slides in sodium chloride first for 25 seconds in total (15 seconds in one container and 10 in another container). This for me is the trickiest part when staining cytology because if slides are not well dehydrated in saline then the cells are not going to absorb the stains well, and the result will be a pinkish slide, when it is suppose to look blue-greenish. I had this problem today where I stained an entire rack of slides, and all the slides turned blue (which means they stained correctly) except 4 slides which turned out pinkish (which is a sign that they did not dehydrated properly in sodium chloride (h20 saline). Is there a solution to this issue. Can these slides be re-stained? I know this newsgroup is for histology only but I would like to know if histotech and cytotechs have encountered this issue in their labs and how they ended up solving the problem. Thanks in advance ------------------------------ Message: 7 Date: Fri, 30 Apr 2010 17:48:58 -0700 From: "Valerie R." Subject: [Histonet] Re: How to troubleshoot a cytology slide with dehydration problems To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I forgot to include the complete protocol that my lab uses so you can help me better: 1.After dehydrating in sodium chloride for 25 seconds you have to dip the slides in alcohol 95. 2. 10 dips in water then 1.30 minutes in hematoxylin, it used to be 1 min, then rinse in water. 3. 1 min in rinse solution 4.10 dips in water. 5. 1 min in bluing solution 6. 10 dips in water. 7. 10 dips in alcohol. 8. 10 dips in alcohol. 9. 1 min in orange solution 10. 15 dips in 95 alcohol. 11. 15 dips in 95 alcohol. 12. 3.15 mins in EA solution 13. The slides are then dipped in 3 containers of 95 alcohol (20 dips each) 14. Slides are dipped in 3 containers of 99 alcohol (15 dips in each) 15. Then they are dipped in Isoxylene which is a combination of xylene and 99 alcohol (15 dips) 16. And lastly Xylene 10 dips When the slides do not absorb the h20 saline seems that the slides absorb more EA than hematoxylin. I don't know why. On Fri, Apr 30, 2010 at 5:34 PM, Valerie R. wrote: > I am an HTL but I have to stain pap smears. In the Papanicalou procedure > for fine needle aspirations I have to stain the slides in sodium chloride > first for 25 seconds in total (15 seconds in one container and 10 in another > container). This for me is the trickiest part when staining cytology because > if slides are not well dehydrated in saline then the cells are not going to > absorb the stains well, and the result will be a pinkish slide, when it is > suppose to look blue-greenish. > > I had this problem today where I stained an entire rack of slides, and all > the slides turned blue (which means they stained correctly) except 4 slides > which turned out pinkish (which is a sign that they did not dehydrated > properly in sodium chloride (h20 saline). > > Is there a solution to this issue. Can these slides be re-stained? > > I know this newsgroup is for histology only but I would like to know if > histotech and cytotechs have encountered this issue in their labs and how > they ended up solving the problem. > > Thanks in advance > > > ------------------------------ Message: 8 Date: Sat, 1 May 2010 11:16:27 +0200 From: IRENA SREBOTNIK KIRBIS Subject: RE: [Histonet] Re: How to troubleshoot a cytology slide with dehydration problems To: Message-ID: Content-Type: text/plain; charset="iso-8859-2" Dear Valerie, I can only agree that Papanicolaou stain is very tricky, having 20 yrs experiencies with it! looks very simple but you need to know some basic rules. first of all the slides should be hydrated at the beginning of staining procedure since the hematoxylin is water based stain! it seems to me thatyour slides are not enough hydrated, I never heard about sodium chloride solution as a hydrating but I guess cannot harm, however then you put slides in alcohol it's a bit confusing! and 25 seconds is a very short time for any incubation! beginning of a staining procedure depends on a fixation of the slides! in a case of a wet fixed slides you can proceed directly to staining (95% alcohol) if the slides are wet fixed and then dried you need to rehydrate them before staining and if you have spray fixed slides you need to remove the wax from slides before staining! my suggestions: 1. - leave slides in a sodium chloride longer, perhaps 2-3 minutes - alcohol 95% - alcohol 70% - water at least 3 min better 5 minutes, you'll probably need to shorten staining time in hematoxylin to 1 minute because the slides will be better hydrated and ready to accept stain. - staining time in EA solution is too short to allow proper staining and this is probably the reason to get reddish staining, you should leave the slides in EA 5 minutes since only then the light green will start to stain. hope this help! do you stain by hand or in a stainer? how often do you change stains and other solutions? you should also be very carefull to have the proper level of all solutions in a dishes! the basic rules is to keep the level of stains bellow the level of rinsing solutions. let me know about your staining results or even better to send me a picture! sometimes you can get the all redish staining also becouse of improper fixation! you can prepare your proprely fixed slide and stain it to see the results! best regards! Irena > From: vrodriguez10@gmail.com > Date: Fri, 30 Apr 2010 17:48:58 -0700 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: How to troubleshoot a cytology slide with dehydration problems > > I forgot to include the complete protocol that my lab uses so you can help > me better: > > 1.After dehydrating in sodium chloride for 25 seconds you have to dip the > slides in alcohol 95. > 2. 10 dips in water then 1.30 minutes in hematoxylin, it used to be 1 min, > then rinse in water. > 3. 1 min in rinse solution > 4.10 dips in water. > 5. 1 min in bluing solution > 6. 10 dips in water. > 7. 10 dips in alcohol. > 8. 10 dips in alcohol. > 9. 1 min in orange solution > 10. 15 dips in 95 alcohol. > 11. 15 dips in 95 alcohol. > 12. 3.15 mins in EA solution > 13. The slides are then dipped in 3 containers of 95 alcohol (20 dips each) > 14. Slides are dipped in 3 containers of 99 alcohol (15 dips in each) > 15. Then they are dipped in Isoxylene which is a combination of xylene and > 99 alcohol (15 dips) > 16. And lastly Xylene 10 dips > > > When the slides do not absorb the h20 saline seems that the slides absorb > more EA than hematoxylin. I don't know why. > > On Fri, Apr 30, 2010 at 5:34 PM, Valerie R. wrote: > > > I am an HTL but I have to stain pap smears. In the Papanicalou procedure > > for fine needle aspirations I have to stain the slides in sodium chloride > > first for 25 seconds in total (15 seconds in one container and 10 in another > > container). This for me is the trickiest part when staining cytology because > > if slides are not well dehydrated in saline then the cells are not going to > > absorb the stains well, and the result will be a pinkish slide, when it is > > suppose to look blue-greenish. > > > > I had this problem today where I stained an entire rack of slides, and all > > the slides turned blue (which means they stained correctly) except 4 slides > > which turned out pinkish (which is a sign that they did not dehydrated > > properly in sodium chloride (h20 saline). > > > > Is there a solution to this issue. Can these slides be re-stained? > > > > I know this newsgroup is for histology only but I would like to know if > > histotech and cytotechs have encountered this issue in their labs and how > > they ended up solving the problem. > > > > Thanks in advance > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: zmogljiva brezpla?na e-po?tna storitev z Microsoftovo za??ito https://signup.live.com/signup.aspx?id=60969 ------------------------------ Message: 9 Date: Sat, 01 May 2010 12:41:03 -0400 From: alineumann@aol.com Subject: [Histonet] Does anyone need books? To: histonet@pathology.swmed.edu, members@lists.cytopathology.org, soamail@comcast.net Message-ID: <8CCB76438FB2942-1C08-14475@webmail-m084.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello all, I have some older microbiology, cytology, electron microscopy and immunohistochemical books, as well as a 1985 Internal Medicine text, would anyone like them for an underserved area? Thanks, Alice Neumann MD Western Wyoming Pathology 2001 Corner Creek Lane #67 Jackson, WY 83001 Cell: 307-413-4092 Home: 307-734-4410 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 1 *************************************** From GDawson <@t> dynacaremilwaukee.com Mon May 3 07:21:18 2010 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon May 3 07:21:22 2010 Subject: [Histonet] CD68 In-Reply-To: <000301cae87c$a33b6d50$e9b247f0$@com> Message-ID: I use both KP-1 and PGM-1. KP-1 is ordered about ten times as much as the PGM-1. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 30, 2010 10:49 AM To: 'Histonet' Subject: [Histonet] CD68 Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tyler.liebig <@t> thermofisher.com Mon May 3 07:32:20 2010 From: tyler.liebig <@t> thermofisher.com (Liebig, Tyler K.) Date: Mon May 3 07:32:22 2010 Subject: [Histonet] RE: Histonet Digest, Vol 78, Issue 2 In-Reply-To: <20100502170340.446E4EBC001@usmx03.thermofisher.com> References: <20100502170340.446E4EBC001@usmx03.thermofisher.com> Message-ID: <6746115DFA761840A605BB27624424AAEF0F1236@USPHO-MXVS01.amer.thermo.com> That is a funny story, I would like to see the slide that was cut from a "blade holder section" with no blade in the clamp. I have heard it can be done but never see it myself. Though in old blade holders with loose clamps I have seen users get two blades in there at once. That didn't section to well either. Tyler -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, May 02, 2010 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Revalidation (Tench, Bill) 2. RE-hydrating air dried slides for Pap stain (Jeffrey Silverman) 3. RE: H. D., Vol 78, Issue 1, Message: 7 (Keith) 4. RE: disposable blade holder (histotech@imagesbyhopper.com) 5. RE: Slide and Block Storage (histotech@imagesbyhopper.com) ---------------------------------------------------------------------- Message: 1 Date: Sat, 1 May 2010 10:37:02 -0700 From: "Tench, Bill" Subject: [Histonet] Revalidation To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863034@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii Kevin, trust your judgment. You have made significant changes in your protocol. You need to revalidate. (and frankly, most changes in a standard protocol would require revalidation). Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 2 Date: Sat, 1 May 2010 11:43:23 -0700 (PDT) From: Jeffrey Silverman Subject: [Histonet] RE-hydrating air dried slides for Pap stain To: vrodriguez10@gmail.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <789166.47922.qm@web111111.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Valerie, It sounds like you are performing a Pap stain using saline baths to RE-hydrate air dried smears prepared at the bedside, I assume for? staining with Diff Quik stains. The rehydration ( not dehydration) takes place in the sodium chloride baths and restores the morphology of the cells to something closer to a wet fixed state. This takes place in 30 seconds.? You then must actually fix the? now rehydrated cells in 95% alcohol. This step should be of sufficient time to completely fix the cells as one would from the start, up to 20 minutes is needed. . Next the cells need to be rehydrated again, just placed in a water rinse, before staining with hematoxylin and proceeding with the Pap cytoplasmic stains. Awareness of these steps should obviate any problems. Finally, I wouldn't judge the success or failure of the stain by the color of the slide since different tissue fluid backgrounds and amounts of lysed blood might stain differently.? Check the slide microscoipically to assess the stain. Jeff Silverman ------------------------------ Message: 3 Date: Sat, 1 May 2010 12:05:58 -0700 From: "Keith" Subject: [Histonet] RE: H. D., Vol 78, Issue 1, Message: 7 To: Message-ID: Content-Type: text/plain; charset="us-ascii" Here's a copy of a procedure that provided consistently adequate results from my old one-man lab histo days... For your case, perhaps the slides in question were fixed differently or improperly(?) I would pull the questionable slides, delicately rehydrate them, run a control slide, and reprocess them with the following procedure. In this procedure, the staining agents, dehydrants, and clearing agents and can be modified to your facility and reagent list. Keith M. Mikoff, HTL/HT (ASCP) PAPANICOLAOU STAIN (PAP) PURPOSE: The following staining procedure is to be performed only on spray fixed slides. It is a routine stain used for any cytol- ogy (i.e., PAP smears, FNA procedures, or specimens of a fluid nature where cytology is required). PROCEDURE: 1. Dehydrate slides in 2 changes of isopropyl alcohol, 5 minutes each. 2. Rinse in tap water for two minutes. 3. Stain in Harris' Hematoxylin for 2 minutes. 4. Rinse in fresh tap water for 3 minutes. 5. Place in ammonia water for 1 minute. 6. Rinse in fresh tap water for 1 minute. 7. Dehydrate in 3 changes of Isopropyl alcohol, 2 minutes each. 8. Stain in O-G 6 for one minute. 9. Dehydrate in Isopropyl alcohol for 2 changes, 2 minutes each. 10. Stain in EA-50 for 4 minutes. 11. Place in Isopropyl alcohol, 3 changes, at 1 1/2 minutes each 12. To clear the slides, place in 3 changes of Histoclear, for 2 minutes each, and coverslip. ------------------------------ Message: 4 Date: Sun, 2 May 2010 09:01:04 -0400 From: Subject: RE: [Histonet] disposable blade holder To: Message-ID: <1729E60B0B6D456BB67E686C815CC071@hopperPC> Content-Type: text/plain; charset="US-ASCII" As a comical sidelight to the last sentence that Tyler wrote ... I have a pathologist who was very upset that our cryostat didn't seem to be working properly. I rushed up to the frozen room to assist and he said, "well, nevermind, I got a slide and diagnosis." I looked in the cryostat and said to him "you actually got a section and were able to render a diagnosis?" "yes" he said. "WOW!, you must be the ONLY pathologist who is capable of sectioning tissue without a knife ..." ;o) He sectioned the tissue and truly had no idea there wasn't a knife in the blade holder! :o) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liebig, Tyler K. Sent: Friday, April 30, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposable blade holder Hello Lucy, In regards to your trouble finding an HM310 blade holder we do have one in our refurbished pool that I could have sent to you. It is used but in working order so we can let you have it at no charge. If you let me know where to ship it I will have it sent. Hope that helps, It's hard to cut without a blade holder. Regards, Tyler Liebig Product Manager Histology Instrumentation and IHC Anatomical Pathology Thermo Fisher Scientific 46360 Fremont Blvd Fremont, CA 94538 USA Office: (510) 979-5000 Mobile: (510) 299-1751 Fax: (510) 979-5239 tyler.liebig@thermofisher.com www.thermo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2845 - Release Date: 04/30/10 11:34:00 ------------------------------ Message: 5 Date: Sun, 2 May 2010 09:04:16 -0400 From: Subject: RE: [Histonet] Slide and Block Storage To: Message-ID: <7180DF002734414D8FEEE9A4A4600940@hopperPC> Content-Type: text/plain; charset="US-ASCII" In our facility, we maintain both the blocks and slides. We also store older blocks/slides in a climate controled an off-site facility. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 11:00 AM To: 'Nails, Felton'; 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage Clients fax orders for deepers and special stains which we perform and send them out with their next package for them to read and report. -----Original Message----- From: Nails, Felton [mailto:flnails@texaschildrens.org] Sent: Friday, April 30, 2010 10:48 AM To: 'Elizabeth Kronenberger'; 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage So what if the reporting company need to order special stains, it adds too much time having to request the blocks or additional unstained slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 9:40 AM To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage If our lab does slide preps for other clients (Technical component only), we hold the blocks at our facility. The slides are read and reported by the client, they hold the slides. Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Friday, April 30, 2010 12:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2845 - Release Date: 04/30/10 11:34:00 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 2 *************************************** From mpence <@t> grhs.net Mon May 3 08:18:39 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Mon May 3 08:18:44 2010 Subject: [Histonet] RE: Histonet Digest, Vol 78, Issue 2 In-Reply-To: <6746115DFA761840A605BB27624424AAEF0F1236@USPHO-MXVS01.amer.thermo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DF4@is-e2k3.grhs.net> I had one of my techs come to me one day saying that she thought I needed to look at her microtome because she had not been able to cut anything on her microtome today. She thought something had gone wrong with the tissue during processing. The other techs though were sectioning fine. This tech has been with me for over 20 years. I went over and looked at her microtome and told her that it might improve if she tried a blade in the blade holder to start. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liebig, Tyler K. Sent: Monday, May 03, 2010 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 78, Issue 2 That is a funny story, I would like to see the slide that was cut from a "blade holder section" with no blade in the clamp. I have heard it can be done but never see it myself. Though in old blade holders with loose clamps I have seen users get two blades in there at once. That didn't section to well either. Tyler -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, May 02, 2010 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Revalidation (Tench, Bill) 2. RE-hydrating air dried slides for Pap stain (Jeffrey Silverman) 3. RE: H. D., Vol 78, Issue 1, Message: 7 (Keith) 4. RE: disposable blade holder (histotech@imagesbyhopper.com) 5. RE: Slide and Block Storage (histotech@imagesbyhopper.com) ---------------------------------------------------------------------- Message: 1 Date: Sat, 1 May 2010 10:37:02 -0700 From: "Tench, Bill" Subject: [Histonet] Revalidation To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863034@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii Kevin, trust your judgment. You have made significant changes in your protocol. You need to revalidate. (and frankly, most changes in a standard protocol would require revalidation). Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 2 Date: Sat, 1 May 2010 11:43:23 -0700 (PDT) From: Jeffrey Silverman Subject: [Histonet] RE-hydrating air dried slides for Pap stain To: vrodriguez10@gmail.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <789166.47922.qm@web111111.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Valerie, It sounds like you are performing a Pap stain using saline baths to RE-hydrate air dried smears prepared at the bedside, I assume for? staining with Diff Quik stains. The rehydration ( not dehydration) takes place in the sodium chloride baths and restores the morphology of the cells to something closer to a wet fixed state. This takes place in 30 seconds.? You then must actually fix the? now rehydrated cells in 95% alcohol. This step should be of sufficient time to completely fix the cells as one would from the start, up to 20 minutes is needed. . Next the cells need to be rehydrated again, just placed in a water rinse, before staining with hematoxylin and proceeding with the Pap cytoplasmic stains. Awareness of these steps should obviate any problems. Finally, I wouldn't judge the success or failure of the stain by the color of the slide since different tissue fluid backgrounds and amounts of lysed blood might stain differently.? Check the slide microscoipically to assess the stain. Jeff Silverman ------------------------------ Message: 3 Date: Sat, 1 May 2010 12:05:58 -0700 From: "Keith" Subject: [Histonet] RE: H. D., Vol 78, Issue 1, Message: 7 To: Message-ID: Content-Type: text/plain; charset="us-ascii" Here's a copy of a procedure that provided consistently adequate results from my old one-man lab histo days... For your case, perhaps the slides in question were fixed differently or improperly(?) I would pull the questionable slides, delicately rehydrate them, run a control slide, and reprocess them with the following procedure. In this procedure, the staining agents, dehydrants, and clearing agents and can be modified to your facility and reagent list. Keith M. Mikoff, HTL/HT (ASCP) PAPANICOLAOU STAIN (PAP) PURPOSE: The following staining procedure is to be performed only on spray fixed slides. It is a routine stain used for any cytol- ogy (i.e., PAP smears, FNA procedures, or specimens of a fluid nature where cytology is required). PROCEDURE: 1. Dehydrate slides in 2 changes of isopropyl alcohol, 5 minutes each. 2. Rinse in tap water for two minutes. 3. Stain in Harris' Hematoxylin for 2 minutes. 4. Rinse in fresh tap water for 3 minutes. 5. Place in ammonia water for 1 minute. 6. Rinse in fresh tap water for 1 minute. 7. Dehydrate in 3 changes of Isopropyl alcohol, 2 minutes each. 8. Stain in O-G 6 for one minute. 9. Dehydrate in Isopropyl alcohol for 2 changes, 2 minutes each. 10. Stain in EA-50 for 4 minutes. 11. Place in Isopropyl alcohol, 3 changes, at 1 1/2 minutes each 12. To clear the slides, place in 3 changes of Histoclear, for 2 minutes each, and coverslip. ------------------------------ Message: 4 Date: Sun, 2 May 2010 09:01:04 -0400 From: Subject: RE: [Histonet] disposable blade holder To: Message-ID: <1729E60B0B6D456BB67E686C815CC071@hopperPC> Content-Type: text/plain; charset="US-ASCII" As a comical sidelight to the last sentence that Tyler wrote ... I have a pathologist who was very upset that our cryostat didn't seem to be working properly. I rushed up to the frozen room to assist and he said, "well, nevermind, I got a slide and diagnosis." I looked in the cryostat and said to him "you actually got a section and were able to render a diagnosis?" "yes" he said. "WOW!, you must be the ONLY pathologist who is capable of sectioning tissue without a knife ..." ;o) He sectioned the tissue and truly had no idea there wasn't a knife in the blade holder! :o) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liebig, Tyler K. Sent: Friday, April 30, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposable blade holder Hello Lucy, In regards to your trouble finding an HM310 blade holder we do have one in our refurbished pool that I could have sent to you. It is used but in working order so we can let you have it at no charge. If you let me know where to ship it I will have it sent. Hope that helps, It's hard to cut without a blade holder. Regards, Tyler Liebig Product Manager Histology Instrumentation and IHC Anatomical Pathology Thermo Fisher Scientific 46360 Fremont Blvd Fremont, CA 94538 USA Office: (510) 979-5000 Mobile: (510) 299-1751 Fax: (510) 979-5239 tyler.liebig@thermofisher.com www.thermo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2845 - Release Date: 04/30/10 11:34:00 ------------------------------ Message: 5 Date: Sun, 2 May 2010 09:04:16 -0400 From: Subject: RE: [Histonet] Slide and Block Storage To: Message-ID: <7180DF002734414D8FEEE9A4A4600940@hopperPC> Content-Type: text/plain; charset="US-ASCII" In our facility, we maintain both the blocks and slides. We also store older blocks/slides in a climate controled an off-site facility. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 11:00 AM To: 'Nails, Felton'; 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage Clients fax orders for deepers and special stains which we perform and send them out with their next package for them to read and report. -----Original Message----- From: Nails, Felton [mailto:flnails@texaschildrens.org] Sent: Friday, April 30, 2010 10:48 AM To: 'Elizabeth Kronenberger'; 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage So what if the reporting company need to order special stains, it adds too much time having to request the blocks or additional unstained slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 9:40 AM To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage If our lab does slide preps for other clients (Technical component only), we hold the blocks at our facility. The slides are read and reported by the client, they hold the slides. Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Friday, April 30, 2010 12:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ---- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================================================================== ==== == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2845 - Release Date: 04/30/10 11:34:00 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 2 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcdukes <@t> lexhealth.org Mon May 3 08:35:56 2010 From: bcdukes <@t> lexhealth.org (Blake Taylor) Date: Mon May 3 08:36:05 2010 Subject: [Histonet] Job Listing: Full time Position in West Columbia, SC Message-ID: Histologist Full-Time 3AM - 11:30 AM Basic Functions: Prepares slides from surgical, outpatient and bone marrow specimens for examination by a pathologist. Perform special stains both by hand and thru automation. Embeds tissues into molds after processing, selecting proper size base mold and correct orientation of tissue. Prepares a variety of solutions according to written protocols. Handles the general repair and maintenance of all equipment. Minimum Qualifications: Must be a graduate of an AMA approved program or possess 3-5 years of histology experience ASCP registered or registry eligibility Must have a discernible track record of a positive, can-do attitude Should have strong leadership ability and/or experience as a Lead Tech, or, previously in a charge role Four (4) or more years of current laboratory experience in histology, with exposure to quality assurance processes, client/patient interaction, etc. Preferred Qualifications: HT (ASCP) or equivalent or registered by a nationally recognized credentialing body Graduate of AMA approved Histology program 3+ years of histology experience Learn more about the position, our hospital system, employee benefits, or apply online by visiting our website at www.lexmed.com . Blake Taylor Section Supervisor Dept. of Pathology Lexington Medical Center 803-936-8214 bcdukes@lexhealth.org ______________________________________________________________________ PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From bcdukes <@t> lexhealth.org Mon May 3 08:42:48 2010 From: bcdukes <@t> lexhealth.org (Blake Taylor) Date: Mon May 3 08:42:54 2010 Subject: [Histonet] Barcoding and Tracking Information Message-ID: Does anyone currently use an automated barcoding and tracking system that interfaces with CoPath-Sunquest?? If so can you please tell me which company you are using? Thanks so much Blake Taylor Section Supervisor Dept. of Pathology Lexington Medical Center 803-936-8214 bcdukes@lexhealth.org ______________________________________________________________________ PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From Dorothy.L.Webb <@t> HealthPartners.Com Mon May 3 09:20:23 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon May 3 09:20:31 2010 Subject: [Histonet] Dimethyl sulfoxide Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C579D299F8@HPEMX3.HealthPartners.int> We purchase ours from Sigma-Aldrich. 1-800-558-9160 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From CIngles <@t> uwhealth.org Mon May 3 10:50:30 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Mon May 3 10:52:38 2010 Subject: [Histonet] CD133 Message-ID: Happy Monday! (grumble grumble) I need to know if there are any reference labs out there that do CD133 for stem cells. Our doc wants this done, but we can't find anyone who does it. Thanks Claire From christina.thurby <@t> bms.com Mon May 3 11:07:51 2010 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Mon May 3 11:08:01 2010 Subject: [Histonet] RE: Feulgen/BrdU Dual labeling In-Reply-To: <06bc9134-005a-4290-aad3-5623a2d6eeb3@ushpwbmsmhp001.one.ads.bms.com> References: <06bc9134-005a-4290-aad3-5623a2d6eeb3@ushpwbmsmhp001.one.ads.bms.com> Message-ID: Hi Group, I am re-posting my question from last week, I didn't not have many responses. If anyone has experience with this method, please don't hesitate to e-mail or call me. Is anyone out there doing Feulgen/BrdU Dual labeling? I have the procedure working (Blue Feulgen kit from Scytek) first and then commercially available BrdU antibody using standard ABC detection method. I am using a post antigen retrieval step after the Feulgen staining method is completed using Trilogy in the rice steamer. I do not let the antigen retrieval temp exceed 95 C in the rice steamer as I have been able to reproduce staining artifact when exceeding 95 C by just 2-3 C. I am having problems with reproducibility and labeling consistency within liver sections (1+ to 4+ zones). Please contact me if you have experience with this procedure and can help me troubleshoot! Thanks, Christina Thurby christina.thurby@bms.com Bristol Myers Squibb 812-307-2093 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From amylee779 <@t> yahoo.com Mon May 3 11:30:05 2010 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Mon May 3 11:30:09 2010 Subject: [Histonet] keep deparaffinized and rehydrated slides in dH2O for a few days Message-ID: <923438.72785.qm@web38006.mail.mud.yahoo.com> Hi histonetters, ? Is it safe to keep deparaffinized and rehydrated slides in dH2O at 4 degree for 4 to 5 days before doing IHC? ? Thanks, ? Amy From histonet.nospam <@t> vneubert.com Mon May 3 11:39:44 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Mon May 3 11:39:51 2010 Subject: [Histonet] keep deparaffinized and rehydrated slides in dH2O for a few days In-Reply-To: <923438.72785.qm@web38006.mail.mud.yahoo.com> References: <923438.72785.qm@web38006.mail.mud.yahoo.com> Message-ID: <4BDEFC50.40704@vneubert.com> No, I would not do that. Why don't you dehydrate again an put some drops of paraffin on it? From JEllin <@t> yumaregional.org Mon May 3 11:57:45 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon May 3 11:57:50 2010 Subject: [Histonet] Barcoding and Tracking Information In-Reply-To: Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C62B1@EXCHANGECLUSTER.yumaregional.local> There are several systems that are out there. But I am glad to see that you are wanting to interface with the LIS. That is a key step. But I would look at all of them and come up with a pretty good RFP process for selection. Make sure also they are live with at least 3 to 4 sites with the interface. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blake Taylor Sent: Monday, May 03, 2010 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Barcoding and Tracking Information Does anyone currently use an automated barcoding and tracking system that interfaces with CoPath-Sunquest?? If so can you please tell me which company you are using? Thanks so much Blake Taylor Section Supervisor Dept. of Pathology Lexington Medical Center 803-936-8214 bcdukes@lexhealth.org ______________________________________________________________________ PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From cbarone <@t> NEMOURS.ORG Mon May 3 12:29:28 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon May 3 12:29:34 2010 Subject: [Histonet] Region II Symposium Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7CA6@wlmmsx01.nemours.org> Histonetter's: For anyone planning to attend the Region II Sympoium....I wanted to give you a quick reminder that Monday May 10th, 2010 is the final day to get the discounted room rate at the Clarion Hotel for the Region II.. If you need information on the meeting you can find it through the NJ Society or any state society in the region ( DE, MD, PA, NJ, VA, W.VA amd District of Columbia)...and also NSH. The vendors attending will be many, and this will present a rare opportunity to get thos needed CEUs and network as well. Check out the program...make your selections...but, hurry on those discounted room rates....time is running out for that one! As the Region Director, I look forward to touching base with you all on the questions and suggestions you have for our Region! See you soon! Carol Barone From lpjones <@t> srhs-pa.org Mon May 3 13:15:45 2010 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Mon May 3 13:15:51 2010 Subject: [Histonet] Switching Back to Formalin Message-ID: <4AE8039AEA096143B965CBC6D0921668022CA806BA@EXCH2007.srhs-pa.org> After years of working to get away from the use of Formalin in our lab, it seems as though we are going to be bringing it back. I'm seeking advice concerning what everyone is using to neutralize their formalin prior to disposal. Any recommendations welcome! Thanks in advance! Laura From Bauer.Karen <@t> mayo.edu Mon May 3 13:34:35 2010 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Mon May 3 13:34:42 2010 Subject: [Histonet] Switching Back to Formalin In-Reply-To: <4AE8039AEA096143B965CBC6D0921668022CA806BA@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D0921668022CA806BA@EXCH2007.srhs-pa.org> Message-ID: <53FC421CC200C5429929EDE6C3676F30E355FA@msgebe34> Don't dispose of it... Recycle it. Creative Waste Solutions has a nice "Green" recycler for smaller labs, but we're using the BR Instrument for our lab. I never imagined that we would recycle our formalin, but when we tried out the BR, I was very impressed. We've saved so much by not having to constantly purchase formalin and our surgery department has quit ordering it as well. Since we have much more recycled than we can use, surgery now gets their formalin from us. If you can't get a recycler, we used to use D'Formalizer from Surgipath to neutralize our formalin. Good luck, Karen Karen L. Bauer HT/HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Monday, May 03, 2010 1:16 PM To: Histonet (E-mail) Subject: [Histonet] Switching Back to Formalin After years of working to get away from the use of Formalin in our lab, it seems as though we are going to be bringing it back. I'm seeking advice concerning what everyone is using to neutralize their formalin prior to disposal. Any recommendations welcome! Thanks in advance! Laura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Mon May 3 14:06:40 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Mon May 3 14:06:47 2010 Subject: [Histonet] Switching Back to Formalin In-Reply-To: <53FC421CC200C5429929EDE6C3676F30E355FA@msgebe34> References: <4AE8039AEA096143B965CBC6D0921668022CA806BA@EXCH2007.srhs-pa.org>, <53FC421CC200C5429929EDE6C3676F30E355FA@msgebe34> Message-ID: I suggest Creative Waste Solutions for your Formalin recycling (actually refilering). We are a large volume lab and have been using multiple sytems (3 gallon to 20 gallon) for 5+ yrs without issue. By refiltering, you can reduce your purchase of new solution by at least 70%. There is no end point for re-use and there is no fish ordor as you get from heat distilation. Very cost effective and very user friendly. William DeSalvo, B.S., HTL(ASCP) > Date: Mon, 3 May 2010 13:34:35 -0500 > From: Bauer.Karen@mayo.edu > To: lpjones@srhs-pa.org; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Switching Back to Formalin > CC: > > Don't dispose of it... Recycle it. Creative Waste Solutions has a nice > "Green" recycler for smaller labs, but we're using the BR Instrument for > our lab. I never imagined that we would recycle our formalin, but when > we tried out the BR, I was very impressed. We've saved so much by not > having to constantly purchase formalin and our surgery department has > quit ordering it as well. Since we have much more recycled than we can > use, surgery now gets their formalin from us. > > If you can't get a recycler, we used to use D'Formalizer from Surgipath > to neutralize our formalin. > > Good luck, > > Karen > > Karen L. Bauer HT/HTL (ASCP) > Histology Section Chief > Department of Pathology - Luther Hospital > Luther Midelfort - Mayo Health System > 715-838-3205 > bauer.karen@mayo.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, > Laura > Sent: Monday, May 03, 2010 1:16 PM > To: Histonet (E-mail) > Subject: [Histonet] Switching Back to Formalin > > After years of working to get away from the use of Formalin in our lab, > it seems as though we are going to be bringing it back. I'm seeking > advice concerning what everyone is using to neutralize their formalin > prior to disposal. Any recommendations welcome! Thanks in advance! > Laura > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From jstaruk <@t> masshistology.com Mon May 3 14:30:36 2010 From: jstaruk <@t> masshistology.com (jstaruk) Date: Mon May 3 14:30:36 2010 Subject: [Histonet] ? about a Leica RM2155 microtome In-Reply-To: Message-ID: <214D2296EFB74FBDA1E06471374B2D35@JimPC> Is anyone out there using this machine? I was offered one without the foot switch. I was told this microtome will only work with the foot switch and cannot be operated manually. Can someone confirm this for me? Thanks Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com From napoli <@t> siscom.net Mon May 3 14:31:31 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Mon May 3 14:31:34 2010 Subject: [Histonet] mouse kidney frozen sectioning Message-ID: <4bdf2493.18f.2fc0.192170036@siscom.net> Having trouble with freezing artifact in the form of tiny fissures or cracks in mouse kidney on frozen section. Tissue is paraformaldehyde fixed and infiltrated w 70% aqueous sucrose OCT solution. Anyone else seen this and know how to deal with it? Thx From napoli <@t> siscom.net Mon May 3 14:37:20 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Mon May 3 14:37:24 2010 Subject: [Histonet] C1FE5960057C084CA389CE97779062904EBD096D@TCDMSG01.ad.TexasChildrensHospital.org Message-ID: <4bdf25f0.10a.329b.1625663379@siscom.net> What procedures do you need to know? From ratliffjack <@t> hotmail.com Mon May 3 14:42:06 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon May 3 14:42:18 2010 Subject: [Histonet] ? about a Leica RM2155 microtome In-Reply-To: <214D2296EFB74FBDA1E06471374B2D35@JimPC> References: <214D2296EFB74FBDA1E06471374B2D35@JimPC> Message-ID: This is not true. You have to press the two blue buttons at the same time on the control panel to engage the motor. One button says run stop and the other says run enable. Remember that if you are in continuous mode you have to press the run stop button to stop the motor. Jack On May 3, 2010, at 2:30 PM, "jstaruk" wrote: > Is anyone out there using this machine? I was offered one without > the foot > switch. I was told this microtome will only work with the foot > switch and > cannot be operated manually. Can someone confirm this for me? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From 41dmb41 <@t> gmail.com Mon May 3 15:45:30 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Mon May 3 15:45:53 2010 Subject: [Histonet] Trichrome Help Message-ID: I'm trying to troubleshoot a problem we had today with a Trichrome stain and I was wondering if anyone out there could help. We do Gomori Blue Collagen Trichrome Stain... 10 minutes in Weigert's Hematoxylin and then 15 min in the Gomori Blue Collagen stain followed by a quick change through acetic acid, alcohol and xylene. When it's all done, there is this weird "film" that is present over the entire surface of the slide... almost like the thin gray film that you sometimes see when you leave slides too long in the silver solution for a GMS. Anyways, I'm not sure what's causing it and putting the slide in xylene or alcohol for a long time doesn't get rid of it. It can easily be wiped off, but obviously we can't wipe the section off. If anyone could help me narrow down what step might be causing this, I'd appreciate it! Thanks, Drew From PMonfils <@t> Lifespan.org Mon May 3 17:22:22 2010 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon May 3 17:22:28 2010 Subject: [Histonet] Trichrome Help In-Reply-To: References: Message-ID: <4EBFF65383B74D49995298C4976D1D5E06942F94@LSRIEXCH1.lsmaster.lifespan.org> Hi Drew, I don't know precisely what causes this, but I have seen it happen when using a freshly made batch of the dye solution. Also, a very fine whitish precipitate often collects in the bottom of the bottle in which the dye solution is stored, requiring filtration every day before using the solution - but only when the solution is fresh. Once the solution is a couple of weeks old, both problems disappear. Wish I had a better explanation. Paul From macveigh <@t> usc.edu Mon May 3 17:43:55 2010 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Mon May 3 17:43:50 2010 Subject: [Histonet] mouse kidney frozen sectioning References: <4bdf2493.18f.2fc0.192170036@siscom.net> Message-ID: <4A84D2E9DB3C4245A40E27531ABD2035@DFS66DD1> Hi Andrew, You must freeze fast to avoid the needle like crystals forming from the water during freezing. Best - freeze in OCT in liquid Nitrogen. It gets worse if you keep changing the temp of the block. For example keep at -80 then bring to -20 cut than bring the temp down to remove the block from the chuck and put back at -80. Few of those will make your tissue hard to even recognize. Good luck Michelle HTL USC Keck School of Medicine LA, CA ----- Original Message ----- From: "Andrew Burgeson" To: Sent: Monday, May 03, 2010 12:31 PM Subject: [Histonet] mouse kidney frozen sectioning > Having trouble with freezing artifact in the form of tiny > fissures or cracks in mouse kidney on frozen section. > > Tissue is paraformaldehyde fixed and infiltrated w 70% > aqueous sucrose OCT solution. > > Anyone else seen this and know how to deal with it? > > Thx > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From foreightl <@t> gmail.com Mon May 3 18:02:07 2010 From: foreightl <@t> gmail.com (Pat Laurie) Date: Mon May 3 18:02:11 2010 Subject: [Histonet] ? about a Leica RM2155 microtome In-Reply-To: <214D2296EFB74FBDA1E06471374B2D35@JimPC> References: <214D2296EFB74FBDA1E06471374B2D35@JimPC> Message-ID: The RM 2155 does need a foot pedal to run. We've had ours where the connection wasn't secure, it would stop the microtome when it wasn't enabled. My techs use it as a manual mictotome though. The foot pedal just sits behind the micotome. The next generation RM 2255 and RM 2265 don't need the foot pedal to run. On Mon, May 3, 2010 at 12:30 PM, jstaruk wrote: > Is anyone out there using this machine? I was offered one without the foot > switch. I was told this microtome will only work with the foot switch and > cannot be operated manually. Can someone confirm this for me? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com From Gervaip <@t> aol.com Mon May 3 21:53:49 2010 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Mon May 3 21:54:20 2010 Subject: [Histonet] specimen disposal Message-ID: <42ee4.164f7f94.3910e63d@aol.com> Hi, can you tell me the correct/safe way to dispose of the formalin fixed specimens? The fixed specimens should be considered hazardous waste, but the formalin is a chemical hazard. I think having biohazard waste company that burns these specimens with formalin . According to MSDS we should be able to burn the formalin and safely dispose of it that way. The large volumes of formalin off the processors is neutralized before disposal. But we are trying to avoid the labor intensive, hazardous job of emptying each specimen container. That is hazardous exposure since we have no work area to do this in safely. So, to finally get to the point, why can we not throw all the specimens in formalin into biohazard waste bags and containers? Pearl "Dance like no one is watching" From MariAnn.Mailhiot <@t> leica-microsystems.com Tue May 4 08:09:45 2010 From: MariAnn.Mailhiot <@t> leica-microsystems.com (MariAnn.Mailhiot@leica-microsystems.com) Date: Tue May 4 08:09:56 2010 Subject: [Histonet] ? about a Leica RM2155 microtome In-Reply-To: Message-ID: Hi All I thought I would respond to the email. If you don't have a foot switch you can place a dummy plug in the back where the foot pedal goes. You would then have to get a hand pad to run the instrument. If you have questions you are welcome to give me a call. Kind Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com Pat Laurie To Sent by: jstaruk histonet-bounces@ cc lists.utsouthwest histonet ern.edu Subject Re: [Histonet] ? about a Leica 05/03/2010 06:02 RM2155 microtome PM The RM 2155 does need a foot pedal to run. We've had ours where the connection wasn't secure, it would stop the microtome when it wasn't enabled. My techs use it as a manual mictotome though. The foot pedal just sits behind the micotome. The next generation RM 2255 and RM 2265 don't need the foot pedal to run. On Mon, May 3, 2010 at 12:30 PM, jstaruk wrote: > Is anyone out there using this machine? I was offered one without the foot > switch. I was told this microtome will only work with the foot switch and > cannot be operated manually. Can someone confirm this for me? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From mbbarra <@t> uol.com.br Tue May 4 08:29:59 2010 From: mbbarra <@t> uol.com.br (mbbarra) Date: Tue May 4 08:30:17 2010 Subject: [Histonet] Formalin and Kylen recycling Message-ID: <4be02157b6ad9_385fdbc1670197@weasel10.tmail> Some help Has anyone else use use BP instruments for recycling. I need some information about the subject. Marinez Barra Laboratory of Pathology From pathology.histology <@t> gmail.com Tue May 4 09:56:59 2010 From: pathology.histology <@t> gmail.com (path lab) Date: Tue May 4 09:57:10 2010 Subject: [Histonet] waterbath residue Message-ID: Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. From srishan <@t> mail.holyname.org Tue May 4 10:05:25 2010 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Tue May 4 10:06:14 2010 Subject: [Histonet] CBG BIOTECH RECYCLER Message-ID: Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin. Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles. Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated. Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From DNolan <@t> evanhospital.com Tue May 4 10:12:46 2010 From: DNolan <@t> evanhospital.com (Donna M. Nolan) Date: Tue May 4 10:13:02 2010 Subject: [Histonet] grossing qualifications Message-ID: I was asked to get opinions about grossing personnel. We have a tech with an Associate of Science in Medical Assistant with 8 credits in Anatomy and Physiology, 4 credits in Microbiology, 3 credits in Medical Terminology, and 16 credits in Medical Assistant Theory and Practicum. Would you consider her qualified to gross specimens? Thanks, Donna This message has been scanned for malware by Websense. www.websense.com From Janice.Mahoney <@t> alegent.org Tue May 4 10:43:30 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue May 4 10:43:40 2010 Subject: [Histonet] waterbath residue In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A146@EXCHMBC2.ad.ah.local> Are you using pencils to mark your slides? Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of path lab Sent: Tuesday, May 04, 2010 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] waterbath residue Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From liz <@t> premierlab.com Tue May 4 10:43:58 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue May 4 10:44:03 2010 Subject: [Histonet] CBG BIOTECH RECYCLER In-Reply-To: Message-ID: Nirmala We have the CBG recycler also. We initially started recycling both alcohol and xylene, but we now only recycle alcohol. We ended up with so much recycled xylene that we could not use. Also you can not use the recycled xylene on the last xylene station in the tissue processor, or for cleaning. It just did not work out for us for the xylene. We rotate our xylenes and alcohols weekly so we are always putting on one fresh absolute and one fresh xylene, there was never a place for us to use the recycled xylene, so we ended up with lots of it. If you change your entire tissue processor then you could put a recycled xylene in place of your first xylene and then use fresh xylene for the last station. We do recycle the alcohol. We get about 95% alcohol out of the recycler - we test it for percentage of alcohol via a hydrometer and for contamination with xylene by adding water to a small portion of it. We only use it for 95% or less so we make up our 50%, 70% and 80% alcohol from the recycled alcohol, these solutions do go on our tissue processor and in the staining set up and they seem to work just fine. To be honest we do not keep track of how many times the alcohol has been recycled we just keep recycling it. As for disposal we have a really cool flammable cabinet that houses a 55 gallon drum, the drum is on rollers so it can be moved easily. All of our waste goes in there its picked up whenever it is full. We use Source/AET Environmental to dispose of all our liquid waste. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Tuesday, May 04, 2010 9:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CBG BIOTECH RECYCLER Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin. Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles. Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated. Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Tue May 4 10:56:58 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue May 4 10:57:14 2010 Subject: [Histonet] waterbath residue In-Reply-To: References: Message-ID: <4BE00B8A.59CD.00EE.0@hurleymc.com> Those black flecks could absolutely be from the waterbaths. I have yet to find a coated waterbath that does not eventually loose its lining. In frustration, I've had techs use a SOS pad to scrub off the lining to finally get rid of it. Waterbath still worked, just no nice teflon lining. I've tried returning them, but they all still went bad. Maybe next purchase, you could go the glass liner type. >>> path lab 5/4/2010 10:56 AM >>> Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue May 4 11:39:54 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 4 11:39:59 2010 Subject: [Histonet] waterbath residue In-Reply-To: <4BE00B8A.59CD.00EE.0@hurleymc.com> Message-ID: <456410.74283.qm@web65712.mail.ac4.yahoo.com> I used to glue a black X-ray film separating paraffinized sheet to the bottom of the water bath for a black background and changed it as needed. Ren? J. --- On Tue, 5/4/10, Lynette Pavelich wrote: From: Lynette Pavelich Subject: Re: [Histonet] waterbath residue To: "path lab" , histonet@lists.utsouthwestern.edu Date: Tuesday, May 4, 2010, 11:56 AM Those black flecks could absolutely be from the waterbaths.? I have yet to find a coated waterbath that does not eventually loose its lining. In frustration, I've had techs use a SOS pad to scrub off the lining to finally get rid of it.? Waterbath still worked, just no nice teflon lining.? I've tried returning them, but they all still went bad.? Maybe next purchase, you could go the glass liner type. >>> path lab 5/4/2010 10:56 AM >>> Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day.? Is anyone else having this problem?? It appears that the finish is gradually wearing off. Could this be the cause of the???artifact ( black specs appear to be on top of the tissue ) that we occassionally see?? Thank you in advance for your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> auburn.edu Tue May 4 12:01:13 2010 From: gentras <@t> auburn.edu (Atoska Gentry) Date: Tue May 4 12:01:25 2010 Subject: [Histonet] RE: Lynn Burton, Galesburg, IL Message-ID: <4BE00C8A.C676.0026.0@auburn.edu> Hello Lynn Burton of Animal Disease Lab, Galesburg, IL will you please contact me ASAP regarding a 2006 Histonet posting. From ayanava_chak <@t> yahoo.com Tue May 4 12:47:02 2010 From: ayanava_chak <@t> yahoo.com (ayanava chakravarti) Date: Tue May 4 12:47:08 2010 Subject: [Histonet] Histochoice fixation Message-ID: <796214.96321.qm@web53705.mail.re2.yahoo.com> I wish to?test how the Histochoice ( Amresco) ?fixative works in lieu of 4% paraformaldehyde for my frozen brain sections in order to avoid the subsequent antigen retrieval step. Can anybody share their experience how it works ? For how much time should I use it for fixation? Thanks Ayanabha Chakraborti, Ph.D Dept of Neurosurgery University of California, San Francisco. From sbreeden <@t> nmda.nmsu.edu Tue May 4 15:34:28 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue May 4 15:34:35 2010 Subject: [Histonet] Histo Schools? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47098@nmdamailsvr.nmda.ad.nmsu.edu> Is there a path to becoming a histologist in the State of Oklahoma? I know there are online training programs which can be completed concurrently with an Associates degree (I think) but is/are there a location/locations in OK where the clinical portion of the training can be carried out? I'd appreciate any leads - it's for a family member I'm trying to recruit to become One of Us! Thank you, everyone. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From histotech <@t> imagesbyhopper.com Tue May 4 15:39:49 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue May 4 15:39:53 2010 Subject: [Histonet] CBG BIOTECH RECYCLER In-Reply-To: Message-ID: <9AEEB598DB4348569868A92FBB227379@hopperPC> Liz, I am curious, why do you not use the recycled xylene prior to the paraffin step in the processor? And why not for cleaning? I understand not recycling the cleaning alcohol or xylene, or even the first xylene on the stainer as they have too many contaminants. Nirmala, With regards to getting a lower quality of your alcohol, do you check the water content prior to recycling? We have found that it's not worth attempting to recycle any graded alcohols below 95% (that is to say, 70% or 80%). There is a "dilute" alcohol setting that can be used, but you will need the CBG folks to tell you how to access that one. It's there though! Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, May 04, 2010 11:44 AM To: srishan@mail.holyname.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CBG BIOTECH RECYCLER Nirmala We have the CBG recycler also. We initially started recycling both alcohol and xylene, but we now only recycle alcohol. We ended up with so much recycled xylene that we could not use. Also you can not use the recycled xylene on the last xylene station in the tissue processor, or for cleaning. It just did not work out for us for the xylene. We rotate our xylenes and alcohols weekly so we are always putting on one fresh absolute and one fresh xylene, there was never a place for us to use the recycled xylene, so we ended up with lots of it. If you change your entire tissue processor then you could put a recycled xylene in place of your first xylene and then use fresh xylene for the last station. We do recycle the alcohol. We get about 95% alcohol out of the recycler - we test it for percentage of alcohol via a hydrometer and for contamination with xylene by adding water to a small portion of it. We only use it for 95% or less so we make up our 50%, 70% and 80% alcohol from the recycled alcohol, these solutions do go on our tissue processor and in the staining set up and they seem to work just fine. To be honest we do not keep track of how many times the alcohol has been recycled we just keep recycling it. As for disposal we have a really cool flammable cabinet that houses a 55 gallon drum, the drum is on rollers so it can be moved easily. All of our waste goes in there its picked up whenever it is full. We use Source/AET Environmental to dispose of all our liquid waste. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Tuesday, May 04, 2010 9:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CBG BIOTECH RECYCLER Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin. Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles. Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated. Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2852 - Release Date: 05/03/10 18:27:00 From BurdetteP <@t> MedImmune.com Tue May 4 16:08:11 2010 From: BurdetteP <@t> MedImmune.com (Burdette, Paul) Date: Tue May 4 16:08:18 2010 Subject: [Histonet] For Sale Used Leica EG1160 Message-ID: I have a Leica EG1160, 5.5 yrs old, dispenser is the only thing that does not work. -Paul ------------------------------------------------------------------- To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From sraibley <@t> yahoo.com Tue May 4 21:30:01 2010 From: sraibley <@t> yahoo.com (Susan Raibley) Date: Tue May 4 21:30:08 2010 Subject: [Histonet] Re: CBG BIOTECH RECYCLER Message-ID: <417612.74446.qm@web56006.mail.re3.yahoo.com> We use the CBG Biotech Recycler to recycle formalin, alcohol and xylene and they all turn out very nicely. We always assay/test each batch to make sure it is ok to use. The xylene always comes out at 99.9% and we use it on all stations of our processor and to stain with and have no problems. We collect our waste alcohols as batches of 70% and 80% to be recycled together and then 95% and 100% to be recycled together. Ususally we get back between 83% and 98% from the reclaimed alcohol and dilute accordingly. We were told by CBG Biotech Tech Support, there is no limit to how many times you can recycle any of the products. Also, you can recycle xylene with paraffin in it, but DO NOT recycle any alcohol contaminated with xylene. We do not recycle any alcohol off the stainer or the cleaning alcohol off the processors. The waste we end up with that cannot be recycled goes in the 55 gal. barrels to be hauled off for proper disposal. Hope this helps! Susan Bincsik, HT (ASCP) From ian.montgomery <@t> bio.gla.ac.uk Wed May 5 05:46:55 2010 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed May 5 05:50:31 2010 Subject: FW: [Histonet] waterbath residue Message-ID: In the past I've given the water bath a really good clean then sprayed with matt black paint. Couple of coats and it looks like new. The paint that comes in cans from DIY stores is all that's needed. Any paint remaining can be used on the lab wall, "mental histologists rule, ok." It's a Glasgow thing. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 04 May 2010 17:40 To: path lab; histonet@lists.utsouthwestern.edu; Lynette Pavelich Subject: Re: [Histonet] waterbath residue I used to glue a black X-ray film separating paraffinized sheet to the bottom of the water bath for a black background and changed it as needed. Ren? J. --- On Tue, 5/4/10, Lynette Pavelich wrote: From: Lynette Pavelich Subject: Re: [Histonet] waterbath residue To: "path lab" , histonet@lists.utsouthwestern.edu Date: Tuesday, May 4, 2010, 11:56 AM Those black flecks could absolutely be from the waterbaths.? I have yet to find a coated waterbath that does not eventually loose its lining. In frustration, I've had techs use a SOS pad to scrub off the lining to finally get rid of it.? Waterbath still worked, just no nice teflon lining.? I've tried returning them, but they all still went bad.? Maybe next purchase, you could go the glass liner type. >>> path lab 5/4/2010 10:56 AM >>> Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day.? Is anyone else having this problem?? It appears that the finish is gradually wearing off. Could this be the cause of the???artifact ( black specs appear to be on top of the tissue ) that we occassionally see?? Thank you in advance for your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed May 5 07:46:24 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed May 5 07:46:29 2010 Subject: [Histonet] Theresa Schuldt? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4709C@nmdamailsvr.nmda.ad.nmsu.edu> If Theresa Schuldt is "out there", please contact me. Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From tkngflght <@t> yahoo.com Wed May 5 08:31:52 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed May 5 08:31:57 2010 Subject: [Histonet] Costs for derm technical Message-ID: <545635.59019.qm@web50902.mail.re2.yahoo.com> ?How do you split up your technical charges?? ? We're looking at a new client who only wants derm gross and one or two H&E slides per specimen.? I haven't collected enough information to work out our cost per slide and suspect it would be pretty similar to what other labs produce. Can anyone help me split pricing for gross, processing, one H&E, recuts and such? ? Much appreciated--I have to have numbers by later tonight! ? Cheryl tkngflght@yahoo.com 281.883.7704 From Charles.Scouten <@t> leica-microsystems.com Wed May 5 08:34:29 2010 From: Charles.Scouten <@t> leica-microsystems.com (Charles.Scouten@leica-microsystems.com) Date: Wed May 5 08:34:50 2010 Subject: [Histonet] mouse kidney frozen sectioning Message-ID: Cracks and fissues is not freezing artifact, as from freezing too slow. You are cutting the tissue too cold, and/or the blade angle is too steep. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Burgeson Sent: Monday, May 03, 2010 2:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse kidney frozen sectioning Having trouble with freezing artifact in the form of tiny fissures or cracks in mouse kidney on frozen section. Tissue is paraformaldehyde fixed and infiltrated w 70% aqueous sucrose OCT solution. Anyone else seen this and know how to deal with it? Thx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From LSebree <@t> uwhealth.org Wed May 5 08:57:24 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed May 5 08:57:33 2010 Subject: [Histonet] mouse kidney frozen sectioning In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E2738399E39@UWHC-MAIL01.uwhis.hosp.wisc.edu> Cracks and fissures can also result from freezing too fast. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Scouten@leica-microsystems.com Sent: Wednesday, May 05, 2010 8:34 AM To: napoli@siscom.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] mouse kidney frozen sectioning Cracks and fissues is not freezing artifact, as from freezing too slow. You are cutting the tissue too cold, and/or the blade angle is too steep. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Burgeson Sent: Monday, May 03, 2010 2:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse kidney frozen sectioning Having trouble with freezing artifact in the form of tiny fissures or cracks in mouse kidney on frozen section. Tissue is paraformaldehyde fixed and infiltrated w 70% aqueous sucrose OCT solution. Anyone else seen this and know how to deal with it? Thx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 5 09:20:57 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 5 09:21:01 2010 Subject: [Histonet] Costs for derm technical In-Reply-To: <545635.59019.qm@web50902.mail.re2.yahoo.com> Message-ID: <875159.30352.qm@web65701.mail.ac4.yahoo.com> I am sending you something on separate cover. Ren? J. --- On Wed, 5/5/10, Cheryl wrote: From: Cheryl Subject: [Histonet] Costs for derm technical To: histonet@lists.utsouthwestern.edu Date: Wednesday, May 5, 2010, 9:31 AM ?How do you split up your technical charges?? ? We're looking at a new client who only wants derm gross and one or two H&E slides per specimen.? I haven't collected enough information to work out our cost per slide and suspect it would be pretty similar to what other labs produce. Can anyone help me split pricing for gross, processing, one H&E, recuts and such? ? Much appreciated--I have to have numbers by later tonight! ? Cheryl tkngflght@yahoo.com 281.883.7704 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Wed May 5 09:28:10 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed May 5 09:28:41 2010 Subject: [Histonet] Histotech Needed In NY Day Shift Message-ID: Our client, located in Oneonta, NY, has retained Allied Search Partners and MPath Search Partners to assist in the search for a highly qualified Histotechnologist/Histotechnician. This is a unique opportunity to join a financially strong and growing organization in a key role within the company. Shift: Full Time, Monday-Friday, Day Shift, M-T 6am-2:30pm Requirements/Job Description: - licensed clinical laboratory technology practitioner - performs slide based histological assays, tests, and procedures and any other such tests - maintaining equipment and records and performing quality assurance activities relating to procedure performance on histological testing of human tissues and which require limited exercise of independent judgment and is performed under the supervision of a laboratory supervisor - designated by the director of a clinical laboratory or under the supervision of the director of the clinical laboratory. He/she also provides prompt and accurate laboratory test results to providers and internal departments. - Prepares samples for examination and performance of testing following established procedures. - Correlates knowledge and understanding of theory and technical elements of testing to solve problems and answers questions effectively and efficiently. Minimum Requirements: - Prerequisites include an Associate?s degree - Must be qualified as a Histotechnician in accordance to New York State Department of Health regulations - Experience with various histological specimens and techniques are desired - Licensure as a Clinical Laboratory Technician by NYS SED required - Requires the ability to provide high quality laboratory service in both the inpatient and outpatient setting To apply please submit resume to Alyssa@alliedsearchpartners.com for initial prescreening of resume. Please be aware that all resumes are held confidential and no resume will be submitted to client without speaking to you over the phone during a phone screen. To send a referral for this position for a cash bonus please visit WWW.ALLIEDSEARCHPARTNERS.COM From Brenda <@t> nsh.org Wed May 5 10:36:29 2010 From: Brenda <@t> nsh.org (Brenda Royce) Date: Wed May 5 10:36:38 2010 Subject: [Histonet] NSH 500 Race for great education! Message-ID: Happy Wednesday Histo Professionals! In case you haven't heard yet, the NSH 3rd Annual Summer Symposium is coming soon! This year it will be held in the Indianapolis, IN on June 14-15, 2010. Registration is open, so please visit our website www.nsh.org today to reserve your spot or call the NSH office (443)535-4060 for more information. Indy seems to have a lot of winning combinations, such as: * Peyton Manning and the Colts making it to the Superbowl * A small university like Butler making it to the Final 4 in March Madness * Not to forget, someone always wins in the Indy 500! * And now... The NSH Summer Symposium featuring great speakers and education!!! What better place to offer winning education for our histology professionals then in Indy at the NSH 500 (aka NSH3rd Annual Summer Symposium). The Summer Symposium offers a variety of topics for all levels. Great opportunity for students as well as established histotechs to learn new things while earning your needed contact hours. Don't be the last one to cross the finish line, start your engines and REGISTER TODAY! Come and Join the Fun! I hope to see you there. From Farnana <@t> nehealth.com Wed May 5 12:06:06 2010 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Wed May 5 12:06:23 2010 Subject: [Histonet] Clo Test CPT Message-ID: <4BE16D3E.26ED.00D9.1@nehealth.com> Hi everyone, I am looking to see what CPT code everyone is using for reading Clo Tests in the pathology department. I have heard of using 87081 but I am not sure if this is accurate as this is for culture and the CLO is biochemical reaction not a culture. Currently I have been using 88300 gross only. Any help would be appreciated. Thank you, Amy Farnan Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From mdeguzman <@t> lifecell.com Wed May 5 12:09:58 2010 From: mdeguzman <@t> lifecell.com (DeGuzman, Maria) Date: Wed May 5 12:10:03 2010 Subject: [Histonet] test Message-ID: <5476245379016B4D8212E8BCD11ECFFBB77A4D56@AMWPVEX01.kci.com> test Maria V. De Guzman| Histology Technician I Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1482 Email mdeguzman@lifecell.com Mobile 732.688.1386 www.Lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 From mdeguzman <@t> lifecell.com Wed May 5 12:11:21 2010 From: mdeguzman <@t> lifecell.com (DeGuzman, Maria) Date: Wed May 5 12:11:29 2010 Subject: [Histonet] test In-Reply-To: <5d1ac7fb-2d28-4826-8fd1-e4dc488bebbd@amwpht01.kci.com> References: <5d1ac7fb-2d28-4826-8fd1-e4dc488bebbd@amwpht01.kci.com> Message-ID: <5476245379016B4D8212E8BCD11ECFFBB77A4D5C@AMWPVEX01.kci.com> test Maria V. De Guzman| Histology Technician I Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1482 Email mdeguzman@lifecell.com Mobile 732.688.1386 www.Lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, May 04, 2010 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Region II Symposium (Barone, Carol ) 2. Switching Back to Formalin (Jones, Laura) 3. RE: Switching Back to Formalin (Bauer, Karen L.) 4. RE: Switching Back to Formalin (WILLIAM DESALVO) 5. ? about a Leica RM2155 microtome (jstaruk) 6. mouse kidney frozen sectioning (Andrew Burgeson) 7. C1FE5960057C084CA389CE97779062904EBD096D@TCDMSG01.ad.TexasChildrensHospital.org (Andrew Burgeson) 8. Re: ? about a Leica RM2155 microtome (Jack Ratliff) 9. Trichrome Help (Drew Meyer) 10. RE: Trichrome Help (Monfils, Paul) 11. Re: mouse kidney frozen sectioning (Michelle MacVeigh-Aloni) 12. Re: ? about a Leica RM2155 microtome (Pat Laurie) 13. specimen disposal (Gervaip@aol.com) 14. Re: ? about a Leica RM2155 microtome (MariAnn.Mailhiot@leica-microsystems.com) 15. Formalin and Kylen recycling (mbbarra) 16. waterbath residue (path lab) 17. CBG BIOTECH RECYCLER (srishan@mail.holyname.org) 18. grossing qualifications (Donna M. Nolan) 19. RE: waterbath residue (Mahoney,Janice A) 20. RE: CBG BIOTECH RECYCLER (Liz Chlipala) 21. Re: waterbath residue (Lynette Pavelich) 22. Re: waterbath residue (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 3 May 2010 13:29:28 -0400 From: "Barone, Carol " Subject: [Histonet] Region II Symposium To: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7CA6@wlmmsx01.nemours.org> Content-Type: text/plain; charset="us-ascii" Histonetter's: For anyone planning to attend the Region II Sympoium....I wanted to give you a quick reminder that Monday May 10th, 2010 is the final day to get the discounted room rate at the Clarion Hotel for the Region II.. If you need information on the meeting you can find it through the NJ Society or any state society in the region ( DE, MD, PA, NJ, VA, W.VA amd District of Columbia)...and also NSH. The vendors attending will be many, and this will present a rare opportunity to get thos needed CEUs and network as well. Check out the program...make your selections...but, hurry on those discounted room rates....time is running out for that one! As the Region Director, I look forward to touching base with you all on the questions and suggestions you have for our Region! See you soon! Carol Barone ------------------------------ Message: 2 Date: Mon, 3 May 2010 14:15:45 -0400 From: "Jones, Laura" Subject: [Histonet] Switching Back to Formalin To: "Histonet (E-mail)" Message-ID: <4AE8039AEA096143B965CBC6D0921668022CA806BA@EXCH2007.srhs-pa.org> Content-Type: text/plain; charset="iso-8859-1" After years of working to get away from the use of Formalin in our lab, it seems as though we are going to be bringing it back. I'm seeking advice concerning what everyone is using to neutralize their formalin prior to disposal. Any recommendations welcome! Thanks in advance! Laura ------------------------------ Message: 3 Date: Mon, 3 May 2010 13:34:35 -0500 From: "Bauer, Karen L." Subject: RE: [Histonet] Switching Back to Formalin To: "Jones, Laura" , "Histonet (E-mail)" Message-ID: <53FC421CC200C5429929EDE6C3676F30E355FA@msgebe34> Content-Type: text/plain; charset="us-ascii" Don't dispose of it... Recycle it. Creative Waste Solutions has a nice "Green" recycler for smaller labs, but we're using the BR Instrument for our lab. I never imagined that we would recycle our formalin, but when we tried out the BR, I was very impressed. We've saved so much by not having to constantly purchase formalin and our surgery department has quit ordering it as well. Since we have much more recycled than we can use, surgery now gets their formalin from us. If you can't get a recycler, we used to use D'Formalizer from Surgipath to neutralize our formalin. Good luck, Karen Karen L. Bauer HT/HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Monday, May 03, 2010 1:16 PM To: Histonet (E-mail) Subject: [Histonet] Switching Back to Formalin After years of working to get away from the use of Formalin in our lab, it seems as though we are going to be bringing it back. I'm seeking advice concerning what everyone is using to neutralize their formalin prior to disposal. Any recommendations welcome! Thanks in advance! Laura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 3 May 2010 13:06:40 -0600 From: WILLIAM DESALVO Subject: RE: [Histonet] Switching Back to Formalin To: , , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I suggest Creative Waste Solutions for your Formalin recycling (actually refilering). We are a large volume lab and have been using multiple sytems (3 gallon to 20 gallon) for 5+ yrs without issue. By refiltering, you can reduce your purchase of new solution by at least 70%. There is no end point for re-use and there is no fish ordor as you get from heat distilation. Very cost effective and very user friendly. William DeSalvo, B.S., HTL(ASCP) > Date: Mon, 3 May 2010 13:34:35 -0500 > From: Bauer.Karen@mayo.edu > To: lpjones@srhs-pa.org; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Switching Back to Formalin > CC: > > Don't dispose of it... Recycle it. Creative Waste Solutions has a nice > "Green" recycler for smaller labs, but we're using the BR Instrument for > our lab. I never imagined that we would recycle our formalin, but when > we tried out the BR, I was very impressed. We've saved so much by not > having to constantly purchase formalin and our surgery department has > quit ordering it as well. Since we have much more recycled than we can > use, surgery now gets their formalin from us. > > If you can't get a recycler, we used to use D'Formalizer from Surgipath > to neutralize our formalin. > > Good luck, > > Karen > > Karen L. Bauer HT/HTL (ASCP) > Histology Section Chief > Department of Pathology - Luther Hospital > Luther Midelfort - Mayo Health System > 715-838-3205 > bauer.karen@mayo.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, > Laura > Sent: Monday, May 03, 2010 1:16 PM > To: Histonet (E-mail) > Subject: [Histonet] Switching Back to Formalin > > After years of working to get away from the use of Formalin in our lab, > it seems as though we are going to be bringing it back. I'm seeking > advice concerning what everyone is using to neutralize their formalin > prior to disposal. Any recommendations welcome! Thanks in advance! > Laura > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 ------------------------------ Message: 5 Date: Mon, 3 May 2010 15:30:36 -0400 From: "jstaruk" Subject: [Histonet] ? about a Leica RM2155 microtome To: "'histonet'" Message-ID: <214D2296EFB74FBDA1E06471374B2D35@JimPC> Content-Type: text/plain; charset="us-ascii" Is anyone out there using this machine? I was offered one without the foot switch. I was told this microtome will only work with the foot switch and cannot be operated manually. Can someone confirm this for me? Thanks Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com ------------------------------ Message: 6 Date: Mon, 03 May 2010 15:31:31 -0400 From: "Andrew Burgeson" Subject: [Histonet] mouse kidney frozen sectioning To: histonet@lists.utsouthwestern.edu Message-ID: <4bdf2493.18f.2fc0.192170036@siscom.net> Content-Type: text/plain; charset="iso-8859-1" Having trouble with freezing artifact in the form of tiny fissures or cracks in mouse kidney on frozen section. Tissue is paraformaldehyde fixed and infiltrated w 70% aqueous sucrose OCT solution. Anyone else seen this and know how to deal with it? Thx ------------------------------ Message: 7 Date: Mon, 03 May 2010 15:37:20 -0400 From: "Andrew Burgeson" Subject: [Histonet] C1FE5960057C084CA389CE97779062904EBD096D@TCDMSG01.ad.TexasChildrensHospital.org To: histonet@lists.utsouthwestern.edu Message-ID: <4bdf25f0.10a.329b.1625663379@siscom.net> Content-Type: text/plain; charset="iso-8859-1" What procedures do you need to know? ------------------------------ Message: 8 Date: Mon, 3 May 2010 14:42:06 -0500 From: Jack Ratliff Subject: Re: [Histonet] ? about a Leica RM2155 microtome To: jstaruk Cc: histonet Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes This is not true. You have to press the two blue buttons at the same time on the control panel to engage the motor. One button says run stop and the other says run enable. Remember that if you are in continuous mode you have to press the run stop button to stop the motor. Jack On May 3, 2010, at 2:30 PM, "jstaruk" wrote: > Is anyone out there using this machine? I was offered one without > the foot > switch. I was told this microtome will only work with the foot > switch and > cannot be operated manually. Can someone confirm this for me? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Mon, 3 May 2010 16:45:30 -0400 From: Drew Meyer <41dmb41@gmail.com> Subject: [Histonet] Trichrome Help To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I'm trying to troubleshoot a problem we had today with a Trichrome stain and I was wondering if anyone out there could help. We do Gomori Blue Collagen Trichrome Stain... 10 minutes in Weigert's Hematoxylin and then 15 min in the Gomori Blue Collagen stain followed by a quick change through acetic acid, alcohol and xylene. When it's all done, there is this weird "film" that is present over the entire surface of the slide... almost like the thin gray film that you sometimes see when you leave slides too long in the silver solution for a GMS. Anyways, I'm not sure what's causing it and putting the slide in xylene or alcohol for a long time doesn't get rid of it. It can easily be wiped off, but obviously we can't wipe the section off. If anyone could help me narrow down what step might be causing this, I'd appreciate it! Thanks, Drew ------------------------------ Message: 10 Date: Mon, 3 May 2010 18:22:22 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] Trichrome Help To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E06942F94@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" Hi Drew, I don't know precisely what causes this, but I have seen it happen when using a freshly made batch of the dye solution. Also, a very fine whitish precipitate often collects in the bottom of the bottle in which the dye solution is stored, requiring filtration every day before using the solution - but only when the solution is fresh. Once the solution is a couple of weeks old, both problems disappear. Wish I had a better explanation. Paul ------------------------------ Message: 11 Date: Mon, 3 May 2010 15:43:55 -0700 From: "Michelle MacVeigh-Aloni" Subject: Re: [Histonet] mouse kidney frozen sectioning To: Message-ID: <4A84D2E9DB3C4245A40E27531ABD2035@DFS66DD1> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi Andrew, You must freeze fast to avoid the needle like crystals forming from the water during freezing. Best - freeze in OCT in liquid Nitrogen. It gets worse if you keep changing the temp of the block. For example keep at -80 then bring to -20 cut than bring the temp down to remove the block from the chuck and put back at -80. Few of those will make your tissue hard to even recognize. Good luck Michelle HTL USC Keck School of Medicine LA, CA ----- Original Message ----- From: "Andrew Burgeson" To: Sent: Monday, May 03, 2010 12:31 PM Subject: [Histonet] mouse kidney frozen sectioning > Having trouble with freezing artifact in the form of tiny > fissures or cracks in mouse kidney on frozen section. > > Tissue is paraformaldehyde fixed and infiltrated w 70% > aqueous sucrose OCT solution. > > Anyone else seen this and know how to deal with it? > > Thx > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Mon, 3 May 2010 16:02:07 -0700 From: Pat Laurie Subject: Re: [Histonet] ? about a Leica RM2155 microtome To: jstaruk Cc: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 The RM 2155 does need a foot pedal to run. We've had ours where the connection wasn't secure, it would stop the microtome when it wasn't enabled. My techs use it as a manual mictotome though. The foot pedal just sits behind the micotome. The next generation RM 2255 and RM 2265 don't need the foot pedal to run. On Mon, May 3, 2010 at 12:30 PM, jstaruk wrote: > Is anyone out there using this machine? I was offered one without the foot > switch. I was told this microtome will only work with the foot switch and > cannot be operated manually. Can someone confirm this for me? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com ------------------------------ Message: 13 Date: Mon, 3 May 2010 22:53:49 EDT From: Gervaip@aol.com Subject: [Histonet] specimen disposal To: Histonet@lists.utsouthwestern.edu, Jason.Burrill@crl.com, mdoran@siumed.edu Message-ID: <42ee4.164f7f94.3910e63d@aol.com> Content-Type: text/plain; charset="US-ASCII" Hi, can you tell me the correct/safe way to dispose of the formalin fixed specimens? The fixed specimens should be considered hazardous waste, but the formalin is a chemical hazard. I think having biohazard waste company that burns these specimens with formalin . According to MSDS we should be able to burn the formalin and safely dispose of it that way. The large volumes of formalin off the processors is neutralized before disposal. But we are trying to avoid the labor intensive, hazardous job of emptying each specimen container. That is hazardous exposure since we have no work area to do this in safely. So, to finally get to the point, why can we not throw all the specimens in formalin into biohazard waste bags and containers? Pearl "Dance like no one is watching" ------------------------------ Message: 14 Date: Tue, 4 May 2010 08:09:45 -0500 From: MariAnn.Mailhiot@leica-microsystems.com Subject: Re: [Histonet] ? about a Leica RM2155 microtome To: Pat Laurie Cc: histonet , jstaruk , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi All I thought I would respond to the email. If you don't have a foot switch you can place a dummy plug in the back where the foot pedal goes. You would then have to get a hand pad to run the instrument. If you have questions you are welcome to give me a call. Kind Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com Pat Laurie To Sent by: jstaruk histonet-bounces@ cc lists.utsouthwest histonet ern.edu Subject Re: [Histonet] ? about a Leica 05/03/2010 06:02 RM2155 microtome PM The RM 2155 does need a foot pedal to run. We've had ours where the connection wasn't secure, it would stop the microtome when it wasn't enabled. My techs use it as a manual mictotome though. The foot pedal just sits behind the micotome. The next generation RM 2255 and RM 2265 don't need the foot pedal to run. On Mon, May 3, 2010 at 12:30 PM, jstaruk wrote: > Is anyone out there using this machine? I was offered one without the foot > switch. I was told this microtome will only work with the foot switch and > cannot be operated manually. Can someone confirm this for me? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 15 Date: Tue, 4 May 2010 10:29:59 -0300 From: mbbarra Subject: [Histonet] Formalin and Kylen recycling To: histonet@lists.utsouthwestern.edu Message-ID: <4be02157b6ad9_385fdbc1670197@weasel10.tmail> Content-Type: text/plain; charset="utf-8" Some help Has anyone else use use BP instruments for? recycling. I need some information about the subject. Marinez Barra Laboratory of Pathology ------------------------------ Message: 16 Date: Tue, 4 May 2010 10:56:59 -0400 From: path lab Subject: [Histonet] waterbath residue To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. ------------------------------ Message: 17 Date: Tue, 4 May 2010 08:05:25 -0700 From: srishan@mail.holyname.org Subject: [Histonet] CBG BIOTECH RECYCLER To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin. Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles. Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated. Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ------------------------------ Message: 18 Date: Tue, 4 May 2010 11:12:46 -0400 From: "Donna M. Nolan" Subject: [Histonet] grossing qualifications To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I was asked to get opinions about grossing personnel. We have a tech with an Associate of Science in Medical Assistant with 8 credits in Anatomy and Physiology, 4 credits in Microbiology, 3 credits in Medical Terminology, and 16 credits in Medical Assistant Theory and Practicum. Would you consider her qualified to gross specimens? Thanks, Donna This message has been scanned for malware by Websense. www.websense.com ------------------------------ Message: 19 Date: Tue, 4 May 2010 10:43:30 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] waterbath residue To: 'path lab' , "histonet@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A146@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Are you using pencils to mark your slides? Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of path lab Sent: Tuesday, May 04, 2010 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] waterbath residue Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 20 Date: Tue, 4 May 2010 09:43:58 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] CBG BIOTECH RECYCLER To: , Message-ID: Content-Type: text/plain; charset="US-ASCII" Nirmala We have the CBG recycler also. We initially started recycling both alcohol and xylene, but we now only recycle alcohol. We ended up with so much recycled xylene that we could not use. Also you can not use the recycled xylene on the last xylene station in the tissue processor, or for cleaning. It just did not work out for us for the xylene. We rotate our xylenes and alcohols weekly so we are always putting on one fresh absolute and one fresh xylene, there was never a place for us to use the recycled xylene, so we ended up with lots of it. If you change your entire tissue processor then you could put a recycled xylene in place of your first xylene and then use fresh xylene for the last station. We do recycle the alcohol. We get about 95% alcohol out of the recycler - we test it for percentage of alcohol via a hydrometer and for contamination with xylene by adding water to a small portion of it. We only use it for 95% or less so we make up our 50%, 70% and 80% alcohol from the recycled alcohol, these solutions do go on our tissue processor and in the staining set up and they seem to work just fine. To be honest we do not keep track of how many times the alcohol has been recycled we just keep recycling it. As for disposal we have a really cool flammable cabinet that houses a 55 gallon drum, the drum is on rollers so it can be moved easily. All of our waste goes in there its picked up whenever it is full. We use Source/AET Environmental to dispose of all our liquid waste. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Tuesday, May 04, 2010 9:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CBG BIOTECH RECYCLER Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin. Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles. Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated. Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Tue, 04 May 2010 11:56:58 -0400 From: "Lynette Pavelich" Subject: Re: [Histonet] waterbath residue To: "path lab" , Message-ID: <4BE00B8A.59CD.00EE.0@hurleymc.com> Content-Type: text/plain; charset=US-ASCII Those black flecks could absolutely be from the waterbaths. I have yet to find a coated waterbath that does not eventually loose its lining. In frustration, I've had techs use a SOS pad to scrub off the lining to finally get rid of it. Waterbath still worked, just no nice teflon lining. I've tried returning them, but they all still went bad. Maybe next purchase, you could go the glass liner type. >>> path lab 5/4/2010 10:56 AM >>> Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Tue, 4 May 2010 09:39:54 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] waterbath residue To: path lab , histonet@lists.utsouthwestern.edu, Lynette Pavelich Message-ID: <456410.74283.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used to glue a black X-ray film separating paraffinized sheet to the bottom of the water bath for a black background and changed it as needed. Ren? J. --- On Tue, 5/4/10, Lynette Pavelich wrote: From: Lynette Pavelich Subject: Re: [Histonet] waterbath residue To: "path lab" , histonet@lists.utsouthwestern.edu Date: Tuesday, May 4, 2010, 11:56 AM Those black flecks could absolutely be from the waterbaths.? I have yet to find a coated waterbath that does not eventually loose its lining. In frustration, I've had techs use a SOS pad to scrub off the lining to finally get rid of it.? Waterbath still worked, just no nice teflon lining.? I've tried returning them, but they all still went bad.? Maybe next purchase, you could go the glass liner type. >>> path lab 5/4/2010 10:56 AM >>> Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day.? Is anyone else having this problem?? It appears that the finish is gradually wearing off. Could this be the cause of the???artifact ( black specs appear to be on top of the tissue ) that we occassionally see?? Thank you in advance for your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 4 *************************************** From histotech <@t> imagesbyhopper.com Wed May 5 12:12:01 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed May 5 12:12:21 2010 Subject: [Histonet] Re: CBG BIOTECH RECYCLER In-Reply-To: <417612.74446.qm@web56006.mail.re3.yahoo.com> Message-ID: Susan, A few questions/comments: 1) how do you test for the xylene for purity? 2) we used to make different batches of 95/100 and 70/80%, but we gave up on that! It was too much effort for us. We do well with the 95/100 though. We generally will get 98% - 99% on ours. 3) re: alcohol contaminated with xylene, yes, we do the same thing. On our stainer, we will discard the alcohol after deparaffinization, but keep the alcohol prior to the xylene at the end of the staining run. 4) are you hauling off both alcohol and xylene waste? I have found I have very little waste xylene. Thanks! Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Raibley Sent: Tuesday, May 04, 2010 10:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: CBG BIOTECH RECYCLER We use the CBG Biotech Recycler to recycle formalin, alcohol and xylene and they all turn out very nicely. We always assay/test each batch to make sure it is ok to use. The xylene always comes out at 99.9% and we use it on all stations of our processor and to stain with and have no problems. We collect our waste alcohols as batches of 70% and 80% to be recycled together and then 95% and 100% to be recycled together. Ususally we get back between 83% and 98% from the reclaimed alcohol and dilute accordingly. We were told by CBG Biotech Tech Support, there is no limit to how many times you can recycle any of the products. Also, you can recycle xylene with paraffin in it, but DO NOT recycle any alcohol contaminated with xylene. We do not recycle any alcohol off the stainer or the cleaning alcohol off the processors. The waste we end up with that cannot be recycled goes in the 55 gal. barrels to be hauled off for proper disposal. Hope this helps! Susan Bincsik, HT (ASCP) From maggie.allen <@t> nicewareintl.com Wed May 5 12:53:25 2010 From: maggie.allen <@t> nicewareintl.com (Maggie Allen) Date: Wed May 5 12:53:31 2010 Subject: [Histonet] RE: Histonet Digest, Vol 78, Issue 5 In-Reply-To: <20100505175114.8105D8FCF8@spamfilter1.redanvil.net> References: <20100505175114.8105D8FCF8@spamfilter1.redanvil.net> Message-ID: <6BCD4D0894AE0A4A96B4CB8F6779A5700187477D@NICEWARE-MAIN03.NicewareIntl03.local> Hello, I've replied to a few posts for people looking for verification, validation and tracking systems and have not see any of my replies come over. Am I doing something incorrectly? NICEWARE HAS A NEW ADDRESS! Please note that Niceware International has relocated our headquarters.? Our new contact information is: Niceware International, LLC | 200 South Executive Drive | Suite 200 | Brookfield, Wisconsin 53005 | General: (262) 784-2456 | Toll Free: (888) 894-NICE (6423) | Fax: (262) 784-2495 | Technical Support: (262) 784-2466 Click here for more details. Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel? (810) 629-3930 Cell (215) 200-0268 Corporate Numbers : General: (262) 784-2456 Toll Free: (888) 894-NICE (6423) Fax: (262) 784-2495 Technical Support: (262) 784-2466 Email: maggie.allen@nicewareintl.com www.nicewareintl.com http://healthcare.nicewareintl.com FREE Webinars : Friday, May 7th, 2010 10:30-11:30am CDT NiceLabel Print Center Review (Register) Friday, May 11th, 2010 01:00 - 02:00pm CDT Free NiceLabel Pro Design Training Session (Register) Friday, May 21st, 2010 10:30-11:30am CDT A Technical Review of the Enterprise Print Manager (Register) "The information in this e-mail and any attachments is confidential and may be subject to legal professional privilege. It is intended solely for the attention and use of the named addressee(s). If you are not the intended recipient or person responsible for delivering this information to the intended recipient, please notify the sender immediately. Unless you are the intended recipient or his/her representative you are not authorized to, and must not, read, copy, distribute, use or retain this message or any part of it" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, May 05, 2010 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Lynn Burton, Galesburg, IL (Atoska Gentry) 2. Histochoice fixation (ayanava chakravarti) 3. Histo Schools? (Breeden, Sara) 4. RE: CBG BIOTECH RECYCLER (histotech@imagesbyhopper.com) 5. For Sale Used Leica EG1160 (Burdette, Paul) 6. Re: CBG BIOTECH RECYCLER (Susan Raibley) 7. FW: [Histonet] waterbath residue (Ian Montgomery) 8. Theresa Schuldt? (Breeden, Sara) 9. Costs for derm technical (Cheryl) 10. RE: mouse kidney frozen sectioning (Charles.Scouten@leica-microsystems.com) 11. RE: mouse kidney frozen sectioning (Sebree Linda A) 12. Re: Costs for derm technical (Rene J Buesa) 13. Histotech Needed In NY Day Shift (Alyssa Peterson) 14. NSH 500 Race for great education! (Brenda Royce) ---------------------------------------------------------------------- Message: 1 Date: Tue, 04 May 2010 12:01:13 -0500 From: "Atoska Gentry" Subject: [Histonet] RE: Lynn Burton, Galesburg, IL To: Message-ID: <4BE00C8A.C676.0026.0@auburn.edu> Content-Type: text/plain; charset=US-ASCII Hello Lynn Burton of Animal Disease Lab, Galesburg, IL will you please contact me ASAP regarding a 2006 Histonet posting. ------------------------------ Message: 2 Date: Tue, 4 May 2010 10:47:02 -0700 (PDT) From: ayanava chakravarti Subject: [Histonet] Histochoice fixation To: histonet@lists.utsouthwestern.edu Message-ID: <796214.96321.qm@web53705.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I wish to?test how the Histochoice ( Amresco) ?fixative works in lieu of 4% paraformaldehyde for my frozen brain sections in order to avoid the subsequent antigen retrieval step. Can anybody share their experience how it works ? For how much time should I use it for fixation? Thanks Ayanabha Chakraborti, Ph.D Dept of Neurosurgery University of California, San Francisco. ------------------------------ Message: 3 Date: Tue, 4 May 2010 14:34:28 -0600 From: "Breeden, Sara" Subject: [Histonet] Histo Schools? To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47098@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Is there a path to becoming a histologist in the State of Oklahoma? I know there are online training programs which can be completed concurrently with an Associates degree (I think) but is/are there a location/locations in OK where the clinical portion of the training can be carried out? I'd appreciate any leads - it's for a family member I'm trying to recruit to become One of Us! Thank you, everyone. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 4 Date: Tue, 4 May 2010 16:39:49 -0400 From: Subject: RE: [Histonet] CBG BIOTECH RECYCLER To: Message-ID: <9AEEB598DB4348569868A92FBB227379@hopperPC> Content-Type: text/plain; charset="US-ASCII" Liz, I am curious, why do you not use the recycled xylene prior to the paraffin step in the processor? And why not for cleaning? I understand not recycling the cleaning alcohol or xylene, or even the first xylene on the stainer as they have too many contaminants. Nirmala, With regards to getting a lower quality of your alcohol, do you check the water content prior to recycling? We have found that it's not worth attempting to recycle any graded alcohols below 95% (that is to say, 70% or 80%). There is a "dilute" alcohol setting that can be used, but you will need the CBG folks to tell you how to access that one. It's there though! Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, May 04, 2010 11:44 AM To: srishan@mail.holyname.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CBG BIOTECH RECYCLER Nirmala We have the CBG recycler also. We initially started recycling both alcohol and xylene, but we now only recycle alcohol. We ended up with so much recycled xylene that we could not use. Also you can not use the recycled xylene on the last xylene station in the tissue processor, or for cleaning. It just did not work out for us for the xylene. We rotate our xylenes and alcohols weekly so we are always putting on one fresh absolute and one fresh xylene, there was never a place for us to use the recycled xylene, so we ended up with lots of it. If you change your entire tissue processor then you could put a recycled xylene in place of your first xylene and then use fresh xylene for the last station. We do recycle the alcohol. We get about 95% alcohol out of the recycler - we test it for percentage of alcohol via a hydrometer and for contamination with xylene by adding water to a small portion of it. We only use it for 95% or less so we make up our 50%, 70% and 80% alcohol from the recycled alcohol, these solutions do go on our tissue processor and in the staining set up and they seem to work just fine. To be honest we do not keep track of how many times the alcohol has been recycled we just keep recycling it. As for disposal we have a really cool flammable cabinet that houses a 55 gallon drum, the drum is on rollers so it can be moved easily. All of our waste goes in there its picked up whenever it is full. We use Source/AET Environmental to dispose of all our liquid waste. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Tuesday, May 04, 2010 9:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CBG BIOTECH RECYCLER Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin. Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles. Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated. Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2852 - Release Date: 05/03/10 18:27:00 ------------------------------ Message: 5 Date: Tue, 4 May 2010 17:08:11 -0400 From: "Burdette, Paul" Subject: [Histonet] For Sale Used Leica EG1160 To: Message-ID: Content-Type: text/plain; charset="us-ascii" I have a Leica EG1160, 5.5 yrs old, dispenser is the only thing that does not work. -Paul ------------------------------------------------------------------- To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ------------------------------ Message: 6 Date: Tue, 4 May 2010 19:30:01 -0700 (PDT) From: Susan Raibley Subject: [Histonet] Re: CBG BIOTECH RECYCLER To: histonet@lists.utsouthwestern.edu Message-ID: <417612.74446.qm@web56006.mail.re3.yahoo.com> Content-Type: text/plain; charset=us-ascii We use the CBG Biotech Recycler to recycle formalin, alcohol and xylene and they all turn out very nicely. We always assay/test each batch to make sure it is ok to use. The xylene always comes out at 99.9% and we use it on all stations of our processor and to stain with and have no problems. We collect our waste alcohols as batches of 70% and 80% to be recycled together and then 95% and 100% to be recycled together. Ususally we get back between 83% and 98% from the reclaimed alcohol and dilute accordingly. We were told by CBG Biotech Tech Support, there is no limit to how many times you can recycle any of the products. Also, you can recycle xylene with paraffin in it, but DO NOT recycle any alcohol contaminated with xylene. We do not recycle any alcohol off the stainer or the cleaning alcohol off the processors. The waste we end up with that cannot be recycled goes in the 55 gal. barrels to be hauled off for proper disposal. Hope this helps! Susan Bincsik, HT (ASCP) ------------------------------ Message: 7 Date: Wed, 5 May 2010 11:46:55 +0100 From: "Ian Montgomery" Subject: FW: [Histonet] waterbath residue To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" In the past I've given the water bath a really good clean then sprayed with matt black paint. Couple of coats and it looks like new. The paint that comes in cans from DIY stores is all that's needed. Any paint remaining can be used on the lab wall, "mental histologists rule, ok." It's a Glasgow thing. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 04 May 2010 17:40 To: path lab; histonet@lists.utsouthwestern.edu; Lynette Pavelich Subject: Re: [Histonet] waterbath residue I used to glue a black X-ray film separating paraffinized sheet to the bottom of the water bath for a black background and changed it as needed. Ren? J. --- On Tue, 5/4/10, Lynette Pavelich wrote: From: Lynette Pavelich Subject: Re: [Histonet] waterbath residue To: "path lab" , histonet@lists.utsouthwestern.edu Date: Tuesday, May 4, 2010, 11:56 AM Those black flecks could absolutely be from the waterbaths.? I have yet to find a coated waterbath that does not eventually loose its lining. In frustration, I've had techs use a SOS pad to scrub off the lining to finally get rid of it.? Waterbath still worked, just no nice teflon lining.? I've tried returning them, but they all still went bad.? Maybe next purchase, you could go the glass liner type. >>> path lab 5/4/2010 10:56 AM >>> Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day.? Is anyone else having this problem?? It appears that the finish is gradually wearing off. Could this be the cause of the???artifact ( black specs appear to be on top of the tissue ) that we occassionally see?? Thank you in advance for your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 5 May 2010 06:46:24 -0600 From: "Breeden, Sara" Subject: [Histonet] Theresa Schuldt? To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4709C@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" If Theresa Schuldt is "out there", please contact me. Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 9 Date: Wed, 5 May 2010 06:31:52 -0700 (PDT) From: Cheryl Subject: [Histonet] Costs for derm technical To: histonet@lists.utsouthwestern.edu Message-ID: <545635.59019.qm@web50902.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ?How do you split up your technical charges?? ? We're looking at a new client who only wants derm gross and one or two H&E slides per specimen.? I haven't collected enough information to work out our cost per slide and suspect it would be pretty similar to what other labs produce. Can anyone help me split pricing for gross, processing, one H&E, recuts and such? ? Much appreciated--I have to have numbers by later tonight! ? Cheryl tkngflght@yahoo.com 281.883.7704 ------------------------------ Message: 10 Date: Wed, 5 May 2010 08:34:29 -0500 From: Charles.Scouten@leica-microsystems.com Subject: RE: [Histonet] mouse kidney frozen sectioning To: napoli@siscom.net, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Cracks and fissues is not freezing artifact, as from freezing too slow. You are cutting the tissue too cold, and/or the blade angle is too steep. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Burgeson Sent: Monday, May 03, 2010 2:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse kidney frozen sectioning Having trouble with freezing artifact in the form of tiny fissures or cracks in mouse kidney on frozen section. Tissue is paraformaldehyde fixed and infiltrated w 70% aqueous sucrose OCT solution. Anyone else seen this and know how to deal with it? Thx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 11 Date: Wed, 5 May 2010 08:57:24 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] mouse kidney frozen sectioning To: , , Message-ID: <8C023B4AB999614BA4791BAEB26E2738399E39@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="US-ASCII" Cracks and fissures can also result from freezing too fast. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Scouten@leica-microsystems.com Sent: Wednesday, May 05, 2010 8:34 AM To: napoli@siscom.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] mouse kidney frozen sectioning Cracks and fissues is not freezing artifact, as from freezing too slow. You are cutting the tissue too cold, and/or the blade angle is too steep. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Burgeson Sent: Monday, May 03, 2010 2:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse kidney frozen sectioning Having trouble with freezing artifact in the form of tiny fissures or cracks in mouse kidney on frozen section. Tissue is paraformaldehyde fixed and infiltrated w 70% aqueous sucrose OCT solution. Anyone else seen this and know how to deal with it? Thx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 5 May 2010 07:20:57 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Costs for derm technical To: histonet@lists.utsouthwestern.edu, Cheryl Message-ID: <875159.30352.qm@web65701.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I am sending you something on separate cover. Ren? J. --- On Wed, 5/5/10, Cheryl wrote: From: Cheryl Subject: [Histonet] Costs for derm technical To: histonet@lists.utsouthwestern.edu Date: Wednesday, May 5, 2010, 9:31 AM ?How do you split up your technical charges?? ? We're looking at a new client who only wants derm gross and one or two H&E slides per specimen.? I haven't collected enough information to work out our cost per slide and suspect it would be pretty similar to what other labs produce. Can anyone help me split pricing for gross, processing, one H&E, recuts and such? ? Much appreciated--I have to have numbers by later tonight! ? Cheryl tkngflght@yahoo.com 281.883.7704 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 5 May 2010 10:28:10 -0400 From: Alyssa Peterson Subject: [Histonet] Histotech Needed In NY Day Shift To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 Our client, located in Oneonta, NY, has retained Allied Search Partners and MPath Search Partners to assist in the search for a highly qualified Histotechnologist/Histotechnician. This is a unique opportunity to join a financially strong and growing organization in a key role within the company. Shift: Full Time, Monday-Friday, Day Shift, M-T 6am-2:30pm Requirements/Job Description: - licensed clinical laboratory technology practitioner - performs slide based histological assays, tests, and procedures and any other such tests - maintaining equipment and records and performing quality assurance activities relating to procedure performance on histological testing of human tissues and which require limited exercise of independent judgment and is performed under the supervision of a laboratory supervisor - designated by the director of a clinical laboratory or under the supervision of the director of the clinical laboratory. He/she also provides prompt and accurate laboratory test results to providers and internal departments. - Prepares samples for examination and performance of testing following established procedures. - Correlates knowledge and understanding of theory and technical elements of testing to solve problems and answers questions effectively and efficiently. Minimum Requirements: - Prerequisites include an Associate's degree - Must be qualified as a Histotechnician in accordance to New York State Department of Health regulations - Experience with various histological specimens and techniques are desired - Licensure as a Clinical Laboratory Technician by NYS SED required - Requires the ability to provide high quality laboratory service in both the inpatient and outpatient setting To apply please submit resume to Alyssa@alliedsearchpartners.com for initial prescreening of resume. Please be aware that all resumes are held confidential and no resume will be submitted to client without speaking to you over the phone during a phone screen. To send a referral for this position for a cash bonus please visit WWW.ALLIEDSEARCHPARTNERS.COM ------------------------------ Message: 14 Date: Wed, 5 May 2010 11:36:29 -0400 From: "Brenda Royce" Subject: [Histonet] NSH 500 Race for great education! To: Message-ID: Content-Type: text/plain; charset="us-ascii" Happy Wednesday Histo Professionals! In case you haven't heard yet, the NSH 3rd Annual Summer Symposium is coming soon! This year it will be held in the Indianapolis, IN on June 14-15, 2010. Registration is open, so please visit our website www.nsh.org today to reserve your spot or call the NSH office (443)535-4060 for more information. Indy seems to have a lot of winning combinations, such as: * Peyton Manning and the Colts making it to the Superbowl * A small university like Butler making it to the Final 4 in March Madness * Not to forget, someone always wins in the Indy 500! * And now... The NSH Summer Symposium featuring great speakers and education!!! What better place to offer winning education for our histology professionals then in Indy at the NSH 500 (aka NSH3rd Annual Summer Symposium). The Summer Symposium offers a variety of topics for all levels. Great opportunity for students as well as established histotechs to learn new things while earning your needed contact hours. Don't be the last one to cross the finish line, start your engines and REGISTER TODAY! Come and Join the Fun! I hope to see you there. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 5 *************************************** From zerfasp <@t> ors.od.nih.gov Wed May 5 13:43:47 2010 From: zerfasp <@t> ors.od.nih.gov (Zerfas, Patricia (NIH/OD/ORS) [E]) Date: Wed May 5 13:49:44 2010 Subject: [Histonet] HAM 56 Message-ID: Dear Listservers, I attempted to purchase the antibody HAM 56 (1 ml) through Dako and you can only purchase very large quantities. Does anyone know a source for this antibody in smaller quantities or an adequate replacement? I already tried Linscott's. MAC387 is not an adequate replacement for our laboratory. Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 From maggie.allen <@t> nicewareintl.com Wed May 5 14:23:34 2010 From: maggie.allen <@t> nicewareintl.com (Maggie Allen) Date: Wed May 5 14:23:38 2010 Subject: [Histonet] RE: Barcode and Tracking Information In-Reply-To: <6BCD4D0894AE0A4A96B4CB8F6779A57001874743@NICEWARE-MAIN03.NicewareIntl03.local> References: <20100503170030.9B5688FC4A@spamfilter1.redanvil.net> <6BCD4D0894AE0A4A96B4CB8F6779A57001874743@NICEWARE-MAIN03.NicewareIntl03.local> Message-ID: <6BCD4D0894AE0A4A96B4CB8F6779A57001874785@NICEWARE-MAIN03.NicewareIntl03.local> Hello Blake, LabelClinic HTS is a tracking system for Histology artifacts within the Pathology workflow. The product is designed to provide improved quality control and traceability with the lab. It provides flexible and reliable labeling and tracking of histology requisitions, containers, specimens, blocks and slides. Using bar code technology, LabelClinic HTS provides an optimized workflow that reduces errors and significantly enhances patient safety. It can be used stand alone, or integrated into an LIS / AP software system. Benefits: * Flexible lab configuration for custom lab workflow * Reduce errors and increase efficiency * Just-in-time reporting of artifact locations or last know location * Alerting for sub-process time violations and missing artifacts * Deploy to Desktop PC's or mobile hand held's * Configurable workflow * Supports color selection for slide and cassette printers I would be happy to set up an online WebEx demo of the system for anyone who may be interested. Thank you! Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel? (810) 629-3930 Cell (215) 200-0268 From Janice.Mahoney <@t> alegent.org Wed May 5 14:53:00 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed May 5 14:53:11 2010 Subject: [Histonet] RE: Barcode and Tracking Information In-Reply-To: <6BCD4D0894AE0A4A96B4CB8F6779A57001874785@NICEWARE-MAIN03.NicewareIntl03.local> References: <20100503170030.9B5688FC4A@spamfilter1.redanvil.net> <6BCD4D0894AE0A4A96B4CB8F6779A57001874743@NICEWARE-MAIN03.NicewareIntl03.local> <6BCD4D0894AE0A4A96B4CB8F6779A57001874785@NICEWARE-MAIN03.NicewareIntl03.local> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A157@EXCHMBC2.ad.ah.local> Blake, I think I put in my comments about Ventana's Vantage when you first posted the question, but here goes again.. It is wonderful. Vantage is the best thing to come along in my lifetime as a histo tech! Wonderful for patient safety, easy for the techs to use and a manager's dream for all the data it can provide. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maggie Allen Sent: Wednesday, May 05, 2010 2:24 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Hello Blake, LabelClinic HTS is a tracking system for Histology artifacts within the Pathology workflow. The product is designed to provide improved quality control and traceability with the lab. It provides flexible and reliable labeling and tracking of histology requisitions, containers, specimens, blocks and slides. Using bar code technology, LabelClinic HTS provides an optimized workflow that reduces errors and significantly enhances patient safety. It can be used stand alone, or integrated into an LIS / AP software system. Benefits: * Flexible lab configuration for custom lab workflow * Reduce errors and increase efficiency * Just-in-time reporting of artifact locations or last know location * Alerting for sub-process time violations and missing artifacts * Deploy to Desktop PC's or mobile hand held's * Configurable workflow * Supports color selection for slide and cassette printers I would be happy to set up an online WebEx demo of the system for anyone who may be interested. Thank you! Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel (810) 629-3930 Cell (215) 200-0268 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Janice.Mahoney <@t> alegent.org Wed May 5 14:56:17 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed May 5 14:56:30 2010 Subject: [Histonet] (no subject) Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A158@EXCHMBC2.ad.ah.local> I think I put in my comments about Ventana's Vantage when you first posted the question, but here goes again.. It is wonderful. Vantage is the best thing to come along in my lifetime as a histo tech! Wonderful for patient safety, easy for the techs to use and a manager's dream for all the data it can provide. The techs and PA's in my lab love Vantage. Jan Mahoney Omaha, NE ________________________________ Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From aquavet <@t> gmail.com Wed May 5 15:11:35 2010 From: aquavet <@t> gmail.com (Isabelle Cote) Date: Wed May 5 15:11:47 2010 Subject: [Histonet] disinfection of cardboard slide folder Message-ID: Bonjour, We currently use cardboard slide folders to transport our histology slides from the histology laboratory to the pathologist offices. The histology laboratory is a biosafety level 2 lab while the pathologist offices are in a "non-contaminated" office area. Does any other laboratory have this problem? How do you deal with potential contamination issue from the cardboard folders (which are never disinfected)? Have you considered switching to plastic folders for this reason? Can folders be disinfected? Autoclaved? Any advice or comments on this issue will be greatly appreciated Isabelle C?t? Laboratoire d'expertise en pathologie animale du Qu?bec, Qu?bec From Bill.Tench <@t> pph.org Wed May 5 15:57:20 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed May 5 15:57:39 2010 Subject: [Histonet] clo test In-Reply-To: <20100505203548.2B76E12B215@mail1.pph.org> References: <20100505203548.2B76E12B215@mail1.pph.org> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863055@MAIL1.pph.local> The Clo test is a clinical lab test. You need to go to that part of the CPT coding book (sorry I don't have it available). 88300 is an anatomic code (gross only, ie, it requires examination of a piece of tissue or foreign body) and is entirely inappropriate for this test. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, May 05, 2010 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 78, Issue 6 Hi everyone, I am looking to see what CPT code everyone is using for reading Clo Tests in the pathology department. I have heard of using 87081 but I am not sure if this is accurate as this is for culture and the CLO is biochemical reaction not a culture. Currently I have been using 88300 gross only. Any help would be appreciated. Thank you, Amy Farnan *************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From MadaryJ <@t> MedImmune.com Wed May 5 16:22:22 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed May 5 16:22:30 2010 Subject: [Histonet] IDEA FOR A NEW RECYCLER!!!! Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1301A37FFA@MD1EV002.medimmune.com> Some manufacturer should find a way where you can add all of the chemicals together in one container and when the recycler runs it spits out formalin in one, xylene/hemo-de/f83/ in another and alcohol in another all from one run. To opine on another histnet query from earlier I think both have their place. I use CBG for xylene, form, hemo de and alcohol using the hemo on the processor and depar, alcohol on processor for low grade alc, and xyle for all but the last xylen on the stainer using frsh for that. Creative waste is good and simple but the only thing is mixing old and new formalin can't be good long term. What I was thinking about doing was using creative waste gravimetric for the first run, and then after that use the CBG to redistill NBF for round 2, 3, 4 etc. Hey where am I getting all this money and space? Still like BR recycler too, just do not have one anymore, but liked it. All recycling is good as long as people know which ones to use. Still seems to be an issue for some people. I worked in a place where the techs thought you could throw everyting in one container and the rcycler would spit out clean alcohol, xylene and formalin from one collective run. Hey manufactures? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From raestask <@t> grics.net Wed May 5 17:32:47 2010 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Wed May 5 17:33:04 2010 Subject: [Histonet] RE: Barcode and Tracking Information In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A157@EXCHMBC2.ad.ah.local> References: <20100503170030.9B5688FC4A@spamfilter1.redanvil.net><6BCD4D0894AE0A4A96B4CB8F6779A57001874743@NICEWARE-MAIN03.NicewareIntl03.local><6BCD4D0894AE0A4A96B4CB8F6779A57001874785@NICEWARE-MAIN03.NicewareIntl03.local> <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A157@EXCHMBC2.ad.ah.local> Message-ID: <926351C712074D27B3AC517297DF0A72@your4105e587b6> Jan, Ditto! Ditto! Ditto! Couldn't have said it better myself! Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, May 05, 2010 2:53 PM To: 'Maggie Allen'; histonet@lists.utsouthwestern.edu Cc: histonet-request@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Blake, I think I put in my comments about Ventana's Vantage when you first posted the question, but here goes again.. It is wonderful. Vantage is the best thing to come along in my lifetime as a histo tech! Wonderful for patient safety, easy for the techs to use and a manager's dream for all the data it can provide. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maggie Allen Sent: Wednesday, May 05, 2010 2:24 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Hello Blake, LabelClinic HTS is a tracking system for Histology artifacts within the Pathology workflow. The product is designed to provide improved quality control and traceability with the lab. It provides flexible and reliable labeling and tracking of histology requisitions, containers, specimens, blocks and slides. Using bar code technology, LabelClinic HTS provides an optimized workflow that reduces errors and significantly enhances patient safety. It can be used stand alone, or integrated into an LIS / AP software system. Benefits: * Flexible lab configuration for custom lab workflow * Reduce errors and increase efficiency * Just-in-time reporting of artifact locations or last know location * Alerting for sub-process time violations and missing artifacts * Deploy to Desktop PC's or mobile hand held's * Configurable workflow * Supports color selection for slide and cassette printers I would be happy to set up an online WebEx demo of the system for anyone who may be interested. Thank you! Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel (810) 629-3930 Cell (215) 200-0268 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed May 5 22:15:03 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed May 5 22:16:11 2010 Subject: [Histonet] CD 133 Message-ID: Thought I'd try again... Anyone know where I can send some slides for a CD133? Claire From gordon <@t> 10db.co.uk Thu May 6 03:16:36 2010 From: gordon <@t> 10db.co.uk (Gordon Brown) Date: Thu May 6 03:16:45 2010 Subject: [Histonet] Disposable blade holder - amateur microscopist looking for a cheap one! Message-ID: I dabble in microscopy - well I say I dabble, my wife says otherwise! - and I have a reasonably well equipped home lab, which includes the ubiquitous Cambridge Rocker. I've had reasonably good results with plant tissue sections but sharpening the blades has always proved to be a pain and I tend to get mixed results. However, I've recently acquired a bunch of Accu Edge low profile blades at very low cost but I'm unable to source a holder at an affordable price. IS there anyone out there in professional histology land who has for sale a used, even rough condition holder suitable for these blades? You'd get the grateful thanks of both myself and my wife, who will be more than pleased to see me spend less time muttering about my poor sharpening skills! Many thanks Gordon (UK) From gordon <@t> 10db.co.uk Thu May 6 03:36:41 2010 From: gordon <@t> 10db.co.uk (Gordon Brown) Date: Thu May 6 03:36:55 2010 Subject: [Histonet] Disposable blade holder - amateur microscopist looking for a cheap one! Message-ID: Perhaps I should point out that despite the date in the UK, my name is genuinely as given, although my namesake may well be looking for a new hobby to fill in his spare time after today........... Gordon From Goodwd2 <@t> LabCorp.com Thu May 6 07:18:01 2010 From: Goodwd2 <@t> LabCorp.com (Goodwin, Diana) Date: Thu May 6 07:18:10 2010 Subject: [Histonet] NSH Region II Meeting-discount hotel rate deadline is coming up! Message-ID: You don?t want to miss the upcoming Region II Meeting on June 10-12 in Atlantic City NJ! 25 speakers are presenting 30 different topics from wet workshops to short seminars. CEUs will be granted for all sessions. Over 30 vendors are participating in our Exhibit Area. Your registration fee includes free admission to the Vendor Exhibit, AM and PM Breaks, buffet lunch and Friday evening?s reception. The hotel room rate is reduced to $89/night, but you must reserve by Monday, May 10th! The mail-in registration deadline is May 20th. For more information, you can download a meeting brochure from the NSH website under state meetings at www.nsh.org/content/region-ii-meeting. ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA. From eca9 <@t> georgetown.edu Thu May 6 07:56:01 2010 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Thu May 6 07:56:09 2010 Subject: [Histonet] dehydration of hydrated slide.... Message-ID: <4BE2BC61.2040701@georgetown.edu> Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University From LSebree <@t> uwhealth.org Thu May 6 08:49:06 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu May 6 08:49:42 2010 Subject: [Histonet] dehydration of hydrated slide.... In-Reply-To: <4BE2BC61.2040701@georgetown.edu> References: <4BE2BC61.2040701@georgetown.edu> Message-ID: <8C023B4AB999614BA4791BAEB26E2738399E3B@UWHC-MAIL01.uwhis.hosp.wisc.edu> Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MAUGER <@t> email.chop.edu Thu May 6 10:02:59 2010 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Thu May 6 10:04:18 2010 Subject: [Histonet] dehydration of hydrated slide.... In-Reply-To: <8C023B4AB999614BA4791BAEB26E2738399E3B@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <4BE2BC61.2040701@georgetown.edu>, <8C023B4AB999614BA4791BAEB26E2738399E3B@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <443F5B475A9BF647AB962E834884EBAD2788770DE6@EX7CCRPW03V1.chop.edu> Eva, If you dehydrate it again to 100% or xylene, it is less likelt to fall off the slide. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A [LSebree@uwhealth.org] Sent: Thursday, May 06, 2010 9:49 AM To: Eva Permaul; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] dehydration of hydrated slide.... Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Thu May 6 10:20:01 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu May 6 10:20:01 2010 Subject: [Histonet] B5 fixative In-Reply-To: <8C023B4AB999614BA4791BAEB26E2738399E3B@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: I was under the impression that B5, because of the mercury content, was outlawed for use Jan 1, 2005. But now I am not so sure! Can anyone tell me if there is a federal law regarding this? We no longer use B5, we use B+, but I know someone in OK who is using B5 (I am in FL). Is he breaking any laws by using it and/or should he be switched to an alternative like B+? Thanks! Michelle From jqb7 <@t> cdc.gov Thu May 6 10:16:04 2010 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCZVED)) Date: Thu May 6 10:20:44 2010 Subject: [Histonet] myocyte damage Message-ID: <68510B12184E45498EABD4CB6F3868FE012870BC@LTA3VS001.ees.hhs.gov> Hello everyone, I am in need of a special stain or an IHC that will demonstrate myocyte damage in muscle tissue...esp. cardiac. Any help will be greatly appreciated. Thanks! Jeanine Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From tonia.richmond <@t> gracepathology.com Thu May 6 10:27:50 2010 From: tonia.richmond <@t> gracepathology.com (tonia.richmond@gracepathology.com) Date: Thu May 6 10:27:56 2010 Subject: [Histonet] Responses to IHC CAP Validation question In-Reply-To: References: <8CCB51A55105D80-1A38-1C45@webmail-m088.sysops.aol.com>, Message-ID: If you are a brand new lab, how do you validate IHC if you are no yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer Grace Pathology PH: (501) 765-7367 Email: -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- < To: "thisisann@ "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" WPI.EDU Thu May 6 10:42:40 2010 From: shshaw <@t> WPI.EDU (Shaw, Sharon) Date: Thu May 6 10:42:53 2010 Subject: [Histonet] Starting up new lab Message-ID: I'm looking at starting up a new histology lab and need everything, I have a tight budget. Can anybody give me recommendations on where to buy refurbished equipment. Thanks Sharon From Rcartun <@t> harthosp.org Thu May 6 10:57:43 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu May 6 10:57:59 2010 Subject: [Histonet] Formalin fixation time for breast specimens Message-ID: <4BE2AEB7.7400.0077.1@harthosp.org> There has been a lot of discussion recently regarding recommendations for formalin fixation of breast specimens. If you are interested in this topic please read the following article published in the May 2010 issue of the American Journal of Clinical Pathology by Ibarra JA, et al., "Fixation time does not affect the expression of estrogen receptor". Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From BSullivan <@t> shorememorial.org Thu May 6 11:16:45 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Thu May 6 11:18:47 2010 Subject: [Histonet] Responses to IHC CAP Validation question In-Reply-To: Message-ID: One important thing to remember is that you should make sure All material being used for validation is processed the same way. Will your control tissue be purchased or processed in your lab? Control tissue is used for validation but you should use tissue with various levels of positivity. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 tonia.richmond@gr acepathology.com Sent by: To histonet-bounces@ "McMahon, Loralee A" lists.utsouthwest cc "histonet@lists.utsouthwestern.edu" 05/06/2010 11:27 AM , "thisisann@aol.com" Subject RE: [Histonet] Responses to IHC CAP Validation question If you are a brand new lab, how do you validate IHC if you are no= t yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer = / Laboratory Grace Pathology PH: (501) 765-7367 Email: = [1]tonia.= richmond@gracepathology.com -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- <= /FONT> To: "thisisann@= aol.com" , "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" Sent by: histonet-bounces@lists.u= tsouthwestern.edu Date: 04/28/2010 02:01PM Subject: RE: [Histonet] Re= sponses to IHC CAP Validation question Any inspection that I have under= gone we have used the 25 to 30 case rule. Except for the Er/Pr//Her-2= . We use closer to 50 cases. We also use a TMA to make our live= s easier. The TMA contains known positives and known negatives. I= n cases of t-cell or b-cell markers or cytokeratins. 25 to 30 cases i= s easy. But when you are validated for more hard to find markers (SV-= 40) then fewer cases is acceptable. We always throw in a slide that w= e know will not stain for sv-40 like a tonsil - then you can say it has spe= cificity. Any inspector that I have come across is usually understanding= of this. But I am sure that there are exceptions to this.........esp ecially if they are not familiar with immunohistochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong M= emorial Hospital Department of Surgical Pathology (585) 275-7210 ______________________ 5F__= _______________ From: histonet-bounces@lis= ts.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf= Of thisisann@aol.com [thisisann@aol.com] Sent: Wednesday, April 28, 201= 0 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] R= esponses to IHC CAP Validation question The following is one respone= I rec'd: 1. I asked CAP who told me that they do not currentl= y have a guideline on validating but that they recommend what is in t= he following book: Quality Management In Anatomic Pathology, Promoting P= atient Safety Through Systems Improvement and Error by Raouf E. Nakhl= eh, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8-= Quality Management in IHC That is what we follow. I. Get a new antib= ody and optimize it with your positive control. II. Once optimized you n= eed to run it on cases expected to be positive (how many?) "a suffien= t size ..." III. Must also be run on cases expected to be negative. (how= many? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just = that. (ex. some of the hormones we just use a pituitary) ___________________ ______________________ 5F__= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu = [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________ 5F__ ______________________ Histo= net mailing list Histonet@lists.utsouthwestern.edu [3]http://l= ists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:tonia.richmond@gracepathology.com" 2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 3. 3D"http://=/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Thu May 6 11:30:50 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu May 6 11:31:28 2010 Subject: [Histonet] myocyte damage In-Reply-To: <68510B12184E45498EABD4CB6F3868FE012870BC@LTA3VS001.ees.hhs.gov> References: <68510B12184E45498EABD4CB6F3868FE012870BC@LTA3VS001.ees.hhs.gov> Message-ID: <5AFA0A6D83365A1F044D4E16@CDYwxp1931.ad.med.buffalo.edu> Stain with cTnI and inspect for damage visually. Just a guess. Regards, Merced --On Thursday, May 06, 2010 11:16 AM -0400 "Bartlett, Jeanine (CDC/OID/NCZVED)" wrote: > Hello everyone, > > I am in need of a special stain or an IHC that will demonstrate myocyte > damage in muscle tissue...esp. cardiac. > > Any help will be greatly appreciated. > > Thanks! > Jeanine Bartlett > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From sraibley <@t> yahoo.com Thu May 6 12:21:03 2010 From: sraibley <@t> yahoo.com (Susan Raibley) Date: Thu May 6 12:21:09 2010 Subject: [Histonet] Re: CBG BIOTECH RECYCLER Message-ID: <847527.3354.qm@web56006.mail.re3.yahoo.com> Michelle, ? The xylene?purity test?is a very simple. Using a 100 ml graduated cylinder. You just add 85 ml of recycled xylene making sure the bottom of the meniscus aligns right at the top edge of the 85 ml mark and then 15 ml of distilled water, making sure the bottom of the meniscus aligns with the top edge of the 100 ml mark.?Then using your hand as a stopper, invert the graduate and allow mixture to settle and then place back down and record the separation point at the bottom of the meniscus as the separation point...it should be near 15 ml. Then you subtract 15 from the separation point. The remainder plus 0.1 correction factor is the concentration of recoverd xylene. Ex: If your meniscus aligns at 15.5 ml, you take (15.5 - 15) + 0.1 = 0.6 impurities. Therefore, your concentration of recovered xylene is 99.4%. I can e-mail you this page from the operation manual so you can print it. ? All of the information on testing/dillution/assaying formalin, xylene and alcohols should be in the operation manual provided with your recycler. There is a very simple to use alcohol dilution chart in there. You can get a free replacement if you need one by contacting CBG Biotech at 1-800-941-9484. The product # for our manual which has this info is MIS2001. ? We haul away alcohol and some formalin, but usually not xylene, since we keep recycling it. At least it has been a very long time since we had to...only when we just get too backed up to recycle it. We work in research and sometimes our volumes of formalin are so great, we have to haul some away, but only as a last resort, so we mostly haul our alcohol away. ? I hope this helps! ? Susan Bincsik, HT (ASCP) RE: [Histonet] Re: CBG BIOTECH RECYCLER Wednesday, May 5, 2010 1:12 PM From: histotech@imagesbyhopper.com To: histonet@lists.utsouthwestern.edu Susan, A few questions/comments: 1)? how do you test for the xylene for purity? 2)? we used to make different batches of 95/100 and 70/80%, but we gave up on that!? It was too much effort for us.? We do well with the 95/100 though. We generally will get 98% - 99% on ours. 3)? re: alcohol contaminated with xylene, yes, we do the same thing.? On our stainer, we will discard the alcohol after deparaffinization, but keep the alcohol prior to the xylene at the end of the staining run. 4)? are you hauling off both alcohol and xylene waste?? I have found I have very little waste xylene. Thanks! Michelle From pruegg <@t> ihctech.net Thu May 6 12:32:42 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu May 6 12:33:25 2010 Subject: SPAM-LOW: RE: [Histonet] Responses to IHC CAP Validation question In-Reply-To: References: <8CCB51A55105D80-1A38-1C45@webmail-m088.sysops.aol.com>, Message-ID: <100660BA19F543ED9F9FCE867D63FBBB@Patsyoffice> I believe the point of validating is supposed to be to use samples that are processed in your own facility, so if you use controls you have not fixed and processed yourself it would not be valid. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tonia.richmond@gracepathology.com Sent: Thursday, May 06, 2010 9:28 AM To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu; thisisann@aol.com Subject: SPAM-LOW: RE: [Histonet] Responses to IHC CAP Validation question If you are a brand new lab, how do you validate IHC if you are no= yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer = Laboratory Grace Pathology PH: (501) 765-7367 Email: =1]tonia.=ichmond@gracepathology.com -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- <=FONT> To: "thisisann@= aol.com" , "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" Sent by: histonet-bounces@lists.u=southwestern.edu Date: 04/28/2010 02:01PM Subject: RE: [Histonet] Re=ponses to IHC CAP Validation question Any inspection that I have under=one we have used the 25 to 30 case rule. Except for the Er/Pr//Her-2= We use closer to 50 cases. We also use a TMA to make our live= easier. The TMA contains known positives and known negatives. I=n cases of t-cell or b-cell markers or cytokeratins. 25 to 30 cases i= easy. But when you are validated for more hard to find markers (SV-=0) then fewer cases is acceptable. We always throw in a slide that w= know will not stain for sv-40 like a tonsil - then you can say it has spe=ificity. Any inspector that I have come across is usually understanding=f this. But I am sure that there are exceptions to this.........esp ecially if they are not familiar with immunohistochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong M=morial Hospital Department of Surgical Pathology (585) 275-7210 ______________________ 5F__=5F______________ From: histonet-bounces@lis= ts.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf= Of thisisann@aol.com [thisisann@aol.com] Sent: Wednesday, April 28, 201= 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] R=sponses to IHC CAP Validation question The following is one respone= rec'd: 1. I asked CAP who told me that they do not currentl= have a guideline on validating but that they recommend what is in t=e following book: Quality Management In Anatomic Pathology, Promoting P=tient Safety Through Systems Improvement and Error by Raouf E. Nakhl=h, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8-=uality Management in IHC That is what we follow. I. Get a new antib=dy and optimize it with your positive control. II. Once optimized you n=ed to run it on cases expected to be positive (how many?) "a suffien= size ..." III. Must also be run on cases expected to be negative. (how=any? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just =hat. (ex. some of the hormones we just use a pituitary) ___________________ ______________________ 5F__=5F__ Histonet mailing list Histonet@lists.utsouthwestern.edu =2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________ 5F__ ______________________ Histo=et mailing list Histonet@lists.utsouthwestern.edu [3]http://l=sts.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:tonia.richmond@gracepathology.com" 2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 3. 3D"http://= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Thu May 6 12:43:12 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu May 6 12:43:18 2010 Subject: [Histonet] automated special stain platforms Message-ID: <24A4826E8EF0964D86BC5317306F58A54258D3843C@mmc-mail.ad.mhsil.com> We have been using a particular special stain platform for our automated special stains but are in the process of reevaluating. Can anybody share with me what instrumentation they prefer and are using? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From chak_bou <@t> yahoo.com Thu May 6 12:45:14 2010 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Thu May 6 12:45:18 2010 Subject: [Histonet] Campylobacter Jejuni Message-ID: <124608.42135.qm@web58105.mail.re3.yahoo.com> Hi Histonet, Is there anyone using Campylobacter antibody? If so, can you tell me where did you buy it? can you share the protocol? Any input will be appreciated! Thank you so much Chakib HTL( ASCP) MIT-DCM From gu.lang <@t> gmx.at Thu May 6 12:54:03 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu May 6 12:54:27 2010 Subject: AW: [Histonet] Formalin fixation time for breast specimens In-Reply-To: <4BE2AEB7.7400.0077.1@harthosp.org> References: <4BE2AEB7.7400.0077.1@harthosp.org> Message-ID: <345DE2350F584B95B3FDCF0671C3F7D4@dielangs.at> I've read only the abstract and some infos regarding this article in the internet. This group used a short processing protocol (2:30), didn't they? I think that hampers the comparability with standard overnight processing. And I missed the longer fixation tests. They mentioned, that Goldstein left the tissue in 100% cold ethanol before processing. I assume, that ethanol-fixation is the cause for weaker staining in underfixed areas. How are the commonly known edge-effects in ER-staining with stronger staining in the outer areas to explain, if not with insufficient fixation? It could be possible, that the longer incubation with processing reagents has effects on underfixed areas, that were not obvious in their experiment because of the short processing. Hm, I'm not convinced. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Richard Cartun Gesendet: Donnerstag, 06. Mai 2010 17:58 An: Histonet Betreff: [Histonet] Formalin fixation time for breast specimens There has been a lot of discussion recently regarding recommendations for formalin fixation of breast specimens. If you are interested in this topic please read the following article published in the May 2010 issue of the American Journal of Clinical Pathology by Ibarra JA, et al., "Fixation time does not affect the expression of estrogen receptor". Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maggie.allen <@t> nicewareintl.com Thu May 6 13:26:55 2010 From: maggie.allen <@t> nicewareintl.com (Maggie Allen) Date: Thu May 6 13:27:00 2010 Subject: [Histonet] RE: Barcode and Tracking Information In-Reply-To: <926351C712074D27B3AC517297DF0A72@your4105e587b6> References: <20100503170030.9B5688FC4A@spamfilter1.redanvil.net><6BCD4D0894AE0A4A96B4CB8F6779A57001874743@NICEWARE-MAIN03.NicewareIntl03.local><6BCD4D0894AE0A4A96B4CB8F6779A57001874785@NICEWARE-MAIN03.NicewareIntl03.local> <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A157@EXCHMBC2.ad.ah.local> <926351C712074D27B3AC517297DF0A72@your4105e587b6> Message-ID: <6BCD4D0894AE0A4A96B4CB8F6779A5700187479B@NICEWARE-MAIN03.NicewareIntl03.local> For those interested in learning more about barcode and tracking within a histology / anatomic pathology lab and how LabelClinic HTS can help, please feel free to join us next Wednesday at 3:00PM EST for a free overview of the entire LabelClinic HTS system. To register simply follow this link: https://nicewareintl.webex.com/nicewareintl/j.php?ED=117135017&RG=1&UID=1045276972&RT=MiMxMQ%3D%3D LabelClinic HTS is LIS agnostic and can also be used as a stand alone system. We have the ability to drive all printing devices for the identification of slides and cassettes so are hardware agnostic as well. The system is completely configurable to meet each labs particular needs and offers an abundance of functionality, validation, verification, tracking and reporting. Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel? (810) 629-3930 Cell (215) 200-0268 Corporate Numbers : General: (262) 784-2456 Toll Free: (888) 894-NICE (6423) Fax: (262) 784-2495 Technical Support: (262) 784-2466 Email: maggie.allen@nicewareintl.com www.nicewareintl.com http://healthcare.nicewareintl.com FREE Webinars : Friday, May 7th, 2010 10:30-11:30am CDT NiceLabel Print Center Review (Register) Friday, May 11th, 2010 01:00 - 02:00pm CDT Free NiceLabel Pro Design Training Session (Register) Friday, May 21st, 2010 10:30-11:30am CDT A Technical Review of the Enterprise Print Manager (Register) "The information in this e-mail and any attachments is confidential and may be subject to legal professional privilege. It is intended solely for the attention and use of the named addressee(s). If you are not the intended recipient or person responsible for delivering this information to the intended recipient, please notify the sender immediately. Unless you are the intended recipient or his/her representative you are not authorized to, and must not, read, copy, distribute, use or retain this message or any part of it" -----Original Message----- From: Rae Staskiewicz [mailto:raestask@grics.net] Sent: Wednesday, May 05, 2010 6:33 PM To: 'Mahoney,Janice A'; Maggie Allen; histonet@lists.utsouthwestern.edu Cc: histonet-request@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Barcode and Tracking Information Jan, Ditto! Ditto! Ditto! Couldn't have said it better myself! Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, May 05, 2010 2:53 PM To: 'Maggie Allen'; histonet@lists.utsouthwestern.edu Cc: histonet-request@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Blake, I think I put in my comments about Ventana's Vantage when you first posted the question, but here goes again.. It is wonderful. Vantage is the best thing to come along in my lifetime as a histo tech! Wonderful for patient safety, easy for the techs to use and a manager's dream for all the data it can provide. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maggie Allen Sent: Wednesday, May 05, 2010 2:24 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Hello Blake, LabelClinic HTS is a tracking system for Histology artifacts within the Pathology workflow. The product is designed to provide improved quality control and traceability with the lab. It provides flexible and reliable labeling and tracking of histology requisitions, containers, specimens, blocks and slides. Using bar code technology, LabelClinic HTS provides an optimized workflow that reduces errors and significantly enhances patient safety. It can be used stand alone, or integrated into an LIS / AP software system. Benefits: * Flexible lab configuration for custom lab workflow * Reduce errors and increase efficiency * Just-in-time reporting of artifact locations or last know location * Alerting for sub-process time violations and missing artifacts * Deploy to Desktop PC's or mobile hand held's * Configurable workflow * Supports color selection for slide and cassette printers I would be happy to set up an online WebEx demo of the system for anyone who may be interested. Thank you! Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel (810) 629-3930 Cell (215) 200-0268 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu May 6 13:38:42 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu May 6 13:39:05 2010 Subject: [Histonet] Responses to IHC CAP Validation question In-Reply-To: References: <8CCB51A55105D80-1A38-1C45@webmail-m088.sysops.aol.com>, Message-ID: <1AAF670737F193429070841C6B2ADD4C018766A4C1@EXMBMCB15.ucsfmedicalcenter.org> Tonia, obviously you have to validate your systems so you need to do some validation ahead of time with non-patient samples. You can validate your instruments and reagents on tissue arrays (you can even get commercial validated arrays for ER, PR and Her2). I suggest Pantomics (www.pantomics.com) for excellent quality arrays of all kinds. To validate the actual test, once you start up you will have to do some parallel testing by testing in-house and sending paired samples out to be tested. Then document the results of each. When you can show concordance of results over a set of samples (for most antibodies, 10 or more, 5 pos and 5 negative) that test can be considered validated. For ER, PR and Her2 you will need to have concordance on many more samples (25 - 100). I suggest, if possible, taking extra tissue from patient samples and making either tissue arrays or sausage arrays to do this testing. It will drastically lower the number of slides you have to test in house and to send out to be tested. It may be possible to partner with another lab to do this testing. As part of proficiency testing you can ask other labs for samples (wet tissue or unstained slides) to validate your results against theirs. Some labs are looking for partners to do this kind of testing. You then each validate each others tests. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tonia.richmond@gracepathology.com Sent: Thursday, May 06, 2010 8:28 AM To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu; thisisann@aol.com Subject: RE: [Histonet] Responses to IHC CAP Validation question If you are a brand new lab, how do you validate IHC if you are no= yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer =aboratory Grace Pathology PH: (501) 765-7367 Email: =]tonia.=chmond@gracepathology.com -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- <=ONT> To: "thisisann@=l.com" , "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" Sent by: histonet-bounces@lists.u=outhwestern.edu Date: 04/28/2010 02:01PM Subject: RE: [Histonet] Re=onses to IHC CAP Validation question Any inspection that I have under=ne we have used the 25 to 30 case rule. Except for the Er/Pr//Her-2=e use closer to 50 cases. We also use a TMA to make our live=asier. The TMA contains known positives and known negatives. I=ases of t-cell or b-cell markers or cytokeratins. 25 to 30 cases i=asy. But when you are validated for more hard to find markers (SV-@) then fewer cases is acceptable. We always throw in a slide that w=now will not stain for sv-40 like a tonsil - then you can say it has spe=ficity. Any inspector that I have come across is usually understanding= this. But I am sure that there are exceptions to this.........esp ecially if they are not familiar with immunohistochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong M=orial Hospital Department of Surgical Pathology (585) 275-7210 ______________________ 5F__=F______________ From: histonet-bounces@lis=.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf= thisisann@aol.com [thisisann@aol.com] Sent: Wednesday, April 28, 201:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] R=ponses to IHC CAP Validation question The following is one respone=ec'd: 1. I asked CAP who told me that they do not currentl=ave a guideline on validating but that they recommend what is in t= following book: Quality Management In Anatomic Pathology, Promoting P=ient Safety Through Systems Improvement and Error by Raouf E. Nakhl=, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8-=ality Management in IHC That is what we follow. I. Get a new antib=y and optimize it with your positive control. II. Once optimized you n?d to run it on cases expected to be positive (how many?) "a suffien=ize ..." III. Must also be run on cases expected to be negative. (how=ny? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just =at. (ex. some of the hormones we just use a pituitary) ___________________ ______________________ 5F__=F__ Histonet mailing list Histonet@lists.utsouthwestern.edu =]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________ 5F__ ______________________ Histo=t mailing list Histonet@lists.utsouthwestern.edu [3]http://l=ts.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:tonia.richmond@gracepathology.com" 2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 3. 3D"http://= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu May 6 13:40:19 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu May 6 13:40:23 2010 Subject: [Histonet] Sticky knives Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E470B5@nmdamailsvr.nmda.ad.nmsu.edu> Some time back, I posted a message here about how my Feather blades were sticking together and hard to dispense. A nice Sakura person contacted me to offer help but I'd just opened a new dispenser of blades and they were just peachy, so I replied to her that all was well. Well, not entirely true - could that nice Sakura rep please contact me again so I can discuss her offer of help? Thank you. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From c.m.vanderloos <@t> amc.uva.nl Thu May 6 13:53:46 2010 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu May 6 13:54:06 2010 Subject: [Histonet] RE: CD133 Message-ID: <9beb45742fff6a47.4be32c5a@amc.uva.nl> Claire, I don't know where to send your slides to, but do know a good CD133 antibody though. Go to the Human Protein Atlas website (www.proteinatlas.org) to see the staining patterns of several normal human tissues. CD133 is available through Sigma Prestige antibodies.Hope this helps, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Wed, 5 May 2010 22:15:03 -0500 From: "Ingles Claire " Subject: [Histonet] CD 133 To: histonet@lists.utsouthwestern.edu Thought I'd try again... Anyone know where I can send some slides for a CD133? Claire From histotech <@t> imagesbyhopper.com Thu May 6 14:13:04 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu May 6 14:13:05 2010 Subject: [Histonet] automated special stain platforms In-Reply-To: <24A4826E8EF0964D86BC5317306F58A54258D3843C@mmc-mail.ad.mhsil.com> Message-ID: <9BEBFB7A2A4F4FD49B23FD62D6DB99C6@hopperPC> We are using the Ventana Nexus special stainer and really like the results. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, May 06, 2010 1:43 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] automated special stain platforms We have been using a particular special stain platform for our automated special stains but are in the process of reevaluating. Can anybody share with me what instrumentation they prefer and are using? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2852 - Release Date: 05/05/10 18:26:00 From rgrow <@t> bmnet.com Thu May 6 14:27:26 2010 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Thu May 6 14:27:30 2010 Subject: [Histonet] Renee Grow is out of the office. Message-ID: I will be out of the office starting 05/06/2010 and will not return until 05/18/2010. I will respond to your message when I return. ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. From Timothy.Morken <@t> ucsfmedctr.org Thu May 6 14:39:14 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu May 6 14:39:32 2010 Subject: [Histonet] Formalin fixation time for breast specimens In-Reply-To: <4BE2AEB7.7400.0077.1@harthosp.org> References: <4BE2AEB7.7400.0077.1@harthosp.org> Message-ID: <1AAF670737F193429070841C6B2ADD4C018766A549@EXMBMCB15.ucsfmedicalcenter.org> Nice wrench, eh? They point out that they used known high expressing tumors to do this experiment. High expressors will stain with almost anything, even poor AR or weak antibody. So, the question has to be: what about low expressors? They conclude that the fixation paradigm should be rethought (now 6 - 72 hours), but I don't think the experiment is complete without testing the full range of expression. Oyama, et al (Breast Cancer 14:182-188, 2007) was experimenting with over-fixation (up to 3 weeks) but started at 3 hours as the "under fixation" for comparison. In the one low-expressing case his group tested they got negative (Allred score) at 3 hours fixation, +2 Allred at 6 hours and +3 Allred at 24 hours. But for higher expressors (6 Allred and up) they also found that there was either no difference or a slight increase in Allred score when fixation was 3, 6 or 24 hours, or even 3 weeks. Considering that pathologists will often score ER as positive when the percentage score is between 1% to 10% expression, that does not leave much room for error if under-fixation causes false negatives. And this paper does not even mention the parallel testing that is normally done on the same tissue block for PgR and Her2. These days that should be factored into any discussion about fixation of breast samples. Or shall fix a different sample for each test (may happen!!). They also used a different processing method (rapid sakura microwave) than the method used in the papers they compare against. Not necessarily bad, but they did not control for the different methods with parallel testing. They also did not control for their criticism of another paper storing samples in 100% alcohol after fixation, but before processing (to enable processing all samples at once). I'm not aware of any evidence either way that storage in 100% alcohol up to 7 days will affect ER staining. These authors imply that it did affect staining. I suggest reading the accompanying editorial by Neal Goldstein after reading the paper itself. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, May 06, 2010 8:58 AM To: Histonet Subject: [Histonet] Formalin fixation time for breast specimens There has been a lot of discussion recently regarding recommendations for formalin fixation of breast specimens. If you are interested in this topic please read the following article published in the May 2010 issue of the American Journal of Clinical Pathology by Ibarra JA, et al., "Fixation time does not affect the expression of estrogen receptor". Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shshaw <@t> WPI.EDU Thu May 6 15:52:25 2010 From: shshaw <@t> WPI.EDU (Shaw, Sharon) Date: Thu May 6 15:52:30 2010 Subject: [Histonet] refurbished equipment Message-ID: Hello everyone, Vendors and sales reps please don't call my office and leave messages it is a shared phone and this is an opportunity outside my current position please leave me an email and I will get back to you. Many Thanks, Sharon From saby_joseph_a <@t> yahoo.com Thu May 6 17:01:22 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Thu May 6 17:01:27 2010 Subject: [Histonet] Sticky knives In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E470B5@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E470B5@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <911985.3308.qm@web114407.mail.gq1.yahoo.com> Sara- I just recently had a box of sticky blades.? In fact, there was no way to pry one loose. However, some kind soul on the Histonet had posted that a few drops of microtome (or sewing machine) oil will loosen the blades when stuck like that. The effect may not be instantaneous, but it does indeed work. Good luck! Joe Saby, BA HT ________________________________ From: "Breeden, Sara" To: histonet@lists.utsouthwestern.edu Sent: Thu, May 6, 2010 2:40:19 PM Subject: [Histonet] Sticky knives Some time back, I posted a message here about how my Feather blades were sticking together and hard to dispense.? A nice Sakura person contacted me to offer help but I'd just opened a new dispenser of blades and they were just peachy, so I replied to her that all was well.? Well, not entirely true - could that nice Sakura rep please contact me again so I can discuss her offer of help?? Thank you. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mdeguzman <@t> lifecell.com Fri May 7 07:00:25 2010 From: mdeguzman <@t> lifecell.com (DeGuzman, Maria) Date: Fri May 7 07:00:36 2010 Subject: [Histonet] Special stain to demonstrate Silver In-Reply-To: <3c1ed7b7-cb37-40cc-869c-6cb7136c7240@amwpht02.kci.com> References: <3c1ed7b7-cb37-40cc-869c-6cb7136c7240@amwpht02.kci.com> Message-ID: <5476245379016B4D8212E8BCD11ECFFBB77A5C68@AMWPVEX01.kci.com> Hello Histoneters, Is there a special stain to detect silver in tissue? If there is please share the procedure. Thanks, Fanny Maria V. De Guzman| Histology Technician I Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1482 Email mdeguzman@lifecell.com Mobile 732.688.1386 www.Lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, May 06, 2010 2:16 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. clo test (Tench, Bill) 2. IDEA FOR A NEW RECYCLER!!!! (Madary, Joseph) 3. RE: RE: Barcode and Tracking Information (Rae Staskiewicz) 4. CD 133 (Ingles Claire ) 5. Disposable blade holder - amateur microscopist looking for a cheap one! (Gordon Brown) 6. Disposable blade holder - amateur microscopist looking for a cheap one! (Gordon Brown) 7. NSH Region II Meeting-discount hotel rate deadline is coming up! (Goodwin, Diana) 8. dehydration of hydrated slide.... (Eva Permaul) 9. RE: dehydration of hydrated slide.... (Sebree Linda A) 10. RE: dehydration of hydrated slide.... (Mauger, Joanne) 11. B5 fixative (histotech@imagesbyhopper.com) 12. myocyte damage (Bartlett, Jeanine (CDC/OID/NCZVED)) 13. RE: Responses to IHC CAP Validation question (tonia.richmond@gracepathology.com) 14. Starting up new lab (Shaw, Sharon) 15. Formalin fixation time for breast specimens (Richard Cartun) 16. RE: Responses to IHC CAP Validation question (BSullivan@shorememorial.org) 17. Re: myocyte damage (Merced M Leiker) ---------------------------------------------------------------------- Message: 1 Date: Wed, 5 May 2010 13:57:20 -0700 From: "Tench, Bill" Subject: [Histonet] clo test To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863055@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii The Clo test is a clinical lab test. You need to go to that part of the CPT coding book (sorry I don't have it available). 88300 is an anatomic code (gross only, ie, it requires examination of a piece of tissue or foreign body) and is entirely inappropriate for this test. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, May 05, 2010 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 78, Issue 6 Hi everyone, I am looking to see what CPT code everyone is using for reading Clo Tests in the pathology department. I have heard of using 87081 but I am not sure if this is accurate as this is for culture and the CLO is biochemical reaction not a culture. Currently I have been using 88300 gross only. Any help would be appreciated. Thank you, Amy Farnan *************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 2 Date: Wed, 5 May 2010 17:22:22 -0400 From: "Madary, Joseph" Subject: [Histonet] IDEA FOR A NEW RECYCLER!!!! To: Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1301A37FFA@MD1EV002.medimmune.com> Content-Type: text/plain; charset="us-ascii" Some manufacturer should find a way where you can add all of the chemicals together in one container and when the recycler runs it spits out formalin in one, xylene/hemo-de/f83/ in another and alcohol in another all from one run. To opine on another histnet query from earlier I think both have their place. I use CBG for xylene, form, hemo de and alcohol using the hemo on the processor and depar, alcohol on processor for low grade alc, and xyle for all but the last xylen on the stainer using frsh for that. Creative waste is good and simple but the only thing is mixing old and new formalin can't be good long term. What I was thinking about doing was using creative waste gravimetric for the first run, and then after that use the CBG to redistill NBF for round 2, 3, 4 etc. Hey where am I getting all this money and space? Still like BR recycler too, just do not have one anymore, but liked it. All recycling is good as long as people know which ones to use. Still seems to be an issue for some people. I worked in a place where the techs thought you could throw everyting in one container and the rcycler would spit out clean alcohol, xylene and formalin from one collective run. Hey manufactures? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ------------------------------ Message: 3 Date: Wed, 5 May 2010 17:32:47 -0500 From: "Rae Staskiewicz" Subject: RE: [Histonet] RE: Barcode and Tracking Information To: "'Mahoney,Janice A'" , "'Maggie Allen'" , Cc: histonet-request@lists.utsouthwestern.edu, ":histonet-bounces"@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <926351C712074D27B3AC517297DF0A72@your4105e587b6> Content-Type: text/plain; charset="us-ascii" Jan, Ditto! Ditto! Ditto! Couldn't have said it better myself! Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, May 05, 2010 2:53 PM To: 'Maggie Allen'; histonet@lists.utsouthwestern.edu Cc: histonet-request@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Blake, I think I put in my comments about Ventana's Vantage when you first posted the question, but here goes again.. It is wonderful. Vantage is the best thing to come along in my lifetime as a histo tech! Wonderful for patient safety, easy for the techs to use and a manager's dream for all the data it can provide. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maggie Allen Sent: Wednesday, May 05, 2010 2:24 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Hello Blake, LabelClinic HTS is a tracking system for Histology artifacts within the Pathology workflow. The product is designed to provide improved quality control and traceability with the lab. It provides flexible and reliable labeling and tracking of histology requisitions, containers, specimens, blocks and slides. Using bar code technology, LabelClinic HTS provides an optimized workflow that reduces errors and significantly enhances patient safety. It can be used stand alone, or integrated into an LIS / AP software system. Benefits: * Flexible lab configuration for custom lab workflow * Reduce errors and increase efficiency * Just-in-time reporting of artifact locations or last know location * Alerting for sub-process time violations and missing artifacts * Deploy to Desktop PC's or mobile hand held's * Configurable workflow * Supports color selection for slide and cassette printers I would be happy to set up an online WebEx demo of the system for anyone who may be interested. Thank you! Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel (810) 629-3930 Cell (215) 200-0268 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 5 May 2010 22:15:03 -0500 From: "Ingles Claire " Subject: [Histonet] CD 133 To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Thought I'd try again... Anyone know where I can send some slides for a CD133? Claire ------------------------------ Message: 5 Date: Thu, 6 May 2010 09:16:36 +0100 From: "Gordon Brown" Subject: [Histonet] Disposable blade holder - amateur microscopist looking for a cheap one! To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I dabble in microscopy - well I say I dabble, my wife says otherwise! - and I have a reasonably well equipped home lab, which includes the ubiquitous Cambridge Rocker. I've had reasonably good results with plant tissue sections but sharpening the blades has always proved to be a pain and I tend to get mixed results. However, I've recently acquired a bunch of Accu Edge low profile blades at very low cost but I'm unable to source a holder at an affordable price. IS there anyone out there in professional histology land who has for sale a used, even rough condition holder suitable for these blades? You'd get the grateful thanks of both myself and my wife, who will be more than pleased to see me spend less time muttering about my poor sharpening skills! Many thanks Gordon (UK) ------------------------------ Message: 6 Date: Thu, 6 May 2010 09:36:41 +0100 From: "Gordon Brown" Subject: [Histonet] Disposable blade holder - amateur microscopist looking for a cheap one! To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Perhaps I should point out that despite the date in the UK, my name is genuinely as given, although my namesake may well be looking for a new hobby to fill in his spare time after today........... Gordon ------------------------------ Message: 7 Date: Thu, 6 May 2010 08:18:01 -0400 From: "Goodwin, Diana" Subject: [Histonet] NSH Region II Meeting-discount hotel rate deadline is coming up! To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="windows-1252" You don?t want to miss the upcoming Region II Meeting on June 10-12 in Atlantic City NJ! 25 speakers are presenting 30 different topics from wet workshops to short seminars. CEUs will be granted for all sessions. Over 30 vendors are participating in our Exhibit Area. Your registration fee includes free admission to the Vendor Exhibit, AM and PM Breaks, buffet lunch and Friday evening?s reception. The hotel room rate is reduced to $89/night, but you must reserve by Monday, May 10th! The mail-in registration deadline is May 20th. For more information, you can download a meeting brochure from the NSH website under state meetings at www.nsh.org/content/region-ii-meeting. ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA. ------------------------------ Message: 8 Date: Thu, 06 May 2010 08:56:01 -0400 From: Eva Permaul Subject: [Histonet] dehydration of hydrated slide.... To: histonet@lists.utsouthwestern.edu Message-ID: <4BE2BC61.2040701@georgetown.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University ------------------------------ Message: 9 Date: Thu, 6 May 2010 08:49:06 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] dehydration of hydrated slide.... To: "Eva Permaul" , Message-ID: <8C023B4AB999614BA4791BAEB26E2738399E3B@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="US-ASCII" Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 6 May 2010 11:02:59 -0400 From: "Mauger, Joanne" Subject: RE: [Histonet] dehydration of hydrated slide.... To: Sebree Linda A , Eva Permaul , "histonet@lists.utsouthwestern.edu" Message-ID: <443F5B475A9BF647AB962E834884EBAD2788770DE6@EX7CCRPW03V1.chop.edu> Content-Type: text/plain; charset="us-ascii" Eva, If you dehydrate it again to 100% or xylene, it is less likelt to fall off the slide. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A [LSebree@uwhealth.org] Sent: Thursday, May 06, 2010 9:49 AM To: Eva Permaul; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] dehydration of hydrated slide.... Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 6 May 2010 11:20:01 -0400 From: Subject: [Histonet] B5 fixative To: Message-ID: Content-Type: text/plain; charset="US-ASCII" I was under the impression that B5, because of the mercury content, was outlawed for use Jan 1, 2005. But now I am not so sure! Can anyone tell me if there is a federal law regarding this? We no longer use B5, we use B+, but I know someone in OK who is using B5 (I am in FL). Is he breaking any laws by using it and/or should he be switched to an alternative like B+? Thanks! Michelle ------------------------------ Message: 12 Date: Thu, 6 May 2010 11:16:04 -0400 From: "Bartlett, Jeanine (CDC/OID/NCZVED)" Subject: [Histonet] myocyte damage To: histonet Message-ID: <68510B12184E45498EABD4CB6F3868FE012870BC@LTA3VS001.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii Hello everyone, I am in need of a special stain or an IHC that will demonstrate myocyte damage in muscle tissue...esp. cardiac. Any help will be greatly appreciated. Thanks! Jeanine Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov ------------------------------ Message: 13 Date: Thu, 6 May 2010 10:27:50 -0500 From: tonia.richmond@gracepathology.com Subject: RE: [Histonet] Responses to IHC CAP Validation question To: "McMahon, Loralee A" Cc: "histonet@lists.utsouthwestern.edu" , "thisisann@aol.com" Message-ID: Content-Type: text/plain; charset="UTF-8" If you are a brand new lab, how do you validate IHC if you are no yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer Grace Pathology PH: (501) 765-7367 Email: -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- < To: "thisisann@ "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" Subject: [Histonet] Starting up new lab To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I'm looking at starting up a new histology lab and need everything, I have a tight budget. Can anybody give me recommendations on where to buy refurbished equipment. Thanks Sharon ------------------------------ Message: 15 Date: Thu, 06 May 2010 11:57:43 -0400 From: "Richard Cartun" Subject: [Histonet] Formalin fixation time for breast specimens To: "Histonet" Message-ID: <4BE2AEB7.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII There has been a lot of discussion recently regarding recommendations for formalin fixation of breast specimens. If you are interested in this topic please read the following article published in the May 2010 issue of the American Journal of Clinical Pathology by Ibarra JA, et al., "Fixation time does not affect the expression of estrogen receptor". Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 16 Date: Thu, 6 May 2010 12:16:45 -0400 From: BSullivan@shorememorial.org Subject: RE: [Histonet] Responses to IHC CAP Validation question To: tonia.richmond@gracepathology.com Cc: "histonet@lists.utsouthwestern.edu" , "McMahon, Loralee A" , histonet-bounces@lists.utsouthwestern.edu, "thisisann@aol.com" Message-ID: Content-Type: text/plain; charset=US-ASCII One important thing to remember is that you should make sure All material being used for validation is processed the same way. Will your control tissue be purchased or processed in your lab? Control tissue is used for validation but you should use tissue with various levels of positivity. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 tonia.richmond@gr acepathology.com Sent by: To histonet-bounces@ "McMahon, Loralee A" lists.utsouthwest cc "histonet@lists.utsouthwestern.edu" 05/06/2010 11:27 AM , "thisisann@aol.com" Subject RE: [Histonet] Responses to IHC CAP Validation question If you are a brand new lab, how do you validate IHC if you are no= t yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer = / Laboratory Grace Pathology PH: (501) 765-7367 Email: = [1]tonia.= richmond@gracepathology.com -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- <= /FONT> To: "thisisann@= aol.com" , "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" Sent by: histonet-bounces@lists.u= tsouthwestern.edu Date: 04/28/2010 02:01PM Subject: RE: [Histonet] Re= sponses to IHC CAP Validation question Any inspection that I have under= gone we have used the 25 to 30 case rule. Except for the Er/Pr//Her-2= . We use closer to 50 cases. We also use a TMA to make our live= s easier. The TMA contains known positives and known negatives. I= n cases of t-cell or b-cell markers or cytokeratins. 25 to 30 cases i= s easy. But when you are validated for more hard to find markers (SV-= 40) then fewer cases is acceptable. We always throw in a slide that w= e know will not stain for sv-40 like a tonsil - then you can say it has spe= cificity. Any inspector that I have come across is usually understanding= of this. But I am sure that there are exceptions to this.........esp ecially if they are not familiar with immunohistochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong M= emorial Hospital Department of Surgical Pathology (585) 275-7210 ______________________ 5F__= _______________ From: histonet-bounces@lis= ts.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf= Of thisisann@aol.com [thisisann@aol.com] Sent: Wednesday, April 28, 201= 0 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] R= esponses to IHC CAP Validation question The following is one respone= I rec'd: 1. I asked CAP who told me that they do not currentl= y have a guideline on validating but that they recommend what is in t= he following book: Quality Management In Anatomic Pathology, Promoting P= atient Safety Through Systems Improvement and Error by Raouf E. Nakhl= eh, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8-= Quality Management in IHC That is what we follow. I. Get a new antib= ody and optimize it with your positive control. II. Once optimized you n= eed to run it on cases expected to be positive (how many?) "a suffien= t size ..." III. Must also be run on cases expected to be negative. (how= many? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just = that. (ex. some of the hormones we just use a pituitary) ___________________ ______________________ 5F__= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu = [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________ 5F__ ______________________ Histo= net mailing list Histonet@lists.utsouthwestern.edu [3]http://l= ists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:tonia.richmond@gracepathology.com" 2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 3. 3D"http://=/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 06 May 2010 12:30:50 -0400 From: Merced M Leiker Subject: Re: [Histonet] myocyte damage To: "Bartlett, Jeanine (CDC/OID/NCZVED)" , histonet Message-ID: <5AFA0A6D83365A1F044D4E16@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Stain with cTnI and inspect for damage visually. Just a guess. Regards, Merced --On Thursday, May 06, 2010 11:16 AM -0400 "Bartlett, Jeanine (CDC/OID/NCZVED)" wrote: > Hello everyone, > > I am in need of a special stain or an IHC that will demonstrate myocyte > damage in muscle tissue...esp. cardiac. > > Any help will be greatly appreciated. > > Thanks! > Jeanine Bartlett > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 7 *************************************** From AHutton <@t> dh.org Fri May 7 09:20:00 2010 From: AHutton <@t> dh.org (Hutton, Allison) Date: Fri May 7 09:21:48 2010 Subject: [Histonet] proper disposal of chemicals Message-ID: <38A56C4F4630D348A50B3720409270870744FFB6@dhmail.dhorg.org> I have a fair amount of different chemicals that are expired and need to be disposed of. I am looking for advice on how to properly dispose of the following chemicals beyond "in accordance with local, state, and federal guidelines": *Gold Chloride 0.2% aq *Schiff reagent *Aniline Carbol Fuchsin *Gomori Trichrome Stain *Glycerol *Iodine Powder *Ammoniacal Silver Nitrate *1N Sodium Hydroxide Any advice would be greatly appreciated I am located in PA Thanks, Allison From mpowers <@t> dpspa.com Fri May 7 09:29:17 2010 From: mpowers <@t> dpspa.com (Marian Powers) Date: Fri May 7 09:29:52 2010 Subject: [Histonet] Reference Labs Message-ID: Hi: Is there anyone who can provide me some references/feedback on OncoDiagnostic Laboratories? Thanks, M.Seay -- From brett_connolly <@t> merck.com Fri May 7 10:09:41 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri May 7 10:09:47 2010 Subject: [Histonet] proper disposal of chemicals In-Reply-To: <38A56C4F4630D348A50B3720409270870744FFB6@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870744FFB6@dhmail.dhorg.org> Message-ID: <63EA0607835FBA4689CEA9EA8B482692030DC5A3@usctmx1141.merck.com> We use Clean Harbors http://www.cleanharbors.com/ and let them handle everything. Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Friday, May 07, 2010 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] proper disposal of chemicals I have a fair amount of different chemicals that are expired and need to be disposed of. I am looking for advice on how to properly dispose of the following chemicals beyond "in accordance with local, state, and federal guidelines": *Gold Chloride 0.2% aq *Schiff reagent *Aniline Carbol Fuchsin *Gomori Trichrome Stain *Glycerol *Iodine Powder *Ammoniacal Silver Nitrate *1N Sodium Hydroxide Any advice would be greatly appreciated I am located in PA Thanks, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From asmith <@t> mail.barry.edu Fri May 7 10:25:31 2010 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri May 7 10:26:34 2010 Subject: [Histonet] RE: proper disposal of chemicals In-Reply-To: <38A56C4F4630D348A50B3720409270870744FFB6@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870744FFB6@dhmail.dhorg.org> Message-ID: <70AC46F12D9EF2438760A84225B6BC101562DA74@EX2010-01.barrynet.barry.edu> Look up "Waste Hauling" in the Yellow Pages. Ask every company listed for a quote. Make sure the ammoniacal silver nitrate is wet; when it dries, it can rearrange to silver azide, a powerful and touchy primary explosive. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Friday, May 07, 2010 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] proper disposal of chemicals I have a fair amount of different chemicals that are expired and need to be disposed of. I am looking for advice on how to properly dispose of the following chemicals beyond "in accordance with local, state, and federal guidelines": *Gold Chloride 0.2% aq *Schiff reagent *Aniline Carbol Fuchsin *Gomori Trichrome Stain *Glycerol *Iodine Powder *Ammoniacal Silver Nitrate *1N Sodium Hydroxide Any advice would be greatly appreciated I am located in PA Thanks, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Montina.VanMeter <@t> pbrc.edu Fri May 7 10:53:57 2010 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Fri May 7 11:04:24 2010 Subject: [Histonet] 2010 Louisiana Society for Histotechnology Symposium/Conference Message-ID: <4FE7FB862E90E448AE32388E759220E50243B73F@pbrcas31.pbrc.edu> Hello Histonetters, The Louisiana Society for Histotechnology would like to invite you to our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA. Meeting location: The Embassy Suites Hotel 414 Constitution Ave. Baton Rouge, LA 70808 We have a block of rooms reserved for attendees at the special rate of $99.00. After the cut-off date of May13, 2010, the rooms may be reserved at that rate per availability. The hotel will honor this rate two days prior and two days after our meeting (please contact the Embassy Suites Hotel for further information: 1-225-924-6566 or 1-800-Embassy). A complimentary hotel shuttle service is provided for those flying into Baton Rouge. Great dining, beautiful southern plantations and gambling boats on the Mississippi River are just a few of the attractions in the Baton Rouge area. Remember to ask for the Louisiana Society for Histotechnology group when placing your reservation. Walk-ins are always welcome! If you have any questions please contact: Tina Van Meter at 225-603-0953 or vanmetmj@pbrc.edu. Workshops: WS #1: FISH - IT'S NOT JUST FOR DINNER! Bonnie Whitaker, HT (ASCP) - sponsored by Cell Marque OSU Medical Center WS #2: What is the Tumor Registry? Cynthia Boudreaux, LPN, CTR Touro Infirmary WS #3: Veterinary vs. Clinical - Which Career is Best for Me? Pam Marcum, B.S., M.S., HT, (ASCP) UAMS WS #4: Microtomy, It's About Technique! Mari Ann Mailhiot, BA, HT (ASCP) Leica Microsystems WS #5: Boot Camp for Histotechs Mari Ann Mailhiot, BS, HT (ASCP) Leica Microsystems WS #6: So You Want to Know More About Things Your Lab Doesn't Do Pam Marcum, B.S., M.S., HT (ASCP), Bonnie Whitaker, HT (ASCP) QIHC UAMS OSU Medical Center WS #7: Dilution, Titrations, and Good Pipetting in the IHC Laboratory Robin Simpkins, HT (ASCP) Biocare Medical WS #8: Detection Chemistry & Antibody Production Tania Ewing-Finchem, HT (ASCP) Ventana Roche Hope to see you in June! Tina LSH Secretary From gu.lang <@t> gmx.at Fri May 7 11:32:07 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri May 7 11:32:21 2010 Subject: [Histonet] Rhodanine method for silver Message-ID: <3ED79C0F8A9646D6A4B9119E01357D6C@dielangs.at> This procedure can be found in ?theory and practice of hitological techniques?, page 257. http://books.google.at/books?hl=de &lr=&id=Dhn2KispfdQC&oi=fnd&pg=PA233&dq=rhodanine+silver+pigment+okamoto&ots =JxpCewSzG8&sig=uF-Hxldt-U5dtVRhTWaaACpsf7k#v=onepage&q&f=false This e-book shows only the first side of the procedure, but some comments on silver pigments. On the next page in brief: Method: deparaffinize, rehydrate, incubate sections in rhodanin solution 37?C, 24 hours., wash well in destilled water, mount in glycerin jelly. Results: silver-deposits reddish brown. Section should be examined immidiatly, and some more notes in the book. Hope this helps Gudrun From kenneth.a.troutman <@t> Vanderbilt.Edu Fri May 7 11:46:41 2010 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri May 7 11:46:56 2010 Subject: [Histonet] Special stain to demonstrate Silver Message-ID: <7B310892042DA74CB3590053F424CFE60B83F96FF7@ITS-HCWNEM06.ds.Vanderbilt.edu> Hi Fanny, I don't know of one, but perhaps you could modify a GMS. I don't think you would need to oxidize it at the beginning with the periodic acid or chromic acid. Make the working Methenamine silver solution without the silver. (Don't forget to add the Borate) and try and visualize it that way. I probably wouldn't do the hypo and gold chloride until I looked at it under the scope to see if it was actually staining, you wouldn't want to remove the silver you are trying to visualize. I would be careful in interpreting that because I don't know what else it might be picking up--it might pick up other metals. That's just a suggestion off the cuff. If you try that, let us know what happens! Good luck! Thanks, Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 615-343-9134 Message: 15 Date: Fri, 7 May 2010 07:00:25 -0500 From: "DeGuzman, Maria" Subject: [Histonet] Special stain to demonstrate Silver To: "histonet@lists.utsouthwestern.edu" Message-ID: <5476245379016B4D8212E8BCD11ECFFBB77A5C68@AMWPVEX01.kci.com> Content-Type: text/plain; charset="us-ascii" Hello Histoneters, Is there a special stain to detect silver in tissue? If there is please share the procedure. Thanks, Fanny From ncosenza <@t> siumed.edu Fri May 7 12:27:29 2010 From: ncosenza <@t> siumed.edu (Nicole Cosenza) Date: Fri May 7 12:27:37 2010 Subject: [Histonet] DiD retrograde tracing study. Vibratome or cryostat Message-ID: <4BE44D81.2040801@siumed.edu> We are beginning a retrograde tracing study in rats. We injected DiD into the rats and in 4 weeks we will perfuse and harvest the brainstems. My question is, is vibratome sectioning better than cryostat sectioning? The literature that accompanied the dye said either is an option, but some have noted dye leaching out when cyrostat sectioned. How common is this problem? If vibratome is the better choice, is there a good embedding protocol? Nicole Southern Illinois University School of Medicine From TJJ <@t> stowers.org Fri May 7 12:36:42 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri May 7 12:36:52 2010 Subject: [Histonet] Re: Special stain to demonstrate silver Message-ID: Maria, I don't know of any particular procedure or stain to demonstrate silver, but I got to thinking wouldn't it reduce with formalin fixation to a visible state? Or maybe strong sunlight? Kind regards, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From srodriguez <@t> phenopath.com Fri May 7 12:47:51 2010 From: srodriguez <@t> phenopath.com (Stephanie Rodriguez) Date: Fri May 7 12:48:05 2010 Subject: [Histonet] Re: Histonet Digest, Vol 78, Issue 8 Message-ID: Tonia, If you are interested in sending specimens out for validation, our laboratory currently provides this service for HER2 testing by IHC and FISH. Information is on our website at www.phenopath.com, under ?Diagnostic Services? at the top of the home page. Good luck! Stephanie Rodriguez, BS, HTL(ASCP), QIHC Senior Molecular Technologist, FISH IHC Technologist III Phenopath Laboratories Seattle, WA On 5/7/10 5:47 AM, "histonet-request@lists.utsouthwestern.edu" wrote: > Message: 7 > Date: Thu, 6 May 2010 11:38:42 -0700 > From: "Morken, Tim" > Subject: RE: [Histonet] Responses to IHC CAP Validation question > To: "tonia.richmond@gracepathology.com" > , "McMahon, Loralee A" > > Cc: "histonet@lists.utsouthwestern.edu" > , "thisisann@aol.com" > > Message-ID: > <1AAF670737F193429070841C6B2ADD4C018766A4C1@EXMBMCB15.ucsfmedicalcenter.org> > > Content-Type: text/plain; charset=utf-8 > > Tonia, obviously you have to validate your systems so you need to do some > validation ahead of time with non-patient samples. You can validate your > instruments and reagents on tissue arrays (you can even get commercial > validated arrays for ER, PR and Her2). I suggest Pantomics (www.pantomics.com) > for excellent quality arrays of all kinds. > > To validate the actual test, once you start up you will have to do some > parallel testing by testing in-house and sending paired samples out to be > tested. Then document the results of each. When you can show concordance of > results over a set of samples (for most antibodies, 10 or more, 5 pos and 5 > negative) that test can be considered validated. For ER, PR and Her2 you will > need to have concordance on many more samples (25 - 100). I suggest, if > possible, taking extra tissue from patient samples and making either tissue > arrays or sausage arrays to do this testing. It will drastically lower the > number of slides you have to test in house and to send out to be tested. > > It may be possible to partner with another lab to do this testing. As part of > proficiency testing you can ask other labs for samples (wet tissue or > unstained slides) to validate your results against theirs. Some labs are > looking for partners to do this kind of testing. You then each validate each > others tests. > > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > tonia.richmond@gracepathology.com > Sent: Thursday, May 06, 2010 8:28 AM > To: McMahon, Loralee A > Cc: histonet@lists.utsouthwestern.edu; thisisann@aol.com > Subject: RE: [Histonet] Responses to IHC CAP Validation question > > > If you are a brand new lab, how do you validate IHC if you are no= > yet receiving patient specimens? Can the validation be done on cont rol > tissues only? > > > > > Sincerely, > > > Tonia Richmond, AS, HT (ASCP) > Chief Operations Officer =aboratory > Grace Pathology > PH: (501) 765-7367 > Email: =]tonia.=chmond@gracepathology.com This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From jcampbell <@t> vdxpathology.com Fri May 7 14:43:27 2010 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Fri May 7 14:43:31 2010 Subject: [Histonet] nonspecific staining in liver Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF838706C@VDXSERVER01.vdxpathology.local> Hi All, I am having some problems with marked nonspecific staining in liver when running my CD3 and CD79a. I am using an avidin and biotin block, as well as a protein block. Any other suggestions as to what I could do to eliminate this? Thanks in advance, Jennifer Campbell From jphistology <@t> gmail.com Fri May 7 16:50:18 2010 From: jphistology <@t> gmail.com (Joao Pessoa) Date: Fri May 7 16:50:23 2010 Subject: [Histonet] IgG4 source Message-ID: I need to find a source for an IgG4 antibody for use with IHC studies on human FFPE tissue. Any ideas? My preference would be for a concentrated IVD. Thanks, Joao Histo Tech From gordon <@t> 10db.co.uk Sat May 8 02:56:21 2010 From: gordon <@t> 10db.co.uk (Gordon Brown) Date: Sat May 8 02:56:34 2010 Subject: [Histonet] Disposable blade holder - amateur microscopist has now been offered one! Message-ID: An exceptionally kind member of the list has now offered me a blade holder that sounds ideal, many, many thanks to him and also to those who sent nice messages! One of the emails I received was from a lady who teaches histology who was interested in the Cambridge Rocker that I have and she asked if I could send some photos for teaching purposes. I was very happy to oblige her and I was also able to provide a copy of the instruction manual, which I have never seen online. As I have little to offer the list - other than questions! - I have given below links to both the photos and the pdf of the instructions in case there is anyone else out there who can make use of them. They've been posted to my webspace as they are HUGE files, the photos being taken using an Olympus DSLR and uploaded as full resolution jpgs. Gordon www.10db.co.uk/CRM_instructions.pdf www.10db.co.uk/CRM1.jpg www.10db.co.uk/CRM2.jpg www.10db.co.uk/CRM3.jpg www.10db.co.uk/CRM4.jpg www.10db.co.uk/CRM5.jpg www.10db.co.uk/CRM6.jpg www.10db.co.uk/CRM7.jpg www.10db.co.uk/CRM8.jpg www.10db.co.uk/CRM9.jpg From sheila_adey <@t> hotmail.com Sat May 8 09:17:29 2010 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Sat May 8 09:17:34 2010 Subject: [Histonet] (no subject) Message-ID: Hello everyone, Usually our Twort's method for the Gram stain works great. But recently we can't get the gram negatives to stain??? any ideas. Thanks in advance Sheila Adey HT _________________________________________________________________ Win $10,000 from Hotmail! Enter Here. http://go.microsoft.com/?linkid=9729708 From bhewlett <@t> cogeco.ca Sat May 8 11:58:41 2010 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Sat May 8 11:59:11 2010 Subject: [Histonet] Disposable blade holder - amateur microscopist has nowbeen offered one! References: Message-ID: Gordon, Thank you for posting the instructions and photos! They brought back memories of my student days back in the fifties. I can recall that one of my colleagues almost lost fingers when the piano wire broke as he was changing a specimen stub. Many hours of microtomy practice and even more hours spent hand sharpening the Heiffor knives!! I still have two original Wilkinson sword knives in my possession, although they are a little worse of wear now. Once again, thanks for the memories. Bryan Hewlett (retired histotechnologist) ----- Original Message ----- From: "Gordon Brown" To: Sent: Saturday, May 08, 2010 3:56 AM Subject: [Histonet] Disposable blade holder - amateur microscopist has nowbeen offered one! > An exceptionally kind member of the list has now offered me a blade holder > that sounds ideal, many, many thanks to him and also to those who sent > nice > messages! One of the emails I received was from a lady who teaches > histology > who was interested in the Cambridge Rocker that I have and she asked if I > could send some photos for teaching purposes. I was very happy to oblige > her > and I was also able to provide a copy of the instruction manual, which I > have never seen online. As I have little to offer the list - other than > questions! - I have given below links to both the photos and the pdf of > the > instructions in case there is anyone else out there who can make use of > them. They've been posted to my webspace as they are HUGE files, the > photos > being taken using an Olympus DSLR and uploaded as full resolution jpgs. > > Gordon > > www.10db.co.uk/CRM_instructions.pdf > www.10db.co.uk/CRM1.jpg > www.10db.co.uk/CRM2.jpg > www.10db.co.uk/CRM3.jpg > www.10db.co.uk/CRM4.jpg > www.10db.co.uk/CRM5.jpg > www.10db.co.uk/CRM6.jpg > www.10db.co.uk/CRM7.jpg > www.10db.co.uk/CRM8.jpg > www.10db.co.uk/CRM9.jpg > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jpastor1 <@t> nycap.rr.com Sat May 8 14:31:38 2010 From: jpastor1 <@t> nycap.rr.com (Joseph N. Pastore) Date: Sat May 8 14:31:40 2010 Subject: [Histonet] Loss of tissue sections after antigen retrieval Message-ID: Hi All, We have a problem. We are using a commercial tissue array and we find that the two most important tissues are lost during antigen retrieval. The tissues are samples of glioblastomas and are critical to our study. The antigen retrieval system we use is a proprietary 2 step system using a hi and lo pH. Both Ca chelators. Does anyone have suggestions about how we can prevent tissue loss???? From histotech <@t> imagesbyhopper.com Sat May 8 16:45:12 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat May 8 16:45:16 2010 Subject: [Histonet] Disposable blade holder - amateur microscopist hasnowbeen offered one! In-Reply-To: Message-ID: <636629AE52524EB1B2C325FC8107F5EF@hopperPC> WOW! And here I was thinking that my old, black, cast iron AO microtome was from the stone age! ;o) Gordon, thank you for sharing the photos and user guide - it was quite enlightening! Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Hewlett Sent: Saturday, May 08, 2010 12:59 PM To: gordon@10db.co.uk; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Disposable blade holder - amateur microscopist hasnowbeen offered one! Gordon, Thank you for posting the instructions and photos! They brought back memories of my student days back in the fifties. I can recall that one of my colleagues almost lost fingers when the piano wire broke as he was changing a specimen stub. Many hours of microtomy practice and even more hours spent hand sharpening the Heiffor knives!! I still have two original Wilkinson sword knives in my possession, although they are a little worse of wear now. Once again, thanks for the memories. Bryan Hewlett (retired histotechnologist) ----- Original Message ----- From: "Gordon Brown" To: Sent: Saturday, May 08, 2010 3:56 AM Subject: [Histonet] Disposable blade holder - amateur microscopist has nowbeen offered one! > An exceptionally kind member of the list has now offered me a blade > holder that sounds ideal, many, many thanks to him and also to those > who sent nice messages! One of the emails I received was from a lady > who teaches histology > who was interested in the Cambridge Rocker that I have and she asked if I > could send some photos for teaching purposes. I was very happy to oblige > her > and I was also able to provide a copy of the instruction manual, which I > have never seen online. As I have little to offer the list - other than > questions! - I have given below links to both the photos and the pdf of > the > instructions in case there is anyone else out there who can make use of > them. They've been posted to my webspace as they are HUGE files, the > photos > being taken using an Olympus DSLR and uploaded as full resolution jpgs. > > Gordon > > www.10db.co.uk/CRM_instructions.pdf > www.10db.co.uk/CRM1.jpg > www.10db.co.uk/CRM2.jpg > www.10db.co.uk/CRM3.jpg > www.10db.co.uk/CRM4.jpg > www.10db.co.uk/CRM5.jpg > www.10db.co.uk/CRM6.jpg > www.10db.co.uk/CRM7.jpg > www.10db.co.uk/CRM8.jpg > www.10db.co.uk/CRM9.jpg > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2852 - Release Date: 05/08/10 06:26:00 From pruegg <@t> ihctech.net Sat May 8 18:10:41 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat May 8 18:11:20 2010 Subject: SPAM-LOW: [Histonet] Loss of tissue sections after antigen retrieval In-Reply-To: References: Message-ID: <74FC819FCC4D439B96D37E23642D67C9@prueggihctechlt> Joe, Did you buy an array that was not cut with the tape transfer system and put onto polymer coated slides? If they had done that your tissues would not fall off, not sure of what to do after the fact, but you could certainly try a more gentle antigen retrieval technique such as waterbath (not sure of what you are using as your heat source?) sounds like your retrieval method is too harsh. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph N. Pastore Sent: Saturday, May 08, 2010 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Loss of tissue sections after antigen retrieval Hi All, We have a problem. We are using a commercial tissue array and we find that the two most important tissues are lost during antigen retrieval. The tissues are samples of glioblastomas and are critical to our study. The antigen retrieval system we use is a proprietary 2 step system using a hi and lo pH. Both Ca chelators. Does anyone have suggestions about how we can prevent tissue loss???? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat May 8 18:15:11 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat May 8 18:15:54 2010 Subject: [Histonet] Special stain to demonstrate Silver In-Reply-To: <5476245379016B4D8212E8BCD11ECFFBB77A5C68@AMWPVEX01.kci.com> References: <3c1ed7b7-cb37-40cc-869c-6cb7136c7240@amwpht02.kci.com> <5476245379016B4D8212E8BCD11ECFFBB77A5C68@AMWPVEX01.kci.com> Message-ID: Maria, I am with Terri on this, if there is silver in your tissue it should show up on the H&E as some deposit. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DeGuzman, Maria Sent: Friday, May 07, 2010 6:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special stain to demonstrate Silver Hello Histoneters, Is there a special stain to detect silver in tissue? If there is please share the procedure. Thanks, Fanny Maria V. De Guzman| Histology Technician I Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1482 Email mdeguzman@lifecell.com Mobile 732.688.1386 www.Lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, May 06, 2010 2:16 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. clo test (Tench, Bill) 2. IDEA FOR A NEW RECYCLER!!!! (Madary, Joseph) 3. RE: RE: Barcode and Tracking Information (Rae Staskiewicz) 4. CD 133 (Ingles Claire ) 5. Disposable blade holder - amateur microscopist looking for a cheap one! (Gordon Brown) 6. Disposable blade holder - amateur microscopist looking for a cheap one! (Gordon Brown) 7. NSH Region II Meeting-discount hotel rate deadline is coming up! (Goodwin, Diana) 8. dehydration of hydrated slide.... (Eva Permaul) 9. RE: dehydration of hydrated slide.... (Sebree Linda A) 10. RE: dehydration of hydrated slide.... (Mauger, Joanne) 11. B5 fixative (histotech@imagesbyhopper.com) 12. myocyte damage (Bartlett, Jeanine (CDC/OID/NCZVED)) 13. RE: Responses to IHC CAP Validation question (tonia.richmond@gracepathology.com) 14. Starting up new lab (Shaw, Sharon) 15. Formalin fixation time for breast specimens (Richard Cartun) 16. RE: Responses to IHC CAP Validation question (BSullivan@shorememorial.org) 17. Re: myocyte damage (Merced M Leiker) ---------------------------------------------------------------------- Message: 1 Date: Wed, 5 May 2010 13:57:20 -0700 From: "Tench, Bill" Subject: [Histonet] clo test To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863055@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii The Clo test is a clinical lab test. You need to go to that part of the CPT coding book (sorry I don't have it available). 88300 is an anatomic code (gross only, ie, it requires examination of a piece of tissue or foreign body) and is entirely inappropriate for this test. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, May 05, 2010 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 78, Issue 6 Hi everyone, I am looking to see what CPT code everyone is using for reading Clo Tests in the pathology department. I have heard of using 87081 but I am not sure if this is accurate as this is for culture and the CLO is biochemical reaction not a culture. Currently I have been using 88300 gross only. Any help would be appreciated. Thank you, Amy Farnan *************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 2 Date: Wed, 5 May 2010 17:22:22 -0400 From: "Madary, Joseph" Subject: [Histonet] IDEA FOR A NEW RECYCLER!!!! To: Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1301A37FFA@MD1EV002.medimmune.com> Content-Type: text/plain; charset="us-ascii" Some manufacturer should find a way where you can add all of the chemicals together in one container and when the recycler runs it spits out formalin in one, xylene/hemo-de/f83/ in another and alcohol in another all from one run. To opine on another histnet query from earlier I think both have their place. I use CBG for xylene, form, hemo de and alcohol using the hemo on the processor and depar, alcohol on processor for low grade alc, and xyle for all but the last xylen on the stainer using frsh for that. Creative waste is good and simple but the only thing is mixing old and new formalin can't be good long term. What I was thinking about doing was using creative waste gravimetric for the first run, and then after that use the CBG to redistill NBF for round 2, 3, 4 etc. Hey where am I getting all this money and space? Still like BR recycler too, just do not have one anymore, but liked it. All recycling is good as long as people know which ones to use. Still seems to be an issue for some people. I worked in a place where the techs thought you could throw everyting in one container and the rcycler would spit out clean alcohol, xylene and formalin from one collective run. Hey manufactures? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ------------------------------ Message: 3 Date: Wed, 5 May 2010 17:32:47 -0500 From: "Rae Staskiewicz" Subject: RE: [Histonet] RE: Barcode and Tracking Information To: "'Mahoney,Janice A'" , "'Maggie Allen'" , Cc: histonet-request@lists.utsouthwestern.edu, ":histonet-bounces"@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <926351C712074D27B3AC517297DF0A72@your4105e587b6> Content-Type: text/plain; charset="us-ascii" Jan, Ditto! Ditto! Ditto! Couldn't have said it better myself! Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, May 05, 2010 2:53 PM To: 'Maggie Allen'; histonet@lists.utsouthwestern.edu Cc: histonet-request@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Blake, I think I put in my comments about Ventana's Vantage when you first posted the question, but here goes again.. It is wonderful. Vantage is the best thing to come along in my lifetime as a histo tech! Wonderful for patient safety, easy for the techs to use and a manager's dream for all the data it can provide. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maggie Allen Sent: Wednesday, May 05, 2010 2:24 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Hello Blake, LabelClinic HTS is a tracking system for Histology artifacts within the Pathology workflow. The product is designed to provide improved quality control and traceability with the lab. It provides flexible and reliable labeling and tracking of histology requisitions, containers, specimens, blocks and slides. Using bar code technology, LabelClinic HTS provides an optimized workflow that reduces errors and significantly enhances patient safety. It can be used stand alone, or integrated into an LIS / AP software system. Benefits: * Flexible lab configuration for custom lab workflow * Reduce errors and increase efficiency * Just-in-time reporting of artifact locations or last know location * Alerting for sub-process time violations and missing artifacts * Deploy to Desktop PC's or mobile hand held's * Configurable workflow * Supports color selection for slide and cassette printers I would be happy to set up an online WebEx demo of the system for anyone who may be interested. Thank you! Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel (810) 629-3930 Cell (215) 200-0268 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 5 May 2010 22:15:03 -0500 From: "Ingles Claire " Subject: [Histonet] CD 133 To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Thought I'd try again... Anyone know where I can send some slides for a CD133? Claire ------------------------------ Message: 5 Date: Thu, 6 May 2010 09:16:36 +0100 From: "Gordon Brown" Subject: [Histonet] Disposable blade holder - amateur microscopist looking for a cheap one! To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I dabble in microscopy - well I say I dabble, my wife says otherwise! - and I have a reasonably well equipped home lab, which includes the ubiquitous Cambridge Rocker. I've had reasonably good results with plant tissue sections but sharpening the blades has always proved to be a pain and I tend to get mixed results. However, I've recently acquired a bunch of Accu Edge low profile blades at very low cost but I'm unable to source a holder at an affordable price. IS there anyone out there in professional histology land who has for sale a used, even rough condition holder suitable for these blades? You'd get the grateful thanks of both myself and my wife, who will be more than pleased to see me spend less time muttering about my poor sharpening skills! Many thanks Gordon (UK) ------------------------------ Message: 6 Date: Thu, 6 May 2010 09:36:41 +0100 From: "Gordon Brown" Subject: [Histonet] Disposable blade holder - amateur microscopist looking for a cheap one! To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Perhaps I should point out that despite the date in the UK, my name is genuinely as given, although my namesake may well be looking for a new hobby to fill in his spare time after today........... Gordon ------------------------------ Message: 7 Date: Thu, 6 May 2010 08:18:01 -0400 From: "Goodwin, Diana" Subject: [Histonet] NSH Region II Meeting-discount hotel rate deadline is coming up! To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="windows-1252" You don?t want to miss the upcoming Region II Meeting on June 10-12 in Atlantic City NJ! 25 speakers are presenting 30 different topics from wet workshops to short seminars. CEUs will be granted for all sessions. Over 30 vendors are participating in our Exhibit Area. Your registration fee includes free admission to the Vendor Exhibit, AM and PM Breaks, buffet lunch and Friday evening?s reception. The hotel room rate is reduced to $89/night, but you must reserve by Monday, May 10th! The mail-in registration deadline is May 20th. For more information, you can download a meeting brochure from the NSH website under state meetings at www.nsh.org/content/region-ii-meeting. ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA. ------------------------------ Message: 8 Date: Thu, 06 May 2010 08:56:01 -0400 From: Eva Permaul Subject: [Histonet] dehydration of hydrated slide.... To: histonet@lists.utsouthwestern.edu Message-ID: <4BE2BC61.2040701@georgetown.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University ------------------------------ Message: 9 Date: Thu, 6 May 2010 08:49:06 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] dehydration of hydrated slide.... To: "Eva Permaul" , Message-ID: <8C023B4AB999614BA4791BAEB26E2738399E3B@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="US-ASCII" Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 6 May 2010 11:02:59 -0400 From: "Mauger, Joanne" Subject: RE: [Histonet] dehydration of hydrated slide.... To: Sebree Linda A , Eva Permaul , "histonet@lists.utsouthwestern.edu" Message-ID: <443F5B475A9BF647AB962E834884EBAD2788770DE6@EX7CCRPW03V1.chop.edu> Content-Type: text/plain; charset="us-ascii" Eva, If you dehydrate it again to 100% or xylene, it is less likelt to fall off the slide. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A [LSebree@uwhealth.org] Sent: Thursday, May 06, 2010 9:49 AM To: Eva Permaul; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] dehydration of hydrated slide.... Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 6 May 2010 11:20:01 -0400 From: Subject: [Histonet] B5 fixative To: Message-ID: Content-Type: text/plain; charset="US-ASCII" I was under the impression that B5, because of the mercury content, was outlawed for use Jan 1, 2005. But now I am not so sure! Can anyone tell me if there is a federal law regarding this? We no longer use B5, we use B+, but I know someone in OK who is using B5 (I am in FL). Is he breaking any laws by using it and/or should he be switched to an alternative like B+? Thanks! Michelle ------------------------------ Message: 12 Date: Thu, 6 May 2010 11:16:04 -0400 From: "Bartlett, Jeanine (CDC/OID/NCZVED)" Subject: [Histonet] myocyte damage To: histonet Message-ID: <68510B12184E45498EABD4CB6F3868FE012870BC@LTA3VS001.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii Hello everyone, I am in need of a special stain or an IHC that will demonstrate myocyte damage in muscle tissue...esp. cardiac. Any help will be greatly appreciated. Thanks! Jeanine Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov ------------------------------ Message: 13 Date: Thu, 6 May 2010 10:27:50 -0500 From: tonia.richmond@gracepathology.com Subject: RE: [Histonet] Responses to IHC CAP Validation question To: "McMahon, Loralee A" Cc: "histonet@lists.utsouthwestern.edu" , "thisisann@aol.com" Message-ID: Content-Type: text/plain; charset="UTF-8" If you are a brand new lab, how do you validate IHC if you are no yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer Grace Pathology PH: (501) 765-7367 Email: -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- < To: "thisisann@ "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" Subject: [Histonet] Starting up new lab To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I'm looking at starting up a new histology lab and need everything, I have a tight budget. Can anybody give me recommendations on where to buy refurbished equipment. Thanks Sharon ------------------------------ Message: 15 Date: Thu, 06 May 2010 11:57:43 -0400 From: "Richard Cartun" Subject: [Histonet] Formalin fixation time for breast specimens To: "Histonet" Message-ID: <4BE2AEB7.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII There has been a lot of discussion recently regarding recommendations for formalin fixation of breast specimens. If you are interested in this topic please read the following article published in the May 2010 issue of the American Journal of Clinical Pathology by Ibarra JA, et al., "Fixation time does not affect the expression of estrogen receptor". Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 16 Date: Thu, 6 May 2010 12:16:45 -0400 From: BSullivan@shorememorial.org Subject: RE: [Histonet] Responses to IHC CAP Validation question To: tonia.richmond@gracepathology.com Cc: "histonet@lists.utsouthwestern.edu" , "McMahon, Loralee A" , histonet-bounces@lists.utsouthwestern.edu, "thisisann@aol.com" Message-ID: Content-Type: text/plain; charset=US-ASCII One important thing to remember is that you should make sure All material being used for validation is processed the same way. Will your control tissue be purchased or processed in your lab? Control tissue is used for validation but you should use tissue with various levels of positivity. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 tonia.richmond@gr acepathology.com Sent by: To histonet-bounces@ "McMahon, Loralee A" lists.utsouthwest cc "histonet@lists.utsouthwestern.edu" 05/06/2010 11:27 AM , "thisisann@aol.com" Subject RE: [Histonet] Responses to IHC CAP Validation question If you are a brand new lab, how do you validate IHC if you are no= t yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer = / Laboratory Grace Pathology PH: (501) 765-7367 Email: = [1]tonia.= richmond@gracepathology.com -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- <= /FONT> To: "thisisann@= aol.com" , "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" Sent by: histonet-bounces@lists.u= tsouthwestern.edu Date: 04/28/2010 02:01PM Subject: RE: [Histonet] Re= sponses to IHC CAP Validation question Any inspection that I have under= gone we have used the 25 to 30 case rule. Except for the Er/Pr//Her-2= . We use closer to 50 cases. We also use a TMA to make our live= s easier. The TMA contains known positives and known negatives. I= n cases of t-cell or b-cell markers or cytokeratins. 25 to 30 cases i= s easy. But when you are validated for more hard to find markers (SV-= 40) then fewer cases is acceptable. We always throw in a slide that w= e know will not stain for sv-40 like a tonsil - then you can say it has spe= cificity. Any inspector that I have come across is usually understanding= of this. But I am sure that there are exceptions to this.........esp ecially if they are not familiar with immunohistochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong M= emorial Hospital Department of Surgical Pathology (585) 275-7210 ______________________ 5F__= _______________ From: histonet-bounces@lis= ts.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf= Of thisisann@aol.com [thisisann@aol.com] Sent: Wednesday, April 28, 201= 0 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] R= esponses to IHC CAP Validation question The following is one respone= I rec'd: 1. I asked CAP who told me that they do not currentl= y have a guideline on validating but that they recommend what is in t= he following book: Quality Management In Anatomic Pathology, Promoting P= atient Safety Through Systems Improvement and Error by Raouf E. Nakhl= eh, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8-= Quality Management in IHC That is what we follow. I. Get a new antib= ody and optimize it with your positive control. II. Once optimized you n= eed to run it on cases expected to be positive (how many?) "a suffien= t size ..." III. Must also be run on cases expected to be negative. (how= many? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just = that. (ex. some of the hormones we just use a pituitary) ___________________ ______________________ 5F__= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu = [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________ 5F__ ______________________ Histo= net mailing list Histonet@lists.utsouthwestern.edu [3]http://l= ists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:tonia.richmond@gracepathology.com" 2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 3. 3D"http://=/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 06 May 2010 12:30:50 -0400 From: Merced M Leiker Subject: Re: [Histonet] myocyte damage To: "Bartlett, Jeanine (CDC/OID/NCZVED)" , histonet Message-ID: <5AFA0A6D83365A1F044D4E16@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Stain with cTnI and inspect for damage visually. Just a guess. Regards, Merced --On Thursday, May 06, 2010 11:16 AM -0400 "Bartlett, Jeanine (CDC/OID/NCZVED)" wrote: > Hello everyone, > > I am in need of a special stain or an IHC that will demonstrate myocyte > damage in muscle tissue...esp. cardiac. > > Any help will be greatly appreciated. > > Thanks! > Jeanine Bartlett > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 7 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kristenhinkle <@t> yahoo.com Sun May 9 06:20:01 2010 From: kristenhinkle <@t> yahoo.com (Kristen Reynolds) Date: Sun May 9 06:20:05 2010 Subject: [Histonet] (no subject) Message-ID: <7763.10791.qm@web50008.mail.re2.yahoo.com> http://hahuhilu.t35.com/ From micro <@t> superlink.net Sun May 9 08:19:48 2010 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Sun May 9 08:19:56 2010 Subject: [Histonet] Starting up new lab References: Message-ID: <382495D8AA0143FFA252AEAF9786C474@DJ4VDH31> Please email me for pre-owned histo and EM equipment list. Regards, Markus F. Meyenhofer Microscopy Labs Box 338 Red Bank, NJ 07701 micro@superlink.net ----- Original Message ----- From: "Shaw, Sharon" To: Sent: Thursday, May 06, 2010 11:42 AM Subject: [Histonet] Starting up new lab I'm looking at starting up a new histology lab and need everything, I have a tight budget. Can anybody give me recommendations on where to buy refurbished equipment. Thanks Sharon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atouvr <@t> yahoo.com Sun May 9 11:06:19 2010 From: atouvr <@t> yahoo.com (annamaria touvra) Date: Sun May 9 11:06:22 2010 Subject: [Histonet] Re: Histonet Digest, Vol 78, Issue 10 Message-ID: <152735.38354.qm@web65615.mail.ac4.yahoo.com> Hello! Is there anybody?who could?discuss?some aorta Von Kossa's staining??? I could send some representative images with some questiones to be discussed. Thank you in advance, Anna-Maria ? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Anna-Maria Touvra PhD Candidate Democritus University of Thrace Department of Physical Education and Sport Science Address: University Campus,?TEFAA Komotini 69100, GREECE Tel: +306938511677 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From louise.renton <@t> gmail.com Mon May 10 03:19:56 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Mon May 10 03:20:00 2010 Subject: [Histonet] Cryostat vs decal sections of bone Message-ID: Hi all, last year sometime I was asked to budget for some equipment for our unit. Not expecting to get anything i aimed high, and requested a cryostat and cryoJane system. Lo and behold, the planetary influences were just right, and my request was approved. Now, I've got cold feet (pardon the pun) and I'm wondering if there *are* any distinct advantages of cryosections of bone over demineralised wax embedded samples in regards to immuno and in-situ ?. -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Heather.Mcleod <@t> uct.ac.za Mon May 10 06:26:20 2010 From: Heather.Mcleod <@t> uct.ac.za (Heather McCleod) Date: Mon May 10 06:26:39 2010 Subject: [Histonet] IgG4 source In-Reply-To: References: Message-ID: <4BE8097C020000CB0003516F@gwiasmtp.uct.ac.za> I have used invitrogen - mouse anti-human IgG4 Clone HP6025 (successfully) AR in 1mm EDTA Dilution 1:400 Control - tonsil (or Sjogren's Syndrome) >>> Joao Pessoa 2010/05/07 11:50 PM >>> I need to find a source for an IgG4 antibody for use with IHC studies on human FFPE tissue. Any ideas? My preference would be for a concentrated IVD. Thanks, Joao Histo Tech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________________________________ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 4500. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. _____________________________________________________________________________________________________ From JKELL2 <@t> capefearvalley.com Mon May 10 07:43:30 2010 From: JKELL2 <@t> capefearvalley.com (Jason Keller) Date: Mon May 10 07:43:47 2010 Subject: [Histonet] Number of Techs Message-ID: <7D41E97C787DF44996E0055C81703663F17308@ntexchange3.capefear.local> Hello, I am looking to compile information on the average number of techs that different hospitals have in their histology labs. I would appreciate any feedback that I could get as far as how many techs are used to run hospital histology labs with a block count range of 200 to 400 blocks per day. Thanks for any input you can provide, Jason CONFIDENTIALITY NOTICE: This electronic mail transmission may contain information that is privileged and/or confidential. Additionally, this communication may contain individual protected health information ("PHI") that is subject to protection under state and federal laws, or other privileged, confidential or proprietary information of Cape Fear Valley Health System that may not be further disclosed. Please be advised that any disclosure, copying, distribution or other use of the contents of this message by anyone other than the intended recipient is prohibited. If you have received this communication in error, please notify the sender immediately by replying to the message and deleting it from your computer. From rjbuesa <@t> yahoo.com Mon May 10 07:51:03 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 10 07:51:16 2010 Subject: [Histonet] Cryostat vs decal sections of bone In-Reply-To: Message-ID: <868067.13979.qm@web65711.mail.ac4.yahoo.com> Most definitely there are differences. Just consider that the bone will not be subjected to any chemicals because neither fixation nor demineralization will be required., Ren? J. --- On Mon, 5/10/10, louise renton wrote: From: louise renton Subject: [Histonet] Cryostat vs decal sections of bone To: Histonet@lists.utsouthwestern.edu Date: Monday, May 10, 2010, 4:19 AM Hi all, last year sometime I was asked to budget for some equipment for our unit. Not expecting to get anything i aimed high, and requested a cryostat and cryoJane system. Lo and behold, the planetary influences were just right, and my request was approved. Now, I've got cold feet (pardon the pun) and I'm wondering if there *are*? any distinct advantages of cryosections of bone over demineralised wax embedded samples in regards to immuno and in-situ ?. -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Mon May 10 12:04:02 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon May 10 12:04:20 2010 Subject: [Histonet] Re: Bone Frozen sections versus decalcified bone in paraffin Message-ID: <000301caf062$cb4a35e0$61dea1a0$@callis@bresnan.net> Louise, You wrote: last year sometime I was asked to budget for some equipment for our unit.Not expecting to get anything i aimed high, and requested a cryostat and cryoJane system. Lo and behold, the planetary influences were just right, and my request was approved. Now, I've got cold feet (pardon the pun) and I'm wondering if there *are* any distinct advantages of cryosections of bone over demineralised wax embedded samples in regards to immuno and in-situ ?. Yes there are distinct advantages for using Cryojane. Undecalcifed bone frozen sections are done in our lab in order to do immunostaining, either enzyme immunohistochemistry or immunofluorescence work on fresh undecalcifed bone frozen sections, especially when murine CD markers and GFP are compromised by aldehyde fixatives (PFA or NBF), acid decalcification, or aldehyde induced autofluorescence when working with GFP . There are ways to get around the autofluorescence problem. We use Cryojane for all undecalcifed bone frozen sections, but also section other very difficult frozen tissues. There are times using FFPE/decalcified bone is not going to stain successfully for either immuno or ISH methods IF the antigens are compromised by either mentioned methods, plus heat from paraffin processing. Cryojane is our backup method when things go awry, and our method of choice with most GFP work with murine nasal turbinates. Trying to do "free hand" frozen sections on undecalcified bone was an unsuccessful nightmare, very time consuming with terrible results (crunched up bone sections). Our problem has been too many murine CD markers we work with only stain on fresh frozen sections after solvent fixation. If most of your antigens (or ISH) stain successfully with decalcified, FFPE sections, then you may not need Cryojane but that is something you have to work out. We find Cryojane a necessity for our work. One can decalcify bone prior to fixation Mori et al then cut frozen sections, fix and stain - a long drawn out procedure for IHC. There are others who have success on undecalcifed bone frozen sections ( Kusser et al with excellent references) Both of these publications are now freely accessible in J Histochem Cytochem. ISH is more difficult on acid decalcified bone, where EDTA may have to be used for FFPE. There are some excellent publications on using decalcified bone (marrow) with ISH, the most recent from Gudrun Lang in Journal of Histotechnology, March 2010 describing kappa and lambda ISH on acid bone marrow biopsies. She listed several excellent references on ISH/IHC for FFPE bone samples. Gudrun participates on Histonet and should be able to drop a pdf of this publication to you. I have also done PLP fixed, EDTA decalcified bone, sucrose cryoprotected bone frozen sections mounted on Plus Charge slides, but prefixed bone frozen sections often release from the slide surface during staining. That is the joy of Cryojane, no release. Working with the instrument takes some practice, and there is an excellent publication by John Tarpley in J of Histotechnology for doing this. Cryojane is unique but be sure it fits in your cryostat before purchase. There is the Kawamoto tape method for bone frozen sections, but it requires purchasing a knife holder, and I believe, special knives. The section is NOT transferred to a slide, but remains on the tape where all staining takes place, then mount the tape onto a slide in a special way. Those who use it acquire beautiful staining results for both IHC and ISH. This method has been around for awhile and you have to purchase all the accessories and tapes from a company in Japan. I know that Jamie Erickson is using this and has also worked with Cryojane. Hopefully Jamie is looking in and can comment. Good luck, Gayle M. Callis HTL/HT/MT(ASCP) From tanisha.neely <@t> covance.com Mon May 10 12:38:48 2010 From: tanisha.neely <@t> covance.com (Neely, Tanisha) Date: Mon May 10 12:39:07 2010 Subject: [Histonet] Protocol Needed for Processing Previously Snap Frozen Tissue Message-ID: <816E3C72F855F14985FC31D7C963AE6F1D78F6A2@indexch03.ent.covance.com> Hello Histo-Netters: Does anyone have a protocol for post processing (FFPE) frozen tissue samples? Tanisha Neely HT(ASCP) ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From kjones <@t> cellmarque.com Mon May 10 12:47:32 2010 From: kjones <@t> cellmarque.com (Kelsey Jones) Date: Mon May 10 12:47:40 2010 Subject: SPAM-LOW: [Histonet] Loss of tissue sections after antigenretrieval In-Reply-To: <74FC819FCC4D439B96D37E23642D67C9@prueggihctechlt> References: <74FC819FCC4D439B96D37E23642D67C9@prueggihctechlt> Message-ID: <7F2A2AE306CE254DB7279E86A51A7406012E8CD4@CMROCEX01.cellmarque.local> Patsy is correct, there is a very good chance the retrieval is the issue. Another possible issue is the drying time. We find often times with tissue loss we can remedy that with extended drying times. If we get precut slides from an outside source, we dry them for 2 hours in a 58 degree oven to be cautious. Kelsey Jones Technical Consultant 6600 Sierra College Blvd. Rocklin, CA 95677 (916) 746-8900 x8969 (916) 746-8989 (fax) www.cellmarque.com **Check out Cell Marque on Facebook! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Saturday, May 08, 2010 4:11 PM To: 'Joseph N. Pastore'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] Loss of tissue sections after antigenretrieval Joe, Did you buy an array that was not cut with the tape transfer system and put onto polymer coated slides? If they had done that your tissues would not fall off, not sure of what to do after the fact, but you could certainly try a more gentle antigen retrieval technique such as waterbath (not sure of what you are using as your heat source?) sounds like your retrieval method is too harsh. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph N. Pastore Sent: Saturday, May 08, 2010 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Loss of tissue sections after antigen retrieval Hi All, We have a problem. We are using a commercial tissue array and we find that the two most important tissues are lost during antigen retrieval. The tissues are samples of glioblastomas and are critical to our study. The antigen retrieval system we use is a proprietary 2 step system using a hi and lo pH. Both Ca chelators. Does anyone have suggestions about how we can prevent tissue loss???? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Mon May 10 13:06:22 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon May 10 13:06:31 2010 Subject: [Histonet] Re: Cryostat vs decal sections of bone Message-ID: Hi Louise, Remember me? It's been a while. You asked if there were distinct advantages to using cryosections of bone over decalcified wax samples for IHC and ISH and the answer is yes, of course. Some antibodies and probes just won't work well in paraffin processed samples, either due to the decalcification process or the alcohol/solvent exposure. The ISH purists know that just paraffin processing can diminish signal greatly. Having said that, obtaining excellent sections of undecalcified, fresh frozen bone is tricky. Very tricky indeed, and what works one day doesn't work the next. You'll need to have at least one tungsten carbide knife for it, so make sure you also get the regular blade holder. I don't know if you still use steel knives on the cryostat, and if so you'll already have the correct blade holder, but we use disposables so we needed to order a separate one. We've had pretty good luck getting beautiful sections of fixed and decalcified cryosections and the cryojane, and this would work well for samples that just flat don't like paraffin processing but the antibody staining does ok with formalin and formic acid treatment. Having the system opens up this possibility for your studies as well. Best wishes always, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From rshooki_99 <@t> yahoo.com Mon May 10 13:23:00 2010 From: rshooki_99 <@t> yahoo.com (richard shook) Date: Mon May 10 13:23:11 2010 Subject: [Histonet] per deim work Message-ID: <629474.84559.qm@web36103.mail.mud.yahoo.com> Hello Histoland I have question pertaining to temporary histology tech work/per Diem positions.I find myself currently out of work and am looking for my next adventure in? the great world of histology and one of the ideas i had was to check out the possibility's of doing temp work.The only thing is I'm not sure if there is a temp agency for histo techs,or is there someone in histo land that currently working as a temp . I would greatly appreciate any help in my endeavors into this new world. Any information as to how much the pay rate or what?to charge for daily work travel?lodging or any other expenses that would be incurred in this?type of work. Thanks for any help Richard Shook HT(ASCP) From mwhite <@t> mcleodhealth.org Mon May 10 13:35:09 2010 From: mwhite <@t> mcleodhealth.org (mwhite@mcleodhealth.org) Date: Mon May 10 13:35:19 2010 Subject: [Histonet] Specimen Delivery Message-ID: For those of you who do tissue/cytology processing in a hospital or similar facility: Are tissue or fluid specimens brought directly to the Anatomic Pathology/Cytology area, or are specimens dropped off in a clinical accessioning area ? We are debating the pros/cons of having our specimens dropped at a centralized location since our Lab is becoming more "spread out" and nurses are confused about where to bring stuff. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 From ritchiej <@t> upstate.edu Mon May 10 14:08:50 2010 From: ritchiej <@t> upstate.edu (Julie Ritchie) Date: Mon May 10 14:09:00 2010 Subject: [Histonet] Curling floating mouse brains Message-ID: <4BE821830200002C0006023C@gwmta2.upstate.edu> Hello everyone. I am slicing mouse brains that were perfused with 4% paraformaldehdye in PBS, then sunk in 30% sucrose in PBS. I want to cut the brains in 40 um sections, collect in wells of PBS, and do IHC. BUT my sections keep curling into little cannolis (or even tighter, like those pirouette cookies). Just a few weeks ago I sectioned 24 rat brains in this manner without any problem at all. I did the perfusions for all of these animals, so it is not an issue of inadequate perfusion. I asked someone if it could be related to size of tissue (mouse vs. rat), and it was suggested that 30% scucrose was too high for mouse brains, so I melted them out of frozen OCT (kept in a frig overnight in PBS), resunk brains in 20% sucrose/PBS and refroze in OCT again. They did indeed slice better at this conc of sucrose (no streaks, shredding, etc. when slicing) but the brains sure did curl still in the PBS!!! I thought the sections were too thick, so dropped to 30 um, and still there is curling. Even 20 um sections curl up in the PBS. It is not a humidity problem, or a blade problem, for I have tried to cut on another machine, and tissue still curled. Cutting temp is 13-14 degrees, same as used for rat brains. Tried different PBS---> same curling. I am at a loss. Any advice would be very helpful. Thank you. From lpaveli1 <@t> hurleymc.com Mon May 10 14:13:02 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon May 10 14:13:25 2010 Subject: [Histonet] Bone marrow smear staining In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F1D78F6A2@indexch03.ent.covance.com> References: <816E3C72F855F14985FC31D7C963AE6F1D78F6A2@indexch03.ent.covance.com> Message-ID: <4BE8227E.59CD.00EE.0@hurleymc.com> Need Histonet help. Hematology recently purchased a new blood smear stainer that is producing variable staining. It is especially bad with the bone marrow smears, even when it is run through twice as was the norm with the old stainer (now gone). Until this is resolved, does anyone have a GOOD MANUAL wright-giemsa staining protocol you would like to share? Our pathologists are quite frustrated. They do not care for the May-Grunwald Giemsa that we do manually for our hematology oncologists. thank you for your quick responses!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 From laurie.colbert <@t> huntingtonhospital.com Mon May 10 14:23:09 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon May 10 14:23:22 2010 Subject: [Histonet] Specimen Delivery Message-ID: <57BE698966D5C54EAE8612E8941D768308BCA679@EXCHANGE3.huntingtonhospital.com> Tissue specimens are brought directly to pathology. Cytology fluids are dropped off in Processing, because often fluids need to be shared between several departments, and our Cytology dept doesn't necessarily know that it is a shared specimen. Processing can look it up in the LIS. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mwhite@mcleodhealth.org Sent: Monday, May 10, 2010 11:35 AM To: histonet@lists.utsouthwestern.edu Cc: TBailey@mcleodhealth.org Subject: [Histonet] Specimen Delivery For those of you who do tissue/cytology processing in a hospital or similar facility: Are tissue or fluid specimens brought directly to the Anatomic Pathology/Cytology area, or are specimens dropped off in a clinical accessioning area ? We are debating the pros/cons of having our specimens dropped at a centralized location since our Lab is becoming more "spread out" and nurses are confused about where to bring stuff. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Mon May 10 14:25:49 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon May 10 14:26:17 2010 Subject: [Histonet] Bone marrow wright stain In-Reply-To: <629474.84559.qm@web36103.mail.mud.yahoo.com> References: <629474.84559.qm@web36103.mail.mud.yahoo.com> Message-ID: <4BE8257D.59CD.00EE.0@hurleymc.com> Hello all, Need Histonet help. Hematology recently purchased a new blood smear stainer that is producing variable staining. It is especially bad with the bone marrow smears, even when it is run through twice as was the norm with the old stainer (now gone). Until this is resolved, does anyone have a GOOD MANUAL wright-giemsa staining protocol you would like to share? Our pathologists are quite frustrated. They do not care for the May-Grunwald Giemsa that we do manually for our hematology oncologists. thank you for your quick responses!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 From srodriguez <@t> phenopath.com Mon May 10 14:45:11 2010 From: srodriguez <@t> phenopath.com (Stephanie Rodriguez) Date: Mon May 10 14:45:32 2010 Subject: [Histonet] Re: Histonet Digest, Vol 78, Issue 12 Message-ID: We use this clone also. It is distributed by Invitrogen, but the company that makes it is CalTag. (Maybe Invitrogen owns them.) We use AR of ph 9.0 Tris (0.5mM)/EDTA (0.1mM) for 20 min, dilution of 1:2000, and detection of Dako Flex+. It is not an IVD, by the way; it?s still RUO. Stephanie Rodriguez, HTL(ASCP), QIHC Senior Molecular Tech/IHC Tech III Phenopath Laboratories Seattle, WA On 5/10/10 10:43 AM, "histonet-request@lists.utsouthwestern.edu" wrote: > Message: 2 > Date: Mon, 10 May 2010 13:26:20 +0200 > From: "Heather McCleod" > Subject: Re: [Histonet] IgG4 source > To: "Joao Pessoa" , > > Message-ID: <4BE8097C020000CB0003516F@gwiasmtp.uct.ac.za> > Content-Type: text/plain; charset=US-ASCII > > I have used invitrogen - mouse anti-human IgG4 Clone HP6025 (successfully) > AR in 1mm EDTA Dilution 1:400 > Control - tonsil (or Sjogren's Syndrome) > >>>> >>> Joao Pessoa 2010/05/07 11:50 PM >>> > I need to find a source for an IgG4 antibody for use with IHC studies on > human FFPE tissue. Any ideas? My preference would be for a > concentrated IVD. > > Thanks, > > Joao > Histo Tech > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From cbarone <@t> NEMOURS.ORG Mon May 10 15:25:10 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon May 10 15:25:16 2010 Subject: [Histonet] Region II Managers Forum Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7CD8@wlmmsx01.nemours.org> Region II Managers on the Histonet: Opportunity to attend the Managers Forum - Region II Meeting/Atlantic City West, NJ. Date: June 10 Time: 1:30-5:00PM Session Title: "Manager's Forum: Roundtable Discussion for Anatomic Pathology Managers and Core Labs" Panel of Experts: Carol Barone Director Histotech Core Lab, Nemours - A.I. duPont Hospital for Children, Wilmington, DE Janice Alvarez Anatomic Pathology Project Manager, Johns Hopkins Hospital, Baltimore, MD Melissa Gannon Histology Manager, DIANON/Laboratory Corporation of America, Raleigh, NC Moderator: Diana Goodwin, Previous Anatomic Pathology Supervisor Pennsylvania Hospital in Phila,PA This informal discussion among peers, will be conducted by and for individuals who are in a supervisory or managerial roles encompassing technical and/or administrative oversight in histotechnology. Registrants are encouraged to submit topics of interest, or concern via e-mail to :dgoodwin100@comcast.net at least two weeks prior to the event. Participants will earn 3 CEU for attending this half day workshop. Cost of this session is $40 for state society or Region II members or $60 for non-members. Deadline is May 20th To download a meeting brochure and registration information, go to the Region II meeting announcement under the state meeting information on the NSH website at www.nsh.org/content/region-ii-meeting. From tpodawiltz <@t> lrgh.org Mon May 10 15:54:54 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon May 10 15:56:59 2010 Subject: [Histonet] Specimen Delivery In-Reply-To: References: Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5B1FB0E@LRGHEXVS1.practice.lrgh.org> Here, all the surgical specimens are delivered directly to the Histology lab. All other specimens are delivered to the Specimen processing area (SPA). This way all specimens that need to be shared can be done there. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mwhite@mcleodhealth.org [mwhite@mcleodhealth.org] Sent: Monday, May 10, 2010 2:35 PM To: histonet@lists.utsouthwestern.edu Cc: TBailey@mcleodhealth.org Subject: [Histonet] Specimen Delivery For those of you who do tissue/cytology processing in a hospital or similar facility: Are tissue or fluid specimens brought directly to the Anatomic Pathology/Cytology area, or are specimens dropped off in a clinical accessioning area ? We are debating the pros/cons of having our specimens dropped at a centralized location since our Lab is becoming more "spread out" and nurses are confused about where to bring stuff. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Sharon.Davis-Devine <@t> carle.com Mon May 10 16:08:51 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Mon May 10 16:09:06 2010 Subject: [Histonet] Histology Labs work schedules Message-ID: I have an important question to pose to all of you Histonetters. We currently have 5 Histotechs who work from 4:00 am to 4:30 pm Monday thru Friday. One comes in at 4 am to embed, another comes in at 5 am to help finish embedding and start cutting blocks. The others come in from around 7 am to 8 am. We average around 200-250 blocks per day with an average of 350-400 slides cut per day. IHC and Special staining is averaging around 145-200 slides per day with that number increasing. So the burden of the work rests on these 5 techs in the morning. We have a tech from Cytology who performs all of the IHC staining using two Ventana Benchmark stainers. The problem is our IHC volume keeps increasing so that two runs a day are not keeping up with the volume. We are playing around with the idea of adding an additional shift so that we have personnel here later in the day to run the IHC and special stainers and to embed and cut small biopsies and breast specimens. Below is a list of questions that I would like for you to answer to assist us in making these crucial decisions. 1. What are your staff schedules? HT's, PA's and Lab assistants. 2. On average, how many blocks and slides do you cut per day? 3. If you have more than one shift, what hours do they work and what job functions do they perform? 4. Does any of your staff work on the weekend and if they do what is their job function? 5. What kind of QC/QA program do you utilize in your laboratory? Thank you so much for you help. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From tjay30 <@t> yahoo.com Mon May 10 19:24:30 2010 From: tjay30 <@t> yahoo.com (Timothy Jay) Date: Mon May 10 19:24:34 2010 Subject: [Histonet] P/T Certified Techs Needed (Multiple Openings) Message-ID: <238.78862.qm@web34306.mail.mud.yahoo.com> I have job openings in Santa Rosa, Folsom, and Torrance, California for part-time certified histotechs. Great opportunity to work in brand new, well furnished, high quality, ?in-office path labs with great physicians. Competitive pay and flexible hours. Send resumes to Timothy Garcia-Jay at tjay30@yahoo.com ? Thank you. ? Tim From sandoval.1965 <@t> hotmail.com Mon May 10 23:00:38 2010 From: sandoval.1965 <@t> hotmail.com (Anthony Sandoval) Date: Mon May 10 22:58:26 2010 Subject: [Histonet] Are Histotechs considered "exempt" employees? Message-ID: Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! From lpwenk <@t> sbcglobal.net Tue May 11 05:31:22 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue May 11 05:31:38 2010 Subject: [Histonet] Are Histotechs considered "exempt" employees? In-Reply-To: Message-ID: <5679D876538D4E2C995F996B8AD114CC@HPPav2> Hospitals/labs can make any position exempt or non-exempt. That's up to them. However, I'd say you are being underpaid. According to the last ASCP Wage/Salary survey (completed 2008, published 2009), the median range for HTL was about $20 - $25 per hour, with the median average being $23.46/hour. (ASCP is getting ready to do another survey in 2010, to be published in 2011.) This is what the vacancy rate was in the 2008 survey: "Histotechnicians (HT)/Histotechnologists (HTL): The vacancy rate for HTs was 8.0%, among the highest vacancy rates reported across all of the surveyed positions. Laboratories in the Far West and laboratories that performed medium-volume testing reported very high vacancy rates, 21.0% and 33.8%, respectively. Private clinic/reference laboratories and hospitals with 500+ beds had vacancy rates of 12.2% and 12.1%, respectively. The vacancy rate for HT supervisors was 4.1%. Although subsample sizes for some demographic groups were too small for meaningful statistical analysis, vacancy rates for HT Supervisors were notably higher among hospitals with 500+ beds (8.5%) and laboratories in the East North Central region (24.7%). For HTLs, the vacancy rate was 7.2%. Laboratories in the Far West reported a vacancy rate of 9.5%." California is "Far West" in the survey. http://www.ascp.org/pdf/Membership-Communications/Wage-and-Vacancy-Survey.as px Can you do a survey of other histology labs in your area of wage and vacancy rates? Or have your Human Resources department do it? Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Sandoval Sent: Tuesday, May 11, 2010 12:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Histotechs considered "exempt" employees? Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Tue May 11 06:02:33 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue May 11 06:02:48 2010 Subject: [Histonet] need tips for cross-sectioning of cortical bone In-Reply-To: <63EA0607835FBA4689CEA9EA8B48269202FFDF8D@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B48269202FFDF8D@usctmx1141.merck.com> Message-ID: Why not embed in resin (MMA) and take thicker sections and then grind/ polish them down? If you went this route, you could then use flourescent labels and quantify mineral apposition rate and bone formation rate. Let me know if you are interested. I can help you get started and direct you to low cost equipment options. Jack On Apr 22, 2010, at 9:58 AM, "Connolly, Brett M" wrote: > A colleague is having trouble getting wrinkle-free sections of > decalcified, paraffin embedded femur. > > Any tips?? > > Thanks, > > Brett M. Connolly, Ph.D. > Molecular Imaging Team Leader > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > tel. 215-652-2501 fax. 215-993-6803 > brett_connolly@merck.com > > > > Notice: This e-mail message, together with any attachments, > contains information of Merck & Co., Inc. (One Merck Drive, > Whitehouse Station, New Jersey, USA 08889), and/or its affiliates > Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html > ) that may be confidential, proprietary copyrighted and/or legally > privileged. It is intended solely for the use of the individual or > entity named on this message. If you are not the intended recipient, > and have received this message in error, please notify us > immediately by reply e-mail and then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tjasper <@t> copc.net Tue May 11 06:11:38 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue May 11 06:11:44 2010 Subject: FW: [Histonet] Are Histotechs considered "exempt" employees? Message-ID: <90354A475B420441B2A0396E5008D49695E907@copc-sbs.COPC.local> -----Original Message----- From: Thomas Jasper Sent: Tuesday, May 11, 2010 3:45 AM To: 'Anthony Sandoval' Cc: 'histonet-bounces@lists.utsouthwestern.edu' Subject: RE: [Histonet] Are Histotechs considered "exempt" employees? Anthony, Don't know where you live in Cali or where you work. But if you are an HTL and have any decent skill/experience, I would think you are being totally ripped off. The cost of living in most parts of Cali alone makes me wonder about this salary. And when you ask about being exempt...I'm assuming you are exempt? If this is the case again a total rip off. I think you need to look for work elsewhere and check into wages. New students that are registry eligible start out much higher than that to my knowledge. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor CORPS Bend, Oregon -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Sandoval Sent: Monday, May 10, 2010 9:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Histotechs considered "exempt" employees? Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From baustin <@t> cbgbiotech.com Tue May 11 06:43:14 2010 From: baustin <@t> cbgbiotech.com (Beth Austin) Date: Tue May 11 06:43:31 2010 Subject: [Histonet] RE: Idea for a new recycler Message-ID: If formalin, xylene, hemo-de, Formula 83 and alcohol were all collected as one waste stream, no recycler could separate this waste into recycled formalin, recycled solvent (xylene/hemo-de/F83), and recycled alcohol all in one run due in part to the small range of boiling points and in part to the azeotropes formed between the various chemicals. In this particular scenario, the alcohol and the formalin would distill over together along with any water in the formalin, and there would be a considerable amount solvent in the alcohol making it contaminated and not of any use in the lab. If you have any questions, please contact us off site and we'll be happy to assist you. Best Regards, Elizabeth Sell CBG Biotech 1-800-941-9484 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. clo test (Tench, Bill) 2. IDEA FOR A NEW RECYCLER!!!! (Madary, Joseph) 3. RE: RE: Barcode and Tracking Information (Rae Staskiewicz) 4. CD 133 (Ingles Claire ) 5. Disposable blade holder - amateur microscopist looking for a cheap one! (Gordon Brown) 6. Disposable blade holder - amateur microscopist looking for a cheap one! (Gordon Brown) 7. NSH Region II Meeting-discount hotel rate deadline is coming up! (Goodwin, Diana) 8. dehydration of hydrated slide.... (Eva Permaul) 9. RE: dehydration of hydrated slide.... (Sebree Linda A) 10. RE: dehydration of hydrated slide.... (Mauger, Joanne) 11. B5 fixative (histotech@imagesbyhopper.com) 12. myocyte damage (Bartlett, Jeanine (CDC/OID/NCZVED)) 13. RE: Responses to IHC CAP Validation question (tonia.richmond@gracepathology.com) 14. Starting up new lab (Shaw, Sharon) 15. Formalin fixation time for breast specimens (Richard Cartun) 16. RE: Responses to IHC CAP Validation question (BSullivan@shorememorial.org) 17. Re: myocyte damage (Merced M Leiker) ---------------------------------------------------------------------- Message: 1 Date: Wed, 5 May 2010 13:57:20 -0700 From: "Tench, Bill" Subject: [Histonet] clo test To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863055@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii The Clo test is a clinical lab test. You need to go to that part of the CPT coding book (sorry I don't have it available). 88300 is an anatomic code (gross only, ie, it requires examination of a piece of tissue or foreign body) and is entirely inappropriate for this test. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, May 05, 2010 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 78, Issue 6 Hi everyone, I am looking to see what CPT code everyone is using for reading Clo Tests in the pathology department. I have heard of using 87081 but I am not sure if this is accurate as this is for culture and the CLO is biochemical reaction not a culture. Currently I have been using 88300 gross only. Any help would be appreciated. Thank you, Amy Farnan *************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 2 Date: Wed, 5 May 2010 17:22:22 -0400 From: "Madary, Joseph" Subject: [Histonet] IDEA FOR A NEW RECYCLER!!!! To: Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1301A37FFA@MD1EV002.medimmune.com> Content-Type: text/plain; charset="us-ascii" Some manufacturer should find a way where you can add all of the chemicals together in one container and when the recycler runs it spits out formalin in one, xylene/hemo-de/f83/ in another and alcohol in another all from one run. To opine on another histnet query from earlier I think both have their place. I use CBG for xylene, form, hemo de and alcohol using the hemo on the processor and depar, alcohol on processor for low grade alc, and xyle for all but the last xylen on the stainer using frsh for that. Creative waste is good and simple but the only thing is mixing old and new formalin can't be good long term. What I was thinking about doing was using creative waste gravimetric for the first run, and then after that use the CBG to redistill NBF for round 2, 3, 4 etc. Hey where am I getting all this money and space? Still like BR recycler too, just do not have one anymore, but liked it. All recycling is good as long as people know which ones to use. Still seems to be an issue for some people. I worked in a place where the techs thought you could throw everyting in one container and the rcycler would spit out clean alcohol, xylene and formalin from one collective run. Hey manufactures? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ------------------------------ Message: 3 Date: Wed, 5 May 2010 17:32:47 -0500 From: "Rae Staskiewicz" Subject: RE: [Histonet] RE: Barcode and Tracking Information To: "'Mahoney,Janice A'" , "'Maggie Allen'" , Cc: histonet-request@lists.utsouthwestern.edu, ":histonet-bounces"@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <926351C712074D27B3AC517297DF0A72@your4105e587b6> Content-Type: text/plain; charset="us-ascii" Jan, Ditto! Ditto! Ditto! Couldn't have said it better myself! Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, May 05, 2010 2:53 PM To: 'Maggie Allen'; histonet@lists.utsouthwestern.edu Cc: histonet-request@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Blake, I think I put in my comments about Ventana's Vantage when you first posted the question, but here goes again.. It is wonderful. Vantage is the best thing to come along in my lifetime as a histo tech! Wonderful for patient safety, easy for the techs to use and a manager's dream for all the data it can provide. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maggie Allen Sent: Wednesday, May 05, 2010 2:24 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Hello Blake, LabelClinic HTS is a tracking system for Histology artifacts within the Pathology workflow. The product is designed to provide improved quality control and traceability with the lab. It provides flexible and reliable labeling and tracking of histology requisitions, containers, specimens, blocks and slides. Using bar code technology, LabelClinic HTS provides an optimized workflow that reduces errors and significantly enhances patient safety. It can be used stand alone, or integrated into an LIS / AP software system. Benefits: * Flexible lab configuration for custom lab workflow * Reduce errors and increase efficiency * Just-in-time reporting of artifact locations or last know location * Alerting for sub-process time violations and missing artifacts * Deploy to Desktop PC's or mobile hand held's * Configurable workflow * Supports color selection for slide and cassette printers I would be happy to set up an online WebEx demo of the system for anyone who may be interested. Thank you! Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel (810) 629-3930 Cell (215) 200-0268 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 5 May 2010 22:15:03 -0500 From: "Ingles Claire " Subject: [Histonet] CD 133 To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Thought I'd try again... Anyone know where I can send some slides for a CD133? Claire ------------------------------ Message: 5 Date: Thu, 6 May 2010 09:16:36 +0100 From: "Gordon Brown" Subject: [Histonet] Disposable blade holder - amateur microscopist looking for a cheap one! To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I dabble in microscopy - well I say I dabble, my wife says otherwise! - and I have a reasonably well equipped home lab, which includes the ubiquitous Cambridge Rocker. I've had reasonably good results with plant tissue sections but sharpening the blades has always proved to be a pain and I tend to get mixed results. However, I've recently acquired a bunch of Accu Edge low profile blades at very low cost but I'm unable to source a holder at an affordable price. IS there anyone out there in professional histology land who has for sale a used, even rough condition holder suitable for these blades? You'd get the grateful thanks of both myself and my wife, who will be more than pleased to see me spend less time muttering about my poor sharpening skills! Many thanks Gordon (UK) ------------------------------ Message: 6 Date: Thu, 6 May 2010 09:36:41 +0100 From: "Gordon Brown" Subject: [Histonet] Disposable blade holder - amateur microscopist looking for a cheap one! To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Perhaps I should point out that despite the date in the UK, my name is genuinely as given, although my namesake may well be looking for a new hobby to fill in his spare time after today........... Gordon ------------------------------ Message: 7 Date: Thu, 6 May 2010 08:18:01 -0400 From: "Goodwin, Diana" Subject: [Histonet] NSH Region II Meeting-discount hotel rate deadline is coming up! To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="windows-1252" You dont want to miss the upcoming Region II Meeting on June 10-12 in Atlantic City NJ! 25 speakers are presenting 30 different topics from wet workshops to short seminars. CEUs will be granted for all sessions. Over 30 vendors are participating in our Exhibit Area. Your registration fee includes free admission to the Vendor Exhibit, AM and PM Breaks, buffet lunch and Friday evenings reception. The hotel room rate is reduced to $89/night, but you must reserve by Monday, May 10th! The mail-in registration deadline is May 20th. For more information, you can download a meeting brochure from the NSH website under state meetings at www.nsh.org/content/region-ii-meeting. ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA. ------------------------------ Message: 8 Date: Thu, 06 May 2010 08:56:01 -0400 From: Eva Permaul Subject: [Histonet] dehydration of hydrated slide.... To: histonet@lists.utsouthwestern.edu Message-ID: <4BE2BC61.2040701@georgetown.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University ------------------------------ Message: 9 Date: Thu, 6 May 2010 08:49:06 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] dehydration of hydrated slide.... To: "Eva Permaul" , Message-ID: <8C023B4AB999614BA4791BAEB26E2738399E3B@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="US-ASCII" Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 6 May 2010 11:02:59 -0400 From: "Mauger, Joanne" Subject: RE: [Histonet] dehydration of hydrated slide.... To: Sebree Linda A , Eva Permaul , "histonet@lists.utsouthwestern.edu" Message-ID: <443F5B475A9BF647AB962E834884EBAD2788770DE6@EX7CCRPW03V1.chop.edu> Content-Type: text/plain; charset="us-ascii" Eva, If you dehydrate it again to 100% or xylene, it is less likelt to fall off the slide. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A [LSebree@uwhealth.org] Sent: Thursday, May 06, 2010 9:49 AM To: Eva Permaul; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] dehydration of hydrated slide.... Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 6 May 2010 11:20:01 -0400 From: Subject: [Histonet] B5 fixative To: Message-ID: Content-Type: text/plain; charset="US-ASCII" I was under the impression that B5, because of the mercury content, was outlawed for use Jan 1, 2005. But now I am not so sure! Can anyone tell me if there is a federal law regarding this? We no longer use B5, we use B+, but I know someone in OK who is using B5 (I am in FL). Is he breaking any laws by using it and/or should he be switched to an alternative like B+? Thanks! Michelle ------------------------------ Message: 12 Date: Thu, 6 May 2010 11:16:04 -0400 From: "Bartlett, Jeanine (CDC/OID/NCZVED)" Subject: [Histonet] myocyte damage To: histonet Message-ID: <68510B12184E45498EABD4CB6F3868FE012870BC@LTA3VS001.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii Hello everyone, I am in need of a special stain or an IHC that will demonstrate myocyte damage in muscle tissue...esp. cardiac. Any help will be greatly appreciated. Thanks! Jeanine Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov ------------------------------ Message: 13 Date: Thu, 6 May 2010 10:27:50 -0500 From: tonia.richmond@gracepathology.com Subject: RE: [Histonet] Responses to IHC CAP Validation question To: "McMahon, Loralee A" Cc: "histonet@lists.utsouthwestern.edu" , "thisisann@aol.com" Message-ID: Content-Type: text/plain; charset="UTF-8" If you are a brand new lab, how do you validate IHC if you are no yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer Grace Pathology PH: (501) 765-7367 Email: -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- < To: "thisisann@ "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" Subject: [Histonet] Starting up new lab To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I'm looking at starting up a new histology lab and need everything, I have a tight budget. Can anybody give me recommendations on where to buy refurbished equipment. Thanks Sharon ------------------------------ Message: 15 Date: Thu, 06 May 2010 11:57:43 -0400 From: "Richard Cartun" Subject: [Histonet] Formalin fixation time for breast specimens To: "Histonet" Message-ID: <4BE2AEB7.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII There has been a lot of discussion recently regarding recommendations for formalin fixation of breast specimens. If you are interested in this topic please read the following article published in the May 2010 issue of the American Journal of Clinical Pathology by Ibarra JA, et al., "Fixation time does not affect the expression of estrogen receptor". Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 16 Date: Thu, 6 May 2010 12:16:45 -0400 From: BSullivan@shorememorial.org Subject: RE: [Histonet] Responses to IHC CAP Validation question To: tonia.richmond@gracepathology.com Cc: "histonet@lists.utsouthwestern.edu" , "McMahon, Loralee A" , histonet-bounces@lists.utsouthwestern.edu, "thisisann@aol.com" Message-ID: Content-Type: text/plain; charset=US-ASCII One important thing to remember is that you should make sure All material being used for validation is processed the same way. Will your control tissue be purchased or processed in your lab? Control tissue is used for validation but you should use tissue with various levels of positivity. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 tonia.richmond@gr acepathology.com Sent by: To histonet-bounces@ "McMahon, Loralee A" lists.utsouthwest cc "histonet@lists.utsouthwestern.edu" 05/06/2010 11:27 AM , "thisisann@aol.com" Subject RE: [Histonet] Responses to IHC CAP Validation question If you are a brand new lab, how do you validate IHC if you are no= t yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer = / Laboratory Grace Pathology PH: (501) 765-7367 Email: = [1]tonia.= richmond@gracepathology.com -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- <= /FONT> To: "thisisann@= aol.com" , "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" Sent by: histonet-bounces@lists.u= tsouthwestern.edu Date: 04/28/2010 02:01PM Subject: RE: [Histonet] Re= sponses to IHC CAP Validation question Any inspection that I have under= gone we have used the 25 to 30 case rule. Except for the Er/Pr//Her-2= . We use closer to 50 cases. We also use a TMA to make our live= s easier. The TMA contains known positives and known negatives. I= n cases of t-cell or b-cell markers or cytokeratins. 25 to 30 cases i= s easy. But when you are validated for more hard to find markers (SV-= 40) then fewer cases is acceptable. We always throw in a slide that w= e know will not stain for sv-40 like a tonsil - then you can say it has spe= cificity. Any inspector that I have come across is usually understanding= of this. But I am sure that there are exceptions to this.........esp ecially if they are not familiar with immunohistochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong M= emorial Hospital Department of Surgical Pathology (585) 275-7210 ______________________ 5F__= _______________ From: histonet-bounces@lis= ts.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf= Of thisisann@aol.com [thisisann@aol.com] Sent: Wednesday, April 28, 201= 0 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] R= esponses to IHC CAP Validation question The following is one respone= I rec'd: 1. I asked CAP who told me that they do not currentl= y have a guideline on validating but that they recommend what is in t= he following book: Quality Management In Anatomic Pathology, Promoting P= atient Safety Through Systems Improvement and Error by Raouf E. Nakhl= eh, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8-= Quality Management in IHC That is what we follow. I. Get a new antib= ody and optimize it with your positive control. II. Once optimized you n= eed to run it on cases expected to be positive (how many?) "a suffien= t size ..." III. Must also be run on cases expected to be negative. (how= many? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just = that. (ex. some of the hormones we just use a pituitary) ___________________ ______________________ 5F__= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu = [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________ 5F__ ______________________ Histo= net mailing list Histonet@lists.utsouthwestern.edu [3]http://l= ists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:tonia.richmond@gracepathology.com" 2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 3. 3D"http://=/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 06 May 2010 12:30:50 -0400 From: Merced M Leiker Subject: Re: [Histonet] myocyte damage To: "Bartlett, Jeanine (CDC/OID/NCZVED)" , histonet Message-ID: <5AFA0A6D83365A1F044D4E16@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Stain with cTnI and inspect for damage visually. Just a guess. Regards, Merced --On Thursday, May 06, 2010 11:16 AM -0400 "Bartlett, Jeanine (CDC/OID/NCZVED)" wrote: > Hello everyone, > > I am in need of a special stain or an IHC that will demonstrate myocyte > damage in muscle tissue...esp. cardiac. > > Any help will be greatly appreciated. > > Thanks! > Jeanine Bartlett > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 7 *************************************** From Karen.Heckford <@t> CHW.edu Tue May 11 07:15:40 2010 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Tue May 11 07:15:44 2010 Subject: [Histonet] Are Histotechs considered "exempt" employees? In-Reply-To: References: Message-ID: <2842DC75AE43AA4B92954CFB31781BC105697FA5@CHW-MSG-301.chw.edu> $16.15 per hour. They are getting a great deal. I believe you should be making at least double that. What I believe is happening is that a lot of offices are now starting to open their own labs and they are trying to get us cheap. We all need to stick to our guns and not let them get us cheaper than we are worth. Yes, I realize that San Francisco is more but the starting wage for a HT's should be $25.00 to $30.00 an hour to start for a newly certified tech. No matter of location in California. You can always negotiate higher if you have more experience and more certifications. Just my two cents worth, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Sandoval Sent: Monday, May 10, 2010 9:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Histotechs considered "exempt" employees? Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy_schmitt <@t> pa-ucl.com Tue May 11 07:19:26 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue May 11 07:19:41 2010 Subject: [Histonet] RE: Histonet Digest, Vol 78, Issue 12 In-Reply-To: <20100510174202.1456F127164@mail.pa-ucl.com> References: <20100510174202.1456F127164@mail.pa-ucl.com> Message-ID: <737BD0BF52F0744B96B74B61756AC0644166A09A91@hestia.ad.pa-ucl.com> Hi Jason We cut 200+ a day - we have histo tech in at 400, 430, 500 and 2 at 6am. One is student in training, and one is on the brink of retirement, so there will be only 1 coming in at 6. I am the coordinator the dept. so after cutting is finished, and if we are at full staff, I then switch to desk work. Nancy Schmitt HT, MLT (ASCP) Histology Coordinator Dubuque, IA Message: 3 Date: Mon, 10 May 2010 08:43:30 -0400 From: "Jason Keller" Subject: [Histonet] Number of Techs To: Message-ID: <7D41E97C787DF44996E0055C81703663F17308@ntexchange3.capefear.local> Content-Type: text/plain; charset="us-ascii" Hello, I am looking to compile information on the average number of techs that different hospitals have in their histology labs. I would appreciate any feedback that I could get as far as how many techs are used to run hospital histology labs with a block count range of 200 to 400 blocks per day. Thanks for any input you can provide, Jason NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From HornHV <@t> archildrens.org Tue May 11 08:38:49 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue May 11 08:40:11 2010 Subject: [Histonet] Specimen Delivery In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8381E@EMAIL.archildrens.org> We have a central drop off area. Occasionally we will have shared specimens with microbiology and shared fluid specimens with the clinical lab. This helps ensure all tests ordered are performed. We pick up our specimens from the central area. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mwhite@mcleodhealth.org Sent: Monday, May 10, 2010 1:35 PM To: histonet@lists.utsouthwestern.edu Cc: TBailey@mcleodhealth.org Subject: [Histonet] Specimen Delivery For those of you who do tissue/cytology processing in a hospital or similar facility: Are tissue or fluid specimens brought directly to the Anatomic Pathology/Cytology area, or are specimens dropped off in a clinical accessioning area ? We are debating the pros/cons of having our specimens dropped at a centralized location since our Lab is becoming more "spread out" and nurses are confused about where to bring stuff. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From micropathlabs <@t> yahoo.com Tue May 11 08:41:49 2010 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue May 11 08:41:58 2010 Subject: [Histonet] Cytotech Position Message-ID: <835784.30336.qm@web57804.mail.re3.yahoo.com> Hi everyone!?If anyone knows a?cytotechnologist interested in a position, we have one here in Lakeland,?Florida. It is a full-time position, M-F and candidate must have FL state license or be eligible to obtain one. Please?contact me for further information. Thank you in advance. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From Kim.Donadio <@t> bhcpns.org Tue May 11 09:03:53 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue May 11 09:04:47 2010 Subject: [Histonet] Are Histotechs considered "exempt" employees? In-Reply-To: <2842DC75AE43AA4B92954CFB31781BC105697FA5@CHW-MSG-301.chw.edu> Message-ID: Starting out at $16 an hour is not acceptable. I've hired recently graduated students at a better pay than that. And this is Florida where the cost of living is a lot cheaper than Cali. Hope this helps. :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Heckford, Karen - SMMC-SF" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/11/2010 07:15 AM To "Anthony Sandoval" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] Are Histotechs considered "exempt" employees? $16.15 per hour. They are getting a great deal. I believe you should be making at least double that. What I believe is happening is that a lot of offices are now starting to open their own labs and they are trying to get us cheap. We all need to stick to our guns and not let them get us cheaper than we are worth. Yes, I realize that San Francisco is more but the starting wage for a HT's should be $25.00 to $30.00 an hour to start for a newly certified tech. No matter of location in California. You can always negotiate higher if you have more experience and more certifications. Just my two cents worth, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Sandoval Sent: Monday, May 10, 2010 9:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Histotechs considered "exempt" employees? Hello fellow Histotechs, I have recently become certified as an HTL and was wondering if anyone out there is an 'exempt' employee? I live in California and feel that I am being taken advantage of. I make 16.15$ per hour and frequently work 50 hour weeks. Am I off base? should I just be grateful that I have a job, as my employer so frequently reminds me? Thank you Histonet! you have been an invaluable resource in my career and assisting me in passing the HTL! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From cgill <@t> marylandgeneral.org Tue May 11 09:17:26 2010 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Tue May 11 09:17:40 2010 Subject: [Histonet] per deim work In-Reply-To: <629474.84559.qm@web36103.mail.mud.yahoo.com> References: <629474.84559.qm@web36103.mail.mud.yahoo.com> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9E93@MDGEN-EXCH1.marylandgeneral.org> Have you tried Aerotech scientific(sp). I know they are in several states you should look them up in your area. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of richard shook Sent: Monday, May 10, 2010 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] per deim work Hello Histoland I have question pertaining to temporary histology tech work/per Diem positions.I find myself currently out of work and am looking for my next adventure in? the great world of histology and one of the ideas i had was to check out the possibility's of doing temp work.The only thing is I'm not sure if there is a temp agency for histo techs,or is there someone in histo land that currently working as a temp . I would greatly appreciate any help in my endeavors into this new world. Any information as to how much the pay rate or what?to charge for daily work travel?lodging or any other expenses that would be incurred in this?type of work. Thanks for any help Richard Shook HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Tue May 11 09:34:30 2010 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Tue May 11 09:34:44 2010 Subject: [Histonet] Are Histotechs considered "exempt" employees? In-Reply-To: References: Message-ID: Anthony, You live in CA, work 50 hour weeks and you are a HTL with an unprecedented and valuable resource at your disposal, Histonet and you know how to use it. For this you get paid $16.15/hour by what appears to be an employer who isn't grateful to have you. I'd start looking for another job. Just my humble opinion. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote: > Hello fellow Histotechs, I have recently become certified as an HTL > and was wondering if anyone out there is an 'exempt' employee? I > live in California and feel that I am being taken advantage of. I > make 16.15$ per hour and frequently work 50 hour weeks. Am I off > base? should I just be grateful that I have a job, as my employer so > frequently reminds me? Thank you Histonet! you have been an > invaluable resource in my career and assisting me in passing the HTL! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Karen.Heckford <@t> CHW.edu Tue May 11 10:17:15 2010 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Tue May 11 10:17:20 2010 Subject: [Histonet] Are Histotechs considered "exempt" employees? In-Reply-To: References: Message-ID: <2842DC75AE43AA4B92954CFB31781BC105697FA7@CHW-MSG-301.chw.edu> I am sooooo with you on this one. HT's need to start demanding and higher wages everywhere. We are a rare breed with special talents. Without a good tech you do not get good slides!! We are not a dime a dozen. AUGH! I know just a hour north of where I am the wages can be $10-$15.00 less. Not much difference in living expenses either. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, May 11, 2010 7:35 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Are Histotechs considered "exempt" employees? Anthony, You live in CA, work 50 hour weeks and you are a HTL with an unprecedented and valuable resource at your disposal, Histonet and you know how to use it. For this you get paid $16.15/hour by what appears to be an employer who isn't grateful to have you. I'd start looking for another job. Just my humble opinion. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote: > Hello fellow Histotechs, I have recently become certified as an HTL > and was wondering if anyone out there is an 'exempt' employee? I > live in California and feel that I am being taken advantage of. I > make 16.15$ per hour and frequently work 50 hour weeks. Am I off > base? should I just be grateful that I have a job, as my employer so > frequently reminds me? Thank you Histonet! you have been an > invaluable resource in my career and assisting me in passing the HTL! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue May 11 10:37:14 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue May 11 10:37:32 2010 Subject: [Histonet] need tips for cross-sectioning of cortical bone In-Reply-To: References: <63EA0607835FBA4689CEA9EA8B48269202FFDF8D@usctmx1141.merck.com> Message-ID: <002c01caf11f$d5dcd4a0$81967de0$@callis@bresnan.net> You did not say whether these are cross sections or mid sagittal? Cross sections are always tougher. Key is to make sure the cortical bone is well processed and infiltrated with a hard paraffin e.g. Tissue Prep 2 (Fisher Scientific ala Thermo Scientific). Try this old bonehead trick is cut tiny V-shaped notches with razor blade or used microtome blade on the sides of block, parallel to the blade. You may have to do this top and bottom too. Take care to NOT make these notches huge. This permits the paraffin of each section in ribbon to expand, relax onto the water bath along with the bone section. Another trick is try laying section on RT 5 to 10% alcohol, pick up on slide, then go to warm water bath, lower section slowly to flattening. The key here is to NOT let upper part of paraffin of a section totally release from slide while going into warm water (at an angle) in other words, the section flattens while paraffin portion is still attached to slide during flattening. Also, change the blade frequently. Sharpest possible edge helps, and hopefully high profile which is more stable than low profile for decalcified bone microtomy. Good luck Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Tuesday, May 11, 2010 5:03 AM To: Connolly, Brett M Cc: Subject: Re: [Histonet] need tips for cross-sectioning of cortical bone Why not embed in resin (MMA) and take thicker sections and then grind/ polish them down? If you went this route, you could then use flourescent labels and quantify mineral apposition rate and bone formation rate. Let me know if you are interested. I can help you get started and direct you to low cost equipment options. Jack On Apr 22, 2010, at 9:58 AM, "Connolly, Brett M" wrote: > A colleague is having trouble getting wrinkle-free sections of > decalcified, paraffin embedded femur. > > Any tips?? > > Thanks, > > Brett M. Connolly, Ph.D. > Molecular Imaging Team Leader > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > tel. 215-652-2501 fax. 215-993-6803 > brett_connolly@merck.com > > > > Notice: This e-mail message, together with any attachments, > contains information of Merck & Co., Inc. (One Merck Drive, > Whitehouse Station, New Jersey, USA 08889), and/or its affiliates > Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html > ) that may be confidential, proprietary copyrighted and/or legally > privileged. It is intended solely for the use of the individual or > entity named on this message. If you are not the intended recipient, > and have received this message in error, please notify us > immediately by reply e-mail and then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5103 (20100510) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5103 (20100510) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5105 (20100511) __________ The message was checked by ESET Smart Security. http://www.eset.com From liz <@t> premierlab.com Tue May 11 11:13:35 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue May 11 11:13:40 2010 Subject: [Histonet] article on Er/PR validation Message-ID: Hello everyone I have been trying to locate this article on line and I'm either challenged or its not there. Its from the Arch Pathol Lab Med (in press) it says in press so I don't know what that that means but its not available off the Journals web site Fitzgibbons, P. L. , D. A. Murphy , M. E. H. Hammond , et al. Recommendations for validating estrogen and progesterone receptor immunohistochemistry assays. Arch Pathol Lab Med (in press). Does anyone have a copy of this that they are willing to forward to me, the new ER/PR guidelines that just came out reference this article for validation, even through at the end of the article there is a statement --- "there is no universal acceptable procedure for validating tests" any help would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From liz <@t> premierlab.com Tue May 11 11:18:37 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue May 11 11:18:47 2010 Subject: [Histonet] Article for Er/PR vaidation Message-ID: Hello everyone I have been trying to locate this article on line and I'm either challenged or its not there. Its from the Arch Pathol Lab Med (in press) it says in press so I don't know what that that means but its not available off the Journals web site Fitzgibbons, P. L. , D. A. Murphy , M. E. H. Hammond , et al. Recommendations for validating estrogen and progesterone receptor immunohistochemistry assays. Arch Pathol Lab Med (in press). Does anyone have a copy of this that they are willing to forward to me, the new ER/PR guidelines that just came out reference this article for validation, even through at the end of the article there is a statement --- "there is no universal acceptable procedure for validating tests" any help would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From cbrya <@t> lexclin.com Tue May 11 11:58:37 2010 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Tue May 11 11:58:42 2010 Subject: [Histonet] processing/cutting cell blocks Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF0595E9619E@EXCHANGESB> We typically use a media called histogel for our cytology cell blocks with very good results. Our pathologists also send us clots from other locations that they are collecting like a bone marrow aspirate clot. The FNAs are expressed into a lid and allowed to clot. We are processing these on an overnight run used for all our other cases. The histotechs are having a great deal of trouble cutting these as they are very dried out and break apart on the water bath. Is there any techniques you can recommend to get better results when cutting specimens collected this way? I would think we need to process them on a shorter program to begin with. Thank you in advance for any suggestions. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From brett_connolly <@t> merck.com Tue May 11 12:37:58 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue May 11 12:38:13 2010 Subject: [Histonet] Thanks for bone cutting suggestions - and anti-Rat CD3 Message-ID: <63EA0607835FBA4689CEA9EA8B48269203126435@usctmx1141.merck.com> Thanks to all for your suggestions for cutting cross sections of cortical bone. I have passed them along. Now, what is the latest on CD3 IHC in FFPE rat tissues. I saw Ray Koelling's suggestion in the archives using an Ab from BD Biosciences. Is it cat # 550295? What others if any do you suggest? HEIR ? protocol tips? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From wdesalvo.cac <@t> hotmail.com Tue May 11 13:00:56 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue May 11 13:01:15 2010 Subject: [Histonet] Forwarding Request for Control Mehtod Message-ID: > I wonder if you could help me find someone who would kindly donate a > paraffin tissue block containing candida or other fungi to be used as a > control block. We have had difficulty in obtaining such tissue and have > tried all the hospitals in northern Ireland for a tissue block suitable > enough to act as a control. Many of the pathology departments are in the > same position as ourselves in that the control tissue being used is > getting in short supply. > > I tried to make one using a culture from microbiology with fresh lambs > lung from a local abattoir but unfortunately the tissue seems to stain > with PAS as well and the candida where obscured although visible > probably due to post mortem changes in the tissue. > Alternatively have any of your members successfully produced fungal > control material by other means. > > Thanking you in advance > Ian Clarke > Biomedical Scientist > Cellular Pathology Department > Craigavon Area Hospital > Sothern Health and Social Services Trust. > 66 Lurgan Road > Craigavon > BT63 5QQ > Northern Ireland William DeSalvo, B.S., HTL(ASCP) _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4 From liz <@t> premierlab.com Tue May 11 13:32:00 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue May 11 13:32:04 2010 Subject: [Histonet] Thanks for bone cutting suggestions - and anti-Rat CD3 In-Reply-To: <63EA0607835FBA4689CEA9EA8B48269203126435@usctmx1141.merck.com> Message-ID: Dako has a rabbit polyclonal that works in rat. The other thing with the bone, is place the slide with a section on a flat hotplate (60 C) until it turns clear - not too long or the articular cartilage will flip. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, May 11, 2010 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thanks for bone cutting suggestions - and anti-Rat CD3 Thanks to all for your suggestions for cutting cross sections of cortical bone. I have passed them along. Now, what is the latest on CD3 IHC in FFPE rat tissues. I saw Ray Koelling's suggestion in the archives using an Ab from BD Biosciences. Is it cat # 550295? What others if any do you suggest? HEIR ? protocol tips? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brandihiggins <@t> gmail.com Tue May 11 13:55:20 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Tue May 11 13:55:27 2010 Subject: [Histonet] CYP.07680 - cytology cross contamination Message-ID: Hello all, For CAP policy CYP.07680 for "procedures to prevent cross-contamination of specimens during processing and staining" - what are your labs doing? I work in a small lab so the histotechs process the cytology specimens and the pathologists read the slides (we have no PA's or cytoprep techs or cytotechs to screen slides). We also process only non-GYN, so we don't have to worry about GYN/non-GYN cross contamination. The notes under this policy say procedures must prevent cross-contamination between highly cellular specimens and suggest the screening method of toluidine blue stain to determing if specimens are highly cellular. Does anyone use the toluidine blue for this purpose? If so could you tell me the procedure for toluidine blue you use? And how do you determing which specimens you stain with toluidine blue and what qualifies as highly cellular. If so do you retain these toluidine blue slides for any period of time? CAP policy also suggests inserting a clean blank slide in each stain run and examine for contamination. Is anyone doing this? We have been inspected before with no problems with this CAP question, but I just want to make sure we are doing everything we can to prevent cross-contamination. Thanks in advance for your input! Brandi Higgins, HT (ASCP) From gankam <@t> googlemail.com Tue May 11 14:06:21 2010 From: gankam <@t> googlemail.com (Fabrice gankam) Date: Tue May 11 14:06:26 2010 Subject: [Histonet] L-DNAse II vs neutrophil (leukocyte) elastase inhibitor Message-ID: Hi guys Would like to know if any of you have used the L DNAse II or the serpin B1 antibody in the rat. very few references mentioning this antibody. Sigma has one prestige antibody for serpin B1 but do no know if it will work on rats. I was wondering if the DNAse II is the equivalent of the L DNAse II (product of the degradation of serpin) thanks for your help From T.vanderAa <@t> sakura.eu Tue May 11 14:14:24 2010 From: T.vanderAa <@t> sakura.eu (Toine van der Aa) Date: Tue May 11 14:14:55 2010 Subject: [Histonet] Genemed antibodies Message-ID: Hi, Does anybody have experience with Genemed Biotechnologies antibodies and/or probes of Histosonda? Kind regards, A.Van der Aa ________________________________ Confidentiality Note: This information and the attached file(s) are considered trade secret, confidential and/or property by Sakura Finetek Europe B.V. Any use, disclosure of reproduction of these documents by anyone other than the addressee is prohibited. If this E-mail has been received by anyone other than the addressee, please call +31 (0)88 592 00 00 immediately to arrange for the document(s) to be returned. Sakura can never be legally bound by the acceptance of any supposed offer in an electronic message. Please reply or send your e-mail to: Sakura@sakura.eu Sakura Finetek Europe B.V. P.O. Box 362, 2400 AJ Flemingweg 10A, 2408 AV Alphen aan den Rijn The Netherlands Tel. +31 (0)88 592 00 00 Fax +31 (0)88 592 00 01 KvK/Chamber of Commerce Leiden 28065449 From sgoebel <@t> xbiotech.com Tue May 11 14:16:33 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue May 11 14:17:03 2010 Subject: [Histonet] ICC Message-ID: <20100511121633.9e2d9aa830e8449a2412eb1e4f2f067e.6c1d241588.wbe@email04.secureserver.net> Hello all, I am starting ICC and flourescence for the first time. I have done tons of IHC in the past with DAB and AEC slightly confused with the way the flourescence works. Do you have to buy a secondary that is already tagged, or is system where it attaches to the secondary like DAB does? anyone have any advice or some site where I can go to get some info on this. Also, what do you use for positive and negative controls fo r ICC? Thanks fellow histo hotties!! [DEL: Sarah Histotechni XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 From brent.jeter <@t> gwu-hospital.com Tue May 11 15:33:12 2010 From: brent.jeter <@t> gwu-hospital.com (Jeter, Brent) Date: Tue May 11 15:33:28 2010 Subject: [Histonet] CYP.07680 - cytology cross contamination In-Reply-To: References: Message-ID: Hi Brandi, The clean, blank slide is some times inserted between cases in the staining rack to determine if floaters are present. It's sort of a retroactive test - if you see cells on the blank slide, then you already have cross-contamination and you need to re-prep and restain the cases separately. Some people feel that you've caught the floaters on your clean slide and the problem is solved, but there's no guarantee others won't be present on your specimen slides, too. Toluidine blue is an example of a stain you could use to determine cellularity; another option often used is a modified Wright Giemsa stain such as Diff-Quik. You're not looking to make a final diagnosis, but to make a quick preparation and use a simple stain just to see if there are a lot of cells present. This is a more proactive test. The idea is that highly cellular specimens have an increased likelihood of shedding cells into the stains and contaminating other cases; it also can be used to identify obviously malignant cases, which should definitely be stained separately. If you're using a rapid stain to look for high cellularity, you need to centrifuge and concentrate the specimen(s) first, just as you would in regular prep. The Diff-Quik stain is a Romanovsky stain and is used on air-dried slides, so there's no need for fixation. Hope that helps - Brent Jeter Anatomic Pathology Supervisor The George Washington University Hospital 202-715-5076 (phone) 202-715-4691 (fax) brent.jeter@gwu-hospital.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins [brandihiggins@gmail.com] Sent: Tuesday, May 11, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CYP.07680 - cytology cross contamination Hello all, For CAP policy CYP.07680 for "procedures to prevent cross-contamination of specimens during processing and staining" - what are your labs doing? I work in a small lab so the histotechs process the cytology specimens and the pathologists read the slides (we have no PA's or cytoprep techs or cytotechs to screen slides). We also process only non-GYN, so we don't have to worry about GYN/non-GYN cross contamination. The notes under this policy say procedures must prevent cross-contamination between highly cellular specimens and suggest the screening method of toluidine blue stain to determing if specimens are highly cellular. Does anyone use the toluidine blue for this purpose? If so could you tell me the procedure for toluidine blue you use? And how do you determing which specimens you stain with toluidine blue and what qualifies as highly cellular. If so do you retain these toluidine blue slides for any period of time? CAP policy also suggests inserting a clean blank slide in each stain run and examine for contamination. Is anyone doing this? We have been inspected before with no problems with this CAP question, but I just want to make sure we are doing everything we can to prevent cross-contamination. Thanks in advance for your input! Brandi Higgins, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. From steve <@t> myelinrepair.org Tue May 11 15:53:31 2010 From: steve <@t> myelinrepair.org (Steve Wong) Date: Tue May 11 15:48:02 2010 Subject: [Histonet] Labomed LX400 compound microscope Message-ID: Hello, Does anyone have any experience with that Labomed Lx 400 compound microscope? It is new and it is cheap (scope with Moticam 2000 camera - ~$2,000), but I cannot find any reviews on it. I'm looking for a simple microscope to take digital pictures of cross sections of rat spinal cords for some volumetric measurements. Steve From AnthonyH <@t> chw.edu.au Tue May 11 19:53:48 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue May 11 19:54:11 2010 Subject: [Histonet] Forwarding Request for Control Mehtod In-Reply-To: Message-ID: Ian, What I have done in the past is to raid the Staff Fridge (or your son's bedroom!). There is usually old food left there that has gone moldy - bread or even strawberries. Wearing gloves & in a fume hood slice the bread or fruit into slices (ensuring visible growth of fungi is present), fix for two or more days in NBF, process as usual. Voila - fungi controls Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, 12 May 2010 4:01 AM To: histonet Subject: [Histonet] Forwarding Request for Control Mehtod > I wonder if you could help me find someone who would kindly donate a > paraffin tissue block containing candida or other fungi to be used as > a control block. We have had difficulty in obtaining such tissue and > have tried all the hospitals in northern Ireland for a tissue block > suitable enough to act as a control. Many of the pathology departments > are in the same position as ourselves in that the control tissue being > used is getting in short supply. > > I tried to make one using a culture from microbiology with fresh lambs > lung from a local abattoir but unfortunately the tissue seems to stain > with PAS as well and the candida where obscured although visible > probably due to post mortem changes in the tissue. > Alternatively have any of your members successfully produced fungal > control material by other means. > > Thanking you in advance > Ian Clarke > Biomedical Scientist > Cellular Pathology Department > Craigavon Area Hospital > Sothern Health and Social Services Trust. > 66 Lurgan Road > Craigavon > BT63 5QQ > Northern Ireland William DeSalvo, B.S., HTL(ASCP) _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PI D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___________________________ ____________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From kimtournear <@t> yahoo.com Tue May 11 21:24:49 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue May 11 21:24:53 2010 Subject: [Histonet] Are Histotechs considered "exempt" employees? In-Reply-To: <2842DC75AE43AA4B92954CFB31781BC105697FA7@CHW-MSG-301.chw.edu> Message-ID: <235254.69116.qm@web54207.mail.re2.yahoo.com> Well said Andi!! ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! --- On Tue, 5/11/10, Heckford, Karen - SMMC-SF wrote: From: Heckford, Karen - SMMC-SF Subject: RE: [Histonet] Are Histotechs considered "exempt" employees? To: "Andrea Grantham" Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, May 11, 2010, 8:17 AM I am sooooo with you on this one.???HT's need to start demanding and higher wages everywhere.? We are a rare breed with special talents. Without a good tech you do not get good slides!!? We are not a dime a dozen.? AUGH!? I know just a hour north of where I am the wages can be $10-$15.00 less. Not much difference in living expenses either. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, May 11, 2010 7:35 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Are Histotechs considered "exempt" employees? Anthony, You live in CA, work 50 hour weeks and you are a HTL with an? unprecedented and valuable resource at your disposal, Histonet and you? know how to use it. For this you get paid $16.15/hour by what appears? to be an employer who isn't grateful to have you. I'd start looking? for another job. Just my humble opinion. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415? ???Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" -? Paula Sicurello P Please consider the environment before printing this email. On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote: > Hello fellow Histotechs, I have recently become certified as an HTL? > and was wondering if anyone out there is an 'exempt' employee? I? > live in California and feel that I am being taken advantage of.? I? > make 16.15$ per hour and frequently work 50 hour weeks. Am I off? > base? should I just be grateful that I have a job, as my employer so? > frequently reminds me? Thank you Histonet! you have been an? > invaluable resource in my career and assisting me in passing the HTL! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gankam <@t> googlemail.com Tue May 11 21:25:45 2010 From: gankam <@t> googlemail.com (Fabrice GANKAM) Date: Tue May 11 21:26:01 2010 Subject: [Histonet] RE: L-DNAse II vs neutrophil (leukocyte) elastase inhibitor In-Reply-To: References: Message-ID: <3CC647ECDCDC41E1BC06895D398ABF38@PCdeGANKAM> Hi guys Would like to know if any of you have used the L DNAse II or the serpin B1 antibody in the rat. very few references mentioning this antibody. Sigma has one prestige antibody for serpin B1 but do no know if it will work on rats. I was wondering if the DNAse II is the equivalent of the L DNAse II (product of the degradation of serpin) thanks for your help From emmanuelg <@t> gmail.com Wed May 12 02:20:21 2010 From: emmanuelg <@t> gmail.com (Emmanuel Gonz) Date: Wed May 12 02:21:05 2010 Subject: [Histonet] RE: Are Histotechs considered "exempt" employees? Message-ID: >From what i know and heard, your facility is definitely getting a great deal. It really depends on the facility. The lab i work at starts non-certified techs around 20. I know of a small derm lab that start non-certified techs around 18. Emmanuel Gonzaga HT(ASCP) From ian.montgomery <@t> bio.gla.ac.uk Wed May 12 06:31:37 2010 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed May 12 06:34:19 2010 Subject: FW: [Histonet] Forwarding Request for Control Mehtod Message-ID: Tony, Is this a universal teenage thing, filthy bedrooms? I'm convinced there are alien life forms in my daughter's bedroom. They make the strangest noises, grunts and groan and "I'm bored." Me, I was the perfect teenager apart from the Celtic thing of taking to the drink and of course, lusting after girls. Ian. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 12 May 2010 01:54 To: WILLIAM DESALVO; histonet Subject: RE: [Histonet] Forwarding Request for Control Mehtod Ian, What I have done in the past is to raid the Staff Fridge (or your son's bedroom!). There is usually old food left there that has gone moldy - bread or even strawberries. Wearing gloves & in a fume hood slice the bread or fruit into slices (ensuring visible growth of fungi is present), fix for two or more days in NBF, process as usual. Voila - fungi controls Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, 12 May 2010 4:01 AM To: histonet Subject: [Histonet] Forwarding Request for Control Mehtod > I wonder if you could help me find someone who would kindly donate a > paraffin tissue block containing candida or other fungi to be used as > a control block. We have had difficulty in obtaining such tissue and > have tried all the hospitals in northern Ireland for a tissue block > suitable enough to act as a control. Many of the pathology departments > are in the same position as ourselves in that the control tissue being > used is getting in short supply. > > I tried to make one using a culture from microbiology with fresh lambs > lung from a local abattoir but unfortunately the tissue seems to stain > with PAS as well and the candida where obscured although visible > probably due to post mortem changes in the tissue. > Alternatively have any of your members successfully produced fungal > control material by other means. > > Thanking you in advance > Ian Clarke > Biomedical Scientist > Cellular Pathology Department > Craigavon Area Hospital > Sothern Health and Social Services Trust. > 66 Lurgan Road > Craigavon > BT63 5QQ > Northern Ireland William DeSalvo, B.S., HTL(ASCP) _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PI D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___________________________ ____________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **************************************************************************** ***** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Wed May 12 07:28:00 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed May 12 07:28:18 2010 Subject: FW: [Histonet] Forwarding Request for Control Mehtod In-Reply-To: References: Message-ID: <4BEA6690.59CD.00EE.0@hurleymc.com> If you have one of those great automatic stainers that has a drain tube going into a sink, you will also find that, after some time, a wonderful fungus will grow inside of the tubing!! We remove the tubing and shake the fungus loose. Counter staining isn't the best on this type of specimen, but when you're in a pinch, you will definitely obtain positive fungus staining! And, wherever you are, it must be warmer/dryer than Michigan right now..........please send heat!!! hope this helped! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 >>> "Ian Montgomery" 5/12/2010 7:31 AM >>> Tony, Is this a universal teenage thing, filthy bedrooms? I'm convinced there are alien life forms in my daughter's bedroom. They make the strangest noises, grunts and groan and "I'm bored." Me, I was the perfect teenager apart from the Celtic thing of taking to the drink and of course, lusting after girls. Ian. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 12 May 2010 01:54 To: WILLIAM DESALVO; histonet Subject: RE: [Histonet] Forwarding Request for Control Mehtod Ian, What I have done in the past is to raid the Staff Fridge (or your son's bedroom!). There is usually old food left there that has gone moldy - bread or even strawberries. Wearing gloves & in a fume hood slice the bread or fruit into slices (ensuring visible growth of fungi is present), fix for two or more days in NBF, process as usual. Voila - fungi controls Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, 12 May 2010 4:01 AM To: histonet Subject: [Histonet] Forwarding Request for Control Mehtod > I wonder if you could help me find someone who would kindly donate a > paraffin tissue block containing candida or other fungi to be used as > a control block. We have had difficulty in obtaining such tissue and > have tried all the hospitals in northern Ireland for a tissue block > suitable enough to act as a control. Many of the pathology departments > are in the same position as ourselves in that the control tissue being > used is getting in short supply. > > I tried to make one using a culture from microbiology with fresh lambs > lung from a local abattoir but unfortunately the tissue seems to stain > with PAS as well and the candida where obscured although visible > probably due to post mortem changes in the tissue. > Alternatively have any of your members successfully produced fungal > control material by other means. > > Thanking you in advance > Ian Clarke > Biomedical Scientist > Cellular Pathology Department > Craigavon Area Hospital > Sothern Health and Social Services Trust. > 66 Lurgan Road > Craigavon > BT63 5QQ > Northern Ireland William DeSalvo, B.S., HTL(ASCP) _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PI D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___________________________ ____________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **************************************************************************** ***** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m0702936 <@t> sgul.ac.uk Wed May 12 07:34:11 2010 From: m0702936 <@t> sgul.ac.uk (Krish Soni) Date: Wed May 12 07:34:30 2010 Subject: [Histonet] Servicing of Leica equipment in the UK Message-ID: We are a histology lab based in the UK. We have a Leica CV5000 coverslipper and a Leica Autostainer XL. We are trying to find an independent company or operator (other than Leica) than can service our Leica equipment for us. Does anyone know a company or ex Leica engineer we could call? Thanks, Chris m0702936@sgul.ac.uk From DKBoyd <@t> chs.net Wed May 12 07:42:08 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed May 12 07:42:25 2010 Subject: [Histonet] CYP.07680 - cytology cross contamination In-Reply-To: Message-ID: Brandi, Body effusions have a high potential for cross contamination during the staining procedure. Body fluids ie: pleural fluid, peritoneal fluid, etc. For that reason they are not stained with other non-gyn specimens ie: bronch. wash, esoph. brush, CSF etc. Also after the staining all solutions up to the 95% before the OG-6 are dumped and refilled. The Hematoxylin, OG-6 and EA65 are filtered. The 95% after the EA-65 is dumped and alcohols are rotated up. This should prevent cross contamination. Written documentation should be your procedure and proof that you are changing/filtering solutions. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Brandi Higgins Sent by: histonet-bounces@lists.utsouthwestern.edu 05/11/2010 03:28 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] CYP.07680 - cytology cross contamination Hello all, For CAP policy CYP.07680 for "procedures to prevent cross-contamination of specimens during processing and staining" - what are your labs doing? I work in a small lab so the histotechs process the cytology specimens and the pathologists read the slides (we have no PA's or cytoprep techs or cytotechs to screen slides). We also process only non-GYN, so we don't have to worry about GYN/non-GYN cross contamination. The notes under this policy say procedures must prevent cross-contamination between highly cellular specimens and suggest the screening method of toluidine blue stain to determing if specimens are highly cellular. Does anyone use the toluidine blue for this purpose? If so could you tell me the procedure for toluidine blue you use? And how do you determing which specimens you stain with toluidine blue and what qualifies as highly cellular. If so do you retain these toluidine blue slides for any period of time? CAP policy also suggests inserting a clean blank slide in each stain run and examine for contamination. Is anyone doing this? We have been inspected before with no problems with this CAP question, but I just want to make sure we are doing everything we can to prevent cross-contamination. Thanks in advance for your input! Brandi Higgins, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From bsylinda <@t> aol.com Wed May 12 08:15:18 2010 From: bsylinda <@t> aol.com (bsylinda@aol.com) Date: Wed May 12 08:15:40 2010 Subject: [Histonet] Auto Dialer for Thermo Excelsior Message-ID: <8CCBFEC4D4344E6-C18-7D3D@webmail-d001.sysops.aol.com> Hello Histo, Is there anyone out there with any experience of installing a remote voice dialer on the processor. We have have one and think that it has possibly been fried due to a power surge. However this one was purchased years ago at Radio Shack and they no longer sell the same dialer. The dialers that I currently see are wireless, can anyone out there with experience with these wireless devices give me any suggestions. Thanks in advance, Sylinda Battle, HT (ASCP) From kmerriam2003 <@t> yahoo.com Wed May 12 08:24:06 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed May 12 08:24:13 2010 Subject: [Histonet] (no subject) Message-ID: <700229.76256.qm@web50303.mail.re2.yahoo.com> Good morning, I am trying to make some nice?FFPE cell pellet blocks, but I seem to lose a lot of cells along?the way (especially when trying to take the cells out of the tube).? We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again.? At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing.? I am losing a lot of the cells during this step, because the pellet is not quite solid.? Do you think it would be OK to let the cells air-dry a bit and then take them out for processing?? I know this goes against everything I was taught in histology, but I am really at a loss here. Anyone have any hot tips for me? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From sgoebel <@t> xbiotech.com Wed May 12 08:46:28 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed May 12 08:46:37 2010 Subject: [Histonet] Are Histotechs considered "exempt" =?UTF-8?Q?employees=3F?= Message-ID: <20100512064628.9e2d9aa830e8449a2412eb1e4f2f067e.af45b5d3d8.wbe@email04.secureserver.net> So, I had the same problem a few years ago. They are totall advantage of you. I live in Austin where I can't imagine tha cost of living is more than CA. The average here is between 20 25 an hour, sometimes higher if you can get a MOHS job at a priva derm clininc. Start looking, usually in my experience histo. jobs are everywhere, but sometimes you just have to call around. Also, usu wise) about HT histology department. Happy hunting!! Sarah Goebel, B.A., HT (ASCP) < Histo XBiotech USA Inc. 8201 East Rivers Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] Are Histotechs considered "exempt" employees? From: Kim Tournear Date: Tue, May 11, 2010 7:24 pm To: Histonet Well said Andi!! ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! --- On Tue, 5/11/10, Heckford, Karen - SMMC-SF Subject: RE: [Histonet] Are Histotechs considered "exempt" employees? To: "Andrea Grantham" Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, May 11, 2010, 8:17 AM I am sooooo with you on this one. HT's need to start deman higher wages everywhere. We are a rare breed with special talents. Without a good tech you do not get good slides!! We are not a dime a< I know just a hour north of where I am the wages can be $10-$15.00 less. Not much difference in living expenses either. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [[1]mailto:histonet-bounces@lists.utsouthwestern.e Andrea Grantham Sent: Tuesday, May 11, 2010 7:35 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Are Histotechs considered "exempt" employees? Anthony, You live in CA, work 50 hour weeks and you are a HTL with an unprecedented and valuable resource at your disposal, Histonet and you know how to use it. For this you get paid $16.15/hour by what appears to be an employer who isn't grateful to have you. I'd start looking < Just my humble opinion. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - < P Please consider the environment before printing this email. On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote: > Hello fellow Histotechs, I have recently become certified as an HTL&nb > and was wondering if anyone out there is an 'exempt' employee? I > live in California and feel that I am being taken advantage of. I > make 16.15$ per hour and frequently work 50 hour weeks. Am I off > base? should I just be grateful that I have a job, as my employer so&n > frequently reminds me? Thank you Histonet! you have been an > invaluable resource in my career and assisting me in passing the HTL!< > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu [3]http://lists.utsouthwestern.edu/mailman/listinfo/histonet< _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet< _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu [5]http://lists.utsouthwestern.edu/mailman/listinfo/histonet< References 1. file://localhost/tmp/3D"#Compose" 2. 3D"http://lists.utsouthwestern.edu/mailman/l 3. 3D"http://lists.utsouthwestern.edu/mailman/listin 4. 3D"http://lists.utsouthwestern.edu/mailman/listin 5. 3D"http://lists.utsouthwestern.edu/mailman/listin From kmerriam2003 <@t> yahoo.com Wed May 12 08:50:14 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed May 12 08:50:24 2010 Subject: [Histonet] FFPE cell pellet prep Message-ID: <491802.22615.qm@web50306.mail.re2.yahoo.com> Sorry - I forgot to put a subject line. Good morning, I am trying to make some nice?FFPE cell pellet blocks, but I seem to lose a lot of cells along?the way (especially when trying to take the cells out of the tube).? We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again.? At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing.? I am losing a lot of the cells during this step, because the pellet is not quite solid.? Do you think it would be OK to let the cells air-dry a bit and then take them out for processing?? I know this goes against everything I was taught in histology, but I am really at a loss here. Anyone have any hot tips for me? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From doakes <@t> olympicmedical.org Wed May 12 09:02:31 2010 From: doakes <@t> olympicmedical.org (Dawn Oakes) Date: Wed May 12 09:02:46 2010 Subject: [Histonet] Xylene substitutes Message-ID: What are labs using for xylene substitute. I will be using it in a hand staining setup for Mohs. Feed back on Histo-Clear, Clear solve, S-3 and formular 83. Thanks in advance. Dawn Oakes HT ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From STACEY.LANGENBERG <@t> UCDENVER.EDU Wed May 12 09:20:09 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Wed May 12 09:21:03 2010 Subject: [Histonet] Auto Dialer for Thermo Excelsior In-Reply-To: <8CCBFEC4D4344E6-C18-7D3D@webmail-d001.sysops.aol.com> References: <8CCBFEC4D4344E6-C18-7D3D@webmail-d001.sysops.aol.com> Message-ID: <593727174.233422.1273674049167.JavaMail.rim@bda2340.bisx.prod.on.blackberry> Sylinda Go to www.safemart.com and purchase the visonic ltd model dl-125. It is not wireless and works wonderful. We have both our processors hooked to it. Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: "bsylinda@aol.com" Date: Wed, 12 May 2010 07:15:18 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Auto Dialer for Thermo Excelsior Hello Histo, Is there anyone out there with any experience of installing a remote voice dialer on the processor. We have have one and think that it has possibly been fried due to a power surge. However this one was purchased years ago at Radio Shack and they no longer sell the same dialer. The dialers that I currently see are wireless, can anyone out there with experience with these wireless devices give me any suggestions. Thanks in advance, Sylinda Battle, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 12 09:33:24 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 12 09:33:39 2010 Subject: [Histonet] (no subject) In-Reply-To: <700229.76256.qm@web50303.mail.re2.yahoo.com> Message-ID: <684151.91972.qm@web65708.mail.ac4.yahoo.com> Whatever you do, do not dry them. Perhaps you could follow the same technique as cytotechs use to prepare their cell blocks. Ren? J. --- On Wed, 5/12/10, Kim Merriam wrote: From: Kim Merriam Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Wednesday, May 12, 2010, 9:24 AM Good morning, I am trying to make some nice?FFPE cell pellet blocks, but I seem to lose a lot of cells along?the way (especially when trying to take the cells out of the tube).? We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again.? At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing.? I am losing a lot of the cells during this step, because the pellet is not quite solid.? Do you think it would be OK to let the cells air-dry a bit and then take them out for processing?? I know this goes against everything I was taught in histology, but I am really at a loss here. Anyone have any hot tips for me? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed May 12 09:49:49 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed May 12 09:49:55 2010 Subject: [Histonet] (no subject) In-Reply-To: <3B0898D2D87A494BB748269D940FCF9D316398@ent-pr-xch-05.na01.crl.com> References: <700229.76256.qm@web50303.mail.re2.yahoo.com> <3B0898D2D87A494BB748269D940FCF9D316398@ent-pr-xch-05.na01.crl.com> Message-ID: <201221.63925.qm@web50306.mail.re2.yahoo.com> Thanks for the tips everyone!? I am going to order some agar (histogel) and use it!? We are all very excited around?here about this, we have been pulling our hair out over these darn?pellets. ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Cormier, Kathleen" To: Kim Merriam Sent: Wed, May 12, 2010 10:40:19 AM Subject: RE: [Histonet] (no subject) Hi Kim, Have you used Histogel? I have used it in the past (other places of employment) and on samples like yours. I just bought the gel, (not the whole unit, just the gel) scooped some out of the tube, placed into a weight boat, microwaves for a few seconds until liquid, then took a transfer pipet and dropped onto the cell pellet. I then placed the tube/pellet on a cold plate or fridge and wait until solid. I then popped out the now solid pellet, and processed as usual (no baggie/paper) in a cassette. Works like a charm.... Kathy Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.cormier@crl.com The Charles River 24th Annual Short Course on Laboratory Animal Science will be held June 21-24, 2010, in Newton, MA.? Click Here to get more information on this event. Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, May 12, 2010 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Good morning, I am trying to make some nice?FFPE cell pellet blocks, but I seem to lose a lot of cells along?the way (especially when trying to take the cells out of the tube).? We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again.? At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing.? I am losing a lot of the cells during this step, because the pellet is not quite solid.? Do you think it would be OK to let the cells air-dry a bit and then take them out for processing?? I know this goes against everything I was taught in histology, but I am really at a loss here. Anyone have any hot tips for me? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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From jl <@t> merraine.com  Wed May 12 10:17:34 2010
From: jl <@t> merraine.com (Jonah Levinger)
Date: Wed May 12 10:19:09 2010
Subject: [Histonet] RE: Histonet Digest, Vol 78, Issue 15
Message-ID: <037441DE843348DC94E3FEFFBAA7E3C2@rocklandts>

Unsubscribe me please!  Thank you 


 
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Today's Topics:

   1. Thanks for bone cutting suggestions - and anti-Rat CD3
      (Connolly, Brett M)
   2. Forwarding Request for Control Mehtod (WILLIAM DESALVO)
   3. RE: Thanks for bone cutting suggestions - and anti-Rat CD3
      (Liz Chlipala)
   4. CYP.07680 - cytology cross contamination (Brandi Higgins)
   5. L-DNAse II vs neutrophil (leukocyte) elastase inhibitor
      (Fabrice gankam)
   6. Genemed antibodies (Toine van der Aa)
   7. ICC (sgoebel@xbiotech.com)
   8. RE: CYP.07680 - cytology cross contamination (Jeter, Brent)
   9. Labomed LX400 compound microscope (Steve Wong)
  10. RE: Forwarding Request for Control Mehtod (Tony Henwood)
  11. RE: Are Histotechs considered "exempt" employees? (Kim Tournear)
  12. RE: L-DNAse II vs neutrophil (leukocyte) elastase	inhibitor
      (Fabrice GANKAM)
  13. RE: Are Histotechs considered "exempt" employees? (Emmanuel Gonz)
  14. FW: [Histonet] Forwarding Request for Control Mehtod
      (Ian Montgomery)
  15. Re: FW: [Histonet] Forwarding Request for Control Mehtod
      (Lynette Pavelich)
  16. Servicing of Leica equipment in the UK (Krish Soni)
  17. Re: CYP.07680 - cytology cross contamination (DKBoyd@chs.net)
  18. Auto Dialer for Thermo Excelsior (bsylinda@aol.com)
  19. (no subject) (Kim Merriam)
  20. RE: Are Histotechs considered "exempt" employees?
      (sgoebel@xbiotech.com)
  21. FFPE cell pellet prep (Kim Merriam)
  22. Xylene substitutes (Dawn Oakes)


----------------------------------------------------------------------

Message: 1
Date: Tue, 11 May 2010 13:37:58 -0400
From: "Connolly, Brett M" 
Subject: [Histonet] Thanks for bone cutting suggestions - and anti-Rat
	CD3
To: 
Message-ID:
	<63EA0607835FBA4689CEA9EA8B48269203126435@usctmx1141.merck.com>
Content-Type: text/plain; charset="us-ascii"

Thanks to all for your suggestions for cutting cross sections of cortical
bone. I have passed them along.

Now, what is the latest on CD3 IHC in FFPE rat tissues. I saw Ray Koelling's
suggestion in the archives using an Ab from BD Biosciences.
Is it cat # 550295?

What others if any do you suggest? HEIR ? protocol tips?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_connolly@merck.com

 
Notice:  This e-mail message, together with any attachments, contains
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notify us immediately by reply e-mail and then delete it from your system.


------------------------------

Message: 2
Date: Tue, 11 May 2010 12:00:56 -0600
From: WILLIAM DESALVO 
Subject: [Histonet] Forwarding Request for Control Mehtod
To: histonet 
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"


> I wonder if you could help me find someone who would kindly donate a 
> paraffin tissue block containing candida or other fungi to be used as 
> a control block. We have had difficulty in obtaining such tissue and 
> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology departments 
> are in the same position as ourselves in that the control tissue being 
> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh lambs 
> lung from a local abattoir but unfortunately the tissue seems to stain 
> with PAS as well and the candida where obscured although visible 
> probably due to post mortem changes in the tissue.
 
> Alternatively have any of your members successfully produced fungal 
> control material by other means.
> 
> Thanking you in advance
 
> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland


William DeSalvo, B.S., HTL(ASCP)



 		 	   		  
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------------------------------

Message: 3
Date: Tue, 11 May 2010 12:32:00 -0600
From: "Liz Chlipala" 
Subject: RE: [Histonet] Thanks for bone cutting suggestions - and
	anti-Rat CD3
To: "Connolly, Brett M" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="US-ASCII"

Dako has a rabbit polyclonal that works in rat.  The other thing with
the bone, is place the slide with a section on a flat hotplate (60 C)
until it turns clear - not too long or the articular cartilage will
flip.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Connolly, Brett M
Sent: Tuesday, May 11, 2010 11:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thanks for bone cutting suggestions - and anti-Rat
CD3

Thanks to all for your suggestions for cutting cross sections of
cortical bone. I have passed them along.

Now, what is the latest on CD3 IHC in FFPE rat tissues. I saw Ray
Koelling's suggestion in the archives using an Ab from BD Biosciences.
Is it cat # 550295?

What others if any do you suggest? HEIR ? protocol tips?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_connolly@merck.com

 
Notice:  This e-mail message, together with any attachments, contains
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please notify us immediately by reply e-mail and then delete it from 
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 4
Date: Tue, 11 May 2010 14:55:20 -0400
From: Brandi Higgins 
Subject: [Histonet] CYP.07680 - cytology cross contamination
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

Hello all,

For CAP policy CYP.07680 for "procedures to prevent cross-contamination of
specimens during processing and staining" - what are your labs doing?

 I work in a small lab so the histotechs process the cytology specimens and
the pathologists read the slides (we have no PA's or cytoprep techs or
cytotechs to screen slides).  We also process only non-GYN, so we don't have
to worry about GYN/non-GYN cross contamination.

The notes under this policy say procedures must prevent cross-contamination
between highly cellular specimens and suggest the screening method of
toluidine blue stain to determing if specimens are highly cellular.

Does anyone use the toluidine blue for this purpose?  If so could you tell
me the procedure for toluidine blue you use?  And how do you determing which
specimens you stain with toluidine blue and what qualifies as highly
cellular.  If so do you retain these toluidine blue slides for any period of
time?

CAP policy also suggests inserting a clean blank slide in each stain run and
examine for contamination.  Is anyone doing this?

We have been inspected before with no problems with this CAP question, but I
just want to make sure we are doing everything we can to prevent
cross-contamination.

Thanks in advance for your input!
Brandi Higgins, HT (ASCP)


------------------------------

Message: 5
Date: Tue, 11 May 2010 14:06:21 -0500
From: Fabrice gankam 
Subject: [Histonet] L-DNAse II vs neutrophil (leukocyte) elastase
	inhibitor
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

Hi guys
Would like to know if any of you have used the L DNAse II or the serpin B1
antibody in the rat.
very few references mentioning this antibody. Sigma has one prestige
antibody for serpin B1 but do no know if it will work on rats.
I was wondering if the DNAse II is the equivalent of the L DNAse II (product
of the degradation of serpin)
thanks for your help


------------------------------

Message: 6
Date: Tue, 11 May 2010 21:14:24 +0200
From: Toine van der Aa 
Subject: [Histonet] Genemed antibodies
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	

	
Content-Type: text/plain; charset=us-ascii

Hi,

Does anybody have experience with Genemed Biotechnologies antibodies and/or
probes of Histosonda?


Kind regards,

A.Van der Aa

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------------------------------

Message: 7
Date: Tue, 11 May 2010 12:16:33 -0700
From: sgoebel@xbiotech.com
Subject: [Histonet] ICC
To: Histonet@lists.utsouthwestern.edu
Message-ID:
	
<20100511121633.9e2d9aa830e8449a2412eb1e4f2f067e.6c1d241588.wbe@email04.secu
reserver.net>
	
Content-Type: text/plain; charset="utf-8"


   Hello all,

   I am starting ICC and flourescence for  the first time.  I have done
   tons  of  IHC  in  the  past  with DAB and AEC   slightly confused with
the way the flourescence  works.  Do you have
   to  buy  a  secondary  that  is  already tagged, or is   system  where
it  attaches to the secondary like DAB does?    anyone have any advice or
some site where I can go to get some info on
   this.   Also,  what  do you use for positive and negative controls fo   r
ICC?

   Thanks fellow histo hotties!!

   [DEL: Sarah
   Histotechni   
   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 Suite 100

   Austin, Texas  78744
   (512)386-5107


------------------------------

Message: 8
Date: Tue, 11 May 2010 16:33:12 -0400
From: "Jeter, Brent" 
Subject: RE: [Histonet] CYP.07680 - cytology cross contamination
To: Brandi Higgins ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	

	
Content-Type: text/plain; charset="us-ascii"

Hi Brandi,

The clean, blank slide is some times inserted between cases in the staining
rack to determine if floaters are present.  It's sort of a retroactive test
- if you see cells on the blank slide, then you already have
cross-contamination and you need to re-prep and restain the cases
separately.  Some people feel that you've caught the floaters on your clean
slide and the problem is solved, but there's no guarantee others won't be
present on your specimen slides, too.

Toluidine blue is an example of a stain you could use to determine
cellularity; another option often used is a modified Wright Giemsa stain
such as Diff-Quik.  You're not looking to make a final diagnosis, but to
make a quick preparation and use a simple stain just to see if there are a
lot of cells present.  This is a more proactive test.  The idea is that
highly cellular specimens have an increased likelihood of shedding cells
into the stains and contaminating other cases; it also can be used to
identify obviously malignant cases, which should definitely be stained
separately.

If you're using a rapid stain to look for high cellularity, you need to
centrifuge and concentrate the specimen(s) first, just as you would in
regular prep.  The Diff-Quik stain is a Romanovsky stain and is used on
air-dried slides, so there's no need for fixation.

Hope that helps - 


Brent Jeter
Anatomic Pathology Supervisor
The George Washington University Hospital
202-715-5076 (phone)
202-715-4691 (fax)
brent.jeter@gwu-hospital.com
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu
[histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins
[brandihiggins@gmail.com]
Sent: Tuesday, May 11, 2010 2:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CYP.07680 - cytology cross contamination

Hello all,

For CAP policy CYP.07680 for "procedures to prevent cross-contamination of
specimens during processing and staining" - what are your labs doing?

 I work in a small lab so the histotechs process the cytology specimens and
the pathologists read the slides (we have no PA's or cytoprep techs or
cytotechs to screen slides).  We also process only non-GYN, so we don't have
to worry about GYN/non-GYN cross contamination.

The notes under this policy say procedures must prevent cross-contamination
between highly cellular specimens and suggest the screening method of
toluidine blue stain to determing if specimens are highly cellular.

Does anyone use the toluidine blue for this purpose?  If so could you tell
me the procedure for toluidine blue you use?  And how do you determing which
specimens you stain with toluidine blue and what qualifies as highly
cellular.  If so do you retain these toluidine blue slides for any period of
time?

CAP policy also suggests inserting a clean blank slide in each stain run and
examine for contamination.  Is anyone doing this?

We have been inspected before with no problems with this CAP question, but I
just want to make sure we are doing everything we can to prevent
cross-contamination.

Thanks in advance for your input!
Brandi Higgins, HT (ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

UHS Confidentiality Notice:  This e-mail message, including any attachments,
is for the sole use of the intended recipient (s) and may contain
confidential and privileged information.  Any unauthorized review, use,
disclosure or distribution of this information is prohibited.  If this was
sent to you in error, please notify the sender by reply e-mail and destroy
all copies of the original message.



------------------------------

Message: 9
Date: Tue, 11 May 2010 15:53:31 -0500
From: "Steve Wong" 
Subject: [Histonet] Labomed LX400 compound microscope
To: 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Hello,

 

Does anyone have any experience with that Labomed Lx 400 compound
microscope?  It is new and it is cheap (scope with Moticam 2000 camera -
~$2,000), but I cannot find any reviews on it.

 

I'm looking for a simple microscope to take digital pictures of cross
sections of rat spinal cords for some volumetric measurements.

 

Steve

 



------------------------------

Message: 10
Date: Wed, 12 May 2010 10:53:48 +1000
From: "Tony Henwood" 
Subject: RE: [Histonet] Forwarding Request for Control Mehtod
To: "WILLIAM DESALVO" , "histonet"
	
Message-ID: 
Content-Type: text/plain; charset="us-ascii"

Ian,

What I have done in the past is to raid the Staff Fridge (or your son's
bedroom!).
There is usually old food left there that has gone moldy - bread or even
strawberries.
Wearing gloves & in a fume hood slice the bread or fruit into slices
(ensuring visible growth of fungi is present), fix for two or more days
in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod



> I wonder if you could help me find someone who would kindly donate a 
> paraffin tissue block containing candida or other fungi to be used as 
> a control block. We have had difficulty in obtaining such tissue and 
> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology departments

> are in the same position as ourselves in that the control tissue being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh lambs

> lung from a local abattoir but unfortunately the tissue seems to stain

> with PAS as well and the candida where obscured although visible 
> probably due to post mortem changes in the tissue.
 
> Alternatively have any of your members successfully produced fungal 
> control material by other means.
> 
> Thanking you in advance
 
> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland


William DeSalvo, B.S., HTL(ASCP)



 		 	   		  
_________________________________________________________________
The New Busy is not the too busy. Combine all your e-mail accounts with
Hotmail.
http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PI
D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

****************************************************************************
*****
This email and any files transmitted with it are confidential and intended
solely for the use of the individual or entity to whom they are addressed.
If you are not the intended recipient, please delete it and notify the
sender.

Views expressed in this message and any attachments are those of the
individual sender, and are not necessarily the views of The Children's
Hospital at Westmead

This note also confirms that this email message has been virus scanned and
although no computer viruses were detected, The Childrens Hospital at
Westmead accepts no liability for any consequential damage resulting from
email containing computer viruses.
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*****



------------------------------

Message: 11
Date: Tue, 11 May 2010 19:24:49 -0700 (PDT)
From: Kim Tournear 
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
To: Histonet 
Message-ID: <235254.69116.qm@web54207.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Well said Andi!!



~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
Histology Supervisor
Tucson Medical Center
Tucson,  AZ
~Don't let your life end before it begins~
OU Rocks!!!!

--- On Tue, 5/11/10, Heckford, Karen - SMMC-SF 
wrote:


From: Heckford, Karen - SMMC-SF 
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
To: "Andrea Grantham" 
Cc: histonet@lists.utsouthwestern.edu
Date: Tuesday, May 11, 2010, 8:17 AM


I am sooooo with you on this one.   HT's need to start demanding and
higher wages everywhere.  We are a rare breed with special talents.
Without a good tech you do not get good slides!!  We are not a dime a
dozen.  AUGH!  

I know just a hour north of where I am the wages can be $10-$15.00 less.
Not much difference in living expenses either.


Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea
Grantham
Sent: Tuesday, May 11, 2010 7:35 AM
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Are Histotechs considered "exempt" employees?

Anthony,
You live in CA, work 50 hour weeks and you are a HTL with an  
unprecedented and valuable resource at your disposal, Histonet and you  
know how to use it. For this you get paid $16.15/hour by what appears  
to be an employer who isn't grateful to have you. I'd start looking  
for another job.
Just my humble opinion.

Andi

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth@email.arizona.edu
Tel: 520.626.4415     Fax: 520.626.2097

"happy slicing and dicing and may all your stains work perfectly" -  
Paula Sicurello
P Please consider the environment before printing this email.




On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote:

> Hello fellow Histotechs, I have recently become certified as an HTL  
> and was wondering if anyone out there is an 'exempt' employee? I  
> live in California and feel that I am being taken advantage of.  I  
> make 16.15$ per hour and frequently work 50 hour weeks. Am I off  
> base? should I just be grateful that I have a job, as my employer so  
> frequently reminds me? Thank you Histonet! you have been an  
> invaluable resource in my career and assisting me in passing the HTL!
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      

------------------------------

Message: 12
Date: Wed, 12 May 2010 04:25:45 +0200
From: Fabrice GANKAM 
Subject: [Histonet] RE: L-DNAse II vs neutrophil (leukocyte) elastase
	inhibitor
To: 
Message-ID: <3CC647ECDCDC41E1BC06895D398ABF38@PCdeGANKAM>
Content-Type: text/plain;	charset="US-ASCII"

 

Hi guys

Would like to know if any of you have used the L DNAse II or the serpin B1
antibody in the rat.

very few references mentioning this antibody. Sigma has one prestige
antibody for serpin B1 but do no know if it will work on rats.

I was wondering if the DNAse II is the equivalent of the L DNAse II (product
of the degradation of serpin)

thanks for your help

 



------------------------------

Message: 13
Date: Wed, 12 May 2010 00:20:21 -0700
From: Emmanuel Gonz 
Subject: [Histonet] RE: Are Histotechs considered "exempt" employees?
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

>From what i know and heard, your facility is definitely getting a great
deal. It really depends on the facility. The lab i work at starts
non-certified techs around 20. I know of a small derm lab that start
non-certified techs around 18.


Emmanuel Gonzaga HT(ASCP)


------------------------------

Message: 14
Date: Wed, 12 May 2010 12:31:37 +0100
From: "Ian Montgomery" 
Subject: FW: [Histonet] Forwarding Request for Control Mehtod
To: 
Message-ID: 
Content-Type: text/plain;	charset="us-ascii"

Tony,
	Is this a universal teenage thing, filthy bedrooms? I'm convinced
there are alien life forms in my daughter's bedroom. They make the strangest
noises, grunts and groan and "I'm bored." Me, I was the perfect teenager
apart from the Celtic thing of taking to the drink and of course, lusting
after girls.
Ian. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood
Sent: 12 May 2010 01:54
To: WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Forwarding Request for Control Mehtod

Ian,

What I have done in the past is to raid the Staff Fridge (or your son's
bedroom!).
There is usually old food left there that has gone moldy - bread or even
strawberries.
Wearing gloves & in a fume hood slice the bread or fruit into slices
(ensuring visible growth of fungi is present), fix for two or more days
in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod



> I wonder if you could help me find someone who would kindly donate a 
> paraffin tissue block containing candida or other fungi to be used as 
> a control block. We have had difficulty in obtaining such tissue and 
> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology departments

> are in the same position as ourselves in that the control tissue being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh lambs

> lung from a local abattoir but unfortunately the tissue seems to stain

> with PAS as well and the candida where obscured although visible 
> probably due to post mortem changes in the tissue.
 
> Alternatively have any of your members successfully produced fungal 
> control material by other means.
> 
> Thanking you in advance
 
> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland


William DeSalvo, B.S., HTL(ASCP)



 		 	   		  
_________________________________________________________________
The New Busy is not the too busy. Combine all your e-mail accounts with
Hotmail.
http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PI
D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___________________________
____________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

****************************************************************************
*****
This email and any files transmitted with it are confidential and intended
solely for the use of the individual or entity to whom they are addressed.
If you are not the intended recipient, please delete it and notify the
sender.

Views expressed in this message and any attachments are those of the
individual sender, and are not necessarily the views of The Children's
Hospital at Westmead

This note also confirms that this email message has been virus scanned and
although no computer viruses were detected, The Childrens Hospital at
Westmead accepts no liability for any consequential damage resulting from
email containing computer viruses.
****************************************************************************
*****

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 15
Date: Wed, 12 May 2010 08:28:00 -0400
From: "Lynette Pavelich" 
Subject: Re: FW: [Histonet] Forwarding Request for Control Mehtod
To: "Ian Montgomery" ,
	
Message-ID: <4BEA6690.59CD.00EE.0@hurleymc.com>
Content-Type: text/plain; charset=US-ASCII

If you have one of those great automatic stainers that has a drain tube
going into a sink, you will also find that, after some time, a wonderful
fungus will grow inside of the tubing!! We remove the tubing and shake
the fungus loose.  Counter staining isn't the best on this type of
specimen, but when you're in a pinch, you will definitely obtain
positive fungus staining!

And, wherever you are, it must be warmer/dryer than Michigan right
now..........please send heat!!!

hope this helped!
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1@hurleymc.com
ph:  810-257-9948
Lab:  810-257-9138
fax:  810-762-7082


>>> "Ian Montgomery"  5/12/2010 7:31 AM
>>>
Tony,
	Is this a universal teenage thing, filthy bedrooms? I'm
convinced
there are alien life forms in my daughter's bedroom. They make the
strangest
noises, grunts and groan and "I'm bored." Me, I was the perfect
teenager
apart from the Celtic thing of taking to the drink and of course,
lusting
after girls.
Ian. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony
Henwood
Sent: 12 May 2010 01:54
To: WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Forwarding Request for Control Mehtod

Ian,

What I have done in the past is to raid the Staff Fridge (or your
son's
bedroom!).
There is usually old food left there that has gone moldy - bread or
even
strawberries.
Wearing gloves & in a fume hood slice the bread or fruit into slices
(ensuring visible growth of fungi is present), fix for two or more
days
in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod



> I wonder if you could help me find someone who would kindly donate a

> paraffin tissue block containing candida or other fungi to be used as

> a control block. We have had difficulty in obtaining such tissue and

> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology
departments

> are in the same position as ourselves in that the control tissue
being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh
lambs

> lung from a local abattoir but unfortunately the tissue seems to
stain

> with PAS as well and the candida where obscured although visible 
> probably due to post mortem changes in the tissue.
 
> Alternatively have any of your members successfully produced fungal 
> control material by other means.
> 
> Thanking you in advance
 
> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland


William DeSalvo, B.S., HTL(ASCP)



 		 	   		  
_________________________________________________________________
The New Busy is not the too busy. Combine all your e-mail accounts
with
Hotmail.
http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PI

D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___________________________
____________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

****************************************************************************
*****
This email and any files transmitted with it are confidential and
intended
solely for the use of the individual or entity to whom they are
addressed.
If you are not the intended recipient, please delete it and notify the
sender.

Views expressed in this message and any attachments are those of the
individual sender, and are not necessarily the views of The Children's
Hospital at Westmead

This note also confirms that this email message has been virus scanned
and
although no computer viruses were detected, The Childrens Hospital at
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 16
Date: Wed, 12 May 2010 13:34:11 +0100
From: Krish Soni 
Subject: [Histonet] Servicing of Leica equipment in the UK
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes


We are a histology lab based in the UK.  We have a Leica CV5000  
coverslipper and a Leica Autostainer XL.

We are trying to find an independent company or operator (other than  
Leica) than can service our Leica equipment for us.

Does anyone know a company or ex Leica engineer we could call?

Thanks,

Chris

m0702936@sgul.ac.uk



------------------------------

Message: 17
Date: Wed, 12 May 2010 08:42:08 -0400
From: DKBoyd@chs.net
Subject: Re: [Histonet] CYP.07680 - cytology cross contamination
To: Brandi Higgins 
Cc: histonet@lists.utsouthwestern.edu,
	histonet-bounces@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain;	charset="US-ASCII"

Brandi,
Body effusions have a high potential for cross contamination during the 
staining procedure.  Body fluids ie:  pleural fluid, peritoneal fluid, 
etc.  For that reason they are not stained with other non-gyn specimens 
ie:  bronch. wash, esoph. brush, CSF etc.  Also after the staining all 
solutions up to the 95% before the OG-6 are dumped and refilled.
The Hematoxylin, OG-6 and EA65 are filtered.  The 95% after the EA-65 is 
dumped and alcohols are rotated up.
This should prevent cross contamination.  Written documentation should be 
your procedure and proof that you are changing/filtering solutions.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd@chs.net







Brandi Higgins  
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/11/2010 03:28 PM

To
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] CYP.07680 - cytology cross contamination






Hello all,

For CAP policy CYP.07680 for "procedures to prevent cross-contamination of
specimens during processing and staining" - what are your labs doing?

 I work in a small lab so the histotechs process the cytology specimens 
and
the pathologists read the slides (we have no PA's or cytoprep techs or
cytotechs to screen slides).  We also process only non-GYN, so we don't 
have
to worry about GYN/non-GYN cross contamination.

The notes under this policy say procedures must prevent 
cross-contamination
between highly cellular specimens and suggest the screening method of
toluidine blue stain to determing if specimens are highly cellular.

Does anyone use the toluidine blue for this purpose?  If so could you tell
me the procedure for toluidine blue you use?  And how do you determing 
which
specimens you stain with toluidine blue and what qualifies as highly
cellular.  If so do you retain these toluidine blue slides for any period 
of
time?

CAP policy also suggests inserting a clean blank slide in each stain run 
and
examine for contamination.  Is anyone doing this?

We have been inspected before with no problems with this CAP question, but 
I
just want to make sure we are doing everything we can to prevent
cross-contamination.

Thanks in advance for your input!
Brandi Higgins, HT (ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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------------------------------

Message: 18
Date: Wed, 12 May 2010 09:15:18 -0400
From: bsylinda@aol.com
Subject: [Histonet] Auto Dialer for Thermo Excelsior
To: histonet@lists.utsouthwestern.edu
Message-ID: <8CCBFEC4D4344E6-C18-7D3D@webmail-d001.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"




Hello Histo,

Is there anyone out there with any experience of installing a remote voice
dialer on the processor.  We have have one and think that it has possibly
been fried due to a power surge.  However this one was purchased years ago
at Radio Shack and they no longer sell the same dialer.  The dialers that I
currently see are wireless, can anyone out there with experience with these
wireless devices give me any suggestions.

Thanks in advance,
Sylinda Battle, HT (ASCP)


------------------------------

Message: 19
Date: Wed, 12 May 2010 06:24:06 -0700 (PDT)
From: Kim Merriam 
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Message-ID: <700229.76256.qm@web50303.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Good morning,

I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a
lot of cells along the way (especially when trying to take the cells out of
the tube).  We are fixing the cells in NBF, spinning them down, adding 70%
and spinning down again.  At that point, I am trying to scoop out the cells
(with a weighing scoop) and wrap them in lens paper for processing.  I am
losing a lot of the cells during this step, because the pellet is not quite
solid.  Do you think it would be OK to let the cells air-dry a bit and then
take them out for processing?  I know this goes against everything I was
taught in histology, but I am really at a loss here.

Anyone have any hot tips for me?

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 


      

------------------------------

Message: 20
Date: Wed, 12 May 2010 06:46:28 -0700
From: sgoebel@xbiotech.com
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
To: "Kim Tournear" 
Cc: Histonet 
Message-ID:
	
<20100512064628.9e2d9aa830e8449a2412eb1e4f2f067e.af45b5d3d8.wbe@email04.secu
reserver.net>
	
Content-Type: text/plain; charset="utf-8"


   So, I had the same problem a few years ago.  They are totall   advantage
of  you.  I live in Austin where I can't imagine tha   cost  of living is
more than CA.  The average here is between 20    25 an hour, sometimes
higher if you can get a MOHS job at a priva   derm  clininc.   Start
looking, usually in my experience histo. jobs    are  everywhere,  but
sometimes  you just have to call around.  Also,
   usu   wise)  about  HT   histology department.  
   Happy hunting!!

   Sarah Goebel, B.A., HT (ASCP)

   <
   Histo   
   XBiotech USA Inc.

   8201 East Rivers
   Austin, Texas  78744
   (512)386-5107

   -------- Original Message --------
   Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
   From: Kim Tournear 
   Date: Tue, May 11, 2010 7:24 pm
   To: Histonet 
   Well said Andi!!
   ~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
   Histology Supervisor
   Tucson Medical Center
   Tucson,  AZ
   ~Don't let your life end before it begins~
   OU Rocks!!!!
   ---     On     Tue,     5/11/10,    Heckford,    Karen    -    SMMC-SF
   
   Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
   To: "Andrea Grantham" 
   Cc: histonet@lists.utsouthwestern.edu
   Date: Tuesday, May 11, 2010, 8:17 AM
   I am sooooo with you on this one.   HT's need to start deman   higher
wages everywhere.  We are a rare breed with special talents.
   Without  a  good  tech you do not get good slides!!  We are not a dime
   a<   I  know  just  a  hour north of where I am the wages can be
$10-$15.00
   less.
   Not much difference in living expenses either.
   Karen Heckford HT ASCP CE
   Lead Histology Technician
   St. Mary's Medical Center
   450 Stanyan St.
   San Francisco, Ca. 94117
   415-668-1000 ext. 6167
   -----Original Message-----
   From: histonet-bounces@lists.utsouthwestern.edu
   [[1]mailto:histonet-bounces@lists.utsouthwestern.e   Andrea
   Grantham
   Sent: Tuesday, May 11, 2010 7:35 AM
   Cc: histonet@lists.utsouthwestern.edu
   Subject: Re: [Histonet] Are Histotechs considered "exempt" employees?
   Anthony,
   You live in CA, work 50 hour weeks and you are a HTL with an
   unprecedented  and  valuable  resource  at your disposal, Histonet and
   you    know  how  to  use  it.  For  this  you  get  paid $16.15/hour by
what
   appears    to  be  an  employer who isn't grateful to have you. I'd start
looking
   <   Just my humble opinion.
   Andi
   Andrea Grantham, HT (ASCP)
   Senior Research Specialist
   University of Arizona
   Cell Biology and Anatomy
   Histology Service Laboratory
   P.O.Box 245044
   Tucson, AZ 85724
   algranth@email.arizona.edu
   Tel: 520.626.4415     Fax: 520.626.2097
   "happy  slicing  and  dicing and may all your stains work perfectly" -
   <   P Please consider the environment before printing this email.
   On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote:
   >  Hello  fellow  Histotechs,  I  have recently become certified as an
   HTL&nb   > and was wondering if anyone out there is an 'exempt' employee?
I    >  live  in California and feel that I am being taken advantage of.
I
   > make 16.15$ per hour and frequently work 50 hour weeks. Am I off    >
base?  should  I just be grateful that I have a job, as my employer
   so&n   > frequently reminds me? Thank you Histonet! you have been an
   >  invaluable  resource  in  my career and assisting me in passing the
   HTL!<   > _______________________________________________
   > Histonet mailing list
   > Histonet@lists.utsouthwestern.edu
   > [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet   >
   _______________________________________________
   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [3]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
_______________________________________________
   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
_______________________________________________
   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [5]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
References

   1. file://localhost/tmp/3D"#Compose"
   2. 3D"http://lists.utsouthwestern.edu/mailman/l   3.
3D"http://lists.utsouthwestern.edu/mailman/listin   4.
3D"http://lists.utsouthwestern.edu/mailman/listin   5.
3D"http://lists.utsouthwestern.edu/mailman/listin

------------------------------

Message: 21
Date: Wed, 12 May 2010 06:50:14 -0700 (PDT)
From: Kim Merriam 
Subject: [Histonet] FFPE cell pellet prep
To: histonet@lists.utsouthwestern.edu
Message-ID: <491802.22615.qm@web50306.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Sorry - I forgot to put a subject line.


Good morning,

I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a
lot of cells along the way (especially when trying to take the cells out of
the tube).  We are fixing the cells in NBF, spinning them down, adding 70%
and spinning down again.  At that point, I am trying to scoop out the cells
(with a weighing scoop) and wrap them in lens paper for processing.  I am
losing a lot of the cells during this step, because the pellet is not quite
solid.  Do you think it would be OK to let the cells air-dry a bit and then
take them out for processing?  I know this goes against everything I was
taught in histology, but I am really at a loss here.

Anyone have any hot tips for me?

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 



      

------------------------------

Message: 22
Date: Wed, 12 May 2010 07:02:31 -0700
From: Dawn Oakes 
Subject: [Histonet] Xylene substitutes
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	

Content-Type: text/plain; charset="us-ascii"

What are labs using for xylene substitute. I will be using it in a hand
staining setup for Mohs. Feed back on  Histo-Clear,  Clear solve, S-3
and formular 83.

Thanks in advance.

Dawn Oakes HT

 


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------------------------------

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End of Histonet Digest, Vol 78, Issue 15
****************************************



From sgoebel <@t> xbiotech.com  Wed May 12 10:21:38 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Wed May 12 10:21:49 2010
Subject: [Histonet] (no subject)
Message-ID: <20100512082138.9e2d9aa830e8449a2412eb1e4f2f067e.fbe16b6c72.wbe@email04.secureserver.net>


   I  know the histogel is pretty expensive (1500ish per kit), try ju   plain  agar  powder and mix with water...it is super cheap that way.    Happy spinning!!  =)

   Sarah Goebel, B.A., HT (ASCP)
   H   XBiotech USA Inc.
   8201 East Ri      -------- Original Message --------
   Subject: Re: [Histonet] (no subject)
   From: Kim Merriam 
   Date: Wed, May 12, 2010 7:49 am
   To: "Cormier, Kathleen" 
   Cc: Histonet , sgoebel@xbiotech.co   m
   Thanks  for  the  tips  everyone!   I  am  going  to order some aga   (histogel)  and  use  it!  We are all very excited around here abo   this, we have been pulling our hair out over these darn pellets.

   Kim Merriam, MA, HT(ASCP)QIHC
   Cambridge, MA
      <     _________________________________________________________________

   From: "Cormier, Kathleen" 

This email and any attachments have been scanned for   prior  to  leaving  the Charles River Laboratories network. Charles R   iver  Laboratories will not be liable for direct, special, indirect or
   conse   this  message  by   passed  on.   For  more    offer,  please  visit our websit   ver.com>[8]http://    

References

   1. 3D"mailto:kathleen.cormier@crl.com"
   2. 3D"mailto:histonet-bou   3. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu"
   4. 3D"mailto:histonet   5. file://localhost/tmp/3D"mail   6. 3D"http://=/
   7. 3D"http://www.criver.com"/
   8. 3D"http://www.criver.com/"
From histotech <@t> imagesbyhopper.com  Wed May 12 10:35:54 2010
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Wed May 12 10:36:08 2010
Subject: [Histonet] Are Histotechs considered "exempt"employees?
In-Reply-To: <20100512064628.9e2d9aa830e8449a2412eb1e4f2f067e.af45b5d3d8.wbe@email04.secureserver.net>
Message-ID: 

My histotechs are non-exempt employees.  In the state of FL, there is a
licensing difference between HTs and HTLs, and their allowable tasks are
different.  There is also a pay scale difference, in our facility, between
the HTs and the HTLs.  Starting wage is about $3.00 per hour different.

As the supervisor, they have made me an exempt employee.

Michelle


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
sgoebel@xbiotech.com
Sent: Wednesday, May 12, 2010 9:46 AM
To: Kim Tournear
Cc: Histonet
Subject: RE: [Histonet] Are Histotechs considered "exempt"employees?



   So, I had the same problem a few years ago.  They are totall= taking
   advantage  of  you.  I live in Austin where I can't imagine tha= the
   cost  of living is more than CA.  The average here is between 20 =nd
   25 an hour, sometimes  higher if you can get a MOHS job at a priva=e
   derm  clininc.   Start looking, usually in my experience histo. jobs
are  everywhere,  but  sometimes  you just have to call around.  Also,
   usu=ally  most  places  I've  noticed don't really seem to care (pay
   wise)  about  HT=versus  HTL  unless  you  are  wanting  to manage a
   histology department.  
   Happy hunting!!

   Sarah Goebel, B.A., HT (ASCP)

   <=iv>

   Histo=echnician
   
   XBiotech USA Inc.

   8201 East Rivers=de Dr. Bldg 4 Suite 100

   Austin, Texas  78744
   (512)386-5107

   -------- Original Message --------
   Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
   From: Kim Tournear 
   Date: Tue, May 11, 2010 7:24 pm
   To: Histonet 
   Well said Andi!!
   ~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
   Histology Supervisor
   Tucson Medical Center
   Tucson,  AZ
   ~Don't let your life end before it begins~
   OU Rocks!!!!
   ---     On     Tue,     5/11/10,    Heckford,    Karen    -    SMMC-SF
   
   Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
   To: "Andrea Grantham" 
   Cc: histonet@lists.utsouthwestern.edu
   Date: Tuesday, May 11, 2010, 8:17 AM
   I am sooooo with you on this one.   HT's need to start deman=ing and
   higher wages everywhere.  We are a rare breed with special talents.
   Without  a  good  tech you do not get good slides!!  We are not a dime
   a<=r> dozen.  AUGH!
   I  know  just  a  hour north of where I am the wages can be $10-$15.00
   less.
   Not much difference in living expenses either.
   Karen Heckford HT ASCP CE
   Lead Histology Technician
   St. Mary's Medical Center
   450 Stanyan St.
   San Francisco, Ca. 94117
   415-668-1000 ext. 6167
   -----Original Message-----
   From: histonet-bounces@lists.utsouthwestern.edu
   [[1]mailto:histonet-bounces@lists.utsouthwestern.e=du]  On Behalf Of
   Andrea
   Grantham
   Sent: Tuesday, May 11, 2010 7:35 AM
   Cc: histonet@lists.utsouthwestern.edu
   Subject: Re: [Histonet] Are Histotechs considered "exempt" employees?
   Anthony,
   You live in CA, work 50 hour weeks and you are a HTL with an
   unprecedented  and  valuable  resource  at your disposal, Histonet and
   you =
   know  how  to  use  it.  For  this  you  get  paid $16.15/hour by what
   appears    to  be  an  employer who isn't grateful to have you. I'd start
looking
   <=r> for another job.
   Just my humble opinion.
   Andi
   Andrea Grantham, HT (ASCP)
   Senior Research Specialist
   University of Arizona
   Cell Biology and Anatomy
   Histology Service Laboratory
   P.O.Box 245044
   Tucson, AZ 85724
   algranth@email.arizona.edu
   Tel: 520.626.4415     Fax: 520.626.2097
   "happy  slicing  and  dicing and may all your stains work perfectly" -
   <=r> Paula Sicurello
   P Please consider the environment before printing this email.
   On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote:
   >  Hello  fellow  Histotechs,  I  have recently become certified as an
   HTL&nb=p;
   > and was wondering if anyone out there is an 'exempt' employee? I    >
live  in California and feel that I am being taken advantage of.     I
   > make 16.15$ per hour and frequently work 50 hour weeks. Am I off    >
base?  should  I just be grateful that I have a job, as my employer
   so&n=sp;
   > frequently reminds me? Thank you Histonet! you have been an
   >  invaluable  resource  in  my career and assisting me in passing the
   HTL!<=r> >
   > _______________________________________________
   > Histonet mailing list
   > Histonet@lists.utsouthwestern.edu
   > [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet=
   >
   _______________________________________________
   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [3]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<=r>
   _______________________________________________
   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<=r>
   _______________________________________________
   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [5]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<=r>

References

   1. file://localhost/tmp/3D"#Compose"
   2. 3D"http://lists.utsouthwestern.edu/mailman/l   3.
3D"http://lists.utsouthwestern.edu/mailman/listin   4.
3D"http://lists.utsouthwestern.edu/mailman/listin   5.
3D"http://lists.utsouthwestern.edu/mailman/listin___________________________
____________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
No virus found in this incoming message.
Checked by AVG - www.avg.com 
Version: 8.5.437 / Virus Database: 271.1.1/2863 - Release Date: 05/09/10
18:26:00



From brandihiggins <@t> gmail.com  Wed May 12 10:50:27 2010
From: brandihiggins <@t> gmail.com (Brandi Higgins)
Date: Wed May 12 10:50:32 2010
Subject: [Histonet] Xylene substitutes
In-Reply-To: 
References: 
Message-ID: 

We use Formula 83 and are very happy with it.  We use it exclusively in the
VIP processor and to deparaffinize before staining.  We also use as clearing
agent after staining, prior to coverslipping, but have found that we still
need the last container before coverslipping to be xylene for best results.
Otherwise a film develops, and air bubbles are more prominent.  I tried
switching to another xylene substitute because it was slightly less
expensive, but it was not satisfactory and we went straight back to Formula
83.

Hope this helps.
Brandi Higgins, B.S. HT(ASCP)
On Wed, May 12, 2010 at 10:02 AM, Dawn Oakes wrote:

> What are labs using for xylene substitute. I will be using it in a hand
> staining setup for Mohs. Feed back on  Histo-Clear,  Clear solve, S-3
> and formular 83.
>
> Thanks in advance.
>
> Dawn Oakes HT
>
>
>
>
> -------------------------------------------------------------------------
> Confidentiality Notice:  This e-mail message, including any attachments, is
> for the sole use of the intended individual(s) named above and may contain
> confidential, privileged, and/or protected information.  Any unauthorized
> review, use, disclosure, copying, or distribution of its contents is
> prohibited.  If you are not the intended recipient, you have received this
> email in error.  If so, please notify the sender immediately by reply email
> and delete/destroy the original and all copies of this communication.  Also
> know that Internet e-mail is not secure.  In choosing to communicate with
> Olympic Medical Center by email you will assume these confidentiality risks.
>   Internet messages may become corrupted, incomplete, or may incorrectly
> identify the sender.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From TGoins <@t> mt.gov  Wed May 12 11:51:24 2010
From: TGoins <@t> mt.gov (Goins, Tresa)
Date: Wed May 12 11:51:37 2010
Subject: [Histonet] FW: AMT Cover
Message-ID: 



To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
From rosenfeldtek <@t> hotmail.com  Wed May 12 12:36:15 2010
From: rosenfeldtek <@t> hotmail.com (JR R)
Date: Wed May 12 12:36:36 2010
Subject: [Histonet] FFPE cell pellet prep
In-Reply-To: <491802.22615.qm@web50306.mail.re2.yahoo.com>
References: <491802.22615.qm@web50306.mail.re2.yahoo.com>
Message-ID: 


Here's how I do it.

I hate the lens paper method.

I transfer suspended cells from a T75 flask to a 50 ml Falcon tube and centrifuge at 400 X g for 5 minutes.  Aspirate most of the media so that cells plus media are about 1 ml.  Transfer cells plus media to a 1.7 ml microcentrifuge tube.  Pellet cells at 400 x G for 5 minutes.  Rinse with PBS if needed.  Suspend cells in NBF and fix over night.  

Next day, pellet cells and resuspend them in a small amount (100-200 microliters) of Histogel or Agar.  When the gel cools cut the microcentrifuge tube open with a scalpel or razor blade.  Now you have a nice cell pellet shaped like the bottom of your centrifuge tube, and you can treat it just like any piece of tissue. Pop it in a tissue processing cassette and then to your ethanol dehydrating steps.

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology





> Date: Wed, 12 May 2010 06:50:14 -0700
> From: kmerriam2003@yahoo.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] FFPE cell pellet prep
> 
> Sorry - I forgot to put a subject line.
> 
> 
> Good morning,
> 
> I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a lot of cells along the way (especially when trying to take the cells out of the tube).  We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again.  At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing.  I am losing a lot of the cells during this step, because the pellet is not quite solid.  Do you think it would be OK to let the cells air-dry a bit and then take them out for processing?  I know this goes against everything I was taught in histology, but I am really at a loss here.
> 
> Anyone have any hot tips for me?
> 
> Kim
>  Kim Merriam, MA, HT(ASCP)QIHC
> Cambridge, MA 
> 
> 
> 
>       
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
_________________________________________________________________
The New Busy is not the old busy. Search, chat and e-mail from your inbox.
http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3
From Margaret.Perry <@t> sdstate.edu  Wed May 12 12:42:57 2010
From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret)
Date: Wed May 12 12:43:05 2010
Subject: [Histonet] xylene substitutes
In-Reply-To: <20100512172650.348C89CBE84@barracuda.sdstate.edu>
References: <20100512172650.348C89CBE84@barracuda.sdstate.edu>
Message-ID: 


We use formula 83 for everything and do not have a problem with a film.  We have found that we must use isopropal alcohol instead of reagent alcohol in the final dehydration steps.
Margaret Perry
SDSU

From lpjones <@t> srhs-pa.org  Wed May 12 13:20:36 2010
From: lpjones <@t> srhs-pa.org (Jones, Laura)
Date: Wed May 12 13:20:42 2010
Subject: [Histonet] RE: AMT Cover
In-Reply-To: 
Message-ID: <4AE8039AEA096143B965CBC6D0921668022CA806C5@EXCH2007.srhs-pa.org>

What on earth were they thinking?!

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Goins,
Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover




To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From Wanda.Smith <@t> HCAhealthcare.com  Wed May 12 13:21:59 2010
From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com)
Date: Wed May 12 13:22:12 2010
Subject: [Histonet] RE: AMT Cover
In-Reply-To: 
References: 
Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA138AA820C6@NADCWPMSGCMS03.hca.corpad.net>

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda 


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover



To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From janderson <@t> halozyme.com  Wed May 12 14:07:51 2010
From: janderson <@t> halozyme.com (Jennifer Anderson)
Date: Wed May 12 14:08:29 2010
Subject: [Histonet] RE: AMT Cover
In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA138AA820C6@NADCWPMSGCMS03.hca.corpad.net>
References: 
	<9E2D36CE2D7CBA4A94D9B22E8328A3BA138AA820C6@NADCWPMSGCMS03.hca.corpad.net>
Message-ID: <5F7CC9B788911848A79BC83453A3D653040C50B25E@Tomlinson.hti.com>

Greetings to all.

I am SO SO happy to see that others felt the same way about the catalog cover.  I immediately went to the AMT website and gave them my opinion.  They could definitely have choreographed a more sensitive image.  I received a very nice email back, apologizing for any offense, of course un-intentional.  The advertising and media department head who sent the email assured me that they had positive feedback on the cover, in support of better techniques for cervical cancer screening.

I hope they can make better marketing decisions in the future.

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson@halozyme.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com
Sent: Wednesday, May 12, 2010 11:22 AM
To: TGoins@mt.gov; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover



To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed.  Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws.  If you received this in error, please contact the sender and delete/destroy this email.

From Terri.Brown <@t> Northside.com  Wed May 12 14:11:19 2010
From: Terri.Brown <@t> Northside.com (Terri Brown)
Date: Wed May 12 14:11:28 2010
Subject: [Histonet] RE: AMT Cover
In-Reply-To: <4AE8039AEA096143B965CBC6D0921668022CA806C5@EXCH2007.srhs-pa.org>
References: 
	<4AE8039AEA096143B965CBC6D0921668022CA806C5@EXCH2007.srhs-pa.org>
Message-ID: <731941C266951A47BEF11E5EFAAED9C90196E33F@nsmvexch01.northside.local>

How do we see the cover on line?  We did not receive a copy.

Terri H. Brown,, HT (ASCP)
Pathology Laboratory Manager
Northside Hospital  Atlanta
terri.brown@northside.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones,
Laura
Sent: Wednesday, May 12, 2010 2:21 PM
To: 'Goins, Tresa'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

What on earth were they thinking?!

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Goins,
Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover




To anyone who has received the Master Tech Flyer Volume XXXI Issue I -
please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege.


From Lynne.Bell <@t> cvmc.org  Wed May 12 14:23:59 2010
From: Lynne.Bell <@t> cvmc.org (Bell, Lynne)
Date: Wed May 12 14:24:04 2010
Subject: [Histonet] RE: AMT Cover
In-Reply-To: 
Message-ID: 

I had this catalog on my desk and everyone that came in to Histology was astounded by the cover.  No need  for the dorsal lithotomy position!

Lynne A. Bell, HT (ASCP)
Technical Specialist, Histology
Central Vermont Medical Center
130 Fisher Road
Barre, VT  05641
802-371-4923


From rachel.elliott <@t> thermofisher.com  Wed May 12 14:35:58 2010
From: rachel.elliott <@t> thermofisher.com (Elliott, Rachel A.)
Date: Wed May 12 14:36:13 2010
Subject: [Histonet] RE: AMT Cover
In-Reply-To: <5F7CC9B788911848A79BC83453A3D653040C50B25E@Tomlinson.hti.com>
References: 
	<9E2D36CE2D7CBA4A94D9B22E8328A3BA138AA820C6@NADCWPMSGCMS03.hca.corpad.net>
	<5F7CC9B788911848A79BC83453A3D653040C50B25E@Tomlinson.hti.com>
Message-ID: <7B380BF8E6354F4994F20E095D43F171EF6C7458@USPHO-MXVS01.amer.thermo.com>

So how can I see this cover?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson
Sent: Wednesday, May 12, 2010 3:08 PM
To: 'Wanda.Smith@HCAhealthcare.com'; TGoins@mt.gov; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

Greetings to all.

I am SO SO happy to see that others felt the same way about the catalog cover.  I immediately went to the AMT website and gave them my opinion.  They could definitely have choreographed a more sensitive image.  I received a very nice email back, apologizing for any offense, of course un-intentional.  The advertising and media department head who sent the email assured me that they had positive feedback on the cover, in support of better techniques for cervical cancer screening.

I hope they can make better marketing decisions in the future.

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson@halozyme.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com
Sent: Wednesday, May 12, 2010 11:22 AM
To: TGoins@mt.gov; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover



To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed.  Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws.  If you received this in error, please contact the sender and delete/destroy this email.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Montina.VanMeter <@t> pbrc.edu  Wed May 12 16:39:04 2010
From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter)
Date: Wed May 12 16:39:12 2010
Subject: [Histonet] 2010 Louisiana Society for Histotechnology State Meeting
	- Reservation Extension!
Message-ID: <4FE7FB862E90E448AE32388E759220E502507C1E@pbrcas31.pbrc.edu>

Hello Histonetters,

  The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.


 

  Meeting location:  The Embassy Suites Hotel

                                   414 Constitution Ave.

                                   Baton Rouge, LA  70808  

 

We have a block of rooms reserved for attendees at the special rate of
$99.00.  The cut-off date for reservations has been extended to May 20,
2010.   Following that date the rooms may be reserved at that rate per
availability.  The hotel will honor this rate two days prior and two
days after our meeting (please contact the Embassy Suites Hotel for
further information: 1-225-924-6566 or 1-800-Embassy).   A complimentary
hotel shuttle service is provided for those flying into Baton Rouge.
Great dining, beautiful southern plantations and gambling boats on the
Mississippi River are just a few of the attractions in the Baton Rouge
area.  Remember to ask for the Louisiana Society for Histotechnology
group when placing your reservation.  Walk-ins are always welcome!  If
you have any questions please contact:  Tina Van Meter at 225-603-0953
or vanmetmj@pbrc.edu.

 

 

Workshops:

 

 WS #1:  FISH - IT'S NOT JUST FOR DINNER!

     Bonnie Whitaker, HT (ASCP) - sponsored by Cell Marque

     OSU Medical Center

  

 WS #2:  What is the Tumor Registry?

     Cynthia Boudreaux, LPN, CTR

     Touro Infirmary

 

 WS #3:  Veterinary vs. Clinical - Which Career is Best for Me?

     Pam Marcum, B.S., M.S., HT, (ASCP) 

     UAMS

 

 WS #4:  Microtomy, It's About Technique!

     Mari Ann Mailhiot, BA, HT (ASCP)

     Leica Microsystems

 

 WS #5:  Boot Camp for Histotechs 

     Mari Ann Mailhiot, BS, HT (ASCP)

     Leica Microsystems

 

 WS #6:  So You Want to Know More About Things Your Lab Doesn't Do

    Pam Marcum, B.S., M.S., HT (ASCP),  

    UAMS

    Bonnie Whitaker, HT (ASCP) QIHC

    OSU Medical Center

          

  WS #7:  Dilution, Titrations, and Good Pipetting in the IHC Laboratory

    Robin Simpkins, HT (ASCP)    

    Biocare Medical

  

  WS #8:  Detection Chemistry & Antibody Production

    Tania Ewing-Finchem, HT (ASCP)    

    Ventana Roche

 

 

Hope to see you in June!

 

Tina

LSH Secretary

 

From histonet.nospam <@t> vneubert.com  Wed May 12 16:54:55 2010
From: histonet.nospam <@t> vneubert.com (V. Neubert)
Date: Wed May 12 16:55:09 2010
Subject: [Histonet] Xylene substitutes
In-Reply-To: 
References: 
Message-ID: <4BEB23AF.3080009@vneubert.com>

My hand staining setup contained some commercial naphta mix, which is
comparable to any other petroleum benzine solvant, for dewaxing.

For dehydrating/coverslipping, I used "n-Butyl acetate" a.k.a. "butyl
ethanoate". Coverslipping can be done with any xylene soluble glue.


> What are labs using for xylene substitute. I will be using it in a hand
> staining setup for Mohs. Feed back on  Histo-Clear,  Clear solve, S-3
> and formular 83.
>
> Thanks in advance.
>
> Dawn Oakes HT
>
>  
>
>
> -------------------------------------------------------------------------
> Confidentiality Notice:  This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information.  Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited.  If you are not the intended recipient, you have received this email in error.  If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication.  Also know that Internet e-mail is not secure.  In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks.   Internet messages may become corrupted, incomplete, or may incorrectly identify the sender.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>   


From cls71877 <@t> sbcglobal.net  Wed May 12 16:57:36 2010
From: cls71877 <@t> sbcglobal.net (Cristi stephenson)
Date: Wed May 12 16:57:43 2010
Subject: [Histonet] Gastric biopsies
Message-ID: <304545.34701.qm@web81204.mail.mud.yahoo.com>

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.? For example, does anyone run?stains to rule out H. pylori?on all gastric?biopsies?? Any information as to the standards in other labs would be great.
Thanks,
Cristi
From AnthonyH <@t> chw.edu.au  Wed May 12 17:49:31 2010
From: AnthonyH <@t> chw.edu.au (Tony Henwood)
Date: Wed May 12 17:50:00 2010
Subject: [Histonet] Forwarding Request for Control Mehtod
In-Reply-To: 
Message-ID: 

Ah yes,

I remember, actually I don't. It was a bit of a blur!!

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian
Montgomery
Sent: Wednesday, 12 May 2010 9:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: FW: [Histonet] Forwarding Request for Control Mehtod


Tony,
	Is this a universal teenage thing, filthy bedrooms? I'm
convinced there are alien life forms in my daughter's bedroom. They make
the strangest noises, grunts and groan and "I'm bored." Me, I was the
perfect teenager apart from the Celtic thing of taking to the drink and
of course, lusting after girls. Ian. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony
Henwood
Sent: 12 May 2010 01:54
To: WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Forwarding Request for Control Mehtod

Ian,

What I have done in the past is to raid the Staff Fridge (or your son's
bedroom!). There is usually old food left there that has gone moldy -
bread or even strawberries. Wearing gloves & in a fume hood slice the
bread or fruit into slices (ensuring visible growth of fungi is
present), fix for two or more days in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory
Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod



> I wonder if you could help me find someone who would kindly donate a
> paraffin tissue block containing candida or other fungi to be used as 
> a control block. We have had difficulty in obtaining such tissue and 
> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology departments

> are in the same position as ourselves in that the control tissue being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh lambs

> lung from a local abattoir but unfortunately the tissue seems to stain

> with PAS as well and the candida where obscured although visible
> probably due to post mortem changes in the tissue.
 
> Alternatively have any of your members successfully produced fungal
> control material by other means.
> 
> Thanking you in advance
 
> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland


William DeSalvo, B.S., HTL(ASCP)



 		 	   		  
_________________________________________________________________
The New Busy is not the too busy. Combine all your e-mail accounts with
Hotmail.
http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PI
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addressed. If you are not the intended recipient, please delete it and
notify the sender.

Views expressed in this message and any attachments are those of the
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From brandihiggins <@t> gmail.com  Wed May 12 18:46:32 2010
From: brandihiggins <@t> gmail.com (Brandi Higgins)
Date: Wed May 12 18:46:37 2010
Subject: [Histonet] Gastric biopsies
In-Reply-To: <304545.34701.qm@web81204.mail.mud.yahoo.com>
References: <304545.34701.qm@web81204.mail.mud.yahoo.com>
Message-ID: 

We do Diff Quik on all gastric biopsies, and we do Alcian Blue on all
esophageal biopsies with Barrett's indicated by the GI Dr.  Our other
special stains are by request, but we almost always end up doing retic and
trichrome on liver bx submitted with hepatitis as Clinical Dx.  Hope this
helps.

Brandi Higgins BS, HT(ASCP)

On Wed, May 12, 2010 at 5:57 PM, Cristi stephenson
wrote:

> Hello Histoland,
> I was wondering if anyone out there does stains other than H&E 's on
> specific tissues.  For example, does anyone run stains to rule out H.
> pylori on all gastric biopsies?  Any information as to the standards in
> other labs would be great.
> Thanks,
> Cristi
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From susanfplaza <@t> aim.com  Wed May 12 20:57:10 2010
From: susanfplaza <@t> aim.com (susanfplaza@aim.com)
Date: Wed May 12 20:57:31 2010
Subject: [Histonet] Gastric biopsies
In-Reply-To: 40009660
References: 40009660
Message-ID: <8CCC056BBCA5837-89C-3C16@webmail-d041.sysops.aol.com>


We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies.  Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori.  We occasionally did H. Pylori using immunohistochemistry.  We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies.  Hope this helps.

Susan Plaza, HT (ASCP), QIHC






-----Original Message-----
From: Cristi stephenson 
To: histonet 
Sent: Wed, May 12, 2010 5:25 pm
Subject: [Histonet] Gastric biopsies


Hello Histoland,
 was wondering if anyone out there does stains other than H&E 's on specific 
issues.  For example, does anyone run stains to rule out H. pylori on all 
astric biopsies?  Any information as to the standards in other labs would be 
reat.
hanks,
risti
______________________________________________
istonet mailing list
istonet@lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet

From macveigh <@t> usc.edu  Wed May 12 21:18:10 2010
From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni)
Date: Wed May 12 21:18:15 2010
Subject: [Histonet] How do I subscribe?
Message-ID: 

Hi all,

Can someone tell me - how does one subscribe to Master Tech Flyer?

Thank you in advance
Michelle
From dcojita <@t> tampabay.rr.com  Wed May 12 21:38:10 2010
From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com)
Date: Wed May 12 21:38:22 2010
Subject: [Histonet] job opening for cytology/ FISH and histology supervisor
	- Florida
Message-ID: 

I have a job opening for a cytotechnologist (non-gyn only) preferably with
experience with Urovysion/FISH (not required but willing to learn) in the
Tampa bay area. 

 

I also know of a job opening for a histologist; supervisory position (part
time or full time) in Ormond Beach, Florida 

 

Please call Diane anytime for details 813-843-0483

From cgill <@t> marylandgeneral.org  Thu May 13 07:05:57 2010
From: cgill <@t> marylandgeneral.org (Gill, Caula A.)
Date: Thu May 13 07:06:05 2010
Subject: [Histonet] Gastric biopsies
In-Reply-To: <304545.34701.qm@web81204.mail.mud.yahoo.com>
References: <304545.34701.qm@web81204.mail.mud.yahoo.com>
Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9E96@MDGEN-EXCH1.marylandgeneral.org>

Hello Cristi,

We routinely do a steiner on all Gastric Bx.'s we also do Pas W and WO digestion, Fe, Trichrome and retic on all liver with DX of Hepatitis. GMS-P on Lung bx POS. for HIV if requested by Dr. Elastic, retic and Trichrome  on Temporal Arteries.

Caula Gill (HT) ASCP

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson
Sent: Wednesday, May 12, 2010 5:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.? For example, does anyone run?stains to rule out H. pylori?on all gastric?biopsies?? Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Karen.Heckford <@t> CHW.edu  Thu May 13 07:09:34 2010
From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF)
Date: Thu May 13 07:10:00 2010
Subject: [Histonet] Are Histotechs considered "exempt" employees?
In-Reply-To: 
References: ,
	<2842DC75AE43AA4B92954CFB31781BC105697FA5@CHW-MSG-301.chw.edu>
	
Message-ID: <2842DC75AE43AA4B92954CFB31781BC105697FAC@CHW-MSG-301.chw.edu>

It's just crazy the wage differences around the country.  But we should
fight for the higher wages in whatever location we live in.  Not
everyone can do this type of job and be good at it.  

 

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-668-1000 ext. 6167

________________________________

From: joelle weaver [mailto:joelleweaver@hotmail.com] 
Sent: Thursday, May 13, 2010 4:24 AM
To: Heckford, Karen - SMMC-SF; histonet-bounces@lists.utsouthwestern.edu
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?

 

Boy, I wish! I live in Ohio with over 10 years bench experience,
supervisory experience, HTL and almost a masters (4 classes left)- and I
don't approach the HT scale you proposed-even the minimum.
 
> Date: Tue, 11 May 2010 05:15:40 -0700
> From: Karen.Heckford@CHW.edu
> To: sandoval.1965@hotmail.com
> Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
> CC: histonet@lists.utsouthwestern.edu
> 
> $16.15 per hour. They are getting a great deal. I believe you should
> be making at least double that. What I believe is happening is that a
> lot of offices are now starting to open their own labs and they are
> trying to get us cheap. We all need to stick to our guns and not let
> them get us cheaper than we are worth. Yes, I realize that San
> Francisco is more but the starting wage for a HT's should be $25.00 to
> $30.00 an hour to start for a newly certified tech. No matter of
> location in California. You can always negotiate higher if you have
> more experience and more certifications.
> 
> Just my two cents worth,
> 
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Anthony
> Sandoval
> Sent: Monday, May 10, 2010 9:01 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Are Histotechs considered "exempt" employees?
> 
> Hello fellow Histotechs, I have recently become certified as an HTL
and
> was 
> wondering if anyone out there is an 'exempt' employee? I live in
> California 
> and feel that I am being taken advantage of. I make 16.15$ per hour
and
> 
> frequently work 50 hour weeks. Am I off base? should I just be
grateful
> that 
> I have a job, as my employer so frequently reminds me? Thank you
> Histonet! 
> you have been an invaluable resource in my career and assisting me in 
> passing the HTL! 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________

Hotmail has tools for the New Busy. Search, chat and e-mail from your
inbox. Learn more.
 

From cpyse <@t> x-celllab.com  Thu May 13 07:37:25 2010
From: cpyse <@t> x-celllab.com (Cynthia Pyse)
Date: Thu May 13 07:37:35 2010
Subject: [Histonet] Gastric biopsies
In-Reply-To: <304545.34701.qm@web81204.mail.mud.yahoo.com>
References: <304545.34701.qm@web81204.mail.mud.yahoo.com>
Message-ID: <000001caf299$0ad3a100$207ae300$@com>

Christi
We run a diff stain on all gastric bx suspected of H. pylori as routine, it
saves us time. 

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse@x-celllab.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi
stephenson
Sent: Wednesday, May 12, 2010 5:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on
specific tissues.? For example, does anyone run?stains to rule out H.
pylori?on all gastric?biopsies?? Any information as to the standards in
other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




From rjbuesa <@t> yahoo.com  Thu May 13 08:10:22 2010
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu May 13 08:10:27 2010
Subject: [Histonet] Gastric biopsies
In-Reply-To: <304545.34701.qm@web81204.mail.mud.yahoo.com>
Message-ID: <120364.96574.qm@web65704.mail.ac4.yahoo.com>

For gastric biopsies we routinely did modified Steiner for H. pylori
Ren? J.

--- On Wed, 5/12/10, Cristi stephenson  wrote:


From: Cristi stephenson 
Subject: [Histonet] Gastric biopsies
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, May 12, 2010, 5:57 PM


Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.? For example, does anyone run?stains to rule out H. pylori?on all gastric?biopsies?? Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From JWeems <@t> sjha.org  Thu May 13 08:37:44 2010
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Thu May 13 08:37:49 2010
Subject: [Histonet] Gastric biopsies
In-Reply-To: <8CCC056BBCA5837-89C-3C16@webmail-d041.sysops.aol.com>
References: 40009660 <8CCC056BBCA5837-89C-3C16@webmail-d041.sysops.aol.com>
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DE0679E@CHEXCMS10.one.ads.che.org>

For H. pylori we do a Genta, which is a modified Steiner, with Alcian blue and H & E over it. 

I've finally gotten the docs to wait to make sure they need specials on everything else, so as not to waste. J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of susanfplaza@aim.com
Sent: Wednesday, May 12, 2010 21:57
To: cls71877@sbcglobal.net; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Gastric biopsies


We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies.  Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori.  We occasionally did H. Pylori using immunohistochemistry.  We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies.  Hope this helps.

Susan Plaza, HT (ASCP), QIHC






-----Original Message-----
From: Cristi stephenson 
To: histonet 
Sent: Wed, May 12, 2010 5:25 pm
Subject: [Histonet] Gastric biopsies


Hello Histoland,
 was wondering if anyone out there does stains other than H&E 's on specific issues.  For example, does anyone run stains to rule out H. pylori on all astric biopsies?  Any information as to the standards in other labs would be reat.
hanks,
risti
______________________________________________
istonet mailing list
istonet@lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This email, including any attachments is the 
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for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please reply to the 
sender that you have received the message in 
error, then delete this message.


From Joyce.Cline <@t> wchsys.org  Thu May 13 08:44:33 2010
From: Joyce.Cline <@t> wchsys.org (Joyce Cline)
Date: Thu May 13 08:59:17 2010
Subject: [Histonet] Gastric biopsies
In-Reply-To: <304545.34701.qm@web81204.mail.mud.yahoo.com>
References: <304545.34701.qm@web81204.mail.mud.yahoo.com>
Message-ID: 

We do Iron,Trichrome, Pas with and without Diastase and Retic on liver biopsies if requested by the grossing pathologist. We do Diff-Quik on all stomach biopsies, and if requested by the pathologist an Hp immno. A tricchrome is done on colon with a diagnosis of microscopic or collagenous colitis . A pas light green is done on esophogeal biopsies for candida. Any other specials are done at the request of the pathologist.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson [cls71877@sbcglobal.net]
Sent: Wednesday, May 12, 2010 5:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.  For example, does anyone run stains to rule out H. pylori on all gastric biopsies?  Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system.

From MPaul <@t> Alltech.com  Thu May 13 08:59:18 2010
From: MPaul <@t> Alltech.com (Marquisha Paul)
Date: Thu May 13 09:02:35 2010
Subject: [Histonet] Tilapia Fish Eye
Message-ID: <421EE4FDC90A744CB1DDDDD21A1BC56E09FBB027@ERGO.Alltech-bio.com>

Dear Histonetters,
 
Does anyone out there have experience with processing whole tilapia fish eye?  We formalin fixed our specimen, dehydrated with EtOH and cleared with Citrisolv before embedding in paraffin wax.  At the microtome we encountered all kinds of chatter.  Any advice is greatly appreciated.
 
 
Thanks,
Marquisha Paul
 
From badzrosari <@t> yahoo.com  Thu May 13 09:19:10 2010
From: badzrosari <@t> yahoo.com (Bernadette del Rosario)
Date: Thu May 13 09:19:13 2010
Subject: [Histonet] (no subject)
Message-ID: <940677.31006.qm@web52106.mail.re2.yahoo.com>

The simple 1-2% methylene blue solution..(deparaffinize,wash n water,1 dip m.blue,air dry,xylene,coverslip)..or the DIFF Kit of any brand..


      
From Jennifer.Hofecker <@t> leica-microsystems.com  Thu May 13 09:42:45 2010
From: Jennifer.Hofecker <@t> leica-microsystems.com (Jennifer.Hofecker@leica-microsystems.com)
Date: Thu May 13 09:42:57 2010
Subject: [Histonet] Hofecker, Jennifer  is out of the office.
Message-ID: 


I will be out of the office starting  05/13/2010 and will not return until
05/18/2010.

I will be on vacation from Thursday, May 13 through Tuesday, May 18. If you
need immediate assistance please contact Suzanne Mahoney at 800-248-0665
x5440.


______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________

From badzrosari <@t> yahoo.com  Thu May 13 10:01:52 2010
From: badzrosari <@t> yahoo.com (Bernadette del Rosario)
Date: Thu May 13 10:02:00 2010
Subject: [Histonet] (no subject)
Message-ID: <270439.80623.qm@web52101.mail.re2.yahoo.com>

HELLO..I wonder what reference lab i can send unstained slide for nk cells marker like cd 56 etc...im n bahrain..


      
From SHayes <@t> bloomingtonhospital.org  Thu May 13 10:43:32 2010
From: SHayes <@t> bloomingtonhospital.org (Hayes, Sherry)
Date: Thu May 13 10:43:41 2010
Subject: [Histonet] Is anyone else having an issue with their paraplast?
Message-ID: <8A307C4C42FC17478A648E933A64AAA988A67DCFB5@XCHMBCL1.neutzone.bloomhealth.org>

We are having an issue with our paraplast (manufacturer McCormick). There are black flecks and what looks like a dark sand in the paraplast. The issue has continued for @8 weeks and has happened with several lot numbers. Is anyone else experiencing this issue?

Sherry S. Hayes
Laboratory Systems Support Coordinator

Bloomington Hospital
601 West Second Street
PO Box 1149
Bloomington, IN 47402
t812.353.5606
f812.353.5584
p812.334.6337

shayes@bloomingtonhospital.org 
bloomingtonhospital.org




CONFIDENTIALITY NOTICE:
This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. 
If you are not the intended recipient, you may NOT use, disclose, copy or disseminate this information.  Please contact the sender by reply e-mail immediately and destroy all copies of the original message including all attachments. Your cooperation is greatly appreciated. 

Bloomington Hospital
P.O. Box 1149
Bloomington, IN 47402
From bcdukes <@t> lexhealth.org  Thu May 13 11:31:07 2010
From: bcdukes <@t> lexhealth.org (Blake Taylor)
Date: Thu May 13 11:31:17 2010
Subject: [Histonet] Is anyone else having an issue with their paraplast?
In-Reply-To: <8A307C4C42FC17478A648E933A64AAA988A67DCFB5@XCHMBCL1.neutzone.bloomhealth.org>
Message-ID: 

Yes, we had constant trash or sand in ours for months with different lot numbers, we finally had to change companies 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Hayes,
Sherry
Sent: Thursday, May 13, 2010 11:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Is anyone else having an issue with their paraplast?


We are having an issue with our paraplast (manufacturer McCormick). There are black flecks and what looks like a dark sand in the paraplast. The issue has continued for @8 weeks and has happened with several lot numbers. Is anyone else experiencing this issue?

Sherry S. Hayes
Laboratory Systems Support Coordinator

Bloomington Hospital
601 West Second Street
PO Box 1149
Bloomington, IN 47402
t812.353.5606
f812.353.5584
p812.334.6337

shayes@bloomingtonhospital.org 
bloomingtonhospital.org




CONFIDENTIALITY NOTICE:
This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. 
If you are not the intended recipient, you may NOT use, disclose, copy or disseminate this information.  Please contact the sender by reply e-mail immediately and destroy all copies of the original message including all attachments. Your cooperation is greatly appreciated. 

Bloomington Hospital
P.O. Box 1149
Bloomington, IN 47402
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

______________________________________________________________________
PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission.

From Joyce.Cline <@t> wchsys.org  Thu May 13 11:54:39 2010
From: Joyce.Cline <@t> wchsys.org (Joyce Cline)
Date: Thu May 13 11:58:03 2010
Subject: [Histonet] Gastric biopsies
In-Reply-To: <45EBFA1E7C931E45BBA7172D42F23C9201383187@EXCH-MBX5.Fairview.org>
References: <304545.34701.qm@web81204.mail.mud.yahoo.com>,
	,
	<45EBFA1E7C931E45BBA7172D42F23C9201383187@EXCH-MBX5.Fairview.org>
Message-ID: 

Jennifer,

I  read my response and realize I made the mistake of stating on all esophageal biopsies. We only do candida
when the Endoscopy physicians list it on the paperwork. I ran out of our control for fungus so I am purchasing controls from American Master Tech. (they use superfrost plus slides which is what Ventana approves for our NexES stainer) The American Master Tech fungus controls stain well with Pas light green and GMS.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cline@wchsys.org

________________________________________
From: Schumacher, Jennifer J [JSCHUMA1@Fairview.org]
Sent: Thursday, May 13, 2010 10:38 AM
To: Joyce Cline
Subject: RE: [Histonet] Gastric biopsies

Joyce,
I am facinated by your routine PAS/light green on esophogeal biopsies.  We are talking about working this stain up in house, but have had trouble finding candida control tissue.  May I ask what you use or how you obtain your controls?  Jennifer

________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline [Joyce.Cline@wchsys.org]
Sent: Thursday, May 13, 2010 8:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Gastric biopsies

We do Iron,Trichrome, Pas with and without Diastase and Retic on liver biopsies if requested by the grossing pathologist. We do Diff-Quik on all stomach biopsies, and if requested by the pathologist an Hp immno. A tricchrome is done on colon with a diagnosis of microscopic or collagenous colitis . A pas light green is done on esophogeal biopsies for candida. Any other specials are done at the request of the pathologist.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson [cls71877@sbcglobal.net]
Sent: Wednesday, May 12, 2010 5:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.  For example, does anyone run stains to rule out H. pylori on all gastric biopsies?  Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system.

_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From lori.garcia <@t> medtronic.com  Thu May 13 11:56:01 2010
From: lori.garcia <@t> medtronic.com (Garcia, Lori, Sr. Scientist)
Date: Thu May 13 11:58:15 2010
Subject: [Histonet] Are Histotechs considered "exempt" employees?
In-Reply-To: <2842DC75AE43AA4B92954CFB31781BC105697FAC@CHW-MSG-301.chw.edu>
References: ,
	<2842DC75AE43AA4B92954CFB31781BC105697FA5@CHW-MSG-301.chw.edu>
	
	<2842DC75AE43AA4B92954CFB31781BC105697FAC@CHW-MSG-301.chw.edu>
Message-ID: <5A8A2A45BE610D459A95CD6A9102102AF8709028@STSM1BMSGM04.ent.core.medtronic.com>

It's very hard to fight for better wages when the only options are minimum wage jobs. I was in a similar position as Joelle's in Missouri and had to fight to be paid over $10/hour after going back to my old job due to a divorce. It was 5 years before my pay was increased to what it had been just 200 miles north. I went back to school, got my Master's degree, and got the heck out of there.

Of course, it is totally unacceptable to pay that amount for an HTL in CA. If I were Anthony, I would gather all this information and show it to my employer and let he/she know that at least in histology, there are more openings than good candidates, and that if they don't appreciate me I could find an employer who does.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF
Sent: Thursday, May 13, 2010 5:10 AM
To: joelle weaver
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?

It's just crazy the wage differences around the country.  But we should fight for the higher wages in whatever location we live in.  Not everyone can do this type of job and be good at it.  

 

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-668-1000 ext. 6167

________________________________

From: joelle weaver [mailto:joelleweaver@hotmail.com]
Sent: Thursday, May 13, 2010 4:24 AM
To: Heckford, Karen - SMMC-SF; histonet-bounces@lists.utsouthwestern.edu
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?

 

Boy, I wish! I live in Ohio with over 10 years bench experience, supervisory experience, HTL and almost a masters (4 classes left)- and I don't approach the HT scale you proposed-even the minimum.
 
> Date: Tue, 11 May 2010 05:15:40 -0700
> From: Karen.Heckford@CHW.edu
> To: sandoval.1965@hotmail.com
> Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
> CC: histonet@lists.utsouthwestern.edu
> 
> $16.15 per hour. They are getting a great deal. I believe you should 
> be making at least double that. What I believe is happening is that a 
> lot of offices are now starting to open their own labs and they are 
> trying to get us cheap. We all need to stick to our guns and not let 
> them get us cheaper than we are worth. Yes, I realize that San 
> Francisco is more but the starting wage for a HT's should be $25.00 to 
> $30.00 an hour to start for a newly certified tech. No matter of 
> location in California. You can always negotiate higher if you have 
> more experience and more certifications.
> 
> Just my two cents worth,
> 
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Anthony
> Sandoval
> Sent: Monday, May 10, 2010 9:01 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Are Histotechs considered "exempt" employees?
> 
> Hello fellow Histotechs, I have recently become certified as an HTL
and
> was
> wondering if anyone out there is an 'exempt' employee? I live in 
> California and feel that I am being taken advantage of. I make 16.15$ 
> per hour
and
> 
> frequently work 50 hour weeks. Am I off base? should I just be
grateful
> that
> I have a job, as my employer so frequently reminds me? Thank you 
> Histonet!
> you have been an invaluable resource in my career and assisting me in 
> passing the HTL!
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From DKBoyd <@t> chs.net  Thu May 13 12:10:30 2010
From: DKBoyd <@t> chs.net (DKBoyd@chs.net)
Date: Thu May 13 12:10:01 2010
Subject: [Histonet] Microtome PM
Message-ID: 

Who are you all using to do your PM on your microtomes?  Not necessarily 
the manufacturer, but an independent contractor.
Thanks.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd@chs.net





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From SAllen <@t> exchange.hsc.mb.ca  Thu May 13 12:19:32 2010
From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen)
Date: Thu May 13 12:20:26 2010
Subject: [Histonet] Need Prion antibody Clone 3F4
Message-ID: 

Hi,
I need help quickly. Dako, our supplier, for Prion protein clone 3F4
(mouse anti-human), that we use for PrP IHC testing, is not supplying it
anymore .
Mine has expired & I have a ?positive to test. I am in Canada, does
anyone who does this testing know of a supplier I could contact.  
Any help would be very much appreciated.
Sharon Allen
Neuropath Lab
sallen@hsc.mb.ca
-------------- next part --------------
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addressee(s) only and may contain legally privileged or confidential information.  Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited.  If you receive this transmission in error, please notify the sender immediately and return the original.

Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original.
From BSullivan <@t> shorememorial.org  Thu May 13 12:45:04 2010
From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org)
Date: Thu May 13 12:47:08 2010
Subject: [Histonet] Microtome PM
In-Reply-To: 
Message-ID: 

I'm not sure where you live but we use a company called DOLBEY-JAMISON.
Phone number is 610-495-2010 or 1-800-220-3073. They operate out of
Pennsylvania. I have found them to be thorough and very good at their job.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


                                                                           
             DKBoyd@chs.net                                                
             Sent by:                                                      
             histonet-bounces@                                          To 
             lists.utsouthwest         histonet@lists.utsouthwestern.edu   
             ern.edu                                                    cc 
                                                                           
                                                                   Subject 
             05/13/2010 01:10          [Histonet] Microtome PM             
             PM                                                            
                                                                           
                                                                           
                                                                           
                                                                           
                                                                           




Who are you all using to do your PM on your microtomes?  Not necessarily
the manufacturer, but an independent contractor.
Thanks.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd@chs.net





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From igor.deyneko <@t> gmail.com  Thu May 13 13:00:21 2010
From: igor.deyneko <@t> gmail.com (Igor Deyneko)
Date: Thu May 13 13:00:28 2010
Subject: [Histonet] IF Doublestaining
Message-ID: 

Hello Everyone!
I'm planning to try some IF co-staining with 2 antibodies, one is a
rat-anti-mouse and the other one is rabbit anti-human on a  xenograft, each
has an appropriate secondary, donkey anti rat and donkey anti-rabbit,
conjugated to 488 and 593. Can someone advise the best way to perform such a
procedure, I'm afraid the rules of sequential staining might not work due to
fluorophore instability with washes. if anyone has performed such type of
stain, i would appreciate any tips.
Thank you.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA 02139
From brian <@t> prometheushealthcare.com  Thu May 13 13:21:13 2010
From: brian <@t> prometheushealthcare.com (Brian- Prometheus)
Date: Thu May 13 13:21:35 2010
Subject: [Histonet] New Histotechnologist Openings in NJ
Message-ID: <005e01caf2c9$1233bf90$369b3eb0$@com>

 

Multiple openings with a reference lab in Teterboro, NJ.  

 

Looking for experienced technologists to work the day shift. 

 

These positions are temporary.  

 

ASCP and NY license preferred.

 

Contact me today if you may know anyone interested!

 

 

 

Brian Feldman

Principal

Prometheus Healthcare 

Office 301-693-9057

Fax 301-368-2478

brian@prometheushealthcare.com  

www.prometheushealthcare.com  

*** Stay up to date on the newest positions and healthcare trends nationwide
on Twitter!***

 http://twitter.com/PrometheusBlog

 

From srodriguez <@t> phenopath.com  Thu May 13 13:33:09 2010
From: srodriguez <@t> phenopath.com (Stephanie Rodriguez)
Date: Thu May 13 13:33:32 2010
Subject: [Histonet] Re: Histonet Digest, Vol 78, Issue 18
Message-ID: 

Hi Bernadette,

I don?t know how complicated it would be for you to send something to the
US, but we run CD56 and all sorts of other hematologic markers on a routine
basis...(we?re a reference lab, too!)

You can find information about specimen submission and the tests we have
available at our website: www.phenopath.com.

Thanks, and good luck!

Stephanie Rodriguez, HTL(ASCP), QIHC
Senior Molecular Technologist-FISH
IHC Technologist III
Phenopath Laboratories
Seattle, WA


On 5/13/10 11:14 AM, "histonet-request@lists.utsouthwestern.edu"
 wrote:

> Message: 3
> Date: Thu, 13 May 2010 08:01:52 -0700 (PDT)
> From: Bernadette del Rosario 
> Subject: [Histonet] (no subject)
> To: Histonet@lists.utsouthwestern.edu
> Message-ID: <270439.80623.qm@web52101.mail.re2.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
> 
> HELLO..I wonder what reference lab i can send unstained slide for nk cells
> marker like cd 56 etc...im n bahrain..



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intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.
From anonwums1 <@t> gmail.com  Thu May 13 13:43:57 2010
From: anonwums1 <@t> gmail.com (Adam .)
Date: Thu May 13 13:44:02 2010
Subject: [Histonet] IF Doublestaining
In-Reply-To: 
References: 
Message-ID: 

In my experience, most fluorophores are quite stable to many washes. Here is
what I would try at first:

1) Stain with rat anti-mouse and rabbit anti-human overnight at 4C in a
humidified chamber. Dilute each antibody in a single volume of your favorite
staining buffer (PBS, TBS, TBS-T). For example, if your rat antibody needs
to be diluted in 1:100 and your rabbit needs to be diluted 1:200, take an
aliquot of 200 uL of buffer and add 2 uL of the rat primary and 1 uL of the
rabbit. Add the appropriate volume (usually 50-200 uL) of that mixture to
each slide.

2) Wash the next day, and add your secondaries again to a single mixture for
1 hour at room temperature. As long as your secondaries have been highly
cross adsorbed to many species, you shouldn't have a problem.

3) Wash, counterstain, mount, enjoy.

I really don't think you need to do sequential staining for this. I've heard
anecdotal reports of two primaries or two secondaries forming immune
complexes when mixed together, but I've never really had that problem.

Good luck,
Adam

On Thu, May 13, 2010 at 1:00 PM, Igor Deyneko wrote:

> Hello Everyone!
> I'm planning to try some IF co-staining with 2 antibodies, one is a
> rat-anti-mouse and the other one is rabbit anti-human on a  xenograft, each
> has an appropriate secondary, donkey anti rat and donkey anti-rabbit,
> conjugated to 488 and 593. Can someone advise the best way to perform such
> a
> procedure, I'm afraid the rules of sequential staining might not work due
> to
> fluorophore instability with washes. if anyone has performed such type of
> stain, i would appreciate any tips.
> Thank you.
> Igor Deyneko
> Infinity Pharmaceuticals
> Cambridge, MA 02139
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From leiker <@t> buffalo.edu  Thu May 13 13:46:56 2010
From: leiker <@t> buffalo.edu (Merced M Leiker)
Date: Thu May 13 13:49:10 2010
Subject: [Histonet] IF Doublestaining
In-Reply-To: 
References: 
Message-ID: 

You could try incubating both together (primaries and then secondaries). I 
have done this before with primaries of differing isotypes. I know others 
have, too.

Regards,
Merced

--On Thursday, May 13, 2010 2:00 PM -0400 Igor Deyneko 
 wrote:

> Hello Everyone!
> I'm planning to try some IF co-staining with 2 antibodies, one is a
> rat-anti-mouse and the other one is rabbit anti-human on a  xenograft,
> each has an appropriate secondary, donkey anti rat and donkey anti-rabbit,
> conjugated to 488 and 593. Can someone advise the best way to perform
> such a procedure, I'm afraid the rules of sequential staining might not
> work due to fluorophore instability with washes. if anyone has performed
> such type of stain, i would appreciate any tips.
> Thank you.
> Igor Deyneko
> Infinity Pharmaceuticals
> Cambridge, MA 02139
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


From Erin.Martin <@t> ucsf.edu  Thu May 13 14:02:32 2010
From: Erin.Martin <@t> ucsf.edu (Martin, Erin)
Date: Thu May 13 14:02:44 2010
Subject: [Histonet] Are Histotechs considered "exempt" employees?
Message-ID: <379A927A452F3D43A3C8705F4E67905F0FBB0F94B1@EX05.net.ucsf.edu>

I agree with Karen about a decent pay scale for California - $16.15 is a ridiculous wage for California.  Although San Francisco is on the high end because of our insanely high cost of living (San Francisco even has it's own minimum wage of $9.75/hr), $16.15 seems horribly low for anywhere in the state.   Where are you living?  Maybe if you are out in the country where there's no competition for techs it's an acceptable wage but definitely not in any city.

Just my opinion, though...  PS Hi Karen - I met you a while a couple of years ago when I stopped in St. Mary's with Denise from Dako!

Erin


Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

From Bill.Tench <@t> pph.org  Thu May 13 14:02:37 2010
From: Bill.Tench <@t> pph.org (Tench, Bill)
Date: Thu May 13 14:03:41 2010
Subject: [Histonet] histogel technique
In-Reply-To: <20100513144120.253E7128865@mail1.pph.org>
References: <20100513144120.253E7128865@mail1.pph.org>
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD028630A8@MAIL1.pph.local>

Next day, pellet cells and resuspend them in a small amount (100-200 microliters) of Histogel or Agar.  When the gel cools cut the microcentrifuge tube open with a scalpel or razor blade.  Now you have a nice cell pellet shaped like the bottom of your centrifuge tube, and you can treat it just like any piece of tissue. Pop it in a tissue processing cassette and then to your ethanol dehydrating steps.

We use histogel routinely for clinical specimens.  We have found several things that improve the process:
1)after you pellet the cells and decant, use a cotton tip swab to pick up the last little droplet of fluid adjacent to the button.  It will hold together much better.
2)after you have added histogel, vortex and centrifuge to pellet. Put tube in ice bath, add a few drops of formalin, and wait a few minutes.  With minimal "prodding" the "plug" will essentially pour out of the tube.  No need to cut.

Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Thursday, May 13, 2010 7:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [BULK] Histonet Digest, Vol 78, Issue 17

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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. RE: FFPE cell pellet prep (JR R)
   2. xylene substitutes (Perry, Margaret)
   3. RE: AMT Cover (Jones, Laura)
   4. RE: AMT Cover (Wanda.Smith@HCAhealthcare.com)
   5. RE: AMT Cover (Jennifer Anderson)
   6. RE: RE: AMT Cover (Terri Brown)
   7. RE: AMT Cover (Bell, Lynne)
   8. RE: AMT Cover (Elliott, Rachel A.)
   9. 2010 Louisiana Society for Histotechnology State Meeting	-
      Reservation Extension! (Montina Van Meter)
  10. Re: Xylene substitutes (V. Neubert)
  11. Gastric biopsies (Cristi stephenson)
  12. RE: Forwarding Request for Control Mehtod (Tony Henwood)
  13. Re: Gastric biopsies (Brandi Higgins)
  14. Re: Gastric biopsies (susanfplaza@aim.com)
  15. How do I subscribe? (Michelle MacVeigh-Aloni)
  16. job opening for cytology/ FISH and histology supervisor	-
      Florida (dcojita@tampabay.rr.com)
  17. RE: Gastric biopsies (Gill, Caula A.)
  18. RE: Are Histotechs considered "exempt" employees?
      (Heckford, Karen - SMMC-SF)
  19. RE: Gastric biopsies (Cynthia Pyse)
  20. Re: Gastric biopsies (Rene J Buesa)
  21. RE: Gastric biopsies (Weems, Joyce)
  22. RE: Gastric biopsies (Joyce Cline)
  23. Tilapia Fish Eye (Marquisha Paul)


----------------------------------------------------------------------

Message: 1
Date: Wed, 12 May 2010 10:36:15 -0700
From: JR R 
Subject: RE: [Histonet] FFPE cell pellet prep
To: 
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"


Here's how I do it.

I hate the lens paper method.

I transfer suspended cells from a T75 flask to a 50 ml Falcon tube and centrifuge at 400 X g for 5 minutes.  Aspirate most of the media so that cells plus media are about 1 ml.  Transfer cells plus media to a 1.7 ml microcentrifuge tube.  Pellet cells at 400 x G for 5 minutes.  Rinse with PBS if needed.  Suspend cells in NBF and fix over night.  

Next day, pellet cells and resuspend them in a small amount (100-200 microliters) of Histogel or Agar.  When the gel cools cut the microcentrifuge tube open with a scalpel or razor blade.  Now you have a nice cell pellet shaped like the bottom of your centrifuge tube, and you can treat it just like any piece of tissue. Pop it in a tissue processing cassette and then to your ethanol dehydrating steps.

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology





> Date: Wed, 12 May 2010 06:50:14 -0700
> From: kmerriam2003@yahoo.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] FFPE cell pellet prep
> 
> Sorry - I forgot to put a subject line.
> 
> 
> Good morning,
> 
> I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a lot of cells along the way (especially when trying to take the cells out of the tube).  We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again.  At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing.  I am losing a lot of the cells during this step, because the pellet is not quite solid.  Do you think it would be OK to let the cells air-dry a bit and then take them out for processing?  I know this goes against everything I was taught in histology, but I am really at a loss here.
> 
> Anyone have any hot tips for me?
> 
> Kim
>  Kim Merriam, MA, HT(ASCP)QIHC
> Cambridge, MA 
> 
> 
> 
>       
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
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The New Busy is not the old busy. Search, chat and e-mail from your inbox.
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------------------------------

Message: 2
Date: Wed, 12 May 2010 12:42:57 -0500
From: "Perry, Margaret" 
Subject: [Histonet] xylene substitutes
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	
Content-Type: text/plain; charset="us-ascii"


We use formula 83 for everything and do not have a problem with a film.  We have found that we must use isopropal alcohol instead of reagent alcohol in the final dehydration steps.
Margaret Perry
SDSU



------------------------------

Message: 3
Date: Wed, 12 May 2010 14:20:36 -0400
From: "Jones, Laura" 
Subject: [Histonet] RE: AMT Cover
To: "'Goins, Tresa'" ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<4AE8039AEA096143B965CBC6D0921668022CA806C5@EXCH2007.srhs-pa.org>
Content-Type: text/plain; charset="us-ascii"

What on earth were they thinking?!

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Goins,
Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover




To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 4
Date: Wed, 12 May 2010 13:21:59 -0500
From: 
Subject: [Histonet] RE: AMT Cover
To: , 
Message-ID:
	<9E2D36CE2D7CBA4A94D9B22E8328A3BA138AA820C6@NADCWPMSGCMS03.hca.corpad.net>
	
Content-Type: text/plain; charset="us-ascii"

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda 


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover



To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 5
Date: Wed, 12 May 2010 12:07:51 -0700
From: Jennifer Anderson 
Subject: [Histonet] RE: AMT Cover
To: "'Wanda.Smith@HCAhealthcare.com'" ,
	"TGoins@mt.gov" , "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<5F7CC9B788911848A79BC83453A3D653040C50B25E@Tomlinson.hti.com>
Content-Type: text/plain; charset="iso-8859-1"

Greetings to all.

I am SO SO happy to see that others felt the same way about the catalog cover.  I immediately went to the AMT website and gave them my opinion.  They could definitely have choreographed a more sensitive image.  I received a very nice email back, apologizing for any offense, of course un-intentional.  The advertising and media department head who sent the email assured me that they had positive feedback on the cover, in support of better techniques for cervical cancer screening.

I hope they can make better marketing decisions in the future.

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson@halozyme.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com
Sent: Wednesday, May 12, 2010 11:22 AM
To: TGoins@mt.gov; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover



To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed.  Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws.  If you received this in error, please contact the sender and delete/destroy this email.



------------------------------

Message: 6
Date: Wed, 12 May 2010 15:11:19 -0400
From: "Terri Brown" 
Subject: RE: [Histonet] RE: AMT Cover
To: "Jones, Laura" , "Goins, Tresa"
	,	
Message-ID:
	<731941C266951A47BEF11E5EFAAED9C90196E33F@nsmvexch01.northside.local>
Content-Type: text/plain;charset="us-ascii"

How do we see the cover on line?  We did not receive a copy.

Terri H. Brown,, HT (ASCP)
Pathology Laboratory Manager
Northside Hospital  Atlanta
terri.brown@northside.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones,
Laura
Sent: Wednesday, May 12, 2010 2:21 PM
To: 'Goins, Tresa'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

What on earth were they thinking?!

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Goins,
Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover




To anyone who has received the Master Tech Flyer Volume XXXI Issue I -
please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege.




------------------------------

Message: 7
Date: Wed, 12 May 2010 15:23:59 -0400
From: "Bell, Lynne" 
Subject: [Histonet] RE: AMT Cover
To: "'Goins, Tresa'" ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	
Content-Type: text/plain; charset="us-ascii"

I had this catalog on my desk and everyone that came in to Histology was astounded by the cover.  No need  for the dorsal lithotomy position!

Lynne A. Bell, HT (ASCP)
Technical Specialist, Histology
Central Vermont Medical Center
130 Fisher Road
Barre, VT  05641
802-371-4923




------------------------------

Message: 8
Date: Wed, 12 May 2010 12:35:58 -0700
From: "Elliott, Rachel A." 
Subject: [Histonet] RE: AMT Cover
To: Jennifer Anderson ,
	"'Wanda.Smith@HCAhealthcare.com'" ,
	"TGoins@mt.gov" , "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<7B380BF8E6354F4994F20E095D43F171EF6C7458@USPHO-MXVS01.amer.thermo.com>
	
Content-Type: text/plain; charset="us-ascii"

So how can I see this cover?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson
Sent: Wednesday, May 12, 2010 3:08 PM
To: 'Wanda.Smith@HCAhealthcare.com'; TGoins@mt.gov; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

Greetings to all.

I am SO SO happy to see that others felt the same way about the catalog cover.  I immediately went to the AMT website and gave them my opinion.  They could definitely have choreographed a more sensitive image.  I received a very nice email back, apologizing for any offense, of course un-intentional.  The advertising and media department head who sent the email assured me that they had positive feedback on the cover, in support of better techniques for cervical cancer screening.

I hope they can make better marketing decisions in the future.

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson@halozyme.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com
Sent: Wednesday, May 12, 2010 11:22 AM
To: TGoins@mt.gov; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover



To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed.  Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws.  If you received this in error, please contact the sender and delete/destroy this email.

_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 9
Date: Wed, 12 May 2010 16:39:04 -0500
From: "Montina Van Meter" 
Subject: [Histonet] 2010 Louisiana Society for Histotechnology State
	Meeting	- Reservation Extension!
To: 
Message-ID:
	<4FE7FB862E90E448AE32388E759220E502507C1E@pbrcas31.pbrc.edu>
Content-Type: text/plain;	charset="us-ascii"

Hello Histonetters,

  The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.


 

  Meeting location:  The Embassy Suites Hotel

                                   414 Constitution Ave.

                                   Baton Rouge, LA  70808  

 

We have a block of rooms reserved for attendees at the special rate of
$99.00.  The cut-off date for reservations has been extended to May 20,
2010.   Following that date the rooms may be reserved at that rate per
availability.  The hotel will honor this rate two days prior and two
days after our meeting (please contact the Embassy Suites Hotel for
further information: 1-225-924-6566 or 1-800-Embassy).   A complimentary
hotel shuttle service is provided for those flying into Baton Rouge.
Great dining, beautiful southern plantations and gambling boats on the
Mississippi River are just a few of the attractions in the Baton Rouge
area.  Remember to ask for the Louisiana Society for Histotechnology
group when placing your reservation.  Walk-ins are always welcome!  If
you have any questions please contact:  Tina Van Meter at 225-603-0953
or vanmetmj@pbrc.edu.

 

 

Workshops:

 

 WS #1:  FISH - IT'S NOT JUST FOR DINNER!

     Bonnie Whitaker, HT (ASCP) - sponsored by Cell Marque

     OSU Medical Center

  

 WS #2:  What is the Tumor Registry?

     Cynthia Boudreaux, LPN, CTR

     Touro Infirmary

 

 WS #3:  Veterinary vs. Clinical - Which Career is Best for Me?

     Pam Marcum, B.S., M.S., HT, (ASCP) 

     UAMS

 

 WS #4:  Microtomy, It's About Technique!

     Mari Ann Mailhiot, BA, HT (ASCP)

     Leica Microsystems

 

 WS #5:  Boot Camp for Histotechs 

     Mari Ann Mailhiot, BS, HT (ASCP)

     Leica Microsystems

 

 WS #6:  So You Want to Know More About Things Your Lab Doesn't Do

    Pam Marcum, B.S., M.S., HT (ASCP),  

    UAMS

    Bonnie Whitaker, HT (ASCP) QIHC

    OSU Medical Center

          

  WS #7:  Dilution, Titrations, and Good Pipetting in the IHC Laboratory

    Robin Simpkins, HT (ASCP)    

    Biocare Medical

  

  WS #8:  Detection Chemistry & Antibody Production

    Tania Ewing-Finchem, HT (ASCP)    

    Ventana Roche

 

 

Hope to see you in June!

 

Tina

LSH Secretary

 



------------------------------

Message: 10
Date: Wed, 12 May 2010 23:54:55 +0200
From: "V. Neubert" 
Subject: Re: [Histonet] Xylene substitutes
To: histonet@lists.utsouthwestern.edu
Message-ID: <4BEB23AF.3080009@vneubert.com>
Content-Type: text/plain; charset=ISO-8859-1

My hand staining setup contained some commercial naphta mix, which is
comparable to any other petroleum benzine solvant, for dewaxing.

For dehydrating/coverslipping, I used "n-Butyl acetate" a.k.a. "butyl
ethanoate". Coverslipping can be done with any xylene soluble glue.


> What are labs using for xylene substitute. I will be using it in a hand
> staining setup for Mohs. Feed back on  Histo-Clear,  Clear solve, S-3
> and formular 83.
>
> Thanks in advance.
>
> Dawn Oakes HT
>
>  
>
>
> -------------------------------------------------------------------------
> Confidentiality Notice:  This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information.  Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited.  If you are not the intended recipient, you have received this email in error.  If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication.  Also know that Internet e-mail is not secure.  In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks.   Internet messages may become corrupted, incomplete, or may incorrectly identify the sender.

> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>   




------------------------------

Message: 11
Date: Wed, 12 May 2010 14:57:36 -0700 (PDT)
From: Cristi stephenson 
Subject: [Histonet] Gastric biopsies
To: histonet@lists.utsouthwestern.edu
Message-ID: <304545.34701.qm@web81204.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.? For example, does anyone run?stains to rule out H. pylori?on all gastric?biopsies?? Any information as to the standards in other labs would be great.
Thanks,
Cristi

------------------------------

Message: 12
Date: Thu, 13 May 2010 08:49:31 +1000
From: "Tony Henwood" 
Subject: RE: [Histonet] Forwarding Request for Control Mehtod
To: "Ian Montgomery" ,
	
Message-ID: 
Content-Type: text/plain;	charset="us-ascii"

Ah yes,

I remember, actually I don't. It was a bit of a blur!!

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian
Montgomery
Sent: Wednesday, 12 May 2010 9:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: FW: [Histonet] Forwarding Request for Control Mehtod


Tony,
	Is this a universal teenage thing, filthy bedrooms? I'm
convinced there are alien life forms in my daughter's bedroom. They make
the strangest noises, grunts and groan and "I'm bored." Me, I was the
perfect teenager apart from the Celtic thing of taking to the drink and
of course, lusting after girls. Ian. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony
Henwood
Sent: 12 May 2010 01:54
To: WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Forwarding Request for Control Mehtod

Ian,

What I have done in the past is to raid the Staff Fridge (or your son's
bedroom!). There is usually old food left there that has gone moldy -
bread or even strawberries. Wearing gloves & in a fume hood slice the
bread or fruit into slices (ensuring visible growth of fungi is
present), fix for two or more days in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory
Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod



> I wonder if you could help me find someone who would kindly donate a
> paraffin tissue block containing candida or other fungi to be used as 
> a control block. We have had difficulty in obtaining such tissue and 
> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology departments

> are in the same position as ourselves in that the control tissue being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh lambs

> lung from a local abattoir but unfortunately the tissue seems to stain

> with PAS as well and the candida where obscured although visible
> probably due to post mortem changes in the tissue.
 
> Alternatively have any of your members successfully produced fungal
> control material by other means.
> 
> Thanking you in advance
 
> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland


William DeSalvo, B.S., HTL(ASCP)



 		 	   		  
_________________________________________________________________
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Hotmail.
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This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they are
addressed. If you are not the intended recipient, please delete it and
notify the sender.

Views expressed in this message and any attachments are those of the
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------------------------------

Message: 13
Date: Wed, 12 May 2010 19:46:32 -0400
From: Brandi Higgins 
Subject: Re: [Histonet] Gastric biopsies
To: Cristi stephenson 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

We do Diff Quik on all gastric biopsies, and we do Alcian Blue on all
esophageal biopsies with Barrett's indicated by the GI Dr.  Our other
special stains are by request, but we almost always end up doing retic and
trichrome on liver bx submitted with hepatitis as Clinical Dx.  Hope this
helps.

Brandi Higgins BS, HT(ASCP)

On Wed, May 12, 2010 at 5:57 PM, Cristi stephenson
wrote:

> Hello Histoland,
> I was wondering if anyone out there does stains other than H&E 's on
> specific tissues.  For example, does anyone run stains to rule out H.
> pylori on all gastric biopsies?  Any information as to the standards in
> other labs would be great.
> Thanks,
> Cristi
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 14
Date: Wed, 12 May 2010 21:57:10 -0400 (EDT)
From: susanfplaza@aim.com
Subject: Re: [Histonet] Gastric biopsies
To: cls71877@sbcglobal.net, histonet@lists.utsouthwestern.edu
Message-ID: <8CCC056BBCA5837-89C-3C16@webmail-d041.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"


We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies.  Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori.  We occasionally did H. Pylori using immunohistochemistry.  We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies.  Hope this helps.

Susan Plaza, HT (ASCP), QIHC






-----Original Message-----
From: Cristi stephenson 
To: histonet 
Sent: Wed, May 12, 2010 5:25 pm
Subject: [Histonet] Gastric biopsies


Hello Histoland,
 was wondering if anyone out there does stains other than H&E 's on specific 
issues.  For example, does anyone run stains to rule out H. pylori on all 
astric biopsies?  Any information as to the standards in other labs would be 
reat.
hanks,
risti
______________________________________________
istonet mailing list
istonet@lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 15
Date: Wed, 12 May 2010 19:18:10 -0700
From: "Michelle MacVeigh-Aloni" 
Subject: [Histonet] How do I subscribe?
To: 
Message-ID: 
Content-Type: text/plain;	charset="iso-8859-1"

Hi all,

Can someone tell me - how does one subscribe to Master Tech Flyer?

Thank you in advance
Michelle

------------------------------

Message: 16
Date: Wed, 12 May 2010 22:38:10 -0400
From: 
Subject: [Histonet] job opening for cytology/ FISH and histology
	supervisor	- Florida
To: "'histonet'" 
Message-ID:
	
	
Content-Type: text/plain;	charset="us-ascii"

I have a job opening for a cytotechnologist (non-gyn only) preferably with
experience with Urovysion/FISH (not required but willing to learn) in the
Tampa bay area. 

 

I also know of a job opening for a histologist; supervisory position (part
time or full time) in Ormond Beach, Florida 

 

Please call Diane anytime for details 813-843-0483



------------------------------

Message: 17
Date: Thu, 13 May 2010 08:05:57 -0400
From: "Gill, Caula A." 
Subject: RE: [Histonet] Gastric biopsies
To: "Cristi stephenson" ,
	
Message-ID:
	<087A9911BBAFDE4B8151CB148586E2C23A9E96@MDGEN-EXCH1.marylandgeneral.org>
	
Content-Type: text/plain;	charset="iso-8859-1"

Hello Cristi,

We routinely do a steiner on all Gastric Bx.'s we also do Pas W and WO digestion, Fe, Trichrome and retic on all liver with DX of Hepatitis. GMS-P on Lung bx POS. for HIV if requested by Dr. Elastic, retic and Trichrome  on Temporal Arteries.

Caula Gill (HT) ASCP

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson
Sent: Wednesday, May 12, 2010 5:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.? For example, does anyone run?stains to rule out H. pylori?on all gastric?biopsies?? Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 18
Date: Thu, 13 May 2010 05:09:34 -0700
From: "Heckford, Karen - SMMC-SF" 
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
To: "joelle weaver" 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	<2842DC75AE43AA4B92954CFB31781BC105697FAC@CHW-MSG-301.chw.edu>
Content-Type: text/plain;	charset="US-ASCII"

It's just crazy the wage differences around the country.  But we should
fight for the higher wages in whatever location we live in.  Not
everyone can do this type of job and be good at it.  

 

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-668-1000 ext. 6167

________________________________

From: joelle weaver [mailto:joelleweaver@hotmail.com] 
Sent: Thursday, May 13, 2010 4:24 AM
To: Heckford, Karen - SMMC-SF; histonet-bounces@lists.utsouthwestern.edu
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?

 

Boy, I wish! I live in Ohio with over 10 years bench experience,
supervisory experience, HTL and almost a masters (4 classes left)- and I
don't approach the HT scale you proposed-even the minimum.
 
> Date: Tue, 11 May 2010 05:15:40 -0700
> From: Karen.Heckford@CHW.edu
> To: sandoval.1965@hotmail.com
> Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
> CC: histonet@lists.utsouthwestern.edu
> 
> $16.15 per hour. They are getting a great deal. I believe you should
> be making at least double that. What I believe is happening is that a
> lot of offices are now starting to open their own labs and they are
> trying to get us cheap. We all need to stick to our guns and not let
> them get us cheaper than we are worth. Yes, I realize that San
> Francisco is more but the starting wage for a HT's should be $25.00 to
> $30.00 an hour to start for a newly certified tech. No matter of
> location in California. You can always negotiate higher if you have
> more experience and more certifications.
> 
> Just my two cents worth,
> 
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Anthony
> Sandoval
> Sent: Monday, May 10, 2010 9:01 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Are Histotechs considered "exempt" employees?
> 
> Hello fellow Histotechs, I have recently become certified as an HTL
and
> was 
> wondering if anyone out there is an 'exempt' employee? I live in
> California 
> and feel that I am being taken advantage of. I make 16.15$ per hour
and
> 
> frequently work 50 hour weeks. Am I off base? should I just be
grateful
> that 
> I have a job, as my employer so frequently reminds me? Thank you
> Histonet! 
> you have been an invaluable resource in my career and assisting me in 
> passing the HTL! 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________

Hotmail has tools for the New Busy. Search, chat and e-mail from your
inbox. Learn more.
 



------------------------------

Message: 19
Date: Thu, 13 May 2010 08:37:25 -0400
From: "Cynthia Pyse" 
Subject: RE: [Histonet] Gastric biopsies
To: "'Cristi stephenson'" ,
	
Message-ID: <000001caf299$0ad3a100$207ae300$@com>
Content-Type: text/plain;	charset="iso-8859-1"

Christi
We run a diff stain on all gastric bx suspected of H. pylori as routine, it
saves us time. 

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse@x-celllab.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi
stephenson
Sent: Wednesday, May 12, 2010 5:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on
specific tissues.? For example, does anyone run?stains to rule out H.
pylori?on all gastric?biopsies?? Any information as to the standards in
other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






------------------------------

Message: 20
Date: Thu, 13 May 2010 06:10:22 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Gastric biopsies
To: histonet@lists.utsouthwestern.edu,	Cristi stephenson
	
Message-ID: <120364.96574.qm@web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

For gastric biopsies we routinely did modified Steiner for H. pylori
Ren? J.

--- On Wed, 5/12/10, Cristi stephenson  wrote:


From: Cristi stephenson 
Subject: [Histonet] Gastric biopsies
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, May 12, 2010, 5:57 PM


Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.? For example, does anyone run?stains to rule out H. pylori?on all gastric?biopsies?? Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      

------------------------------

Message: 21
Date: Thu, 13 May 2010 09:37:44 -0400
From: "Weems, Joyce" 
Subject: RE: [Histonet] Gastric biopsies
To: "susanfplaza@aim.com" ,
	"cls71877@sbcglobal.net"	,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<92AD9B20A6C38C4587A9FEBE3A30E164015DE0679E@CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

For H. pylori we do a Genta, which is a modified Steiner, with Alcian blue and H & E over it. 

I've finally gotten the docs to wait to make sure they need specials on everything else, so as not to waste. J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of susanfplaza@aim.com
Sent: Wednesday, May 12, 2010 21:57
To: cls71877@sbcglobal.net; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Gastric biopsies


We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies.  Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori.  We occasionally did H. Pylori using immunohistochemistry.  We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies.  Hope this helps.

Susan Plaza, HT (ASCP), QIHC






-----Original Message-----
From: Cristi stephenson 
To: histonet 
Sent: Wed, May 12, 2010 5:25 pm
Subject: [Histonet] Gastric biopsies


Hello Histoland,
 was wondering if anyone out there does stains other than H&E 's on specific issues.  For example, does anyone run stains to rule out H. pylori on all astric biopsies?  Any information as to the standards in other labs would be reat.
hanks,
risti
______________________________________________
istonet mailing list
istonet@lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 22
Date: Thu, 13 May 2010 09:44:33 -0400
From: Joyce Cline 
Subject: RE: [Histonet] Gastric biopsies
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	
Content-Type: text/plain; charset="us-ascii"

We do Iron,Trichrome, Pas with and without Diastase and Retic on liver biopsies if requested by the grossing pathologist. We do Diff-Quik on all stomach biopsies, and if requested by the pathologist an Hp immno. A tricchrome is done on colon with a diagnosis of microscopic or collagenous colitis . A pas light green is done on esophogeal biopsies for candida. Any other specials are done at the request of the pathologist.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson [cls71877@sbcglobal.net]
Sent: Wednesday, May 12, 2010 5:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.  For example, does anyone run stains to rule out H. pylori on all gastric biopsies?  Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system.



------------------------------

Message: 23
Date: Thu, 13 May 2010 09:59:18 -0400
From: "Marquisha Paul" 
Subject: [Histonet] Tilapia Fish Eye
To: 
Message-ID:
	<421EE4FDC90A744CB1DDDDD21A1BC56E09FBB027@ERGO.Alltech-bio.com>
Content-Type: text/plain;	charset="iso-8859-1"

Dear Histonetters,
 
Does anyone out there have experience with processing whole tilapia fish eye?  We formalin fixed our specimen, dehydrated with EtOH and cleared with Citrisolv before embedding in paraffin wax.  At the microtome we encountered all kinds of chatter.  Any advice is greatly appreciated.
 
 
Thanks,
Marquisha Paul
 


------------------------------

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End of Histonet Digest, Vol 78, Issue 17
****************************************

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From Bill.Tench <@t> pph.org  Thu May 13 14:17:23 2010
From: Bill.Tench <@t> pph.org (Tench, Bill)
Date: Thu May 13 14:17:54 2010
Subject: [Histonet] routine specials
In-Reply-To: <20100513144120.253E7128865@mail1.pph.org>
References: <20100513144120.253E7128865@mail1.pph.org>
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD028630A9@MAIL1.pph.local>

Grumpy old pathologist trying to pay attention to costs:

Livers:  if the biopsy was for anything other than metastatic ca, then retic, trichrome, and iron are pretty much standard. If for met ca, you are wasting money.

Gastrics for h pylori:  Just because the GI guys asked for it doesn't mean there is any chance it is there.  In my practice, the pathologist MUST request the stain, and only if there is histologic evidence to support the dx (or the GI really whines).  We did an internal study and found in 6 months ZERO (0) cases that were positive when there was no supporting histologic change. (we do immunos)  In 95% of the cases (or more) the bugs are easily identified on H and E (you just gotta look)

Bone Marrows:  doing an iron is pretty much standard. Smear is best, at least in my lab, because it seems to go away in blocks.

Esophagus bx:  lots of folks want alcian blue for Barrett's.  Again, more than 95% of the time, the dx is obvious and the special is superfluous.

Gastric bx:  same issue with alcian blue.  It is purported to increase the sensitivity for intestinal metaplasia.  I find it hard to believe that a competent pathologist cannot interpret IM without a special stain in more than 98% (or greater) of the cases.

Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Thursday, May 13, 2010 7:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [BULK] Histonet Digest, Vol 78, Issue 17

Send Histonet mailing list submissions to
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When replying, please edit your Subject line so it is more specific
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Today's Topics:

   1. RE: FFPE cell pellet prep (JR R)
   2. xylene substitutes (Perry, Margaret)
   3. RE: AMT Cover (Jones, Laura)
   4. RE: AMT Cover (Wanda.Smith@HCAhealthcare.com)
   5. RE: AMT Cover (Jennifer Anderson)
   6. RE: RE: AMT Cover (Terri Brown)
   7. RE: AMT Cover (Bell, Lynne)
   8. RE: AMT Cover (Elliott, Rachel A.)
   9. 2010 Louisiana Society for Histotechnology State Meeting	-
      Reservation Extension! (Montina Van Meter)
  10. Re: Xylene substitutes (V. Neubert)
  11. Gastric biopsies (Cristi stephenson)
  12. RE: Forwarding Request for Control Mehtod (Tony Henwood)
  13. Re: Gastric biopsies (Brandi Higgins)
  14. Re: Gastric biopsies (susanfplaza@aim.com)
  15. How do I subscribe? (Michelle MacVeigh-Aloni)
  16. job opening for cytology/ FISH and histology supervisor	-
      Florida (dcojita@tampabay.rr.com)
  17. RE: Gastric biopsies (Gill, Caula A.)
  18. RE: Are Histotechs considered "exempt" employees?
      (Heckford, Karen - SMMC-SF)
  19. RE: Gastric biopsies (Cynthia Pyse)
  20. Re: Gastric biopsies (Rene J Buesa)
  21. RE: Gastric biopsies (Weems, Joyce)
  22. RE: Gastric biopsies (Joyce Cline)
  23. Tilapia Fish Eye (Marquisha Paul)


----------------------------------------------------------------------

Message: 1
Date: Wed, 12 May 2010 10:36:15 -0700
From: JR R 
Subject: RE: [Histonet] FFPE cell pellet prep
To: 
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"


Here's how I do it.

I hate the lens paper method.

I transfer suspended cells from a T75 flask to a 50 ml Falcon tube and centrifuge at 400 X g for 5 minutes.  Aspirate most of the media so that cells plus media are about 1 ml.  Transfer cells plus media to a 1.7 ml microcentrifuge tube.  Pellet cells at 400 x G for 5 minutes.  Rinse with PBS if needed.  Suspend cells in NBF and fix over night.  

Next day, pellet cells and resuspend them in a small amount (100-200 microliters) of Histogel or Agar.  When the gel cools cut the microcentrifuge tube open with a scalpel or razor blade.  Now you have a nice cell pellet shaped like the bottom of your centrifuge tube, and you can treat it just like any piece of tissue. Pop it in a tissue processing cassette and then to your ethanol dehydrating steps.

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology





> Date: Wed, 12 May 2010 06:50:14 -0700
> From: kmerriam2003@yahoo.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] FFPE cell pellet prep
> 
> Sorry - I forgot to put a subject line.
> 
> 
> Good morning,
> 
> I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a lot of cells along the way (especially when trying to take the cells out of the tube).  We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again.  At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing.  I am losing a lot of the cells during this step, because the pellet is not quite solid.  Do you think it would be OK to let the cells air-dry a bit and then take them out for processing?  I know this goes against everything I was taught in histology, but I am really at a loss here.
> 
> Anyone have any hot tips for me?
> 
> Kim
>  Kim Merriam, MA, HT(ASCP)QIHC
> Cambridge, MA 
> 
> 
> 
>       
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
_________________________________________________________________
The New Busy is not the old busy. Search, chat and e-mail from your inbox.
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------------------------------

Message: 2
Date: Wed, 12 May 2010 12:42:57 -0500
From: "Perry, Margaret" 
Subject: [Histonet] xylene substitutes
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	
Content-Type: text/plain; charset="us-ascii"


We use formula 83 for everything and do not have a problem with a film.  We have found that we must use isopropal alcohol instead of reagent alcohol in the final dehydration steps.
Margaret Perry
SDSU



------------------------------

Message: 3
Date: Wed, 12 May 2010 14:20:36 -0400
From: "Jones, Laura" 
Subject: [Histonet] RE: AMT Cover
To: "'Goins, Tresa'" ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<4AE8039AEA096143B965CBC6D0921668022CA806C5@EXCH2007.srhs-pa.org>
Content-Type: text/plain; charset="us-ascii"

What on earth were they thinking?!

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Goins,
Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover




To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 4
Date: Wed, 12 May 2010 13:21:59 -0500
From: 
Subject: [Histonet] RE: AMT Cover
To: , 
Message-ID:
	<9E2D36CE2D7CBA4A94D9B22E8328A3BA138AA820C6@NADCWPMSGCMS03.hca.corpad.net>
	
Content-Type: text/plain; charset="us-ascii"

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda 


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover



To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 5
Date: Wed, 12 May 2010 12:07:51 -0700
From: Jennifer Anderson 
Subject: [Histonet] RE: AMT Cover
To: "'Wanda.Smith@HCAhealthcare.com'" ,
	"TGoins@mt.gov" , "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<5F7CC9B788911848A79BC83453A3D653040C50B25E@Tomlinson.hti.com>
Content-Type: text/plain; charset="iso-8859-1"

Greetings to all.

I am SO SO happy to see that others felt the same way about the catalog cover.  I immediately went to the AMT website and gave them my opinion.  They could definitely have choreographed a more sensitive image.  I received a very nice email back, apologizing for any offense, of course un-intentional.  The advertising and media department head who sent the email assured me that they had positive feedback on the cover, in support of better techniques for cervical cancer screening.

I hope they can make better marketing decisions in the future.

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson@halozyme.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com
Sent: Wednesday, May 12, 2010 11:22 AM
To: TGoins@mt.gov; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover



To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed.  Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws.  If you received this in error, please contact the sender and delete/destroy this email.



------------------------------

Message: 6
Date: Wed, 12 May 2010 15:11:19 -0400
From: "Terri Brown" 
Subject: RE: [Histonet] RE: AMT Cover
To: "Jones, Laura" , "Goins, Tresa"
	,	
Message-ID:
	<731941C266951A47BEF11E5EFAAED9C90196E33F@nsmvexch01.northside.local>
Content-Type: text/plain;charset="us-ascii"

How do we see the cover on line?  We did not receive a copy.

Terri H. Brown,, HT (ASCP)
Pathology Laboratory Manager
Northside Hospital  Atlanta
terri.brown@northside.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones,
Laura
Sent: Wednesday, May 12, 2010 2:21 PM
To: 'Goins, Tresa'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

What on earth were they thinking?!

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Goins,
Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover




To anyone who has received the Master Tech Flyer Volume XXXI Issue I -
please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege.




------------------------------

Message: 7
Date: Wed, 12 May 2010 15:23:59 -0400
From: "Bell, Lynne" 
Subject: [Histonet] RE: AMT Cover
To: "'Goins, Tresa'" ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	
Content-Type: text/plain; charset="us-ascii"

I had this catalog on my desk and everyone that came in to Histology was astounded by the cover.  No need  for the dorsal lithotomy position!

Lynne A. Bell, HT (ASCP)
Technical Specialist, Histology
Central Vermont Medical Center
130 Fisher Road
Barre, VT  05641
802-371-4923




------------------------------

Message: 8
Date: Wed, 12 May 2010 12:35:58 -0700
From: "Elliott, Rachel A." 
Subject: [Histonet] RE: AMT Cover
To: Jennifer Anderson ,
	"'Wanda.Smith@HCAhealthcare.com'" ,
	"TGoins@mt.gov" , "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<7B380BF8E6354F4994F20E095D43F171EF6C7458@USPHO-MXVS01.amer.thermo.com>
	
Content-Type: text/plain; charset="us-ascii"

So how can I see this cover?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson
Sent: Wednesday, May 12, 2010 3:08 PM
To: 'Wanda.Smith@HCAhealthcare.com'; TGoins@mt.gov; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

Greetings to all.

I am SO SO happy to see that others felt the same way about the catalog cover.  I immediately went to the AMT website and gave them my opinion.  They could definitely have choreographed a more sensitive image.  I received a very nice email back, apologizing for any offense, of course un-intentional.  The advertising and media department head who sent the email assured me that they had positive feedback on the cover, in support of better techniques for cervical cancer screening.

I hope they can make better marketing decisions in the future.

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson@halozyme.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com
Sent: Wednesday, May 12, 2010 11:22 AM
To: TGoins@mt.gov; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover



To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed.  Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws.  If you received this in error, please contact the sender and delete/destroy this email.

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------------------------------

Message: 9
Date: Wed, 12 May 2010 16:39:04 -0500
From: "Montina Van Meter" 
Subject: [Histonet] 2010 Louisiana Society for Histotechnology State
	Meeting	- Reservation Extension!
To: 
Message-ID:
	<4FE7FB862E90E448AE32388E759220E502507C1E@pbrcas31.pbrc.edu>
Content-Type: text/plain;	charset="us-ascii"

Hello Histonetters,

  The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.


 

  Meeting location:  The Embassy Suites Hotel

                                   414 Constitution Ave.

                                   Baton Rouge, LA  70808  

 

We have a block of rooms reserved for attendees at the special rate of
$99.00.  The cut-off date for reservations has been extended to May 20,
2010.   Following that date the rooms may be reserved at that rate per
availability.  The hotel will honor this rate two days prior and two
days after our meeting (please contact the Embassy Suites Hotel for
further information: 1-225-924-6566 or 1-800-Embassy).   A complimentary
hotel shuttle service is provided for those flying into Baton Rouge.
Great dining, beautiful southern plantations and gambling boats on the
Mississippi River are just a few of the attractions in the Baton Rouge
area.  Remember to ask for the Louisiana Society for Histotechnology
group when placing your reservation.  Walk-ins are always welcome!  If
you have any questions please contact:  Tina Van Meter at 225-603-0953
or vanmetmj@pbrc.edu.

 

 

Workshops:

 

 WS #1:  FISH - IT'S NOT JUST FOR DINNER!

     Bonnie Whitaker, HT (ASCP) - sponsored by Cell Marque

     OSU Medical Center

  

 WS #2:  What is the Tumor Registry?

     Cynthia Boudreaux, LPN, CTR

     Touro Infirmary

 

 WS #3:  Veterinary vs. Clinical - Which Career is Best for Me?

     Pam Marcum, B.S., M.S., HT, (ASCP) 

     UAMS

 

 WS #4:  Microtomy, It's About Technique!

     Mari Ann Mailhiot, BA, HT (ASCP)

     Leica Microsystems

 

 WS #5:  Boot Camp for Histotechs 

     Mari Ann Mailhiot, BS, HT (ASCP)

     Leica Microsystems

 

 WS #6:  So You Want to Know More About Things Your Lab Doesn't Do

    Pam Marcum, B.S., M.S., HT (ASCP),  

    UAMS

    Bonnie Whitaker, HT (ASCP) QIHC

    OSU Medical Center

          

  WS #7:  Dilution, Titrations, and Good Pipetting in the IHC Laboratory

    Robin Simpkins, HT (ASCP)    

    Biocare Medical

  

  WS #8:  Detection Chemistry & Antibody Production

    Tania Ewing-Finchem, HT (ASCP)    

    Ventana Roche

 

 

Hope to see you in June!

 

Tina

LSH Secretary

 



------------------------------

Message: 10
Date: Wed, 12 May 2010 23:54:55 +0200
From: "V. Neubert" 
Subject: Re: [Histonet] Xylene substitutes
To: histonet@lists.utsouthwestern.edu
Message-ID: <4BEB23AF.3080009@vneubert.com>
Content-Type: text/plain; charset=ISO-8859-1

My hand staining setup contained some commercial naphta mix, which is
comparable to any other petroleum benzine solvant, for dewaxing.

For dehydrating/coverslipping, I used "n-Butyl acetate" a.k.a. "butyl
ethanoate". Coverslipping can be done with any xylene soluble glue.


> What are labs using for xylene substitute. I will be using it in a hand
> staining setup for Mohs. Feed back on  Histo-Clear,  Clear solve, S-3
> and formular 83.
>
> Thanks in advance.
>
> Dawn Oakes HT
>
>  
>
>
> -------------------------------------------------------------------------
> Confidentiality Notice:  This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information.  Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited.  If you are not the intended recipient, you have received this email in error.  If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication.  Also know that Internet e-mail is not secure.  In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks.   Internet messages may become corrupted, incomplete, or may incorrectly identify the sender.

> _______________________________________________
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>   




------------------------------

Message: 11
Date: Wed, 12 May 2010 14:57:36 -0700 (PDT)
From: Cristi stephenson 
Subject: [Histonet] Gastric biopsies
To: histonet@lists.utsouthwestern.edu
Message-ID: <304545.34701.qm@web81204.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.? For example, does anyone run?stains to rule out H. pylori?on all gastric?biopsies?? Any information as to the standards in other labs would be great.
Thanks,
Cristi

------------------------------

Message: 12
Date: Thu, 13 May 2010 08:49:31 +1000
From: "Tony Henwood" 
Subject: RE: [Histonet] Forwarding Request for Control Mehtod
To: "Ian Montgomery" ,
	
Message-ID: 
Content-Type: text/plain;	charset="us-ascii"

Ah yes,

I remember, actually I don't. It was a bit of a blur!!

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian
Montgomery
Sent: Wednesday, 12 May 2010 9:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: FW: [Histonet] Forwarding Request for Control Mehtod


Tony,
	Is this a universal teenage thing, filthy bedrooms? I'm
convinced there are alien life forms in my daughter's bedroom. They make
the strangest noises, grunts and groan and "I'm bored." Me, I was the
perfect teenager apart from the Celtic thing of taking to the drink and
of course, lusting after girls. Ian. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony
Henwood
Sent: 12 May 2010 01:54
To: WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Forwarding Request for Control Mehtod

Ian,

What I have done in the past is to raid the Staff Fridge (or your son's
bedroom!). There is usually old food left there that has gone moldy -
bread or even strawberries. Wearing gloves & in a fume hood slice the
bread or fruit into slices (ensuring visible growth of fungi is
present), fix for two or more days in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory
Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod



> I wonder if you could help me find someone who would kindly donate a
> paraffin tissue block containing candida or other fungi to be used as 
> a control block. We have had difficulty in obtaining such tissue and 
> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology departments

> are in the same position as ourselves in that the control tissue being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh lambs

> lung from a local abattoir but unfortunately the tissue seems to stain

> with PAS as well and the candida where obscured although visible
> probably due to post mortem changes in the tissue.
 
> Alternatively have any of your members successfully produced fungal
> control material by other means.
> 
> Thanking you in advance
 
> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland


William DeSalvo, B.S., HTL(ASCP)



 		 	   		  
_________________________________________________________________
The New Busy is not the too busy. Combine all your e-mail accounts with
Hotmail.
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************************************************************************
****
*****
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intended solely for the use of the individual or entity to whom they are
addressed. If you are not the intended recipient, please delete it and
notify the sender.

Views expressed in this message and any attachments are those of the
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------------------------------

Message: 13
Date: Wed, 12 May 2010 19:46:32 -0400
From: Brandi Higgins 
Subject: Re: [Histonet] Gastric biopsies
To: Cristi stephenson 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

We do Diff Quik on all gastric biopsies, and we do Alcian Blue on all
esophageal biopsies with Barrett's indicated by the GI Dr.  Our other
special stains are by request, but we almost always end up doing retic and
trichrome on liver bx submitted with hepatitis as Clinical Dx.  Hope this
helps.

Brandi Higgins BS, HT(ASCP)

On Wed, May 12, 2010 at 5:57 PM, Cristi stephenson
wrote:

> Hello Histoland,
> I was wondering if anyone out there does stains other than H&E 's on
> specific tissues.  For example, does anyone run stains to rule out H.
> pylori on all gastric biopsies?  Any information as to the standards in
> other labs would be great.
> Thanks,
> Cristi
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 14
Date: Wed, 12 May 2010 21:57:10 -0400 (EDT)
From: susanfplaza@aim.com
Subject: Re: [Histonet] Gastric biopsies
To: cls71877@sbcglobal.net, histonet@lists.utsouthwestern.edu
Message-ID: <8CCC056BBCA5837-89C-3C16@webmail-d041.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"


We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies.  Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori.  We occasionally did H. Pylori using immunohistochemistry.  We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies.  Hope this helps.

Susan Plaza, HT (ASCP), QIHC






-----Original Message-----
From: Cristi stephenson 
To: histonet 
Sent: Wed, May 12, 2010 5:25 pm
Subject: [Histonet] Gastric biopsies


Hello Histoland,
 was wondering if anyone out there does stains other than H&E 's on specific 
issues.  For example, does anyone run stains to rule out H. pylori on all 
astric biopsies?  Any information as to the standards in other labs would be 
reat.
hanks,
risti
______________________________________________
istonet mailing list
istonet@lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 15
Date: Wed, 12 May 2010 19:18:10 -0700
From: "Michelle MacVeigh-Aloni" 
Subject: [Histonet] How do I subscribe?
To: 
Message-ID: 
Content-Type: text/plain;	charset="iso-8859-1"

Hi all,

Can someone tell me - how does one subscribe to Master Tech Flyer?

Thank you in advance
Michelle

------------------------------

Message: 16
Date: Wed, 12 May 2010 22:38:10 -0400
From: 
Subject: [Histonet] job opening for cytology/ FISH and histology
	supervisor	- Florida
To: "'histonet'" 
Message-ID:
	
	
Content-Type: text/plain;	charset="us-ascii"

I have a job opening for a cytotechnologist (non-gyn only) preferably with
experience with Urovysion/FISH (not required but willing to learn) in the
Tampa bay area. 

 

I also know of a job opening for a histologist; supervisory position (part
time or full time) in Ormond Beach, Florida 

 

Please call Diane anytime for details 813-843-0483



------------------------------

Message: 17
Date: Thu, 13 May 2010 08:05:57 -0400
From: "Gill, Caula A." 
Subject: RE: [Histonet] Gastric biopsies
To: "Cristi stephenson" ,
	
Message-ID:
	<087A9911BBAFDE4B8151CB148586E2C23A9E96@MDGEN-EXCH1.marylandgeneral.org>
	
Content-Type: text/plain;	charset="iso-8859-1"

Hello Cristi,

We routinely do a steiner on all Gastric Bx.'s we also do Pas W and WO digestion, Fe, Trichrome and retic on all liver with DX of Hepatitis. GMS-P on Lung bx POS. for HIV if requested by Dr. Elastic, retic and Trichrome  on Temporal Arteries.

Caula Gill (HT) ASCP

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson
Sent: Wednesday, May 12, 2010 5:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.? For example, does anyone run?stains to rule out H. pylori?on all gastric?biopsies?? Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 18
Date: Thu, 13 May 2010 05:09:34 -0700
From: "Heckford, Karen - SMMC-SF" 
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
To: "joelle weaver" 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	<2842DC75AE43AA4B92954CFB31781BC105697FAC@CHW-MSG-301.chw.edu>
Content-Type: text/plain;	charset="US-ASCII"

It's just crazy the wage differences around the country.  But we should
fight for the higher wages in whatever location we live in.  Not
everyone can do this type of job and be good at it.  

 

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-668-1000 ext. 6167

________________________________

From: joelle weaver [mailto:joelleweaver@hotmail.com] 
Sent: Thursday, May 13, 2010 4:24 AM
To: Heckford, Karen - SMMC-SF; histonet-bounces@lists.utsouthwestern.edu
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?

 

Boy, I wish! I live in Ohio with over 10 years bench experience,
supervisory experience, HTL and almost a masters (4 classes left)- and I
don't approach the HT scale you proposed-even the minimum.
 
> Date: Tue, 11 May 2010 05:15:40 -0700
> From: Karen.Heckford@CHW.edu
> To: sandoval.1965@hotmail.com
> Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
> CC: histonet@lists.utsouthwestern.edu
> 
> $16.15 per hour. They are getting a great deal. I believe you should
> be making at least double that. What I believe is happening is that a
> lot of offices are now starting to open their own labs and they are
> trying to get us cheap. We all need to stick to our guns and not let
> them get us cheaper than we are worth. Yes, I realize that San
> Francisco is more but the starting wage for a HT's should be $25.00 to
> $30.00 an hour to start for a newly certified tech. No matter of
> location in California. You can always negotiate higher if you have
> more experience and more certifications.
> 
> Just my two cents worth,
> 
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Anthony
> Sandoval
> Sent: Monday, May 10, 2010 9:01 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Are Histotechs considered "exempt" employees?
> 
> Hello fellow Histotechs, I have recently become certified as an HTL
and
> was 
> wondering if anyone out there is an 'exempt' employee? I live in
> California 
> and feel that I am being taken advantage of. I make 16.15$ per hour
and
> 
> frequently work 50 hour weeks. Am I off base? should I just be
grateful
> that 
> I have a job, as my employer so frequently reminds me? Thank you
> Histonet! 
> you have been an invaluable resource in my career and assisting me in 
> passing the HTL! 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________

Hotmail has tools for the New Busy. Search, chat and e-mail from your
inbox. Learn more.
 



------------------------------

Message: 19
Date: Thu, 13 May 2010 08:37:25 -0400
From: "Cynthia Pyse" 
Subject: RE: [Histonet] Gastric biopsies
To: "'Cristi stephenson'" ,
	
Message-ID: <000001caf299$0ad3a100$207ae300$@com>
Content-Type: text/plain;	charset="iso-8859-1"

Christi
We run a diff stain on all gastric bx suspected of H. pylori as routine, it
saves us time. 

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse@x-celllab.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi
stephenson
Sent: Wednesday, May 12, 2010 5:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on
specific tissues.? For example, does anyone run?stains to rule out H.
pylori?on all gastric?biopsies?? Any information as to the standards in
other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






------------------------------

Message: 20
Date: Thu, 13 May 2010 06:10:22 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Gastric biopsies
To: histonet@lists.utsouthwestern.edu,	Cristi stephenson
	
Message-ID: <120364.96574.qm@web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

For gastric biopsies we routinely did modified Steiner for H. pylori
Ren? J.

--- On Wed, 5/12/10, Cristi stephenson  wrote:


From: Cristi stephenson 
Subject: [Histonet] Gastric biopsies
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, May 12, 2010, 5:57 PM


Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.? For example, does anyone run?stains to rule out H. pylori?on all gastric?biopsies?? Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      

------------------------------

Message: 21
Date: Thu, 13 May 2010 09:37:44 -0400
From: "Weems, Joyce" 
Subject: RE: [Histonet] Gastric biopsies
To: "susanfplaza@aim.com" ,
	"cls71877@sbcglobal.net"	,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<92AD9B20A6C38C4587A9FEBE3A30E164015DE0679E@CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

For H. pylori we do a Genta, which is a modified Steiner, with Alcian blue and H & E over it. 

I've finally gotten the docs to wait to make sure they need specials on everything else, so as not to waste. J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of susanfplaza@aim.com
Sent: Wednesday, May 12, 2010 21:57
To: cls71877@sbcglobal.net; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Gastric biopsies


We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies.  Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori.  We occasionally did H. Pylori using immunohistochemistry.  We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies.  Hope this helps.

Susan Plaza, HT (ASCP), QIHC






-----Original Message-----
From: Cristi stephenson 
To: histonet 
Sent: Wed, May 12, 2010 5:25 pm
Subject: [Histonet] Gastric biopsies


Hello Histoland,
 was wondering if anyone out there does stains other than H&E 's on specific issues.  For example, does anyone run stains to rule out H. pylori on all astric biopsies?  Any information as to the standards in other labs would be reat.
hanks,
risti
______________________________________________
istonet mailing list
istonet@lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This email, including any attachments is the 
property of Catholic Health East and is intended 
for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please reply to the 
sender that you have received the message in 
error, then delete this message.




------------------------------

Message: 22
Date: Thu, 13 May 2010 09:44:33 -0400
From: Joyce Cline 
Subject: RE: [Histonet] Gastric biopsies
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	
Content-Type: text/plain; charset="us-ascii"

We do Iron,Trichrome, Pas with and without Diastase and Retic on liver biopsies if requested by the grossing pathologist. We do Diff-Quik on all stomach biopsies, and if requested by the pathologist an Hp immno. A tricchrome is done on colon with a diagnosis of microscopic or collagenous colitis . A pas light green is done on esophogeal biopsies for candida. Any other specials are done at the request of the pathologist.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson [cls71877@sbcglobal.net]
Sent: Wednesday, May 12, 2010 5:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.  For example, does anyone run stains to rule out H. pylori on all gastric biopsies?  Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system.



------------------------------

Message: 23
Date: Thu, 13 May 2010 09:59:18 -0400
From: "Marquisha Paul" 
Subject: [Histonet] Tilapia Fish Eye
To: 
Message-ID:
	<421EE4FDC90A744CB1DDDDD21A1BC56E09FBB027@ERGO.Alltech-bio.com>
Content-Type: text/plain;	charset="iso-8859-1"

Dear Histonetters,
 
Does anyone out there have experience with processing whole tilapia fish eye?  We formalin fixed our specimen, dehydrated with EtOH and cleared with Citrisolv before embedding in paraffin wax.  At the microtome we encountered all kinds of chatter.  Any advice is greatly appreciated.
 
 
Thanks,
Marquisha Paul
 


------------------------------

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End of Histonet Digest, Vol 78, Issue 17
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From SLB <@t> stowers.org  Thu May 13 14:28:39 2010
From: SLB <@t> stowers.org (Beckham, Sharon)
Date: Thu May 13 14:28:55 2010
Subject: [Histonet] IF Doublestaining
In-Reply-To: 
References: 
	
Message-ID: 

Yes, I do double staining very frequently the way  Adam stated.  I hardly ever have to do the sequential staining.
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam .
Sent: Thursday, May 13, 2010 1:44 PM
To: Igor Deyneko
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IF Doublestaining

In my experience, most fluorophores are quite stable to many washes. Here is what I would try at first:

1) Stain with rat anti-mouse and rabbit anti-human overnight at 4C in a humidified chamber. Dilute each antibody in a single volume of your favorite staining buffer (PBS, TBS, TBS-T). For example, if your rat antibody needs to be diluted in 1:100 and your rabbit needs to be diluted 1:200, take an aliquot of 200 uL of buffer and add 2 uL of the rat primary and 1 uL of the rabbit. Add the appropriate volume (usually 50-200 uL) of that mixture to each slide.

2) Wash the next day, and add your secondaries again to a single mixture for
1 hour at room temperature. As long as your secondaries have been highly cross adsorbed to many species, you shouldn't have a problem.

3) Wash, counterstain, mount, enjoy.

I really don't think you need to do sequential staining for this. I've heard anecdotal reports of two primaries or two secondaries forming immune complexes when mixed together, but I've never really had that problem.

Good luck,
Adam

On Thu, May 13, 2010 at 1:00 PM, Igor Deyneko wrote:

> Hello Everyone!
> I'm planning to try some IF co-staining with 2 antibodies, one is a 
> rat-anti-mouse and the other one is rabbit anti-human on a  xenograft, 
> each has an appropriate secondary, donkey anti rat and donkey 
> anti-rabbit, conjugated to 488 and 593. Can someone advise the best 
> way to perform such a procedure, I'm afraid the rules of sequential 
> staining might not work due to fluorophore instability with washes. if 
> anyone has performed such type of stain, i would appreciate any tips.
> Thank you.
> Igor Deyneko
> Infinity Pharmaceuticals
> Cambridge, MA 02139
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From leiker <@t> buffalo.edu  Thu May 13 14:43:53 2010
From: leiker <@t> buffalo.edu (Merced M Leiker)
Date: Thu May 13 14:44:53 2010
Subject: [Histonet] IF Doublestaining
In-Reply-To: 
References: 
	
Message-ID: <7956EB7E16561D5D4E2E60FA@CDYwxp1931.ad.med.buffalo.edu>

I'm not sure if that was clear looking back on it:

Incubate both primaries together.
Then incubate both secondaries together.


--On Thursday, May 13, 2010 2:46 PM -0400 Merced M Leiker 
 wrote:

> You could try incubating both together (primaries and then secondaries).
> I have done this before with primaries of differing isotypes. I know
> others have, too.
>
> Regards,
> Merced
>
> --On Thursday, May 13, 2010 2:00 PM -0400 Igor Deyneko
>  wrote:
>
>> Hello Everyone!
>> I'm planning to try some IF co-staining with 2 antibodies, one is a
>> rat-anti-mouse and the other one is rabbit anti-human on a  xenograft,
>> each has an appropriate secondary, donkey anti rat and donkey
>> anti-rabbit, conjugated to 488 and 593. Can someone advise the best way
>> to perform such a procedure, I'm afraid the rules of sequential staining
>> might not work due to fluorophore instability with washes. if anyone has
>> performed such type of stain, i would appreciate any tips.
>> Thank you.
>> Igor Deyneko
>> Infinity Pharmaceuticals
>> Cambridge, MA 02139
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> Merced M Leiker
> Research Technician III
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214  USA
> leiker@buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
>
> No trees were harmed in the sending of this email.
> However, many electrons were severely inconvenienced.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


From johnsonmeb <@t> qwest.net  Thu May 13 15:40:56 2010
From: johnsonmeb <@t> qwest.net (Mark E B Johnson, MD, MBA, FCAP)
Date: Thu May 13 15:41:10 2010
Subject: [Histonet] Histotech/Mohs Tech Job Opportunity In Phoenix
Message-ID: <98E6E691EBE24CBBB6C635F75E2F9A14@arizona0st8a09>

A growing dermatology/pathology practice in Phoenix seeks a part-time
certified and fully accredited

histotechnologist for its growing pathology business as well as a Mohs tech
for Mohs surgery.

 

The ideal applicant would be one person competent in both, although
individuals qualified in either field

are welcome to apply.

 

The histotechnologist position will initially be a part-time position, two
days per week, typically

Mondays and Thursdays, with a flexible time schedule expected to evolve into
a full-time employment position as the pathology business grows.

 

This position would be ideal for a certified histotech who already has a
full-time job and desires to earn extra income in a flexible environment
simultaneously establishing themselves in an opportune position for future
full-time employment with our medical practice.

 

The Mohs tech position presently requires one day per month of work with the
opportunity to work

with a nationally renowned Mohs surgeon.

 

Certified candidates with pleasant personalities and responsible work ethics
who can work in a team

environment are preferred.

 

Please email your resume with cover letter to azdermjob@qwest.net, or you
may fax it to (480) 718-

7342.

From Gina.Rodriguez <@t> leica-microsystems.com  Thu May 13 16:01:32 2010
From: Gina.Rodriguez <@t> leica-microsystems.com (Gina.Rodriguez@leica-microsystems.com)
Date: Thu May 13 16:01:39 2010
Subject: [Histonet] Gina Rodriguez is out of the office.
Message-ID: 


I will be out of the office starting  05/13/2010 and will not return until
05/17/2010.

I will respond to your message when I return.


______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________

From andreahooper <@t> rocketmail.com  Thu May 13 17:33:20 2010
From: andreahooper <@t> rocketmail.com (andreahooper@rocketmail.com)
Date: Thu May 13 17:33:33 2010
Subject: [Histonet] IF Doublestaining
In-Reply-To: 
References: 
Message-ID: <1284993741-1273790000-cardhu_decombobulator_blackberry.rim.net-363112389-@bda2667.bisx.prod.on.blackberry>

I completely agree with all Adam has said. 

The essential key is to make sure your secondaries are highly cross adsorbed to each other and including mouse since you are staining mouse stroma. Also run each as singles alongside your double - that will give you great confidence in what you are seeing is real.

Should be a piece of cake ;)



Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: "Adam ." 
Date: Thu, 13 May 2010 13:43:57 
To: Igor Deyneko
Cc: 
Subject: Re: [Histonet] IF Doublestaining

In my experience, most fluorophores are quite stable to many washes. Here is
what I would try at first:

1) Stain with rat anti-mouse and rabbit anti-human overnight at 4C in a
humidified chamber. Dilute each antibody in a single volume of your favorite
staining buffer (PBS, TBS, TBS-T). For example, if your rat antibody needs
to be diluted in 1:100 and your rabbit needs to be diluted 1:200, take an
aliquot of 200 uL of buffer and add 2 uL of the rat primary and 1 uL of the
rabbit. Add the appropriate volume (usually 50-200 uL) of that mixture to
each slide.

2) Wash the next day, and add your secondaries again to a single mixture for
1 hour at room temperature. As long as your secondaries have been highly
cross adsorbed to many species, you shouldn't have a problem.

3) Wash, counterstain, mount, enjoy.

I really don't think you need to do sequential staining for this. I've heard
anecdotal reports of two primaries or two secondaries forming immune
complexes when mixed together, but I've never really had that problem.

Good luck,
Adam

On Thu, May 13, 2010 at 1:00 PM, Igor Deyneko wrote:

> Hello Everyone!
> I'm planning to try some IF co-staining with 2 antibodies, one is a
> rat-anti-mouse and the other one is rabbit anti-human on a  xenograft, each
> has an appropriate secondary, donkey anti rat and donkey anti-rabbit,
> conjugated to 488 and 593. Can someone advise the best way to perform such
> a
> procedure, I'm afraid the rules of sequential staining might not work due
> to
> fluorophore instability with washes. if anyone has performed such type of
> stain, i would appreciate any tips.
> Thank you.
> Igor Deyneko
> Infinity Pharmaceuticals
> Cambridge, MA 02139
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From chak_bou <@t> yahoo.com  Fri May 14 06:02:29 2010
From: chak_bou <@t> yahoo.com (Chakib Boussahmain)
Date: Fri May 14 06:02:33 2010
Subject: [Histonet] Campylobacter jejuni antibody?
Message-ID: <387966.8087.qm@web58107.mail.re3.yahoo.com>

Hello histonet,
I need help with Campylobacter jejuni antibody, where can I purchase it? Protocol? Control slides?
Any help would be very much appreciated!
Thank you
Chakib
Histology supervisor HTL(ASCP)
MIT-DCM


      
From sprice2003 <@t> gmail.com  Fri May 14 06:49:51 2010
From: sprice2003 <@t> gmail.com (Sally Price)
Date: Fri May 14 06:50:28 2010
Subject: [Histonet] Best charged slides for IHC
Message-ID: 

Has anybody done a comparison of charged slides, to see which ones are best
for IHC staining - espcially when slides will be pretreated in a pressure
cooker?
-- 
Sally Price
From ccrowder <@t> vetmed.lsu.edu  Fri May 14 07:16:02 2010
From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder)
Date: Fri May 14 07:17:29 2010
Subject: [Histonet] Tilapia eyes
Message-ID: 

Fish eyes tend to like different temperatures and water content during 
cutting.   Chill the eyes well after facing off, then when cutting dip a 
brush in the warm  water bath and "paint" the lens area, warming it up a 
little and moistening it at the same time.  If the eyes are processed 
properly, you have a better chance of getting a good section.
Cheryl
 
Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences  
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA 70803

225-578-9734
FAX: 225-578-9720
From alicia.moore <@t> thermofisher.com  Fri May 14 08:16:44 2010
From: alicia.moore <@t> thermofisher.com (Moore, Alicia M.)
Date: Fri May 14 08:16:56 2010
Subject: [Histonet] HT/HTL - Field Laboratory Application Specialist
	(Western US)
Message-ID: <3A1F2C433D645140AACC5AD73CFFEE3FEFA4D2A0@USPHO-MXVS01.amer.thermo.com>

My name is Alicia Moore and I support the centralized staffing function within the Specialty Diagnostics Group of Thermo Fisher Scientific.  Thermo Fisher Scientific Inc. (NYSE: TMO - News) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer.

We're currently searching for an HT/HTL - Field Laboratory Application Specialist located in the Western US.  A short description is below as well as my contact information. Please contact me if you're interested in learning more about the opportunity and please pass my contact information to anyone who you believe may be interested and qualified for this challenging yet rewarding opportunity.

The Field Laboratory Applications Specialist position is responsible for providing installation, customer training and product support for Thermo Fisher Scientific Anatomical Pathology instrumentation and consumables in customer facing applications.  Applications support responsibilities extend to internal and external customers, to include, Channel Partners, Customer Service, Instrumentation/Field Service, Marketing and Sales Team. This position requires up to 75% travel.  To view the complete job posting, please visit www.thermofisher.com/careers and enter keyword: 32921.
Best Wishes,

Alicia Moore
Talent Coordinator
Thermo Fisher Scientific
4481 Campus Drive
Kalamazoo, MI 49008
Ph: 269-544-5739
Fax: 269-372-2624
Email: alicia.moore@thermofisher.com

From cpyse <@t> x-celllab.com  Fri May 14 08:41:39 2010
From: cpyse <@t> x-celllab.com (Cynthia Pyse)
Date: Fri May 14 08:41:58 2010
Subject: [Histonet] Is anyone else having an issue with their paraplast?
In-Reply-To: <8A307C4C42FC17478A648E933A64AAA988A67DCFB5@XCHMBCL1.neutzone.bloomhealth.org>
References: <8A307C4C42FC17478A648E933A64AAA988A67DCFB5@XCHMBCL1.neutzone.bloomhealth.org>
Message-ID: <000001caf36b$2e43b580$8acb2080$@com>

I'm relieved that someone else is having the same problem. I was beginning
to think it was my processor. I have already decided to switch paraffin.

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse@x-celllab.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayes,
Sherry
Sent: Thursday, May 13, 2010 11:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Is anyone else having an issue with their paraplast?

We are having an issue with our paraplast (manufacturer McCormick). There
are black flecks and what looks like a dark sand in the paraplast. The issue
has continued for @8 weeks and has happened with several lot numbers. Is
anyone else experiencing this issue?

Sherry S. Hayes
Laboratory Systems Support Coordinator

Bloomington Hospital
601 West Second Street
PO Box 1149
Bloomington, IN 47402
t812.353.5606
f812.353.5584
p812.334.6337

shayes@bloomingtonhospital.org 
bloomingtonhospital.org




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If you are not the intended recipient, you may NOT use, disclose, copy or
disseminate this information.  Please contact the sender by reply e-mail
immediately and destroy all copies of the original message including all
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Bloomington Hospital
P.O. Box 1149
Bloomington, IN 47402
_______________________________________________
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From Loralee_Mcmahon <@t> URMC.Rochester.edu  Fri May 14 09:27:55 2010
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Fri May 14 09:28:01 2010
Subject: [Histonet] Hep C Antibody
Message-ID: 

Hi Histonet,

Wondering if anyone has ever used a antibody for Hep C on formalin fixed paraffin embedded tissue?
If so where did you get it.
Thanks in advance.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From relia1 <@t> earthlink.net  Fri May 14 10:18:10 2010
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Fri May 14 10:18:13 2010
Subject: [Histonet] RELIA Special Job Alert for Immunohistochemistry
	Specialists 5/14/10
Message-ID: 

Hi Histonetters!!
I hope everyone is getting ready for a great weekend!  I have a few
position that I want to tell you about.  All of these position are full
time and permanent and all of these clients offer competitive salaries,
great benefits and relocation assistance.  
I am working with several clients in need of Immunohistochemistry
Expertise.  ASCP HT certification is required and QIHC is a plus.
Basically if you are an ASCP certified tech with a strong background in
Immunohistochemistry who has worked with a variety of antibodies and an
average to high volume of slides per day my clients would love to talk
to you.
Here is a list of the positions:
Immunohistochemistry Specialists
Atlanta, GA - Fast paced State of the Art Pathology Lab
Cape Cod, MA - Hospital Setting
Phoenix, AZ - Growing State of the Art Pathology Lab
Baton Rouge, LA - Fast Paced Private Pathology Lab
 
I also need a strong Grossing Histotechnologist for a client in Atlanta
- this is a night shift position.
I have histotechnician/histotechnologist positions with some great
clients available in AZ, GA, MA, NV and CA  and Manager/Supervisors
positions in CA, AZ, NY and NV.
 
If you or anyone you know might be interested in hearing more about any
of these opportunities please contact me.  I can be reached at
relia1@earthlink.net or toll free at 866-607-3542.  
 
Thanks-Pam
Thank You!
 
Pam Barker
President
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
Toll Free: (866)607-3542
FAX: (407)678-2788
E-mail: relia1@earthlink.net 
<>
www.myspace.com/pamatrelia 
www.twitter.com/pamatrelia

From GDawson <@t> dynacaremilwaukee.com  Fri May 14 10:54:22 2010
From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen)
Date: Fri May 14 10:54:55 2010
Subject: [Histonet] RE: AMT Cover
In-Reply-To: <7B380BF8E6354F4994F20E095D43F171EF6C7458@USPHO-MXVS01.amer.thermo.com>
Message-ID: 

All,

I just looked at the notorious AMT cover that people are up in arms about and I must disagree with the comments that were posted thus far.  The techs here and myself are quite surprised by the reactions because it is on a "medical" catalogue and is far from being lewd or obscene.  You could probably find worse by turning on the TV for 15 minutes or looking at a billboard or two.  

Just Some Wisconsin Input,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

From JCBRITTON <@t> Cheshire-Med.COM  Fri May 14 11:33:13 2010
From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton)
Date: Fri May 14 11:33:19 2010
Subject: [Histonet] RE: AMT Cover
In-Reply-To: 
References: <7B380BF8E6354F4994F20E095D43F171EF6C7458@USPHO-MXVS01.amer.thermo.com>
	
Message-ID: 

 

I second that!

 

Josie Britton HT

Keene, NH

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson,
Glen
Sent: Friday, May 14, 2010 11:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: AMT Cover

 

All,

 

I just looked at the notorious AMT cover that people are up in arms
about and I must disagree with the comments that were posted thus far.
The techs here and myself are quite surprised by the reactions because
it is on a "medical" catalogue and is far from being lewd or obscene.
You could probably find worse by turning on the TV for 15 minutes or
looking at a billboard or two.  

 

Just Some Wisconsin Input,

 

Glen Dawson  BS, HT & QIHC (ASCP)

IHC Manager

Milwaukee, WI

 

_______________________________________________

Histonet mailing list

Histonet@lists.utsouthwestern.edu

http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message.
From Reuel.Cornelia <@t> tsrh.org  Fri May 14 12:42:20 2010
From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia)
Date: Fri May 14 12:42:41 2010
Subject: [Histonet] large fibrous bone tissue
Message-ID: <4BED452C020000C50007890E@mail.TSRH.ORG>

How do you process a fibrous bone tissue ( 7 mm thick).  We have use Paraffin Type 9 from Richard allan Scientific to embed works well with our bone femur( 7 mm) when cutting but on fibrous bone it does not give us a good result in cutting the blocks. It is like cutting a uterus tissue but a little bit harder. Please give me your opinion on how to remedy this kind of tissue not mentioning double embedding method or plastic. Thank you.

Reuel Cornelia



From tanisha.neely <@t> covance.com  Fri May 14 12:47:34 2010
From: tanisha.neely <@t> covance.com (Neely, Tanisha)
Date: Fri May 14 12:47:58 2010
Subject: [Histonet] Grossing Technician Qualifications
Message-ID: <816E3C72F855F14985FC31D7C963AE6F1DA54F4C@indexch03.ent.covance.com>

Hi Histonetters:
 
Are the guidelines for a tech who is strictly limited to grossing
anatomic pathology specimens different than for those of a full time
histotech? Could a bachelor degree'd person with the right course work
qualify for this position? If so, does anyone have documentation of
this? I'd like to present this as a staffing option to my management if
possible. 
 
Thanks for your help.
 
Tanisha Neely HT(ASCP)
 


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for proper delivery, and then please delete the message 
from your inbox. Thank you.

From cmiller <@t> physlab.com  Fri May 14 13:12:45 2010
From: cmiller <@t> physlab.com (Cheri Miller)
Date: Fri May 14 13:12:51 2010
Subject: [Histonet] (no subject)
Message-ID: 

Is anyone using Surgipath's Select Tech H&E kit? I have a sample kit and I want to try it out on my Leica XL autostainer.  I am looking for a protocol with times/dips etc. Thanks,

Cheryl A. Miller HT ASCPcm
Histology Supervisor
Physicians Laboratory, P.C.
402 493 0403

________________________________
PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system.
From hardwired <@t> techie.com  Fri May 14 13:32:38 2010
From: hardwired <@t> techie.com (hardwired@techie.com)
Date: Fri May 14 13:33:06 2010
Subject: [Histonet] traveling histotechs
Message-ID: <8CCC1AAF7314BBB-1734-11B4C@web-mmc-d05.sysops.aol.com>

I was wondering if anyone has any information on traveling histology technician companys.  I wish to try the traveling tech world and do not know how to get started.  Thanks


From histobr <@t> yahoo.com  Fri May 14 13:59:41 2010
From: histobr <@t> yahoo.com (Ruser Rebecca)
Date: Fri May 14 13:59:44 2010
Subject: [Histonet] available positions in Dallas
Message-ID: <635953.58754.qm@web56501.mail.re3.yahoo.com>

I have 2 full time positions available.? We have a newly created position for a histotech with experience for an 11:00 p.m to 7:30 a.m. shift.? We also have a position available at our private laboratory for a histotech with immuno experience.? If you would be interested in either of these positions, please go to Methodist? Health Systems and apply online.? You can also contact me at rebeccaruser@mhd.com.
Rebecca Ruser
Methodist Dallas Medical Center
214-947-3538


      
From tahseen <@t> brain.net.pk  Fri May 14 14:27:59 2010
From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk)
Date: Fri May 14 14:28:06 2010
Subject: [Histonet] IHC marker for carbonic anhydrase 9
Message-ID: <1071.202.125.145.178.1273865279.squirrel@brain.net.pk>

Dear all
We are locking immuno marker for carbonic anhydrase 9 on p.section.
Thanks advance
Muhammad Tahseen
Histology Supervisor
SKMCH&RC
Lahore Pakistan



From pruegg <@t> ihctech.net  Fri May 14 14:37:13 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Fri May 14 14:38:21 2010
Subject: [Histonet] Are Histotechs considered "exempt" employees?
In-Reply-To: <2842DC75AE43AA4B92954CFB31781BC105697FAC@CHW-MSG-301.chw.edu>
References: ,
	<2842DC75AE43AA4B92954CFB31781BC105697FA5@CHW-MSG-301.chw.edu>
	<2842DC75AE43AA4B92954CFB31781BC105697FAC@CHW-MSG-301.chw.edu>
Message-ID: 

Wages are totally region specific, in Colorado the cost of living is not as
high as it is in CA for sure and many other places, plus since Colorado is a
desirable place to live we usually don't have trouble getting people to come
here to work, so it is a supply and demand issue.  It keeps our wages lower
than some other places but it is a trade off (we get to live in CO), there
are some places I wouldn't not go to work no matter how much they paid me
and I bet they do have to pay more because they don't have people clamoring
to live there.  And then there are places like SF that have to pay more
because you need more to live there or in the area (most people in SF live
far away from where they work and have huge commutes,which is another
undesirable issue in my mind).  It doesn't make any sense to compare wages
from region to region without taking these things into consideration.

Regards,
Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford,
Karen - SMMC-SF
Sent: Thursday, May 13, 2010 6:10 AM
To: joelle weaver
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?

It's just crazy the wage differences around the country.  But we should
fight for the higher wages in whatever location we live in.  Not
everyone can do this type of job and be good at it.  

 

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-668-1000 ext. 6167

________________________________

From: joelle weaver [mailto:joelleweaver@hotmail.com] 
Sent: Thursday, May 13, 2010 4:24 AM
To: Heckford, Karen - SMMC-SF; histonet-bounces@lists.utsouthwestern.edu
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?

 

Boy, I wish! I live in Ohio with over 10 years bench experience,
supervisory experience, HTL and almost a masters (4 classes left)- and I
don't approach the HT scale you proposed-even the minimum.
 
> Date: Tue, 11 May 2010 05:15:40 -0700
> From: Karen.Heckford@CHW.edu
> To: sandoval.1965@hotmail.com
> Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
> CC: histonet@lists.utsouthwestern.edu
> 
> $16.15 per hour. They are getting a great deal. I believe you should
> be making at least double that. What I believe is happening is that a
> lot of offices are now starting to open their own labs and they are
> trying to get us cheap. We all need to stick to our guns and not let
> them get us cheaper than we are worth. Yes, I realize that San
> Francisco is more but the starting wage for a HT's should be $25.00 to
> $30.00 an hour to start for a newly certified tech. No matter of
> location in California. You can always negotiate higher if you have
> more experience and more certifications.
> 
> Just my two cents worth,
> 
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Anthony
> Sandoval
> Sent: Monday, May 10, 2010 9:01 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Are Histotechs considered "exempt" employees?
> 
> Hello fellow Histotechs, I have recently become certified as an HTL
and
> was 
> wondering if anyone out there is an 'exempt' employee? I live in
> California 
> and feel that I am being taken advantage of. I make 16.15$ per hour
and
> 
> frequently work 50 hour weeks. Am I off base? should I just be
grateful
> that 
> I have a job, as my employer so frequently reminds me? Thank you
> Histonet! 
> you have been an invaluable resource in my career and assisting me in 
> passing the HTL! 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________

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inbox. Learn more.
 

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From Samuel_Perry <@t> DFCI.HARVARD.EDU  Fri May 14 15:16:06 2010
From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel)
Date: Fri May 14 15:16:12 2010
Subject: [Histonet] Prolonged FFPE slide and block storage
Message-ID: 

Hi All,
We have a growing need for storing slides. 

They need to be stored in a way that they won't become oxidized and loose their
antigenicity, since we use them for IHC.  

Any suggestions for inexpensive methods for prolonged storage of FFPE slides and
blocks is greatly appreciated.

Thanks!

Sam Perry

Research Technician 
Dana-Farber Cancer Institute
Boston, MA


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.
From Herrick.James <@t> mayo.edu  Fri May 14 15:22:50 2010
From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim))
Date: Fri May 14 15:23:39 2010
Subject: [Histonet] Mounting Medium Issues
Message-ID: <7267A64D75F58241B577876D8A885631015A9AE2@msgebe41>

Hello everyone,

Hope you have all had a good week!!

Has anyone had any negative experiences using Eukitt as your mounting
media? We embed our specimens (human - iliac crest, animal - femurs,
tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period
of time that a fairly large number of our slides are beginning to show
signs of the medium breaking up below the cover slip. It looks as if
there are large air pockets left behind. We are not yet sure why this is
happening. Would any of you geniuses have a resolution for us or know of
a better mounting media to use with plastic embedded specimens -
brightfield and fluorescence? Thanks for your expertise. It is very much
appreciated.

Have a great weekend!!

Jim


From Timothy.Morken <@t> ucsfmedctr.org  Fri May 14 16:05:48 2010
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim)
Date: Fri May 14 16:06:01 2010
Subject: [Histonet] RE: Prolonged FFPE slide and block storage
In-Reply-To: 
References: 
Message-ID: <1AAF670737F193429070841C6B2ADD4C018798D642@EXMBMCB15.ucsfmedicalcenter.org>

I have a paper from Yale several years ago about their tissue array facility. They found that storage in nitrogen helped a lot. I will try to find it, but you may be able to google it faster. 

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Samuel
Sent: Friday, May 14, 2010 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Prolonged FFPE slide and block storage

Hi All,
We have a growing need for storing slides. 

They need to be stored in a way that they won't become oxidized and loose their
antigenicity, since we use them for IHC.  

Any suggestions for inexpensive methods for prolonged storage of FFPE slides and
blocks is greatly appreciated.

Thanks!

Sam Perry

Research Technician 
Dana-Farber Cancer Institute
Boston, MA


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From sgoebel <@t> xbiotech.com  Fri May 14 16:37:19 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Fri May 14 16:37:24 2010
Subject: [Histonet] IHC
Message-ID: <20100514143719.9e2d9aa830e8449a2412eb1e4f2f067e.d8ea2310fa.wbe@email04.secureserver.net>


   Can  you  leave  your  primary antibody on slides overnight (or the w   eekend)  or  will  this  screw  things  up?   I  don't want to be here
   another
   
   Sarah Goebel, B.A., HT (ASCP)

   <
   XBiotech USA Inc.
   8201 East Riverside Dr. Bldg 4 Suit
   Austin, Texas  78744

   (512)386-5107
From JGarfield <@t> lifecell.com  Fri May 14 17:08:04 2010
From: JGarfield <@t> lifecell.com (Garfield, Jacqueline)
Date: Fri May 14 17:08:08 2010
Subject: [Histonet] IHC
Message-ID: <5476245379016B4D8212E8BCD11ECFFBBC5DEECD@AMWPVEX01.kci.com>

Only if the procedure calls for it.  If not, then you can try holding the slides in buffer and refrigerate.  Just be sure to allow the slides to come to room temperature before resuming.  


----- Original Message -----
From: histonet-bounces@lists.utsouthwestern.edu 
To: histonet@lists.utsouthwestern.edu 
Sent: Fri May 14 16:37:19 2010
Subject: [Histonet] IHC


   Can  you  leave  your  primary antibody on slides overnight (or the w   eekend)  or  will  this  screw  things  up?   I  don't want to be here
   another=uple of hours for secondary, etc. on a Friday!!

   
   Sarah Goebel, B.A., HT (ASCP)

   <=H=totechnician

   XBiotech USA Inc.
   8201 East Riverside Dr. Bldg 4 Suit?00

   Austin, Texas  78744

   (512)386-5107
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From saby_joseph_a <@t> yahoo.com  Fri May 14 17:21:04 2010
From: saby_joseph_a <@t> yahoo.com (Joseph Saby)
Date: Fri May 14 17:21:16 2010
Subject: [Histonet] Prolonged FFPE slide and block storage
In-Reply-To: 
References: 
Message-ID: <538179.1555.qm@web114409.mail.gq1.yahoo.com>

Sam-

Are you storing sectioned slides?

What we do is to deparaffinize and coverslip sectioned but unstained slides.? I haven' had to go back, but I believe they will store that way a long time.

Joe Saby, BA HT




________________________________
From: "Perry, Samuel" 
To: histonet@lists.utsouthwestern.edu
Sent: Fri, May 14, 2010 4:16:06 PM
Subject: [Histonet] Prolonged FFPE slide and block storage

Hi All,
We have a growing need for storing slides. 

They need to be stored in a way that they won't become oxidized and loose their
antigenicity, since we use them for IHC.? 

Any suggestions for inexpensive methods for prolonged storage of FFPE slides and
blocks is greatly appreciated.

Thanks!

Sam Perry

Research Technician 
Dana-Farber Cancer Institute
Boston, MA


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From anonwums1 <@t> gmail.com  Fri May 14 17:22:21 2010
From: anonwums1 <@t> gmail.com (Adam .)
Date: Fri May 14 17:22:27 2010
Subject: [Histonet] IHC
In-Reply-To: <20100514143719.9e2d9aa830e8449a2412eb1e4f2f067e.d8ea2310fa.wbe@email04.secureserver.net>
References: <20100514143719.9e2d9aa830e8449a2412eb1e4f2f067e.d8ea2310fa.wbe@email04.secureserver.net>
Message-ID: 

I always leave my primary antibodies on overnight at 4C. I've seen some
protocols for tricky antigens where people leave it on for days. I wouldn't
leave it a room temperature though.

Go home and enjoy your Friday,
Adam

On Fri, May 14, 2010 at 4:37 PM,  wrote:

>
>   Can  you  leave  your  primary antibody on slides overnight (or the w
> eekend)  or  will  this  screw  things  up?   I  don't want to be here
>   another couple of hours for secondary, etc. on a Friday!!
>
>
>   Sarah Goebel, B.A., HT (ASCP)
>
>   < i>H istotechnician
>
>   XBiotech USA Inc.
>
>   8201 East Riverside Dr. Bldg 4 Suit e 100
>
>   Austin, Texas  78744
>
>   (512)386-5107
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From andreahooper <@t> rocketmail.com  Fri May 14 18:18:01 2010
From: andreahooper <@t> rocketmail.com (andreahooper@rocketmail.com)
Date: Fri May 14 18:18:16 2010
Subject: [Histonet] IHC
In-Reply-To: <20100514143719.9e2d9aa830e8449a2412eb1e4f2f067e.d8ea2310fa.wbe@email04.secureserver.net>
References: <20100514143719.9e2d9aa830e8449a2412eb1e4f2f067e.d8ea2310fa.wbe@email04.secureserver.net>
Message-ID: <1670345416-1273879082-cardhu_decombobulator_blackberry.rim.net-514854825-@bda2667.bisx.prod.on.blackberry>

Overnight is surely fine but make sure at 4 deg C for two reasons. First you want to minimize any bacterial growth. Second you want to ensure it doesn't evaporate significantly which would change your antibody concentration. For that same reason make sure you humidify the chamber amply as well as don't skimp on the volume applied to the slide - as even humidified and at 4 deg C eventually everything evaporates (say if you left it for days).

If you routinely do this ab for 1h RT and are now switching to overnight be aware you may (or may not) get more staining or nonspecific binding. The titration of ab could be a little different when optimiZed for overnight.

That being said, I say go for it.

Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: sgoebel@xbiotech.com
Date: Fri, 14 May 2010 14:37:19 
To: 
Subject: [Histonet] IHC


   Can  you  leave  your  primary antibody on slides overnight (or the w   eekend)  or  will  this  screw  things  up?   I  don't want to be here
   anothercouple of hours for secondary, etc. on a Friday!!

   
   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician

   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 Suite 100

   Austin, Texas  78744

   (512)386-5107
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From louise.renton <@t> gmail.com  Sat May 15 04:30:17 2010
From: louise.renton <@t> gmail.com (louise renton)
Date: Sat May 15 04:30:21 2010
Subject: [Histonet] large fibrous bone tissue
In-Reply-To: <4BED452C020000C50007890E@mail.TSRH.ORG>
References: <4BED452C020000C50007890E@mail.TSRH.ORG>
Message-ID: 

I have found this helps.....
1. Embed the tissue in  a dep mould, as this provides more stability, then
2. Face the block
3.. leave in -20 deg freezer overnight
4. remove from freezer and cut sections
5. If you have multiple blocks to work with, leave them in the freezer until
ready to cut

regards
On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia wrote:

> How do you process a fibrous bone tissue ( 7 mm thick).  We have use
> Paraffin Type 9 from Richard allan Scientific to embed works well with our
> bone femur( 7 mm) when cutting but on fibrous bone it does not give us a
> good result in cutting the blocks. It is like cutting a uterus tissue but a
> little bit harder. Please give me your opinion on how to remedy this kind of
> tissue not mentioning double embedding method or plastic. Thank you.
>
> Reuel Cornelia
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
From rjbuesa <@t> yahoo.com  Sat May 15 08:36:03 2010
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Sat May 15 08:36:08 2010
Subject: [Histonet] RE: Prolonged FFPE slide and block storage
In-Reply-To: <1AAF670737F193429070841C6B2ADD4C018798D642@EXMBMCB15.ucsfmedicalcenter.org>
Message-ID: <601840.54617.qm@web65704.mail.ac4.yahoo.com>

Inexpensive long term anti oxidizing storage? Immerse them in mineral oil!
Ren? J.

--- On Fri, 5/14/10, Morken, Tim  wrote:


From: Morken, Tim 
Subject: [Histonet] RE: Prolonged FFPE slide and block storage
To: "Perry, Samuel" , "histonet@lists.utsouthwestern.edu" 
Date: Friday, May 14, 2010, 5:05 PM


I have a paper from Yale several years ago about their tissue array facility. They found that storage in nitrogen helped a lot. I will try to find it, but you may be able to google it faster. 

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA? 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Samuel
Sent: Friday, May 14, 2010 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Prolonged FFPE slide and block storage

Hi All,
We have a growing need for storing slides. 

They need to be stored in a way that they won't become oxidized and loose their
antigenicity, since we use them for IHC.? 

Any suggestions for inexpensive methods for prolonged storage of FFPE slides and
blocks is greatly appreciated.

Thanks!

Sam Perry

Research Technician 
Dana-Farber Cancer Institute
Boston, MA


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From hfedor <@t> jhmi.edu  Sat May 15 09:04:07 2010
From: hfedor <@t> jhmi.edu (Helen Fedor)
Date: Sat May 15 09:04:12 2010
Subject: [Histonet] RE: Prolonged FFPE slide and block storage
In-Reply-To: <601840.54617.qm@web65704.mail.ac4.yahoo.com>
References: <1AAF670737F193429070841C6B2ADD4C018798D642@EXMBMCB15.ucsfmedicalcenter.org>
	<601840.54617.qm@web65704.mail.ac4.yahoo.com>
Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D316C011C79B@JHEMTEXVS3.win.ad.jhu.edu>

Hello, We have done a study and found that storage in small zip-loc bags at -20 is an inexpensive and convenient way to store slides. In fact the slides stored for 5 years at -20 stain better than freshly cut sections from the same blocks. This was also published recently by others. Dr Rimm at Yale does have the storage under nitrogen gas system that is available commercially.


Helen L. Fedor 

Tissue Microarray Lab, Manager
Prostate Spore Lab, Manager
Johns Hopkins University
600 N. Wolfe St,?| Marburg Room 406
Baltimore, MD?| 21287-7065

410.614.1660




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Saturday, May 15, 2010 9:36 AM
To: SamuelPerry; histonet@lists.utsouthwestern.edu; TimMorken
Subject: Re: [Histonet] RE: Prolonged FFPE slide and block storage

Inexpensive long term anti oxidizing storage? Immerse them in mineral oil!
Ren? J.

--- On Fri, 5/14/10, Morken, Tim  wrote:


From: Morken, Tim 
Subject: [Histonet] RE: Prolonged FFPE slide and block storage
To: "Perry, Samuel" , "histonet@lists.utsouthwestern.edu" 
Date: Friday, May 14, 2010, 5:05 PM


I have a paper from Yale several years ago about their tissue array facility. They found that storage in nitrogen helped a lot. I will try to find it, but you may be able to google it faster. 

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA? 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Samuel
Sent: Friday, May 14, 2010 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Prolonged FFPE slide and block storage

Hi All,
We have a growing need for storing slides. 

They need to be stored in a way that they won't become oxidized and loose their
antigenicity, since we use them for IHC.? 

Any suggestions for inexpensive methods for prolonged storage of FFPE slides and
blocks is greatly appreciated.

Thanks!

Sam Perry

Research Technician 
Dana-Farber Cancer Institute
Boston, MA


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From pruegg <@t> ihctech.net  Sat May 15 09:07:45 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sat May 15 09:08:34 2010
Subject: SPAM-LOW:  Re: [Histonet] IHC
In-Reply-To: <1670345416-1273879082-cardhu_decombobulator_blackberry.rim.net-514854825-@bda2667.bisx.prod.on.blackberry>
References: <20100514143719.9e2d9aa830e8449a2412eb1e4f2f067e.d8ea2310fa.wbe@email04.secureserver.net>
	<1670345416-1273879082-cardhu_decombobulator_blackberry.rim.net-514854825-@bda2667.bisx.prod.on.blackberry>
Message-ID: 

I do this all the time, but I do put a coverslip over the section and keep
it in a humid chamber.  We leave it on the counter with a diaper over it and
an ice pack sitting on top.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
andreahooper@rocketmail.com
Sent: Friday, May 14, 2010 5:18 PM
To: sgoebel@xbiotech.com; histonet-bounces@lists.utsouthwestern.edu;
histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] IHC

Overnight is surely fine but make sure at 4 deg C for two reasons. First you
want to minimize any bacterial growth. Second you want to ensure it doesn't
evaporate significantly which would change your antibody concentration. For
that same reason make sure you humidify the chamber amply as well as don't
skimp on the volume applied to the slide - as even humidified and at 4 deg C
eventually everything evaporates (say if you left it for days).

If you routinely do this ab for 1h RT and are now switching to overnight be
aware you may (or may not) get more staining or nonspecific binding. The
titration of ab could be a little different when optimiZed for overnight.

That being said, I say go for it.

Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: sgoebel@xbiotech.com
Date: Fri, 14 May 2010 14:37:19 
To: 
Subject: [Histonet] IHC


   Can  you  leave  your  primary antibody on slides overnight (or the w
eekend)  or  will  this  screw  things  up?   I  don't want to be here
   anothercouple of hours for secondary, etc. on a Friday!!

   
   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician

   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 Suite 100

   Austin, Texas  78744

   (512)386-5107
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From pruegg <@t> ihctech.net  Sat May 15 09:12:02 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sat May 15 09:12:52 2010
Subject: SPAM-LOW:  Re: [Histonet] Prolonged FFPE slide and block storage
In-Reply-To: <538179.1555.qm@web114409.mail.gq1.yahoo.com>
References: 
	<538179.1555.qm@web114409.mail.gq1.yahoo.com>
Message-ID: 

To store cut sections for use later for IHC I do not bake them to melt the
paraffin, they keep better, melt them just before use.  I also store the cut
slides at 4dc.  For really precious control blocks, I reseal them with
paraffin (just dip in hot paraffin and then cool) and I do also store the
blocks as 4dc.  We just ran into a problem with tonsil sections going bad
for IMP3 ab., this is a real concern.

Best regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph Saby
Sent: Friday, May 14, 2010 4:21 PM
To: Perry, Samuel; histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] Prolonged FFPE slide and block storage

Sam-

Are you storing sectioned slides?

What we do is to deparaffinize and coverslip sectioned but unstained
slides.? I haven' had to go back, but I believe they will store that way a
long time.

Joe Saby, BA HT




________________________________
From: "Perry, Samuel" 
To: histonet@lists.utsouthwestern.edu
Sent: Fri, May 14, 2010 4:16:06 PM
Subject: [Histonet] Prolonged FFPE slide and block storage

Hi All,
We have a growing need for storing slides. 

They need to be stored in a way that they won't become oxidized and loose
their
antigenicity, since we use them for IHC.? 

Any suggestions for inexpensive methods for prolonged storage of FFPE slides
and
blocks is greatly appreciated.

Thanks!

Sam Perry

Research Technician 
Dana-Farber Cancer Institute
Boston, MA


The information in this e-mail is intended only for the person to whom it is
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contains patient information, please contact the Partners Compliance
HelpLine at
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From pruegg <@t> ihctech.net  Sat May 15 09:17:50 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sat May 15 09:18:36 2010
Subject: SPAM-LOW: Re: [Histonet] RE: Prolonged FFPE slide and block
	storage
In-Reply-To: <601840.54617.qm@web65704.mail.ac4.yahoo.com>
References: <1AAF670737F193429070841C6B2ADD4C018798D642@EXMBMCB15.ucsfmedicalcenter.org>
	<601840.54617.qm@web65704.mail.ac4.yahoo.com>
Message-ID: 

Yuk Rene, that sounds so messy, but it is cheap except for tying up racks
and buckets.

Cheers,
Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Saturday, May 15, 2010 7:36 AM
To: SamuelPerry; histonet@lists.utsouthwestern.edu; TimMorken
Subject: SPAM-LOW: Re: [Histonet] RE: Prolonged FFPE slide and block storage

Inexpensive long term anti oxidizing storage? Immerse them in mineral oil!
Ren? J.

--- On Fri, 5/14/10, Morken, Tim  wrote:


From: Morken, Tim 
Subject: [Histonet] RE: Prolonged FFPE slide and block storage
To: "Perry, Samuel" ,
"histonet@lists.utsouthwestern.edu" 
Date: Friday, May 14, 2010, 5:05 PM


I have a paper from Yale several years ago about their tissue array
facility. They found that storage in nitrogen helped a lot. I will try to
find it, but you may be able to google it faster. 

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA? 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry,
Samuel
Sent: Friday, May 14, 2010 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Prolonged FFPE slide and block storage

Hi All,
We have a growing need for storing slides. 

They need to be stored in a way that they won't become oxidized and loose
their
antigenicity, since we use them for IHC.? 

Any suggestions for inexpensive methods for prolonged storage of FFPE slides
and
blocks is greatly appreciated.

Thanks!

Sam Perry

Research Technician 
Dana-Farber Cancer Institute
Boston, MA


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail
contains patient information, please contact the Partners Compliance
HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in
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but does not contain patient information, please contact the sender and
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From Laura.Miller <@t> leica-microsystems.com  Sat May 15 16:01:05 2010
From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com)
Date: Sat May 15 16:01:11 2010
Subject: [Histonet] Laura Miller is Out of  the Office.
Message-ID: 


I will be out of the office starting  05/14/2010 and will not return until
05/18/2010.

I am out sick today. I will reply to your message on Monday.


______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
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From rsrichmond <@t> gmail.com  Sat May 15 16:46:58 2010
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Sat May 15 16:47:02 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
Message-ID: 

Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is
strictly limited to grossing
anatomic pathology specimens different than for those of a full time
histotech? Could a bachelor degree'd person with the right course work
qualify for this position? If so, does anyone have documentation of
this? I'd like to present this as a staffing option to my management
if possible.<<

Well, I'm giving a talk on the subject to the Tennessee Society for
Histotechnology meeting in Chattanooga, and I'm pretty confused about
the question of who's allowed to gross - the rules seem to be
changing, and different certifying organizations have different
requirements. If somebody can spell out in detail who it is that's
requiring what, I'd appreciate it.

Bob Richmond
Samurai Pathologist
Knoxville TN

From Eric.Hoy <@t> UTSouthwestern.edu  Sat May 15 18:59:26 2010
From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy)
Date: Sat May 15 18:59:44 2010
Subject: [Histonet] Re: IHC incubations
In-Reply-To: <201005151722.o4FHMpoD025632@flpd244.prodigy.net>
Message-ID: 

Sarah Goebel asked about prolonged incubation of primary antibodies on an
IHC.

You can do this, usually without increased background or non-specific
staining, but it is imperative that you validate the procedure in comparison
to the published method that you are using.  This will require you to spend
the extra hours in the lab, at least once, so that you perform the test as
the procedure indicates.  I would set up the same slides/primary when I was
doing this, and let them incubate overnight.

You also need to carefully define (and validate) what "overnight" means.  Is
it 12 hours? 18 hours?  24 hours?  I like to establish a range, so I can
state in my procedure, "The slides are incubated for 20 hours, plus or minus
two hours, at room temperature in a humidified chamber."

I have been doing IHC for over 30 years, and this type of validation has
always kept the inspectors happy.  More importantly, it gives me the
latitude to do a two hour procedure or an overnight procedure for the same
antibody/tissue combination.

Happy staining!

Eric Hoy

===============================================
Eric S. Hoy, Ph.D., SI(ASCP)
Clinical Associate Professor
Department of Medical Laboratory Sciences
The University of Texas Southwestern Medical Center
Dallas, Texas
Email: Eric.Hoy@UTSouthwestern.edu
===============================================





From Lesley.Bechtold <@t> jax.org  Mon May 17 07:33:37 2010
From: Lesley.Bechtold <@t> jax.org (Lesley Bechtold)
Date: Mon May 17 07:33:47 2010
Subject: [Histonet] Histotechology Position in Bar Harbor, Maine
Message-ID: <3BDA51FFD1A83B4E90829F594A5C371742A0E8D7D2@JAXBHMAIL01.jax.org>

Histotechnologist I/II

A fulltime Histotechnologist I/II position is available in the Histology Service of The Jackson Laboratory.  Successful applicants will be required to conduct standard histological protocols such as embedding, sectioning and staining, as well as routine laboratory maintenance and administrative tasks.  Qualified applicants will possess, at a  minimum, an Associate's degree in a biological science and HT (ASCP) certification plus 2 years of experience in histology OR a Bachelor's degree in a biological field and a minimum 2 years of experience in histology.  Applicants must demonstrate facility with email, internet, word processing, spreadsheets and familiarity with database operations.  Effective written and verbal communication skills are essential.  Successful applicants will demonstrate good interpersonal skills and must have the ability and willingness to function effectively in a team environment. Experience in murine histology and specialized techniques such as serial sectioning, immunohistochemistry, plastic embedding and plastic sectioning are a plus.  Given the dynamic nature of basic research incumbents must be open to furthering their practical skills and theory training.

The Jackson Laboratory is one of the world's foremost centers for mammalian genetics research. Located in Bar Harbor, Maine, the lab is adjacent to Acadia National Park. Mountains, ocean, forests, lakes, and trails are all within walking distance. If you are looking for a more natural environment, this could be the opportunity you've been searching for.

Interested individuals should apply on-line at www.jax.org/careers.  Refer to job requisition #2384.  Please submit cover letter and resume as one document.




From BenatM <@t> gosh.nhs.uk  Mon May 17 08:55:31 2010
From: BenatM <@t> gosh.nhs.uk (Malika Benatti)
Date: Mon May 17 08:56:15 2010
Subject: [Histonet] Alpha Dystroglycan Immuno
Message-ID: <4BF158EB.4626.0038.0@gosh.nhs.uk>

** Proprietary **
** Reply Requested When Convenient **


Having issue with Millipore Alpha Dystroglycan antibody.

Millipore recommend 1:100 dilution so we  tried various titrations ranging from 1:100, 3:200 to 9:200, normal and abnormal muscle tissue with very poor results. After staining up to  9:200 dilution immuno come out patchy not strong enough

Any one on the histonet list use this antibody, if so at what dilution.

any input would be really appreciated

Malika



Malika Benatti
 
Specialist BMS 
Camelia Botnar Laboratories
Histopathology Department
Great Ormond Street Hospital
London WC1N 3JH

 
Tel:  +44 20 7405 9200 ext 5475
Fax:  +44 20 7829 7875
 
benatm@gosh.nhs.uk


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From cbarone <@t> NEMOURS.ORG  Mon May 17 09:13:53 2010
From: cbarone <@t> NEMOURS.ORG (Barone, Carol )
Date: Mon May 17 09:14:06 2010
Subject: [Histonet] Region II Meeting Atlantic City, NJ
Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7D14@wlmmsx01.nemours.org>

Histonetter's: June 10-12, 2010 Clarion Hotel & Convention Center ... 

Our Featured Speaker this year is Peggy Wenk. She is the Director of the
HT/HTL Program at William Beaumont Hospital in Royal Oak, MI. In 2007
Peggy received the NSH Histotechnologist of the Year Award, is currently
the Teleconference Coordinator, and over the years has held many other
positions supporting NSH. She is an outstanding example of someone
committed to the education of histologists nationwide! Peggy will be
presenting on Friday and Saturday on the following topics: Sectioning
Artifacts, Troubleshooting Amyloid Staining, Reprocessing, Ionizing
Radiation Safety. You don't want to miss this opportunity to learn from
one of the histology icons of our time!

You can learn about other great seminars and workshops by downloading a
meeting brochure from the NSH website under state meeting information at
www.nsh.org/content/region-ii-meeting.

From Bill.Tench <@t> pph.org  Mon May 17 09:30:40 2010
From: Bill.Tench <@t> pph.org (Tench, Bill)
Date: Mon May 17 09:31:28 2010
Subject: [Histonet] Grossing assistants
In-Reply-To: <20100516172350.2C5C4686A6@mail1.pph.org>
References: <20100516172350.2C5C4686A6@mail1.pph.org>
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD028630CD@MAIL1.pph.local>

The "rules" are established by CMS through CLIA regulations.  They are
applied based on the "complexity" of the activity.  Grossing is
considered "high complexity" and thus is subject to rather stringent
rules (which become more complicated because there are "grandfather"
clauses).  For years, the CAP took a more "liberal" view of how various
parts of the grossing activity could be defined.  CMS reviews the CAP
standards.  So, the result is a "tightening" of those standards (ie,
don't blame the CAP for this one). The CAP has provided rather clear cut
guidelines, and if you find them confusing, then you may contact the LAP
program there and someone will assist you. You may ask the CAP to lobby
for you (which I suspect they have done) or you can appeal to CMS (which
will largely not listen to anything reasonable, and if it does, will
tell you it will take multiple years to make any changes).

Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Sunday, May 16, 2010 10:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [BULK] Histonet Digest, Vol 78, Issue 22


----------------------------------------------------------------------



------------------------------

Message: 2
Date: Sat, 15 May 2010 17:46:58 -0400
From: Robert Richmond 
Subject: [Histonet] Re: Grossing Technician Qualifications
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is
strictly limited to grossing
anatomic pathology specimens different than for those of a full time
histotech? Could a bachelor degree'd person with the right course work
qualify for this position? If so, does anyone have documentation of
this? I'd like to present this as a staffing option to my management
if possible.<<

Well, I'm giving a talk on the subject to the Tennessee Society for
Histotechnology meeting in Chattanooga, and I'm pretty confused about
the question of who's allowed to gross - the rules seem to be
changing, and different certifying organizations have different
requirements. If somebody can spell out in detail who it is that's
requiring what, I'd appreciate it.

Bob Richmond
Samurai Pathologist
Knoxville TN



------------------------------

****************************************

mail2.pph.org made the following annotations
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From sgoebel <@t> xbiotech.com  Mon May 17 10:05:32 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Mon May 17 10:06:03 2010
Subject: [Histonet] CD14
Message-ID: <20100517080532.9e2d9aa830e8449a2412eb1e4f2f067e.546f8c32e6.wbe@email04.secureserver.net>


   Has anybody messed with CD14 before with ICC?  It looks like   blue  with  no  DAB  in  sight!!   If  anyone  has  an ICC image (non   -flourescence)  I  would heart you forever...and yes I stayed Friday 2
   hours 
   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician
   
   XBiotech U
   8201 East Riverside Dr. Bldg
   Austin, Texas  787
   (512)386-5107
From kmerriam2003 <@t> yahoo.com  Mon May 17 10:13:40 2010
From: kmerriam2003 <@t> yahoo.com (Kim Merriam)
Date: Mon May 17 10:13:43 2010
Subject: [Histonet] Vector ABC-HRP
Message-ID: <761967.43889.qm@web50301.mail.re2.yahoo.com>

Hi?All,

This may?seem like a no-brainer, but can I dilute Vector's ABC-HRP solution in TBS instead of PBS?? I?have always used PBS, but we are looking to avoid PBS?entirely for one of my projects.

Had anyone used?TBS?to?dilute ABC-HRP?

Kim
?Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 


      
From dmccaig <@t> ckha.on.ca  Mon May 17 10:26:53 2010
From: dmccaig <@t> ckha.on.ca (Diana McCaig)
Date: Mon May 17 10:27:08 2010
Subject: [Histonet] processing schedule for needle core biopsies
In-Reply-To: <3BDA51FFD1A83B4E90829F594A5C371742A0E8D7D2@JAXBHMAIL01.jax.org>
References: <3BDA51FFD1A83B4E90829F594A5C371742A0E8D7D2@JAXBHMAIL01.jax.org>
Message-ID: 

We have been notices folds and wrinkles in the needle cores biopsies
that do not seem to be affected by increased soaking. I am inclined to
think it has to with processing.  Currently they are done with the
routine surgical specimens .What is the preferred schedule for needle
cores?  Is it best to excluded them from the main processor and do a
separate run with reduced times?
thanks
Diana

From cls71877 <@t> sbcglobal.net  Mon May 17 11:08:38 2010
From: cls71877 <@t> sbcglobal.net (Cristi stephenson)
Date: Mon May 17 11:08:42 2010
Subject: [Histonet] Thank you!
Message-ID: <131916.82835.qm@web81202.mail.mud.yahoo.com>

Good morning!
I jsut want to thank everybody for the responses regarding gastric biopsies and "up-front" staining.? All of your help should help me move in the direction i would like to go!
Sincerely,
Cristi
From louise.renton <@t> gmail.com  Mon May 17 12:30:30 2010
From: louise.renton <@t> gmail.com (louise renton)
Date: Mon May 17 12:30:43 2010
Subject: [Histonet] TDE decalcifier
Message-ID: 

hi all,
although I have been using the TDE decalcification system for awhile, I was
hoping to get some answers from the community. are there any thoughts as to
what the solution is?. And how do you tell if  the solution is "used up" or
not. I wonder if anybody out there has some idea as to whether the solution
is "saturated" with calcium salt or not. as always, I look forward to some
answers.
Best regards
-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
From mpowers <@t> dpspa.com  Mon May 17 13:12:36 2010
From: mpowers <@t> dpspa.com (Marian Powers)
Date: Mon May 17 13:12:48 2010
Subject: [Histonet] BCR-ABL
Message-ID: 

Hi:  Is anyone running florescence in situ to detect BCR-ABL?  If so, what
kit are you using?  Thanks in advance.



-- 
Marian L. Powers, HT(ASCP)
Manager, Technical Operations

Doctors Pathology Services
1253 College Park Drive
Dover, DE 19904
302-677-0000-office
302-747-0580-cell

The information contained in this message may be privileged, confidential,
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From Kim.Donadio <@t> bhcpns.org  Mon May 17 14:55:33 2010
From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org)
Date: Mon May 17 14:55:52 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: 
Message-ID: 

There has been a lot of confusion about this. From my understanding the 
real definition of who can gross falls under the "High Complexity" testing 
category. This would be different for different states I think as well. 
Let me give an example. 

In the State of Florida pervious technicians may have been trained to 
Gross large specimens( or biopsies ). With this new law a technician in 
the state of Florida would not be able to continue to gross because in 
actuality they never held a license that allowed them to perform "High 
Complexity" testing. So they are not grandfathered in because of that 
clause. Technologist would, because they have always been able to preform 
"High Complexity" testing. 

I also think that which ever laws your state goes by for people who can 
perform "High Complexity" testing is your answer. 

I hope this helps. 



Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



Robert Richmond  
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/15/2010 04:46 PM

To
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] Re: Grossing Technician Qualifications






Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is
strictly limited to grossing
anatomic pathology specimens different than for those of a full time
histotech? Could a bachelor degree'd person with the right course work
qualify for this position? If so, does anyone have documentation of
this? I'd like to present this as a staffing option to my management
if possible.<<

Well, I'm giving a talk on the subject to the Tennessee Society for
Histotechnology meeting in Chattanooga, and I'm pretty confused about
the question of who's allowed to gross - the rules seem to be
changing, and different certifying organizations have different
requirements. If somebody can spell out in detail who it is that's
requiring what, I'd appreciate it.

Bob Richmond
Samurai Pathologist
Knoxville TN

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From Herrick.James <@t> mayo.edu  Mon May 17 15:00:13 2010
From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim))
Date: Mon May 17 15:00:20 2010
Subject: FW: [Histonet] Mounting Medium Issues
Message-ID: <7267A64D75F58241B577876D8A885631015A9AE7@msgebe41>



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick,
James L. (Jim)
Sent: Friday, May 14, 2010 3:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium Issues

Hello everyone,

Hope you have all had a good week!!

Has anyone had any negative experiences using Eukitt as your mounting
media? We embed our specimens (human - iliac crest, animal - femurs,
tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period
of time that a fairly large number of our slides are beginning to show
signs of the medium breaking up below the cover slip. It looks as if
there are large air pockets left behind. We are not yet sure why this is
happening. Would any of you geniuses have a resolution for us or know of
a better mounting media to use with plastic embedded specimens -
brightfield and fluorescence? Thanks for your expertise. It is very much
appreciated.

Have a great weekend!!

Jim


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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From jcampbell <@t> vdxpathology.com  Mon May 17 15:12:32 2010
From: jcampbell <@t> vdxpathology.com (Jennifer Campbell)
Date: Mon May 17 15:12:36 2010
Subject: [Histonet] Moving across the country...
Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF83872A7@VDXSERVER01.vdxpathology.local>

Hi All,
  My husband and I will be moving from California to the east coast this
late summer/early fall for his work.....exciting but, a little scary!  I
have started browsing possible histotech jobs available in either NY, CT
or NJ but, its a rather daunting task when you're not familiar with the
area or the labs out there!  And to add to the stress I am also
currently studying for my HT certification which I will be taking in
august....which I will hopefully pass so that I may have a little more
luck job searching!  Does anyone have any advice for me as to where I
could look into or know of any possibilities out there?
Thanks for any advice you may have.
 
Jennifer Campbell 
VDx Veterinary Diagnostics
Davis, CA
phone: (530) 753-4285
fax: (530) 753-4286
From Maria.Katleba <@t> stjoe.org  Mon May 17 15:40:51 2010
From: Maria.Katleba <@t> stjoe.org (Maria Katleba)
Date: Mon May 17 15:41:00 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: 
Message-ID: 

Anyone know what the rules are for California?

Maria Katleba HT(ASCP), MS
Pathology Dept. Mgr.
Queen of the Valley Medical Center
707-294-9229 cell
707-252-4411 x3689

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org
Sent: Monday, May 17, 2010 12:56 PM
To: Robert Richmond
Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Grossing Technician Qualifications

There has been a lot of confusion about this. From my understanding the
real definition of who can gross falls under the "High Complexity" testing
category. This would be different for different states I think as well.
Let me give an example.

In the State of Florida pervious technicians may have been trained to
Gross large specimens( or biopsies ). With this new law a technician in
the state of Florida would not be able to continue to gross because in
actuality they never held a license that allowed them to perform "High
Complexity" testing. So they are not grandfathered in because of that
clause. Technologist would, because they have always been able to preform
"High Complexity" testing.

I also think that which ever laws your state goes by for people who can
perform "High Complexity" testing is your answer.

I hope this helps.



Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



Robert Richmond 
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/15/2010 04:46 PM

To
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] Re: Grossing Technician Qualifications






Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is
strictly limited to grossing
anatomic pathology specimens different than for those of a full time
histotech? Could a bachelor degree'd person with the right course work
qualify for this position? If so, does anyone have documentation of
this? I'd like to present this as a staffing option to my management
if possible.<<

Well, I'm giving a talk on the subject to the Tennessee Society for
Histotechnology meeting in Chattanooga, and I'm pretty confused about
the question of who's allowed to gross - the rules seem to be
changing, and different certifying organizations have different
requirements. If somebody can spell out in detail who it is that's
requiring what, I'd appreciate it.

Bob Richmond
Samurai Pathologist
Knoxville TN

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From mucram11 <@t> comcast.net  Mon May 17 16:21:05 2010
From: mucram11 <@t> comcast.net (Pamela Marcum)
Date: Mon May 17 16:21:09 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: 
Message-ID: <637385080.24337.1274131265435.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>



I went to the American Association of Pathology Assistants web site and then called them.? I got the most help for the question of who can and who can not gross from them.? It will not be state specific so if you think your state has other more complex rules you will need to check there.? The rule I have now are what CAP will look for and that is what I am working toward.? CAP is who will grade us -?not the state overall. 



Pam 





----- Original Message ----- 
From: "Kim Donadio"  
To: "Robert Richmond"  
Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu 
Sent: Monday, May 17, 2010 2:55:33 PM 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications 

There has been a lot of confusion about this. From my understanding the 
real definition of who can gross falls under the "High Complexity" testing 
category. This would be different for different states I think as well. 
Let me give an example. 

In the State of Florida pervious technicians may have been trained to 
Gross large specimens( or biopsies ). With this new law a technician in 
the state of Florida would not be able to continue to gross because in 
actuality they never held a license that allowed them to perform "High 
Complexity" testing. So they are not grandfathered in because of that 
clause. Technologist would, because they have always been able to preform 
"High Complexity" testing. 

I also think that which ever laws your state goes by for people who can 
perform "High Complexity" testing is your answer. 

I hope this helps. 



Kim Donadio 
Pathology Supervisor 
Baptist Hospital 
1000 W Moreno St. 
Pensacola FL 32501 
Phone (850) 469-7718 
Fax (850) 434-4996 



Robert Richmond  
Sent by: histonet-bounces@lists.utsouthwestern.edu 
05/15/2010 04:46 PM 

To 
histonet@lists.utsouthwestern.edu 
cc 

Subject 
[Histonet] Re: Grossing Technician Qualifications 






Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is 
strictly limited to grossing 
anatomic pathology specimens different than for those of a full time 
histotech? Could a bachelor degree'd person with the right course work 
qualify for this position? If so, does anyone have documentation of 
this? I'd like to present this as a staffing option to my management 
if possible.<< 

Well, I'm giving a talk on the subject to the Tennessee Society for 
Histotechnology meeting in Chattanooga, and I'm pretty confused about 
the question of who's allowed to gross - the rules seem to be 
changing, and different certifying organizations have different 
requirements. If somebody can spell out in detail who it is that's 
requiring what, I'd appreciate it. 

Bob Richmond 
Samurai Pathologist 
Knoxville TN 

_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



----------------------------------------- 
All electronic data transmissions originating from or sent to 
Baptist Health Care Corporation (BHC) are subject to monitoring. 
This message along with any attached data, are the confidential and 
proprietary communications of BHC and are intended to be received 
only by the individual or individuals to whom the message has been 
addressed. If the reader of this message is not the intended 
recipient, please take notice that any use, copying, printing, 
forwarding or distribution of this message, in any form, is 
strictly prohibited and may violate State or Federal Law. If you 
have received this transmission in error, please delete or destroy 
all copies of this message. ?For questions, contact the BHC Privacy 
Officer at (850) 434-4472. ?Rev.10/07. 
_______________________________________________ 
Histonet mailing list 
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet 






----- Original Message ----- 
From: "Kim Donadio"  
To: "Robert Richmond"  
Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu 
Sent: Monday, May 17, 2010 2:55:33 PM 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications 

There has been a lot of confusion about this. From my understanding the 
real definition of who can gross falls under the "High Complexity" testing 
category. This would be different for different states I think as well. 
Let me give an example. 

In the State of Florida pervious technicians may have been trained to 
Gross large specimens( or biopsies ). With this new law a technician in 
the state of Florida would not be able to continue to gross because in 
actuality they never held a license that allowed them to perform "High 
Complexity" testing. So they are not grandfathered in because of that 
clause. Technologist would, because they have always been able to preform 
"High Complexity" testing. 

I also think that which ever laws your state goes by for people who can 
perform "High Complexity" testing is your answer. 

I hope this helps. 



Kim Donadio 
Pathology Supervisor 
Baptist Hospital 
1000 W Moreno St. 
Pensacola FL 32501 
Phone (850) 469-7718 
Fax (850) 434-4996 



Robert Richmond  
Sent by: histonet-bounces@lists.utsouthwestern.edu 
05/15/2010 04:46 PM 

To 
histonet@lists.utsouthwestern.edu 
cc 

Subject 
[Histonet] Re: Grossing Technician Qualifications 






Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is 
strictly limited to grossing 
anatomic pathology specimens different than for those of a full time 
histotech? Could a bachelor degree'd person with the right course work 
qualify for this position? If so, does anyone have documentation of 
this? I'd like to present this as a staffing option to my management 
if possible.<< 

Well, I'm giving a talk on the subject to the Tennessee Society for 
Histotechnology meeting in Chattanooga, and I'm pretty confused about 
the question of who's allowed to gross - the rules seem to be 
changing, and different certifying organizations have different 
requirements. If somebody can spell out in detail who it is that's 
requiring what, I'd appreciate it. 

Bob Richmond 
Samurai Pathologist 
Knoxville TN 

_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



----------------------------------------- 
All electronic data transmissions originating from or sent to 
Baptist Health Care Corporation (BHC) are subject to monitoring. 
This message along with any attached data, are the confidential and 
proprietary communications of BHC and are intended to be received 
only by the individual or individuals to whom the message has been 
addressed. If the reader of this message is not the intended 
recipient, please take notice that any use, copying, printing, 
forwarding or distribution of this message, in any form, is 
strictly prohibited and may violate State or Federal Law. If you 
have received this transmission in error, please delete or destroy 
all copies of this message. ?For questions, contact the BHC Privacy 
Officer at (850) 434-4472. ?Rev.10/07. 
_______________________________________________ 
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From tahseen <@t> brain.net.pk  Mon May 17 23:20:43 2010
From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk)
Date: Mon May 17 23:20:51 2010
Subject: [Histonet] carbonic anhydrase 9
Message-ID: <58269.202.125.145.178.1274156443.squirrel@brain.net.pk>

Dear all
We are locking immuno marker for carbonic anhydrase 9 on p.section for
renal cell carcinoma.
Thanks advance
Muhammad Tahseen
Histology Supervisor
SKMCH&RC
Lahore Pakistan





From rjr6 <@t> psu.edu  Tue May 18 07:42:30 2010
From: rjr6 <@t> psu.edu (Roberta Horner)
Date: Tue May 18 07:42:53 2010
Subject: [Histonet] Mycoplasma
Message-ID: 

Does anyone know of a special stain for mycoplasma?  I searched all my books and can't find anything.
Thank you
Roberta Horner HT/HTL
Animal Diagnostic Lab
Penn  State University
From alyssa <@t> alliedsearchpartners.com  Tue May 18 08:03:14 2010
From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson)
Date: Tue May 18 08:03:21 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: <637385080.24337.1274131265435.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>
References: 
	<637385080.24337.1274131265435.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>
Message-ID: 

>From my understanding, what I have learned throughout my recruiting career
is that in order to gross you have to have 60 credit hours of COLLEGE
SCIENCE classes, not necessarily a degree, but definitely the 60 credit
hours at least. This requirement is a nationwide requirement from what I
know, and does not vary from state to state.


On Mon, May 17, 2010 at 5:21 PM, Pamela Marcum  wrote:

>
>
> I went to the American Association of Pathology Assistants web site and
> then called them.  I got the most help for the question of who can and who
> can not gross from them.  It will not be state specific so if you think your
> state has other more complex rules you will need to check there.  The rule I
> have now are what CAP will look for and that is what I am working toward.
> CAP is who will grade us - not the state overall.
>
>
>
> Pam
>
>
>
>
>
> ----- Original Message -----
> From: "Kim Donadio" 
> To: "Robert Richmond" 
> Cc: histonet@lists.utsouthwestern.edu,
> histonet-bounces@lists.utsouthwestern.edu
> Sent: Monday, May 17, 2010 2:55:33 PM
> Subject: Re: [Histonet] Re: Grossing Technician Qualifications
>
> There has been a lot of confusion about this. From my understanding the
> real definition of who can gross falls under the "High Complexity" testing
> category. This would be different for different states I think as well.
> Let me give an example.
>
> In the State of Florida pervious technicians may have been trained to
> Gross large specimens( or biopsies ). With this new law a technician in
> the state of Florida would not be able to continue to gross because in
> actuality they never held a license that allowed them to perform "High
> Complexity" testing. So they are not grandfathered in because of that
> clause. Technologist would, because they have always been able to preform
> "High Complexity" testing.
>
> I also think that which ever laws your state goes by for people who can
> perform "High Complexity" testing is your answer.
>
> I hope this helps.
>
>
>
> Kim Donadio
> Pathology Supervisor
> Baptist Hospital
> 1000 W Moreno St.
> Pensacola FL 32501
> Phone (850) 469-7718
> Fax (850) 434-4996
>
>
>
> Robert Richmond 
> Sent by: histonet-bounces@lists.utsouthwestern.edu
> 05/15/2010 04:46 PM
>
> To
> histonet@lists.utsouthwestern.edu
> cc
>
> Subject
> [Histonet] Re: Grossing Technician Qualifications
>
>
>
>
>
>
> Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is
> strictly limited to grossing
> anatomic pathology specimens different than for those of a full time
> histotech? Could a bachelor degree'd person with the right course work
> qualify for this position? If so, does anyone have documentation of
> this? I'd like to present this as a staffing option to my management
> if possible.<<
>
> Well, I'm giving a talk on the subject to the Tennessee Society for
> Histotechnology meeting in Chattanooga, and I'm pretty confused about
> the question of who's allowed to gross - the rules seem to be
> changing, and different certifying organizations have different
> requirements. If somebody can spell out in detail who it is that's
> requiring what, I'd appreciate it.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> -----------------------------------------
> All electronic data transmissions originating from or sent to
> Baptist Health Care Corporation (BHC) are subject to monitoring.
> This message along with any attached data, are the confidential and
> proprietary communications of BHC and are intended to be received
> only by the individual or individuals to whom the message has been
> addressed. If the reader of this message is not the intended
> recipient, please take notice that any use, copying, printing,
> forwarding or distribution of this message, in any form, is
> strictly prohibited and may violate State or Federal Law. If you
> have received this transmission in error, please delete or destroy
> all copies of this message.  For questions, contact the BHC Privacy
> Officer at (850) 434-4472.  Rev.10/07.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>  ----- Original Message -----
> From: "Kim Donadio" 
> To: "Robert Richmond" 
> Cc: histonet@lists.utsouthwestern.edu,
> histonet-bounces@lists.utsouthwestern.edu
> Sent: Monday, May 17, 2010 2:55:33 PM
> Subject: Re: [Histonet] Re: Grossing Technician Qualifications
>
> There has been a lot of confusion about this. From my understanding the
> real definition of who can gross falls under the "High Complexity" testing
> category. This would be different for different states I think as well.
> Let me give an example.
>
> In the State of Florida pervious technicians may have been trained to
> Gross large specimens( or biopsies ). With this new law a technician in
> the state of Florida would not be able to continue to gross because in
> actuality they never held a license that allowed them to perform "High
> Complexity" testing. So they are not grandfathered in because of that
> clause. Technologist would, because they have always been able to preform
> "High Complexity" testing.
>
> I also think that which ever laws your state goes by for people who can
> perform "High Complexity" testing is your answer.
>
> I hope this helps.
>
>
>
> Kim Donadio
> Pathology Supervisor
> Baptist Hospital
> 1000 W Moreno St.
> Pensacola FL 32501
> Phone (850) 469-7718
> Fax (850) 434-4996
>
>
>
> Robert Richmond 
> Sent by: histonet-bounces@lists.utsouthwestern.edu
> 05/15/2010 04:46 PM
>
> To
> histonet@lists.utsouthwestern.edu
> cc
>
> Subject
> [Histonet] Re: Grossing Technician Qualifications
>
>
>
>
>
>
> Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is
> strictly limited to grossing
> anatomic pathology specimens different than for those of a full time
> histotech? Could a bachelor degree'd person with the right course work
> qualify for this position? If so, does anyone have documentation of
> this? I'd like to present this as a staffing option to my management
> if possible.<<
>
> Well, I'm giving a talk on the subject to the Tennessee Society for
> Histotechnology meeting in Chattanooga, and I'm pretty confused about
> the question of who's allowed to gross - the rules seem to be
> changing, and different certifying organizations have different
> requirements. If somebody can spell out in detail who it is that's
> requiring what, I'd appreciate it.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> -----------------------------------------
> All electronic data transmissions originating from or sent to
> Baptist Health Care Corporation (BHC) are subject to monitoring.
> This message along with any attached data, are the confidential and
> proprietary communications of BHC and are intended to be received
> only by the individual or individuals to whom the message has been
> addressed. If the reader of this message is not the intended
> recipient, please take notice that any use, copying, printing,
> forwarding or distribution of this message, in any form, is
> strictly prohibited and may violate State or Federal Law. If you
> have received this transmission in error, please delete or destroy
> all copies of this message.  For questions, contact the BHC Privacy
> Officer at (850) 434-4472.  Rev.10/07.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From mward <@t> wfubmc.edu  Tue May 18 08:07:21 2010
From: mward <@t> wfubmc.edu (Martha Ward)
Date: Tue May 18 08:07:27 2010
Subject: [Histonet] p63 antibody
Message-ID: <61135F0455D33347B5AAE209B903A30433DEB970@EXCHVS2.medctr.ad.wfubmc.edu>

This may have been discussed before but....now that Dako is no longer
offering p63, which vendor are most of you switching to?   Thanks in
advance for your help.
 
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
336-716-2104
 
 
 
From pruegg <@t> ihctech.net  Tue May 18 08:29:18 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Tue May 18 08:30:06 2010
Subject: [Histonet] Moving across the country...
In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF83872A7@VDXSERVER01.vdxpathology.local>
References: <5658CBDB9EAE6545ABE50D2563D81BF83872A7@VDXSERVER01.vdxpathology.local>
Message-ID: <14908CEF585B4206BECEF866C5726324@prueggihctechlt>

Jennifer,

You should try some of the job placement agencies, they are always looking
for techs to fill jobs and there is no cost to you.  I bet you will be
contacted just from placing this post on Histonet.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer
Campbell
Sent: Monday, May 17, 2010 2:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Moving across the country...

Hi All,
  My husband and I will be moving from California to the east coast this
late summer/early fall for his work.....exciting but, a little scary!  I
have started browsing possible histotech jobs available in either NY, CT
or NJ but, its a rather daunting task when you're not familiar with the
area or the labs out there!  And to add to the stress I am also
currently studying for my HT certification which I will be taking in
august....which I will hopefully pass so that I may have a little more
luck job searching!  Does anyone have any advice for me as to where I
could look into or know of any possibilities out there?
Thanks for any advice you may have.
 
Jennifer Campbell 
VDx Veterinary Diagnostics
Davis, CA
phone: (530) 753-4285
fax: (530) 753-4286
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From Ronald.Houston <@t> nationwidechildrens.org  Tue May 18 08:41:59 2010
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Tue May 18 08:42:36 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: <637385080.24337.1274131265435.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>
References: 
	<637385080.24337.1274131265435.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>
Message-ID: <979FF5962E234F45B06CF0DB7C1AABB22669E7A7@chi2k3ms01.columbuschildrens.net>

Care to share with us what they told you regarding the specifics of who can and who cannot gross?

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum
Sent: Monday, May 17, 2010 5:21 PM
To: Kim Donadio
Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Robert Richmond
Subject: Re: [Histonet] Re: Grossing Technician Qualifications



I went to the American Association of Pathology Assistants web site and then called them.? I got the most help for the question of who can and who can not gross from them.? It will not be state specific so if you think your state has other more complex rules you will need to check there.? The rule I have now are what CAP will look for and that is what I am working toward.? CAP is who will grade us -?not the state overall. 



Pam 





----- Original Message ----- 
From: "Kim Donadio"  
To: "Robert Richmond"  
Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu 
Sent: Monday, May 17, 2010 2:55:33 PM 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications 

There has been a lot of confusion about this. From my understanding the 
real definition of who can gross falls under the "High Complexity" testing 
category. This would be different for different states I think as well. 
Let me give an example. 

In the State of Florida pervious technicians may have been trained to 
Gross large specimens( or biopsies ). With this new law a technician in 
the state of Florida would not be able to continue to gross because in 
actuality they never held a license that allowed them to perform "High 
Complexity" testing. So they are not grandfathered in because of that 
clause. Technologist would, because they have always been able to preform 
"High Complexity" testing. 

I also think that which ever laws your state goes by for people who can 
perform "High Complexity" testing is your answer. 

I hope this helps. 



Kim Donadio 
Pathology Supervisor 
Baptist Hospital 
1000 W Moreno St. 
Pensacola FL 32501 
Phone (850) 469-7718 
Fax (850) 434-4996 



Robert Richmond  
Sent by: histonet-bounces@lists.utsouthwestern.edu 
05/15/2010 04:46 PM 

To 
histonet@lists.utsouthwestern.edu 
cc 

Subject 
[Histonet] Re: Grossing Technician Qualifications 






Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is 
strictly limited to grossing 
anatomic pathology specimens different than for those of a full time 
histotech? Could a bachelor degree'd person with the right course work 
qualify for this position? If so, does anyone have documentation of 
this? I'd like to present this as a staffing option to my management 
if possible.<< 

Well, I'm giving a talk on the subject to the Tennessee Society for 
Histotechnology meeting in Chattanooga, and I'm pretty confused about 
the question of who's allowed to gross - the rules seem to be 
changing, and different certifying organizations have different 
requirements. If somebody can spell out in detail who it is that's 
requiring what, I'd appreciate it. 

Bob Richmond 
Samurai Pathologist 
Knoxville TN 

_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



----------------------------------------- 
All electronic data transmissions originating from or sent to 
Baptist Health Care Corporation (BHC) are subject to monitoring. 
This message along with any attached data, are the confidential and 
proprietary communications of BHC and are intended to be received 
only by the individual or individuals to whom the message has been 
addressed. If the reader of this message is not the intended 
recipient, please take notice that any use, copying, printing, 
forwarding or distribution of this message, in any form, is 
strictly prohibited and may violate State or Federal Law. If you 
have received this transmission in error, please delete or destroy 
all copies of this message. ?For questions, contact the BHC Privacy 
Officer at (850) 434-4472. ?Rev.10/07. 
_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 






----- Original Message ----- 
From: "Kim Donadio"  
To: "Robert Richmond"  
Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu 
Sent: Monday, May 17, 2010 2:55:33 PM 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications 

There has been a lot of confusion about this. From my understanding the 
real definition of who can gross falls under the "High Complexity" testing 
category. This would be different for different states I think as well. 
Let me give an example. 

In the State of Florida pervious technicians may have been trained to 
Gross large specimens( or biopsies ). With this new law a technician in 
the state of Florida would not be able to continue to gross because in 
actuality they never held a license that allowed them to perform "High 
Complexity" testing. So they are not grandfathered in because of that 
clause. Technologist would, because they have always been able to preform 
"High Complexity" testing. 

I also think that which ever laws your state goes by for people who can 
perform "High Complexity" testing is your answer. 

I hope this helps. 



Kim Donadio 
Pathology Supervisor 
Baptist Hospital 
1000 W Moreno St. 
Pensacola FL 32501 
Phone (850) 469-7718 
Fax (850) 434-4996 



Robert Richmond  
Sent by: histonet-bounces@lists.utsouthwestern.edu 
05/15/2010 04:46 PM 

To 
histonet@lists.utsouthwestern.edu 
cc 

Subject 
[Histonet] Re: Grossing Technician Qualifications 






Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is 
strictly limited to grossing 
anatomic pathology specimens different than for those of a full time 
histotech? Could a bachelor degree'd person with the right course work 
qualify for this position? If so, does anyone have documentation of 
this? I'd like to present this as a staffing option to my management 
if possible.<< 

Well, I'm giving a talk on the subject to the Tennessee Society for 
Histotechnology meeting in Chattanooga, and I'm pretty confused about 
the question of who's allowed to gross - the rules seem to be 
changing, and different certifying organizations have different 
requirements. If somebody can spell out in detail who it is that's 
requiring what, I'd appreciate it. 

Bob Richmond 
Samurai Pathologist 
Knoxville TN 

_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



----------------------------------------- 
All electronic data transmissions originating from or sent to 
Baptist Health Care Corporation (BHC) are subject to monitoring. 
This message along with any attached data, are the confidential and 
proprietary communications of BHC and are intended to be received 
only by the individual or individuals to whom the message has been 
addressed. If the reader of this message is not the intended 
recipient, please take notice that any use, copying, printing, 
forwarding or distribution of this message, in any form, is 
strictly prohibited and may violate State or Federal Law. If you 
have received this transmission in error, please delete or destroy 
all copies of this message. ?For questions, contact the BHC Privacy 
Officer at (850) 434-4472. ?Rev.10/07. 
_______________________________________________ 
Histonet mailing list 
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----------------------------------------- Confidentiality Notice:
The following mail message, including any attachments, is for the
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and privileged information. The recipient is responsible to
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reliance on the contents of this e-mail is strictly prohibited. If
you have received this communication in error, please notify us
immediately by reply e-mail and destroy all copies of the original
message. Thank you.

From rjbuesa <@t> yahoo.com  Tue May 18 08:45:01 2010
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue May 18 08:45:11 2010
Subject: [Histonet] Mycoplasma
In-Reply-To: 
Message-ID: <562038.42421.qm@web65702.mail.ac4.yahoo.com>

Roberta:
Sigma-Aldrich offer a staining kit for Mycoplasma.
Their cat. # MYC1
Ren? J.

--- On Tue, 5/18/10, Roberta Horner  wrote:


From: Roberta Horner 
Subject: [Histonet] Mycoplasma
To: "histonet@lists.utsouthwestern.edu" 
Date: Tuesday, May 18, 2010, 8:42 AM


Does anyone know of a special stain for mycoplasma?? I searched all my books and can't find anything.
Thank you
Roberta Horner HT/HTL
Animal Diagnostic Lab
Penn? State University
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From LSebree <@t> uwhealth.org  Tue May 18 08:47:38 2010
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Tue May 18 08:47:48 2010
Subject: [Histonet] p63 antibody
In-Reply-To: <61135F0455D33347B5AAE209B903A30433DEB970@EXCHVS2.medctr.ad.wfubmc.edu>
References: <61135F0455D33347B5AAE209B903A30433DEB970@EXCHVS2.medctr.ad.wfubmc.edu>
Message-ID: <8C023B4AB999614BA4791BAEB26E2738399E5F@UWHC-MAIL01.uwhis.hosp.wisc.edu>

We use Biocare's, clone BC4A4. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha
Ward
Sent: Tuesday, May 18, 2010 8:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p63 antibody

This may have been discussed before but....now that Dako is no longer
offering p63, which vendor are most of you switching to?   Thanks in
advance for your help.
 
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab Wake Forest University
Baptist Medical Center
336-716-2104
 
 
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From alyssa <@t> alliedsearchpartners.com  Tue May 18 08:49:24 2010
From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson)
Date: Tue May 18 08:49:36 2010
Subject: [Histonet] Histotech Needed In FL
Message-ID: 

Our client, located in Plant City, FL, has retained Allied Search Partners
and MPath Search Partners to assist in the search for a highly qualified
Histotechnician/Histotechnologist.   This is a unique opportunity to join a
financially strong and growing organization in a key role within the
company.



Shift: Full Time/Permanent, 3am-11am



Requirements: Florida Licensed Technician or Technologist, ASCP preferred



To apply: Please submit resume to Alyssa@alliedsearchpartners.com



*No resume will be sent to our client until we speak with you, all resumes
are kept confidential*
From andreas.kappeler <@t> pathology.unibe.ch  Tue May 18 09:05:30 2010
From: andreas.kappeler <@t> pathology.unibe.ch (Kappeler, Andreas (PATHOLOGY))
Date: Tue May 18 09:08:18 2010
Subject: [Histonet] AW: p63 antibody
In-Reply-To: <61135F0455D33347B5AAE209B903A30433DEB970@EXCHVS2.medctr.ad.wfubmc.edu>
References: <61135F0455D33347B5AAE209B903A30433DEB970@EXCHVS2.medctr.ad.wfubmc.edu>
Message-ID: <4EAFACC375BF974C8A57D33444CEE288049AEFDCF0@AAI-MBX3.campus.unibe.ch>

We have ours from Thermo Scientific, CatNo MS-1081, same clone (4A4), works very well (dilution 1:400 in our hands).
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

________________________________________
Von: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] im Auftrag von Martha Ward [mward@wfubmc.edu]
Gesendet: Dienstag, 18. Mai 2010 15:07
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] p63 antibody

This may have been discussed before but....now that Dako is no longer
offering p63, which vendor are most of you switching to?   Thanks in
advance for your help.

Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
336-716-2104



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From mucram11 <@t> comcast.net  Tue May 18 09:42:45 2010
From: mucram11 <@t> comcast.net (Pamela Marcum)
Date: Tue May 18 09:43:02 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB22669E7A7@chi2k3ms01.columbuschildrens.net>
Message-ID: <1704014936.47242.1274193765256.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>



It is too long to type and can be found in the April 7th, AAPA e-news Today or it is ANP.11610 Phase II CAP regualtions/Exceptions Grandfathered at 42CFR493.1489 and 1491.? These are the exact regulations and should anwser all questions about who can and a cannot gross.? Sorry I just don't have it in way to send easily.? I contacted the AAPA and got the info. 



Pam Marcum 

Anatomic Pathology Manager 

UAMS 



----- Original Message ----- 
From: "Ronald Houston"  
To: "Pamela Marcum" , "Kim Donadio"  
Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu, "Robert Richmond"  
Sent: Tuesday, May 18, 2010 8:41:59 AM 
Subject: RE: [Histonet] Re: Grossing Technician Qualifications 

Care to share with us what they told you regarding the specifics of who can and who cannot gross? 

Ronnie Houston 
Anatomic Pathology Manager 
Nationwide Children's Hospital 
Columbus OH 43205 
(614) 722 5450 
-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum 
Sent: Monday, May 17, 2010 5:21 PM 
To: Kim Donadio 
Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Robert Richmond 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications 



I went to the American Association of Pathology Assistants web site and then called them.? I got the most help for the question of who can and who can not gross from them.? It will not be state specific so if you think your state has other more complex rules you will need to check there.? The rule I have now are what CAP will look for and that is what I am working toward.? CAP is who will grade us -?not the state overall. 



Pam 





----- Original Message ----- 
From: "Kim Donadio"  
To: "Robert Richmond"  
Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu 
Sent: Monday, May 17, 2010 2:55:33 PM 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications 

There has been a lot of confusion about this. From my understanding the 
real definition of who can gross falls under the "High Complexity" testing 
category. This would be different for different states I think as well. 
Let me give an example. 

In the State of Florida pervious technicians may have been trained to 
Gross large specimens( or biopsies ). With this new law a technician in 
the state of Florida would not be able to continue to gross because in 
actuality they never held a license that allowed them to perform "High 
Complexity" testing. So they are not grandfathered in because of that 
clause. Technologist would, because they have always been able to preform 
"High Complexity" testing. 

I also think that which ever laws your state goes by for people who can 
perform "High Complexity" testing is your answer. 

I hope this helps. 



Kim Donadio 
Pathology Supervisor 
Baptist Hospital 
1000 W Moreno St. 
Pensacola FL 32501 
Phone (850) 469-7718 
Fax (850) 434-4996 



Robert Richmond  
Sent by: histonet-bounces@lists.utsouthwestern.edu 
05/15/2010 04:46 PM 

To 
histonet@lists.utsouthwestern.edu 
cc 

Subject 
[Histonet] Re: Grossing Technician Qualifications 






Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is 
strictly limited to grossing 
anatomic pathology specimens different than for those of a full time 
histotech? Could a bachelor degree'd person with the right course work 
qualify for this position? If so, does anyone have documentation of 
this? I'd like to present this as a staffing option to my management 
if possible.<< 

Well, I'm giving a talk on the subject to the Tennessee Society for 
Histotechnology meeting in Chattanooga, and I'm pretty confused about 
the question of who's allowed to gross - the rules seem to be 
changing, and different certifying organizations have different 
requirements. If somebody can spell out in detail who it is that's 
requiring what, I'd appreciate it. 

Bob Richmond 
Samurai Pathologist 
Knoxville TN 

_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



----------------------------------------- 
All electronic data transmissions originating from or sent to 
Baptist Health Care Corporation (BHC) are subject to monitoring. 
This message along with any attached data, are the confidential and 
proprietary communications of BHC and are intended to be received 
only by the individual or individuals to whom the message has been 
addressed. If the reader of this message is not the intended 
recipient, please take notice that any use, copying, printing, 
forwarding or distribution of this message, in any form, is 
strictly prohibited and may violate State or Federal Law. If you 
have received this transmission in error, please delete or destroy 
all copies of this message. ?For questions, contact the BHC Privacy 
Officer at (850) 434-4472. ?Rev.10/07. 
_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 






----- Original Message ----- 
From: "Kim Donadio"  
To: "Robert Richmond"  
Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu 
Sent: Monday, May 17, 2010 2:55:33 PM 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications 

There has been a lot of confusion about this. From my understanding the 
real definition of who can gross falls under the "High Complexity" testing 
category. This would be different for different states I think as well. 
Let me give an example. 

In the State of Florida pervious technicians may have been trained to 
Gross large specimens( or biopsies ). With this new law a technician in 
the state of Florida would not be able to continue to gross because in 
actuality they never held a license that allowed them to perform "High 
Complexity" testing. So they are not grandfathered in because of that 
clause. Technologist would, because they have always been able to preform 
"High Complexity" testing. 

I also think that which ever laws your state goes by for people who can 
perform "High Complexity" testing is your answer. 

I hope this helps. 



Kim Donadio 
Pathology Supervisor 
Baptist Hospital 
1000 W Moreno St. 
Pensacola FL 32501 
Phone (850) 469-7718 
Fax (850) 434-4996 



Robert Richmond  
Sent by: histonet-bounces@lists.utsouthwestern.edu 
05/15/2010 04:46 PM 

To 
histonet@lists.utsouthwestern.edu 
cc 

Subject 
[Histonet] Re: Grossing Technician Qualifications 






Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is 
strictly limited to grossing 
anatomic pathology specimens different than for those of a full time 
histotech? Could a bachelor degree'd person with the right course work 
qualify for this position? If so, does anyone have documentation of 
this? I'd like to present this as a staffing option to my management 
if possible.<< 

Well, I'm giving a talk on the subject to the Tennessee Society for 
Histotechnology meeting in Chattanooga, and I'm pretty confused about 
the question of who's allowed to gross - the rules seem to be 
changing, and different certifying organizations have different 
requirements. If somebody can spell out in detail who it is that's 
requiring what, I'd appreciate it. 

Bob Richmond 
Samurai Pathologist 
Knoxville TN 

_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



----------------------------------------- 
All electronic data transmissions originating from or sent to 
Baptist Health Care Corporation (BHC) are subject to monitoring. 
This message along with any attached data, are the confidential and 
proprietary communications of BHC and are intended to be received 
only by the individual or individuals to whom the message has been 
addressed. If the reader of this message is not the intended 
recipient, please take notice that any use, copying, printing, 
forwarding or distribution of this message, in any form, is 
strictly prohibited and may violate State or Federal Law. If you 
have received this transmission in error, please delete or destroy 
all copies of this message. ?For questions, contact the BHC Privacy 
Officer at (850) 434-4472. ?Rev.10/07. 
_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
----------------------------------------- Confidentiality Notice: 
The following mail message, including any attachments, is for the 
sole use of the intended recipient(s) and may contain confidential 
and privileged information. The recipient is responsible to 
maintain the confidentiality of this information and to use the 
information only for authorized purposes. If you are not the 
intended recipient (or authorized to receive information for the 
intended recipient), you are hereby notified that any review, use, 
disclosure, distribution, copying, printing, or action taken in 
reliance on the contents of this e-mail is strictly prohibited. If 
you have received this communication in error, please notify us 
immediately by reply e-mail and destroy all copies of the original 
message. Thank you. 


UAMS 



----- Original Message ----- 
From: "Ronald Houston"  
To: "Pamela Marcum" , "Kim Donadio"  
Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu, "Robert Richmond"  
Sent: Tuesday, May 18, 2010 8:41:59 AM 
Subject: RE: [Histonet] Re: Grossing Technician Qualifications 

Care to share with us what they told you regarding the specifics of who can and who cannot gross? 

Ronnie Houston 
Anatomic Pathology Manager 
Nationwide Children's Hospital 
Columbus OH 43205 
(614) 722 5450 
-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum 
Sent: Monday, May 17, 2010 5:21 PM 
To: Kim Donadio 
Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Robert Richmond 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications 



I went to the American Association of Pathology Assistants web site and then called them.? I got the most help for the question of who can and who can not gross from them.? It will not be state specific so if you think your state has other more complex rules you will need to check there.? The rule I have now are what CAP will look for and that is what I am working toward.? CAP is who will grade us -?not the state overall. 



Pam 





----- Original Message ----- 
From: "Kim Donadio"  
To: "Robert Richmond"  
Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu 
Sent: Monday, May 17, 2010 2:55:33 PM 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications 

There has been a lot of confusion about this. From my understanding the 
real definition of who can gross falls under the "High Complexity" testing 
category. This would be different for different states I think as well. 
Let me give an example. 

In the State of Florida pervious technicians may have been trained to 
Gross large specimens( or biopsies ). With this new law a technician in 
the state of Florida would not be able to continue to gross because in 
actuality they never held a license that allowed them to perform "High 
Complexity" testing. So they are not grandfathered in because of that 
clause. Technologist would, because they have always been able to preform 
"High Complexity" testing. 

I also think that which ever laws your state goes by for people who can 
perform "High Complexity" testing is your answer. 

I hope this helps. 



Kim Donadio 
Pathology Supervisor 
Baptist Hospital 
1000 W Moreno St. 
Pensacola FL 32501 
Phone (850) 469-7718 
Fax (850) 434-4996 



Robert Richmond  
Sent by: histonet-bounces@lists.utsouthwestern.edu 
05/15/2010 04:46 PM 

To 
histonet@lists.utsouthwestern.edu 
cc 

Subject 
[Histonet] Re: Grossing Technician Qualifications 






Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is 
strictly limited to grossing 
anatomic pathology specimens different than for those of a full time 
histotech? Could a bachelor degree'd person with the right course work 
qualify for this position? If so, does anyone have documentation of 
this? I'd like to present this as a staffing option to my management 
if possible.<< 

Well, I'm giving a talk on the subject to the Tennessee Society for 
Histotechnology meeting in Chattanooga, and I'm pretty confused about 
the question of who's allowed to gross - the rules seem to be 
changing, and different certifying organizations have different 
requirements. If somebody can spell out in detail who it is that's 
requiring what, I'd appreciate it. 

Bob Richmond 
Samurai Pathologist 
Knoxville TN 

_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



----------------------------------------- 
All electronic data transmissions originating from or sent to 
Baptist Health Care Corporation (BHC) are subject to monitoring. 
This message along with any attached data, are the confidential and 
proprietary communications of BHC and are intended to be received 
only by the individual or individuals to whom the message has been 
addressed. If the reader of this message is not the intended 
recipient, please take notice that any use, copying, printing, 
forwarding or distribution of this message, in any form, is 
strictly prohibited and may violate State or Federal Law. If you 
have received this transmission in error, please delete or destroy 
all copies of this message. ?For questions, contact the BHC Privacy 
Officer at (850) 434-4472. ?Rev.10/07. 
_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 






----- Original Message ----- 
From: "Kim Donadio"  
To: "Robert Richmond"  
Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu 
Sent: Monday, May 17, 2010 2:55:33 PM 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications 

There has been a lot of confusion about this. From my understanding the 
real definition of who can gross falls under the "High Complexity" testing 
category. This would be different for different states I think as well. 
Let me give an example. 

In the State of Florida pervious technicians may have been trained to 
Gross large specimens( or biopsies ). With this new law a technician in 
the state of Florida would not be able to continue to gross because in 
actuality they never held a license that allowed them to perform "High 
Complexity" testing. So they are not grandfathered in because of that 
clause. Technologist would, because they have always been able to preform 
"High Complexity" testing. 

I also think that which ever laws your state goes by for people who can 
perform "High Complexity" testing is your answer. 

I hope this helps. 



Kim Donadio 
Pathology Supervisor 
Baptist Hospital 
1000 W Moreno St. 
Pensacola FL 32501 
Phone (850) 469-7718 
Fax (850) 434-4996 



Robert Richmond  
Sent by: histonet-bounces@lists.utsouthwestern.edu 
05/15/2010 04:46 PM 

To 
histonet@lists.utsouthwestern.edu 
cc 

Subject 
[Histonet] Re: Grossing Technician Qualifications 






Tanisha Neely HT(ASCP) asks: >>Are the guidelines for a tech who is 
strictly limited to grossing 
anatomic pathology specimens different than for those of a full time 
histotech? Could a bachelor degree'd person with the right course work 
qualify for this position? If so, does anyone have documentation of 
this? I'd like to present this as a staffing option to my management 
if possible.<< 

Well, I'm giving a talk on the subject to the Tennessee Society for 
Histotechnology meeting in Chattanooga, and I'm pretty confused about 
the question of who's allowed to gross - the rules seem to be 
changing, and different certifying organizations have different 
requirements. If somebody can spell out in detail who it is that's 
requiring what, I'd appreciate it. 

Bob Richmond 
Samurai Pathologist 
Knoxville TN 

_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



----------------------------------------- 
All electronic data transmissions originating from or sent to 
Baptist Health Care Corporation (BHC) are subject to monitoring. 
This message along with any attached data, are the confidential and 
proprietary communications of BHC and are intended to be received 
only by the individual or individuals to whom the message has been 
addressed. If the reader of this message is not the intended 
recipient, please take notice that any use, copying, printing, 
forwarding or distribution of this message, in any form, is 
strictly prohibited and may violate State or Federal Law. If you 
have received this transmission in error, please delete or destroy 
all copies of this message. ?For questions, contact the BHC Privacy 
Officer at (850) 434-4472. ?Rev.10/07. 
_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
----------------------------------------- Confidentiality Notice: 
The following mail message, including any attachments, is for the 
sole use of the intended recipient(s) and may contain confidential 
and privileged information. The recipient is responsible to 
maintain the confidentiality of this information and to use the 
information only for authorized purposes. If you are not the 
intended recipient (or authorized to receive information for the 
intended recipient), you are hereby notified that any review, use, 
disclosure, distribution, copying, printing, or action taken in 
reliance on the contents of this e-mail is strictly prohibited. If 
you have received this communication in error, please notify us 
immediately by reply e-mail and destroy all copies of the original 
message. Thank you. 
From sjkitten <@t> live.com  Tue May 18 09:52:26 2010
From: sjkitten <@t> live.com (S R)
Date: Tue May 18 09:52:57 2010
Subject: [Histonet] Urine Cytology
Message-ID: 


Hello,

My company would like to start doing urine cytologies.  Being a histotech i am not 100% sure as to what i need to do this because i have to learned how to di it yet :-)  Any suggestions would be greatly appreciated!

Thanks in Advanced

S

 
 		 	   		  
_________________________________________________________________
The New Busy is not the old busy. Search, chat and e-mail from your inbox.
http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3
From brandihiggins <@t> gmail.com  Tue May 18 10:30:11 2010
From: brandihiggins <@t> gmail.com (Brandi Higgins)
Date: Tue May 18 10:38:57 2010
Subject: [Histonet] alternative for bunsen burner?
Message-ID: 

Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they don't
have to install a gas line.  The pathologist has told me that there is some
machine that can be used instead of a flame to burn the forceps while
embedding (which is our main use for the bunsen flame).  This is the only
lab I have ever worked at and I don't know what such a machine would be
called.  If anyone knows the name, or any other alternative method can you
let me know.  Model numbers/companies would be a bonus too!  Thanks so much
for your help.

Brandi Higgins, BS, HT(ASCP)
From Bill.Tench <@t> pph.org  Tue May 18 10:54:45 2010
From: Bill.Tench <@t> pph.org (Tench, Bill)
Date: Tue May 18 10:54:57 2010
Subject: [Histonet] Grossing assistants-pathologist assistants
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD028630EB@MAIL1.pph.local>

Yes, the Pathology Assistant national organization is a great resource
as is the CAP.  As I said before, please remember that the CAP
inspection standards represent compliance with FEDERAL CMS standards.  I
would encourage contact with the CAP LAP  in regard to these FEDERAL
standards.  Individual states may also have requirements specific for
that state, which certainly may complicate things, so contact with your
state pathology society will help with that. 

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

Bill.Tench@pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


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From sgoebel <@t> xbiotech.com  Tue May 18 11:03:23 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Tue May 18 11:03:41 2010
Subject: [Histonet] Urine Cytology
Message-ID: <20100518090323.9e2d9aa830e8449a2412eb1e4f2f067e.9ba84e7acf.wbe@email04.secureserver.net>


   The  way  we always did it was to get the specimen fresh (so 24 hou   urine  is usually not a good specimen), then make two cytospins.  Sta   in  one  with  diff quik and one with a PAP stain.  We never made cell
   bl   air d
   Sarah Goebel, B.A., HT (ASCP)
   XBiotech USA Inc.

   
   8201 East
   <
   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] Urine Cytology
   From: S R 
   Date: Tue, May 18, 2010 7:52 am
   To: histo net 
   Hello,
   My  company  would  like  to  start  doing  urine  cytologies. Being a
   histotech  i   have  to  learned  ho   greatly appreciated!
   Thanks in Advanced
   S
   _________________________________________________________________
   The  New  Busy  is not the old busy. Search, chat and e-mail from your
   inbox.<   ?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3_______________
   _____   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
References

   1. 3D"http://www.windowslive.com/campaign/thenewbusy   2. 3D"http://lists.utsouthwestern.edu/mailman/listin
From mpence <@t> grhs.net  Tue May 18 11:11:52 2010
From: mpence <@t> grhs.net (Mike Pence)
Date: Tue May 18 11:11:59 2010
Subject: [Histonet] alternative for bunsen burner?
In-Reply-To: 
Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3E1E@is-e2k3.grhs.net>

Why are you still using a bunsen burner? I believe there is a CAP
question about "No open flames" in the lab. I would suggest finding a
replacement.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi
Higgins
Sent: Tuesday, May 18, 2010 10:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] alternative for bunsen burner?


Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they
don't have to install a gas line.  The pathologist has told me that
there is some machine that can be used instead of a flame to burn the
forceps while embedding (which is our main use for the bunsen flame).
This is the only lab I have ever worked at and I don't know what such a
machine would be called.  If anyone knows the name, or any other
alternative method can you let me know.  Model numbers/companies would
be a bonus too!  Thanks so much for your help.

Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From JMacDonald <@t> mtsac.edu  Tue May 18 11:27:53 2010
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Tue May 18 11:28:01 2010
Subject: [Histonet] alternative for bunsen burner?
In-Reply-To: 
Message-ID: 

a bacti incinerator




Brandi Higgins  
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/18/2010 09:00 AM

To
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] alternative for bunsen burner?






Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they don't
have to install a gas line.  The pathologist has told me that there is 
some
machine that can be used instead of a flame to burn the forceps while
embedding (which is our main use for the bunsen flame).  This is the only
lab I have ever worked at and I don't know what such a machine would be
called.  If anyone knows the name, or any other alternative method can you
let me know.  Model numbers/companies would be a bonus too!  Thanks so 
much
for your help.

Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From BSullivan <@t> shorememorial.org  Tue May 18 11:46:40 2010
From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org)
Date: Tue May 18 11:48:52 2010
Subject: [Histonet] alternative for bunsen burner?
In-Reply-To: 
Message-ID: 

We use an alcohol burner that has a wick inside. It works well for us.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


                                                                           
             Brandi Higgins                                                
                                                                To 
             Sent by:                  histonet@lists.utsouthwestern.edu   
             histonet-bounces@                                          cc 
             lists.utsouthwest                                             
             ern.edu                                               Subject 
                                       [Histonet] alternative for bunsen   
                                       burner?                             
             05/18/2010 11:30                                              
             AM                                                            
                                                                           
                                                                           
                                                                           
                                                                           




Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they don't
have to install a gas line.  The pathologist has told me that there is some
machine that can be used instead of a flame to burn the forceps while
embedding (which is our main use for the bunsen flame).  This is the only
lab I have ever worked at and I don't know what such a machine would be
called.  If anyone knows the name, or any other alternative method can you
let me know.  Model numbers/companies would be a bonus too!  Thanks so much
for your help.

Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From Jackie.O'Connor <@t> abbott.com  Tue May 18 11:52:37 2010
From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor)
Date: Tue May 18 11:53:23 2010
Subject: [Histonet] alternative for bunsen burner?
In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3E1E@is-e2k3.grhs.net>
References: 
	<661949901A768E4F9CC16D8AF8F2838C017A3E1E@is-e2k3.grhs.net>
Message-ID: 

I think that's the crux of her question - what can she use as an 
alternative?   You can find forceps warmers here.   
http://americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=EQFW-120
Although, I still miss the ol' bunsen burner.   It was great for burning 
away little fragments of tissue from between the teeth - of the forceps, 
not my teeth. 



From:
"Mike Pence" 
To:
"Brandi Higgins" , 

Date:
05/18/2010 11:42 AM
Subject:
RE: [Histonet] alternative for bunsen burner?
Sent by:
histonet-bounces@lists.utsouthwestern.edu



Why are you still using a bunsen burner? I believe there is a CAP
question about "No open flames" in the lab. I would suggest finding a
replacement.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi
Higgins
Sent: Tuesday, May 18, 2010 10:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] alternative for bunsen burner?


Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they
don't have to install a gas line.  The pathologist has told me that
there is some machine that can be used instead of a flame to burn the
forceps while embedding (which is our main use for the bunsen flame).
This is the only lab I have ever worked at and I don't know what such a
machine would be called.  If anyone knows the name, or any other
alternative method can you let me know.  Model numbers/companies would
be a bonus too!  Thanks so much for your help.

Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From cgill <@t> marylandgeneral.org  Tue May 18 11:55:26 2010
From: cgill <@t> marylandgeneral.org (Gill, Caula A.)
Date: Tue May 18 11:55:42 2010
Subject: [Histonet] alternative for bunsen burner?
In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3E1E@is-e2k3.grhs.net>
References: 
	<661949901A768E4F9CC16D8AF8F2838C017A3E1E@is-e2k3.grhs.net>
Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9E97@MDGEN-EXCH1.marylandgeneral.org>

Doesn't your embedding station have a forceps warmer on it? You should
not be using an open flame...... 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Tuesday, May 18, 2010 12:12 PM
To: Brandi Higgins; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] alternative for bunsen burner?

Why are you still using a bunsen burner? I believe there is a CAP
question about "No open flames" in the lab. I would suggest finding a
replacement.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi
Higgins
Sent: Tuesday, May 18, 2010 10:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] alternative for bunsen burner?


Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they
don't have to install a gas line.  The pathologist has told me that
there is some machine that can be used instead of a flame to burn the
forceps while embedding (which is our main use for the bunsen flame).
This is the only lab I have ever worked at and I don't know what such a
machine would be called.  If anyone knows the name, or any other
alternative method can you let me know.  Model numbers/companies would
be a bonus too!  Thanks so much for your help.

Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Gayle.Haider <@t> dako.com  Tue May 18 12:06:04 2010
From: Gayle.Haider <@t> dako.com (Gayle Haider)
Date: Tue May 18 12:08:27 2010
Subject: [Histonet] p63
Message-ID: <8B07D141BCDE434285DC12B3290E3FB304A07C0A@exbackca.caus.dako.net>

Dako does still offer a p63 antibody, clone 4A4. It is code number M7247
and it is available in two sizes; 0.2 mL and 1 mL.  Please feel free to
cal Dako Technical Support if you have any questions.

 

Gayle Haider , BSMT(ASCP), CLS

Technical Support Specialist

Dako North America, Inc.

6392 Via Real

Carpinteria, CA  93013 USA

Office: (805) 566-6655 ext. 5323

e-mail: gayle.haider@dako.com  

 

From kdboydhisto <@t> yahoo.com  Tue May 18 12:08:31 2010
From: kdboydhisto <@t> yahoo.com (Kelly Boyd)
Date: Tue May 18 12:08:39 2010
Subject: [Histonet] High Complexity regulations
Message-ID: <26347.70969.qm@web58605.mail.re3.yahoo.com>

I work in?a CLIA certified lab.
?
I do not know how different CAP is, but here is what CLIA requires for High complexity testing personnel:
?

?
TESTING PERSONNEL (42 CFR 493 1489) 
1. Licensed MD, DO or DPM. 
2. Doctorate, master's, or bachelor's in laboratory science. 
3. Associate degree in laboratory science or 
? 60 semester hours including 24 semester hrs of medical lab technology; or, ? 60 semester hrs including 24 hrs of science that includes 6 hrs chemistry, 6 hrs biology, and 12 hrs chemistry, biology or, medical lab tech in any combination and laboratory training that includes either: 
?completion of a clinical lab training program or 
?3 months training in each specialty in which high complexity testing is performed, 
4. Previously qualified or could have qualified as a technologist under federal regulations prior to February 28,1992(42CFR493,1491). 
?
?
?
Hope this helps! BTW, they are very strict about checking your high complexity testing personnel records! 

?
Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
?
?
?
?


      
From foreightl <@t> gmail.com  Tue May 18 12:26:17 2010
From: foreightl <@t> gmail.com (Pat Laurie)
Date: Tue May 18 12:26:30 2010
Subject: [Histonet] alternative for bunsen burner?
In-Reply-To: 
References: 
Message-ID: 

We use one of the "Bactincinerator" sterilizer that Micro uses.  I believe
we got ours through cardinal.

On Tue, May 18, 2010 at 8:30 AM, Brandi Higgins wrote:

> Hello all,
>
> Our hospital is moving our histology department across the hall and have
> asked us if we can find an alternative to the bunsen burner so they don't
> have to install a gas line.  The pathologist has told me that there is some
> machine that can be used instead of a flame to burn the forceps while
> embedding (which is our main use for the bunsen flame).  This is the only
> lab I have ever worked at and I don't know what such a machine would be
> called.  If anyone knows the name, or any other alternative method can you
> let me know.  Model numbers/companies would be a bonus too!  Thanks so much
> for your help.
>
> Brandi Higgins, BS, HT(ASCP)
> _______________________________________________
>  Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie@cellnetix.com
From Bill.Tench <@t> pph.org  Tue May 18 13:02:23 2010
From: Bill.Tench <@t> pph.org (Tench, Bill)
Date: Tue May 18 13:02:36 2010
Subject: [Histonet] urine cytology
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD028630F1@MAIL1.pph.local>

I don't think I know of any labs that do air dried Diff quik type stains
on urine cytologies any more.  You need to use some sort of
concentration technique, and the most frequently used for labs without
liquid-base processing apparatus is the cytospin.  Several companies
make cytospin devices.  High quality preparation with good fixation is
critical, as is specimen quality.  If you don't have training in
cytology (at least cytology preparation) I would stay away from this.

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

Bill.Tench@pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


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From alyssa <@t> alliedsearchpartners.com  Tue May 18 13:03:33 2010
From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson)
Date: Tue May 18 13:03:35 2010
Subject: [Histonet] Correction To Job Post-Lakeland, FL not Plant City,FL
Message-ID: 

Our client, located in Lakeland, FL, has retained Allied Search Partners and
MPath Search Partners to assist in the search for a highly qualified
Histotechnician/Histotechnologist.   This is a unique opportunity to join a
financially strong and growing organization in a key role within the
company.



Shift: Full Time/Permanent, 3am-11am



Requirements: Florida Licensed Technician or Technologist, ASCP preferred



To apply: Please submit resume to alyssa@alliedsearchpartners.com



*No resume will be sent to our client until we speak with you, all resumes
are kept confidential*
From irena.kirbis <@t> hotmail.com  Tue May 18 13:20:06 2010
From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS)
Date: Tue May 18 13:20:32 2010
Subject: [Histonet] Urine Cytology
In-Reply-To: 
References: 
Message-ID: 


there are different slide preparation techiques used in cytology labs - ThinPrep methodology, cytocentrifugation, membrane filtration are the most often used, technique depends on pathologists preferencies and equipment and experiencies of the lab!

hope this helps

Irena
> From: sjkitten@live.com
> To: histonet@lists.utsouthwestern.edu
> Date: Tue, 18 May 2010 10:52:26 -0400
> Subject: [Histonet] Urine Cytology
> 
> 
> Hello,
> 
> My company would like to start doing urine cytologies. Being a histotech i am not 100% sure as to what i need to do this because i have to learned how to di it yet :-) Any suggestions would be greatly appreciated!
> 
> Thanks in Advanced
> 
> S
> 
> 
> 
> _________________________________________________________________
> The New Busy is not the old busy. Search, chat and e-mail from your inbox.
> http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3_______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
_________________________________________________________________
Hotmail: zanesljiva e-po?tna storitev z zmogljivo Microsoftovo za??ito pred ne?eleno po?to
https://signup.live.com/signup.aspx?id=60969
From mward <@t> wfubmc.edu  Tue May 18 13:22:13 2010
From: mward <@t> wfubmc.edu (Martha Ward)
Date: Tue May 18 13:22:21 2010
Subject: [Histonet] p63
In-Reply-To: <8B07D141BCDE434285DC12B3290E3FB304A07C0A@exbackca.caus.dako.net>
References: <8B07D141BCDE434285DC12B3290E3FB304A07C0A@exbackca.caus.dako.net>
Message-ID: <61135F0455D33347B5AAE209B903A30433DEB973@EXCHVS2.medctr.ad.wfubmc.edu>

Thanks but the memo I received dated May 10th stated that Dako's license
to sell M7247, p63 antibody, was due to expire on 31 May 2010.  That was
why I was looking for a new vendor.  Thank you to everyone who responded
to my request.


Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
336-716-2104
 
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle
Haider
Sent: Tuesday, May 18, 2010 1:06 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] p63

Dako does still offer a p63 antibody, clone 4A4. It is code number M7247
and it is available in two sizes; 0.2 mL and 1 mL.  Please feel free to
cal Dako Technical Support if you have any questions.

 

Gayle Haider , BSMT(ASCP), CLS

Technical Support Specialist

Dako North America, Inc.

6392 Via Real

Carpinteria, CA  93013 USA

Office: (805) 566-6655 ext. 5323

e-mail: gayle.haider@dako.com  

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From sgoebel <@t> xbiotech.com  Tue May 18 13:36:56 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Tue May 18 13:37:04 2010
Subject: [Histonet] ICC slide storage
Message-ID: <20100518113656.9e2d9aa830e8449a2412eb1e4f2f067e.0fc00996ad.wbe@email04.secureserver.net>


   So,  again...I'm new to this ICC staining.  I got a epindorf    cells  (and  a PBMC), I made cytospins from them and then fixed them    in  acetone,  then  100& alcohol, then rehydrated them in PBS.  The f   irst day I did the spins the slides looked great, you could definately
   see     become   it pro   fixed  and  po   leave  them  in  4 de   out  in  a slide box.  I   until  ready  to  stain, but I t   leave the fluid sitting in the fridge?
   Thanks guys and gals  =)

   
   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician
   
   XBiotec
   8201 East Riverside Dr. B
   Austin, Texas  
   (512)386-5107
From CIngles <@t> uwhealth.org  Tue May 18 13:40:57 2010
From: CIngles <@t> uwhealth.org (Ingles Claire )
Date: Tue May 18 13:44:59 2010
Subject: [Histonet] Paraffin
Message-ID: 

A question has come up recently about whether or not to completely turn off our embedding center every night or not. I was under the understanding that the constant solidifying and re-melting in the paraffin pot damages the structure of the paraffin and makes it more brittle, etc. Is my memory correct, or do I need to update my "hard drive"?
Claire

From Betsy.Hoffman <@t> comphealth.com  Tue May 18 14:09:03 2010
From: Betsy.Hoffman <@t> comphealth.com (Betsy Hoffman)
Date: Tue May 18 14:09:22 2010
Subject: [Histonet] Histology Jobs Nationwide
Message-ID: <585C6608F313C34D8979DD7A9CD38DFA056C96DE@vslcexmbp02.mychg.com>

Good Afternoon Histotechs.

My name is Betsy and I'm a recruiter with CompHealth, the leader in Allied Health permanent placement.  I could really use your help!  I am currently searching for Certified HT's and HTL's for the following full time positions:


*          HISTOLOGY TECHNOLOGISTS NEEDED IN LAS VEGAS, NV MULTIPLE SHIFTS AVAILABLE!!  (3617857)

*          GREAT HISTOTECHNOLOGIST OPPORTUNITY IN NE PA  (3618429)

*          HISTOLOGY SUPERVISOR NEEDED IN LAS VEGAS  (3618731)

*          NORTH FLORIDA PARADISE IS LOOKING FOR HISTOTECHNOLOGIST WITH IHC  (3618913)

*          HISTOTECH NEEDED TO WORK EVENINGS IN NORTHERN MICHIGAN  (3618927)

*          HISTOLOGIST NEEDED TO WORK DAYS IN EASTERN PA  (3618943)

*          HISTOTECH NEEDED IN COASTAL MASSACHUSETTS!  (3619320)

*          IHC SPECIALIST NEEDED IN COASTAL MASSACHUSETTS!  (3619319)

*          HISTOTECH NEEDED TO WORK DAYS IN SOUTHERN NEW HAMPSHIRE  (3619463)

*          HISTOLOGY TEAM LEADER WITH IHC NEEDED FOR DAY SHIFT IN NORTH CENTRAL MARYLAND  (3619536)

*          HISTOTECHNOLOGIST NEEDED FOR DAY SHIFT IN EASTERN TEXAS .  (3619587)

*          HISTOTECHNOLOGIST NEEDED IN COASTAL CONNECTICUT  (3619624)

*          HISTOECHNOLOGIST NEEDED IN NORTH CENTRAL NY (3619672)


These are just some of the many openings I have in Lab Sciences throughout the country.  If you know of anyone looking for a new position or looking to relocate I can be a great resource and we do pay a generous referral fee.   Please let me know if you can help.

Thanks again for your time.

Cordially,

Betsy Hoffman
Lab Sciences Search Consultant
CompHealth Associates, Inc.
6451 North Federal Highway, Suite 702
Ft. Lauderdale, FL 33308
Toll Free:  1-866-782-9029, X2622
Direct: 1-954-837-2622
Fax: 1-800-420-2329
betsy.hoffman@comphealth.com
www.comphealth.com

ASK ABOUT OUR $250 BONUS FOR REFERRALS!!!

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From mucram11 <@t> comcast.net  Tue May 18 14:38:26 2010
From: mucram11 <@t> comcast.net (Pamela Marcum)
Date: Tue May 18 14:38:30 2010
Subject: [Histonet] High Complexity regulations
In-Reply-To: <26347.70969.qm@web58605.mail.re3.yahoo.com>
Message-ID: <68815825.5138.1274211506737.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>



CAP and CLIA follow the same rules and guidelines so this exactly what the new rules will be June15th.? 



Pam Marcum 

UAMS 





----- Original Message ----- 
From: "Kelly Boyd"  
To: histonet@lists.utsouthwestern.edu 
Sent: Tuesday, May 18, 2010 12:08:31 PM 
Subject: [Histonet] High Complexity regulations 

I work in?a CLIA certified lab. 
? 
I do not know how different CAP is, but here is what CLIA requires for High complexity testing personnel: 
? 

? 
TESTING PERSONNEL (42 CFR 493 1489) 
1. Licensed MD, DO or DPM. 
2. Doctorate, master's, or bachelor's in laboratory science. 
3. Associate degree in laboratory science or 
? 60 semester hours including 24 semester hrs of medical lab technology; or, ? 60 semester hrs including 24 hrs of science that includes 6 hrs chemistry, 6 hrs biology, and 12 hrs chemistry, biology or, medical lab tech in any combination and laboratory training that includes either: 
?completion of a clinical lab training program or 
?3 months training in each specialty in which high complexity testing is performed, 
4. Previously qualified or could have qualified as a technologist under federal regulations prior to February 28,1992(42CFR493,1491). 
? 
? 
? 
Hope this helps! BTW, they are very strict about checking your high complexity testing personnel records! 

? 
Kelly D. Boyd, BS, HTL (ASCP) 
Lab Manager 
? 
? 
? 
? 



_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 






----- Original Message ----- 
From: "Kelly Boyd"  
To: histonet@lists.utsouthwestern.edu 
Sent: Tuesday, May 18, 2010 12:08:31 PM 
Subject: [Histonet] High Complexity regulations 

I work in?a CLIA certified lab. 
? 
I do not know how different CAP is, but here is what CLIA requires for High complexity testing personnel: 
? 

? 
TESTING PERSONNEL (42 CFR 493 1489) 
1. Licensed MD, DO or DPM. 
2. Doctorate, master's, or bachelor's in laboratory science. 
3. Associate degree in laboratory science or 
? 60 semester hours including 24 semester hrs of medical lab technology; or, ? 60 semester hrs including 24 hrs of science that includes 6 hrs chemistry, 6 hrs biology, and 12 hrs chemistry, biology or, medical lab tech in any combination and laboratory training that includes either: 
?completion of a clinical lab training program or 
?3 months training in each specialty in which high complexity testing is performed, 
4. Previously qualified or could have qualified as a technologist under federal regulations prior to February 28,1992(42CFR493,1491). 
? 
? 
? 
Hope this helps! BTW, they are very strict about checking your high complexity testing personnel records! 

? 
Kelly D. Boyd, BS, HTL (ASCP) 
Lab Manager 
? 
? 
? 
? 



_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
From MSHERWOOD <@t> PARTNERS.ORG  Tue May 18 14:57:25 2010
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Tue May 18 14:58:14 2010
Subject: [Histonet] Paraffin
In-Reply-To: 
References: 
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E242F4@PHSXMB30.partners.org>

We leave ours on all the time; we do shut off the cryo center each night (it
frosts up otherwise). 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire 
Sent: Tuesday, May 18, 2010 2:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin

A question has come up recently about whether or not to completely turn off our
embedding center every night or not. I was under the understanding that the
constant solidifying and re-melting in the paraffin pot damages the structure of
the paraffin and makes it more brittle, etc. Is my memory correct, or do I need
to update my "hard drive"?
Claire

_______________________________________________
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From godsgalnow <@t> aol.com  Tue May 18 15:47:08 2010
From: godsgalnow <@t> aol.com (godsgalnow@aol.com)
Date: Tue May 18 15:47:14 2010
Subject: [Histonet] p63 antibody
In-Reply-To: <8C023B4AB999614BA4791BAEB26E2738399E5F@UWHC-MAIL01.uwhis.hosp.wisc.edu>
References: <61135F0455D33347B5AAE209B903A30433DEB970@EXCHVS2.medctr.ad.wfubmc.edu><8C023B4AB999614BA4791BAEB26E2738399E5F@UWHC-MAIL01.uwhis.hosp.wisc.edu>
Message-ID: <388092823-1274215628-cardhu_decombobulator_blackberry.rim.net-1010678923-@bda2106.bisx.prod.on.blackberry>

Biocare has the only IVD p63 I believe
Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: "Sebree Linda A" 
Date: Tue, 18 May 2010 08:47:38 
To: Martha Ward; 
Subject: RE: [Histonet] p63 antibody

We use Biocare's, clone BC4A4. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha
Ward
Sent: Tuesday, May 18, 2010 8:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p63 antibody

This may have been discussed before but....now that Dako is no longer
offering p63, which vendor are most of you switching to?   Thanks in
advance for your help.
 
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab Wake Forest University
Baptist Medical Center
336-716-2104
 
 
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From rsrichmond <@t> gmail.com  Tue May 18 20:17:52 2010
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Tue May 18 20:17:55 2010
Subject: [Histonet] Re: Urine Cytology
Message-ID: 

About urine cytology: It's the job of the cytotechnologist and the
pathologist to decide what urine cytology preparations are wanted. If
the cytotech doesn't know how to do the cytopreparation, there isn't
much a histotechnologist can do to help her.

Bob Richmond
Samurai Pathologist
Knoxville TN

From ratliffjack <@t> hotmail.com  Wed May 19 00:23:57 2010
From: ratliffjack <@t> hotmail.com (Jack Ratliff)
Date: Wed May 19 00:24:11 2010
Subject: [Histonet] Mounting Medium Issues
In-Reply-To: <7267A64D75F58241B577876D8A885631015A9AE2@msgebe41>
References: <7267A64D75F58241B577876D8A885631015A9AE2@msgebe41>
Message-ID: 

Jim,

I must say that I have used Eukitt exclusively for over 13 years and  
the only problem I have had with Eukitt is when I do not use enough  
and especially when coverslipping thicker sections. It is xylenes  
based so maybe you are experiencing excessive evaporation during  
drying??? If you are diluting the Eukitt with xylenes so that it flows  
better (not as viscious) and reduces the chance for air bubbles, this  
might cause excessive shrinkage and/or the problems you are  
experiencing.

Jack

On May 14, 2010, at 3:22 PM, "Herrick, James L. (Jim)"  wrote:

> Hello everyone,
>
> Hope you have all had a good week!!
>
> Has anyone had any negative experiences using Eukitt as your mounting
> media? We embed our specimens (human - iliac crest, animal - femurs,
> tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period
> of time that a fairly large number of our slides are beginning to show
> signs of the medium breaking up below the cover slip. It looks as if
> there are large air pockets left behind. We are not yet sure why  
> this is
> happening. Would any of you geniuses have a resolution for us or  
> know of
> a better mounting media to use with plastic embedded specimens -
> brightfield and fluorescence? Thanks for your expertise. It is very  
> much
> appreciated.
>
> Have a great weekend!!
>
> Jim
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

From ratliffjack <@t> hotmail.com  Wed May 19 00:33:24 2010
From: ratliffjack <@t> hotmail.com (Jack Ratliff)
Date: Wed May 19 00:33:56 2010
Subject: [Histonet] large fibrous bone tissue
In-Reply-To: 
References: <4BED452C020000C50007890E@mail.TSRH.ORG>
	
Message-ID: 

Louise has very good advice here as related to paraffin processing of  
this tissue. I may even add to soak the block a little more before  
taking the final sections. However, have you ever thought of  
processing into MMA resin? If you have these capabilities you may be  
very pleased with the results and find the microtomy less problematic.  
Let me know if or how I can be of assistance!

Jack

On May 15, 2010, at 4:30 AM, louise renton   
wrote:

> I have found this helps.....
> 1. Embed the tissue in  a dep mould, as this provides more  
> stability, then
> 2. Face the block
> 3.. leave in -20 deg freezer overnight
> 4. remove from freezer and cut sections
> 5. If you have multiple blocks to work with, leave them in the  
> freezer until
> ready to cut
>
> regards
> On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia  >wrote:
>
>> How do you process a fibrous bone tissue ( 7 mm thick).  We have use
>> Paraffin Type 9 from Richard allan Scientific to embed works well  
>> with our
>> bone femur( 7 mm) when cutting but on fibrous bone it does not give  
>> us a
>> good result in cutting the blocks. It is like cutting a uterus  
>> tissue but a
>> little bit harder. Please give me your opinion on how to remedy  
>> this kind of
>> tissue not mentioning double embedding method or plastic. Thank you.
>>
>> Reuel Cornelia
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> -- 
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> +27 11 717 2298 (tel & fax)
> 073 5574456 (emergencies only)
> "There are nights when the wolves are silent and only the moon howls".
> George Carlin
> No trees were killed in the sending of this message.
> However, many electrons were terribly inconvenienced.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

From louise.renton <@t> gmail.com  Wed May 19 02:18:58 2010
From: louise.renton <@t> gmail.com (louise renton)
Date: Wed May 19 02:19:44 2010
Subject: [Histonet] large fibrous bone tissue part 2
Message-ID: 

Dear Cornelia & Jack

i disagree about the soaking as this will negate the chilling  effect of the
freezer. The whole idea is to have the block as cold as possible to get
maximum support from the wax*. If the tissue has been processed and
fixed properly, there should not be a necessity for "rehydrating" on water

One thing i forgot to mention wasthat when you embed, try to orientate the
tissue so that the long axis (if there is one) lies in the same direction as
the cutting stroke. when embedding, orientate the tissue at a slight
diagonal, so that the knife dous not continously pass through the tissue on
the cutting stroke - (this works well for skins also, except make sure the
dermis is away from the knife) If this doesn't make sense, let me know and i
will send a graphic privately


*ease of sectioning relies on the embedding material being as close to the
density/stiffness of the tissue being embedded. Thats why you can section
undecal bone in resin....

best regards


On Wed, May 19, 2010 at 7:33 AM, Jack Ratliff wrote:

> Louise has very good advice here as related to paraffin processing of this
> tissue. I may even add to soak the block a little more before taking the
> final sections. However, have you ever thought of processing into MMA resin?
> If you have these capabilities you may be very pleased with the results and
> find the microtomy less problematic. Let me know if or how I can be of
> assistance!
>
> Jack
>
>
> On May 15, 2010, at 4:30 AM, louise renton 
> wrote:
>
> I have found this helps.....
>> 1. Embed the tissue in  a dep mould, as this provides more stability, then
>> 2. Face the block
>> 3.. leave in -20 deg freezer overnight
>> 4. remove from freezer and cut sections
>> 5. If you have multiple blocks to work with, leave them in the freezer
>> until
>> ready to cut
>>
>> regards
>> On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia > >wrote:
>>
>> How do you process a fibrous bone tissue ( 7 mm thick).  We have use
>>> Paraffin Type 9 from Richard allan Scientific to embed works well with
>>> our
>>> bone femur( 7 mm) when cutting but on fibrous bone it does not give us a
>>> good result in cutting the blocks. It is like cutting a uterus tissue but
>>> a
>>> little bit harder. Please give me your opinion on how to remedy this kind
>>> of
>>> tissue not mentioning double embedding method or plastic. Thank you.
>>>
>>> Reuel Cornelia
>>>
>>>
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet@lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>>
>>
>>
>> --
>> Louise Renton
>> Bone Research Unit
>> University of the Witwatersrand
>> Johannesburg
>> South Africa
>> +27 11 717 2298 (tel & fax)
>> 073 5574456 (emergencies only)
>> "There are nights when the wolves are silent and only the moon howls".
>> George Carlin
>> No trees were killed in the sending of this message.
>> However, many electrons were terribly inconvenienced.
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>


-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
From louise.renton <@t> gmail.com  Wed May 19 02:32:24 2010
From: louise.renton <@t> gmail.com (louise renton)
Date: Wed May 19 02:32:40 2010
Subject: [Histonet] Fwd: TDE decalcifier, decalcification commentary
In-Reply-To: 
References: 
	<7442129546479904454@unknownmsgid>
	
Message-ID: 

 Thanks Gayle for your input.

Trouble is, i like the TDE system, and despite not knowing what's in the
solution the IHC results are great. I wasn't sure if endpoint testing could
be used with other acids besides HCl, but you have answered my doubts on
that score. I suspect that, from the smell, it is HCl, but I will have to
dig out old chemistry books to remember how to determine an unknown acid!

anyway, thanks again for your invaluable advice.

best regards






On Tue, May 18, 2010 at 8:52 PM, gayle callis wrote:

>  Hi Teri and Louise,
>
>
>
> There is NO way to tell if a decalcifying solution is used up but you can
> be sure it IS used up.  So the best thing to do is endpoint testing to know
> when the bone is free of calcium.  All decalcifiers become exhausted, so one
> has to replenish the solution with fresh throughout the decal procedure,
> faithfully.  This really doesn't take that much time in the long run, and
> guarantees properly decalcified bone that will not be overexposed to acid -
> a huge no no for decent staining.
>
>
>
> The weight loss/weight gain method is great if you don't have an xray
> machine.   If the solution is an acid e.g. nitric, HCl, or formic, then one
> should do the chemical test or you can play with the weight loss/weight gain
> method.
>
>
>
> Attached are both the chemical and weight loss/weight gain methods, the
> latter was developed for testing nitric acid.  However, this is the easiest
> method to test EDTA other than an xray machine, with the latter being the
> most sensitive i.e. accurate.
>
>
>
> *If the company cannot provide the answer for what is in TDE, then I would
> not use it*. MSDS sometimes tell you, but if not, that company would be
> sayonara from my lab.   I have to know WHAT chemical is doing the work, very
> important to do IHC or routine only staining.   I would rather make it up
> decalcifying solutions myself than buy an product that is unknown, and
> stubbornly proprietary!
>
>
>
> If you do a chemical endpoint test, do NOT stir the decal solution during
> decalcification.  Suspend bones, then collect the aliquot from bottom of the
> container where Ca++ resides.  Remove your samples, and rinse with tap water
> while you do the test.  Return samples to FRESH decalcifying solution and
> proceed with decalcification/testing, etc, etc.
>
>
>
> May not be the answers you wanted but certainly how I have always done bone
> decalcification without problems.
>
>
>
> Take care, Ladies
>
>
>
> Gayle Callis
>
>
>
>
>
>
>
>
>
> *From:* Johnson, Teri [mailto:TJJ@stowers.org]
> *Sent:* Tuesday, May 18, 2010 9:58 AM
> *To:* 'gayle callis'
> *Subject:* Histonet post
>
>
>
> Hey Gayle, thought I'd point this one out to you. Looks like it's right up
> your alley.
>
>
>
> Message: 1
>
> Date: Mon, 17 May 2010 19:30:30 +0200
>
> From: louise renton 
>
> Subject: [Histonet] TDE decalcifier
>
> To: histonet@lists.utsouthwestern.edu
>
> Message-ID:
>
>         
>
> Content-Type: text/plain; charset=ISO-8859-1
>
>
>
> hi all,
>
> although I have been using the TDE decalcification system for awhile, I was
>
> hoping to get some answers from the community. are there any thoughts as to
>
> what the solution is?. And how do you tell if  the solution is "used up" or
>
> not. I wonder if anybody out there has some idea as to whether the solution
>
> is "saturated" with calcium salt or not. as always, I look forward to some
>
> answers.
>
> Best regards
>
> --
>
> Louise Renton
>
> Bone Research Unit
>
> University of the Witwatersrand
>
> Johannesburg
>
> South Africa
>
> +27 11 717 2298 (tel & fax)
>
> 073 5574456 (emergencies only)
>
> "There are nights when the wolves are silent and only the moon howls".
>
> George Carlin
>
> No trees were killed in the sending of this message.
>
> However, many electrons were terribly inconvenienced.
>
>
>
>
>
> I hope things are thawing a bit where you are. I wish it would quit raining
> and warm up here. Supposed to be 80 degrees this weekend. I can't wait!
>
>
>
> All the best,
>
> Teri
>
>
>
>
>
> __________ Information from ESET Smart Security, version of virus signature
> database 5122 (20100517) __________
>
> The message was checked by ESET Smart Security.
>
> http://www.eset.com
>
>
> __________ Information from ESET Smart Security, version of virus signature
> database 5122 (20100517) __________
>
> The message was checked by ESET Smart Security.
>
> http://www.eset.com
>



-- 
 Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.



-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
From WIMERH <@t> si.edu  Wed May 19 05:39:32 2010
From: WIMERH <@t> si.edu (Wimer, Helen)
Date: Wed May 19 05:41:24 2010
Subject: [Histonet] large fibrous bone tissue
In-Reply-To: 
References: <4BED452C020000C50007890E@mail.TSRH.ORG>
	,
	
Message-ID: 

Hi Reul,  It is possible that you are not processing your bone long enough in the tissue processor.  If you would like to discuss this possibility, please email me.  Helen

Helen F Wimer HT (ASCP)
Smithsonian Institution
Department of Vertebrate Zoology
Washington, DC
(301) 496-1391
Wimerh@si.edu
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff [ratliffjack@hotmail.com]
Sent: Wednesday, May 19, 2010 1:33 AM
To: louise renton; Reuel Cornelia
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] large fibrous bone tissue

Louise has very good advice here as related to paraffin processing of
this tissue. I may even add to soak the block a little more before
taking the final sections. However, have you ever thought of
processing into MMA resin? If you have these capabilities you may be
very pleased with the results and find the microtomy less problematic.
Let me know if or how I can be of assistance!

Jack

On May 15, 2010, at 4:30 AM, louise renton 
wrote:

> I have found this helps.....
> 1. Embed the tissue in  a dep mould, as this provides more
> stability, then
> 2. Face the block
> 3.. leave in -20 deg freezer overnight
> 4. remove from freezer and cut sections
> 5. If you have multiple blocks to work with, leave them in the
> freezer until
> ready to cut
>
> regards
> On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia  >wrote:
>
>> How do you process a fibrous bone tissue ( 7 mm thick).  We have use
>> Paraffin Type 9 from Richard allan Scientific to embed works well
>> with our
>> bone femur( 7 mm) when cutting but on fibrous bone it does not give
>> us a
>> good result in cutting the blocks. It is like cutting a uterus
>> tissue but a
>> little bit harder. Please give me your opinion on how to remedy
>> this kind of
>> tissue not mentioning double embedding method or plastic. Thank you.
>>
>> Reuel Cornelia
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> --
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> +27 11 717 2298 (tel & fax)
> 073 5574456 (emergencies only)
> "There are nights when the wolves are silent and only the moon howls".
> George Carlin
> No trees were killed in the sending of this message.
> However, many electrons were terribly inconvenienced.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From kmerriam2003 <@t> yahoo.com  Wed May 19 06:27:42 2010
From: kmerriam2003 <@t> yahoo.com (Kim Merriam)
Date: Wed May 19 06:27:46 2010
Subject: [Histonet] ICC slide storage
In-Reply-To: <20100518113656.9e2d9aa830e8449a2412eb1e4f2f067e.0fc00996ad.wbe@email04.secureserver.net>
References: <20100518113656.9e2d9aa830e8449a2412eb1e4f2f067e.0fc00996ad.wbe@email04.secureserver.net>
Message-ID: <648840.69836.qm@web50303.mail.re2.yahoo.com>

Hi Sarah,

I used to make a?ton of cytospins.? I would air-dry them, fix them in whatever (ethanol or acetone/ethanol), air-dry them again and then wrap the slides in foil, box them up and?store @ -80C.? The slides lasted for months!

When taking them out to use for staining, be sure to allow the wrapped slides to acclimate to RT before you use them.

Happy staining!

Kim

?Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 




________________________________
From: "sgoebel@xbiotech.com" 
To: histonet@lists.utsouthwestern.edu
Sent: Tue, May 18, 2010 2:36:56 PM
Subject: [Histonet] ICC slide storage


? So,? again...I'm new to this ICC staining.? I got a epindorf = tube of
? cells? (and? a PBMC), I made cytospins from them and then fixed them? ? in? acetone,? then? 100& alcohol, then rehydrated them in PBS.? The f? irst day I did the spins the slides looked great, you could definately
? see? = the monocytes.? Then the days that have followed the cells have
? become= lysed, and there is "gradoo" all over the place.? I think that
? it pro= bably has something to do with the fact that the cells weren't
? fixed? and? po=? pped.? My? question is can I fix the slides and then
? leave? them? in? 4 de= grees fridge either in the alcohol or take them
? out? in? a slide box.? I= can see not reconsituting them in the saline
? until? ready? to? stain, but I t= hink I need to fix them and not just
? leave the fluid sitting in the fridge?=? Ahh help!!!

? Thanks guys and gals? =)

? 
? Sarah Goebel, B.A., HT (ASCP)

? Histotechnician

? XBiotec= h USA Inc.

? 8201 East Riverside Dr. B= ldg 4 Suite 100

? Austin, Texas? = 78744

? (512)386-5107
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From brandihiggins <@t> gmail.com  Wed May 19 07:52:29 2010
From: brandihiggins <@t> gmail.com (Brandi Higgins)
Date: Wed May 19 07:52:37 2010
Subject: [Histonet] alt to bunsen burner - thanks
Message-ID: 

Thanks to all who replied to my post about bunsen burner / forcep
sterilization alternatives.  Many of you even included links that were
really helpful.  All of your input was very much appreciated!

Brandi Higgins, BS, HT(ASCP)
From cmiller <@t> physlab.com  Wed May 19 08:34:15 2010
From: cmiller <@t> physlab.com (Cheri Miller)
Date: Wed May 19 08:34:23 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: 
Message-ID: 

That is my understanding as well.

Cheryl A. Miller HT(ASAP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4145 ext. 554
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson
Sent: Tuesday, May 18, 2010 8:03 AM
To: Pamela Marcum
Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Robert Richmond
Subject: Re: [Histonet] Re: Grossing Technician Qualifications

>From my understanding, what I have learned throughout my recruiting career
is that in order to gross you have to have 60 credit hours of COLLEGE
SCIENCE classes, not necessarily a degree, but definitely the 60 credit
hours at least. This requirement is a nationwide requirement from what I
know, and does not vary from state to state.



PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.  Any dissemination, distribution, or copying of this e-mail is strictly prohibited.  If you have received this message in error, please notify the sender immediately and delete this email from your system.

From hadley.bergstrom <@t> usuhs.mil  Wed May 19 08:48:44 2010
From: hadley.bergstrom <@t> usuhs.mil (Hadley Bergstrom)
Date: Wed May 19 08:49:12 2010
Subject: [Histonet] Looking for Gillette super stainless Inoxydable Blades
	(10031694) - Vibratome
Message-ID: 

Hi all,

I am looking for Gillette super stainless Inoxydable Blades (10031694)
for our vibratome.  Apparently Gillette does not make this blade
anymore.  I bought some feather blades from Ted Pella and they tore up
my tissue badly (40 micron sections).

Alternatively would anybody know of a good blade for use with the vibratome?

Thank you very much,

Hadley Bergstrom

From brent.jeter <@t> gwu-hospital.com  Wed May 19 09:36:00 2010
From: brent.jeter <@t> gwu-hospital.com (Jeter, Brent)
Date: Wed May 19 09:36:23 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: 
References: ,
	
Message-ID: 

The regs say at least 60 semesters hours (or equivalent) from an accredited institution are required, and at least 24 of those hours need to be in specific sciences.  See below for specifics. 

Brent Jeter
Anatomic Pathology Supervisor
The George Washington University Hospital
202-715-5076 (phone)
202-715-4691 (fax)
brent.jeter@gwu-hospital.com

ANP.11610 Phase II
If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA regulations?
NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is:
An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 
Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. 
The CLIA regulations on high complexity testing personnel may be found at HC Testing Personnel.
In addition, the CLIA regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at the above Web address and at Grandfathered Exceptions.
It is the responsibility of the laboratory director to determine whether an individual?s education, training and experience satisfies the requirements of this checklist question.
This checklist question applies only to laboratories subject to U.S. regulations.
References
1. Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendments of 1988; final rule. Fed Register. 2003(Oct 1):1070-1071 [42CFR493.1489], 1071-1072 [42CFR493.1491]
2. http://www.naacls.org/news/naacls-news/archives.asp?article_id=599 

________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller [cmiller@physlab.com]
Sent: Wednesday, May 19, 2010 9:34 AM
To: Alyssa Peterson; Pamela Marcum
Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Robert Richmond
Subject: RE: [Histonet] Re: Grossing Technician Qualifications

That is my understanding as well.

Cheryl A. Miller HT(ASAP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4145 ext. 554
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson
Sent: Tuesday, May 18, 2010 8:03 AM
To: Pamela Marcum
Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Robert Richmond
Subject: Re: [Histonet] Re: Grossing Technician Qualifications

>From my understanding, what I have learned throughout my recruiting career
is that in order to gross you have to have 60 credit hours of COLLEGE
SCIENCE classes, not necessarily a degree, but definitely the 60 credit
hours at least. This requirement is a nationwide requirement from what I
know, and does not vary from state to state.



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From mjones <@t> WNJ.ORG  Wed May 19 09:37:17 2010
From: mjones <@t> WNJ.ORG (Mary Jones)
Date: Wed May 19 09:37:32 2010
Subject: [Histonet] RE: Histonet Digest, Vol 78, Issue 25
In-Reply-To: <20100518174259.EBD7546B7E@eprism.internal.wnj.bz>
Message-ID: <864481E5A1EFC947B19A0A4AA35E0952041F0138@EXCHANGESVR.Internal.wnj.bz>

Can you charge for two different stains for the urine cytospin?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Tuesday, May 18, 2010 12:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 78, Issue 25

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Today's Topics:

   1. Urine Cytology (S R)
   2. alternative for bunsen burner? (Brandi Higgins)
   3. Grossing assistants-pathologist assistants (Tench, Bill)
   4. RE: Urine Cytology (sgoebel@xbiotech.com)
   5. RE: alternative for bunsen burner? (Mike Pence)
   6. Re: alternative for bunsen burner? (Jennifer MacDonald)
   7. Re: alternative for bunsen burner? (BSullivan@shorememorial.org)
   8. RE: alternative for bunsen burner? (Jackie M O'Connor)
   9. RE: alternative for bunsen burner? (Gill, Caula A.)


----------------------------------------------------------------------

Message: 1
Date: Tue, 18 May 2010 10:52:26 -0400
From: S R 
Subject: [Histonet] Urine Cytology
To: histo net 
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"


Hello,

My company would like to start doing urine cytologies.  Being a
histotech i am not 100% sure as to what i need to do this because i have
to learned how to di it yet :-)  Any suggestions would be greatly
appreciated!

Thanks in Advanced

S

 
 		 	   		  
_________________________________________________________________
The New Busy is not the old busy. Search, chat and e-mail from your
inbox.
http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:
ON:WL:en-US:WM_HMP:042010_3

------------------------------

Message: 2
Date: Tue, 18 May 2010 11:30:11 -0400
From: Brandi Higgins 
Subject: [Histonet] alternative for bunsen burner?
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they
don't
have to install a gas line.  The pathologist has told me that there is
some
machine that can be used instead of a flame to burn the forceps while
embedding (which is our main use for the bunsen flame).  This is the
only
lab I have ever worked at and I don't know what such a machine would be
called.  If anyone knows the name, or any other alternative method can
you
let me know.  Model numbers/companies would be a bonus too!  Thanks so
much
for your help.

Brandi Higgins, BS, HT(ASCP)


------------------------------

Message: 3
Date: Tue, 18 May 2010 08:54:45 -0700
From: "Tench, Bill" 
Subject: [Histonet] Grossing assistants-pathologist assistants
To: histonet@lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD028630EB@MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii

Yes, the Pathology Assistant national organization is a great resource
as is the CAP.  As I said before, please remember that the CAP
inspection standards represent compliance with FEDERAL CMS standards.  I
would encourage contact with the CAP LAP  in regard to these FEDERAL
standards.  Individual states may also have requirements specific for
that state, which certainly may complicate things, so contact with your
state pathology society will help with that. 

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

Bill.Tench@pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


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------------------------------

Message: 4
Date: Tue, 18 May 2010 09:03:23 -0700
From: sgoebel@xbiotech.com
Subject: RE: [Histonet] Urine Cytology
To: "S R" 
Cc: histo net 
Message-ID:
	
<20100518090323.9e2d9aa830e8449a2412eb1e4f2f067e.9ba84e7acf.wbe@email04.
secureserver.net>
	
Content-Type: text/plain; charset="utf-8"


   The  way  we always did it was to get the specimen fresh (so 24 hou
urine  is usually not a good specimen), then make two cytospins.  Sta
in  one  with  diff quik and one with a PAP stain.  We never made cell
   bl   air d
   Sarah Goebel, B.A., HT (ASCP)
   XBiotech USA Inc.

   
   8201 East
   <
   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] Urine Cytology
   From: S R 
   Date: Tue, May 18, 2010 7:52 am
   To: histo net 
   Hello,
   My  company  would  like  to  start  doing  urine  cytologies. Being
a
   histotech  i   have  to  learned  ho   greatly appreciated!
   Thanks in Advanced
   S
   _________________________________________________________________
   The  New  Busy  is not the old busy. Search, chat and e-mail from
your
   inbox.<
?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3_______________
   _____   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
References

   1. 3D"http://www.windowslive.com/campaign/thenewbusy   2.
3D"http://lists.utsouthwestern.edu/mailman/listin

------------------------------

Message: 5
Date: Tue, 18 May 2010 11:11:52 -0500
From: "Mike Pence" 
Subject: RE: [Histonet] alternative for bunsen burner?
To: "Brandi Higgins" ,
	
Message-ID:
	<661949901A768E4F9CC16D8AF8F2838C017A3E1E@is-e2k3.grhs.net>
Content-Type: text/plain;	charset="us-ascii"

Why are you still using a bunsen burner? I believe there is a CAP
question about "No open flames" in the lab. I would suggest finding a
replacement.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi
Higgins
Sent: Tuesday, May 18, 2010 10:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] alternative for bunsen burner?


Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they
don't have to install a gas line.  The pathologist has told me that
there is some machine that can be used instead of a flame to burn the
forceps while embedding (which is our main use for the bunsen flame).
This is the only lab I have ever worked at and I don't know what such a
machine would be called.  If anyone knows the name, or any other
alternative method can you let me know.  Model numbers/companies would
be a bonus too!  Thanks so much for your help.

Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 6
Date: Tue, 18 May 2010 09:27:53 -0700
From: Jennifer MacDonald 
Subject: Re: [Histonet] alternative for bunsen burner?
To: Brandi Higgins 
Cc: histonet@lists.utsouthwestern.edu,
	histonet-bounces@lists.utsouthwestern.edu
Message-ID:
	

Content-Type: text/plain; charset="US-ASCII"

a bacti incinerator




Brandi Higgins  
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/18/2010 09:00 AM

To
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] alternative for bunsen burner?






Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they
don't
have to install a gas line.  The pathologist has told me that there is 
some
machine that can be used instead of a flame to burn the forceps while
embedding (which is our main use for the bunsen flame).  This is the
only
lab I have ever worked at and I don't know what such a machine would be
called.  If anyone knows the name, or any other alternative method can
you
let me know.  Model numbers/companies would be a bonus too!  Thanks so 
much
for your help.

Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 7
Date: Tue, 18 May 2010 12:46:40 -0400
From: BSullivan@shorememorial.org
Subject: Re: [Histonet] alternative for bunsen burner?
To: Brandi Higgins 
Cc: histonet@lists.utsouthwestern.edu,
	histonet-bounces@lists.utsouthwestern.edu
Message-ID:
	

	
Content-Type: text/plain; charset=US-ASCII

We use an alcohol burner that has a wick inside. It works well for us.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


 

             Brandi Higgins

             
To 
             Sent by:                  histonet@lists.utsouthwestern.edu

             histonet-bounces@
cc 
             lists.utsouthwest

             ern.edu
Subject 
                                       [Histonet] alternative for bunsen

                                       burner?

             05/18/2010 11:30

             AM

 

 

 

 





Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they
don't
have to install a gas line.  The pathologist has told me that there is
some
machine that can be used instead of a flame to burn the forceps while
embedding (which is our main use for the bunsen flame).  This is the
only
lab I have ever worked at and I don't know what such a machine would be
called.  If anyone knows the name, or any other alternative method can
you
let me know.  Model numbers/companies would be a bonus too!  Thanks so
much
for your help.

Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 8
Date: Tue, 18 May 2010 11:52:37 -0500
From: Jackie M O'Connor 
Subject: RE: [Histonet] alternative for bunsen burner?
To: "Mike Pence" 
Cc: histonet@lists.utsouthwestern.edu,
	histonet-bounces@lists.utsouthwestern.edu
Message-ID:
	

Content-Type: text/plain; charset="US-ASCII"

I think that's the crux of her question - what can she use as an 
alternative?   You can find forceps warmers here.   
http://americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=EQ
FW-120
Although, I still miss the ol' bunsen burner.   It was great for burning

away little fragments of tissue from between the teeth - of the forceps,

not my teeth. 



From:
"Mike Pence" 
To:
"Brandi Higgins" , 

Date:
05/18/2010 11:42 AM
Subject:
RE: [Histonet] alternative for bunsen burner?
Sent by:
histonet-bounces@lists.utsouthwestern.edu



Why are you still using a bunsen burner? I believe there is a CAP
question about "No open flames" in the lab. I would suggest finding a
replacement.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi
Higgins
Sent: Tuesday, May 18, 2010 10:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] alternative for bunsen burner?


Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they
don't have to install a gas line.  The pathologist has told me that
there is some machine that can be used instead of a flame to burn the
forceps while embedding (which is our main use for the bunsen flame).
This is the only lab I have ever worked at and I don't know what such a
machine would be called.  If anyone knows the name, or any other
alternative method can you let me know.  Model numbers/companies would
be a bonus too!  Thanks so much for your help.

Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 9
Date: Tue, 18 May 2010 12:55:26 -0400
From: "Gill, Caula A." 
Subject: RE: [Histonet] alternative for bunsen burner?
To: "Mike Pence" , "Brandi Higgins"
	,

Message-ID:
	
<087A9911BBAFDE4B8151CB148586E2C23A9E97@MDGEN-EXCH1.marylandgeneral.org>
	
Content-Type: text/plain;	charset="us-ascii"

Doesn't your embedding station have a forceps warmer on it? You should
not be using an open flame...... 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Tuesday, May 18, 2010 12:12 PM
To: Brandi Higgins; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] alternative for bunsen burner?

Why are you still using a bunsen burner? I believe there is a CAP
question about "No open flames" in the lab. I would suggest finding a
replacement.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi
Higgins
Sent: Tuesday, May 18, 2010 10:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] alternative for bunsen burner?


Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they
don't have to install a gas line.  The pathologist has told me that
there is some machine that can be used instead of a flame to burn the
forceps while embedding (which is our main use for the bunsen flame).
This is the only lab I have ever worked at and I don't know what such a
machine would be called.  If anyone knows the name, or any other
alternative method can you let me know.  Model numbers/companies would
be a bonus too!  Thanks so much for your help.

Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 78, Issue 25
****************************************

From TJJ <@t> stowers.org  Wed May 19 09:48:59 2010
From: TJJ <@t> stowers.org (Johnson, Teri)
Date: Wed May 19 09:49:10 2010
Subject: [Histonet] Re: looking for Gillette super stainless Inoxydable
 Blades - Vibratome
Message-ID: 

Hi Hadley,

We get our blades from EMS (Electron Microscopy Sciences): http://www.emsdiasum.com/microscopy/products/preparation/blades.aspx?mm=10#71990

On this page you'll find the injector blades. Further down they have the double edge blade you can break in half and use in the holder. Try those and see if they work for you.

Best wishes,

Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO


From laurie.colbert <@t> huntingtonhospital.com  Wed May 19 09:59:30 2010
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Wed May 19 09:59:43 2010
Subject: [Histonet] IHC Validation on new instrument
Message-ID: <57BE698966D5C54EAE8612E8941D768308BCAF81@EXCHANGE3.huntingtonhospital.com>

What do others do when validating a new model of a piece of equipment -
same manufacturer, same basic staining process, but an updated version
of the equipment?

 

I've been told the protocols should be the same and that we only need to
run three controls with three different but similar protocols to
determine what looks best.  Do you all think that is thorough enough, or
would you run actual patient cases and compare old and new equipment?  I
don't see where the CAP checklist refers to new equipment - just new
antibodies and new antibody lots.

 

Laurie Colbert

From Wanda.Smith <@t> HCAhealthcare.com  Wed May 19 10:20:20 2010
From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com)
Date: Wed May 19 10:20:31 2010
Subject: [Histonet] Yellow Walls in Pathology-Deja Vu
Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA138C209A72@NADCWPMSGCMS03.hca.corpad.net>

Good Morning to All,
A few years ago I sent a inquiry to Histonet asking if anyone has ever had this issue in their tissue processing area?  We are having this issue again and I cannot pinpoint any changes or issues that have arisen.
We have our VIP tissue processor, paraffin pot and embedding center next to each other.  Our Immuno stainer is across the room and I have 4 microtomy cutting stations on the other side of the room.  Good ventilation all around.  The ceiling grids are yellow, the walls and on top of the IHC stainer lid.
Anybody else ever had this problem?????
Thanks,
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax


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From Erin.Martin <@t> ucsf.edu  Wed May 19 11:09:22 2010
From: Erin.Martin <@t> ucsf.edu (Martin, Erin)
Date: Wed May 19 11:09:55 2010
Subject: [Histonet] Freezing spray artifact
Message-ID: <379A927A452F3D43A3C8705F4E67905F0FC1ECB56C@EX05.net.ucsf.edu>

Has anyone run into a problem or artifact from freezing spray?  I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

From kalschev <@t> svm.vetmed.wisc.edu  Wed May 19 11:11:46 2010
From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur)
Date: Wed May 19 11:11:59 2010
Subject: [Histonet] fibrous bone tissue
Message-ID: <27F11DE4E89F4027A5BAF7641D90DB85@vetmed.wisc.edu>

The thickness of tissue would work better if thinner, however, if you can infiltrate in paraffin overnight for an extra 12-18 hours it should embed and section just fine.  I face the block warm; soak on ice for a short time and then section. I find that taking the cuts in deeper, after the block warms up in the chuck ( brush with a damp gauze a few times) , gives me beautiful sections of fibrous area and bone. Perhaps section thicker.  The blade must be very sharp. A D profile steel blade can be used ( although I have had good results with dispo blades).  I have also adjusted my knife angle more straight up.  Vicki
From marktarango <@t> gmail.com  Wed May 19 11:15:23 2010
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Wed May 19 11:15:39 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: 
References: 
	
	
Message-ID: 

You would think the ASCP would have some kind of grossing tech qualification
or certification for those who aren't PA's but have the 60 semester hours
and training.  Just thought I'd throw that out there.

Mark Tarango

On Wed, May 19, 2010 at 7:36 AM, Jeter, Brent
wrote:

> The regs say at least 60 semesters hours (or equivalent) from an accredited
> institution are required, and at least 24 of those hours need to be in
> specific sciences.  See below for specifics.
>
> Brent Jeter
> Anatomic Pathology Supervisor
> The George Washington University Hospital
> 202-715-5076 (phone)
> 202-715-4691 (fax)
> brent.jeter@gwu-hospital.com
>
> ANP.11610 Phase II
> If individuals other than a pathologist or pathology resident assist in
> gross examinations, do such individuals qualify as high complexity testing
> personnel under CLIA regulations?
> NOTE: The laboratory director may delegate the dissection of specimens to
> non-pathologist individuals; these individuals must be qualified as high
> complexity testing personnel under CLIA regulations. The minimum
> training/experience required of such personnel is:
> An earned associate degree in a laboratory science or medical laboratory
> technology, obtained from an accredited institution, OR
> Education/training equivalent to the above that includes at least 60
> semester hours or equivalent from an accredited institution. This education
> must include 24 semester hours of medical laboratory technology courses, OR
> 24 semester hours of science courses that includes 6 semester hours of
> chemistry, 6 semester hours of biology, and 12 semester hours of chemistry,
> biology or medical laboratory technology in any combination. In addition,
> the individual must have laboratory training including either completion of
> a clinical laboratory training program approved or accredited by the ABHES,
> NAACLA, or other organization approved by HHS (note that this training may
> be included in the 60 semester hours listed above), OR at least 3 months
> documented laboratory training in each specialty in which the individual
> performs high complexity testing.
> The CLIA regulations on high complexity testing personnel may be found at
> HC Testing Personnel.
> In addition, the CLIA regulations include exceptions for grandfathered
> individuals; these regulations (42CFR493.1489 and 1491) may be found at the
> above Web address and at Grandfathered Exceptions.
> It is the responsibility of the laboratory director to determine whether an
> individual?s education, training and experience satisfies the requirements
> of this checklist question.
> This checklist question applies only to laboratories subject to U.S.
> regulations.
> References
> 1. Department of Health and Human Services, Centers for Medicare and
> Medicaid Services. Clinical laboratory improvement amendments of 1988; final
> rule. Fed Register. 2003(Oct 1):1070-1071 [42CFR493.1489], 1071-1072
> [42CFR493.1491]
> 2. http://www.naacls.org/news/naacls-news/archives.asp?article_id=599
>
> ________________________________________
> From: histonet-bounces@lists.utsouthwestern.edu [
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller [
> cmiller@physlab.com]
> Sent: Wednesday, May 19, 2010 9:34 AM
> To: Alyssa Peterson; Pamela Marcum
> Cc: histonet@lists.utsouthwestern.edu;
> histonet-bounces@lists.utsouthwestern.edu; Robert Richmond
> Subject: RE: [Histonet] Re: Grossing Technician Qualifications
>
> That is my understanding as well.
>
> Cheryl A. Miller HT(ASAP)cm
> Histology/Cytology Prep Supervisor
> Physicians Laboratory Services
> Omaha, NE. 402 731 4145 ext. 554
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson
> Sent: Tuesday, May 18, 2010 8:03 AM
> To: Pamela Marcum
> Cc: histonet@lists.utsouthwestern.edu;
> histonet-bounces@lists.utsouthwestern.edu; Robert Richmond
> Subject: Re: [Histonet] Re: Grossing Technician Qualifications
>
> >From my understanding, what I have learned throughout my recruiting career
> is that in order to gross you have to have 60 credit hours of COLLEGE
> SCIENCE classes, not necessarily a degree, but definitely the 60 credit
> hours at least. This requirement is a nationwide requirement from what I
> know, and does not vary from state to state.
>
>
>
> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If
> you are not the addressee intended / indicated or agent responsible for
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> possession of confidential and privileged information.  Any dissemination,
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> received this message in error, please notify the sender immediately and
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> _______________________________________________
> Histonet mailing list
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> UHS Confidentiality Notice:  This e-mail message, including any
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>
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From Rcartun <@t> harthosp.org  Wed May 19 11:40:08 2010
From: Rcartun <@t> harthosp.org (Richard Cartun)
Date: Wed May 19 11:40:18 2010
Subject: [Histonet] Re: [IHCRG] candida
In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB22669E7C3@chi2k3ms01.columbuschildrens.net>
References: <979FF5962E234F45B06CF0DB7C1AABB22669E7C3@chi2k3ms01.columbuschildrens.net>
Message-ID: <4BF3DC27.7400.0077.1@harthosp.org>

Check with ViroStat (207-856-6620) in Portland, ME.  They have some excellent infectious disease antibodies.

Richard 

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Houston, Ronald"  5/19/2010 12:31 PM >>>
Anyone aware of an antibody to Candida albicans that works in paraffin
sections?

Thanks

 

Ronnie Houston, MS HT(ASCP)QIHC

Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com 

 

700 Children's Drive

Columbus, OH 43205

(P) 614-722-5450

(F) 614-722-2899

ronald.houston@nationwidechildrens.org 

www.NationwideChildrens.org  

 

"One person with passion is better than forty people merely interested."
~ E.M. Forster

 



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From POWELL_SA <@t> mercer.edu  Wed May 19 12:15:37 2010
From: POWELL_SA <@t> mercer.edu (Shirley A. Powell)
Date: Wed May 19 12:15:44 2010
Subject: [Histonet] RE: Freezing spray artifact
In-Reply-To: <379A927A452F3D43A3C8705F4E67905F0FC1ECB56C@EX05.net.ucsf.edu>
References: <379A927A452F3D43A3C8705F4E67905F0FC1ECB56C@EX05.net.ucsf.edu>
Message-ID: <9BF995BC0E47744E9673A41486E24EE2268C5518AA@MERCERMAIL.MercerU.local>

There is a text written by the late Lee Luna and Samuel Wesley Thompson entitled An Atlas of Artifacts in this the artifact from freeze spray is pictured.  The ISBN # is 0-398-03624-1 Published by Charles C. Thomas, Publisher.  

Shirley Powell

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Wednesday, May 19, 2010 12:09 PM
To: histonet
Subject: [Histonet] Freezing spray artifact

Has anyone run into a problem or artifact from freezing spray?  I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From liz <@t> premierlab.com  Wed May 19 12:21:32 2010
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Wed May 19 12:21:39 2010
Subject: [Histonet] IHC Validation on new instrument
In-Reply-To: <57BE698966D5C54EAE8612E8941D768308BCAF81@EXCHANGE3.huntingtonhospital.com>
Message-ID: 

We perform our entire validation process as a new piece of equipment.
Our validation protocols are quite extensive, up to about 85 pages long
on each piece of major equipment, at least that's what it was for our
new prisma stainer and glass coverslipper.  We perform an
installation/operational qualification protocol or an IOQ.  If we move
the instrument we also do the same thing, we already have the protocol
written which takes most of the time we just execute it again.  We are a
GLP lab so we work off a Validation Master Plan that basically tells us
how we are going to validate each piece of equipment in the lab.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Wednesday, May 19, 2010 9:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation on new instrument

What do others do when validating a new model of a piece of equipment -
same manufacturer, same basic staining process, but an updated version
of the equipment?

 

I've been told the protocols should be the same and that we only need to
run three controls with three different but similar protocols to
determine what looks best.  Do you all think that is thorough enough, or
would you run actual patient cases and compare old and new equipment?  I
don't see where the CAP checklist refers to new equipment - just new
antibodies and new antibody lots.

 

Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From renafail <@t> bellsouth.net  Wed May 19 12:30:49 2010
From: renafail <@t> bellsouth.net (Rena Fail)
Date: Wed May 19 12:31:02 2010
Subject: [Histonet] Freezing spray artifact
In-Reply-To: <379A927A452F3D43A3C8705F4E67905F0FC1ECB56C@EX05.net.ucsf.edu>
References: <379A927A452F3D43A3C8705F4E67905F0FC1ECB56C@EX05.net.ucsf.edu>
Message-ID: <96641.83001.qm@web180301.mail.gq1.yahoo.com>

Erin,
Holding the can too close and/or spraying for a prolonged period both cause freeze artifact. Look at your block, it will not have a smooth look to the paraffin.?it will?have lines it much like a cracked piece of glass. the tissue will appear cracked on the waterbath and will even separate along these lines.?The cracks can be seen microscopically amd interfere with the architecture?of the tissue. 
Rena Fail



?----- Original Message ----
From: "Martin, Erin" 
To: histonet 
Sent: Wed, May 19, 2010 12:09:22 PM
Subject: [Histonet] Freezing spray artifact

Has anyone run into a problem or artifact from freezing spray?? I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From LSebree <@t> uwhealth.org  Wed May 19 12:38:32 2010
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Wed May 19 12:38:42 2010
Subject: [Histonet] Freezing spray artifact
In-Reply-To: <379A927A452F3D43A3C8705F4E67905F0FC1ECB56C@EX05.net.ucsf.edu>
References: <379A927A452F3D43A3C8705F4E67905F0FC1ECB56C@EX05.net.ucsf.edu>
Message-ID: <8C023B4AB999614BA4791BAEB26E2738399E63@UWHC-MAIL01.uwhis.hosp.wisc.edu>

Erin,  
One can certainly get cracking of the tissue and surrounding paraffin
from a too intense application of freezing spray.  This is usually
grossly visible, at least in the paraffin. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin,
Erin
Sent: Wednesday, May 19, 2010 11:09 AM
To: histonet
Subject: [Histonet] Freezing spray artifact

Has anyone run into a problem or artifact from freezing spray?  I think
we may be having a problem with it but I can't find any pictures or
descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From mbrooks <@t> incytepathology.com  Wed May 19 12:44:40 2010
From: mbrooks <@t> incytepathology.com (Matt Brooks)
Date: Wed May 19 12:44:53 2010
Subject: [Histonet] IHC Validation on microwaved tissue
Message-ID: <706224670091FE47997AEF88EFADE7CA01550C9A@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>

Hello All,

We have new microwave tissue processor, until this purchase all tissue
has been placed on a standard VIP processor.  My medical director would
like for most of our 125 antibodies worked up on microwave processed
tissue.  I have been challenged with getting specimens to process on the
instrument for IHC antibody validation purposes.  Initially we will be
processing GI biopsy specimens, but we will eventually progress to other
biopsy tissues and so on.  I have had success getting some colon cancer
cases, some tonsil and other tissue. We will be mainly performing H.
pylori, which is hard to get a large enough specimen to obtain a sample
for this purpose.  Anyway I was wondering how other labs are handling
validation in this situation.  Thank you for your time in this matter. 

Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
mbrooks@incytepathology.com
509-892-2744


From Bill.Tench <@t> pph.org  Wed May 19 12:52:21 2010
From: Bill.Tench <@t> pph.org (Tench, Bill)
Date: Wed May 19 12:52:40 2010
Subject: [Histonet] charging for cytospins
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863103@MAIL1.pph.local>

Can you charge for two different stains for the urine cytospin?

 

The answer to this question is a "depends."  If you are just doing any
of the "optional" stains that may be used on a cytology preparation
(namely Pap, H&E, romanovsky) you are NOT permitted to charge for each
of these (they are not considered "special stains").  If you did an iron
stain, or melanin stain, or mucin stain, you could charge separately for
these "special" stains.

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

Bill.Tench@pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


mail2.pph.org made the following annotations
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From dlschneider <@t> gmail.com  Wed May 19 12:57:55 2010
From: dlschneider <@t> gmail.com (Daniel Schneider)
Date: Wed May 19 12:58:07 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: 
References: 
	
	
	
Message-ID: 

Just to clarify or perhaps cloud the picture a little more

Not all grossing is "grossing."

So when we're talking about transferring small biopsies, in their
entirety, from a formalin container to a cassette, and describing the size,
number, and color of the tissue pieces submitted, with no dissection or
knowledge of anatomy required, that's not "grossing" in the strict sense, at
least according to CAP the last time I looked, which admittedly was a couple
of years ago.

So what sort of educational achievements, training, or credentials are
required for the above?

Dan Schneider
From mfisher <@t> ecrmc.org  Wed May 19 12:57:39 2010
From: mfisher <@t> ecrmc.org (Marcia Fisher)
Date: Wed May 19 13:05:05 2010
Subject: [Histonet] Temp Histo Tech Needed
References: <20100519173446.EDE4E1BC043C_BF42136F@sophos.ecrmc.ci.el-centro.ca.us>
Message-ID: <3ACBB5D73A417547A01970CD3EB55093047B626B@MAIL1.ecrmc.ci.el-centro.ca.us>

We are in need of a temporary, ASCP certified histotech for 4-6 weeks beginning June 21 at El Centro Regional Medical Center.  Located just 2 hours east of San Diego, 100 miles south of Palm Springs and only 60 miles west of Yuma.  Our new lab was just completed in February, 2010 and is state-of-the-art. 

El Centro Regional Medical Center is a nonprofit, community based hospital owned by the City of El Centro. For over 50 years, ECRMC has provided medical care to the Imperial Valley and has become a leading healthcare system as an excellent source for health care services.

In addition to the medical center, the community is served by two Outpatient Centers, a Wound Healing Center, a Children's Specialist Clinic and various educational programs including Asthma and Diabetes.

With more than 150 accredited physicians and over 900 employees, El Centro Regional Medical Center strives for excellence in the Imperial Valley.

Please contact me thru work email if interested and more details.   mfisher@ecrmc.org

M. Fisher

El Centro Regional Medical Center

1415 Ross Ave
El Centro, CA  92243
760-339-7267
760-482-5365(F)
www.ecrmc.org  
 
Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments.

________________________________

   
From kenneth.a.troutman <@t> Vanderbilt.Edu  Wed May 19 13:06:27 2010
From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A)
Date: Wed May 19 13:06:41 2010
Subject: [Histonet] IHC Validation on new instrument
Message-ID: <7B310892042DA74CB3590053F424CFE60B83F97011@ITS-HCWNEM06.ds.Vanderbilt.edu>


Hi Laurie,

I have a Benchmark Ultra and a Benchmark XT from Ventana and they follow the basic steps, similar protocols and I needed to revalidate everything.  They are sufficiently different (in my experience) that it warranted a complete revalidation.  A part of the reasoning for this was some of the bulk reagents were different (a consideration that may lead you to not completely revalidate).  For me, there were a few protocols that were quite different from one to the other.  I would go to the trouble up front, that way there is no question later.

Good luck!

Ashley Troutman BS, HT(ASCP) QIHC
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4532
Nashville, TN  37232
615-343-9134

Message: 4
Date: Wed, 19 May 2010 07:59:30 -0700
From: "Laurie Colbert" 
Subject: [Histonet] IHC Validation on new instrument
To: 
Message-ID:
        <57BE698966D5C54EAE8612E8941D768308BCAF81@EXCHANGE3.huntingtonhospital.com>

Content-Type: text/plain;       charset="us-ascii"

What do others do when validating a new model of a piece of equipment -
same manufacturer, same basic staining process, but an updated version
of the equipment?



I've been told the protocols should be the same and that we only need to
run three controls with three different but similar protocols to
determine what looks best.  Do you all think that is thorough enough, or
would you run actual patient cases and compare old and new equipment?  I
don't see where the CAP checklist refers to new equipment - just new
antibodies and new antibody lots.



Laurie Colbert
From cindy.deriso <@t> yale.edu  Wed May 19 13:20:42 2010
From: cindy.deriso <@t> yale.edu (Cindy DeRiso)
Date: Wed May 19 13:20:55 2010
Subject: [Histonet] PPE
Message-ID: <4BF42BFA.6030006@yale.edu>

Does any one use or require gloves when cutting and embedding? How about 
safety goggles when embedding?
Thanks in advance-
Cindy DeRiso
Yale University Pathology

From sgoebel <@t> xbiotech.com  Wed May 19 13:37:30 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Wed May 19 13:37:51 2010
Subject: [Histonet] Freezing spray artifact
Message-ID: <20100519113730.9e2d9aa830e8449a2412eb1e4f2f067e.24d0cf7c87.wbe@email04.secureserver.net>


   [DEL: 3D"" :DEL] 
   Picture  on the  left has the artifact, picture on the right is good
   to go.  Hope this
   Sarah Goebel, B.A., HT (ASCP)
   Histotechnician
   <   XBiotech USA Inc.
   8201 Eas      (512)386-51
   -------- Original Message --------
   Subject: [Histonet] Freezing spray artifact
   From: "Martin, Erin" 
   Date: Wed, May 19, 2010 9:09 am
   To: histonet 
   Has anyone run into a problem or artifact from freezing spray? I think
   we     descriptio   Thanks in advance,
   Erin
   Erin Martin, Histology Supervisor
   UCSF Department of Dermatopathology
   415-353-7248
   _______________________________________________
   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet

References

   1. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
From Janice.Mahoney <@t> alegent.org  Wed May 19 13:38:43 2010
From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A)
Date: Wed May 19 13:38:53 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: 
References: 
	
	
	
	
Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A1B6@EXCHMBC2.ad.ah.local>

Things have changed.  All "grossing" is back to being grossing.
Jan Mahoney
Omaha, NE

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider
Sent: Wednesday, May 19, 2010 12:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Grossing Technician Qualifications

Just to clarify or perhaps cloud the picture a little more

Not all grossing is "grossing."

So when we're talking about transferring small biopsies, in their
entirety, from a formalin container to a cassette, and describing the size,
number, and color of the tissue pieces submitted, with no dissection or
knowledge of anatomy required, that's not "grossing" in the strict sense, at
least according to CAP the last time I looked, which admittedly was a couple
of years ago.

So what sort of educational achievements, training, or credentials are
required for the above?

Dan Schneider
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person.

The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees.  Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.  If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.  Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening.  Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.  Thank you for your cooperation.


From liz <@t> premierlab.com  Wed May 19 14:15:08 2010
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Wed May 19 14:15:13 2010
Subject: [Histonet] PPE
In-Reply-To: <4BF42BFA.6030006@yale.edu>
Message-ID: 

I require gloves when sectioning only because it helps eliminate
epithelial floaters, with respects to embedding the tech can choose to
or not to use gloves.  No safety goggles are required.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy
DeRiso
Sent: Wednesday, May 19, 2010 12:21 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] PPE

Does any one use or require gloves when cutting and embedding? How about

safety goggles when embedding?
Thanks in advance-
Cindy DeRiso
Yale University Pathology

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From MSHERWOOD <@t> PARTNERS.ORG  Wed May 19 14:16:44 2010
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Wed May 19 14:17:34 2010
Subject: [Histonet] PPE
In-Reply-To: <4BF42BFA.6030006@yale.edu>
References: <4BF42BFA.6030006@yale.edu>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E242FF@PHSXMB30.partners.org>

We wear gloves when handling tissue and embedding.  No gloves for sectioning and
no safety goggles.   

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DeRiso
Sent: Wednesday, May 19, 2010 2:21 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] PPE

Does any one use or require gloves when cutting and embedding? How about 
safety goggles when embedding?
Thanks in advance-
Cindy DeRiso
Yale University Pathology

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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From dlschneider <@t> gmail.com  Wed May 19 14:18:46 2010
From: dlschneider <@t> gmail.com (Daniel Schneider)
Date: Wed May 19 14:19:00 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A1B6@EXCHMBC2.ad.ah.local>
References: 
	
	
	
	
	<8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A1B6@EXCHMBC2.ad.ah.local>
Message-ID: 

Really. Are you sure?

"Received in formalin, labeled with the patient's name and number, the
specimen consists of a single tan-gray fragment of tissue measuring 3
millimeters in greatest dimension, submitted entirely in A1" requires 60
some odd hours?

I have no gripe with requiring a certain degree of credentials for any
grossing which requires dissection, judgement, and knowledge of anatomy, but
this is merely counting, measuring, and transferring.  I think the CAP term
was "processing" as distinguished from "grossing."  So "processing" (in the
above sense, not in the histo sense) is gone now?

Is this coming from CAP?  Could someone give me chapter and verse so that I
can make copies and discuss it with my colleagues locally?

Thanks,

On Wed, May 19, 2010 at 1:38 PM, Mahoney,Janice A <
Janice.Mahoney@alegent.org> wrote:

> Things have changed.  All "grossing" is back to being grossing.
> Jan Mahoney
> Omaha, NE
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider
> Sent: Wednesday, May 19, 2010 12:58 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Re: Grossing Technician Qualifications
>
>  Just to clarify or perhaps cloud the picture a little more
>
> Not all grossing is "grossing."
>
> So when we're talking about transferring small biopsies, in their
> entirety, from a formalin container to a cassette, and describing the size,
> number, and color of the tissue pieces submitted, with no dissection or
> knowledge of anatomy required, that's not "grossing" in the strict sense,
> at
> least according to CAP the last time I looked, which admittedly was a
> couple
> of years ago.
>
> So what sort of educational achievements, training, or credentials are
> required for the above?
>
> Dan Schneider
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is
> faithful to the healing ministry of Jesus Christ, providing high quality
> care for the body, mind and spirit of every person.
>
> The information contained in this communication, including attachments, is
> confidential and private and intended only for the use of the addressees.
>  Unauthorized use, disclosure, distribution or copying is strictly
> prohibited and may be unlawful.  If you received this communication in
> error, please inform us of the erroneous delivery by return e-mail message
> from your computer.  Additionally, although all attachments have been
> scanned at the source for viruses, the recipient should check any
> attachments for the presence of viruses before opening.  Alegent Health
> accepts no liability for any damage caused by any virus transmitted by this
> e-mail.  Thank you for your cooperation.
>
>
From laurie.colbert <@t> huntingtonhospital.com  Wed May 19 15:00:39 2010
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Wed May 19 15:00:44 2010
Subject: [Histonet] IHC Validation (again)
Message-ID: <57BE698966D5C54EAE8612E8941D768308BCB03A@EXCHANGE3.huntingtonhospital.com>

Can anyone tell me if there is a specific question on the CAP checklist
that addresses revalidation of antibodies when starting up a new IHC
stainer?

 

Laurie Colbert

From ddreesen <@t> sbcglobal.net  Wed May 19 15:10:36 2010
From: ddreesen <@t> sbcglobal.net (Debbie Dreesen)
Date: Wed May 19 15:10:40 2010
Subject: [Histonet] alternative for bunsen burner?
In-Reply-To: <201005181737.o4IHbL5r017979@flpd119.prodigy.net>
Message-ID: <481061.10086.qm@web81003.mail.mud.yahoo.com>

Hi Brandi,
The electric three well forceps warmer that Jackie mentioned is really handy and you can find it at most medical supply companies. They sell the exact same unit but for widely different prices. It's in the Cardinal catalogue for $600 Cat. No. M7323, Mfr. No. FW-120.? Mercedes Medical sells it for $700 TRX FW120 and?American MasterTech sells their's for $625 EQFW-120.
?
Debbie Dreesen, HT(ASCP)



Message: 2
Date: Tue, 18 May 2010 11:30:11 -0400
From: Brandi Higgins 
Subject: [Histonet] alternative for bunsen burner?
To: histonet@lists.utsouthwestern.edu
Message-ID:
??? 
Content-Type: text/plain; charset=ISO-8859-1

Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they don't
have to install a gas line.? The pathologist has told me that there is some
machine that can be used instead of a flame to burn the forceps while
embedding (which is our main use for the bunsen flame).? This is the only
lab I have ever worked at and I don't know what such a machine would be
called.? If anyone knows the name, or any other alternative method can you
let me know.? Model numbers/companies would be a bonus too!? Thanks so much
for your help.

Brandi Higgins, BS, HT(ASCP)
From thomas.crowell <@t> novartis.com  Wed May 19 15:26:52 2010
From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com)
Date: Wed May 19 15:27:01 2010
Subject: [Histonet] Thomas Crowell is out of the office.
Message-ID: 


I will be out of the office starting  05/19/2010 and will not return until
05/25/2010.

Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.
From liz <@t> premierlab.com  Wed May 19 15:41:36 2010
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Wed May 19 15:42:02 2010
Subject: [Histonet] IHC Validation (again)
In-Reply-To: <57BE698966D5C54EAE8612E8941D768308BCB03A@EXCHANGE3.huntingtonhospital.com>
Message-ID: 

Laurie

I'm not aware of a particular question, but I would believe you would
have to perform some validation steps for each antibody.  I would
approach it the same way you approach validating new lots of antisera.
The CAP paper on standardization of IHC recommends 25 different samples
when you initially validate an antibody - 10 samples that have high
levels of target antigen, 10 intermediate to low levels and 5 negative.
To revalidate new lots they recommend only 3 tissue samples - 1 high, 1
med to low, and 1 negative.  Granted this only applies to routine
markers.  For prognostic markers such as ER/PR and Her2 then additional
samples need to be tested - there are new guidelines for ER/ER out and
the new recommendations for validation for ER/ER are briefly reviewed in
the CAP Today April issue.  Guidelines for validation of ER/PR will be
published in the June issue of Archives of Pathology and Laboratory
Medicine.

Liz 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Wednesday, May 19, 2010 2:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation (again)

Can anyone tell me if there is a specific question on the CAP checklist
that addresses revalidation of antibodies when starting up a new IHC
stainer?

 

Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From burch007 <@t> mc.duke.edu  Wed May 19 16:04:46 2010
From: burch007 <@t> mc.duke.edu (James L Burchette)
Date: Wed May 19 16:04:57 2010
Subject: [Histonet] Freezing spray artifact
In-Reply-To: <379A927A452F3D43A3C8705F4E67905F0FC1ECB56C@EX05.net.ucsf.edu>
Message-ID: 

Erin - 

Try this trick when using the freezing spray: take a folded kim wipe 
tissue and fold it again, then again then the long fold is folded twice so 
you end up with roughly a 1 inch by 1 inch multi layer slide wiper. Run 
your finger down each crease and after the final fold, give it a little 
pinch and twist so it holds its shape. You are probably wondering what 
does this have to do with freezing spray artifact... Dip the square of Kim 
Wipe in your water bath and press it on the cut surface of your tissue 
block. It will act as an insulator when you spray the block surface. They 
are also good for soaking the face of a block and are better than a wad of 
tissue when wiping slides.

JB





"Martin, Erin"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/19/2010 12:41 PM

To
histonet 
cc

Subject
[Histonet] Freezing spray artifact






Has anyone run into a problem or artifact from freezing spray?  I think we 
may be having a problem with it but I can't find any pictures or 
descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From sgoebel <@t> xbiotech.com  Wed May 19 16:54:07 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Wed May 19 16:54:11 2010
Subject: [Histonet] Equipment Repair Dude
Message-ID: <20100519145407.9e2d9aa830e8449a2412eb1e4f2f067e.7f63da1337.wbe@email04.secureserver.net>


   Hey ya'll,

   I'm  in the Austin Texas area and am in need    fix  a  cryostat  and  a  microtome  (or  at  least com dia   problem).   Does  anyone out there know of someone who could c   and  work on some of my equipment that won't charge the cost of just    replacing it?

   Thanks!!

   Sarah Goebel,
   Histotechnician
   
   XBiotech USA Inc.

   <
   8201 East Riverside Dr. Bldg 4 Suite 100

   Austin, Texas  78744

   <
   (512)386-5107
From napoli <@t> siscom.net  Wed May 19 17:08:03 2010
From: napoli <@t> siscom.net (Andrew Burgeson)
Date: Wed May 19 17:08:12 2010
Subject: [Histonet] 
	AANLkTik8dnsQt16RPMSS8_x5fgqQE_18-ctUeNp71V0L@mail.gmail.com
Message-ID: <4bf46143.268.24d1.1221708583@siscom.net>

"One thing i forgot to mention wasthat when you embed, try
to orientate the
tissue so that the long axis (if there is one) lies in the
same direction as
the cutting stroke. when embedding, orientate the tissue at
a slight
diagonal, so that the knife dous not continously pass
through the tissue on
the cutting stroke - 

(this works well for skins also, except make sure the
dermis is away from the knife)


I do not agree with the above statement about the "dermis
being embedded so as to be facing away from the blade." The
last tissue to hit the knife edge should be EPIDERMIS.
Dermis and SubQ fat should be the first tissues to hit the
blade. Perhaps this is what you meant by "dermis?"
Otherwise, I would agree with that methodology of
orientation and angle.

MethylMethacrylate bone embedding works very well from what
I understand. See link:

http://www.jhc.org/cgi/content/full/45/2/307 



From raj <@t> bluemarble.net  Wed May 19 17:09:29 2010
From: raj <@t> bluemarble.net (Rebecca Johnson)
Date: Wed May 19 17:10:01 2010
Subject: [Histonet] Histonet
Message-ID: 

Does anyone have Peggy Wenk email or phone.
Thanks


From napoli <@t> siscom.net  Wed May 19 17:20:47 2010
From: napoli <@t> siscom.net (Andrew Burgeson)
Date: Wed May 19 17:20:54 2010
Subject: [Histonet] bone processing
Message-ID: <4bf4643f.238.2918.1412147363@siscom.net>

I have seen a lot of traffic on the site regarding bone
processing...If anyone is interested, I have personally
witnessed the following system being used very successfully
for the embedding and sectioning of bone, including bone
that has been surgically implanted with metal devices.

I just thought I would give this company's product(s) a
"plug" because I know that the "EXAKT System" works
extremely well for certain applications.

Anyone interested click the link below: (worth a look)


http://www.exaktusa.com/applications/

AB

From rsrichmond <@t> gmail.com  Wed May 19 20:00:55 2010
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Wed May 19 20:00:58 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
Message-ID: 

Thanks to all who've been most helpful.

I probably shouldn't be admitting it, but I don't think I had enough
college science courses to be allowed to gross today. Maybe if they
don't find out that two of my biology courses were in paleontology. I
can gross a trilobite like you wouldn't believe!

Bob Richmond
Samurai Pathologist
Konxville TN

From JMacDonald <@t> mtsac.edu  Wed May 19 23:55:37 2010
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Wed May 19 23:55:46 2010
Subject: [Histonet] Freezing spray artifact
In-Reply-To: <8C023B4AB999614BA4791BAEB26E2738399E63@UWHC-MAIL01.uwhis.hosp.wisc.edu>
Message-ID: 

We also had the appearance of burnt tissue when someone was using the 
spray excessively.




"Sebree Linda A"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/19/2010 11:07 AM

To
"Martin, Erin" , "histonet" 

cc

Subject
RE: [Histonet] Freezing spray artifact






Erin, 
One can certainly get cracking of the tissue and surrounding paraffin
from a too intense application of freezing spray.  This is usually
grossly visible, at least in the paraffin. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin,
Erin
Sent: Wednesday, May 19, 2010 11:09 AM
To: histonet
Subject: [Histonet] Freezing spray artifact

Has anyone run into a problem or artifact from freezing spray?  I think
we may be having a problem with it but I can't find any pictures or
descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From tjay30 <@t> yahoo.com  Thu May 20 00:51:59 2010
From: tjay30 <@t> yahoo.com (Tim)
Date: Thu May 20 00:52:02 2010
Subject: [Histonet] PT tech in Santa Rosa
Message-ID: <212551.8075.qm@web34307.mail.mud.yahoo.com>

I'm still looking for a p/t tech to run a new GI path lab in Santa Rosa. Send resumes to tjay30@yahoo.com. Great facility, flexible hours, friendly docs, warm, caring staff, and competitive pay.  

Timothy Garcia-Jay
Pillar Consulting






      

From Aidan.Schurr <@t> leica-microsystems.com  Thu May 20 01:15:24 2010
From: Aidan.Schurr <@t> leica-microsystems.com (Aidan Schurr)
Date: Thu May 20 01:15:48 2010
Subject: [Histonet] unsubscribe
In-Reply-To: 
Message-ID: <625858D937841B4D89752F7B6C359849043DDD21@romba.vsl.com.au>

 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Thursday, 20 May 2010 3:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 78, Issue 28

Send Histonet mailing list submissions to
	histonet@lists.utsouthwestern.edu

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When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."


Today's Topics:

   1. RE: Freezing spray artifact (Shirley A. Powell)
   2. RE: IHC Validation on new instrument (Liz Chlipala)
   3. Re: Freezing spray artifact (Rena Fail)
   4. RE: Freezing spray artifact (Sebree Linda A)
   5. IHC Validation on microwaved tissue (Matt Brooks)
   6. charging for cytospins (Tench, Bill)
   7. Re: Re: Grossing Technician Qualifications (Daniel Schneider)
   8. Temp Histo Tech Needed (Marcia Fisher)
   9. IHC Validation on new instrument (Troutman, Kenneth A)
  10. PPE (Cindy DeRiso)
  11. RE: Freezing spray artifact (sgoebel@xbiotech.com)
  12. RE: Re: Grossing Technician Qualifications (Mahoney,Janice A)
  13. RE: PPE (Liz Chlipala)
  14. RE: PPE (Sherwood, Margaret )
  15. Re: Re: Grossing Technician Qualifications (Daniel Schneider)
  16. IHC Validation (again) (Laurie Colbert)
  17. Re: alternative for bunsen burner? (Debbie Dreesen)
  18. Thomas Crowell is out of the office. (thomas.crowell@novartis.com)
  19. RE: IHC Validation (again) (Liz Chlipala)
  20. Re: Freezing spray artifact (James L Burchette)
  21. Equipment Repair Dude (sgoebel@xbiotech.com)
  22. 	AANLkTik8dnsQt16RPMSS8_x5fgqQE_18-ctUeNp71V0L@mail.gmail.com
      (Andrew Burgeson)
  23. Histonet (Rebecca Johnson)
  24. bone processing (Andrew Burgeson)
  25. Re: Grossing Technician Qualifications (Robert Richmond)
  26. RE: Freezing spray artifact (Jennifer MacDonald)


----------------------------------------------------------------------

Message: 1
Date: Wed, 19 May 2010 13:15:37 -0400
From: "Shirley A. Powell" 
Subject: [Histonet] RE: Freezing spray artifact
To: "Martin, Erin" , histonet
	
Message-ID:
	<9BF995BC0E47744E9673A41486E24EE2268C5518AA@MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="us-ascii"

There is a text written by the late Lee Luna and Samuel Wesley Thompson entitled An Atlas of Artifacts in this the artifact from freeze spray is pictured.  The ISBN # is 0-398-03624-1 Published by Charles C. Thomas, Publisher.  

Shirley Powell

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Wednesday, May 19, 2010 12:09 PM
To: histonet
Subject: [Histonet] Freezing spray artifact

Has anyone run into a problem or artifact from freezing spray?  I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 2
Date: Wed, 19 May 2010 11:21:32 -0600
From: "Liz Chlipala" 
Subject: RE: [Histonet] IHC Validation on new instrument
To: "Laurie Colbert" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

We perform our entire validation process as a new piece of equipment.
Our validation protocols are quite extensive, up to about 85 pages long on each piece of major equipment, at least that's what it was for our new prisma stainer and glass coverslipper.  We perform an installation/operational qualification protocol or an IOQ.  If we move the instrument we also do the same thing, we already have the protocol written which takes most of the time we just execute it again.  We are a GLP lab so we work off a Validation Master Plan that basically tells us how we are going to validate each piece of equipment in the lab.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Wednesday, May 19, 2010 9:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation on new instrument

What do others do when validating a new model of a piece of equipment - same manufacturer, same basic staining process, but an updated version of the equipment?

 

I've been told the protocols should be the same and that we only need to run three controls with three different but similar protocols to determine what looks best.  Do you all think that is thorough enough, or would you run actual patient cases and compare old and new equipment?  I don't see where the CAP checklist refers to new equipment - just new antibodies and new antibody lots.

 

Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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------------------------------

Message: 3
Date: Wed, 19 May 2010 10:30:49 -0700 (PDT)
From: Rena Fail 
Subject: Re: [Histonet] Freezing spray artifact
To: "Martin, Erin" ,	histonet
	
Message-ID: <96641.83001.qm@web180301.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Erin,
Holding the can too close and/or spraying for a prolonged period both cause freeze artifact. Look at your block, it will not have a smooth look to the paraffin.?it will?have lines it much like a cracked piece of glass. the tissue will appear cracked on the waterbath and will even separate along these lines.?The cracks can be seen microscopically amd interfere with the architecture?of the tissue. 
Rena Fail



?----- Original Message ----
From: "Martin, Erin" 
To: histonet 
Sent: Wed, May 19, 2010 12:09:22 PM
Subject: [Histonet] Freezing spray artifact

Has anyone run into a problem or artifact from freezing spray?? I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 4
Date: Wed, 19 May 2010 12:38:32 -0500
From: "Sebree Linda A" 
Subject: RE: [Histonet] Freezing spray artifact
To: "Martin, Erin" ,	"histonet"
	
Message-ID:
	<8C023B4AB999614BA4791BAEB26E2738399E63@UWHC-MAIL01.uwhis.hosp.wisc.edu>
	
Content-Type: text/plain;	charset="US-ASCII"

Erin,
One can certainly get cracking of the tissue and surrounding paraffin from a too intense application of freezing spray.  This is usually grossly visible, at least in the paraffin. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Wednesday, May 19, 2010 11:09 AM
To: histonet
Subject: [Histonet] Freezing spray artifact

Has anyone run into a problem or artifact from freezing spray?  I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 5
Date: Wed, 19 May 2010 10:44:40 -0700
From: "Matt Brooks" 
Subject: [Histonet] IHC Validation on microwaved tissue
To: 
Message-ID:
	<706224670091FE47997AEF88EFADE7CA01550C9A@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>
	
Content-Type: text/plain;	charset="us-ascii"

Hello All,

We have new microwave tissue processor, until this purchase all tissue has been placed on a standard VIP processor.  My medical director would like for most of our 125 antibodies worked up on microwave processed tissue.  I have been challenged with getting specimens to process on the instrument for IHC antibody validation purposes.  Initially we will be processing GI biopsy specimens, but we will eventually progress to other biopsy tissues and so on.  I have had success getting some colon cancer cases, some tonsil and other tissue. We will be mainly performing H.
pylori, which is hard to get a large enough specimen to obtain a sample for this purpose.  Anyway I was wondering how other labs are handling validation in this situation.  Thank you for your time in this matter. 

Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
mbrooks@incytepathology.com
509-892-2744




------------------------------

Message: 6
Date: Wed, 19 May 2010 10:52:21 -0700
From: "Tench, Bill" 
Subject: [Histonet] charging for cytospins
To: histonet@lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863103@MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii

Can you charge for two different stains for the urine cytospin?

 

The answer to this question is a "depends."  If you are just doing any of the "optional" stains that may be used on a cytology preparation (namely Pap, H&E, romanovsky) you are NOT permitted to charge for each of these (they are not considered "special stains").  If you did an iron stain, or melanin stain, or mucin stain, you could charge separately for these "special" stains.

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

Bill.Tench@pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


mail2.pph.org made the following annotations
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------------------------------

Message: 7
Date: Wed, 19 May 2010 12:57:55 -0500
From: Daniel Schneider 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

Just to clarify or perhaps cloud the picture a little more

Not all grossing is "grossing."

So when we're talking about transferring small biopsies, in their entirety, from a formalin container to a cassette, and describing the size, number, and color of the tissue pieces submitted, with no dissection or knowledge of anatomy required, that's not "grossing" in the strict sense, at least according to CAP the last time I looked, which admittedly was a couple of years ago.

So what sort of educational achievements, training, or credentials are required for the above?

Dan Schneider


------------------------------

Message: 8
Date: Wed, 19 May 2010 10:57:39 -0700
From: "Marcia Fisher" 
Subject: [Histonet] Temp Histo Tech Needed
To: 
Message-ID:
	<3ACBB5D73A417547A01970CD3EB55093047B626B@MAIL1.ecrmc.ci.el-centro.ca.us>
	
Content-Type: text/plain; charset="iso-8859-1"

We are in need of a temporary, ASCP certified histotech for 4-6 weeks beginning June 21 at El Centro Regional Medical Center.  Located just 2 hours east of San Diego, 100 miles south of Palm Springs and only 60 miles west of Yuma.  Our new lab was just completed in February, 2010 and is state-of-the-art. 

El Centro Regional Medical Center is a nonprofit, community based hospital owned by the City of El Centro. For over 50 years, ECRMC has provided medical care to the Imperial Valley and has become a leading healthcare system as an excellent source for health care services.

In addition to the medical center, the community is served by two Outpatient Centers, a Wound Healing Center, a Children's Specialist Clinic and various educational programs including Asthma and Diabetes.

With more than 150 accredited physicians and over 900 employees, El Centro Regional Medical Center strives for excellence in the Imperial Valley.

Please contact me thru work email if interested and more details.   mfisher@ecrmc.org

M. Fisher

El Centro Regional Medical Center

1415 Ross Ave
El Centro, CA  92243
760-339-7267
760-482-5365(F)
www.ecrmc.org  
 
Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments.

________________________________

   

------------------------------

Message: 9
Date: Wed, 19 May 2010 13:06:27 -0500
From: "Troutman, Kenneth A" 
Subject: [Histonet] IHC Validation on new instrument
To: "Histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<7B310892042DA74CB3590053F424CFE60B83F97011@ITS-HCWNEM06.ds.Vanderbilt.edu>
	
Content-Type: text/plain; charset="iso-8859-1"


Hi Laurie,

I have a Benchmark Ultra and a Benchmark XT from Ventana and they follow the basic steps, similar protocols and I needed to revalidate everything.  They are sufficiently different (in my experience) that it warranted a complete revalidation.  A part of the reasoning for this was some of the bulk reagents were different (a consideration that may lead you to not completely revalidate).  For me, there were a few protocols that were quite different from one to the other.  I would go to the trouble up front, that way there is no question later.

Good luck!

Ashley Troutman BS, HT(ASCP) QIHC
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4532
Nashville, TN  37232
615-343-9134

Message: 4
Date: Wed, 19 May 2010 07:59:30 -0700
From: "Laurie Colbert" 
Subject: [Histonet] IHC Validation on new instrument
To: 
Message-ID:
        <57BE698966D5C54EAE8612E8941D768308BCAF81@EXCHANGE3.huntingtonhospital.com>

Content-Type: text/plain;       charset="us-ascii"

What do others do when validating a new model of a piece of equipment - same manufacturer, same basic staining process, but an updated version of the equipment?



I've been told the protocols should be the same and that we only need to run three controls with three different but similar protocols to determine what looks best.  Do you all think that is thorough enough, or would you run actual patient cases and compare old and new equipment?  I don't see where the CAP checklist refers to new equipment - just new antibodies and new antibody lots.



Laurie Colbert


------------------------------

Message: 10
Date: Wed, 19 May 2010 14:20:42 -0400
From: Cindy DeRiso 
Subject: [Histonet] PPE
To: Histonet@lists.utsouthwestern.edu
Message-ID: <4BF42BFA.6030006@yale.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Does any one use or require gloves when cutting and embedding? How about safety goggles when embedding?
Thanks in advance-
Cindy DeRiso
Yale University Pathology



------------------------------

Message: 11
Date: Wed, 19 May 2010 11:37:30 -0700
From: sgoebel@xbiotech.com
Subject: RE: [Histonet] Freezing spray artifact
To: "Martin,Erin" 
Cc: histonet 
Message-ID:
	<20100519113730.9e2d9aa830e8449a2412eb1e4f2f067e.24d0cf7c87.wbe@email04.secureserver.net>
	
Content-Type: text/plain; charset="utf-8"


   [DEL: 3D"" :DEL] 
   Picture  on the  left has the artifact, picture on the right is good
   to go.  Hope this
   Sarah Goebel, B.A., HT (ASCP)
   Histotechnician
   <   XBiotech USA Inc.
   8201 Eas      (512)386-51
   -------- Original Message --------
   Subject: [Histonet] Freezing spray artifact
   From: "Martin, Erin" 
   Date: Wed, May 19, 2010 9:09 am
   To: histonet 
   Has anyone run into a problem or artifact from freezing spray? I think
   we     descriptio   Thanks in advance,
   Erin
   Erin Martin, Histology Supervisor
   UCSF Department of Dermatopathology
   415-353-7248
   _______________________________________________
   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet

References

   1. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"


------------------------------

Message: 12
Date: Wed, 19 May 2010 13:38:43 -0500
From: "Mahoney,Janice A" 
Subject: RE: [Histonet] Re: Grossing Technician Qualifications
To: 'Daniel Schneider' ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A1B6@EXCHMBC2.ad.ah.local>
Content-Type: text/plain; charset="us-ascii"

Things have changed.  All "grossing" is back to being grossing.
Jan Mahoney
Omaha, NE

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider
Sent: Wednesday, May 19, 2010 12:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Grossing Technician Qualifications

Just to clarify or perhaps cloud the picture a little more

Not all grossing is "grossing."

So when we're talking about transferring small biopsies, in their
entirety, from a formalin container to a cassette, and describing the size,
number, and color of the tissue pieces submitted, with no dissection or
knowledge of anatomy required, that's not "grossing" in the strict sense, at
least according to CAP the last time I looked, which admittedly was a couple
of years ago.

So what sort of educational achievements, training, or credentials are
required for the above?

Dan Schneider
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person.

The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees.  Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.  If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.  Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening.  Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.  Thank you for your cooperation.




------------------------------

Message: 13
Date: Wed, 19 May 2010 13:15:08 -0600
From: "Liz Chlipala" 
Subject: RE: [Histonet] PPE
To: ,	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

I require gloves when sectioning only because it helps eliminate
epithelial floaters, with respects to embedding the tech can choose to
or not to use gloves.  No safety goggles are required.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy
DeRiso
Sent: Wednesday, May 19, 2010 12:21 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] PPE

Does any one use or require gloves when cutting and embedding? How about

safety goggles when embedding?
Thanks in advance-
Cindy DeRiso
Yale University Pathology

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 14
Date: Wed, 19 May 2010 15:16:44 -0400
From: "Sherwood, Margaret " 
Subject: RE: [Histonet] PPE
To: ,	
Message-ID:
	<073AE2BEA1C2BA4A8837AB6C4B943D9703E242FF@PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"

We wear gloves when handling tissue and embedding.  No gloves for sectioning and
no safety goggles.   

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DeRiso
Sent: Wednesday, May 19, 2010 2:21 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] PPE

Does any one use or require gloves when cutting and embedding? How about 
safety goggles when embedding?
Thanks in advance-
Cindy DeRiso
Yale University Pathology

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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but does not contain patient information, please contact the sender and properly
dispose of the e-mail.




------------------------------

Message: 15
Date: Wed, 19 May 2010 14:18:46 -0500
From: Daniel Schneider 
Subject: Re: [Histonet] Re: Grossing Technician Qualifications
To: "Mahoney,Janice A" 
Cc: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

Really. Are you sure?

"Received in formalin, labeled with the patient's name and number, the
specimen consists of a single tan-gray fragment of tissue measuring 3
millimeters in greatest dimension, submitted entirely in A1" requires 60
some odd hours?

I have no gripe with requiring a certain degree of credentials for any
grossing which requires dissection, judgement, and knowledge of anatomy, but
this is merely counting, measuring, and transferring.  I think the CAP term
was "processing" as distinguished from "grossing."  So "processing" (in the
above sense, not in the histo sense) is gone now?

Is this coming from CAP?  Could someone give me chapter and verse so that I
can make copies and discuss it with my colleagues locally?

Thanks,

On Wed, May 19, 2010 at 1:38 PM, Mahoney,Janice A <
Janice.Mahoney@alegent.org> wrote:

> Things have changed.  All "grossing" is back to being grossing.
> Jan Mahoney
> Omaha, NE
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider
> Sent: Wednesday, May 19, 2010 12:58 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Re: Grossing Technician Qualifications
>
>  Just to clarify or perhaps cloud the picture a little more
>
> Not all grossing is "grossing."
>
> So when we're talking about transferring small biopsies, in their
> entirety, from a formalin container to a cassette, and describing the size,
> number, and color of the tissue pieces submitted, with no dissection or
> knowledge of anatomy required, that's not "grossing" in the strict sense,
> at
> least according to CAP the last time I looked, which admittedly was a
> couple
> of years ago.
>
> So what sort of educational achievements, training, or credentials are
> required for the above?
>
> Dan Schneider
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is
> faithful to the healing ministry of Jesus Christ, providing high quality
> care for the body, mind and spirit of every person.
>
> The information contained in this communication, including attachments, is
> confidential and private and intended only for the use of the addressees.
>  Unauthorized use, disclosure, distribution or copying is strictly
> prohibited and may be unlawful.  If you received this communication in
> error, please inform us of the erroneous delivery by return e-mail message
> from your computer.  Additionally, although all attachments have been
> scanned at the source for viruses, the recipient should check any
> attachments for the presence of viruses before opening.  Alegent Health
> accepts no liability for any damage caused by any virus transmitted by this
> e-mail.  Thank you for your cooperation.
>
>


------------------------------

Message: 16
Date: Wed, 19 May 2010 13:00:39 -0700
From: "Laurie Colbert" 
Subject: [Histonet] IHC Validation (again)
To: 
Message-ID:
	<57BE698966D5C54EAE8612E8941D768308BCB03A@EXCHANGE3.huntingtonhospital.com>
	
Content-Type: text/plain;	charset="us-ascii"

Can anyone tell me if there is a specific question on the CAP checklist
that addresses revalidation of antibodies when starting up a new IHC
stainer?

 

Laurie Colbert



------------------------------

Message: 17
Date: Wed, 19 May 2010 13:10:36 -0700 (PDT)
From: Debbie Dreesen 
Subject: Re: [Histonet] alternative for bunsen burner?
To: histonet@lists.utsouthwestern.edu
Message-ID: <481061.10086.qm@web81003.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Brandi,
The electric three well forceps warmer that Jackie mentioned is really handy and you can find it at most medical supply companies. They sell the exact same unit but for widely different prices. It's in the Cardinal catalogue for $600 Cat. No. M7323, Mfr. No. FW-120.? Mercedes Medical sells it for $700 TRX FW120 and?American MasterTech sells their's for $625 EQFW-120.
?
Debbie Dreesen, HT(ASCP)



Message: 2
Date: Tue, 18 May 2010 11:30:11 -0400
From: Brandi Higgins 
Subject: [Histonet] alternative for bunsen burner?
To: histonet@lists.utsouthwestern.edu
Message-ID:
??? 
Content-Type: text/plain; charset=ISO-8859-1

Hello all,

Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they don't
have to install a gas line.? The pathologist has told me that there is some
machine that can be used instead of a flame to burn the forceps while
embedding (which is our main use for the bunsen flame).? This is the only
lab I have ever worked at and I don't know what such a machine would be
called.? If anyone knows the name, or any other alternative method can you
let me know.? Model numbers/companies would be a bonus too!? Thanks so much
for your help.

Brandi Higgins, BS, HT(ASCP)


------------------------------

Message: 18
Date: Wed, 19 May 2010 16:26:52 -0400
From: thomas.crowell@novartis.com
Subject: [Histonet] Thomas Crowell is out of the office.
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
	
Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting  05/19/2010 and will not return until
05/25/2010.

Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.

------------------------------

Message: 19
Date: Wed, 19 May 2010 14:41:36 -0600
From: "Liz Chlipala" 
Subject: RE: [Histonet] IHC Validation (again)
To: "Laurie Colbert" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Laurie

I'm not aware of a particular question, but I would believe you would
have to perform some validation steps for each antibody.  I would
approach it the same way you approach validating new lots of antisera.
The CAP paper on standardization of IHC recommends 25 different samples
when you initially validate an antibody - 10 samples that have high
levels of target antigen, 10 intermediate to low levels and 5 negative.
To revalidate new lots they recommend only 3 tissue samples - 1 high, 1
med to low, and 1 negative.  Granted this only applies to routine
markers.  For prognostic markers such as ER/PR and Her2 then additional
samples need to be tested - there are new guidelines for ER/ER out and
the new recommendations for validation for ER/ER are briefly reviewed in
the CAP Today April issue.  Guidelines for validation of ER/PR will be
published in the June issue of Archives of Pathology and Laboratory
Medicine.

Liz 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Wednesday, May 19, 2010 2:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation (again)

Can anyone tell me if there is a specific question on the CAP checklist
that addresses revalidation of antibodies when starting up a new IHC
stainer?

 

Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 20
Date: Wed, 19 May 2010 17:04:46 -0400
From: James L Burchette 
Subject: Re: [Histonet] Freezing spray artifact
To: "Martin, Erin" 
Cc: histonet ,
	histonet-bounces@lists.utsouthwestern.edu
Message-ID:
	
	
Content-Type: text/plain; charset="US-ASCII"

Erin - 

Try this trick when using the freezing spray: take a folded kim wipe 
tissue and fold it again, then again then the long fold is folded twice so 
you end up with roughly a 1 inch by 1 inch multi layer slide wiper. Run 
your finger down each crease and after the final fold, give it a little 
pinch and twist so it holds its shape. You are probably wondering what 
does this have to do with freezing spray artifact... Dip the square of Kim 
Wipe in your water bath and press it on the cut surface of your tissue 
block. It will act as an insulator when you spray the block surface. They 
are also good for soaking the face of a block and are better than a wad of 
tissue when wiping slides.

JB





"Martin, Erin"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/19/2010 12:41 PM

To
histonet 
cc

Subject
[Histonet] Freezing spray artifact






Has anyone run into a problem or artifact from freezing spray?  I think we 
may be having a problem with it but I can't find any pictures or 
descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 21
Date: Wed, 19 May 2010 14:54:07 -0700
From: sgoebel@xbiotech.com
Subject: [Histonet] Equipment Repair Dude
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<20100519145407.9e2d9aa830e8449a2412eb1e4f2f067e.7f63da1337.wbe@email04.secureserver.net>
	
Content-Type: text/plain; charset="utf-8"


   Hey ya'll,

   I'm  in the Austin Texas area and am in need    fix  a  cryostat  and  a  microtome  (or  at  least com dia   problem).   Does  anyone out there know of someone who could c   and  work on some of my equipment that won't charge the cost of just    replacing it?

   Thanks!!

   Sarah Goebel,
   Histotechnician
   
   XBiotech USA Inc.

   <
   8201 East Riverside Dr. Bldg 4 Suite 100

   Austin, Texas  78744

   <
   (512)386-5107


------------------------------

Message: 22
Date: Wed, 19 May 2010 18:08:03 -0400
From: "Andrew Burgeson" 
Subject: [Histonet]
	AANLkTik8dnsQt16RPMSS8_x5fgqQE_18-ctUeNp71V0L@mail.gmail.com
To: Histonet@lists.utsouthwestern.edu
Message-ID: <4bf46143.268.24d1.1221708583@siscom.net>
Content-Type: text/plain; charset="iso-8859-1"

"One thing i forgot to mention wasthat when you embed, try
to orientate the
tissue so that the long axis (if there is one) lies in the
same direction as
the cutting stroke. when embedding, orientate the tissue at
a slight
diagonal, so that the knife dous not continously pass
through the tissue on
the cutting stroke - 

(this works well for skins also, except make sure the
dermis is away from the knife)


I do not agree with the above statement about the "dermis
being embedded so as to be facing away from the blade." The
last tissue to hit the knife edge should be EPIDERMIS.
Dermis and SubQ fat should be the first tissues to hit the
blade. Perhaps this is what you meant by "dermis?"
Otherwise, I would agree with that methodology of
orientation and angle.

MethylMethacrylate bone embedding works very well from what
I understand. See link:

http://www.jhc.org/cgi/content/full/45/2/307 





------------------------------

Message: 23
Date: Wed, 19 May 2010 17:09:29 -0500
From: "Rebecca Johnson" 
Subject: [Histonet] Histonet
To: "histonet" 
Message-ID: 
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Does anyone have Peggy Wenk email or phone.
Thanks




------------------------------

Message: 24
Date: Wed, 19 May 2010 18:20:47 -0400
From: "Andrew Burgeson" 
Subject: [Histonet] bone processing
To: histonet@lists.utsouthwestern.edu
Message-ID: <4bf4643f.238.2918.1412147363@siscom.net>
Content-Type: text/plain; charset="iso-8859-1"

I have seen a lot of traffic on the site regarding bone
processing...If anyone is interested, I have personally
witnessed the following system being used very successfully
for the embedding and sectioning of bone, including bone
that has been surgically implanted with metal devices.

I just thought I would give this company's product(s) a
"plug" because I know that the "EXAKT System" works
extremely well for certain applications.

Anyone interested click the link below: (worth a look)


http://www.exaktusa.com/applications/

AB



------------------------------

Message: 25
Date: Wed, 19 May 2010 21:00:55 -0400
From: Robert Richmond 
Subject: [Histonet] Re: Grossing Technician Qualifications
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

Thanks to all who've been most helpful.

I probably shouldn't be admitting it, but I don't think I had enough
college science courses to be allowed to gross today. Maybe if they
don't find out that two of my biology courses were in paleontology. I
can gross a trilobite like you wouldn't believe!

Bob Richmond
Samurai Pathologist
Konxville TN



------------------------------

Message: 26
Date: Wed, 19 May 2010 21:55:37 -0700
From: Jennifer MacDonald 
Subject: RE: [Histonet] Freezing spray artifact
To: "Sebree Linda A" 
Cc: histonet ,
	histonet-bounces@lists.utsouthwestern.edu, "Martin,	Erin"
	
Message-ID:
	
Content-Type: text/plain; charset="US-ASCII"

We also had the appearance of burnt tissue when someone was using the 
spray excessively.




"Sebree Linda A"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/19/2010 11:07 AM

To
"Martin, Erin" , "histonet" 

cc

Subject
RE: [Histonet] Freezing spray artifact






Erin, 
One can certainly get cracking of the tissue and surrounding paraffin
from a too intense application of freezing spray.  This is usually
grossly visible, at least in the paraffin. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin,
Erin
Sent: Wednesday, May 19, 2010 11:09 AM
To: histonet
Subject: [Histonet] Freezing spray artifact

Has anyone run into a problem or artifact from freezing spray?  I think
we may be having a problem with it but I can't find any pictures or
descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 78, Issue 28
****************************************

From louise.renton <@t> gmail.com  Thu May 20 02:28:39 2010
From: louise.renton <@t> gmail.com (louise renton)
Date: Thu May 20 02:29:15 2010
Subject: [Histonet]
	AANLkTik8dnsQt16RPMSS8_x5fgqQE_18-ctUeNp71V0L@mail.gmail.com
In-Reply-To: <4bf46143.268.24d1.1221708583@siscom.net>
References: <4bf46143.268.24d1.1221708583@siscom.net>
Message-ID: 

My wrong....I meant epidermis. Blame old age




On Thu, May 20, 2010 at 12:08 AM, Andrew Burgeson  wrote:

> "One thing i forgot to mention wasthat when you embed, try
> to orientate the
> tissue so that the long axis (if there is one) lies in the
> same direction as
> the cutting stroke. when embedding, orientate the tissue at
> a slight
> diagonal, so that the knife dous not continously pass
> through the tissue on
> the cutting stroke -
>
> (this works well for skins also, except make sure the
> dermis is away from the knife)
>
>
> I do not agree with the above statement about the "dermis
> being embedded so as to be facing away from the blade." The
> last tissue to hit the knife edge should be EPIDERMIS.
> Dermis and SubQ fat should be the first tissues to hit the
> blade. Perhaps this is what you meant by "dermis?"
> Otherwise, I would agree with that methodology of
> orientation and angle.
>
> MethylMethacrylate bone embedding works very well from what
> I understand. See link:
>
> http://www.jhc.org/cgi/content/full/45/2/307
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
From sbreeden <@t> nmda.nmsu.edu  Thu May 20 07:26:40 2010
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Thu May 20 07:26:45 2010
Subject: [Histonet] Linda Blazek
Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E470C8@nmdamailsvr.nmda.ad.nmsu.edu>

Please contact me at this email address.  Thank you.

 

Sally Breeden, HT(ASCP)

Veterinary Diagnostic Services

New Mexico Department of Agriculture

700 Camino de Salud NE

Albuquerque, NM  87108

595-841-2576

 

From rjp226 <@t> cornell.edu  Thu May 20 07:35:06 2010
From: rjp226 <@t> cornell.edu (Richard Pattison)
Date: Thu May 20 07:35:18 2010
Subject: [Histonet] rfp in cryosections
Message-ID: <4BF52C7A.5010703@cornell.edu>

Hello,
I am currently making cryosections of transgenic tomato fruit expressing 
mRFP 
 
but am having some problems visualising fluorescence with a confocal. I 
realise fluorescent proteins work best in live cells but I have read a 
few reports of direct GFP detection in fixed cryosections.  When I view 
hand sections of the fruit I can easily see the flourescence with no 
autofluorescence in my wild type control. However, with my cryosections 
the autofluorescence increases and the RFP fluorescence is so diminished 
i can't distinguish it. I've tried three different fixatives (in order 
of most to least autofluorescence: 4% formaldehyde/0.25% glutaraldehyde 
in PBS, 3:1 ethanol:acetic acid and 1:3 ethanol:acetic acid), followed 
by infiltration with 20% sucrose in PBS. I embed in OCT and use the 
cryojane tape transfer system to transfer the sections to slides. After 
sectioning at 14 microns I dry the slides at room temperature before 
viewing. I assume some part of the fixation process (and/or the cryojane 
tape transfer process) is leading to autofluorescence in the RFP range. 
Does anyone have any suggestions for minimizing this?
Thanks in advance,
Richard

-- 
Richard Pattison
Boyce Thompson Institute for Plant Research
Tower Road
Ithaca, NY, 14853-1801
USA
Phone: +1 607 254 8757
rjp226@cornell.edu
http://bti.cornell.edu/CarmenCatala.php

From bill501 <@t> mindspring.com  Thu May 20 07:35:37 2010
From: bill501 <@t> mindspring.com (Bill B.)
Date: Thu May 20 07:35:54 2010
Subject: [Histonet] Re: Grossing Technician Qualifications
In-Reply-To: 
References: 
Message-ID: 

At 9:00 PM -0400 5/19/10, Robert Richmond wrote:
>I probably shouldn't be admitting it, but I don't think I had enough
>college science courses to be allowed to gross today. Maybe if they
>don't find out that two of my biology courses were in paleontology. I
>can gross a trilobite like you wouldn't believe!

Hah!! If it is defined as college, I took minimal premed courses and majored in classics and literature. I guess I cannot gross either. My attitude is screw the pundits. I will supervise in my own way. It is what 'professional' means. 

BB


From holmberg.katie <@t> gmail.com  Thu May 20 07:44:53 2010
From: holmberg.katie <@t> gmail.com (katie holmberg)
Date: Thu May 20 07:45:22 2010
Subject: [Histonet] (no subject)
Message-ID: 


From b-frederick <@t> northwestern.edu  Thu May 20 08:18:00 2010
From: b-frederick <@t> northwestern.edu (Bernice Frederick)
Date: Thu May 20 08:18:47 2010
Subject: [Histonet] PPE
In-Reply-To: <4BF42BFA.6030006@yale.edu>
Message-ID: <5BF58ED14E76411B83509FC5FC596FED@lurie.northwestern.edu>

We require gloves for sterile sectioning as we are usually taking sections
for DNA and RNA (and a mask).Otherwise gloves etc are optional. Nothing for
embedding.


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DeRiso
Sent: Wednesday, May 19, 2010 1:21 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] PPE

Does any one use or require gloves when cutting and embedding? How about 
safety goggles when embedding?
Thanks in advance-
Cindy DeRiso
Yale University Pathology

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From Reuel.Cornelia <@t> tsrh.org  Thu May 20 09:17:31 2010
From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia)
Date: Thu May 20 09:17:53 2010
Subject: [Histonet] Large fibrous Bone
Message-ID: <4BF4FE2B020000C500078F1D@mail.TSRH.ORG>

I would like to thank everyone for their contribution on how to remedy our large fibrous bone tissue. Just for your knowledge, this case is a Congenital Pseudoathrosis(CPT) and we have process them both for paraffin and MMA. The paraffin was the choice for our study since we will be doing a lot of IHC studies on this. I was able to cut this hard fibrous tissue by placing them longer(1 hr) on ice and constanly surface decal (30minutes) even if a complete decal was achieve. Thank you histonetters for your unselfish knowledge and experience. 



Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768



From CIngles <@t> uwhealth.org  Thu May 20 09:47:50 2010
From: CIngles <@t> uwhealth.org (Ingles Claire )
Date: Thu May 20 09:47:57 2010
Subject: [Histonet] Sodium borate?
Message-ID: 

Help!
Does anyone know a substitute for sodium borate in the GMS stain? Ours got unknowingly thrown out by our out-date police and he didn't tell anyone, and now we need it ASAP. And we don't have half of the stuff for the Warthin-Starry either. (Derm lab. We do this stuff oh so often, but now of course it is a stat case.) Or if anyone knows a halfway easy stain for, I'm assuming, spirochetes.
Claire
 

From tgenade <@t> gmail.com  Thu May 20 09:51:01 2010
From: tgenade <@t> gmail.com (Tyrone Genade)
Date: Thu May 20 09:51:10 2010
Subject: [Histonet] differentiation of optic tectum layers
Message-ID: 

Hello,

I want to stain sections of optic tectum to show the different
cellular layers. I am planning on using cresyl violet and luxol fast
blue. Anyone have any better ideas? I'm going to try H&E as well
simply because everyone knows it.

Thanks
-- 
Tyrone Genade
http://tgenade.freeshell.org
email: tgenade@gmail.com
tel: +27-84-632-1925 (c)
********************************************************************************
Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.

From contact <@t> excaliburpathology.com  Thu May 20 11:05:24 2010
From: contact <@t> excaliburpathology.com (Paula Pierce)
Date: Thu May 20 11:05:29 2010
Subject: [Histonet] Sodium borate?
In-Reply-To: 
References: 
Message-ID: <21032.12279.qm@web1112.biz.mail.sk1.yahoo.com>

20 Mule Team Borax?in the laundry soap aisle. ;)




________________________________
From: Ingles Claire 
To: histonet@lists.utsouthwestern.edu
Sent: Thu, May 20, 2010 9:47:50 AM
Subject: [Histonet] Sodium borate?

Help!
Does anyone know a substitute for sodium borate in the GMS stain? Ours got unknowingly thrown out by our out-date police and he didn't tell anyone, and now we need it ASAP. And we don't have half of the stuff for the Warthin-Starry either. (Derm lab. We do this stuff oh so often, but now of course it is a stat case.) Or if anyone knows a halfway easy stain for, I'm assuming, spirochetes.
Claire


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From Doug.Showers <@t> propath.com  Thu May 20 12:05:06 2010
From: Doug.Showers <@t> propath.com (Doug Showers)
Date: Thu May 20 12:05:24 2010
Subject: [Histonet] Job opening in Dallas, Texas
Message-ID: <82C7248978CB50469FD6BA68EBBEFE67024961ED@exchange.propathlab.com>

HISTOTECHNOLOGIST

 

ProPath, a high volume, pathology practice, located in Dallas, Texas, has an
immediate opening for a Histotechnologist. 

 

 Responsibilities include embedding tissue specimens, microtomy of
paraffin-embedded tissue, operation of automated stainer and coverslipper,
equipment maintenance and record retention.  

 

The ideal candidate will have a high school diploma or equivalent.  We
prefer, HT, HTL (ASCP) registered or eligible.

 

Benefits include medical, dental, Short and Long Term Disability insurance, a
matched 401K plan and more!  

 

For consideration send resume to:

ProPath, Human Resources

1355 River Bend Drive

Dallas, TX 75247 

FAX: 214/237-1825

Job-Line: 214/237-1775

Email address: jobs@propath.com 

Website: www.propath.com  

 

Don't Follow the Leader!  Join the Leader!

 

EOE

 

 

Doug Showers, MS, HT

Histology Manager

ProPath

1355 River Bend

Dallas, TX 75247

214-237-1680

214-422-3083 Mobile

 

To learn more about ProPath, please visit http://www.ProPathLab.com
 

 

From Lori.Disher <@t> HCAhealthcare.com  Thu May 20 12:53:06 2010
From: Lori.Disher <@t> HCAhealthcare.com (Lori.Disher@HCAhealthcare.com)
Date: Thu May 20 12:53:12 2010
Subject: [Histonet] RE: alternative for bunsen burner
In-Reply-To: <201005191448.o4JEmXuQ022362@NADCLZMSGPMG01A.medcity.net>
References: <201005191448.o4JEmXuQ022362@NADCLZMSGPMG01A.medcity.net>
Message-ID: <778DD853CF606049A37FC2059C8BA07A62AA3D876C@FWDCWPMSGCMS04.hca.corpad.net>

TBS has an electric 3 well forceps warmer catalog #FW-120


Message: 3
Date: Tue, 18 May 2010 10:26:17 -0700
From: Pat Laurie 
Subject: Re: [Histonet] alternative for bunsen burner?
To: Brandi Higgins 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

We use one of the "Bactincinerator" sterilizer that Micro uses.  I believe we got ours through cardinal.

On Tue, May 18, 2010 at 8:30 AM, Brandi Higgins wrote:

> Hello all,
>
> Our hospital is moving our histology department across the hall and 
> have asked us if we can find an alternative to the bunsen burner so 
> they don't have to install a gas line.  The pathologist has told me 
> that there is some machine that can be used instead of a flame to burn 
> the forceps while embedding (which is our main use for the bunsen 
> flame).  This is the only lab I have ever worked at and I don't know 
> what such a machine would be called.  If anyone knows the name, or any 
> other alternative method can you let me know.  Model numbers/companies 
> would be a bonus too!  Thanks so much for your help.
>
> Brandi Higgins, BS, HT(ASCP)
> _______________________________________________
>  Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From laurie.colbert <@t> huntingtonhospital.com  Thu May 20 13:13:28 2010
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Thu May 20 13:13:33 2010
Subject: [Histonet] Mounting Medium for Immunofluorescence
Message-ID: <57BE698966D5C54EAE8612E8941D768308BCB194@EXCHANGE3.huntingtonhospital.com>

We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium.  By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides.  This
mounting medium is described as "Mounting medium for fluorescence, with
propidium iodide."

Will this make a difference when reading the slides??

 

Laurie Colbert

From liz <@t> premierlab.com  Thu May 20 13:48:53 2010
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Thu May 20 13:49:06 2010
Subject: [Histonet] Mounting Medium for Immunofluorescence
In-Reply-To: <57BE698966D5C54EAE8612E8941D768308BCB194@EXCHANGE3.huntingtonhospital.com>
Message-ID: 

I think that the propidium iodide will stain the nuclei or DNA, similar
to DAPI, so this mounting media will counterstain the nuclei and its
visible in the FITC wavelength (488), while if you conterstain with DAPI
it is excited with a different wavelength, therefore it might interfere
with the FITC portion of your stain.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence

We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium.  By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides.  This
mounting medium is described as "Mounting medium for fluorescence, with
propidium iodide."

Will this make a difference when reading the slides??

 

Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From leiker <@t> buffalo.edu  Thu May 20 14:04:17 2010
From: leiker <@t> buffalo.edu (Merced M Leiker)
Date: Thu May 20 14:04:58 2010
Subject: [Histonet] Mounting Medium for Immunofluorescence
In-Reply-To: <57BE698966D5C54EAE8612E8941D768308BCB194@EXCHANGE3.huntingtonhospital.com>
References: <57BE698966D5C54EAE8612E8941D768308BCB194@EXCHANGE3.huntingtonhospital.com>
Message-ID: <518CF2B32C2D4F37AA7BAC99@CDYwxp1931.ad.med.buffalo.edu>

Hi Laurie,

Propidium iodide is a DNA intercalator that fluoresces red (ex 488nm).

Regards,
Merced

--On Thursday, May 20, 2010 11:13 AM -0700 Laurie Colbert 
 wrote:

> We normally coverslip our immunofluorescence slides with a permanent
> aqueous mounting medium.  By mistake, today they were coverslipped with
> a mounting medium that we use for coverslipping our FISH slides.  This
> mounting medium is described as "Mounting medium for fluorescence, with
> propidium iodide."
>
> Will this make a difference when reading the slides??
>
>
>
> Laurie Colbert
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


From LINDA.MARGRAF <@t> childrens.com  Thu May 20 14:04:15 2010
From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF)
Date: Thu May 20 14:06:21 2010
Subject: [Histonet] weekend fixation
Message-ID: <4BF5415F.F783.00DA.0@childrens.com>


 Histonetters:
Here's a message I was asked to post......

Dear Colleagues,
 I  have the following question concerning tissue processing. We do a lot of IHC work on NF fixed tissue. To standardize and minimize the effect of NF fixation, we fixate the tissue always for 24h. This is of course a problem for tissues taken on Friday. In the past, we asked our technicians to come on Saturday to embed the tissues in paraffin. Unfortunately, this is not possible anymore, and that is why I need your advice. What would you suggest ? 1) to leave the tissue in NF until Sunday evening and start processing, or 2) to keep the fixation time (24 hours) and leave the tissue in alcohol 70% until Sunday evening and then start processing.
 Thanks for your advice.
Kind regards,
Wim.
 

Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM



Please consider the environment before printing this e-mail.

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
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strictly prohibited and possibly a violation of federal or state law and regulations. If you
have received this information in error, please notify Children's Medical Center Dallas 
immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical
Center Dallas and its affiliates hereby claim all applicable privileges related to this
information.


From leiker <@t> buffalo.edu  Thu May 20 14:18:33 2010
From: leiker <@t> buffalo.edu (Merced M Leiker)
Date: Thu May 20 14:18:54 2010
Subject: [Histonet] Mounting Medium for Immunofluorescence
In-Reply-To: 
References: 
Message-ID: <884B68E0132713573E61755D@CDYwxp1931.ad.med.buffalo.edu>

Actually if I could make a minor correction to your statement: propidium 
iodide is excited by green light at 488nm but emits in the red portion of 
the spectrum (620nm)...


--On Thursday, May 20, 2010 12:48 PM -0600 Liz Chlipala 
 wrote:

> I think that the propidium iodide will stain the nuclei or DNA, similar
> to DAPI, so this mounting media will counterstain the nuclei and its
> visible in the FITC wavelength (488), while if you conterstain with DAPI
> it is excited with a different wavelength, therefore it might interfere
> with the FITC portion of your stain.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, Colorado 80308
> office (303) 682-3949
> fax (303) 682-9060
> www.premierlab.com
>
>
> Ship to Address:
> 1567 Skyway Drive, Unit E
> Longmont, Colorado 80504
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie
> Colbert
> Sent: Thursday, May 20, 2010 12:13 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Mounting Medium for Immunofluorescence
>
> We normally coverslip our immunofluorescence slides with a permanent
> aqueous mounting medium.  By mistake, today they were coverslipped with
> a mounting medium that we use for coverslipping our FISH slides.  This
> mounting medium is described as "Mounting medium for fluorescence, with
> propidium iodide."
>
> Will this make a difference when reading the slides??
>
>
>
> Laurie Colbert
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


From flnails <@t> texaschildrens.org  Thu May 20 14:29:00 2010
From: flnails <@t> texaschildrens.org (Nails, Felton)
Date: Thu May 20 14:29:14 2010
Subject: [Histonet] PBA for Immunofluorescence
In-Reply-To: 
References: <57BE698966D5C54EAE8612E8941D768308BCB194@EXCHANGE3.huntingtonhospital.com>
	
Message-ID: 

Concerning Immunofluorescence, where are you getting your (PBA) Protein Blocking Agent from? I use to order it from Thermo Shandon but they no longer carry it. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Thursday, May 20, 2010 1:49 PM
To: Laurie Colbert; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Mounting Medium for Immunofluorescence

I think that the propidium iodide will stain the nuclei or DNA, similar to DAPI, so this mounting media will counterstain the nuclei and its visible in the FITC wavelength (488), while if you conterstain with DAPI it is excited with a different wavelength, therefore it might interfere with the FITC portion of your stain.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence

We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium.  By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides.  This mounting medium is described as "Mounting medium for fluorescence, with propidium iodide."

Will this make a difference when reading the slides??

 

Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------------------------------------------------------
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 The information in this e-mail may be confidential and/or
 privileged.  If you are not the intended recipient or an
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From liz <@t> premierlab.com  Thu May 20 14:31:31 2010
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Thu May 20 14:31:39 2010
Subject: [Histonet] RE: PBA for Immunofluorescence
In-Reply-To: 
Message-ID: 

We just use Dako's serum free protein block for IF.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: Nails, Felton [mailto:flnails@texaschildrens.org] 
Sent: Thursday, May 20, 2010 1:29 PM
To: Liz Chlipala; Laurie Colbert; histonet@lists.utsouthwestern.edu
Subject: PBA for Immunofluorescence

Concerning Immunofluorescence, where are you getting your (PBA) Protein
Blocking Agent from? I use to order it from Thermo Shandon but they no
longer carry it. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Thursday, May 20, 2010 1:49 PM
To: Laurie Colbert; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Mounting Medium for Immunofluorescence

I think that the propidium iodide will stain the nuclei or DNA, similar
to DAPI, so this mounting media will counterstain the nuclei and its
visible in the FITC wavelength (488), while if you conterstain with DAPI
it is excited with a different wavelength, therefore it might interfere
with the FITC portion of your stain.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC
PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303)
682-9060 www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence

We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium.  By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides.  This
mounting medium is described as "Mounting medium for fluorescence, with
propidium iodide."

Will this make a difference when reading the slides??

 

Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------------------------------------------------
------
CONFIDENTIALITY NOTICE:
 The information in this e-mail may be confidential and/or
 privileged.  If you are not the intended recipient or an
 authorized representative of the intended recipient, you
 are hereby notified that any review, dissemination, or
 copying of this e-mail and its attachments, if any, or
 the information contained herein is prohibited.  If you
 have received this e-mail in error, please immediately
 notify the sender by return e-mail and delete this e-mail
 from your computer system.  Thank you.
========================================================================
======


From Debra.Ortiz <@t> uchospitals.edu  Thu May 20 14:41:09 2010
From: Debra.Ortiz <@t> uchospitals.edu (Debra.Ortiz@uchospitals.edu)
Date: Thu May 20 14:41:32 2010
Subject: [Histonet] possible AFB contaminant
Message-ID: <5392DB699B157E4C8E65B3779634BD7D011AD985@uchmbx04-hpk03s.UCHAD.uchospitals.edu>

Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are "false positives" on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths.
Any advice or comments would be greatly appreciated.
 
Thanks Debi

********************************************************************************
This e-mail is intended only for the use of the individual or entity to which
it is addressed and may contain information that is privileged and confidential.
If the reader of this e-mail message is not the intended recipient, you are 
hereby notified that any dissemination, distribution or copying of this
communication is prohibited. If you have received this e-mail in error, please 
notify the sender and destroy all copies of the transmittal. 

Thank you
University of Chicago Medical Center 
********************************************************************************

From baderbo <@t> gmail.com  Thu May 20 14:52:47 2010
From: baderbo <@t> gmail.com (Bader Siddiki)
Date: Thu May 20 14:52:56 2010
Subject: [Histonet] Mounting medium for IF
Message-ID: 

Hello Laurie
Pi gives red color in IF, it stains DNA and RNA

PI excites at 535nmand emits at 615nm, producing a *red* fluorescence.

Therefore if you are using any chromophore at these wave lengths which emits
red color you will have problems.

By the way we also have several mounting mediums for IHC and IF, if you
would like to try, please let us know.

Bader
**



-- 
If any Q's please feel free to contact us
Have a nice day/weekend
Mit freundlichen Gr??en / With Kind Regards /
avec l'aimable ce qui concerne
Met vriendelijke groeten
??????
Bader
Bader B Siddiki, PhD
Executive director,
Research and development
ImmunoBioScience corp.
Phone: + 425 367 4601
Phone: + 425 514 3761
Fax: + 425 367 4817
cell (mobile) phone: + 425 314 0199
e-mail address: baderbo@gmail.com
Web site: www.immunobioscience.com
Marketing: phone: + 650 343 IBSC (4272)
               E-mail: anitaIBSC@aol.com
From liz <@t> premierlab.com  Thu May 20 14:54:25 2010
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Thu May 20 14:54:37 2010
Subject: [Histonet] weekend fixation
In-Reply-To: <4BF5415F.F783.00DA.0@childrens.com>
Message-ID: 

I was talking to Peggy Wenk over the weekend at the MSH meeting and they
had a paper that was published regarding fixation and ER/PR staining
sensitivity etc.  The biggest problem that they reported is
underfixation is much worse than over fixation.  I think a minimum of 10
hours of fixation demonstrated good results and that intensity of
staining started to decrease but not by much at 48 hours.  I would be
more concerned over underfixation than overfixation.  Also the new ER/PR
guidelines state its acceptable to have samples in fixative for 72
hours.  Maybe Peggy can post the link to this paper.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA
MARGRAF
Sent: Thursday, May 20, 2010 1:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] weekend fixation


 Histonetters:
Here's a message I was asked to post......

Dear Colleagues,
 I  have the following question concerning tissue processing. We do a
lot of IHC work on NF fixed tissue. To standardize and minimize the
effect of NF fixation, we fixate the tissue always for 24h. This is of
course a problem for tissues taken on Friday. In the past, we asked our
technicians to come on Saturday to embed the tissues in paraffin.
Unfortunately, this is not possible anymore, and that is why I need your
advice. What would you suggest ? 1) to leave the tissue in NF until
Sunday evening and start processing, or 2) to keep the fixation time (24
hours) and leave the tissue in alcohol 70% until Sunday evening and then
start processing.
 Thanks for your advice.
Kind regards,
Wim.
 

Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM



Please consider the environment before printing this e-mail.

This e-mail, facsimile, or letter and any files or attachments
transmitted with it contains
information that is confidential and privileged. This information is
intended only for the
use of the individual(s) and entity(ies) to whom it is addressed. If you
are the intended
recipient, further disclosures are prohibited without proper
authorization. If you are not
the intended recipient, any disclosure, copying, printing, or use of
this information is
strictly prohibited and possibly a violation of federal or state law and
regulations. If you
have received this information in error, please notify Children's
Medical Center Dallas 
immediately at 214-456-4444 or via e-mail at privacy@childrens.com.
Children's Medical
Center Dallas and its affiliates hereby claim all applicable privileges
related to this
information.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From liz <@t> premierlab.com  Thu May 20 15:00:05 2010
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Thu May 20 15:00:16 2010
Subject: [Histonet] weekend fixation
In-Reply-To: <4BF5415F.F783.00DA.0@childrens.com>
Message-ID: 

I found the reference

Goldstein NS, Ferkowicz M, Odish E, et al: Minimum formalin fixation
time for consistent estrogen receptor immunohistochemical staining of
invasive breast carcinoma Am J Clin Pathol 120:86-92, 2003

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA
MARGRAF
Sent: Thursday, May 20, 2010 1:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] weekend fixation


 Histonetters:
Here's a message I was asked to post......

Dear Colleagues,
 I  have the following question concerning tissue processing. We do a
lot of IHC work on NF fixed tissue. To standardize and minimize the
effect of NF fixation, we fixate the tissue always for 24h. This is of
course a problem for tissues taken on Friday. In the past, we asked our
technicians to come on Saturday to embed the tissues in paraffin.
Unfortunately, this is not possible anymore, and that is why I need your
advice. What would you suggest ? 1) to leave the tissue in NF until
Sunday evening and start processing, or 2) to keep the fixation time (24
hours) and leave the tissue in alcohol 70% until Sunday evening and then
start processing.
 Thanks for your advice.
Kind regards,
Wim.
 

Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM



Please consider the environment before printing this e-mail.

This e-mail, facsimile, or letter and any files or attachments
transmitted with it contains
information that is confidential and privileged. This information is
intended only for the
use of the individual(s) and entity(ies) to whom it is addressed. If you
are the intended
recipient, further disclosures are prohibited without proper
authorization. If you are not
the intended recipient, any disclosure, copying, printing, or use of
this information is
strictly prohibited and possibly a violation of federal or state law and
regulations. If you
have received this information in error, please notify Children's
Medical Center Dallas 
immediately at 214-456-4444 or via e-mail at privacy@childrens.com.
Children's Medical
Center Dallas and its affiliates hereby claim all applicable privileges
related to this
information.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From sgoebel <@t> xbiotech.com  Thu May 20 15:08:17 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Thu May 20 15:08:36 2010
Subject: [Histonet] weekend fixation
Message-ID: <20100520130817.9e2d9aa830e8449a2412eb1e4f2f067e.23e654c58e.wbe@email04.secureserver.net>


   From  what I have seen and heard you can have fixation times up to   hours and still be ok?

   Sarah Goebel, B.A., HT (ASCP)

   <
   Histo   
   XBiotech USA Inc.

   8201 East Rivers
   Austin, Texas  78744
   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] weekend fixation
   From: "LINDA MARGRAF" 
   Date: Thu, May 20, 2010 12:04 pm
   To: 
   Histonetters:
   Here's a message I was asked to post......
   Dear Colleagues,
   I  have  the  following question concerning tissue processing. We do a
   lot  o   effect  of  NF   of  course  a  problem   asked  our  technicians  to  come   paraffin.  Unfortunately,  this is not p   why  I  need  your  advice.  What would you suggest   tissue  in  NF  until  Sunday evening and start processing,    keep  the  fixation  time (24 hours) and leave the tissue in alcohol    70% until Sunday evening and then start processing.
   Thanks for your advice.
   Kind regards,
   Wim.
   Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
   Cell biology and Histology
   Department of Morphology - Faculty of Veterinary Medicine
   Ghent University
   Salisburylaan 133, B-9820 Merelbeke, BELGIUM
   Please consider the environment before printing this e-mail.
   This  e-mail,  facsimile,  or  letter  and  any  files  or attachments
   transmitted    information  that  is confidential and privileged. This information is
   intend   use  of  the individual(s) and entity(ies) to whom it is addressed. If
   you ar   recipient,   further   disclosures   are   prohibited  without  proper
   authorization.   the  intended  recipient, any disclosure, copying, printing, or use of
   this i   strictly  prohibited  and possibly a violation of federal or state law
   and re   have  received  this  information  in  error, please notify Children's
   Medical C   immediately  at  214-456-4444  or via e-mail at privacy@childrens.com.
   Childre   Center   Dallas   and  its  affiliates  hereby  claim  all  applicable
   privileges rel   information.
   _______________________________________________
   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
References

   1. 3D"http://lists.utsouthwestern.edu/mailman/listin
From shive003 <@t> umn.edu  Thu May 20 15:22:48 2010
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Thu May 20 15:23:00 2010
Subject: [Histonet] possible AFB contaminant
References: <5392DB699B157E4C8E65B3779634BD7D011AD985@uchmbx04-hpk03s.UCHAD.uchospitals.edu>
Message-ID: <6AA2659851A64D6B9697A8697E4AAD20@auxs.umn.edu>

How often are you decontaminating your NeXes machines (running 
decontaminating reagents through the tubing)?

Jan Shivers
UMN VetDiagLab

----- Original Message ----- 
From: 
To: 
Sent: Thursday, May 20, 2010 2:41 PM
Subject: [Histonet] possible AFB contaminant


Please help, we have had an increase in demands for repeats on our AFB 
stain. Our pathologists have been noticing what they believe are "false 
positives" on our slides. We perform our AFBs on the Ventana Nexus and some 
are done by hand. Because of this problem, we have been doing side by side 
comparisons using the Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits, tested 
our DI water sytem and have different techs cutting using different 
waterbaths.
Any advice or comments would be greatly appreciated.

Thanks Debi

********************************************************************************
This e-mail is intended only for the use of the individual or entity to 
which
it is addressed and may contain information that is privileged and 
confidential.
If the reader of this e-mail message is not the intended recipient, you are
hereby notified that any dissemination, distribution or copying of this
communication is prohibited. If you have received this e-mail in error, 
please
notify the sender and destroy all copies of the transmittal.

Thank you
University of Chicago Medical Center
********************************************************************************

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From alexandra.meinl <@t> gmail.com  Thu May 20 16:03:21 2010
From: alexandra.meinl <@t> gmail.com (Alexandra Meinl)
Date: Thu May 20 16:03:26 2010
Subject: [Histonet] weekend fixation
In-Reply-To: <4BF5415F.F783.00DA.0@childrens.com>
References: <4BF5415F.F783.00DA.0@childrens.com>
Message-ID: 

We fix all specimens in NBF for 24h, wash them in tap water and store them
in alcohol 70% at 4?C until further processing. That works perfectly well
for us, and we never noticed any loss of staining.
But I also knew that some colleagues prefer to run a weekend program on
their Tissue Tek without delay so that the specimens stay infiltrated with
paraffin until Monday morning.

Alexandra



************************************************
Dr. Alexandra Meinl
Ludwig Boltzmann Institute
for Experimental and Clinical Traumatology
Austrian Cluster for Tissue Regeneration
Histology
Donaueschingenstrasse 13
1200 Vienna - Austria
From marktarango <@t> gmail.com  Thu May 20 16:14:41 2010
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Thu May 20 16:14:46 2010
Subject: [Histonet] weekend fixation
In-Reply-To: 
References: <4BF5415F.F783.00DA.0@childrens.com>
	
Message-ID: 

Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if
you do HER2 the maximum is still 48 hours.  I'm assuming you want HER2 as
well, so your best option would probably be to hold the tissues in 70%
alcochol on the processer until Sunday night.

Mark Tarango
On Thu, May 20, 2010 at 12:54 PM, Liz Chlipala  wrote:

> I was talking to Peggy Wenk over the weekend at the MSH meeting and they
> had a paper that was published regarding fixation and ER/PR staining
> sensitivity etc.  The biggest problem that they reported is
> underfixation is much worse than over fixation.  I think a minimum of 10
> hours of fixation demonstrated good results and that intensity of
> staining started to decrease but not by much at 48 hours.  I would be
> more concerned over underfixation than overfixation.  Also the new ER/PR
> guidelines state its acceptable to have samples in fixative for 72
> hours.  Maybe Peggy can post the link to this paper.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, Colorado 80308
> office (303) 682-3949
> fax (303) 682-9060
> www.premierlab.com
>
>
> Ship to Address:
> 1567 Skyway Drive, Unit E
> Longmont, Colorado 80504
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA
> MARGRAF
> Sent: Thursday, May 20, 2010 1:04 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] weekend fixation
>
>
>   Histonetters:
> Here's a message I was asked to post......
>
> Dear Colleagues,
>  I  have the following question concerning tissue processing. We do a
> lot of IHC work on NF fixed tissue. To standardize and minimize the
> effect of NF fixation, we fixate the tissue always for 24h. This is of
> course a problem for tissues taken on Friday. In the past, we asked our
> technicians to come on Saturday to embed the tissues in paraffin.
> Unfortunately, this is not possible anymore, and that is why I need your
> advice. What would you suggest ? 1) to leave the tissue in NF until
> Sunday evening and start processing, or 2) to keep the fixation time (24
> hours) and leave the tissue in alcohol 70% until Sunday evening and then
> start processing.
>  Thanks for your advice.
> Kind regards,
> Wim.
>
>
> Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
> Cell biology and Histology
> Department of Morphology - Faculty of Veterinary Medicine
> Ghent University
> Salisburylaan 133, B-9820 Merelbeke, BELGIUM
>
>
>
> Please consider the environment before printing this e-mail.
>
> This e-mail, facsimile, or letter and any files or attachments
> transmitted with it contains
> information that is confidential and privileged. This information is
> intended only for the
> use of the individual(s) and entity(ies) to whom it is addressed. If you
> are the intended
> recipient, further disclosures are prohibited without proper
> authorization. If you are not
> the intended recipient, any disclosure, copying, printing, or use of
> this information is
> strictly prohibited and possibly a violation of federal or state law and
> regulations. If you
> have received this information in error, please notify Children's
> Medical Center Dallas
> immediately at 214-456-4444 or via e-mail at privacy@childrens.com.
> Children's Medical
> Center Dallas and its affiliates hereby claim all applicable privileges
> related to this
> information.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From marktarango <@t> gmail.com  Thu May 20 16:17:10 2010
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Thu May 20 16:17:18 2010
Subject: [Histonet] weekend fixation
In-Reply-To: 
References: <4BF5415F.F783.00DA.0@childrens.com>
	
	
Message-ID: 

I just realized the question came from Belgium.  I have no idea how they do
things there.

Mark

On Thu, May 20, 2010 at 2:14 PM, Mark Tarango  wrote:

> Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if
> you do HER2 the maximum is still 48 hours.  I'm assuming you want HER2 as
> well, so your best option would probably be to hold the tissues in 70%
> alcochol on the processer until Sunday night.
>
> Mark Tarango
>   On Thu, May 20, 2010 at 12:54 PM, Liz Chlipala wrote:
>
>> I was talking to Peggy Wenk over the weekend at the MSH meeting and they
>> had a paper that was published regarding fixation and ER/PR staining
>> sensitivity etc.  The biggest problem that they reported is
>> underfixation is much worse than over fixation.  I think a minimum of 10
>> hours of fixation demonstrated good results and that intensity of
>> staining started to decrease but not by much at 48 hours.  I would be
>> more concerned over underfixation than overfixation.  Also the new ER/PR
>> guidelines state its acceptable to have samples in fixative for 72
>> hours.  Maybe Peggy can post the link to this paper.
>>
>> Liz
>>
>> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>> Manager
>> Premier Laboratory, LLC
>> PO Box 18592
>> Boulder, Colorado 80308
>> office (303) 682-3949
>> fax (303) 682-9060
>> www.premierlab.com
>>
>>
>> Ship to Address:
>> 1567 Skyway Drive, Unit E
>> Longmont, Colorado 80504
>>
>> -----Original Message-----
>> From: histonet-bounces@lists.utsouthwestern.edu
>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA
>> MARGRAF
>> Sent: Thursday, May 20, 2010 1:04 PM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] weekend fixation
>>
>>
>>   Histonetters:
>> Here's a message I was asked to post......
>>
>> Dear Colleagues,
>>  I  have the following question concerning tissue processing. We do a
>> lot of IHC work on NF fixed tissue. To standardize and minimize the
>> effect of NF fixation, we fixate the tissue always for 24h. This is of
>> course a problem for tissues taken on Friday. In the past, we asked our
>> technicians to come on Saturday to embed the tissues in paraffin.
>> Unfortunately, this is not possible anymore, and that is why I need your
>> advice. What would you suggest ? 1) to leave the tissue in NF until
>> Sunday evening and start processing, or 2) to keep the fixation time (24
>> hours) and leave the tissue in alcohol 70% until Sunday evening and then
>> start processing.
>>  Thanks for your advice.
>> Kind regards,
>> Wim.
>>
>>
>> Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
>> Cell biology and Histology
>> Department of Morphology - Faculty of Veterinary Medicine
>> Ghent University
>> Salisburylaan 133, B-9820 Merelbeke, BELGIUM
>>
>>
>>
>> Please consider the environment before printing this e-mail.
>>
>> This e-mail, facsimile, or letter and any files or attachments
>> transmitted with it contains
>> information that is confidential and privileged. This information is
>> intended only for the
>> use of the individual(s) and entity(ies) to whom it is addressed. If you
>> are the intended
>> recipient, further disclosures are prohibited without proper
>> authorization. If you are not
>> the intended recipient, any disclosure, copying, printing, or use of
>> this information is
>> strictly prohibited and possibly a violation of federal or state law and
>> regulations. If you
>> have received this information in error, please notify Children's
>> Medical Center Dallas
>> immediately at 214-456-4444 or via e-mail at privacy@childrens.com.
>> Children's Medical
>> Center Dallas and its affiliates hereby claim all applicable privileges
>> related to this
>> information.
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
From mpence <@t> grhs.net  Thu May 20 16:28:01 2010
From: mpence <@t> grhs.net (Mike Pence)
Date: Thu May 20 16:28:13 2010
Subject: [Histonet] weekend fixation
In-Reply-To: 
Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3E21@is-e2k3.grhs.net>

The problem with all this is that the ER/PR fixation times and the her2
fixation times do not match 72 hrs vs 48 hrs. Many times the same
specimen that receives ER/PR also gets her2. This is where things are
missed up. Why would they ever change one and not the other?!@#$%^&*

Mike

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Thursday, May 20, 2010 2:54 PM
To: LINDA MARGRAF; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] weekend fixation


I was talking to Peggy Wenk over the weekend at the MSH meeting and they
had a paper that was published regarding fixation and ER/PR staining
sensitivity etc.  The biggest problem that they reported is
underfixation is much worse than over fixation.  I think a minimum of 10
hours of fixation demonstrated good results and that intensity of
staining started to decrease but not by much at 48 hours.  I would be
more concerned over underfixation than overfixation.  Also the new ER/PR
guidelines state its acceptable to have samples in fixative for 72
hours.  Maybe Peggy can post the link to this paper.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA
MARGRAF
Sent: Thursday, May 20, 2010 1:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] weekend fixation


 Histonetters:
Here's a message I was asked to post......

Dear Colleagues,
 I  have the following question concerning tissue processing. We do a
lot of IHC work on NF fixed tissue. To standardize and minimize the
effect of NF fixation, we fixate the tissue always for 24h. This is of
course a problem for tissues taken on Friday. In the past, we asked our
technicians to come on Saturday to embed the tissues in paraffin.
Unfortunately, this is not possible anymore, and that is why I need your
advice. What would you suggest ? 1) to leave the tissue in NF until
Sunday evening and start processing, or 2) to keep the fixation time (24
hours) and leave the tissue in alcohol 70% until Sunday evening and then
start processing.  Thanks for your advice. Kind regards, Wim.
 

Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM



Please consider the environment before printing this e-mail.

This e-mail, facsimile, or letter and any files or attachments
transmitted with it contains information that is confidential and
privileged. This information is intended only for the use of the
individual(s) and entity(ies) to whom it is addressed. If you are the
intended recipient, further disclosures are prohibited without proper
authorization. If you are not the intended recipient, any disclosure,
copying, printing, or use of this information is strictly prohibited and
possibly a violation of federal or state law and regulations. If you
have received this information in error, please notify Children's
Medical Center Dallas 
immediately at 214-456-4444 or via e-mail at privacy@childrens.com.
Children's Medical Center Dallas and its affiliates hereby claim all
applicable privileges related to this information.


_______________________________________________
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From mpence <@t> grhs.net  Thu May 20 16:28:51 2010
From: mpence <@t> grhs.net (Mike Pence)
Date: Thu May 20 16:28:58 2010
Subject: [Histonet] weekend fixation
In-Reply-To: <20100520130817.9e2d9aa830e8449a2412eb1e4f2f067e.23e654c58e.wbe@email04.secureserver.net>
Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3E22@is-e2k3.grhs.net>

Yeah. Good luck with that one. The minimum clearly states 6 hrs.

Mike

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
sgoebel@xbiotech.com
Sent: Thursday, May 20, 2010 3:08 PM
To: LINDA MARGRAF
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] weekend fixation



   From  what I have seen and heard you can have fixation times up to=2
   hours and still be ok?

   Sarah Goebel, B.A., HT (ASCP)

   <=iv>

   Histo=echnician
   
   XBiotech USA Inc.

   8201 East Rivers=de Dr. Bldg 4 Suite 100

   Austin, Texas  78744
   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] weekend fixation
   From: "LINDA MARGRAF" 
   Date: Thu, May 20, 2010 12:04 pm
   To: 
   Histonetters:
   Here's a message I was asked to post......
   Dear Colleagues,
   I  have  the  following question concerning tissue processing. We do
a
   lot  o= IHC work on NF fixed tissue. To standardize and minimize the
   effect  of  NF=ixation, we fixate the tissue always for 24h. This is
   of  course  a  problem=for  tissues taken on Friday. In the past, we
   asked  our  technicians  to  come=n Saturday to embed the tissues in
   paraffin.  Unfortunately,  this is not p=ssible anymore, and that is
   why  I  need  your  advice.  What would you suggest= 1) to leave the
   tissue  in  NF  until  Sunday evening and start processing, =r 2) to
   keep  the  fixation  time (24 hours) and leave the tissue in alcohol
70% until Sunday evening and then start processing.
   Thanks for your advice.
   Kind regards,
   Wim.
   Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
   Cell biology and Histology
   Department of Morphology - Faculty of Veterinary Medicine
   Ghent University
   Salisburylaan 133, B-9820 Merelbeke, BELGIUM
   Please consider the environment before printing this e-mail.
   This  e-mail,  facsimile,  or  letter  and  any  files  or
attachments
   transmitted =ith it contains
   information  that  is confidential and privileged. This information
is
   intend=d only for the
   use  of  the individual(s) and entity(ies) to whom it is addressed.
If
   you ar= the intended
   recipient,   further   disclosures   are   prohibited  without
proper
   authorization.=f you are not
   the  intended  recipient, any disclosure, copying, printing, or use
of
   this i=formation is
   strictly  prohibited  and possibly a violation of federal or state
law
   and re=ulations. If you
   have  received  this  information  in  error, please notify
Children's
   Medical C=nter Dallas
   immediately  at  214-456-4444  or via e-mail at
privacy@childrens.com.
   Childre='s Medical
   Center   Dallas   and  its  affiliates  hereby  claim  all
applicable
   privileges rel=ted to this
   information.
   _______________________________________________
   Histonet mailing list
   Histonet@lists.utsouthwestern.edu
   [1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<=r>

References

   1.
3D"http://lists.utsouthwestern.edu/mailman/listin_______________________
________________________
Histonet mailing list
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From mpence <@t> grhs.net  Thu May 20 16:29:57 2010
From: mpence <@t> grhs.net (Mike Pence)
Date: Thu May 20 16:30:09 2010
Subject: [Histonet] possible AFB contaminant
In-Reply-To: <6AA2659851A64D6B9697A8697E4AAD20@auxs.umn.edu>
Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3E23@is-e2k3.grhs.net>

We decontame once a month.

Mike

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan
Shivers
Sent: Thursday, May 20, 2010 3:23 PM
To: Debra.Ortiz@uchospitals.edu; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] possible AFB contaminant


How often are you decontaminating your NeXes machines (running 
decontaminating reagents through the tubing)?

Jan Shivers
UMN VetDiagLab

----- Original Message ----- 
From: 
To: 
Sent: Thursday, May 20, 2010 2:41 PM
Subject: [Histonet] possible AFB contaminant


Please help, we have had an increase in demands for repeats on our AFB 
stain. Our pathologists have been noticing what they believe are "false 
positives" on our slides. We perform our AFBs on the Ventana Nexus and
some 
are done by hand. Because of this problem, we have been doing side by
side 
comparisons using the Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits,
tested 
our DI water sytem and have different techs cutting using different 
waterbaths.
Any advice or comments would be greatly appreciated.

Thanks Debi

************************************************************************
********
This e-mail is intended only for the use of the individual or entity to 
which
it is addressed and may contain information that is privileged and 
confidential.
If the reader of this e-mail message is not the intended recipient, you
are hereby notified that any dissemination, distribution or copying of
this communication is prohibited. If you have received this e-mail in
error, 
please
notify the sender and destroy all copies of the transmittal.

Thank you
University of Chicago Medical Center
************************************************************************
********

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From renafail <@t> bellsouth.net  Thu May 20 17:10:01 2010
From: renafail <@t> bellsouth.net (Rena Fail)
Date: Thu May 20 17:10:14 2010
Subject: [Histonet] possible AFB contaminant
In-Reply-To: <5392DB699B157E4C8E65B3779634BD7D011AD985@uchmbx04-hpk03s.UCHAD.uchospitals.edu>
References: <5392DB699B157E4C8E65B3779634BD7D011AD985@uchmbx04-hpk03s.UCHAD.uchospitals.edu>
Message-ID: <644130.6220.qm@web180309.mail.gq1.yahoo.com>

Are you using tubing on your dispensers for your DI water? They can be a source of contaminant. You can also have cross contaminantion from your control slide during dehydration and hydration as well as during staining.Do not use the same set up for contrpl and pt slide. Lay slides flat for hand staining
Rena Fail



----- Original Message ----
From: "Debra.Ortiz@uchospitals.edu" 
To: histonet@lists.utsouthwestern.edu
Sent: Thu, May 20, 2010 3:41:09 PM
Subject: [Histonet] possible AFB contaminant

Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are "false positives" on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths.
Any advice or comments would be greatly appreciated.

Thanks Debi

********************************************************************************
This e-mail is intended only for the use of the individual or entity to which
it is addressed and may contain information that is privileged and confidential.
If the reader of this e-mail message is not the intended recipient, you are 
hereby notified that any dissemination, distribution or copying of this
communication is prohibited. If you have received this e-mail in error, please 
notify the sender and destroy all copies of the transmittal. 

Thank you
University of Chicago Medical Center 
********************************************************************************

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From marktarango <@t> gmail.com  Thu May 20 17:55:25 2010
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Thu May 20 17:55:30 2010
Subject: [Histonet] weekend fixation
In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3E21@is-e2k3.grhs.net>
References: 
	<661949901A768E4F9CC16D8AF8F2838C017A3E21@is-e2k3.grhs.net>
Message-ID: 

I heard on a teleconference yesterday that they're going to be changing both
to 72 hours max time fixation, but until its the published guidlines 48
hours still stands for her2neu.

Mark

On Thu, May 20, 2010 at 2:28 PM, Mike Pence  wrote:

> The problem with all this is that the ER/PR fixation times and the her2
> fixation times do not match 72 hrs vs 48 hrs. Many times the same
> specimen that receives ER/PR also gets her2. This is where things are
> missed up. Why would they ever change one and not the other?!@#$%^&*
>
> Mike
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
>  [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz
> Chlipala
> Sent: Thursday, May 20, 2010 2:54 PM
> To: LINDA MARGRAF; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] weekend fixation
>
>
> I was talking to Peggy Wenk over the weekend at the MSH meeting and they
> had a paper that was published regarding fixation and ER/PR staining
> sensitivity etc.  The biggest problem that they reported is
> underfixation is much worse than over fixation.  I think a minimum of 10
> hours of fixation demonstrated good results and that intensity of
> staining started to decrease but not by much at 48 hours.  I would be
> more concerned over underfixation than overfixation.  Also the new ER/PR
> guidelines state its acceptable to have samples in fixative for 72
> hours.  Maybe Peggy can post the link to this paper.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, Colorado 80308
> office (303) 682-3949
> fax (303) 682-9060
> www.premierlab.com
>
>
> Ship to Address:
> 1567 Skyway Drive, Unit E
> Longmont, Colorado 80504
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA
> MARGRAF
> Sent: Thursday, May 20, 2010 1:04 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] weekend fixation
>
>
>  Histonetters:
> Here's a message I was asked to post......
>
> Dear Colleagues,
>  I  have the following question concerning tissue processing. We do a
> lot of IHC work on NF fixed tissue. To standardize and minimize the
> effect of NF fixation, we fixate the tissue always for 24h. This is of
> course a problem for tissues taken on Friday. In the past, we asked our
> technicians to come on Saturday to embed the tissues in paraffin.
> Unfortunately, this is not possible anymore, and that is why I need your
> advice. What would you suggest ? 1) to leave the tissue in NF until
> Sunday evening and start processing, or 2) to keep the fixation time (24
> hours) and leave the tissue in alcohol 70% until Sunday evening and then
> start processing.  Thanks for your advice. Kind regards, Wim.
>
>
> Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
> Cell biology and Histology
> Department of Morphology - Faculty of Veterinary Medicine
> Ghent University
> Salisburylaan 133, B-9820 Merelbeke, BELGIUM
>
>
>
> Please consider the environment before printing this e-mail.
>
> This e-mail, facsimile, or letter and any files or attachments
> transmitted with it contains information that is confidential and
> privileged. This information is intended only for the use of the
> individual(s) and entity(ies) to whom it is addressed. If you are the
> intended recipient, further disclosures are prohibited without proper
> authorization. If you are not the intended recipient, any disclosure,
> copying, printing, or use of this information is strictly prohibited and
> possibly a violation of federal or state law and regulations. If you
> have received this information in error, please notify Children's
> Medical Center Dallas
> immediately at 214-456-4444 or via e-mail at privacy@childrens.com.
> Children's Medical Center Dallas and its affiliates hereby claim all
> applicable privileges related to this information.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From kc <@t> ka-recruiting.com  Thu May 20 18:08:00 2010
From: kc <@t> ka-recruiting.com (K.C. Carpenter)
Date: Thu May 20 18:08:06 2010
Subject: [Histonet] Histology Career Opportunities
Message-ID: <1665843700.1274396879806.JavaMail.cfservice@sl4app2>





Hi Histonet Subscribers, 



 


Are you interested in hearing about new job opportunities? I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have established relationships with clients at leading hospitals and labs across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. 


 


Below is a list of some of the Histology opportunities we are currently working on.



 




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*Atlanta, GA - Atlanta - Pathology Coordinator (HT or CT)



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*New York, NY - Histology Supervisor 


*Upstate NY (Capital District)  - Histotechnologist 1st shift


*Las Vegas, NV - Histotech 3rd shift


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*Palm Springs, CA - Histotech - 1st shift


*Los Angeles, CA -  Histotech


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*Southern NH - HTL and HT








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If you're interested in learning more about any of these opportunities then please email me a resume and let me know how best to get in touch with you.  
If none of these are a fit please let me know what you'd be interested in and where you're looking so I can tailor a search for you.  With the New Year upon us many of our clients have fresh hiring budgets and will be looking to add people over the next several months. We work on positions at all levels and cover the entire US. To view some additional opportunities please visit our website at www.ka-recruiting.com
.  






Sincerely, 












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K.A. Recruiting, Inc.


10 Post Office Square, 8th Floor South


Boston, MA 02109


P: (617) 692-2949


F: (617) 507-8009



kc@ka-recruiting.com



www.ka-recruiting.com
  








From Kevin_Kurtz <@t> ssmhc.com  Thu May 20 20:31:16 2010
From: Kevin_Kurtz <@t> ssmhc.com (Kevin_Kurtz@ssmhc.com)
Date: Thu May 20 20:31:24 2010
Subject: [Histonet] Re: weekend fixation
In-Reply-To: 
References: 
Message-ID: 


   The  CAP has the following to say about this issue (in the FAQ section
   on HE   "The  CAP  Immu   transferring  formalin-fi   no  data to support the validity   in   this   manner,   and   there   is   a  signif   
incompletely   fixed   tissues  will  be  placed  in  alcohol     prematurely  and 
tissue fixation will be completed in alcohol.
   This     prolonged  formal   testing  specimens  that have h   (or    xylene    substitute).    Finally,    it   
unacceptable  to  leave  fully  processed  tissues  in  heate   paraffin for 
prolonged periods."
   

Confident   any
attachments,  is for the    and
may   contain   confidential  and  pr   Any
unauthorized  review,  use,  disclosure  or  distr   prohibited.
If  you  are  not  the  intended recipient, please co   ntact  the  sender  by
reply  email  and  destroy  all copies of the
   original me
From jshea121 <@t> roadrunner.com  Thu May 20 21:05:26 2010
From: jshea121 <@t> roadrunner.com (Shea's)
Date: Thu May 20 21:05:27 2010
Subject: [Histonet] RE: weekend fixation 
Message-ID: <77C9722062474EB285C57B7404E292F2@JoannePC>

We discussed this problem of weekend fixation of breast tissue (about 54 hrs in 10% NBF) with our Reference Lab and they reassured us that ER/PR IHC wouldn't be a problem. However, when ordering Her2, select FISH method instead of IHC. (Otherwise, if it is fixed for less than 48 hrs we would order Her2 by IHC, then reflex 2+ to FISH). Any thoughts about this?
From rcartun <@t> harthosp.org  Thu May 20 22:07:29 2010
From: rcartun <@t> harthosp.org (Richard Cartun)
Date: Thu May 20 22:07:45 2010
Subject: [Histonet] weekend fixation
Message-ID: <4BF5C0B602000077000171E7@gwmail4.harthosp.org>

Dear Colleagues:

Please keep in mind that the ASCO/CAP guidelines are "recommendations", not mandates. You can experiment, validate, and establish your own formalin-fixation protocol for breast specimens. At Hartford Hospital I have established a minimum of 4.5 hours for needle core specimens. This includes 0.5 hour of pre-processor fixation and 4 hours of fixation on our tissue processors. We do use the 6 hour minimum for all excisional specimens. WE HAVE NO MAXIMUM FIXATION TIME. I can show you absolutely beautiful HER2 immunoreactivity and gene amplification on breast cancer tissues that have been fixed for 1 week, 1 month, 1 year, and longer. If you don't agree with the guidelines, work with your pathologist(s) and establish your own fixation policy. And, please remember, fixation time is only one piece to the puzzle. In my experience, minimizing ischemic time, making sure that the tissue does not dry out prior to fixation, and submitting thin (2 mm) sections are probably more important than fixation time.

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimens
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax
>>> "Mike Pence" 05/20/10 6:10 PM >>> 
Yeah. Good luck with that one. The minimum clearly states 6 hrs. 

Mike 

-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of 
sgoebel@xbiotech.com 
Sent: Thursday, May 20, 2010 3:08 PM 
To: LINDA MARGRAF 
Cc: histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] weekend fixation 



>From what I have seen and heard you can have fixation times up to=2 
hours and still be ok? 

Sarah Goebel, B.A., HT (ASCP) 

<=iv> 

Histo=echnician 

XBiotech USA Inc. 

8201 East Rivers=de Dr. Bldg 4 Suite 100 

Austin, Texas 78744 
(512)386-5107 

-------- Original Message -------- 
Subject: [Histonet] weekend fixation 
From: "LINDA MARGRAF" 
Date: Thu, May 20, 2010 12:04 pm 
To: 
Histonetters: 
Here's a message I was asked to post...... 
Dear Colleagues, 
I have the following question concerning tissue processing. We do 
a 
lot o= IHC work on NF fixed tissue. To standardize and minimize the 
effect of NF=ixation, we fixate the tissue always for 24h. This is 
of course a problem=for tissues taken on Friday. In the past, we 
asked our technicians to come=n Saturday to embed the tissues in 
paraffin. Unfortunately, this is not p=ssible anymore, and that is 
why I need your advice. What would you suggest= 1) to leave the 
tissue in NF until Sunday evening and start processing, =r 2) to 
keep the fixation time (24 hours) and leave the tissue in alcohol 
70% until Sunday evening and then start processing. 
Thanks for your advice. 
Kind regards, 
Wim. 
Prof. dr. Wim Van den Broeck, DVM, MSc, PhD 
Cell biology and Histology 
Department of Morphology - Faculty of Veterinary Medicine 
Ghent University 
Salisburylaan 133, B-9820 Merelbeke, BELGIUM 
Please consider the environment before printing this e-mail. 
This e-mail, facsimile, or letter and any files or 
attachments 
transmitted =ith it contains 
information that is confidential and privileged. This information 
is 
intend=d only for the 
use of the individual(s) and entity(ies) to whom it is addressed. 
If 
you ar= the intended 
recipient, further disclosures are prohibited without 
proper 
authorization.=f you are not 
the intended recipient, any disclosure, copying, printing, or use 
of 
this i=formation is 
strictly prohibited and possibly a violation of federal or state 
law 
and re=ulations. If you 
have received this information in error, please notify 
Children's 
Medical C=nter Dallas 
immediately at 214-456-4444 or via e-mail at 
privacy@childrens.com. 
Childre='s Medical 
Center Dallas and its affiliates hereby claim all 
applicable 
privileges rel=ted to this 
information. 
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References 

1. 
3D"http://lists.utsouthwestern.edu/mailman/listin_______________________ 
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From BSullivan <@t> shorememorial.org  Fri May 21 05:48:40 2010
From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org)
Date: Fri May 21 05:50:50 2010
Subject: [Histonet] possible AFB contaminant
In-Reply-To: <5392DB699B157E4C8E65B3779634BD7D011AD985@uchmbx04-hpk03s.UCHAD.uchospitals.edu>
Message-ID: 

My first question would be what are you using for  a control? I know this
sounds strange but I have, in the past, had a problem with commercially
made ones. The AFB actually came away from the material and applied itself
to the patient slide. Crazy huh? This caused a real mess until we figured
it out and changed vendors. Also if you are doing them by hand you need to
filter after each use. Hope this helps. There is a definate difference in
appearance between a patient positive result and a loose organism. Hope
this helps.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


                                                                           
                                                               
             Sent by:                                                   To 
             histonet-bounces@          
             lists.utsouthwest                                          cc 
             ern.edu                                                       
                                                                   Subject 
                                       [Histonet] possible AFB contaminant 
             05/20/2010 03:41                                              
             PM                                                            
                                                                           
                                                                           
                                                                           
                                                                           




Please help, we have had an increase in demands for repeats on our AFB
stain. Our pathologists have been noticing what they believe are "false
positives" on our slides. We perform our AFBs on the Ventana Nexus and some
are done by hand. Because of this problem, we have been doing side by side
comparisons using the Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits, tested
our DI water sytem and have different techs cutting using different
waterbaths.
Any advice or comments would be greatly appreciated.

Thanks Debi

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From k.r.gillinder <@t> newcastle.ac.uk  Fri May 21 07:00:01 2010
From: k.r.gillinder <@t> newcastle.ac.uk (Kevin Gillinder)
Date: Fri May 21 07:00:20 2010
Subject: [Histonet] Nissl Stain P0 Mouse Head
Message-ID: 

Hi,

I am having some trouble with Nissl staining newborn (P0) mouse heads/hindbrain.
The nissl stain appears very faint in the centre of my sections. (Paraffin embedded, 7um thick)

Is this likely to be a penetration issue?
I fixed in 4% PFA  O/N before processing - Would that affect it?
I left the skull intact, but removed the skin - should I have removed some of the skull?

Any help appreciated!!!!!!

Regards,

--
Kevin

From sharon.willman <@t> bms.com  Fri May 21 07:47:51 2010
From: sharon.willman <@t> bms.com (Willman, Sharon)
Date: Fri May 21 07:47:57 2010
Subject: [Histonet] Oil Red O Staining
Message-ID: <4E17A1A86498044BA9E35001C8B7C60303C638C778@ushpwbmsmmp008.one.ads.bms.com>

Hi,
Other than using plus slides and air drying, what other techniques do you use to help frozen section tissues for Oil Red O to adhere to the slides?  How long do you usually air dry slides for cryostat sectioning?
Thanks in advance for your input.
Sharon


________________________________
This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited.
From sgoebel <@t> xbiotech.com  Fri May 21 08:56:25 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Fri May 21 08:56:30 2010
Subject: [Histonet] weekend fixation
Message-ID: <20100521065625.9e2d9aa830e8449a2412eb1e4f2f067e.154ea7a47c.wbe@email04.secureserver.net>


   This  may  be true today, but I heard CAP was going to set this int   stone in the next couple of months?

   Sarah Goebel, B.A., HT (ASCP)<
   Histotechnician
   
   XBiotech USA Inc.

   <
   8201 
   Austin, Texas  78744<
   (512)386-5107

   -------- Original Message --------
   Subject: RE: [Histonet] weekend fixation
   From: "Richard Cartun" <[1]rcartun@   Date: Thu, May 20, 2010 8:07 pm
   To: <[2]LINDA.MARGRAF@childr   <[4]sgoebel@xbiotech.com>
   Cc: <[5]histonet@lists   Dear Colleagues:

   Please    keep   in   mind   that   "recommendations",  not mandates.  You can   establish  your own formalin-fixation p   At  Hartford  Hospital  I have established   needle  core  specimens.   This  includes  0.5    fixation  and  4  hours of fixation on our tissue proces   use  the  6  hour minimum for all excisional specimens.    MAXIMUM  FIXATION  TIME.   I  can show you a   immunoreactivity  and  gene  amplification  on breast    that  have  been  fixed for 1 week, 1 month, 1 year, and l   you  don't  agree with the guidelines, work with your patholo   and  establish  your  own  fixation  policy.   And,  please remember,   fixation time is only one piece to the puzzle.  In my experience, min   imizing  ischemic  time,  making sure that the tissue does not dry out
   prior  t   more impo
   Richard
   <   Director, Histology    Director, Biospecimens
   Assistant Director, Anat   Hartford Hospital
   80 Seymour Street
   Hartford, CT 06   (860) 545-1596
   (860) 545-0174 Fax
   >>> "Mike Pen   Yeah. Good luck with that one. The minimum clearl   Mike
   -----Original Message-----
   From: <   href="mailto:histonet-bounces@lists.utsouthwestern.edu">histonet-bou
   nce   [[7]mailto:histonet-bounces@lists.utsouthwestern.edu]   [8]sgoebel@xbiotech.   Sent: Thursday, May 20, 2010 3:08 PM
   To: LINDA MARGRAF
      Subject: RE: [Histonet] weekend fixation
   From what I have seen and heard you can have fixation times up to=2   hours and still be ok?
   Sarah Goebel, B.A., HT (ASCP)
   &   Histo=echnician
   XBiotech USA Inc.
   82   Austin, Texas 78744
   (5   -------- Original Message --------
   Subject: [Histon   From: "LINDA MARGRAF" <[10]LINDA.MARGRAF@childrens.com>
   Date: Thu, M   To: <[11]histonet@lists.utsouthwestern.edu>
   Histonetters:
      Dear Colleagues,
   I have    a
   lot  o   the
   effect   of cours   asked our te   paraffin. Unfort   why I need your ad   tissue in NF until Sund   keep the fixation time (24    70% until Sunday evening and the   Thanks for your advice.
   Kind regards,
   Wim.    Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
   Cell biology and Histol   Department of Morphology - Faculty of Veterinary Medicine
   Ghent   Salisburylaan 133, B-9820 Merelbeke, BELGIUM
   Please con   This e-mail, facsimi   attachments
   transmitted =ith it co   information that is confidential and privileged. This informatio   is
   intend=d only for the
   use of the individual(s) and entit   If
   you ar= the intended
   recip   proper
   authorizati   the intended recipient, any disclosure, copying, pr   of
   this i=formation is
   strictly prohibited and   law
   and re=ulations. If   have received this information in error, please notify
   Childre   Medical C=nter Dallas
   immediately at 214-456-4444 or via e-ma   [12]privacy@childrens.com.
   Childre='s Medical
   Center Dallas and its affiliates hereby cla   applicable
   privileges rel=ted to this
   information.
   _______________________________________________
   Histonet mailing list    [13]Histonet@lists.uts   [1][14]http://lists.utsouthwestern.edu/mailman/li   stinfo/histonet<=r>;
   References
   1.
   3D"http://lists.utsouthwestern.edu/mailman/listin_____________________
   __    ________________________
   Histonet mailing list
   [15]Histonet@lists.utsouthwestern.edu    [16]h   _______________________________________________
   Histonet mailing list   [17]Histonet@lists.ut   [18]http://lists.utsouthwestern.edu/mailman/listinfo/histon
      

References

   1. 3D"mailto:rcartun@harthosp.org"
   2. 3D"mailto:LINDA.MARGRAF@childrens.com"
   3. 3D"mailto:mpence@grhs.net"
   4. 3D"mailto:sgoebel@xbiotech.com"
   5. 3D"mailto:histonet@lists.utsouthwestern.edu"
   6. 3D"mailto:mpence@grhs.net"
   7. 3D"mailto:histonet-bounces@list   8. 3D"mailto:sgoebel@xbiotech.com"
   9. 3D"mailto:histonet@lists.utsouthwestern.edu"
  10. 3D"mailto:LINDA.  11. 3D"mailto:histonet@lists.utsouthwe  12. 3D"mailto:privacy@childrens.com"
  13. 3D"mailto:Histonet@lists.utsouthwestern.edu"
  14. 3D"http://lists.utsouthwestern.edu/mailm  15. file://localhost/tmp/3D"mailt  16. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
  17. 3D"mailto:Histonet@lists.utsouthwestern.edu"
  18. 3D"http://lists.utsouthwestern.edu/mailman
From sgoebel <@t> xbiotech.com  Fri May 21 08:57:30 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Fri May 21 08:57:35 2010
Subject: [Histonet] Nissl Stain P0 Mouse Head
Message-ID: <20100521065730.9e2d9aa830e8449a2412eb1e4f2f067e.056091efcc.wbe@email04.secureserver.net>


   Did you decal the skull?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician
   
   XBiotech USA Inc.

   8201 Eas
   
   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] Nissl Stain P0 Mouse Head
   From: Kevin Gillinder <[1]   Date: Fri, May 21, 2010 5:00 am
   To: "Histonet@lists.utsouthwestern.edu"
   <[2]Histonet@lists.uts   Hi,
   I  am  having  some  trouble  with  Nissl  staining newborn (P0) mouse
   heads/hindb   The  nissl  stain  appears  very  faint  in the centre of my sections.
   (Paraffin    Is this likely to be a penetration issue?
   I fixed in 4% PFA O/N before processing - Would that affect it?
   I  left the skull intact, but removed the skin - should I have removed
   some    Any help appreciated!!!!!!
   Regards,
   --
   Kevin
   _______________________________________________
   Histonet mailing list
   [3]Histonet@lists.utsouth   [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
References

   1. 3D"mailto:k.r.gillinder@newcastle.ac.uk"
   2. 3D"mailto:Histonet@lists.utsouthwestern.edu"
   3. 3D"mailto:Histonet@lists.utsouthwestern.edu"
   4. 3D"http://lists.utsouthwestern.edu/mailman/listin
From jcampbell <@t> vdxpathology.com  Fri May 21 09:17:33 2010
From: jcampbell <@t> vdxpathology.com (Jennifer Campbell)
Date: Fri May 21 09:17:46 2010
Subject: [Histonet] recommendation for C-kit antibody
Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF838744B@VDXSERVER01.vdxpathology.local>

I'm looking for a C-kit antibody for IHC on FFPE K9 and Fel tissue.
I've only found a couple of vendors that meet those requirements.  Can
anyone recommend one to me?
 
Thanks,
 
Jennifer Campbell
From srwilkes <@t> gmail.com  Fri May 21 09:41:54 2010
From: srwilkes <@t> gmail.com (Steven Wilkes)
Date: Fri May 21 09:41:58 2010
Subject: [Histonet] DOG1 (c-kit neg. GIST tissue)
Message-ID: 

Hello

We are interested in validating DOG1, but while the literature states 5-15%
of GIST cases are c-kit negative, we are unable to obtain any.  We currently
use CD117, but would add DOG1 to our library if we could validate that it is
a more sensitive marker - and to do so we'd need c-kit negative GIST
tissue.  Does anyone have a c-kit negaitve GIST case on hand or know of a
company that sells such tissue?  Thanks in advance

Steven
srwilkes@gmail.com
From alisha <@t> ka-recruiting.com  Fri May 21 09:47:37 2010
From: alisha <@t> ka-recruiting.com (Alisha Dynan)
Date: Fri May 21 09:47:24 2010
Subject: [Histonet] High Paying Histology Jobs
Message-ID: <1173107169.1274453256949.JavaMail.cfservice@sl4app3>



Dear Histonet Members,









I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm.  I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities?  We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. 





Great Histotech Day Shift Job:


I am currently working with a client in rural Central New York in Otsego County.  This teaching hospital has a very strong academic program and affiliation with one the top universities in the state. My client is currently expanding their laboratory and looking for qualified Histotechnologists with valid NYS license (If you do not have one, please let me know and I can assist you in apply for one). My client is looking for candidates for 2 day shift positions that he has available. This hospital system offers a very competitive base salary/hourly rate, shift differentials, an outstanding benefits and retirement package, and relocation assistance, if needed. 

Great Histology Supervisor Day Shift Job:



I am currently working with a client in central Georgia.  This 500+ bed teaching hospital has a very strong academic program.  My client is currently looking for a qualified Histology Supervisor, with at least 5 years experience in histology and preferably experience as a supervisor or lead technologist. This hospital system offers a very competitive base salary/hourly rate, shift differentials, an outstanding benefits and retirement package, and relocation assistance, if needed. 


If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is.  







If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future.  I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country.  

To view some additional opportunities please visit our website at www.ka-recruiting.com.  




















Sincerely,

 

Alisha (Taylor) Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

alisha@ka-recruiting.com

www.ka-recruiting.com

 






From Betsy.Hoffman <@t> comphealth.com  Fri May 21 10:16:15 2010
From: Betsy.Hoffman <@t> comphealth.com (Betsy Hoffman)
Date: Fri May 21 10:16:44 2010
Subject: [Histonet] HOT JOB!  Histology Supervisor needed in Dallas, TX 
Message-ID: <585C6608F313C34D8979DD7A9CD38DFA056CB228@vslcexmbp02.mychg.com>

Hi..

Thought you'd be interested in seeing this HOT Histotech job opportunity currently available through CompHealth.  If you want to find out more...contact me!!

Histology Supervisor needed in Dallas, TX
This leading anatomic pathology practice located in Dallas, Texas is seeking an experienced Histology Supervisor to cover their overnight shift.   If you have a BS degree in Biological/Physical sciences or equivalent combination of education and experience; HT (ASCP) or HTL (ASCP) certification;  and 3-6 years Histology experience, with 1-2 years in a leadership role we want to talk to you!
Responsible for the day-to-day operations of the Histology laboratory and supervision of
the technical and support staff. In conjunction with the Department Manager, ensures that
all departmental policies and procedures meet the standards of current state and federal
regulations.
Night Shift: 10p - 7a
**NO TRAVELERS PLEASE!**


**Who do you know??
If this doesn't appeal to you...maybe you know someone else who's looking....refer them to me and we'll pay you a referral fee.

Betsy Hoffman
Search Consultant, Lab Sciences
CompHealth Permanent Placement
6451 North Federal Highway, Suite 702
Ft. Lauderdale, FL 33308
Direct: 1-954-837-2622| Office:  1-866-782-9029, X2622| Fax: 1-800-420-2329
betsy.hoffman@comphealth.com


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This is a commercial email from CompHealth.  If you do not want to receive future emails from CompHealth, please reply to the sender of this email and ask to be removed.





From dellav <@t> musc.edu  Fri May 21 12:39:24 2010
From: dellav <@t> musc.edu (Della Speranza, Vinnie)
Date: Fri May 21 12:39:31 2010
Subject: [Histonet] RE: possible AFB contaminant
In-Reply-To: <5392DB699B157E4C8E65B3779634BD7D011AD985@uchmbx04-hpk03s.UCHAD.uchospitals.edu>
References: <5392DB699B157E4C8E65B3779634BD7D011AD985@uchmbx04-hpk03s.UCHAD.uchospitals.edu>
Message-ID: 


Do you use DI water in your flotation baths ?

If your DI system is not already so equipped, install a 0.5 micron filter where you withdraw the water and be sure to change this filter monthly.

I'm willing to bet the bugs are on the slides before the stain is performed 


Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra.Ortiz@uchospitals.edu
Sent: Thursday, May 20, 2010 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] possible AFB contaminant

Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are "false positives" on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths.
Any advice or comments would be greatly appreciated.
 
Thanks Debi

********************************************************************************
This e-mail is intended only for the use of the individual or entity to which
it is addressed and may contain information that is privileged and confidential.
If the reader of this e-mail message is not the intended recipient, you are 
hereby notified that any dissemination, distribution or copying of this
communication is prohibited. If you have received this e-mail in error, please 
notify the sender and destroy all copies of the transmittal. 

Thank you
University of Chicago Medical Center 
********************************************************************************

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From classicdoc <@t> gmail.com  Fri May 21 13:19:28 2010
From: classicdoc <@t> gmail.com (Douglas Gregg)
Date: Fri May 21 13:19:37 2010
Subject: [Histonet] Immunostaining for West Nile virus in old paraffin blocks
Message-ID: 

I have developed two avidin-biotin alkaline phosphatase stains for
West Nile virus in tissues that are formalin fixed and paraffin
embedded. One uses a polyclonal and the other a monoclonal (7H2)
antibody. Both work fine in positive control chicken embryo heart
tissue. I have had difficulty in finding antigen in West Nile infected
brain tissues collected one year ago even though there is severe
encephalitis present.  It is possible that the tissue was collected
too late in disease. It was 10-14 days post exposure. However, I am
concerned that the negative results may be  due to the age of the
tissues in paraffin rather than having been collected too late in the
disease.  I have a good retrieval method and the tests are very
reliable on acute cases that are formalin fixed for up to one month
and paraffin embedded.

Has anyone done any retrospective studies on paraffin blocks of West
Nile virus infected  tissues as old as a year or more.If so, did they
stain? Also, I would very much like to find a paraffin block that is
one year old for a control. A crow heart would be a good choice. I am
sure there must be labs out there that have done testing of crows or
other animals found dead and submitted for testing. I would like to
try my staining on one that was collected a year ago. If it stains,
then it is not likely due to the age of the tissues in paraffin.

Help would be most appreciated if anyone can spare an old paraffin
block of a known case.

Douglas Gregg DVM PhD
Veterinary Pathologist
Southold, NY

From sgoebel <@t> xbiotech.com  Fri May 21 13:30:10 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Fri May 21 13:30:27 2010
Subject: [Histonet] Polymers
Message-ID: <20100521113010.9e2d9aa830e8449a2412eb1e4f2f067e.481759374b.wbe@email04.secureserver.net>


   Has  anyone  ever  used  Golden  Bridge  International before?  I    trying  to  find  a  secondary  polymer  (HRP)  for mouse tissue and a
   primary    proteins.   before.  Any f
   Sarah Goebel, 
   Histotechnician
   <
   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 Suite 100
   Austin, Texas  78744
   (512)386-5107
From Bill.Tench <@t> pph.org  Fri May 21 14:01:34 2010
From: Bill.Tench <@t> pph.org (Tench, Bill)
Date: Fri May 21 14:01:43 2010
Subject: [Histonet] afb contamination
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863121@MAIL1.pph.local>

We had a problem with contamination on our AFB stains, and we discovered
that it was the control slide flaking off in the copland jar which was
being used for staining the control and target slide at the same time
(makes sense as a real "control').  We identified these contaminants
because they were frequently large clusters (by "large" I would say 4-8
organisms) which we almost never see in real cases, and fortunately,
they were also not in the same plane of focus (but that can be subtle).
they did create problems.  Our solution was to stain the control
separately from the case.  No more problems. 

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

Bill.Tench@pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


mail2.pph.org made the following annotations
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Confidential E-Mail:  This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure.  Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited.  If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies.
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From Jackie.O'Connor <@t> abbott.com  Fri May 21 14:54:00 2010
From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor)
Date: Fri May 21 14:54:34 2010
Subject: [Histonet] afb contamination
In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD02863121@MAIL1.pph.local>
References: <2820431BF953BB4DA3E9E1A5882265FD02863121@MAIL1.pph.local>
Message-ID: 

You can eliminate the crossover contamination by performing the stain 
horizontally on a staining rack.  A lot of labs re-use the carbol fuchsin, 
which can have lots of extraneous AFB floating around, waiting patiently 
to attach itself to an unsuspecting patient slide.  Using the staining 
rack, you don't use a lot of reagent, and you never cross-contaminate.




From:
"Tench, Bill" 
To:
histonet@lists.utsouthwestern.edu
Date:
05/21/2010 02:34 PM
Subject:
[Histonet] afb contamination
Sent by:
histonet-bounces@lists.utsouthwestern.edu



We had a problem with contamination on our AFB stains, and we discovered
that it was the control slide flaking off in the copland jar which was
being used for staining the control and target slide at the same time
(makes sense as a real "control').  We identified these contaminants
because they were frequently large clusters (by "large" I would say 4-8
organisms) which we almost never see in real cases, and fortunately,
they were also not in the same plane of focus (but that can be subtle).
they did create problems.  Our solution was to stain the control
separately from the case.  No more problems. 

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

Bill.Tench@pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


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From Montina.VanMeter <@t> pbrc.edu  Fri May 21 15:15:28 2010
From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter)
Date: Fri May 21 15:15:35 2010
Subject: [Histonet] 2010 Louisiana Society State Meeting, June 4&5
Message-ID: <4FE7FB862E90E448AE32388E759220E5025084CF@pbrcas31.pbrc.edu>

Hello Histonetters,

 

  The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.

 

  Meeting location:  The Embassy Suites Hotel

 

                                   414 Constitution Ave.

 

                                   Baton Rouge, LA  70808  

 

 

We have a block of rooms reserved for attendees at the special rate of
$99.00.  The cut-off date for reservations has been extended to May 20,
2010.   Following that date the rooms may be reserved at that rate per
availability.  The hotel will honor this rate two days prior and two
days after our meeting (please contact the Embassy Suites Hotel for
further information: 1-225-924-6566 or 1-800-Embassy).   A complimentary
hotel shuttle service is provided for those flying into Baton Rouge.

Great dining, beautiful southern plantations and gambling boats on the
Mississippi River are just a few of the attractions in the Baton Rouge
area.  Remember to ask for the Louisiana Society for Histotechnology
group when placing your reservation.  Walk-ins are always welcome!  If
you have any questions please contact:  Tina Van Meter at 225-603-0953
or vanmetmj@pbrc.edu.

 

 

Workshops:

 

 WS #1:  FISH - IT'S NOT JUST FOR DINNER!

 

     Bonnie Whitaker, HT (ASCP) - sponsored by Cell Marque

     OSU Medical Center

 

  

 

 WS #2:  What is the Tumor Registry?

 

     Cynthia Boudreaux, LPN, CTR

     Touro Infirmary

 

 

 

 WS #3:  Veterinary vs. Clinical - Which Career is Best for Me?

 

     Pam Marcum, B.S., M.S., HT, (ASCP) 

     UAMS

 

 

 

 WS #4:  Microtomy, It's About Technique!

 

     Mari Ann Mailhiot, BA, HT (ASCP)

     Leica Microsystems

 

 

 

 WS #5:  Boot Camp for Histotechs 

 

     Mari Ann Mailhiot, BS, HT (ASCP)

     Leica Microsystems

 

 

 

 WS #6:  So You Want to Know More About Things Your Lab Doesn't Do

 

    Pam Marcum, B.S., M.S., HT (ASCP), 

    UAMS

 

    Bonnie Whitaker, HT (ASCP) QIHC

    OSU Medical Center

 

          

 

  WS #7:  Dilution, Titrations, and Good Pipetting in the IHC Laboratory

 

    Robin Simpkins, HT (ASCP) 

    Biocare Medical

 

  

 

  WS #8:  Detection Chemistry & Antibody Production

 

    Tania Ewing-Finchem, HT (ASCP)


    Ventana Roche

 

 

 

Hope to see you in June!

 

 

Tina

 

LSH Secretary

 

 

 

 

From sgoebel <@t> xbiotech.com  Fri May 21 16:06:40 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Fri May 21 16:06:44 2010
Subject: [Histonet] Eosin in Processor and iHC
Message-ID: <20100521140640.9e2d9aa830e8449a2412eb1e4f2f067e.cba83845a9.wbe@email04.secureserver.net>


   Has  anyone  had  any  trouble prestaining tissues in eosin and then    doing  IHC?   I'm  making  cell  blocks from clear fluid full of cells
   embe   be  able  to s   in the cells be   else saw an issu
   =)

   <
   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician
   XBiotech   8201 East Riverside Dr. Bl   Austin, Texas  7   (512)386-5107
From rsrichmond <@t> gmail.com  Sat May 22 13:37:31 2010
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Sat May 22 13:37:35 2010
Subject: [Histonet] Re: possible AFB contaminant
Message-ID: 

Contaminant acid-fast bacilli (AFB) on tissue section slides can
usually be identified as such, because they're outside of the plane of
the tissue section.

The organism is a nearly non-pathogenic AFB called Mycobacterium
gordoneae or Mycobacterium aquae.

The problem affects both fluorescent and the old (and obsolete) light
microscopic techniques.

This problem underlines the importance of histotechnologists' knowing
how to interpret special stain control slides. I don't recall ever
having seen a publication directed at histotechnologists that clearly
explained how to identify acid-fast bacilli in tissue sections.

Bob Richmond
Samurai Pathologist
Knoxville TN

From amosbrooks <@t> gmail.com  Sat May 22 16:43:39 2010
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Sat May 22 16:43:45 2010
Subject: [Histonet] Eosin vs IHC & Golden Bridge
Message-ID: 

Hi Sarah,
     The eosin is washed out when you deparaffinize and re-hydrate the
slides. If you are doing anything with the tissue without rehydrating, it
could cause an autofluorescence problem, but as long as you rehydrate well,
you should be fine.
     Incidentally, since you also asked about Golden Bridge International,
they seem like a nice company. They offer free samples of their products, as
long as you are willing to pay shipping. I tried their Goat polymer like
this. It is worth noting however that there are a lot of anti mouse polymers
that will work, so you should have quite a few choices available to you. If
you need some suggestions drop me an email off list.

Have a nice weekend,
Amos


Message: 7
Date: Fri, 21 May 2010 14:06:40 -0700
From: sgoebel@xbiotech.com
Subject: [Histonet] Eosin in Processor and iHC
To: histonet@lists.utsouthwestern.edu
Message-ID:
       <
20100521140640.9e2d9aa830e8449a2412eb1e4f2f067e.cba83845a9.wbe@email04.secureserver.net
>

Content-Type: text/plain; charset="utf-8"


  Has  anyone  had  any  trouble prestaining tissues in eosin and then
 doing  IHC?   I'm  making  cell  blocks from clear fluid full of cells
  embe   be  able  to s   in the cells be   else saw an issu
  =)

  <
  Sarah Goebel, B.A., HT (ASCP)

  Histotechnician
  XBiotech   8201 East Riverside Dr. Bl   Austin, Texas  7   (512)386-5107
From bakevictoria <@t> gmail.com  Mon May 24 07:23:14 2010
From: bakevictoria <@t> gmail.com (Victoria Baker)
Date: Mon May 24 07:23:19 2010
Subject: [Histonet] Moving across the country...
In-Reply-To: <14908CEF585B4206BECEF866C5726324@prueggihctechlt>
References: <5658CBDB9EAE6545ABE50D2563D81BF83872A7@VDXSERVER01.vdxpathology.local>
	<14908CEF585B4206BECEF866C5726324@prueggihctechlt>
Message-ID: 

Jennifer,

If you are looking in NY - you will need to get a license through the State
Department of Education.  Some NJ jobs will also ask for NY license as
well.  Not knowing what your educational background is, it is something I
would get at as soon as possible to see what you may need.

The general website is www.op.nysed.gov

Good luck on your exam.

Vikki

On Tue, May 18, 2010 at 9:29 AM, Patsy Ruegg  wrote:

> Jennifer,
>
> You should try some of the job placement agencies, they are always looking
> for techs to fill jobs and there is no cost to you.  I bet you will be
> contacted just from placing this post on Histonet.
>
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer
> Campbell
> Sent: Monday, May 17, 2010 2:13 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Moving across the country...
>
> Hi All,
>  My husband and I will be moving from California to the east coast this
> late summer/early fall for his work.....exciting but, a little scary!  I
> have started browsing possible histotech jobs available in either NY, CT
> or NJ but, its a rather daunting task when you're not familiar with the
> area or the labs out there!  And to add to the stress I am also
> currently studying for my HT certification which I will be taking in
> august....which I will hopefully pass so that I may have a little more
> luck job searching!  Does anyone have any advice for me as to where I
> could look into or know of any possibilities out there?
> Thanks for any advice you may have.
>
> Jennifer Campbell
> VDx Veterinary Diagnostics
> Davis, CA
> phone: (530) 753-4285
> fax: (530) 753-4286
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From relia1 <@t> earthlink.net  Mon May 24 08:41:19 2010
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Mon May 24 08:41:20 2010
Subject: [Histonet] RELIA Histology Job Alert 5-24-2010 Please take a look
	at some brand new opportunities!
Message-ID: 

Hi Histonetters!!
I hope everyone had a great weekend and is looking forward to a fun
filled Memorial Day Holiday weekend.
I have some great job opportunities I want to tell you about  All of
these positions are full time permanent positions with excellent pay,
benefits and relocation assistance.  
HISTOTECHNICIANS/HISTOTECHNOLOGISTS
OH - Cincinatti - Mohs Tech
AZ - Phoenix Histotech
AZ - IHC Tech
MA - North Shore - Senior Night Shift Histotech
MA - Boston - Night Shift Histotech
GA - Atlanta - Grossing Night Shift Histotech
 
If you or anyone you know might be interested in any of these positions
please contact me.  I can be reached at relia1@earthlink.net or toll
free at 866-607-3542.  I am available during the day or in the evening
to talk.  Remember I offer over 25 years of recruiting experience and
have been working exclusively in the permanent placement of histology
professionals for 8 years.  I respect your privacy - all inquiries are
confidential and I understand the complexities of making a job change.
I pride myself on being your advocate in the advancement of your career.
 
Thanks - Pam
 

Thank You!

 


Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net

www.myspace.com/pamatrelia

www.twitter.com/pamatrelia 

 

 
 
 
 
 
From PMonfils <@t> Lifespan.org  Mon May 24 09:10:37 2010
From: PMonfils <@t> Lifespan.org (Monfils, Paul)
Date: Mon May 24 09:10:46 2010
Subject: [Histonet] Eosin in Processor and iHC
In-Reply-To: <20100521140640.9e2d9aa830e8449a2412eb1e4f2f067e.cba83845a9.wbe@email04.secureserver.net>
References: <20100521140640.9e2d9aa830e8449a2412eb1e4f2f067e.cba83845a9.wbe@email04.secureserver.net>
Message-ID: <4EBFF65383B74D49995298C4976D1D5E06B57F08@LSRIEXCH1.lsmaster.lifespan.org>

There shouldn't be any problem if you are doing immunoperoxidase staining.  If you are doing fluorescence, you will have to be sure you remove all traces of eosin from the tissues since eosin itself is fluorescent.

From alisha <@t> ka-recruiting.com  Mon May 24 09:22:43 2010
From: alisha <@t> ka-recruiting.com (Alisha Dynan)
Date: Mon May 24 09:22:47 2010
Subject: [Histonet] Histology Job Opportunities
Message-ID: <1851848129.1274710963532.JavaMail.cfservice@sl4app3>


Dear Histonet Members,


I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm.  I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities?  We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. 

Great Histotech Day Shift Job:
I am currently working with a client in rural Central New York in Otsego County.  This teaching hospital has a very strong academic program and affiliation with one the top universities in the state. My client is currently expanding their laboratory and looking for qualified Histotechnologists with valid NYS license (If you do not have one, please let me know and I can assist you in apply for one). My client is looking for candidates for 2 day shift positions that he has available. This hospital system offers a very competitive base salary/hourly rate, shift differentials, an outstanding benefits and retirement package, and relocation assistance, if needed. 

Great Histology Supervisor Day Shift Job:
I am currently working with a client in central Georgia.  This 500+ bed teaching hospital has a very strong academic program.  My client is currently looking for a qualified Histology Supervisor, with at least 5 years experience in histology and preferably experience as a supervisor or lead technologist. This hospital system offers a very competitive base salary/hourly rate, shift differentials, an outstanding benefits and retirement package, and relocation assistance, if needed. 

If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is.  

If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future.  I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country.  

To view some additional opportunities please visit our website at www.ka-recruiting.com.  



Sincerely,

 

Alisha (Taylor) Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

alisha@ka-recruiting.com

www.ka-recruiting.com

 





From mtighe <@t> trudeauinstitute.org  Mon May 24 11:13:49 2010
From: mtighe <@t> trudeauinstitute.org (Mike Tighe)
Date: Mon May 24 11:14:20 2010
Subject: [Histonet] Yellow counterstain
Message-ID: <4BFA6CD9.26E4.00EE.0@trudeauinstitute.org>


I have seen Tissue sections counterstained with a yellow counterstain. I would like to use an Iron hematoxylin with this counter stain. Can anyone tell me what this is? Are there any tips/tricks to this counterstain? Thanks in advance!

Mike


From Kim.Donadio <@t> bhcpns.org  Mon May 24 11:42:19 2010
From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org)
Date: Mon May 24 11:42:28 2010
Subject: [Histonet] Yellow counterstain
In-Reply-To: <4BFA6CD9.26E4.00EE.0@trudeauinstitute.org>
Message-ID: 

Sounds like you might want Metanil Yellow. I have found it doesn't fade as 
much if you let your slides dry a little before you dehydrate and 
coverslip. 


Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



"Mike Tighe"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/24/2010 11:13 AM

To

cc

Subject
[Histonet] Yellow counterstain







I have seen Tissue sections counterstained with a yellow counterstain. I 
would like to use an Iron hematoxylin with this counter stain. Can anyone 
tell me what this is? Are there any tips/tricks to this counterstain? 
Thanks in advance!

Mike


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From sgoebel <@t> xbiotech.com  Mon May 24 11:42:31 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Mon May 24 11:42:38 2010
Subject: [Histonet] Yellow counterstain
Message-ID: <20100524094231.9e2d9aa830e8449a2412eb1e4f2f067e.529269b3a0.wbe@email04.secureserver.net>


   Metanil  yellow...and  it's  kind  of a pain in the rear.  Look    stain  for  goblet  cells in intestine and there should be a procedure
   fo   overcount
   Good Luck!!

   Sarah Goebel, B.A., HT (ASCP)
   Histotechnician
   
   [DEL: XBiotech USA Inc. :DEL] 

   
   8201
   <
   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] Yellow counterstain
   From: "Mike Tighe" <[1]mtigh   Date: Mon, May 24, 2010 9:13 am
   To: <[2]histonet@lists   I have seen Tissue sections counterstained with a yellow counterstain.
   I wo   anyone  tel   counterstain? Thanks i   Mike
   _______________________________________________
   Histonet mailing list
   [3]Histonet@lists.utsouth   [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
References

   1. 3D"mailto:mtighe@trudeauinstitute.org"
   2. 3D"mailto:histonet@lists.utsouthwestern.edu"
   3. 3D"mailto:Histonet@lists.utsouthwestern.edu"
   4. 3D"http://lists.utsouthwestern.edu/mailman/listin
From 41dmb41 <@t> gmail.com  Mon May 24 11:47:44 2010
From: 41dmb41 <@t> gmail.com (Drew Meyer)
Date: Mon May 24 11:48:07 2010
Subject: [Histonet] Yellow counterstain
In-Reply-To: <4BFA6CD9.26E4.00EE.0@trudeauinstitute.org>
References: <4BFA6CD9.26E4.00EE.0@trudeauinstitute.org>
Message-ID: <7043BE26-BDAA-419B-B0B9-8620E0299F1C@gmail.com>

We use tartrazine for a yellow counterstain (on mucin or VVG).  It  
stains quickly, so usually all you need is a few seconds.

Drew

Sent from my iPhone

On May 24, 2010, at 12:13 PM, "Mike Tighe"  
 wrote:

>
> I have seen Tissue sections counterstained with a yellow  
> counterstain. I would like to use an Iron hematoxylin with this  
> counter stain. Can anyone tell me what this is? Are there any tips/ 
> tricks to this counterstain? Thanks in advance!
>
> Mike
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From bhewlett <@t> cogeco.ca  Mon May 24 11:53:11 2010
From: bhewlett <@t> cogeco.ca (Bryan Hewlett)
Date: Mon May 24 11:53:20 2010
Subject: [Histonet] Yellow counterstain
References: <4BFA6CD9.26E4.00EE.0@trudeauinstitute.org>
Message-ID: <9107A9AF7DFC4DBBB54F4CF1826016A0@mainbox>

Mike,

There are a number of yellow counterstains commonly used, including picric 
acid.
For your purpose, I would recommend the following in order of preference;

Tartrazine (Acid yellow 23, Food yellow 4) CI 19140,
Metanil yellow (Acid yellow 31) CI 13065,
Martius yellow (Acid yellow 24) CI 10315.

Regards,

Bryan

----- Original Message ----- 
From: "Mike Tighe" 
To: 
Sent: Monday, May 24, 2010 12:13 PM
Subject: [Histonet] Yellow counterstain



I have seen Tissue sections counterstained with a yellow counterstain. I 
would like to use an Iron hematoxylin with this counter stain. Can anyone 
tell me what this is? Are there any tips/tricks to this counterstain? Thanks 
in advance!

Mike


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From rachel.elliott <@t> thermofisher.com  Mon May 24 12:28:15 2010
From: rachel.elliott <@t> thermofisher.com (Elliott, Rachel A.)
Date: Mon May 24 12:28:22 2010
Subject: [Histonet] Yellow counterstain
In-Reply-To: <7043BE26-BDAA-419B-B0B9-8620E0299F1C@gmail.com>
References: <4BFA6CD9.26E4.00EE.0@trudeauinstitute.org>
	<7043BE26-BDAA-419B-B0B9-8620E0299F1C@gmail.com>
Message-ID: <7B380BF8E6354F4994F20E095D43F171EF78A2E5@USPHO-MXVS01.amer.thermo.com>

I use 5 dips and get a great counterstain for mucin

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer
Sent: Monday, May 24, 2010 12:48 PM
To: Mike Tighe
Cc: 
Subject: Re: [Histonet] Yellow counterstain

We use tartrazine for a yellow counterstain (on mucin or VVG).  It  
stains quickly, so usually all you need is a few seconds.

Drew

Sent from my iPhone

On May 24, 2010, at 12:13 PM, "Mike Tighe"  
 wrote:

>
> I have seen Tissue sections counterstained with a yellow  
> counterstain. I would like to use an Iron hematoxylin with this  
> counter stain. Can anyone tell me what this is? Are there any tips/ 
> tricks to this counterstain? Thanks in advance!
>
> Mike
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From melissa.ribeiro <@t> brinegroup.com  Mon May 24 12:54:06 2010
From: melissa.ribeiro <@t> brinegroup.com (Melissa Ribeiro Passos)
Date: Mon May 24 12:54:12 2010
Subject: [Histonet] Histotech opportunity in Connecticut 
Message-ID: <43904A2EECEAB54D8A023931049FEA4C2CE6DB@brin-sbs01.brinegroup.local>

Brine Group is currently recruiting around a day Histotechnologist
position at a hospital-based clinical lab in southeastern Connecticut.

 

This is a 4-people lab that is growing. Routine histology, special
stains, and IHC.

 

This position is responsible for: 

 

*         Checking identification and preparing surgical specimens for
gross examination by pathologist.

*         Loading  surgical specimens in Autotechnicon and
setting/checking time setting.
Short accessions surgical specimens for frozen sections and other urgent
procedures.

*         Maintaining positive specimen identification throughout
specimen processing.

*         Cuting & embedding surgical specimens using rotary microtome.

*         Deparaffinizes cut sections and performs H&E staining.

*         Performing various special stains.

*         Examining specimens to determine that they meet established
standards.

*         Coordinating slides and pathology reports, and takes to
pathologist for diagnosis.

*         Preparing and maintains reagents, solutions and stains,
according to standard formula.

 

The ideal candidate would have at least one year of experience in
histological techniques, HT or HTL (ASCP) certification.

 

If this position describes you or someone you know, please contact me
for more details: 

 

Melissa Ribeiro Passos

(781) 272-3400 ext. 228

mribeiro@brinegroup.com

 

 

From mcauliff <@t> umdnj.edu  Mon May 24 13:08:01 2010
From: mcauliff <@t> umdnj.edu (Geoff McAuliffe)
Date: Mon May 24 13:05:47 2010
Subject: [Histonet] Yellow counterstain
In-Reply-To: <4BFA6CD9.26E4.00EE.0@trudeauinstitute.org>
References: <4BFA6CD9.26E4.00EE.0@trudeauinstitute.org>
Message-ID: <4BFAC081.8060909@umdnj.edu>

Fifteen to thirty seconds in saturated (or almost saturated), aqueous 
picric acid will do it.

Geoff

Mike Tighe wrote:
> I have seen Tissue sections counterstained with a yellow counterstain. I would like to use an Iron hematoxylin with this counter stain. Can anyone tell me what this is? Are there any tips/tricks to this counterstain? Thanks in advance!
>
> Mike
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************



From histonet.nospam <@t> vneubert.com  Mon May 24 13:08:15 2010
From: histonet.nospam <@t> vneubert.com (V. Neubert)
Date: Mon May 24 13:08:33 2010
Subject: [Histonet] Yellow counterstain
In-Reply-To: <9107A9AF7DFC4DBBB54F4CF1826016A0@mainbox>
References: <4BFA6CD9.26E4.00EE.0@trudeauinstitute.org>
	<9107A9AF7DFC4DBBB54F4CF1826016A0@mainbox>
Message-ID: <4BFAC08F.7080001@vneubert.com>

I'd use tartrazine or picric acid mixed with acetone.

From sgoebel <@t> xbiotech.com  Mon May 24 15:25:27 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Mon May 24 15:25:31 2010
Subject: [Histonet] tissue retention
Message-ID: <20100524132527.9e2d9aa830e8449a2412eb1e4f2f067e.9c348f1727.wbe@email04.secureserver.net>


   Hey  does  anyone  know  where to find the FDA and/or AAALAC/IACUC re   gulations  on  animal  tissue  retention  (mainly rodents)?  I know at
   prev   Sandlot pron   guidelines  state,     (trash) tissue longer than 
   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician
   
   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 Suite 100

   Austin, Texas  78744
   [DEL: (5   :DEL]
From foreightl <@t> gmail.com  Mon May 24 16:52:14 2010
From: foreightl <@t> gmail.com (Pat Laurie)
Date: Mon May 24 16:52:21 2010
Subject: [Histonet] histology position available in seattle
Message-ID: 

HISTOLOGY TECHNICIAN



*Looking for a Lab Environment that?*

?         Has been named a Best Place to Work two years running

   - Places quality first
   - Supports your ongoing learning and training
   - Supplies you with state-of-the art technology & equipment
   - Provides you with an open, airy and light-filled work space



Then explore opportunities with CellNetix Pathology & Laboratories, the
largest independent pathology organization in the Pacific Northwest,
experiencing continued growth.



We offer you the opportunity to make an impact and be rewarded for it.

   - Annual Bonus
   - Employee ownership
   - Exceptional 401K Plan with profit sharing bonus



*Summary*

The position is responsible for processing, embedding, cutting and staining
of tissue slides, accurate quality control, special projects, equipment
maintenance and all other duties assigned by the Supervisor. Strong cutting
skills are needed. This is your chance to put your skills to work in our new
state-of-the-art lab with a company dedicated to being a best place to work.




Shift is 12:30am-9am with a $10 per hour from 12:30-7am shift differential.
Shift starts Monday morning through Friday morning.

* *

*Education and Experience*

HT, HTL(ASCP) or HT/HTL eligible required.  College degree strongly
preferred.  A minimum of 5 years experience in Histology needed with strong
cutting skills and high attention to detail.



Please visit our website, www.cellnetix.com to see the full job description.
If interested, send your resume to careers@cellnetix.com placing the
position title in the subject line.


-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
From kblack <@t> digestivehlth.com  Mon May 24 20:16:56 2010
From: kblack <@t> digestivehlth.com (Konni Black)
Date: Mon May 24 20:17:20 2010
Subject: [Histonet] HT/ HTL opening in Florida
Message-ID: <62BBC7665A04468BBF48169220D99DE7@ACER>

I have been asked by a friend to post a fulltime job opening in St. Augustine, Florida. Must be HT or HTL with experience. Great pay and benefits. Very flexible schedule. Contact Robin Scott at 904-584-1884 or rscott@gi-associates.com 

From freckles9660 <@t> yahoo.com  Mon May 24 21:02:56 2010
From: freckles9660 <@t> yahoo.com (Karla Arrington)
Date: Mon May 24 21:03:09 2010
Subject: [Histonet] Problems w/IHC's
Message-ID: <923789.2556.qm@web112604.mail.gq1.yahoo.com>

Hello Histo's:

We are having troubles with getting our Cyclin D1 to stain for longer than one month.?I seems to whimp out after a month, so we make up fresh.? Any ideas? We are using a Dako clone. Also we are currently working up Surfactant A with little luck.? Our lung control does not stain at all while our patient panel is weak. We are doing 1:00 dilution for 30 mts and 30 mts using high pH (9.9) for retrieval.?Any help would be greatly appreciated!!

Karla Arrington
HT(ASCP)


      
From Loralee_Mcmahon <@t> URMC.Rochester.edu  Tue May 25 07:22:48 2010
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Tue May 25 07:24:08 2010
Subject: [Histonet] Problems w/IHC's
In-Reply-To: <923789.2556.qm@web112604.mail.gq1.yahoo.com>
References: <923789.2556.qm@web112604.mail.gq1.yahoo.com>
Message-ID: 

We use Surfactant from Biocare at 1/100 dilution 30 minutes incubation, but we use a lower pH retrieval 6.0 for 20 minutes at 99C. 
I haven't had any trouble with the Cyclin D from Dako, we use the Ready to Use version though.  
Hope that helps. 


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karla Arrington [freckles9660@yahoo.com]
Sent: Monday, May 24, 2010 10:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Problems w/IHC's

Hello Histo's:

We are having troubles with getting our Cyclin D1 to stain for longer than one month. I seems to whimp out after a month, so we make up fresh.  Any ideas? We are using a Dako clone. Also we are currently working up Surfactant A with little luck.  Our lung control does not stain at all while our patient panel is weak. We are doing 1:00 dilution for 30 mts and 30 mts using high pH (9.9) for retrieval. Any help would be greatly appreciated!!

Karla Arrington
HT(ASCP)



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From rsrichmond <@t> gmail.com  Tue May 25 13:06:58 2010
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Tue May 25 13:07:02 2010
Subject: [Histonet] Autotechnicon?
Message-ID: 

A recruiter, Melissa Ribeiro Passos, notes that

>>Brine Group is currently recruiting around a day Histotechnologist position at a hospital-based clinical lab in southeastern Connecticut.<< Duties would include

>>Loading surgical specimens in Autotechnicon and setting/checking time setting.<<

Are they actually using an ancient Technicon processor, or is this
just local lab jargon for a tissue processor?

Reason I ask is, I'm currently working in a lab that's using a 1964
Technicon, for processing and staining. No vacuum anywhere. It's
obvious from looking at the slides that temperature control is
inadequate, and the staining is quite capricious.

Bob Richmond
Samurai Pathologist
Knoxville TN

From mwich <@t> 7thwavelabs.com  Tue May 25 13:32:47 2010
From: mwich <@t> 7thwavelabs.com (Michele Wich)
Date: Tue May 25 13:32:57 2010
Subject: [Histonet] CD31 on rat frozen tissue
Message-ID: <62A8156F8071C8439080D626DF8C33A60100AF17@wave-mail.7thwave.local>

I realize that CD31 staining has been discussed extensively in the past
on histonet, but typically the conversation revolves around which
fixatives to use for paraffin sections or the inability to find a good
CD31 antibody for mouse tissue.

I am simply looking for a CD31 antibody that works well on frozen rat
tissue. Any recommendations would be greatly appreciated!


This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure.  If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited.  Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer
From lpaveli1 <@t> hurleymc.com  Tue May 25 13:52:10 2010
From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich)
Date: Tue May 25 13:52:27 2010
Subject: [Histonet] Autotechnicon?
In-Reply-To: 
References: 
Message-ID: <4BFBE41A.59CD.00EE.0@hurleymc.com>

Good thing you have years of experience under you belt!!  


>>> Robert Richmond  5/25/2010 2:06 PM >>>
A recruiter, Melissa Ribeiro Passos, notes that

>>Brine Group is currently recruiting around a day Histotechnologist
position at a hospital-based clinical lab in southeastern Connecticut.<<
Duties would include

>>Loading surgical specimens in Autotechnicon and setting/checking time
setting.<<

Are they actually using an ancient Technicon processor, or is this
just local lab jargon for a tissue processor?

Reason I ask is, I'm currently working in a lab that's using a 1964
Technicon, for processing and staining. No vacuum anywhere. It's
obvious from looking at the slides that temperature control is
inadequate, and the staining is quite capricious.

Bob Richmond
Samurai Pathologist
Knoxville TN

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From victor <@t> pathology.washington.edu  Tue May 25 14:22:41 2010
From: victor <@t> pathology.washington.edu (Victor Tobias)
Date: Tue May 25 14:23:00 2010
Subject: [Histonet] Autotechnicon?
In-Reply-To: 
References: 
Message-ID: <4BFC2381.6060202@pathology.washington.edu>

Bob,

It's time for you to find a new agent, to get some more modern assignments.

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=================================================
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.


On 5/25/2010 11:06 AM, Robert Richmond wrote:
> A recruiter, Melissa Ribeiro Passos, notes that
>
>    
>>> Brine Group is currently recruiting around a day Histotechnologist position at a hospital-based clinical lab in southeastern Connecticut.<<  Duties would include
>>>        
>    
>>> Loading surgical specimens in Autotechnicon and setting/checking time setting.<<
>>>        
> Are they actually using an ancient Technicon processor, or is this
> just local lab jargon for a tissue processor?
>
> Reason I ask is, I'm currently working in a lab that's using a 1964
> Technicon, for processing and staining. No vacuum anywhere. It's
> obvious from looking at the slides that temperature control is
> inadequate, and the staining is quite capricious.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>    

From Allison_Scott <@t> hchd.tmc.edu  Tue May 25 15:13:23 2010
From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D)
Date: Tue May 25 15:13:31 2010
Subject: [Histonet] Higgins Ink
Message-ID: <1872B4A455B7974391609AD8034C79FC026DFC45@LBEXCH01.hchd.local>

Hello to all in histo land.  Our PA used Higgins ink (india ink) on some
skin specimens and set the ink with 10%acetic acid soln.  After the
specimens were processed, all of the ink was gone, washed off.  We have
regular alcohols and xylene on the processor, this should not have made
the ink wash off.  Any suggestions on why it washed off, how to prevent
this from happening again.  Any help in this matter will be greatly
appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
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From Pat.Patterson <@t> propath.com  Tue May 25 15:32:04 2010
From: Pat.Patterson <@t> propath.com (Pat Patterson)
Date: Tue May 25 15:32:09 2010
Subject: [Histonet] IHC Position Dallas
Message-ID: <82C7248978CB50469FD6BA68EBBEFE670375A351@exchange.propathlab.com>

We're a High Quality, High Volume IHC lab - join the team!!

 

ProPath Dallas currently has an evening position opening for an experienced
IHC Technician.

Mon - Fri 7pm-3:30am

 

In this position you will be responsible for the evening operation of the
Immunohistochemistry laboratory, including slide preparation (paraffin and
frozen sections), manual IHC staining, antibody titer preparations, equipment
maintenance, supply and reagent inventory, and QC/QA record maintenance.
Leadership abilities preferred.  HT(ASCP) or QIHC (ASCP) desired.

 

Benefits include medical, dental, matched 401K plan, Short and Long Term
Disability insurance and more!

Check out our website for more information about ProPath   

www.propath.com            

For consideration send resume to:

 

ProPath, Human Resources

1355 River Bend Drive

Dallas, TX  75247

 

Fax: 214-237-1825

Job-Line: 214-237-01775

Email address:  jobs@propath.com

 

Call me with any questions you might have.

 

Pat Patterson, HTL(ASCP)

Supervisor, Immunohistochemistry

ProPath - The Leader in Pathology Services

1355 River Bend Drive

Dallas, TX 75247

 

214-237-1700 x 2027

214-237-1730 fax

 

 

Pat Patterson, HTL(ASCP)

Supervisor, Immunohistochemistry

ProPath - The Leader in Pathology Services

1355 River Bend Drive

Dallas, TX 75247

 

214-237-1700 x 2027

214-237-1730 fax

 

To learn more about ProPath, please visit http://www.ProPath.com

 

 

From sgoebel <@t> xbiotech.com  Tue May 25 16:43:41 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Tue May 25 16:43:50 2010
Subject: [Histonet] Higgins Ink
Message-ID: <20100525144341.9e2d9aa830e8449a2412eb1e4f2f067e.7203a50fd0.wbe@email04.secureserver.net>


   Try mordanting the ink in something like formalin aceto alcohol?<
   
   Histotechnician
   <   XBiotech USA Inc.
   8201 East Riverside Dr. Bldg 4 Suite 100
   Austin, Texas  78744
   <
   -------- Original Message --------
   Subject: [Histonet] Higgins Ink
   From: "Scott, Allison D" <[1]   Date: Tue, May 25, 2010 1:13 pm
   To: <[2]histonet@lists   Hello  to  all  in  histo land. Our PA used Higgins ink (india ink) on
   some
   skin specimens and set the ink with 10%acetic acid soln. After the
   specimens were processed, all of the ink was gone, washed off. We have
   regular  alcohols  and  xylene  on the processor, this should not have
   made
   the ink wash off. Any suggestions on why it washed off, how to prevent
   this from happening again. Any help in this matter will be greatly
   appreciated.
   Allison Scott HT(ASCP)
   Histology Supervisor
   LBJ Hospital
   Houston, Texas
   CONFIDENTIALITY NOTICE:
   If  you  have received this e-mail in error, please immediately notify
   the
   sender  by  return  e-mail  and delete this e-mail and any attachments
   from
   your computer system.
   To  the  extent  the  information  in  this e-mail and any attachments
   contain   protected  health  information  as  defined  by  the  Health
   Insurance Portability   and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160
   and    164;  or Chapter 181, Texas Health and Safety Code, it is confidential
   and/o   privileged.  This  e-mail  may  also be confidential and/or privileged
   under  <   individual or entity name   above. If you are not the intended recipient, or any authorized
   representative of the intended recipient, you are hereby notified that
   any    review, dissemination or copying of this e-mail and its attachments is
   strictly prohibited.
   _______________________________________________
   Histonet mailing list
   [3]Histonet@lists.utsouth   [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
References

   1. 3D"mailto:Allison_Scott@hchd.tmc.edu"
   2. 3D"mailto:histonet@lists.utsouthwestern.edu"
   3. 3D"mailto:Histonet@lists.utsouthwestern.edu"
   4. 3D"http://lists.utsouthwestern.edu/mailman/listin
From TMcNemar <@t> lmhealth.org  Wed May 26 05:08:25 2010
From: TMcNemar <@t> lmhealth.org (Tom McNemar)
Date: Wed May 26 05:08:31 2010
Subject: [Histonet] RE: Higgins Ink
In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFC45@LBEXCH01.hchd.local>
Message-ID: 

We use Higgins as well.  For many years we have set it with nothing more than regular white vinegar.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org
www.LMHealth.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Tuesday, May 25, 2010 4:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Higgins Ink

Hello to all in histo land.  Our PA used Higgins ink (india ink) on some
skin specimens and set the ink with 10%acetic acid soln.  After the
specimens were processed, all of the ink was gone, washed off.  We have
regular alcohols and xylene on the processor, this should not have made
the ink wash off.  Any suggestions on why it washed off, how to prevent
this from happening again.  Any help in this matter will be greatly
appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from
your computer system.

To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance Portability
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged.  This e-mail may also be confidential and/or privileged under
Texas law.  The e-mail is for the use of only the individual or entity named
above.  If you are not the intended recipient, or any authorized
representative of the intended recipient, you are hereby notified that any
review, dissemination or copying of this e-mail and its attachments is
strictly prohibited.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4061. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.

From dixonm <@t> ufl.edu  Wed May 26 07:20:35 2010
From: dixonm <@t> ufl.edu (Dixon,Maryann)
Date: Wed May 26 07:20:43 2010
Subject: [Histonet] test
Message-ID: 




MaryAnn Dixon BS, HT (ASCP)cm
Biological Scientist
Surgical Oncology
UF College of Veterinary Medicine
Phone (352) 294-4516
Email: dixonm@ufl.edu




From rjbuesa <@t> yahoo.com  Wed May 26 08:16:49 2010
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed May 26 08:16:53 2010
Subject: [Histonet] Higgins Ink
In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFC45@LBEXCH01.hchd.local>
Message-ID: <340552.12274.qm@web65702.mail.ac4.yahoo.com>

After India ink we always used acetone.
Ren? J.

--- On Tue, 5/25/10, Scott, Allison D  wrote:


From: Scott, Allison D 
Subject: [Histonet] Higgins Ink
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, May 25, 2010, 4:13 PM


Hello to all in histo land.? Our PA used Higgins ink (india ink) on some
skin specimens and set the ink with 10%acetic acid soln.? After the
specimens were processed, all of the ink was gone, washed off.? We have
regular alcohols and xylene on the processor, this should not have made
the ink wash off.? Any suggestions on why it washed off, how to prevent
this from happening again.? Any help in this matter will be greatly
appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.? This e-mail may also be confidential and/or privileged under 
Texas law.? The e-mail is for the use of only the individual or entity named 
above.? If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From ttruscot <@t> vetmed.wsu.edu  Wed May 26 09:40:32 2010
From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom)
Date: Wed May 26 09:40:40 2010
Subject: [Histonet] RE: Higgins Ink
In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFC45@LBEXCH01.hchd.local>
References: <1872B4A455B7974391609AD8034C79FC026DFC45@LBEXCH01.hchd.local>
Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB401134BAFF9CD@CVMMBX.vetmed.wsu.edu>

Hi Allison, Trying to think why it washed off, I wondered if the skin went back into formalin after the acetic acid, or did it go to alcohol, perhaps setting up an acid alcohol condition that maybe was enough to decolorize it. Tom T

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Tuesday, May 25, 2010 1:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Higgins Ink

Hello to all in histo land.  Our PA used Higgins ink (india ink) on some
skin specimens and set the ink with 10%acetic acid soln.  After the
specimens were processed, all of the ink was gone, washed off.  We have
regular alcohols and xylene on the processor, this should not have made
the ink wash off.  Any suggestions on why it washed off, how to prevent
this from happening again.  Any help in this matter will be greatly
appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
Texas law.  The e-mail is for the use of only the individual or entity named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From relia1 <@t> earthlink.net  Wed May 26 10:52:19 2010
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Wed May 26 10:52:26 2010
Subject: [Histonet] RELIA Histology Job Alert 5/26/10
Message-ID: 

Hi Histonetters!
I hope everyone is having a great day.  I have a few new jobs that I
want to tell you about.  These are new positions with some of my best
customers.  I have people working onsite that just love thier jobs so if
you are interested in looking into a new opportunity in one of these
areas then lets talk!
I have openings in:
Austin, TX  -Histology Generalist - IHC a plus
Phoenix, AZ - Histology Generalist
North Shore of Boston, MA - Dermpath experience a plus
Cape Cod, MA - Histology Generalist
Long Island,NY - IHC Specialist
Suffern, NY - Histology Generalist
 
All of these clients offer competitive salaries, benefits and relocation
assistance.  I can be reached at relia1@earthlink.net or toll free at
866-607-3542.
 

Thank You!
 
Pam Barker
President
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
Toll Free: (866)607-3542
FAX: (407)678-2788
E-mail: relia1@earthlink.net 
<>
www.myspace.com/pamatrelia 
www.twitter.com/pamatrelia

From aallison <@t> wpalabs.com  Wed May 26 10:56:02 2010
From: aallison <@t> wpalabs.com (aallison@wpalabs.com)
Date: Wed May 26 10:56:11 2010
Subject: [Histonet] =?UTF-8?Q?Autotechnicon=3F?=
Message-ID: <20100526085602.85fdaa4bc40941c5dd00a74b80284d9b.03d7430e3f.wbe@email04.secureserver.net>


   My  God  Tom, I haven't used an Autotechnicon or Ultra-Autotechnico   since  1979!   Was it an Mono or a Duo, or the old black spider?    sure  you  know what I mean Tom, 1 or 2 levels.  I guess    than  working  in China, reusing their stained slides by sca   the tissue.
   Akemi Allison BS, HT (ASCP) HTL

   Manager, Anatomical Pathology

   Western Pathology Associates

   Tele: 602.633.3800 X 306

   Fax: 602.861.3500

   Cell: 408.335.9994

   E-Mail: [1]aallison@wpalabs.com







   -------- Original Message --------
   Subject: Re:   From: Victor Tobias <[2]victor@pathology.washington.edu>
   Da   To: [3]histonet@lists.utsouthwestern.edu
   Bob,
   It   assignments.
   Victor
   Victor Tobias
   Clinical Applications Analyst
   Unive   Dept of Pathology Room BB220
   1959    Seattle, WA 98195
   [4]victor@pathology.washington.edu
   206-598-2792
   206-598-7   =====================   =======================   3D=   Privileged, confidential or patient identifiable information m   contained  in  this  message. This information is meant only for the    use
   of  the intended recipients. If you are not the intended recipient, o   r
   if the message has been addressed to you in error, do not read,
   dis   transmis   then destro   On 5/25/2010 11   > A recruiter, Melissa Ribeiro Passos,   >
   >
   >>>   Brine   Group   is   currently   recru   Histotechnologist  position  at  a  hospital-based  clinical     southeastern Connecticut.<< Duties would include
   >>&g   >
   >>> Loading surgical specimens in Autotechnicon an   time setting.<<
   >>>
   > Are they    > just loca   >
   >  Reason  I  ask  is, I'm c   1964
   > Technicon, for proces   > obvious from looking at   > inadequate, and the stainin   >
   > Bob Richmond
   > Samurai Patholo   > Knoxville TN
   >
   > _________________________________   > Histonet mailing list
   > [5]Histonet@lists.utsouthwestern.edu
   >   BR>>
   _______________________________________________
   Histonet   [7]Histo   [8]http://lists.utsouthwestern.   

References

   1. 3D"mailto:aallison@wpalabs.com"
   2. 3D"mailto:vic   3. 3D"mailto:histonet@lists.utso   4. 3D"mailto:victor@pathology.washi   5. 3D"mailto:Hist   6. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
   7. 3D"mailto:Histonet@lists.utsouthwestern.edu"
   8. 3D"http://lists.utsouthwestern.=/
From rsrichmond <@t> gmail.com  Wed May 26 13:02:45 2010
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Wed May 26 13:02:57 2010
Subject: [Histonet] Re: Higgins ink
Message-ID: 

Allison Scott HT(ASCP), Histology Supervisor, LBJ Hospital, Houston, Texas asks:

>>Our [pathologist's assistant] used Higgins ink (india ink) on some skin specimens and set the ink with 10% acetic acid solution. After the specimens were processed, all of the ink was gone, washed off. We have regular alcohols and xylene on the processor, this should not have made
the ink wash off. Any suggestions on why it washed off, how to prevent
this from happening again.<<

My guess is that you got the wrong kind of India ink - there are
several varieties for special purposes, and I don't understand this
issue very well. I've often bought my own Higgins india ink at art
supply stores, always getting the plainest variety I could, and never
had trouble with it - but I should learn the specifications better. I
never fix ink with anything - if you blot the specimen good and dry,
the india ink (or Davidson's ink) will adhere quite well. If you do
prefer to use a fixative, use dilute acetic acid - acetone is a fire
hazard, and we all know the dangers of the picric acid in Bouin's
fixative.

Bob Richmond
Samurai Pathologist
Knoxville TN

From elciba <@t> hotmail.com  Wed May 26 13:05:03 2010
From: elciba <@t> hotmail.com (ricky hachy)
Date: Wed May 26 13:05:19 2010
Subject: [Histonet] Autotechnicon?
In-Reply-To: <20100526085602.85fdaa4bc40941c5dd00a74b80284d9b.03d7430e3f.wbe@email04.secureserver.net>
References: <20100526085602.85fdaa4bc40941c5dd00a74b80284d9b.03d7430e3f.wbe@email04.secureserver.net>
Message-ID: 


Do you want to sell your old Autotechnicon ?

 

I'll buy any mono or duo Autotechnicon .
 
> From: aallison@wpalabs.com
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 26 May 2010 08:56:02 -0700
> Subject: RE: [Histonet] Autotechnicon?
> 
> 
> My God Tom, I haven't used an Autotechnicon or Ultra-Autotechnico=
> since 1979! Was it an Mono or a Duo, or the old black spider? =m
> sure you know what I mean Tom, 1 or 2 levels. I guess ='s better
> than working in China, reusing their stained slides by sca=ng off
> the tissue.
> Akemi Allison BS, HT (ASCP) HTL
> 
> Manager, Anatomical Pathology
> 
> Western Pathology Associates
> 
> Tele: 602.633.3800 X 306
> 
> Fax: 602.861.3500
> 
> Cell: 408.335.9994
> 
> E-Mail: [1]aallison@wpalabs.com
> 
> 
> 
> 
> 
> 
> 
> -------- Original Message --------
> Subject: Re:=istonet] Autotechnicon?
> From: Victor Tobias <[2]victor@pathology.washington.edu>
> Da=: Tue, May 25, 2010 12:22 pm
> To: [3]histonet@lists.utsouthwestern.edu
> Bob,
> It= time for you to find a new agent, to get some more modern
> assignments.
> Victor
> Victor Tobias
> Clinical Applications Analyst
> Unive=ity of Washington Medical Center
> Dept of Pathology Room BB220
> 1959 = Pacific
> Seattle, WA 98195
> [4]victor@pathology.washington.edu
> 206-598-2792
> 206-598-7e9 Fax
> ===================== ======================= 3D==D==
> Privileged, confidential or patient identifiable information m= be
> contained in this message. This information is meant only for the use
> of the intended recipients. If you are not the intended recipient, o r
> if the message has been addressed to you in error, do not read,
> dis=ose, reproduce, distribute, disseminate or otherwise use this
> transmis=on. Instead, please notify the sender by reply e-mail, and
> then destro=ll copies of the message and any attachments.
> On 5/25/2010 11=6 AM, Robert Richmond wrote:
> > A recruiter, Melissa Ribeiro Passos,=tes that
> >
> >
> >>> Brine Group is currently recru=ing around a day
> Histotechnologist position at a hospital-based clinical =b in
> southeastern Connecticut.<< Duties would include
> >>&g=
> >
> >>> Loading surgical specimens in Autotechnicon an=etting/checking
> time setting.<<
> >>>
> > Are they ?tually using an ancient Technicon processor, or is this
> > just loca=ab jargon for a tissue processor?
> >
> > Reason I ask is, I'm c=rently working in a lab that's using a
> 1964
> > Technicon, for proces=ng and staining. No vacuum anywhere. It's
> > obvious from looking at=e slides that temperature control is
> > inadequate, and the stainin=s quite capricious.
> >
> > Bob Richmond
> > Samurai Patholo=st
> > Knoxville TN
> >
> > _________________________________=____________
> > Histonet mailing list
> > [5]Histonet@lists.utsouthwestern.edu
> >=6]http://lists.utsouthwestern.edu/mailman/listinfo/histonet< BR>>
> _______________________________________________
> Histonet=iling list
> [7]Histo=t@lists.utsouthwestern.edu
> [8]http://lists.utsouthwestern.?u/mailman/listinfo/histonet
> 
> 
> References
> 
> 1. 3D"mailto:aallison@wpalabs.com"
> 2. 3D"mailto:vic 3. 3D"mailto:histonet@lists.utso 4. 3D"mailto:victor@pathology.washi 5. 3D"mailto:Hist 6. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
> 7. 3D"mailto:Histonet@lists.utsouthwestern.edu"
> 8. 3D"http://lists.utsouthwestern.=
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
_________________________________________________________________
Hotmail is redefining busy with tools for the New Busy. Get more from your inbox.
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From jchris36 <@t> gmail.com  Wed May 26 14:26:51 2010
From: jchris36 <@t> gmail.com (jason christian)
Date: Wed May 26 14:26:56 2010
Subject: [Histonet] Vibrotoming otoliths
Message-ID: 

I am trying to section an otolith from a small minnow species with a
vibrotome. The bone seems to be very dense and doesn't hold very well inside
any mounting media that I have tried. Does anyone have any suggestions as to
a media that will section smoothly but would be dense enough to hold the
small bone in the media while its being sectioned. Thank you in advance.

Jason
From cbarone <@t> NEMOURS.ORG  Wed May 26 17:20:03 2010
From: cbarone <@t> NEMOURS.ORG (Barone, Carol )
Date: Wed May 26 17:20:07 2010
Subject: [Histonet] Region II- extension
Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7D4A@wlmmsx01.nemours.org>

Histonetters- Region II has extended the mail-in registration deadline,
until Friday May 28th...just a few days away!  We know some people are
still awaiting travel approvals and we are doing our best to accomodate
everyone who wants to attend and take advantage of the early
registration. I went to the beach last week-end and it was really
great...So plan your own "Learn and Play" weekend at the Clarion with
us! For more info go to: www.nsh.org/content/region-ii-meeting
  for all the information
on the meeting, speakers, vendors and activities available. See you
there! C. Barone - Region II Director. 
 
PS: Have anything you want to tell me, or ask me to do  to help you
with, in your state, or at the national level? Contact me at:
cbarone@nemours.org   and...
 NSH Members...don't forget to cast your electronic ballots! It is so
very easy! Your voice will be heard!

 

From vphebe4 <@t> yahoo.com  Wed May 26 20:56:14 2010
From: vphebe4 <@t> yahoo.com (Phebe Verbrugghe)
Date: Wed May 26 20:56:18 2010
Subject: [Histonet] AlphaSMA staining
Message-ID: <168290.16818.qm@web33504.mail.mud.yahoo.com>










Hello everyone,
?
We are trying to stain?alpha smooth muscle actin (on mouse/human liver) using the A5228 Sigma antibody and are getting nuclear staining while this stain should be cytoplasmic. Does anyone have experience with nuclear staining of alphaSMA? Is there anything I can do to avoid nuclear staining or?can?anyone recommend me?another?good anti- alphaSMA antibody that works on both mouse and human formalin fixed paraffin sections?
?
Thank you very much in advance!
?
Phebe
?


      
From relia1 <@t> earthlink.net  Thu May 27 04:25:05 2010
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Thu May 27 04:25:12 2010
Subject: [Histonet] RELIA Histology Careers Bullletin Have a Safe and Happy
	Memorial Day Weekend 5-27-10
Message-ID: 

Hi Histonetters!! !

Summer is almost here already!  It seems like this year is flying by.
This weekend is Memorial Day Weekend and I want to wish you a safe and
Happy Memorial Day Weekend.  If you haven?t tried the marinade recipe
yet I hope you will get a chance to use it if you grill out this
weekend.  I am on the search for the perfect sangria recipes ? white and
red.  If you know one please send it to me!  There is nothing better
after a day at the beach than a glass of ice cold sangria.  

I also want to let you know that I have some new jobs since my last
e-mail and since they are with several of my best clients I am pretty
excited about them.  All of these positions are full time permanent
positions and my clients offer a great compensation, benefits and
relocation assistance.

Here are my current job openings:

HISTOLOGY/PATHOLOGY  MANAGEMENT
AZ ? Phoenix Histology Lab Manager

NY -  Long Island Histology Manager
CA ? Central CA Pathology Supervisor

WA ? Spokane Histology Manager



HISTOTECHS

TX ? Austin Histotechnician/Histotechnologist

TX ? Corpus Christi Histotechnician

AZ ? Phoenix Histotechnician/Histotechnologist

MD ? South of Hagerstown Histology Tech

MA ? Cape Cod Histotechnologist/Histotechnician

MA ? Boston Area night shift histotech

MA ? North Shore ? Night Shift Dermpath Histotech

GA ? Atlanta Night Shift Grossing Histotech 

NY-Orange/Rockland County Brand New Lab NYS license/Elig brand new lab
2nd shift.

NY ? Long Island ? Immunohistochemistry Specialist

CA-Los Angeles Histotechnologist afternoon shift 



If you or anyone you know might be interested in any of these positions
or want help with a job search in another area please contact me.   I
can be reached at 866-607-3542 or  
relia1@earthlink.net.  Please feel free to shoot me an e-mail to set up
a time to talk after the holiday weekend.  

 

Thanks-Pam

 

Thank You!

 


Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail:   relia1@earthlink.net
<  http://home.earthlink.net/~relia1>
  www.myspace.com/pamatrelia

  www.twitter.com/pamatrelia 




From Jessica.Vacca <@t> HCAhealthcare.com  Thu May 27 07:14:40 2010
From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com)
Date: Thu May 27 07:14:45 2010
Subject: [Histonet] Who sell Reichert Jung Microtomes
Message-ID: <938D716CD445614ABBB817517557B6F4E7EBADB6@NADCWPMSGCMS09.hca.corpad.net>

Can someone refer me to the vendor that offers these in Florida?

thanks

Jessica Vacca
Histology Supervisor
Brandon Regional Hospital
119 Oakfield Dr.
Brandon, FL 33511
8133571.6410 (office)
813.571.5169 (fax)

From rjbuesa <@t> yahoo.com  Thu May 27 09:01:31 2010
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu May 27 09:01:34 2010
Subject: [Histonet] Who sell Reichert Jung Microtomes
In-Reply-To: <938D716CD445614ABBB817517557B6F4E7EBADB6@NADCWPMSGCMS09.hca.corpad.net>
Message-ID: <729416.62353.qm@web65704.mail.ac4.yahoo.com>

Reichert?was bought by Leica? many years ago. You could try to contact the Leica Microsystems?representative in your area.
Ren? J.?

--- On Thu, 5/27/10, Jessica.Vacca@HCAhealthcare.com  wrote:


From: Jessica.Vacca@HCAhealthcare.com 
Subject: [Histonet] Who sell Reichert Jung Microtomes
To: histonet@lists.utsouthwestern.edu
Date: Thursday, May 27, 2010, 8:14 AM


Can someone refer me to the vendor that offers these in Florida?

thanks

Jessica Vacca
Histology Supervisor
Brandon Regional Hospital
119 Oakfield Dr.
Brandon, FL 33511
8133571.6410 (office)
813.571.5169 (fax)

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From akbitting <@t> geisinger.edu  Thu May 27 09:21:27 2010
From: akbitting <@t> geisinger.edu (Angela Bitting)
Date: Thu May 27 09:21:51 2010
Subject: [Histonet] Lab refrigerator
Message-ID: <4BFE47A7.2B7F.00C9.0@geisinger.edu>

I'm looking for a new lab refrigerator for my IHC reagents.
The techs would like to have shelves that pull out so the racks in the back aren't so hard to reach.
We'd also like to have a double-wide model.
Is anyone using one like this in their lab and would you tell me where you got it and the model #?

Thanks and Happy Memorial Day All!

Angie 

Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!




IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
-------------- next part --------------
BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Bitting, Angela
TEL;WORK:570-271-6844
ORG:;Histology
EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu
N:Bitting;Angela
END:VCARD

From marktarango <@t> gmail.com  Thu May 27 09:30:25 2010
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Thu May 27 09:30:29 2010
Subject: [Histonet] AlphaSMA staining
In-Reply-To: <168290.16818.qm@web33504.mail.mud.yahoo.com>
References: <168290.16818.qm@web33504.mail.mud.yahoo.com>
Message-ID: 

Hi Phebe,

I can't be sure about this since you didn't post your protocol, but
alpha-SMA is an antibody that does not require antigen retrieval.  If you're
doing some kind of retrieval, I'd suggest trying it without.

Thanks

Mark

On Wed, May 26, 2010 at 6:56 PM, Phebe Verbrugghe  wrote:

>
>
>
>
>
>
>
>
>
> Hello everyone,
>
> We are trying to stain alpha smooth muscle actin (on mouse/human liver)
> using the A5228 Sigma antibody and are getting nuclear staining while this
> stain should be cytoplasmic. Does anyone have experience with nuclear
> staining of alphaSMA? Is there anything I can do to avoid nuclear staining
> or can anyone recommend me another good anti- alphaSMA antibody that works
> on both mouse and human formalin fixed paraffin sections?
>
> Thank you very much in advance!
>
> Phebe
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From MLafrini <@t> csmlab.com  Thu May 27 09:49:58 2010
From: MLafrini <@t> csmlab.com (Michael LaFriniere)
Date: Thu May 27 09:53:12 2010
Subject: [Histonet] HT opening Greater DC/Baltimore area
Message-ID: <4BFE4E32.588C.00AF.0@csmlab.com>

Cytology Services of Maryland an Adventist Healthcare subsidiary is
accepting applications for HT,  currently a full time midnights and Part
time weekends position is available. Please follow website instructions
below to apply:
 
? Go to www.adventisthealthcare.com (
http://www.adventisthealthcare.com/ ) 
? Select ?Careers?
? Select ?Search Open Jobs?
? Advanced Search with Job ID # 301356 
? Create a ?Log - In? account (first time users only) 
? Select ?Job Title? to review the job description
? Select ?Apply Now
 
 
Michael R. LaFriniere
Executive Director
Cytology Services of Maryland (CSM)
301-206-2555 ext 27      301-206-2595 fax
michael.lafriniere@csmlab.com
From Timothy.Morken <@t> ucsfmedctr.org  Thu May 27 10:22:18 2010
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim)
Date: Thu May 27 10:22:30 2010
Subject: [Histonet] Lab refrigerator
In-Reply-To: <4BFE47A7.2B7F.00C9.0@geisinger.edu>
References: <4BFE47A7.2B7F.00C9.0@geisinger.edu>
Message-ID: <1AAF670737F193429070841C6B2ADD4C0187C919C0@EXMBMCB15.ucsfmedicalcenter.org>

Angie, 

We had exactly the same requirements and got a Helmer double fridge with glass doors, pull-out shelves and a chart temp recorder. It works very nicely. Model HLR256. We also got the upgrade to heated glass doors and temperature calibration certification. 

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting
Sent: Thursday, May 27, 2010 7:21 AM
To: histonet
Subject: [Histonet] Lab refrigerator

I'm looking for a new lab refrigerator for my IHC reagents.
The techs would like to have shelves that pull out so the racks in the back aren't so hard to reach.
We'd also like to have a double-wide model.
Is anyone using one like this in their lab and would you tell me where you got it and the model #?

Thanks and Happy Memorial Day All!

Angie 

Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!




IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.


From dholmes <@t> umc.edu  Thu May 27 10:59:42 2010
From: dholmes <@t> umc.edu (Dianne E. Holmes)
Date: Thu May 27 10:59:52 2010
Subject: [Histonet] A/O Spencer sliding microtome
Message-ID: 

Does anyone have a manual for this microtome?  I am in possession of 2 of these 'dependable giants' and would like to fix at least one of them!!  The latest casuality was one that I have used on a regular basis since 1970.  Please somebody help me get this 'friend' up and running!


Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way.

From rstewart <@t> grmc.org  Thu May 27 11:35:12 2010
From: rstewart <@t> grmc.org (Stewart, Robin P.)
Date: Thu May 27 11:35:17 2010
Subject: [Histonet] non certified histologists with OJT
Message-ID: 

Hi all,
I have been training two people to become HT's (OJT) in our facility. Our hospital is now in the process or evaluating how to classify and pay these individuals till they get their certification completed. Do others use non-certified HT's and if you do not mind how you did create a positions/description for them as trainees?  They will both be going through a program to get their certificate and plan to stay at our facility. What have others out there done in this situation?
Thanks in advance.
Robin Stewart-HT
From lizzypas <@t> operamail.com  Thu May 27 13:57:06 2010
From: lizzypas <@t> operamail.com (Elizabeth Pastore)
Date: Thu May 27 13:57:11 2010
Subject: [Histonet] Gills OG-6
Message-ID: <20100527185706.E70254FCE0@c-in3ws--03-06.sv2.lotusliveops.com>

I'm kind of puzzled.  Does anyone know the difference between the common OG-6 Pap Stain and Gill's OG-6?  Looking at vendor's MSDS doesn't help much.  Is the difference in its components or is it the method of preparation? 

Thanks,
Eliza

-- 
_______________________________________________
Surf the Web in a faster, safer and easier way:
Download Opera 9 at http://www.opera.com


From jshea121 <@t> roadrunner.com  Thu May 27 17:26:40 2010
From: jshea121 <@t> roadrunner.com (Shea's)
Date: Thu May 27 17:26:44 2010
Subject: [Histonet] New CAP question GEN.20425
Message-ID: <007EE52A242F48599877374277EFE8E5@JoannePC>

      How has your laboratory adressed the new CAP question GEN.20425 
     
       
     
            Has your laboratory or your institution developed a solution to the new CAP requirement below?

            GEN.20425

            Does the laboratory have a policy to ensure that all records, slides, blocks, and tissues are retained and available for appropriate times should the laboratory cease operation?
           
     
From sshawdfy <@t> med.kobe-u.ac.jp  Thu May 27 21:10:19 2010
From: sshawdfy <@t> med.kobe-u.ac.jp (shymaa shawadfy)
Date: Thu May 27 21:10:26 2010
Subject: [Histonet] trouble in BrdU IHC for brain samples
Message-ID: <20100528021040.33D7724AF36@smtpgate.kobe-u.ac.jp>

Dear Histonet members

 

I am having great trouble in my BrdU IHC in mice brain samples. I am trying
to establish a protocol for paraformaldehyde fixed - paraffin embedded
sections. I cut samples at 6 ?m thickness. 

Few times I got good nuclear stain but other times using the same protocol I
fail to get any signal. In addition to high background

I used microwave oven for antigen retrieval (citrate buffer) in addition 2 N
HCl for 60 min at 37 degrees.  Using 2N HCl alone produced weak signal. I
also tried combining pepsin digestion with HCl but I did not get any signal
and my tissues were over digested. And now I think that I will stick to
water bath at 98 degrees for 40 min instead of the microwave. 

Blocking is 2 % BSA + 2% NGS in PBS T 

I used BD bioscience anti BrdU  at 1:100 dilution , then biotin conjugated
anti mouse , vector ABC kit and color development with DAB with Nickel
enhancement.

 

Do you have any suggestions?

 

I read that we can also use 0.07 N NaOH to enhance the antibody reactivity;
can you please tell me the protocol? 

 

 Thank you 

 

Shymaa 

From PMonfils <@t> Lifespan.org  Fri May 28 09:30:42 2010
From: PMonfils <@t> Lifespan.org (Monfils, Paul)
Date: Fri May 28 09:30:48 2010
Subject: [Histonet] Higgins Ink
In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFC45@LBEXCH01.hchd.local>
References: <1872B4A455B7974391609AD8034C79FC026DFC45@LBEXCH01.hchd.local>
Message-ID: <4EBFF65383B74D49995298C4976D1D5E06B5858E@LSRIEXCH1.lsmaster.lifespan.org>

I was surprised to see all the references to "setting" or "mordanting"
of India ink.  I have used Higgins ink for many years (No. 4415
Waterproof Drawing Ink). I just blot the tissue, apply the ink, wait a
few seconds, blot the excess ink, and drop the tissue back into formalin
or alcohol. I have never had a problem with the ink washing off.


From Montina.VanMeter <@t> pbrc.edu  Fri May 28 11:37:57 2010
From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter)
Date: Fri May 28 11:38:59 2010
Subject: [Histonet] 2010 Louisiana Society for Histotechnology State Meeting
Message-ID: <4FE7FB862E90E448AE32388E759220E5025D28E2@pbrcas31.pbrc.edu>

Hello Histonetters,

 

The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.

 

 

  Meeting location:  The Embassy Suites Hotel

                                   414 Constitution Ave.

                                   Baton Rouge, LA  70808  

 

It's not too late!  Walk-ins are always welcome!  

 

 

We have a block of rooms reserved for attendees at the special rate of
$99.00.  The cut-off date for reservations has been extended to May 20,
2010.   Following that date the rooms may be reserved at that rate per
availability.  The hotel will honor this rate two days prior and two
days after our meeting (please contact the Embassy Suites Hotel for
further information: 1-225-924-6566 or 1-800-Embassy).   A complimentary
hotel shuttle service is provided for those flying into Baton Rouge.

 

Great dining, beautiful southern plantations and gambling boats on the
Mississippi River are just a few of the attractions in the Baton Rouge
area.  Remember to ask for the Louisiana Society for Histotechnology
group when placing your reservation.  If you have any questions please
contact:  Tina Van Meter at 225-603-0953 or vanmetmj@pbrc.edu.

 

 

Workshops:

 

  WS #1:  FISH - IT'S NOT JUST FOR DINNER!

 

     Bonnie Whitaker, HT (ASCP) - sponsored by Cell Marque

     OSU Medical Center

 

  

 

 WS #2:  What is the Tumor Registry?

 

     Cynthia Boudreaux, LPN, CTR

     Touro Infirmary

 

 

 

 WS #3:  Veterinary vs. Clinical - Which Career is Best for Me?

 

     Pam Marcum, B.S., M.S., HT, (ASCP)

     UAMS

 

 

 

 WS #4:  Microtomy, It's About Technique!

 

     Mari Ann Mailhiot, BA, HT (ASCP)

     Leica Microsystems

 

 

 

 WS #5:  Boot Camp for Histotechs 

 

     Mari Ann Mailhiot, BS, HT (ASCP)

     Leica Microsystems

 

 

 

 WS #6:  So You Want to Know More About Things Your Lab Doesn't Do

 

    Pam Marcum, B.S., M.S., HT (ASCP), 

    UAMS

 

    Bonnie Whitaker, HT (ASCP) QIHC

    OSU Medical Center

 

          

 

  WS #7:  Dilution, Titrations, and Good Pipetting in the IHC Laboratory

 

    Robin Simpkins, HT (ASCP)  

    Biocare Medical

 

  

 

  WS #8:  Detection Chemistry & Antibody Production

 

    Tania Ewing-Finchem, HT (ASCP)  

    Ventana Roche

 

 

 

 

 

Hope to see you in June!

 

Tina Van Meter

LSH Secretary

 

 

From gagnone <@t> KGH.KARI.NET  Fri May 28 13:37:42 2010
From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric)
Date: Fri May 28 13:37:50 2010
Subject: [Histonet] Lab Refrigerator
Message-ID: 

Ours is a Sanyo Medical MPR-1013 R double glass-door model with both pullout and stationary shelves.  We looked at buying a second-hand unit, but were able to buy brand-new.  We call it our "dream fridge" having made do with some older, much less satisfactory ones before the Sanyo arrived.  It holds all our immuno supplies, and the pullout drawers make finding antibodies much easier.
 
Hope this helps,
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital
Kingston, Ontario, Canada
 
 


From Valerie.Hannen <@t> parrishmed.com  Fri May 28 14:40:54 2010
From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie)
Date: Fri May 28 14:41:42 2010
Subject: [Histonet] New CAP question GEN.20425
References: <007EE52A242F48599877374277EFE8E5@JoannePC>
Message-ID: <5680DA93771F0C48954CC8D38425E72401AD03C1@ISMAIL.parrishmed.local>

We have addressed and answered this question by including the following statement on the bottom of our "Period of Retention" List :
 
" All the above mentioned will be sent to a facility (hospital) approved storage site in the event that the Clinical Laboratory or hospital closes."
 
We had our CAP inspection earlier this year, and this statement was accepted by our inspector.
 
Valerie Hannen, MLT (ASCP), HTL,SU (FL)
Parrish Medical Center
Titusville, Florida

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Shea's
Sent: Thu 5/27/2010 6:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] New CAP question GEN.20425



      How has your laboratory adressed the new CAP question GEN.20425
    
      
    
            Has your laboratory or your institution developed a solution to the new CAP requirement below?

            GEN.20425

            Does the laboratory have a policy to ensure that all records, slides, blocks, and tissues are retained and available for appropriate times should the laboratory cease operation?
          
    
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From pruegg <@t> ihctech.net  Sat May 29 10:45:45 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sat May 29 10:46:24 2010
Subject: SPAM-LOW:  [Histonet] trouble in BrdU IHC for brain samples
In-Reply-To: <20100528021040.33D7724AF36@smtpgate.kobe-u.ac.jp>
References: <20100528021040.33D7724AF36@smtpgate.kobe-u.ac.jp>
Message-ID: 

I have a really good protocol for BRDU on rat samples using Novacastra mouse
anti BRDU, but I have not tried it on mice tissue.  Contact me again next
week at work (Tuesday) and I can send it to you.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of shymaa
shawadfy
Sent: Thursday, May 27, 2010 8:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] trouble in BrdU IHC for brain samples

Dear Histonet members

 

I am having great trouble in my BrdU IHC in mice brain samples. I am trying
to establish a protocol for paraformaldehyde fixed - paraffin embedded
sections. I cut samples at 6 ?m thickness. 

Few times I got good nuclear stain but other times using the same protocol I
fail to get any signal. In addition to high background

I used microwave oven for antigen retrieval (citrate buffer) in addition 2 N
HCl for 60 min at 37 degrees.  Using 2N HCl alone produced weak signal. I
also tried combining pepsin digestion with HCl but I did not get any signal
and my tissues were over digested. And now I think that I will stick to
water bath at 98 degrees for 40 min instead of the microwave. 

Blocking is 2 % BSA + 2% NGS in PBS T 

I used BD bioscience anti BrdU  at 1:100 dilution , then biotin conjugated
anti mouse , vector ABC kit and color development with DAB with Nickel
enhancement.

 

Do you have any suggestions?

 

I read that we can also use 0.07 N NaOH to enhance the antibody reactivity;
can you please tell me the protocol? 

 

 Thank you 

 

Shymaa 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From pruegg <@t> ihctech.net  Sat May 29 10:48:41 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sat May 29 10:49:27 2010
Subject: SPAM-LOW:  Re: [Histonet] AlphaSMA staining
In-Reply-To: 
References: <168290.16818.qm@web33504.mail.mud.yahoo.com>
	
Message-ID: <178D23D7F6F347258DDA283136EFD693@prueggihctechlt>

Phebe,

It does sound like you are over heating or digesting so I agree with Mark
try this without any antigen retrieval.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Thursday, May 27, 2010 8:30 AM
To: Phebe Verbrugghe
Cc: histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] AlphaSMA staining

Hi Phebe,

I can't be sure about this since you didn't post your protocol, but
alpha-SMA is an antibody that does not require antigen retrieval.  If you're
doing some kind of retrieval, I'd suggest trying it without.

Thanks

Mark

On Wed, May 26, 2010 at 6:56 PM, Phebe Verbrugghe  wrote:

>
>
>
>
>
>
>
>
>
> Hello everyone,
>
> We are trying to stain alpha smooth muscle actin (on mouse/human liver)
> using the A5228 Sigma antibody and are getting nuclear staining while this
> stain should be cytoplasmic. Does anyone have experience with nuclear
> staining of alphaSMA? Is there anything I can do to avoid nuclear staining
> or can anyone recommend me another good anti- alphaSMA antibody that works
> on both mouse and human formalin fixed paraffin sections?
>
> Thank you very much in advance!
>
> Phebe
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From amosbrooks <@t> gmail.com  Sat May 29 11:46:45 2010
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Sat May 29 11:46:51 2010
Subject: [Histonet] trouble in BrdU IHC for brain samples
Message-ID: 

Hi,
     Firstly, it is a really good idea to include a highly proliferative
organ as a control to make sure that the BrdU was successfully injected in
the first place. Intestine usually works great for this. If looking at brain
only there may actually not be any positive staining because there doesn't
happen to be any proliferation going on at the time.
     I think you are on the right track ditching the microwave. There is too
much variability and lack of control. I always use 1N HCl, but 2N might
work. I use Trypsin for retrieval as it has given me the best results so
far. I have heard of others having success with citrate HIER (heat induced
epitope retrieval) but it wasn't as good in my experience. Beyond that, I
would play with the dilution to make sure you have that nailed down. A good
control is critical though. Please drop me a message if you need any more
help. I know it can be frustrating sometimes,

Best of luck,
Amos


Message: 3
Date: Fri, 28 May 2010 11:10:19 +0900
From: "shymaa shawadfy" 
Subject: [Histonet] trouble in BrdU IHC for brain samples
To: 
Message-ID: <20100528021040.33D7724AF36@smtpgate.kobe-u.ac.jp>
Content-Type: text/plain;       charset="iso-8859-7"

Dear Histonet members



I am having great trouble in my BrdU IHC in mice brain samples. I am trying
to establish a protocol for paraformaldehyde fixed - paraffin embedded
sections. I cut samples at 6 ?m thickness.

Few times I got good nuclear stain but other times using the same protocol I
fail to get any signal. In addition to high background

I used microwave oven for antigen retrieval (citrate buffer) in addition 2 N
HCl for 60 min at 37 degrees.  Using 2N HCl alone produced weak signal. I
also tried combining pepsin digestion with HCl but I did not get any signal
and my tissues were over digested. And now I think that I will stick to
water bath at 98 degrees for 40 min instead of the microwave.

Blocking is 2 % BSA + 2% NGS in PBS T

I used BD bioscience anti BrdU  at 1:100 dilution , then biotin conjugated
anti mouse , vector ABC kit and color development with DAB with Nickel
enhancement.

Do you have any suggestions?

I read that we can also use 0.07 N NaOH to enhance the antibody reactivity;
can you please tell me the protocol?
 Thank you
Shymaa
From Laura.Miller <@t> leica-microsystems.com  Sat May 29 16:01:04 2010
From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com)
Date: Sat May 29 16:01:11 2010
Subject: [Histonet] Laura Miller is Out of  the Office.
Message-ID: 


I will be out of the office starting  05/27/2010 and will not return until
06/02/2010.

I am on vacation beginning Thursday, May 27th returning back to the office
on Wednesday, June 2nd


______________________________________________________________________
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From classicdoc <@t> gmail.com  Sun May 30 18:29:02 2010
From: classicdoc <@t> gmail.com (Douglas Gregg)
Date: Sun May 30 18:29:07 2010
Subject: [Histonet] Storage of control slides for Chlamydia
Message-ID: 

Histonetters,
Some of you surely cut control slides for Chlamydia trachomatis and
store them for use in future tests. Can you tell me how long you can
store them and under what conditions. I was thinking of cutting
paraffin sections and storing them at -20. I could gas the storage box
with CO2 to eliminate O2 but maybe that is not necessary. I would
deparaffinize them after taking them out of the freezer. Any
suggestions. Some antigens won't hold up to long term storage, but I
don't know about Chlamydia. Thanks for any help.

Douglas Gregg DVM PhD
Veterinary pathologist

From rjbuesa <@t> yahoo.com  Mon May 31 08:46:42 2010
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Mon May 31 08:46:46 2010
Subject: [Histonet] Storage of control slides for Chlamydia
In-Reply-To: 
Message-ID: <823815.24409.qm@web65715.mail.ac4.yahoo.com>

You have several options, CO2 not being one of them:
1- storage at -20?C
2- cover the sections with melted paraffin and store them like that
3- immerse them in mineral oil, or
4- hold them in nitrogen.
Ren? J.

--- On Sun, 5/30/10, Douglas Gregg  wrote:


From: Douglas Gregg 
Subject: [Histonet] Storage of control slides for Chlamydia
To: histonet@lists.utsouthwestern.edu
Date: Sunday, May 30, 2010, 7:29 PM


Histonetters,
Some of you surely cut control slides for Chlamydia trachomatis and
store them for use in future tests. Can you tell me how long you can
store them and under what conditions. I was thinking of cutting
paraffin sections and storing them at -20. I could gas the storage box
with CO2 to eliminate O2 but maybe that is not necessary. I would
deparaffinize them after taking them out of the freezer. Any
suggestions. Some antigens won't hold up to long term storage, but I
don't know about Chlamydia. Thanks for any help.

Douglas Gregg DVM PhD
Veterinary pathologist

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet