[Histonet] whole mount protocol

[Carlos G. Perez-Garcia]

hi all

i am trying to do an in situ hybridization in whole mount brains at  
P7 but is hard to get staining mostly due to permeabilization issues  
since is 7 days postnatal brain. I would really appreciate to receive  
any suggestion or protocol to help me in this issue.

Thanks a lot in advance

Carlos




Carlos G. Perez-Garcia, Ph.D.
Molecular Neurobiology Lab (MNL-O)
The Salk Institute
10010 North Torrey Pines Road
92037 La Jolla, CA, USA

Phone: 858-453-4100 ext. X1449
Fax: 858 558 6207
E-mail: cpgarcia <@t> salk.edu