[Histonet] mouse perfusion rate

[Merced M Leiker]

Hi Joe,

Thanks for that notice about flow rates. But I think for the mouse you 
meant 1-3mls/min (not per 10min?)...

Regards,
Merced

--On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby 
<saby_joseph_a <@t> yahoo.com> wrote:

>
>
> All-
>
> From previous work with rat perfusions, the flow rate was about 10
> ml/minute.  If I had to guess, the equivalent flow rate for a mouse would
> be closer to 1-3 mls/10 minutes.  If you go 10 ml/minute, you will
> definitely cause blowout artefacts.
>
> Joe Saby, BA HT
>
>
>
>
> __________________________________________________
> From: Merced M Leiker <leiker <@t> buffalo.edu>
> To: Charles.Scouten <@t> leica-microsystems.com; making <@t> ufl.edu;
> histonet <@t> lists.utsouthwestern.edu
> Sent: Fri, March 19, 2010 9:21:38 AM
> Subject: RE: [Histonet] mouse perfusion rate
>
> The vasculature will leak too much and the mouse will get bloated -
> you'll
> see it first in either the intestines blowing up like a balloon or fluid
> coming out of the nose. Just not the same as the heart pumping when the
> mouse is alive with intact physiology and normal functioning.  Don't know
> exactly why, but that's what happens when you go too fast.  Perhaps the
> vasculature has lost its control to compensate for the pressure? I'm not
> a
> physiologist so I'm not sure why...maybe someone on the Histonet can
> answer
> that?
>
> Regards,
> Merced
>
> --On Thursday, March 18, 2010 5:49 PM -0500
> Charles.Scouten <@t> leica-microsystems.com wrote:
>
>>
>>
>> Why not?  What happens?  One would think the mammalian cardiovascular
>> system could withstand physiological pressures and flow rates, at least
>> for one lifetime?
>>
>>
>>
>>
>> Cordially,
>>
>> Charles W. Scouten, Ph.D
>>
>> Product Manager, MNL
>>
>> Biosystems Division
>>
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>>
>> Leica Biosystems Richmond, Inc.
>> 5205 Route 12
>> P.O. Box 528
>> Richmond, IL 60071
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>>
>> Telephone 630 964 0501
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>> facsimile +1 630 964 0576
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>>
>> www.leica-microsystems.com
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>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Merced M
>> Leiker <leiker <@t> buffalo.edu>
>> Sent: Thursday, March 18, 2010 12:38 PM
>> To: MKing <making <@t> ufl.edu>; histonet <@t> lists.utsouthwestern.edu
>> Subject: Re: [Histonet] mouse perfusion rate
>>
>>
>>
>> That may be mouse cardiac output, but I can assure you, from experience,
>> you do not want to perfuse at 17ml/min.
>>
>> Regards,
>> Merced
>>
>> --On Thursday, March 18, 2010 1:32 PM -0400 MKing < making <@t> ufl.edu>
>> wrote:
>>
>>> Li,
>>>
>>> Mouse cardiac output seems to be about 17 ml/min (e.g.
>>> www.transonic.com/mice1.shtml), you probably want to try for that to
>>> keep  pressures close to physiological.
>>> A syringe pump is pretty inexpensive and probably all you need.
>>>
>>> Mike
>>>
>>> ----- Original Message -----
>>> From: Li Zhang < dancingwing <@t> yahoo.com>
>>> Date: Wednesday, March 17, 2010 14:59
>>> Subject: [Histonet] question about mouse perfusion
>>> To: histonet <@t> lists.utsouthwestern.edu
>>>
>>> > > My question is: can anyone give me a rough idea of how fast I
>>> > > should inject ( like ml/min). I think I've tried like 30 ml in 3
>>> > > min, and I suspect that it's too fast because I do observe
>>> > > tissue swelling sometimes.
>>>
>>>
>>>
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>>
>>
>>
>> Merced M Leiker
>> Research Technician III
>> Cardiovascular Medicine
>> 348 Biomedical Research Building
>> State University of New York at Buffalo
>> 3435 Main St, Buffalo, NY 14214 USA
>> leiker <@t> buffalo.edu
>> 716-829-6118 (Ph)
>> 716-829-2665 (Fx)
>>
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>
>
> Merced M Leiker
> Research Technician III
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214  USA
> leiker <@t> buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
>
> No trees were harmed in the sending of this email.
> However, many electrons were severely inconvenienced.
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>



Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.