From kmerriam2003 <@t> yahoo.com Mon Mar 1 06:46:09 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Mar 1 06:46:14 2010 Subject: Fw: [Histonet] frozen sections of cartilage Message-ID: <836501.4247.qm@web50303.mail.re2.yahoo.com> Hi Guys, I never received a response and I was?hoping that someone would have some tips.? We?are embedding and sectioning rat and mouse knee cartilage and I?am having trouble keeping it on the slides.? Even when I do my IHC by hand, the sections are still?lifting off.? Any suggestions? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ----- Forwarded Message ---- From: Kim Merriam To: Histonet Sent: Mon, February 22, 2010 2:37:58 PM Subject: [Histonet] frozen sections of cartilage Hi Everyone, Any tips for keeping frozen sections of cartilage from falling off the slides?during?IHC staining?? We should not have to do HIER, but they still fall of the slides quite easily. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Mon Mar 1 09:11:11 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Mon Mar 1 09:10:42 2010 Subject: [Histonet] Pathologist Assistant Jobs Message-ID: <1496304024.1267456271654.JavaMail.cfservice@webserver53> Dear Histonet Subscribers, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Laboratory Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory positions across the country. Our clients typically assist with relocation expenses. One particular client I am working with is one of the top hospital systems in the country. This hospital in Tennessee is looking for a Pathologist Assistant for their Pathology Department. My client is looking for someone who is PA(ASCP) certified and has at least 5 years experience as a pathologist assistant. This position has a very competitive base salary, great benefits, and an unparalleled retirement package. My client will also assist with relocation expenses. We are also working on a number of other opportunities. See the titles below and let me know if you would like more details! Other Current Opportunities: NV - Pathologist's Assistant 2nd shift TN - Pathologist's Assistant 1st shift (must have Master's) Midwest - Pathologist's Assistant 1st shift Southern CA - Pathologist Assistant Supervisor If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Laboratory positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From relia1 <@t> earthlink.net Mon Mar 1 09:28:38 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Mar 1 09:28:44 2010 Subject: [Histonet] RELIA Histology Careers Bulletin 03/01/10 Are you into Facebook? MySpace? Twitter? or Linkedin? Message-ID: Hello Histonetters!! Facebook, Myspace, Linkedin, Twitter; Have you joined the Social Networking Craze? If so then you know how much fun it can be reconnecting with old friends, making new ones. If you do have an account on one of these networks I would love to connect with you. Please shoot me back an e-mail with your id on the site or an invitation to connect/friend/follow and I will respond right away. If that was like a foreign language to you and you would like some help using any of these sites please let me know. I would be happy to help you join in. I also wanted to tell you about my current job openings. All of these jobs are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance. Need help with your resume or following up after the interview or negotiating salary? Give me a call or shoot me an e-mail. I would be happy to help out. This is a complimentary service of RELIA of course. Here is a list of my current open positions HISTOLOGY/PATHOLOGY MANAGEMENT MA-Cape Cod Pathology Supervisor OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA-Central CA-Pathology Supervisor HISTOTECHS FL-Miami Histotechnologist needed for growing private lab GA-Grossing Histotechnologist Night Shift Great Shift diff MA-Boston - Immunohistochemistry Specialist MA-Boston - Histotechs day and evening shift NY-Orange/Rockland County Brand New Lab NYS license or Elig 2nd shift. CA-Los Angeles Histotechnologist afternoon shift PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC ? Charlotte PA grad from NAACLES program required FL- Miami Growing private lab MARKETING AND SALES Marketing Rep - Histo/Cyto Products Pacific NW Region CYTOLOGY PA-Pittsburgh Cytology Supervisor If you or any of your friends would like more information on any of the positions listed or help with a job search in another area please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember it never hurts to look Hope to hear from you soon. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia From af46 <@t> buffalo.edu Mon Mar 1 09:44:51 2010 From: af46 <@t> buffalo.edu (Annette Featherstone) Date: Mon Mar 1 09:44:57 2010 Subject: [Histonet] CD31 in rats Message-ID: <000001cab956$2215a3d0$6640eb70$@edu> Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone From Anna.Hernandez <@t> crl.com Mon Mar 1 12:01:03 2010 From: Anna.Hernandez <@t> crl.com (Hernandez, Anna) Date: Mon Mar 1 12:01:07 2010 Subject: [Histonet] CD31 in rats In-Reply-To: <000001cab956$2215a3d0$6640eb70$@edu> References: <000001cab956$2215a3d0$6640eb70$@edu> Message-ID: <63EC5E7C7346EB4BA09D6DC5651E061BEA5E1E@ent-pr-xch-02.na01.crl.com> Hello Annette, We use Purified Mouse anti-Rat CD31 from BD Biosciences (Catalog# 555025). We use on frozen sections fixed with acetone for 10 minutes with the following steps: 1X Morphosave (Ventana) (15 min) 0.3% H202 quench(Sigma) (20 min) Avidin and Biotin block (Vector) (15 min each) Covance blocking reagent (25 min) Mouse anti-Rat CD31 (25 min) at 2.0 ug/mL Biotinylated Horse anti-Mouse IgG (Vector-BA-2001) (25 minutes) at 5.0 ug/mL Covance Labeling Reagent (25 min) Covance Dab (2 x 5 min) (Each step has PBS washes in between and is performed at RT) Thanks, Anna -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone Sent: Monday, March 01, 2010 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31 in rats Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Reuel.Cornelia <@t> tsrh.org Mon Mar 1 12:10:13 2010 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Mon Mar 1 12:10:28 2010 Subject: [Histonet] CD 31 on Pig Message-ID: <4B8BAEA5020000C5000719EC@mail.TSRH.ORG> Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank you. Reuel From alexandra.meinl <@t> gmail.com Mon Mar 1 12:37:31 2010 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Mon Mar 1 12:37:37 2010 Subject: [Histonet] CD 31 on Pig In-Reply-To: <4B8BAEA5020000C5000719EC@mail.TSRH.ORG> References: <4B8BAEA5020000C5000719EC@mail.TSRH.ORG> Message-ID: We use the Rabbit anti-PECAM-1 (sc-1506-R, Santa Cruz) 1:100 on paraffin sections (HIER with citrate buffer pH 6.0 required). It works on pig and rat tissue. Alexandra 2010/3/1 Reuel Cornelia > Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank > you. > > Reuel > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.meinl@trauma.lbg.ac.at From mward <@t> wfubmc.edu Mon Mar 1 12:56:17 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Mon Mar 1 12:56:37 2010 Subject: [Histonet] IgG4 Message-ID: <61135F0455D33347B5AAE209B903A30433DEB713@EXCHVS2.medctr.ad.wfubmc.edu> Hello all, I am looking for a lab that offers this antibody on ffpe tissues. One of my Pathologists is interested in it and has a surgical case she has been asked to stain for IgG4. Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 From MadaryJ <@t> MedImmune.com Mon Mar 1 13:02:01 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Mar 1 13:02:08 2010 Subject: [Histonet] RE: Histonet Digest, Vol 76, Issue 1 In-Reply-To: References: Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1301459274@MD1EV002.medimmune.com> I recall may years ago using the alcohol burner to heat my forceps and I was startled when the ice I had my blox on broke away from the pan and made a loud sound in an otherwise very quiet lab. I knocked over the alcohol burner and started a major lab fire. I used the extinguisher and all was fine but it took a while to get it all out. The biggest fear was the waste containers(good reason to recycle. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, March 01, 2010 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Fire in the lab (Cheryl) 2. Fw: [Histonet] frozen sections of cartilage (Kim Merriam) 3. Pathologist Assistant Jobs (Alisha Dynan) 4. RELIA Histology Careers Bulletin 03/01/10 Are you into Facebook? MySpace? Twitter? or Linkedin? (Pam Barker) 5. CD31 in rats (Annette Featherstone) ---------------------------------------------------------------------- Message: 1 Date: Sun, 28 Feb 2010 17:14:17 -0800 (PST) From: Cheryl Subject: [Histonet] Fire in the lab To: histonet@lists.utsouthwestern.edu Message-ID: <595933.44936.qm@web50901.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Amazing that your tech had the presence of mind to do ALL of those things! ? Used to work with a couple of old school techs back in the days when folks smoked in the lab.? One tech would embed with a xylene soaked rag to wipe the plate and a cigarette hanging out of her mouth.? She also used an open bunsen burner to keep her forceps hot....cannot tell you how many times she set fire to that rag...used her coffee to put it out most times... ? As Louise says--in another time and dimension. ? Cheryl --- On Sun, 2/28/10, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 39 To: histonet@lists.utsouthwestern.edu Date: Sunday, February 28, 2010, 10:01 AM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. Re: Fire in the lab (louise renton) ???2. RE: SPAM-LOW:? [Histonet] ruo antibodies (Patsy Ruegg) ???3. Fire in the lab (Jeffrey Silverman) ???4. RELIA Histology Careers Bulletin 2/28/10 Are you into ? ? ? Facebook? MySpace? Twitter? or Linkedin? (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Sat, 27 Feb 2010 20:51:00 +0200 From: louise renton Subject: Re: [Histonet] Fire in the lab To: Histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 hey, I once set my hair on fire in the lab...singed off my eyebrows and burnt my nostril hair. It took several days for the burnt hair smell to get out of my nose!!. This too was in a time long long ago in another time dimension On Sat, Feb 27, 2010 at 12:51 AM, Joe Nocito wrote: > Once upon a time in a far away land, we used to boil our embedding molds in > boiling soapy water, over an open Bunsen burner, followed by an alcohol > rinse then air dry. One time the fire alarm was activated and we had to > evacuate the hospital. We were out there quit awhile. When we received the > all clear to go back into the hospital, I was the first one back in the lab > and the fire department was there, looking into our pot that had boiled out > and was smoking up the lab. This wasn't the cause of the first alarm, but it > did set off the second. > > Joe > ----- Original Message ----- From: "CHRISTIE GOWAN" > > > To: > Sent: Friday, February 26, 2010 8:20 AM > > Subject: [Histonet] Fire in the lab > > > > > >? Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old paraffin > pot. One of my techs came in on Sunday to embed some tissues, went into the > processor room and smelled something burning. He noticed our old paraffin > pot had charred looking labels on the outside so he went over, opened the > lid and poof!!! the pot went up in flames. The thermostat had gone haywire > and heated the paraffin to flash point. Opening the lid gave it the oxygen > it needed to ignite. He triggered the alarm, made the appropriate call and > then put it out with an extinguisher. Of course it kept re-igniting because > he could not get behind it to pull the plug. The fire dept finally was able > to get it pulled out and unplugged. Needless to say the tech was shaken and > the room was a mess. I applaud his courage and am not sure I would have done > the same. There was enough xylene and alcohol on the 4 processors to cause > quite an explosion but everything else was in a flammable cabinet. I was > wondering if this type of thing had ever happened to anyone else?? Needless > to say, we have de-comissioned all old paraffin pots and will order only > those with over temp safety features. I guess I just wanted to remind > everyone that fires can happen in the lab and do probably more often than we > hear about. This was the first time for me and I have been in this business > for over 20 years. Take care and be safe. > > Christie Gowan HT (ASCP) >? ? ? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 2 Date: Sat, 27 Feb 2010 12:47:01 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW:? [Histonet] ruo antibodies To: "'Vickroy, Jim'" , ??? Message-ID: <5E6E0AB0ED28430797B6300065C77E6D@prueggihctechlt> Content-Type: text/plain;??? charset="us-ascii" Yea we were just looking at this yesterday.? There is no question on the CAP check list about using RUO's anymore, but it is still in the discussion section with ASR's and it is stated as you mentioned to include a disclaimer and statement that you did try to find an IVD or ASR antibody.? Our question was about billing the patient for RUO use.? CAP does not really address billing issues like that, but CMS does as far as we could tell, and we have been told that if you bill for RUO's CMS could come back and deny payment of the claim and perhaps all bills from your lab. We were wondering if anyone is billing patients for RUO antibodies, but of course if you are you may not want to say so to bring attention to yourself. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Friday, February 26, 2010 10:03 AM To: Histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] ruo antibodies Our new CAP checklist does not mention the use of RUO antibodies anymore. This was under the question using ASR antibodies in the past.???I believe the requirement was that if we wanted to use an RUO antibody we had to have a disclaimer similar to the ASR disclaimer but we also had to have a statement stating that we had searched for an IVD or ASR antibody.? Does anyone know if this is still the practice or am I missing something????I do know of course than when using either an ASR or RUO antibody we have to establish and verify the performance.???Any thoughts about the RUO(s)? James Vickroy BS, HT(ASCP) Surgical? and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sat, 27 Feb 2010 12:30:35 -0800 (PST) From: Jeffrey Silverman Subject: [Histonet] Fire in the lab To: histonet@lists.utsouthwestern.edu Message-ID: <442926.94178.qm@web111115.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Here's mine. Had a per diem tech who?didn't like the electric forceps warmer.? She worked over Saturdays alone and brought in an alcohol lamp to heat her forceps and then knocked it over spilling flaming alcohol all over the thermal and dispensing consoles. Then, even worse, she poured water all over both plugged- in electic appliances. We got away with just melted facade?face plates on both instruments. ? Jeff Silverman ------------------------------ Message: 4 Date: Sat, 27 Feb 2010 22:12:50 -0500 From: "Pam Barker" Subject: [Histonet] RELIA Histology Careers Bulletin 2/28/10 Are you ??? into??? Facebook? MySpace? Twitter? or Linkedin? To: "'Histonet'" Message-ID: Content-Type: text/plain;??? charset="us-ascii" Hi Histonetters!! Facebook, Myspace, Linkedin, Twitter;? Have you joined the Social Networking Craze? If so then you know how much fun it can be reconnecting with old friends, making new ones.? If you do have an account on one of these networks I would love to connect with you.? Please shoot me back an e-mail with your id on the site or an invitation to connect/friend/follow and I will respond right away. If that was like a foreign language to you and you would like some help using any of these sites please let me know.? I would be happy to help you join in. I also wanted to tell you about my current job openings.? All of these jobs are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance.? Need help with your resume or following up after the interview or negotiating salary?? Give me a call or shoot me an e-mail.? I would be happy to help out.? This is a complimentary service of RELIA of course. Here is a list of my current open positions HISTOLOGY/PATHOLOGY? MANAGEMENT NY- Orange/Rockland County Histology Supervisor MA - Cape Cod Pathology Supervisor OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA - Central CA - Pathology Supervisor HISTOTECHS FL-Miami Histotechnologist needed for growing private lab GA-Grossing Histotechnologist Night Shift Great Shift diff MA-Boston - Immunohistochemistry Specialist MA-Boston - Histotechs day and evening shift NY-Orange/Rockland County Brand New Lab NYS license or Elig 2nd shift. CA-Los Angeles Histotechnologist afternoon shift PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC - Charlotte PA grad from NAACLES program required FL- Miami Growing private lab MARKETING AND SALES Marketing Rep - Histo/Cyto Products Pacific NW Region CYTOLOGY PA-Pittsburgh Cytology Supervisor If you or any of your friends would like more information on any of the positions listed or help with a job search in another area please contact me.? I would be more than happy to assist you.? You can reach me at 866-607-3542 or? relia1@earthlink.net Remember it never hurts to look. Hope to hear from you soon. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? ? (407)353-5070 FAX:? ? (407)678-2788 Toll Free: (866)607-3542 e-mail:? relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 39 **************************************** ------------------------------ Message: 2 Date: Mon, 1 Mar 2010 04:46:09 -0800 (PST) From: Kim Merriam Subject: Fw: [Histonet] frozen sections of cartilage To: Histonet Message-ID: <836501.4247.qm@web50303.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Guys, I never received a response and I was?hoping that someone would have some tips.? We?are embedding and sectioning rat and mouse knee cartilage and I?am having trouble keeping it on the slides.? Even when I do my IHC by hand, the sections are still?lifting off.? Any suggestions? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ----- Forwarded Message ---- From: Kim Merriam To: Histonet Sent: Mon, February 22, 2010 2:37:58 PM Subject: [Histonet] frozen sections of cartilage Hi Everyone, Any tips for keeping frozen sections of cartilage from falling off the slides?during?IHC staining?? We should not have to do HIER, but they still fall of the slides quite easily. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: 1 Mar 2010 10:11:11 -0500 From: Alisha Dynan Subject: [Histonet] Pathologist Assistant Jobs To: histonet@lists.utsouthwestern.edu Message-ID: <1496304024.1267456271654.JavaMail.cfservice@webserver53> Content-Type: text/plain; charset="utf-8" Dear Histonet Subscribers, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Laboratory Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory positions across the country. Our clients typically assist with relocation expenses. One particular client I am working with is one of the top hospital systems in the country. This hospital in Tennessee is looking for a Pathologist Assistant for their Pathology Department. My client is looking for someone who is PA(ASCP) certified and has at least 5 years experience as a pathologist assistant. This position has a very competitive base salary, great benefits, and an unparalleled retirement package. My client will also assist with relocation expenses. We are also working on a number of other opportunities. See the titles below and let me know if you would like more details! Other Current Opportunities: NV - Pathologist's Assistant 2nd shift TN - Pathologist's Assistant 1st shift (must have Master's) Midwest - Pathologist's Assistant 1st shift Southern CA - Pathologist Assistant Supervisor If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Laboratory positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 4 Date: Mon, 1 Mar 2010 10:28:38 -0500 From: "Pam Barker" Subject: [Histonet] RELIA Histology Careers Bulletin 03/01/10 Are you into Facebook? MySpace? Twitter? or Linkedin? To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters!! Facebook, Myspace, Linkedin, Twitter; Have you joined the Social Networking Craze? If so then you know how much fun it can be reconnecting with old friends, making new ones. If you do have an account on one of these networks I would love to connect with you. Please shoot me back an e-mail with your id on the site or an invitation to connect/friend/follow and I will respond right away. If that was like a foreign language to you and you would like some help using any of these sites please let me know. I would be happy to help you join in. I also wanted to tell you about my current job openings. All of these jobs are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance. Need help with your resume or following up after the interview or negotiating salary? Give me a call or shoot me an e-mail. I would be happy to help out. This is a complimentary service of RELIA of course. Here is a list of my current open positions HISTOLOGY/PATHOLOGY MANAGEMENT MA-Cape Cod Pathology Supervisor OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA-Central CA-Pathology Supervisor HISTOTECHS FL-Miami Histotechnologist needed for growing private lab GA-Grossing Histotechnologist Night Shift Great Shift diff MA-Boston - Immunohistochemistry Specialist MA-Boston - Histotechs day and evening shift NY-Orange/Rockland County Brand New Lab NYS license or Elig 2nd shift. CA-Los Angeles Histotechnologist afternoon shift PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC - Charlotte PA grad from NAACLES program required FL- Miami Growing private lab MARKETING AND SALES Marketing Rep - Histo/Cyto Products Pacific NW Region CYTOLOGY PA-Pittsburgh Cytology Supervisor If you or any of your friends would like more information on any of the positions listed or help with a job search in another area please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember it never hurts to look... Hope to hear from you soon. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 5 Date: Mon, 1 Mar 2010 10:44:51 -0500 From: "Annette Featherstone" Subject: [Histonet] CD31 in rats To: Message-ID: <000001cab956$2215a3d0$6640eb70$@edu> Content-Type: text/plain; charset="us-ascii" Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 1 *************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From LSebree <@t> uwhealth.org Mon Mar 1 13:10:34 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Mar 1 13:11:28 2010 Subject: [Histonet] Herpes Simplex Virus Types 1 & 2 for IHC Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF7BD@UWHC-MAIL01.uwhis.hosp.wisc.edu> Good afternoon, Does anyone have a "nice" HSV I & II concentrate antibody for IHC on FFPE that they use. Biocare recently discontinued their concentrate and we don't do enough of these to warrant buying a predilute. We'd like a cocktail of the 2 types. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From Kim.Donadio <@t> bhcpns.org Mon Mar 1 13:39:54 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Mon Mar 1 13:40:17 2010 Subject: [Histonet] Job opening Message-ID: Hi Histo World, We have a Histologist position available. Seeking someone self motivated, personable and team oriented! Anyone interested please email me. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From joseph.tamasi <@t> bms.com Mon Mar 1 14:00:26 2010 From: joseph.tamasi <@t> bms.com (Tamasi, Joseph) Date: Mon Mar 1 14:00:37 2010 Subject: [Histonet] Ab for Cartilage Message-ID: <845938D0FDD6B54BAD926F60FF10A6A803E12A2DBF@ushpwbmsmmp009.one.ads.bms.com> Dear Histonetters, One of my colleagues is interested in demonstrating a marker for terminal chondrocyte differentiation in rat bone. It would be very helpful for her to know what antibodies others have used successfully for IHC. Thank you in advance for your responses. Joe Tamasi Senior Research Scientist Bristol-Myers Squibb 311 Pennington-Rocky-Hill Road Pennington, NJ 08534 609-818-3288 joseph.tamasi@bms.com ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From LSebree <@t> uwhealth.org Mon Mar 1 14:24:20 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Mar 1 14:25:14 2010 Subject: [Histonet] HSV I & II Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF7BF@UWHC-MAIL01.uwhis.hosp.wisc.edu> Thanks to all who responded to my inquiry about HSV I&II however I'd like to find it in a cocktail. I have since learned that manufacturers can no longer premix ASR antibodies to sell . I guess I'll have to find out if are docs need both or can make do with one. Thanks again, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From LINDA.MARGRAF <@t> childrens.com Mon Mar 1 16:14:15 2010 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Mon Mar 1 16:14:34 2010 Subject: [Histonet] inquiry about Arthur Bolles Lee Message-ID: <4B8BE7D7.F783.00DA.0@childrens.com> Dear Histonetters I got this request which I presume was intended for the Histonet list. Does anyone have information on this gentleman I can pass along? Dear Linda, I would appreciate it you could seek or make an inquiry for me. I am looking for an obituary and other biographical information about the histotechnologist Arthur Bolles Lee (1849 - 1927). Sincerely, Frederick H. Kasten Johnson City, TN Thanks... Linda M Histonet administrator Please consider the environment before printing this e-mail

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From greenjumpyone <@t> hotmail.com Mon Mar 1 19:15:23 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Mon Mar 1 19:15:26 2010 Subject: [Histonet] Microwave processing and IHCs In-Reply-To: References: Message-ID: Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/ From wdesalvo.cac <@t> hotmail.com Mon Mar 1 20:23:00 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Mon Mar 1 20:23:05 2010 Subject: [Histonet] Microwave processing and IHCs In-Reply-To: References: , Message-ID: I have used the Sakura Xpress for 5+ years and have had great success w/ all staining, w/ little to no changes. The Xpress does not use Xylene and that was a change from our conventional processing and reagents, so we did have to collect new control tissues. I say that anytime you change the process, you MUST collect new control tissue and you will ned to document. When CAP comes to visit and they see a MW processor, you will need to show them your validation and control tissue documentation. If you have the possibility of using both conventional or Microwave processd tissue, then make a modified sausage block to include both types of processing protocols, so that work can flow through the lab smoothly. William DeSalvo, B.S., HTL(ASCP) > From: greenjumpyone@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 1 Mar 2010 17:15:23 -0800 > Subject: [Histonet] Microwave processing and IHCs > > > Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? > > We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. > > thanks for any help! > > Michelle > > _________________________________________________________________ > Hotmail: Free, trusted and rich email service. > http://clk.atdmt.com/GBL/go/201469228/direct/01/_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft?s powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/ From tifei <@t> foxmail.com Mon Mar 1 21:32:29 2010 From: tifei <@t> foxmail.com (TF) Date: Mon Mar 1 21:32:38 2010 Subject: [Histonet] CD31 in rats References: <000001cab956$2215a3d0$6640eb70$@edu>, <63EC5E7C7346EB4BA09D6DC5651E061BEA5E1E@ent-pr-xch-02.na01.crl.com> Message-ID: <201003021132284371600@foxmail.com> SGksIHdlIHVzZSBBYmNhbSBtb3VzZS1hbnRpIENEMzENCg0KYWxzbyBhY2V0b25lIGZpeGVkIGZy b3plbiBzZWN0aW9ucy4NCnByZXR0eSB3ZWxsLg0KDQoNCjIwMTAtMDMtMDIgDQoNCg0KDQpURiAN Cg0KDQoNCreivP7Iy6O6IEhlcm5hbmRleiwgQW5uYSANCreiy83Ksbzko7ogMjAxMC0wMy0wMiAg MDk6NTA6MzggDQrK1bz+yMujuiBBbm5ldHRlIEZlYXRoZXJzdG9uZTsgaGlzdG9uZXRAbGlzdHMu dXRzb3V0aHdlc3Rlcm4uZWR1IA0Ks63LzaO6IA0K1vfM4qO6IFJFOiBbSGlzdG9uZXRdIENEMzEg aW4gcmF0cyANCiANCkhlbGxvIEFubmV0dGUsDQpXZSB1c2UgUHVyaWZpZWQgTW91c2UgYW50aS1S YXQgQ0QzMSBmcm9tIEJEIEJpb3NjaWVuY2VzIChDYXRhbG9nIw0KNTU1MDI1KS4gIFdlIHVzZSBv biBmcm96ZW4gc2VjdGlvbnMgZml4ZWQgd2l0aCBhY2V0b25lIGZvciAxMCBtaW51dGVzDQp3aXRo IHRoZSBmb2xsb3dpbmcgc3RlcHM6DQoxWCBNb3JwaG9zYXZlIChWZW50YW5hKSAoMTUgbWluKQ0K MC4zJSBIMjAyIHF1ZW5jaChTaWdtYSkgKDIwIG1pbikNCkF2aWRpbiBhbmQgQmlvdGluIGJsb2Nr IChWZWN0b3IpICgxNSBtaW4gZWFjaCkNCkNvdmFuY2UgYmxvY2tpbmcgcmVhZ2VudCAoMjUgbWlu KQ0KTW91c2UgYW50aS1SYXQgQ0QzMSAoMjUgbWluKSBhdCAyLjAgdWcvbUwNCkJpb3RpbnlsYXRl ZCBIb3JzZSBhbnRpLU1vdXNlIElnRyAoVmVjdG9yLUJBLTIwMDEpICgyNSBtaW51dGVzKSBhdCA1 LjANCnVnL21MDQpDb3ZhbmNlIExhYmVsaW5nIFJlYWdlbnQgKDI1IG1pbikNCkNvdmFuY2UgRGFi ICgyIHggNSBtaW4pDQooRWFjaCBzdGVwIGhhcyBQQlMgd2FzaGVzIGluIGJldHdlZW4gYW5kIGlz IHBlcmZvcm1lZCBhdCBSVCkNClRoYW5rcywNCkFubmEgDQotLS0tLU9yaWdpbmFsIE1lc3NhZ2Ut LS0tLQ0KRnJvbTogaGlzdG9uZXQtYm91bmNlc0BsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCltt YWlsdG86aGlzdG9uZXQtYm91bmNlc0BsaXN0cy51dHNvdXRod2VzdGVybi5lZHVdIE9uIEJlaGFs ZiBPZiBBbm5ldHRlDQpGZWF0aGVyc3RvbmUNClNlbnQ6IE1vbmRheSwgTWFyY2ggMDEsIDIwMTAg Nzo0NSBBTQ0KVG86IGhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0KU3ViamVjdDog W0hpc3RvbmV0XSBDRDMxIGluIHJhdHMNCklzIGFueW9uZSB1c2luZyBDRDMxIGFudGlib2R5IGlu IHJhdHMsIGlmIHNvLCB3aGF0IHZlbmRvciBhbmQgd2hhdA0KcHJvdG9jb2wuDQp0aGFua3MNCg0K QW5uZXR0ZSBGZWF0aGVyc3RvbmUNCg0KX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX18NCkhpc3RvbmV0IG1haWxpbmcgbGlzdA0KSGlzdG9uZXRAbGlzdHMudXRz b3V0aHdlc3Rlcm4uZWR1DQpodHRwOi8vbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1L21haWxtYW4v bGlzdGluZm8vaGlzdG9uZXQNCl9fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fDQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3 ZXN0ZXJuLmVkdQ0KaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3Rp bmZvL2hpc3RvbmV0DQo= From W.E.J.Hoekert <@t> olvg.nl Tue Mar 2 03:37:02 2010 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Tue Mar 2 03:37:47 2010 Subject: [Histonet] HSV I & II References: <8C023B4AB999614BA4791BAEB26E27381BF7BF@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <1190CB05C44B13409483514729C2FC360C0A9E@PAIT42.olvg.nl> Too bad that all these people did not respond to the entire group. We are using the one from Immunologic (polyclonal, ILP4114-C1). Is this one also not available anymore? Willem Hoekert OLVG, Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Sebree Linda A Verzonden: ma 1-3-2010 21:24 Aan: Histonet Onderwerp: [Histonet] HSV I & II Thanks to all who responded to my inquiry about HSV I&II however I'd like to find it in a cocktail. I have since learned that manufacturers can no longer premix ASR antibodies to sell . I guess I'll have to find out if are docs need both or can make do with one. Thanks again, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From mpence <@t> grhs.net Tue Mar 2 08:03:59 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Mar 2 08:04:03 2010 Subject: [Histonet] Microwave processing and IHCs In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D79@is-e2k3.grhs.net> Your control tissue needs to be processed the same as your patient samples. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Monday, March 01, 2010 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing and IHCs Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 2 08:03:58 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 2 08:04:17 2010 Subject: [Histonet] Microwave processing and IHCs In-Reply-To: Message-ID: <458340.86147.qm@web65704.mail.ac4.yahoo.com> Michelle: The key in MW tissue processing is to accelerate the process and it is very likely that the quality of the tissues will be the same as if processed in a more "traditional" way, at least that is the claim and the objective. Having said that this does not mean that you don't have to demonstrate it with a validation process. You should always have controls processed in the very same way as the patients' tissues so in theory you have to obtain a new set of controls processed with the your MW tissue processor. The complication develops if you have also a "conventional" tissue processor and then you will need two sets of controls. Ideally all your cases should be processed with a single method. Ren? J. --- On Mon, 3/1/10, Green JumpyOne wrote: From: Green JumpyOne Subject: [Histonet] Microwave processing and IHCs To: histonet@lists.utsouthwestern.edu Date: Monday, March 1, 2010, 8:15 PM Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed.? If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle ??? ???????? ?????? ??? ? _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Mar 2 08:15:11 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Mar 2 08:19:28 2010 Subject: [Histonet] Microwave processing and IHCs In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3D79@is-e2k3.grhs.net> References: , <661949901A768E4F9CC16D8AF8F2838C017A3D79@is-e2k3.grhs.net> Message-ID: In my experience I have not seen any change in the MW tissue versus the routine processing. What we have tried to do since we have so many different antibodies and control tissues is create mini sausage blocks with one piece of MW tissue and one piece of non MW tissue. Because we were not always sure what tissue were microwaved and what tissues were not. For example one small piece of tonsil microwaved and one non microwaved embedded in the same block. That way no matter what your patient tissue sample has gone through you have all the bases covered. It takes a long time to set up but will save you time in the end. If you run your tissues when you validate the microwave you'll have half of the process finished. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpence@grhs.net] Sent: Tuesday, March 02, 2010 9:03 AM To: Green JumpyOne; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing and IHCs Your control tissue needs to be processed the same as your patient samples. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Monday, March 01, 2010 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing and IHCs Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Tue Mar 2 08:37:49 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Mar 2 08:37:56 2010 Subject: FW: [Histonet] Microwave processing and IHCs Message-ID: <24A4826E8EF0964D86BC5317306F58A54257908A0A@mmc-mail.ad.mhsil.com> See below James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: Vickroy, Jim Sent: Tuesday, March 02, 2010 8:16 AM To: 'Mike Pence' Subject: RE: [Histonet] Microwave processing and IHCs I understand and agree in principle. However let me ask this: If a validation study shows that there is no difference between conventional tissue processing and microwave tissue processing in your IHC protocols then why can't you then use controls that are processed either by conventional or microwave. If we can't then are we using controls that have been decaled for tissues that have to be decaled and so forth. I have always believed if you can validate there is no appreciable difference in methods than we can use the same controls. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, March 02, 2010 8:04 AM To: Green JumpyOne; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing and IHCs Your control tissue needs to be processed the same as your patient samples. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Monday, March 01, 2010 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing and IHCs Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From HornHV <@t> archildrens.org Tue Mar 2 08:49:50 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Mar 2 08:50:19 2010 Subject: [Histonet] inquiry about Arthur Bolles Lee In-Reply-To: <4B8BE7D7.F783.00DA.0@childrens.com> References: <4B8BE7D7.F783.00DA.0@childrens.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83738@EMAIL.archildrens.org> If you plug his name into google several links appear.. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Monday, March 01, 2010 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inquiry about Arthur Bolles Lee Dear Histonetters I got this request which I presume was intended for the Histonet list. Does anyone have information on this gentleman I can pass along? Dear Linda, I would appreciate it you could seek or make an inquiry for me. I am looking for an obituary and other biographical information about the histotechnologist Arthur Bolles Lee (1849 - 1927). Sincerely, Frederick H. Kasten Johnson City, TN Thanks... Linda M Histonet administrator Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
information that is confidential and privileged. This information is intended only for the use of the
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disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
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please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
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applicable privileges related to this information.

_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From chak_bou <@t> yahoo.com Tue Mar 2 09:06:20 2010 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Tue Mar 2 09:06:23 2010 Subject: [Histonet] IL-17A Message-ID: <417367.27024.qm@web58107.mail.re3.yahoo.com> Is anyone using IL17A in mice? where do you buy it?Dilution? where do you buy the secondary? dilution? The protocol ? Thank you so much. Chakib From srodriguez <@t> phenopath.com Tue Mar 2 09:35:13 2010 From: srodriguez <@t> phenopath.com (Stephanie Rodriguez) Date: Tue Mar 2 09:35:34 2010 Subject: [Histonet] Re: Histonet Digest, Vol 76, Issue 2 Message-ID: Hi Martha, We offer this antibody. Visit our website: www.phenopath.com or call (206) 374-9000 and ask for Client Services for information on specimen submission. Thanks, Stephanie Rodriguez, HTL(ASCP), QIHC IHC Tech III/Clinical Molecular Tech Phenopath Laboratories Seattle, WA On 3/2/10 7:03 AM, "histonet-request@lists.utsouthwestern.edu" wrote: > > Message: 4 > Date: Mon, 1 Mar 2010 13:56:17 -0500 > From: "Martha Ward" > Subject: [Histonet] IgG4 > To: > Message-ID: > <61135F0455D33347B5AAE209B903A30433DEB713@EXCHVS2.medctr.ad.wfubmc.edu> > > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > > I am looking for a lab that offers this antibody on ffpe tissues. One > of my Pathologists is interested in it and has a surgical case she has > been asked to stain for IgG4. Thanks in advance for your help! > > Martha Ward, MT (ASCP) QIHC > Assistant Manager, Molecular Diagnostics Lab > Wake Forest University Baptist Medical Center > 336-716-2104 This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From AWeiss <@t> shorememorial.org Tue Mar 2 09:43:05 2010 From: AWeiss <@t> shorememorial.org (AWeiss@shorememorial.org) Date: Tue Mar 2 09:44:52 2010 Subject: [Histonet] Billing for H&E stains Message-ID: Good Morning Anyone out there doing H&E on cytology slides? Currently I am trying to do H&E stains on our FNA's, is there a separate charge for billing the H&E per slide? Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. From DKBoyd <@t> chs.net Tue Mar 2 09:56:39 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Mar 2 09:56:47 2010 Subject: [Histonet] Billing for H&E stains In-Reply-To: Message-ID: I've never done a H&E on cytology just a Pap stain and Diff Quik. The stain charge, however is determined by the preparation technique (a smear or cytospin concentration preparation). The cytospin prep (Non Gyn) is 88108: the smear (NonGyn) is 88104. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net AWeiss@shorememorial.org Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2010 10:45 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Billing for H&E stains Good Morning Anyone out there doing H&E on cytology slides? Currently I am trying to do H&E stains on our FNA's, is there a separate charge for billing the H&E per slide? Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From BUSTAMANTES <@t> uthscsa.edu Tue Mar 2 10:01:19 2010 From: BUSTAMANTES <@t> uthscsa.edu (Bustamante, Sonja A) Date: Tue Mar 2 10:01:24 2010 Subject: [Histonet] Sanderson's Bone Stain Message-ID: Hello anyone out there, A couple of years ago, I ordered Sanderson's Rapid Bone Stain from Surgipath. Since they have gotten bought out, they no longer carry this stain. Does anyone know where I can order it? Thanks From Rcartun <@t> harthosp.org Tue Mar 2 10:33:24 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Mar 2 10:33:35 2010 Subject: [Histonet] Ultram fixative Message-ID: <4B8CF783.7400.0077.1@harthosp.org> We have a clinic that is fixing tissue in "Ultram". I have never heard of it. Can someone educate me about "Ultram"? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From ratliffjack <@t> hotmail.com Tue Mar 2 10:42:36 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Mar 2 10:42:41 2010 Subject: [Histonet] Sanderson's Bone Stain In-Reply-To: References: Message-ID: This stain is now available via Dorn and Hart Microedge - www.dornandhart.com Jack > From: BUSTAMANTES@uthscsa.edu > To: histonet@lists.utsouthwestern.edu > Date: Tue, 2 Mar 2010 10:01:19 -0600 > Subject: [Histonet] Sanderson's Bone Stain > > Hello anyone out there, > A couple of years ago, I ordered Sanderson's Rapid Bone Stain from Surgipath. Since they have gotten bought out, they no longer carry this stain. Does anyone know where I can order it? Thanks > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Tue Mar 2 10:44:37 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Mar 2 10:44:43 2010 Subject: [Histonet] RE: Sanderson's Bone Stain In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE2242A4535E5@MERCERMAIL.MercerU.local> Go to www.histosearch.com and to the Histonet archives, there have been sources for this stain discussed recently. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Sonja A Sent: Tuesday, March 02, 2010 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sanderson's Bone Stain Hello anyone out there, A couple of years ago, I ordered Sanderson's Rapid Bone Stain from Surgipath. Since they have gotten bought out, they no longer carry this stain. Does anyone know where I can order it? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Tue Mar 2 10:44:43 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 2 10:44:48 2010 Subject: [Histonet] Alcohol Source? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46EE8@nmdamailsvr.nmda.ad.nmsu.edu> I hate to even ADMIT this and I ought to use a disguise when asking - but is alcohol a wood-based solution? I should know this - I know... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From sbreeden <@t> nmda.nmsu.edu Tue Mar 2 10:47:45 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 2 10:47:49 2010 Subject: [Histonet] Alcohol followup Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46EE9@nmdamailsvr.nmda.ad.nmsu.edu> As I pressed the "SEND" button it came to me like a bolt of lightning that alcohol is GRAIN based. Oops... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From rjbuesa <@t> yahoo.com Tue Mar 2 10:59:18 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 2 10:59:22 2010 Subject: [Histonet] Alcohol followup In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46EE9@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <961455.24211.qm@web65714.mail.ac4.yahoo.com> Neither one nor the other. Ethanol, the alcohol you are referring to, is the product of fermentation on sugars existing in?either fruits (any fruit)?or grains, so it cannot be limited to grains. Methanol is the product of fermentation on cellulose,?specially from wood. Ren? J. ? --- On Tue, 3/2/10, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Alcohol followup To: "histonet" Date: Tuesday, March 2, 2010, 11:47 AM As I pressed the "SEND" button it came to me like a bolt of lightning that alcohol is GRAIN based.? Oops... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From greenjumpyone <@t> hotmail.com Tue Mar 2 11:23:23 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Tue Mar 2 11:23:27 2010 Subject: [Histonet] Workflow suggestions for MW processing In-Reply-To: References: Message-ID: Thanks to all who have taken the time to reply to my question on microwave tissue processing and the resulting effect (or not) on IHC staining. As you have probably guessed, I am in the process of getting our MWs validated and set up for use. We are still using our conventional processors, but I am very excited about bringing the MWs online. That brings up another issue for me: workflow. We are a M-F lab, pathologists are here from 8-5. They will not be changing their schedules to accommodate the new MWs, so I am left with the task of figuring out the most efficient way to manage my staff, the specimens and the processing. Our basic situation: I currently have 3 histotechs (and myself, if needed on the bench). One comes in at 1am, one at 5am and one at 7am. These shifts can change if needed to make us more efficient. Paths expect slides beginning at 8:30am. The MWs are semi-automatic. They require a human to swap out the cassettes into the next reagent and to press the button to continue the processing. Grossing is done between 10am and 5:30pm. We have two MWs, so we can run smalls and large specimens concurrently. Does anyone have a situation similar to ours? Do you have any suggestions on pitfalls to watch out for? Any suggestions on the "best" way to achieve both efficiency and good utilization of my staff? THANKS for any thoughts you might like to share! Michelle _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/ From mdeguzman <@t> lifecell.com Tue Mar 2 11:45:20 2010 From: mdeguzman <@t> lifecell.com (DeGuzman, Maria) Date: Tue Mar 2 11:45:33 2010 Subject: : [Histonet] frozen sections of cartilage In-Reply-To: <1ac3dcba-65ae-4e37-b55a-35f15a0419e2@amwpht01.kci.com> References: <1ac3dcba-65ae-4e37-b55a-35f15a0419e2@amwpht01.kci.com> Message-ID: <5476245379016B4D8212E8BCD11ECFFB77907555@AMWPVEX01.kci.com> Hi Kim, Try probe on plus slides by fisherbrand for frozen samples. We use it for sectioning frozen tendon. Maria V. De Guzman| Histology Technician I Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1482 Email mdeguzman@lifecell.com Mobile 732.688.1386 www.Lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, March 02, 2010 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD31 in rats (Hernandez, Anna) 2. CD 31 on Pig (Reuel Cornelia) 3. Re: CD 31 on Pig (Alexandra Meinl) 4. IgG4 (Martha Ward) 5. RE: Histonet Digest, Vol 76, Issue 1 (Madary, Joseph) 6. Herpes Simplex Virus Types 1 & 2 for IHC (Sebree Linda A) 7. Job opening (Kim.Donadio@bhcpns.org) 8. Ab for Cartilage (Tamasi, Joseph) 9. HSV I & II (Sebree Linda A) 10. inquiry about Arthur Bolles Lee (LINDA MARGRAF) 11. Microwave processing and IHCs (Green JumpyOne) 12. RE: Microwave processing and IHCs (WILLIAM DESALVO) 13. Re: RE: [Histonet] CD31 in rats (TF) 14. RE: HSV I & II (Hoekert, W.E.J.) 15. RE: Microwave processing and IHCs (Mike Pence) 16. Re: Microwave processing and IHCs (Rene J Buesa) 17. RE: Microwave processing and IHCs (McMahon, Loralee A) 18. FW: [Histonet] Microwave processing and IHCs (Vickroy, Jim) 19. RE: inquiry about Arthur Bolles Lee (Horn, Hazel V) ---------------------------------------------------------------------- Message: 1 Date: Mon, 1 Mar 2010 13:01:03 -0500 From: "Hernandez, Anna" Subject: RE: [Histonet] CD31 in rats To: "Annette Featherstone" , Message-ID: <63EC5E7C7346EB4BA09D6DC5651E061BEA5E1E@ent-pr-xch-02.na01.crl.com> Content-Type: text/plain; charset="us-ascii" Hello Annette, We use Purified Mouse anti-Rat CD31 from BD Biosciences (Catalog# 555025). We use on frozen sections fixed with acetone for 10 minutes with the following steps: 1X Morphosave (Ventana) (15 min) 0.3% H202 quench(Sigma) (20 min) Avidin and Biotin block (Vector) (15 min each) Covance blocking reagent (25 min) Mouse anti-Rat CD31 (25 min) at 2.0 ug/mL Biotinylated Horse anti-Mouse IgG (Vector-BA-2001) (25 minutes) at 5.0 ug/mL Covance Labeling Reagent (25 min) Covance Dab (2 x 5 min) (Each step has PBS washes in between and is performed at RT) Thanks, Anna -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone Sent: Monday, March 01, 2010 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31 in rats Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 01 Mar 2010 12:10:13 -0600 From: "Reuel Cornelia" Subject: [Histonet] CD 31 on Pig To: Message-ID: <4B8BAEA5020000C5000719EC@mail.TSRH.ORG> Content-Type: text/plain; charset=US-ASCII Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank you. Reuel ------------------------------ Message: 3 Date: Mon, 1 Mar 2010 19:37:31 +0100 From: Alexandra Meinl Subject: Re: [Histonet] CD 31 on Pig To: Reuel Cornelia , af46@buffalo.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 We use the Rabbit anti-PECAM-1 (sc-1506-R, Santa Cruz) 1:100 on paraffin sections (HIER with citrate buffer pH 6.0 required). It works on pig and rat tissue. Alexandra 2010/3/1 Reuel Cornelia > Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank > you. > > Reuel > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.meinl@trauma.lbg.ac.at ------------------------------ Message: 4 Date: Mon, 1 Mar 2010 13:56:17 -0500 From: "Martha Ward" Subject: [Histonet] IgG4 To: Message-ID: <61135F0455D33347B5AAE209B903A30433DEB713@EXCHVS2.medctr.ad.wfubmc.edu> Content-Type: text/plain; charset="us-ascii" Hello all, I am looking for a lab that offers this antibody on ffpe tissues. One of my Pathologists is interested in it and has a surgical case she has been asked to stain for IgG4. Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ------------------------------ Message: 5 Date: Mon, 1 Mar 2010 14:02:01 -0500 From: "Madary, Joseph" Subject: [Histonet] RE: Histonet Digest, Vol 76, Issue 1 To: Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1301459274@MD1EV002.medimmune.com> Content-Type: text/plain; charset="iso-8859-1" I recall may years ago using the alcohol burner to heat my forceps and I was startled when the ice I had my blox on broke away from the pan and made a loud sound in an otherwise very quiet lab. I knocked over the alcohol burner and started a major lab fire. I used the extinguisher and all was fine but it took a while to get it all out. The biggest fear was the waste containers(good reason to recycle. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, March 01, 2010 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Fire in the lab (Cheryl) 2. Fw: [Histonet] frozen sections of cartilage (Kim Merriam) 3. Pathologist Assistant Jobs (Alisha Dynan) 4. RELIA Histology Careers Bulletin 03/01/10 Are you into Facebook? MySpace? Twitter? or Linkedin? (Pam Barker) 5. CD31 in rats (Annette Featherstone) ---------------------------------------------------------------------- Message: 1 Date: Sun, 28 Feb 2010 17:14:17 -0800 (PST) From: Cheryl Subject: [Histonet] Fire in the lab To: histonet@lists.utsouthwestern.edu Message-ID: <595933.44936.qm@web50901.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Amazing that your tech had the presence of mind to do ALL of those things! ? Used to work with a couple of old school techs back in the days when folks smoked in the lab.? One tech would embed with a xylene soaked rag to wipe the plate and a cigarette hanging out of her mouth.? She also used an open bunsen burner to keep her forceps hot....cannot tell you how many times she set fire to that rag...used her coffee to put it out most times... ? As Louise says--in another time and dimension. ? Cheryl --- On Sun, 2/28/10, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 39 To: histonet@lists.utsouthwestern.edu Date: Sunday, February 28, 2010, 10:01 AM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. Re: Fire in the lab (louise renton) ???2. RE: SPAM-LOW:? [Histonet] ruo antibodies (Patsy Ruegg) ???3. Fire in the lab (Jeffrey Silverman) ???4. RELIA Histology Careers Bulletin 2/28/10 Are you into ? ? ? Facebook? MySpace? Twitter? or Linkedin? (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Sat, 27 Feb 2010 20:51:00 +0200 From: louise renton Subject: Re: [Histonet] Fire in the lab To: Histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 hey, I once set my hair on fire in the lab...singed off my eyebrows and burnt my nostril hair. It took several days for the burnt hair smell to get out of my nose!!. This too was in a time long long ago in another time dimension On Sat, Feb 27, 2010 at 12:51 AM, Joe Nocito wrote: > Once upon a time in a far away land, we used to boil our embedding molds in > boiling soapy water, over an open Bunsen burner, followed by an alcohol > rinse then air dry. One time the fire alarm was activated and we had to > evacuate the hospital. We were out there quit awhile. When we received the > all clear to go back into the hospital, I was the first one back in the lab > and the fire department was there, looking into our pot that had boiled out > and was smoking up the lab. This wasn't the cause of the first alarm, but it > did set off the second. > > Joe > ----- Original Message ----- From: "CHRISTIE GOWAN" > > > To: > Sent: Friday, February 26, 2010 8:20 AM > > Subject: [Histonet] Fire in the lab > > > > > >? Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old paraffin > pot. One of my techs came in on Sunday to embed some tissues, went into the > processor room and smelled something burning. He noticed our old paraffin > pot had charred looking labels on the outside so he went over, opened the > lid and poof!!! the pot went up in flames. The thermostat had gone haywire > and heated the paraffin to flash point. Opening the lid gave it the oxygen > it needed to ignite. He triggered the alarm, made the appropriate call and > then put it out with an extinguisher. Of course it kept re-igniting because > he could not get behind it to pull the plug. The fire dept finally was able > to get it pulled out and unplugged. Needless to say the tech was shaken and > the room was a mess. I applaud his courage and am not sure I would have done > the same. There was enough xylene and alcohol on the 4 processors to cause > quite an explosion but everything else was in a flammable cabinet. I was > wondering if this type of thing had ever happened to anyone else?? Needless > to say, we have de-comissioned all old paraffin pots and will order only > those with over temp safety features. I guess I just wanted to remind > everyone that fires can happen in the lab and do probably more often than we > hear about. This was the first time for me and I have been in this business > for over 20 years. Take care and be safe. > > Christie Gowan HT (ASCP) >? ? ? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 2 Date: Sat, 27 Feb 2010 12:47:01 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW:? [Histonet] ruo antibodies To: "'Vickroy, Jim'" , ??? Message-ID: <5E6E0AB0ED28430797B6300065C77E6D@prueggihctechlt> Content-Type: text/plain;??? charset="us-ascii" Yea we were just looking at this yesterday.? There is no question on the CAP check list about using RUO's anymore, but it is still in the discussion section with ASR's and it is stated as you mentioned to include a disclaimer and statement that you did try to find an IVD or ASR antibody.? Our question was about billing the patient for RUO use.? CAP does not really address billing issues like that, but CMS does as far as we could tell, and we have been told that if you bill for RUO's CMS could come back and deny payment of the claim and perhaps all bills from your lab. We were wondering if anyone is billing patients for RUO antibodies, but of course if you are you may not want to say so to bring attention to yourself. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Friday, February 26, 2010 10:03 AM To: Histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] ruo antibodies Our new CAP checklist does not mention the use of RUO antibodies anymore. This was under the question using ASR antibodies in the past.???I believe the requirement was that if we wanted to use an RUO antibody we had to have a disclaimer similar to the ASR disclaimer but we also had to have a statement stating that we had searched for an IVD or ASR antibody.? Does anyone know if this is still the practice or am I missing something????I do know of course than when using either an ASR or RUO antibody we have to establish and verify the performance.???Any thoughts about the RUO(s)? James Vickroy BS, HT(ASCP) Surgical? and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sat, 27 Feb 2010 12:30:35 -0800 (PST) From: Jeffrey Silverman Subject: [Histonet] Fire in the lab To: histonet@lists.utsouthwestern.edu Message-ID: <442926.94178.qm@web111115.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Here's mine. Had a per diem tech who?didn't like the electric forceps warmer.? She worked over Saturdays alone and brought in an alcohol lamp to heat her forceps and then knocked it over spilling flaming alcohol all over the thermal and dispensing consoles. Then, even worse, she poured water all over both plugged- in electic appliances. We got away with just melted facade?face plates on both instruments. ? Jeff Silverman ------------------------------ Message: 4 Date: Sat, 27 Feb 2010 22:12:50 -0500 From: "Pam Barker" Subject: [Histonet] RELIA Histology Careers Bulletin 2/28/10 Are you ??? into??? Facebook? MySpace? Twitter? or Linkedin? To: "'Histonet'" Message-ID: Content-Type: text/plain;??? charset="us-ascii" Hi Histonetters!! Facebook, Myspace, Linkedin, Twitter;? Have you joined the Social Networking Craze? If so then you know how much fun it can be reconnecting with old friends, making new ones.? If you do have an account on one of these networks I would love to connect with you.? Please shoot me back an e-mail with your id on the site or an invitation to connect/friend/follow and I will respond right away. If that was like a foreign language to you and you would like some help using any of these sites please let me know.? I would be happy to help you join in. I also wanted to tell you about my current job openings.? All of these jobs are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance.? Need help with your resume or following up after the interview or negotiating salary?? Give me a call or shoot me an e-mail.? I would be happy to help out.? This is a complimentary service of RELIA of course. Here is a list of my current open positions HISTOLOGY/PATHOLOGY? MANAGEMENT NY- Orange/Rockland County Histology Supervisor MA - Cape Cod Pathology Supervisor OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA - Central CA - Pathology Supervisor HISTOTECHS FL-Miami Histotechnologist needed for growing private lab GA-Grossing Histotechnologist Night Shift Great Shift diff MA-Boston - Immunohistochemistry Specialist MA-Boston - Histotechs day and evening shift NY-Orange/Rockland County Brand New Lab NYS license or Elig 2nd shift. CA-Los Angeles Histotechnologist afternoon shift PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC - Charlotte PA grad from NAACLES program required FL- Miami Growing private lab MARKETING AND SALES Marketing Rep - Histo/Cyto Products Pacific NW Region CYTOLOGY PA-Pittsburgh Cytology Supervisor If you or any of your friends would like more information on any of the positions listed or help with a job search in another area please contact me.? I would be more than happy to assist you.? You can reach me at 866-607-3542 or? relia1@earthlink.net Remember it never hurts to look. Hope to hear from you soon. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? ? (407)353-5070 FAX:? ? (407)678-2788 Toll Free: (866)607-3542 e-mail:? relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 39 **************************************** ------------------------------ Message: 2 Date: Mon, 1 Mar 2010 04:46:09 -0800 (PST) From: Kim Merriam Subject: Fw: [Histonet] frozen sections of cartilage To: Histonet Message-ID: <836501.4247.qm@web50303.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Guys, I never received a response and I was?hoping that someone would have some tips.? We?are embedding and sectioning rat and mouse knee cartilage and I?am having trouble keeping it on the slides.? Even when I do my IHC by hand, the sections are still?lifting off.? Any suggestions? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ----- Forwarded Message ---- From: Kim Merriam To: Histonet Sent: Mon, February 22, 2010 2:37:58 PM Subject: [Histonet] frozen sections of cartilage Hi Everyone, Any tips for keeping frozen sections of cartilage from falling off the slides?during?IHC staining?? We should not have to do HIER, but they still fall of the slides quite easily. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: 1 Mar 2010 10:11:11 -0500 From: Alisha Dynan Subject: [Histonet] Pathologist Assistant Jobs To: histonet@lists.utsouthwestern.edu Message-ID: <1496304024.1267456271654.JavaMail.cfservice@webserver53> Content-Type: text/plain; charset="utf-8" Dear Histonet Subscribers, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Laboratory Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory positions across the country. Our clients typically assist with relocation expenses. One particular client I am working with is one of the top hospital systems in the country. This hospital in Tennessee is looking for a Pathologist Assistant for their Pathology Department. My client is looking for someone who is PA(ASCP) certified and has at least 5 years experience as a pathologist assistant. This position has a very competitive base salary, great benefits, and an unparalleled retirement package. My client will also assist with relocation expenses. We are also working on a number of other opportunities. See the titles below and let me know if you would like more details! Other Current Opportunities: NV - Pathologist's Assistant 2nd shift TN - Pathologist's Assistant 1st shift (must have Master's) Midwest - Pathologist's Assistant 1st shift Southern CA - Pathologist Assistant Supervisor If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Laboratory positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 4 Date: Mon, 1 Mar 2010 10:28:38 -0500 From: "Pam Barker" Subject: [Histonet] RELIA Histology Careers Bulletin 03/01/10 Are you into Facebook? MySpace? Twitter? or Linkedin? To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters!! Facebook, Myspace, Linkedin, Twitter; Have you joined the Social Networking Craze? If so then you know how much fun it can be reconnecting with old friends, making new ones. If you do have an account on one of these networks I would love to connect with you. Please shoot me back an e-mail with your id on the site or an invitation to connect/friend/follow and I will respond right away. If that was like a foreign language to you and you would like some help using any of these sites please let me know. I would be happy to help you join in. I also wanted to tell you about my current job openings. All of these jobs are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance. Need help with your resume or following up after the interview or negotiating salary? Give me a call or shoot me an e-mail. I would be happy to help out. This is a complimentary service of RELIA of course. Here is a list of my current open positions HISTOLOGY/PATHOLOGY MANAGEMENT MA-Cape Cod Pathology Supervisor OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA-Central CA-Pathology Supervisor HISTOTECHS FL-Miami Histotechnologist needed for growing private lab GA-Grossing Histotechnologist Night Shift Great Shift diff MA-Boston - Immunohistochemistry Specialist MA-Boston - Histotechs day and evening shift NY-Orange/Rockland County Brand New Lab NYS license or Elig 2nd shift. CA-Los Angeles Histotechnologist afternoon shift PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC - Charlotte PA grad from NAACLES program required FL- Miami Growing private lab MARKETING AND SALES Marketing Rep - Histo/Cyto Products Pacific NW Region CYTOLOGY PA-Pittsburgh Cytology Supervisor If you or any of your friends would like more information on any of the positions listed or help with a job search in another area please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember it never hurts to look... Hope to hear from you soon. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 5 Date: Mon, 1 Mar 2010 10:44:51 -0500 From: "Annette Featherstone" Subject: [Histonet] CD31 in rats To: Message-ID: <000001cab956$2215a3d0$6640eb70$@edu> Content-Type: text/plain; charset="us-ascii" Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 1 *************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ------------------------------ Message: 6 Date: Mon, 1 Mar 2010 13:10:34 -0600 From: "Sebree Linda A" Subject: [Histonet] Herpes Simplex Virus Types 1 & 2 for IHC To: "Histonet" Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF7BD@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Good afternoon, Does anyone have a "nice" HSV I & II concentrate antibody for IHC on FFPE that they use. Biocare recently discontinued their concentrate and we don't do enough of these to warrant buying a predilute. We'd like a cocktail of the 2 types. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ------------------------------ Message: 7 Date: Mon, 1 Mar 2010 13:39:54 -0600 From: Kim.Donadio@bhcpns.org Subject: [Histonet] Job opening To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi Histo World, We have a Histologist position available. Seeking someone self motivated, personable and team oriented! Anyone interested please email me. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 8 Date: Mon, 1 Mar 2010 15:00:26 -0500 From: "Tamasi, Joseph" Subject: [Histonet] Ab for Cartilage To: "histonet@lists.utsouthwestern.edu" Message-ID: <845938D0FDD6B54BAD926F60FF10A6A803E12A2DBF@ushpwbmsmmp009.one.ads.bms.com> Content-Type: text/plain; charset="us-ascii" Dear Histonetters, One of my colleagues is interested in demonstrating a marker for terminal chondrocyte differentiation in rat bone. It would be very helpful for her to know what antibodies others have used successfully for IHC. Thank you in advance for your responses. Joe Tamasi Senior Research Scientist Bristol-Myers Squibb 311 Pennington-Rocky-Hill Road Pennington, NJ 08534 609-818-3288 joseph.tamasi@bms.com ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ------------------------------ Message: 9 Date: Mon, 1 Mar 2010 14:24:20 -0600 From: "Sebree Linda A" Subject: [Histonet] HSV I & II To: "Histonet" Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF7BF@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Thanks to all who responded to my inquiry about HSV I&II however I'd like to find it in a cocktail. I have since learned that manufacturers can no longer premix ASR antibodies to sell . I guess I'll have to find out if are docs need both or can make do with one. Thanks again, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ------------------------------ Message: 10 Date: Mon, 01 Mar 2010 16:14:15 -0600 From: "LINDA MARGRAF" Subject: [Histonet] inquiry about Arthur Bolles Lee To: Message-ID: <4B8BE7D7.F783.00DA.0@childrens.com> Content-Type: text/plain; charset="us-ascii" Dear Histonetters I got this request which I presume was intended for the Histonet list. Does anyone have information on this gentleman I can pass along? Dear Linda, I would appreciate it you could seek or make an inquiry for me. I am looking for an obituary and other biographical information about the histotechnologist Arthur Bolles Lee (1849 - 1927). Sincerely, Frederick H. Kasten Johnson City, TN Thanks... Linda M Histonet administrator Please consider the environment before printing this e-mail

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------------------------------ Message: 11 Date: Mon, 1 Mar 2010 17:15:23 -0800 From: Green JumpyOne Subject: [Histonet] Microwave processing and IHCs To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/ ------------------------------ Message: 12 Date: Mon, 1 Mar 2010 19:23:00 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] Microwave processing and IHCs To: , histonet Message-ID: Content-Type: text/plain; charset="Windows-1252" I have used the Sakura Xpress for 5+ years and have had great success w/ all staining, w/ little to no changes. The Xpress does not use Xylene and that was a change from our conventional processing and reagents, so we did have to collect new control tissues. I say that anytime you change the process, you MUST collect new control tissue and you will ned to document. When CAP comes to visit and they see a MW processor, you will need to show them your validation and control tissue documentation. If you have the possibility of using both conventional or Microwave processd tissue, then make a modified sausage block to include both types of processing protocols, so that work can flow through the lab smoothly. William DeSalvo, B.S., HTL(ASCP) > From: greenjumpyone@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 1 Mar 2010 17:15:23 -0800 > Subject: [Histonet] Microwave processing and IHCs > > > Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? > > We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. > > thanks for any help! > > Michelle > > _________________________________________________________________ > Hotmail: Free, trusted and rich email service. > http://clk.atdmt.com/GBL/go/201469228/direct/01/_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft?s powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/ ------------------------------ Message: 13 Date: Tue, 2 Mar 2010 11:32:29 +0800 From: "TF" Subject: Re: RE: [Histonet] CD31 in rats To: "Hernandez, Anna" , "Annette Featherstone" , "histonet@lists.utsouthwestern.edu" Message-ID: <201003021132284371600@foxmail.com> Content-Type: text/plain; charset="gb2312" Hi, we use Abcam mouse-anti CD31 also acetone fixed frozen sections. pretty well. 2010-03-02 TF ???????? Hernandez, Anna ?????????? 2010-03-02 09:50:38 ???????? Annette Featherstone; histonet@lists.utsouthwestern.edu ?????? ?????? RE: [Histonet] CD31 in rats Hello Annette, We use Purified Mouse anti-Rat CD31 from BD Biosciences (Catalog# 555025). We use on frozen sections fixed with acetone for 10 minutes with the following steps: 1X Morphosave (Ventana) (15 min) 0.3% H202 quench(Sigma) (20 min) Avidin and Biotin block (Vector) (15 min each) Covance blocking reagent (25 min) Mouse anti-Rat CD31 (25 min) at 2.0 ug/mL Biotinylated Horse anti-Mouse IgG (Vector-BA-2001) (25 minutes) at 5.0 ug/mL Covance Labeling Reagent (25 min) Covance Dab (2 x 5 min) (Each step has PBS washes in between and is performed at RT) Thanks, Anna -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone Sent: Monday, March 01, 2010 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31 in rats Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 2 Mar 2010 10:37:02 +0100 From: "Hoekert, W.E.J." Subject: RE: [Histonet] HSV I & II To: "Sebree Linda A" , "Histonet" Message-ID: <1190CB05C44B13409483514729C2FC360C0A9E@PAIT42.olvg.nl> Content-Type: text/plain; charset="iso-8859-1" Too bad that all these people did not respond to the entire group. We are using the one from Immunologic (polyclonal, ILP4114-C1). Is this one also not available anymore? Willem Hoekert OLVG, Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Sebree Linda A Verzonden: ma 1-3-2010 21:24 Aan: Histonet Onderwerp: [Histonet] HSV I & II Thanks to all who responded to my inquiry about HSV I&II however I'd like to find it in a cocktail. I have since learned that manufacturers can no longer premix ASR antibodies to sell . I guess I'll have to find out if are docs need both or can make do with one. Thanks again, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ------------------------------ Message: 15 Date: Tue, 2 Mar 2010 08:03:59 -0600 From: "Mike Pence" Subject: RE: [Histonet] Microwave processing and IHCs To: "Green JumpyOne" , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D79@is-e2k3.grhs.net> Content-Type: text/plain; charset="us-ascii" Your control tissue needs to be processed the same as your patient samples. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Monday, March 01, 2010 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing and IHCs Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 2 Mar 2010 06:03:58 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Microwave processing and IHCs To: histonet@lists.utsouthwestern.edu, Green JumpyOne Message-ID: <458340.86147.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Michelle: The key in MW tissue processing is to accelerate the process and it is very likely that the quality of the tissues will be the same as if processed in a more "traditional" way, at least that is the claim and the objective. Having said that this does not mean that you don't have to demonstrate it with a validation process. You should always have controls processed in the very same way as the patients' tissues so in theory you have to obtain a new set of controls processed with the your MW tissue processor. The complication develops if you have also a "conventional" tissue processor and then you will need two sets of controls. Ideally all your cases should be processed with a single method. Ren? J. --- On Mon, 3/1/10, Green JumpyOne wrote: From: Green JumpyOne Subject: [Histonet] Microwave processing and IHCs To: histonet@lists.utsouthwestern.edu Date: Monday, March 1, 2010, 8:15 PM Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed.? If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle ??? ???????? ?????? ??? ? _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Tue, 2 Mar 2010 09:15:11 -0500 From: "McMahon, Loralee A" Subject: RE: [Histonet] Microwave processing and IHCs To: Mike Pence , Green JumpyOne , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" In my experience I have not seen any change in the MW tissue versus the routine processing. What we have tried to do since we have so many different antibodies and control tissues is create mini sausage blocks with one piece of MW tissue and one piece of non MW tissue. Because we were not always sure what tissue were microwaved and what tissues were not. For example one small piece of tonsil microwaved and one non microwaved embedded in the same block. That way no matter what your patient tissue sample has gone through you have all the bases covered. It takes a long time to set up but will save you time in the end. If you run your tissues when you validate the microwave you'll have half of the process finished. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpence@grhs.net] Sent: Tuesday, March 02, 2010 9:03 AM To: Green JumpyOne; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing and IHCs Your control tissue needs to be processed the same as your patient samples. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Monday, March 01, 2010 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing and IHCs Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Tue, 2 Mar 2010 08:37:49 -0600 From: "Vickroy, Jim" Subject: FW: [Histonet] Microwave processing and IHCs To: "Histonet@lists.utsouthwestern.edu" Message-ID: <24A4826E8EF0964D86BC5317306F58A54257908A0A@mmc-mail.ad.mhsil.com> Content-Type: text/plain; charset="us-ascii" See below James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: Vickroy, Jim Sent: Tuesday, March 02, 2010 8:16 AM To: 'Mike Pence' Subject: RE: [Histonet] Microwave processing and IHCs I understand and agree in principle. However let me ask this: If a validation study shows that there is no difference between conventional tissue processing and microwave tissue processing in your IHC protocols then why can't you then use controls that are processed either by conventional or microwave. If we can't then are we using controls that have been decaled for tissues that have to be decaled and so forth. I have always believed if you can validate there is no appreciable difference in methods than we can use the same controls. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, March 02, 2010 8:04 AM To: Green JumpyOne; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing and IHCs Your control tissue needs to be processed the same as your patient samples. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Monday, March 01, 2010 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing and IHCs Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 19 Date: Tue, 2 Mar 2010 08:49:50 -0600 From: "Horn, Hazel V" Subject: RE: [Histonet] inquiry about Arthur Bolles Lee To: "LINDA MARGRAF" , Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83738@EMAIL.archildrens.org> Content-Type: text/plain; charset="us-ascii" If you plug his name into google several links appear.. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Monday, March 01, 2010 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inquiry about Arthur Bolles Lee Dear Histonetters I got this request which I presume was intended for the Histonet list. Does anyone have information on this gentleman I can pass along? Dear Linda, I would appreciate it you could seek or make an inquiry for me. I am looking for an obituary and other biographical information about the histotechnologist Arthur Bolles Lee (1849 - 1927). Sincerely, Frederick H. Kasten Johnson City, TN Thanks... Linda M Histonet administrator Please consider the environment before printing this e-mail

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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 2 *************************************** From flnails <@t> texaschildrens.org Tue Mar 2 11:43:42 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Mar 2 11:46:21 2010 Subject: [Histonet] IHC In-Reply-To: References: Message-ID: Joe, I wanted to ask you a question about which type of IHC stainer you are using and which stainer do you think is the user friendly and efficient. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, February 26, 2010 4:51 PM To: CHRISTIE GOWAN; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fire in the lab Once upon a time in a far away land, we used to boil our embedding molds in boiling soapy water, over an open Bunsen burner, followed by an alcohol rinse then air dry. One time the fire alarm was activated and we had to evacuate the hospital. We were out there quit awhile. When we received the all clear to go back into the hospital, I was the first one back in the lab and the fire department was there, looking into our pot that had boiled out and was smoking up the lab. This wasn't the cause of the first alarm, but it did set off the second. Joe ----- Original Message ----- From: "CHRISTIE GOWAN" To: Sent: Friday, February 26, 2010 8:20 AM Subject: [Histonet] Fire in the lab Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if this type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From wlecorch <@t> rwjuhh.edu Tue Mar 2 12:47:48 2010 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Tue Mar 2 12:47:59 2010 Subject: [Histonet] Re Billing for H&E stains Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71A4AB78B67@HAMEXMBA.rwjham.local> It is my understanding that CPT 88173 for Dx of FNA covers your stains Diff Quick, H&E, PAP you can add 88305 for a cell block but not charge for each stain. From Mark.Elliott <@t> hli.ubc.ca Tue Mar 2 12:51:41 2010 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Tue Mar 2 12:54:16 2010 Subject: [Histonet] Deleting extraneous parts of posts when replying In-Reply-To: <0DFDC8B4.719@mail.mrl.ubc.ca> References: <0DFDC8B4.719@mail.mrl.ubc.ca> Message-ID: <4B8CEDBD020000D60003A3D9@mail.mrl.ubc.ca> Just a friendly reminder that if you are responding to a question to please delete out everything but the pertinent information, especially if you are receiving/responding in Digest mode. If you don't cut out the irrelevant posts it makes it extremely hard to find the new stuff. Some of the Digest versions that have come out in the last few days have Digests within Digests within Digests so it makes the email really long and you can't find the new stuff in amongst the old. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From Farnana <@t> nehealth.com Tue Mar 2 13:12:04 2010 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Tue Mar 2 13:12:16 2010 Subject: [Histonet] bone marrow fixative Message-ID: <4B8D1CB4.26ED.00D9.1@nehealth.com> Good afternoon everyone, In past years our lab used B-5 fixative on bone marrow biopsies for better immunostaining. With the chemical hazards of B-5 we switched to AZF fixative and have been using this for the past few years. I am just curious what everyone else is using for fixative on their bone marrows and how is your immunoreactivity? Maybe a better question is what is the preferred fixative for bone marrows? Have a nice day, Amy Farnan Histology Supervisor Northeast Health Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From GauchV <@t> mail.amc.edu Tue Mar 2 13:20:24 2010 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Tue Mar 2 13:20:34 2010 Subject: [Histonet] H&E stainer/coverslippers Message-ID: Hi, I was wondering what type of H&E stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our "old faithful" GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From carrolpb <@t> umdnj.edu Tue Mar 2 13:38:31 2010 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Tue Mar 2 13:38:42 2010 Subject: [Histonet] Deleting extraneous parts of posts when replying In-Reply-To: <4B8CEDBD020000D60003A3D9@mail.mrl.ubc.ca> References: <0DFDC8B4.719@mail.mrl.ubc.ca> <4B8CEDBD020000D60003A3D9@mail.mrl.ubc.ca> Message-ID: <4B8D6937.9010006@umdnj.edu> given how many people cant even figure out how to properly unsubscribe from this list, i fear your request might be in vain ;-) Mark Elliott wrote: > Just a friendly reminder that if you are responding to a question to please delete out everything but the pertinent information, especially if you are receiving/responding in Digest mode. If you don't cut out the irrelevant posts it makes it extremely hard to find the new stuff. Some of the Digest versions that have come out in the last few days have Digests within Digests within Digests so it makes the email really long and you can't find the new stuff in amongst the old. > > Thanks > > Mark > > ***CONFIDENTIALITY NOTICE*** > This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From dchihc <@t> yahoo.com Tue Mar 2 13:43:40 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Tue Mar 2 13:43:44 2010 Subject: [Histonet] HistoTech Position at DCH Regional in Tuscaloosa, AL Message-ID: <474963.63078.qm@web43505.mail.sp1.yahoo.com> At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday.?Must be ASCP registered HT or HTL. Lab Automation in the lab includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura?XPress 50 rapid tissue processor, Leica cryostats. DCH is a 500 plus bed regional medical center. Interested candidates please contact Sherrie Faulkner?(recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From laurie.colbert <@t> huntingtonhospital.com Tue Mar 2 14:10:40 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Mar 2 14:10:44 2010 Subject: [Histonet] H&E stainer/coverslippers Message-ID: <57BE698966D5C54EAE8612E8941D7683080981B1@EXCHANGE3.huntingtonhospital.com> We have the Sakura Prisma and attached film coverslipper. We love both!! Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Tuesday, March 02, 2010 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E stainer/coverslippers Hi, I was wondering what type of H&E stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our "old faithful" GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue Mar 2 14:34:56 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Mar 2 14:35:21 2010 Subject: [Histonet] H&E stainer/coverslippers In-Reply-To: Message-ID: <95A4872A68BC4D74BC0C5545EEDED85E@lurie.northwestern.edu> Vicki, We just ordered the Leica combo.We have the separate units of the Autostainer XL and CV5030. They now sell as a combo (a transfer station moves the racks) As we've relied on Leica for years, I expect we will be happy with the new unit. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Tuesday, March 02, 2010 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E stainer/coverslippers Hi, I was wondering what type of H&E stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our "old faithful" GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 2 14:38:23 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 2 14:38:26 2010 Subject: [Histonet] bone marrow fixative In-Reply-To: <4B8D1CB4.26ED.00D9.1@nehealth.com> Message-ID: <709753.38567.qm@web65704.mail.ac4.yahoo.com> Buffered 10% NBF, but with the pH exactly at 7.0, checked for each case. IHC is perfect. Ren? J. --- On Tue, 3/2/10, Amy Farnan wrote: From: Amy Farnan Subject: [Histonet] bone marrow fixative To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 2, 2010, 2:12 PM Good afternoon everyone, In past years our lab used B-5 fixative on bone marrow biopsies for better immunostaining. With the chemical hazards of B-5 we switched to AZF fixative and have been using this for the past few years. I am just curious what everyone else is using for fixative on their bone marrows and how is your immunoreactivity?? Maybe a better question is what is the preferred fixative for bone marrows? Have a nice day, Amy Farnan Histology Supervisor Northeast Health Disclaimer: The information in this message is confidential.? If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 2 14:39:04 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 2 14:39:08 2010 Subject: [Histonet] H&E stainer/coverslippers In-Reply-To: Message-ID: <609190.61592.qm@web65714.mail.ac4.yahoo.com> Sakura Ren? J. --- On Tue, 3/2/10, Gauch, Vicki wrote: From: Gauch, Vicki Subject: [Histonet] H&E stainer/coverslippers To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 2, 2010, 2:20 PM Hi, I was wondering what type of H&E stainer/coverslipper units labs are using? and the pros and cons of each.? We found out we have funding to replace our "old faithful" GLX stainer(though it will break my heart to do so)? and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper.? Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Mar 2 14:50:12 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Mar 2 14:50:17 2010 Subject: [Histonet] H&E stainer/coverslippers In-Reply-To: <609190.61592.qm@web65714.mail.ac4.yahoo.com> Message-ID: We just purchased the sakura prisma and attached glass coverslipper. We just love them. We tried a lot of glass coverslippers and this by far is the best one that we have tried. We run both H&E and specials on the prisma and have not had a problem with the glass coverslipper. We have been using them for about a little over 2 months now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 02, 2010 1:39 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: Re: [Histonet] H&E stainer/coverslippers Sakura Ren? J. --- On Tue, 3/2/10, Gauch, Vicki wrote: From: Gauch, Vicki Subject: [Histonet] H&E stainer/coverslippers To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 2, 2010, 2:20 PM Hi, I was wondering what type of H&E stainer/coverslipper units labs are using? and the pros and cons of each.? We found out we have funding to replace our "old faithful" GLX stainer(though it will break my heart to do so)? and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper.? Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Tue Mar 2 14:50:25 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Tue Mar 2 14:50:29 2010 Subject: [Histonet] HistoTech Position At DCH In Tuscaloosa, Alabama (Phone contact correction) Message-ID: <289719.13101.qm@web43515.mail.sp1.yahoo.com> At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday.?Must be ASCP registered HT or HTL. Lab Automation in the lab includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura?XPress 50 rapid tissue processor, Leica cryostats. DCH is a 500 plus bed regional medical center. Interested candidates please contact Sherrie Faulkner?(recruiter) at 205-750-5736 or fax resume to Michelle Fagin at 205-750-5224. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL? ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From HornHV <@t> archildrens.org Tue Mar 2 15:14:50 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Mar 2 15:14:54 2010 Subject: [Histonet] H&E stainer/coverslippers In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8373F@EMAIL.archildrens.org> We have Leica for both and love them.. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Tuesday, March 02, 2010 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E stainer/coverslippers Hi, I was wondering what type of H&E stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our "old faithful" GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From gankam <@t> googlemail.com Tue Mar 2 15:25:33 2010 From: gankam <@t> googlemail.com (Fabrice gankam) Date: Tue Mar 2 15:25:40 2010 Subject: [Histonet] Deleting extraneous parts of posts when replying In-Reply-To: <4B8CEDBD020000D60003A3D9@mail.mrl.ubc.ca> References: <0DFDC8B4.719@mail.mrl.ubc.ca> <4B8CEDBD020000D60003A3D9@mail.mrl.ubc.ca> Message-ID: <86c37d601003021325x5aff7c06s38682936b8edd792@mail.gmail.com> Hi, Just wondering if anyone has use hydroethinide to detect the free radicals (ROS) in the CNS or anyother tissue of rats after 4% formallin fixation and parrafin embedding. I wanted to used the hydroethidine on paraffin sections. I wonder if the fluorescence is lost by fixation (12hrs in 4% PAF) and paraffin embedding plus deparaffination. All the papers I reviewed used hydroethidine on frozen section but our facility does not have vibratome or cryostat. Please help Please Help. Fabrice From trathborne <@t> somerset-healthcare.com Tue Mar 2 15:32:46 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Mar 2 15:32:55 2010 Subject: [Histonet] bone marrow fixative In-Reply-To: <4B8D1CB4.26ED.00D9.1@nehealth.com> Message-ID: We use zinc formalin for the bone marrow specimens, and the pathologists are satisfied with the results. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Amy Farnan Sent: Tuesday, March 02, 2010 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow fixative Good afternoon everyone, In past years our lab used B-5 fixative on bone marrow biopsies for better immunostaining. With the chemical hazards of B-5 we switched to AZF fixative and have been using this for the past few years. I am just curious what everyone else is using for fixative on their bone marrows and how is your immunoreactivity? Maybe a better question is what is the preferred fixative for bone marrows? Have a nice day, Amy Farnan Histology Supervisor Northeast Health Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From trathborne <@t> somerset-healthcare.com Tue Mar 2 15:43:09 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Mar 2 15:43:16 2010 Subject: [Histonet] H&E stainer/coverslippers In-Reply-To: <95A4872A68BC4D74BC0C5545EEDED85E@lurie.northwestern.edu> Message-ID: We have the Leica CV5030 and ST5020 with the transfer station and are very pleased with them. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bernice Frederick Sent: Tuesday, March 02, 2010 3:35 PM To: 'Gauch, Vicki'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E stainer/coverslippers Vicki, We just ordered the Leica combo.We have the separate units of the Autostainer XL and CV5030. They now sell as a combo (a transfer station moves the racks) As we've relied on Leica for years, I expect we will be happy with the new unit. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Tuesday, March 02, 2010 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E stainer/coverslippers Hi, I was wondering what type of H&E stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our "old faithful" GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From POWELL_SA <@t> mercer.edu Tue Mar 2 16:00:22 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Mar 2 16:00:29 2010 Subject: [Histonet] National Histotechnology Professionals Day Message-ID: <9BF995BC0E47744E9673A41486E24EE2242A453A29@MERCERMAIL.MercerU.local> Yesterday Georgia Governor Sonny Perdue signed a proclamation declaring March 10, 2010 the first National Histotechnology Professionals Day in Georgia. Go to www.histosearch.com/gsh to see a great photo of this memorable occasion for the histology professionals of Georgia on our home page. I hope everyone will seize the opportunity to inform our communities of our contribution to their tissue diagnoses and treatment. While you at the website go to the symposium page and register to attend our meeting March 26-27 to be held at the Evergreen Marriott Convention Resort, Stone Mountain, GA. The program, registration form, and a direct link to the hotel with meeting code already entered for your convenience. Just finish the reservation information. Our speakers include James Burchette, Joe Myers, Damien Laudier, Lualhati Harkins, Marvin Hanna, Gina Rodriguez, Kris Fidler, and Jack Ratliff. Make your plans now to attend. Deadline for registration without a late fee is March 15th. Shirley A. Powell, HT(ASCP)HTL, QIHC GSH Secretary Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From bitesizellama <@t> gmail.com Tue Mar 2 18:32:53 2010 From: bitesizellama <@t> gmail.com (Mauricio Avigdor) Date: Tue Mar 2 18:32:57 2010 Subject: [Histonet] IF staining on peritoneal macrophages Message-ID: <1b2831cd1003021632g6dd48430n169c339a2316ac5d@mail.gmail.com> Hello Histoneters, I am very happy to say that my cytospins are finally working and that cells are staying on the slides throughout the procedure. Thank you very much to all who chimed in or helped with trial samples. I was almost ready to declare victory when I noticed one of my negative control slides looked a bit much like it has specific staining. The antibodies I have been using have not been giving the most even results. Does anyone have a recommendation for an anti-mouse F4/80 antibody? And a matching FITC-conjugated secondary antibody? Is anyone here doing IF staining of mouse peritoneal macrophages? From jnocito <@t> satx.rr.com Tue Mar 2 18:52:07 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Mar 2 18:52:36 2010 Subject: [Histonet] Alcohol Source? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46EE8@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46EE8@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <8F48E360671943E38662E52001109611@JoePC> depends. A fine Scotch whiskey may be aged in old oak casks, while a delicate Tequila may not be ----- Original Message ----- From: "Breeden, Sara" To: "histonet" Sent: Tuesday, March 02, 2010 10:44 AM Subject: [Histonet] Alcohol Source? I hate to even ADMIT this and I ought to use a disguise when asking - but is alcohol a wood-based solution? I should know this - I know... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Tue Mar 2 19:08:23 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Mar 2 19:08:28 2010 Subject: [Histonet] Re: Ultram fixative Message-ID: Richard Cartun asks >>We have a clinic that is fixing tissue in "Ultram". I have never heard of it. Can someone educate me about "Ultram"?<< I can't Google any evidence for an Ultram fixative. Ultram is a trade name for tramadol, an opioid analgesic. Either somebody is confused, or you've got somebody involved in dubious drug transactions. Can anybody in the clinic shed any light on the matter? Bob Richmond Samurai Pathologist Knoxville TN From andrespepo82 <@t> yahoo.com Tue Mar 2 19:32:03 2010 From: andrespepo82 <@t> yahoo.com (andres garcia) Date: Tue Mar 2 19:32:06 2010 Subject: [Histonet] Looking for Lab Aid position Message-ID: <547565.11160.qm@web38307.mail.mud.yahoo.com> Hi everybody; I'm an experienced Lab Aid looking for a job in the Central Florida area (Orlando). I have worked in Histology for over 3 years, and I'm very familiar with all the equipment and the everyday routine of the Lab. I can furnish a full resume on request. I'd appreciate any pointers anyone could offer. Andres Garcia andrespepo82 at yahoo dot com From jkiernan <@t> uwo.ca Wed Mar 3 00:43:35 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Mar 3 00:43:41 2010 Subject: [Histonet] bone marrow fixative In-Reply-To: <4B8D1CB4.26ED.00D9.1@nehealth.com> References: <4B8D1CB4.26ED.00D9.1@nehealth.com> Message-ID: What is AZF? ----- Original Message ----- From: Amy Farnan Date: Tuesday, March 2, 2010 14:29 Subject: [Histonet] bone marrow fixative To: histonet@lists.utsouthwestern.edu > Good afternoon everyone, > > In past years our lab used B-5 fixative on bone marrow biopsies > for better immunostaining. > With the chemical hazards of B-5 we switched to AZF fixative and > have been using this for the past few years. I am just curious > what everyone else is using for fixative on their bone marrows > and how is your immunoreactivity? Maybe a better question > is what is the preferred fixative for bone marrows? > > Have a nice day, > Amy Farnan > Histology Supervisor > Northeast Health > > Disclaimer: The information in this message is > confidential. If you are not the intended recipient, do > not disclose, copy, or distribute this message, and please > immediately contact the sender. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Mar 3 00:48:12 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Mar 3 00:48:16 2010 Subject: [Histonet] ROS Message-ID: Reactive oxygen species (ROS) are unstable ions or free radicals including superoxide, peroxynitrite and nitric oxide. Most exist for milliseconds before combining with other substances. Nitric oxide is exceptional because it can move about through cytoplasm, cell membranes and extracellular space for several tenths of a second before being changed into much more short-lived ROS. The fluorescent methods for ROS are for instant photography of cell cultures. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Fabrice gankam Date: Tuesday, March 2, 2010 16:26 Subject: Re: [Histonet] Deleting extraneous parts of posts when replying To: histonet@lists.utsouthwestern.edu > Hi, > Just wondering if anyone has use hydroethinide to detect > the free radicals > (ROS) in the CNS or anyother tissue of rats after 4% formallin > fixation and > parrafin embedding. > I wanted to used the hydroethidine on paraffin sections. > I wonder if the fluorescence is lost by fixation (12hrs in 4% > PAF) and > paraffin embedding plus deparaffination. > All the papers I reviewed used hydroethidine on frozen section > but our > facility does not have vibratome or cryostat. > Please help > Please Help. > > Fabrice > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Wed Mar 3 07:57:22 2010 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Mar 3 07:57:45 2010 Subject: [Histonet] AZF Message-ID: <8CC88F095232A89-339C-C4EA@webmail-m047.sysops.aol.com> Dr Kiernan asks about AZF When the safety people in our medical center finally pried the last of the B-5 from my cold, dead, hand, we replaced it with AZF which contains formaldehyde, zinc chloride, methanol and acetic acid. Our hematopathologist claims it is better than straight zinc formalin, and, until recently, it was available in prefilled containers making it perfect for dishing out to clinics and floors etc. It works almost as well as B-5. It is sold in the USA by Newcomer supply. You still cannot pour it down the sink after use, but is not so dangerous as mercury containing B-5. Michael Titford Pathology USA Mobile AL USA From rsrichmond <@t> gmail.com Wed Mar 3 08:05:04 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Mar 3 08:05:10 2010 Subject: [Histonet] Re: bone marrow fixative Message-ID: Now that we can no longer use mercury-containing fixatives, I don't think that the various proprietary fixatives advocated for bone marrow add anything to the morphology, and some of them can gum up processors or interfere with immunohistochemistry. Neutral buffered formalin requires time for fixation - clots need to be cut up as soon as possible after they're received, and biopsy specimens really ought to fix overnight before decalcification and processing. Communication with oncologists is essential (and rarely achievable). Bob Richmond Samurai Pathologist Knoxville TN From leiker <@t> buffalo.edu Wed Mar 3 08:25:40 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Mar 3 08:25:46 2010 Subject: [Histonet] Deleting extraneous parts of posts when replying In-Reply-To: <86c37d601003021325x5aff7c06s38682936b8edd792@mail.gmail.com> References: <0DFDC8B4.719@mail.mrl.ubc.ca> <4B8CEDBD020000D60003A3D9@mail.mrl.ubc.ca> <86c37d601003021325x5aff7c06s38682936b8edd792@mail.gmail.com> Message-ID: <973F09E0B57A0A9506674396@CDYwxp1931.ad.med.buffalo.edu> My brief experience with paraffin and fluorescence (DAPI and EGFP) is that the paraffin embedding process destroys it. Regards, Merced --On Tuesday, March 02, 2010 3:25 PM -0600 Fabrice gankam wrote: > Hi, > Just wondering if anyone has use hydroethinide to detect the free > radicals (ROS) in the CNS or anyother tissue of rats after 4% formallin > fixation and parrafin embedding. > I wanted to used the hydroethidine on paraffin sections. > I wonder if the fluorescence is lost by fixation (12hrs in 4% PAF) and > paraffin embedding plus deparaffination. > All the papers I reviewed used hydroethidine on frozen section but our > facility does not have vibratome or cryostat. > Please help > Please Help. > > Fabrice > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From FARNANA <@t> nehealth.com Wed Mar 3 08:27:52 2010 From: FARNANA <@t> nehealth.com (Amy Farnan) Date: Wed Mar 3 08:28:01 2010 Subject: [Histonet] bone marrow fixative In-Reply-To: References: <4B8D1CB4.26ED.00D9.1@nehealth.com> Message-ID: <4B8E2B98.73AC.00D9.1@nehealth.com> Acetic Zinc Formalin Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From greenjumpyone <@t> hotmail.com Wed Mar 3 09:46:52 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Wed Mar 3 09:46:56 2010 Subject: [Histonet] RE: B5 fixative In-Reply-To: References: Message-ID: Amy, We use the B+ fixative available from BBC Biochemical ( http://www.bbcus.com/products.html?pc=19&pid=57 ) My pathologists love it! Michelle > Message: 3 > Date: Tue, 02 Mar 2010 14:12:04 -0500 > From: "Amy Farnan" > Subject: [Histonet] bone marrow fixative > To: > Message-ID: <4B8D1CB4.26ED.00D9.1@nehealth.com> > Content-Type: text/plain; charset=US-ASCII > > Good afternoon everyone, > > In past years our lab used B-5 fixative on bone marrow biopsies for better immunostaining. > With the chemical hazards of B-5 we switched to AZF fixative and have been using this for the past few years. I am just curious what everyone else is using for fixative on their bone marrows and how is your immunoreactivity? Maybe a better question is what is the preferred fixative for bone marrows? > > Have a nice day, > Amy Farnan > Histology Supervisor > Northeast Health > *************************************** _________________________________________________________________ Hotmail: Trusted email with Microsoft?s powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/ From greenjumpyone <@t> hotmail.com Wed Mar 3 09:52:23 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Wed Mar 3 09:53:59 2010 Subject: [Histonet] H&E stainer/coverslippers In-Reply-To: References: Message-ID: We are using the Leica Autostainer XL, bridge and coverslipper (CV5030). I think they're great! Michelle _________________________________________________________________ Hotmail: Trusted email with Microsoft?s powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/ From andreahooper <@t> rocketmail.com Wed Mar 3 10:09:07 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Wed Mar 3 10:09:11 2010 Subject: [Histonet] IF staining on peritoneal macrophages Message-ID: <494570.77414.qm@web113105.mail.gq1.yahoo.com> Rat anti-mouse F480 from serotec works hwell. It is crucial that you use a secondary against rat IgG that is highly cross adsorbed to many species, including mouse. I suggest whole IgG of donkey anti-rat IgG from Jackson Immunoresearch. Just make sure to pick the version adsorbed against mouse. I would give you the catalog number except I am not at my computer. Andrea T. Hooper From andreahooper <@t> rocketmail.com Wed Mar 3 10:09:06 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Wed Mar 3 10:11:04 2010 Subject: [Histonet] IF staining on peritoneal macrophages Message-ID: <33669.27581.qm@web113103.mail.gq1.yahoo.com> Rat anti-mouse F480 from serotec works hwell. It is crucial that you use a secondary against rat IgG that is highly cross adsorbed to many species, including mouse. I suggest whole IgG of donkey anti-rat IgG from Jackson Immunoresearch. Just make sure to pick the version adsorbed against mouse. I would give you the catalog number except I am not at my computer. Andrea T. Hooper From sariyad <@t> hotmail.com Wed Mar 3 09:41:03 2010 From: sariyad <@t> hotmail.com (Dinesh Sariya) Date: Wed Mar 3 10:11:08 2010 Subject: [Histonet] Processing of fatty breast tissue Message-ID: Following adequate fixation of predominantly fatty breast tissue, what factors could lead to significant shrinkage of adipose tissue around a fibrous lesion (by ~ 50-80%) ? _________________________________________________________________ IM on the go with Messenger on your phone http://go.microsoft.com/?linkid=9712960 From rjbuesa <@t> yahoo.com Wed Mar 3 10:20:59 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 3 10:21:03 2010 Subject: [Histonet] Processing of fatty breast tissue In-Reply-To: Message-ID: <954014.91205.qm@web65706.mail.ac4.yahoo.com> You have assumed that processing is correct, but shrinkage is always caused by too fast dehydration, therefore the processing is not adequate. Ren? J. --- On Wed, 3/3/10, Dinesh Sariya wrote: From: Dinesh Sariya Subject: [Histonet] Processing of fatty breast tissue To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 3, 2010, 10:41 AM Following adequate fixation of predominantly fatty breast tissue, what factors could lead to significant shrinkage of adipose tissue around a fibrous lesion (by ~ 50-80%) ?? ??? ???????? ?????? ??? ? _________________________________________________________________ IM on the go with Messenger on your phone http://go.microsoft.com/?linkid=9712960_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gankam <@t> googlemail.com Wed Mar 3 10:43:35 2010 From: gankam <@t> googlemail.com (Fabrice GANKAM) Date: Wed Mar 3 10:43:54 2010 Subject: [Histonet] Free radical detection and cell death IHC In-Reply-To: <973F09E0B57A0A9506674396@CDYwxp1931.ad.med.buffalo.edu> References: <0DFDC8B4.719@mail.mrl.ubc.ca> <4B8CEDBD020000D60003A3D9@mail.mrl.ubc.ca> <86c37d601003021325x5aff7c06s38682936b8edd792@mail.gmail.com> <973F09E0B57A0A9506674396@CDYwxp1931.ad.med.buffalo.edu> Message-ID: Sorry to post this again but had problems posting messages lately. Here is my problem, We do not have cryostat in our facility and I will like to test free radical production in situ in rat brain tissue Some of the methods involves homogenate of whole part of brain and assay with component reacting with free radical that does not give you the geographical distribution of free radical. I'm therefore looking for a methods involving IHC or IF in paraffin section. I checked for hydroethidine but it seems like the fluorescence fades with paraffin processing. Has any one used hydroethidine with paraffin processing ? Does anyone know another method of free radical or oxidative stress detection on paraffin section ? We also wanted to asses cell death by some marker with the same tissue (paraffin processed) the caspase stain is faint and we will like to find a selective marker of cellular death or irreversible damage (apoptotic or non apoptotic) that could be used in IHC and IF. Has anyone ever used PI on rat tissue after paraffin processing ? Any other marker ? I heard about the hydroxyprobe but does it work on paraffin embedded tissue ? Thanks for your help guys Fabrice From plucas <@t> biopath.org Wed Mar 3 10:50:06 2010 From: plucas <@t> biopath.org (Paula Lucas) Date: Wed Mar 3 10:47:46 2010 Subject: [Histonet] H&E stainer/coverslippers In-Reply-To: References: <609190.61592.qm@web65714.mail.ac4.yahoo.com> Message-ID: <009101cabaf1$94230c50$0f01a8c0@biopath.local> I second the Prisma with attached coverslipper. We have been using it now for the past 2 months or so. It's really a non-hassle, state of the art piece of equipment. Paula -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, March 02, 2010 12:50 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: RE: [Histonet] H&E stainer/coverslippers We just purchased the sakura prisma and attached glass coverslipper. We just love them. We tried a lot of glass coverslippers and this by far is the best one that we have tried. We run both H&E and specials on the prisma and have not had a problem with the glass coverslipper. We have been using them for about a little over 2 months now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 02, 2010 1:39 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: Re: [Histonet] H&E stainer/coverslippers Sakura Ren? J. --- On Tue, 3/2/10, Gauch, Vicki wrote: From: Gauch, Vicki Subject: [Histonet] H&E stainer/coverslippers To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 2, 2010, 2:20 PM Hi, I was wondering what type of H&E stainer/coverslipper units labs are using? and the pros and cons of each.? We found out we have funding to replace our "old faithful" GLX stainer(though it will break my heart to do so)? and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper.? Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From integrated.histo <@t> gmail.com Wed Mar 3 11:15:16 2010 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Wed Mar 3 11:15:23 2010 Subject: [Histonet] Ultram Fixative Message-ID: <5d9104a31003030915g6d953003h1e36f0918246da77@mail.gmail.com> "Ultram" Fixative is actually Ultrum Fixative and is available thru American Mastertech: http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=FXULTGAL Cindy Dubois Message: 20 Date: Tue, 2 Mar 2010 20:08:23 -0500 From: Robert Richmond Subject: [Histonet] Re: Ultram fixative To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Richard Cartun asks >>We have a clinic that is fixing tissue in "Ultram". I have never heard of it. Can someone educate me about "Ultram"?<< I can't Google any evidence for an Ultram fixative. Ultram is a trade name for tramadol, an opioid analgesic. Either somebody is confused, or you've got somebody involved in dubious drug transactions. Can anybody in the clinic shed any light on the matter? Bob Richmond Samurai Pathologist Knoxville TN From azdudley <@t> hotmail.com Wed Mar 3 11:44:20 2010 From: azdudley <@t> hotmail.com (anita dudley) Date: Wed Mar 3 11:44:27 2010 Subject: [Histonet] negative controls Message-ID: we have a ventana benchmark and use rabbit and mouse negative controls, is there anyone out there that uses a universal control and is that ok with your cap inspection? thanks so much antia dudley providence hosp mobile alabama _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/ From LSebree <@t> uwhealth.org Wed Mar 3 12:07:46 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Mar 3 12:08:47 2010 Subject: [Histonet] negative controls In-Reply-To: Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF7CC@UWHC-MAIL01.uwhis.hosp.wisc.edu> Anita, If you're referring to the reagents you use, we use a Universal Negative Control Serum (Biocare) on our negative control slides. We have 3 Ventana instruments and have never been cited. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, March 03, 2010 11:44 AM To: histonet@pathology.swmed.edu Subject: [Histonet] negative controls we have a ventana benchmark and use rabbit and mouse negative controls, is there anyone out there that uses a universal control and is that ok with your cap inspection? thanks so much antia dudley providence hosp mobile alabama _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Wed Mar 3 12:58:42 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Wed Mar 3 12:58:47 2010 Subject: [Histonet] H&E stainer/coverslippers In-Reply-To: <009101cabaf1$94230c50$0f01a8c0@biopath.local> References: <609190.61592.qm@web65714.mail.ac4.yahoo.com> <009101cabaf1$94230c50$0f01a8c0@biopath.local> Message-ID: <293340.83444.qm@web43510.mail.sp1.yahoo.com> I am glad you decided on that one Paula. It is wonderful!!!! ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: Paula Lucas To: Liz Chlipala ; histonet@lists.utsouthwestern.edu; VickiGauch Sent: Wed, March 3, 2010 10:50:06 AM Subject: RE: [Histonet] H&E stainer/coverslippers I second the Prisma with attached coverslipper.? We have been using it now for the past 2 months or so. It's really a non-hassle, state of the art piece of equipment. Paula -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, March 02, 2010 12:50 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: RE: [Histonet] H&E stainer/coverslippers We just purchased the sakura prisma and attached glass coverslipper.? We just love them.? We tried a lot of glass coverslippers and this by far is the best one that we have tried.? We run both H&E and specials on the prisma and have not had a problem with the glass coverslipper.? We have been using them for about a little over 2 months now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 02, 2010 1:39 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: Re: [Histonet] H&E stainer/coverslippers Sakura Ren? J. --- On Tue, 3/2/10, Gauch, Vicki wrote: From: Gauch, Vicki Subject: [Histonet] H&E stainer/coverslippers To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 2, 2010, 2:20 PM Hi, I was wondering what type of H&E stainer/coverslipper units labs are using? and the pros and cons of each.? We found out we have funding to replace our "old faithful" GLX stainer(though it will break my heart to do so)? and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper.? Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LBenedetti <@t> leloir.org.ar Wed Mar 3 13:34:45 2010 From: LBenedetti <@t> leloir.org.ar (Lorena Benedetti) Date: Wed Mar 3 13:34:58 2010 Subject: [Histonet] AutoZyme Message-ID: <0D913AE8FD19114CA45F61FD97636CD8D62DA2@MUNICH> Hi, I am from Argentina and I have to perform an immunehistochemstry with an antigen recovery using AutoZyme from Biomedia Foster City, CA. I looked for in google and I found a lot papers citations but I could find the company, Biomedia, to ask for a quotation. Has anybody got a contact from this company? Or an alternative product? Thanks in advance. Lorena Lic. Lorena Bendetti Laboratori de Terapia Molecular y Celular Fundacion Instituto Leloir Buenos Aires Argentina From John.McGinley <@t> ColoState.EDU Wed Mar 3 13:56:47 2010 From: John.McGinley <@t> ColoState.EDU (McGinley,John) Date: Wed Mar 3 13:56:53 2010 Subject: [Histonet] Colorado Society of Histotechnology meeting - April 23rd & 24th in Estes Park Message-ID: Hi, The Colorado Society of Histotechnology (CSH) meeting will be held at the YMCA or Rockies in Estes Park, CO, April 23rd & 24th, 2010. If you are interested in attending the meeting and have not registered, please visit our website at http://www.coloradohisto.org/2010/meeting.htm. Online registration is available and the society is now able to take credit card payment. Thank you and I look forward to seeing you at the meeting. Regards, John McGinley CSH Secretary ----------------------------- John N. McGinley Cancer Prevention Laboratory Colorado State University 1173 Campus Delivery Fort Collins, CO 80523-1173 Ph: (970) 491-3041 Fx: (970) 491-3542 john.mcginley@colostate.edu www.cpl.colostate.edu From bitesizellama <@t> gmail.com Wed Mar 3 14:18:47 2010 From: bitesizellama <@t> gmail.com (Mauricio Avigdor) Date: Wed Mar 3 14:18:52 2010 Subject: [Histonet] IF staining on peritoneal macrophages In-Reply-To: <1b2831cd1003031027x273a5edatae9dee6a1087cc1c@mail.gmail.com> References: <33669.27581.qm@web113103.mail.gq1.yahoo.com> <1b2831cd1003031027x273a5edatae9dee6a1087cc1c@mail.gmail.com> Message-ID: <1b2831cd1003031218g4bbf2446kef5daebdfcd260d7@mail.gmail.com> Hi Andrea, Jackson lists a couple of FITC-conjugated donkey anti-rat secondaries: 712-095-150 - Whole Donkey Anti-Rat IgG (H+L). 712-096-150 - F(ab')2 fragment Donkey Anti-Rat IgG (H+L). Do you recognize which is the one you have ben using. I have been using Serotec's STAR80F antibody. They suggest using 1:10 Normal Mouse Serum in PBS to make the necessary dilutions. Is this something you do with the Jackson antibodies as well? I've never heard of this technique before. From jkiernan <@t> uwo.ca Wed Mar 3 14:36:48 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Mar 3 14:36:57 2010 Subject: [Histonet] Ultram Fixative Message-ID: According to the MSDS sheet at the web address provided by Cindy Dubois, this solution contains water, sodium acetate, zinc chloride, phenol and citric acid - concentrations not disclosed; pH not stated. Another MSDS for ultrum at http://siri.org/msds/f2/bzr/bzrjb.html lists the ingredients as 1,5-pentanedial (that's glutaraldehyde) 3%, zinc sulphate.7H2O 1%, "carboxy hydroxide" (=??!, but 0.04%) and "buffers" 2.0%. Solutions containing glutaraldehyde and phenol are used as disinfectants; all kinds of additives, not the ones listed for ultrum, are included to increase shelf life and reduce the corrosive effect on metals (see eg Schattner 1978 US Patent 410301). Phenol accelerates protein cross-linking by formaldehyde, and phenol-formaldehyde fixatives were sometimes used in the late 1980s to early 1990s (see Hopwood et al 1989 Histochem. J. 21:228-234). This mixture never became popular. A Scopus search shows only 6 articles citing the original publication. Glutaraldehyde fixation can be bad news for light microscopy because it leaves all parts of the tissue bristling with free aldehyde groups. These can bind some dyes, are Schiff-positive and can bind proteins such as antibodies. Glutaraldehyde also induces fluorescence (not, strictly speaking autofluorescence, but just as unwanted). There are various clever ways of overcoming these undesirable actions of glutaraldehyde (eg Kasten & Lala 1975 Stain Technol. 50: 197-201; Tagliaferro et al 1997 J. Neurosci. Methods 77(2):191-197). There is no shortage of stable fixative mixtures with known composition and ingredients whose actions on tissues have been quite thoroughly studied, and which don't corrode metals. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Cindy DuBois Date: Wednesday, March 3, 2010 12:17 Subject: [Histonet] Ultram Fixative To: histonet@lists.utsouthwestern.edu > "Ultram" Fixative is actually Ultrum Fixative and is available thru > American Mastertech: > > http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=FXULTGAL > > Cindy Dubois > > > > Message: 20 > Date: Tue, 2 Mar 2010 20:08:23 -0500 > From: Robert Richmond > Subject: [Histonet] Re: Ultram fixative > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Richard Cartun asks >>We have a clinic that is fixing tissue in > "Ultram". I have never heard of it. Can someone educate me about > "Ultram"?<< > > I can't Google any evidence for an Ultram fixative. Ultram is a trade > name for tramadol, an opioid analgesic. > > Either somebody is confused, or you've got somebody involved in > dubious drug transactions. Can anybody in the clinic shed any > light on > the matter? > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stevenk <@t> med.usyd.edu.au Wed Mar 3 14:42:59 2010 From: stevenk <@t> med.usyd.edu.au (Stephen Kum Jew) Date: Wed Mar 3 14:44:05 2010 Subject: [Histonet] Re: paraffin processing times for brain In-Reply-To: <20100303180329.4604BD246F49@smtp.med.usyd.edu.au> Message-ID: Hi all I was wondering if anyone can send me please the optimal paraffin processing times for brain autopsy tissue. Currently the blocks have cut and stained well but 4-5mm thick tissue has expanded (bulged out) following cutting as if they had absorbed water. Solutions been all changed. thanks Stephen |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9036 9027 |Fax:+61 2 9351 3429 From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Mar 3 14:52:34 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Mar 3 14:54:15 2010 Subject: [Histonet] RE: paraffin processing times for brain In-Reply-To: References: <20100303180329.4604BD246F49@smtp.med.usyd.edu.au>, Message-ID: we used to do one hour in each step formalin through to paraffin (2 steps of paraffin). But 4 to 5mm is a little on the thick side. We used to try for 3 mm. But didn't always get it that way. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Kum Jew [stevenk@med.usyd.edu.au] Sent: Wednesday, March 03, 2010 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: paraffin processing times for brain Hi all I was wondering if anyone can send me please the optimal paraffin processing times for brain autopsy tissue. Currently the blocks have cut and stained well but 4-5mm thick tissue has expanded (bulged out) following cutting as if they had absorbed water. Solutions been all changed. thanks Stephen |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9036 9027 |Fax:+61 2 9351 3429 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lori.Disher <@t> HCAhealthcare.com Wed Mar 3 15:30:31 2010 From: Lori.Disher <@t> HCAhealthcare.com (Disher Lori) Date: Wed Mar 3 15:30:38 2010 Subject: [Histonet] Meditech and Computer Requisitions vs Paper Req's Message-ID: <778DD853CF606049A37FC2059C8BA07A62A3B0BE5C@FWDCWPMSGCMS04.hca.corpad.net> Our hospital uses Meditech and in the near future we are going to go to CPOE (computerized physician order entry). We currently are the only dept that uses paper requisitions for Surgical Specimens and Cytology. For those of you out there that has gone through this transition, can you share any issues you have had? Thank you. Lori A Disher Lead Histology Tech Fawcett Memorial Hospital 21298 Olean Blvd, Port Charlotte, FL 33952 phone 941-627-6128 fax 941-764-7071 lori.disher@hcahealthcare.com From Todd.Krueger <@t> bsci.com Wed Mar 3 16:07:05 2010 From: Todd.Krueger <@t> bsci.com (Krueger, Todd) Date: Wed Mar 3 16:07:16 2010 Subject: [Histonet] Butyl alcohol Message-ID: <669973B45D49DA43B0FA9AC9609CBF54042376C5@MAPMAIL01.bsci.bossci.com> Has anyone tried using butyl alcohol as a dehydrating and clearing agent on there tissues? What are some pros and cons people have experienced with using Butyl alcohol? Todd Krueger HTL(ASCP)CM Boston Scientific 2 Scimed Place, P121 Osseo, MN 55311 Phone: 763-694-5709 Fax: 763-694-5505 e-mail: todd.krueger@bsci.com From rsrichmond <@t> gmail.com Wed Mar 3 16:10:02 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Mar 3 16:10:07 2010 Subject: [Histonet] Re: Ultrum II ("Ultram") fixative Message-ID: Richard Cartun asked about "Ultram" fixative, which Cindy Dubois informs us is actually Ultrum fixative. According to its MSDS (dated 2003), Ultrum II tissue fixative contains glutaraldehyde, phenol, zinc chloride, sodium acetate, citric acid, and water. It is offered by American Master*Tech Scientific, Inc. in Lodi CA. http://www.americanmastertech.com/histologysupply.htm They also supply genuine B5 fixative, Bouin's fixative, and Carnoy's fixative. Some patent applications from a few years back suggest that Ultrum II was used to fix prostate biopsy specimens. Obviously glutaraldehyde and phenol both have safety and environmental problems of their own. Whether they would practically and legally support IHC would be hard to predict. And in diagnosing prostate cancer, the use of nucleoli as a criterion of malignancy requires NBF - other fixatives are likely to result in demonstrating nucleoli in benign cells. Bob Richmond Samurai Pathologist Knoxville TN From jcox90 <@t> yahoo.com Wed Mar 3 18:04:01 2010 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Wed Mar 3 18:04:05 2010 Subject: [Histonet] VIP basket for K1000 Message-ID: <558908.41754.qm@web56808.mail.re3.yahoo.com> Hi Netters!! Does anyone have an extra basket that I can borrow or purchase for the K1000 VIP? Doctor wants to start processing but basket wasn't included in processor. It's the small one. Thanks!!! I am in AZ if anyone here has one, I can come pick up.. Thanks!! Jill Jill Cox HT (ASCP) From CIngles <@t> uwhealth.org Wed Mar 3 21:08:44 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Mar 3 21:10:34 2010 Subject: [Histonet] Alcohol Source? References: <4D14F0FC9316DD41972D5F03C070908B02E46EE8@nmdamailsvr.nmda.ad.nmsu.edu> <8F48E360671943E38662E52001109611@JoePC> Message-ID: I vote for the scotch! Claire ________________________________ depends. A fine Scotch whiskey may be aged in old oak casks, while a delicate Tequila may not be From CIngles <@t> uwhealth.org Wed Mar 3 21:11:05 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Mar 3 21:13:05 2010 Subject: [Histonet] Re: bone marrow fixative References: Message-ID: Ah, in a perfect world! (which would be down right boring, thanks) Claire Neutral buffered formalin requires time for fixation - clots need to be cut up as soon as possible after they're received, and biopsy specimens really ought to fix overnight before decalcification and processing. Communication with oncologists is essential (and rarely achievable). Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Mar 3 23:45:34 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Mar 3 23:45:38 2010 Subject: [Histonet] Butyl alcohol Message-ID: Dear Todd Krueger, There are three isomeric butyl alcohols (primary, secondary and tertiary) with different physical properties. and different uses in histology for primary and tertiary. I have used tertiary butyl alcohol (= t-butanol = 2-methylpropan-2-ol) a few times for processing into paraffin. It mixes with with both water and wax. It boils at 83C so it's slightly less of a fire hazard than ethanol. It doesn't form explosive peroxides with long storage, which makes it safer than dioxane and tetrahydrofuran, two other "universal solvents" that have been used for combined dehydration and clearing. t-Butanol doesn't have an offensive odour, but it is solid below 25C, which is inconvenient, and it's quite a bit more expensive than more commonly used solvents such as ethanol, isopropanol and xylene. t-Butanol was introduced as a combined dehydration and clearing agent by Larbaud (1921) Compt. Rend. Acad. Sci. 172:1317-1319. It is used more in plant than in animal histology. Primary butyl alcohol (n-butanol) is liquid at ordinary temperatures. It is only partly miscible with water, but miscible with ethanol-water mixtures and with paraffin. It has been recommended for transitioning to wax in procedures claimed to reduce hardening of wood (Zirkle 1930, Science 71:103-104) and insect specimens (Stiles 1934, Stain Technol. 9:97-100). Freeze-substitution into n-butanol can be followed by paraffin embedding because this alcohol is a liquid from -90 to +117C. I haven't tried any of these methods. Another use of n-butanol is in dehydration of sections stained with dyes that are easily extracted by water or water-ethanol mixtures. I have lots of experience in this area. A major disadvantage of n-butanol, for any application, is its vapour. It doesn't smell nasty but it makes you cough. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Krueger, Todd" Date: Wednesday, March 3, 2010 17:08 Subject: [Histonet] Butyl alcohol To: histonet@lists.utsouthwestern.edu > Has anyone tried using butyl alcohol as a dehydrating and > clearing agent > on there tissues? What are some pros and cons people have experienced > with using Butyl alcohol? > > Todd Krueger > > HTL(ASCP)CM > > Boston Scientific > > 2 Scimed Place, P121 > > Osseo, MN 55311 > > Phone: 763-694-5709 > > Fax: 763-694-5505 > > e-mail: todd.krueger@bsci.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Thu Mar 4 09:11:37 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Mar 4 09:11:42 2010 Subject: [Histonet] Histo Supervisor Needed In Florida Message-ID: Allied Search Partners is currently accepting resumes for a Histology Supervisor in Polk County (Auburndale, FL area). Position Title: Histology Supervisor Duties include but are not limited to: - Provide comprehensive management of the Histology section to ensure delivery of quality laboratory services, supervising a staff of 4. - Is responsible for performance of quality specimen processing, embedding, cutting, routine and special staining, frozen sectioning, and mounting of surgical and autopsy materials - Performs evaluations and competency assessments of staff. - Maintains compliance with regulatory agencies. - Manages functions through us of pathology computer system. Requirements: 1. BS degree and completion of a Histology training program. 2. Licensed by the State of Florida as a Histology Supervisor or Technologist with supervisor license to be acquired within 90 days of hire date. 3. 5 years of progressively more responsible experience in a Histology department. 4. Management experience necessary to implement policies, procedures and develop operating budgets for section. 5. National certification. To apply for this position, please send resume to alyssa@alliedsearchpartners.com . No resume will be submitted to client without speaking to you first for a phone interview. All resumes kept confidential. From gagnone <@t> KGH.KARI.NET Thu Mar 4 09:48:16 2010 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Thu Mar 4 09:48:24 2010 Subject: [Histonet] Negative controls Message-ID: Hi Anita, We are currently examining the negative control issue in our laboratory. We also have Ventanas. We currently use a normal goat serum as our "non-immune" serum. We are considering changing our SOP's to use the Ventana Neg Control Rabbit and Neg Control Mouse in dispensers. Linda's suggestion of Biocare's Universal Negative Control Serum is intriguing. It would seem regressive to go back to the days of delineating mouse/rabbit antisera, using separate dispensers for each, and a universal negative seems to be the way to go, especially when using universal DAB kits. Using such a reagent would mean it could be applied to each case's negative control slide. Linda or others, would such a negative control serum protocol include your most aggressive pretreatment? Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From gu.lang <@t> gmx.at Thu Mar 4 09:48:37 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Mar 4 09:48:45 2010 Subject: AW: [Histonet] Re: paraffin processing times for brain In-Reply-To: References: <20100303180329.4604BD246F49@smtp.med.usyd.edu.au> Message-ID: Stephen, we doubled the routine protocol for braintissue from 13 to 24 hour. We stayed at one hour for the formalin steps, because the tissue is fixed for several days before processing. This protocol is also used for prostate big cassettes. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Stephen Kum Jew Gesendet: Mittwoch, 03. M?rz 2010 21:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: paraffin processing times for brain Hi all I was wondering if anyone can send me please the optimal paraffin processing times for brain autopsy tissue. Currently the blocks have cut and stained well but 4-5mm thick tissue has expanded (bulged out) following cutting as if they had absorbed water. Solutions been all changed. thanks Stephen |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9036 9027 |Fax:+61 2 9351 3429 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Thu Mar 4 09:52:28 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Mar 4 09:54:10 2010 Subject: [Histonet] Negative controls In-Reply-To: References: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B64617F@chi2k3ms01.columbuschildrens.net> We too use the Universal negative from Biocare, and it has passed through 2 CAP inspections so no problem on that front. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Thursday, March 04, 2010 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hi Anita, We are currently examining the negative control issue in our laboratory. We also have Ventanas. We currently use a normal goat serum as our "non-immune" serum. We are considering changing our SOP's to use the Ventana Neg Control Rabbit and Neg Control Mouse in dispensers. Linda's suggestion of Biocare's Universal Negative Control Serum is intriguing. It would seem regressive to go back to the days of delineating mouse/rabbit antisera, using separate dispensers for each, and a universal negative seems to be the way to go, especially when using universal DAB kits. Using such a reagent would mean it could be applied to each case's negative control slide. Linda or others, would such a negative control serum protocol include your most aggressive pretreatment? Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. 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From SDrew <@t> uwhealth.org Thu Mar 4 10:01:59 2010 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Thu Mar 4 10:03:03 2010 Subject: [Histonet] Negative controls In-Reply-To: Message-ID: <738A7878143FF74BB77436E255743C1A010004C7@UWHC-MAIL03.uwhis.hosp.wisc.edu> Yes, Linda and I select the harshest protocol in the list of antibodies we're running per case-we figure that the harshest will produce the biggest problems with background, etc. Sally Ann Drew, MT(ASCP) IHC/ISH Clinical & Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison, WI 53792 (608)265-6596 Fax:(608)262-7174 Hi Anita, We are currently examining the negative control issue in our laboratory. We also have Ventanas. We currently use a normal goat serum as our "non-immune" serum. We are considering changing our SOP's to use the Ventana Neg Control Rabbit and Neg Control Mouse in dispensers. Linda's suggestion of Biocare's Universal Negative Control Serum is intriguing. It would seem regressive to go back to the days of delineating mouse/rabbit antisera, using separate dispensers for each, and a universal negative seems to be the way to go, especially when using universal DAB kits. Using such a reagent would mean it could be applied to each case's negative control slide. Linda or others, would such a negative control serum protocol include your most aggressive pretreatment? Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From W.E.J.Hoekert <@t> olvg.nl Thu Mar 4 09:52:01 2010 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Thu Mar 4 10:03:22 2010 Subject: [Histonet] How to do an EBER on cytological material? Message-ID: <1190CB05C44B13409483514729C2FC360C0AA6@PAIT42.olvg.nl> Dear histonetters, I was wondering if it is possible to do an EBER ISH on a cell-smear. What pretreatment should I use? 1: Fix the cells in formalin (let's say overnight, or else one or two hours), and than use the proteinase K digestion step. 2: Or maybe it is enough to fix the cells in cold acetone, and skip the proteinase K step? Or will the RNA leak out of the cells? Does anybody has experience whit this? We only have two slides left, and we don't have (cytological) control material, only FFPE control material. Also, I think that we should bake the slides prior to the asessment because we might lose a lot of cells during the (stringent) washing steps. Maybe it is not possible at all because the RNA is broken down allready (the cell smears were taken 3 days ago, they were stored at room temperature). I hope you folks can help me out. Willem Hoekert OLVG, The Netherlands Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From kfineout <@t> hotmail.com Thu Mar 4 12:50:18 2010 From: kfineout <@t> hotmail.com (Kelly Larson) Date: Thu Mar 4 12:50:23 2010 Subject: [Histonet] Bone Marrow Fixative Message-ID: I am using Fix-All from Surgipath for bone marrow fixation. I empty the syringe into the fixative as soon as it is handed to me (no messing around with clots). I fix for 1-2 hours in Fix-All then transfer to a screen cassette and our normal processing with 10%NBF. The pathologist is very happy with the results. Kelly Larson, HT(ASCP) Pathology Services of West MI EMAILING FOR THE GREATER GOOD Join me From Joyce.Cline <@t> wchsys.org Thu Mar 4 13:06:58 2010 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Thu Mar 4 13:16:36 2010 Subject: [Histonet] RE: Meditech and Computer Requisitions vs Paper Req's In-Reply-To: <778DD853CF606049A37FC2059C8BA07A62A3B0BE5C@FWDCWPMSGCMS04.hca.corpad.net> References: <778DD853CF606049A37FC2059C8BA07A62A3B0BE5C@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: We have Meditech but have never used order entry. Our IS determined with the mistakes the floors make in entering they would not include us in order entry. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Disher Lori [Lori.Disher@HCAhealthcare.com] Sent: Wednesday, March 03, 2010 4:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech and Computer Requisitions vs Paper Req's Our hospital uses Meditech and in the near future we are going to go to CPOE (computerized physician order entry). We currently are the only dept that uses paper requisitions for Surgical Specimens and Cytology. For those of you out there that has gone through this transition, can you share any issues you have had? Thank you. Lori A Disher Lead Histology Tech Fawcett Memorial Hospital 21298 Olean Blvd, Port Charlotte, FL 33952 phone 941-627-6128 fax 941-764-7071 lori.disher@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Allison_Scott <@t> hchd.tmc.edu Thu Mar 4 13:46:28 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Mar 4 13:46:34 2010 Subject: [Histonet] Dual Locations Message-ID: <1872B4A455B7974391609AD8034C79FC8BD6E0@LBEXCH01.hchd.local> Hello to everyone in histoland. We are currently building a new medical tower next to the hospital. Our histology lab will be moving there, but the existing frozen section area will remain in the main hospital. How are those with multiple sites dealing with staffing and specimen and slide flow. This project will not be completed until 2012. I am currenltly working with the architects on making sure that all of our equipment and room for growth needs are going to be met. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From gagnone <@t> KGH.KARI.NET Thu Mar 4 13:50:11 2010 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Thu Mar 4 13:50:18 2010 Subject: [Histonet] Negative Controls...thanks Message-ID: Thanks to all of you for your suggestions. We will be putting the Biocare Universal Negative Control Serum through a trial. Once again, Histonet proves it's not only invaluable, and populated by helpful, knowledge and experienced histotechs, it's also extremely expedient! Thanks, Eric From fairbairnp <@t> neurosurg.ucsf.edu Thu Mar 4 13:58:31 2010 From: fairbairnp <@t> neurosurg.ucsf.edu (Fairbairn, Patricia) Date: Thu Mar 4 13:59:24 2010 Subject: [Histonet] Cryostat better than others? Message-ID: Hi All, Are there any cryostat makes that seem to be more reliable than others? We have not been having much luck with our particular Microms after moving them cross-country, getting recalled, catching on fire, corroding, etc. While one machine serves as an excellent calendar stand (with padded resting pads and everything!) and our other one just went down at the ripe age of 2, I would rather purchase machines that work without costing us so much in repairs. ...or are we just jinxed? Also, how long do cryostats tend to last? What do you think of service contracts? I keep hearing that they are pretty much worthless and that the cryostat "should" be fine if it's served once a year. Thank you! PJ Fairbairn Staff Research Associate Department of Neurological Surgery Brain and Spinal Injury Center University of California at San Francisco fairbairnp@neurosurg.ucsf.edu From derek.papalegis <@t> tufts.edu Thu Mar 4 14:00:45 2010 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Thu Mar 4 14:00:49 2010 Subject: [Histonet] glass coverslipper Message-ID: <4B90116D.2090006@tufts.edu> Hi Everyone, I am looking for a glass coverslipper and was wondering what people recommend. I am in a small research lab so having a small footprint is very important. It doesn't have to be very fast but I need something dependable that will give consistent results. Thanks, Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 From Joyce.Cline <@t> wchsys.org Thu Mar 4 13:59:59 2010 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Thu Mar 4 14:08:00 2010 Subject: [Histonet] RE: Dual Locations In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD6E0@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC8BD6E0@LBEXCH01.hchd.local> Message-ID: We are building a new hospital also. Currently the old hospital grossing is done there and the cassettes are shipped over to our lab site. We use AP assistants (high school diploma) to accession, assist the path and cut & stain the frozen sections. (Actually our AP assistants were formally Phlebs) The new hospital is going up where we are currently and the AP Assistants will go over there to cut & stain the frozens from the OR. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D [Allison_Scott@hchd.tmc.edu] Sent: Thursday, March 04, 2010 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dual Locations Hello to everyone in histoland. We are currently building a new medical tower next to the hospital. Our histology lab will be moving there, but the existing frozen section area will remain in the main hospital. How are those with multiple sites dealing with staffing and specimen and slide flow. This project will not be completed until 2012. I am currenltly working with the architects on making sure that all of our equipment and room for growth needs are going to be met. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Maria.Katleba <@t> stjoe.org Thu Mar 4 14:09:16 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Mar 4 14:09:45 2010 Subject: [Histonet] RE: Meditech and Computer Requisitions vs Paper Req's In-Reply-To: References: <778DD853CF606049A37FC2059C8BA07A62A3B0BE5C@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: Meditech is an awful program for Pathology. I loved using Pathologix (the Software company... not the Reference lab that is stealing histology jobs from hospitals). Even Co-path is acceptable. Meditech does not lend itself to Pathology as it does to clinical lab. It is cumbersome and not user friendly... no matter how well you are at using computers... and as we all know, Pa's and Pathologists like having a requisition to make notes on and refer back to. We luckily are still on downtime forms and we will not be moving into using Meditech for Doctors' orders..... Just my humble opinion! Maria -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, March 04, 2010 11:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Meditech and Computer Requisitions vs Paper Req's We have Meditech but have never used order entry. Our IS determined with the mistakes the floors make in entering they would not include us in order entry. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Disher Lori [Lori.Disher@HCAhealthcare.com] Sent: Wednesday, March 03, 2010 4:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech and Computer Requisitions vs Paper Req's Our hospital uses Meditech and in the near future we are going to go to CPOE (computerized physician order entry). We currently are the only dept that uses paper requisitions for Surgical Specimens and Cytology. For those of you out there that has gone through this transition, can you share any issues you have had? Thank you. Lori A Disher Lead Histology Tech Fawcett Memorial Hospital 21298 Olean Blvd, Port Charlotte, FL 33952 phone 941-627-6128 fax 941-764-7071 lori.disher@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From trathborne <@t> somerset-healthcare.com Thu Mar 4 14:13:00 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Mar 4 14:13:05 2010 Subject: [Histonet] Cryostat better than others? In-Reply-To: Message-ID: We currently have a Leica and a Jung. They are serviced twice a year. Prior to these, we had two Tissue Teks that were donated to us. My best guess is that they were about 20 years old when they were replaced. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Fairbairn, Patricia Sent: Thursday, March 04, 2010 2:59 PM To: Histonet Subject: [Histonet] Cryostat better than others? Hi All, Are there any cryostat makes that seem to be more reliable than others? We have not been having much luck with our particular Microms after moving them cross-country, getting recalled, catching on fire, corroding, etc. While one machine serves as an excellent calendar stand (with padded resting pads and everything!) and our other one just went down at the ripe age of 2, I would rather purchase machines that work without costing us so much in repairs. ...or are we just jinxed? Also, how long do cryostats tend to last? What do you think of service contracts? I keep hearing that they are pretty much worthless and that the cryostat "should" be fine if it's served once a year. Thank you! PJ Fairbairn Staff Research Associate Department of Neurological Surgery Brain and Spinal Injury Center University of California at San Francisco fairbairnp@neurosurg.ucsf.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From trathborne <@t> somerset-healthcare.com Thu Mar 4 14:13:51 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Mar 4 14:13:57 2010 Subject: [Histonet] glass coverslipper In-Reply-To: <4B90116D.2090006@tufts.edu> Message-ID: Try the Leica CV5030. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Derek Papalegis Sent: Thursday, March 04, 2010 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] glass coverslipper Hi Everyone, I am looking for a glass coverslipper and was wondering what people recommend. I am in a small research lab so having a small footprint is very important. It doesn't have to be very fast but I need something dependable that will give consistent results. Thanks, Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From pbaldwin <@t> theadvisoryboardprogram.com Thu Mar 4 14:14:53 2010 From: pbaldwin <@t> theadvisoryboardprogram.com (Peter Baldwin) Date: Thu Mar 4 14:15:20 2010 Subject: [Histonet] Fire in the lab Message-ID: <25AC51D9A181474E8A845F77077AFF1E29DFE19C@winxbeus22.exchange.xchg> Very sorry to hear about this incident and thank goodness the tech didn't get injured AND used quick-thinking to grab the extinguisher and put out the fire.? This incident exemplifies the (hidden) dangers inherent in the operation of labs that, often are overlooked ("we haven't had a fire in 20 years!").? Oftentimes, lab personnel are unaware of the powerful impact of flammable liquids, because they are used every day with no apparent adverse effects - until it is too late! Peter Peter G. Baldwin Director of Sales, Marketing & Business Development pbaldwin@MicronEnvironmental.com Micron Environmental Industries, Inc. Green Chemistry for LifeSM www.MicronEnvironmental.com ?? 1221 Cameron Street Alexandria, VA 22314 703-548-2776 703-548-7988/Fax From 41dmb41 <@t> gmail.com Thu Mar 4 14:34:02 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Thu Mar 4 14:34:27 2010 Subject: [Histonet] glass coverslipper In-Reply-To: <4B90116D.2090006@tufts.edu> References: <4B90116D.2090006@tufts.edu> Message-ID: We use the Tissue-Tek Glas with great results. Knowing how Sakura loves to just relabel Leica equipment, it might be the same as the CV5030, but I'm not sure. Either way, I've been very impressed with the Tissue-Tek machine. Drew On Thu, Mar 4, 2010 at 15:00, Derek Papalegis wrote: > Hi Everyone, > I am looking for a glass coverslipper and was wondering what people > recommend. I am in a small research lab so having a small footprint is very > important. It doesn't have to be very fast but I need something dependable > that will give consistent results. > > Thanks, > Derek > > -- > Derek Papalegis HT (ASCP) > Senior Histology Technologist > Division of Laboratory Animal Medicine > Tufts University 136 Harrison Avenue > Boston, MA 02111 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dchihc <@t> yahoo.com Thu Mar 4 14:35:28 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Thu Mar 4 14:35:34 2010 Subject: [Histonet] Full Time Day Shift DCH Regional Medical Center Tuscaloosa, Alabama Message-ID: <947438.80446.qm@web43503.mail.sp1.yahoo.com> At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday.?Must be ASCP registered HT or HTL (or eligible...must become registered within 18 months of hire date). Lab Automation includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura?XPress 50 rapid tissue processor, Leica cryostats. Duties include embedding, microtomy, ihc and special stains, and frozen sections. DCH is a 500 plus bed regional medical center. We process around 12,000 to 13,000 specimens annually.? Interested candidates please contact Sherrie Faulkner?(recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From cfrmd1 <@t> gmail.com Thu Mar 4 17:51:51 2010 From: cfrmd1 <@t> gmail.com (Carlos Rodriguez, MD) Date: Thu Mar 4 17:51:56 2010 Subject: [Histonet] IHC cost-effectiveness Message-ID: <65c42edc1003041551x141eeffdv919b1e601d3e009d@mail.gmail.com> Hi everyone These questions are primarily for those people who currently work in, or have experience in private practices (derm, GI) that have in-house path labs. For IHC, how cost-effective is it to do immunos in-house *using kits*? No immunostainer would be available. I imagine it's substantially more expensive than doing H&E and special stains. Also, how many slides can a typical single kit be used for, and how time-consuming is the actual process of manual IHC staining? Lastly, do practices run into problems sending cases out to larger labs for the TC of immunostains, and then doing the read (PC) themselves in-house? Do insurers or CMS disallows this practice or not reimburse 2 separate billers for the same case? Thanks very much. Carlos Rodriguez, MD From andreahooper <@t> rocketmail.com Thu Mar 4 23:54:17 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Thu Mar 4 23:54:20 2010 Subject: [Histonet] IF staining on peritoneal macrophages In-Reply-To: <1b2831cd1003031218g4bbf2446kef5daebdfcd260d7@mail.gmail.com> Message-ID: <527548.71315.qm@web113105.mail.gq1.yahoo.com> Dear Mauricio, ? The two antibodies you chose were NOT cross adsorbed against mouse. This is critical for successful staining of mouse tissue with rat antibodies. These are the two alternate catalog #s of the ones I would recommend if FITC is your thing: ? 712-095-153?(whole IgG FITC conjugated) 712-096-153??(Fab'2 FITC conjugated) ? Although the important part to reduce background and increase sensitivity is the antibody itself, the choice of fluorophore can also play a huge role in your success. There are now many much better fluorophores than FITC and I suggest you play around with some of those. ? Firstly are you fixed on using the "green channel" (ie FITC) vs the "red channel" (ie rhoadmine)? I personally like CY3 (red)?the best for brightness of the Jackson IR fluorophores. CY2 is also great. They also have a new series called DyLights which are reported to be great. I have played with them a little bit with some success and some failures. ? Ok, so assuming you want to stick with the green channel (I suggest you use CY2 then)?these are the two simple?choices for you? (the second one is a F(ab')2 and one is a whole IgG - 99/100 the whole IgG will be fine for you and is what I use for staining hematopoietic tissue ...): ? 712-225-153?(whole IgG CY2 conjugated) 712-226-153?(Fab'2 CY2 conjugated) ? Another option for boosting signal is to use a biotinylated antibody and then use streptavidin conjugated to your fluorophore of choice. Then if you want to use the Alexa488s you can get streptavidin-Alexa488 from Invitrogen. ? 712-065-153?(whole IgG biotinylated) ? In my lab, I use both the CY2/CY3 conjugates as well as the biotin conjugates routinely. I rarely if ever use the F(ab')2 antibodies, it's normally overkill. ? Let me know if you need anything else, Andrea ? ? --- On Wed, 3/3/10, Mauricio Avigdor wrote: From: Mauricio Avigdor Subject: Re: [Histonet] IF staining on peritoneal macrophages To: "Andrea T. Hooper" , histonet@lists.utsouthwestern.edu Date: Wednesday, March 3, 2010, 8:18 PM Hi Andrea, ? Jackson lists a couple of FITC-conjugated donkey anti-rat secondaries: 712-095-150?- Whole Donkey Anti-Rat IgG (H+L). ? 712-096-150 - F(ab')2 fragment Donkey Anti-Rat?IgG (H+L). ? Do you recognize which is the one you have ben using. ? I have been using Serotec's STAR80F antibody. They suggest using 1:10 Normal Mouse Serum in PBS to make the necessary dilutions. Is this something you do with the Jackson antibodies as well? I've never heard of this technique before. From andreahooper <@t> rocketmail.com Fri Mar 5 00:05:38 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Fri Mar 5 00:05:42 2010 Subject: [Histonet] IF staining on peritoneal macrophages In-Reply-To: <1b2831cd1003031218g4bbf2446kef5daebdfcd260d7@mail.gmail.com> Message-ID: <344807.58798.qm@web113109.mail.gq1.yahoo.com> Sorry for the second email, I didn't address all your questions ... ? The antibody I use from Serotec is?MCA497, works very well and many people on Histonet have claimed the same over the years. ? I am not familiar with the secondary you use from Serotec. It says it's goat anti-rat IgG, mouse adsorbed. So it should be ok? But again FITC really isn't a great fluorophore and I tend not to use anything except Jackson IR reagents. I don't understand why they would suggest to use mouse serum, especially since it's mouse adsorbed?? Unless you are trying to block Fc receptors - again, probably overkill. I have stained mouse hematopoietic tissue for years with rat antibodies and secondaries from?Jackson?and have never had this problem.?I think this mouse serum step will just add background. Don't do it. Usually one is supposed to dilute their secondaries in the serum that the secondary is made in. Therefore in your case, I would use goat IgG. Block in your favorite blocking recipe ... For the antibodies I recommended to you, I use 10% donkey serum with?5% BSA (make sure it's gamma globulin free) in buffer with some detergent. Incubate primaries in 0.2X concentration of your block. ? If you want to share your protocol, maybe I can give you some further tips? ? Let me know if I can help further, Andrea ? --- On Wed, 3/3/10, Mauricio Avigdor wrote: From: Mauricio Avigdor Subject: Re: [Histonet] IF staining on peritoneal macrophages To: "Andrea T. Hooper" , histonet@lists.utsouthwestern.edu Date: Wednesday, March 3, 2010, 8:18 PM Hi Andrea, ? Jackson lists a couple of FITC-conjugated donkey anti-rat secondaries: 712-095-150?- Whole Donkey Anti-Rat IgG (H+L). ? 712-096-150 - F(ab')2 fragment Donkey Anti-Rat?IgG (H+L). ? Do you recognize which is the one you have ben using. ? I have been using Serotec's STAR80F antibody. They suggest using 1:10 Normal Mouse Serum in PBS to make the necessary dilutions. Is this something you do with the Jackson antibodies as well? I've never heard of this technique before. From ree3 <@t> leicester.ac.uk Fri Mar 5 03:14:30 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Fri Mar 5 03:15:03 2010 Subject: [Histonet] Negative controls In-Reply-To: <738A7878143FF74BB77436E255743C1A010004C7@UWHC-MAIL03.uwhis.hosp.wisc.edu> References: <738A7878143FF74BB77436E255743C1A010004C7@UWHC-MAIL03.uwhis.hosp.wisc.edu> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8CFFB6C0C@EXC-MBX3.cfs.le.ac.uk> So if one is doing an immunostaining staining run with say, 10 different primary antibodies, at different dilutions, would you have the the same number of different dilutions of the Biocare universal control, or just have the one at the least dilute, say 1/10??, thanks. Richard Edwards Leicester University....U.K.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Sally A Sent: 04 March 2010 16:02 To: Histonet Subject: RE: [Histonet] Negative controls Yes, Linda and I select the harshest protocol in the list of antibodies we're running per case-we figure that the harshest will produce the biggest problems with background, etc. Sally Ann Drew, MT(ASCP) IHC/ISH Clinical & Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison, WI 53792 (608)265-6596 Fax:(608)262-7174 Hi Anita, We are currently examining the negative control issue in our laboratory. We also have Ventanas. We currently use a normal goat serum as our "non-immune" serum. We are considering changing our SOP's to use the Ventana Neg Control Rabbit and Neg Control Mouse in dispensers. Linda's suggestion of Biocare's Universal Negative Control Serum is intriguing. It would seem regressive to go back to the days of delineating mouse/rabbit antisera, using separate dispensers for each, and a universal negative seems to be the way to go, especially when using universal DAB kits. Using such a reagent would mean it could be applied to each case's negative control slide. Linda or others, would such a negative control serum protocol include your most aggressive pretreatment? Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NSEARCY <@t> swmail.sw.org Fri Mar 5 06:44:58 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Mar 5 06:45:08 2010 Subject: [Histonet] Arcturas Micro Laser Capture Message-ID: <4B90A869.5D38.00EF.0@swmail.sw.org> Anyone in Texas using this instrument? I have called local rep for information, as well as retraining information, but have yet to hear from them. Anyone else using? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From adpathlab2 <@t> gmail.com Fri Mar 5 07:32:06 2010 From: adpathlab2 <@t> gmail.com (Affiliated Dermatology Histology Lab) Date: Fri Mar 5 07:32:13 2010 Subject: [Histonet] ihc stainer Message-ID: We are a small lab with a low volume and we are thinking about beginning to do our own ihc's. Can anyone recommend an ihc stainer that would be suitable for a small lab? From trathborne <@t> somerset-healthcare.com Fri Mar 5 08:15:27 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Mar 5 08:15:33 2010 Subject: [Histonet] ihc stainer In-Reply-To: Message-ID: You might want to check out Celerus. http://www.celerusdiagnostics.com/about -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Affiliated Dermatology Histology Lab Sent: Friday, March 05, 2010 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ihc stainer We are a small lab with a low volume and we are thinking about beginning to do our own ihc's. Can anyone recommend an ihc stainer that would be suitable for a small lab? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From alyssa <@t> alliedsearchpartners.com Fri Mar 5 08:21:57 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Mar 5 08:22:01 2010 Subject: [Histonet] HT/HTL Needed In NJ Job Leads Into Supervisor Role (Near NYC) Message-ID: Allied Search Partners is currently accepting resumes for a histotechnician/histotechnologist that can lead into a *supervisory role*. Ideally we are looking for a candidate with at least 5 years experience and with flow cytometry experience. Location: Clifton, NJ area (near the border of NY and just north of Jersey City, NJ) Local candidates only please. *Essential Functions and Duties* * * Must have IHC and Cyto prep experience. Must be able to work independently under minimal supervision, maintain laboratory supplies, equipment, and QC/QA records Must be knowledgeable with basic computer skills and pathology software, and participate in improvement of laboratory procedures. The person should be reliable with great inter-personal communication skills, and willing to coordinate with other departmental staff. Shift: Hours required for this position are 6 am ? 2 pm, Tuesday through Saturday. Full Time Direct Hire (Permanent) *Requirements* Bachelor?s degree preferred but not required HT (ASCP) preferred The Bachelor degree may be waived if the candidate has extensive experience (>5 years) or is a certified HTL (ASCP). At least 3 years of histotechnology experience including routine histology, cytology preparation, immunohistochemistry staining (automated), special stain (manual, for bone marrow and others). To apply for this position please submit resume to alyssa@alliedsearchpartners.com for initial prescreening. No resume will be submitted to client until we speak to you for a phone interview. All resumes kept confidential. From alyssa <@t> alliedsearchpartners.com Fri Mar 5 09:14:11 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Mar 5 09:14:17 2010 Subject: [Histonet] Histotech II Needed In Tavares, FL Message-ID: Allied Search Partners is currently accepting resumes for a histotechnician/histotechnologist II. Location: Tavares, FL area *Essential Functions and Duties* * * Must be able to work independently under minimal supervision, maintain laboratory supplies, equipment, and QC/QA records The person should be reliable with great inter-personal communication skills, and willing to coordinate with other departmental staff. Shift: Hours required for this position are 8am-4:30pm Monday-Friday, with alternating Saturdays. Full Time, Permanent (Direct Hire). Nonexempt. * * *Requirements* Bachelor?s degree preferred but not required HT (ASCP) required The Bachelor degree may be waived if the candidate has extensive experience (>5 years) or is a certified HTL (ASCP). Other Positions In Florida: Histology Survisor position in Polk County To apply for this position please submit resume to alyssa@alliedsearchpartners.com for initial prescreening. No resume will be submitted to client until we speak to you for a phone interview. All resumes kept confidential. From dchihc <@t> yahoo.com Fri Mar 5 12:37:29 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Fri Mar 5 12:37:34 2010 Subject: [Histonet] (no subject) Message-ID: <84454.71048.qm@web43504.mail.sp1.yahoo.com> Full Time Day Shift DCH Regional Medical Center Tuscaloosa, Alabama Thu, March 4, 2010 2:35:28 PM From: Phyllis Thaxton ?Add to Contacts To: histonet@lists.utsouthwestern.edu ________________________________ At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday.?Must be ASCP registered HT or HTL (or eligible...must become registered within 18 months of hire date). Lab Automation includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura?XPress 50 rapid tissue processor, Leica cryostats. Duties include embedding, microtomy, ihc, special stains, and frozen sections. DCH is a 500 plus bed regional medical center. We process around 12,000 to 13,000 specimens annually.? Interested candidates please contact Sherrie Faulkner?(recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From rsrichmond <@t> gmail.com Fri Mar 5 13:07:01 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Mar 5 13:07:05 2010 Subject: [Histonet] Re: Bone Marrow Fixative Message-ID: Kelly Larson, HT(ASCP) at Pathology Services of West Michigan notes: >>I am using Fix-All from Surgipath for bone marrow fixation. I empty the syringe into the fixative as soon as it is handed to me (no messing around with clots). I fix for 1-2 hours in Fix-All, then transfer to a screen cassette and our normal processing with 10% NBF. The pathologist is very happy with the results.<< Back when I was performing bone marrow biopsies, I'd empty the unclotted aspiration specimen in neutral buffered formalin. Formalin doesn't clot blood, so I'd be left with a mass of fine particles I could put in a tea bag for processing. (Tea bags now are much too politicized to use for this purpose.) I'd fix the clot (after chopping it up) and the bone biopsy specimen in Zenker's (actually Helly's) fixative. I would thus wind up with three paraffin blocks. Those were the days! (If I were doing bone marrows today, I'd use no fixative other than neutral buffered formalin.) According to the SurgiPath Web site, Fix-All contains formaldehyde, alcohol, and barium chloride, and is touted as a B-5 (mercury fixative) substitute. John Kiernan pointed out some time ago on Histonet that there is no rational purpose in putting barium in a fixative. Bob Richmond Samurai Pathologist Knoxville TN From JMacDonald <@t> mtsac.edu Fri Mar 5 12:20:49 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Mar 5 13:59:00 2010 Subject: [Histonet] California Society for Histotechnology Annual Symposium Message-ID: The CSH 34th Annual Symposium Program is now available. The meeting will be held May 14-16 at the Mission Inn in Riverside, CA. For the program go to: http://www.californiahistology.org/events.htm From rjbuesa <@t> yahoo.com Sat Mar 6 11:16:10 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 6 11:16:18 2010 Subject: [Histonet] IHC cost-effectiveness In-Reply-To: <65c42edc1003041551x141eeffdv919b1e601d3e009d@mail.gmail.com> Message-ID: <551334.28027.qm@web65707.mail.ac4.yahoo.com> Carlos: It will all depend on your volume and the variety of antigens you are trying to detect. What I would never recommend are specific kits for specific antigens, that will be a waste. You could have diluted antibodies and detection kits that could be used will all your antigens. In that way? the expense will be less. If you increase your work volume you could even go to concentrates and prepare your own dilutions. Ren? J. --- On Thu, 3/4/10, Carlos Rodriguez, MD wrote: From: Carlos Rodriguez, MD Subject: [Histonet] IHC cost-effectiveness To: histonet@lists.utsouthwestern.edu Date: Thursday, March 4, 2010, 6:51 PM Hi everyone These questions are primarily for those people who currently work in, or have experience in private practices (derm, GI) that have in-house path labs. For IHC, how cost-effective is it to do immunos in-house *using kits*? No immunostainer would be available. I imagine it's substantially more expensive than doing H&E and special stains. Also, how many slides can a typical single kit be used for, and how time-consuming is the actual process of manual IHC staining? Lastly, do practices run into problems sending cases out to larger labs for the TC of immunostains, and then doing the read (PC) themselves in-house? Do insurers or CMS disallows this practice or not reimburse 2 separate billers for the same case? Thanks very much. Carlos Rodriguez, MD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Mon Mar 8 04:29:49 2010 From: histonet.nospam <@t> vneubert.com (histonet.nospam@vneubert.com) Date: Mon Mar 8 04:29:55 2010 Subject: [Histonet] TGF beta on FFPE Message-ID: <20100308102949.AFC24C299FED@dd15630.kasserver.com> Hello Histonet, I'd like to know if anyone of you has experience with TGF beta receptor and TGF beta ligand IHC on FFPE tissue. Any input will be highly appreciated :) Thanks a lot V. Neubert From TMcNemar <@t> lmhealth.org Mon Mar 8 05:09:26 2010 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Mar 8 05:09:37 2010 Subject: [Histonet] RE: Meditech and Computer Requisitions vs Paper Req's In-Reply-To: <778DD853CF606049A37FC2059C8BA07A62A3B0BE5C@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: Our hospital has gone to Order Entry but we still use the paper reqs. We receive a copy of the EMR (electronic Medical record) with each in-house specimen. It took a little doing for nursing to get the required info in the right places but it works well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Disher Lori Sent: Wednesday, March 03, 2010 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech and Computer Requisitions vs Paper Req's Our hospital uses Meditech and in the near future we are going to go to CPOE (computerized physician order entry). We currently are the only dept that uses paper requisitions for Surgical Specimens and Cytology. For those of you out there that has gone through this transition, can you share any issues you have had? Thank you. Lori A Disher Lead Histology Tech Fawcett Memorial Hospital 21298 Olean Blvd, Port Charlotte, FL 33952 phone 941-627-6128 fax 941-764-7071 lori.disher@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4061. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From sjkitten <@t> live.com Mon Mar 8 07:38:56 2010 From: sjkitten <@t> live.com (S R) Date: Mon Mar 8 07:39:03 2010 Subject: [Histonet] Isopropanol monitring Message-ID: Good Monday Morning Everyone, I was wondering if anybody out there that is microwave processing is monitoring for Isopropanol? thanks in advanced sammy _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/ From Todd.Krueger <@t> bsci.com Mon Mar 8 09:00:24 2010 From: Todd.Krueger <@t> bsci.com (Krueger, Todd) Date: Mon Mar 8 09:00:30 2010 Subject: [Histonet] Butyl alcohol Message-ID: <669973B45D49DA43B0FA9AC9609CBF54042376CF@MAPMAIL01.bsci.bossci.com> Does anyone have a procedure for processing with N-butyl Alcohol. We need to find a procedure w/o xylene. Thanks Todd Krueger HTL(ASCP)CM Boston Scientific 2 Scimed Place, P121 Osseo, MN 55311 Phone: 763-694-5709 Fax: 763-694-5505 e-mail: todd.krueger@bsci.com From rjbuesa <@t> yahoo.com Mon Mar 8 12:48:42 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 8 12:48:45 2010 Subject: [Histonet] Isopropanol monitring In-Reply-To: Message-ID: <260431.6336.qm@web65706.mail.ac4.yahoo.com> With a TWA of 400 ppm I don't think it is really a need for monitoring 2-propanol. Ren? J. --- On Mon, 3/8/10, S R wrote: From: S R Subject: [Histonet] Isopropanol monitring To: "histo net" Date: Monday, March 8, 2010, 8:38 AM Good Monday Morning Everyone, I was wondering if anybody out there that is microwave processing is monitoring for Isopropanol? thanks in advanced sammy ??? ???????? ?????? ??? ? _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arvidsonkristen <@t> yahoo.com Mon Mar 8 13:27:41 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Mon Mar 8 13:27:46 2010 Subject: [Histonet] Embedding Forceps Message-ID: <184488.93528.qm@web65710.mail.ac4.yahoo.com> Anyone use special forceps for embedding?? I have some recent concerns that some of the forceps out there may be to pointy/sharp for delicate tissue (ie. skin).? Any insight?? From wingman320 <@t> hotmail.com Mon Mar 8 13:37:46 2010 From: wingman320 <@t> hotmail.com (Harlem Kaputnik) Date: Mon Mar 8 13:37:50 2010 Subject: [Histonet] Embedding Forceps In-Reply-To: <184488.93528.qm@web65710.mail.ac4.yahoo.com> References: <184488.93528.qm@web65710.mail.ac4.yahoo.com> Message-ID: Use the appropriate tool for the job needed... you don't want to damage the tissue. For the pointy/sharp forceps, just use more of a delicate touch (this comes with practice)... they have the blunt tipped forceps that work great for skin. > Date: Mon, 8 Mar 2010 11:27:41 -0800 > From: arvidsonkristen@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embedding Forceps > > Anyone use special forceps for embedding? I have some recent concerns that some of the forceps out there may be to pointy/sharp for delicate tissue (ie. skin). Any insight?? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/ From rjbuesa <@t> yahoo.com Mon Mar 8 13:50:31 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 8 13:50:35 2010 Subject: [Histonet] Butyl alcohol In-Reply-To: <669973B45D49DA43B0FA9AC9609CBF54042376CF@MAPMAIL01.bsci.bossci.com> Message-ID: <802041.79009.qm@web65713.mail.ac4.yahoo.com> Use 2-propanol instead. It is better and much cheaper. Ren? J.U --- On Mon, 3/8/10, Krueger, Todd wrote: From: Krueger, Todd Subject: [Histonet] Butyl alcohol To: histonet@lists.utsouthwestern.edu Date: Monday, March 8, 2010, 10:00 AM Does anyone have a procedure for processing with N-butyl Alcohol. We need to find a procedure w/o xylene. Thanks Todd Krueger HTL(ASCP)CM Boston Scientific 2 Scimed Place, P121 Osseo, MN 55311 Phone: 763-694-5709 Fax: 763-694-5505 e-mail: todd.krueger@bsci.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Mon Mar 8 13:58:13 2010 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Mon Mar 8 14:02:15 2010 Subject: [Histonet] TGF beta on FFPE Message-ID: <11D9615B89C10747B1C985966A63D7CA2CAB272B31@KCL-MAIL04.kclad.ds.kcl.ac.uk> What species, V? More info? Them TGF ligand /receptors are as difficult as FGF/FGFr, imho. respect, Carlos. From histonet.nospam <@t> vneubert.com Mon Mar 8 15:12:06 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Mon Mar 8 15:14:31 2010 Subject: [Histonet] TGF beta on FFPE In-Reply-To: <11D9615B89C10747B1C985966A63D7CA2CAB272B31@KCL-MAIL04.kclad.ds.kcl.ac.uk> References: <11D9615B89C10747B1C985966A63D7CA2CAB272B31@KCL-MAIL04.kclad.ds.kcl.ac.uk> Message-ID: <4B956826.7010408@vneubert.com> Species is Human, tissue is lung. > What species, V? > More info? > Them TGF ligand /receptors are as difficult as FGF/FGFr, imho. > respect, > Carlos. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Mon Mar 8 15:27:11 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 8 15:27:15 2010 Subject: [Histonet] Embedding Forceps In-Reply-To: <184488.93528.qm@web65710.mail.ac4.yahoo.com> Message-ID: <493953.12699.qm@web65712.mail.ac4.yahoo.com> Having a pointed forceps is less of an issue than working with them delicately, and using just the pressure needed. Ren? J. --- On Mon, 3/8/10, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] Embedding Forceps To: "histonet" Date: Monday, March 8, 2010, 2:27 PM Anyone use special forceps for embedding?? I have some recent concerns that some of the forceps out there may be to pointy/sharp for delicate tissue (ie. skin).? Any insight?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alexandra.meinl <@t> gmail.com Mon Mar 8 15:35:30 2010 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Mon Mar 8 15:35:39 2010 Subject: [Histonet] Positive control tissues for RANKL and/or OPG needed Message-ID: Hello Histonetters, Does anyone have experience with nice positive control tissues for RANKL and/or OPG (except bone)? Both are soluble factors, but is there a chance to produce a reasonably discrete staining (of secretory vesicles?) in any organ? Alexandra ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.meinl@trauma.lbg.ac.at From STACEY.LANGENBERG <@t> UCDENVER.EDU Mon Mar 8 15:51:10 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Mon Mar 8 15:51:14 2010 Subject: [Histonet] Comparison of Leica Peloris to Microm STP 420 D Message-ID: <1F70FCBB6D4EC549B2ADF69B9F9EAC033EE06AEFBD@STEAMBOAT.ucdenver.pvt> Can anyone give me some pros and cons on these 2 rapid processors? Stacey Langenberg HT (ASCP) QIHC CU Dermatopathology Consultants 12635 E. Montveiw Blvd. Suite 160 Aurora, CO 80045 (720) 859-3559 From POWELL_SA <@t> mercer.edu Mon Mar 8 19:41:25 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Mar 8 19:41:32 2010 Subject: [Histonet] Reminder: Deadline for hotel rates for Georgia meeting Message-ID: <9BF995BC0E47744E9673A41486E24EE22429A7E244@MERCERMAIL.MercerU.local> Just a friendly reminder, the Evergreen Marriott Convention Resort is holding the discount rate for the Georgia Society for Histotechnology meeting (Stone Mountain, Ga, March 26-28th) until this Friday, March 12th. After then, the rates go back to their normal rates. Please make your reservations as soon as possible and definitely before Friday. There is a link to the hotel on the GSH website, www.histosearch.com/gsh, symposium page. You will find the link just below the 2010 GSH program and the registration form links. Click on the hotel link and it will take you directly to reservations and already has our meeting code entered. All you do is finish the required information. Late fee for registering for the meeting will apply after March 15th, so beat the deadlines. Make your plans now and attend a great meeting. See you at Stone Mountain. Also don't forget to celebrate National Histotechnology Professionals Day, March 10th, that is this Wednesday. Have a party, bake a cake, take the day off, whatever, but do advertise it to your employer. Shirley Powell GSH Secretary From greenjumpyone <@t> hotmail.com Mon Mar 8 19:55:08 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Mon Mar 8 19:55:13 2010 Subject: [Histonet] (no subject) Message-ID: http://barowh.republika.pl/y7ZiEqxaqc.html _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/ From greenjumpyone <@t> hotmail.com Mon Mar 8 19:56:30 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Mon Mar 8 19:56:34 2010 Subject: [Histonet] (no subject) Message-ID: http://www.bn.sqad.prv.pl/vJh0Rw4xdy.html _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/ From amosbrooks <@t> gmail.com Mon Mar 8 20:12:58 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Mar 8 20:13:05 2010 Subject: [Histonet] TGF beta on FFPE Message-ID: <582736991003081812r7a8b164cr44e1f89e67c2c6d7@mail.gmail.com> Hi, If anyone has a good answer for this, please share it. I have been asked to do this before and haven't had much confidence in the results. Thanks, Amos ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 8 Mar 2010 11:29:49 +0100 (CET) > From: histonet.nospam@vneubert.com > Subject: [Histonet] TGF beta on FFPE > To: histonet@lists.utsouthwestern.edu > Message-ID: <20100308102949.AFC24C299FED@dd15630.kasserver.com> > Content-Type: text/plain; charset="utf-8" > > Hello Histonet, > > I'd like to know if anyone of you has experience with TGF beta receptor and > TGF beta ligand IHC on FFPE tissue. > > Any input will be highly appreciated :) > > Thanks a lot > > V. Neubert > > From GauchV <@t> mail.amc.edu Tue Mar 9 07:21:45 2010 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Tue Mar 9 07:21:53 2010 Subject: [Histonet] Stainers/Coverslippers Message-ID: Just a quick thank you to all who responded to my inquiry about stainers/coverslippers...I am bringing all the info to the powers that be so we can start checking out some of these units. I appreciate all your help, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From b-frederick <@t> northwestern.edu Tue Mar 9 07:50:31 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Mar 9 07:50:38 2010 Subject: [Histonet] TGF beta on FFPE In-Reply-To: <582736991003081812r7a8b164cr44e1f89e67c2c6d7@mail.gmail.com> Message-ID: Which receptor? We run BR1,BR2and BR3 on FFPE as well as B1,B2 B3. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Monday, March 08, 2010 8:13 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TGF beta on FFPE Hi, If anyone has a good answer for this, please share it. I have been asked to do this before and haven't had much confidence in the results. Thanks, Amos ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 8 Mar 2010 11:29:49 +0100 (CET) > From: histonet.nospam@vneubert.com > Subject: [Histonet] TGF beta on FFPE > To: histonet@lists.utsouthwestern.edu > Message-ID: <20100308102949.AFC24C299FED@dd15630.kasserver.com> > Content-Type: text/plain; charset="utf-8" > > Hello Histonet, > > I'd like to know if anyone of you has experience with TGF beta receptor and > TGF beta ligand IHC on FFPE tissue. > > Any input will be highly appreciated :) > > Thanks a lot > > V. Neubert > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Tue Mar 9 08:51:51 2010 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Mar 9 08:51:58 2010 Subject: [Histonet] Surgical veterinary reference manual Message-ID: <4f016b691003090651p34c243a4le49252dba343fa98@mail.gmail.com> Hi I need to locate a reference manual for veterinary surgical pathology - if anyone knows of one would you please contact me? Thanks Vikki Baker From Lesley.Smith <@t> ramsayhealth.co.uk Tue Mar 9 08:51:46 2010 From: Lesley.Smith <@t> ramsayhealth.co.uk (Smith, Lesley) Date: Tue Mar 9 08:54:49 2010 Subject: [Histonet] Eosin too pink Message-ID: <37ACDF51E6FCB146B2D92527B733EB3599CCA5@uksbirs-ukmg01.ukramsay.rhc.local> Our Pathologists have, over the last few weeks, complained that the eosin is too pink. We have not altered our procedures or reagents in any way. It seems to be particularly bad in endoscopic biopsies. Thanks Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk ************************************************ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. From cbarone <@t> NEMOURS.ORG Tue Mar 9 08:56:18 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Tue Mar 9 08:56:22 2010 Subject: [Histonet] Region II Symposium Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7AAE@wlmmsx01.nemours.org> Histonetter's, Members of Region II and beyond.: Save the date for the 2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic City, N.J Come for a day. Come for the weekend. For more information about registration and accomodations contact Michele French: 609-818-3278 / michele.french@bms.com. Excellent Speakers, new workshops, vendor fair and of course...there is always the beach...(for studying, of course!). Stay tuned to the histonet, for more information regarding Seminars and Workshops. See you there. Carol Barone - Region II Director From dchihc <@t> yahoo.com Tue Mar 9 09:32:08 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Tue Mar 9 09:32:12 2010 Subject: [Histonet] Full Time Day Shift Position Message-ID: <954777.20407.qm@web43514.mail.sp1.yahoo.com> At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday.?Must be ASCP registered HT or HTL (or eligible...must become registered within 18 months of hire date). Lab Automation includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura?XPress 50 rapid tissue processor, Leica cryostats. Duties include embedding, microtomy, ihc and special stains, and frozen sections. DCH is a 500 plus bed regional medical center. We process around 12,000 to 13,000 specimens annually.? Interested candidates please contact Sherrie Faulkner?(recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From sweething63 <@t> msn.com Tue Mar 9 09:50:34 2010 From: sweething63 <@t> msn.com (R J VAZQUEZ) Date: Tue Mar 9 09:50:39 2010 Subject: [Histonet] Summer enployment Message-ID: Hello, I am attending school at this momment, but this coming summer I am looking for part/full time work only for the summer. I am on my way to getting an associates so I can take the histology test. I am a Mohs technician (OHSU) almost 9 years, but was laid off last June, I also do histology (cutting and special stains). I am also interested in Clinical Reseach studies. If you are in need of summer help or tech relief email me and I will be glad to send you a resume. Best Regards, Robyn Vazquez From mwfolsom <@t> rgbio.com Tue Mar 9 10:17:51 2010 From: mwfolsom <@t> rgbio.com (Michael Folsom) Date: Tue Mar 9 10:17:47 2010 Subject: [Histonet] Butyl alcohol In-Reply-To: <669973B45D49DA43B0FA9AC9609CBF54042376CF@MAPMAIL01.bsci.bossci.com> References: <669973B45D49DA43B0FA9AC9609CBF54042376CF@MAPMAIL01.bsci.bossci.com> Message-ID: <1268151471.7815.18.camel@chico.322tulane.org> Hi: I'm not sure if this helps but Botanists have long used tert-butanol (not N-butanol) to embed tissue in paraffin. This classic protocol was popularized by D. A. Johansen in the mid 1930's and involves transition from water to alcohol to t-butanol (I can supply more detailed info if you need it). Once in 100% t-butanol tissue is transitioned to light paraffin oil. The final stage involves filling some large shell vials 1/3 full with paraffin and letting them harden. Tissue plus paraffin oil is added to the vials (volume of paraffin oil + tissue should be be approximately equal to the amount of paraffin). Vials are now placed in an oven for paraffin to melt and infiltration to begin. Besides doing enough changes of paraffin to infiltrate the tissue and remove all the paraffin oil that's about it. From there its the old embed, section and enjoy! Hope this helps - Mike Folsom Rio Grande Biological mwfolsom@rgbio.com On Mon, 2010-03-08 at 09:00 -0600, Krueger, Todd wrote: > Does anyone have a procedure for processing with N-butyl Alcohol. We > need to find a procedure w/o xylene. > Thanks > > Todd Krueger > > HTL(ASCP)CM > > Boston Scientific > > 2 Scimed Place, P121 > > Osseo, MN 55311 > > Phone: 763-694-5709 > > Fax: 763-694-5505 > > e-mail: todd.krueger@bsci.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Mar 9 10:25:56 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Mar 9 10:26:00 2010 Subject: [Histonet] Surgical veterinary reference manual In-Reply-To: <4f016b691003090651p34c243a4le49252dba343fa98@mail.gmail.com> References: <4f016b691003090651p34c243a4le49252dba343fa98@mail.gmail.com> Message-ID: A Google search for "veterinary surgical pathology book" brings up lots! John Kiernan Anatomy, UWO London, Canada. = = = ----- Original Message ----- From: Victoria Baker Date: Tuesday, March 9, 2010 9:53 Subject: [Histonet] Surgical veterinary reference manual To: histonet > Hi > > I need to locate a reference manual for veterinary surgical > pathology - if > anyone knows of one would you please contact me? > > Thanks > > Vikki Baker > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cruffin <@t> valleycare.com Tue Mar 9 10:33:04 2010 From: cruffin <@t> valleycare.com (Carole Ruffin) Date: Tue Mar 9 10:33:15 2010 Subject: [Histonet] Please remove me from your list. Thank you Message-ID: <4B9607BF.1A81.00F2.0@valleycare.com> Please remove me from your list. Thank you This message and any included attachments are from ValleyCare Health System and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. From chak_bou <@t> yahoo.com Tue Mar 9 12:45:54 2010 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Tue Mar 9 12:45:58 2010 Subject: [Histonet] Helicobacter Pylori antibody Message-ID: <591938.93716.qm@web58105.mail.re3.yahoo.com> Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me?where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Mar 9 12:52:36 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Mar 9 12:53:42 2010 Subject: [Histonet] Helicobacter Pylori antibody In-Reply-To: <591938.93716.qm@web58105.mail.re3.yahoo.com> References: <591938.93716.qm@web58105.mail.re3.yahoo.com> Message-ID: I have ordered mine from Cell Marque. We use it at 1/50 dilution with a 30 minute incubation at RT on the Dako Autostainers. Works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain [chak_bou@yahoo.com] Sent: Tuesday, March 09, 2010 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Mar 9 12:58:14 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Mar 9 12:58:19 2010 Subject: [Histonet] Helicobacter Pylori antibody In-Reply-To: <591938.93716.qm@web58105.mail.re3.yahoo.com> Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF7E4@UWHC-MAIL01.uwhis.hosp.wisc.edu> Hi Chakib, We use Ventana's (manufactured by Cell Marque) H. Pylori on their instruments. The protocol is specific to the instruments but involves a routine HIER step and Avidin/Biotin blocking. The antibody itself is ready-to-use but is available from Cell Marque as a concentrate as well. The protocol produces a very clean stain; essential since these bugs are so small. Hope this helps, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain Sent: Tuesday, March 09, 2010 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me?where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From elciba <@t> hotmail.com Tue Mar 9 13:00:49 2010 From: elciba <@t> hotmail.com (ricky hachy) Date: Tue Mar 9 13:00:57 2010 Subject: [Histonet] Tissue Tek Message-ID: I need the 2 parafin pots for a Tissue Tek/Sakura tissue processor working or not . Anyone has them ? Regards Ricky _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/ From cpyse <@t> x-celllab.com Tue Mar 9 13:16:08 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Mar 9 13:16:39 2010 Subject: [Histonet] Helicobacter Pylori antibody In-Reply-To: <591938.93716.qm@web58105.mail.re3.yahoo.com> References: <591938.93716.qm@web58105.mail.re3.yahoo.com> Message-ID: <000001cabfbc$f93ecdc0$ebbc6940$@com> We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20 minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare) 10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes hematoxylin(Dako). Works great. Cindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain Sent: Tuesday, March 09, 2010 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me?where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Tue Mar 9 13:52:54 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Mar 9 13:52:58 2010 Subject: [Histonet] Helicobacter Pylori antibody In-Reply-To: <591938.93716.qm@web58105.mail.re3.yahoo.com> References: <591938.93716.qm@web58105.mail.re3.yahoo.com> Message-ID: <544545.25352.qm@web54204.mail.re2.yahoo.com> We use Cell Marque's Polyclonal H. Pylori? (CMC435) at a 1:100 dilution?on the Ventana BenchMark for 32 minutes, Protease 1 for 12 minutes. ? ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! ________________________________ From: Chakib Boussahmain To: histonet@lists.utsouthwestern.edu Sent: Tue, March 9, 2010 11:45:54 AM Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me?where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Tue Mar 9 14:00:43 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Mar 9 14:00:47 2010 Subject: [Histonet] Eosin too pink In-Reply-To: <37ACDF51E6FCB146B2D92527B733EB3599CCA5@uksbirs-ukmg01.ukramsay.rhc.local> References: <37ACDF51E6FCB146B2D92527B733EB3599CCA5@uksbirs-ukmg01.ukramsay.rhc.local> Message-ID: <382091.74830.qm@web54203.mail.re2.yahoo.com> We add 100mL of 100% alcohol to 150mL of Eosin to tone it down each day.? ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! ________________________________ From: "Smith, Lesley" To: histonet@lists.utsouthwestern.edu Sent: Tue, March 9, 2010 7:51:46 AM Subject: [Histonet] Eosin too pink Our Pathologists have, over the last few weeks, complained that the eosin is too pink. We have not altered our procedures or reagents in any way. It seems to be particularly bad in endoscopic biopsies. Thanks Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk ************************************************ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Tue Mar 9 14:08:17 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Mar 9 14:08:18 2010 Subject: [Histonet] hydrometer Message-ID: <24A4826E8EF0964D86BC5317306F58A54257BC3090@mmc-mail.ad.mhsil.com> Anybody have any idea where to purchase a simple hydrometer for checking the water content in alcohols? I found one at TBS but do not know if it will fit our purposes since it says use with a specific recycler. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From DKBoyd <@t> chs.net Tue Mar 9 14:12:05 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Mar 9 14:12:08 2010 Subject: [Histonet] hydrometer In-Reply-To: <24A4826E8EF0964D86BC5317306F58A54257BC3090@mmc-mail.ad.mhsil.com> Message-ID: We ordered ours from Fisher Scientific. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From cbarone <@t> NEMOURS.ORG Tue Mar 9 16:02:30 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Tue Mar 9 16:02:35 2010 Subject: [Histonet] Region II mtg addiiton and up-date: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7AC2@wlmmsx01.nemours.org> Histonetter's, Region II up-date: Save the date for the 2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic City, N.J ...Come for a day... or come for the weekend. Excellent Speakers, new workshops, vendor fair and of course...there is always the beach. ..(for studying, of course!). Stay tuned to the histonet, for more information regarding specific seminars and workshops. More to come... Information on registration will be posted on the histonet in April. So keep up with the histonet...for lots of great networking.... and info on the Region II Symposium See you there. Carol Barone - Region II Director...Oh!...and keep networking! It is the best educational tool of all! From DKnutson <@t> primecare.org Tue Mar 9 16:27:30 2010 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Tue Mar 9 16:27:37 2010 Subject: [Histonet] p16 Message-ID: <1E0E2B14C709174B8AC2BE0AE7F768338FE967C68A@EXCHANGE2K7.staprimecare.org> Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From rjbuesa <@t> yahoo.com Tue Mar 9 16:43:54 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 9 16:43:57 2010 Subject: [Histonet] Butyl alcohol In-Reply-To: <1268151471.7815.18.camel@chico.322tulane.org> Message-ID: <169396.76443.qm@web65709.mail.ac4.yahoo.com> As an additional note: paraffin oil = mineral oil. Ren? J. --- On Tue, 3/9/10, Michael Folsom wrote: From: Michael Folsom Subject: Re: [Histonet] Butyl alcohol To: "Krueger, Todd" Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, March 9, 2010, 11:17 AM Hi: I'm not sure if this helps but Botanists have long used tert-butanol (not N-butanol) to embed tissue in paraffin.? This classic? protocol was popularized by D. A. Johansen in the mid 1930's and involves transition from water to alcohol to t-butanol (I can supply more detailed info if you need it).? Once in 100% t-butanol tissue is transitioned to light paraffin oil.? The final stage involves filling some large shell vials 1/3 full with paraffin and letting them harden.? Tissue plus paraffin oil is added to the vials (volume of paraffin oil + tissue should be be approximately equal to the amount of paraffin).? Vials are now placed in an oven for paraffin to melt and infiltration to begin.? Besides doing enough changes of paraffin to infiltrate the tissue and remove all the paraffin oil that's about it.? From there its the old embed, section and enjoy! Hope this helps - Mike Folsom Rio Grande Biological mwfolsom@rgbio.com On Mon, 2010-03-08 at 09:00 -0600, Krueger, Todd wrote: > Does anyone have a procedure for processing with N-butyl Alcohol. We > need to find a procedure w/o xylene. > Thanks >? > Todd Krueger > > HTL(ASCP)CM > > Boston Scientific > > 2 Scimed Place, P121 > > Osseo, MN 55311 > > Phone: 763-694-5709 > > Fax: 763-694-5505 > > e-mail: todd.krueger@bsci.com > >? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cscampbe <@t> uci.edu Tue Mar 9 19:06:21 2010 From: cscampbe <@t> uci.edu (cscampbe@uci.edu) Date: Tue Mar 9 19:06:24 2010 Subject: [Histonet] Masson Trichrome, Alligator Heart Tissue, colors turning out purple Message-ID: <88928dfe83ca47dbf12638e73258f4a1.squirrel@webmail.uci.edu> To Histonet: This is my first time mailing the histonet community. Coincidentally, this is my first venture into histology. My lab is conducting research on alligator heart tissue. At the moment, I am attempting to stain my slides using Masson Trichrome to differentiate muscle from connective tissue. Results have not been optimal. The slides turn out to be more purple than I had anticipated (comparing to pictures from journals). The red can be told apart from the teal, but the colors are not bright. Here is the procedure I am using: 1. Deparaffinize and hydrate to water 2. Iron Alum, 30 min 3. Wash in Dis. Water, 5 min 4. Hematoxylin stain, 30 min 5. Wash in Dis. Water, 5 min 6. Differentiate in saturated aq. picric acid, 1 min 7. Wash in dis. water, 10 min 8. Stain in acid fuschin, 5 min 9. Rinse in water 10. Stain in Ponceau de Xylidene, 5 min 11. Rinse in water 12. Differentiate in Phosphomolybdic acid, 5 min 13. Fast Green, 2 min 14. Differentiate in 1% aq. acetic acid, 5 min 15. Dehydrate 16. xylene (two washes, 3 min each) I would greatly appreciate any advice on how to modify my procedure to better stain the cardiac tissue. Or should I be using a different trichrome method better suited to this particular tissue? Thank you! -Colin From jinhui <@t> uta.edu Wed Mar 10 00:44:08 2010 From: jinhui <@t> uta.edu (Shen, Jinhui) Date: Wed Mar 10 00:44:21 2010 Subject: [Histonet] Need help with FISH staining protocol on frozen tissue sections Message-ID: Hi everyone, Has anyone done FISH staining on mice tissue frozen sections for Y chromosome? I need help with a successful protocol. Recently I started to do FISH staining on mice liver frozen sections for chromosome Y, but got no positive results even on male samples yet. The probe I used was IDMF1057 Green label Chr Y probe from ID Labs Inc. (www.idlabs.com). They have a protocol online probably for cultured cells (http://www.idlabs.com/product/datasheets/IDMF1057.pdf?PHPSESSID=b39f16507cf76b69495166c651f0b52c) which I haven't tried yet. I followed the following protocol for frozen sections: 1. Fix sections in 4% PFA at 4C for 10 mins, then wash in PBS. 2. Treat the sections with 0.01% Pepsin, 37C 10min, wash in PBS. 3. Dehydrate the slides using 70% Ethanol (2minsX2), 90% Ethanol (2mins X2), 100% Ethanol (5 mins X1).Then Age the slides at 65C for one hour. 4. Mix the probe and hybridization buffer. Denature the probe by incubate it at 65C for 10 mins, then hold it at 37C for 30 - 60 mins. 5. Denature the sections by incubate them in denaturing solution (formamide:2XSSC, 70:30) at 75C for 2 mins. 6. Quench the slides in 4C 70% Ethanol for 4 mins. Dehydrate the slides as step 3. 7. Apply the probe and hybridize at 37C overnight. 8. Immerse the slides in 1XSSC for 5 mins to remove the cover slips. 9. Wash slides in stringency solution (formamide:2XSSC, 50:50) at 45C, 5 mins X2. 10. Wash slides in 1XSSC at 45C, 5minsX2. 11. Wash slides in detergent wash solution at 45C, 5minX1. 12. Rinse slides in PBS. 13. Air dry for a couple of minutes and counterstain with DAPI. 14. Mount the slides and observe the results or keep in 4C for late check. Are there any problems with this procedure? Please help me to check, or if you could recommend a working protocol you had used, I would really appreciate. Thanks, Jinhui From settembr <@t> umdnj.edu Wed Mar 10 07:06:57 2010 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Mar 10 07:07:13 2010 Subject: [Histonet] p16 Message-ID: Hi Deanne, My understanding is that you must get p16 from MTM Laboratories. They have some sort of monopoly right now. I still have some leftover from Cell Marque and when I run low or the antibody is about to expire I must also go to MTM. I currently use the Cell Marque p16 Ready To Use by pretreating with Dako's TRS for 40min in steamer, and detect with labelled polymer. Dako's Envision + Mouse kit. It works nicely. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Knutson, Deanne" 03/09/10 5:27 PM >>> Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Wed Mar 10 07:25:25 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Mar 10 07:26:01 2010 Subject: [Histonet] p16 In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F768338FE967C68A@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F768338FE967C68A@EXCHANGE2K7.staprimecare.org> Message-ID: <000001cac055$24c65010$6e52f030$@com> We purchase our p16 through MTM laboratories reference # 9517. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Tuesday, March 09, 2010 5:28 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] p16 Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kelleydurden <@t> pathology.ufl.edu Wed Mar 10 08:15:19 2010 From: kelleydurden <@t> pathology.ufl.edu (Durden, Kelley) Date: Wed Mar 10 08:15:59 2010 Subject: [Histonet] Masson Trichrome, Alligator Heart Tissue, colors turning out purple Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE591DBE3A@HSC-CMS01.ad.ufl.edu> I've been using the Masson Trichrome kit from Richard Allan since 2001 and I've always gotten really great results. It is a little more expensive than using home made reagents but the results are very consistent. Kelley From greenjumpyone <@t> hotmail.com Wed Mar 10 08:44:18 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Wed Mar 10 08:44:21 2010 Subject: [Histonet] Microwave Tissue Processor Protocols In-Reply-To: References: Message-ID: I am working on the validation of our microwave processors and have noticed that the tissues are a bit dry. Would anyone be willing to share their protocol timings for their fixation, ethanol, isopropyl and wax impregnation steps? We are using the Histos-5 microwaves from Milestone and are utilizing the 2mm (for the smalls) and the 3mm (for the larger specimens) default program settings. thanks for any help! Michelle ps, my apologies to the board for someone spamming the board using a spoof of my email address. :o( _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/ From AJohnson <@t> aipathology.com Wed Mar 10 08:54:11 2010 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Wed Mar 10 08:54:17 2010 Subject: [Histonet] HistoDay Message-ID: <704247D5A09D004C9E6B115138D1703A1C4B89@hpserv001.aipathology.local> Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! From sbreeden <@t> nmda.nmsu.edu Wed Mar 10 09:02:17 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Mar 10 09:02:21 2010 Subject: [Histonet] Histology Professionals Day Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46F26@nmdamailsvr.nmda.ad.nmsu.edu> If it weren't for a med-tech-turned-pathologist that took me up on my interest in becoming trained in histology (1967), I would not have just marked my 41st year as a histologist. Back then, you just had to have OJT and study independently for your written and practical tests. I had no idea what I was getting into but it was one of the absolute best choices I ever made. To pretend to quote some baseball player, "Histology been berry, berry good to me"! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From NSEARCY <@t> swmail.sw.org Wed Mar 10 09:07:17 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Wed Mar 10 09:07:27 2010 Subject: [Histonet] Histology Professionals Day In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F26@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46F26@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4B976145.5D38.00EF.0@swmail.sw.org> I too "stumbled" into the profession in 1967 and share Sara's sentiments. I sent the following to our senior staff as well as the supervisory group of our laboratory: Today is the first annual National Histotechnology day and is being celebrated by the histology staff by a morning breakfast ( in order that the entire staff can participate) and the wearing of special t-shirts. In spite of almost total anonymity to most of the general public, as well as a considerable amount of health care professionals, these highly skilled individuals play a vital role in healthcare. Wish them congratulations. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Breeden, Sara" 3/10/2010 9:02 AM >>> If it weren't for a med-tech-turned-pathologist that took me up on my interest in becoming trained in histology (1967), I would not have just marked my 41st year as a histologist. Back then, you just had to have OJT and study independently for your written and practical tests. I had no idea what I was getting into but it was one of the absolute best choices I ever made. To pretend to quote some baseball player, "Histology been berry, berry good to me"! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From alyssa <@t> alliedsearchpartners.com Wed Mar 10 09:09:18 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Mar 10 09:09:24 2010 Subject: [Histonet] Happy HPD to our "Unsung Heroes" Message-ID: Allied Search Partners wants to wish you a Happy Histology Professionals Day! Histology professionals are the unsung heroes behind the laboratory doors. The patient may not know you but what you all do is the absolute best for them because what you do affects their lives and their loved ones lives. I want to thank each and every one of you for doing what you do behind closed doors away from the surgeons who see the patients and actually get to remove the problem. Thank you for being the Unspoken Heroes!! Please contact us today to inquire about Histology jobs in your area. Alyssa Peterson Director of Recruitment Allied Search Partners Alyssa@alliedsearchpartners.com WWW.ALLIEDSEARCHPARTNERS.COM From leiker <@t> buffalo.edu Wed Mar 10 09:13:35 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Mar 10 09:13:42 2010 Subject: [Histonet] Need help with FISH staining protocol on frozen tissue sections In-Reply-To: References: Message-ID: I have been using IDLabs porcine Y chromosome green label probe on frozen pig tissue with good results most of the time. You say you denature the probe and then hold it at 37oC...I would think that would allow the probe to reanneal to itself since you also hybridize later on at that temp...try following the IDLabs protocol where they say to co-denature the probe and the slide. I do this for my frozen sections and it works. Regards, Merced --On Wednesday, March 10, 2010 12:44 AM -0600 "Shen, Jinhui" wrote: > Hi everyone, > > Has anyone done FISH staining on mice tissue frozen sections for Y > chromosome? I need help with a successful protocol. > > Recently I started to do FISH staining on mice liver frozen sections for > chromosome Y, but got no positive results even on male samples yet. The > probe I used was IDMF1057 Green label Chr Y probe from ID Labs Inc. > (www.idlabs.com). They have a protocol online probably for cultured cells > (http://www.idlabs.com/product/datasheets/IDMF1057.pdf?PHPSESSID=b39f1650 > 7cf76b69495166c651f0b52c) which I haven't tried yet. I followed the > following protocol for frozen sections: > > 1. Fix sections in 4% PFA at 4C for 10 mins, then wash in PBS. > > 2. Treat the sections with 0.01% Pepsin, 37C 10min, wash in PBS. > > 3. Dehydrate the slides using 70% Ethanol (2minsX2), 90% Ethanol (2mins > X2), 100% Ethanol (5 mins X1).Then Age the slides at 65C for one hour. > > 4. Mix the probe and hybridization buffer. Denature the probe by incubate > it at 65C for 10 mins, then hold it at 37C for 30 - 60 mins. > > 5. Denature the sections by incubate them in denaturing solution > (formamide:2XSSC, 70:30) at 75C for 2 mins. > > 6. Quench the slides in 4C 70% Ethanol for 4 mins. Dehydrate the slides > as step 3. > > 7. Apply the probe and hybridize at 37C overnight. > > 8. Immerse the slides in 1XSSC for 5 mins to remove the cover slips. > > 9. Wash slides in stringency solution (formamide:2XSSC, 50:50) at 45C, 5 > mins X2. > > 10. Wash slides in 1XSSC at 45C, 5minsX2. > > 11. Wash slides in detergent wash solution at 45C, 5minX1. > > 12. Rinse slides in PBS. > > 13. Air dry for a couple of minutes and counterstain with DAPI. > > 14. Mount the slides and observe the results or keep in 4C for late check. > > Are there any problems with this procedure? Please help me to check, or > if you could recommend a working protocol you had used, I would really > appreciate. > > Thanks, > > Jinhui > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From SLB <@t> stowers.org Wed Mar 10 09:13:46 2010 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Wed Mar 10 09:13:53 2010 Subject: [Histonet] RE: Histology Professionals Day In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F26@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46F26@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Sally, I have pretty much the same story. Back in 1970 I applied for a pathology secretary position and the pathologist asked me if I could sew, which meant he wondered if I could do intricate work with my hands. In my spare time, I trained in histology and took my registry in 1973. It was the best decision I ever made to take him up on his offer. I love, love, love being a histologist and hope that my health remains good so I can do it for many more years to come. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, March 10, 2010 9:02 AM To: histonet Subject: [Histonet] Histology Professionals Day If it weren't for a med-tech-turned-pathologist that took me up on my interest in becoming trained in histology (1967), I would not have just marked my 41st year as a histologist. Back then, you just had to have OJT and study independently for your written and practical tests. I had no idea what I was getting into but it was one of the absolute best choices I ever made. To pretend to quote some baseball player, "Histology been berry, berry good to me"! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMP <@t> stowers.org Wed Mar 10 09:13:45 2010 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Wed Mar 10 09:13:56 2010 Subject: [Histonet] RE: HistoDay In-Reply-To: <704247D5A09D004C9E6B115138D1703A1C4B89@hpserv001.aipathology.local> References: <704247D5A09D004C9E6B115138D1703A1C4B89@hpserv001.aipathology.local> Message-ID: Here's a quote that we have posted on our Histology event board "Ah, the Professional Hostologist! Walk in awe when you meet one....because there are only a few people who can produce something as beautiful and useful as a slide." by Drs. William Osler and F.E. Mohs----just about says it all, doesn't it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, March 10, 2010 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoDay Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMP <@t> stowers.org Wed Mar 10 09:18:54 2010 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Wed Mar 10 09:19:04 2010 Subject: [Histonet] RE: HistoDay In-Reply-To: <704247D5A09D004C9E6B115138D1703A1C4B89@hpserv001.aipathology.local> References: <704247D5A09D004C9E6B115138D1703A1C4B89@hpserv001.aipathology.local> Message-ID: Okay--so I don't proof read very well--that was supposed to be 'histologist'--not hostologist in my last post--although there are days when hostility abounds in clinical pathology!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, March 10, 2010 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoDay Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed Mar 10 09:21:42 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Mar 10 09:21:46 2010 Subject: [Histonet] Histo Professionals Day Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46F28@nmdamailsvr.nmda.ad.nmsu.edu> Perhaps what each of us ought to do is write a quick line naming the one person that got you involved in histology and how they did that. I'd be willing to gather them and pass them on to NSH so they could post it at the Seattle meeting. If you'd like to do this, send the email to nmhisto@comcast.net and I'll collect. Just an idea... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From dholmes <@t> anatomy.umsmed.edu Wed Mar 10 09:42:27 2010 From: dholmes <@t> anatomy.umsmed.edu (Dianne Holmes) Date: Wed Mar 10 09:42:50 2010 Subject: Fwd: [Histonet] RE: HistoDay References: <704247D5A09D004C9E6B115138D1703A1C4B89@hpserv001.aipathology.local> Message-ID: That's OK cause I re-typed and posted it on my cutting wall along with pics of my favorite slides produced in this lab. Being proud of your work is the best 'teaching tool' I have found. >>> "Marsh, Nannette" 3/10/2010 9:18 AM >>> Okay--so I don't proof read very well--that was supposed to be 'histologist'--not hostologist in my last post--although there are days when hostility abounds in clinical pathology!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, March 10, 2010 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoDay Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From Janet.Bonner <@t> FLHOSP.ORG Wed Mar 10 09:52:33 2010 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed Mar 10 09:53:28 2010 Subject: [Histonet] RE: HistoDay References: <704247D5A09D004C9E6B115138D1703A1C4B89@hpserv001.aipathology.local> Message-ID: <5F31F38C96781A4FBE3196EBC22D4780015FB20F@fhosxchmb006.ADVENTISTCORP.NET> At least it wasn't Hystologist!! @:) Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marsh, Nannette Sent: Wed 3/10/2010 10:18 AM To: 'Amy Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: HistoDay Okay--so I don't proof read very well--that was supposed to be 'histologist'--not hostologist in my last post--although there are days when hostility abounds in clinical pathology!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, March 10, 2010 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoDay Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From lblazek <@t> digestivespecialists.com Wed Mar 10 09:54:12 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Mar 10 09:56:25 2010 Subject: [Histonet] Histo Day Message-ID: <5A2BD13465E061429D6455C8D6B40E390E998D5455@IBMB7Exchange.digestivespecialists.com> Somewhere back in the late 60's and somewhere in Michigan there was a cytotech named Beryl and a histotech named "Charlie Brown" that introduced me to the world of the lab while I was in high school. I fell in love with histology then and have been ever since then! So THANK YOU Beryl and "Charlie Brown" from the bottom of my heart! Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com From POWELL_SA <@t> mercer.edu Wed Mar 10 10:06:48 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Mar 10 10:06:55 2010 Subject: [Histonet] RE: HistoDay In-Reply-To: <5F31F38C96781A4FBE3196EBC22D4780015FB20F@fhosxchmb006.ADVENTISTCORP.NET> References: <704247D5A09D004C9E6B115138D1703A1C4B89@hpserv001.aipathology.local> <5F31F38C96781A4FBE3196EBC22D4780015FB20F@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <9BF995BC0E47744E9673A41486E24EE2242A56C4F7@MERCERMAIL.MercerU.local> I can beat that one. My secretary ordered me flowers for today and explicitly spelled the message for the florist. The card got to me and I am a histoteshnologist. No one knows us. :) Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Wednesday, March 10, 2010 10:53 AM To: Marsh, Nannette; Amy Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: HistoDay At least it wasn't Hystologist!! @:) Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marsh, Nannette Sent: Wed 3/10/2010 10:18 AM To: 'Amy Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: HistoDay Okay--so I don't proof read very well--that was supposed to be 'histologist'--not hostologist in my last post--although there are days when hostility abounds in clinical pathology!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, March 10, 2010 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoDay Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Wed Mar 10 10:16:23 2010 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Mar 10 10:16:26 2010 Subject: [Histonet] Histo Day In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390E998D5455@IBMB7Exchange.digestivespecialists.com> Message-ID: I was working at a grocery store deli with a BS in biology when a teacher from the local technical college started there for something to do over the summer. He told me about a program called "Histotechnology" that boasted 100% placement after graduation. That was enough for me & I was interning in Milwaukee, WI two years later. I don't think anyone ever sets out to be a histotech, we all just sort of "fall into it". Happy HP Day All, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From baustin <@t> cbgbiotech.com Wed Mar 10 10:17:06 2010 From: baustin <@t> cbgbiotech.com (Beth Austin) Date: Wed Mar 10 10:17:15 2010 Subject: [Histonet] hydrometer Message-ID: CBG Biotech offers hydrometers. Regards, Beth Austin CBG Biotech 1-800-941-9484 ext 200 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Helicobacter Pylori antibody (Chakib Boussahmain) 2. RE: Helicobacter Pylori antibody (McMahon, Loralee A) 3. RE: Helicobacter Pylori antibody (Sebree Linda A) 4. Tissue Tek (ricky hachy) 5. RE: Helicobacter Pylori antibody (Cynthia Pyse) 6. Re: Helicobacter Pylori antibody (Kim Tournear) 7. Re: Eosin too pink (Kim Tournear) 8. hydrometer (Vickroy, Jim) 9. Re: hydrometer (DKBoyd@chs.net) 10. Region II mtg addiiton and up-date: (Barone, Carol ) 11. p16 (Knutson, Deanne) 12. Re: Butyl alcohol (Rene J Buesa) 13. Masson Trichrome, Alligator Heart Tissue, colors turning out purple (cscampbe@uci.edu) 14. Need help with FISH staining protocol on frozen tissue sections (Shen, Jinhui) 15. Re: p16 (Dana Settembre) 16. RE: p16 (Cynthia Pyse) 17. Masson Trichrome, Alligator Heart Tissue, colors turning out purple (Durden, Kelley) 18. Microwave Tissue Processor Protocols (Green JumpyOne) 19. HistoDay (Amy Johnson) 20. Histology Professionals Day (Breeden, Sara) 21. Re: Histology Professionals Day (Nita Searcy) 22. Happy HPD to our "Unsung Heroes" (Alyssa Peterson) 23. Re: Need help with FISH staining protocol on frozen tissue sections (Merced M Leiker) 24. RE: Histology Professionals Day (Beckham, Sharon) 25. RE: HistoDay (Marsh, Nannette) 26. RE: HistoDay (Marsh, Nannette) 27. Histo Professionals Day (Breeden, Sara) ---------------------------------------------------------------------- Message: 1 Date: Tue, 9 Mar 2010 10:45:54 -0800 (PST) From: Chakib Boussahmain Subject: [Histonet] Helicobacter Pylori antibody To: histonet@lists.utsouthwestern.edu Message-ID: <591938.93716.qm@web58105.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM ------------------------------ Message: 2 Date: Tue, 9 Mar 2010 13:52:36 -0500 From: "McMahon, Loralee A" Subject: RE: [Histonet] Helicobacter Pylori antibody To: Chakib Boussahmain , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I have ordered mine from Cell Marque. We use it at 1/50 dilution with a 30 minute incubation at RT on the Dako Autostainers. Works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain [chak_bou@yahoo.com] Sent: Tuesday, March 09, 2010 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 9 Mar 2010 12:58:14 -0600 From: "Sebree Linda A" Subject: RE: [Histonet] Helicobacter Pylori antibody To: "Chakib Boussahmain" , Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF7E4@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" Hi Chakib, We use Ventana's (manufactured by Cell Marque) H. Pylori on their instruments. The protocol is specific to the instruments but involves a routine HIER step and Avidin/Biotin blocking. The antibody itself is ready-to-use but is available from Cell Marque as a concentrate as well. The protocol produces a very clean stain; essential since these bugs are so small. Hope this helps, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain Sent: Tuesday, March 09, 2010 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 9 Mar 2010 19:00:49 +0000 From: ricky hachy Subject: [Histonet] Tissue Tek To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I need the 2 parafin pots for a Tissue Tek/Sakura tissue processor working or not . Anyone has them ? Regards Ricky _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/ ------------------------------ Message: 5 Date: Tue, 9 Mar 2010 14:16:08 -0500 From: "Cynthia Pyse" Subject: RE: [Histonet] Helicobacter Pylori antibody To: "'Chakib Boussahmain'" , Message-ID: <000001cabfbc$f93ecdc0$ebbc6940$@com> Content-Type: text/plain; charset="iso-8859-1" We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20 minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare) 10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes hematoxylin(Dako). Works great. Cindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain Sent: Tuesday, March 09, 2010 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 9 Mar 2010 11:52:54 -0800 (PST) From: Kim Tournear Subject: Re: [Histonet] Helicobacter Pylori antibody To: Chakib Boussahmain , Histonet Message-ID: <544545.25352.qm@web54204.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We use Cell Marque's Polyclonal H. Pylori (CMC435) at a 1:100 dilution on the Ventana BenchMark for 32 minutes, Protease 1 for 12 minutes. ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! ________________________________ From: Chakib Boussahmain To: histonet@lists.utsouthwestern.edu Sent: Tue, March 9, 2010 11:45:54 AM Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 9 Mar 2010 12:00:43 -0800 (PST) From: Kim Tournear Subject: Re: [Histonet] Eosin too pink To: "Smith, Lesley" , Histonet Message-ID: <382091.74830.qm@web54203.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We add 100mL of 100% alcohol to 150mL of Eosin to tone it down each day. ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! ________________________________ From: "Smith, Lesley" To: histonet@lists.utsouthwestern.edu Sent: Tue, March 9, 2010 7:51:46 AM Subject: [Histonet] Eosin too pink Our Pathologists have, over the last few weeks, complained that the eosin is too pink. We have not altered our procedures or reagents in any way. It seems to be particularly bad in endoscopic biopsies. Thanks Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk ************************************************ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 9 Mar 2010 14:08:17 -0600 From: "Vickroy, Jim" Subject: [Histonet] hydrometer To: "Histonet@lists.utsouthwestern.edu" Message-ID: <24A4826E8EF0964D86BC5317306F58A54257BC3090@mmc-mail.ad.mhsil.com> Content-Type: text/plain; charset="us-ascii" Anybody have any idea where to purchase a simple hydrometer for checking the water content in alcohols? I found one at TBS but do not know if it will fit our purposes since it says use with a specific recycler. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 9 Date: Tue, 9 Mar 2010 15:12:05 -0500 From: DKBoyd@chs.net Subject: Re: [Histonet] hydrometer To: "Vickroy, Jim" Cc: "Histonet@lists.utsouthwestern.edu" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We ordered ours from Fisher Scientific. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 10 Date: Tue, 9 Mar 2010 17:02:30 -0500 From: "Barone, Carol " Subject: [Histonet] Region II mtg addiiton and up-date: To: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7AC2@wlmmsx01.nemours.org> Content-Type: text/plain; charset="us-ascii" Histonetter's, Region II up-date: Save the date for the 2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic City, N.J ...Come for a day... or come for the weekend. Excellent Speakers, new workshops, vendor fair and of course...there is always the beach. ..(for studying, of course!). Stay tuned to the histonet, for more information regarding specific seminars and workshops. More to come... Information on registration will be posted on the histonet in April. So keep up with the histonet...for lots of great networking.... and info on the Region II Symposium See you there. Carol Barone - Region II Director...Oh!...and keep networking! It is the best educational tool of all! ------------------------------ Message: 11 Date: Tue, 9 Mar 2010 16:27:30 -0600 From: "Knutson, Deanne" Subject: [Histonet] p16 To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <1E0E2B14C709174B8AC2BE0AE7F768338FE967C68A@EXCHANGE2K7.staprimecare.org> Content-Type: text/plain; charset="us-ascii" Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ------------------------------ Message: 12 Date: Tue, 9 Mar 2010 14:43:54 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Butyl alcohol To: ToddKrueger , Michael Folsom Cc: histonet@lists.utsouthwestern.edu Message-ID: <169396.76443.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 As an additional note: paraffin oil = mineral oil. Reni J. --- On Tue, 3/9/10, Michael Folsom wrote: From: Michael Folsom Subject: Re: [Histonet] Butyl alcohol To: "Krueger, Todd" Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, March 9, 2010, 11:17 AM Hi: I'm not sure if this helps but Botanists have long used tert-butanol (not N-butanol) to embed tissue in paraffin. This classic protocol was popularized by D. A. Johansen in the mid 1930's and involves transition from water to alcohol to t-butanol (I can supply more detailed info if you need it). Once in 100% t-butanol tissue is transitioned to light paraffin oil. The final stage involves filling some large shell vials 1/3 full with paraffin and letting them harden. Tissue plus paraffin oil is added to the vials (volume of paraffin oil + tissue should be be approximately equal to the amount of paraffin). Vials are now placed in an oven for paraffin to melt and infiltration to begin. Besides doing enough changes of paraffin to infiltrate the tissue and remove all the paraffin oil that's about it. From there its the old embed, section and enjoy! Hope this helps - Mike Folsom Rio Grande Biological mwfolsom@rgbio.com On Mon, 2010-03-08 at 09:00 -0600, Krueger, Todd wrote: > Does anyone have a procedure for processing with N-butyl Alcohol. We > need to find a procedure w/o xylene. > Thanks > > Todd Krueger > > HTL(ASCP)CM > > Boston Scientific > > 2 Scimed Place, P121 > > Osseo, MN 55311 > > Phone: 763-694-5709 > > Fax: 763-694-5505 > > e-mail: todd.krueger@bsci.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 9 Mar 2010 17:06:21 -0800 From: cscampbe@uci.edu Subject: [Histonet] Masson Trichrome, Alligator Heart Tissue, colors turning out purple To: histonet@lists.utsouthwestern.edu Message-ID: <88928dfe83ca47dbf12638e73258f4a1.squirrel@webmail.uci.edu> Content-Type: text/plain;charset=iso-8859-1 To Histonet: This is my first time mailing the histonet community. Coincidentally, this is my first venture into histology. My lab is conducting research on alligator heart tissue. At the moment, I am attempting to stain my slides using Masson Trichrome to differentiate muscle from connective tissue. Results have not been optimal. The slides turn out to be more purple than I had anticipated (comparing to pictures from journals). The red can be told apart from the teal, but the colors are not bright. Here is the procedure I am using: 1. Deparaffinize and hydrate to water 2. Iron Alum, 30 min 3. Wash in Dis. Water, 5 min 4. Hematoxylin stain, 30 min 5. Wash in Dis. Water, 5 min 6. Differentiate in saturated aq. picric acid, 1 min 7. Wash in dis. water, 10 min 8. Stain in acid fuschin, 5 min 9. Rinse in water 10. Stain in Ponceau de Xylidene, 5 min 11. Rinse in water 12. Differentiate in Phosphomolybdic acid, 5 min 13. Fast Green, 2 min 14. Differentiate in 1% aq. acetic acid, 5 min 15. Dehydrate 16. xylene (two washes, 3 min each) I would greatly appreciate any advice on how to modify my procedure to better stain the cardiac tissue. Or should I be using a different trichrome method better suited to this particular tissue? Thank you! -Colin ------------------------------ Message: 14 Date: Wed, 10 Mar 2010 00:44:08 -0600 From: "Shen, Jinhui" Subject: [Histonet] Need help with FISH staining protocol on frozen tissue sections To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone, Has anyone done FISH staining on mice tissue frozen sections for Y chromosome? I need help with a successful protocol. Recently I started to do FISH staining on mice liver frozen sections for chromosome Y, but got no positive results even on male samples yet. The probe I used was IDMF1057 Green label Chr Y probe from ID Labs Inc. (www.idlabs.com). They have a protocol online probably for cultured cells (http://www.idlabs.com/product/datasheets/IDMF1057.pdf?PHPSESSID=b39f16507cf 76b69495166c651f0b52c) which I haven't tried yet. I followed the following protocol for frozen sections: 1. Fix sections in 4% PFA at 4C for 10 mins, then wash in PBS. 2. Treat the sections with 0.01% Pepsin, 37C 10min, wash in PBS. 3. Dehydrate the slides using 70% Ethanol (2minsX2), 90% Ethanol (2mins X2), 100% Ethanol (5 mins X1).Then Age the slides at 65C for one hour. 4. Mix the probe and hybridization buffer. Denature the probe by incubate it at 65C for 10 mins, then hold it at 37C for 30 - 60 mins. 5. Denature the sections by incubate them in denaturing solution (formamide:2XSSC, 70:30) at 75C for 2 mins. 6. Quench the slides in 4C 70% Ethanol for 4 mins. Dehydrate the slides as step 3. 7. Apply the probe and hybridize at 37C overnight. 8. Immerse the slides in 1XSSC for 5 mins to remove the cover slips. 9. Wash slides in stringency solution (formamide:2XSSC, 50:50) at 45C, 5 mins X2. 10. Wash slides in 1XSSC at 45C, 5minsX2. 11. Wash slides in detergent wash solution at 45C, 5minX1. 12. Rinse slides in PBS. 13. Air dry for a couple of minutes and counterstain with DAPI. 14. Mount the slides and observe the results or keep in 4C for late check. Are there any problems with this procedure? Please help me to check, or if you could recommend a working protocol you had used, I would really appreciate. Thanks, Jinhui ------------------------------ Message: 15 Date: Wed, 10 Mar 2010 08:06:57 -0500 From: "Dana Settembre" Subject: Re: [Histonet] p16 To: "'histonet@lists.utsouthwestern.edu'" , "Deanne Knutson" Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Deanne, My understanding is that you must get p16 from MTM Laboratories. They have some sort of monopoly right now. I still have some leftover from Cell Marque and when I run low or the antibody is about to expire I must also go to MTM. I currently use the Cell Marque p16 Ready To Use by pretreating with Dako's TRS for 40min in steamer, and detect with labelled polymer. Dako's Envision + Mouse kit. It works nicely. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Knutson, Deanne" 03/09/10 5:27 PM >>> Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 10 Mar 2010 08:25:25 -0500 From: "Cynthia Pyse" Subject: RE: [Histonet] p16 To: "'Knutson, Deanne'" , Message-ID: <000001cac055$24c65010$6e52f030$@com> Content-Type: text/plain; charset="US-ASCII" We purchase our p16 through MTM laboratories reference # 9517. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Tuesday, March 09, 2010 5:28 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] p16 Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 10 Mar 2010 09:15:19 -0500 From: "Durden, Kelley" Subject: [Histonet] Masson Trichrome, Alligator Heart Tissue, colors turning out purple To: "histonet@lists.utsouthwestern.edu" Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE591DBE3A@HSC-CMS01.ad.ufl.edu> Content-Type: text/plain; charset="us-ascii" I've been using the Masson Trichrome kit from Richard Allan since 2001 and I've always gotten really great results. It is a little more expensive than using home made reagents but the results are very consistent. Kelley ------------------------------ Message: 18 Date: Wed, 10 Mar 2010 06:44:18 -0800 From: Green JumpyOne Subject: [Histonet] Microwave Tissue Processor Protocols To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I am working on the validation of our microwave processors and have noticed that the tissues are a bit dry. Would anyone be willing to share their protocol timings for their fixation, ethanol, isopropyl and wax impregnation steps? We are using the Histos-5 microwaves from Milestone and are utilizing the 2mm (for the smalls) and the 3mm (for the larger specimens) default program settings. thanks for any help! Michelle ps, my apologies to the board for someone spamming the board using a spoof of my email address. :o( _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/ ------------------------------ Message: 19 Date: Wed, 10 Mar 2010 08:54:11 -0600 From: "Amy Johnson" Subject: [Histonet] HistoDay To: Message-ID: <704247D5A09D004C9E6B115138D1703A1C4B89@hpserv001.aipathology.local> Content-Type: text/plain; charset="us-ascii" Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! ------------------------------ Message: 20 Date: Wed, 10 Mar 2010 08:02:17 -0700 From: "Breeden, Sara" Subject: [Histonet] Histology Professionals Day To: "histonet" Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46F26@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="US-ASCII" If it weren't for a med-tech-turned-pathologist that took me up on my interest in becoming trained in histology (1967), I would not have just marked my 41st year as a histologist. Back then, you just had to have OJT and study independently for your written and practical tests. I had no idea what I was getting into but it was one of the absolute best choices I ever made. To pretend to quote some baseball player, "Histology been berry, berry good to me"! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 21 Date: Wed, 10 Mar 2010 09:07:17 -0600 From: "Nita Searcy" Subject: Re: [Histonet] Histology Professionals Day To: "histonet" , "Sara Breeden" Message-ID: <4B976145.5D38.00EF.0@swmail.sw.org> Content-Type: text/plain; charset="us-ascii" I too "stumbled" into the profession in 1967 and share Sara's sentiments. I sent the following to our senior staff as well as the supervisory group of our laboratory: Today is the first annual National Histotechnology day and is being celebrated by the histology staff by a morning breakfast ( in order that the entire staff can participate) and the wearing of special t-shirts. In spite of almost total anonymity to most of the general public, as well as a considerable amount of health care professionals, these highly skilled individuals play a vital role in healthcare. Wish them congratulations. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Breeden, Sara" 3/10/2010 9:02 AM >>> If it weren't for a med-tech-turned-pathologist that took me up on my interest in becoming trained in histology (1967), I would not have just marked my 41st year as a histologist. Back then, you just had to have OJT and study independently for your written and practical tests. I had no idea what I was getting into but it was one of the absolute best choices I ever made. To pretend to quote some baseball player, "Histology been berry, berry good to me"! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ------------------------------ Message: 22 Date: Wed, 10 Mar 2010 10:09:18 -0500 From: Alyssa Peterson Subject: [Histonet] Happy HPD to our "Unsung Heroes" To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Allied Search Partners wants to wish you a Happy Histology Professionals Day! Histology professionals are the unsung heroes behind the laboratory doors. The patient may not know you but what you all do is the absolute best for them because what you do affects their lives and their loved ones lives. I want to thank each and every one of you for doing what you do behind closed doors away from the surgeons who see the patients and actually get to remove the problem. Thank you for being the Unspoken Heroes!! Please contact us today to inquire about Histology jobs in your area. Alyssa Peterson Director of Recruitment Allied Search Partners Alyssa@alliedsearchpartners.com WWW.ALLIEDSEARCHPARTNERS.COM ------------------------------ Message: 23 Date: Wed, 10 Mar 2010 10:13:35 -0500 From: Merced M Leiker Subject: Re: [Histonet] Need help with FISH staining protocol on frozen tissue sections To: "Shen, Jinhui" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed I have been using IDLabs porcine Y chromosome green label probe on frozen pig tissue with good results most of the time. You say you denature the probe and then hold it at 37oC...I would think that would allow the probe to reanneal to itself since you also hybridize later on at that temp...try following the IDLabs protocol where they say to co-denature the probe and the slide. I do this for my frozen sections and it works. Regards, Merced --On Wednesday, March 10, 2010 12:44 AM -0600 "Shen, Jinhui" wrote: > Hi everyone, > > Has anyone done FISH staining on mice tissue frozen sections for Y > chromosome? I need help with a successful protocol. > > Recently I started to do FISH staining on mice liver frozen sections for > chromosome Y, but got no positive results even on male samples yet. The > probe I used was IDMF1057 Green label Chr Y probe from ID Labs Inc. > (www.idlabs.com). They have a protocol online probably for cultured cells > (http://www.idlabs.com/product/datasheets/IDMF1057.pdf?PHPSESSID=b39f1650 > 7cf76b69495166c651f0b52c) which I haven't tried yet. I followed the > following protocol for frozen sections: > > 1. Fix sections in 4% PFA at 4C for 10 mins, then wash in PBS. > > 2. Treat the sections with 0.01% Pepsin, 37C 10min, wash in PBS. > > 3. Dehydrate the slides using 70% Ethanol (2minsX2), 90% Ethanol (2mins > X2), 100% Ethanol (5 mins X1).Then Age the slides at 65C for one hour. > > 4. Mix the probe and hybridization buffer. Denature the probe by incubate > it at 65C for 10 mins, then hold it at 37C for 30 - 60 mins. > > 5. Denature the sections by incubate them in denaturing solution > (formamide:2XSSC, 70:30) at 75C for 2 mins. > > 6. Quench the slides in 4C 70% Ethanol for 4 mins. Dehydrate the slides > as step 3. > > 7. Apply the probe and hybridize at 37C overnight. > > 8. Immerse the slides in 1XSSC for 5 mins to remove the cover slips. > > 9. Wash slides in stringency solution (formamide:2XSSC, 50:50) at 45C, 5 > mins X2. > > 10. Wash slides in 1XSSC at 45C, 5minsX2. > > 11. Wash slides in detergent wash solution at 45C, 5minX1. > > 12. Rinse slides in PBS. > > 13. Air dry for a couple of minutes and counterstain with DAPI. > > 14. Mount the slides and observe the results or keep in 4C for late check. > > Are there any problems with this procedure? Please help me to check, or > if you could recommend a working protocol you had used, I would really > appreciate. > > Thanks, > > Jinhui > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 24 Date: Wed, 10 Mar 2010 09:13:46 -0600 From: "Beckham, Sharon" Subject: [Histonet] RE: Histology Professionals Day To: "'Breeden, Sara'" , histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Sally, I have pretty much the same story. Back in 1970 I applied for a pathology secretary position and the pathologist asked me if I could sew, which meant he wondered if I could do intricate work with my hands. In my spare time, I trained in histology and took my registry in 1973. It was the best decision I ever made to take him up on his offer. I love, love, love being a histologist and hope that my health remains good so I can do it for many more years to come. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, March 10, 2010 9:02 AM To: histonet Subject: [Histonet] Histology Professionals Day If it weren't for a med-tech-turned-pathologist that took me up on my interest in becoming trained in histology (1967), I would not have just marked my 41st year as a histologist. Back then, you just had to have OJT and study independently for your written and practical tests. I had no idea what I was getting into but it was one of the absolute best choices I ever made. To pretend to quote some baseball player, "Histology been berry, berry good to me"! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 25 Date: Wed, 10 Mar 2010 09:13:45 -0600 From: "Marsh, Nannette" Subject: [Histonet] RE: HistoDay To: 'Amy Johnson' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Here's a quote that we have posted on our Histology event board "Ah, the Professional Hostologist! Walk in awe when you meet one....because there are only a few people who can produce something as beautiful and useful as a slide." by Drs. William Osler and F.E. Mohs----just about says it all, doesn't it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, March 10, 2010 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoDay Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 26 Date: Wed, 10 Mar 2010 09:18:54 -0600 From: "Marsh, Nannette" Subject: [Histonet] RE: HistoDay To: 'Amy Johnson' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Okay--so I don't proof read very well--that was supposed to be 'histologist'--not hostologist in my last post--although there are days when hostility abounds in clinical pathology!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, March 10, 2010 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoDay Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 27 Date: Wed, 10 Mar 2010 08:21:42 -0700 From: "Breeden, Sara" Subject: [Histonet] Histo Professionals Day To: "histonet" Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46F28@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="US-ASCII" Perhaps what each of us ought to do is write a quick line naming the one person that got you involved in histology and how they did that. I'd be willing to gather them and pass them on to NSH so they could post it at the Seattle meeting. If you'd like to do this, send the email to nmhisto@comcast.net and I'll collect. Just an idea... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 11 **************************************** From NMP <@t> stowers.org Wed Mar 10 10:17:58 2010 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Wed Mar 10 10:18:08 2010 Subject: [Histonet] RE: HistoDay In-Reply-To: References: <704247D5A09D004C9E6B115138D1703A1C4B89@hpserv001.aipathology.local> Message-ID: Actually, I also thought about how we hold the tissue blocks 'hostage' until we work our wonders on them :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dianne Holmes Sent: Wednesday, March 10, 2010 9:42 AM To: Histonet Subject: Fwd: [Histonet] RE: HistoDay That's OK cause I re-typed and posted it on my cutting wall along with pics of my favorite slides produced in this lab. Being proud of your work is the best 'teaching tool' I have found. >>> "Marsh, Nannette" 3/10/2010 9:18 AM >>> Okay--so I don't proof read very well--that was supposed to be 'histologist'--not hostologist in my last post--although there are days when hostility abounds in clinical pathology!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, March 10, 2010 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoDay Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Mar 10 10:18:20 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Mar 10 10:18:28 2010 Subject: [Histonet] RE: Histo Professionals Day In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F28@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46F28@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5B1FAD5@LRGHEXVS1.practice.lrgh.org> I got into Histology while I was in the Navy. I was out of MLT school by about three maybe four months, when my wife who was also active duty finally got transferred to the same base as I. There were two openings for her, Blood Bank and Histology. The Navy did not want to put a Med Tech in lowly Histology and we did not want her working for the Officer in charge of Blood Bank. She had previously work for him at another base where his management style got him removed from the lab. So I gave up my position in Hematology and went to Histology, were I met the biggest group of clowns ever. That was 1981. Certified 1985. Took several years off and went into sales. Came back to Histology in 2004 and found that my skills were still there and more important the love for Histology. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbreeden@nmda.nmsu.edu] Sent: Wednesday, March 10, 2010 10:21 AM To: histonet Subject: [Histonet] Histo Professionals Day Perhaps what each of us ought to do is write a quick line naming the one person that got you involved in histology and how they did that. I'd be willing to gather them and pass them on to NSH so they could post it at the Seattle meeting. If you'd like to do this, send the email to nmhisto@comcast.net and I'll collect. Just an idea... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From NMP <@t> stowers.org Wed Mar 10 10:18:55 2010 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Wed Mar 10 10:19:06 2010 Subject: [Histonet] RE: HistoDay In-Reply-To: <5F31F38C96781A4FBE3196EBC22D4780015FB20F@fhosxchmb006.ADVENTISTCORP.NET> References: <704247D5A09D004C9E6B115138D1703A1C4B89@hpserv001.aipathology.local> <5F31F38C96781A4FBE3196EBC22D4780015FB20F@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: Well--there is some hysteria every once in awhile too!!!! ________________________________ From: Bonner, Janet [mailto:Janet.Bonner@FLHOSP.ORG] Sent: Wednesday, March 10, 2010 9:53 AM To: Marsh, Nannette; Amy Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: HistoDay At least it wasn't Hystologist!! @:) Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marsh, Nannette Sent: Wed 3/10/2010 10:18 AM To: 'Amy Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: HistoDay Okay--so I don't proof read very well--that was supposed to be 'histologist'--not hostologist in my last post--although there are days when hostility abounds in clinical pathology!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, March 10, 2010 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoDay Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From billodonnell <@t> catholichealth.net Wed Mar 10 10:33:02 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Mar 10 10:33:18 2010 Subject: [Histonet] RE: Histo Professionals Day In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323D5B1FAD5@LRGHEXVS1.practice.lrgh.org> References: <4D14F0FC9316DD41972D5F03C070908B02E46F28@nmdamailsvr.nmda.ad.nmsu.edu> <38667E7FB77ECD4E91BFAEB8D986386323D5B1FAD5@LRGHEXVS1.practice.lrgh.org> Message-ID: Just a quick note. I too served with Tom, and worked as an MLT in hematology, PM shift. Every test was STAT (and so none were STAT, these were the days most everything was done with minimal automation. We did about 150 CBC's and diffs plus UA, platelets and coags with a staff of 2. And I think we are LEAN now!. And so one evening, when a doctor called for results on a STAT still waiting to be logged in, I asked him "what makes your blankety-blank STAT any more blankity blank STAT than the others"? Presto-chango, the next day I was in histology where the phone never rang! And day time hours! Loved it, primarily because of the clowns Tom mentioned and the almost total lack of automation. Stayed in the field and still enjoy it. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, March 10, 2010 10:18 AM To: Breeden, Sara; histonet Subject: [Histonet] RE: Histo Professionals Day I got into Histology while I was in the Navy. I was out of MLT school by about three maybe four months, when my wife who was also active duty finally got transferred to the same base as I. There were two openings for her, Blood Bank and Histology. The Navy did not want to put a Med Tech in lowly Histology and we did not want her working for the Officer in charge of Blood Bank. She had previously work for him at another base where his management style got him removed from the lab. So I gave up my position in Hematology and went to Histology, were I met the biggest group of clowns ever. That was 1981. Certified 1985. Took several years off and went into sales. Came back to Histology in 2004 and found that my skills were still there and more important the love for Histology. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbreeden@nmda.nmsu.edu] Sent: Wednesday, March 10, 2010 10:21 AM To: histonet Subject: [Histonet] Histo Professionals Day Perhaps what each of us ought to do is write a quick line naming the one person that got you involved in histology and how they did that. I'd be willing to gather them and pass them on to NSH so they could post it at the Seattle meeting. If you'd like to do this, send the email to nmhisto@comcast.net and I'll collect. Just an idea... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Wed Mar 10 10:41:06 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Mar 10 10:41:12 2010 Subject: [Histonet] RE: Histo Professionals Day In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F28@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46F28@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE2242A56C55F@MERCERMAIL.MercerU.local> In 1962 I had completed my first year at Georgia and needed to supplement my tuition since my parents could not foot the whole bill for my next year. I got a job at a furniture store as a bookkeeper/secretary. It took me a week to figure out it was not my future profession. The friend I was rooming with at the time worked in the histology lab at the main hospital. She told me there was an opening and I jumped at the chance to change jobs. I had no idea what histology was, but she said "oh you will like it." She did not stay in the profession and quit 6 months after I started. I stayed, so I kind of stumbled into the histology profession and will have been in it for 48 years in July. I was certified HT(ASCP) in 1964, HTL(ASCP) in 1981 and QIHC in 2000. That's my story and I am sticking to it. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, March 10, 2010 10:22 AM To: histonet Subject: [Histonet] Histo Professionals Day Perhaps what each of us ought to do is write a quick line naming the one person that got you involved in histology and how they did that. I'd be willing to gather them and pass them on to NSH so they could post it at the Seattle meeting. If you'd like to do this, send the email to nmhisto@comcast.net and I'll collect. Just an idea... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Mar 10 10:44:41 2010 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCZVED)) Date: Wed Mar 10 10:46:49 2010 Subject: [Histonet] RE: Histo Professionals Day In-Reply-To: <9BF995BC0E47744E9673A41486E24EE2242A56C55F@MERCERMAIL.MercerU.local> References: <4D14F0FC9316DD41972D5F03C070908B02E46F28@nmdamailsvr.nmda.ad.nmsu.edu> <9BF995BC0E47744E9673A41486E24EE2242A56C55F@MERCERMAIL.MercerU.local> Message-ID: <68510B12184E45498EABD4CB6F3868FE7B53E8@LTA3VS001.ees.hhs.gov> I was in college in Tennessee and my advisor wanted me to make a decision on a career path. I was interested in science but not in being a nurse or a physician. She suggested I look into laboratory sciences: histology, medical technology and cytology. I visited a hospital and toured the labs. I immediately knew when I entered the histo lab (and smelled the xylene) that I was where I needed to be. That was in 1975. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, March 10, 2010 11:41 AM To: Breeden, Sara; histonet Subject: [Histonet] RE: Histo Professionals Day In 1962 I had completed my first year at Georgia and needed to supplement my tuition since my parents could not foot the whole bill for my next year. I got a job at a furniture store as a bookkeeper/secretary. It took me a week to figure out it was not my future profession. The friend I was rooming with at the time worked in the histology lab at the main hospital. She told me there was an opening and I jumped at the chance to change jobs. I had no idea what histology was, but she said "oh you will like it." She did not stay in the profession and quit 6 months after I started. I stayed, so I kind of stumbled into the histology profession and will have been in it for 48 years in July. I was certified HT(ASCP) in 1964, HTL(ASCP) in 1981 and QIHC in 2000. That's my story and I am sticking to it. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, March 10, 2010 10:22 AM To: histonet Subject: [Histonet] Histo Professionals Day Perhaps what each of us ought to do is write a quick line naming the one person that got you involved in histology and how they did that. I'd be willing to gather them and pass them on to NSH so they could post it at the Seattle meeting. If you'd like to do this, send the email to nmhisto@comcast.net and I'll collect. Just an idea... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Mar 10 11:10:28 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Mar 10 11:10:34 2010 Subject: [Histonet] Happy Histotechnology Professionals Day from Pam Barker at RELIA Message-ID: Hi Histonetters!! I wanted to take a moment and say Happy Histotechnology Professionals Day. I really have enjoyed reading about how some of you have gotten into the field. I have to say that I had only heard of the field when I started recruiting in histology 8 years ago. When I went to college at UF in 1978 I wanted to be in a healthcare field but couldn't find one that seemed right for me. I wish my career counselor had known about and told me about histology, I believe that if they had I would be in a lab today. I ended up getting a B.S. in Psychology and became a recruiter. For 18 years I worked in several different areas of recruiting and then in 2002 I spoke to my first histotech and haven't looked back. I want to thank you not only for the job that you do and the lives that you save but also for allowing me to work with you in improving your careers. In all my years of recruiting I have never worked with a more professional, appreciative, honest and truly enjoyable group of people. Kudos to you!! I am curious as to how everyone has celebrated today. I starting celebrating yesterday by contacting each and everyone of my 1000+ client/employers to let them know that today is the inaugural National Histotechnology Professionals Day. Hopefully, if they weren't already doing something to show thier appreciation they are now!! I hope everyone has a great day!! Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From kelleydurden <@t> pathology.ufl.edu Wed Mar 10 11:14:08 2010 From: kelleydurden <@t> pathology.ufl.edu (Durden, Kelley) Date: Wed Mar 10 11:14:57 2010 Subject: [Histonet] Histology Day Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE591DBEF9@HSC-CMS01.ad.ufl.edu> Thanks to the Air Force and AFIP! I entered basic training and did not have a guaranteed job. I listed Histology as the 4th choice in my order of preferences for job placement, having no idea what in the heck histology was. No one there knew what it was either and I thought I'd be doing "normal" lab work with blood, etc Little did I know tissue, slides and stains were my path and I love what I do. Without the military and AFIP's amazing program who knows where I'd be now. Clinical histology is amazing but I am now in research and I've been exposed to a whole new aspect of histology. Thanks to all who are part of this awesome field! Kelley From Allison_Scott <@t> hchd.tmc.edu Wed Mar 10 11:15:41 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Mar 10 11:15:46 2010 Subject: [Histonet] Competency Checkoff Message-ID: <1872B4A455B7974391609AD8034C79FC8BD6EB@LBEXCH01.hchd.local> Does anyone have a competency check off for histology techs that they would be willing to share. Thanks in advance Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From Marilyn.A.Weiss <@t> kp.org Wed Mar 10 12:00:14 2010 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Wed Mar 10 12:00:31 2010 Subject: [Histonet] out of office ON JURY DUTY Message-ID: I will be out of the office starting 03/10/2010 and will not return until 03/11/2010. 10.In my absence please ask for Mary Campbell . If this is urgent you can contact me on my cell phone number 858-472-4266. From fairbairnp <@t> neurosurg.ucsf.edu Wed Mar 10 12:05:33 2010 From: fairbairnp <@t> neurosurg.ucsf.edu (Fairbairn, Patricia) Date: Wed Mar 10 12:06:40 2010 Subject: [Histonet] Microm 550 Repair Message-ID: Does anyone know a good repair guy/company for our Microm 550 cryostat in the San Francisco Bay Area? Thank you, PJ From Valerie.Hannen <@t> parrishmed.com Wed Mar 10 12:26:09 2010 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Wed Mar 10 12:26:14 2010 Subject: [Histonet] FW: Happy Histotechnology Day!! Message-ID: <5680DA93771F0C48954CC8D38425E72401AB3509@ISMAIL.parrishmed.local> ________________________________ From: Hannen, Valerie Sent: Wednesday, March 10, 2010 12:26 PM To: histonet-request@lists.utsouthwestern.edu Subject: Happy Histotechnology Day!! I had graduated from an MLT program in West Virginia in 1983. Moved to Florida in June of 1984. I made my presence known every week for 3 months at the local hospital's HR department...looking for an MLT postion. When a Histology job came open, I was the first one that they thought of (THANK GOD!!). They asked my what my experience with Histology was... well...my only experience was to walk by the department during school, heading to one of the other sections. They said.."With your Lab background, we will give you a try"!! Well, I guess I worked out...I am still here at the same hospital...25 almost 26 years later!! I would not go to any other department now....I LOVE IT in Histoland!!! Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From Janice.Mahoney <@t> alegent.org Wed Mar 10 12:39:29 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Mar 10 12:39:59 2010 Subject: [Histonet] RE: Happy Histotechnology Day!! In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB3509@ISMAIL.parrishmed.local> References: <5680DA93771F0C48954CC8D38425E72401AB3509@ISMAIL.parrishmed.local> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C49F8E@EXCHMBC2.ad.ah.local> I fell in love, quit college (as a music and drama major if you can believe it!) and moved to Omaha. I got a job at a Cancer research center Histo dept, doing filing and some staining. They hired me because I worked in a hospital lab in high school washing glassware. When an opening came up for someone to train, they asked me. I subsequently went back to school, picking up the missing science classes from my transcript and here I am 38 years later, still loving the field of Histology, although on some rare days I do admit I only ACT like it. I still learn every day in this field. It is changing all the time. We are fortunate to have so many dedicated people in our profession. Jan Mahoney Omaha, NE Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From chak_bou <@t> yahoo.com Wed Mar 10 12:43:47 2010 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Wed Mar 10 12:43:50 2010 Subject: [Histonet] IL-17A antibody Message-ID: <777463.38445.qm@web58106.mail.re3.yahoo.com> Hey Histonet, Is anyone using the IL-17A antibody? where did you buy it? where you buy the secondary as well? protocol? Any suggestions will be very much appreciated Thank you Chakib Histology/necropsy Supervisor HTL(ASCP) MIT-DCM From vavalos <@t> allergydermatology.com Wed Mar 10 13:07:34 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Wed Mar 10 13:07:39 2010 Subject: [Histonet] SubX Message-ID: <000001cac084$f1dc4080$d594c180$@com> I am really wanting to end the use of Xylene in our lab and considering the use of SubX from Surgipath/leica. I am currently using Gill III from Surgipath as well. I was wondering if anyone out there is also using SubX and if you would share your staining and processing regimen with me and any feed back on using SubX. We would be staining/processing derm only. Thank you very much! V.Avalos ADS, INC Fax 602-277-2134 From brian <@t> prometheushealthcare.com Wed Mar 10 13:32:45 2010 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Wed Mar 10 13:32:51 2010 Subject: [Histonet] New York Job Openings- Histology Technician / Technologist / Supervisor Message-ID: <031c01cac088$75b03d50$6110b7f0$@com> Prometheus Healthcare, New York's # 1 Recruiter for Laboratory professionals, is currently seeking technicians and technologists for numerous histology opportunities throughout New York City and the surrounding areas. Shifts vary from day, evening to night and opportunities are available within grossing, embedding, cutting, staining, and IHC. Supervisor positions are also available. The facilities we work with include some of the most prestigious hospitals, reference labs and biotech companies. Depending on position and location, sign on bonuses and generous shift differentials are offered as well! Please contact us today at 301-693-9057 to discuss our current openings. You can email a resume or visit our website at www.prometheushealthcare.com ****Also be sure to join us on Twitter to stay up to date on our newest lab positions**** http://twitter.com/PrometheusBlog Requirements Must have current NY technician or technologist license ASCP preferred At least 1 year histology experience Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From vgrover <@t> polysciences.com Wed Mar 10 14:45:45 2010 From: vgrover <@t> polysciences.com (Valantou Grover) Date: Wed Mar 10 14:45:58 2010 Subject: [Histonet] Histotechnology Day Message-ID: <169CD52CECC24D22B9ACC0CB16B53346@USWARD13ZFB71> Happy Histotechnology Day to everyone out there in Histoland, whether that be clinical, research, veterinary, industry, or all of the above. Since we are sharing how we all got started in Histotechnology, I remember having a Premed/Biochemistry degree in 1996,(what do you do with that?) shortly thereafter, I fell into Histotechnology by going back to Youngstown State University. All I remember was taking the Medical Technology class in Hematology and waking up after fainting from one of my classmates taking my blood for lab that day (blood counts and hematocrits). I do not know if it was seeing the blood in the tube(my blood- other people's blood doesn't phase me)or the classmate trying to take my blood for over 30 minutes, but I said ew! Blood! The second class was a diagnostic microbiology class and my first gram stain ever. I think that is when I was hooked, I liked staining, I liked everything about Histotechnology so thanks to the good folks at Compunet Clinical Labs, Dayton OH and some very dear angels named Alice Trent, Denise Gerard, Dr. Joan Boyd, Dr. Maria Delost, Dr. Daniel Hood, Dr. Atef Shrit, and Joseph Mistovich. I was off to finish my practical year in 1997-1998. Compunet Clinical Labs gave me the opportunity by paying for my clinical year tuition, room and board, giving me a job after rotation/clinical hours, and a guaranteed position for me for at least two years. I was just 19 at the time. It was an amazing experience and I would not change one thing or one person that I met along the way. Histotechnology has opened the path to me becoming a PA, working at Penn in the IHC/Molecular Pathology department and now at Polysciences, Inc. Not to mention my stent as a travel tech, (thanks Jeanie Nolle who introduced me to the wild and wonderful Alaska). I just received a card and a bouquet from my co workers here at Polysciences, Inc. for my first Histotechnology Day ever, and I would like to thank them and all the people that I have ever crossed paths with in the Histotechnology field, including some of the dearest friends I have ever made along my way here and you know who you are. Happy Histotechnologists' Day!!!! Sincerely, Valantou Valantou Grover, HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-343-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 From Wanda.Smith <@t> HCAhealthcare.com Wed Mar 10 15:34:46 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Mar 10 15:34:42 2010 Subject: [Histonet] SC Governor Signs Proclamation Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1386C19AF2@NADCWPMSGCMS03.hca.corpad.net> Good Afternoon Everyone, Vinnie, Chad McMahan and I have just returned from the SC State capital for Governor Sanford to sign a proclamation for Histotechnology Professional's Day. It was a rewarding experience having our Governor acknowledge our profession with the signing of the proclamation. I hope everyone had a GREAT 1st annual HPD!!!!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From POWELL_SA <@t> mercer.edu Wed Mar 10 15:58:36 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Mar 10 15:58:44 2010 Subject: [Histonet] RE: SC Governor Signs Proclamation In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1386C19AF2@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1386C19AF2@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <9BF995BC0E47744E9673A41486E24EE2242A56C8AE@MERCERMAIL.MercerU.local> Way to go South Carolina. Congratulations. Hope your day was as good as mine. Low key but hey, I am the only one here. Got flowers from my Pathologists. We can start planning for next year. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Wednesday, March 10, 2010 4:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SC Governor Signs Proclamation Good Afternoon Everyone, Vinnie, Chad McMahan and I have just returned from the SC State capital for Governor Sanford to sign a proclamation for Histotechnology Professional's Day. It was a rewarding experience having our Governor acknowledge our profession with the signing of the proclamation. I hope everyone had a GREAT 1st annual HPD!!!!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From veronicadfw <@t> hotmail.com Wed Mar 10 17:17:32 2010 From: veronicadfw <@t> hotmail.com (Veronica Coker) Date: Wed Mar 10 17:17:37 2010 Subject: [Histonet] Where did Dezna C. Sheehan practice? Message-ID: Happy Histotechnology Professionals Day! One of my Professors told me that Sheehan(of Theory and Practice of Histotechnology-by Dezna C. Sheehan, Barbara B. Hrapchak) practiced her work in Philadelphia. I have interest in this for I am originally from Philadelphia. Does anyone know where she did most of her work? Thank You, -Veronica Coker _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/ From rjbuesa <@t> yahoo.com Wed Mar 10 17:17:32 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 10 17:17:39 2010 Subject: [Histonet] Competency Checkoff In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD6EB@LBEXCH01.hchd.local> Message-ID: <760807.62887.qm@web65710.mail.ac4.yahoo.com> For which tasks? Ren? J. --- On Wed, 3/10/10, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Competency Checkoff To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 10, 2010, 12:15 PM Does anyone have a competency check off for histology techs that they would be willing to share.? Thanks in advance Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DDittus787 <@t> aol.com Wed Mar 10 17:37:50 2010 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Wed Mar 10 17:38:17 2010 Subject: [Histonet] Where did Dezna C. Sheehan practice? Message-ID: <363ba.6dc172fa.38c9874e@aol.com> Veronica I am pretty sure it was University of Penna. , anyone out there know different? Dana In a message dated 3/10/2010 6:18:02 P.M. Eastern Standard Time, veronicadfw@hotmail.com writes: Happy Histotechnology Professionals Day! One of my Professors told me that Sheehan(of Theory and Practice of Histotechnology-by Dezna C. Sheehan, Barbara B. Hrapchak) practiced her work in Philadelphia. I have interest in this for I am originally from Philadelphia. Does anyone know where she did most of her work? Thank You, -Veronica Coker _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/____________________________ ___________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cscampbe <@t> uci.edu Wed Mar 10 18:20:41 2010 From: cscampbe <@t> uci.edu (cscampbe@uci.edu) Date: Wed Mar 10 18:20:45 2010 Subject: [Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent Message-ID: Hi Histonet, I am performing the trichrome stain on cardiac tissue. The procedure calls for Weigert's iron hematoxylin stain or equivalent. Would Harris hematoxylin work as well. It's what I have readily available at the lab. The instructions for preparing the Weigert stain involves mixing fresh solutions from two stock solutions - but the staining process of all the slides will take weeks. How long does the solution stay "fresh?" Thanks! -Colin From STACEY.LANGENBERG <@t> UCDENVER.EDU Wed Mar 10 18:24:54 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Wed Mar 10 18:25:47 2010 Subject: [Histonet] RE: SC Governor Signs Proclamation In-Reply-To: <9BF995BC0E47744E9673A41486E24EE2242A56C8AE@MERCERMAIL.MercerU.local> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1386C19AF2@NADCWPMSGCMS03.hca.corpad.net><9BF995BC0E47744E9673A41486E24EE2242A56C8AE@MERCERMAIL.MercerU.local> Message-ID: <568915435.622417.1268267140206.JavaMail.rim@bda2340.bisx.prod.on.blackberry> Colorados Governor Bill Ritter also signed a Proclamation Sent via BlackBerry from T-Mobile -----Original Message----- From: "Shirley A. Powell" Date: Wed, 10 Mar 2010 14:58:36 To: Wanda.Smith@HCAhealthcare.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: SC Governor Signs Proclamation Way to go South Carolina. Congratulations. Hope your day was as good as mine. Low key but hey, I am the only one here. Got flowers from my Pathologists. We can start planning for next year. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Wednesday, March 10, 2010 4:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SC Governor Signs Proclamation Good Afternoon Everyone, Vinnie, Chad McMahan and I have just returned from the SC State capital for Governor Sanford to sign a proclamation for Histotechnology Professional's Day. It was a rewarding experience having our Governor acknowledge our profession with the signing of the proclamation. I hope everyone had a GREAT 1st annual HPD!!!!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Mar 10 18:43:31 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Mar 10 18:43:42 2010 Subject: [Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent In-Reply-To: Message-ID: Colin, Rather than Weigert's Hx you can use the Celestin Blue Harris Hx sequence. Stain 5minutes in Celestine Blue (0.5% Celestine Blue (CI 51050) in 5% iron alum (ferric ammonium sulphate)), followed by Harris Hx 5 minutes, contine Masson Staining. See: Henwood T, Llewellyn B, Montgomery I, Nader A, Rittman B R (2007) "Celestine blue and hemalum for staining nuclei" Biotechnic & Histochemistry 82(3): 167-168. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cscampbe@uci.edu Sent: Thursday, 11 March 2010 11:21 AM To: HistoNet Subject: [Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent Hi Histonet, I am performing the trichrome stain on cardiac tissue. The procedure calls for Weigert's iron hematoxylin stain or equivalent. Would Harris hematoxylin work as well. It's what I have readily available at the lab. The instructions for preparing the Weigert stain involves mixing fresh solutions from two stock solutions - but the staining process of all the slides will take weeks. How long does the solution stay "fresh?" Thanks! -Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From POWELL_SA <@t> mercer.edu Wed Mar 10 18:49:02 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Mar 10 18:49:26 2010 Subject: [Histonet] RE: SC Governor Signs Proclamation In-Reply-To: <568915435.622417.1268267140206.JavaMail.rim@bda2340.bisx.prod.on.blackberry> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1386C19AF2@NADCWPMSGCMS03.hca.corpad.net> <9BF995BC0E47744E9673A41486E24EE2242A56C8AE@MERCERMAIL.MercerU.local>, <568915435.622417.1268267140206.JavaMail.rim@bda2340.bisx.prod.on.blackberry> Message-ID: <9BF995BC0E47744E9673A41486E24EE22429A7E249@MERCERMAIL.MercerU.local> Congratulations to you as well. Gee this is great. Shirley ________________________________________ From: Langenberg, Stacey [STACEY.LANGENBERG@UCDENVER.EDU] Sent: Wednesday, March 10, 2010 7:24 PM To: Shirley A. Powell; histonet-bounces@lists.utsouthwestern.edu; Wanda.Smith@HCAhealthcare.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: SC Governor Signs Proclamation Colorados Governor Bill Ritter also signed a Proclamation Sent via BlackBerry from T-Mobile -----Original Message----- From: "Shirley A. Powell" Date: Wed, 10 Mar 2010 14:58:36 To: Wanda.Smith@HCAhealthcare.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: SC Governor Signs Proclamation Way to go South Carolina. Congratulations. Hope your day was as good as mine. Low key but hey, I am the only one here. Got flowers from my Pathologists. We can start planning for next year. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Wednesday, March 10, 2010 4:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SC Governor Signs Proclamation Good Afternoon Everyone, Vinnie, Chad McMahan and I have just returned from the SC State capital for Governor Sanford to sign a proclamation for Histotechnology Professional's Day. It was a rewarding experience having our Governor acknowledge our profession with the signing of the proclamation. I hope everyone had a GREAT 1st annual HPD!!!!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Mar 10 20:52:11 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Mar 10 20:52:16 2010 Subject: [Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent In-Reply-To: References: Message-ID: No. The PTA or PMA used in Masson's method is too acidic and would remove Harris's or any other haemalum. An iron-haematoxylin such as Weigert's or Lillie's gives an acid-resistant nuclear stain that is progressive and almost black. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: cscampbe@uci.edu Date: Wednesday, March 10, 2010 19:21 Subject: [Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent To: HistoNet > Hi Histonet, > > I am performing the trichrome stain on cardiac tissue. The > procedure calls > for Weigert's iron hematoxylin stain or equivalent. Would Harris > hematoxylin work as well. It's what I have readily available at > the lab. > > The instructions for preparing the Weigert stain involves mixing fresh > solutions from two stock solutions - but the staining process of > all the > slides will take weeks. How long does the solution stay "fresh?" > > Thanks! > -Colin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Mar 10 21:04:41 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Mar 10 21:04:45 2010 Subject: [Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent Message-ID: No. The PTA or PMA used in Masson's method is too acidic and would remove Harris's or any other haemalum. An iron-haematoxylin such as Weigert's or Lillie's gives an acid-resistant nuclear stain that is progressive and almost black. The stock solutions for Weigert's are good for many years. The mixture keeps for 3 or 4 days at room temp or 10-14 days in a fridge. Lillie's modification is more stable - OK for 2-3 weeks at room temperature. These iron-haematoxylins are ready to use as soon as they are mixed. No "ripening" is needed because the ferric chloride serves as both the oxidizing agent and the metal salt that forms a black complex with haematein. Deterioration with time is due to over-oxidation, converting haematein to other compounds. If the nuclei have a brownish shade instead of clean blue-black it's time to make a new working solution. John Kiernan Anatomy, UWO London, Canada = = = > ----- Original Message ----- > From: cscampbe@uci.edu > Date: Wednesday, March 10, 2010 19:21 > Subject: [Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent > To: HistoNet > > > Hi Histonet, > > > > I am performing the trichrome stain on cardiac tissue. The > > procedure calls > > for Weigert's iron hematoxylin stain or equivalent. Would Harris > > hematoxylin work as well. It's what I have readily available at > > the lab. > > > > The instructions for preparing the Weigert stain involves mixing fresh > > solutions from two stock solutions - but the staining process of > > all the > > slides will take weeks. How long does the solution stay "fresh?" > > > > Thanks! > > -Colin > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.h.reilly <@t> clinmed.gla.ac.uk Thu Mar 11 03:02:50 2010 From: j.h.reilly <@t> clinmed.gla.ac.uk (Jim Reilly) Date: Thu Mar 11 03:02:55 2010 Subject: [Histonet] IL17A Message-ID: Hello Chakib I can recommend this paper: Cutting Edge: Mast Cells Express IL-17A in Rheumatoid Arthritis Synovium. Axel J Hueber, Darren L. Asquith, Ashley M. Miller, Jim Reilly, Shauna Kerr, Jan Leipe, Alirio J. Melendez, Iain B. McInnes. J Immunol. 2010, 184:000-000 ahead of print. Jim Reilly From jcbook <@t> gmail.com Thu Mar 11 05:36:35 2010 From: jcbook <@t> gmail.com (J C) Date: Thu Mar 11 05:36:39 2010 Subject: [Histonet] Need help with FISH staining protocol on Message-ID: <52cb0f131003110336r7f617f5hbef28dc6fea87f9d@mail.gmail.com> Dear Jinhui! We tried for a long time to perform SRY FISH for rats, paraffin, frosen nothinhg worked. I know other people that did not suceeded on other animals, I do not know anybody that could do it exept of somebody in German, that worked with special plasmid for rat. There are not repeated works in pubmed. I doubt that this method works good for rats or mices, but Cumbio has a kit, maybe it will work. Regards, Julie From MAUGER <@t> email.chop.edu Thu Mar 11 06:33:59 2010 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Thu Mar 11 06:34:24 2010 Subject: [Histonet] Where did Dezna C. Sheehan practice? In-Reply-To: References: Message-ID: <443F5B475A9BF647AB962E834884EBAD2731A4E393@EX7CCRPW03V1.chop.edu> Hi All, Dezna Sheehan worked at the Hospital of the University of Penn. for many years. She ran a school for Histotechnology which I and many of my friends and colleagues were lucky to have gone to. We are proud to continue her push for the advancement of our field. Happy Histotechnology Professionals Day!! Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Veronica Coker [veronicadfw@hotmail.com] Sent: Wednesday, March 10, 2010 6:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where did Dezna C. Sheehan practice? Happy Histotechnology Professionals Day! One of my Professors told me that Sheehan(of Theory and Practice of Histotechnology-by Dezna C. Sheehan, Barbara B. Hrapchak) practiced her work in Philadelphia. I have interest in this for I am originally from Philadelphia. Does anyone know where she did most of her work? Thank You, -Veronica Coker _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Thu Mar 11 06:48:58 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Mar 11 06:49:03 2010 Subject: [Histonet] opinion on cryojane Message-ID: <880170.71788.qm@web50308.mail.re2.yahoo.com> Hi?All, What is everyone's opinion on the cryojane?? Do you think it is worth the money? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From sbreeden <@t> nmda.nmsu.edu Thu Mar 11 07:09:11 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Mar 11 07:09:16 2010 Subject: [Histonet] Histo Stories Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> Thanks to everyone that sent their Story of How I Ended Up Doing This Histology Thing! I have gotten 50 or more replies! The one thing that strikes me is how many of us went into this profession without a clue! With all the opportunities to recruit future histologists, this Histology Day idea is a good start. On the original subject, I'm planning to make one document out of all the replies and - WITH PERMISSION - attach your name to the answers. If you do NOT want your submission listed because you want to remain anonymous, you must let me know ASAP. Send to: nmhisto@comcast.net. Thanks for your stories! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From NMP <@t> stowers.org Thu Mar 11 07:21:34 2010 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Thu Mar 11 07:21:46 2010 Subject: [Histonet] RE: SC Governor Signs Proclamation In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1386C19AF2@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1386C19AF2@NADCWPMSGCMS03.hca.corpad.net> Message-ID: The mayor of Kansas City also signed a proclamation stating March 10th is Histotechnology Professional's Day :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Wednesday, March 10, 2010 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SC Governor Signs Proclamation Good Afternoon Everyone, Vinnie, Chad McMahan and I have just returned from the SC State capital for Governor Sanford to sign a proclamation for Histotechnology Professional's Day. It was a rewarding experience having our Governor acknowledge our profession with the signing of the proclamation. I hope everyone had a GREAT 1st annual HPD!!!!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Thu Mar 11 08:46:31 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Mar 11 08:46:48 2010 Subject: [Histonet] Formalin vs alcoholic formalin In-Reply-To: <77887EF5-EBDE-43F8-9040-F1AD1ED8E98A@mimectl> References: <77887EF5-EBDE-43F8-9040-F1AD1ED8E98A@mimectl> Message-ID: Help! I think I know the answer, but need some rapid clarification. Is an alcohol-formalin fixative acceptable for use in breast tissue, or does it need to be an aqueous 10% NBF? William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ________________________________ From MLunetta <@t> luhcares.org Thu Mar 11 08:50:30 2010 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Thu Mar 11 08:50:46 2010 Subject: [Histonet] Excelsior Vs. VIP 6 Message-ID: <4B98A0C6020000A800040A69@ns.luhcares.org> Hello to the World Wide Histo's, We are looking at getting a new processor and was wondering what everyones feelings were on the Excelsior from Thermo vs the VIP 6 from Sakura. We have a VIP5 right now and it is a delight. The demo of the Excelsior was very impressive. Thanks for your thoughts. Matt HT (ASCP) Longmont United Hospital Longmont, Colorado From algranth <@t> email.arizona.edu Thu Mar 11 08:59:24 2010 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Mar 11 08:59:43 2010 Subject: [Histonet] Histo Stories In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Sally, I didn't get a chance to answer yesterday - I went to a high school and spoke about my favorite topic - histotechnology! Anyway, I started out in microbiology and then worked in a doctor's office lab doing routine chemistry and hematology and when we moved to a small town in Iowa the only position in the lab that became available was in histology. I was playing bridge with the histotech and he mentioned the opening so I went in and interviewed, got the job and never have looked back. So I sort of fell into this profession as did many others. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Mar 11, 2010, at 6:09 AM, Breeden, Sara wrote: > Thanks to everyone that sent their Story of How I Ended Up Doing This > Histology Thing! I have gotten 50 or more replies! The one thing > that > strikes me is how many of us went into this profession without a clue! > With all the opportunities to recruit future histologists, this > Histology Day idea is a good start. On the original subject, I'm > planning to make one document out of all the replies and - WITH > PERMISSION - attach your name to the answers. If you do NOT want your > submission listed because you want to remain anonymous, you must let > me > know ASAP. Send to: nmhisto@comcast.net. Thanks for your stories! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Thu Mar 11 09:00:09 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Mar 11 09:00:16 2010 Subject: [Histonet] RELIA Histology Jobs Alert - Hot New Histology Jobs Histotechs, Managers, PA's Message-ID: Hi Histonetters!! I hope everyone is having a great day! I wanted to take a moment and tell you about some new opportunities I am working with. Here is a list of my current openings. All of these positions are fulltime permanent positions and my clients offer excellent salaries, benefits and relocation assistance. HISTOLOGY/PATHOLOGY MANAGEMENT AZ - Phoenix Histology Lab Manager VA - Richmond Histology Manager NY - Long Island Histology Manager WA-Spokane-Histology Supervisor-Hospital CA - Central CA - Pathology Supervisor NV - Las Vegas Histology Supervisor HISTOTECHS FL-Miami Histotechnologist needed for growing private lab TX - Austin Night Shift Grossing Histotech excellent shift diff TX - Corpus Christi - Histotechnician day shift private lab MA - Cape Cod Immunohistochemistry Specialist MA - Cape Cod Histotechnologist/Histotechnician GA - Atlanta area Histotechs needed all shifts GA - Atlanta area Immunohistochemistry Specialist 2p-10p GA- Atlanta Grossing Histotechnologist Night Shift Great Shift diff NY-Orange/Rockland County Brand New Lab NYS license/Elig brand new lab 2nd shift. CA-Los Angeles Histotechnologist afternoon shift NV - Las Vegas Histotechnician/Histotechnologist PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC - Charlotte PA grad from NAACLES program required FL- Miami Growing private lab If you or anyone you know might be interested in any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia www.twitter.com/pamatrelia From JWeems <@t> sjha.org Thu Mar 11 09:29:38 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Mar 11 09:29:44 2010 Subject: [Histonet] Histo Stories In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <27648A6C5BC9B145813DF5182F83AB450756E1@ITSSSXM01V1.one.ads.che.org> One more story!! >From 2 yrs of age I had wanted to be a nurse. Love/marriage got in the way and I didn't do the nursing program but started out for a degree in Biology. Then dropped out of school and obtained PhT (putting hubby through). Applied at the Youth Opportunity Center in Knoxville, TN in the fall of 1966. Received a call asking if I would be interested in training in Histology. I had not a clue, but said yes. Trained at UT Hospital and was registered in 1968. Took enough classes here and there from TN to Alaska to NM to have enough requirements to sit for HTL and obtained my degree in Business Management from Tusculum College Adult Studies. May Momma was so proud...only took me 30 years to finish my degree! :>) And here I am...still at it. Love this site and feel so connected to you all through it. Very grateful to Linda and Herb for their foresight to originate it and their patience with us. Thanks for your vast wisdom. I am amazed at what some of you do and wish I had time to read in detail and learn it all. I would like to sit at your feet and soak it all up!! Blessings to everyone, Joyce Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From greenjumpyone <@t> hotmail.com Thu Mar 11 09:36:56 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Thu Mar 11 09:37:04 2010 Subject: [Histonet] Histology Stories In-Reply-To: References: Message-ID: I am really enjoying reading these "start-up" stories. :o) If you don't mind indulging me, I'll share mine too! I had just graduated from college with a BS in Biology when I landed a job as a cancer research assistant. Well, it turns out that portion of lab just wasn't for me! I left that department and worked in another area of the lab. Then the histotech quit. They looked at me, said "you have a degree, you can do this". I had no idea what histology was other than looking at the cells under the microscope! They had no problem with that and they set about teaching me how to use the equipment. I had no theory, no understanding of *why* I was doing any of what I was doing, I just learned the practical side histology: process, embed, cut, stain. I learned how to cut with my knees in a cupboard (they didn't have a proper desk for me) and not with forceps or brushes, but with chop sticks! You see, the first person to introduce me to Histology was a graduate student, from Japan, who was doing an internship at our facility. :o) I stayed at that position for about 1.5 years, but absolutely had to leave it because I developed a very severe allergy and asthma to the rats we were doing our research on. I was offered a position in (what was then) the largest private lab in MI. My true mentor, Glenda, taught me anything and everything I know about Histology. She helped me study for the HT exam, spending countless hours of her own time helping me learn. Thanks to her, I passed the HT the first time around! Later, she assisted me in studying for the HTL exam which I also passed! Had it not been for her kindness and guidance, I'm not so sure I would have succeeded. :o) THANKS GLENDA!! Glenda had no formal education after high school - everything she learned was via on the job training. I will say, she learned very, very well! :o) So much so that she now has a QIHC after her name. And now where are we? I am trying to figure out just how to have our very own Histotech school here at my hospital. We are affiliated with another school (with me as the mentor), but I am thinking it would be nice to run our own. See? What goes around, comes around! It's amazing just how far we have come! From stropping our own knifes, to disposible ones; from maintaining our (and in some cases, making new parts!) microtomes to having maintenance free ones; from all the manual staining to the automated; and now microwave technology for the processing. Yes, indeedy, we sure have evolved! Gone are the days of grabbing someone from the lab and saying "you can be a histotech"!! We have to be formally educated now! :o) I love my job! Michelle _________________________________________________________________ Hotmail: Trusted email with Microsoft?s powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/ From Laura.Miller <@t> leica-microsystems.com Thu Mar 11 10:01:17 2010 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Thu Mar 11 10:01:22 2010 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 03/11/2010 and will not return until 04/05/2010. I am on medical leave until April 5. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From b-frederick <@t> northwestern.edu Thu Mar 11 10:09:37 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 11 10:09:55 2010 Subject: [Histonet] Histology Stories In-Reply-To: Message-ID: <18639C6EABE44E33A3CE9DABF37687D6@lurie.northwestern.edu> Makes my story kind of dull! I was in college in nursing then went to Allied Health (MT, more or less) but had no idea histo existed until I got to this point. I thought it was more interesting than running blood and urine samples and went for it. Histo school and then the HTL. That was 26 years ago (eek). I worked most of my years in a hospital setting, now I work in a core lab and we do animal translational studies for researchers here at NU as well as being the reference lab for ECOG, who are one of the biggest cooperative groups in the country running cancer clinical trials. We get all the blocks and or slides for these trials here at NU from the US as well as other countries. Makes for a varied and interesting job. And yes, I do remember the days of regular knives and making up my own Schiff's! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Thursday, March 11, 2010 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Stories I am really enjoying reading these "start-up" stories. :o) If you don't mind indulging me, I'll share mine too! I had just graduated from college with a BS in Biology when I landed a job as a cancer research assistant. Well, it turns out that portion of lab just wasn't for me! I left that department and worked in another area of the lab. Then the histotech quit. They looked at me, said "you have a degree, you can do this". I had no idea what histology was other than looking at the cells under the microscope! They had no problem with that and they set about teaching me how to use the equipment. I had no theory, no understanding of *why* I was doing any of what I was doing, I just learned the practical side histology: process, embed, cut, stain. I learned how to cut with my knees in a cupboard (they didn't have a proper desk for me) and not with forceps or brushes, but with chop sticks! You see, the first person to introduce me to Histology was a graduate student, from Japan, who was doing an internship at our facility. :o) I stayed at that position for about 1.5 years, but absolutely had to leave it because I developed a very severe allergy and asthma to the rats we were doing our research on. I was offered a position in (what was then) the largest private lab in MI. My true mentor, Glenda, taught me anything and everything I know about Histology. She helped me study for the HT exam, spending countless hours of her own time helping me learn. Thanks to her, I passed the HT the first time around! Later, she assisted me in studying for the HTL exam which I also passed! Had it not been for her kindness and guidance, I'm not so sure I would have succeeded. :o) THANKS GLENDA!! Glenda had no formal education after high school - everything she learned was via on the job training. I will say, she learned very, very well! :o) So much so that she now has a QIHC after her name. And now where are we? I am trying to figure out just how to have our very own Histotech school here at my hospital. We are affiliated with another school (with me as the mentor), but I am thinking it would be nice to run our own. See? What goes around, comes around! It's amazing just how far we have come! From stropping our own knifes, to disposible ones; from maintaining our (and in some cases, making new parts!) microtomes to having maintenance free ones; from all the manual staining to the automated; and now microwave technology for the processing. Yes, indeedy, we sure have evolved! Gone are the days of grabbing someone from the lab and saying "you can be a histotech"!! We have to be formally educated now! :o) I love my job! Michelle _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/____________________________ ___________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From zerfasp <@t> ors.od.nih.gov Thu Mar 11 10:25:16 2010 From: zerfasp <@t> ors.od.nih.gov (Zerfas, Patricia (NIH/OD/ORS) [E]) Date: Thu Mar 11 10:25:25 2010 Subject: [Histonet] H. pylori on Rhesus macaque tissue Message-ID: Dear List servers, Did anyone try this antibody on Rhesus macaque tissue? Do you have a protocol? How were the results? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 From Jessica.Vacca <@t> HCAhealthcare.com Thu Mar 11 10:49:08 2010 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Thu Mar 11 10:49:15 2010 Subject: [Histonet] Histology Stories Message-ID: <938D716CD445614ABBB817517557B6F4E30EBB84@NADCWPMSGCMS09.hca.corpad.net> I was introduced the color world of Histology, when I was about 7 or 8. I saw my first leg being grossed. I was the cool kid in elementary school that during show and tell, would bring in a section of brain or perhaps an embryo floating in formalin. I worked my summers filing blocks and slides (Not to worry I understood the importance of numerical order!), and as I got older would work my summers as a lab aide. After high school, and very undecided in which direction my life should go, the Histology Supervisor had encouraged as she did all her lab aides and others she felt needed to add their mark in this profession into this career. She had a histology program (at the time when it was OJT) and she would have 3 students at a time. We would work nights assisting with gross, and mornings in class. She would give us weekly exams and instill in us the importance of the profession. The majority of her students that she had taught have moved on to become supervisors and charge techs. I have to say that I come from a "family" of histologists. I was very fortunate that this woman who had an interest in my future not just in me as a person but as her daughter. You see, this supervisor was my mother, and I will forever be grateful to her for introducing me to this field. Her name is Sofia Roberts and I'm sure that there are many members that know her. So to her I say "Happy Histologist Professional Day"! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr Brandon Fl 33511 (813) 571-6410 or ext 2454 (813) 571-5169 FAX ? From Timothy.Morken <@t> ucsfmedctr.org Thu Mar 11 11:04:39 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Mar 11 11:04:52 2010 Subject: [Histonet] RE: Histo Stories In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1AAF670737F193429070841C6B2ADD4C013AAF730E@EXMBMCB15.ucsfmedicalcenter.org> Sally, I started in Electron Microscopy after taking a two-year EM course at Delta College in Stockton, CA. I had never seen a histology lab before starting work at Valley Medical Center in Fresno, CA. I gradually started helping out in histology when I didn't have enough EM work to keep me busy. I started by coverslipping, worked into special stains, then cutting (though I never really cut very much). I started the immunohistochemistry service there in 1983. The guys in our lab (literally - we had four men, no women!) started a study group to work on the HT test and we took that in 1989, all passing. Later I took the HTL and passed that. After I left Valley Medical center ion 1993 I worked primarily in immunohhistochemisty and managed histology labs. I went to Saudi Arabia for 5 years, then Centers for Disease Control in Atlanta, into industry with Lab Vision (IHC Autostainer and reagents - a very eye-opening education on the vendor side of things!), and now back to histology at University of California, San Francisco Medical Center. Histology is so varied and hands-on that it is always interesting. There is always something new coming along. I started in histology just before the IHC revolution, when virtually everything done in histology was based on 50 to 100-year-old technology. Now we are at the forefront right along with every other lab discipline. It has been a good ride all the way. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, March 11, 2010 5:09 AM To: histonet Subject: [Histonet] Histo Stories Thanks to everyone that sent their Story of How I Ended Up Doing This Histology Thing! I have gotten 50 or more replies! The one thing that strikes me is how many of us went into this profession without a clue! With all the opportunities to recruit future histologists, this Histology Day idea is a good start. On the original subject, I'm planning to make one document out of all the replies and - WITH PERMISSION - attach your name to the answers. If you do NOT want your submission listed because you want to remain anonymous, you must let me know ASAP. Send to: nmhisto@comcast.net. Thanks for your stories! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SLB <@t> stowers.org Thu Mar 11 11:05:06 2010 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Thu Mar 11 11:05:20 2010 Subject: [Histonet] RE: Histology Stories In-Reply-To: <938D716CD445614ABBB817517557B6F4E30EBB84@NADCWPMSGCMS09.hca.corpad.net> References: <938D716CD445614ABBB817517557B6F4E30EBB84@NADCWPMSGCMS09.hca.corpad.net> Message-ID: Your introduction into histology brought back a very funny memory for me. My kids are in their 30's now, but when my daughter was 7 or 8 she also saw her first grossing specimen which was also a leg. She tried to talk about it in show and tell and her teacher made her stop. She didn't want to hear about it and my daughter was so excited about being able to share the information with her classmates. When my son was 14 or 15 he wanted to see a brain and we happened to have one from an autopsy case. I took it out and he got one whiff of the formalin and said "Mom, no wonder you are so weird, having to smell that stuff everyday". No one can appreciate what we do quite like our children!! Jessica, that was a really cool story about your Mom introducing you to histology. It brought a tear to my eye! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Thursday, March 11, 2010 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Stories I was introduced the color world of Histology, when I was about 7 or 8. I saw my first leg being grossed. I was the cool kid in elementary school that during show and tell, would bring in a section of brain or perhaps an embryo floating in formalin. I worked my summers filing blocks and slides (Not to worry I understood the importance of numerical order!), and as I got older would work my summers as a lab aide. After high school, and very undecided in which direction my life should go, the Histology Supervisor had encouraged as she did all her lab aides and others she felt needed to add their mark in this profession into this career. She had a histology program (at the time when it was OJT) and she would have 3 students at a time. We would work nights assisting with gross, and mornings in class. She would give us weekly exams and instill in us the importance of the profession. The majority of her students that she had taught have moved on to become supervisors and charge techs. I have to say that I come from a "family" of histologists. I was very fortunate that this woman who had an interest in my future not just in me as a person but as her daughter. You see, this supervisor was my mother, and I will forever be grateful to her for introducing me to this field. Her name is Sofia Roberts and I'm sure that there are many members that know her. So to her I say "Happy Histologist Professional Day"! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr Brandon Fl 33511 (813) 571-6410 or ext 2454 (813) 571-5169 FAX ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Mar 11 11:09:06 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Mar 11 11:09:17 2010 Subject: [Histonet] RE: Histology Stories In-Reply-To: <938D716CD445614ABBB817517557B6F4E30EBB84@NADCWPMSGCMS09.hca.corpad.net> References: <938D716CD445614ABBB817517557B6F4E30EBB84@NADCWPMSGCMS09.hca.corpad.net> Message-ID: <1AAF670737F193429070841C6B2ADD4C013AAF7312@EXMBMCB15.ucsfmedicalcenter.org> " I was introduced the color world of Histology, when I was about 7 or 8. " That reminds me of when I first brought my daughter to the lab. She was about 6 and one of our pathologists was there. He asked if she wanted to see anything and she piped right up "I want to see some brains!" So he took a whole brain out of a bucket and she was kind of speechless as she looked at it. Finally she says "It doesn't look like spaghetti at all!" Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Thursday, March 11, 2010 8:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Stories I was introduced the color world of Histology, when I was about 7 or 8. I saw my first leg being grossed. I was the cool kid in elementary school that during show and tell, would bring in a section of brain or perhaps an embryo floating in formalin. I worked my summers filing blocks and slides (Not to worry I understood the importance of numerical order!), and as I got older would work my summers as a lab aide. After high school, and very undecided in which direction my life should go, the Histology Supervisor had encouraged as she did all her lab aides and others she felt needed to add their mark in this profession into this career. She had a histology program (at the time when it was OJT) and she would have 3 students at a time. We would work nights assisting with gross, and mornings in class. She would give us weekly exams and instill in us the importance of the profession. The majority of her students that she had taught have moved on to become supervisors and charge techs. I have to say that I come from a "family" of histologists. I was very fortunate that this woman who had an interest in my future not just in me as a person but as her daughter. You see, this supervisor was my mother, and I will forever be grateful to her for introducing me to this field. Her name is Sofia Roberts and I'm sure that there are many members that know her. So to her I say "Happy Histologist Professional Day"! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr Brandon Fl 33511 (813) 571-6410 or ext 2454 (813) 571-5169 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> umn.edu Thu Mar 11 11:19:48 2010 From: ander093 <@t> umn.edu (LuAnn Anderson) Date: Thu Mar 11 11:19:47 2010 Subject: [Histonet] histology stories Message-ID: Another funny story: When my son was in kindergarten, the teacher went around the class asking the kids what their parents did. When she got to Michael he stood up and announced quite boldly "she cuts up brains!!". The teacher asked me at the next conference what I really did and after explaining histology to her, she told me the story and said that she was afraid to ask him for more details on that day! I got a chuckle out of that and so did she! LuAnn From Bonnie.Whitaker <@t> osumc.edu Thu Mar 11 11:32:34 2010 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Thu Mar 11 11:32:42 2010 Subject: [Histonet] RE: Histology Stories In-Reply-To: References: <938D716CD445614ABBB817517557B6F4E30EBB84@NADCWPMSGCMS09.hca.corpad.net> Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F600166B042@msxc06.OSUMC.EDU> That triggered a memory for me: When my daughter was 4 or 5, she came home from day care all mad because she had gotten in trouble. It seems that the teacher had talked to the class about smoking and what it does to your lungs, and apparently described lungs somewhat inaccurately. My daughter had called her on her inaccuracy, and informed the teacher that she "had been to autopsies before, and knew what diseased lungs looked like", and basically told the teacher that she didn't know what she was talking about. When I took call for weekend autopsies, a couple of times my daughter went with me because I had nowhere leave her on Sundays if her dad was out of town, so she was certainly correct. She just lacked tact. One of our pathologists at the time, told me that she always put her son in a highchair in the morgue when she was a resident, and on autopsy duty. She faired better than I did, however. Her son became a physician, as well. My daughter was never interested in the medical field. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon Sent: Thursday, March 11, 2010 12:05 PM To: 'Jessica.Vacca@HCAhealthcare.com'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histology Stories Your introduction into histology brought back a very funny memory for me. My kids are in their 30's now, but when my daughter was 7 or 8 she also saw her first grossing specimen which was also a leg. She tried to talk about it in show and tell and her teacher made her stop. She didn't want to hear about it and my daughter was so excited about being able to share the information with her classmates. When my son was 14 or 15 he wanted to see a brain and we happened to have one from an autopsy case. I took it out and he got one whiff of the formalin and said "Mom, no wonder you are so weird, having to smell that stuff everyday". No one can appreciate what we do quite like our children!! Jessica, that was a really cool story about your Mom introducing you to histology. It brought a tear to my eye! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Thursday, March 11, 2010 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Stories I was introduced the color world of Histology, when I was about 7 or 8. I saw my first leg being grossed. I was the cool kid in elementary school that during show and tell, would bring in a section of brain or perhaps an embryo floating in formalin. I worked my summers filing blocks and slides (Not to worry I understood the importance of numerical order!), and as I got older would work my summers as a lab aide. After high school, and very undecided in which direction my life should go, the Histology Supervisor had encouraged as she did all her lab aides and others she felt needed to add their mark in this profession into this career. She had a histology program (at the time when it was OJT) and she would have 3 students at a time. We would work nights assisting with gross, and mornings in class. She would give us weekly exams and instill in us the importance of the profession. The majority of her students that she had taught have moved on to become supervisors and charge techs. I have to say that I come from a "family" of histologists. I was very fortunate that this woman who had an interest in my future not just in me as a person but as her daughter. You see, this supervisor was my mother, and I will forever be grateful to her for introducing me to this field. Her name is Sofia Roberts and I'm sure that there are many members that know her. So to her I say "Happy Histologist Professional Day"! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr Brandon Fl 33511 (813) 571-6410 or ext 2454 (813) 571-5169 FAX ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Thu Mar 11 11:59:50 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Mar 11 11:59:56 2010 Subject: [Histonet] Used equipment Message-ID: We're doing some renovating in our Histology department (YEAH!) and have some equipment that we no longer need. If no vendors are interested, we would consider donating them. Labconco Protector Laboratory Hood (47"Wx59"Hx31"D) Tissue Tek II cryostat Leica TP1050 processor MT920 microtome All equipment has had regular pm's. Anyone interested may contact me off-line. Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From greenjumpyone <@t> hotmail.com Thu Mar 11 12:01:44 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Thu Mar 11 12:01:48 2010 Subject: [Histonet] (no subject) Message-ID: http://www.globo.tur.br/bLGiY6ZPO0.html _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/ From MLunetta <@t> luhcares.org Thu Mar 11 12:02:06 2010 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Thu Mar 11 12:02:26 2010 Subject: [Histonet] Excelsior Vs VIP 6 Message-ID: <4B98CDAE020000A800040B0F@ns.luhcares.org> Hey Histo gang, We are looking for opinions not sales calls. So please do not pass this onto vendors and give us your opinon so we have good feed back from our peers. Thanks, Matt HT ASCP Longmont United Hospital From mbrooks <@t> incytepathology.com Thu Mar 11 12:27:14 2010 From: mbrooks <@t> incytepathology.com (Matt Brooks) Date: Thu Mar 11 12:27:19 2010 Subject: [Histonet] Histo Story Message-ID: <706224670091FE47997AEF88EFADE7CA01550B47@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello All, I had never heard the before starting as a Histotech trainee in Tulsa, OK. The funny story I wanted to share was similar to some others submitted. My oldest son, at five, was asked by his teacher, "What does your father do?" He replied, "He cuts up dead people". Much to my dismay at the next parent teacher conference I was asked by the teacher what I did for a living and my reply was "I am a Histotechnician and a Diener". She then asked, as most people do, "What is that?". I began to explain the histology part and that I did autopsies. Then she explained what my son had told her earlier. Knowing that I had not ever told my children that I performed autopsies it came as quite a shock. So I asked my wife if she had even mentioned this to the kids and the answer was of course "no". It turns out the babysitter had told them. Needless to say she no longer watches our children. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 From Montina.VanMeter <@t> pbrc.edu Thu Mar 11 12:34:36 2010 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Thu Mar 11 12:34:48 2010 Subject: [Histonet] Histo Stories In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4FE7FB862E90E448AE32388E759220E5020FCDA0@pbrcas31.pbrc.edu> My high school guidance counselor received a letter from Barbara Hrapchak, co-author of "The Theory and Practice of Histotechnology and director of the Ohio State University Hospital School of Histotechnology in 1975. I knew that I wanted to go into a scientific field that involved dissection as I was interested in the physiological/biological systems of different species. Being so young (17), I didn't appreciate how special and lucky that experience was with Barbara until years later. Our class (1976) consisted of four women from very different walks of life and that was an education for me in itself, as I grew up in a very rural community. We each had our preference of activities in the lab and mine was cutting. I have always taken pride in being able to produce a good section. Barbara was a stickler for perfection and cleanliness ("leaving the lab as if no one had been there"). I didn't appreciate it then, but now find myself with the same expectations of the people I train. Ironically, a few years later, Barbara became a graduate student in the research department that I worked in at Ohio State. After graduating from histotechnology school, I spent the first year in a clinical lab and realized I was better suited for the research environment. I spent the first ten years doing electron microscopy that eventually was combined with immunohistochemistry involving spinal cord injury research. After twenty-six years, I moved to Louisiana and have been involved with the entire process of experimental design, surgery, perfusions, IHC and IF, confocal imaging and authorship. My employers continue to encourage me in that process and provide funds for me to attend the state and national meetings every year. It is truly a win-win situation, as I pick up so many worthwhile pieces of information that can make our histology remain on the cutting edge. My boss has often said, "he'll give me enough rope to hang myself". In translation that means I can do as much as I feel comfortable with in doing the background research on procedures for the mechanism of interest, designing the protocols, writing the methods for publication and troubleshooting if necessary. Those of us in animal research have learned that troubleshooting is a major part of your day when doing immunohistochemistry. After thirty-four years in Histology I continue to learn new and exciting methods and it was all because of a letter sent from Barbara, trying to incite interest in the field of Histotechnology among high school students. Inviting high school students to our state and national meetings is a wonderful advertisement for our field. Hopefully, we can encourage a new generation to enter into this wonderful vocation. Sadly, we lost Barbara to cervical cancer in 1991 at the young age of forty-six. She was totally committed to the field of Histotechnology and I owe my career to her insistence of perfection in obtaining the optimal histological diagnosis for the patient. Regards, Tina Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, March 11, 2010 7:09 AM To: histonet Subject: [Histonet] Histo Stories Thanks to everyone that sent their Story of How I Ended Up Doing This Histology Thing! I have gotten 50 or more replies! The one thing that strikes me is how many of us went into this profession without a clue! With all the opportunities to recruit future histologists, this Histology Day idea is a good start. On the original subject, I'm planning to make one document out of all the replies and - WITH PERMISSION - attach your name to the answers. If you do NOT want your submission listed because you want to remain anonymous, you must let me know ASAP. Send to: nmhisto@comcast.net. Thanks for your stories! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> cvmc.org Thu Mar 11 12:38:11 2010 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Thu Mar 11 12:38:17 2010 Subject: [Histonet] Excelsior Vs. VIP 6 In-Reply-To: <4B98A0C6020000A800040A69@ns.luhcares.org> Message-ID: We just purchased a VIP 6 in January. It is wonderful - user friendly, many programming options and Tissue-Tek's reliability with its tissue processors make it the best processor (in my opinion) being sold today. Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From renafail <@t> bellsouth.net Thu Mar 11 12:50:24 2010 From: renafail <@t> bellsouth.net (Rena Fail) Date: Thu Mar 11 12:50:28 2010 Subject: [Histonet] Fw: Histo story Message-ID: <611236.5226.qm@web180314.mail.gq1.yahoo.com> ----- Forwarded Message ---- From: Rena Fail To: nmhisto@comcast.net Sent: Thu, March 11, 2010 1:48:12 PM Subject: Histo story A single parent at 29 with 4 children, unpredictable child support, and only a high school education, I could only get minimum wage jobs. I started looking into federally funded educational programs. the obvious, becoming a nurse, but that program?had?met its quota.?I wanted to work in the health field so the?adviser?started going through the college catalog to see what other 1-2 year programs were offered in the health field. When we read the very short but descriptive paragraph for histology?I knew ? that was it, that was what I wanted to do, I couldn't wait. So it was back to school at 31 and for 30 years I practiced the Art of Histology. From that short paragraph to a career in a profession I loved and for which I never loss?enthusiasm or wonder. ?For nearly 20 years I worked for the department head Gordon R. Hennigar who taught me to trust my instincts and always always add to my knowledge, read the old books and publications as well as the new. To do the very best work every day so that the pathologist could do his best for the patient.?I was also privileged to have?worked in the same department with Drs. Joseph McManus and Sam Spicer. Both of these researchers would not hesitate to answer a techs questions.? Though Sam Spicer usually responded with copies of articles he had written and a mini lecture. The last years before my retirement? my immediate boss was Vinnie who taught me to put pen to paper to share with others. i learned to write manuals,??develop stain procedures,? play with dyes, put out fires, help pathologists, work with residents and help patients, all because of one little paragraph in a college catalog. Rena Fail Retired from Medical University o fsouth Carolina? From lucy.zong <@t> gmail.com Thu Mar 11 12:55:58 2010 From: lucy.zong <@t> gmail.com (Lucy Zong) Date: Thu Mar 11 12:56:03 2010 Subject: [Histonet] operator manual Message-ID: <8daef62e1003111055v3e695e32kf1a22b41c87bb9d3@mail.gmail.com> I am in need of an operators manual for the Leica 2025 microtome , Leica 2040 microtome and the Hacker 3660 Glass coverslipper . If anyone has any of these could you please sent it to me as pdf file, I would greatly appreciate it. From lblazek <@t> digestivespecialists.com Thu Mar 11 12:54:16 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 11 12:56:36 2010 Subject: [Histonet] RE: Histo Story In-Reply-To: <706224670091FE47997AEF88EFADE7CA01550B47@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> References: <706224670091FE47997AEF88EFADE7CA01550B47@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Message-ID: <5A2BD13465E061429D6455C8D6B40E390E9BF76770@IBMB7Exchange.digestivespecialists.com> That's a funny one. My son told his teacher and classroom that his mom "cut up dead people and made slides out of them". It was a long time living that one down. He understood exactly what he meant but the rest of his class could only relate to the equipment on the playground! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Brooks Sent: Thursday, March 11, 2010 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histo Story Hello All, I had never heard the before starting as a Histotech trainee in Tulsa, OK. The funny story I wanted to share was similar to some others submitted. My oldest son, at five, was asked by his teacher, "What does your father do?" He replied, "He cuts up dead people". Much to my dismay at the next parent teacher conference I was asked by the teacher what I did for a living and my reply was "I am a Histotechnician and a Diener". She then asked, as most people do, "What is that?". I began to explain the histology part and that I did autopsies. Then she explained what my son had told her earlier. Knowing that I had not ever told my children that I performed autopsies it came as quite a shock. So I asked my wife if she had even mentioned this to the kids and the answer was of course "no". It turns out the babysitter had told them. Needless to say she no longer watches our children. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mabosso <@t> unipathllc.com Thu Mar 11 13:05:17 2010 From: mabosso <@t> unipathllc.com (Mary Abosso) Date: Thu Mar 11 13:04:03 2010 Subject: [Histonet] One more story to add Message-ID: <43A451981FF6634795BE83B1B5494D631BE0DB@exchange.unipathllc.corp> Back in 1974, I wanted to take some summer classes through my high school. There was an interesting class through the Ingham Intermediate School District called "Exploratory Health". It highlighted three vocational programs at the Career Center in Mason, Michigan, one of which was called "Histology". I spent a week being exposed to Dan Spencer, then instructor showing us what the program and Histology was all about. I was hooked and at the tender age of 14, started the process of being admitted to the program. I started in the fall of 1976 in this ASCP accredited program under the new teacher Betsy Krummery. Betsy was not a whole lot older than we were, but she was a great teacher. I went half day to the program for two years, concluding with three internships at two area hospitals and one in the Department of Human Medicine's Pathology Department at MSU. There I met one of the most influential Histotechnologists that I have ever worked with - Nina Miller. By graduation in 1978 I was board eligible, and applied at the next available time which was August. As us old timers recall, you could only apply in February and August, and then took the practical, followed by the written which I passed the 1st time and have been a registered tech for the last 31 years. Mary (Quandt) Abosso Aurora, Colorado From sbreeden <@t> nmda.nmsu.edu Thu Mar 11 13:04:56 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Mar 11 13:05:00 2010 Subject: [Histonet] Histology Stories, Part 3 Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46F52@nmdamailsvr.nmda.ad.nmsu.edu> I am stunned and amazed at the amount of response this little query has gotten! I have forwarded those that were posted here to my home email (and I've gotten many there, too) and expect that I'll get many, many more. I know not everyone has time to respond to email every single day, so I'm giving it more time. Unless you specifically request to remain anonymous, I'm going to give each contributor "credit" for their story. And because we have so many members from around the world, I would very much like to know how our fellow techs got started in histology in their countries. But this whole endeavor has been very interesting and I thank each of you for sharing your story. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From lblazek <@t> digestivespecialists.com Thu Mar 11 13:04:21 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 11 13:06:43 2010 Subject: [Histonet] RE: Histology Stories, Part 3 In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F52@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46F52@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E390E9BF76772@IBMB7Exchange.digestivespecialists.com> You have book in the making! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, March 11, 2010 2:05 PM To: histonet Subject: [Histonet] Histology Stories, Part 3 I am stunned and amazed at the amount of response this little query has gotten! I have forwarded those that were posted here to my home email (and I've gotten many there, too) and expect that I'll get many, many more. I know not everyone has time to respond to email every single day, so I'm giving it more time. Unless you specifically request to remain anonymous, I'm going to give each contributor "credit" for their story. And because we have so many members from around the world, I would very much like to know how our fellow techs got started in histology in their countries. But this whole endeavor has been very interesting and I thank each of you for sharing your story. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Thu Mar 11 13:15:02 2010 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Mar 11 13:16:27 2010 Subject: [Histonet] television coverage of Histotechnology Professionals Day In-Reply-To: <43A451981FF6634795BE83B1B5494D631BE0DB@exchange.unipathllc.corp> References: <43A451981FF6634795BE83B1B5494D631BE0DB@exchange.unipathllc.corp> Message-ID: I thought some of you might be interested to learn that our histo lab staff were interviewed yesterday by the Charleston CBS affiliate station Live 5 News. The interview was telecast last evening at about 5:30 pm so we succeeded in getting histology on the air waves. The news reporter showed up with almost no warning while I was enroute to the state capital with Wanda Smith to meet with our governor. I'm thrilled that our techs were in the spotlight. They and all of you, deserve to be recognized for all that you do to enhance our quality of life. If you'd like to see the video clip, go to http://www.live5news.com/Global/story.asp?S=12118805 on that page, off to the right, you will see the phrase "Lab techs recognized for work behind the scenes" Click on that phrase and the video will open. Turn up your speakers as the volume is a bit inconsistent. The telecast was perfect in every way. The video clip does not include two "teasers" that preceded the actual segment, you know the ones where they say "when we return, histotechs at MUSC..." Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 From mturner <@t> carisdx.com Thu Mar 11 13:25:06 2010 From: mturner <@t> carisdx.com (Turner, Mark) Date: Thu Mar 11 13:25:10 2010 Subject: [Histonet] Histo Stories In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: I began working in a lab in Northern Kentucky right out of high school as a Diener, washing glassware and assisting with autopsies. During the slow times I would hang out in Histology, watching the one tech in the building work her magic. After a year or so the lab director decided to add another tech and they agreed to train me. After a few years of OJT I sat for the exam and passed it with flying colors. I continued my education, eventually getting a PhD, but I am so glad I stumbled into the Histology field. I, too, had no idea what Histotechnology was when I was in high school, but in the edited words of Garret Morris "Histology been berry, berry good to me!" Mark Turner, HT (ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Thursday, March 11, 2010 7:59 AM To: Breeden, Sara Cc: histonet Subject: Re: [Histonet] Histo Stories Sally, I didn't get a chance to answer yesterday - I went to a high school and spoke about my favorite topic - histotechnology! Anyway, I started out in microbiology and then worked in a doctor's office lab doing routine chemistry and hematology and when we moved to a small town in Iowa the only position in the lab that became available was in histology. I was playing bridge with the histotech and he mentioned the opening so I went in and interviewed, got the job and never have looked back. So I sort of fell into this profession as did many others. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Mar 11, 2010, at 6:09 AM, Breeden, Sara wrote: > Thanks to everyone that sent their Story of How I Ended Up Doing This > Histology Thing! I have gotten 50 or more replies! The one thing > that > strikes me is how many of us went into this profession without a clue! > With all the opportunities to recruit future histologists, this > Histology Day idea is a good start. On the original subject, I'm > planning to make one document out of all the replies and - WITH > PERMISSION - attach your name to the answers. If you do NOT want your > submission listed because you want to remain anonymous, you must let > me > know ASAP. Send to: nmhisto@comcast.net. Thanks for your stories! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aobrien88 <@t> comcast.net Thu Mar 11 13:25:14 2010 From: aobrien88 <@t> comcast.net (aobrien88@comcast.net) Date: Thu Mar 11 13:25:18 2010 Subject: [Histonet] IHC worksheet reports Message-ID: <440003497.12482971268335514991.JavaMail.root@sz0008a.emeryville.ca.mail.comcast.net> I was wondering if anyone could give me some information on the IHC worksheet reports.? These are the reports that are printed off after you have completed your IHC stain.? We are using a Dako Autostainer + and an IntelliPath, we give our?Pathologist the reports with the slides?to sign off?on the?stain.? Does anyone know how long you need to keep these worksheet reports??? Thank you, Andrea O'Brien HT (ASCP)??? From scampbell <@t> celligent.net Thu Mar 11 13:30:40 2010 From: scampbell <@t> celligent.net (Campbell, Sharon) Date: Thu Mar 11 13:29:01 2010 Subject: [Histonet] histo stories Message-ID: I started out as a Med. Tech student. I changed my mind when I was working as a Phlebotomist in a Wisconsin Hospital. In the lab was a small room with 2 techs in it. Through the window I could see tissue on the counter. Curiosity won out. I soon started Histology training also at Marshfield, WI. And the rest is history. My mom often tells me that people around my home town ask her how my job in the history department is going! Sharon Campbell Sharon Campbell HT, HTL (ASCP) Histology Supervisor Celligent Diagnostics, LLC 106 Venture Blvd. Spartanburg, SC 29306 (864) 583-3850 From JWeems <@t> sjha.org Thu Mar 11 13:40:43 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Mar 11 13:40:53 2010 Subject: [Histonet] IHC worksheet reports In-Reply-To: <440003497.12482971268335514991.JavaMail.root@sz0008a.emeryville.ca.mail.comcast.net> References: <440003497.12482971268335514991.JavaMail.root@sz0008a.emeryville.ca.mail.comcast.net> Message-ID: <27648A6C5BC9B145813DF5182F83AB45075733@ITSSSXM01V1.one.ads.che.org> We keep them two years like we do other QC data. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of aobrien88@comcast.net Sent: Thursday, March 11, 2010 14:25 To: histonet Subject: [Histonet] IHC worksheet reports I was wondering if anyone could give me some information on the IHC worksheet reports.? These are the reports that are printed off after you have completed your IHC stain.? We are using a Dako Autostainer + and an IntelliPath, we give our?Pathologist the reports with the slides?to sign off?on the?stain.? Does anyone know how long you need to keep these worksheet reports??? Thank you, Andrea O'Brien HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Mar 11 13:37:00 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Mar 11 13:40:56 2010 Subject: [Histonet] IHC worksheet reports In-Reply-To: <440003497.12482971268335514991.JavaMail.root@sz0008a.emeryville.ca.mail.comcast.net> References: <440003497.12482971268335514991.JavaMail.root@sz0008a.emeryville.ca.mail.comcast.net> Message-ID: We keep ours for two years. Why I do not know. CAP says to keep the requisitions two years so we followed this rule. BUT why is a mystery - some of the pathologist mark OK on them or repeat. But most of the time they are ignored or shredded by the pathologist. So using them as QA is almost impossible, since sometimes you get them other times you don't. One of the pathologist had about 6 months of these forms in his office that he dropped off one day. How do I file these and find them ever? I am investigating this. And I will ask our NYS inspector when they come in. The CAP inspector said that we did even need them. Just the requisition was needed to track what was ordered versus what was done. The files are stored on the computer indefinitely on both the Dako and the Ventana. Basically my techs just use them to make sure that the correct doctor gets the slides. If you have any ideas please let me know. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of aobrien88@comcast.net [aobrien88@comcast.net] Sent: Thursday, March 11, 2010 2:25 PM To: histonet Subject: [Histonet] IHC worksheet reports I was wondering if anyone could give me some information on the IHC worksheet reports. These are the reports that are printed off after you have completed your IHC stain. We are using a Dako Autostainer + and an IntelliPath, we give our Pathologist the reports with the slides to sign off on the stain. Does anyone know how long you need to keep these worksheet reports? Thank you, Andrea O'Brien HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Thu Mar 11 13:42:20 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Thu Mar 11 13:42:30 2010 Subject: [Histonet] RE: television coverage of Histotechnology Professionals Day In-Reply-To: References: <43A451981FF6634795BE83B1B5494D631BE0DB@exchange.unipathllc.corp> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C49F9A@EXCHMBC2.ad.ah.local> Vinnie, Thanks for everything you have done to make the first Histotechnology Professionals Day happen. What a success on many fronts! Jan Mahoney Omaha NE Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From JWeems <@t> sjha.org Thu Mar 11 13:43:34 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Mar 11 13:43:43 2010 Subject: [Histonet] IHC worksheet reports In-Reply-To: References: <440003497.12482971268335514991.JavaMail.root@sz0008a.emeryville.ca.mail.comcast.net> Message-ID: <27648A6C5BC9B145813DF5182F83AB45075734@ITSSSXM01V1.one.ads.che.org> We use them as QC and our pathologists make notes or confirm ok is why we keep them 2 yrs as CAP dictates. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, March 11, 2010 14:37 To: aobrien88@comcast.net; histonet Subject: RE: [Histonet] IHC worksheet reports We keep ours for two years. Why I do not know. CAP says to keep the requisitions two years so we followed this rule. BUT why is a mystery - some of the pathologist mark OK on them or repeat. But most of the time they are ignored or shredded by the pathologist. So using them as QA is almost impossible, since sometimes you get them other times you don't. One of the pathologist had about 6 months of these forms in his office that he dropped off one day. How do I file these and find them ever? I am investigating this. And I will ask our NYS inspector when they come in. The CAP inspector said that we did even need them. Just the requisition was needed to track what was ordered versus what was done. The files are stored on the computer indefinitely on both the Dako and the Ventana. Basically my techs just use them to make sure that the correct doctor gets the slides. If you have any ideas please let me know. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of aobrien88@comcast.net [aobrien88@comcast.net] Sent: Thursday, March 11, 2010 2:25 PM To: histonet Subject: [Histonet] IHC worksheet reports I was wondering if anyone could give me some information on the IHC worksheet reports. These are the reports that are printed off after you have completed your IHC stain. We are using a Dako Autostainer + and an IntelliPath, we give our Pathologist the reports with the slides to sign off on the stain. Does anyone know how long you need to keep these worksheet reports? Thank you, Andrea O'Brien HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From JWeems <@t> sjha.org Thu Mar 11 14:09:32 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Mar 11 14:09:41 2010 Subject: [Histonet] television coverage of Histotechnology Professionals Day In-Reply-To: References: <43A451981FF6634795BE83B1B5494D631BE0DB@exchange.unipathllc.corp> Message-ID: <27648A6C5BC9B145813DF5182F83AB45075740@ITSSSXM01V1.one.ads.che.org> That is really exciting, Vinnie! Thanks for all you do, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Thursday, March 11, 2010 14:15 To: Histonet Subject: [Histonet] television coverage of Histotechnology Professionals Day I thought some of you might be interested to learn that our histo lab staff were interviewed yesterday by the Charleston CBS affiliate station Live 5 News. The interview was telecast last evening at about 5:30 pm so we succeeded in getting histology on the air waves. The news reporter showed up with almost no warning while I was enroute to the state capital with Wanda Smith to meet with our governor. I'm thrilled that our techs were in the spotlight. They and all of you, deserve to be recognized for all that you do to enhance our quality of life. If you'd like to see the video clip, go to http://www.live5news.com/Global/story.asp?S=12118805 on that page, off to the right, you will see the phrase "Lab techs recognized for work behind the scenes" Click on that phrase and the video will open. Turn up your speakers as the volume is a bit inconsistent. The telecast was perfect in every way. The video clip does not include two "teasers" that preceded the actual segment, you know the ones where they say "when we return, histotechs at MUSC..." Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From cpyse <@t> x-celllab.com Thu Mar 11 14:43:42 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Thu Mar 11 14:44:17 2010 Subject: [Histonet] Excelsior Vs. VIP 6 In-Reply-To: <4B98A0C6020000A800040A69@ns.luhcares.org> References: <4B98A0C6020000A800040A69@ns.luhcares.org> Message-ID: <003001cac15b$898bddb0$9ca39910$@com> Matt We have both the VIP 6(new)and the Excelsior(older). The Excelsior is a little less user friendly but is a workhorse if maintained properly. There is less solution exposure since you do not have to fill the containers as you do in the VIP6. Both machines produce excellent tissue blocks. The VIP6 has many safe guards so there is little chance for mistakes. With every step in the VIP6 it asks for verification of the command. If you have any more question I can answer feel free to contact me. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com 716-250-9235 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Thursday, March 11, 2010 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Excelsior Vs. VIP 6 Hello to the World Wide Histo's, We are looking at getting a new processor and was wondering what everyones feelings were on the Excelsior from Thermo vs the VIP 6 from Sakura. We have a VIP5 right now and it is a delight. The demo of the Excelsior was very impressive. Thanks for your thoughts. Matt HT (ASCP) Longmont United Hospital Longmont, Colorado _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Mar 11 15:20:03 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Mar 11 15:20:07 2010 Subject: [Histonet] Re: Formalin vs alcoholic formalin Message-ID: William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska asks: >>Help! I think I know the answer, but need some rapid clarification. Is an alcohol-formalin fixative acceptable for use in breast tissue, or does it need to be an aqueous 10% NBF?<< According to the FDA, the required fixation for the HER2 immunostain on breast tissue is neutral buffered formalin - accept no substitutes! I agree with them. Bob Richmond Samurai Pathologist Knoxville TN From marilyn <@t> adcgca.com Thu Mar 11 15:27:25 2010 From: marilyn <@t> adcgca.com (marilyn@adcgca.com) Date: Thu Mar 11 15:27:29 2010 Subject: [Histonet] Looking for Darlene G Jones Message-ID: <3e87f897558da6f91c1ec14fb6131f02@> I would like Darlene Jones to contact me. I have an article she published in the Lab Leader(Shandon-Lipshaw newsletter)in 1996. WOuld love to republish it in our state newsletter. If you know Darlene, please tell her to contact me. Thank you, Marilyn McDonald, HT(ASCP) Little Rock, AR From algranth <@t> email.arizona.edu Thu Mar 11 15:27:42 2010 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Mar 11 15:27:53 2010 Subject: [Histonet] RE: Histology Stories In-Reply-To: References: <938D716CD445614ABBB817517557B6F4E30EBB84@NADCWPMSGCMS09.hca.corpad.net> Message-ID: <9FC20CFB-6D97-4E96-AE2D-6554DB02F976@email.arizona.edu> This story reminds me of the time I had to run into the lab over a weekend and I had my son with me. He was about 6 or 7 at the time. He tripped over a bucket containing a brain suspended for fixing in formalin from an autopsy that was just done and as I was cautioning him to be more careful he asked, "what kind of surgery did that person have?" Needless to say our dinnertime conversations were very interesting. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Mar 11, 2010, at 10:05 AM, Beckham, Sharon wrote: > Your introduction into histology brought back a very funny memory > for me. My kids are in their 30's now, but when my daughter was 7 > or 8 she also saw her first grossing specimen which was also a leg. > She tried to talk about it in show and tell and her teacher made her > stop. She didn't want to hear about it and my daughter was so > excited about being able to share the information with her > classmates. When my son was 14 or 15 he wanted to see a brain and > we happened to have one from an autopsy case. I took it out and he > got one whiff of the formalin and said "Mom, no wonder you are so > weird, having to smell that stuff everyday". No one can appreciate > what we do quite like our children!! > > Jessica, that was a really cool story about your Mom introducing you > to histology. It brought a tear to my eye! > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu > ] On Behalf Of Jessica.Vacca@HCAhealthcare.com > Sent: Thursday, March 11, 2010 10:49 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Stories > > I was introduced the color world of Histology, when I was about 7 or > 8. I saw my first leg being grossed. I was the cool kid in > elementary school that during show and tell, would bring in a > section of brain or perhaps an embryo floating in formalin. I worked > my summers filing blocks and slides (Not to worry I understood the > importance of numerical order!), and as I got older would work my > summers as a lab aide. After high school, and very undecided in > which direction my life should go, the Histology Supervisor had > encouraged as she did all her lab aides and others she felt needed > to add their mark in this profession into this career. She had a > histology program (at the time when it was OJT) and she would have 3 > students at a time. We would work nights assisting with gross, and > mornings in class. She would give us weekly exams and instill in us > the importance of the profession. The majority of her students that > she had taught have moved on to become supervisors and charge techs. > I have to say that I come from a "family" of histologists. I was > very fortunate that this woman who had an interest in my future not > just in me as a person but as her daughter. You see, this supervisor > was my mother, and I will forever be grateful to her for introducing > me to this field. Her name is Sofia Roberts and I'm sure that there > are many members that know her. So to her I say "Happy Histologist > Professional Day"! > > Jessica Vacca > Histology Supervisor > Brandon Regional Hospital > 119 Oakfield Dr > Brandon Fl 33511 > (813) 571-6410 > or ext 2454 > (813) 571-5169 FAX > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Thu Mar 11 16:08:21 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Mar 11 16:08:26 2010 Subject: [Histonet] H. pylori on Rhesus macaque tissue Message-ID: <582736991003111408x1b689771m24f87bc6cd0cd65b@mail.gmail.com> Hi, I am sure this will work. Granted I haven't tried it... The antibody is directed against the bug, the H. pylori bacteria, not against a product of the human it exists in. So it shouldn't matter where the bug is, it should have the epitope the antibody binds with. I do Hepatitis B on non human tissue all the time and it works fine. Good luck, (and let me know for sure how it goes) Amos Brooks Message: 9 Date: Thu, 11 Mar 2010 11:25:16 -0500 From: "Zerfas, Patricia (NIH/OD/ORS) [E]" Subject: [Histonet] H. pylori on Rhesus macaque tissue To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear List servers, Did anyone try this antibody on Rhesus macaque tissue? Do you have a protocol? How were the results? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 From ElizabethWyand <@t> texashealth.org Thu Mar 11 16:31:16 2010 From: ElizabethWyand <@t> texashealth.org (Wyand, Elizabeth) Date: Thu Mar 11 16:31:31 2010 Subject: [Histonet] Job Openings in Plano, TX Message-ID: We currently have 2 openings for our private lab in Plano, TX: Benefits include fully paid medical, dental, life insurance, PTO time, generous retirement package. One Full Time Histotech. Must be ASCP registered HT or HTL, or eligible (must become registered within 10 months of hire date). Duties include embedding, microtomy, IHC, special stains. One Full Time Flow Cytometry Tech needed for start up operation. The individual will be responsible for the clinical flow cytometry testing, to include assay validation, implementation, and testing. The scope includes sample preparation, flow cytometric acquisition and analysis, as well as documenting quality control and coordinating quality management for the flow cytometry section. Current experience with assay optimization, validation and antibody cocktailing; current experience with flow cytometry, specifically hematological malignancies; knowledge of CAP requirements for flow cytometry testing; writing and updating laboratory policies and procedures. BS degree in a relevant science with certification in medical technology, histotechnology or cytotechnology required. Interested candidates please contact Genie Jacobs at 972-981-3108 or fax resume to 972-981-3236. The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From pathmaster <@t> yahoo.com Thu Mar 11 17:28:06 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Thu Mar 11 17:28:10 2010 Subject: [Histonet] Happy Histo Day Message-ID: <998355.3837.qm@web111110.mail.gq1.yahoo.com> No celebration here in Bay Shore. So sad. My story is a long one, some of you might find it interesting. It was 10 years ago that Vinnie encouraged me to put my story to pen and paper and suggested also that we republish my Movat pentachrome modification from the 1972 Histo Logic.? The link is here, just scroll down to Memoirs of a Self Made Histotech. (Parts one and two :-) A lot of water under the bridge since that was finished. http://www.sakura-americas.com/histologic/topics/history.html Best wished and Happy Belated Holiday to all my colleagues out there. And, if? anyone reading in Europe has any idea how I might find a situation over there, please let me know. I'm itching for something different. Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant, Histology Supervisor, Laboratory Safety Officer From kimtournear <@t> yahoo.com Thu Mar 11 18:01:14 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Mar 11 18:01:18 2010 Subject: [Histonet] recyclers Message-ID: <495257.45607.qm@web54207.mail.re2.yahoo.com> Does anyone know of any companies out there that buy used?recyclers and?refurbish them?for?re-sale? I have a xylene recycler (10 yrs old), alcohol recycler (5 yrs old) and a formalin recycler (3 yrs old) all of which?work fine. They are all CBG? products. Thanks.... ? ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! From gareth.davis <@t> hotmail.com Thu Mar 11 18:32:33 2010 From: gareth.davis <@t> hotmail.com (Gareth Blaeuer Davis) Date: Thu Mar 11 18:32:41 2010 Subject: [Histonet] Red Chromogen for MITF antibody Message-ID: Hi, Does anyone do MITF immunostains with a Red Chromogen. We are using a Dako autostainer, and in the past have used AEC chromogen on MITF. Our pathologist doesn't like it, because it doesn't get red enough. We would like something we could use with current protocols on the Dako, but can't find anything. Any suggestions? Gareth Blaeuer Davis, HT, BS. Pathology Associates of St. Thomas Nashville, Tn 37205 615-298-4100 _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/ From Robert.D.Kobus <@t> Medstar.net Thu Mar 11 19:46:13 2010 From: Robert.D.Kobus <@t> Medstar.net (Robert.D.Kobus@Medstar.net) Date: Thu Mar 11 19:46:21 2010 Subject: [Histonet] Robert D Kobus is out of the Laboratory. Message-ID: I will be out of the office starting Thu 03/11/2010 and will not return until Mon 03/15/2010. I will be out of the Laboratory from Friday 03/12/2010 until 3/15/2010. If you have a matter that can not wait, please page me at 202-801-4851. Otherwise I will return your e-mail on Monday! CONFIDENTIAL: The information contained in this communication, including its attachments may contain confidential information and is intended only for the individual (s) or entity (ies) to whom it is addressed . The information contained in this communication may also be protected by legal privilege , federal law or other applicable law. If you are not the intended recipient of this communication , you are hereby notified that any distribution, dissemination or duplication of this communication is strictly prohibited. If you have received this communication in error please immediately delete and destroy all copies of this message and please immediately notify us of the error by separate communication . Thank you. From jinhui <@t> uta.edu Fri Mar 12 00:42:36 2010 From: jinhui <@t> uta.edu (Shen, Jinhui) Date: Fri Mar 12 00:43:59 2010 Subject: [Histonet] Need help with FISH staining protocol on In-Reply-To: <52cb0f131003110336r7f617f5hbef28dc6fea87f9d@mail.gmail.com> References: <52cb0f131003110336r7f617f5hbef28dc6fea87f9d@mail.gmail.com> Message-ID: Dear Julie, Thanks for the information! I saw some papers about that staining on mice tissues, such as http://www.pnas.org/content/104/10/4030.abstract . I have been talking with Ms Leiker at Univ. of Bufflo, sorry I forgot to click "reply to all" but "reply". She gave me some good suggestions and I am going to try the staining again next week, will let you know if it works out or if I need more help. Thanks! Regards, Jinhui ________________________________________ From: J C [jcbook@gmail.com] Sent: Thursday, March 11, 2010 5:36 AM To: Shen, Jinhui Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need help with FISH staining protocol on Dear Jinhui! We tried for a long time to perform SRY FISH for rats, paraffin, frosen nothinhg worked. I know other people that did not suceeded on other animals, I do not know anybody that could do it exept of somebody in German, that worked with special plasmid for rat. There are not repeated works in pubmed. I doubt that this method works good for rats or mices, but Cumbio has a kit, maybe it will work. Regards, Julie From susanbachus <@t> verizon.net Fri Mar 12 06:44:22 2010 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Fri Mar 12 06:44:42 2010 Subject: [Histonet] Histo Stories In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <65C621FE47744D99BDA95D8A3F5B49FE@OwnerPC> Sorry to be so late to the party, hope I'm not too late to share my story: I still vividly remember being shown a "career documentary" film about histotechnology, in junior high school, back in the 60's, by my biology teacher, Lynda McCurdy Ballingall--the lovely lady who influenced my life more than anyone else, and who I just enjoyed the privilege of visiting in Chicago, where she lives now, when the Society for Neuroscience meeting was held there last year. (She swears she remembers me staying after class to continue drawing what we were observing under the microscope, though I have absolutely no recollection of this!) But I didn't think much more about it till I earned my PhD in Psychobiology, decades later. Of course in psychobiology research histology is an important method, to verify lesion damage, electrode and cannula placements, etc. Back in the "good old days" a psychobiology lab supported a technician, an animal caretaker, a secretary, and a histologist, in addition to grad students and the primary investigator. I began as that golden era was waning though, at least in our lab. I survived by teaching undergrads, and the histologist was being "let go". Just before she left, our delightful histologist, Mrs. Anne Madsen, patiently trained me, and I then trained the students who came after me. The only drawback was that she was so expert that everything worked perfectly in her hands. It then took me the next few decades to painfully learn by trial & error how to troubleshoot as things gradually went wrong (pH's drifted off, gelatin congealed, etc. etc.). I gradually added other related methods to my armamentarium, like immunohistochemistry and in situ hybridization histochemistry, while a post-doc at NIH. Not least of the "goods" that I reaped at NIH were the supplies that labs discarded when they moved, which I hoarded in my basement at home in anticipation of setting up a lab of my own in academia, where funds don't flow as freely as those at NIH, someday--enough, it turned out, to set up my own functional lab nearby at George Mason University! So when I began teaching a graduate level Histology/Histochemistry course that I kluged together on my own at GMU (thank goodness for Carson's textbook!), several years ago, I made a big deal of teaching my graduate students the nitty-gritty "nuts & bolts" theories/rationales underlying the methods, to prepare them for troubleshooting in their own futures! Some of you may recall that one of my students inadvertently stirred up a flurry of debate about the purpose of Histonet a few years ago when she asked a question that was misinterpreted as "cheating" while utilizing Histonet as a "resource" (not what I had in mind when I encouraged them to subscribe to Histonet!) in preparing for a lab that embedded a "mystery" to solve in the exercise of learning how to use Nissl stains (trying to make things more interesting). One downside to having learned "on the scene", as it were, is that I never received formal training or licensing in histology. So now that I find myself unable to afford to continue indulging in teaching as an adjunct ($3K for a 5 credit class), since my husband's death, I also find myself excluded from "real jobs" in histology. (If anyone in the DC/Northern Virginia area could use someone like me, please email me!) I laughed a lot over others' stories of their kids' exposure to their work. Mine were also practically raised in labs (my husband was also a scientist [electrophysiologist]). They have more vivid memories of the animals we used in our psychopharmacology research though--to this day (my son is graduating from college now) they proudly profess to never having felt the slightest temptation to abuse drugs (thank goodness!), having been appalled by the pathetic bedraggled appearance of our drug-injected rat subjects when they were knee-high. I did have fun taking slides in to show their junior high school biology classes, when they learned microscopy, though it seems this was more exciting for me than it was for my kids (their classmates did send warmly appreciative notes thanking me!). nostalgically, Susan ----- Original Message ----- From: "Breeden, Sara" To: "histonet" Sent: Thursday, March 11, 2010 8:09 AM Subject: [Histonet] Histo Stories > Thanks to everyone that sent their Story of How I Ended Up Doing This > Histology Thing! I have gotten 50 or more replies! The one thing that > strikes me is how many of us went into this profession without a clue! > With all the opportunities to recruit future histologists, this > Histology Day idea is a good start. On the original subject, I'm > planning to make one document out of all the replies and - WITH > PERMISSION - attach your name to the answers. If you do NOT want your > submission listed because you want to remain anonymous, you must let me > know ASAP. Send to: nmhisto@comcast.net. Thanks for your stories! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.436 / Virus Database: 271.1.1/2735 - Release Date: 03/10/10 19:33:00 -------------- next part -------------- No virus found in this outgoing message. Checked by AVG - www.avg.com Version: 8.5.436 / Virus Database: 271.1.1/2739 - Release Date: 03/11/10 21:50:00 From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Mar 12 07:23:28 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Mar 12 07:24:56 2010 Subject: [Histonet] Red Chromogen for MITF antibody In-Reply-To: References: Message-ID: We use Diagnostic Biosystems Perma Red with the Dako system. www.dbiosys.com Works very well Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gareth Blaeuer Davis [gareth.davis@hotmail.com] Sent: Thursday, March 11, 2010 7:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Red Chromogen for MITF antibody Hi, Does anyone do MITF immunostains with a Red Chromogen. We are using a Dako autostainer, and in the past have used AEC chromogen on MITF. Our pathologist doesn't like it, because it doesn't get red enough. We would like something we could use with current protocols on the Dako, but can't find anything. Any suggestions? Gareth Blaeuer Davis, HT, BS. Pathology Associates of St. Thomas Nashville, Tn 37205 615-298-4100 _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Fri Mar 12 08:30:40 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Mar 12 08:30:48 2010 Subject: [Histonet] alcian blue van gieson stain Message-ID: We want to try the double stain alcian blue Van gieson. Do you stain for the alcian blue first and then the Van gieson? We do both stains but I am unsure of how to put them together. Margaret Perry South Dakota State University From annigyg <@t> gmail.com Fri Mar 12 09:41:57 2010 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Fri Mar 12 09:42:03 2010 Subject: [Histonet] Block patient IDs Message-ID: A question for all you CAP fundis: how many patient Identifiers are needed on each paraffin block We currently just write the unique, LIS generated number on the block face, but have recently been advised that CAP and JCIA require not one, but TWO patient IDS on the block Comments please -- Anne van Binsbergen (Hope) Abu Dhabi UAE From flnails <@t> texaschildrens.org Fri Mar 12 09:54:59 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Mar 12 09:55:19 2010 Subject: [Histonet] Block patient IDs In-Reply-To: References: Message-ID: It is my understand that the two patient identifier applies to the completed slide not the block. You can verify this by looking at the CAP Checklist for anatomical pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Friday, March 12, 2010 9:42 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Block patient IDs A question for all you CAP fundis: how many patient Identifiers are needed on each paraffin block We currently just write the unique, LIS generated number on the block face, but have recently been advised that CAP and JCIA require not one, but TWO patient IDS on the block Comments please -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From Terri.Brown <@t> Northside.com Fri Mar 12 09:59:44 2010 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Fri Mar 12 09:59:53 2010 Subject: [Histonet] Block patient IDs References: Message-ID: <731941C266951A47BEF11E5EFAAED9C90196E0DF@nsmvexch01.northside.local> I think you will find that CAP is asking if 2 patient identifiers are used throughout the entire process. We engrave the blocks, slides and a computer generated block count log that uses 2 patient identifiers. Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, March 12, 2010 10:55 AM To: 'Anne van Binsbergen'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs It is my understand that the two patient identifier applies to the completed slide not the block. You can verify this by looking at the CAP Checklist for anatomical pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Friday, March 12, 2010 9:42 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Block patient IDs A question for all you CAP fundis: how many patient Identifiers are needed on each paraffin block We currently just write the unique, LIS generated number on the block face, but have recently been advised that CAP and JCIA require not one, but TWO patient IDS on the block Comments please -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From kimtournear <@t> yahoo.com Fri Mar 12 10:05:09 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Mar 12 10:05:14 2010 Subject: [Histonet] recyclers In-Reply-To: References: <495257.45607.qm@web54207.mail.re2.yahoo.com> Message-ID: <333644.31455.qm@web54206.mail.re2.yahoo.com> Looks like I'll be contacting IMEB...Thanks everyone for all?your help... ? ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! ________________________________ From: "Howery, Jeffrey" To: Kim Tournear Sent: Fri, March 12, 2010 9:01:28 AM Subject: RE: [Histonet] recyclers Try IMEB they are in Cali. Contact Dawn Yepez @ 1-800-543-8496 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kim Tournear Sent: Thu 3/11/2010 5:01 PM To: Histonet Subject: [Histonet] recyclers Does anyone know of any companies out there that buy used?recyclers and?refurbish them?for?re-sale? I have a xylene recycler (10 yrs old), alcohol recycler (5 yrs old) and a formalin recycler (3 yrs old) all of which?work fine. They are all CBG? products. Thanks.... ? ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! ????? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Bonnie.Whitaker <@t> osumc.edu Fri Mar 12 10:34:39 2010 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Fri Mar 12 10:34:52 2010 Subject: [Histonet] Block patient IDs In-Reply-To: <731941C266951A47BEF11E5EFAAED9C90196E0DF@nsmvexch01.northside.local> References: <731941C266951A47BEF11E5EFAAED9C90196E0DF@nsmvexch01.northside.local> Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F600166B04B@msxc06.OSUMC.EDU> Hi Everyone, Maybe there is something new, but we had our inspection last week, and the customized checklist used for our inspection does not require 2 identifiers. It states: NOTE: An unambiguous system of specimen identification coupled with a legible, sequential cassette and slide labeling system that withstands reagents and stains are essential to fulfill this requirement. The number of blocks processed and the number of slides prepared for each case must be recorded. ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. ANP.21150 Phase II N/A YES NO Are slides identified permanently with adequate, legible information? NOTE: Each slide must be identified by the entire accession number and descriptive letters unique to the block from which it is cut. Other appropriate identifiers should be included as applicable (e.g., levels of sectioning). Automated prelabeling systems are acceptable. Regardless of whether the identifying information is on the slide or on a label, the information must be indelible, legible and able to withstand all stages of processing and conditions of storage. The laboratory director is responsible for ensuring that slides are adequately, permanently identified. I don't know if that helps, but these were copied and pasted directly from the checklist. Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Brown Sent: Friday, March 12, 2010 11:00 AM To: Nails, Felton; Anne van Binsbergen; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs I think you will find that CAP is asking if 2 patient identifiers are used throughout the entire process. We engrave the blocks, slides and a computer generated block count log that uses 2 patient identifiers. Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, March 12, 2010 10:55 AM To: 'Anne van Binsbergen'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs It is my understand that the two patient identifier applies to the completed slide not the block. You can verify this by looking at the CAP Checklist for anatomical pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Friday, March 12, 2010 9:42 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Block patient IDs A question for all you CAP fundis: how many patient Identifiers are needed on each paraffin block We currently just write the unique, LIS generated number on the block face, but have recently been advised that CAP and JCIA require not one, but TWO patient IDS on the block Comments please -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Fri Mar 12 10:47:24 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Mar 12 10:48:10 2010 Subject: [Histonet] Block patient IDs In-Reply-To: <3CE20ED86C4A114EBDF3BCE8DEFD8F600166B04B@msxc06.OSUMC.EDU> References: <731941C266951A47BEF11E5EFAAED9C90196E0DF@nsmvexch01.northside.local> <3CE20ED86C4A114EBDF3BCE8DEFD8F600166B04B@msxc06.OSUMC.EDU> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB22669E4FA@chi2k3ms01.columbuschildrens.net> I believe you are correct Bonnie. My understanding is that the 2 patient identifier regulation came originally from JCAHO and only refers to the specimen identification as it comes to the lab, and during accessioning, but not during subsequent processing, staining etc. Mind you, I'm willing to put money on it that it is just a matter of time before that changes. I would imagine once a few more companies perfect their specimen tracking systems that CAP will mandate two patient identifiers throughout the process of specimen reception to filing. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Whitaker, Bonnie Sent: Friday, March 12, 2010 11:35 AM To: Terri Brown; Nails, Felton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs Hi Everyone, Maybe there is something new, but we had our inspection last week, and the customized checklist used for our inspection does not require 2 identifiers. It states: NOTE: An unambiguous system of specimen identification coupled with a legible, sequential cassette and slide labeling system that withstands reagents and stains are essential to fulfill this requirement. The number of blocks processed and the number of slides prepared for each case must be recorded. ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. ANP.21150 Phase II N/A YES NO Are slides identified permanently with adequate, legible information? NOTE: Each slide must be identified by the entire accession number and descriptive letters unique to the block from which it is cut. Other appropriate identifiers should be included as applicable (e.g., levels of sectioning). Automated prelabeling systems are acceptable. Regardless of whether the identifying information is on the slide or on a label, the information must be indelible, legible and able to withstand all stages of processing and conditions of storage. The laboratory director is responsible for ensuring that slides are adequately, permanently identified. I don't know if that helps, but these were copied and pasted directly from the checklist. Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Brown Sent: Friday, March 12, 2010 11:00 AM To: Nails, Felton; Anne van Binsbergen; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs I think you will find that CAP is asking if 2 patient identifiers are used throughout the entire process. We engrave the blocks, slides and a computer generated block count log that uses 2 patient identifiers. Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, March 12, 2010 10:55 AM To: 'Anne van Binsbergen'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs It is my understand that the two patient identifier applies to the completed slide not the block. You can verify this by looking at the CAP Checklist for anatomical pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Friday, March 12, 2010 9:42 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Block patient IDs A question for all you CAP fundis: how many patient Identifiers are needed on each paraffin block We currently just write the unique, LIS generated number on the block face, but have recently been advised that CAP and JCIA require not one, but TWO patient IDS on the block Comments please -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Ronald.Houston <@t> nationwidechildrens.org Fri Mar 12 10:59:07 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Mar 12 10:59:14 2010 Subject: [Histonet] Pediatric Pathology Labs Message-ID: <979FF5962E234F45B06CF0DB7C1AABB22669E4FB@chi2k3ms01.columbuschildrens.net> Would be obliged if the managers/supervisors of any pathology lab in a Children's facility please contact me off-line? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From renafail <@t> bellsouth.net Fri Mar 12 11:06:33 2010 From: renafail <@t> bellsouth.net (Rena Fail) Date: Fri Mar 12 11:06:37 2010 Subject: [Histonet] alcian blue van gieson stain In-Reply-To: References: Message-ID: <732300.1742.qm@web180313.mail.gq1.yahoo.com> Alcian Blue first ----- Original Message ---- From: "Perry, Margaret" To: "histonet@lists.utsouthwestern.edu" Sent: Fri, March 12, 2010 9:30:40 AM Subject: [Histonet] alcian blue van gieson stain We want to try the double stain alcian blue Van gieson.? Do you stain for the alcian blue first and then the Van gieson?? We do both stains but I am unsure of how to put them together. Margaret Perry South Dakota State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hymclab.hymclab <@t> ministryhealth.org Fri Mar 12 11:08:23 2010 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Fri Mar 12 11:08:31 2010 Subject: [Histonet] Block patient IDs In-Reply-To: References: Message-ID: It refers to the original container the specimen came to you in. The block is considered a secondary container, therefore, two identifiers are not required. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, March 12, 2010 9:55 AM To: 'Anne van Binsbergen'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs It is my understand that the two patient identifier applies to the completed slide not the block. You can verify this by looking at the CAP Checklist for anatomical pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Friday, March 12, 2010 9:42 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Block patient IDs A question for all you CAP fundis: how many patient Identifiers are needed on each paraffin block We currently just write the unique, LIS generated number on the block face, but have recently been advised that CAP and JCIA require not one, but TWO patient IDS on the block Comments please -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From Timothy.Morken <@t> ucsfmedctr.org Fri Mar 12 11:16:31 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Mar 12 11:16:38 2010 Subject: [Histonet] Artisan and time savings Message-ID: <1AAF670737F193429070841C6B2ADD4C013AAF77E0@EXMBMCB15.ucsfmedicalcenter.org> Hi all, For any lab using the Dako Artisan for special stains, have you documented time savings using the Artisan over manual methods? Any info you have will be helpful Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA From tjay30 <@t> yahoo.com Fri Mar 12 11:55:54 2010 From: tjay30 <@t> yahoo.com (Timothy Jay) Date: Fri Mar 12 11:55:58 2010 Subject: [Histonet] P/T Job Opening in Santa Rosa, CA Message-ID: <756646.89264.qm@web34307.mail.mud.yahoo.com> New lab in Santa Rosa looking for a P/T licensed histotech. Submit questions, resume, and references to Timothy Garcia-Jay at tjay30@yahoo.com. Thank you. ? Tim From ryaskovich <@t> dir.nidcr.nih.gov Fri Mar 12 12:56:33 2010 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Fri Mar 12 12:56:46 2010 Subject: [Histonet] Rack for round cover slips Message-ID: Does anyone have a vendor that has racks that will hold up to 30 to 50 25-mm round coverslips? Vendors welcome. Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainiofacial Research Neurobiology and Pain Therapeutics From JEllin <@t> yumaregional.org Fri Mar 12 13:12:08 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Mar 12 13:12:22 2010 Subject: [Histonet] Block patient IDs References: Message-ID: <29BE166A2CF48D459853F8EC57CD37E801611469@EXCHANGECLUSTER.yumaregional.local> In the latest CAP inspection list that we were handed, block and slides are said to have two perminant identifiers. There are alot of new items in this checklist that raise questions. This raises the question if there are alot of labs out of compliance? There are also new questions that pertain to fna samples at the bedside, etc. Also those doing digital imaging there are about 15 new questions from calibration to personal able to do the testing. There are new revisions on how we should be validating antibodies. What we are seeing is a paradigm shift in quality management. This is the biggest thing that I see, but what we are not seeing the resources that need to be deployed for this type of shift. Any takers on this. I acctually gave a presentation on this for the Arizona Society of Histology last November in Tucson. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center 928-336-1144 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of hymclab Sent: Fri 3/12/2010 10:08 AM To: 'Nails, Felton'; 'Anne van Binsbergen'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs It refers to the original container the specimen came to you in. The block is considered a secondary container, therefore, two identifiers are not required. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://connect.yumaregional.org/CitrixFEI/composemessage.asp?to=histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, March 12, 2010 9:55 AM To: 'Anne van Binsbergen'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs It is my understand that the two patient identifier applies to the completed slide not the block. You can verify this by looking at the CAP Checklist for anatomical pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://connect.yumaregional.org/CitrixFEI/composemessage.asp?to=histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Friday, March 12, 2010 9:42 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Block patient IDs A question for all you CAP fundis: how many patient Identifiers are needed on each paraffin block We currently just write the unique, LIS generated number on the block face, but have recently been advised that CAP and JCIA require not one, but TWO patient IDS on the block Comments please -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From zodiac29 <@t> comcast.net Fri Mar 12 13:27:07 2010 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Fri Mar 12 13:27:10 2010 Subject: [Histonet] HT exam Message-ID: <767812279.1174981268422027247.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Hello to all, I am in the process of trying to study for the HT exam. I was wondering if anyone has any study aids they would be willing to share? I would greatly appreciate it. Right now I have F. Carson's book that I am reviewing. There is so much information to study, I'm not sure how to approach it. Any advice would be a great help. Thanks, Jenny From Herrick.James <@t> mayo.edu Fri Mar 12 14:18:41 2010 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Fri Mar 12 14:18:47 2010 Subject: [Histonet] Processing Question Message-ID: <7267A64D75F58241B577876D8A885631015A99FA@msgebe41> Dear Histonet Colleagues, I am trying to develop a processing protocol for our Leica TP1020, but the first run has not been very successful. The specimen is canine femur (section lengths of 1" to 1-1/2 "). Following is a list of the processing reagents and times for my first attempt: 70% ETOH for 4 hours with vacuum 95% ETOH for 4 hours with vacuum 95% ETOH for 4 hours with vacuum 95% ETOH for 4 hours with vacuum 100% ETOH for 8 hours with vacuum 100% ETOH for 8 hours with vacuum 100% ETOH for 8 hours with vacuum 100% ETOH for 8 hours with vacuum Methyl Methacrylate (Pure) for 4 hours with vacuum Methyl Methacrylate (Pure) for 6 hours with vacuum Methyl Methacrylate (Pure) for 8 hours with vacuum Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum (specimen was placed into refrigerator overnight) Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum (specimen was placed into refrigerator overnight) Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum (specimen was placed into refrigerator overnight) Specimen was removed from refrigerator and embedded in a water bath for 2 days. I cut the specimen in half in the longitudinal direction. The cortices appear to have been infiltrated fairly well, but the marrow still has some very large holes. If anyone would happen to have a protocol that works well with this type of tissue and this processor, it would be greatly appreciated if you could offer your advice and expertise as to what I should do next. I do have one more specimen still running in the processor and will probably embed it on Monday. The first specimen was started on a Monday and embedded the following Monday (approx. 8 days), so this second specimen will have been in MMA for an extra 8 days before embedding. Thank you very much for your help. Jim From kdwyer3322 <@t> aol.com Fri Mar 12 16:13:29 2010 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Fri Mar 12 16:13:42 2010 Subject: [Histonet] Texas Society For Histotechnology Meeting April 22-25, 2010 - Houston Texas Message-ID: <8CC9048616B6EEB-4700-5CAE@webmail-m055.sysops.aol.com> Dear Histotnetters: It is not to late to register for the TSH meeting in April. Choose from 16 workshops and symposiums. Go to txsh.org for the complete program. It is not to late to sign up for the TSH golf outing on Thursday April 22, 2010 at 1:00 in Houston, TX. The golf outing is open to all vendors, techs and those who just want to play golf! Food, Fun and Prizes! To register please contact Kathy Dwyer at kdwyer3322@aol.com We hope to see you in TEXAS! TSH Convention Committee = From pathmaster <@t> yahoo.com Fri Mar 12 16:38:54 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Fri Mar 12 16:38:58 2010 Subject: [Histonet] (no subject) Message-ID: <966136.78483.qm@web111105.mail.gq1.yahoo.com> Hi Tim, I've been running an Artisan for 10 years both for tinctorial (or "old man" stains as one sassy younger pathologist I know calls them) and IHC stains. This thing is the best.? Never counted the minutes, but I sure am glad I had one today for all my trichromes, AFB and GMS, as well as 9 sentinel nodes and their negative controls and a skin tumor panel of cytok, CD34, actin, S-100, Mart 1. All I know is it took me 15 minutes to set up the unit, prime the reagents and load the slides,? the run took 3 hours 25 minutes, and it took me another 5-10 minutes to remove the slides and run them down to coverslip and shut down the machine. The other thing is the reliability and uniformity of the stains- who wants to check and? differentiate hot GMS slides?? I am crying though that Dako will cease to support IHC on the Artisan platform in the next 18 months or so. The stains are absolutely beautiful. Let's all beg and grovel, shall we? Good weekend to all- we're looking at up to 4 inches of rain here on Lawn Guyland Jeff Silverman- Artisan jockey From vrodriguez10 <@t> gmail.com Sat Mar 13 09:35:15 2010 From: vrodriguez10 <@t> gmail.com (Valerie Rodriguez) Date: Sat Mar 13 09:35:43 2010 Subject: [Histonet] Hello everyone, I am new to this forum Message-ID: Hello everyone, I want to introduce myself to all the histotechs here. I have visited this forum before but I finally subscribed today. My name is Valerie, but please call me Val. I am an histotechnologist living in Puerto Rico. I got interested in the field of histology in 2008. I always loved Biology but I really did not wanted to study medicine, so I started to search for careers that were related to medicine but did not required a doctorate degree. First I wanted to study cytotechnology in my country but I did not got admitted to that college because there were many people who applied to the program but a very small group was chosen. I remember that my dermatologist told me that I should consider the career of histotechnology instead because they are not a lot of people prepared in this field. Since there are no histotech schools in Puerto Rico I decided to study this career in the U.S. I graduated in 2009. I took the HTL ASCP test in January of 2010 and successfully passed after my second try. I finally got a job, which I started this month in a private laboratory in my country, but instead of working with histology, I am preparing cytology slides! At the begining I was told I was going to do histology in their central laboratory, but I was sent to another laboratory in a private hospital to perform frozen sections but to my surpise, they also told me I was going to stain cytology stains of fine needle aspirations. I have been a little overwhelmed because my expertise is not in cytology. I may follow the protocol of the laboratory for staining these slides, but I really don't have the knowledge of how to troubleshoot a problem in cytology. For now I have been doing a good job, but I recently had a problem with the staining, because the person who trained me did not gave me clear instructions about when to change the solutions so, I did not changed the H20 saline frequently. I tough it was once a week, but then after making this mistake they told me it was like 3 times a week because this solution gets dirty very quickly and can affect the quality of the details of the stains, (they told me this too late because I already stained a whole rack of slides). Now I have to find some info on cytology so that I can understand this field better and stay competitive because in my country you have to do everything, unlike in the U.S where histotech do histology, cytotech do cytology and the pathologist assistant only do the grossing, in here there are histotech that have to do all. Now, to the frozen sections. I still have not performed a real frozen section by myself yet. I have just practiced using little pieces of chicken and I have gotten good sections but, I need to practice with real tissue because not all the tissues are the same and some are very difficult to cut. I have more experience cutting sections with the microtome, embedding blocks, doing routine H&E stains. I have not done an special stain yet, but I have knowledge of all of them because histotech school and the ASCP prepared me to know them, but I have little experience in frozen sectioning. I have the knowledge but not the practice. How ironic is life, I prepared myself 100% for histology and now I am doing things that I though I would never do. Do you have similar experiences? Could you provide me advice or good resources about cytology and frozen sections? Thanks From pruegg <@t> ihctech.net Sat Mar 13 09:57:13 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Mar 13 09:58:12 2010 Subject: [Histonet] Block patient IDs In-Reply-To: <731941C266951A47BEF11E5EFAAED9C90196E0DF@nsmvexch01.northside.local> References: <731941C266951A47BEF11E5EFAAED9C90196E0DF@nsmvexch01.northside.local> Message-ID: <55A43B14418542E2948101A6DFE7B0CA@prueggihctechlt> The new derm path lab I am associated with built their own computerized specimen tracking system and I am pretty sure that the same multi unique identifiers that are printed on the slides are printed on the blocks. They have leica slide and block printers connected to accessioning and grossing by touch screen computers and I believe they are labeled the same. If the block labeling and slide labeling are integrated why not keep them the same? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Brown Sent: Friday, March 12, 2010 9:00 AM To: Nails, Felton; Anne van Binsbergen; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs I think you will find that CAP is asking if 2 patient identifiers are used throughout the entire process. We engrave the blocks, slides and a computer generated block count log that uses 2 patient identifiers. Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, March 12, 2010 10:55 AM To: 'Anne van Binsbergen'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs It is my understand that the two patient identifier applies to the completed slide not the block. You can verify this by looking at the CAP Checklist for anatomical pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Friday, March 12, 2010 9:42 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Block patient IDs A question for all you CAP fundis: how many patient Identifiers are needed on each paraffin block We currently just write the unique, LIS generated number on the block face, but have recently been advised that CAP and JCIA require not one, but TWO patient IDS on the block Comments please -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. 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There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Mar 13 09:59:20 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Mar 13 10:00:18 2010 Subject: SPAM-LOW: [Histonet] H. pylori on Rhesus macaque tissue In-Reply-To: <582736991003111408x1b689771m24f87bc6cd0cd65b@mail.gmail.com> References: <582736991003111408x1b689771m24f87bc6cd0cd65b@mail.gmail.com> Message-ID: I agree Amos, the only thing you need to be concerned about is if the detection being used somehow binds non specifically to the monkey tissue and not the human. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Thursday, March 11, 2010 3:08 PM To: zerfasp@ors.od.nih.gov; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] H. pylori on Rhesus macaque tissue Hi, I am sure this will work. Granted I haven't tried it... The antibody is directed against the bug, the H. pylori bacteria, not against a product of the human it exists in. So it shouldn't matter where the bug is, it should have the epitope the antibody binds with. I do Hepatitis B on non human tissue all the time and it works fine. Good luck, (and let me know for sure how it goes) Amos Brooks Message: 9 Date: Thu, 11 Mar 2010 11:25:16 -0500 From: "Zerfas, Patricia (NIH/OD/ORS) [E]" Subject: [Histonet] H. pylori on Rhesus macaque tissue To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear List servers, Did anyone try this antibody on Rhesus macaque tissue? Do you have a protocol? How were the results? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Mar 13 10:02:48 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Mar 13 10:03:48 2010 Subject: SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody In-Reply-To: References: Message-ID: Is the Perma Red for hrp? If you switch to an alk.phos detection system you could use fast red which is much redder than AEC. Dako sells ap/red detection systems, but I prefer to use Vector Red for AP. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Friday, March 12, 2010 6:23 AM To: Gareth Blaeuer Davis; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody We use Diagnostic Biosystems Perma Red with the Dako system. www.dbiosys.com Works very well Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gareth Blaeuer Davis [gareth.davis@hotmail.com] Sent: Thursday, March 11, 2010 7:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Red Chromogen for MITF antibody Hi, Does anyone do MITF immunostains with a Red Chromogen. We are using a Dako autostainer, and in the past have used AEC chromogen on MITF. Our pathologist doesn't like it, because it doesn't get red enough. We would like something we could use with current protocols on the Dako, but can't find anything. Any suggestions? Gareth Blaeuer Davis, HT, BS. Pathology Associates of St. Thomas Nashville, Tn 37205 615-298-4100 _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/____________________________ ___________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Mar 13 10:09:25 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Mar 13 10:10:22 2010 Subject: SPAM-LOW: [Histonet] Re: Formalin vs alcoholic formalin In-Reply-To: References: Message-ID: <3C001778F6504D36A05868F6A45357B6@prueggihctechlt> Her2 loves alcohol and the use of it can cause false positive results. Even how the formalin is buffered matters, use only phosphate neutral buffered formalin. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, March 11, 2010 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: Formalin vs alcoholic formalin William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska asks: >>Help! I think I know the answer, but need some rapid clarification. Is an alcohol-formalin fixative acceptable for use in breast tissue, or does it need to be an aqueous 10% NBF?<< According to the FDA, the required fixation for the HER2 immunostain on breast tissue is neutral buffered formalin - accept no substitutes! I agree with them. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raestask <@t> grics.net Sat Mar 13 10:21:29 2010 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Sat Mar 13 10:21:21 2010 Subject: [Histonet] Her-2 Controls Message-ID: <299EA52854A745D7BF72D8C91534B595@your4105e587b6> Morning All, My chief pathologist has asked the following question. For all of those doing Her-2 Neu staining for scoring with the Vias system. How many control slides do you cut in advance and how long are they viable if kept refrigerated? Thanks in advance, Rae Ann Staskiewicz From pruegg <@t> ihctech.net Sat Mar 13 10:33:21 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Mar 13 10:34:24 2010 Subject: [Histonet] Histo Stories In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4D27A3ABA5D847659BCE3A1B104CF8D8@prueggihctechlt> My love for histology has allowed me to develop into one of the educators in the field. Now that I am retired from my career job at the University I started my own small histopathology services business with the opportunity to take on "histotechs in training". I have one already registered as an HT another qualified to sit for the registry, plus two more in training and will be ready to do that soon. Next week I have 60 local HS students visiting my lab in the BioScience Park Center. I give them a tour to show them what we do and pass out the "Histology Careers" flyer provided by NSH. There are several start up Biotech companies in my building but our director who hosts these kinds of tours all the time, says the kids always like visiting my histology lab the best. I always make it touchy feely and pass around a rubber rat brain I got from one of the vendor's and we do demonstrate grossing, embedding and sectioning. I think that one of the most important things is to give them that flyer to take home because it has my contact as well as NSH's on it. I get calls back from these kids all the time and have even had a few of them work for me as lab assistants and one was an intern for the summer. Histology is easy to get them excited about because it is so interesting and different to them. The teachers really like the idea that there is something kids with an associates degree can do because not all of them have an opportunity to get a BS degree or go to graduate school. I am trying to retire a second time but it is hard to leave something you love doing so much. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Turner, Mark Sent: Thursday, March 11, 2010 12:25 PM To: Breeden, Sara; histonet Subject: RE: [Histonet] Histo Stories I began working in a lab in Northern Kentucky right out of high school as a Diener, washing glassware and assisting with autopsies. During the slow times I would hang out in Histology, watching the one tech in the building work her magic. After a year or so the lab director decided to add another tech and they agreed to train me. After a few years of OJT I sat for the exam and passed it with flying colors. I continued my education, eventually getting a PhD, but I am so glad I stumbled into the Histology field. I, too, had no idea what Histotechnology was when I was in high school, but in the edited words of Garret Morris "Histology been berry, berry good to me!" Mark Turner, HT (ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Thursday, March 11, 2010 7:59 AM To: Breeden, Sara Cc: histonet Subject: Re: [Histonet] Histo Stories Sally, I didn't get a chance to answer yesterday - I went to a high school and spoke about my favorite topic - histotechnology! Anyway, I started out in microbiology and then worked in a doctor's office lab doing routine chemistry and hematology and when we moved to a small town in Iowa the only position in the lab that became available was in histology. I was playing bridge with the histotech and he mentioned the opening so I went in and interviewed, got the job and never have looked back. So I sort of fell into this profession as did many others. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Mar 11, 2010, at 6:09 AM, Breeden, Sara wrote: > Thanks to everyone that sent their Story of How I Ended Up Doing This > Histology Thing! I have gotten 50 or more replies! The one thing > that > strikes me is how many of us went into this profession without a clue! > With all the opportunities to recruit future histologists, this > Histology Day idea is a good start. On the original subject, I'm > planning to make one document out of all the replies and - WITH > PERMISSION - attach your name to the answers. If you do NOT want your > submission listed because you want to remain anonymous, you must let > me > know ASAP. Send to: nmhisto@comcast.net. Thanks for your stories! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kevinpe <@t> mail.med.upenn.edu Sat Mar 13 13:15:12 2010 From: kevinpe <@t> mail.med.upenn.edu (Kevin Egan) Date: Sat Mar 13 13:15:26 2010 Subject: [Histonet] IHC on 3 year fixed tissue? Message-ID: <4B9BE440.1020803@upenn.edu> Hello Histonetters, I've been asked to perform anti-GFP IHC on some mouse bones which have been sitting in NBF for 3-4 years. My Abcam rabbit polyclonal gets great results in GFP mouse control tissue, but I can't seem to get any results out of these bones. I've tried citrate buffer HIER using autoclave, hotplate, and 65 degree water batch over night, but my signal is non-existent (or really the noise is so huge you can't distinguish).Do the great minds of histonet have any ideas on how to achieve good staining? Is enzymatic epitope retrieval worth a try? Does anyone have a time machine I can use to stop my predecessors from just sticking the bones in NBF and forgetting about them for years? This listserv has helped me countless times, I am truly appreciative of the knowledge and experience available on Histonet. Thanks, Kevin From baumannr <@t> med.uni-marburg.de Sat Mar 13 14:41:58 2010 From: baumannr <@t> med.uni-marburg.de (baumannr@med.uni-marburg.de) Date: Sat Mar 13 14:42:03 2010 Subject: [Histonet] Histonet Message-ID: <20100313214158.xyuwbumfk84g0s8k@webmail.med.uni-marburg.de> dear Val, first my english is not good. I can understand....but the grammar! My way is similar- after studies I wanted to work in microbiologie. But I?ve got a job in Pathologie. First time I was alone in the laboratorie.I had to manage the routine...cutting,staining,preparation and so on...and that was at the beginning after my studies, I had no experiances! Sometimes I thought " OH GOD " but I think, this is the best way to learn! I have made my mistakes! -but I?ve learned.I?ve never made the same mistakes two times! Now I do my job about 36 years and I teach students in histotechnologie. Val, I think there is no problem to make a mistake at the beginning! For your " beginning " I wish you good luck. Best wishes Renate From rjbuesa <@t> yahoo.com Sat Mar 13 17:17:49 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 13 17:18:53 2010 Subject: [Histonet] IHC on 3 year fixed tissue? In-Reply-To: <4B9BE440.1020803@upenn.edu> Message-ID: <706114.82676.qm@web65710.mail.ac4.yahoo.com> I have to imagine that you are doing the HIER in the FFPE sections? If you have used autoclave unsuccessfully it seems that the epitopes are so cross-linked that they do not respond to the treatment. I would try a piece of fixed tissue before processing, cut it in a very thin slice (about?2 mm maximum)?and heat it in distilled water at 65?C for 30 minutes. Wah it thoroughly and process it as usual. After that, prepare the sections and HIER them?in citrate buffer in a steamer and proceed as usual. By doing this you will have to steps were the cross-linkages area going to be weaken and perhaps you will get some acceptable reaction. Ren? J. --- On Sat, 3/13/10, Kevin Egan wrote: From: Kevin Egan Subject: [Histonet] IHC on 3 year fixed tissue? To: histonet@lists.utsouthwestern.edu Date: Saturday, March 13, 2010, 2:15 PM Hello Histonetters, I've been asked to perform anti-GFP IHC on some mouse bones which have been sitting in NBF for 3-4 years. My Abcam rabbit polyclonal gets great results in GFP mouse control tissue, but I can't seem to get any results out of these bones. I've tried citrate buffer HIER using autoclave, hotplate, and 65 degree water batch over night, but my signal is non-existent (or really the noise is so huge you can't distinguish).Do the great minds of histonet have any ideas on how to achieve good staining? Is enzymatic epitope retrieval worth? a try? Does anyone have a time machine I can use to stop my predecessors from just sticking the bones in NBF and forgetting about them for years? This listserv has helped me countless times, I am truly appreciative of the knowledge and experience available on Histonet. Thanks, Kevin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From detmar <@t> lunenfeld.ca Sat Mar 13 21:18:17 2010 From: detmar <@t> lunenfeld.ca (Jacqui Detmar) Date: Sat Mar 13 21:18:32 2010 Subject: [Histonet] IHC on 3 year fixed tissue? References: <706114.82676.qm@web65710.mail.ac4.yahoo.com> Message-ID: Hey there. I have done IHC on mouse placentae that have been fixed in NBF for over 2 years and it took me 5-10 minutes of ProK (10 ug/ml in PBS) followed by 60 minutes of HIER in 10 mM citrate buffer (made sure PBS was at pH 6) in a veggie steamer. I had previously done this IHC in a veggie steamer for only 25 minutes in 10 mM citrate buffer (pH 6) for mouse placentae that had only been fixed for 24 hours in NBF. The antigen was a nuclear receptor (AhR); I have found nuclear antigens to be slightly more difficult to retrieve...at least for mouse placenta! Note that the 2-year-fixed tissue did NOT react positively until the 60-minute mark after HIER, although I tested at 25, 35 and 50 minutes. Only 60 minutes HIER plus enzyme retrieval worked, even though "quick-fixed" tissue worked simply with 25 minutes HIER. Definitely worth a try, if you're desperate. It took me over a year to get this **#&%*#&%** tissue working! Also, used a 1:100 dilution instead of my normal 1:500 dilution of the antibody, so you might want to titrate the antibody, too. Am I being lucid? Have just come home from a most excellent dinner party! Perhaps not such a good idea to give histo-advice on a Saturday night....but I deeply sympathized with your plight . If you have any questions, please feel free to contact me personally, Kevin, either on gmail or at my SLRI account. Jacqui Jacqui Detmar Work phone: 1-416-646-0223 Work fax: 1-416-862-9696 Cell phone: 1-647-273-8735 jacquidetmar@gmail.com ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Sat 3/13/2010 6:17 PM To: histonet@lists.utsouthwestern.edu; Kevin Egan Subject: Re: [Histonet] IHC on 3 year fixed tissue? I have to imagine that you are doing the HIER in the FFPE sections? If you have used autoclave unsuccessfully it seems that the epitopes are so cross-linked that they do not respond to the treatment. I would try a piece of fixed tissue before processing, cut it in a very thin slice (about 2 mm maximum) and heat it in distilled water at 65?C for 30 minutes. Wah it thoroughly and process it as usual. After that, prepare the sections and HIER them in citrate buffer in a steamer and proceed as usual. By doing this you will have to steps were the cross-linkages area going to be weaken and perhaps you will get some acceptable reaction. Ren? J. --- On Sat, 3/13/10, Kevin Egan wrote: From: Kevin Egan Subject: [Histonet] IHC on 3 year fixed tissue? To: histonet@lists.utsouthwestern.edu Date: Saturday, March 13, 2010, 2:15 PM Hello Histonetters, I've been asked to perform anti-GFP IHC on some mouse bones which have been sitting in NBF for 3-4 years. My Abcam rabbit polyclonal gets great results in GFP mouse control tissue, but I can't seem to get any results out of these bones. I've tried citrate buffer HIER using autoclave, hotplate, and 65 degree water batch over night, but my signal is non-existent (or really the noise is so huge you can't distinguish).Do the great minds of histonet have any ideas on how to achieve good staining? Is enzymatic epitope retrieval worth a try? Does anyone have a time machine I can use to stop my predecessors from just sticking the bones in NBF and forgetting about them for years? This listserv has helped me countless times, I am truly appreciative of the knowledge and experience available on Histonet. Thanks, Kevin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From susanbachus <@t> verizon.net Sun Mar 14 01:28:12 2010 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Sun Mar 14 01:28:23 2010 Subject: [Histonet] in situ hybridization histochemistry question about radioisotope In-Reply-To: <65C621FE47744D99BDA95D8A3F5B49FE@OwnerPC> References: <4D14F0FC9316DD41972D5F03C070908B02E46F37@nmdamailsvr.nmda.ad.nmsu.edu> <65C621FE47744D99BDA95D8A3F5B49FE@OwnerPC> Message-ID: <58C8F3C807724CEA82DB1846B70D1928@OwnerPC> I have routinely seen that we obtain slightly different results (like 10-20% variance) in using 35S dATP for TdT-catalyzed tailing reactions of oligonucleotides from month to month as we use different batches (made fresh monthly) of the 35S dATP. But on a few occasions over the years we have found dramatically reduced results with a particular lot. This happened again in February, and I confirmed by comparing directly between the Feb. & March lots in March that the results with the Feb. lot were half that of the March batch (of course, given the half-life of 35S, they would be expected to be down some, but not by half!). The company was good about believing me and not charging us for the March "replacement" batch, but they have no idea what could cause this to happen occasionally. They check the specific activity, pH, purity, etc. and run a "bio-assay" that is a binding assay each month, but were not set up to test the enzymatic reaction. I told them that years ago when this happened it was finally determined that the DTT concentration was unusually high and interfered with the reaction, but they discounted this possibility. I guess I should just count my blessings that things are working again now (mercifully, I was able to cling to optimism, based on past experience, that the isotope was the problem, until I could confirm this myself), but it's very frustrating that the company is not motivated to check the efficacy of the item for this particular application (which I would imagine a lot of customers use it for) each month, or to figure out what causes this to happen on occasion (so as to avoid it!)--we lose a lot of time and other expensive reagents (e.g. the TdT) each time this happens. I also don't know how to interpret it--I know that as the enzyme begins to degrade it just means that the tails are shorter, which I can compensate for with a longer film exposure, but I have no idea what's going on when the isotope causes the problem & am afraid to use those batches. Does anyone else using ISH have any ideas about what causes this variability? grateful for any thoughts, Susan -------------- next part -------------- No virus found in this outgoing message. Checked by AVG - www.avg.com Version: 8.5.436 / Virus Database: 271.1.1/2745 - Release Date: 03/13/10 21:51:00 From vrodriguez10 <@t> gmail.com Sun Mar 14 07:45:51 2010 From: vrodriguez10 <@t> gmail.com (Valerie Rodriguez) Date: Sun Mar 14 07:46:17 2010 Subject: [Histonet] Hello everyone, I am new to this forum Message-ID: Val, the life is very funny. I graduated in Chemical Engineering at the University of Pisa. I'm Italian. I worked in a mechanical company: Fiat. Since I was 15teen my greater hobby was the microscope. Now I am a pensioner and my hobby goes on with Histology. On FaceBook (FB) I have a lot of histologist friends. If you join me on FB (massimo.tosi_m@libero.it) I think they could help you. Thank you. With my Best Regards, Massimo Tosi Yes, life is funny, considering that I first wanted to be a cytotech, but I could not, so I ended up being an histotech and ended up liking histology way more, and now I am working in a lab that focus more in cytopathology. There is no microtomes, embedding machines, just a cryostat, and frozen section stains and the cytology stains. I am not doing the smears yet, another tech is doing it, but soon they will train me how to assist the pathologist in a fine needle aspiration just in case the other tech can't do it. I just stain the PAP slides, coverslip them, organize them etc, but still it is good to know cytology so that when something wrong happens, I can troubleshoot the problem. The people from the lab assumes that histology and cytology are very alike but they are not. One of them told me that you stains slides the same, and that got me dumbfounded because in histology you don't use H20 saline as a dehydrant solution. If I were a cytotech I would knew already that I had to change the H20 saline frequently because is the solution that gets more dirty after staining a few racks of slides. In cytology hematoxylin is used, but orange,and EA, are not used in histology. The bluing solution is almost the same, it used to be lithium carbonate in the lab but seems they changed it to another chemical. They use 8 containers of alcohol 95%, and 3 of 99% when I compared that to the H&e protocol of my histology textbook cytology uses a looooot more alcohol, so the solutions are not the same. Plus you must be very careful with the slides because they don't have more cytology samples, unlike histology if a slide breaks you can look for that block of tissue and you cut a new slide, that is not possible in cytology, well in their lab, because I ask this and they told me that if I mess up a slide, then the pathologist has to obtain a new sample from the patient, so I think cytology is a little more difficult than histology for this reason. Seems that these two careers are slowly getting incorporated. In my country seems that this happen long time ago, but in the U.S I think cytology and histology is going to become one profession. The new Frieda Carson Histotechnology textbook has a new chapter about cytoprepatation. This book appeared in amazon stores online like a month after I graduated histotech school, so too bad it did not came before because I could have bough it instead of the old version I have. I will ask a question about this book in the forum later. We'll thanks for the reply. From vrodriguez10 <@t> gmail.com Sun Mar 14 08:00:32 2010 From: vrodriguez10 <@t> gmail.com (Valerie Rodriguez) Date: Sun Mar 14 08:00:59 2010 Subject: [Histonet] Question about new Frieda Carson book Message-ID: I have a question about the new Frieda Carson book: Histotechnology: A Self-Instructional Text 3rd edition. In this book there is a new chapter about cytopreparation techniques. I will like to know if this chapter teaches you how to troubleshoot problems in the staining of the slides, how to do fine needle aspirations and other techniques in cytology, or it just gives you a brief review of how to prepare cytology?????. I have the previous version, the new book came right after I graduate histotech school. Is it worth to buy the new book? Someone told me the pictures and text of all the other chapters have not changed, just the new cytology chapter. My lab is concentrated more in fine needle aspirations (cytology) than histology so I think it will be good for me to buy the new edition since is like a chapter made just for histotech so that they can undersand cytology better, but since is new is a little pricey. I may wait until the price is a little lower. Frieda's book is very excelent in preparing histotechs on how to troubleshoot problems, but I will like to find a book like that but for cytotechnology. What are your opinions???? From vrodriguez10 <@t> gmail.com Sun Mar 14 11:14:28 2010 From: vrodriguez10 <@t> gmail.com (Valerie Rodriguez) Date: Sun Mar 14 11:14:54 2010 Subject: [Histonet] Histonet Message-ID: Thank You very much.English is not my first language also. My native language is Spanish, so I also make grammar mistakes once in a while :). I meet an histotechnician who first got a bachelor degree in microbiology but could not get a job, and he started to volunteer in a pathology lab. All he did was simple tasks just as labeling and filing slides, then he got trained in histology since he showed interest. Since he has no degree in histotech only the HT licence of PR he was not payed very well so he decided to pursue his studies in cytotechnology, now I think he will graduate this year during the summer. Sometimes we prepared ourselves for a specialization and ended up something entirely different. That's how life is, but as you say the best thing is to prepare yourself for everything to say competitive in the clinical laboratory science field. There are labs in Puerto Rico where even the histotech has to work in the reception area!. Your story is inspiring, this is my first time working, so I have a lof of pressure, since I think I am the only HTL in Puerto Rico who got a histotech degree in the U.S, the rest here are just trained. Very few also have the ASCP certification, the government of puerto rico provide their own licenses. A university in Puerto Rico used to offer a 1 program course of histotechnology but I think they stopped the program in the early 90's. Since I got educated in the U.S the employees in the lab expect me to know everything. I have no work experience, but I had my internship in the U.S, then I worked as a volunteer in two diffent pathology laboratories in Puerto Rico, the way they organize their system is very different, so in my first two weeks it has taken time to understand their system. I started off good but I have made my mistakes but from your mistakes you learn. I hope I keep pursuing this career for a long time like you and have the chance to teach students in a histotech school. I wish I could open one here, it is very important. We'll take care. From vrodriguez10 <@t> gmail.com Sun Mar 14 11:23:53 2010 From: vrodriguez10 <@t> gmail.com (Valerie Rodriguez) Date: Sun Mar 14 11:24:18 2010 Subject: [Histonet] Question about new Frieda Carson book Message-ID: No, I don't have the book, for how much are you selling it? I am looking for a very economic book, not too expensive, since cytotech is not really my profession, I just want a guide just to have some knowledge of cytotech. I am also interested in the new edition of frieda textbook but if your book is more complete then it may be better. I don't need to know about diagnostic cytopathology just the technical part. Thanks From stevenk <@t> med.usyd.edu.au Sun Mar 14 17:33:06 2010 From: stevenk <@t> med.usyd.edu.au (Stephen Kum Jew) Date: Sun Mar 14 17:34:11 2010 Subject: [Histonet] Re: Gas in Biohazard hood - safety issue In-Reply-To: <20100225180620.89E693EAF89@seive.med.usyd.edu.au> Message-ID: One of our research labs has had tissue culture contamination problems. One solution is to install a gas connection to flame the glass pipettes to improve sterility. Apart from the usual problems of upsetting the air flow within the biohazard cabinet, I was wondering if the unburnt gas will build up to a dangerous level within a closed recirculating air situation. I have not found any incidents on the web about gas explosions in hoods. I am waiting a reply from the manufacturer. Has anyone had experience on this? Thanks Stephen From cbarone <@t> NEMOURS.ORG Sun Mar 14 18:06:36 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Sun Mar 14 18:06:41 2010 Subject: [Histonet] Proficiency testing Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7AE7@wlmmsx01.nemours.org> I am looking for proficiency testing for my staff for enzyme histochemistry. Presently we have been using a pathologist (not related to our organization). He is planning on retiring in June. We are a CLIA lab. I have noted EHC is not a speciality offered by HQIP. What are other EHC labs doing if you are not CAP....and just doing this specialty. We are a research core offering EHC and have been doing EHC since 1982. At present we have been CLIA certified for 12 years with no dificiencies....and want to keep it that way. Is there another lab willing to do proficiency testing by reviewing each other's slides, and does anyone know if that is acceptable, under CLIA? Can any Histonetter's help us with this one! CB From Tony_Reilly <@t> health.qld.gov.au Sun Mar 14 18:36:42 2010 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Sun Mar 14 18:41:12 2010 Subject: [Histonet] alcian blue van gieson stain In-Reply-To: References: Message-ID: <4B9DFFA9.471C.0039.0@health.qld.gov.au> Hi Margaret You perform the Alcian Blue first but as with Movatt's pentachrome you need to treat the sections after AB with alcoholic ammonia for an hour to convert the AB to Monastral Blue as the AB is soluable in picric acid and the Monastral blue resistant. Let me know if you need a method regards Tony Tony Reilly Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Perry, Margaret" 13/03/2010 12:30 am >>> We want to try the double stain alcian blue Van gieson. Do you stain for the alcian blue first and then the Van gieson? We do both stains but I am unsure of how to put them together. Margaret Perry South Dakota State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From matt <@t> techoneweb.com Sun Mar 14 19:31:46 2010 From: matt <@t> techoneweb.com (Matthew Mincer) Date: Sun Mar 14 19:29:33 2010 Subject: [Histonet] Peloris Message-ID: <4B9D7FF2.8000307@techoneweb.com> Hey All, Does anyone know where I can borrow / rent a Peloris for a month? I have found a guy who is willing to train my techs how to service it but I would prefer do it in my shop rather than a lab. Peace Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Mar 15 07:12:36 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Mar 15 07:13:14 2010 Subject: SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody In-Reply-To: References: , Message-ID: The perma red is for HRP or alk phos. Depends on your detection polymer. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: Patsy Ruegg [pruegg@ihctech.net] Sent: Saturday, March 13, 2010 11:02 AM To: McMahon, Loralee A; 'Gareth Blaeuer Davis'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody Is the Perma Red for hrp? If you switch to an alk.phos detection system you could use fast red which is much redder than AEC. Dako sells ap/red detection systems, but I prefer to use Vector Red for AP. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Friday, March 12, 2010 6:23 AM To: Gareth Blaeuer Davis; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody We use Diagnostic Biosystems Perma Red with the Dako system. www.dbiosys.com Works very well Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gareth Blaeuer Davis [gareth.davis@hotmail.com] Sent: Thursday, March 11, 2010 7:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Red Chromogen for MITF antibody Hi, Does anyone do MITF immunostains with a Red Chromogen. We are using a Dako autostainer, and in the past have used AEC chromogen on MITF. Our pathologist doesn't like it, because it doesn't get red enough. We would like something we could use with current protocols on the Dako, but can't find anything. Any suggestions? Gareth Blaeuer Davis, HT, BS. Pathology Associates of St. Thomas Nashville, Tn 37205 615-298-4100 _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/____________________________ ___________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MadaryJ <@t> MedImmune.com Mon Mar 15 08:46:11 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Mar 15 08:46:24 2010 Subject: [Histonet] Problem with section frozen human eye lens In-Reply-To: References: Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A13015B4B75@MD1EV002.medimmune.com> Any netters have some idea about sectioning human eye without losing the lens part way through? A softening treatment that will allow for frozen sectioning, a special temperature? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, March 14, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC on 3 year fixed tissue? (Kevin Egan) 2. Histonet (baumannr@med.uni-marburg.de) 3. Re: IHC on 3 year fixed tissue? (Rene J Buesa) 4. RE: IHC on 3 year fixed tissue? (Jacqui Detmar) 5. in situ hybridization histochemistry question about radioisotope (Susan Bachus) 6. Hello everyone, I am new to this forum (Valerie Rodriguez) 7. Question about new Frieda Carson book (Valerie Rodriguez) 8. Histonet (Valerie Rodriguez) 9. Question about new Frieda Carson book (Valerie Rodriguez) ---------------------------------------------------------------------- Message: 1 Date: Sat, 13 Mar 2010 14:15:12 -0500 From: Kevin Egan Subject: [Histonet] IHC on 3 year fixed tissue? To: histonet@lists.utsouthwestern.edu Message-ID: <4B9BE440.1020803@upenn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello Histonetters, I've been asked to perform anti-GFP IHC on some mouse bones which have been sitting in NBF for 3-4 years. My Abcam rabbit polyclonal gets great results in GFP mouse control tissue, but I can't seem to get any results out of these bones. I've tried citrate buffer HIER using autoclave, hotplate, and 65 degree water batch over night, but my signal is non-existent (or really the noise is so huge you can't distinguish).Do the great minds of histonet have any ideas on how to achieve good staining? Is enzymatic epitope retrieval worth a try? Does anyone have a time machine I can use to stop my predecessors from just sticking the bones in NBF and forgetting about them for years? This listserv has helped me countless times, I am truly appreciative of the knowledge and experience available on Histonet. Thanks, Kevin ------------------------------ Message: 2 Date: Sat, 13 Mar 2010 21:41:58 +0100 From: baumannr@med.uni-marburg.de Subject: [Histonet] Histonet To: histonet@lists.utsouthwestern.edu Message-ID: <20100313214158.xyuwbumfk84g0s8k@webmail.med.uni-marburg.de> Content-Type: text/plain; charset=ISO-8859-1; DelSp="Yes"; format="flowed" dear Val, first my english is not good. I can understand....but the grammar! My way is similar- after studies I wanted to work in microbiologie. But I?ve got a job in Pathologie. First time I was alone in the laboratorie.I had to manage the routine...cutting,staining,preparation and so on...and that was at the beginning after my studies, I had no experiances! Sometimes I thought " OH GOD " but I think, this is the best way to learn! I have made my mistakes! -but I?ve learned.I?ve never made the same mistakes two times! Now I do my job about 36 years and I teach students in histotechnologie. Val, I think there is no problem to make a mistake at the beginning! For your " beginning " I wish you good luck. Best wishes Renate ------------------------------ Message: 3 Date: Sat, 13 Mar 2010 15:17:49 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] IHC on 3 year fixed tissue? To: histonet@lists.utsouthwestern.edu, Kevin Egan Message-ID: <706114.82676.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I have to imagine that you are doing the HIER in the FFPE sections? If you have used autoclave unsuccessfully it seems that the epitopes are so cross-linked that they do not respond to the treatment. I would try a piece of fixed tissue before processing, cut it in a very thin slice (about?2 mm maximum)?and heat it in distilled water at 65?C for 30 minutes. Wah it thoroughly and process it as usual. After that, prepare the sections and HIER them?in citrate buffer in a steamer and proceed as usual. By doing this you will have to steps were the cross-linkages area going to be weaken and perhaps you will get some acceptable reaction. Ren? J. --- On Sat, 3/13/10, Kevin Egan wrote: From: Kevin Egan Subject: [Histonet] IHC on 3 year fixed tissue? To: histonet@lists.utsouthwestern.edu Date: Saturday, March 13, 2010, 2:15 PM Hello Histonetters, I've been asked to perform anti-GFP IHC on some mouse bones which have been sitting in NBF for 3-4 years. My Abcam rabbit polyclonal gets great results in GFP mouse control tissue, but I can't seem to get any results out of these bones. I've tried citrate buffer HIER using autoclave, hotplate, and 65 degree water batch over night, but my signal is non-existent (or really the noise is so huge you can't distinguish).Do the great minds of histonet have any ideas on how to achieve good staining? Is enzymatic epitope retrieval worth? a try? Does anyone have a time machine I can use to stop my predecessors from just sticking the bones in NBF and forgetting about them for years? This listserv has helped me countless times, I am truly appreciative of the knowledge and experience available on Histonet. Thanks, Kevin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sat, 13 Mar 2010 22:18:17 -0500 From: "Jacqui Detmar" Subject: RE: [Histonet] IHC on 3 year fixed tissue? To: "Rene J Buesa" , , "Kevin Egan" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hey there. I have done IHC on mouse placentae that have been fixed in NBF for over 2 years and it took me 5-10 minutes of ProK (10 ug/ml in PBS) followed by 60 minutes of HIER in 10 mM citrate buffer (made sure PBS was at pH 6) in a veggie steamer. I had previously done this IHC in a veggie steamer for only 25 minutes in 10 mM citrate buffer (pH 6) for mouse placentae that had only been fixed for 24 hours in NBF. The antigen was a nuclear receptor (AhR); I have found nuclear antigens to be slightly more difficult to retrieve...at least for mouse placenta! Note that the 2-year-fixed tissue did NOT react positively until the 60-minute mark after HIER, although I tested at 25, 35 and 50 minutes. Only 60 minutes HIER plus enzyme retrieval worked, even though "quick-fixed" tissue worked simply with 25 minutes HIER. Definitely worth a try, if you're desperate. It took me over a year to get this **#&%*#&%** tissue working! Also, used a 1:100 dilution instead of my normal 1:500 dilution of the antibody, so you might want to titrate the antibody, too. Am I being lucid? Have just come home from a most excellent dinner party! Perhaps not such a good idea to give histo-advice on a Saturday night....but I deeply sympathized with your plight . If you have any questions, please feel free to contact me personally, Kevin, either on gmail or at my SLRI account. Jacqui Jacqui Detmar Work phone: 1-416-646-0223 Work fax: 1-416-862-9696 Cell phone: 1-647-273-8735 jacquidetmar@gmail.com ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Sat 3/13/2010 6:17 PM To: histonet@lists.utsouthwestern.edu; Kevin Egan Subject: Re: [Histonet] IHC on 3 year fixed tissue? I have to imagine that you are doing the HIER in the FFPE sections? If you have used autoclave unsuccessfully it seems that the epitopes are so cross-linked that they do not respond to the treatment. I would try a piece of fixed tissue before processing, cut it in a very thin slice (about 2 mm maximum) and heat it in distilled water at 65?C for 30 minutes. Wah it thoroughly and process it as usual. After that, prepare the sections and HIER them in citrate buffer in a steamer and proceed as usual. By doing this you will have to steps were the cross-linkages area going to be weaken and perhaps you will get some acceptable reaction. Ren? J. --- On Sat, 3/13/10, Kevin Egan wrote: From: Kevin Egan Subject: [Histonet] IHC on 3 year fixed tissue? To: histonet@lists.utsouthwestern.edu Date: Saturday, March 13, 2010, 2:15 PM Hello Histonetters, I've been asked to perform anti-GFP IHC on some mouse bones which have been sitting in NBF for 3-4 years. My Abcam rabbit polyclonal gets great results in GFP mouse control tissue, but I can't seem to get any results out of these bones. I've tried citrate buffer HIER using autoclave, hotplate, and 65 degree water batch over night, but my signal is non-existent (or really the noise is so huge you can't distinguish).Do the great minds of histonet have any ideas on how to achieve good staining? Is enzymatic epitope retrieval worth a try? Does anyone have a time machine I can use to stop my predecessors from just sticking the bones in NBF and forgetting about them for years? This listserv has helped me countless times, I am truly appreciative of the knowledge and experience available on Histonet. Thanks, Kevin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Sun, 14 Mar 2010 02:28:12 -0500 From: "Susan Bachus" Subject: [Histonet] in situ hybridization histochemistry question about radioisotope To: "histonet" Message-ID: <58C8F3C807724CEA82DB1846B70D1928@OwnerPC> Content-Type: text/plain; charset="iso-8859-1" I have routinely seen that we obtain slightly different results (like 10-20% variance) in using 35S dATP for TdT-catalyzed tailing reactions of oligonucleotides from month to month as we use different batches (made fresh monthly) of the 35S dATP. But on a few occasions over the years we have found dramatically reduced results with a particular lot. This happened again in February, and I confirmed by comparing directly between the Feb. & March lots in March that the results with the Feb. lot were half that of the March batch (of course, given the half-life of 35S, they would be expected to be down some, but not by half!). The company was good about believing me and not charging us for the March "replacement" batch, but they have no idea what could cause this to happen occasionally. They check the specific activity, pH, purity, etc. and run a "bio-assay" that is a binding assay each month, but were not set up to test the enzymatic reaction. I told them that years ago when this happened it was finally determined that the DTT concentration was unusually high and interfered with the reaction, but they discounted this possibility. I guess I should just count my blessings that things are working again now (mercifully, I was able to cling to optimism, based on past experience, that the isotope was the problem, until I could confirm this myself), but it's very frustrating that the company is not motivated to check the efficacy of the item for this particular application (which I would imagine a lot of customers use it for) each month, or to figure out what causes this to happen on occasion (so as to avoid it!)--we lose a lot of time and other expensive reagents (e.g. the TdT) each time this happens. I also don't know how to interpret it--I know that as the enzyme begins to degrade it just means that the tails are shorter, which I can compensate for with a longer film exposure, but I have no idea what's going on when the isotope causes the problem & am afraid to use those batches. Does anyone else using ISH have any ideas about what causes this variability? grateful for any thoughts, Susan -------------- next part -------------- No virus found in this outgoing message. Checked by AVG - www.avg.com Version: 8.5.436 / Virus Database: 271.1.1/2745 - Release Date: 03/13/10 21:51:00 ------------------------------ Message: 6 Date: Sun, 14 Mar 2010 05:45:51 -0700 From: Valerie Rodriguez Subject: [Histonet] Hello everyone, I am new to this forum To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Val, the life is very funny. I graduated in Chemical Engineering at the University of Pisa. I'm Italian. I worked in a mechanical company: Fiat. Since I was 15teen my greater hobby was the microscope. Now I am a pensioner and my hobby goes on with Histology. On FaceBook (FB) I have a lot of histologist friends. If you join me on FB (massimo.tosi_m@libero.it) I think they could help you. Thank you. With my Best Regards, Massimo Tosi Yes, life is funny, considering that I first wanted to be a cytotech, but I could not, so I ended up being an histotech and ended up liking histology way more, and now I am working in a lab that focus more in cytopathology. There is no microtomes, embedding machines, just a cryostat, and frozen section stains and the cytology stains. I am not doing the smears yet, another tech is doing it, but soon they will train me how to assist the pathologist in a fine needle aspiration just in case the other tech can't do it. I just stain the PAP slides, coverslip them, organize them etc, but still it is good to know cytology so that when something wrong happens, I can troubleshoot the problem. The people from the lab assumes that histology and cytology are very alike but they are not. One of them told me that you stains slides the same, and that got me dumbfounded because in histology you don't use H20 saline as a dehydrant solution. If I were a cytotech I would knew already that I had to change the H20 saline frequently because is the solution that gets more dirty after staining a few racks of slides. In cytology hematoxylin is used, but orange,and EA, are not used in histology. The bluing solution is almost the same, it used to be lithium carbonate in the lab but seems they changed it to another chemical. They use 8 containers of alcohol 95%, and 3 of 99% when I compared that to the H&e protocol of my histology textbook cytology uses a looooot more alcohol, so the solutions are not the same. Plus you must be very careful with the slides because they don't have more cytology samples, unlike histology if a slide breaks you can look for that block of tissue and you cut a new slide, that is not possible in cytology, well in their lab, because I ask this and they told me that if I mess up a slide, then the pathologist has to obtain a new sample from the patient, so I think cytology is a little more difficult than histology for this reason. Seems that these two careers are slowly getting incorporated. In my country seems that this happen long time ago, but in the U.S I think cytology and histology is going to become one profession. The new Frieda Carson Histotechnology textbook has a new chapter about cytoprepatation. This book appeared in amazon stores online like a month after I graduated histotech school, so too bad it did not came before because I could have bough it instead of the old version I have. I will ask a question about this book in the forum later. We'll thanks for the reply. ------------------------------ Message: 7 Date: Sun, 14 Mar 2010 06:00:32 -0700 From: Valerie Rodriguez Subject: [Histonet] Question about new Frieda Carson book To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I have a question about the new Frieda Carson book: Histotechnology: A Self-Instructional Text 3rd edition. In this book there is a new chapter about cytopreparation techniques. I will like to know if this chapter teaches you how to troubleshoot problems in the staining of the slides, how to do fine needle aspirations and other techniques in cytology, or it just gives you a brief review of how to prepare cytology?????. I have the previous version, the new book came right after I graduate histotech school. Is it worth to buy the new book? Someone told me the pictures and text of all the other chapters have not changed, just the new cytology chapter. My lab is concentrated more in fine needle aspirations (cytology) than histology so I think it will be good for me to buy the new edition since is like a chapter made just for histotech so that they can undersand cytology better, but since is new is a little pricey. I may wait until the price is a little lower. Frieda's book is very excelent in preparing histotechs on how to troubleshoot problems, but I will like to find a book like that but for cytotechnology. What are your opinions???? ------------------------------ Message: 8 Date: Sun, 14 Mar 2010 09:14:28 -0700 From: Valerie Rodriguez Subject: [Histonet] Histonet To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Thank You very much.English is not my first language also. My native language is Spanish, so I also make grammar mistakes once in a while :). I meet an histotechnician who first got a bachelor degree in microbiology but could not get a job, and he started to volunteer in a pathology lab. All he did was simple tasks just as labeling and filing slides, then he got trained in histology since he showed interest. Since he has no degree in histotech only the HT licence of PR he was not payed very well so he decided to pursue his studies in cytotechnology, now I think he will graduate this year during the summer. Sometimes we prepared ourselves for a specialization and ended up something entirely different. That's how life is, but as you say the best thing is to prepare yourself for everything to say competitive in the clinical laboratory science field. There are labs in Puerto Rico where even the histotech has to work in the reception area!. Your story is inspiring, this is my first time working, so I have a lof of pressure, since I think I am the only HTL in Puerto Rico who got a histotech degree in the U.S, the rest here are just trained. Very few also have the ASCP certification, the government of puerto rico provide their own licenses. A university in Puerto Rico used to offer a 1 program course of histotechnology but I think they stopped the program in the early 90's. Since I got educated in the U.S the employees in the lab expect me to know everything. I have no work experience, but I had my internship in the U.S, then I worked as a volunteer in two diffent pathology laboratories in Puerto Rico, the way they organize their system is very different, so in my first two weeks it has taken time to understand their system. I started off good but I have made my mistakes but from your mistakes you learn. I hope I keep pursuing this career for a long time like you and have the chance to teach students in a histotech school. I wish I could open one here, it is very important. We'll take care. ------------------------------ Message: 9 Date: Sun, 14 Mar 2010 09:23:53 -0700 From: Valerie Rodriguez Subject: [Histonet] Question about new Frieda Carson book To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 No, I don't have the book, for how much are you selling it? I am looking for a very economic book, not too expensive, since cytotech is not really my profession, I just want a guide just to have some knowledge of cytotech. I am also interested in the new edition of frieda textbook but if your book is more complete then it may be better. I don't need to know about diagnostic cytopathology just the technical part. Thanks ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 20 **************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From Herrick.James <@t> mayo.edu Mon Mar 15 08:52:50 2010 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Mon Mar 15 08:52:59 2010 Subject: [Histonet] Processing Question Message-ID: <7267A64D75F58241B577876D8A885631015A9A02@msgebe41> Dear Histonet Colleagues, I am trying to develop a processing protocol for our Leica TP1020, but the first run has not been very successful. The specimen is canine femur (section lengths of 1" to 1-1/2 "). Following is a list of the processing reagents and times for my first attempt: 70% ETOH for 4 hours with vacuum 95% ETOH for 4 hours with vacuum 95% ETOH for 4 hours with vacuum 95% ETOH for 4 hours with vacuum 100% ETOH for 8 hours with vacuum 100% ETOH for 8 hours with vacuum 100% ETOH for 8 hours with vacuum 100% ETOH for 8 hours with vacuum Methyl Methacrylate (Pure) for 4 hours with vacuum Methyl Methacrylate (Pure) for 6 hours with vacuum Methyl Methacrylate (Pure) for 8 hours with vacuum Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum (specimen was placed into refrigerator overnight) Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum (specimen was placed into refrigerator overnight) Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum Methyl Methacrylate with Dibutyl Phthalate and Perkadox for 4 hours with vacuum (specimen was placed into refrigerator overnight) Specimen was removed from refrigerator and embedded in a water bath for 2 days. I cut the specimen in half in the longitudinal direction. The cortices appear to have been infiltrated fairly well, but the marrow still has some very large holes. If anyone would happen to have a protocol that works well with this type of tissue and this processor, it would be greatly appreciated if you could offer your advice and expertise as to what I should do next. I do have one more specimen still running in the processor and will probably embed it on Monday. The first specimen was started on a Monday and embedded the following Monday (approx. 8 days), so this second specimen will have been in MMA for an extra 8 days before embedding. Thank you very much for your help. Jim From e.ballarini1 <@t> campus.unimib.it Mon Mar 15 09:05:50 2010 From: e.ballarini1 <@t> campus.unimib.it (Elisa Ballarini) Date: Mon Mar 15 09:06:00 2010 Subject: [Histonet] spinal cord paraffin embedding Message-ID: Dear all, I'm quite new in morphological analysis and Histology. In my l blue staining. First we fix the spinal cord (only lumbar portion) in 4% parafolmaldehyde (4h) paraffin using a authomatic Leica ASP300 machine. When observed, the white matter of the spinal cord is full of holes and myelin seems to be absent. I guess the problem was related to the processing steps the machine, in particular the Xylol step. Is there someone who can help me sharing his/her experience on spinal cord process Any suggestion will be highly appreciated. Regards -- Dott.ssa Elisa Ballarini,PhD student Dipartimento di Neuroscie Universit? degli Studi Milano-Bicocca Via C e.ballarini1@campus.unimib.it Tel. 02-64488119 Fax. 02-64488253 From histonet.nospam <@t> vneubert.com Mon Mar 15 09:33:24 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Mon Mar 15 09:33:42 2010 Subject: [Histonet] spinal cord paraffin embedding In-Reply-To: References: Message-ID: <4B9E4534.9060301@vneubert.com> Hi, I processed small porcine spinal cord once (diameter ~3-5mm). My pathologist did not mention any abnormalities, so I guess our standard protocol for every type of tissue was fine. for 1h each: NBF 70% isopropanol ( = 2-propanol) 80% isopropanol 95% isopropanol 100% isopropanol 100% isopropanol 100% isopropanol xylene xylene paraffine paraffine If nothing else helps, maybe you want to give that a try with slightly shorter times in the alcoholic solutions, as your specimen seems to be smaller in size. > Dear all, > I'm quite new in morphological analysis and Histology. > In my l=ab we are processing rat spinal cord to perform luxol fast > blue staining. > First we fix the spinal cord (only lumbar portion) in 4% > parafolmaldehyde (4h) =y immesion, then the samples are embedded in > paraffin using a authomatic Leica ASP300 machine. When observed, the > white matter of the spinal cord is full of holes and myelin seems to > be absent. > I guess the problem was related to the processing steps=erformed by > the machine, in particular the Xylol step. > Is there someone who can help me sharing his/her experience on spinal > cord process=ng protocol? > Any suggestion will be highly appreciated. > Regards > -- > Dott.ssa Elisa Ballarini,PhD student > Dipartimento di Neuroscie=ze e Tecnologie Biomediche > Universit? degli Studi Milano-Bicocca > Via C=dore 48-20052,Monza,MB > e.ballarini1@campus.unimib.it > Tel. 02-64488119 > Fax. 02-64488253 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Am 15.03.2010 15:05, schrieb Elisa Ballarini: > Dear all, > I'm quite new in morphological analysis and Histology. > In my l=ab we are processing rat spinal cord to perform luxol fast > blue staining. > First we fix the spinal cord (only lumbar portion) in 4% > parafolmaldehyde (4h) =y immesion, then the samples are embedded in > paraffin using a authomatic Leica ASP300 machine. When observed, the > white matter of the spinal cord is full of holes and myelin seems to > be absent. > I guess the problem was related to the processing steps=erformed by > the machine, in particular the Xylol step. > Is there someone who can help me sharing his/her experience on spinal > cord process=ng protocol? > Any suggestion will be highly appreciated. > Regards > -- > Dott.ssa Elisa Ballarini,PhD student > Dipartimento di Neuroscie=ze e Tecnologie Biomediche > Universit? degli Studi Milano-Bicocca > Via C=dore 48-20052,Monza,MB > e.ballarini1@campus.unimib.it > Tel. 02-64488119 > Fax. 02-64488253 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Mon Mar 15 10:14:42 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Mar 15 10:14:51 2010 Subject: [Histonet] tb-controls selfmade Message-ID: <0E913F4199A24822A224135083751A49@dielangs.at> Hi all! Can someone provide me with a procedure for selfmade Tb-control for ZN-staining. I tried incubating some lungtissue (2x2x2 cm) in a solution with 3-6 drops of bacteria-suspension for 3 hours and for 18 hours. Afterwards fixing in NBF for 4 days. I had no success. No stainable mycobacteria were found. Bye Gudrun From lpjones <@t> srhs-pa.org Mon Mar 15 10:26:04 2010 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Mon Mar 15 10:26:09 2010 Subject: [Histonet] Block patient IDs In-Reply-To: Message-ID: <4AE8039AEA096143B965CBC6D0921668022429DD02@EXCH2007.srhs-pa.org> We are hand writing the LIS generated number on the front of each cassette and the patient's last name on the side. We have been doing this since the JCAHO rule came out. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Anne van Binsbergen Sent: Friday, March 12, 2010 10:42 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Block patient IDs A question for all you CAP fundis: how many patient Identifiers are needed on each paraffin block We currently just write the unique, LIS generated number on the block face, but have recently been advised that CAP and JCIA require not one, but TWO patient IDS on the block Comments please -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beth.Fye <@t> HCAhealthcare.com Mon Mar 15 11:09:38 2010 From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com) Date: Mon Mar 15 11:09:44 2010 Subject: [Histonet] Block patient IDs Message-ID: <938F8EC5A524D34EB5796E23E52781D329A89D6B21@NADCWPMSGCMS05.hca.corpad.net> Sometimes it is best to make changes in procedures not just because a regulatory agency requires it, but because it makes sense. We have been using 2 patient identifiers on our blocks for many years. I remember when I implemented it; I got a lot of flack from the staff about how cumbersome it would be. We still hand label all our cassettes and we do not have low volume. If you asked my staff now, they would unanimously agree that it is a good requirement and has saved us on many occasions with mislabeled cassettes. If the number is incorrect the name is almost always correct and vise versa. I agree that it is only a matter of time before the regulatory agencies require it. Why wait until it is required? If it improves patient safety, implement it now. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 From mpence <@t> grhs.net Mon Mar 15 11:14:16 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Mar 15 11:14:21 2010 Subject: [Histonet] Block patient IDs In-Reply-To: <938F8EC5A524D34EB5796E23E52781D329A89D6B21@NADCWPMSGCMS05.hca.corpad.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D90@is-e2k3.grhs.net> I have to ask how you dispose of your blocks since there is now a patient name on then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Monday, March 15, 2010 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block patient IDs Sometimes it is best to make changes in procedures not just because a regulatory agency requires it, but because it makes sense. We have been using 2 patient identifiers on our blocks for many years. I remember when I implemented it; I got a lot of flack from the staff about how cumbersome it would be. We still hand label all our cassettes and we do not have low volume. If you asked my staff now, they would unanimously agree that it is a good requirement and has saved us on many occasions with mislabeled cassettes. If the number is incorrect the name is almost always correct and vise versa. I agree that it is only a matter of time before the regulatory agencies require it. Why wait until it is required? If it improves patient safety, implement it now. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arvidsonkristen <@t> yahoo.com Mon Mar 15 12:06:04 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Mon Mar 15 12:06:16 2010 Subject: [Histonet] Contamination on Processor Message-ID: <276493.61883.qm@web65705.mail.ac4.yahoo.com> We are experiencing cloudiness in all of our alcohols on our processor.? We test them before we dump them by mixing them with water and they cloud up.? To me this says there is some clear-rite/xylene (we use both on our processors) getting in them somehow.? I was wondering if anyone else has experienced this or if there is a possibility it could be some other contaminant or is the cloudiness normal after so many processing runs.? We have newer processors, leica asp300s.? Any help would be so much appreciated.? thank you. From bhewlett <@t> cogeco.ca Mon Mar 15 12:21:46 2010 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Mar 15 12:21:55 2010 Subject: [Histonet] Contamination on Processor References: <276493.61883.qm@web65705.mail.ac4.yahoo.com> Message-ID: <0DF1DB440E18495BA28C6CC31F0F67C3@mainbox> Kristen, It's more likely due to the presence of lipids extracted from the tissues. How often do you replenish/change alcohols? Bryan ----- Original Message ----- From: "kristen arvidson" To: "histonet" Sent: Monday, March 15, 2010 1:06 PM Subject: [Histonet] Contamination on Processor We are experiencing cloudiness in all of our alcohols on our processor. We test them before we dump them by mixing them with water and they cloud up. To me this says there is some clear-rite/xylene (we use both on our processors) getting in them somehow. I was wondering if anyone else has experienced this or if there is a possibility it could be some other contaminant or is the cloudiness normal after so many processing runs. We have newer processors, leica asp300s. Any help would be so much appreciated. thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Mar 15 12:34:02 2010 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Mar 15 12:34:10 2010 Subject: [Histonet] Contamination on Processor In-Reply-To: <276493.61883.qm@web65705.mail.ac4.yahoo.com> References: <276493.61883.qm@web65705.mail.ac4.yahoo.com> Message-ID: <447184.19060.qm@web1112.biz.mail.sk1.yahoo.com> Are you mixing NBF and zinc formalin fixed tissue on the same processor? The phosphates in NBF will cause a zinc oxide?precipitate. Paula Pierce, BA, HTL(ASCP)HT President Excalibur Pathology 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: kristen arvidson To: histonet Sent: Mon, March 15, 2010 12:06:04 PM Subject: [Histonet] Contamination on Processor We are experiencing cloudiness in all of our alcohols on our processor.? We test them before we dump them by mixing them with water and they cloud up.? To me this says there is some clear-rite/xylene (we use both on our processors) getting in them somehow.? I was wondering if anyone else has experienced this or if there is a possibility it could be some other contaminant or is the cloudiness normal after so many processing runs.? We have newer processors, leica asp300s.? Any help would be so much appreciated.? thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jennifer.Johnson <@t> genzyme.com Mon Mar 15 13:38:20 2010 From: Jennifer.Johnson <@t> genzyme.com (Johnson, Jennifer(Hist)) Date: Mon Mar 15 13:39:04 2010 Subject: [Histonet] Processing lavages into cell pellets... Message-ID: <361F951D4CE3524383AD9D6F3D400939024C80E9@MAEXCH6.genzyme.com> Hi, I am looking for a little advice from anyone who has experience processing lavage fluid into cell pellets that can be sectioned and stained. I need to work out a protocol using lavages from knockout mice that may eventually be used to look at human patients. In both models of disease I expect to collect a lot of macrophages. After reading, "googling" and looking up articles and the Histonet, I have come up with the following "procedure". I would appreciate advice or ideas from anyone who has performed any part(s) of this type of procedure: 1. Collect lavage in PBS (perform actual lavage on euthanized mouse using PBS as the diluent). Store on ice until I get back to the lab. Question here - because I will be collecting many samples, should I spin them right away or keep them on ice and spin them all at once? Should I add a mucolytic agent (mucolexx or the like) and then store them on ice until I get back to my lab? I know that in mice, although it will be a model that has lung disease, mucus will not be a problem. However, in the human model of the disease it may be an issue. I need to see the effect of the mucolytic agent on the affected cells before using it on the patients' lavages. Does anyone out there use a specific mucolytic agent that they like? I plan on trying 2 - the Mucolexx (MSDS only lists formaldehyde) and Richard Allan Mucolytic agent (MSDS lists formaldehyde, ethylene glycol, methyl alcohol and polyethylene glycol). 2. Spin down cells at 4C for 15 minutes. Remove supernatant. Add fix - either overnight or for a few hours. Question - if I use a mucolytic agent that contains fixative - formaldehyde - do I have to rinse it out (resuspend the cells in PBS or other buffer and spin again) or can I just remove the sup and add a gel (step 4)? Is the mucolytic agent enough of a fixative (the Thermo/Richard Allan and Mucolexx mucolytic agents contain 0.3 or 3 % formaldehyde)? 3. Once the cells are pelleted, should I fix them again or just resuspend them in a gel (something like Histogel or an agar(ose)? Does anyone out there use either of these gels to look at cells? Any preferences? 4. Once the cells are in the gel, fix the cell pellet. (Is this necessary?) 5. Pray and process the pellet into paraffin (and maybe plastic too). Thanks in advance for any help you can give. Jenn Jennifer Johnson Genzyme Corp. Department of Pathology 5 Mountain Road Framingham, MA 01701-9322 Phn - 508-271-3610 Fax - 508-872-9080 From jesus.w.hdz <@t> gmail.com Mon Mar 15 15:19:59 2010 From: jesus.w.hdz <@t> gmail.com (Jesus Hernandez) Date: Mon Mar 15 15:20:07 2010 Subject: [Histonet] Staining hydroxyapatite scaffolds Message-ID: <8895cf801003151319u35118157t23e9c67250846e69@mail.gmail.com> Yea I wanted to know if anyone else has dealt with staining hydroxyapatite bone substitute scaffolds that have been placed inside rabbits. I have been having issues with the staining. I am not sure if the sample thickness has anything to do with why I keep getting blotches of dark staining. It appears to only be a problem with the paragon stain. I wanted to know if anyone knows of maybe a different type of staining that I should use to help avoid getting these blotches. I feel that the scaffold material is causing this issue so it might ultimately be unavoidable, but the reason I ask for a way to make my slides looks cleaner is because I do histomorphometry on the samples. The only two stains that I use are Paragon & Alazarin Red. I need to have tissue and bone distinguishable for my analysis. The scaffold usually absorbs both colors making it a dark black image on the microscope. Any pointers or suggestions would be greatly appreciated. Thanks, JH From JMitchell <@t> uwhealth.org Mon Mar 15 18:01:53 2010 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Mon Mar 15 18:01:58 2010 Subject: [Histonet] 2010 Tri-State Symposium Message-ID: <2108AECB05DBFF48A9C436A79215574002D2E511@UWHC-MAIL03.uwhis.hosp.wisc.edu> Dear Histonetters: You are invited to join the histology societies of Iowa, Minnesota and Wisconsin as they host the 2010 Tri-State Symposium, April 28-30, at The West Des Moines Marriott Hotel, West Des Moines, Iowa. For program & registration information visit our websites or contact the following representatives: Iowa: Judi Stasko (judith.stasko@ars.usda.gov) Minnesota: www.mnshonline.com Lois Rowe (rowe.lois@mayo.edu) Wisconsin: www.wistology.org Kathy Ellefson (kmellefson@gmail.com) From Tony_Reilly <@t> health.qld.gov.au Mon Mar 15 18:21:12 2010 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Mon Mar 15 18:24:49 2010 Subject: [Histonet] Processing lavages into cell pellets... In-Reply-To: <361F951D4CE3524383AD9D6F3D400939024C80E9@MAEXCH6.genzyme.com> References: <361F951D4CE3524383AD9D6F3D400939024C80E9@MAEXCH6.genzyme.com> Message-ID: <4B9F4D87.471C.0039.0@health.qld.gov.au> Hi Jennifer I realise there are products like Histogel about of which I have no knowledge however I have been using agar for over 30 years because I can get it easily and gratis from our microbiology department. My experience is to spin down the cells, remove the supernatent, then add fixative. The fixation helps to protect the cells from the heat of the molten agar. Heat agar until just melted to ensure minimum temperature. After adding molten agar spin again in a tube to give you a button of agar with the cells located at the pointy end. After the agar has set the agar button can be removed from the tube and stored in fixative for processing. Although there may be a small number of cells in the agar, the agar does not process well on a short cycle(ie 2 hours) Process on at least a 6 hour cycle and overnight is OK. When embedding, the cells if in sufficient quantity, are visible in the agar allowing easy orientation. All the best. regards Tony Tony Reilly Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Johnson, Jennifer(Hist)" 16/03/2010 4:38 am >>> Hi, I am looking for a little advice from anyone who has experience processing lavage fluid into cell pellets that can be sectioned and stained. I need to work out a protocol using lavages from knockout mice that may eventually be used to look at human patients. In both models of disease I expect to collect a lot of macrophages. After reading, "googling" and looking up articles and the Histonet, I have come up with the following "procedure". I would appreciate advice or ideas from anyone who has performed any part(s) of this type of procedure: 1. Collect lavage in PBS (perform actual lavage on euthanized mouse using PBS as the diluent). Store on ice until I get back to the lab. Question here - because I will be collecting many samples, should I spin them right away or keep them on ice and spin them all at once? Should I add a mucolytic agent (mucolexx or the like) and then store them on ice until I get back to my lab? I know that in mice, although it will be a model that has lung disease, mucus will not be a problem. However, in the human model of the disease it may be an issue. I need to see the effect of the mucolytic agent on the affected cells before using it on the patients' lavages. Does anyone out there use a specific mucolytic agent that they like? I plan on trying 2 - the Mucolexx (MSDS only lists formaldehyde) and Richard Allan Mucolytic agent (MSDS lists formaldehyde, ethylene glycol, methyl alcohol and polyethylene glycol). 2. Spin down cells at 4C for 15 minutes. Remove supernatant. Add fix - either overnight or for a few hours. Question - if I use a mucolytic agent that contains fixative - formaldehyde - do I have to rinse it out (resuspend the cells in PBS or other buffer and spin again) or can I just remove the sup and add a gel (step 4)? Is the mucolytic agent enough of a fixative (the Thermo/Richard Allan and Mucolexx mucolytic agents contain 0.3 or 3 % formaldehyde)? 3. Once the cells are pelleted, should I fix them again or just resuspend them in a gel (something like Histogel or an agar(ose)? Does anyone out there use either of these gels to look at cells? Any preferences? 4. Once the cells are in the gel, fix the cell pellet. (Is this necessary?) 5. Pray and process the pellet into paraffin (and maybe plastic too). Thanks in advance for any help you can give. Jenn Jennifer Johnson Genzyme Corp. Department of Pathology 5 Mountain Road Framingham, MA 01701-9322 Phn - 508-271-3610 Fax - 508-872-9080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From Tony_Reilly <@t> health.qld.gov.au Mon Mar 15 18:32:29 2010 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Mon Mar 15 18:34:35 2010 Subject: [Histonet] tb-controls selfmade In-Reply-To: <0E913F4199A24822A224135083751A49@dielangs.at> References: <0E913F4199A24822A224135083751A49@dielangs.at> Message-ID: <4B9F502C.471C.0039.0@health.qld.gov.au> Hi Gudren I have not done this with TB but I have with other bacteria to produce controls for gram stains. You need to have the tissue in a nutrient broth appropriate for the organism. You need to consult with a laboratory that specialises in Mycobacteria to find the appropriate medium to use. Also what may be relevant is that in the laboratory it takes up to six weeks to culture TB. Not sure but this may be why you are not getting results. regards Tony Tony Reilly Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Gudrun Lang" 16/03/2010 1:14 am >>> Hi all! Can someone provide me with a procedure for selfmade Tb-control for ZN-staining. I tried incubating some lungtissue (2x2x2 cm) in a solution with 3-6 drops of bacteria-suspension for 3 hours and for 18 hours. Afterwards fixing in NBF for 4 days. I had no success. No stainable mycobacteria were found. Bye Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From cscampbe <@t> uci.edu Mon Mar 15 18:42:16 2010 From: cscampbe <@t> uci.edu (cscampbe@uci.edu) Date: Mon Mar 15 18:42:20 2010 Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's solution Message-ID: <159c5f14a0ef0d1157eeec9b72f8bbc7.squirrel@webmail.uci.edu> Hi Histonet, After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it very difficult to remove the yellow coloring. Rinses with water are not doing the trick. Does anyone have some advice on how to bring the slides back to a clear color so that I may proceed with the Masson Trichrome procedure? Thanks! -Colin From AnthonyH <@t> chw.edu.au Mon Mar 15 19:03:00 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Mar 15 19:03:14 2010 Subject: [Histonet] Removing Yellow color from slides refixed in Bouin'ssolution In-Reply-To: <159c5f14a0ef0d1157eeec9b72f8bbc7.squirrel@webmail.uci.edu> Message-ID: Place slides in saturated lithium carbonate for a few minutes. This should do the trick Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cscampbe@uci.edu Sent: Tuesday, 16 March 2010 10:42 AM To: HistoNet Subject: [Histonet] Removing Yellow color from slides refixed in Bouin'ssolution Hi Histonet, After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it very difficult to remove the yellow coloring. Rinses with water are not doing the trick. Does anyone have some advice on how to bring the slides back to a clear color so that I may proceed with the Masson Trichrome procedure? Thanks! -Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tissuearray <@t> hotmail.com Mon Mar 15 19:27:22 2010 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Mon Mar 15 19:27:26 2010 Subject: [Histonet] Histo Stories Message-ID: I remember bring my 6 year old son to work with me one afternoon. I was on call and had to go in for a heart bx. He intently watch me cut and lay the sections on the water bath neatly. After about 10 minutes of watching me he said. "Do fighter pilots get paid? I said, "Yes." He said, "Oh good." he walked off to check out something else... Now he's in college to be a helicopter pilot. We still joke about it. _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_1 From Amanda.Woolsey <@t> imail.org Mon Mar 15 22:27:26 2010 From: Amanda.Woolsey <@t> imail.org (Amanda Woolsey) Date: Mon Mar 15 22:27:32 2010 Subject: [Histonet] JOH Article Question Message-ID: <6469E1E3C760EC4D95943272D28A694E75A0B4425C@LP-EXMBVS09.CO.IHC.COM> To Whom It May Concern, I am trying to contact Gayle Callis about the article she refers to in the text below. I would appreciate any information as I am trying to obtain a copy of this article. (I have already tried through the NSH website and I am just trying all of the avenues!) Thank you, Amanda Woolsey, HTL Histology - Pathology Primary Children's Medical Center Salt Lake City, Utah [Histonet] growing gram pos and neg Gayle Callis gcallis <@t> montana.edu Mon Aug 20 10:30:38 CDT 2007 * Previous message: [Histonet] growing gram pos and neg * Next message: [Histonet] Safran du Gatinais * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] Jennifer, There is a wonderful publication on making a combined Gram negative and Gram positive control in J of Histotechnology, December 2006. The author is Kristine J. Vaughn (do not have email contact here) but I will CC to my home email so I can put you in contact with her. She has reprints available, and will be delighted to send you one. Her method is simple and very effective, plus addresses biosafety issues with human tissue use. If you are a member of NSH, you can contact JOH and request this reprint free of charge too. Go to the NSH website, then click on JOH for this request. At 04:57 PM 8/17/2007, you wrote: >Hello all, > >I would like to hear your ideas on the best way to grow gram positive >and negative organisms in tissue. It would have to be tissue that is not >fixed so, what do you use? Did you grow the organisms, inject into >tissue and then put into incubator? or do you grow organisms on agar and >place tissue on it and incubate? or in a broth? Thank you for your >input. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Amanda.Woolsey <@t> imail.org Mon Mar 15 22:35:27 2010 From: Amanda.Woolsey <@t> imail.org (Amanda Woolsey) Date: Mon Mar 15 22:35:33 2010 Subject: [Histonet] Question: Homegrown Controls Message-ID: <6469E1E3C760EC4D95943272D28A694E75A0B4425D@LP-EXMBVS09.CO.IHC.COM> To Whom It May Concern, I am trying to contact Peggy Wenk in regards to the text below. I would really like to ask a few questions about the procedure outlined. Thank you, Amanda Woolsey, HTL Histology - Pathology Primary Children's Medical Center Salt Lake City, Utah [Histonet] AFB Control Lee & Peggy Wenk lpwenk <@t> covad.net Sat Mar 6 14:10:00 CST 2004 * Previous message: [Histonet] AFB Control * Next message: [Histonet] TUNEL staining in whole mount chick embryos * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] Someone asked a similar question about 2 weeks ago, but about gram controls. I included in my response that the technique also works for AFB, fungus and spirochetes. I'm therefore reprinting my response from 2 weeks ago: = = = = = = = = = = Start with fresh, unfixed placenta or lung. - If lung, slightly edematous works best. - If placenta, press between towels several times, to try to get the excess RBCs out. Gross tissue into 2-3 mm cubes. Contact microbiology to let them know you are on your way (after previously talking with the supervisor days earlier about your needs). Have the microbiology prepare tubes of liquid incubating media (appropriate for the type(s) of micro-organism(s) you need). - Gram negative (E. coli works well) - Gram positive - AFB (non-pathogenic) - Fungus - Spirochetes (rarely is it ever syphilis. Usually some type of large spirochete, unfortunately. When large spirochetes are positive with the silver stains, the small syphilis are not being demonstrated yet.) (Helicobacter, as far as I know, cannot be grown in incubating media in routine microbiology labs.) Incubate tissue in culture media in 37 degree C. incubator overnight. Pour 10% NBF in tubes in morning, and allow to fix for 30-60 minutes. Pour out formalin/incubating media mixture, and pour in fresh NBF. Allow to fix all day. Place tissues in cassettes, label, process as usual. Embed. Write up cost containment report, on how you make X number of blocks of control tissues, which would equal Y number of slides. Which, if you had to buy them from an outside source would have cost you $Z amount of money. And the cost for you to do this procedures was a little bit of tech time, a few cassettes and some paraffin. Therefore, you just saved your facility lots of money! Document the collaboration between Histology and Microbiology. Everyone looks like a winner. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From rmoody <@t> ameripath.com Tue Mar 16 00:28:40 2010 From: rmoody <@t> ameripath.com (Moody, Robert) Date: Tue Mar 16 00:28:51 2010 Subject: [Histonet] newyork exam for HT Message-ID: Hi,histonetters does any one know the website to apply for new york histology license I would like to obtain. Robert Moody AmeriPath Inc | Histolotechnician, IT | 1661 E. Camelback Rd., Suite 140 | Phoenix, AZ 85016 USA | phone +1.602.441.2035 | fax +1.602.441.2034 | Rmoody@AmeriPath.com | www.AmeriPath.com | P Please think about resource conservation before you print this message CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jkiernan <@t> uwo.ca Tue Mar 16 01:37:47 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Mar 16 01:37:50 2010 Subject: [Histonet] Question about new Frieda Carson book Message-ID: Go to a library and see if the book has the information you need. Decide whether to photocopy a few pages (for a specific job) or ask your lab to buy a copy of the book as a resource for all who work there. Every lab needs several books, and provision of time for the workers to read them. Carson's Histotechnology is a first-rate text, notable for giving the rationales of staining methods, practical instructions, photomicrographs of well stained examples and references for the readers who want to know more. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Valerie Rodriguez Date: Sunday, March 14, 2010 9:01 Subject: [Histonet] Question about new Frieda Carson book To: histonet@lists.utsouthwestern.edu > I have a question about the new Frieda Carson book: > Histotechnology: A > Self-Instructional Text 3rd edition. In this book there is a new > chapterabout cytopreparation techniques. I will like to know if > this chapter > teaches you how to troubleshoot problems in the staining of the > slides, how > to do fine needle aspirations and other techniques in cytology, > or it just > gives you a brief review of how to prepare cytology?????. I have the > previous version, the new book came right after I graduate > histotech school. > Is it worth to buy the new book? Someone told me the pictures > and text of > all the other chapters have not changed, just the new cytology > chapter. > My lab is concentrated more in fine needle aspirations > (cytology) than > histology so I think it will be good for me to buy the new > edition since is > like a chapter made just for histotech so that they can > undersand cytology > better, but since is new is a little pricey. I may wait until > the price is a > little lower. Frieda's book is very excelent in preparing > histotechs on how > to troubleshoot problems, but I will like to find a book like > that but for > cytotechnology. > > What are your opinions???? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Claire.Fahy <@t> agriculture.gov.ie Tue Mar 16 05:16:38 2010 From: Claire.Fahy <@t> agriculture.gov.ie (Fahy, Claire) Date: Tue Mar 16 05:16:50 2010 Subject: [Histonet] Superfrost Plus Slides Message-ID: <70E46C293A3B9647ACDBA7D5DAC0ADC0694C80@SKEBNEXCH01.agriculture.gov.ie> Hi I know this is in reply to an old threat but we are experiencing varying and uneven staining of our sections on the Ventana Benchmark.It is not confined to a perticular anitbody and doesn't happen all the time either. We have been told that it may be the slides.We currently use Thermo Scientific (Menzel-Glaser) superfrost plus slides.Does anyone use the Ventana IHC slides? Has anyone has experienced similar problems?If so,what have you done to resolve them. Thanks Claire fahy Lab analyst, Histopatholgy Lab Department of Agriculture, Fisheries and Food The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). An Roinn Talmha?ochta, Iascaigh agus Bia T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh ?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air, n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar ? do r?omhaire le do thoil. From Claire.Fahy <@t> agriculture.gov.ie Tue Mar 16 06:05:06 2010 From: Claire.Fahy <@t> agriculture.gov.ie (Fahy, Claire) Date: Tue Mar 16 06:05:21 2010 Subject: [Histonet] Superfrost Plus Slides In-Reply-To: Message-ID: <70E46C293A3B9647ACDBA7D5DAC0ADC0694C84@SKEBNEXCH01.agriculture.gov.ie> Hi Lynn Yes, our instrument is decontaminated and cleaned as per manual. There was some old threads on Histonet where some people had got varying staining between runs so I was wondering if anybody had found a solution to this problem.Maybe it is simply the slides? -----Original Message----- From: Lynn Lee [mailto:lynnlee2010@live.com] Sent: 16 March 2010 10:43 To: Fahy, Claire Subject: RE: [Histonet] Superfrost Plus Slides You could have contamination in the instrument. Has it been decontaminated quarterly per the instruction manual? I have never heard of uneven staining from plus slides. > Date: Tue, 16 Mar 2010 10:16:38 +0000 > From: Claire.Fahy@agriculture.gov.ie > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Superfrost Plus Slides > > Hi > > I know this is in reply to an old threat but we are experiencing varying and uneven staining of our sections on the Ventana Benchmark.It is not confined to a perticular anitbody and doesn't happen all the time either. > > We have been told that it may be the slides.We currently use Thermo Scientific (Menzel-Glaser) superfrost plus slides.Does anyone use the Ventana IHC slides? > > Has anyone has experienced similar problems?If so,what have you done to resolve them. > > Thanks > > Claire fahy > Lab analyst, > Histopatholgy Lab > > Department of Agriculture, Fisheries and Food > > The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). > > An Roinn Talmha?ochta, Iascaigh agus Bia > > T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh ?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air, n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar ? do r?omhaire le do thoil. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. Learn More. ________________________________ Department of Agriculture, Fisheries and Food The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). An Roinn Talmha?ochta, Iascaigh agus Bia T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh ?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air, n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar ? do r?omhaire le do thoil. From jl <@t> merraine.com Tue Mar 16 08:44:32 2010 From: jl <@t> merraine.com (Jonah Levinger) Date: Tue Mar 16 08:45:51 2010 Subject: [Histonet] Histology Management Openings, Northeast Message-ID: To fellow histoneters: Our recruitment firm, Merraine Group Inc., specializes in Laboratory and Pathology Administration. We have been retained on two exclusive Histology searches. Feel free to forward this information to your colleagues. Histology Supervisor: Large hospital in New York City. Two years of supervisory experience. Salary: $75,000 to 90,000 per year. Manager Anatomic Pathology: Large hospital in Maryland/Virginia region. Four years management experience. Salary: $95-110k per year. Jonah Levinger, Team Leader Medical Recruitment Merraine Group Inc. (845) 357-3355 Ex. 108 jl@merraine.com www.merraine.com https://www.logoworks.com/uploads/projects/183596601953/finalartwork/183596_ logo_final.jpg http://www.logoworks.com/app/client/LogoworksEmailTemplate/vr_2x58.gif Jonah Levinger Team Leader, Medical Recruitment Wk:845.357.3355x108 Main:845.290.1900 Fax: 212.918.9184 jl@merraine.com www.merraine.com From mcauliff <@t> umdnj.edu Tue Mar 16 09:13:48 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Mar 16 09:11:33 2010 Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's solution In-Reply-To: <159c5f14a0ef0d1157eeec9b72f8bbc7.squirrel@webmail.uci.edu> References: <159c5f14a0ef0d1157eeec9b72f8bbc7.squirrel@webmail.uci.edu> Message-ID: <4B9F921C.60308@umdnj.edu> A few drops of saturated aqueous lithium carbonate added to the 70% alcohol step will do the job nicely. Geoff cscampbe@uci.edu wrote: > Hi Histonet, > > After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it > very difficult to remove the yellow coloring. Rinses with water are not > doing the trick. Does anyone have some advice on how to bring the slides > back to a clear color so that I may proceed with the Masson Trichrome > procedure? > > Thanks! > -Colin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From vygutz <@t> yahoo.com Tue Mar 16 09:21:02 2010 From: vygutz <@t> yahoo.com (Vanessa Gutierrez) Date: Tue Mar 16 09:21:05 2010 Subject: [Histonet] (no subject) Message-ID: <394695.49021.qm@web30207.mail.mud.yahoo.com> UNSUBSCRIBE ME?FOR THE 5TH TIME!!!?I HAVE FOLLOWED INSTRUCTIONS AND STILL AM ON THIS LIST!?SORRY, I KNOW CAPS IS NOT ALLOWED SO?LETS SEE IF BREAKING THE RULES GETS ME OFF! THANK YOU From NSEARCY <@t> swmail.sw.org Tue Mar 16 09:27:51 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Tue Mar 16 09:28:07 2010 Subject: [Histonet] Inquiry Message-ID: <4B9F4F17.5D38.00EF.0@swmail.sw.org> Anyone know of a company that performs microarrays commercially? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From dimitri.scholz <@t> ucd.ie Tue Mar 16 09:49:05 2010 From: dimitri.scholz <@t> ucd.ie (Dimitri Scholz) Date: Tue Mar 16 09:48:49 2010 Subject: [Histonet] (no subject) In-Reply-To: <394695.49021.qm@web30207.mail.mud.yahoo.com> References: <394695.49021.qm@web30207.mail.mud.yahoo.com> Message-ID: <013701cac517$d339ca20$79ad5e60$%scholz@ucd.ie> Me too! Dimitri Scholz, PhD, Dr. Sci. Director of Biological Imaging Conway Institute University College Dublin (UCD) Belfield, Dublin 4 Ireland Tel: +353-1 716 6736 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Gutierrez Sent: 16 March 2010 14:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) UNSUBSCRIBE ME?FOR THE 5TH TIME!!!?I HAVE FOLLOWED INSTRUCTIONS AND STILL AM ON THIS LIST!?SORRY, I KNOW CAPS IS NOT ALLOWED SO?LETS SEE IF BREAKING THE RULES GETS ME OFF! THANK YOU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Tue Mar 16 09:49:11 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Mar 16 09:49:43 2010 Subject: [Histonet] How to unsubscribe In-Reply-To: <394695.49021.qm@web30207.mail.mud.yahoo.com> References: <394695.49021.qm@web30207.mail.mud.yahoo.com> Message-ID: <4B9F9A67.5030001@pathology.washington.edu> Vanessa, Did you click on this link at the bottom of every email? http://lists.utsouthwestern.edu/mailman/listinfo/histonet Once you get to the page scroll down until you see; To unsubscribe from Histonet, get a password reminder, or change your subscription options enter your subscription email address: Enter your email address and press unsubscribe. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 3/16/2010 7:21 AM, Vanessa Gutierrez wrote: > UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE RULES GETS ME OFF! > > THANK YOU > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Tue Mar 16 09:52:52 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Mar 16 09:52:57 2010 Subject: [Histonet] (no subject) In-Reply-To: <013701cac517$d339ca20$79ad5e60$%scholz@ucd.ie> References: <394695.49021.qm@web30207.mail.mud.yahoo.com> <013701cac517$d339ca20$79ad5e60$%scholz@ucd.ie> Message-ID: both of you are not allowed to use the internet anymore. because you can't follow instructions. WE CAN'T UNSUBSCRIBE YOU. ONLY YOU CAN. A state-imposed metaphysic or religion should be opposed, if necessary at pistol-point. We must fight for variety if we fight at all. The uniform is as dull as a sculptured egg. --Lawrence Durrell, Consequential Data, "Balthazar" On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz wrote: > Me too! > > Dimitri Scholz, PhD, Dr. Sci. > Director of Biological Imaging > Conway Institute > University College Dublin (UCD) > Belfield, Dublin 4 > Ireland > Tel: +353-1 716 6736 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa > Gutierrez > Sent: 16 March 2010 14:21 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > UNSUBSCRIBE ME?FOR THE 5TH TIME!!!?I HAVE FOLLOWED INSTRUCTIONS AND STILL AM > ON THIS LIST!?SORRY, I KNOW CAPS IS NOT ALLOWED SO?LETS SEE IF BREAKING THE > RULES GETS ME OFF! > > THANK YOU > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cpyse <@t> x-celllab.com Tue Mar 16 09:52:20 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Mar 16 09:53:05 2010 Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's solution In-Reply-To: <159c5f14a0ef0d1157eeec9b72f8bbc7.squirrel@webmail.uci.edu> References: <159c5f14a0ef0d1157eeec9b72f8bbc7.squirrel@webmail.uci.edu> Message-ID: <000101cac518$47a5dde0$d6f199a0$@com> I just place the slides into 80% ETOH for 2 minutes. Cindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cscampbe@uci.edu Sent: Monday, March 15, 2010 7:42 PM To: HistoNet Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's solution Hi Histonet, After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it very difficult to remove the yellow coloring. Rinses with water are not doing the trick. Does anyone have some advice on how to bring the slides back to a clear color so that I may proceed with the Masson Trichrome procedure? Thanks! -Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 16 09:54:51 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 16 09:55:04 2010 Subject: [Histonet] JOH Article Question In-Reply-To: <6469E1E3C760EC4D95943272D28A694E75A0B4425C@LP-EXMBVS09.CO.IHC.COM> Message-ID: <901572.24637.qm@web65713.mail.ac4.yahoo.com> If you are a member of the NSH you can ask them for a "pdf" of this, or any other article published in the JOH. Ren? J. --- On Mon, 3/15/10, Amanda Woolsey wrote: From: Amanda Woolsey Subject: [Histonet] JOH Article Question To: "histonet@lists.utsouthwestern.edu" Cc: "gcallis@montana.edu" Date: Monday, March 15, 2010, 11:27 PM To Whom It May Concern, I am trying to contact Gayle Callis about the article she refers to in the text below. I would appreciate any information as I am trying to obtain a copy of this article. (I have already tried through the NSH website and I am just trying all of the avenues!) Thank you, Amanda Woolsey, HTL Histology - Pathology Primary Children's Medical Center Salt Lake City, Utah [Histonet] growing gram pos and neg Gayle Callis gcallis <@t> montana.edu Mon Aug 20 10:30:38 CDT 2007 ? ? * Previous message: [Histonet] growing gram pos and neg ? ? * Next message: [Histonet] Safran du Gatinais ? ? * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] Jennifer, There is a wonderful publication on making a combined Gram negative and Gram positive control in J of Histotechnology, December 2006.???The author is Kristine J. Vaughn (do not have email contact here) but I will CC to my home email so I can put you in contact with her.? She has reprints available, and will be delighted to send you one.? Her method is simple and very effective, plus addresses biosafety issues with human tissue use.???If you are a member of NSH, you can contact JOH and request this reprint free of charge too.? Go to the NSH website, then click on JOH for this request. ? ???At 04:57 PM 8/17/2007, you wrote: >Hello all, > >I would like to hear your ideas on the best way to grow gram positive >and negative organisms in tissue. It would have to be tissue that is not >fixed so, what do you use? Did you grow the organisms, inject into >tissue and then put into incubator? or do you grow organisms on agar and >place tissue on it and incubate? or in a broth? Thank you for your >input. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From br <@t> merraine.com Tue Mar 16 09:57:24 2010 From: br <@t> merraine.com (Barbara Ratner) Date: Tue Mar 16 09:58:41 2010 Subject: [Histonet] Unsubscribe please Message-ID: Barbara Ratner Vice President Merraine Group Inc. One Executive Blvd. Suite #110 Montebello, NY 10901 direct: (845) 290-1900 main: (845) 357-3355 x104 fax (212) 918-9184 e-mail: br@merraine.com or br@sensationaltalent.com www.merraine.com "Providing Leaders for Healthcare & Energy" Domestic & International Placements From talulahgosh <@t> gmail.com Tue Mar 16 10:08:00 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Mar 16 10:08:05 2010 Subject: [Histonet] Unsubscribe please In-Reply-To: References: Message-ID: JUPITER'S THUNDER!!!!!!!!!!!!! GO TO THIS WEBSITE. http://lists.utsouthwestern.edu/mailman/listinfo/histonet SCROLL DOWN TO THE BOX WHERE IT SAYS UNSUBSCRIBE (MAKE SURE YOU USE THE CORRECT BOX OR YOU WILL NOT BE UNSUBSCRIBED) AND TYPE IN YOUR EMAIL. CLICK ON THE BOX THAT SAYS UNSUBSCRIBE. DO NOT WRITE TO THE LIST. A state-imposed metaphysic or religion should be opposed, if necessary at pistol-point. We must fight for variety if we fight at all. The uniform is as dull as a sculptured egg. --Lawrence Durrell, Consequential Data, "Balthazar" On Tue, Mar 16, 2010 at 10:57 AM, Barbara Ratner wrote: > > > Barbara Ratner > Vice President > Merraine Group Inc. > One Executive Blvd. > Suite #110 > Montebello, NY 10901 > > direct: (845) 290-1900 > main: (845) 357-3355 x104 > fax (212) 918-9184 > e-mail: br@merraine.com ?or br@sensationaltalent.com > www.merraine.com > > "Providing Leaders for Healthcare & Energy" > Domestic & International Placements > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From alyssa <@t> alliedsearchpartners.com Tue Mar 16 10:02:24 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Mar 16 10:08:57 2010 Subject: [Histonet] Question Salary Compensation Message-ID: Hello, I am not sure if this is too far off of subject here. However, I have a client in NYC, brand new private lab who is looking into hiring Sales Reps to aquire more clients to read slides for, within his Pathology Lab. Does anyone out there in histoland have an idea of a Sales Reps Salary working with Private Pathology Laboratory? Thank you in advance for any help. -Alyssa From lactose.intolerant <@t> yahoo.com Tue Mar 16 10:15:11 2010 From: lactose.intolerant <@t> yahoo.com (aa aa) Date: Tue Mar 16 10:15:15 2010 Subject: [Histonet] UNSUSCRIVE REQUIST Message-ID: <656052.73037.qm@web55308.mail.re4.yahoo.com> How Do I unsuscrive from this list please From anonwums1 <@t> gmail.com Tue Mar 16 10:27:28 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Tue Mar 16 10:27:34 2010 Subject: [Histonet] Unsubscribe please In-Reply-To: References: Message-ID: <858249121003160827q57608207s20527abc1fb7561c@mail.gmail.com> I think a lot of grief could be saved if the managers of HistoNet added the following four words at the bottom of each e-mail. Who would I need to contact to make such a suggestion? http://lists.utsouthwestern.edu/mailman/listinfo/histonet to change subscription preferences Adam On Tue, Mar 16, 2010 at 10:08 AM, Emily Sours wrote: > JUPITER'S THUNDER!!!!!!!!!!!!! > GO TO THIS WEBSITE. > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > SCROLL DOWN TO THE BOX WHERE IT SAYS UNSUBSCRIBE (MAKE SURE YOU USE > THE CORRECT BOX OR YOU WILL NOT BE UNSUBSCRIBED) AND TYPE IN YOUR > EMAIL. > CLICK ON THE BOX THAT SAYS UNSUBSCRIBE. > DO NOT WRITE TO THE LIST. > > A state-imposed metaphysic or religion should be opposed, if necessary > at pistol-point. We must fight for variety if we fight at all. The > uniform is as dull as a sculptured egg. > --Lawrence Durrell, Consequential Data, "Balthazar" > > > > On Tue, Mar 16, 2010 at 10:57 AM, Barbara Ratner wrote: > > > > > > Barbara Ratner > > Vice President > > Merraine Group Inc. > > One Executive Blvd. > > Suite #110 > > Montebello, NY 10901 > > > > direct: (845) 290-1900 > > main: (845) 357-3355 x104 > > fax (212) 918-9184 > > e-mail: br@merraine.com or br@sensationaltalent.com > > www.merraine.com > > > > "Providing Leaders for Healthcare & Energy" > > Domestic & International Placements > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mcauliff <@t> umdnj.edu Tue Mar 16 10:30:20 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Mar 16 10:28:04 2010 Subject: [Histonet] the spammer is back! In-Reply-To: <656052.73037.qm@web55308.mail.re4.yahoo.com> References: <656052.73037.qm@web55308.mail.re4.yahoo.com> Message-ID: <4B9FA40C.8030408@umdnj.edu> Dear all: aa aa is a troll/spammer. Do not reply to his attempts to cause trouble, it only encourages him. Geoff aa aa wrote: > How Do I unsuscrive from this list please > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From e.ballarini1 <@t> campus.unimib.it Tue Mar 16 10:33:54 2010 From: e.ballarini1 <@t> campus.unimib.it (Elisa Ballarini) Date: Tue Mar 16 10:34:01 2010 Subject: [Histonet] Fwd: spinal cord paraffin embedding Message-ID: Hi. First of all I would like to thank all of you for your advices. Because, my previous e-mail wasn't complete and to avoid further suggestions regarding protocols I can not follow, here I write some more information. First of all, we can't perfuse our rats. Also, when we harvest rat spinal cord (cut in half, thus around 3mm diameter) we fix it only for 4-5 hours in 4% paraformaldehyde because we need to perform an IHC essay so we would like to avoid epitope masking. We don't think fixation is the problem since frozen sections of the same fixed spinal cords do not show any problem in the white matter. In conclusion, we need to obtain good results using paraffin both in term Our paraffin embedding machine program is as follows: Ethanol 95% 1h Ethanol 95% 45' Ethanol 95% 45' Ethanol 100% 45' Ethanol 100% 1h Ethanol 100% 1h Ethanol 100% 1h Xylol 45' Xylol 1h Xylol 1h Paraffin 1h Paraffin 1h Paraffin 1h Can you tell me if are there any specific protocols to embedd rat spinal Thank you. Regards -- -- Dott.ssa Elisa Ballarini,PhD student Dipartimento di Neuroscie Universit? degli Studi Milano-Bicocca Via Cadore 48-20052,Monza,MB e.ballarini1@campus.uni Tel. 02-64488119 Fax. 02-64488253 --- segue il m From Allison_Scott <@t> hchd.tmc.edu Tue Mar 16 11:12:39 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Tue Mar 16 11:13:42 2010 Subject: [Histonet] Accessioning and Grossing Alike Specimens Message-ID: <1872B4A455B7974391609AD8034C79FC8BD710@LBEXCH01.hchd.local> What policies do you have in place for accessioning and grossing specimens that are alike, especiall biopsy specimens. It has been my experience that you should not have alike specimens back to back. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 5656 Kelley Houston, Texas 77026 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From vavalos <@t> allergydermatology.com Tue Mar 16 11:58:35 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Tue Mar 16 11:58:44 2010 Subject: FW: [Histonet] How to unsubscribe Message-ID: <000f01cac529$eb208b30$c161a190$@com> Oh yes, I have gone to the link, gone to the home page and followed all of the instructions for unsubscribing. Replied with the code it gives you, have been given a confirmation email and still I am flooded with this. Have done that twice and posted it three times now. I added my work email to this and want my personal one off. I enjoy reading about all the topics but not twice. Lol. Thanks anyway. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Tuesday, March 16, 2010 7:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How to unsubscribe Vanessa, Did you click on this link at the bottom of every email? http://lists.utsouthwestern.edu/mailman/listinfo/histonet Once you get to the page scroll down until you see; To unsubscribe from Histonet, get a password reminder, or change your subscription options enter your subscription email address: Enter your email address and press unsubscribe. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 3/16/2010 7:21 AM, Vanessa Gutierrez wrote: > UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE RULES GETS ME OFF! > > THANK YOU > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vavalos <@t> allergydermatology.com Tue Mar 16 12:01:22 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Tue Mar 16 12:01:31 2010 Subject: [Histonet] UNSUBSCRIBE Message-ID: <001001cac52a$4ef4ba00$ecde2e00$@com> I can only speak for myself but yes, I am aware of all steps to be taken in unsubscribing. Have followed them more than once but have been unsuccessful. So yes, my frustration as lead me to write IN ALL CAPS!!!!! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, March 16, 2010 7:53 AM To: Dimitri Scholz Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) both of you are not allowed to use the internet anymore. because you can't follow instructions. WE CAN'T UNSUBSCRIBE YOU. ONLY YOU CAN. A state-imposed metaphysic or religion should be opposed, if necessary at pistol-point. We must fight for variety if we fight at all. The uniform is as dull as a sculptured egg. --Lawrence Durrell, Consequential Data, "Balthazar" On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz wrote: > Me too! > > Dimitri Scholz, PhD, Dr. Sci. > Director of Biological Imaging > Conway Institute > University College Dublin (UCD) > Belfield, Dublin 4 > Ireland > Tel: +353-1 716 6736 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa > Gutierrez > Sent: 16 March 2010 14:21 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM > ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE > RULES GETS ME OFF! > > THANK YOU > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Tue Mar 16 12:40:23 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Mar 16 12:40:18 2010 Subject: [Histonet] Exciting Histotech Job in New England!! Message-ID: <1516952045.1268761223703.JavaMail.cfservice@webserver54> Hi Histotechs, I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great job opportunities in various areas across the country. One particular client that I am working with is a financially sound, medically advanced, full-service hospital in New England. My client is looking for both a HTL(ASCP) certified histotechnologist and a HT(ASCP) certified histotech for their hospital. Both positions would work a day shift. My client is offering a very competitive compensation package, full benefits, and relocation assistance. If interested, please email me a copy of your resume and follow-up with a call to learn more! Below is a list of some of the great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Opportunities: CT - Histology Operations Manager NY - New York City - Histotech 3rd shift NV -Las Vegas - Histotech 3rd shift NV Las Vegas - Histology Supervisor - 3rd shift PA - Cytology Supervisor CA - Palm Springs - Histotech - 1st shift CA - Southern - Histotech VA - Histotech 2nd shift NH - HTL and HT GA - Atlanta - Histotech GA - Atlanta - Pathology Coordinator (HT or CT) NV - Pathologist Assistant TN - Pathologist Assistant ND - Pathologist Assistant GA - Pathologist Assistant If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From Kim.Donadio <@t> bhcpns.org Tue Mar 16 12:47:31 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Mar 16 12:47:47 2010 Subject: [Histonet] UNSUBSCRIBE In-Reply-To: <001001cac52a$4ef4ba00$ecde2e00$@com> Message-ID: I have a suggestion. If you are extremely frustrated with getting all the emails that come from here and you are having a hard time unsubscribing, perhaps you could put a block on histonet so you cant receive emails from them until you get unsubscribed? Just a thought. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Vanessa Avalos" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/16/2010 12:01 PM To "'HISTONET LISTS'" cc Subject [Histonet] UNSUBSCRIBE I can only speak for myself but yes, I am aware of all steps to be taken in unsubscribing. Have followed them more than once but have been unsuccessful. So yes, my frustration as lead me to write IN ALL CAPS!!!!! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, March 16, 2010 7:53 AM To: Dimitri Scholz Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) both of you are not allowed to use the internet anymore. because you can't follow instructions. WE CAN'T UNSUBSCRIBE YOU. ONLY YOU CAN. A state-imposed metaphysic or religion should be opposed, if necessary at pistol-point. We must fight for variety if we fight at all. The uniform is as dull as a sculptured egg. --Lawrence Durrell, Consequential Data, "Balthazar" On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz wrote: > Me too! > > Dimitri Scholz, PhD, Dr. Sci. > Director of Biological Imaging > Conway Institute > University College Dublin (UCD) > Belfield, Dublin 4 > Ireland > Tel: +353-1 716 6736 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa > Gutierrez > Sent: 16 March 2010 14:21 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM > ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE > RULES GETS ME OFF! > > THANK YOU > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From kblack <@t> digestivehlth.com Tue Mar 16 12:59:16 2010 From: kblack <@t> digestivehlth.com (Konni Black) Date: Tue Mar 16 12:59:24 2010 Subject: [Histonet] HT/HTL position Gainesville. FL Message-ID: Fulltime HT/HTL opening in GI lab in Gainesville, Florida. Must have 3-5 years experience. Great lab. Flexible work schedule. Contact Konni Black at kblack@digestivehlth.com or 253-503-2560. From cbarone <@t> NEMOURS.ORG Tue Mar 16 13:10:50 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Tue Mar 16 13:10:56 2010 Subject: [Histonet] Re: proficiency testing for Muscle EHC- cont'd. Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7AFC@wlmmsx01.nemours.org> Histonetter's I only received one reply on my question regarding "what everyone is doing for proficiency testing of enzyme HC staining for muscle"...espeically for CLIA.compliance, now that I will lose my indepent reviewer. My thanks to Jan Minchew for her reply. I am working from that for now, though my sitiation somewhat different. .....but, I am finding it particularly frustrating finding an answer to this question over the last three months or more, that I have searched everywhere from the histonet to the internet toevery friend I have doing EHC. Is it required under CLIA? My auditor says yes..so I am trying to comply, though I cannot fiind it specifically in any CLIA reg. Who is the CLIA expert out there...and why is this not included in HQIP? Can someone shed more light. Thx. CB From sparker <@t> vt.edu Tue Mar 16 13:20:02 2010 From: sparker <@t> vt.edu (Scott Parker) Date: Tue Mar 16 13:22:55 2010 Subject: [Histonet] Spencer AO 820 microtome questions Message-ID: Dear all, I have access to a Spencer AO 820 microtome (a "black beauty" in Histo-parlance) that is does not have a disposable blade holder nor a chuck for holding paraffin cassettes. The microtome otherwise appears to be in good condition. I would like to get suggestions for where I might be able to purchase a chuck and disposable blade holder for this classic model. Alternatively, would it be a better idea for me to put this piece of equipment on display in a glass case and instead purchase a newer microtome. My work involves relatively low volume, basic sectioning of paraffin blocks for teaching and research. I don't need anything too fancy, just a microtome that is reliable and appropriate for student researchers to use. Thank you in advance for your responses. Scott Scott L. Parker, Ph.D. Assistant Professor Biology Coastal Carolina University P.O. Box 261954 Conway, SC 29528-6054 Office Phone: (843)-349-2491 From mturner <@t> carisdx.com Tue Mar 16 13:52:43 2010 From: mturner <@t> carisdx.com (Turner, Mark) Date: Tue Mar 16 13:52:49 2010 Subject: [Histonet] Job Openings in Phoenix, Arizona Message-ID: Hello Histonetters! Caris MPI, a division of Caris Life Sciences, is looking for 2 histotechs to assist with a dramatically increasing workflow. We are a very dynamic IHC lab which is growing exponentially. The positions are day shift, Monday through Friday, with occasional weekends. The successful candidates will have HT (ASCP) certification, 1-2 years IHC experience, preferably with the Ventana and Dako platforms, and will enjoy being part of a fast paced, quality oriented team. Qualification in Immunohistochemistry (QIHC) is a plus. Caris MPI is located in sunny Phoenix, AZ and will be moving to a brand new lab in May 2010. Equipment is state of the art, and there is a corporate commitment toward quality. Caris has had a presence in the Phoenix area for 3 years and is continuing to grow. The area is the 5th largest metropolitan in the country and has available anything you need to make life more interesting. If you would like to grow with us, please submit your resume in confidence to mturner@carisdx.com. Mark Turner, Ph. D., HT(ASCP) QIHC Supervisor IHC Target Now MPI Caris Life Sciences 445 N. 5th Street Phoenix, AZ 85004 Cell: 602-309-5084 Direct 602-358-8913 Fax: 602-358-8919 mturner@carisdx.com From mturner <@t> carisdx.com Tue Mar 16 13:57:47 2010 From: mturner <@t> carisdx.com (Turner, Mark) Date: Tue Mar 16 13:57:52 2010 Subject: [Histonet] Lab Assistant Position in Phoenix, AZ Message-ID: Caris MPI, a division of Caris Life Sciences, is looking to hire a Lab Assistant for 2nd shift. This will be a support position for our team of histology professionals, and is a great entry level position into the laboratory setting. Some college is preferred, with a focus on the sciences. Opportunities for advancement abound, as this position is open due to our previous lab assistant moving into a histotech role. Caris MPI is located in sunny Phoenix, AZ and will be moving to a brand new lab in May 2010. Equipment is state of the art, and there is a corporate commitment toward quality. Caris has had a presence in the Phoenix area for 3 years and is continuing to grow. The area is the 5th largest metropolitan in the country and has available anything you need to make life more interesting. If you would like to grow with us, please submit your resume in confidence to mturner@carisdx.com. Mark Turner, Ph. D., HT(ASCP) QIHC Supervisor IHC Target Now MPI Caris Life Sciences 445 N. 5th Street Phoenix, AZ 85004 Cell: 602-309-5084 Direct 602-358-8913 Fax: 602-358-8919 mturner@carisdx.com From sheila_adey <@t> hotmail.com Tue Mar 16 14:04:23 2010 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Mar 16 14:04:26 2010 Subject: [Histonet] Saffron/Eosin as a counterstain for H&E??? Message-ID: Hello, Our pathologist said that he say H&E slides with a counterstain that had some amount of saffron in it? Is anyone familiar with this? Sheila Adey HT MLT _________________________________________________________________ Stay in touch. http://go.microsoft.com/?linkid=9712959 From liz <@t> premierlab.com Tue Mar 16 14:30:38 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Mar 16 14:30:42 2010 Subject: [Histonet] Saffron/Eosin as a counterstain for H&E??? In-Reply-To: Message-ID: Its hematoxylin, ploxine and saffron. Believe it or not it was the stain that we used at my first job back in 1973. We also fixed in Zenkers too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Tuesday, March 16, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Saffron/Eosin as a counterstain for H&E??? Hello, Our pathologist said that he say H&E slides with a counterstain that had some amount of saffron in it? Is anyone familiar with this? Sheila Adey HT MLT _________________________________________________________________ Stay in touch. http://go.microsoft.com/?linkid=9712959_________________________________ ______________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathmaster <@t> yahoo.com Tue Mar 16 16:07:54 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Tue Mar 16 16:07:58 2010 Subject: [Histonet] NY License Message-ID: <12350.35531.qm@web111103.mail.gq1.yahoo.com> New York State Education Department- google it and you'll see a link for licensed professionals. Jeff Silverman From pathmaster <@t> yahoo.com Tue Mar 16 16:14:57 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Tue Mar 16 16:15:01 2010 Subject: [Histonet] (no subject) Message-ID: <161299.62124.qm@web111104.mail.gq1.yahoo.com> Don't know what you mean by rinses, but 5 to at most 10 minutes in gently running tap water should do the trick. If not, the lithium carbonate is what's prescribed. Jeff Silverman From cbrya <@t> lexclin.com Tue Mar 16 16:19:33 2010 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Tue Mar 16 16:19:38 2010 Subject: [Histonet] CAP cancer protocols and checklists Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF0567DDC388@EXCHANGESB> I am interested in how you are implementing the CAP cancer protocols in your laboratory? Are you using synoptic reporting, dictating from the checklists, etc? Any information you can provide would be greatly appreciated. Thank you in advance for your comments and input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From pathmaster <@t> yahoo.com Tue Mar 16 16:23:45 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Tue Mar 16 16:23:49 2010 Subject: [Histonet] (no subject) Message-ID: <190750.38557.qm@web111106.mail.gq1.yahoo.com> Hi Allison- That's our policy- don't accession like biopsies back to back. But if it is unavoidable, you can mark the second like biopsy case with a dab of yellow margin ink on at least one piece in each block and dictate that into the gross.? ie One fragment is marked in yellow for positive patient identification. Jeff Silverman- busy PA From saby_joseph_a <@t> yahoo.com Tue Mar 16 16:25:01 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Tue Mar 16 16:25:07 2010 Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's solution In-Reply-To: <000101cac518$47a5dde0$d6f199a0$@com> References: <159c5f14a0ef0d1157eeec9b72f8bbc7.squirrel@webmail.uci.edu> <000101cac518$47a5dde0$d6f199a0$@com> Message-ID: <619715.30232.qm@web113816.mail.gq1.yahoo.com> Running tap water for 10 minutes should do the trick. Joe Saby ________________________________ From: Cynthia Pyse To: cscampbe@uci.edu; HistoNet Sent: Tue, March 16, 2010 10:52:20 AM Subject: RE: [Histonet] Removing Yellow color from slides refixed in Bouin's solution I just place the slides into 80% ETOH for 2 minutes. Cindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cscampbe@uci.edu Sent: Monday, March 15, 2010 7:42 PM To: HistoNet Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's solution Hi Histonet, After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it very difficult to remove the yellow coloring. Rinses with water are not doing the trick. Does anyone have some advice on how to bring the slides back to a clear color so that I may proceed with the Masson Trichrome procedure? Thanks! -Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Tue Mar 16 16:34:15 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Mar 16 16:34:21 2010 Subject: [Histonet] (no subject) In-Reply-To: <161299.62124.qm@web111104.mail.gq1.yahoo.com> References: <161299.62124.qm@web111104.mail.gq1.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E240DD@PHSXMB30.partners.org> We also just wash in running tap water and the yellow stain disappears. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Tuesday, March 16, 2010 5:15 PM To: cscampbe@uci.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Don't know what you mean by rinses, but 5 to at most 10 minutes in gently running tap water should do the trick. If not, the lithium carbonate is what's prescribed. Jeff Silverman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From wdesalvo.cac <@t> hotmail.com Tue Mar 16 19:33:50 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Mar 16 19:33:58 2010 Subject: [Histonet] (no subject) In-Reply-To: <190750.38557.qm@web111106.mail.gq1.yahoo.com> References: <190750.38557.qm@web111106.mail.gq1.yahoo.com> Message-ID: An additional method to use to create positive identification when accessioning and grossing like specimens together when you must or just to increase the indetification factor for all specimens: Use the Davidson marking system and have the grossing person rotate the colors used to mark margins or small tissue by case and/or specimen and of course dictate to specific color used. Once they are in the habit, no two cases or specimens will have the same ink color and you increase quality assurance. William DeSalvo, B.S., HTL(ASCP) > Date: Tue, 16 Mar 2010 14:23:45 -0700 > From: pathmaster@yahoo.com > To: Allison_Scott@hchd.tmc.edu > CC: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Hi Allison- > That's our policy- don't accession like biopsies back to back. > But if it is unavoidable, you can mark the second like biopsy case with a dab of yellow margin ink on at least one piece in each block and dictate that into the gross. ie One fragment is marked in yellow for positive patient identification. > > Jeff Silverman- busy PA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850553/direct/01/ From mwfolsom <@t> rgbio.com Tue Mar 16 19:53:05 2010 From: mwfolsom <@t> rgbio.com (Michael Folsom) Date: Tue Mar 16 19:52:48 2010 Subject: [Histonet] Spencer AO 820 microtome questions In-Reply-To: References: Message-ID: <1268787185.6216.14.camel@chico.322tulane.org> Scott: Does the chuck have a clamp in it? If so soak a piece of soft wood that fits in the chuck in 100% ETOH (pulling a vacuum on it would help) and transition into paraffin. Once its soaked in paraffin pull it out and allow to solidify. Your tissue will need to be flat embedded in a simple boat. Use a razor blade to cut it out but leave a good bit of paraffin behind and around it. Once its cool use an artists pallet knife (small) to melt the surface of the paraffin on the wood and the back of the block and weld in place with the melted paraffin. Re: the knife - they make a knife holder that holds either hi or low profile disposable blades which fits in the microtome's knife holder. One thing about the old AO - they are almost indestructible but never back up the crank because it can strip the gears. Good luck - Mike mwfolsom@rgbio.cm On Tue, 2010-03-16 at 14:20 -0400, Scott Parker wrote: > Dear all, > > I have access to a Spencer AO 820 microtome (a "black beauty" in > Histo-parlance) that is does not have a disposable blade holder nor a chuck > for holding paraffin cassettes. The microtome otherwise appears to be in > good condition. I would like to get suggestions for where I might be able to > purchase a chuck and disposable blade holder for this classic model. > Alternatively, would it be a better idea for me to put this piece of > equipment on display in a glass case and instead purchase a newer microtome. > My work involves relatively low volume, basic sectioning of paraffin blocks > for teaching and research. I don't need anything too fancy, just a microtome > that is reliable and appropriate for student researchers to use. > > Thank you in advance for your responses. > > Scott > > > Scott L. Parker, Ph.D. > > Assistant Professor Biology > > Coastal Carolina University > > P.O. Box 261954 > > Conway, SC 29528-6054 > > > > Office Phone: (843)-349-2491 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Mar 16 22:05:55 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Mar 16 22:06:00 2010 Subject: [Histonet] Saffron/Eosin as a counterstain for H&E??? In-Reply-To: References: Message-ID: There is Garvey,W (1991): Modification of the Mayer hematoxylin stain. J. Histotechnol. 14, 163-165. The title is rather confusing. Her method included a modified composition of Mayer's haemalum (ethanol instead of chloral hydrate, and less alum), and instead of eosin Y the counterstain was a solution containing phloxin B (CI45410, Acid red 92) and saffron (which is CI 75100, Natural yellow 6). The author stated that saffron bought from a health food store was OK and much less expensive than that bought from a lab vendor. This counterstain was used by Rostoker et al (2001) Nephrology Dialysis Transplantation 16:513-517 in a study of intestinal pathology in glomerulonephritis. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: sheila adey Date: Tuesday, March 16, 2010 15:05 Subject: [Histonet] Saffron/Eosin as a counterstain for H&E??? To: histonet@lists.utsouthwestern.edu > > Hello, > > Our pathologist said that he say H&E slides with a counterstain > that had some amount of saffron in it? > > Is anyone familiar with this? > > Sheila Adey HT MLT > > > > _________________________________________________________________ > Stay in touch. > http://go.microsoft.com/?linkid=9712959_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfitz <@t> 007group.com Tue Mar 16 22:34:43 2010 From: cfitz <@t> 007group.com (Cathy) Date: Tue Mar 16 22:34:41 2010 Subject: [Histonet] Superfrost Plus Slides In-Reply-To: <70E46C293A3B9647ACDBA7D5DAC0ADC0694C84@SKEBNEXCH01.agriculture.gov.ie> References: <70E46C293A3B9647ACDBA7D5DAC0ADC0694C84@SKEBNEXCH01.agriculture.gov.ie> Message-ID: Hi Claire, We experienced problems with our IHC staining the fall of 2008 and it was traced back to a problem with particular lot numbers of the Superfrost slides. It was difficult to track down due to the inconsistency of the problem and the variation in the problem. We finally spoke with our Ventana technical specialist and she was able to tie our problem to problems that other facilities were experiencing. You could try contacting your Ventana technical resource person; they may be able to help. One thing we found out was that the Superfrost slides are manufactured at one site and distributed to vendors under their own name. Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fahy, Claire Sent: Tuesday, March 16, 2010 4:05 AM To: Lynn Lee Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Superfrost Plus Slides Hi Lynn Yes, our instrument is decontaminated and cleaned as per manual. There was some old threads on Histonet where some people had got varying staining between runs so I was wondering if anybody had found a solution to this problem.Maybe it is simply the slides? -----Original Message----- From: Lynn Lee [mailto:lynnlee2010@live.com] Sent: 16 March 2010 10:43 To: Fahy, Claire Subject: RE: [Histonet] Superfrost Plus Slides You could have contamination in the instrument. Has it been decontaminated quarterly per the instruction manual? I have never heard of uneven staining from plus slides. > Date: Tue, 16 Mar 2010 10:16:38 +0000 > From: Claire.Fahy@agriculture.gov.ie > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Superfrost Plus Slides > > Hi > > I know this is in reply to an old threat but we are experiencing varying and uneven staining of our sections on the Ventana Benchmark.It is not confined to a perticular anitbody and doesn't happen all the time either. > > We have been told that it may be the slides.We currently use Thermo Scientific (Menzel-Glaser) superfrost plus slides.Does anyone use the Ventana IHC slides? > > Has anyone has experienced similar problems?If so,what have you done to resolve them. > > Thanks > > Claire fahy > Lab analyst, > Histopatholgy Lab > > Department of Agriculture, Fisheries and Food > > The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). > > An Roinn Talmha?ochta, Iascaigh agus Bia > > T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh ?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air, n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar ? do r?omhaire le do thoil. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. Learn More. ________________________________ Department of Agriculture, Fisheries and Food The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). An Roinn Talmha?ochta, Iascaigh agus Bia T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh ?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air, n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar ? do r?omhaire le do thoil. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aazath <@t> hotmail.com Tue Mar 16 23:50:39 2010 From: aazath <@t> hotmail.com (Aazath Raj) Date: Wed Mar 17 00:23:18 2010 Subject: [Histonet] problem in IF stains In-Reply-To: References: Message-ID: Dear Friends, I am having a problem in IF stains on Renal Bx.In direct immunofloresence on the frozen sections does not have disered contrast and many of the cases its falsely negative. Can you people tell me the possible mistake which may cause these kind of effects on the IF stain. I dont see any gross mistakes on the Frozen sections after sectioning.All the temperature and other things are fine on the cryostate, but my pathologist feels that there may be any problem in section.will that have any effects on the IF stains.. Aazathraj. Technical Officer. Apollo Hospitals India. _________________________________________________________________ The latest songs, trailers and more http://video.in.msn.com/ From sbreeden <@t> nmda.nmsu.edu Wed Mar 17 07:04:10 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Mar 17 07:04:23 2010 Subject: [Histonet] Accessioning Similar Samples Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46F82@nmdamailsvr.nmda.ad.nmsu.edu> Regarding the need to avoid accessioning similar samples consecutively, I addressed this last week with my bosses. Specifically, I asked that spleen specimens not be accessioned consecutively (because of shattering and static and the possibility of fragments being carried over); my response was that I should just not CUT the blocks consecutively. Not the point I was trying to make but typical in some cases when addressing the issues histotechs face, true? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From DKBoyd <@t> chs.net Wed Mar 17 07:24:55 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Mar 17 07:25:02 2010 Subject: [Histonet] Accessioning Similar Samples In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F82@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Sara, Unfortunately the response you got is more common than I would like to see. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From JWeems <@t> sjha.org Wed Mar 17 09:21:34 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Mar 17 09:21:40 2010 Subject: [Histonet] Accessioning Similar Samples In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B02E46F82@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <27648A6C5BC9B145813DF5182F83AB4507590C@ITSSSXM01V1.one.ads.che.org> We have agar based markers - red, blue, green, yellow, orange, black, that we include in the block for similar tissues - as we have several that be separated. It is noted in the gross and confirmed at micro that the markers are present. After we've gone through the colors once, we put two, three, etc. It has worked well for us. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From cpyse <@t> x-celllab.com Wed Mar 17 10:00:35 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Mar 17 10:01:22 2010 Subject: [Histonet] Mc Coy fixative Message-ID: <000001cac5e2$99201da0$cb6058e0$@com> Hi Histonetters I am having a senior moment. Can anyone tell me what McCoy fixative is made of and what it is used for? Thanks in advance for the help. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com From pruegg <@t> ihctech.net Wed Mar 17 10:35:07 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Mar 17 10:36:17 2010 Subject: SPAM-LOW: [Histonet] Spencer AO 820 microtome questions In-Reply-To: References: Message-ID: Scott, You can look for the parts with some of the used equipment vendors, that "black beauty" was my favorite microtome of all times, it was heavy enough to cut plastic sections on when the new microtomes weren't, I am sure it would serve you well if you find a knife holder (you can purchase disposable blade holders that fit right in where the permanent blades went) and block holder. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott Parker Sent: Tuesday, March 16, 2010 12:20 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Spencer AO 820 microtome questions Dear all, I have access to a Spencer AO 820 microtome (a "black beauty" in Histo-parlance) that is does not have a disposable blade holder nor a chuck for holding paraffin cassettes. The microtome otherwise appears to be in good condition. I would like to get suggestions for where I might be able to purchase a chuck and disposable blade holder for this classic model. Alternatively, would it be a better idea for me to put this piece of equipment on display in a glass case and instead purchase a newer microtome. My work involves relatively low volume, basic sectioning of paraffin blocks for teaching and research. I don't need anything too fancy, just a microtome that is reliable and appropriate for student researchers to use. Thank you in advance for your responses. Scott Scott L. Parker, Ph.D. Assistant Professor Biology Coastal Carolina University P.O. Box 261954 Conway, SC 29528-6054 Office Phone: (843)-349-2491 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Mar 17 10:39:00 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Mar 17 10:40:01 2010 Subject: [Histonet] Re: proficiency testing for Muscle EHC- cont'd. In-Reply-To: <37E4BAC017F57141AF64FAA5AEB04CE8033A7AFC@wlmmsx01.nemours.org> References: <37E4BAC017F57141AF64FAA5AEB04CE8033A7AFC@wlmmsx01.nemours.org> Message-ID: Carol, I take these kinds of CAP issues to mean, develop your own program for proficiency testing and show how you have validated it, then show it to CAP, they usually want to know that you have a plan in place even if it is something you developed yourself. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Tuesday, March 16, 2010 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: proficiency testing for Muscle EHC- cont'd. Histonetter's I only received one reply on my question regarding "what everyone is doing for proficiency testing of enzyme HC staining for muscle"...espeically for CLIA.compliance, now that I will lose my indepent reviewer. My thanks to Jan Minchew for her reply. I am working from that for now, though my sitiation somewhat different. .....but, I am finding it particularly frustrating finding an answer to this question over the last three months or more, that I have searched everywhere from the histonet to the internet toevery friend I have doing EHC. Is it required under CLIA? My auditor says yes..so I am trying to comply, though I cannot fiind it specifically in any CLIA reg. Who is the CLIA expert out there...and why is this not included in HQIP? Can someone shed more light. Thx. CB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric.Hoy <@t> UTSouthwestern.edu Wed Mar 17 10:53:06 2010 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Wed Mar 17 10:53:11 2010 Subject: [Histonet] Re: Unsubscribing adventures In-Reply-To: <201003171501.o2HF1vft006445@nlpi121.prodigy.net> Message-ID: Several people have expressed frustration with the unsubscribe process. The process will not work unless you unsubscribe the same Email address that you used when you signed up for the list. If you have changed Email addresses, and the old address is forwarding to the new address, you will receive the mail, but when you unsubscribe under the new address, the listserv bot doesn't recognize it, and so nothing happens. Return with us now to those thrilling days of yesteryear, when you signed up for the list... What was your Email address back then? I hope this helps. Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== > Date: Tue, 16 Mar 2010 10:01:22 -0700 > From: "Vanessa Avalos" > Subject: [Histonet] UNSUBSCRIBE > To: "'HISTONET LISTS'" > Message-ID: <001001cac52a$4ef4ba00$ecde2e00$@com> > Content-Type: text/plain; charset="UTF-8" > > I can only speak for myself but yes, I am aware of all steps to be taken in > unsubscribing. Have followed them more than once but have been unsuccessful. > So yes, my frustration as lead me to write IN ALL CAPS!!!!! :) > From gagnone <@t> KGH.KARI.NET Wed Mar 17 11:13:55 2010 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Mar 17 11:14:05 2010 Subject: [Histonet] HPS Stain Message-ID: Hi Sheila, HPS (Hematoxylin-Phloxine-Saffron) is the routine stain we use. There are a few institutions in Canada that also still use this stain; around Ottawa and a few other places in Ontario and Quebec. Perhaps one of these institutions sent slides to your pathologist for referral/consult? It is quite a nice trichrome stain, but somewhat more complex and expensive, uses more staining dishes, and it's sometimes tricky to get a proper phloxine/saffron balance. Due to the use of H&E being more widespread, our pathology residents usually ask for H&E slides for their files when preparing for exams. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From dmccaig <@t> ckha.on.ca Wed Mar 17 11:18:03 2010 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed Mar 17 11:18:33 2010 Subject: [Histonet] HPS Stain In-Reply-To: Message-ID: I think there is confusion regarding the original question posed. They were not asking about the HPS stain. Some hospitals use an eosin/saffron stain in their routine H&E. I had another lab recently asking about the formula to prepare this solution instead of eosin. Any advise? Diana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Wednesday, March 17, 2010 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HPS Stain Hi Sheila, HPS (Hematoxylin-Phloxine-Saffron) is the routine stain we use. There are a few institutions in Canada that also still use this stain; around Ottawa and a few other places in Ontario and Quebec. Perhaps one of these institutions sent slides to your pathologist for referral/consult? It is quite a nice trichrome stain, but somewhat more complex and expensive, uses more staining dishes, and it's sometimes tricky to get a proper phloxine/saffron balance. Due to the use of H&E being more widespread, our pathology residents usually ask for H&E slides for their files when preparing for exams. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hymclab.hymclab <@t> ministryhealth.org Wed Mar 17 11:23:57 2010 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Wed Mar 17 11:24:04 2010 Subject: [Histonet] RE: CAP cancer protocols and checklists In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF0567DDC388@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF0567DDC388@EXCHANGESB> Message-ID: We have the Meditech Computer system and we have built a data section between Micro and Diagnosis namde Synoptic Tumor Protocol nd have canned texts available for all tumor types that pull in the required information needed. All the Pathologist's have to do is enter the information and hit enter and it goes to the next field needed to fill in. That way it is done consistently and within the CAP protocols. Dawn D. Schneider, HT(ASCP) Lead Histology Tech Howard Young Medical Center 240 Maple St. Woodruff, WI 54568 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Tuesday, March 16, 2010 4:20 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP cancer protocols and checklists I am interested in how you are implementing the CAP cancer protocols in your laboratory? Are you using synoptic reporting, dictating from the checklists, etc? Any information you can provide would be greatly appreciated. Thank you in advance for your comments and input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From clqsousa <@t> gmail.com Wed Mar 17 11:30:51 2010 From: clqsousa <@t> gmail.com (Carol Bain) Date: Wed Mar 17 11:30:57 2010 Subject: [Histonet] Technovit plastics and Exakt System Message-ID: <84bfdcb01003170930o3d3bbb7ax685183797a16b249@mail.gmail.com> Hi all, I just joined this mailing list, and I wonder if there is a subgroup or another list for people who work often with the Exakt cutting and grinding system. I use mainly Technovit 7200 and work with a lot of bone samples with some kind of biomaterial. Recently I started working with stented arteries, and had GREAT help with some issues from Gayle Callis and Lori Garcia. Thank you again girls! So, if there is a list or a group for "those people", where is it, or what is the URL? If not, I propose that we form one so we can help each other with these interesting techniques. I am sure I am not the only one who has had more than one "scratching the head" moments with those! What do you think? I am not sure if you can reply to this directly to my e-mail or if it has to go to the histonet group. My gmail is clqsousa. Thanks! Carol Bain Technical Research Assistant Histopathology Services Laboratory Comparative Pathology Deartment - Purdue University. (765) 494 0581 From JWeems <@t> sjha.org Wed Mar 17 11:52:46 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Mar 17 11:52:52 2010 Subject: [Histonet] RE: CAP cancer protocols and checklists In-Reply-To: References: <50DA0C6B72976B4AB3A0FCA04CC73DBF0567DDC388@EXCHANGESB> Message-ID: <27648A6C5BC9B145813DF5182F83AB45075945@ITSSSXM01V1.one.ads.che.org> Here, the pathologist or the transcriptionist inserts the table, which has been typed into Word and saved as an autocorrect or a Dragon template, into the report. We used them for years and have updated according to the new standards this year. Is anyone using the tables from the CAP website? I have asked how to get them into our reports but seems we need to use the autocorrect approach with our system. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, March 17, 2010 12:24 To: 'Carol Bryant'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP cancer protocols and checklists We have the Meditech Computer system and we have built a data section between Micro and Diagnosis namde Synoptic Tumor Protocol nd have canned texts available for all tumor types that pull in the required information needed. All the Pathologist's have to do is enter the information and hit enter and it goes to the next field needed to fill in. That way it is done consistently and within the CAP protocols. Dawn D. Schneider, HT(ASCP) Lead Histology Tech Howard Young Medical Center 240 Maple St. Woodruff, WI 54568 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Tuesday, March 16, 2010 4:20 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP cancer protocols and checklists I am interested in how you are implementing the CAP cancer protocols in your laboratory? Are you using synoptic reporting, dictating from the checklists, etc? Any information you can provide would be greatly appreciated. Thank you in advance for your comments and input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From talulahgosh <@t> gmail.com Wed Mar 17 13:23:39 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Mar 17 13:23:43 2010 Subject: [Histonet] ammonium chloride in ihc Message-ID: Hello all, We are trying a new antibody and the protocol from the company suggests a 50 mM ammonium chloride wash before blocking and adding the primary antibody. What is the purpose of this--antigen retrieval, autofluorescent quenching, something else? We were thinking about trying this step as our protocol does not yield any staining. Emily A state-imposed metaphysic or religion should be opposed, if necessary at pistol-point. We must fight for variety if we fight at all. The uniform is as dull as a sculptured egg. --Lawrence Durrell, Consequential Data, "Balthazar" From NSEARCY <@t> swmail.sw.org Wed Mar 17 13:57:44 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Wed Mar 17 13:57:52 2010 Subject: [Histonet] Sakura MicroArray Apparatus Message-ID: <4BA0DFD8.5D38.00EF.0@swmail.sw.org> We are making our own research / immunohistochemistry controls and have found that the tips, as well as the molds are becoming quite expensive. The tips ( especially in the hands of a resident) have a tendency to be rather "soft" and loose their shape rather quickly. Any other users having this issue? Anyone know of someone that provides this service for a fee? We would provide tissues, pay for block making. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From dancingwing <@t> yahoo.com Wed Mar 17 13:58:00 2010 From: dancingwing <@t> yahoo.com (Li Zhang) Date: Wed Mar 17 13:58:04 2010 Subject: [Histonet] question about mouse perfusion Message-ID: <411123.94087.qm@web111514.mail.gq1.yahoo.com> Dear all, I have just started to learn how to do perfusion in mice and this site has helped me a lot. We don't have the pump or Perfusion one in the lab, and probably won't be able to get one in the near future, so I'm using the hand injection method. I'm using a 60 ml syringe with 21G needle. I am doing perfusion in order to do immunofluorescence on the brain. My question is: can anyone give me a rough idea of how fast I should inject ( like ml/min). I think I've tried like 30 ml in 3 min, and I suspect that it's too fast because I do observe tissue swelling sometimes. And another question, which of the following is better for perfsusion: 1) PBS and then 4% PFA 2) Saline and then 4% PFA 3) sucrose--> saline--> PFA? Thank you very much for your help! From MAUGER <@t> email.chop.edu Wed Mar 17 14:31:07 2010 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Wed Mar 17 14:33:34 2010 Subject: [Histonet] CD3 on mouse Message-ID: <443F5B475A9BF647AB962E834884EBAD2786DA4E62@EX7CCRPW03V1.chop.edu> Hi All, Does anyone know a CD3 antibody that works well on FFPE mouse tissue? Thanks in advance, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From liz <@t> premierlab.com Wed Mar 17 14:38:26 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Mar 17 14:38:31 2010 Subject: [Histonet] CD3 on mouse In-Reply-To: <443F5B475A9BF647AB962E834884EBAD2786DA4E62@EX7CCRPW03V1.chop.edu> Message-ID: Dako's rabbit anti-CD3 cross reacts with mouse Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mauger, Joanne Sent: Wednesday, March 17, 2010 1:31 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD3 on mouse Hi All, Does anyone know a CD3 antibody that works well on FFPE mouse tissue? Thanks in advance, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Wed Mar 17 15:05:29 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Mar 17 15:05:41 2010 Subject: [Histonet] question about mouse perfusion In-Reply-To: <411123.94087.qm@web111514.mail.gq1.yahoo.com> References: <411123.94087.qm@web111514.mail.gq1.yahoo.com> Message-ID: <169842FA69A3A706690C6995@CDYwxp1931.ad.med.buffalo.edu> Hi Li, Try 1-2 ml/min perfusion rate. Yes 10ml/min is way too fast. I know it's tricky; I've done it by hand and by pump. Perfuse with PBS or Saline (same thing) first, then 4% PFA til mouse is stiff, then after taking the brain out immerse it in increasing grades of sucrose (10%-->20%-->30%) at 4C til brain sinks to the bottom of the vessel each time. Takes longer to sink with increasing grades - I think if I remember correctly in 10% it's only half an hour, 20% is a few hours, and 30% is overnight. Something like that. Some people skip the 20% and just do 10% and 30%. Some people just do 30%. Sucrose helps cryopreserve the delicate brain tissue. Regards, Merced --On Wednesday, March 17, 2010 11:58 AM -0700 Li Zhang wrote: > Dear all, > > I have just started to learn how to do perfusion in mice and this site > has helped me a lot. > > We don't have the pump or Perfusion one in the lab, and probably won't be > able to get one in the near future, so I'm using the hand injection > method. I'm using a 60 ml syringe with 21G needle. I am doing perfusion > in order to do immunofluorescence on the brain. > > My question is: can anyone give me a rough idea of how fast I should > inject ( like ml/min). I think I've tried like 30 ml in 3 min, and I > suspect that it's too fast because I do observe tissue swelling > sometimes. > > And another question, which of the following is better for perfsusion: 1) > PBS and then 4% PFA 2) Saline and then 4% PFA 3) sucrose--> saline--> > PFA? > > Thank you very much for your help! > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Bill.Tench <@t> pph.org Wed Mar 17 15:47:25 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Mar 17 15:47:35 2010 Subject: [Histonet] technologist productivity Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02862E8A@MAIL1.pph.local> I apologize in advance if I offend anyone with this inevitably touchy question. In the last two years we have lost one "older" histotechnologist, and the routine reliable services of another of that group and are now facing the issue of what can be expected from their replacements. I have little feel for this other than my experience with these and other previous employees, so I am hoping that some of you would be willing to share your thoughts/data with me either in response on this listserv, or alternatively "off line" directly to my email address. I am interested in some numbers on how many blocks one could expect to have embedded within an hour, with break down for simple larger or single pieces vs multiple small bx's like GI bx's or prostate needle bx's. I am looking for similar information in regard to the number of sections one could expect to be cut in an hour from routine blocks (and for this purpose, small and large bxs should be included in the same analysis-there is so much variation in regard to the number of sections that labs routinely cut from "smalls", so just a general mix of the number of slides would be helpful.) Thank you in advance for sharing-we are just trying to maintain reasonable expectations from our new/replacement technologists. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From skelemen <@t> temple.edu Wed Mar 17 17:05:05 2010 From: skelemen <@t> temple.edu (Sheri Kelemen) Date: Wed Mar 17 17:05:11 2010 Subject: [Histonet] tissue processing Message-ID: <4BA15211.3050904@temple.edu> Hi everyone, I need some advice. I have been doing tissue processing (on an old Shandon Hypercenter), embedding and sectioning for 15 yrs. and never really had any problems. I recently processed mouse tissues on a brand new tissue processor (Leica ASP300S). When I went to cut 5 micron sections the tissues (spleen, heart, aorta and lung) were very dry and brittle. They made what looked like sawdust after each cut. Soaking them in cold ammonia water helped some; but they still did not cut nicely. Here is my protocol: I perfusion fixed the mouse with fresh 10% Neutral Buffered Formalin (NBF). Then I removed the tissues and cut them in smaller pieces (no more that 5mm thick) and put them in 10% NBF for 24 hrs., then washed with PBS and stored in 70% ethanol (made with PBS) for 2 days before processing (only because I didn't have time to process till then). My processing protocol is as follows: 70% ETOH x1 1hr. 95% ETOH x2 1hr each 100% ETOH x3 1hr each Xylene x3 1hr each wax x3 45 min each 60?C Can any one tell me why they think my tissues are so so dry. What could I change for the next time I do this? Thank you. Sheri Kelemen Research Associate Cardiovascular Research Center, Temple University 3500 N. Broad St. Philadelphia, PA 19140 215-707-3170 work skelemen@temple.edu From liz <@t> premierlab.com Wed Mar 17 17:23:35 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Mar 17 17:26:16 2010 Subject: [Histonet] tissue processing References: <4BA15211.3050904@temple.edu> Message-ID: Sheri Your processing cycle is too long. We process 20 minutes per station for mouse tissue only 2 absolutes and 2 xylenes and 3 paraffins. Even 3 absolutes and 3 xylenes at 20 minutes a station we find over processes the tissue. Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sheri Kelemen Sent: Wed 3/17/2010 4:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue processing Hi everyone, I need some advice. I have been doing tissue processing (on an old Shandon Hypercenter), embedding and sectioning for 15 yrs. and never really had any problems. I recently processed mouse tissues on a brand new tissue processor (Leica ASP300S). When I went to cut 5 micron sections the tissues (spleen, heart, aorta and lung) were very dry and brittle. They made what looked like sawdust after each cut. Soaking them in cold ammonia water helped some; but they still did not cut nicely. Here is my protocol: I perfusion fixed the mouse with fresh 10% Neutral Buffered Formalin (NBF). Then I removed the tissues and cut them in smaller pieces (no more that 5mm thick) and put them in 10% NBF for 24 hrs., then washed with PBS and stored in 70% ethanol (made with PBS) for 2 days before processing (only because I didn't have time to process till then). My processing protocol is as follows: 70% ETOH x1 1hr. 95% ETOH x2 1hr each 100% ETOH x3 1hr each Xylene x3 1hr each wax x3 45 min each 60?C Can any one tell me why they think my tissues are so so dry. What could I change for the next time I do this? Thank you. Sheri Kelemen Research Associate Cardiovascular Research Center, Temple University 3500 N. Broad St. Philadelphia, PA 19140 215-707-3170 work skelemen@temple.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jag93 <@t> cam.ac.uk Wed Mar 17 18:05:54 2010 From: jag93 <@t> cam.ac.uk (Andrew Gillis) Date: Wed Mar 17 18:06:01 2010 Subject: [Histonet] Vibratome skipping sections Message-ID: <4BA16052.9040009@cam.ac.uk> Hello, I have just started using a vibratome (Leica VT1000S) to cut sections of fish embryos after whole mount in situ hybridization, but I've encountered a problem with this. I have been setting the vibratome to cut sections of 50um to 70um in thickness (on the recommendation of a colleague who routinely uses this instrument), and I've noticed that after a few nice sections, the machine starts skipping a section (i.e. the blade will just pass over the block). When this happens, the next section to be cut is approximately twice as thick (i.e. if my section thickness is set to 70um, the blade will miss a section, and will then cut a section that is approxaimtely 140um thick). I'm not sure if this is a problem with the settings I'm using, or with the way I prepare the blocks.... I tend to cut with a speed of 5-7, and a frequency of ~8 (again, based on settings recommended by a colleague). To prepare the block, I embed the embryos in 30% gelatin in PBS, and once set, I trim it and fix the block in 4% PFA for 48hrs. I try not to make my blocks too tall, and they seem nice and firm when I mount them on the machine.... There are certainly a lot of variables that I could play with here - and I could probably spend weeks fiddling with it! I would really appreciate hearing from anyone else has experienced this, and who has ideas of how to fix it. Thank you very much, Andrew From cmmathis1 <@t> bellsouth.net Wed Mar 17 20:15:11 2010 From: cmmathis1 <@t> bellsouth.net (RICKY MATHIS) Date: Wed Mar 17 20:15:18 2010 Subject: [Histonet] Anti-human abs work on porcine tissue Message-ID: <28389.22216.qm@web180613.mail.sp1.yahoo.com> I work with some porcine tissues and it is sometimes difficult to find antibodies specific to pig.??A doctor?I work with asked some one he knew in Europe about using antibodies that are listed as working in human being used on porcine tissue.? This person?stated that there would be about a 10% chance that the anti-human antibodies would work on porcine tissues.?? The antibody companies mostly give the same "we did not try it on pig tissue" response.? I understand that it is likely expensive to continue to test antibodies on a wide variety of animal tissues, so that is fine.? But what do you guys think about the 10% chance?? I would have said more than that. Thank you in advance for your time, Cathy From anonwums1 <@t> gmail.com Wed Mar 17 20:38:01 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Wed Mar 17 20:38:06 2010 Subject: [Histonet] Anti-human abs work on porcine tissue In-Reply-To: <28389.22216.qm@web180613.mail.sp1.yahoo.com> References: <28389.22216.qm@web180613.mail.sp1.yahoo.com> Message-ID: <858249121003171838i658b1buc739e75ac4887abc@mail.gmail.com> I routine use anti-human antibodies to stain mouse tissue, so it can be done. It completely depends on the antigen they used to immunize. If the human and pig proteins are highly homologous or identical, then it has a good chance of working. If they're not, you're in uncharted territories. If you ask nicely, most companies will tell you what antigen or antigen fragment they used, but most don't actively advertise this. Adam On Wed, Mar 17, 2010 at 8:15 PM, RICKY MATHIS wrote: > I work with some porcine tissues and it is sometimes difficult to find > antibodies specific to pig. A doctor I work with asked some one he knew in > Europe about using antibodies that are listed as working in human being used > on porcine tissue. This person stated that there would be about a 10% > chance that the anti-human antibodies would work on porcine tissues. The > antibody companies mostly give the same "we did not try it on pig tissue" > response. I understand that it is likely expensive to continue to test > antibodies on a wide variety of animal tissues, so that is fine. But what > do you guys think about the 10% chance? I would have said more than that. > Thank you in advance for your time, > Cathy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From alexandra.meinl <@t> gmail.com Thu Mar 18 04:02:16 2010 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Thu Mar 18 04:08:32 2010 Subject: [Histonet] Anti-human abs work on porcine tissue In-Reply-To: <858249121003171838i658b1buc739e75ac4887abc@mail.gmail.com> References: <28389.22216.qm@web180613.mail.sp1.yahoo.com> <858249121003171838i658b1buc739e75ac4887abc@mail.gmail.com> Message-ID: I work on porcine tissue regularly and I can't confirm the 10%. Maybe I accidentially used only the highly conserved proteins, I didn't check on that. But I usually try anti-human antibodies and most of the time they work really well! Alexandra ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.meinl@trauma.lbg.ac.at From mdeguzman <@t> lifecell.com Thu Mar 18 05:27:46 2010 From: mdeguzman <@t> lifecell.com (DeGuzman, Maria) Date: Thu Mar 18 05:29:22 2010 Subject: [Histonet] how to process calcified bone for cryomicrotomy In-Reply-To: <18b270f0-1d34-4035-9610-d6455c4b83ec@amwpht01.kci.com> References: <18b270f0-1d34-4035-9610-d6455c4b83ec@amwpht01.kci.com> Message-ID: <5476245379016B4D8212E8BCD11ECFFBB5F0E9E4@AMWPVEX01.kci.com> Hello Fellow Histologists, I am writing because I need information on how to process calcified bone for cryomicrotomy. I am working on an animal bone study that will require cryosectioning of calcified bone to stain for fat (Oil Red O). Does anyone have any information on this topic that would like to share? A procedure, cryostat used, and mounting medium. Thanks in advance, Fanny Maria V. De Guzman| Histology Technician I Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1482 Email mdeguzman@lifecell.com Mobile 732.688.1386 www.Lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 17, 2010 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. UNSUBSCRIBE (Vanessa Avalos) 2. Exciting Histotech Job in New England!! (Alisha Dynan) 3. Re: UNSUBSCRIBE (Kim.Donadio@bhcpns.org) 4. HT/HTL position Gainesville. FL (Konni Black) 5. Re: proficiency testing for Muscle EHC- cont'd. (Barone, Carol ) 6. Spencer AO 820 microtome questions (Scott Parker) 7. Job Openings in Phoenix, Arizona (Turner, Mark) 8. Lab Assistant Position in Phoenix, AZ (Turner, Mark) 9. Saffron/Eosin as a counterstain for H&E??? (sheila adey) 10. RE: Saffron/Eosin as a counterstain for H&E??? (Liz Chlipala) 11. NY License (Jeffrey Silverman) 12. (no subject) (Jeffrey Silverman) 13. CAP cancer protocols and checklists (Carol Bryant) 14. (no subject) (Jeffrey Silverman) 15. Re: Removing Yellow color from slides refixed in Bouin's solution (Joseph Saby) 16. RE: (no subject) (Sherwood, Margaret ) 17. RE: (no subject) (WILLIAM DESALVO) 18. Re: Spencer AO 820 microtome questions (Michael Folsom) 19. Re: Saffron/Eosin as a counterstain for H&E??? (John Kiernan) 20. RE: Superfrost Plus Slides (Cathy) 21. problem in IF stains (Aazath Raj) 22. Accessioning Similar Samples (Breeden, Sara) 23. Re: Accessioning Similar Samples (DKBoyd@chs.net) 24. RE: Accessioning Similar Samples (Weems, Joyce) 25. Mc Coy fixative (Cynthia Pyse) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Mar 2010 10:01:22 -0700 From: "Vanessa Avalos" Subject: [Histonet] UNSUBSCRIBE To: "'HISTONET LISTS'" Message-ID: <001001cac52a$4ef4ba00$ecde2e00$@com> Content-Type: text/plain; charset="UTF-8" I can only speak for myself but yes, I am aware of all steps to be taken in unsubscribing. Have followed them more than once but have been unsuccessful. So yes, my frustration as lead me to write IN ALL CAPS!!!!! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, March 16, 2010 7:53 AM To: Dimitri Scholz Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) both of you are not allowed to use the internet anymore. because you can't follow instructions. WE CAN'T UNSUBSCRIBE YOU. ONLY YOU CAN. A state-imposed metaphysic or religion should be opposed, if necessary at pistol-point. We must fight for variety if we fight at all. The uniform is as dull as a sculptured egg. --Lawrence Durrell, Consequential Data, "Balthazar" On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz wrote: > Me too! > > Dimitri Scholz, PhD, Dr. Sci. > Director of Biological Imaging > Conway Institute > University College Dublin (UCD) > Belfield, Dublin 4 > Ireland > Tel: +353-1 716 6736 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa > Gutierrez > Sent: 16 March 2010 14:21 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM > ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE > RULES GETS ME OFF! > > THANK YOU > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: 16 Mar 2010 13:40:23 -0400 From: Alisha Dynan Subject: [Histonet] Exciting Histotech Job in New England!! To: histonet@lists.utsouthwestern.edu Message-ID: <1516952045.1268761223703.JavaMail.cfservice@webserver54> Content-Type: text/plain; charset="utf-8" Hi Histotechs, I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great job opportunities in various areas across the country. One particular client that I am working with is a financially sound, medically advanced, full-service hospital in New England. My client is looking for both a HTL(ASCP) certified histotechnologist and a HT(ASCP) certified histotech for their hospital. Both positions would work a day shift. My client is offering a very competitive compensation package, full benefits, and relocation assistance. If interested, please email me a copy of your resume and follow-up with a call to learn more! Below is a list of some of the great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Opportunities: CT - Histology Operations Manager NY - New York City - Histotech 3rd shift NV -Las Vegas - Histotech 3rd shift NV Las Vegas - Histology Supervisor - 3rd shift PA - Cytology Supervisor CA - Palm Springs - Histotech - 1st shift CA - Southern - Histotech VA - Histotech 2nd shift NH - HTL and HT GA - Atlanta - Histotech GA - Atlanta - Pathology Coordinator (HT or CT) NV - Pathologist Assistant TN - Pathologist Assistant ND - Pathologist Assistant GA - Pathologist Assistant If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 3 Date: Tue, 16 Mar 2010 12:47:31 -0500 From: Kim.Donadio@bhcpns.org Subject: Re: [Histonet] UNSUBSCRIBE To: "Vanessa Avalos" Cc: 'HISTONET LISTS' , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I have a suggestion. If you are extremely frustrated with getting all the emails that come from here and you are having a hard time unsubscribing, perhaps you could put a block on histonet so you cant receive emails from them until you get unsubscribed? Just a thought. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Vanessa Avalos" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/16/2010 12:01 PM To "'HISTONET LISTS'" cc Subject [Histonet] UNSUBSCRIBE I can only speak for myself but yes, I am aware of all steps to be taken in unsubscribing. Have followed them more than once but have been unsuccessful. So yes, my frustration as lead me to write IN ALL CAPS!!!!! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, March 16, 2010 7:53 AM To: Dimitri Scholz Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) both of you are not allowed to use the internet anymore. because you can't follow instructions. WE CAN'T UNSUBSCRIBE YOU. ONLY YOU CAN. A state-imposed metaphysic or religion should be opposed, if necessary at pistol-point. We must fight for variety if we fight at all. The uniform is as dull as a sculptured egg. --Lawrence Durrell, Consequential Data, "Balthazar" On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz wrote: > Me too! > > Dimitri Scholz, PhD, Dr. Sci. > Director of Biological Imaging > Conway Institute > University College Dublin (UCD) > Belfield, Dublin 4 > Ireland > Tel: +353-1 716 6736 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa > Gutierrez > Sent: 16 March 2010 14:21 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM > ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE > RULES GETS ME OFF! > > THANK YOU > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 4 Date: Tue, 16 Mar 2010 10:59:16 -0700 From: "Konni Black" Subject: [Histonet] HT/HTL position Gainesville. FL To: "histonet" Message-ID: Content-Type: text/plain; charset="Windows-1252" Fulltime HT/HTL opening in GI lab in Gainesville, Florida. Must have 3-5 years experience. Great lab. Flexible work schedule. Contact Konni Black at kblack@digestivehlth.com or 253-503-2560. ------------------------------ Message: 5 Date: Tue, 16 Mar 2010 14:10:50 -0400 From: "Barone, Carol " Subject: [Histonet] Re: proficiency testing for Muscle EHC- cont'd. To: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7AFC@wlmmsx01.nemours.org> Content-Type: text/plain; charset="us-ascii" Histonetter's I only received one reply on my question regarding "what everyone is doing for proficiency testing of enzyme HC staining for muscle"...espeically for CLIA.compliance, now that I will lose my indepent reviewer. My thanks to Jan Minchew for her reply. I am working from that for now, though my sitiation somewhat different. .....but, I am finding it particularly frustrating finding an answer to this question over the last three months or more, that I have searched everywhere from the histonet to the internet toevery friend I have doing EHC. Is it required under CLIA? My auditor says yes..so I am trying to comply, though I cannot fiind it specifically in any CLIA reg. Who is the CLIA expert out there...and why is this not included in HQIP? Can someone shed more light. Thx. CB ------------------------------ Message: 6 Date: Tue, 16 Mar 2010 14:20:02 -0400 From: Scott Parker Subject: [Histonet] Spencer AO 820 microtome questions To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dear all, I have access to a Spencer AO 820 microtome (a "black beauty" in Histo-parlance) that is does not have a disposable blade holder nor a chuck for holding paraffin cassettes. The microtome otherwise appears to be in good condition. I would like to get suggestions for where I might be able to purchase a chuck and disposable blade holder for this classic model. Alternatively, would it be a better idea for me to put this piece of equipment on display in a glass case and instead purchase a newer microtome. My work involves relatively low volume, basic sectioning of paraffin blocks for teaching and research. I don't need anything too fancy, just a microtome that is reliable and appropriate for student researchers to use. Thank you in advance for your responses. Scott Scott L. Parker, Ph.D. Assistant Professor Biology Coastal Carolina University P.O. Box 261954 Conway, SC 29528-6054 Office Phone: (843)-349-2491 ------------------------------ Message: 7 Date: Tue, 16 Mar 2010 11:52:43 -0700 From: "Turner, Mark" Subject: [Histonet] Job Openings in Phoenix, Arizona To: "histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histonetters! Caris MPI, a division of Caris Life Sciences, is looking for 2 histotechs to assist with a dramatically increasing workflow. We are a very dynamic IHC lab which is growing exponentially. The positions are day shift, Monday through Friday, with occasional weekends. The successful candidates will have HT (ASCP) certification, 1-2 years IHC experience, preferably with the Ventana and Dako platforms, and will enjoy being part of a fast paced, quality oriented team. Qualification in Immunohistochemistry (QIHC) is a plus. Caris MPI is located in sunny Phoenix, AZ and will be moving to a brand new lab in May 2010. Equipment is state of the art, and there is a corporate commitment toward quality. Caris has had a presence in the Phoenix area for 3 years and is continuing to grow. The area is the 5th largest metropolitan in the country and has available anything you need to make life more interesting. If you would like to grow with us, please submit your resume in confidence to mturner@carisdx.com. Mark Turner, Ph. D., HT(ASCP) QIHC Supervisor IHC Target Now MPI Caris Life Sciences 445 N. 5th Street Phoenix, AZ 85004 Cell: 602-309-5084 Direct 602-358-8913 Fax: 602-358-8919 mturner@carisdx.com ------------------------------ Message: 8 Date: Tue, 16 Mar 2010 11:57:47 -0700 From: "Turner, Mark" Subject: [Histonet] Lab Assistant Position in Phoenix, AZ To: "histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Caris MPI, a division of Caris Life Sciences, is looking to hire a Lab Assistant for 2nd shift. This will be a support position for our team of histology professionals, and is a great entry level position into the laboratory setting. Some college is preferred, with a focus on the sciences. Opportunities for advancement abound, as this position is open due to our previous lab assistant moving into a histotech role. Caris MPI is located in sunny Phoenix, AZ and will be moving to a brand new lab in May 2010. Equipment is state of the art, and there is a corporate commitment toward quality. Caris has had a presence in the Phoenix area for 3 years and is continuing to grow. The area is the 5th largest metropolitan in the country and has available anything you need to make life more interesting. If you would like to grow with us, please submit your resume in confidence to mturner@carisdx.com. Mark Turner, Ph. D., HT(ASCP) QIHC Supervisor IHC Target Now MPI Caris Life Sciences 445 N. 5th Street Phoenix, AZ 85004 Cell: 602-309-5084 Direct 602-358-8913 Fax: 602-358-8919 mturner@carisdx.com ------------------------------ Message: 9 Date: Tue, 16 Mar 2010 15:04:23 -0400 From: sheila adey Subject: [Histonet] Saffron/Eosin as a counterstain for H&E??? To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, Our pathologist said that he say H&E slides with a counterstain that had some amount of saffron in it? Is anyone familiar with this? Sheila Adey HT MLT _________________________________________________________________ Stay in touch. http://go.microsoft.com/?linkid=9712959 ------------------------------ Message: 10 Date: Tue, 16 Mar 2010 13:30:38 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] Saffron/Eosin as a counterstain for H&E??? To: "sheila adey" , Message-ID: Content-Type: text/plain; charset="us-ascii" Its hematoxylin, ploxine and saffron. Believe it or not it was the stain that we used at my first job back in 1973. We also fixed in Zenkers too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Tuesday, March 16, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Saffron/Eosin as a counterstain for H&E??? Hello, Our pathologist said that he say H&E slides with a counterstain that had some amount of saffron in it? Is anyone familiar with this? Sheila Adey HT MLT _________________________________________________________________ Stay in touch. http://go.microsoft.com/?linkid=9712959_________________________________ ______________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 16 Mar 2010 14:07:54 -0700 (PDT) From: Jeffrey Silverman Subject: [Histonet] NY License To: rmoody@ameripath.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <12350.35531.qm@web111103.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii New York State Education Department- google it and you'll see a link for licensed professionals. Jeff Silverman ------------------------------ Message: 12 Date: Tue, 16 Mar 2010 14:14:57 -0700 (PDT) From: Jeffrey Silverman Subject: [Histonet] (no subject) To: cscampbe@uci.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <161299.62124.qm@web111104.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii Don't know what you mean by rinses, but 5 to at most 10 minutes in gently running tap water should do the trick. If not, the lithium carbonate is what's prescribed. Jeff Silverman ------------------------------ Message: 13 Date: Tue, 16 Mar 2010 17:19:33 -0400 From: Carol Bryant Subject: [Histonet] CAP cancer protocols and checklists To: "Histonet@lists.utsouthwestern.edu" Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF0567DDC388@EXCHANGESB> Content-Type: text/plain; charset="us-ascii" I am interested in how you are implementing the CAP cancer protocols in your laboratory? Are you using synoptic reporting, dictating from the checklists, etc? Any information you can provide would be greatly appreciated. Thank you in advance for your comments and input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ------------------------------ Message: 14 Date: Tue, 16 Mar 2010 14:23:45 -0700 (PDT) From: Jeffrey Silverman Subject: [Histonet] (no subject) To: Allison_Scott@hchd.tmc.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <190750.38557.qm@web111106.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Allison- That's our policy- don't accession like biopsies back to back. But if it is unavoidable, you can mark the second like biopsy case with a dab of yellow margin ink on at least one piece in each block and dictate that into the gross.? ie One fragment is marked in yellow for positive patient identification. Jeff Silverman- busy PA ------------------------------ Message: 15 Date: Tue, 16 Mar 2010 14:25:01 -0700 (PDT) From: Joseph Saby Subject: Re: [Histonet] Removing Yellow color from slides refixed in Bouin's solution To: Cynthia Pyse , cscampbe@uci.edu, HistoNet Message-ID: <619715.30232.qm@web113816.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii Running tap water for 10 minutes should do the trick. Joe Saby ________________________________ From: Cynthia Pyse To: cscampbe@uci.edu; HistoNet Sent: Tue, March 16, 2010 10:52:20 AM Subject: RE: [Histonet] Removing Yellow color from slides refixed in Bouin's solution I just place the slides into 80% ETOH for 2 minutes. Cindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cscampbe@uci.edu Sent: Monday, March 15, 2010 7:42 PM To: HistoNet Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's solution Hi Histonet, After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it very difficult to remove the yellow coloring. Rinses with water are not doing the trick. Does anyone have some advice on how to bring the slides back to a clear color so that I may proceed with the Masson Trichrome procedure? Thanks! -Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 16 Mar 2010 17:34:15 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] (no subject) To: "Jeffrey Silverman" , Cc: histonet@lists.utsouthwestern.edu Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E240DD@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" We also just wash in running tap water and the yellow stain disappears. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Tuesday, March 16, 2010 5:15 PM To: cscampbe@uci.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Don't know what you mean by rinses, but 5 to at most 10 minutes in gently running tap water should do the trick. If not, the lithium carbonate is what's prescribed. Jeff Silverman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 17 Date: Tue, 16 Mar 2010 18:33:50 -0600 From: WILLIAM DESALVO Subject: RE: [Histonet] (no subject) To: , Cc: histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" An additional method to use to create positive identification when accessioning and grossing like specimens together when you must or just to increase the indetification factor for all specimens: Use the Davidson marking system and have the grossing person rotate the colors used to mark margins or small tissue by case and/or specimen and of course dictate to specific color used. Once they are in the habit, no two cases or specimens will have the same ink color and you increase quality assurance. William DeSalvo, B.S., HTL(ASCP) > Date: Tue, 16 Mar 2010 14:23:45 -0700 > From: pathmaster@yahoo.com > To: Allison_Scott@hchd.tmc.edu > CC: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Hi Allison- > That's our policy- don't accession like biopsies back to back. > But if it is unavoidable, you can mark the second like biopsy case with a dab of yellow margin ink on at least one piece in each block and dictate that into the gross. ie One fragment is marked in yellow for positive patient identification. > > Jeff Silverman- busy PA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850553/direct/01/ ------------------------------ Message: 18 Date: Tue, 16 Mar 2010 18:53:05 -0600 From: Michael Folsom Subject: Re: [Histonet] Spencer AO 820 microtome questions To: Scott Parker Cc: histonet@lists.utsouthwestern.edu Message-ID: <1268787185.6216.14.camel@chico.322tulane.org> Content-Type: text/plain; charset="UTF-8" Scott: Does the chuck have a clamp in it? If so soak a piece of soft wood that fits in the chuck in 100% ETOH (pulling a vacuum on it would help) and transition into paraffin. Once its soaked in paraffin pull it out and allow to solidify. Your tissue will need to be flat embedded in a simple boat. Use a razor blade to cut it out but leave a good bit of paraffin behind and around it. Once its cool use an artists pallet knife (small) to melt the surface of the paraffin on the wood and the back of the block and weld in place with the melted paraffin. Re: the knife - they make a knife holder that holds either hi or low profile disposable blades which fits in the microtome's knife holder. One thing about the old AO - they are almost indestructible but never back up the crank because it can strip the gears. Good luck - Mike mwfolsom@rgbio.cm On Tue, 2010-03-16 at 14:20 -0400, Scott Parker wrote: > Dear all, > > I have access to a Spencer AO 820 microtome (a "black beauty" in > Histo-parlance) that is does not have a disposable blade holder nor a chuck > for holding paraffin cassettes. The microtome otherwise appears to be in > good condition. I would like to get suggestions for where I might be able to > purchase a chuck and disposable blade holder for this classic model. > Alternatively, would it be a better idea for me to put this piece of > equipment on display in a glass case and instead purchase a newer microtome. > My work involves relatively low volume, basic sectioning of paraffin blocks > for teaching and research. I don't need anything too fancy, just a microtome > that is reliable and appropriate for student researchers to use. > > Thank you in advance for your responses. > > Scott > > > Scott L. Parker, Ph.D. > > Assistant Professor Biology > > Coastal Carolina University > > P.O. Box 261954 > > Conway, SC 29528-6054 > > > > Office Phone: (843)-349-2491 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Tue, 16 Mar 2010 23:05:55 -0400 From: John Kiernan Subject: Re: [Histonet] Saffron/Eosin as a counterstain for H&E??? To: sheila adey Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; CHARSET=US-ASCII There is Garvey,W (1991): Modification of the Mayer hematoxylin stain. J. Histotechnol. 14, 163-165. The title is rather confusing. Her method included a modified composition of Mayer's haemalum (ethanol instead of chloral hydrate, and less alum), and instead of eosin Y the counterstain was a solution containing phloxin B (CI45410, Acid red 92) and saffron (which is CI 75100, Natural yellow 6). The author stated that saffron bought from a health food store was OK and much less expensive than that bought from a lab vendor. This counterstain was used by Rostoker et al (2001) Nephrology Dialysis Transplantation 16:513-517 in a study of intestinal pathology in glomerulonephritis. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: sheila adey Date: Tuesday, March 16, 2010 15:05 Subject: [Histonet] Saffron/Eosin as a counterstain for H&E??? To: histonet@lists.utsouthwestern.edu > > Hello, > > Our pathologist said that he say H&E slides with a counterstain > that had some amount of saffron in it? > > Is anyone familiar with this? > > Sheila Adey HT MLT > > > > _________________________________________________________________ > Stay in touch. > http://go.microsoft.com/?linkid=9712959_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 16 Mar 2010 20:34:43 -0700 From: "Cathy" Subject: RE: [Histonet] Superfrost Plus Slides To: "'Fahy, Claire'" , "'Lynn Lee'" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Claire, We experienced problems with our IHC staining the fall of 2008 and it was traced back to a problem with particular lot numbers of the Superfrost slides. It was difficult to track down due to the inconsistency of the problem and the variation in the problem. We finally spoke with our Ventana technical specialist and she was able to tie our problem to problems that other facilities were experiencing. You could try contacting your Ventana technical resource person; they may be able to help. One thing we found out was that the Superfrost slides are manufactured at one site and distributed to vendors under their own name. Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fahy, Claire Sent: Tuesday, March 16, 2010 4:05 AM To: Lynn Lee Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Superfrost Plus Slides Hi Lynn Yes, our instrument is decontaminated and cleaned as per manual. There was some old threads on Histonet where some people had got varying staining between runs so I was wondering if anybody had found a solution to this problem.Maybe it is simply the slides? -----Original Message----- From: Lynn Lee [mailto:lynnlee2010@live.com] Sent: 16 March 2010 10:43 To: Fahy, Claire Subject: RE: [Histonet] Superfrost Plus Slides You could have contamination in the instrument. Has it been decontaminated quarterly per the instruction manual? I have never heard of uneven staining from plus slides. > Date: Tue, 16 Mar 2010 10:16:38 +0000 > From: Claire.Fahy@agriculture.gov.ie > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Superfrost Plus Slides > > Hi > > I know this is in reply to an old threat but we are experiencing varying and uneven staining of our sections on the Ventana Benchmark.It is not confined to a perticular anitbody and doesn't happen all the time either. > > We have been told that it may be the slides.We currently use Thermo Scientific (Menzel-Glaser) superfrost plus slides.Does anyone use the Ventana IHC slides? > > Has anyone has experienced similar problems?If so,what have you done to resolve them. > > Thanks > > Claire fahy > Lab analyst, > Histopatholgy Lab > > Department of Agriculture, Fisheries and Food > > The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). > > An Roinn Talmha?ochta, Iascaigh agus Bia > > T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh ?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air, n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar ? do r?omhaire le do thoil. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. Learn More. ________________________________ Department of Agriculture, Fisheries and Food The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). An Roinn Talmha?ochta, Iascaigh agus Bia T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh ?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air, n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar ? do r?omhaire le do thoil. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 17 Mar 2010 10:20:39 +0530 From: Aazath Raj Subject: [Histonet] problem in IF stains To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear Friends, I am having a problem in IF stains on Renal Bx.In direct immunofloresence on the frozen sections does not have disered contrast and many of the cases its falsely negative. Can you people tell me the possible mistake which may cause these kind of effects on the IF stain. I dont see any gross mistakes on the Frozen sections after sectioning.All the temperature and other things are fine on the cryostate, but my pathologist feels that there may be any problem in section.will that have any effects on the IF stains.. Aazathraj. Technical Officer. Apollo Hospitals India. _________________________________________________________________ The latest songs, trailers and more http://video.in.msn.com/ ------------------------------ Message: 22 Date: Wed, 17 Mar 2010 06:04:10 -0600 From: "Breeden, Sara" Subject: [Histonet] Accessioning Similar Samples To: "histonet" Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46F82@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Regarding the need to avoid accessioning similar samples consecutively, I addressed this last week with my bosses. Specifically, I asked that spleen specimens not be accessioned consecutively (because of shattering and static and the possibility of fragments being carried over); my response was that I should just not CUT the blocks consecutively. Not the point I was trying to make but typical in some cases when addressing the issues histotechs face, true? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 23 Date: Wed, 17 Mar 2010 08:24:55 -0400 From: DKBoyd@chs.net Subject: Re: [Histonet] Accessioning Similar Samples To: "Breeden, Sara" Cc: histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Sara, Unfortunately the response you got is more common than I would like to see. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 24 Date: Wed, 17 Mar 2010 10:21:34 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Accessioning Similar Samples To: "histonet" Message-ID: <27648A6C5BC9B145813DF5182F83AB4507590C@ITSSSXM01V1.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We have agar based markers - red, blue, green, yellow, orange, black, that we include in the block for similar tissues - as we have several that be separated. It is noted in the gross and confirmed at micro that the markers are present. After we've gone through the colors once, we put two, three, etc. It has worked well for us. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 25 Date: Wed, 17 Mar 2010 11:00:35 -0400 From: "Cynthia Pyse" Subject: [Histonet] Mc Coy fixative To: Message-ID: <000001cac5e2$99201da0$cb6058e0$@com> Content-Type: text/plain; charset="us-ascii" Hi Histonetters I am having a senior moment. Can anyone tell me what McCoy fixative is made of and what it is used for? Thanks in advance for the help. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 24 **************************************** From shive003 <@t> umn.edu Thu Mar 18 07:48:53 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Mar 18 07:49:01 2010 Subject: [Histonet] Anti-human abs work on porcine tissue References: <28389.22216.qm@web180613.mail.sp1.yahoo.com> Message-ID: <6CDABB2487DB4002B801FBE12BCFC4AF@auxs.umn.edu> I work on all species, porcine being one of them. I've never done a % survey of the number of Abs made against human epitopes that also work on porcine tissue, but I can give you generalizations. All of the rabbit polyclonal antibodies that I've tried have cross-reacted with porcine tissue. The polyclonals that I use the most are the Abs against hormones, neuroendocrine markers, vasculature, astrocytes, etc. Most of the mouse monoclonal antibodies that I've tried have cross-reacted with porcine tissue. These would be the intermediate filaments, melanoma antigens, cell proliferating antigens, neuroendocrine markers, etc. You may have more difficulty with leukocyte markers (CD markers). They seem to have less homology with human markers than the above items, and not all cross-react, in my experience. You only learn this by trial and error, unfortunately, since data about species cross-reactivity is rare. I have 2 polyclonal and 2 monoclonal CD markers that cross-react with porcine. That is not to say that there aren't more, but that's all that I currently have worked up on porcine tissue. I have not tried Abs like ER, PR, CEA, TTF, etc. I've done species cross-reactivity studies with every antibody I have in stock (>80), so if you send me a list of the antibodies you're interested in (directly to me), I can let you know which ones cross-react with porcine tissue, and which vendor works for me. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "RICKY MATHIS" To: "histonet" Sent: Wednesday, March 17, 2010 8:15 PM Subject: [Histonet] Anti-human abs work on porcine tissue I work with some porcine tissues and it is sometimes difficult to find antibodies specific to pig. A doctor I work with asked some one he knew in Europe about using antibodies that are listed as working in human being used on porcine tissue. This person stated that there would be about a 10% chance that the anti-human antibodies would work on porcine tissues. The antibody companies mostly give the same "we did not try it on pig tissue" response. I understand that it is likely expensive to continue to test antibodies on a wide variety of animal tissues, so that is fine. But what do you guys think about the 10% chance? I would have said more than that. Thank you in advance for your time, Cathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Thu Mar 18 08:20:29 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Mar 18 08:20:29 2010 Subject: [Histonet] Histotech Job in New England! Message-ID: <1774878309.1268918429236.JavaMail.cfservice@webserver60> Hi Histotechs, I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great job opportunities in various areas across the country. One particular client that I am working with is a financially sound, medically advanced, full-service hospital in New England. My client is looking for both a HTL(ASCP) certified histotechnologist and a HT(ASCP) certified histotech for their hospital. Both positions would work a day shift. My client is offering a very competitive compensation package, full benefits, and relocation assistance. If interested, please email me a copy of your resume and follow-up with a call to learn more! Below is a list of some of the great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Opportunities: CT - Histology Operations Manager NY - New York City - Histotech 3rd shift NV -Las Vegas - Histotech 3rd shift NV Las Vegas - Histology Supervisor - 3rd shift PA - Cytology Supervisor CA - Palm Springs - Histotech - 1st shift CA - Southern - Histotech VA - Histotech 2nd shift NH - HTL and HT GA - Atlanta - Histotech GA - Atlanta - Pathology Coordinator (HT or CT) NV - Pathologist Assistant TN - Pathologist Assistant ND - Pathologist Assistant GA - Pathologist Assistant If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From carl.hobbs <@t> kcl.ac.uk Thu Mar 18 08:34:30 2010 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Mar 18 08:35:37 2010 Subject: [Histonet] Re: ammonium chloride in ihc Message-ID: <11D9615B89C10747B1C985966A63D7CA2D7BE53C77@KCL-MAIL04.kclad.ds.kcl.ac.uk> Used for quenching Formalin - induced fluorescence. Carl Hobbs Histology Manager Wolfson Centre for Age-Related Diseases King's College London Tel.020 7848 6810 From alexandra.meinl <@t> gmail.com Thu Mar 18 08:41:05 2010 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Thu Mar 18 08:41:12 2010 Subject: [Histonet] Chemistry of MSB stain? Message-ID: Hello, Does anyone know something about the chemistry behind the MSB stain, why exactly young fibrin appears yellow and old fibrin bluish? Or where I can find some background info? Thank you very much! Alexandra ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.meinl@trauma.lbg.ac.at From leiker <@t> buffalo.edu Thu Mar 18 09:45:55 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Mar 18 09:46:03 2010 Subject: [Histonet] Anti-human abs work on porcine tissue In-Reply-To: <6CDABB2487DB4002B801FBE12BCFC4AF@auxs.umn.edu> References: <28389.22216.qm@web180613.mail.sp1.yahoo.com> <6CDABB2487DB4002B801FBE12BCFC4AF@auxs.umn.edu> Message-ID: <3CA12DD0FC844ADA5B231CFB@CDYwxp1931.ad.med.buffalo.edu> This is great FYI, Jan. Our lab works a lot with pig tissues, too. And we, too, have found in our lab that a number of human Abs appear to work on pig tissues. Still, I can definitely appreciate the difficulties in finding a pig-specific antibody, dealing the unknowns of cross-reactivity and degree of homology. Jan, I will certainly keep you in mind when we are seaching for a porcine-reacting antibody! Thanks for sharing that. Regards, Merced --On Thursday, March 18, 2010 7:48 AM -0500 Jan Shivers wrote: > I work on all species, porcine being one of them. I've never done a % > survey of the number of Abs made against human epitopes that also work on > porcine tissue, but I can give you generalizations. > > All of the rabbit polyclonal antibodies that I've tried have > cross-reacted with porcine tissue. The polyclonals that I use the most > are the Abs against hormones, neuroendocrine markers, vasculature, > astrocytes, etc. > Most of the mouse monoclonal antibodies that I've tried have > cross-reacted with porcine tissue. These would be the intermediate > filaments, melanoma antigens, cell proliferating antigens, neuroendocrine > markers, etc. > You may have more difficulty with leukocyte markers (CD markers). They > seem to have less homology with human markers than the above items, and > not all cross-react, in my experience. You only learn this by trial and > error, unfortunately, since data about species cross-reactivity is rare. > I have 2 polyclonal and 2 monoclonal CD markers that cross-react with > porcine. That is not to say that there aren't more, but that's all that > I currently have worked up on porcine tissue. > I have not tried Abs like ER, PR, CEA, TTF, etc. > I've done species cross-reactivity studies with every antibody I have in > stock (>80), so if you send me a list of the antibodies you're interested > in (directly to me), I can let you know which ones cross-react with > porcine tissue, and which vendor works for me. > > Jan Shivers > Senior Scientist > Pathology Teaching Program > Histology/IHC/EM Section Head > University of Minnesota > Veterinary Diagnostic Laboratory > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > > (Confidentiality Notice: This message, together with any attachments, is > intended only for the use of the individual or entity to which it is > addressed and may contain confidential or privileged information. If you > think you have received this message in error, please advise the sender > and then delete this message and any attachments immediately.) > > ----- Original Message ----- From: "RICKY MATHIS" > > To: "histonet" > Sent: Wednesday, March 17, 2010 8:15 PM > Subject: [Histonet] Anti-human abs work on porcine tissue > > > I work with some porcine tissues and it is sometimes difficult to find > antibodies specific to pig. A doctor I work with asked some one he knew > in Europe about using antibodies that are listed as working in human > being used on porcine tissue. This person stated that there would be > about a 10% chance that the anti-human antibodies would work on porcine > tissues. The antibody companies mostly give the same "we did not try it > on pig tissue" response. I understand that it is likely expensive to > continue to test antibodies on a wide variety of animal tissues, so that > is fine. But what do you guys think about the 10% chance? I would have > said more than that. > Thank you in advance for your time, > Cathy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From sheila_adey <@t> hotmail.com Thu Mar 18 09:53:58 2010 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Mar 18 09:54:02 2010 Subject: [Histonet] VIP 6 Message-ID: Can anyone give me their opinions on the VIP 6? Thanks in advance Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Live connected with Messenger on your phone http://go.microsoft.com/?linkid=9712958 From llewllew <@t> shaw.ca Thu Mar 18 10:12:14 2010 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Mar 18 10:12:22 2010 Subject: [Histonet] Chemistry of MSB stain? In-Reply-To: References: Message-ID: Read the following pages and the links on them at StainsFile (http://stainsfile.info/StainsFile/ http://stainsfile.info/StainsFile/theory/massact.htm http://stainsfile.info/StainsFile/theory/accent.htm http://stainsfile.info/StainsFile/theory/diff.htm http://stainsfile.info/StainsFile/theory/accent.htm Bryan Llewellyn ----- Original Message ----- From: "Alexandra Meinl" To: "Histonet" Sent: Thursday, March 18, 2010 6:41 AM Subject: [Histonet] Chemistry of MSB stain? > Hello, > > Does anyone know something about the chemistry behind the MSB stain, why > exactly young fibrin appears yellow and old fibrin bluish? > Or where I can find some background info? > > Thank you very much! > > Alexandra > > ************************************************ > Dr. Alexandra Meinl > Ludwig Boltzmann Institute > for Experimental and Clinical Traumatology > Austrian Cluster for Tissue Regeneration > Histology > Donaueschingenstrasse 13 > 1200 Vienna - Austria > > Contact @ Bernhard Gottlieb University School of Dentistry, > Waehringerstr. 25a, A-1090 Vienna > tel: +43 1 4277 67026 > fax: +43 1 4277 67019 > email: alexandra.meinl@trauma.lbg.ac.at > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Sharon.Davis-Devine <@t> carle.com Thu Mar 18 10:30:14 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Thu Mar 18 10:30:52 2010 Subject: [Histonet] Thermo Slide and Cassette printers Message-ID: <50003EC02E2CEA4583BEB3CD08EAC1E090DF15@EXCHANGEBE2.carle.com> Does anyone out there in Histo land have any experience with the new Thermo Scientific PrintMate and SlideMate system? Or do any of you have another system similar to this that you have experience with? We are looking into some of these systems to help reduce errors and make our laboratory more lean. I would appreciate any and all opinions and advice. Thank you. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From llewllew <@t> shaw.ca Thu Mar 18 10:40:22 2010 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Mar 18 10:40:29 2010 Subject: [Histonet] Chemistry of MSB stain? Message-ID: My apologies. The fourth link for the MSB explanation should have been: http://stainsfile.info/StainsFile/theory/tri_gen.htm Bryan Llewellyn From ree3 <@t> leicester.ac.uk Thu Mar 18 10:44:30 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Mar 18 10:44:59 2010 Subject: [Histonet] Mc Coy fixative In-Reply-To: <000001cac5e2$99201da0$cb6058e0$@com> References: <000001cac5e2$99201da0$cb6058e0$@com> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8CFFB6C84@EXC-MBX3.cfs.le.ac.uk> Do you mean the real McCoys??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: 17 March 2010 15:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mc Coy fixative Hi Histonetters I am having a senior moment. Can anyone tell me what McCoy fixative is made of and what it is used for? Thanks in advance for the help. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Thu Mar 18 10:48:12 2010 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Mar 18 10:49:04 2010 Subject: [Histonet] Thermo Slide and Cassette printers In-Reply-To: <50003EC02E2CEA4583BEB3CD08EAC1E090DF15@EXCHANGEBE2.carle.com> References: <50003EC02E2CEA4583BEB3CD08EAC1E090DF15@EXCHANGEBE2.carle.com> Message-ID: <08bf01cac6b2$70f197c0$52d4c740$@com> Hi Sharon, We're an LIS vendor working with a client that just purchased 6 of these. They're 30 days away from go live, but we've played with them for a bit. If you or anyone else is curious about what our experiences have been, feel free to email me. We also trialed cassette labelers from several other companies before our client selected these labelers. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, March 18, 2010 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Slide and Cassette printers Does anyone out there in Histo land have any experience with the new Thermo Scientific PrintMate and SlideMate system? Or do any of you have another system similar to this that you have experience with? We are looking into some of these systems to help reduce errors and make our laboratory more lean. I would appreciate any and all opinions and advice. Thank you. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Mar 18 10:49:15 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Mar 18 10:49:56 2010 Subject: [Histonet] March Madness!!! Brand New Opportunities for Techs, Managers, Supervisors and Pathologist's Assistants Message-ID: Hi Histonetters! I hope everyone had a fun and lucky St. Patricks Day. March Madness has started and in addition to some great College Basketball starting today (Go Gators!) I have been experiencing the madness as well. My phone has literally been ringing off the hook with some great new opportunities. All of these positions are permanent full time positions and my clients offer excellent compensation, benefits and relocation assistance. Best of all these clients are motivated to hire and eager to meet you! Here is a list of my current openings: HISTOLOGY/PATHOLOGY MANAGEMENT AZ ? Phoenix Histology Lab Manager VA ? Richmond Histology Manager NY - Long Island Histology Manager NV ? Histology Supervisor HISTOTECHS TX ? Austin Night Shift Grossing Histotech excellent shift diff TX - Austing Day Shift Histotech TX ? Corpus Christi ? Histotechnician day shift private lab MA ? Cape Cod Immunohistochemistry Specialist MA ? Cape Cod Histotechnologist/Histotechnician GA ? Atlanta area Histotech needed for evening shift GA ? Atlanta area Immunohistochemistry Specialist 2p-10p GA - Atlanta area all shifts - PRN NY-Orange/Rockland County Brand New Lab NYS license/Elig brand new lab 2nd shift. NY - Long Island Mohs - 2 days per week CA-Los Angeles Histotechnologist afternoon shift NV- Las Vegas Histotechnologist HTL and B.S. required PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC ? Charlotte PA grad from NAACLES program required NV- Las Vegas Evening shift If you or anyone you know might be interested in any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net. Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From pruegg <@t> ihctech.net Thu Mar 18 10:28:12 2010 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Thu Mar 18 10:56:17 2010 Subject: [Histonet] CD3 on mouse Message-ID: <3bb0a2e$69d09c4a$6107af14$@com> Joanne, the rabbit polyclonal or rabbit monoclonal cd3's work well on mouse tissue, dako has poly and lab vision i believe has a rab mono, it may be poly too, the key is that it is rabbit and not mouse. ---------------------------------------- From: "Mauger, Joanne" Sent: Wednesday, March 17, 2010 1:34 PM To: "Emily Sours" , "histonet@lists.utsouthwestern.edu" Subject: RE: [Histonet] CD3 on mouse Hi All, Does anyone know a CD3 antibody that works well on FFPE mouse tissue? Thanks in advance, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From burch007 <@t> mc.duke.edu Thu Mar 18 11:03:07 2010 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Thu Mar 18 11:03:25 2010 Subject: [Histonet] CD3 on mouse In-Reply-To: <3bb0a2e$69d09c4a$6107af14$@com> Message-ID: Patsy - I agree. LV's rabbit MoAb works well on mouse tissue. They also have a rabbit polyclonal CD20 that works well on mouse. jb "pruegg@ihctech.net" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2010 11:58 AM Please respond to pruegg@ihctech.net To "Mauger, Joanne" , "Emily Sours" , "histonet@lists.utsouthwestern.edu" cc Subject RE: [Histonet] CD3 on mouse Joanne, the rabbit polyclonal or rabbit monoclonal cd3's work well on mouse tissue, dako has poly and lab vision i believe has a rab mono, it may be poly too, the key is that it is rabbit and not mouse. ---------------------------------------- From: "Mauger, Joanne" Sent: Wednesday, March 17, 2010 1:34 PM To: "Emily Sours" , "histonet@lists.utsouthwestern.edu" Subject: RE: [Histonet] CD3 on mouse Hi All, Does anyone know a CD3 antibody that works well on FFPE mouse tissue? Thanks in advance, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Mar 18 11:10:01 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Mar 18 11:10:05 2010 Subject: [Histonet] Thermo Slide and Cassette printers In-Reply-To: <50003EC02E2CEA4583BEB3CD08EAC1E090DF15@EXCHANGEBE2.carle.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D99@is-e2k3.grhs.net> Me too, please! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, March 18, 2010 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Slide and Cassette printers Does anyone out there in Histo land have any experience with the new Thermo Scientific PrintMate and SlideMate system? Or do any of you have another system similar to this that you have experience with? We are looking into some of these systems to help reduce errors and make our laboratory more lean. I would appreciate any and all opinions and advice. Thank you. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ceri.Allen <@t> nottingham.ac.uk Thu Mar 18 10:35:38 2010 From: Ceri.Allen <@t> nottingham.ac.uk (Ceri Allen) Date: Thu Mar 18 11:13:05 2010 Subject: [Histonet] Removing erythrocytes from fixed tissue Message-ID: <5B721D295F00644C9E1D87C420DB775002B75088@VUIEXCHC.ad.nottingham.ac.uk> Dear Histonetters, Does anyone know whether it is possible to remove erythrocytes from fixed tissue? Background: I am using an IgM antibody with a FITC conjugated secondary on 5 micron sections of formalin fixed tissue. I'm getting a lot of non-specific labelling of erythrocytes, but I'm not sure whether this is due to the IgM binding to the erythrocytes, or auto-fluorescence due to the tissue being formalin fixed. Changing the fixative is not possible as all the experimental tissue was collected a long time ago, So I'm looking for any other solutions. Any suggestions would be gratefully received Ceri Research Technician School of Veterinary Medicine and Science Sutton Bonnington Campus University of Nottingham Tel. 0115 95 16459 ceri.allen@nottingham.ac.uk This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation. From anonwums1 <@t> gmail.com Thu Mar 18 11:32:19 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Mar 18 11:32:23 2010 Subject: [Histonet] Removing erythrocytes from fixed tissue In-Reply-To: <5B721D295F00644C9E1D87C420DB775002B75088@VUIEXCHC.ad.nottingham.ac.uk> References: <5B721D295F00644C9E1D87C420DB775002B75088@VUIEXCHC.ad.nottingham.ac.uk> Message-ID: <858249121003180932p3a53e3d7xaf02ecf0f15b13ae@mail.gmail.com> Autofluorescence from RBCs is a common problem. See the archives for discussion on how to fix this. For example, http://lists.utsouthwestern.edu/pipermail/histonet/2009-December/048210.html Adam On Thu, Mar 18, 2010 at 10:35 AM, Ceri Allen wrote: > Dear Histonetters, > > > > Does anyone know whether it is possible to remove erythrocytes from > fixed tissue? > > > > Background: I am using an IgM antibody with a FITC conjugated secondary > on 5 micron sections of formalin fixed tissue. I'm getting a lot of > non-specific labelling of erythrocytes, but I'm not sure whether this is > due to the IgM binding to the erythrocytes, or auto-fluorescence due to > the tissue being formalin fixed. Changing the fixative is not possible > as all the experimental tissue was collected a long time ago, So I'm > looking for any other solutions. > > > > Any suggestions would be gratefully received > > > > Ceri > > > > Research Technician > > School of Veterinary Medicine and Science > > Sutton Bonnington Campus > > University of Nottingham > > > > Tel. 0115 95 16459 > > ceri.allen@nottingham.ac.uk > > > > This message has been checked for viruses but the contents of an attachment > may still contain software viruses which could damage your computer system: > you are advised to perform your own checks. Email communications with the > University of Nottingham may be monitored as permitted by UK > legislation._______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SARAH.REEVES <@t> ekht.nhs.uk Thu Mar 18 11:29:34 2010 From: SARAH.REEVES <@t> ekht.nhs.uk (SARAH REEVES) Date: Thu Mar 18 11:36:12 2010 Subject: [Histonet] EVG - Temple Artery Message-ID: <4BA254EE020000B500007E3A@ekhtgwia.ekht.nhs.uk> I am looking into EVG staining for temple arteries. Does anyone have any advice or journals on the subject? Thanks in advance Sarah Reeves ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Foundation Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** From jkiernan <@t> uwo.ca Thu Mar 18 11:45:43 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Mar 18 11:45:47 2010 Subject: [Histonet] question about mouse perfusion Message-ID: The volume of a mouse is about 25ml, of which perhaps 2ml is blood. 5ml of saline (or PBS) is enough to wash it all out. Some people precede this with a small bolus (about 0.2ml) of 1% aqueous sodium nitrite to dilate the blood vessels. After washing out the blood, perfuse with about 10 ml of fixative, over the course of 5 to 10 minutes. It's important that the pressure isn't too high, to avoid rupturing capillaries and small veins, and waterlogging (wrecking) the tissues. If perfusing from a syringe, use the thinnest available needle to attenuate the pressure; 10 minutes feels like a lot longer when you're pushing the plunger very slowly and keeping the sharp end of the needle motionless! John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Li Zhang Date: Wednesday, March 17, 2010 14:59 Subject: [Histonet] question about mouse perfusion To: histonet@lists.utsouthwestern.edu > Dear all, > > I have just started to learn how to do perfusion in mice and > this site has helped me a lot. > > We don't have the pump or Perfusion one in the lab, and probably > won't be able to get one in the near future, so I'm using the > hand injection method. I'm using a 60 ml syringe with 21G > needle. I am doing perfusion in order to do immunofluorescence > on the brain. > > My question is: can anyone give me a rough idea of how fast I > should inject ( like ml/min). I think I've tried like 30 ml in 3 > min, and I suspect that it's too fast because I do observe > tissue swelling sometimes. > > And another question, which of the following is better for > perfsusion: 1) PBS and then 4% PFA 2) Saline and then 4% > PFA 3) sucrose--> saline--> PFA? > > Thank you very much for your help! > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MAUGER <@t> email.chop.edu Thu Mar 18 11:50:21 2010 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Thu Mar 18 11:51:52 2010 Subject: [Histonet] RE:Thanks- CD3 on mouse Message-ID: <443F5B475A9BF647AB962E834884EBAD2786DA4E65@EX7CCRPW03V1.chop.edu> Thanks for your very helpful replies- as always. Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: James L Burchette [burch007@mc.duke.edu] Sent: Thursday, March 18, 2010 12:03 PM To: pruegg@ihctech.net Cc: histonet@lists.utsouthwestern.edu; Mauger, Joanne; Emily Sours Subject: RE: [Histonet] CD3 on mouse Patsy - I agree. LV's rabbit MoAb works well on mouse tissue. They also have a rabbit polyclonal CD20 that works well on mouse. jb "pruegg@ihctech.net" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2010 11:58 AM Please respond to pruegg@ihctech.net To "Mauger, Joanne" , "Emily Sours" , "histonet@lists.utsouthwestern.edu" cc Subject RE: [Histonet] CD3 on mouse Joanne, the rabbit polyclonal or rabbit monoclonal cd3's work well on mouse tissue, dako has poly and lab vision i believe has a rab mono, it may be poly too, the key is that it is rabbit and not mouse. ---------------------------------------- From: "Mauger, Joanne" Sent: Wednesday, March 17, 2010 1:34 PM To: "Emily Sours" , "histonet@lists.utsouthwestern.edu" Subject: RE: [Histonet] CD3 on mouse Hi All, Does anyone know a CD3 antibody that works well on FFPE mouse tissue? Thanks in advance, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Thu Mar 18 12:02:20 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Mar 18 12:02:25 2010 Subject: [Histonet] EVG - Temple Artery Message-ID: If EVG = staining for elastin, followed by Van Gieson's picro-fuchsine, try: Silverberg D & Teodorescu V 2005. True aneurysm of the superficial temporal artery. EJVES Extra 9:126-128. The paper includes one coloured micrograph (not very good contrasts) and has 7 references, with two being 4 pages or longer. (EJVES Extra is the "online companion" to the European Journal of Vascular and Endovascular Surgery. Its volumes are numbered differently from the main journal, and the contents are different too.) John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: SARAH REEVES Date: Thursday, March 18, 2010 12:38 Subject: [Histonet] EVG - Temple Artery To: histonet@lists.utsouthwestern.edu > I am looking into EVG staining for temple arteries. Does anyone > have any advice or journals on the subject? > > Thanks in advance > > Sarah Reeves > > > > > ********************************************************************** > This email and any files transmitted with it are confidential > and intended > solely for the use of the individual or entity to whom they are > addressed.Any views or opinions presented are solely those of > the author and do > not necessarily represent those of East Kent Hospitals > University NHS Foundation Trust. If > you are not the intended recipient, be advised that you have received > this email in error and that any use, dissemination, forwarding, > printingor copying of this email is strictly prohibited. > > If you have received this email in error please notify the system > manager at the following email address: root.postmaster@ekht.nhs.uk > > www.kentandmedway.nhs.uk > > This footnote also confirms that although this email message has been > swept by MIMEsweeper for the presence of computer viruses, it is > strongly > recommended that you carry out your own virus scan of this > message and any attachments. > > www.mimesweeper.com > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Thu Mar 18 12:19:54 2010 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Mar 18 12:20:00 2010 Subject: [Histonet] Thermo Slide and Cassette printers In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3D99@is-e2k3.grhs.net> References: <50003EC02E2CEA4583BEB3CD08EAC1E090DF15@EXCHANGEBE2.carle.com> <661949901A768E4F9CC16D8AF8F2838C017A3D99@is-e2k3.grhs.net> Message-ID: me too please - we use LIS Cerner Millennium and I am looking for cassette and slide labellers which can interface - also need to be able to use various slides and various cassettes (not specifically from one manufacturer) AbuDhabiAnnie On 18 March 2010 20:10, Mike Pence wrote: > Me too, please! > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Sharon.Davis-Devine > Sent: Thursday, March 18, 2010 10:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Thermo Slide and Cassette printers > > > Does anyone out there in Histo land have any experience with the new > Thermo Scientific PrintMate and SlideMate system? Or do any of you have > another system similar to this that you have experience with? We are > looking into some of these systems to help reduce errors and make our > laboratory more lean. I would appreciate any and all opinions and > advice. > > > > Thank you. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology-Histology Supervisor > > Carle Foundation Hospital > > Laboratory and Pathology Services > > 611 West Park Street > > Urbana, Illinois 61801 > > 217-383-3572 > > sharon.davis-devine@carle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From Lynne.Bell <@t> cvmc.org Thu Mar 18 12:31:19 2010 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Thu Mar 18 12:31:25 2010 Subject: [Histonet] VIP 6 In-Reply-To: Message-ID: Short and sweet - the best processor available. Love mine ?! Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From making <@t> ufl.edu Thu Mar 18 12:32:11 2010 From: making <@t> ufl.edu (MKing) Date: Thu Mar 18 12:33:57 2010 Subject: [Histonet] mouse perfusion rate Message-ID: <4BA2639B.6080600@ufl.edu> Li, Mouse cardiac output seems to be about 17 ml/min (e.g. www.transonic.com/mice1.shtml), you probably want to try for that to keep pressures close to physiological. A syringe pump is pretty inexpensive and probably all you need. Mike ----- Original Message ----- From: Li Zhang Date: Wednesday, March 17, 2010 14:59 Subject: [Histonet] question about mouse perfusion To: histonet@lists.utsouthwestern.edu > > My question is: can anyone give me a rough idea of how fast I > > should inject ( like ml/min). I think I've tried like 30 ml in 3 > > min, and I suspect that it's too fast because I do observe > > tissue swelling sometimes. From leiker <@t> buffalo.edu Thu Mar 18 12:37:49 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Mar 18 12:38:03 2010 Subject: [Histonet] mouse perfusion rate In-Reply-To: <4BA2639B.6080600@ufl.edu> References: <4BA2639B.6080600@ufl.edu> Message-ID: <20755543F732580ADB775B92@CDYwxp1931.ad.med.buffalo.edu> That may be mouse cardiac output, but I can assure you, from experience, you do not want to perfuse at 17ml/min. Regards, Merced --On Thursday, March 18, 2010 1:32 PM -0400 MKing wrote: > Li, > > Mouse cardiac output seems to be about 17 ml/min (e.g. > www.transonic.com/mice1.shtml), you probably want to try for that to keep > pressures close to physiological. > A syringe pump is pretty inexpensive and probably all you need. > > Mike > > ----- Original Message ----- > From: Li Zhang > Date: Wednesday, March 17, 2010 14:59 > Subject: [Histonet] question about mouse perfusion > To: histonet@lists.utsouthwestern.edu > > > > My question is: can anyone give me a rough idea of how fast I > > > should inject ( like ml/min). I think I've tried like 30 ml in 3 > > > min, and I suspect that it's too fast because I do observe > > > tissue swelling sometimes. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From JEllin <@t> yumaregional.org Thu Mar 18 13:12:15 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Mar 18 13:12:25 2010 Subject: [Histonet] Thermo Slide and Cassette printers In-Reply-To: <08bf01cac6b2$70f197c0$52d4c740$@com> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C613B@EXCHANGECLUSTER.yumaregional.local> We have the Printmate coming on board and have used the slide mate. The Printmate is excellent, the slide mate is good but if you have it connected to LIS there is lag time. Also take into consideration that the barcodes have to be read and that traditional scrapping does hinder this. You will need to look at getting a hot plate for the blocks so that the read is clear, clean and concise. Also the color will play in issue. AS mike said there are different vendors out there, but for me for space and accuracy, since every histology labs has space, I would go with looking at thermo or General Data. If you have any questions feel free to give me a call. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center 928-336-1743 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Thursday, March 18, 2010 8:48 AM To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo Slide and Cassette printers Hi Sharon, We're an LIS vendor working with a client that just purchased 6 of these. They're 30 days away from go live, but we've played with them for a bit. If you or anyone else is curious about what our experiences have been, feel free to email me. We also trialed cassette labelers from several other companies before our client selected these labelers. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, March 18, 2010 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Slide and Cassette printers Does anyone out there in Histo land have any experience with the new Thermo Scientific PrintMate and SlideMate system? Or do any of you have another system similar to this that you have experience with? We are looking into some of these systems to help reduce errors and make our laboratory more lean. I would appreciate any and all opinions and advice. Thank you. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From vavalos <@t> allergydermatology.com Thu Mar 18 13:17:05 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Thu Mar 18 13:17:11 2010 Subject: [Histonet] slide template Message-ID: <000a01cac6c7$378a4300$a69ec900$@com> Would anyone have a template for a ? sheet of 15/16 x 15/16 microscope slide labels? Creating templates is not something I am very good at. Fax:602-277-2134 From sparker <@t> vt.edu Thu Mar 18 13:54:42 2010 From: sparker <@t> vt.edu (Scott Parker) Date: Thu Mar 18 13:54:48 2010 Subject: [Histonet] Spencer 820 Microtome Thank you Message-ID: My most heartfelt thanks to all those who answered my call for help. Collectively you provided me with very helpful information that allowed me to reach a decision regarding my microtome. Best regards to all, Scott Scott L. Parker, PhD Assistant Professor Biology Coastal Carolina University P.O. Box 261954 Conway, SC 29528-6054 From mike <@t> pathview.com Thu Mar 18 14:19:59 2010 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Mar 18 14:20:51 2010 Subject: [Histonet] Thermo Slide and Cassette printers In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8021C613B@EXCHANGECLUSTER.yumaregional.local> References: <08bf01cac6b2$70f197c0$52d4c740$@com> <29BE166A2CF48D459853F8EC57CD37E8021C613B@EXCHANGECLUSTER.yumaregional.local> Message-ID: <091a01cac6d0$07b77040$172650c0$@com> Guys, I'll be happy to respond, but I'm in the middle of some things now and I'd like to spend some time on my response. However, my quick response is EXACTLY what Jesus is saying: GD or Thermo and lighter/pastel colors seem to read easier. The hot plate is one of the things they also concur with, but still, don't press too hard on the hot plate or the bar code might be affected. ...more later, and I'll just respond to the group of people who queried me. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Thursday, March 18, 2010 1:12 PM To: Michael Mihalik; Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo Slide and Cassette printers We have the Printmate coming on board and have used the slide mate. The Printmate is excellent, the slide mate is good but if you have it connected to LIS there is lag time. Also take into consideration that the barcodes have to be read and that traditional scrapping does hinder this. You will need to look at getting a hot plate for the blocks so that the read is clear, clean and concise. Also the color will play in issue. AS mike said there are different vendors out there, but for me for space and accuracy, since every histology labs has space, I would go with looking at thermo or General Data. If you have any questions feel free to give me a call. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center 928-336-1743 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Thursday, March 18, 2010 8:48 AM To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo Slide and Cassette printers Hi Sharon, We're an LIS vendor working with a client that just purchased 6 of these. They're 30 days away from go live, but we've played with them for a bit. If you or anyone else is curious about what our experiences have been, feel free to email me. We also trialed cassette labelers from several other companies before our client selected these labelers. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, March 18, 2010 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Slide and Cassette printers Does anyone out there in Histo land have any experience with the new Thermo Scientific PrintMate and SlideMate system? Or do any of you have another system similar to this that you have experience with? We are looking into some of these systems to help reduce errors and make our laboratory more lean. I would appreciate any and all opinions and advice. Thank you. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From arvidsonkristen <@t> yahoo.com Thu Mar 18 14:22:33 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Thu Mar 18 14:22:38 2010 Subject: [Histonet] Dermpath Lab People Message-ID: <228844.91466.qm@web65705.mail.ac4.yahoo.com> Hello, I am looking for a network of people who would be willing to share info on derm stuff.? I am currently the supervisor of a fairly large derm lab.? We've been open about 13 yrs and I've worked here about 12 yrs (Sup for about 1).? Things are going pretty well but when I run into problems I would like to share my info with people who may have similar experiences.? Currently, I am having some difficulty with quality.? Tissues seem a little dry and tough, but they act sensitive to handling.? Upon microscopic exam they ofter look torn.? The tearing seems to be occurring a lot at the dermal/epidermal junction.? At first I thought it may be happening at embedding but now I not so sure.? Our processing schedule/reagents haven't changed and peoples work habits are the same.? The Paths have noticed the problem for about 2 months.? Any help would be greatly appreciated.? I hope I can be of service to one of you in the future. ? Kristen From NMargaryan <@t> childrensmemorial.org Thu Mar 18 14:38:33 2010 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Mar 18 14:52:03 2010 Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer In-Reply-To: References: Message-ID: Hi Histonetters! I have a stupid question but I Have to ask. Does anyone perform an Incubation of Ab that required more then 1 hour (2-3 hours) in autostainer? Does autostainer keep slides wet or slides sometimes are getting dry? I know that slides should not be dry in any step of IHC. Thank you very much! Naira From stamptrain <@t> yahoo.com Thu Mar 18 15:02:17 2010 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Thu Mar 18 15:02:21 2010 Subject: [Histonet] slide template In-Reply-To: <000a01cac6c7$378a4300$a69ec900$@com> References: <000a01cac6c7$378a4300$a69ec900$@com> Message-ID: <38185.17563.qm@web55807.mail.re3.yahoo.com> Actually it's very easy if you have Microsoft Word and the Avery plug-in (assuming this is an Avery sheet of labels).? There is a wizard that will guide you through the process.? I am similarly "template-challenged" yet find it fairly easy to use the Avery wizard in Word.? Don't know if this is available for other word processors (e.g. the free OpenOffice) but wouldn't be surprised. Roger Moretz, Ph.D., (ret.) ----- Original Message ---- From: Vanessa Avalos To: HISTONET LISTS Sent: Thu, March 18, 2010 2:17:05 PM Subject: [Histonet] slide template Would anyone have a template for a ? sheet of 15/16 x 15/16 microscope slide labels?? Creating templates is not something I am very good at. Fax:602-277-2134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Mar 18 15:04:47 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Mar 18 15:04:50 2010 Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer In-Reply-To: Message-ID: We have done up to an hour but not longer, if I were you I would have two antibody steps and just to add some more reagent after an hour or 1.5 hours Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, March 18, 2010 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer Importance: High Hi Histonetters! I have a stupid question but I Have to ask. Does anyone perform an Incubation of Ab that required more then 1 hour (2-3 hours) in autostainer? Does autostainer keep slides wet or slides sometimes are getting dry? I know that slides should not be dry in any step of IHC. Thank you very much! Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu Mar 18 15:43:32 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Mar 18 15:43:37 2010 Subject: [Histonet] Cost per slide? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46F8C@nmdamailsvr.nmda.ad.nmsu.edu> Salutations (that's my real name) to all of you in the VETERINARY DIAGNOSTIC world. I need to come up with the cost of producing one H&E slide and because I'm Old and Lazy (not), I'm wondering if any of you have already done this complicated calculation and have a number I could use. I barely have enough time to gross in, type reports, embed, cut, stain, coverslip, file slides/blocks and do special stains and immunos as it is! I shouldn't claim I'm "lazy" but why invent the wheel twice, I'm sayin'. I'd appreciate any cost you might have... Muchas gracias! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From STACEY.LANGENBERG <@t> UCDENVER.EDU Thu Mar 18 16:50:44 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Thu Mar 18 16:53:15 2010 Subject: [Histonet] Thermo Slide and Cassette printers In-Reply-To: <50003EC02E2CEA4583BEB3CD08EAC1E090DF15@EXCHANGEBE2.carle.com> References: <50003EC02E2CEA4583BEB3CD08EAC1E090DF15@EXCHANGEBE2.carle.com> Message-ID: <483344102.807987.1268949058270.JavaMail.rim@bda2340.bisx.prod.on.blackberry> Sharon We have both the Leica slide and cassette printer. We like both of them very much and have virtually error proofed our slide making process. Stacey Langenberg CU Dermatopathology Consultants Sent via BlackBerry from T-Mobile -----Original Message----- From: Sharon.Davis-Devine Date: Thu, 18 Mar 2010 09:30:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Slide and Cassette printers Does anyone out there in Histo land have any experience with the new Thermo Scientific PrintMate and SlideMate system? Or do any of you have another system similar to this that you have experience with? We are looking into some of these systems to help reduce errors and make our laboratory more lean. I would appreciate any and all opinions and advice. Thank you. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Fri Mar 19 06:56:19 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Mar 19 06:56:22 2010 Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer In-Reply-To: References: Message-ID: <362635.77993.qm@web50304.mail.re2.yahoo.com> Hi Naira, We routinely do a 2 hour incubation in primary, but we make sure that more antibody is on the slide than would be necessary for most other reagents (500-600 ul).? We have not had any issues of drying.? If you are concerned about it, just make it 2 antibody steps instead of 1 on the machine. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Margaryan, Naira" To: "histonet@lists.utsouthwestern.edu" Sent: Thu, March 18, 2010 3:38:33 PM Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer Hi Histonetters! I have a stupid question but I Have to ask. Does anyone perform an Incubation of Ab that required more then 1 hour (2-3 hours) in autostainer? Does autostainer keep slides wet or slides sometimes are getting dry? I know that slides should not be dry in any step of IHC. Thank you very much! Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Fri Mar 19 07:57:29 2010 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Mar 19 07:57:38 2010 Subject: [Histonet] Processor malfunction Message-ID: <3809C163DC1DA54AA534B3C7794D07B652EE426E61@ENHBGMBX01.PA.LCL> Happy Friday to all, Our processor shut off last evening in 80% alcohol due to a misconnection of the next reagent. We restarted this morning when we arrived but the tissues were sitting in 80% for 9 hours. My question is what affect will this have on the tissues which are a mixture of medulla and lymph node sections? Thanks roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 From Barry.R.Rittman <@t> uth.tmc.edu Fri Mar 19 08:05:00 2010 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Mar 19 08:08:41 2010 Subject: [Histonet] RE: Processor malfunction In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B652EE426E61@ENHBGMBX01.PA.LCL> References: <3809C163DC1DA54AA534B3C7794D07B652EE426E61@ENHBGMBX01.PA.LCL> Message-ID: <75A0543E23D3A7458012D9E02EDBEC00098E3D7E45@UTHCMS1.uthouston.edu> Roger The tissue may be sightly harder and a little more difficult therefore to section but apart from that don't see any real problems. You may not notice any difference. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger [rcharles@state.pa.us] Sent: Friday, March 19, 2010 7:57 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Processor malfunction Happy Friday to all, Our processor shut off last evening in 80% alcohol due to a misconnection of the next reagent. We restarted this morning when we arrived but the tissues were sitting in 80% for 9 hours. My question is what affect will this have on the tissues which are a mixture of medulla and lymph node sections? Thanks roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Fri Mar 19 08:21:38 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Fri Mar 19 08:21:43 2010 Subject: [Histonet] mouse perfusion rate In-Reply-To: References: Message-ID: <8A369CED96A71BED89D360E5@CDYwxp1931.ad.med.buffalo.edu> The vasculature will leak too much and the mouse will get bloated - you'll see it first in either the intestines blowing up like a balloon or fluid coming out of the nose. Just not the same as the heart pumping when the mouse is alive with intact physiology and normal functioning. Don't know exactly why, but that's what happens when you go too fast. Perhaps the vasculature has lost its control to compensate for the pressure? I'm not a physiologist so I'm not sure why...maybe someone on the Histonet can answer that? Regards, Merced --On Thursday, March 18, 2010 5:49 PM -0500 Charles.Scouten@leica-microsystems.com wrote: > > > Why not? What happens? One would think the mammalian cardiovascular > system could withstand physiological pressures and flow rates, at least > for one lifetime? > > > > > Cordially, > > Charles W. Scouten, Ph.D > > Product Manager, MNL > > Biosystems Division > > > > Leica Biosystems Richmond, Inc. > 5205 Route 12 > P.O. Box 528 > Richmond, IL 60071 > United States of America > > Telephone 630 964 0501 > > facsimile +1 630 964 0576 > > www.MyNeuroLab.com > > www.leica-microsystems.com > > > > IMPORTANT - This email and any attachments may be confidential. Any > retransmissions, dissemination or other use of > > these materials by persons or entities other than the intended recipient > is prohibited. If received in error, please contact > > us and delete all copies. Before opening or using attachments, check them > for viruses and defects. Our liability is limited > > to resupplying any affected attachments. [Any representations or opinions > expressed in this email are those of the > > individual sender]. > > > > > > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M > Leiker > Sent: Thursday, March 18, 2010 12:38 PM > To: MKing ; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] mouse perfusion rate > > > > That may be mouse cardiac output, but I can assure you, from experience, > you do not want to perfuse at 17ml/min. > > Regards, > Merced > > --On Thursday, March 18, 2010 1:32 PM -0400 MKing < making@ufl.edu> > wrote: > >> Li, >> >> Mouse cardiac output seems to be about 17 ml/min (e.g. >> www.transonic.com/mice1.shtml), you probably want to try for that to >> keep pressures close to physiological. >> A syringe pump is pretty inexpensive and probably all you need. >> >> Mike >> >> ----- Original Message ----- >> From: Li Zhang < dancingwing@yahoo.com> >> Date: Wednesday, March 17, 2010 14:59 >> Subject: [Histonet] question about mouse perfusion >> To: histonet@lists.utsouthwestern.edu >> >> > > My question is: can anyone give me a rough idea of how fast I >> > > should inject ( like ml/min). I think I've tried like 30 ml in 3 >> > > min, and I suspect that it's too fast because I do observe >> > > tissue swelling sometimes. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From pruegg <@t> ihctech.net Fri Mar 19 09:02:20 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Mar 19 09:03:21 2010 Subject: SPAM-LOW: RE: [Histonet] Incubation of Ab more then 1 hour in autostainer In-Reply-To: References: Message-ID: Yea I don't do incubations for longer than one hour on the autostainer and I do put thin trays of water under the slides and on the sides of the machine (it is very dry in Colorado), but I can think of a way you could do longer incubations if you were around, put a coverslip or parafilm over the section after the ab has been applied, you would just have to be there to take it off before the machine goes on to the next steps. This is one area the Leica Bond with coverplates and the Ventana with liq coverslip has over the Dako. I do 2 hour hybridization at 37dc for ish on the bond with the coverplates and the sections do not dry out. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, March 18, 2010 2:05 PM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] Incubation of Ab more then 1 hour in autostainer We have done up to an hour but not longer, if I were you I would have two antibody steps and just to add some more reagent after an hour or 1.5 hours Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, March 18, 2010 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer Importance: High Hi Histonetters! I have a stupid question but I Have to ask. Does anyone perform an Incubation of Ab that required more then 1 hour (2-3 hours) in autostainer? Does autostainer keep slides wet or slides sometimes are getting dry? I know that slides should not be dry in any step of IHC. Thank you very much! Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Traczyk7 <@t> aol.com Fri Mar 19 09:09:01 2010 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Fri Mar 19 09:09:24 2010 Subject: [Histonet] Dermpath: dermal/epidermal junction Message-ID: Kristen, Have you noticed the artifact in every section of a ribbon, or are some sections better than others? If it's in all or most of the ribbon then you might want to look at the temperature of, or how much time the paraffin ribbon stays on the waterbath. It may not be tearing of the dermal/epidermal junction but separation due to heat. If it's only in a few sections, then a mechanical reason for the separation could be that the techs are using forceps to remove wrinkles or folds and inadvertently making tears in the sections. Good luck and let us know if you solve the problem. Dorothy Dorothy Traczyk MTA Histology LLC dorothy@mtahistology.com In a message dated 3/18/2010 3:23:48 P.M. Eastern Daylight Time, arvidsonkristen@yahoo.com writes: Hello, I am looking for a network of people who would be willing to share info on derm stuff. I am currently the supervisor of a fairly large derm lab. We've been open about 13 yrs and I've worked here about 12 yrs (Sup for about 1). Things are going pretty well but when I run into problems I would like to share my info with people who may have similar experiences. Currently, I am having some difficulty with quality. Tissues seem a little dry and tough, but they act sensitive to handling. Upon microscopic exam they ofter look torn. The tearing seems to be occurring a lot at the dermal/epidermal junction. At first I thought it may be happening at embedding but now I not so sure. Our processing schedule/reagents haven't changed and peoples work habits are the same. The Paths have noticed the problem for about 2 months. Any help would be greatly appreciated. I hope I can be of service to one of you in the future. Kristen From NMargaryan <@t> childrensmemorial.org Fri Mar 19 09:22:05 2010 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Mar 19 09:21:48 2010 Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer Message-ID: Dear Histonetters, Thanks everyone for several good advices. I'll try all suggestions: increase volume from 600ul to 800ul, add more primary antibody step and put hot water dishes inside of machine to keep humidity as high as possible. Hopefully it will work........... Many thanks, Naira -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Thursday, March 18, 2010 3:05 PM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Incubation of Ab more then 1 hour in autostainer We have done up to an hour but not longer, if I were you I would have two antibody steps and just to add some more reagent after an hour or 1.5 hours Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, March 18, 2010 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer Importance: High Hi Histonetters! I have a stupid question but I Have to ask. Does anyone perform an Incubation of Ab that required more then 1 hour (2-3 hours) in autostainer? Does autostainer keep slides wet or slides sometimes are getting dry? I know that slides should not be dry in any step of IHC. Thank you very much! Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kldgal1 <@t> yahoo.com Fri Mar 19 09:34:52 2010 From: kldgal1 <@t> yahoo.com (Kelley Durden) Date: Fri Mar 19 09:34:58 2010 Subject: [Histonet] H pylori by IHC Message-ID: <14984.86784.qm@web112907.mail.gq1.yahoo.com> Does anyone have a recommendation for learning IHC for H pylori? ? Nice product? Nice protocol? Nice learning tool? From sbreeden <@t> nmda.nmsu.edu Fri Mar 19 09:41:48 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Mar 19 09:41:53 2010 Subject: [Histonet] Slide Costing Thanks Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46F99@nmdamailsvr.nmda.ad.nmsu.edu> Thanks to those of you who sent me Magical Spreadsheets for Calculating Histo Costs. I highly recommend the one that Tim Morken did for a presentation at 2007 NSH - it is excellent and covers every conceivable scenario and allows you to calculate anything. I hesitate to forward it without his permission, but I'm certain you could contact him directly and ask for a copy. It's priceless (as in "worth the price of admission"). Thank you to all. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Timothy.Morken <@t> ucsfmedctr.org Fri Mar 19 10:15:17 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Mar 19 10:15:28 2010 Subject: [Histonet] RE: Slide Costing Thanks In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46F99@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46F99@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1AAF670737F193429070841C6B2ADD4C013AEB9426@EXMBMCB15.ucsfmedicalcenter.org> Thanks Sara, I'm glad you find it useful. I give out the spreadsheet to anyone who wants it and you can pass it on to whoever wants it. It was part of several NSH workshops I and Jan Gardner gave on cost accounting so those who attended those workshops received a copy of it as well. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, March 19, 2010 7:42 AM To: histonet Subject: [Histonet] Slide Costing Thanks Thanks to those of you who sent me Magical Spreadsheets for Calculating Histo Costs. I highly recommend the one that Tim Morken did for a presentation at 2007 NSH - it is excellent and covers every conceivable scenario and allows you to calculate anything. I hesitate to forward it without his permission, but I'm certain you could contact him directly and ask for a copy. It's priceless (as in "worth the price of admission"). Thank you to all. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Mar 19 10:19:33 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Mar 19 10:19:38 2010 Subject: [Histonet] H pylori by IHC In-Reply-To: <14984.86784.qm@web112907.mail.gq1.yahoo.com> Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF7ED@UWHC-MAIL01.uwhis.hosp.wisc.edu> Check the archives, I recently responded to Histonet on this very topic. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelley Durden Sent: Friday, March 19, 2010 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H pylori by IHC Does anyone have a recommendation for learning IHC for H pylori? ? Nice product? Nice protocol? Nice learning tool? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Brenda <@t> nsh.org Fri Mar 19 10:56:31 2010 From: Brenda <@t> nsh.org (Brenda Royce) Date: Fri Mar 19 10:56:38 2010 Subject: [Histonet] NSH Summer Symposium and VIR Forum Message-ID: The 3rd Annual NSH Summer Symposium will be held in Indianapolis, IN this year on June 14-15, 2010. Be the leader in the histo-race and come to Indy to get the education and tools needed to stay ahead. You can earn up to 10 continuing education hours during this 2 day event. On-line registration is now open. Please visit our website www.nsh.org to view program and reserve your spot today! The NSH VIR Forum also has a few spaces left. This One-Day Forum is next weekend (March 27, 2010) in Bethesda, MD. Visit website or call the office for more information. www.nsh.org or call NSH office at 443-535-4060. . From Lise.Matzke <@t> hli.ubc.ca Fri Mar 19 11:09:46 2010 From: Lise.Matzke <@t> hli.ubc.ca (Lise Matzke) Date: Fri Mar 19 11:10:15 2010 Subject: [Histonet] Histologic image of fibromuscular dysplasia (FMD) Message-ID: <4BA33F59.F029.0016.1@hli.ubc.ca> Hi there, I'm wondering if anyone has a histologic image of an artery from a case of fibromuscular dysplasia (FMD) that I could borrow. thanks, Lise ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From JWeems <@t> sjha.org Fri Mar 19 11:53:43 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Mar 19 11:53:49 2010 Subject: [Histonet] Fungus Controls Message-ID: <27648A6C5BC9B145813DF5182F83AB45075A4E@ITSSSXM01V1.one.ads.che.org> I have requested fungus control from the NSH bank, and now I'm asking you guys... do you have non-Aspergillus controls? Our pathologists do not like us to use Aspergillus and we are looking for Histoplasmosis if anyone would have any to share. Thanks and Happy Friday! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From sjchtascp <@t> yahoo.com Fri Mar 19 12:01:44 2010 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Mar 19 12:01:47 2010 Subject: [Histonet] HT Text Wanted Message-ID: <373145.21590.qm@web38207.mail.mud.yahoo.com> Does anyone have a used HT text I can purchase.? I'm gettiing ready to re-enter the job force and need to study up. Thanks, Steve? sjchtascp@yahoo.com From ekronenberger <@t> sbcglobal.net Fri Mar 19 12:27:41 2010 From: ekronenberger <@t> sbcglobal.net (Elizabeth Kronenberger) Date: Fri Mar 19 12:28:35 2010 Subject: [Histonet] Histology Supervisor Job Opening Message-ID: <8E890D89A8A046E882E714BC6FA799F3@dlne.local> We are a thriving dermatopathology laboratory in operation for 15 years, with 4 pathologists and 6 histology technicians, processing 25,000 biopsies/year. The supervisor oversees all laboratory functions, with the primary role being participation with and supervision of our excellent team of histotechs in all aspects of processing skin biopsies, including grossing, embedding, cutting and special staining. Possible opportunity for direct patient care as well. Experience with skin biopsies and immunoperoxidase staining strongly desirable. HT or HTL certification and strong interpersonal skills a must. Pleasant work environment and nice setting, situated in central Connecticut with easy access from major highways. Excellent pay and benefits including full medical and 401(k) profit sharing plan. Please send resume to: DERMATOPATHOLOGY LABORATORY OF NEW ENGLAND, P.C. Attn: Liz Kronenberger 140 Green Road . Meriden, CT 06450 Fax: (203) 630-2909 . Tel: (203) 630-2666 ekronenberger@sbcglobal.net From lyork <@t> kwbpathology.com Fri Mar 19 14:34:23 2010 From: lyork <@t> kwbpathology.com (LYork) Date: Fri Mar 19 14:34:51 2010 Subject: [Histonet] Florida Histotech needed Message-ID: <000601cac79b$31177680$8e01a8c0@KWBPA.local> Panama City, Florida seeking experienced Histotechnician for new lab in dermatology practice. Responsibilities include grossing, processing, embedding, cutting, staining, and cover-slipping of skin specimens. HT(ASCP) certification preferred, no Florida license required. Lab is located on the white sandy beaches of Florida in Panama City. Position includes excellent compensation and benefits. Drug and criminal background screening are required. For more information contact Shirley Schroyer at: shirleysgulfcoastdermatology@yahoo.com or (850) 233-3376 ext. 1028. ************************************************************************************ This footnote confirms that this email message has been scanned by PineApp Mail-SeCure for the presence of malicious code, vandals & computer viruses. ************************************************************************************ From sweething63 <@t> msn.com Fri Mar 19 15:42:26 2010 From: sweething63 <@t> msn.com (R J VAZQUEZ) Date: Fri Mar 19 15:42:31 2010 Subject: [Histonet] Dermpath Lab People In-Reply-To: <228844.91466.qm@web65705.mail.ac4.yahoo.com> References: <228844.91466.qm@web65705.mail.ac4.yahoo.com> Message-ID: Kristen Are the tissues coming from the same client or is it all derms across the board and just some of them? To me if the tissue is separating at the dermal/epidermal junction, that saline or some sort of salt solution is involved. Just a thought. Robyn > Date: Thu, 18 Mar 2010 12:22:33 -0700 > From: arvidsonkristen@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Dermpath Lab People > > Hello, > I am looking for a network of people who would be willing to share info on derm stuff. I am currently the supervisor of a fairly large derm lab. We've been open about 13 yrs and I've worked here about 12 yrs (Sup for about 1). Things are going pretty well but when I run into problems I would like to share my info with people who may have similar experiences. Currently, I am having some difficulty with quality. Tissues seem a little dry and tough, but they act sensitive to handling. Upon microscopic exam they ofter look torn. The tearing seems to be occurring a lot at the dermal/epidermal junction. At first I thought it may be happening at embedding but now I not so sure. Our processing schedule/reagents haven't changed and peoples work habits are the same. The Paths have noticed the problem for about 2 months. Any help would be greatly appreciated. I hope I can be of service to one of you in the future. > > Kristen > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From clqsousa <@t> gmail.com Fri Mar 19 16:05:00 2010 From: clqsousa <@t> gmail.com (Carol Bain) Date: Fri Mar 19 16:05:06 2010 Subject: [Histonet] Hart Tissue Online Group is born! Message-ID: <84bfdcb01003191405y3d4cb989q14c019b134c01fbb@mail.gmail.com> The hardtissue group at Yahoo! Groups is good to go. Here are the details on hardtissue: Group home page: http://groups.yahoo.com/group/hardtissue Group email address: hardtissue@yahoogroups.com Ready to start? Send an e-mail to my gmail account - clqsousa - and I will add you as a member. Then, get the ball rolling by posting group messages. We can add photos, have an event calendar, share files, create polls, have a database, etc. Make yourself at home in our new group. Simply drop by the hardtissue homepage now. Hope to see you there! Carol From JMacDonald <@t> mtsac.edu Fri Mar 19 16:57:35 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Mar 19 16:57:41 2010 Subject: [Histonet] Edge-Rite blades Message-ID: Does anyone out there have a current supplier for Edge-Rite blades? Thank you, Jennifer MacDonald From Erik.Dokken <@t> onassignment.com Fri Mar 19 18:05:27 2010 From: Erik.Dokken <@t> onassignment.com (Erik Dokken) Date: Fri Mar 19 18:09:05 2010 Subject: [Histonet] Histotech Needed in SF Bay Area. In-Reply-To: <201003191703.o2JH2Vi0030778@smtp11.onasgn.net> Message-ID: On Assignment Healthcare is currently looking for a Histo Tech for a contract assignment here in the Bay Area. This assignment would start ASAP and will be for an indefinite period of time as it is to covering for a leave of absence. Hours are: Monday through Friday, 5:30am to 2:00pm. For additional information please contact me via email. Erik Dokken Market Leader - Northwest Region On Assignment, Inc. Local Allied Healthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, March 19, 2010 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Slide Costing Thanks (Breeden, Sara) 2. RE: Slide Costing Thanks (Morken, Tim) 3. RE: H pylori by IHC (Sebree Linda A) 4. NSH Summer Symposium and VIR Forum (Brenda Royce) 5. Histologic image of fibromuscular dysplasia (FMD) (Lise Matzke) 6. Fungus Controls (Weems, Joyce) ---------------------------------------------------------------------- Message: 1 Date: Fri, 19 Mar 2010 08:41:48 -0600 From: "Breeden, Sara" Subject: [Histonet] Slide Costing Thanks To: "histonet" Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46F99@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Thanks to those of you who sent me Magical Spreadsheets for Calculating Histo Costs. I highly recommend the one that Tim Morken did for a presentation at 2007 NSH - it is excellent and covers every conceivable scenario and allows you to calculate anything. I hesitate to forward it without his permission, but I'm certain you could contact him directly and ask for a copy. It's priceless (as in "worth the price of admission"). Thank you to all. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 2 Date: Fri, 19 Mar 2010 07:15:17 -0800 From: "Morken, Tim" Subject: [Histonet] RE: Slide Costing Thanks To: "Breeden, Sara" , histonet Message-ID: <1AAF670737F193429070841C6B2ADD4C013AEB9426@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Thanks Sara, I'm glad you find it useful. I give out the spreadsheet to anyone who wants it and you can pass it on to whoever wants it. It was part of several NSH workshops I and Jan Gardner gave on cost accounting so those who attended those workshops received a copy of it as well. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, March 19, 2010 7:42 AM To: histonet Subject: [Histonet] Slide Costing Thanks Thanks to those of you who sent me Magical Spreadsheets for Calculating Histo Costs. I highly recommend the one that Tim Morken did for a presentation at 2007 NSH - it is excellent and covers every conceivable scenario and allows you to calculate anything. I hesitate to forward it without his permission, but I'm certain you could contact him directly and ask for a copy. It's priceless (as in "worth the price of admission"). Thank you to all. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 19 Mar 2010 10:19:33 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] H pylori by IHC To: "Kelley Durden" , Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF7ED@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" Check the archives, I recently responded to Histonet on this very topic. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelley Durden Sent: Friday, March 19, 2010 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H pylori by IHC Does anyone have a recommendation for learning IHC for H pylori? ? Nice product? Nice protocol? Nice learning tool? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 19 Mar 2010 11:56:31 -0400 From: "Brenda Royce" Subject: [Histonet] NSH Summer Symposium and VIR Forum To: Message-ID: Content-Type: text/plain; charset="us-ascii" The 3rd Annual NSH Summer Symposium will be held in Indianapolis, IN this year on June 14-15, 2010. Be the leader in the histo-race and come to Indy to get the education and tools needed to stay ahead. You can earn up to 10 continuing education hours during this 2 day event. On-line registration is now open. Please visit our website www.nsh.org to view program and reserve your spot today! The NSH VIR Forum also has a few spaces left. This One-Day Forum is next weekend (March 27, 2010) in Bethesda, MD. Visit website or call the office for more information. www.nsh.org or call NSH office at 443-535-4060. . ------------------------------ Message: 5 Date: Fri, 19 Mar 2010 09:09:46 -0700 From: "Lise Matzke" Subject: [Histonet] Histologic image of fibromuscular dysplasia (FMD) To: Message-ID: <4BA33F59.F029.0016.1@hli.ubc.ca> Content-Type: text/plain; charset=US-ASCII Hi there, I'm wondering if anyone has a histologic image of an artery from a case of fibromuscular dysplasia (FMD) that I could borrow. thanks, Lise ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ------------------------------ Message: 6 Date: Fri, 19 Mar 2010 12:53:43 -0400 From: "Weems, Joyce" Subject: [Histonet] Fungus Controls To: "Histonet@lists.utsouthwestern.edu" Message-ID: <27648A6C5BC9B145813DF5182F83AB45075A4E@ITSSSXM01V1.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I have requested fungus control from the NSH bank, and now I'm asking you guys... do you have non-Aspergillus controls? Our pathologists do not like us to use Aspergillus and we are looking for Histoplasmosis if anyone would have any to share. Thanks and Happy Friday! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 29 **************************************** From greenjumpyone <@t> hotmail.com Fri Mar 19 19:06:13 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Fri Mar 19 19:06:17 2010 Subject: [Histonet] (no subject) Message-ID: http://www.bmwci.yaad.net/6kt1xp9zdw.html _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850553/direct/01/ From clqsousa <@t> gmail.com Fri Mar 19 22:53:14 2010 From: clqsousa <@t> gmail.com (Carol Bain) Date: Fri Mar 19 22:53:23 2010 Subject: [Histonet] HarD Tissue Online Group is born! Message-ID: <84bfdcb01003192053i7d5fa19bs3c2961a4ddb178ff@mail.gmail.com> I can't believe I mispelled "hard tissue" on the subject of my first message! Sorry about that! I have sent an invite to all of you who responded. Thank you all! Have a great weekend, CB p.s.: Generally speaking this is a new e-group independent from histonet, dedicated to connect people who work with hard tissue as bone, tissue with biomaterials as stented arteries, embedded either in resins or paraffin (decal bone). From aazath <@t> hotmail.com Sat Mar 20 06:15:34 2010 From: aazath <@t> hotmail.com (Aazath Raj) Date: Sat Mar 20 06:15:43 2010 Subject: [Histonet] Manpower in Histopathology In-Reply-To: References: Message-ID: Dear Friends, I am an Histopathology Manager .I have been asked by my management to plan the manpower in Histopathology.is any one have reference to links are any article which i can use. With regards, Aazathraj.P Technical Officer Department of Histopathology _________________________________________________________________ The latest songs, trailers and more http://video.in.msn.com/ From Barry.R.Rittman <@t> uth.tmc.edu Sat Mar 20 07:39:39 2010 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Sat Mar 20 07:39:46 2010 Subject: [Histonet] Manpower in Histopathology In-Reply-To: References: , Message-ID: <75A0543E23D3A7458012D9E02EDBEC00098E3D7E47@UTHCMS1.uthouston.edu> Aazathraj Hi I believe there are more women than men histotechs so might I humbly suggest (and with tongue in cheek) the term "Peoplepower" instead of manpower? Barry. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Aazath Raj [aazath@hotmail.com] Sent: Saturday, March 20, 2010 6:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Manpower in Histopathology Dear Friends, I am an Histopathology Manager .I have been asked by my management to plan the manpower in Histopathology.is any one have reference to links are any article which i can use. With regards, Aazathraj.P Technical Officer Department of Histopathology _________________________________________________________________ The latest songs, trailers and more http://video.in.msn.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From k84as <@t> yahoo.com Sat Mar 20 08:30:56 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sat Mar 20 08:30:59 2010 Subject: [Histonet] cryostat help please! Message-ID: <118040.7378.qm@web112601.mail.gq1.yahoo.com> hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change!? unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? From k84as <@t> yahoo.com Sat Mar 20 11:29:20 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sat Mar 20 11:29:24 2010 Subject: [Histonet] cryostat help please! In-Reply-To: <4BA4E3C4.3030006@comcast.net> Message-ID: <400932.65463.qm@web112618.mail.gq1.yahoo.com> thanks for your replay ray yes i set the temp. to -20 degrees but it is 20 for the last week. --- On Sat, 3/20/10, Mark Ray wrote: From: Mark Ray Subject: Re: [Histonet] cryostat help please! To: "mohamed abd el razik" Date: Saturday, March 20, 2010, 5:03 PM Are you sure the temperature control is set properly?? This maybe a serious problem, but it is possible that the people who do the ordinary repairs on refrigeration equipment for the university can repair it.? You should ask them if they can help you. mohamed abd el razik wrote: > hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was > working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change!? unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? > > >? ? ???_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >??? From matt <@t> techoneweb.com Sat Mar 20 13:56:25 2010 From: matt <@t> techoneweb.com (Matthew Mincer) Date: Sat Mar 20 13:54:20 2010 Subject: [Histonet] Re: Cryostat Message-ID: <4BA51A59.4040604@techoneweb.com> Hey Mohamed, What type of cryostat do you have? Also, where are you located? I know most of the independent service companies and could probably recommend one is it turns out to be a major issue. Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 From JWeems <@t> sjha.org Sun Mar 21 07:28:36 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sun Mar 21 07:29:41 2010 Subject: [Histonet] cryostat help please! References: <118040.7378.qm@web112601.mail.gq1.yahoo.com> Message-ID: <27648A6C5BC9B145813DF5182F83AB45017D83@ITSSSXM01V1.one.ads.che.org> Sounds like it may be a fuse... in my non-expert opinion!! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of mohamed abd el razik Sent: Sat 3/20/2010 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat help please! hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change!? unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From k84as <@t> yahoo.com Sun Mar 21 11:16:25 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sun Mar 21 11:16:33 2010 Subject: [Histonet] cryostat help please! In-Reply-To: <603669.45314.qm@web83910.mail.sp1.yahoo.com> Message-ID: <450487.58504.qm@web112619.mail.gq1.yahoo.com> our cryostat is Minotome Plus Microtome Cryostat Cat. No. 2563-- For 120 VAC, 60Hz ? --- On Sat, 3/20/10, Cheryl Cornett-Early wrote: From: Cheryl Cornett-Early Subject: Re: [Histonet] cryostat help please! To: "mohamed abd el razik" Date: Saturday, March 20, 2010, 10:59 PM Hi Mohamed, ???????????? I AM a Services guy and this problem sounds like a loss of refrigerant I come across this problem on older Cryostat's after they have been turned off regularly, What? model of cryostat is it?. IT sounds like a CM1800 Riechet Jung or (Leica). Best regards Brian. My territory is the south east, GA, AL, TN, SC, NC, --- On Sat, 3/20/10, mohamed abd el razik wrote: From: mohamed abd el razik Subject: [Histonet] cryostat help please! To: histonet@lists.utsouthwestern.edu Date: Saturday, March 20, 2010, 9:30 AM hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change!? unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? Histonet@lists.utsouthwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From k84as <@t> yahoo.com Sun Mar 21 11:34:00 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sun Mar 21 11:34:06 2010 Subject: [Histonet] RE: cryostat help Message-ID: <336321.82780.qm@web112607.mail.gq1.yahoo.com> ?i forget to? introduce my self. i'm? demonestrator? of histology in faculty of? vet. med. Cairo University- Egypt.(nearly 2 years experience)?and i'm so happy to know you all histoneters. From sweething63 <@t> msn.com Sun Mar 21 11:38:43 2010 From: sweething63 <@t> msn.com (R J VAZQUEZ) Date: Sun Mar 21 11:38:49 2010 Subject: [Histonet] cryostat help please! In-Reply-To: <27648A6C5BC9B145813DF5182F83AB45017D83@ITSSSXM01V1.one.ads.che.org> References: <118040.7378.qm@web112601.mail.gq1.yahoo.com>, <27648A6C5BC9B145813DF5182F83AB45017D83@ITSSSXM01V1.one.ads.che.org> Message-ID: I agree with Joyce, it sounds like the fuse for the refrigeration. I had that problem a few times. Robyn Vazquez > Date: Sun, 21 Mar 2010 08:28:36 -0400 > From: JWeems@sjha.org > To: k84as@yahoo.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] cryostat help please! > CC: > > Sounds like it may be a fuse... in my non-expert opinion!! j > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of mohamed abd el razik > Sent: Sat 3/20/2010 9:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cryostat help please! > > hi all histoneters > i have learned alot from your posts and i need any suggestion about a cryostat that was > working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mshaeffer <@t> cox.net Sun Mar 21 16:55:36 2010 From: mshaeffer <@t> cox.net (Marc & Sandy Shaeffer) Date: Sun Mar 21 16:55:43 2010 Subject: [Histonet] Cryostat Help Please Message-ID: <3959117.11550.1269208536439.JavaMail.mshaeffer@127.0.0.1> On behalf of mohamed abd el razik QUESTION: hi all histoneters > i have learned alot from your posts and i need any suggestion about a > cryostat that was > working perfectly until the power cable is pluged off accidently. then > after about 2 days i put it again in the power and all is working with > its motor sound but no refregration and room temp. not decrease under > 20 degrees!!! i let it for aweek but no change! unfortene the > technical service may take long time till it come to see the proplem > as it is in a national university ( routine work) and i hope i could > do that. any suggestion please? MY ANSWER: With the cryostat plugged into power, I would recycle power to the unit by turning the power switch to "off", wait a couple seconds, and then return the power switch to "on". This could possibly reset the logic in the units power circuit. Just a thought... From malbenatti <@t> googlemail.com Sun Mar 21 17:12:26 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Sun Mar 21 17:12:35 2010 Subject: [Histonet] UK Trained and fully HPC Registered Histotechnologist looking to relocate in the US Message-ID: <0C0DBF56-7DE6-489E-B8CD-AD218C0F48EA@gmail.com> To all Histotechnologist State Side, I am fully qualified in the UK in all aspect of the work in histology with 8 years post UK Health Professional Council (HPC) Registration (2 years in General Pathology, 6 years in Pediatric Pathology). I was wondering if someone on the Histonet list has ever sponsored/employed a Histotechologist who trained in the UK and then went to work in the US. I am currently looking to relocate to the US and would like to inquire as to how I can convert my UK HPC registration into a US ASCP Certification, so that I may qualify to apply for histotechnologist jobs in the US. Kind regards, Malika From abright <@t> brightinstruments.com Mon Mar 22 07:49:58 2010 From: abright <@t> brightinstruments.com (Alan Bright) Date: Mon Mar 22 07:50:04 2010 Subject: [Histonet] cryostat help please! In-Reply-To: <118040.7378.qm@web112601.mail.gq1.yahoo.com> References: <118040.7378.qm@web112601.mail.gq1.yahoo.com> Message-ID: <3EFBB875DEE1994FB040A0B099F3AC8A0AD0ED@BRIGHT-SBS.Bright.local> Dear Mohamed, We manufacture cryostats but not the one you have the problem with, however I think what you can hear is the cooling fan working but not the refrigeration compressor. You will need to locate the internal fuses to see which ones need replacing. If the compressor is running then it would seem to have lost its refrigeration gas. Unfortunately I am leaving my office now and Histonet does not seem to like my Blackberry, but if you would like to talk further on this problem you can catch me on Skype dazzle0 later today or let me have your phone number and I will call you. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: 20 March 2010 13:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat help please! hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change!? unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Mon Mar 22 08:10:58 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Mar 22 08:11:05 2010 Subject: [Histonet] UK Trained and fully HPC Registered Histotechnologistlooking to relocate in the US In-Reply-To: <0C0DBF56-7DE6-489E-B8CD-AD218C0F48EA@gmail.com> References: <0C0DBF56-7DE6-489E-B8CD-AD218C0F48EA@gmail.com> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB22669E54B@chi2k3ms01.columbuschildrens.net> Malika, Best bet is to contact American Society for Clinical Pathology, http://www.ascp.org and a very good immigration lawyer. I came over here almost 20 years ago and it was a nightmare. ASCP did not recognize any British qualifications and I had then been working in pathology for 18+ years. Ronnie Houston -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malika Benatti Sent: Sunday, March 21, 2010 6:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] UK Trained and fully HPC Registered Histotechnologistlooking to relocate in the US To all Histotechnologist State Side, I am fully qualified in the UK in all aspect of the work in histology with 8 years post UK Health Professional Council (HPC) Registration (2 years in General Pathology, 6 years in Pediatric Pathology). I was wondering if someone on the Histonet list has ever sponsored/employed a Histotechologist who trained in the UK and then went to work in the US. I am currently looking to relocate to the US and would like to inquire as to how I can convert my UK HPC registration into a US ASCP Certification, so that I may qualify to apply for histotechnologist jobs in the US. Kind regards, Malika_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From andreahooper <@t> rocketmail.com Mon Mar 22 08:28:06 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Mon Mar 22 08:28:11 2010 Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer In-Reply-To: Message-ID: <102568.43045.qm@web113115.mail.gq1.yahoo.com> We used to do overnight in our DAKO Autostainer many years ago,?and we kept many containers of dH20 in the incubator to make a humid chamber. Seemed to work well - although we realized overnight was overkill and 1-2 h was sufficient.?In fact, given this we started to always put humid chambers inside for all incubations, short or long, as even after 1h we noticed some edge drying on the sections. It improved the quality and reliability of the sections. ? Andrea --- On Thu, 3/18/10, Margaryan, Naira wrote: From: Margaryan, Naira Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer To: "histonet@lists.utsouthwestern.edu" Date: Thursday, March 18, 2010, 7:38 PM Hi Histonetters! I have a stupid question but I Have to ask. Does anyone perform an Incubation of Ab that required more then 1 hour (2-3 hours) in autostainer? Does autostainer keep slides wet or slides sometimes are getting dry? I know that slides should not be dry in any step of IHC. Thank you very much! Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKnutson <@t> primecare.org Mon Mar 22 09:04:53 2010 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Mon Mar 22 09:05:00 2010 Subject: [Histonet] IHC double stains Message-ID: <1E0E2B14C709174B8AC2BE0AE7F768338FE967C6CB@EXCHANGE2K7.staprimecare.org> Fellow Histonetters, We presently use DAB brown chromagen for our IHC, and are looking at adding a red kit chromagen for staining double IHC slides. I am looking for information on what antibody scenarios other sites are using the double stain for? Kappa and lambda might be one pair of antibodies, or a p63 and prostate racemase cocktail where one is tagged red and the other brown? Would anyone be willing to share with me their scenarios where they are presently using the double stain? And how is that working for you? Thank you very much. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From sjkitten <@t> live.com Mon Mar 22 09:26:13 2010 From: sjkitten <@t> live.com (S R) Date: Mon Mar 22 09:26:17 2010 Subject: [Histonet] Prostate Bx's Message-ID: Good Morning Everyone, I was wondering for those of you working in POL labs or even hospitals, if you are pre-lableing specimen bottles before the patient comes in? Ie 12 part prostate boxes have the patient's name and the bx site pre-made days or weeks before the patient comes in. If not do you know of any documentation that states this should not be done. I have been looking for something that says the bottles should not be pre-labeled before the patient is seen, but have unfortunatley have not been able to find any. thanks in advanced sammy _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3 From abuchiane <@t> bmhvt.org Mon Mar 22 09:26:39 2010 From: abuchiane <@t> bmhvt.org (Anita Buchiane) Date: Mon Mar 22 09:26:46 2010 Subject: [Histonet] Psyche information Systems Message-ID: <602863D272B56749A70CBA315D7DC70204929EA6@bmhexch.bmhvt.org> We are currently upgrading our Psyche/WindoPath Anatomic Pathology Information System from version 5 to 7. Has anyone else gone through or presently going through this upgrade? Could you please give me an account of your experience? Good or bad. Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ From flnails <@t> texaschildrens.org Mon Mar 22 09:44:48 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon Mar 22 09:44:55 2010 Subject: [Histonet] Prostate Bx's In-Reply-To: References: Message-ID: Why would you want to prelabel the containers prior to seeing the patient? Outside of the kit being labeled LLB LLM LLA, etc., the individual containers should not be label until you actually add the sample to avoid mistakes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of S R Sent: Monday, March 22, 2010 9:26 AM To: histo net Subject: [Histonet] Prostate Bx's Good Morning Everyone, I was wondering for those of you working in POL labs or even hospitals, if you are pre-lableing specimen bottles before the patient comes in? Ie 12 part prostate boxes have the patient's name and the bx site pre-made days or weeks before the patient comes in. If not do you know of any documentation that states this should not be done. I have been looking for something that says the bottles should not be pre-labeled before the patient is seen, but have unfortunatley have not been able to find any. thanks in advanced sammy _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From DKBoyd <@t> chs.net Mon Mar 22 11:07:29 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Mon Mar 22 11:07:42 2010 Subject: [Histonet] Prostate Bx's In-Reply-To: Message-ID: National Safety Goals state that you have to label the container in front of the patient. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Nails, Felton" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/22/2010 10:45 AM To "'S R'" , "histo net" cc Subject RE: [Histonet] Prostate Bx's Why would you want to prelabel the containers prior to seeing the patient? Outside of the kit being labeled LLB LLM LLA, etc., the individual containers should not be label until you actually add the sample to avoid mistakes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of S R Sent: Monday, March 22, 2010 9:26 AM To: histo net Subject: [Histonet] Prostate Bx's Good Morning Everyone, I was wondering for those of you working in POL labs or even hospitals, if you are pre-lableing specimen bottles before the patient comes in? Ie 12 part prostate boxes have the patient's name and the bx site pre-made days or weeks before the patient comes in. If not do you know of any documentation that states this should not be done. I have been looking for something that says the bottles should not be pre-labeled before the patient is seen, but have unfortunatley have not been able to find any. thanks in advanced sammy _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Joyce.Kwan <@t> elan.com Mon Mar 22 11:44:05 2010 From: Joyce.Kwan <@t> elan.com (Kwan, Joyce) Date: Mon Mar 22 11:44:10 2010 Subject: [Histonet] Re: cryostat In-Reply-To: References: Message-ID: <64320CF42E347D4E8A1EC3D2CB26B6D51CB372@PHXEXCMB-PN03.ecorp.egn> Reply to cryostat issue: If the cryostat has a foot pedal attachment, like the Leica CM3000, sometimes the chamber will not cool if the foot pedal is not plugged in. Best, Joyce Kwan Elan Pharmaceuticals From: mohamed abd el razik Subject: [Histonet] cryostat help please! To: histonet@lists.utsouthwestern.edu Date: Saturday, March 20, 2010, 9:30 AM hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change!? unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? ******************************************************** This communication and any files transmitted with it may contain information that is confidential, privileged and exempt from disclosure under applicable law. It is intended solely for the use of the individual or entity to which it is addressed. If you are not the intended recipient, you are hereby notified that any use, dissemination or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender. Thank you for your co-operation. ******************************************************** From bob.nienhuis <@t> gmail.com Mon Mar 22 11:46:00 2010 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Mon Mar 22 11:46:04 2010 Subject: [Histonet] Spencer AO 820 microtome questions In-Reply-To: References: Message-ID: <45109da51003220946o24fab732sb52a75a44a09a88b@mail.gmail.com> How old are those 820s and 860s anyway? I know they were around in the early 70's. We still use our 860! Bob Nienhuis VA / UCLA Medical Center North Hills, CA On 3/16/10, Scott Parker wrote: > > Dear all, > > I have access to a Spencer AO 820 microtome (a "black beauty" in > Histo-parlance) that is does not have a disposable blade holder nor a > chuck > for holding paraffin cassettes. The microtome otherwise appears to be in > good condition. I would like to get suggestions for where I might be able > to > purchase a chuck and disposable blade holder for this classic model. > Alternatively, would it be a better idea for me to put this piece of > equipment on display in a glass case and instead purchase a newer > microtome. > My work involves relatively low volume, basic sectioning of paraffin blocks > for teaching and research. I don't need anything too fancy, just a > microtome > that is reliable and appropriate for student researchers to use. > > Thank you in advance for your responses. > > Scott > > > Scott L. Parker, Ph.D. > > Assistant Professor Biology > > Coastal Carolina University > > P.O. Box 261954 > > Conway, SC 29528-6054 > > > > Office Phone: (843)-349-2491 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From contact <@t> excaliburpathology.com Mon Mar 22 11:50:23 2010 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Mar 22 11:50:27 2010 Subject: [Histonet] Spencer AO 820 microtome questions In-Reply-To: <45109da51003220946o24fab732sb52a75a44a09a88b@mail.gmail.com> References: <45109da51003220946o24fab732sb52a75a44a09a88b@mail.gmail.com> Message-ID: <299795.62441.qm@web1112.biz.mail.sk1.yahoo.com> I am still using 5 820s!!! ________________________________ From: Bob Nienhuis To: Scott Parker Cc: histonet@lists.utsouthwestern.edu Sent: Mon, March 22, 2010 11:46:00 AM Subject: Re: [Histonet] Spencer AO 820 microtome questions How old are those 820s and 860s anyway? I know they were around in the early 70's. We still use our 860! Bob Nienhuis VA / UCLA Medical Center North Hills, CA On 3/16/10, Scott Parker wrote: > > Dear all, > > I have access to a Spencer AO 820 microtome (a "black beauty" in > Histo-parlance)? that is does not have a disposable blade holder nor a > chuck > for holding paraffin cassettes. The microtome otherwise appears to be in > good condition. I would like to get suggestions for where I might be able > to > purchase a chuck and disposable blade holder for this classic model. > Alternatively, would it be a better idea for me to put this piece of > equipment on display in a glass case and instead purchase a newer > microtome. > My work involves relatively low volume, basic sectioning of paraffin blocks > for teaching and research. I don't need anything too fancy, just a > microtome > that is reliable and appropriate for student researchers to use. > > Thank you in advance for your responses. > > Scott > > > Scott L. Parker, Ph.D. > > Assistant Professor Biology > > Coastal Carolina University > > P.O. Box? 261954 > > Conway, SC 29528-6054 > > > > Office Phone: (843)-349-2491 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 <@t> yahoo.com Mon Mar 22 12:17:55 2010 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Mon Mar 22 12:18:00 2010 Subject: [Histonet] Mohs Tech needed in Phoenix AZ Message-ID: <164227.94333.qm@web56804.mail.re3.yahoo.com> Dermatology group in Phoenix looking for independent experienced Mohs tech to come 1 ? 2 days per month for office Mohs surgery Ideal situation for someone wanting to pick up extra income, work with world-renowned Mohs tech and establish long-term Relationship with one of the top dermatology groups in Arizona with the opportunity for a future full-time position. This position is available immediately starting Thursday, March 25, 2010. Please fax resume to (480) 718-7342 or call (480) 585-7474 and ask for Dr. Johnson. From tao_janet <@t> yahoo.com Mon Mar 22 12:34:57 2010 From: tao_janet <@t> yahoo.com (Ms Janet Tao) Date: Mon Mar 22 12:35:01 2010 Subject: [Histonet] ub subscribe Message-ID: <981024.45505.qm@web57004.mail.re3.yahoo.com> please unsubscribe me From MLunetta <@t> luhcares.org Mon Mar 22 13:45:52 2010 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Mon Mar 22 13:46:00 2010 Subject: [Histonet] Sakura VIP 6 vs Thermo Scientific Excelsior Message-ID: <4BA76680020000A800041306@ns.luhcares.org> Hello fellow Tech's I know this is a repeat posting. I wanted to post again to get responces from some fellow tech's that might have missed the last one. We are looking at getting a new procesor and wanted to get our peers opinons on the Sakura vs Richard Allen. If you are a vendor please do not call or e-mail about processors used or otherwise. The last time I had to feild several calls and we do not want a sales pitch. Thanks, Matt HT (ASCP) Longmont United Hospital From carrolpb <@t> umdnj.edu Mon Mar 22 14:09:46 2010 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Mar 22 14:09:58 2010 Subject: [Histonet] ub subscribe In-Reply-To: <981024.45505.qm@web57004.mail.re3.yahoo.com> References: <981024.45505.qm@web57004.mail.re3.yahoo.com> Message-ID: sometimes, i feel like im stuck in some histonet unsubscribe timewarp. i hate it! i really wish that the histonet admins would address this 'unsubscribe' madness once and for all. the periodic notices on how to unsubscribe arent working and sadly, the casual subscriber has proven that they dont know how to properly use a listserv...? someone needs to rethink how this list is administrated. i mean no offense, just constructive criticism. if theres any way any of us can help out, im sure many of us with various expertises would gladly volunteer, just say the word! i am confident that i speak for all of us when i say that this repetitive, mindless unsubscribe business really detracts from the quality of my histonet experience, and theres no reason why we cant work together to fix it cone and for all :) ----- Original Message ----- From: Ms Janet Tao Date: Monday, March 22, 2010 1:35 pm Subject: [Histonet] ub subscribe To: Histonet@lists.utsouthwestern.edu > please unsubscribe me > > > ????? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rick.Garnhart <@t> memorialhealthsystem.com Mon Mar 22 14:39:40 2010 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Mon Mar 22 14:39:48 2010 Subject: [Histonet] Grossing Room Staffing In-Reply-To: Message-ID: I need help in convincing my Pathology group that it is old school to have a aid or tech in the gross room to hand them specimen and put lids on cassettes and close them. We have a PA that is employed by the pathology group that wants help in putting lids on and closing cassette. We use the Thermo-Cassette printer in the gross room and print cassettes from a bar-coded label on the container. No cassettes are preprinted and placed on the specimen container lids. Any help is greatly appreciated. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From jaflleje <@t> carisdx.com Mon Mar 22 15:22:22 2010 From: jaflleje <@t> carisdx.com (Aflleje, James) Date: Mon Mar 22 15:22:28 2010 Subject: [Histonet] RE. Research Use Only Antibodies Message-ID: Hello all, Does anyone know the exact regulations, implications, and documentation of the use of "research use only" antibodies in a CLIA RAP accredited laboratory? Does anyone else out there use RUO's in their laboratories? Input on this topic would be greatly appreciated. Thanks James D.V. Aflleje, HT(ASCP) Anatomical Pathology IHC Supervisor Caris Diagnostics a division of Caris Life Sciences 4207 E. Cotton Center Blvd., Phoenix , AZ, 85040 direct: (602) 648-8940 cell: (602) 621-0984 fax: (602) 648-8968 jaflleje@carisdx.com From mcauliff <@t> umdnj.edu Mon Mar 22 15:35:29 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Mar 22 15:33:26 2010 Subject: [Histonet] ub subscribe In-Reply-To: References: <981024.45505.qm@web57004.mail.re3.yahoo.com> Message-ID: <4BA7D491.3040209@umdnj.edu> Peter et al. All people have to do is read the instructions. How much simpler can one make it? Hit the delete key when you see any sort of unsubscribe message. Geoff Peter Carroll wrote: > sometimes, i feel like im stuck in some histonet unsubscribe timewarp. i hate it! > > i really wish that the histonet admins would address this 'unsubscribe' madness once and for all. the periodic notices on how to unsubscribe arent working and sadly, the casual subscriber has proven that they dont know how to properly use a listserv... someone needs to rethink how this list is administrated. i mean no offense, just constructive criticism. if theres any way any of us can help out, im sure many of us with various expertises would gladly volunteer, just say the word! > > i am confident that i speak for all of us when i say that this repetitive, mindless unsubscribe business really detracts from the quality of my histonet experience, and theres no reason why we cant work together to fix it cone and for all :) > > > > > > > ----- Original Message ----- > From: Ms Janet Tao > Date: Monday, March 22, 2010 1:35 pm > Subject: [Histonet] ub subscribe > To: Histonet@lists.utsouthwestern.edu > > >> please unsubscribe me >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From malbenatti <@t> googlemail.com Mon Mar 22 15:53:57 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Mon Mar 22 15:54:06 2010 Subject: [Histonet] UK Trained and fully HPC Registered Histotechnologist looking to relocate in the US In-Reply-To: <286042FD84EA48B6833A9224A5BBBF76@lurie.northwestern.edu> References: <286042FD84EA48B6833A9224A5BBBF76@lurie.northwestern.edu> Message-ID: Hi Bernice, Thanks for your reply and the URL, I hold a BSc Hons in Biomedical Sciences and a Post Graduate Certificate in Cellular Pathology over here in the UK. Since you mentioned in your reply that you have a Histotechnologist who trained in Romania, I would be interested to know if she apply for her job from outside the US or was she already in the US when she applied for her position. The problem that I face is although Histotechnologist are a "rare bread of Scientist" where automation has it's limitation and therefore very much in demand. Yet every time that I apply for a job, applying from outside the US, I find it very difficult to fill online pre-formatted application form, as they are designed for US based applicant. I have also sent my resume to few lab that have advertised job but, never got a feed back of any kind. Also Applying from abroad, their is the sponsorship/visa issue. I need a job to get working VISA a right now I feel like I am in a no win situation. Do you, or anyone on the histonet list reading this message who know of any recruiting company, laboratory manager, institutions in the US who are willing to deal with applicant from outside the US. Regards, Malika On 22 Mar 2010, at 14:51, Bernice Frederick wrote: > Malika, > ASCP has a list of accreditaion agencies here. You can send your transcripts > to them (for a fee of course) and they will tell you if you need any other > classes etc and what your scholastic status is. We have a tech from Romania > and there she had a CLS degree. The accreditation agency gave her the > equivalent of a BS in Medical Technology. She can take the HTL and plans to > in May. The ASCP has a study Guide and the NSH has a whole series of self > assessment books. It can be done. > If you are here for the NSH meeting in September it would help you a lot as > they have a class for persons taking the exam etc. Look at www.NSH.org. > www.ascp.org is the ascp site and there is info on acceeditaion there. > Bernice > > > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > ECOGPCO-RL > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malika > Benatti > Sent: Sunday, March 21, 2010 5:12 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] UK Trained and fully HPC Registered > Histotechnologistlooking to relocate in the US > > To all Histotechnologist States Side, > > I am fully qualified in the UK in all aspect of the work in histology with 8 > years post UK Health Professional Council (HPC) Registration (2 years in > General Pathology, 6 years in Pediatric Pathology). > > I was wondering if someone on the Histonet list has ever sponsored/employed > a Histotechologist who trained in the UK and then went to work in the US. I > am currently looking to relocate to the US and would like to inquire as to > how I can convert my UK HPC registration into a US ASCP Certification, so > that I may qualify to apply for histotechnologist jobs in the US. > > Kind regards, > > Malika_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Mon Mar 22 16:11:10 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 22 16:11:14 2010 Subject: [Histonet] Grossing Room Staffing In-Reply-To: Message-ID: <387856.77061.qm@web65713.mail.ac4.yahoo.com> I am sorry to tell you that having an aid in the gross room will increase the productivity of your PA by 25-30% because what you consider irrelevant tasks done by an aid will permit your PA to do what s/he is paid for and closing lids is not something that should be done by a better paid PA as compared with an aid salary.Ren? J. --- On Mon, 3/22/10, Rick.Garnhart@memorialhealthsystem.com wrote: From: Rick.Garnhart@memorialhealthsystem.com Subject: [Histonet] Grossing Room Staffing To: histonet@lists.utsouthwestern.edu Date: Monday, March 22, 2010, 3:39 PM I need help in convincing my Pathology group that it is old school to have a aid or tech in the gross room to hand them specimen and put lids on cassettes and close them. We have a PA that is employed by the pathology group that wants help in putting lids on and closing cassette. We use the Thermo-Cassette printer in the gross room and print cassettes from a bar-coded label on the container. No cassettes are preprinted and placed on the specimen container lids.? Any help is greatly appreciated. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph:? 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Mon Mar 22 16:30:41 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Mar 22 16:30:51 2010 Subject: [Histonet] GSH meeting Message-ID: <9BF995BC0E47744E9673A41486E24EE2242A6C5854@MERCERMAIL.MercerU.local> GSH has dropped the late fee for the meeting this weekend so it is not "too late" to register, especially those in the Atlanta area. Go to www.histosearch.com/gsh to get the program and registration form. Fill it out and bring it with you and register on site. We have a great group of vendors who support us who have the latest equipment and accessories to view. Come to the Evergreen Marriott Convention Resort in Stone Mountain this weekend. Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From suetp918 <@t> comcast.net Mon Mar 22 17:41:55 2010 From: suetp918 <@t> comcast.net (Sue) Date: Mon Mar 22 17:41:59 2010 Subject: [Histonet] Grossing Room Staffing In-Reply-To: <387856.77061.qm@web65713.mail.ac4.yahoo.com> Message-ID: <346819339.19841501269297715609.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> I happend to disagree, it does not take much time to close cassettes and I feel that the person grossing the specimen should be responsible for what is in the cassette. If that cassette is found empty the following morning who do you go to. You are stuck with a he said she said. We also use bar coding and print the cassettes at the gross station. I have three PA's and 3 residents grossing, that would mean I would need 6 extra lab aids to close cassettes. In this economy try to get those positions approved. Susan T. Paturzo HT (ASCP) Thomas Jefferson University Hospital Phila., PA From STACEY.LANGENBERG <@t> UCDENVER.EDU Mon Mar 22 18:04:05 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Mon Mar 22 18:06:17 2010 Subject: [Histonet] Grossing Room Staffing In-Reply-To: <346819339.19841501269297715609.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> References: <387856.77061.qm@web65713.mail.ac4.yahoo.com><346819339.19841501269297715609.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: <839133210.891275.1269299065214.JavaMail.rim@bda2340.bisx.prod.on.blackberry> I agree with you Sue. I have 2 lab aides 1 is full time and 1 is part time neither of which we can spare for hand holding and cassette closing. Our 2 gross techs are responsible for those blocks until they hit the processor. Too many hands in there causes too many problems.. The potential for us to lose a ditzel piece of skin in transition is too great. The grosser oreintates the specimen and closes the cassette end of story. Good luck Rick! Stacey Langenberg Cu Dermatopathology Sent via BlackBerry from T-Mobile -----Original Message----- From: Sue Date: Mon, 22 Mar 2010 16:41:55 To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Rick Garnhart Subject: Re: [Histonet] Grossing Room Staffing I happend to disagree, it does not take much time to close cassettes and I feel that the person grossing the specimen should be responsible for what is in the cassette. If that cassette is found empty the following morning who do you go to. You are stuck with a he said she said. We also use bar coding and print the cassettes at the gross station. I have three PA's and 3 residents grossing, that would mean I would need 6 extra lab aids to close cassettes. In this economy try to get those positions approved. Susan T. Paturzo HT (ASCP) Thomas Jefferson University Hospital Phila., PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bob.nienhuis <@t> gmail.com Mon Mar 22 19:28:00 2010 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Mon Mar 22 19:28:03 2010 Subject: [Histonet] Spencer AO 820 microtome questions In-Reply-To: <299795.62441.qm@web1112.biz.mail.sk1.yahoo.com> References: <45109da51003220946o24fab732sb52a75a44a09a88b@mail.gmail.com> <299795.62441.qm@web1112.biz.mail.sk1.yahoo.com> Message-ID: <45109da51003221728j5f5dde8ahfdc486047eb53fb7@mail.gmail.com> Found an old message. The American Optical Co. that first made the "820" Microtome in 1947, is now part of Leica. Bob On Mon, Mar 22, 2010 at 9:50 AM, Paula Pierce < contact@excaliburpathology.com> wrote: > I am still using 5 820s!!! > > ------------------------------ > *From:* Bob Nienhuis > *To:* Scott Parker > *Cc:* histonet@lists.utsouthwestern.edu > *Sent:* Mon, March 22, 2010 11:46:00 AM > *Subject:* Re: [Histonet] Spencer AO 820 microtome questions > > How old are those 820s and 860s anyway? I know they were around in the > early > 70's. > > We still use our 860! > > Bob Nienhuis > VA / UCLA Medical Center > North Hills, CA > > > On 3/16/10, Scott Parker wrote: > > > > Dear all, > > > > I have access to a Spencer AO 820 microtome (a "black beauty" in > > Histo-parlance) that is does not have a disposable blade holder nor a > > chuck > > for holding paraffin cassettes. The microtome otherwise appears to be in > > good condition. I would like to get suggestions for where I might be able > > to > > purchase a chuck and disposable blade holder for this classic model. > > Alternatively, would it be a better idea for me to put this piece of > > equipment on display in a glass case and instead purchase a newer > > microtome. > > My work involves relatively low volume, basic sectioning of paraffin > blocks > > for teaching and research. I don't need anything too fancy, just a > > microtome > > that is reliable and appropriate for student researchers to use. > > > > Thank you in advance for your responses. > > > > Scott > > > > > > Scott L. Parker, Ph.D. > > > > Assistant Professor Biology > > > > Coastal Carolina University > > > > P.O. Box 261954 > > > > Conway, SC 29528-6054 > > > > > > > > Office Phone: (843)-349-2491 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dcojita <@t> tampabay.rr.com Mon Mar 22 20:16:34 2010 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Mon Mar 22 20:16:37 2010 Subject: [Histonet] biohazardous waste Message-ID: Hello netters, Has anyone had any experience with instruments that grind up medical waste and treat it so that you can dispose of it in mainstream garbage? If so, does it work? Is it expensive? I've included the website of one such system below. Biomedical technology solutions. Any info you can offer would be greatly appreciated! www.bmtscorp.com . From ingrassi <@t> med.unibs.it Tue Mar 23 02:13:26 2010 From: ingrassi <@t> med.unibs.it (Sara Ingrassia) Date: Tue Mar 23 02:14:25 2010 Subject: [Histonet] unsubscribe Message-ID: <20100323071017.3BC7529AA5B@iperione.med.unibs.it> Please could you unsubscribe me? From louise.renton <@t> gmail.com Tue Mar 23 02:15:47 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Mar 23 02:15:54 2010 Subject: [Histonet] Re:ub subscribe In-Reply-To: <4BA7D491.3040209@umdnj.edu> References: <981024.45505.qm@web57004.mail.re3.yahoo.com> <4BA7D491.3040209@umdnj.edu> Message-ID: Nah...I'd miss the myriad spelling variations variations of the word "unsubscribe" On Mon, Mar 22, 2010 at 10:35 PM, Geoff McAuliffe wrote: > Peter et al. > > All people have to do is read the instructions. How much simpler can one > make it? Hit the delete key when you see any sort of unsubscribe message. > > Geoff > > > Peter Carroll wrote: > >> sometimes, i feel like im stuck in some histonet unsubscribe timewarp. i >> hate it! >> >> i really wish that the histonet admins would address this 'unsubscribe' >> madness once and for all. the periodic notices on how to unsubscribe arent >> working and sadly, the casual subscriber has proven that they dont know how >> to properly use a listserv... someone needs to rethink how this list is >> administrated. i mean no offense, just constructive criticism. if theres any >> way any of us can help out, im sure many of us with various expertises would >> gladly volunteer, just say the word! >> >> i am confident that i speak for all of us when i say that this repetitive, >> mindless unsubscribe business really detracts from the quality of my >> histonet experience, and theres no reason why we cant work together to fix >> it cone and for all :) >> >> >> >> >> >> >> ----- Original Message ----- >> From: Ms Janet Tao >> Date: Monday, March 22, 2010 1:35 pm >> Subject: [Histonet] ub subscribe >> To: Histonet@lists.utsouthwestern.edu >> >> >> >>> please unsubscribe me >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcauliff@umdnj.edu > ********************************************** > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From greenjumpyone <@t> hotmail.com Tue Mar 23 06:23:27 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Tue Mar 23 06:23:33 2010 Subject: [Histonet] (no subject) Message-ID: http://miniecosse.com/GNeac0Ij3r.html _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_1 From kgrobert <@t> rci.rutgers.edu Tue Mar 23 08:14:49 2010 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Tue Mar 23 08:14:53 2010 Subject: [Histonet] unsubscribe In-Reply-To: <20100323071017.3BC7529AA5B@iperione.med.unibs.it> References: <20100323071017.3BC7529AA5B@iperione.med.unibs.it> Message-ID: Sara and everybody else: PLEASE PAY ATTENTION. > Please could you unsubscribe me? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 From kgrobert <@t> rci.rutgers.edu Tue Mar 23 08:17:32 2010 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Tue Mar 23 08:17:38 2010 Subject: [Histonet] unsubscribe In-Reply-To: <20100323071017.3BC7529AA5B@iperione.med.unibs.it> References: <20100323071017.3BC7529AA5B@iperione.med.unibs.it> Message-ID: <4a2a272067b5e503d98102c2a7a49745.squirrel@webmail.rci.rutgers.edu> Sorry about that, I hit the Send key prematurely. Now: Click on this link that is at the bottom of EVERY Histonet email: http://lists.utsouthwestern.edu/mailman/listinfo/histonet Scroll down to the bottom of the page and follow the instructions to unsubscribe yourself. Really, the webmaster should change that line to "If you want to unsubscribe, click here.", with the link attached to the word "here". Thank you! Kathy Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 > Please could you unsubscribe me? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 From Janet.Bonner <@t> FLHOSP.ORG Tue Mar 23 08:59:03 2010 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Mar 23 09:00:24 2010 Subject: [Histonet] Re:ub subscribe References: <981024.45505.qm@web57004.mail.re3.yahoo.com> <4BA7D491.3040209@umdnj.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D4780015FB234@fhosxchmb006.ADVENTISTCORP.NET> Not to mention all of the references to Mythological Gods!! Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of louise renton Sent: Tue 3/23/2010 3:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:ub subscribe Nah...I'd miss the myriad spelling variations variations of the word "unsubscribe" On Mon, Mar 22, 2010 at 10:35 PM, Geoff McAuliffe wrote: > Peter et al. > > All people have to do is read the instructions. How much simpler can one > make it? Hit the delete key when you see any sort of unsubscribe message. > > Geoff > > > Peter Carroll wrote: > >> sometimes, i feel like im stuck in some histonet unsubscribe timewarp. i >> hate it! >> >> i really wish that the histonet admins would address this 'unsubscribe' >> madness once and for all. the periodic notices on how to unsubscribe arent >> working and sadly, the casual subscriber has proven that they dont know how >> to properly use a listserv... someone needs to rethink how this list is >> administrated. i mean no offense, just constructive criticism. if theres any >> way any of us can help out, im sure many of us with various expertises would >> gladly volunteer, just say the word! >> >> i am confident that i speak for all of us when i say that this repetitive, >> mindless unsubscribe business really detracts from the quality of my >> histonet experience, and theres no reason why we cant work together to fix >> it cone and for all :) >> >> >> >> >> >> >> ----- Original Message ----- >> From: Ms Janet Tao >> Date: Monday, March 22, 2010 1:35 pm >> Subject: [Histonet] ub subscribe >> To: Histonet@lists.utsouthwestern.edu >> >> >> >>> please unsubscribe me >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcauliff@umdnj.edu > ********************************************** > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From leiker <@t> buffalo.edu Tue Mar 23 09:11:02 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Mar 23 09:11:20 2010 Subject: [Histonet] unsubscribe In-Reply-To: <4a2a272067b5e503d98102c2a7a49745.squirrel@webmail.rci.rutgers.edu> References: <20100323071017.3BC7529AA5B@iperione.med.unibs.it> <4a2a272067b5e503d98102c2a7a49745.squirrel@webmail.rci.rutgers.edu> Message-ID: <3602FEB199AF5E9D352B4798@CDYwxp1931.ad.med.buffalo.edu> I really did not want to jump in on this, but Kathy did voice one thing that I'd been wanting to: Adding the "To unsubscribe, click here" link so that it's obvious. Maybe put it at the top instead of bottom of messages? I also wanted to add that probably most people wanting to unsubscribe are not even reading the messages at all, just hitting reply to a random message...so it may be a moot point trying to tell them. So, in light of that, how hard is it to just disregard and delete these single-word unsubscribe messages like so much spam? :-) :-) Regards, Merced --On Tuesday, March 23, 2010 9:17 AM -0400 kgrobert@rci.rutgers.edu wrote: > Sorry about that, I hit the Send key prematurely. Now: > > Click on this link that is at the bottom of EVERY Histonet email: > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Scroll down to the bottom of the page and follow the instructions to > unsubscribe yourself. > > Really, the webmaster should change that line to "If you want to > unsubscribe, click here.", with the link attached to the word "here". > > Thank you! > > Kathy > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (732) 445-6914 > >> Please could you unsubscribe me? >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (732) 445-6914 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From dchihc <@t> yahoo.com Tue Mar 23 09:22:32 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Tue Mar 23 09:22:36 2010 Subject: [Histonet] Full Time Position - Alabama Message-ID: <770266.51763.qm@web43503.mail.sp1.yahoo.com> ________________________________ At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday.?Must be ASCP registered HT or HTL (or eligible...must become registered within 18 months of hire date). Lab Automation includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura?XPress 50 rapid tissue processor, Leica cryostats. Duties include embedding, microtomy, ihc and special stains, and frozen sections. DCH is a 500 plus bed regional medical center. We process around 12,000 to 13,000 specimens annually.? Interested candidates please contact Sherrie Faulkner?(recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From kelleydurden <@t> pathology.ufl.edu Tue Mar 23 09:31:37 2010 From: kelleydurden <@t> pathology.ufl.edu (Durden, Kelley) Date: Tue Mar 23 09:31:56 2010 Subject: [Histonet] Sakura VIP 6 vs Excelsior Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE59319BDF@HSC-CMS01.ad.ufl.edu> I can't say anything positive or negative about the Thermo excelsior. I got some information from our sales rep and the processor seemed really great. But when it came down to it we had to stay true to Sakura and we were not disappointed. We have the VIP 6 and we love it. This processor has some really amazing features. I cannot enumerate all of them at this time b/c I'd take up lots and lots of space. The features we use the most that we continue to be very excited about are the solution manager, the bottle check, the automatic solution rotation, cassette count, bulk reservoir tanks, and to help reduce exposure to xylene we definitely use the tubes provided to drain and fill the xylene carboys and we no longer pour solutions in and out of the carboys. The carboys are nice though b/c they are a "wide mouth" size and there is a cap to put on the connector end so if you are walking to and from the processor with a carboy there are no spills from "sloshing" around. If you want me to go into greater detail about any of this please email me back. The menus are very user friendly. The touch screen is easy to use - we always wear gloves to keep finger prints at bay. The lid is ergonomically designed and easy to open and close. We have had to request a technician twice - once for a lid sensor and once for a soft ware upgrade / update - but both times they've had a tech out here the next day. I recommended this processor to another lab here in FL and as far as I know they are over the moon about theirs as well. One other really great thing was that after we purchased our 6 I was sent to CA for a training at Sakura and the information I received there was invaluable. The staff professionalism and knowledge was second to none and I left with a very thorough understanding of the machine. I was able to come back to our lab and provide training to the other staff that use the VIP 6. No I'm not a sales rep I am just a very happy customer. Kelley kelleydurden@pathology.ufl.edu From laurie.colbert <@t> huntingtonhospital.com Tue Mar 23 09:31:51 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Mar 23 09:31:59 2010 Subject: [Histonet] Thermo Slide and Cassette printers Message-ID: <57BE698966D5C54EAE8612E8941D768308453150@EXCHANGE3.huntingtonhospital.com> We have both the Printmate and 5 Slidemates. They are not problem-free and occasionally need a little TLC, but I love them both. Would never get rid of them. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, March 18, 2010 8:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Slide and Cassette printers Does anyone out there in Histo land have any experience with the new Thermo Scientific PrintMate and SlideMate system? Or do any of you have another system similar to this that you have experience with? We are looking into some of these systems to help reduce errors and make our laboratory more lean. I would appreciate any and all opinions and advice. Thank you. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernardgerard <@t> yahoo.com Tue Mar 23 09:44:07 2010 From: bernardgerard <@t> yahoo.com (Bernard Martin) Date: Tue Mar 23 09:44:10 2010 Subject: [Histonet] Sestrin 2 Message-ID: <404475.79788.qm@web37903.mail.mud.yahoo.com> Hi all! Has anyone used an antibody to Sestrin 2 for IHC, or seen a journal article that did? We are looking to stain for Sestrin 2, but want to make informed choice as to the antibody we choose. Thanks for any help you can give! Bern From tpodawiltz <@t> lrgh.org Tue Mar 23 09:43:32 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Mar 23 09:47:11 2010 Subject: [Histonet] RE: Sakura VIP 6 vs Excelsior In-Reply-To: <92E6B93E0A3D544C87DDDE33E7608AAE59319BDF@HSC-CMS01.ad.ufl.edu> References: <92E6B93E0A3D544C87DDDE33E7608AAE59319BDF@HSC-CMS01.ad.ufl.edu> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5B1FAE5@LRGHEXVS1.practice.lrgh.org> We have the Thermo Excelsior and love it. However, we purchase ours just before they took over Fisher. Since then they have gone down hill in both the service and customer relations areas. Great equipment, they are just lousy to deal with. Going forward, probably won't get any more equipment from them. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Durden, Kelley [kelleydurden@pathology.ufl.edu] Sent: Tuesday, March 23, 2010 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura VIP 6 vs Excelsior I can't say anything positive or negative about the Thermo excelsior. I got some information from our sales rep and the processor seemed really great. But when it came down to it we had to stay true to Sakura and we were not disappointed. We have the VIP 6 and we love it. This processor has some really amazing features. I cannot enumerate all of them at this time b/c I'd take up lots and lots of space. The features we use the most that we continue to be very excited about are the solution manager, the bottle check, the automatic solution rotation, cassette count, bulk reservoir tanks, and to help reduce exposure to xylene we definitely use the tubes provided to drain and fill the xylene carboys and we no longer pour solutions in and out of the carboys. The carboys are nice though b/c they are a "wide mouth" size and there is a cap to put on the connector end so if you are walking to and from the processor with a carboy there are no spills from "sloshing" around. If you want me to go into greater detail about any of this please email me back. The menus are very user friendly. The touch screen is easy to use - we always wear gloves to keep finger prints at bay. The lid is ergonomically designed and easy to open and close. We have had to request a technician twice - once for a lid sensor and once for a soft ware upgrade / update - but both times they've had a tech out here the next day. I recommended this processor to another lab here in FL and as far as I know they are over the moon about theirs as well. One other really great thing was that after we purchased our 6 I was sent to CA for a training at Sakura and the information I received there was invaluable. The staff professionalism and knowledge was second to none and I left with a very thorough understanding of the machine. I was able to come back to our lab and provide training to the other staff that use the VIP 6. No I'm not a sales rep I am just a very happy customer. Kelley kelleydurden@pathology.ufl.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From arvidsonkristen <@t> yahoo.com Tue Mar 23 10:12:18 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Mar 23 10:12:21 2010 Subject: [Histonet] Leica Paraplast Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> Has?anyone who uses paraplast (we use the basic one)?noticed a change in the quality of your tissue?? I have recently found out that they have changed manufacturing sites in the past couple of months.? I am having on and off issues with my skin specimens that have been going on for about 2 months or so.? Thought there may be a correlation.? Any thoughts?? From pruegg <@t> ihctech.net Tue Mar 23 10:59:52 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Mar 23 11:00:27 2010 Subject: [Histonet] help Message-ID: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off. I have done this on my hands before but never on my face. So far I have tried soaking a cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried putting some gold chloride on it to see if I could "tone" it down, to no avail. Any ideas? Make up only goes so far and lasts so long. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From jcox90 <@t> yahoo.com Tue Mar 23 11:03:45 2010 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Tue Mar 23 11:03:50 2010 Subject: Fw: [Histonet] help Message-ID: <462215.61755.qm@web56805.mail.re3.yahoo.com> I am so sorry but you just made my day, lol!! Wish I had a remedy for you, sorry.. Jill Cox HT (ASCP) ----- Forwarded Message ---- From: Patsy Ruegg To: histonet@lists.utsouthwestern.edu Sent: Tue, March 23, 2010 8:59:52 AM Subject: [Histonet] help After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off. I have done this on my hands before but never on my face. So far I have tried soaking a cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried putting some gold chloride on it to see if I could "tone" it down, to no avail. Any ideas? Make up only goes so far and lasts so long. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 41dmb41 <@t> gmail.com Tue Mar 23 11:05:59 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Tue Mar 23 11:06:25 2010 Subject: [Histonet] help In-Reply-To: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> References: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> Message-ID: So sorry... the only thing I know to fix it is time... about a week or two... :) Drew On Tue, Mar 23, 2010 at 11:59, Patsy Ruegg wrote: > After you stop laughing seriously I need some help here, apparently I got > my > fingers in some silver nitrate yesterday and touched my face under my nose > over my lip and now I have black spots that won't come off. I have done > this on my hands before but never on my face. So far I have tried soaking > a > cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried > putting some gold chloride on it to see if I could "tone" it down, to no > avail. Any ideas? Make up only goes so far and lasts so long. > > > > Regards, > > > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that > is > privileged & confidential within the meaning of applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWeems <@t> sjha.org Tue Mar 23 11:07:31 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Mar 23 11:07:41 2010 Subject: [Histonet] help In-Reply-To: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> References: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16405DDE83F@CHEXCMS10.one.ads.che.org> I think there is none... But thanks for the funny!! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, March 23, 2010 12:00 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] help After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off. I have done this on my hands before but never on my face. So far I have tried soaking a cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried putting some gold chloride on it to see if I could "tone" it down, to no avail. Any ideas? Make up only goes so far and lasts so long. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From talulahgosh <@t> gmail.com Tue Mar 23 11:09:35 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Mar 23 11:09:40 2010 Subject: [Histonet] help In-Reply-To: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> References: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> Message-ID: You could use a sharpie to fill in a killer moustache. I say go with handlebars and not with Hitler-style. Emily Shall we always be content with the ancient tinned salad of the subsidized novel? Or the tired ice-cream of poems which cry themselves to sleep in the refrigerators of the mind? -Lawrence Durrell, Clea On Tue, Mar 23, 2010 at 11:59 AM, Patsy Ruegg wrote: > After you stop laughing seriously I need some help here, apparently I got > my > fingers in some silver nitrate yesterday and touched my face under my nose > over my lip and now I have black spots that won't come off. I have done > this on my hands before but never on my face. So far I have tried soaking > a > cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried > putting some gold chloride on it to see if I could "tone" it down, to no > avail. Any ideas? Make up only goes so far and lasts so long. > > > > Regards, > > > > Patsy > > From sbreeden <@t> nmda.nmsu.edu Tue Mar 23 11:14:11 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 23 11:14:15 2010 Subject: [Histonet] Silver Lips and Fingers Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46FAB@nmdamailsvr.nmda.ad.nmsu.edu> I'm not a chemist and may shoot myself in the foot here, but if gold chloride tones silver, would it work on skin? Then you could call it a beauty spot? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From JWeems <@t> sjha.org Tue Mar 23 11:17:38 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Mar 23 11:17:44 2010 Subject: [Histonet] RE: Silver Lips and Fingers In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46FAB@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46FAB@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16405DDE844@CHEXCMS10.one.ads.che.org> You can reduce silver with perm solution but then it may curl the hair on your lip... And then it may peel everything off including the skin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, March 23, 2010 12:14 To: histonet Subject: [Histonet] Silver Lips and Fingers I'm not a chemist and may shoot myself in the foot here, but if gold chloride tones silver, would it work on skin? Then you could call it a beauty spot? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From contact <@t> excaliburpathology.com Tue Mar 23 11:19:26 2010 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Mar 23 11:19:31 2010 Subject: [Histonet] help In-Reply-To: References: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> Message-ID: <692843.80822.qm@web1106.biz.mail.sk1.yahoo.com> You could go get a set of Groucho glasses with the attached mustache and tell everyone you are just gearing up for April Fool's Day! or treat yourself to a?facial and get a chemical peel. ________________________________ From: Emily Sours To: Patsy Ruegg ; histonet@lists.utsouthwestern.edu Sent: Tue, March 23, 2010 11:09:35 AM Subject: Re: [Histonet] help You could use a sharpie to fill in a killer moustache. I say go with handlebars and not with Hitler-style. Emily Shall we always be content with the ancient tinned salad of the subsidized novel? Or the tired ice-cream of poems which cry themselves to sleep in the refrigerators of the mind? -Lawrence Durrell, Clea On Tue, Mar 23, 2010 at 11:59 AM, Patsy Ruegg wrote: > After you stop laughing seriously I need some help here, apparently I got > my > fingers in some silver nitrate yesterday and touched my face under my nose > over my lip and now I have black spots that won't come off.? I have done > this on my hands before but never on my face.? So far I have tried soaking > a > cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried > putting some gold chloride on it to see if I could "tone" it down, to no > avail.? Any ideas?? Make up only goes so far and lasts so long. > > > > Regards, > > > > Patsy > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Mar 23 11:21:53 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Tue Mar 23 11:22:00 2010 Subject: [Histonet] help In-Reply-To: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> References: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8CFFB6CB4@EXC-MBX3.cfs.le.ac.uk> Suggest you Google "Henna tattoos" and then make a feature of it with a few tasteful scrolls and patterns. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: 23 March 2010 16:00 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] help After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off. I have done this on my hands before but never on my face. So far I have tried soaking a cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried putting some gold chloride on it to see if I could "tone" it down, to no avail. Any ideas? Make up only goes so far and lasts so long. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Tue Mar 23 11:25:09 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Mar 23 11:25:25 2010 Subject: [Histonet] help In-Reply-To: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> References: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> Message-ID: Basically time -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, March 23, 2010 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] help After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off. I have done this on my hands before but never on my face. So far I have tried soaking a cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried putting some gold chloride on it to see if I could "tone" it down, to no avail. Any ideas? Make up only goes so far and lasts so long. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From diane.gladney <@t> us.army.mil Tue Mar 23 11:40:52 2010 From: diane.gladney <@t> us.army.mil (Gladney, Diane C Ms CIV USA MEDCOM MACH) Date: Tue Mar 23 11:41:14 2010 Subject: [Histonet] help (UNCLASSIFIED) In-Reply-To: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> References: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> Message-ID: <6673D9F600F29943A80ACD92795D34500110AF02@amedsermcbe042.amed.ds.army.mil> Classification: UNCLASSIFIED Caveats: NONE Patsy, I have removed Silver Nitrate on my hands by first treating the stain with Iodine solution then flushing the area with Sodium Thiosulfate solution (5% ?) then washing my hands thoroughly with soap and water. It removed the Silver Nitrate. This was a trick that one of my instructors used while I was in school. She got the idea from one of the silver stains that we were doing (Reticulin, I think). I don't know if how it will work on your face or if it will irritate the delicate skin but I think that I would try it anyway just to get rid of the black mark. Thanks for the laugh and my heart goes out to you. Diane G. Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC? 29207 Email:? diane.gladney@amedd.army.mil Phone:? 803-751- 2530 FAX:???? 803-751-7829 DSN:??? 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, March 23, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] help After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off. I have done this on my hands before but never on my face. So far I have tried soaking a cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried putting some gold chloride on it to see if I could "tone" it down, to no avail. Any ideas? Make up only goes so far and lasts so long. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE From Marilyn.A.Weiss <@t> kp.org Tue Mar 23 12:02:00 2010 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Tue Mar 23 12:02:12 2010 Subject: [Histonet] I will be out of office tomorrow 3/23/2010 returning 3/24/2010 Message-ID: I will be out of the office starting 03/23/2010 and will not return until 03/24/2010. .In my absence please ask for Mary Campbell . If this is urgent you can contact me on my cell phone number 858-472-4266. From pruegg <@t> ihctech.net Tue Mar 23 12:07:49 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Mar 23 12:08:12 2010 Subject: [Histonet] help In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16405DDE83F@CHEXCMS10.one.ads.che.org> References: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> <92AD9B20A6C38C4587A9FEBE3A30E16405DDE83F@CHEXCMS10.one.ads.che.org> Message-ID: Thanks for all the ideas, the qtip with straight bleach worked but now I have to treat the 3rd degree burns I have incurred thru all this. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Tuesday, March 23, 2010 10:08 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] help I think there is none... But thanks for the funny!! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, March 23, 2010 12:00 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] help After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off. I have done this on my hands before but never on my face. So far I have tried soaking a cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried putting some gold chloride on it to see if I could "tone" it down, to no avail. Any ideas? Make up only goes so far and lasts so long. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Lynne.Bell <@t> cvmc.org Tue Mar 23 12:13:26 2010 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Tue Mar 23 12:13:31 2010 Subject: [Histonet] help In-Reply-To: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> Message-ID: Pretty funny stuff!! I say to try the sodium thio - it removes unreduced silver. Or you could grab a Brillo pad and scrub away! This laugh was just what I needed today. Thank you. Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From aplewinski <@t> carisdx.com Tue Mar 23 12:41:08 2010 From: aplewinski <@t> carisdx.com (Plewinski, Amy) Date: Tue Mar 23 12:41:15 2010 Subject: [Histonet] Histotechnician Needed - Newton, MA (Boston) In-Reply-To: References: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice><92AD9B20A6C38C4587A9FEBE3A30E16405DDE83F@CHEXCMS10.one.ads.che.org> Message-ID: I am searching for a Histotechnician for our beautiful and busy lab Must have the knowledge necessary to assist pathology professionals with Immunohistochemistry, specimen processing, embedding, slide preparation, microtomy, and staining, special and other technical procedures performed in the laboratory. Job Responsibilities: 1. Performs routine and non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s), according to policies and procedures. Requirements: 1. Knowledge, Skills, and Experience a. Routine histology including specimen processing, embedding, microtomy, staining, and immunohistochemistry. Please contact me at: Amy Plewinski Aplewinski@carisdx.com 214-364-9271 From Maxim_71 <@t> mail.ru Tue Mar 23 12:43:18 2010 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Tue Mar 23 12:43:24 2010 Subject: [Histonet] help (UNCLASSIFIED) Message-ID: <435042602.20100323204318@mail.ru> Patsy: Chemically, silver nitrate deposites can be reduced onto the slides by 0.5% potassium ferricyanide. I am not sure that it may work onto living skin. Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From louise.renton <@t> gmail.com Tue Mar 23 12:57:39 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Mar 23 12:57:45 2010 Subject: [Histonet] help for silver Message-ID: I know we used Sodium thiosulphate and alcoholic iodine, but i cannot remember which ne first...I think the iodine?? John Kiernan - help please?? On Tue, Mar 23, 2010 at 5:59 PM, Patsy Ruegg wrote: > After you stop laughing seriously I need some help here, apparently I got > my > fingers in some silver nitrate yesterday and touched my face under my nose > over my lip and now I have black spots that won't come off. I have done > this on my hands before but never on my face. So far I have tried soaking > a > cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried > putting some gold chloride on it to see if I could "tone" it down, to no > avail. Any ideas? Make up only goes so far and lasts so long. > > > > Regards, > > > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that > is > privileged & confidential within the meaning of applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From ddreesen <@t> sbcglobal.net Tue Mar 23 13:29:03 2010 From: ddreesen <@t> sbcglobal.net (Debbie Dreesen) Date: Tue Mar 23 13:29:07 2010 Subject: [Histonet] RE: Leica Paraplast In-Reply-To: <201003231700.o2NH0hVP014125@flph259.prodigy.net> Message-ID: <96793.49248.qm@web81005.mail.mud.yahoo.com> Hi Kristen, We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block. From: histonet-request@lists.utsouthwestern.edu Subject: [Histonet] Leica Paraplast To: histonet Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Has?anyone who uses paraplast (we use the basic one)?noticed a change in the quality of your tissue?? I have recently found out that they have changed manufacturing sites in the past couple of months.? I am having on and off issues with my skin specimens that have been going on for about 2 months or so.? Thought there may be a correlation.? Any thoughts?? From pruegg <@t> ihctech.net Tue Mar 23 13:31:12 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Mar 23 13:31:51 2010 Subject: [Histonet] RE: sliver In-Reply-To: <002301cacaaf$0ee078c0$2ca16a40$@edu> References: <002301cacaaf$0ee078c0$2ca16a40$@edu> Message-ID: Yea the bleach worked. Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _____ From: Annette Featherstone [mailto:af46@buffalo.edu] Sent: Tuesday, March 23, 2010 11:34 AM To: pruegg@ihctech.net Subject: sliver Bleach works, be careful though Annette Featherstone From pruegg <@t> ihctech.net Tue Mar 23 13:33:43 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Mar 23 13:34:04 2010 Subject: [Histonet] help (UNCLASSIFIED) In-Reply-To: <435042602.20100323204318@mail.ru> References: <435042602.20100323204318@mail.ru> Message-ID: Are you trying to kill me? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Maxim Peshkov [mailto:Maxim_71@mail.ru] Sent: Tuesday, March 23, 2010 11:43 AM To: pruegg@ihctech.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] help (UNCLASSIFIED) Patsy: Chemically, silver nitrate deposites can be reduced onto the slides by 0.5% potassium ferricyanide. I am not sure that it may work onto living skin. Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From jkiernan <@t> uwo.ca Tue Mar 23 13:58:57 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Mar 23 13:59:03 2010 Subject: [Histonet] help for silver In-Reply-To: References: Message-ID: Some 35 years ago, a person older than I told me the most effective way to get silver stains off skin was to rub the affected part with slightly dampened mercuric chloride powder. He may have been right; I never tried it! I've tried things like iodine-thiosulphate and Farmer's reducer (ferricyanide + thiosulphate mixture) but my experience is that they do very little. The blackened epidermis flakes off, usually in a couple of days. John Kiernan Anatomy, UWO London, Canada. = = = ----- Original Message ----- From: louise renton Date: Tuesday, March 23, 2010 13:58 Subject: [Histonet] help for silver To: Patsy Ruegg , Histonet@lists.utsouthwestern.edu > I know we used Sodium thiosulphate and alcoholic iodine, but i cannot > remember which ne first...I think the iodine?? > > John Kiernan - help please?? > > On Tue, Mar 23, 2010 at 5:59 PM, Patsy Ruegg > wrote: > > > After you stop laughing seriously I need some help here, > apparently I got > > my > > fingers in some silver nitrate yesterday and touched my face > under my nose > > over my lip and now I have black spots that won't come > off. I have done > > this on my hands before but never on my face. So far I > have tried soaking > > a > > cloth in hydrogen peroxide hoping to bleach it with no luck, I > even tried > > putting some gold chloride on it to see if I could "tone" it > down, to no > > avail. Any ideas? Make up only goes so far and > lasts so long. > > > > > > > > Regards, > > > > > > > > Patsy > > > > > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech, LLC > > Fitzsimmons BioScience Park > > 12635 Montview Blvd. Suite 215 > > Aurora, CO 80010 > > P-720-859-4060 > > F-720-859-4110 > > wk email pruegg@ihctech.net > > web site www.ihctech.net > > > > > > > > > > This email is confidential and intended solely for the use of > the Person(s) > > ('the intended recipient') to whom it was addressed. Any views > or opinions > > presented are solely those of the author. It may contain > information that > > is > > privileged & confidential within the meaning of applicable > law. Accordingly > > any dissemination, distribution, copying, or other use of this > message, or > > any of its contents, by any person other than the intended > recipient may > > constitute a breach of civil or criminal law and is strictly > prohibited. If > > you are NOT the intended recipient please contact the sender > and dispose of > > this e-mail as soon as possible. > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Tue Mar 23 14:08:42 2010 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Tue Mar 23 14:08:44 2010 Subject: [Histonet] help (UNCLASSIFIED) In-Reply-To: References: <435042602.20100323204318@mail.ru> Message-ID: <1008286162.20100323220842@mail.ru> Patsy: Not, I wrote chemical relations between these reagents. Sorry, but I am not recommended it for your skin. Really, nothing necessary to do. After some days old keratin will fall off and you will not see any traces of silver nitrate. Sincerely, Maxim. You wrote at 23 March 2010, 21:33:43: > Are you trying to kill me? > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may > contain information that is > privileged & confidential within the meaning of > applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > -----Original Message----- > From: Maxim Peshkov [mailto:Maxim_71@mail.ru] > Sent: Tuesday, March 23, 2010 11:43 AM > To: pruegg@ihctech.net > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] help (UNCLASSIFIED) > Patsy: > Chemically, silver nitrate deposites can be > reduced onto the slides by 0.5% potassium ferricyanide. > I am not sure that it may work onto living skin. > Sincerely, > Maxim Peshkov, > Russia, > Taganrog. > mailto:Maxim_71@mail.ru > __________ ?????????? NOD32 4768 (20100113) __________ > ??? ????????? ????????? ???????????? ???????? NOD32. > http://www.eset.com -- ? ?????????, Maxim mailto:Maxim_71@mail.ru From hymclab.hymclab <@t> ministryhealth.org Tue Mar 23 14:10:33 2010 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Tue Mar 23 14:10:57 2010 Subject: [Histonet] RE: Leica Paraplast In-Reply-To: <96793.49248.qm@web81005.mail.mud.yahoo.com> References: <201003231700.o2NH0hVP014125@flph259.prodigy.net> <96793.49248.qm@web81005.mail.mud.yahoo.com> Message-ID: Oh, that is why we are suddenly using more blades. We thought that the blades were an inferior batch. Thanks for the info. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Dreesen Sent: Tuesday, March 23, 2010 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Leica Paraplast Hi Kristen, We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block. From: histonet-request@lists.utsouthwestern.edu Subject: [Histonet] Leica Paraplast To: histonet Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Has anyone who uses paraplast (we use the basic one) noticed a change in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From louise.renton <@t> gmail.com Tue Mar 23 14:16:57 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Mar 23 14:17:04 2010 Subject: [Histonet] help (UNCLASSIFIED) In-Reply-To: <1008286162.20100323220842@mail.ru> References: <435042602.20100323204318@mail.ru> <1008286162.20100323220842@mail.ru> Message-ID: Yep, I seem to remember reading somewhere that Silver nitrate was used as a wart remedy to "burn" skin off... still I feel for you - did the same thing once, and took a scrubbing brush to my skin to get it all off! 2010/3/23 Maxim Peshkov > Patsy: > Not, I wrote chemical relations between these reagents. > Sorry, but I am not recommended it for your skin. > Really, nothing necessary to do. > After some days old keratin will fall off and > you will not see any traces of silver nitrate. > Sincerely, > Maxim. > > You wrote at 23 March 2010, 21:33:43: > > > Are you trying to kill me? > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech, LLC > > Fitzsimmons BioScience Park > > 12635 Montview Blvd. Suite 215 > > Aurora, CO 80010 > > P-720-859-4060 > > F-720-859-4110 > > wk email pruegg@ihctech.net > > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the > Person(s) > > ('the intended recipient') to whom it was addressed. Any views or > opinions > > presented are solely those of the author. It may > > contain information that is > > privileged & confidential within the meaning of > > applicable law. Accordingly > > any dissemination, distribution, copying, or other use of this message, > or > > any of its contents, by any person other than the intended recipient may > > constitute a breach of civil or criminal law and is strictly prohibited. > If > > you are NOT the intended recipient please contact the sender and dispose > of > > this e-mail as soon as possible. > > > -----Original Message----- > > From: Maxim Peshkov [mailto:Maxim_71@mail.ru] > > Sent: Tuesday, March 23, 2010 11:43 AM > > To: pruegg@ihctech.net > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] help (UNCLASSIFIED) > > > Patsy: > > Chemically, silver nitrate deposites can be > > reduced onto the slides by 0.5% potassium ferricyanide. > > I am not sure that it may work onto living skin. > > Sincerely, > > Maxim Peshkov, > > Russia, > > Taganrog. > > mailto:Maxim_71@mail.ru > > > > > __________ ?????????? NOD32 4768 (20100113) __________ > > > ??? ????????? ????????? ???????????? ???????? NOD32. > > http://www.eset.com > > > > > -- > ? ?????????, > Maxim mailto:Maxim_71@mail.ru > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From histonet.nospam <@t> vneubert.com Tue Mar 23 14:41:39 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Tue Mar 23 14:41:59 2010 Subject: [Histonet] sliver help In-Reply-To: References: <002301cacaaf$0ee078c0$2ca16a40$@edu> Message-ID: <4BA91973.603@vneubert.com> Stained yourself? Nothing to be ashamed of! "Wo gehobelt wird, da fliegen Sp?ne" German saying :) From rjbuesa <@t> yahoo.com Tue Mar 23 14:47:31 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 23 14:47:36 2010 Subject: [Histonet] help In-Reply-To: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> Message-ID: <261409.87243.qm@web65714.mail.ac4.yahoo.com> Touch the affected areas with Lugol's solution. After that remove the Lugol with sodium thiosulfate. Ren? J. --- On Tue, 3/23/10, Patsy Ruegg wrote: From: Patsy Ruegg Subject: [Histonet] help To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 23, 2010, 11:59 AM After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off.? I have done this on my hands before but never on my face.? So far I have tried soaking a cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried putting some gold chloride on it to see if I could "tone" it down, to no avail.? Any ideas?? Make up only goes so far and lasts so long. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Tue Mar 23 15:08:00 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Mar 23 15:08:03 2010 Subject: [Histonet] Re: Grossing Room Staffing Message-ID: Rick Garnhart HT(ASCP), Memorial Health System Histology Supervisor, Colorado Springs, CO asks: >>I need help in convincing my Pathology group that it is old school to have a aid or tech in the gross room to hand them specimen and put lids on cassettes and close them. We have a PA that is employed by the pathology group that wants help in putting lids on and closing cassette. We use the Thermo-Cassette printer in the gross room and print cassettes from a bar-coded label on the container. No cassettes are preprinted and placed on the specimen container lids.<< Since I do locum tenens pathology, I frequently gross in unfamiliar surroundings. It always dismays me not to have an assistant when I gross, since my risk of mixing up specimens is high (haven't mixed one up yet). It saves a remarkable amount of time to have somebody hand me specimens and take away bottles, close cassettes, write up additional cassettes when needed, and write the embedder's record in the few labs that have this amenity (which should be required by regulatory agencies). I think the argument for a "pathologist's assistant's assistant" is even stronger. The pathologist's time is worth nothing, but the PA's time is quite valuable. Bob Richmond Samurai Pathologist Knoxville TN From Kim.Donadio <@t> bhcpns.org Tue Mar 23 15:35:13 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Mar 23 15:35:28 2010 Subject: [Histonet] help (UNCLASSIFIED) In-Reply-To: Message-ID: LOL! I am so sorry Patsy I have been reading this unfortunate but extremely funny story and I just could not help myself any more. I know this has been such a pain in the hiney for you to have to deal with and I am sure all of us here sympathize. It's still funny though < you got to just laugh it off sometimes > :-) I sincerely hope you do not have 3rd degree burns. I have no idea what can take this off other than time. I think derm abrasion might have been the only thing that might work. It's expensive though. And just to make you feel a little better. You are not the only person this has happened to, you're not alone. I think perhaps that's why many of us got such a laugh out of it. < or some of us could just want to pick> Good luck getting that off! oh and just for fun, print this whole conversation off and when this is over for you. Go back and read it for fun :o) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Patsy Ruegg" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/23/2010 01:33 PM To "'Maxim Peshkov'" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] help (UNCLASSIFIED) Are you trying to kill me? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Maxim Peshkov [mailto:Maxim_71@mail.ru] Sent: Tuesday, March 23, 2010 11:43 AM To: pruegg@ihctech.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] help (UNCLASSIFIED) Patsy: Chemically, silver nitrate deposites can be reduced onto the slides by 0.5% potassium ferricyanide. I am not sure that it may work onto living skin. Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From Allison_Scott <@t> hchd.tmc.edu Tue Mar 23 15:35:27 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Tue Mar 23 15:35:33 2010 Subject: [Histonet] Reticulin Stain Message-ID: <1872B4A455B7974391609AD8034C79FC8BD722@LBEXCH01.hchd.local> Hello to all in histoland. We are having a problem with our reticulin stain. It is not showing a real delination of the reticulin fibers. They are there but it tends to fade off. We do the brown and hopps stain. Any help would be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From billodonnell <@t> catholichealth.net Tue Mar 23 15:45:00 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Mar 23 15:45:11 2010 Subject: [Histonet] Meditech Interface In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD722@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC8BD722@LBEXCH01.hchd.local> Message-ID: Has anyone out there interfaced their cassette printer with MediTech? Sounds like it would be a real (and expensive)pain in the neck, but does it work the way you wanted it to? Thanks, William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Tuesday, March 23, 2010 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reticulin Stain Hello to all in histoland. We are having a problem with our reticulin stain. It is not showing a real delination of the reticulin fibers. They are there but it tends to fade off. We do the brown and hopps stain. Any help would be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Tue Mar 23 16:04:12 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Tue Mar 23 16:04:19 2010 Subject: [Histonet] FW: Reticulin Stain Message-ID: <1872B4A455B7974391609AD8034C79FC8BD723@LBEXCH01.hchd.local> I meant Snook Reticulin. I have the gram on the brain Allison Scott > ______________________________________________ > From: Scott, Allison D > Sent: Tuesday, March 23, 2010 3:35 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Reticulin Stain > > Hello to all in histoland. We are having a problem with our reticulin > stain. It is not showing a real delination of the reticulin fibers. > They are there but it tends to fade off. We do the brown and hopps > stain. Any help would be appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > 5656 Kelley > Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From malbenatti <@t> googlemail.com Tue Mar 23 16:16:55 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Tue Mar 23 16:17:03 2010 Subject: [Histonet] Reticulin Stain In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD722@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC8BD722@LBEXCH01.hchd.local> Message-ID: Hi Alison, Do you have a problem with all type of tissue ( Liver, Lung, Lymph Nodes, Trephine ... ) or just Trephine specimens. ? If you only have problems with Retic on BMT, if you use Formic Formaldehyde Decal fluid, see if EDTA make a difference. If you experience issue with all type of tissue. The ammoniacal silver may be at fault, make a new batch and test it with liver or reactive Lymph Node hopefully it will work. In my lab we use the Gordon & Sweet Retic. Staining protocol copy can be found in any copy of Bancroft & Stevens or the newer version Bancroft & Gamble, and Bancroft & Cook if you don'g have access to any of these book you can download the staining protocol GORDON & SWEET'S RETIC Hope this help. Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH United Kingdom Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk On Tue, Mar 23, 2010 at 8:35 PM, Scott, Allison D < Allison_Scott@hchd.tmc.edu> wrote: > Hello to all in histoland. We are having a problem with our reticulin > stain. It is not showing a real delination of the reticulin fibers. > They are there but it tends to fade off. We do the brown and hopps > stain. Any help would be appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > 5656 Kelley > Houston, Texas 77026 > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential > and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity > named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " From pathmaster <@t> yahoo.com Tue Mar 23 16:18:38 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Tue Mar 23 16:18:42 2010 Subject: [Histonet] PA baby sitter Message-ID: <578765.6618.qm@web111109.mail.gq1.yahoo.com> I had to laugh when I read this one. I'm a PA grossing 8500 surgicals per year in a busy general hospital so it's not just biopsies but lots of major organ resections with tons of orthopedics. I work with two histotechnologists. Not only do I close my own cassettes, I accession most specimens, make my own cassettes,? save and dispose of the surgical leftover tissues, serve as histology supervisor and laboratory safety officer for all sections attending all the associated meetings that those two entail, often embed at least half of the tissues and pitch in whenever I'm needed in histo- cutting and running automated specials. Having said that, if there's anyone in Europe looking for such a person and can pay 60K euro per annum , I'd love to meet you.? I'm in the opposite boat of Malika. Hey, wanna trade jobs? The techs are at my beck and call to bring me things I need,? like more acetone or formalin and/or? to attend to whatever other help I need, but no one ever sits with me to close the cassettes and feed me specimens.? I agree with the accountability issues involved in lost or mishandled tissue. What happens when the aide is off, do they expect? a histotech to come in to close the cassettes for the PA. And the productivity increase for such assistance can't be more than 5% IMHO based on my experience.? Jeff Silverman From mpence <@t> grhs.net Tue Mar 23 16:25:19 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Mar 23 16:25:24 2010 Subject: [Histonet] PA baby sitter In-Reply-To: <578765.6618.qm@web111109.mail.gq1.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DA1@is-e2k3.grhs.net> I hit it straight on Jeff! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Tuesday, March 23, 2010 4:19 PM To: Rick.Garnhart@memorialhealthsystem.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] PA baby sitter I had to laugh when I read this one. I'm a PA grossing 8500 surgicals per year in a busy general hospital so it's not just biopsies but lots of major organ resections with tons of orthopedics. I work with two histotechnologists. Not only do I close my own cassettes, I accession most specimens, make my own cassettes,? save and dispose of the surgical leftover tissues, serve as histology supervisor and laboratory safety officer for all sections attending all the associated meetings that those two entail, often embed at least half of the tissues and pitch in whenever I'm needed in histo- cutting and running automated specials. Having said that, if there's anyone in Europe looking for such a person and can pay 60K euro per annum , I'd love to meet you.? I'm in the opposite boat of Malika. Hey, wanna trade jobs? The techs are at my beck and call to bring me things I need,? like more acetone or formalin and/or? to attend to whatever other help I need, but no one ever sits with me to close the cassettes and feed me specimens.? I agree with the accountability issues involved in lost or mishandled tissue. What happens when the aide is off, do they expect? a histotech to come in to close the cassettes for the PA. And the productivity increase for such assistance can't be more than 5% IMHO based on my experience.? Jeff Silverman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Tue Mar 23 16:52:53 2010 From: azdudley <@t> hotmail.com (anita dudley) Date: Tue Mar 23 16:52:57 2010 Subject: [Histonet] number of slides Message-ID: just wondering what others were doing with colon bxs. embs, eccs. do you use one slide or cut 2 to 3 slides per block? lungs and livers too. thanks, we are thinking of going to one slide. anita dudley providence hosp. mobile alabama _________________________________________________________________ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_2 From shiningstar1713 <@t> yahoo.com Tue Mar 23 16:59:43 2010 From: shiningstar1713 <@t> yahoo.com (Janelle Powers) Date: Tue Mar 23 16:59:47 2010 Subject: [Histonet] US HT Message-ID: <469861.30359.qm@web111506.mail.gq1.yahoo.com> Hello Malika, I am not sure about sponsorship and immigration but what I can tell you is that to apply for an HT from ASCP you can take the exam which requires you to have either an associates degree with a combined?twelve credits in chemistry and biology, as well as one year experience as a histotech, or you can take a NACCLE'S accredited course to be eligible for the exam. You maybe able to convert you UK credentials?to ASCP I am not totally sure. This website should help http://www.ascp.org/FunctionalNavigation/certification/International.aspx. Hope this helps a little. I?myself wanted to know what the requirements would be??if I wanted to convert my HT ASCP into UK accreditation. Any advice you have would be great! Thanks and good luck! ? Janelle From suetp918 <@t> comcast.net Tue Mar 23 17:08:17 2010 From: suetp918 <@t> comcast.net (Sue) Date: Tue Mar 23 17:08:22 2010 Subject: [Histonet] number of slides In-Reply-To: Message-ID: <306656717.400731269382097568.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> We do three levels on all diagnostic biopsies. Liver and Kidney also get upfront special stains, as do gastric biopsies (h pylori) We did cut down on extra unstained slides since we were discarding most of them. As far as stopping levels, my pathologist thinks it goes against standard of care. As for prostate biopsies, they are so small any more, that we are thinking o cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible IHC. We are finding that when we have to go back there is minimal tumor for IHC demonstration. Susan T. Paturzo Thomas Jefferson University Hospital From malbenatti <@t> googlemail.com Tue Mar 23 17:13:19 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Tue Mar 23 17:13:23 2010 Subject: [Histonet] US HT In-Reply-To: <469861.30359.qm@web111506.mail.gq1.yahoo.com> References: <469861.30359.qm@web111506.mail.gq1.yahoo.com> Message-ID: Janelle The HPC registration rules have changed but most of the info you need are available on the IMBS website http://www.ibms.org/ and the HPC website http://www.hpc-uk.org/ If you have a recognised Degree it should only be a matter of getting your Part One the of the HPC Registration to be able to be employed has a registered BMS, then with the Part 2 you get your speciality in the discipline of your choice. Hope this help. Malika On Tue, Mar 23, 2010 at 9:59 PM, Janelle Powers wrote: > Hello Malika, > I am not sure about sponsorship and immigration but what I can tell you is > that to apply for an HT from ASCP you can take the exam which requires you > to have either an associates degree with a combined twelve credits in > chemistry and biology, as well as one year experience as a histotech, or you > can take a NACCLE'S accredited course to be eligible for the exam. You maybe > able to convert you UK credentials to ASCP I am not totally sure. This > website should help > http://www.ascp.org/FunctionalNavigation/certification/International.aspx. > Hope this helps a little. I myself wanted to know what the requirements > would be if I wanted to convert my HT ASCP into UK accreditation. Any > advice you have would be great! Thanks and good luck! > > Janelle > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " From suetp918 <@t> comcast.net Tue Mar 23 17:15:34 2010 From: suetp918 <@t> comcast.net (Sue) Date: Tue Mar 23 17:15:39 2010 Subject: [Histonet] RE: Sakura VIP 6 vs Excelsior In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323D5B1FAE5@LRGHEXVS1.practice.lrgh.org> Message-ID: <1912306703.403801269382534476.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> We also have some Exceelsior's and I am happy, I think it depends on our location. I do think that I saw a slight change in communication from Michigan, they seem a little disjointed. But I must say that the service reps that come are quite good. Susan T. Paturzo Thomas Jefferson University Hospital From rjbuesa <@t> yahoo.com Tue Mar 23 17:24:22 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 23 17:24:31 2010 Subject: [Histonet] PA baby sitter In-Reply-To: <578765.6618.qm@web111109.mail.gq1.yahoo.com> Message-ID: <827279.87357.qm@web65715.mail.ac4.yahoo.com> Tell be about it again when you get to gross 38,000 cases/year. Ren? J, --- On Tue, 3/23/10, Jeffrey Silverman wrote: From: Jeffrey Silverman Subject: [Histonet] PA baby sitter To: Rick.Garnhart@memorialhealthsystem.com Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, March 23, 2010, 5:18 PM I had to laugh when I read this one. I'm a PA grossing 8500 surgicals per year in a busy general hospital so it's not just biopsies but lots of major organ resections with tons of orthopedics. I work with two histotechnologists. Not only do I close my own cassettes, I accession most specimens, make my own cassettes,? save and dispose of the surgical leftover tissues, serve as histology supervisor and laboratory safety officer for all sections attending all the associated meetings that those two entail, often embed at least half of the tissues and pitch in whenever I'm needed in histo- cutting and running automated specials. Having said that, if there's anyone in Europe looking for such a person and can pay 60K euro per annum , I'd love to meet you.? I'm in the opposite boat of Malika. Hey, wanna trade jobs? The techs are at my beck and call to bring me things I need,? like more acetone or formalin and/or? to attend to whatever other help I need, but no one ever sits with me to close the cassettes and feed me specimens.? I agree with the accountability issues involved in lost or mishandled tissue. What happens when the aide is off, do they expect? a histotech to come in to close the cassettes for the PA. And the productivity increase for such assistance can't be more than 5% IMHO based on my experience.? Jeff Silverman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From malbenatti <@t> googlemail.com Tue Mar 23 17:33:03 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Tue Mar 23 17:33:09 2010 Subject: [Histonet] number of slides In-Reply-To: <306656717.400731269382097568.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> References: <306656717.400731269382097568.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: Routinely Gastric bx upper / rt colon / lt colon one rubbon of 4 sections per slides Liver HE @ level 1/2/3 and liver specials, AE1 IHC Renal Biopsy (Native and Transplant) 24 slides inc HE/Renal Special/ IHC and USS TransBronchial BX HE @ level 1/2/3 and lung special BMT HE, retic and IHC for NBLX case Endomyocardial BX, HE @level 1/2/3 EVG/MT and C4d IHC Vascular Malformation HE and IHC Surgical Heart (11 blocks) HE, EVG on all Blocks Cornea Button HE, PAS, AB, Congo red All Tumour BX HE, and 10 USS for IHC PM Brain HE/LFB on all blocks PM Heart HE/EVG on all blocks PM Lung HE/EVG/PERLS on all blocks Working in paediatric I have not seen a prostate of a breast for the past 6 years, but my old lab had between 4 and 6 blocks per prostate or breast tissue and were cutting HE at level 1/2/3 and picking up 4 USS between level for further IHC. Malika Malika Benatti Specialist Biomedical Scientist Great Ormond Street Hospital for Children On Tue, Mar 23, 2010 at 10:08 PM, Sue wrote: > We do three levels on all diagnostic biopsies. Liver and Kidney also get > upfront special stains, as do gastric biopsies (h pylori) > We did cut down on extra unstained slides since we were discarding most of > them. As far as stopping levels, my pathologist thinks it goes against > standard > of care. As for prostate biopsies, they are so small any more, that we are > thinking o cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for > possible IHC. We > are finding that when we have to go back there is minimal tumor for IHC > demonstration. > > Susan T. Paturzo > Thomas Jefferson University Hospital > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " From amosbrooks <@t> gmail.com Tue Mar 23 17:42:54 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Mar 23 17:43:00 2010 Subject: [Histonet] Unsubscribe Help Message-ID: <582736991003231542u3687bb35qc327b92d066c649c@mail.gmail.com> Or perhaps, More helpfully, drop them an email OFF LINE and pass along the information that is so easily found at the end of each message. I am amused by some of the messages berating the poor sots that are foolish enough to try to unsubscribe in this way (bravo, really), but the long discussions that ensue are probably more of an impedance to good discussions than the easily deleted offending unsubscribe request.. Incidentally, if anyone thinks a periodic message regarding how to unsubscribe, perhaps posting such information periodically yourself might be a good idea. We can actually do that ourselves. The moderators are busy and get nothing beyond our gratitude for their efforts. Personally I appreciate the hard work and try to ask of them only that which we cannot do ourselves such as removal of spammers & troublesome members. Just my $0.02, Amos Peter et al. All people have to do is read the instructions. How much simpler can one make it? Hit the delete key when you see any sort of unsubscribe message. Geoff Peter Carroll wrote: > sometimes, i feel like im stuck in some histonet unsubscribe timewarp. i hate it! > > i really wish that the histonet admins would address this 'unsubscribe' madness once and for all. the periodic notices on how to unsubscribe arent working and sadly, the casual subscriber has proven that they dont know how to properly use a listserv... someone needs to rethink how this list is administrated. i mean no offense, just constructive criticism. if theres any way any of us can help out, im sure many of us with various expertises would gladly volunteer, just say the word! > > i am confident that i speak for all of us when i say that this repetitive, mindless unsubscribe business really detracts from the quality of my histonet experience, and theres no reason why we cant work together to fix it cone and for all :) > From suetp918 <@t> comcast.net Tue Mar 23 17:56:47 2010 From: suetp918 <@t> comcast.net (Sue) Date: Tue Mar 23 17:56:50 2010 Subject: [Histonet] Prostate Bx's In-Reply-To: Message-ID: <1670229969.420831269385007885.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> It is never a good idea to pre-label any lab container. This is just an invitation for a specimen mix up. Susan T. Paturzo TJUH From amosbrooks <@t> gmail.com Tue Mar 23 18:07:07 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Mar 23 18:07:13 2010 Subject: [Histonet] help Message-ID: <582736991003231607qb7e6577t27fa955b897760cd@mail.gmail.com> Patsy, I'm so sorry to have laughed at your misfortune. At least you saw it coming though. You might try to get some sunlight and hope it will fade faster that way. That's a tough one the past couple of days here with the rainy CT weather. My first thought was to embrace the nice facial staining and compliment it with a bit of Nuclear Fast Red or Light Green. Best of luck, Amos Date: Tue, 23 Mar 2010 09:59:52 -0600 From: "Patsy Ruegg" Subject: [Histonet] help To: Message-ID: < 86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> Content-Type: text/plain; charset="us-ascii" After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off. I have done this on my hands before but never on my face. So far I have tried soaking a cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried putting some gold chloride on it to see if I could "tone" it down, to no avail. Any ideas? Make up only goes so far and lasts so long. Regards, Patsy From JMacDonald <@t> mtsac.edu Tue Mar 23 20:23:40 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 23 20:23:48 2010 Subject: [Histonet] Reticulin Stain In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD722@LBEXCH01.hchd.local> Message-ID: We've had problems in the past when the reducing agent is not made fresh. "Scott, Allison D" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/23/2010 01:39 PM To cc Subject [Histonet] Reticulin Stain Hello to all in histoland. We are having a problem with our reticulin stain. It is not showing a real delination of the reticulin fibers. They are there but it tends to fade off. We do the brown and hopps stain. Any help would be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lelmgren <@t> sunriselab.com Wed Mar 24 04:32:43 2010 From: lelmgren <@t> sunriselab.com (Laurie Elmgren) Date: Wed Mar 24 04:32:50 2010 Subject: [Histonet] Alcian Blue 2.5 Message-ID: <4A672C6AE0402D4A89ECE29E8A4B47E3029E9725@MailPDC.sunriselab.com> Hi Everyone, Lately, we have been having a problem with our Alcian Blue. It is fine up to the washing step. Once it hits the water, the blue fades to unacceptable. We have tried reducing the water rinse after the blue to just a quick rinse in d-H20, and it is still poor. The vendor replaced the kit, and it worked fine the first time, then two weeks later, the same problem arose. Three different techs have tried the stain three times. Any suggestions? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515x1108 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." From TMcNemar <@t> lmhealth.org Wed Mar 24 05:11:31 2010 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Mar 24 05:11:36 2010 Subject: [Histonet] number of slides In-Reply-To: <306656717.400731269382097568.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: We do 2 levels on all GIs, 3 on all needle bx, 3 on cervical, and 2 on bone marrows. We also do special stains up front on the bone marrows, H Pyloris, prostates, etc. We tend to cut a ton of extra slides that we just throw away. I don't like it but I don't think there's any way around it. Probably like most places, we get more and more smaller and smaller biopsies so it's better to keep a few extras than to try to go back later. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 23, 2010 6:08 PM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] number of slides We do three levels on all diagnostic biopsies. Liver and Kidney also get upfront special stains, as do gastric biopsies (h pylori) We did cut down on extra unstained slides since we were discarding most of them. As far as stopping levels, my pathologist thinks it goes against standard of care. As for prostate biopsies, they are so small any more, that we are thinking o cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible IHC. We are finding that when we have to go back there is minimal tumor for IHC demonstration. Susan T. Paturzo Thomas Jefferson University Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4061. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From dweishaar <@t> nmcinc.org Wed Mar 24 07:14:17 2010 From: dweishaar <@t> nmcinc.org (Diane Weishaar) Date: Wed Mar 24 07:14:23 2010 Subject: [Histonet] Meditech Interface In-Reply-To: References: <1872B4A455B7974391609AD8034C79FC8BD722@LBEXCH01.hchd.local> Message-ID: <0CBF5C420BD8AD4AAEDED774BD4A240F08E3D792@exchange.nmcinc.org> I would also like to hear from anyone using Meditech. We are currently in the process of implementing this new system with a go-live date of Oct. We have the Leica IP C and were planning on interfacing, but were just told by Meditech that we wouldn't be able to. Diane Weishaar, HT (ASCP) Histology Section Specialist Northwestern Medical Center 133 Fairfield St. St. Albans, VT 05488 Phone 802-524-1070 / Fax802-524-1098 P Please consider the environment before printing this email. The information contained in this email transmission and any attachments is intended only for the personal and confidential use of the designated named herein. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document and its attachment in error, and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify the sender and return and delete the original message immediately. Thank you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, March 23, 2010 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech Interface Has anyone out there interfaced their cassette printer with MediTech? Sounds like it would be a real (and expensive)pain in the neck, but does it work the way you wanted it to? Thanks, William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Tuesday, March 23, 2010 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reticulin Stain Hello to all in histoland. We are having a problem with our reticulin stain. It is not showing a real delination of the reticulin fibers. They are there but it tends to fade off. We do the brown and hopps stain. Any help would be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jag93 <@t> cam.ac.uk Wed Mar 24 07:27:39 2010 From: jag93 <@t> cam.ac.uk (Andrew Gillis) Date: Wed Mar 24 07:27:43 2010 Subject: [Histonet] Paraffin embedding following in situ hybridization Message-ID: Hello, Does anybody have a protocol for paraffin-embedding and sectioning embryos that have been through whole mount in situ hybridization? Thank you. Andrew -- Andrew Gillis, Ph.D. Physiology, Development & Neuroscience Anatomy Building, Downing Street Cambridge, CB2 3DY U.K. From malbenatti <@t> googlemail.com Wed Mar 24 08:52:05 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Wed Mar 24 08:52:00 2010 Subject: [Histonet] Alcian Blue 2.5 In-Reply-To: <4A672C6AE0402D4A89ECE29E8A4B47E3029E9725@MailPDC.sunriselab.com> References: <4A672C6AE0402D4A89ECE29E8A4B47E3029E9725@MailPDC.sunriselab.com> Message-ID: <15D11EDD-A425-4C1E-9A75-092A5AE47F47@gmail.com> Hey Laurie, Try with home made Alcian Blue Solution : pH 2.5 1g Alcian Blue in 100 ml of 3% Acetic Acid ( check pH adjust accordingly, filter AB solution before use) also rather than rinsing with large amount of water, blot dry section this should help alteration of staining, then rinse briefly before next step. Hope this help Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk " ... Smile it confuses people ..." On 24 Mar 2010, at 09:32, "Laurie Elmgren" wrote: > Hi Everyone, > > Lately, we have been having a problem with our Alcian Blue. It is fine > up to the washing step. Once it hits the water, the blue fades to > unacceptable. We have tried reducing the water rinse after the blue to > just a quick rinse in d-H20, and it is still poor. The vendor replaced > the kit, and it worked fine the first time, then two weeks later, the > same problem arose. Three different techs have tried the stain three > times. Any suggestions? > > > > Laurie Elmgren > > Histology Supervisor > > Sunrise Medical Labs > > 240 Motor Pkwy > > Hauppauge, NY 11788 > > (631)435-1515x1108 > > > > > > "This message contains privileged and confidential information > intended > only for the use of the addressee named above. If you are not the > intended recipient of this message you must not disseminate, copy or > take any action in reliance on it." > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Mar 24 08:58:44 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Mar 24 08:56:26 2010 Subject: [Histonet] Alcian Blue 2.5 In-Reply-To: <4A672C6AE0402D4A89ECE29E8A4B47E3029E9725@MailPDC.sunriselab.com> References: <4A672C6AE0402D4A89ECE29E8A4B47E3029E9725@MailPDC.sunriselab.com> Message-ID: <4BAA1A94.2060801@umdnj.edu> The pH of the stain may be too high, it should be 2.5-2.6 so check it with a pH meter. If the pH is fine you/your vendor may have a bad batch of dye since under normal conditions the stain is difficult to wash out. Make sure the dye is certified by the Biological Stain Commission. If there is no certification tag the dye is not certified. Geoff Laurie Elmgren wrote: > Hi Everyone, > > Lately, we have been having a problem with our Alcian Blue. It is fine > up to the washing step. Once it hits the water, the blue fades to > unacceptable. We have tried reducing the water rinse after the blue to > just a quick rinse in d-H20, and it is still poor. The vendor replaced > the kit, and it worked fine the first time, then two weeks later, the > same problem arose. Three different techs have tried the stain three > times. Any suggestions? > > > > Laurie Elmgren > > Histology Supervisor > > Sunrise Medical Labs > > 240 Motor Pkwy > > Hauppauge, NY 11788 > > (631)435-1515x1108 > > > > > > "This message contains privileged and confidential information intended > only for the use of the addressee named above. If you are not the > intended recipient of this message you must not disseminate, copy or > take any action in reliance on it." > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From tpodawiltz <@t> lrgh.org Wed Mar 24 10:19:14 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Mar 24 10:21:13 2010 Subject: [Histonet] number of slides In-Reply-To: References: <306656717.400731269382097568.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net>, Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5B1FAE8@LRGHEXVS1.practice.lrgh.org> We basically do the same at Tom's lab, the only exception is we file the unstained slides with the stained slides. Especially with the FNA biopsies. We have had to pull the unstained slide to send out for IHC's. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar [TMcNemar@lmhealth.org] Sent: Wednesday, March 24, 2010 6:11 AM To: Sue; anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] number of slides We do 2 levels on all GIs, 3 on all needle bx, 3 on cervical, and 2 on bone marrows. We also do special stains up front on the bone marrows, H Pyloris, prostates, etc. We tend to cut a ton of extra slides that we just throw away. I don't like it but I don't think there's any way around it. Probably like most places, we get more and more smaller and smaller biopsies so it's better to keep a few extras than to try to go back later. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 23, 2010 6:08 PM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] number of slides We do three levels on all diagnostic biopsies. Liver and Kidney also get upfront special stains, as do gastric biopsies (h pylori) We did cut down on extra unstained slides since we were discarding most of them. As far as stopping levels, my pathologist thinks it goes against standard of care. As for prostate biopsies, they are so small any more, that we are thinking o cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible IHC. We are finding that when we have to go back there is minimal tumor for IHC demonstration. Susan T. Paturzo Thomas Jefferson University Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4061. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From tigger13b <@t> aol.com Wed Mar 24 11:40:38 2010 From: tigger13b <@t> aol.com (tigger13b@aol.com) Date: Wed Mar 24 11:40:59 2010 Subject: [Histonet] Contamination..processor? Message-ID: <8CC9987DE39B2BA-1E28-3AC6@webmail-m050.sysops.aol.com> Hello everyone. Today we have a problem with contamination. The pathologist notes cells from tonsil specimens here and there on our GI biopsy slides. The cells are in the block. I'm trying to ascertain the source of the contamination. The grossing pathologist grossed the tonsils AFTER all GI specimens yesterday (not source of contaminant). We (the techs) embedded all GI specimens first, trimmed, cut, floated and stained ALL GI specimens BEFORE the tonsils (not source of contaminant). The only other source of the contamination I can think of is from the tissue processor. We have a Tissue Tek VIP closed processor. Has anyone ever experienced any problems like this? We had a similar issue a few weeks ago. I thought the contaminant cells may be from a bladder tumor, which had multiple sections submitted. In this instance the cells showed up days work of the bladder tumor, and in the following days work also (though the pathologists could not say for sure the cells were from the bladder case). We changed our formalin solutions in the processor and the problem did not present the next day. We also started putting all bladder tumor specimens in the microcassettes, to prevent tissue from escaping. Has anyone had any problem like this, or does anyone have any ideas on how to prevent this in the future? We had not seen this problem until these past two incidences, and this tonsil problem is particularly strange to me because we process tonsils and GI specimens in the same workload on a regular basis and have never had this issue before. Any help is appreciated! Thanks! Brandi From logunski <@t> gmail.com Wed Mar 24 11:44:39 2010 From: logunski <@t> gmail.com (Demitri Logunski) Date: Wed Mar 24 11:44:44 2010 Subject: [Histonet] Please include me at your mailing list. Thank you. Message-ID: Please include me at your mailing list. Thank you. Dima From Kim.Donadio <@t> bhcpns.org Wed Mar 24 12:00:34 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Mar 24 12:00:51 2010 Subject: [Histonet] Contamination..processor? In-Reply-To: <8CC9987DE39B2BA-1E28-3AC6@webmail-m050.sysops.aol.com> Message-ID: You are definite that the cells are in the block? If so I would change all the paraffins out as well, even the paraffin on your embedding center. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 tigger13b@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 03/24/2010 11:40 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Contamination..processor? Hello everyone. Today we have a problem with contamination. The pathologist notes cells from tonsil specimens here and there on our GI biopsy slides. The cells are in the block. I'm trying to ascertain the source of the contamination. The grossing pathologist grossed the tonsils AFTER all GI specimens yesterday (not source of contaminant). We (the techs) embedded all GI specimens first, trimmed, cut, floated and stained ALL GI specimens BEFORE the tonsils (not source of contaminant). The only other source of the contamination I can think of is from the tissue processor. We have a Tissue Tek VIP closed processor. Has anyone ever experienced any problems like this? We had a similar issue a few weeks ago. I thought the contaminant cells may be from a bladder tumor, which had multiple sections submitted. In this instance the cells showed up days work of the bladder tumor, and in the following days work also (though the pathologists could not say for sure the cells were from the bladder case). We changed our formalin solutions in the processor and the problem did not present the next day. We also started putting all bladder tumor specimens in the microcassettes, to prevent tissue from escaping. Has anyone had any problem like this, or does anyone have any ideas on how to prevent this in the future? We had not seen this problem until these past two incidences, and this tonsil problem is particularly strange to me because we process tonsils and GI specimens in the same workload on a regular basis and have never had this issue before. Any help is appreciated! Thanks! Brandi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From histonet.nospam <@t> vneubert.com Wed Mar 24 11:58:38 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Mar 24 12:05:45 2010 Subject: [Histonet] Please include me at your mailing list. Thank you. In-Reply-To: References: Message-ID: <4BAA44BE.7030009@vneubert.com> Come on, guys! Who's the first to post "Click link at bottom of mail!"? :D > Please include me at your mailing list. Thank you. > > Dima > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From leswes <@t> shaw.ca Wed Mar 24 12:11:10 2010 From: leswes <@t> shaw.ca (Lesley Weston) Date: Wed Mar 24 12:11:18 2010 Subject: [Histonet] help In-Reply-To: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> References: <86ECE24C976C4FB8950023175EF1EE11@Patsyoffice> Message-ID: Have you tried sodium thiosulphite? That will leave white spots instead of brown, which is an improvement and easier to cover. Lesley Weston. On 23-Mar-10, at 8:59 AM, Patsy Ruegg wrote: > After you stop laughing seriously I need some help here, apparently > I got my > fingers in some silver nitrate yesterday and touched my face under > my nose > over my lip and now I have black spots that won't come off. I have > done > this on my hands before but never on my face. So far I have tried > soaking a > cloth in hydrogen peroxide hoping to bleach it with no luck, I even > tried > putting some gold chloride on it to see if I could "tone" it down, > to no > avail. Any ideas? Make up only goes so far and lasts so long. > > > > Regards, > > > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the > Person(s) > ('the intended recipient') to whom it was addressed. Any views or > opinions > presented are solely those of the author. It may contain > information that is > privileged & confidential within the meaning of applicable law. > Accordingly > any dissemination, distribution, copying, or other use of this > message, or > any of its contents, by any person other than the intended > recipient may > constitute a breach of civil or criminal law and is strictly > prohibited. If > you are NOT the intended recipient please contact the sender and > dispose of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tigger13b <@t> aol.com Wed Mar 24 12:15:13 2010 From: tigger13b <@t> aol.com (tigger13b@aol.com) Date: Wed Mar 24 12:15:45 2010 Subject: [Histonet] Contamination..processor? In-Reply-To: Message-ID: <8CC998CB2C09DD1-1E28-42F4@webmail-m050.sysops.aol.com> The cells are definitely in the blocks. And we are filtering the formalin bath also. Thanks all! Brandi (prev response I received included here just for everyone's info) I would be more suspicious of the formalin "bath" that the tissues are sitting in while they await being placed on the processor. Those containers of formalin are often very cruddy by the end of the day - bits of tissue that may be on the ends of gloves, etc often end up in the container. Strain out the bits of tissue one night and see what kind of debris is floating amongst your blocks! It is very important to begin each day with a new container for formalin. Tissue can migrate too - we have seen very messy endometrial biopsies that "share" with the blocks adjacent to them in the rack. It can happen. Sheila -----Original Message----- From: Kim.Donadio@bhcpns.org To: tigger13b@aol.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wed, Mar 24, 2010 1:00 pm Subject: Re: [Histonet] Contamination..processor? You are definite that the cells are in the block? If so I would change all the paraffins out as well, even the paraffin on your embedding center. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 tigger13b@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 03/24/2010 11:40 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Contamination..processor? Hello everyone. Today we have a problem with contamination. The pathologist notes cells from tonsil specimens here and there on our GI biopsy slides. The cells are in the block. I'm trying to ascertain the source of the contamination. The grossing pathologist grossed the tonsils AFTER all GI specimens yesterday (not source of contaminant). We (the techs) embedded all GI specimens first, trimmed, cut, floated and stained ALL GI specimens BEFORE the tonsils (not source of contaminant). The only other source of the contamination I can think of is from the tissue processor. We have a Tissue Tek VIP closed processor. Has anyone ever experienced any problems like this? We had a similar issue a few weeks ago. I thought the contaminant cells may be from a bladder tumor, which had multiple sections submitted. In this instance the cells showed up days work of the bladder tumor, and in the following days work also (though the pathologists could not say for sure the cells were from the bladder case). We changed our formalin solutions in the processor and the problem did not present the next day. We also started putting all bladder tumor specimens in the microcassettes, to prevent tissue from escaping. Has anyone had any problem like this, or does anyone have any ideas on how to prevent this in the future? We had not seen this problem until these past two incidences, and this tonsil problem is particularly strange to me because we process tonsils and GI specimens in the same workload on a regular basis and have never had this issue before. Any help is appreciated! Thanks! Brandi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From Kim.Donadio <@t> bhcpns.org Wed Mar 24 12:19:17 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Mar 24 12:19:33 2010 Subject: [Histonet] Contamination..processor? In-Reply-To: <8CC998CB2C09DD1-1E28-42F4@webmail-m050.sysops.aol.com> Message-ID: You might want to check the holes that the forceps stand in on the embedding center too, if you have one like that. That seems to be a forgotten contributor at times. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 tigger13b@aol.com 03/24/2010 12:15 PM To Kim.Donadio@bhcpns.org cc histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Subject Re: [Histonet] Contamination..processor? The cells are definitely in the blocks. And we are filtering the formalin bath also. Thanks all! Brandi (prev response I received included here just for everyone's info) I would be more suspicious of the formalin "bath" that the tissues are sitting in while they await being placed on the processor. Those containers of formalin are often very cruddy by the end of the day - bits of tissue that may be on the ends of gloves, etc often end up in the container. Strain out the bits of tissue one night and see what kind of debris is floating amongst your blocks! It is very important to begin each day with a new container for formalin. Tissue can migrate too - we have seen very messy endometrial biopsies that "share" with the blocks adjacent to them in the rack. It can happen. Sheila -----Original Message----- From: Kim.Donadio@bhcpns.org To: tigger13b@aol.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wed, Mar 24, 2010 1:00 pm Subject: Re: [Histonet] Contamination..processor? You are definite that the cells are in the block? If so I would change all the paraffins out as well, even the paraffin on your embedding center. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 tigger13b@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 03/24/2010 11:40 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Contamination..processor? Hello everyone. Today we have a problem with contamination. The pathologist notes cells from tonsil specimens here and there on our GI biopsy slides. The cells are in the block. I'm trying to ascertain the source of the contamination. The grossing pathologist grossed the tonsils AFTER all GI specimens yesterday (not source of contaminant). We (the techs) embedded all GI specimens first, trimmed, cut, floated and stained ALL GI specimens BEFORE the tonsils (not source of contaminant). The only other source of the contamination I can think of is from the tissue processor. We have a Tissue Tek VIP closed processor. Has anyone ever experienced any problems like this? We had a similar issue a few weeks ago. I thought the contaminant cells may be from a bladder tumor, which had multiple sections submitted. In this instance the cells showed up days work of the bladder tumor, and in the following days work also (though the pathologists could not say for sure the cells were from the bladder case). We changed our formalin solutions in the processor and the problem did not present the next day. We also started putting all bladder tumor specimens in the microcassettes, to prevent tissue from escaping. Has anyone had any problem like this, or does anyone have any ideas on how to prevent this in the future? We had not seen this problem until these past two incidences, and this tonsil problem is particularly strange to me because we process tonsils and GI specimens in the same workload on a regular basis and have never had this issue before. Any help is appreciated! Thanks! Brandi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From tjasper <@t> copc.net Wed Mar 24 12:31:23 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Wed Mar 24 12:31:29 2010 Subject: [Histonet] Contamination..processor? References: <8CC9987DE39B2BA-1E28-3AC6@webmail-m050.sysops.aol.com> Message-ID: <90354A475B420441B2A0396E5008D49692BF2B@copc-sbs.COPC.local> Hi Brandi, Don't know if I can solve your problem...but here's a few questions. 1)You've determined that the floater (tonsil cells in this case) are in the block. Did you determine this because you consistently see the same floater in the same spot, level after level, slide after slide? 2)Do you use a forceps warmer at your embedding station? If so, have the wells of that warmer been cleaned out lately? It can be a source of floaters. 3)Do you have 2 processors? If so, are you running separate programs (on separate machines of course) for large and small specimens? If you can do this and you are not, you might want to consider it. 4)While it's possible you could be picking something up from your processor, I would not be initially suspect of it. Are you running clean runs after processing runs? If you are this should basically take care of any residual tissue floaters that may have gotten out of a (tonsil) block, or any other block for that matter. You embedded the GI biopsies first, so I would not suspect the embedding center work surfaces to be a source of your tonsil floater. Some machines have little grooves to allow waste paraffin to drain off, sometimes things can be trapped there. Also, you say that the pathologist grossed the tonsils after the GI's. I believe you and him/her, but was anything else grossed before the GI's? Some type of lymphatic tissue? I tend to look to the grossing bench 1st for the source of floaters because once it's done there, it shows up everywhere else. Also, when techs cut and embed, they have no choice but to cut and embed whatever they're given. There is no way to determine if what you might be looking at is a floater. And more often than not, when cutting and embedding you've inherited a floater as opposed to introducing one. Good luck, hope this helps. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tigger13b@aol.com Sent: Wednesday, March 24, 2010 9:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Contamination..processor? Hello everyone. Today we have a problem with contamination. The pathologist notes cells from tonsil specimens here and there on our GI biopsy slides. The cells are in the block. I'm trying to ascertain the source of the contamination. The grossing pathologist grossed the tonsils AFTER all GI specimens yesterday (not source of contaminant). We (the techs) embedded all GI specimens first, trimmed, cut, floated and stained ALL GI specimens BEFORE the tonsils (not source of contaminant). The only other source of the contamination I can think of is from the tissue processor. We have a Tissue Tek VIP closed processor. Has anyone ever experienced any problems like this? We had a similar issue a few weeks ago. I thought the contaminant cells may be from a bladder tumor, which had multiple sections submitted. In this instance the cells showed up days work of the bladder tumor, and in the following days work also (though the pathologists could not say for sure the cells were from the bladder case). We changed our formalin solutions in the processor and the problem did not present the next day. We also started putting all bladder tumor specimens in the microcassettes, to prevent tissue from escaping. Has anyone had any problem like this, or does anyone have any ideas on how to prevent this in the future? We had not seen this problem until these past two incidences, and this tonsil problem is particularly strange to me because we process tonsils and GI specimens in the same workload on a regular basis and have never had this issue before. Any help is appreciated! Thanks! Brandi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Mar 24 12:43:21 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Mar 24 12:43:26 2010 Subject: [Histonet] Re: number of slides Message-ID: Anita Dudley, Providence Hospital, Mobile Alabama asks: >>Just wondering what others were doing with colon [biopsies, endometrial biopsies], ECC's. Do you use one slide or cut 2 to 3 slides per block? Lungs and livers too. We are thinking of going to one slide.<< Do your pathologists have any input into this decision? (Often we don't.) I've worked on a large number of pathology services, and I take what I can get. Currently I'm getting one short ribbon on one slide. We've probably lost an account lately because of this curtailed examination. All of the cases you and others have mentioned should get at least two slides, preferably with two ribbons on a slide, plus whatever routine special stains the specimen requires. It's better for pathologists to have some input, given that we're the actual users of the slides, but we're pretty far down the management food chain. Bob Richmond Samurai Pathologist Knoxville TN From tigger13b <@t> aol.com Wed Mar 24 12:46:13 2010 From: tigger13b <@t> aol.com (tigger13b@aol.com) Date: Wed Mar 24 12:46:47 2010 Subject: [Histonet] Contamination..processor? In-Reply-To: <90354A475B420441B2A0396E5008D49692BF2B@copc-sbs.COPC.local> References: <8CC9987DE39B2BA-1E28-3AC6@webmail-m050.sysops.aol.com> <90354A475B420441B2A0396E5008D49692BF2B@copc-sbs.COPC.local> Message-ID: <8CC999107AD7E2D-1E28-4A36@webmail-m050.sysops.aol.com> Response to Tjapser: 1 - The cells are in the same location on all levels. 2 - We have a forceps warmer, but we burn the tip in the bunsen flame in between each case, and we hold the forceps in our hand when imbedding in succession. We do pause to trim and then go back to embedding (we are a small lab) so it is a possibility. We will be sure to clean inside the forceps warmer. 3 - We only have one processor. 4 - We do running the clean cycle every day. And all solutions were changed on Monday. We had a frozen section earlier in the day - merkel cell carcinoma skin mass - three different specimens from the same patient. However, the pathologists take the GI biopsies straight from the little formalin containers and put them directly into the microcassettes, so they don't touch the grossing table. Thanks so much for your comments/suggestions. Brandi -----Original Message----- From: Thomas Jasper To: tigger13b@aol.com Cc: histonet@lists.utsouthwestern.edu Sent: Wed, Mar 24, 2010 1:31 pm Subject: RE: [Histonet] Contamination..processor? Hi Brandi, Don't know if I can solve your problem...but here's a few questions. 1)You've determined that the floater (tonsil cells in this case) are in he block. Did you determine this because you consistently see the same loater in the same spot, level after level, slide after slide? 2)Do you use a forceps warmer at your embedding station? If so, have he wells of that warmer been cleaned out lately? It can be a source of loaters. 3)Do you have 2 processors? If so, are you running separate programs on separate machines of course) for large and small specimens? If you an do this and you are not, you might want to consider it. 4)While it's possible you could be picking something up from your rocessor, I would not be initially suspect of it. Are you running lean runs after processing runs? If you are this should basically take are of any residual tissue floaters that may have gotten out of a tonsil) block, or any other block for that matter. You embedded the GI biopsies first, so I would not suspect the embedding enter work surfaces to be a source of your tonsil floater. Some achines have little grooves to allow waste paraffin to drain off, ometimes things can be trapped there. Also, you say that the athologist grossed the tonsils after the GI's. I believe you and im/her, but was anything else grossed before the GI's? Some type of ymphatic tissue? I tend to look to the grossing bench 1st for the ource of floaters because once it's done there, it shows up everywhere lse. Also, when techs cut and embed, they have no choice but to cut nd embed whatever they're given. There is no way to determine if what ou might be looking at is a floater. And more often than not, when utting and embedding you've inherited a floater as opposed to ntroducing one. ood luck, hope this helps. Tom Jasper Thomas Jasper HT (ASCP) BAS istology Supervisor entral Oregon Regional Pathology Services end, Oregon 97701 41/693-2677 jasper@copc.net -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of igger13b@aol.com ent: Wednesday, March 24, 2010 9:41 AM o: histonet@lists.utsouthwestern.edu ubject: [Histonet] Contamination..processor? Hello everyone. Today we have a problem with contamination. The pathologist notes ells from tonsil specimens here and there on our GI biopsy slides. The ells are in the block. I'm trying to ascertain the source of the ontamination. The grossing pathologist grossed the tonsils AFTER all GI specimens esterday (not source of contaminant). We (the techs) embedded all GI pecimens first, trimmed, cut, floated and stained ALL GI specimens EFORE the tonsils (not source of contaminant). The only other source f the contamination I can think of is from the tissue processor. We ave a Tissue Tek VIP closed processor. Has anyone ever experienced any roblems like this? We had a similar issue a few weeks ago. I thought he contaminant cells may be from a bladder tumor, which had multiple ections submitted. In this instance the cells showed up days work of he bladder tumor, and in the following days work also (though the athologists could not say for sure the cells were from the bladder ase). We changed our formalin solutions in the processor and the roblem did not present the next day. We also started putting all ladder tumor specimens in the microcassettes, to prevent tissue from scaping. Has anyone had any problem like this, or does anyone have any deas on how to prevent this in the future? We had not seen this roblem until these past two incidences, and this tonsil problem is articularly strange to me because we process tonsils and GI specimens n the same workload on a regular basis and have never had this issue efore. Any help is appreciated! Thanks! randi ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet -----Original Message----- From: Thomas Jasper To: tigger13b@aol.com Cc: histonet@lists.utsouthwestern.edu Sent: Wed, Mar 24, 2010 1:31 pm Subject: RE: [Histonet] Contamination..processor? Hi Brandi, Don't know if I can solve your problem...but here's a few questions. 1)You've determined that the floater (tonsil cells in this case) are in he block. Did you determine this because you consistently see the same loater in the same spot, level after level, slide after slide? 2)Do you use a forceps warmer at your embedding station? If so, have he wells of that warmer been cleaned out lately? It can be a source of loaters. 3)Do you have 2 processors? If so, are you running separate programs on separate machines of course) for large and small specimens? If you an do this and you are not, you might want to consider it. 4)While it's possible you could be picking something up from your rocessor, I would not be initially suspect of it. Are you running lean runs after processing runs? If you are this should basically take are of any residual tissue floaters that may have gotten out of a tonsil) block, or any other block for that matter. You embedded the GI biopsies first, so I would not suspect the embedding enter work surfaces to be a source of your tonsil floater. Some achines have little grooves to allow waste paraffin to drain off, ometimes things can be trapped there. Also, you say that the athologist grossed the tonsils after the GI's. I believe you and im/her, but was anything else grossed before the GI's? Some type of ymphatic tissue? I tend to look to the grossing bench 1st for the ource of floaters because once it's done there, it shows up everywhere lse. Also, when techs cut and embed, they have no choice but to cut nd embed whatever they're given. There is no way to determine if what ou might be looking at is a floater. And more often than not, when utting and embedding you've inherited a floater as opposed to ntroducing one. ood luck, hope this helps. Tom Jasper Thomas Jasper HT (ASCP) BAS istology Supervisor entral Oregon Regional Pathology Services end, Oregon 97701 41/693-2677 jasper@copc.net -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of igger13b@aol.com ent: Wednesday, March 24, 2010 9:41 AM o: histonet@lists.utsouthwestern.edu ubject: [Histonet] Contamination..processor? Hello everyone. Today we have a problem with contamination. The pathologist notes ells from tonsil specimens here and there on our GI biopsy slides. The ells are in the block. I'm trying to ascertain the source of the ontamination. The grossing pathologist grossed the tonsils AFTER all GI specimens esterday (not source of contaminant). We (the techs) embedded all GI pecimens first, trimmed, cut, floated and stained ALL GI specimens EFORE the tonsils (not source of contaminant). The only other source f the contamination I can think of is from the tissue processor. We ave a Tissue Tek VIP closed processor. Has anyone ever experienced any roblems like this? We had a similar issue a few weeks ago. I thought he contaminant cells may be from a bladder tumor, which had multiple ections submitted. In this instance the cells showed up days work of he bladder tumor, and in the following days work also (though the athologists could not say for sure the cells were from the bladder ase). We changed our formalin solutions in the processor and the roblem did not present the next day. We also started putting all ladder tumor specimens in the microcassettes, to prevent tissue from scaping. Has anyone had any problem like this, or does anyone have any deas on how to prevent this in the future? We had not seen this roblem until these past two incidences, and this tonsil problem is articularly strange to me because we process tonsils and GI specimens n the same workload on a regular basis and have never had this issue efore. Any help is appreciated! Thanks! randi ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Mar 24 12:55:21 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Mar 24 12:55:28 2010 Subject: [Histonet] Alcian Blue 2.5 In-Reply-To: <4A672C6AE0402D4A89ECE29E8A4B47E3029E9725@MailPDC.sunriselab.com> References: <4A672C6AE0402D4A89ECE29E8A4B47E3029E9725@MailPDC.sunriselab.com> Message-ID: If the colour washes out of the stained sections, the dye is not alcian blue, whatever the label on the bottle might say. Ask for a refund. One of the criteria for certifying alcian blue for use as a biological stain is that the soluble dye becomes completely insoluble and cannot be extracted. The tests for certification are all published - Biotech. Histochem. 77:237-275, 2002 - (pp. 241-242 for alcian blue). For an additional insolubilization test and revised spectrophotometric criteria for alcian blue, see also Biotech. Histochem. 83: 201-203, 2008. If you buy a pre-made solution, check with the vendor that it contains alcian blue from a batch of dye powder that has been certified by the Biological Stain Commission (BSC). Each batch of a certified dye has a label with a number consisting of some letters (indicating the name of the stain and the company that submitted samples), a hyphen, and then a number. For example, DcAn-14 was a batch of alcian blue certified in 2009 for Dudley Chemical Co. Every bottle of certified alcian blue powder has a label with a number of that kind. Anyone making up a solution of non-certified alcian blue should not be surprised if it doesn't work properly as a stain. Unsatisfactory dyes called "alcian blue" have been around for as long as this dye has been in use as a microscopical stain (60 years). The criteria for identifying a "good" batch were set out by Scott & Mowry in 1970: "Alcian blue - a consumer's guide". J. Histochem. Cytochem. 18:842. These criteria are essentially the same as those currently used for certification by the BSC. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Laurie Elmgren Date: Wednesday, March 24, 2010 5:34 Subject: [Histonet] Alcian Blue 2.5 To: histonet@lists.utsouthwestern.edu > Hi Everyone, > > Lately, we have been having a problem with our Alcian Blue. It > is fine > up to the washing step. Once it hits the water, the blue fades to > unacceptable. We have tried reducing the water rinse after the > blue to > just a quick rinse in d-H20, and it is still poor. The vendor replaced > the kit, and it worked fine the first time, then two weeks > later, the > same problem arose. Three different techs have tried the stain three > times. Any suggestions? > > > > Laurie Elmgren > > Histology Supervisor > > Sunrise Medical Labs > > 240 Motor Pkwy > > Hauppauge, NY 11788 > > (631)435-1515x1108 > > > > > > "This message contains privileged and confidential information > intendedonly for the use of the addressee named above. If you > are not the > intended recipient of this message you must not disseminate, > copy or > take any action in reliance on it." > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kimberly.Marshall <@t> ahss.org Wed Mar 24 13:02:49 2010 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Wed Mar 24 13:04:19 2010 Subject: [Histonet] Bx sections. Message-ID: Hello all. We cut 5 levels on each GI block and mount them on one slide. We use the really small disposable molds. There are the big polyps or multiple bx in one container In that case we cut three levels . On our prostate bx we are cutting 3 levels picking up 4 slides of each level. So we end up with 4 identical slides. Well at least close, those are the slides we keep back for the possible Immunos. We attempt to keep to one slide when possible. ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From ttruscot <@t> vetmed.wsu.edu Wed Mar 24 13:37:34 2010 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Mar 24 13:37:43 2010 Subject: [Histonet] Contamination..processor? In-Reply-To: References: <8CC9987DE39B2BA-1E28-3AC6@webmail-m050.sysops.aol.com> Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB4C22D1F1F7A@CVMMBX.vetmed.wsu.edu> If not already mentioned, wiping your heated forcep off with a kimwipe or paper towel before and after embedding each tissue helps prevent this problem at embedding. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, March 24, 2010 10:01 AM To: tigger13b@aol.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Contamination..processor? You are definite that the cells are in the block? If so I would change all the paraffins out as well, even the paraffin on your embedding center. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 tigger13b@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 03/24/2010 11:40 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Contamination..processor? Hello everyone. Today we have a problem with contamination. The pathologist notes cells from tonsil specimens here and there on our GI biopsy slides. The cells are in the block. I'm trying to ascertain the source of the contamination. The grossing pathologist grossed the tonsils AFTER all GI specimens yesterday (not source of contaminant). We (the techs) embedded all GI specimens first, trimmed, cut, floated and stained ALL GI specimens BEFORE the tonsils (not source of contaminant). The only other source of the contamination I can think of is from the tissue processor. We have a Tissue Tek VIP closed processor. Has anyone ever experienced any problems like this? We had a similar issue a few weeks ago. I thought the contaminant cells may be from a bladder tumor, which had multiple sections submitted. In this instance the cells showed up days work of the bladder tumor, and in the following days work also (though the pathologists could not say for sure the cells were from the bladder case). We changed our formalin solutions in the processor and the problem did not present the next day. We also started putting all bladder tumor specimens in the microcassettes, to prevent tissue from escaping. Has anyone had any problem like this, or does anyone have any ideas on how to prevent this in the future? We had not seen this problem until these past two incidences, and this tonsil problem is particularly strange to me because we process tonsils and GI specimens in the same workload on a regular basis and have never had this issue before. Any help is appreciated! Thanks! Brandi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Mar 24 13:37:25 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Mar 24 13:37:46 2010 Subject: [Histonet] benchmark ultra and continuous workflow Message-ID: Hi Benchmark Ultra users! I would like to hear of your experiences with the continuous workflow of this instrument. Does it really make life easier or even more complicated? I think of handling one slide every few minutes after the staining is completed and of the problems, that occur when the stainer is started with "unimportant" cases and the later coming "important" cases have not enough place to complete them in time. Gudrun Lang From malbenatti <@t> googlemail.com Wed Mar 24 14:27:49 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Wed Mar 24 14:27:58 2010 Subject: [Histonet] Contamination..processor? Message-ID: <644E0267-6D0E-45CE-BDFC-973F6831A1F6@gmail.com> Hey Brandi, In my experience carry over generally occurred at time of cut-up or embedding when tiny bit of tissue get stuck between the groove of the forceps, despite been wiped clean between each specimens, but if you are 100 % sure that carry over did not occurred at time of cut-up, or embedding but during processing but during processing and the tonsil tissue is definitely in the block I suggest that you use bio-wrap/tissue wrap. Cheers, Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH United Kingdom Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk Hello everyone. Today we have a problem with contamination. The pathologist notes cells from tonsil specimens here and there on our GI biopsy slides. The cells are in the block. I'm trying to ascertain the source of the contamination. The grossing pathologist grossed the tonsils AFTER all GI specimens yesterday (not source of contaminant). We (the techs) embedded all GI specimens first, trimmed, cut, floated and stained ALL GI specimens BEFORE the tonsils (not source of contaminant). The only other source of the contamination I can think of is from the tissue processor. We have a Tissue Tek VIP closed processor. Has anyone ever experienced any problems like this? We had a similar issue a few weeks ago. I thought the contaminant cells may be from a bladder tumor, which had multiple sections submitted. In this instance the cells showed up days work of the bladder tumor, and in the following days work also (though the pathologists could not say for sure the cells were from the bladder case). We changed our formalin solutions in the processor and the problem did not present the next day. We also started putting all bladder tumor specimens in the microcassettes, to prevent tissue from escaping. Has anyone had any problem like this, or does anyone have any ideas on how to prevent this in the future? We had not seen this problem until these past two incidences, and this tonsil problem is particularly strange to me because we process tonsils and GI specimens in the same workload on a regular basis and have never had this issue before. Any help is appreciated! Thanks! Brandi From hymclab.hymclab <@t> ministryhealth.org Wed Mar 24 15:19:42 2010 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Wed Mar 24 15:19:56 2010 Subject: [Histonet] number of slides In-Reply-To: <306656717.400731269382097568.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> References: <306656717.400731269382097568.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: We follow the same practice as Susan. Our Pathologists would rather have more than less!!! Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 23, 2010 5:08 PM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] number of slides We do three levels on all diagnostic biopsies. Liver and Kidney also get upfront special stains, as do gastric biopsies (h pylori) We did cut down on extra unstained slides since we were discarding most of them. As far as stopping levels, my pathologist thinks it goes against standard of care. As for prostate biopsies, they are so small any more, that we are thinking o cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible IHC. We are finding that when we have to go back there is minimal tumor for IHC demonstration. Susan T. Paturzo Thomas Jefferson University Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From hfedor <@t> jhmi.edu Wed Mar 24 15:24:18 2010 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Wed Mar 24 15:24:24 2010 Subject: [Histonet] Cover glass Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D316BDEA39C2@JHEMTEXVS3.win.ad.jhu.edu> Hello, Our lab does IHC staining in house and have had zero problems with our Fisher brand cover slips until recently. We've tried both Fisher and Corning cover slips and what appears to be dust or imperfections in the glass are found on both brands. This is creating false positives for us, because when the positive staining is low it is difficult to impossible to tell the imperfections from the staining itself. Any advice will be welcome. -- Helen From talulahgosh <@t> gmail.com Wed Mar 24 15:26:46 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Mar 24 15:26:51 2010 Subject: [Histonet] Cover glass In-Reply-To: <3201CF51728F6048A24FA3AFFFEEF1D316BDEA39C2@JHEMTEXVS3.win.ad.jhu.edu> References: <3201CF51728F6048A24FA3AFFFEEF1D316BDEA39C2@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: I've noticed Corning cover slips have been really dirty too, but it hasn't been a problem for us. It just looks bad. Not that that solved your problem, just thought I'd throw it out there. Emily S. Shall we always be content with the ancient tinned salad of the subsidized novel? Or the tired ice-cream of poems which cry themselves to sleep in the refrigerators of the mind? -Lawrence Durrell, Clea On Wed, Mar 24, 2010 at 4:24 PM, Helen Fedor wrote: > Hello, Our lab does IHC staining in house and have had zero problems with our Fisher brand cover slips until recently. We've tried both Fisher and Corning cover slips and what appears to be dust or imperfections in the glass are found on both brands. This is creating false positives for us, because when the positive staining is low it is difficult to impossible to tell the imperfections from the staining itself. > > Any advice will be welcome. > > -- > Helen > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From leiker <@t> buffalo.edu Wed Mar 24 15:33:35 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Mar 24 15:33:42 2010 Subject: [Histonet] Cover glass In-Reply-To: <3201CF51728F6048A24FA3AFFFEEF1D316BDEA39C2@JHEMTEXVS3.win.ad.jhu.edu> References: <3201CF51728F6048A24FA3AFFFEEF1D316BDEA39C2@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: <2C0FB9C38453F4C9E0B2378A@CDYwxp1931.ad.med.buffalo.edu> I use VWR VistaVision coverslips (for fluorescent imaging) and had noticed some dust/debris on them that would autofluoresce and give false positives with some of my stains. The problem disappeared when I got into the habit wiping all my coverslips with EtOH before mounting...this may be cumbersome if you're doing a lot of slides, however. But it has worked really well. Regards, Merced --On Wednesday, March 24, 2010 4:24 PM -0400 Helen Fedor wrote: > Hello, Our lab does IHC staining in house and have had zero problems with > our Fisher brand cover slips until recently. We've tried both Fisher and > Corning cover slips and what appears to be dust or imperfections in the > glass are found on both brands. This is creating false positives for us, > because when the positive staining is low it is difficult to impossible > to tell the imperfections from the staining itself. > > Any advice will be welcome. > > -- > Helen > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From FUNKM <@t> mercyhealth.com Wed Mar 24 15:35:15 2010 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed Mar 24 15:35:27 2010 Subject: [Histonet] Cover glass In-Reply-To: References: <3201CF51728F6048A24FA3AFFFEEF1D316BDEA39C2@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: <4BAA3133.9B87.00AC.0@mercyhealth.com> Emily, yes we have also noticed real dirty glass also. I will be calling and checking on what has changed. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> Emily Sours 03/24/2010 3:26 PM >>> I've noticed Corning cover slips have been really dirty too, but it hasn't been a problem for us. It just looks bad. Not that that solved your problem, just thought I'd throw it out there. Emily S. Shall we always be content with the ancient tinned salad of the subsidized novel? Or the tired ice-cream of poems which cry themselves to sleep in the refrigerators of the mind? -Lawrence Durrell, Clea On Wed, Mar 24, 2010 at 4:24 PM, Helen Fedor wrote: > Hello, Our lab does IHC staining in house and have had zero problems with our Fisher brand cover slips until recently. We've tried both Fisher and Corning cover slips and what appears to be dust or imperfections in the glass are found on both brands. This is creating false positives for us, because when the positive staining is low it is difficult to impossible to tell the imperfections from the staining itself. > > Any advice will be welcome. > > -- > Helen > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FUNKM <@t> mercyhealth.com Wed Mar 24 16:02:57 2010 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed Mar 24 16:03:08 2010 Subject: [Histonet] Cover glass In-Reply-To: <2C0FB9C38453F4C9E0B2378A@CDYwxp1931.ad.med.buffalo.edu> References: <3201CF51728F6048A24FA3AFFFEEF1D316BDEA39C2@JHEMTEXVS3.win.ad.jhu.edu> <2C0FB9C38453F4C9E0B2378A@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <4BAA37B1.9B87.00AC.0@mercyhealth.com> Merced, We run 700 to 800 slides per day plus specials and IHC's. We use to wipe down but with staff and workload it was impossible to continue. Thanks for the input and the remember. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> Merced M Leiker 03/24/2010 3:33 PM >>> I use VWR VistaVision coverslips (for fluorescent imaging) and had noticed some dust/debris on them that would autofluoresce and give false positives with some of my stains. The problem disappeared when I got into the habit wiping all my coverslips with EtOH before mounting...this may be cumbersome if you're doing a lot of slides, however. But it has worked really well. Regards, Merced --On Wednesday, March 24, 2010 4:24 PM -0400 Helen Fedor wrote: > Hello, Our lab does IHC staining in house and have had zero problems with > our Fisher brand cover slips until recently. We've tried both Fisher and > Corning cover slips and what appears to be dust or imperfections in the > glass are found on both brands. This is creating false positives for us, > because when the positive staining is low it is difficult to impossible > to tell the imperfections from the staining itself. > > Any advice will be welcome. > > -- > Helen > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adesupo2002 <@t> hotmail.com Wed Mar 24 16:53:12 2010 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Wed Mar 24 16:53:17 2010 Subject: [Histonet] H & E QC Message-ID: Hi, I will appreciate it, if you guys could share your method/procedure for H & E QC with me. Thanking you all for your usual cooperation. Adesupo A. _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_1 From suetp918 <@t> comcast.net Wed Mar 24 18:02:35 2010 From: suetp918 <@t> comcast.net (Sue) Date: Wed Mar 24 18:02:38 2010 Subject: [Histonet] H & E QC In-Reply-To: Message-ID: <1021401464.930521269471755920.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> We have automatic stainers, so after the stainer is set up a slide with a micro-array is run. This slide is checked by the histologists and logged in. The next slides run are our rapid cases. A log sheet is prepared and handed to the pathologist with the slides and they are graded for processing, embedding, microtomy and staining. This log is turned into the supervisor and reviewed daily. If there are issues the supervisor will review with the histologists. Since the techs rotate weekly we are able to monitor all the tech's technical performance. Susan T. Paturzo TJUH From wdesalvo.cac <@t> hotmail.com Wed Mar 24 22:22:34 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Mar 24 22:22:42 2010 Subject: [Histonet] H & E QC In-Reply-To: References: Message-ID: Whether you are using an automated stainer or hand staining, run a control slide and review before any patient samples are stained. I also suggest that only start or endpoint QC is not enough and you should consider incorporating continuous QC/QA at regular intervals for the stain set-up, to ensure the highest quality and provide adequate control of the process. You should be able to determine, in a very short time, the end point of the stain set up and then add QC checks for slide quality at 1/3 and 2/3 through the run or anytime a solution container is changed or rotated. In our lab, with the regents used and staining protocols available to select, we have determined that a stain set of solutions, on our automated instrument, will maintain agreed and desired quality the pathologist will accept for 1500 slides (I strongly suggest counting slides, not runs or racks). We stop processing patient slides and run the control slide at runs 1, 500, 1000 (+- 10% to allow for process flow and variance). The slides are reviewed for acceptance or rejection by a Coordinator or higher and when acceptable,patient slides may be placed on the instrument. All QC slides are saved for review and the QC maintenance sheet is filed daily. This process captures the employee that set up and monitors the instrument and the employee that QC'd along w/ the QC review results. The control slide is a multi-tissue slide that must contain the four highest volume tissue types for the lab. Each pathologist receives a daily Quality Review sheet to report any variance, issues or problems for all cases read. Think through your process, communicate with the pathologist and develop a QC/QA system/process that creates accountability for all employees, supports production of quality results and meets your regulatory needs. William DeSalvo, B.S., HTL(ASCP) System Production Manager Sonora Quest Laboratories NSH Quality Control Committee Chairperson > From: adesupo2002@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 24 Mar 2010 17:53:12 -0400 > Subject: [Histonet] H & E QC > > > Hi, > > I will appreciate it, if you guys could share your method/procedure for H & E QC with me. Thanking you all for your usual cooperation. > > > > Adesupo A. > > _________________________________________________________________ > Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_1_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_1 From annigyg <@t> gmail.com Thu Mar 25 04:34:12 2010 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Mar 25 04:34:18 2010 Subject: [Histonet] number of slides In-Reply-To: References: <306656717.400731269382097568.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: Here is our basic microtomy protocol: BMT - 3 H&Es, each has a ribbon, plus PAS, Retic, Perls Liver bx - 3 H&Es, each has a ribbon plus Retic, Trichrome, Perls, Renal bx - 4 H&Es, each has a short ribbon plus PAS, PMS, trichrome - on slides with gloms Breast bx - 3 H&Es, each has a ribbon Derm bx - 4 H&Es, each has a ribbon, one Path likes AP PAS on all punch bx's GIT bx - 3 H&Es, each has a ribbon, plus HP all other small bx's get 3 H&Es, each with a ribbon and then we wait for the orders for levels and deepers and..and...and AbuDhabiAnnie On 25 March 2010 00:19, hymclab wrote: > We follow the same practice as Susan. Our Pathologists would rather have > more than less!!! > > Dawn > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > Sent: Tuesday, March 23, 2010 5:08 PM > To: anita dudley > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] number of slides > > We do three levels on all diagnostic biopsies. Liver and Kidney also get > upfront special stains, as do gastric biopsies (h pylori) We did cut down on > extra unstained slides since we were discarding most of them. As far as > stopping levels, my pathologist thinks it goes against standard of care. As > for prostate biopsies, they are so small any more, that we are thinking o > cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible > IHC. We are finding that when we have to go back there is minimal tumor for > IHC demonstration. > > Susan T. Paturzo > Thomas Jefferson University Hospital > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may > contain confidential and privileged information for the use of the > designated recipient(s) named above. If you are not the intended recipient, > you are hereby notified that you have received this communication in error > and that any review, disclosure, dissemination, distribution or copying of > it or its contents is prohibited. If you have received this communication in > error, please notify the sender at the electronic mail address noted above > and destroy all copies of this communication and any attachments. Thank you > for your cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From o.isaac24 <@t> yahoo.com Thu Mar 25 05:24:14 2010 From: o.isaac24 <@t> yahoo.com (Isaac O) Date: Thu Mar 25 05:24:19 2010 Subject: [Histonet] LOOKING FOR IHC POSITION Message-ID: <151900.85085.qm@web111613.mail.gq1.yahoo.com> ?Hi, ??? I am?an HTL(ASCP) certified Histotechnologist with many years of experience. I am looking for a new position as IHC Specialist/IHC Supervisor/IHC Tech. ??? Open to relocation. ? Isaac. From malbenatti <@t> googlemail.com Thu Mar 25 06:15:58 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Thu Mar 25 06:16:11 2010 Subject: [Histonet] H & E QC In-Reply-To: References: Message-ID: <41954DF3-4BC0-48E2-83AB-233B613A2DBC@gmail.com> Hi there, HE QC should be carry out on every slides that are stained, if you are checking for the staining intensity, this will be carried out using a control slide on new batch of staining solution, commercial or house made. Batch number should be recorded, and slides labelled with batch reference, date and file accordingly, the same goes for daily QC of routine work. Prior each morning run a control slide is run QC. HE QC goes beyond staining intensity, as a number of artefacts can be introduced and HE unacceptable ( Shatters, creases, folds, scores, sections cut too thick, scams ... to name fews ) and therefore each slides stained should be QC by experience Staff prior been sent out to pathologist, this QC should be recorded and audited on a regular basis. Furthermore over here in the UK every laboratory belong to the UK-NEQAS http://www.ukneqas.org.uk/ and external body that run a number of EQA Schemes for histopathology. For HE EQA for each run the NEQAS will ask to select slides a number of HE slides that were produce at a randomly selected date. Slide will then be send externally and score by external accessor, and benchmark against all the participant in the scheme. If more score too low, lab will be flag and investigated. For those of you in the US, I would be curious to know if you have a similar system, and how they work. Cheers, Malika On 25 Mar 2010, at 03:22, WILLIAM DESALVO wrote: > > Whether you are using an automated stainer or hand staining, run a control slide and review before any patient samples are stained. I also suggest that only start or endpoint QC is not enough and you should consider incorporating continuous QC/QA at regular intervals for the stain set-up, to ensure the highest quality and provide adequate control of the process. You should be able to determine, in a very short time, the end point of the stain set up and then add QC checks for slide quality at 1/3 and 2/3 through the run or anytime a solution container is changed or rotated. > > > > In our lab, with the regents used and staining protocols available to select, we have determined that a stain set of solutions, on our automated instrument, will maintain agreed and desired quality the pathologist will accept for 1500 slides (I strongly suggest counting slides, not runs or racks). We stop processing patient slides and run the control slide at runs 1, 500, 1000 (+- 10% to allow for process flow and variance). The slides are reviewed for acceptance or rejection by a Coordinator or higher and when acceptable,patient slides may be placed on the instrument. All QC slides are saved for review and the QC maintenance sheet is filed daily. This process captures the employee that set up and monitors the instrument and the employee that QC'd along w/ the QC review results. The control slide is a multi-tissue slide that must contain the four highest volume tissue types for the lab. Each pathologist receives a daily Quality Review sheet to report any variance, issues or problems for all cases read. > > > > Think through your process, communicate with the pathologist and develop a QC/QA system/process that creates accountability for all employees, supports production of quality results and meets your regulatory needs. > > William DeSalvo, B.S., HTL(ASCP) > System Production Manager > > Sonora Quest Laboratories > > NSH Quality Control Committee Chairperson > > >> From: adesupo2002@hotmail.com >> To: histonet@lists.utsouthwestern.edu >> Date: Wed, 24 Mar 2010 17:53:12 -0400 >> Subject: [Histonet] H & E QC >>> >> Hi, >> >> I will appreciate it, if you guys could share your method/procedure for H & E QC with me. Thanking you all for your usual cooperation. >> >> >> >> Adesupo A. >> >> _________________________________________________________________ >> Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. >> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_1_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_1_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aplewinski <@t> carisdx.com Thu Mar 25 06:22:11 2010 From: aplewinski <@t> carisdx.com (Plewinski, Amy) Date: Thu Mar 25 06:22:15 2010 Subject: [Histonet] LOOKING FOR IHC POSITION In-Reply-To: <151900.85085.qm@web111613.mail.gq1.yahoo.com> References: <151900.85085.qm@web111613.mail.gq1.yahoo.com> Message-ID: Hi Isaac- I am a corporate recruiter with Caris Life Sciences. Our labs are located in Irving, Texas and Phoenix, AZ and Newton, MA (Boston). Please review our website and contact me back with your resume. www.carislifesciences.com Amy Plewinski aplewinski@carisdx.com 214-364-9271 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Isaac O Sent: Thursday, March 25, 2010 5:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LOOKING FOR IHC POSITION ?Hi, ??? I am?an HTL(ASCP) certified Histotechnologist with many years of experience. I am looking for a new position as IHC Specialist/IHC Supervisor/IHC Tech. ??? Open to relocation. ? Isaac. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Thu Mar 25 08:05:25 2010 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Thu Mar 25 08:05:28 2010 Subject: [Histonet] Symphony Message-ID: <318266.14260.qm@web58606.mail.re3.yahoo.com> I was wondering who out there in Histo Land is using or has used the Ventana Symphony H&E stainer and what their experiences have been. Thanks in advance!! ? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services ? Tele (252)-830-6866 ????????(800)-284-0672 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? ? ? ? From Jackie.O'Connor <@t> abbott.com Thu Mar 25 08:19:41 2010 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Mar 25 08:20:14 2010 Subject: [Histonet] Job Opportunity Message-ID: We have a great full time permanent opportunity for an experienced histotech at Abbott Laboratories in Abbott Park, IL. We are about 40 miles north of Chicago in Lake County. Please go to www.Abbott.com if you are interested in applying for this position. Additionally, if you would like to forward your resume to me, it could be helpful. Jackie O' From ccrowder <@t> vetmed.lsu.edu Thu Mar 25 08:35:53 2010 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Mar 25 08:40:20 2010 Subject: [Histonet] A.O TP8000 Processor Message-ID: Hi - I'm cleaning out my office and found the procedure manual, timing disc and notes for the old A/O TP8000 Processor. If for some reason someone would like these I will mail them to you. Otherwise they are going in the trash. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From christiegowan <@t> msn.com Thu Mar 25 08:46:53 2010 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Mar 25 08:47:05 2010 Subject: [Histonet] Symphony In-Reply-To: <318266.14260.qm@web58606.mail.re3.yahoo.com> References: <318266.14260.qm@web58606.mail.re3.yahoo.com> Message-ID: Kelly, We have had the Symphony since last October and I would be happy to answer any questions you might have. Our experience has been very good as a whole. Let me know what your specific questions are and I will be happy to answer them. > Date: Thu, 25 Mar 2010 06:05:25 -0700 > From: kdboydhisto@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Symphony > > I was wondering who out there in Histo Land is using or has used the Ventana Symphony H&E stainer and what their experiences have been. Thanks in advance!! > > > > Kelly D. Boyd, BS, HTL (ASCP) > Lab Manager > Harris Histology Services > > Tele (252)-830-6866 > (800)-284-0672 > Cell (252)-943-9527 > Fax (252)-830-0032 > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Mar 25 10:06:13 2010 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Mar 25 10:06:19 2010 Subject: [Histonet] Silver Lips and Fingers In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46FAB@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46FAB@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <63C1AA7A-9FAA-4D35-901A-BD1FF80D6041@email.arizona.edu> Beauty mark - like Cindy Crawford has. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Mar 23, 2010, at 9:14 AM, Breeden, Sara wrote: > I'm not a chemist and may shoot myself in the foot here, but if gold > chloride tones silver, would it work on skin? Then you could call > it a > beauty spot? > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From anonwums1 <@t> gmail.com Thu Mar 25 10:43:28 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Mar 25 10:43:37 2010 Subject: [Histonet] Troubleshooting IHC Message-ID: <858249121003250843h2d0f16a2q1a74ad1091630300@mail.gmail.com> Hi all, I've recently run into a problem troubleshooting IHC on mouse bones. I am using the tyramide amplification system using a goat primary, anti-goat biotin, strepavidin-HRP, biotinyl tyramide, followed by another strepavidin-HRP and then DAB+ from Dako. When I use an isotype goat IgG, I get essentially no staining. I titered it once for immunofluorescence (SA-fluor instead of HRP in the last step) and got a titer of 1:800, and these staining conditions are quite reproducible. Then I titered for IHC, and 1:200 gave the best signal to noise. I did a batch of staining using those IHC conditions, and it stained beautifully, comparable to my IF. I then tried to repeat it on a second batch of sections processed in the same way, and the background was terrible (but not on my isotype slide). I thought maybe it was a problem in processing so I did a third batch of sections, and the background was still really bad. I see real staining sometimes, but I need to quantify this staining using histomorphometry, so I really need clean staining. Any ideas? The only thing I can think of is that 1:200 is just at the limit of titration that gives too much background. Thanks, Adam From kenneth.a.troutman <@t> Vanderbilt.Edu Thu Mar 25 10:48:06 2010 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Thu Mar 25 10:48:11 2010 Subject: [Histonet] Benchmark Ultra Message-ID: <7B310892042DA74CB3590053F424CFE60B554173B3@ITS-HCWNEM06.ds.Vanderbilt.edu> Hello, We currently use a Benchmark Ultra and it is worth several XTs. There are some features about it that you should know about before you start a run. What we currently do in our lab is load all of the antibodies we are likely to run every day. This can be troublesome if you run the same 30 antibodies every day (unless you have more than one Ultra, then it is much easier). We currently only run 30 total antibodies on our Ventana platform and of those 30 we run 10 or 12 every day. When we come in, we pull a list of stains for the AM run off the computer and we have a good idea if we need to load something less common along with the list of 12 more common antibodies and a detection kit. For the next run, if we have already started a run and we need to add an antibody, we will be forced to use the "Landing Zone" feature. Using this, we are able to load the missing antibodies/reagents and remove what we don't need. With regard to the "important" or "unimportant" cases, you can flag them with the blue LED, (which looks really cool, by the way...) so that you know visually which cases you are looking for without having to hover over a position on the computer. As far as loading important cases after unimportant cases, I don't think I can offer any advice there. The instrument is essentially 30 individual stainers and you can choose whether or not to load the slides. We don't really encounter this issue because of the way we have structured our workflow, but then again, we have enhanced our workflow based upon the capabilities of the instrumentation that we use. I hope this has helped. If there are any other questions, feel free to email me. Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 Date: Wed, 24 Mar 2010 19:37:25 +0100 From: "Gudrun Lang" Subject: [Histonet] benchmark ultra and continuous workflow To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Benchmark Ultra users! I would like to hear of your experiences with the continuous workflow of this instrument. Does it really make life easier or even more complicated? I think of handling one slide every few minutes after the staining is completed and of the problems, that occur when the stainer is started with "unimportant" cases and the later coming "important" cases have not enough place to complete them in time. Gudrun Lang From NMargaryan <@t> childrensmemorial.org Thu Mar 25 11:07:21 2010 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Mar 25 11:11:15 2010 Subject: [Histonet] coverslipper Message-ID: Hi Colleges, I need your opinion about coverslip by hand vs. using machine. If you use machine what company's coverslipper you prefer? Thanks in advance, Naira From trathborne <@t> somerset-healthcare.com Thu Mar 25 11:14:55 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Mar 25 11:15:01 2010 Subject: [Histonet] coverslipper In-Reply-To: Message-ID: Leica CV5030. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Margaryan, Naira Sent: Thursday, March 25, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslipper Hi Colleges, I need your opinion about coverslip by hand vs. using machine. If you use machine what company's coverslipper you prefer? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From gentras <@t> auburn.edu Thu Mar 25 11:15:44 2010 From: gentras <@t> auburn.edu (Atoska Gentry) Date: Thu Mar 25 11:16:12 2010 Subject: [Histonet] Re: chick embryo frozen sections Message-ID: <4BAB45E0.C676.0026.0@auburn.edu> hello, will some one please give me pointers on successful methods used for cryosectioning bursa & esophagus from approx. 21 day chick embryos (such as recommended temp. & micron thickness, for yielding the best sections)? Thanks! Atoska From gentras <@t> auburn.edu Thu Mar 25 11:21:26 2010 From: gentras <@t> auburn.edu (Atoska Gentry) Date: Thu Mar 25 11:21:42 2010 Subject: [Histonet] Re: Acetone Fixation Message-ID: <4BAB4736.C676.0026.0@auburn.edu> hello, if any of you have acetone fixation incorporated into you frozen section H& E staining protocol will you please advise me on adjustments necessary for routine staining protocol. Also, out of curiosity please enlighten me on the purpose for post sectioning acetone fixation on tissue samples initially fixed in 95% ETOH/ Acetic Acid? Your prompt replies will be greatly appreciated. ~ Atoska From flnails <@t> texaschildrens.org Thu Mar 25 11:31:21 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Mar 25 11:31:36 2010 Subject: [Histonet] RE: coverslipper In-Reply-To: References: Message-ID: It depends on your volume and how long you maintain your slides. Because you are a children's hospital you keep them longer. The Leica brand is very troublesome in high volume situations. I personally prefer Sakura tape for the speed and fast drying time. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, March 25, 2010 11:15 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] coverslipper Leica CV5030. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Margaryan, Naira Sent: Thursday, March 25, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslipper Hi Colleges, I need your opinion about coverslip by hand vs. using machine. If you use machine what company's coverslipper you prefer? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From lpaveli1 <@t> hurleymc.com Thu Mar 25 12:37:48 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Mar 25 12:38:07 2010 Subject: [Histonet] coverslipper In-Reply-To: References: Message-ID: <4BAB672C.59CD.00EE.0@hurleymc.com> We've had our Sakura film coverslipper since '94 and love it. The good........saves us tech time coverslipping for hours each day, plus you can file the slides right away. The bad.....the docs prefer the bone marrow smears to be coverslipped by hand. We have about 12 bone marrows a month........so really....it's not a problem. We've also tried other brands of film, but the cytotechs could notice a refractive difference, so we switched back to the Sakura brand. >>> "Margaryan, Naira" 3/25/2010 12:07 PM >>> Hi Colleges, I need your opinion about coverslip by hand vs. using machine. If you use machine what company's coverslipper you prefer? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Thu Mar 25 12:39:59 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Mar 25 12:40:13 2010 Subject: [Histonet] Re: Acetone Fixation In-Reply-To: <4BAB4736.C676.0026.0@auburn.edu> References: <4BAB4736.C676.0026.0@auburn.edu> Message-ID: <4BAB67AF.59CD.00EE.0@hurleymc.com> What is your goal? A routine H&E or immuno's....etc.?? >>> "Atoska Gentry" 3/25/2010 12:21 PM >>> hello, if any of you have acetone fixation incorporated into you frozen section H& E staining protocol will you please advise me on adjustments necessary for routine staining protocol. Also, out of curiosity please enlighten me on the purpose for post sectioning acetone fixation on tissue samples initially fixed in 95% ETOH/ Acetic Acid? Your prompt replies will be greatly appreciated. ~ Atoska _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Thu Mar 25 12:40:55 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Thu Mar 25 12:41:59 2010 Subject: [Histonet] coverslipper In-Reply-To: References: Message-ID: <000001cacc42$523fa110$f6bee330$@com> Leica CV3050 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, March 25, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslipper Hi Colleges, I need your opinion about coverslip by hand vs. using machine. If you use machine what company's coverslipper you prefer? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpgarcia <@t> salk.edu Thu Mar 25 12:55:56 2010 From: cpgarcia <@t> salk.edu (Carlos G. Perez-Garcia) Date: Thu Mar 25 12:56:46 2010 Subject: [Histonet] whole mount protocol Message-ID: <8D2C6C3B-CCBA-4786-B38E-9F6DE1433095@salk.edu> hi all i am trying to do an in situ hybridization in whole mount brains at P7 but is hard to get staining mostly due to permeabilization issues since is 7 days postnatal brain. I would really appreciate to receive any suggestion or protocol to help me in this issue. Thanks a lot in advance Carlos Carlos G. Perez-Garcia, Ph.D. Molecular Neurobiology Lab (MNL-O) The Salk Institute 10010 North Torrey Pines Road 92037 La Jolla, CA, USA Phone: 858-453-4100 ext. X1449 Fax: 858 558 6207 E-mail: cpgarcia@salk.edu ? From michaels <@t> janelia.hhmi.org Thu Mar 25 13:31:51 2010 From: michaels <@t> janelia.hhmi.org (Susan Michael) Date: Thu Mar 25 13:31:57 2010 Subject: [Histonet] Re: Histonet Digest, Vol 76, Issue 38 In-Reply-To: Message-ID: On 3/25/10 1:04 PM, "histonet-request@lists.utsouthwestern.edu" wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: H & E QC (Sue) > 2. RE: H & E QC (WILLIAM DESALVO) > 3. Re: number of slides (Anne van Binsbergen) > 4. LOOKING FOR IHC POSITION (Isaac O) > 5. Re: H & E QC (Malika Benatti) > 6. RE: LOOKING FOR IHC POSITION (Plewinski, Amy) > 7. Symphony (Kelly Boyd) > 8. Job Opportunity (Jackie M O'Connor) > 9. A.O TP8000 Processor (Cheryl Crowder) > 10. RE: Symphony (CHRISTIE GOWAN) > 11. Re: Silver Lips and Fingers (Andrea Grantham) > 12. Troubleshooting IHC (Adam .) > 13. Benchmark Ultra (Troutman, Kenneth A) > 14. coverslipper (Margaryan, Naira) > 15. RE: coverslipper (Rathborne, Toni) > 16. Re: chick embryo frozen sections (Atoska Gentry) > 17. Re: Acetone Fixation (Atoska Gentry) > 18. RE: coverslipper (Nails, Felton) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 24 Mar 2010 23:02:35 +0000 (UTC) > From: Sue > Subject: Re: [Histonet] H & E QC > To: ADESUPO ADESUYI > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <1021401464.930521269471755920.JavaMail.root@sz0028a.westchester.pa.mail.comca > st.net> > > Content-Type: text/plain; charset=utf-8 > > We have automatic stainers, so after the stainer is set up a slide with a > micro-array is run. This slide is > checked by the histologists and logged in. The next slides run are our rapid > cases. A log sheet is > prepared and handed to the pathologist with the slides and they are graded for > processing, embedding, microtomy > and staining. This log is turned into the supervisor and reviewed daily. If > there are issues the supervisor will > review with the histologists. Since the techs rotate weekly we are able to > monitor all the tech's technical performance. > > Susan T. Paturzo > TJUH > > > > > ------------------------------ > > Message: 2 > Date: Wed, 24 Mar 2010 21:22:34 -0600 > From: WILLIAM DESALVO > Subject: RE: [Histonet] H & E QC > To: , histonet > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Whether you are using an automated stainer or hand staining, run a control > slide and review before any patient samples are stained. I also suggest that > only start or endpoint QC is not enough and you should consider incorporating > continuous QC/QA at regular intervals for the stain set-up, to ensure the > highest quality and provide adequate control of the process. You should be > able to determine, in a very short time, the end point of the stain set up and > then add QC checks for slide quality at 1/3 and 2/3 through the run or anytime > a solution container is changed or rotated. > > > > In our lab, with the regents used and staining protocols available to select, > we have determined that a stain set of solutions, on our automated instrument, > will maintain agreed and desired quality the pathologist will accept for 1500 > slides (I strongly suggest counting slides, not runs or racks). We stop > processing patient slides and run the control slide at runs 1, 500, 1000 (+- > 10% to allow for process flow and variance). The slides are reviewed for > acceptance or rejection by a Coordinator or higher and when acceptable,patient > slides may be placed on the instrument. All QC slides are saved for review and > the QC maintenance sheet is filed daily. This process captures the employee > that set up and monitors the instrument and the employee that QC'd along w/ > the QC review results. The control slide is a multi-tissue slide that must > contain the four highest volume tissue types for the lab. Each pathologist > receives a daily Quality Review sheet to report any variance, issues or > problems for all cases read. > > > > Think through your process, communicate with the pathologist and develop a > QC/QA system/process that creates accountability for all employees, supports > production of quality results and meets your regulatory needs. > > William DeSalvo, B.S., HTL(ASCP) > System Production Manager > > Sonora Quest Laboratories > > NSH Quality Control Committee Chairperson > > >> From: adesupo2002@hotmail.com >> To: histonet@lists.utsouthwestern.edu >> Date: Wed, 24 Mar 2010 17:53:12 -0400 >> Subject: [Histonet] H & E QC >>> >> Hi, >> >> I will appreciate it, if you guys could share your method/procedure for H & E >> QC with me. Thanking you all for your usual cooperation. >> >> >> >> Adesupo A. >> >> _________________________________________________________________ >> Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. >> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL >> :en-US:WM_HMP:032010_1_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL: > en-US:WM_HMP:032010_1 > > ------------------------------ > > Message: 3 > Date: Thu, 25 Mar 2010 13:34:12 +0400 > From: Anne van Binsbergen > Subject: Re: [Histonet] number of slides > To: hymclab > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Here is our basic microtomy protocol: > > BMT - 3 H&Es, each has a ribbon, plus PAS, Retic, Perls > Liver bx - 3 H&Es, each has a ribbon plus Retic, Trichrome, Perls, > Renal bx - 4 H&Es, each has a short ribbon plus PAS, PMS, trichrome - on > slides with gloms > Breast bx - 3 H&Es, each has a ribbon > Derm bx - 4 H&Es, each has a ribbon, one Path likes AP PAS on all punch bx's > GIT bx - 3 H&Es, each has a ribbon, plus HP > > all other small bx's get 3 H&Es, each with a ribbon > > and then we wait for the orders for levels and deepers and..and...and > > AbuDhabiAnnie > > > > On 25 March 2010 00:19, hymclab wrote: > >> We follow the same practice as Susan. Our Pathologists would rather have >> more than less!!! >> >> Dawn >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue >> Sent: Tuesday, March 23, 2010 5:08 PM >> To: anita dudley >> Cc: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] number of slides >> >> We do three levels on all diagnostic biopsies. Liver and Kidney also get >> upfront special stains, as do gastric biopsies (h pylori) We did cut down on >> extra unstained slides since we were discarding most of them. As far as >> stopping levels, my pathologist thinks it goes against standard of care. As >> for prostate biopsies, they are so small any more, that we are thinking o >> cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible >> IHC. We are finding that when we have to go back there is minimal tumor for >> IHC demonstration. >> >> Susan T. Paturzo >> Thomas Jefferson University Hospital >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may >> contain confidential and privileged information for the use of the >> designated recipient(s) named above. If you are not the intended recipient, >> you are hereby notified that you have received this communication in error >> and that any review, disclosure, dissemination, distribution or copying of >> it or its contents is prohibited. If you have received this communication in >> error, please notify the sender at the electronic mail address noted above >> and destroy all copies of this communication and any attachments. Thank you >> for your cooperation. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From NMargaryan <@t> childrensmemorial.org Thu Mar 25 13:16:32 2010 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Mar 25 13:33:02 2010 Subject: [Histonet] RE: coverslipper Message-ID: Thanks a lot to all of you answered me. I was surprise nobody mentioned coverslipper from DAKO. Are any of you have any experience with DAKO's coverslipper? Again Thanks to all, Naira Subject: coverslipper Hi Colleges, I need your opinion about coverslip by hand vs. using machine. If you use machine what company's coverslipper you prefer? Thanks in advance, Naira From malbenatti <@t> googlemail.com Thu Mar 25 14:08:21 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Thu Mar 25 14:08:33 2010 Subject: [Histonet] coverslipper In-Reply-To: <4BAB672C.59CD.00EE.0@hurleymc.com> References: <4BAB672C.59CD.00EE.0@hurleymc.com> Message-ID: We use the Leica CV5030 the advantage of it is that it can be attached to their Auto stainer providing continuous workflow as rack and coverslip 30 slides per run, without the need to physically transfer slides rack between the autostainer and coverslipper or used as a stand alone coverslipper if needed, though at time it can be problematic. For a start glass cover slip must be kept at 37 oC prior use otherwise they tend to stick to each others. Also does not recognised all the slides type unless they are Leica one, or the coverlslipper as been calibrated to use a specific slides type. In the past I used the Sakura Acetate film coverslipper, and their were no major problem with it though remounting section was a very tedious process. Malika On Thu, Mar 25, 2010 at 5:37 PM, Lynette Pavelich wrote: > We've had our Sakura film coverslipper since '94 and love it. The > good........saves us tech time coverslipping for hours each day, plus > you can file the slides right away. The bad.....the docs prefer the > bone marrow smears to be coverslipped by hand. We have about 12 bone > marrows a month........so really....it's not a problem. We've also > tried other brands of film, but the cytotechs could notice a refractive > difference, so we switched back to the Sakura brand. > > >>> "Margaryan, Naira" 3/25/2010 > 12:07 PM >>> > Hi Colleges, > > I need your opinion about coverslip by hand vs. using machine. > If you use machine what company's coverslipper you prefer? > > Thanks in advance, > Naira > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " From jstaruk <@t> masshistology.com Thu Mar 25 14:40:05 2010 From: jstaruk <@t> masshistology.com (jstaruk) Date: Thu Mar 25 14:40:10 2010 Subject: [Histonet] coverslipper In-Reply-To: Message-ID: <0F8EC0652EC34D7DBC8AC47391CF4AD3@JimPC> I purchased a used Hacker RCM-3660 glass coverslipper several years ago. It was missing some parts and the nice people from Hacker supplied me with the needed parts. This work horse has been running 7 days a week for the past several years and has never given us any problems. Great machine and great people. Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malika Benatti Sent: Thursday, March 25, 2010 3:08 PM To: Lynette Pavelich Cc: histonet@lists.utsouthwestern.edu; Naira Margaryan Subject: Re: [Histonet] coverslipper We use the Leica CV5030 the advantage of it is that it can be attached to their Auto stainer providing continuous workflow as rack and coverslip 30 slides per run, without the need to physically transfer slides rack between the autostainer and coverslipper or used as a stand alone coverslipper if needed, though at time it can be problematic. For a start glass cover slip must be kept at 37 oC prior use otherwise they tend to stick to each others. Also does not recognised all the slides type unless they are Leica one, or the coverlslipper as been calibrated to use a specific slides type. In the past I used the Sakura Acetate film coverslipper, and their were no major problem with it though remounting section was a very tedious process. Malika From flnails <@t> texaschildrens.org Thu Mar 25 14:50:45 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Mar 25 14:50:57 2010 Subject: [Histonet] coverslipper In-Reply-To: <0F8EC0652EC34D7DBC8AC47391CF4AD3@JimPC> References: <0F8EC0652EC34D7DBC8AC47391CF4AD3@JimPC> Message-ID: Is this coverslipper now produced by Medite -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Thursday, March 25, 2010 2:40 PM To: malbenatti@gmail.com; 'Lynette Pavelich' Cc: histonet@lists.utsouthwestern.edu; 'Naira Margaryan' Subject: RE: [Histonet] coverslipper I purchased a used Hacker RCM-3660 glass coverslipper several years ago. It was missing some parts and the nice people from Hacker supplied me with the needed parts. This work horse has been running 7 days a week for the past several years and has never given us any problems. Great machine and great people. Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malika Benatti Sent: Thursday, March 25, 2010 3:08 PM To: Lynette Pavelich Cc: histonet@lists.utsouthwestern.edu; Naira Margaryan Subject: Re: [Histonet] coverslipper We use the Leica CV5030 the advantage of it is that it can be attached to their Auto stainer providing continuous workflow as rack and coverslip 30 slides per run, without the need to physically transfer slides rack between the autostainer and coverslipper or used as a stand alone coverslipper if needed, though at time it can be problematic. For a start glass cover slip must be kept at 37 oC prior use otherwise they tend to stick to each others. Also does not recognised all the slides type unless they are Leica one, or the coverlslipper as been calibrated to use a specific slides type. In the past I used the Sakura Acetate film coverslipper, and their were no major problem with it though remounting section was a very tedious process. Malika _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From anonwums1 <@t> gmail.com Thu Mar 25 14:52:32 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Mar 25 14:52:38 2010 Subject: [Histonet] Re: Troubleshooting IHC In-Reply-To: References: Message-ID: <858249121003251252i12d58cecq904fa7a7f5ca8c3f@mail.gmail.com> Yes. I block in 3% H2O2, followed by protein block (it's a mysterious buffer called TNB that comes with the tyramide amplification kit), and then avidin/biotin. Adam On Thu, Mar 25, 2010 at 2:25 PM, Margaryan, Naira < NMargaryan@childrensmemorial.org> wrote: > Hi Adam, > > > > How do you block? > > > > I usually have: H2O2, Avidin/Biotin and Protein blocking steps. > > > > Naira > > > > Message: 12 > > Date: Thu, 25 Mar 2010 10:43:28 -0500 > > From: "Adam ." > > Subject: [Histonet] Troubleshooting IHC > > To: histonet@lists.utsouthwestern.edu > > Message-ID: > > <858249121003250843h2d0f16a2q1a74ad1091630300@mail.gmail.com> > > Content-Type: text/plain; charset=ISO-8859-1 > > > > Hi all, > > > > I've recently run into a problem troubleshooting IHC on mouse bones. I am > > using the tyramide amplification system using a goat primary, anti-goat > > biotin, strepavidin-HRP, biotinyl tyramide, followed by another > > strepavidin-HRP and then DAB+ from Dako. When I use an isotype goat IgG, I > > get essentially no staining. > > > > I titered it once for immunofluorescence (SA-fluor instead of HRP in the > > last step) and got a titer of 1:800, and these staining conditions are > quite > > reproducible. Then I titered for IHC, and 1:200 gave the best signal to > > noise. I did a batch of staining using those IHC conditions, and it stained > > beautifully, comparable to my IF. I then tried to repeat it on a second > > batch of sections processed in the same way, and the background was > terrible > > (but not on my isotype slide). I thought maybe it was a problem in > > processing so I did a third batch of sections, and the background was still > > really bad. I see real staining sometimes, but I need to quantify this > > staining using histomorphometry, so I really need clean staining. Any > ideas? > > The only thing I can think of is that 1:200 is just at the limit of > > titration that gives too much background. > > > > Thanks, > > Adam > > > From ccrowder <@t> vetmed.lsu.edu Thu Mar 25 15:55:11 2010 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Mar 25 15:59:37 2010 Subject: [Histonet] Direction for TP 800 Message-ID: Hi - Got a taker for the directions. Who know? Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From gray <@t> rarc.wisc.edu Thu Mar 25 16:17:21 2010 From: gray <@t> rarc.wisc.edu (Beth A Gray) Date: Thu Mar 25 16:17:25 2010 Subject: [Histonet] primate brain Message-ID: <37BFED01-C892-4F31-A54A-6F73ACFBAB67@rarc.wisc.edu> I have some primate brain blocks to do. When I section the blocks the sections look nice. When I put the ribbon on the water bath( temp is 47) the tissue is wrinkled around the edges. No amount of time on the water makes this better. Ideas to solve this problem would be appreciated. Thanks. From malbenatti <@t> googlemail.com Thu Mar 25 16:30:11 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Thu Mar 25 16:30:17 2010 Subject: [Histonet] primate brain In-Reply-To: <37BFED01-C892-4F31-A54A-6F73ACFBAB67@rarc.wisc.edu> References: <37BFED01-C892-4F31-A54A-6F73ACFBAB67@rarc.wisc.edu> Message-ID: At what thickness do you cut your sections ? For Human brain we cut section at room temperature moist in Molifex and cut sections at 7 ?m for standard HE/ 14 ?m for LFB. Not sure what is the melting point of the wax your use, but if it is around 57 oC you can safely raise the temperature of your water bath to 50 oC. Hope this help. Malika On Thu, Mar 25, 2010 at 9:17 PM, Beth A Gray wrote: > I have some primate brain blocks to do. When I section the blocks the > sections look nice. When I put the ribbon on the water bath( temp is 47) > the tissue is wrinkled around the edges. No amount of time on the water > makes this better. Ideas to solve this problem would be appreciated. > Thanks. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " From etw <@t> gmx.at Fri Mar 26 02:26:23 2010 From: etw <@t> gmx.at (David Santer) Date: Fri Mar 26 02:26:53 2010 Subject: [Histonet] freezing mouse heart tissue Message-ID: <002201caccb5$a448c5e0$ecda51a0$@at> Hello, I am currently trying to produce cryosections from mouse heart tissue. I already have experience with paraffin-sections and had faced no major problems. But with cryosectioning I would ask for your help. You can get an idea of our current status at this link (Hematoxylin test stain, not H&E, the whole heart section looks like this) and as you might guess I am not satisfied with the quality. Would you call this freezing artifacts? Some people suggested that freezing with only LN2 would be not quick enough and create those ice crystals. Here is how we prepared the tissue: After taking out the hearts from the mice, we flushed them retrogradely via the aorta with cold sodium solution. Then we cut the hearts in half, put them into cryomolds and covered them with OCT. Afterwards they were snap-frozen in liquid nitrogen and stored at -80?C. Do you have an advice or maybe a suitable protocol for me? Would you recommend 2-methyl butane? Thank you very much! Greetings from the sunny Vienna! David -- Mit freundlichen Gr??en with kind regards Dr. David Santer Ludwig Boltzmann Cluster for Cardiovascular Research c/o Core Unit for Biomedical Research Waehringer Guertel 18-20 - Leitstelle 1Q A-1090 Vienna Austria Website: www.cardiovascular-research.at From leiker <@t> buffalo.edu Fri Mar 26 08:22:15 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Fri Mar 26 08:22:23 2010 Subject: [Histonet] freezing mouse heart tissue In-Reply-To: <002201caccb5$a448c5e0$ecda51a0$@at> References: <002201caccb5$a448c5e0$ecda51a0$@at> Message-ID: Hi David, I wouldn't snap-freeze the tissue in the OCT blocks. For one this could cause the blocks to crack; you may have noticed this already in some of your blocks? But you may want to try freezing the OCT block by resting the block in a tray of isopentane (2-methylbutane) that has been frozen over liquid N, as you've already thought of. You can also search the Histonet archives on this topic as it's been discussed before here. Regards, Merced --On Friday, March 26, 2010 8:26 AM +0100 David Santer wrote: > Hello, > > > > I am currently trying to produce cryosections from mouse heart tissue. I > already have experience with paraffin-sections and had faced no major > problems. But with cryosectioning I would ask for your help. You can get > an idea of our current status at > this link (Hematoxylin test > stain, not H&E, the whole heart section looks like this) and as you might > guess I am not satisfied with the quality. Would you call this freezing > artifacts? Some people suggested that freezing with only LN2 would be not > quick enough and create those ice crystals. > > > > Here is how we prepared the tissue: > > After taking out the hearts from the mice, we flushed them retrogradely > via the aorta with cold sodium solution. Then we cut the hearts in half, > put them into cryomolds and covered them with OCT. Afterwards they were > snap-frozen in liquid nitrogen and stored at -80?C. > > > > Do you have an advice or maybe a suitable protocol for me? Would you > recommend 2-methyl butane? > > > > Thank you very much! Greetings from the sunny Vienna! > > > > David > > -- > > Mit freundlichen Gr??en > > with kind regards > > > > Dr. David Santer > > > > Ludwig Boltzmann Cluster for Cardiovascular Research > > c/o Core Unit for Biomedical Research > > Waehringer Guertel 18-20 - Leitstelle 1Q > > A-1090 Vienna > > Austria > > > > Website: > www.cardiovascular-research.at > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From malbenatti <@t> googlemail.com Fri Mar 26 08:33:07 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Fri Mar 26 08:33:18 2010 Subject: [Histonet] freezing mouse heart tissue In-Reply-To: References: <002201caccb5$a448c5e0$ecda51a0$@at> Message-ID: You may found that excessive amount of saline could cause freezing artefacts, so dabe Try to embed heart sample on cryostat chuck directly, with OCT then freeze chuck on dry ice block directly as opposed to using liquid nitrogen. Another option, would be freezing tissue in hexane boiling tube, with Acetone/dry ice freezing mixture. Hope this help. Malika On 26 Mar 2010, at 13:22, Merced M Leiker wrote: > Hi David, > > I wouldn't snap-freeze the tissue in the OCT blocks. For one this could cause the blocks to crack; you may have noticed this already in some of your blocks? > > But you may want to try freezing the OCT block by resting the block in a tray of isopentane (2-methylbutane) that has been frozen over liquid N, as you've already thought of. > > You can also search the Histonet archives on this topic as it's been discussed before here. > > Regards, > Merced > > > --On Friday, March 26, 2010 8:26 AM +0100 David Santer wrote: > >> Hello, >> >> >> >> I am currently trying to produce cryosections from mouse heart tissue. I >> already have experience with paraffin-sections and had faced no major >> problems. But with cryosectioning I would ask for your help. You can get >> an idea of our current status at >> this link (Hematoxylin test >> stain, not H&E, the whole heart section looks like this) and as you might >> guess I am not satisfied with the quality. Would you call this freezing >> artifacts? Some people suggested that freezing with only LN2 would be not >> quick enough and create those ice crystals. >> >> >> >> Here is how we prepared the tissue: >> >> After taking out the hearts from the mice, we flushed them retrogradely >> via the aorta with cold sodium solution. Then we cut the hearts in half, >> put them into cryomolds and covered them with OCT. Afterwards they were >> snap-frozen in liquid nitrogen and stored at -80?C. >> >> >> >> Do you have an advice or maybe a suitable protocol for me? Would you >> recommend 2-methyl butane? >> >> >> >> Thank you very much! Greetings from the sunny Vienna! >> >> >> >> David >> >> -- >> >> Mit freundlichen Gr??en >> >> with kind regards >> >> >> >> Dr. David Santer >> >> >> >> Ludwig Boltzmann Cluster for Cardiovascular Research >> >> c/o Core Unit for Biomedical Research >> >> Waehringer Guertel 18-20 - Leitstelle 1Q >> >> A-1090 Vienna >> >> Austria >> >> >> >> Website: >> www.cardiovascular-research.at >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Fri Mar 26 08:49:32 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Mar 26 08:49:38 2010 Subject: [Histonet] elisa's Message-ID: Is there a list serve for elisa's? Margaret Perry From Debora.Probst <@t> crhs.net Fri Mar 26 09:35:01 2010 From: Debora.Probst <@t> crhs.net (Debora Probst) Date: Fri Mar 26 09:33:24 2010 Subject: [Histonet] Coverslippers Message-ID: <4843CFFC8DC9A54E88E4AB3F5A50689816494D79@crhs_exch.nterprise.crhs.net> Yes, I too would like to know if any one has ever used The Dako coverslipper and if they liked it or not. Debora Probst HT Columbus Regional Columbus, Ga. From arvidsonkristen <@t> yahoo.com Fri Mar 26 09:57:57 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Fri Mar 26 09:58:00 2010 Subject: [Histonet] Paraffin Question Message-ID: <921877.33415.qm@web65714.mail.ac4.yahoo.com> How often are people changing/rotating their paraffin?? In other words the dirtiest paraffin is how many days old? From malbenatti <@t> googlemail.com Fri Mar 26 10:06:54 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Fri Mar 26 10:07:07 2010 Subject: [Histonet] Paraffin Question In-Reply-To: <921877.33415.qm@web65714.mail.ac4.yahoo.com> References: <921877.33415.qm@web65714.mail.ac4.yahoo.com> Message-ID: <061F0E9C-2546-46ED-B943-A730D74120DF@gmail.com> This will depend on the amount of blocks processed. I worked in places who used to change their VIP twice a week on Wednesday & Friday, and other that would change processor solutions once a week, but as a rule, processor wax regardless of the make should ALWAYS be changed after a maximum of 5 run for optimal result. Cheers, Malika My current lab On 26 Mar 2010, at 14:57, kristen arvidson wrote: > How often are people changing/rotating their paraffin? In other words the dirtiest paraffin is how many days old? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Mar 26 10:08:03 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Mar 26 10:08:08 2010 Subject: [Histonet] Paraffin Question In-Reply-To: <921877.33415.qm@web65714.mail.ac4.yahoo.com> References: <921877.33415.qm@web65714.mail.ac4.yahoo.com> Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46FBD@nmdamailsvr.nmda.ad.nmsu.edu> I have my processor "set" to notify me at 1500 blocks, at which time I change the paraffins. I usually run 500 in the other solutions (same notification system) before changing. It works for me! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Bonnie.Whitaker <@t> osumc.edu Fri Mar 26 10:08:46 2010 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Fri Mar 26 10:08:59 2010 Subject: [Histonet] FFPE used for EM Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F600166B0A9@msxc06.OSUMC.EDU> Hi Everyone, I hope it's a great Friday for you all. Our renal folks were recently attending a meeting, and they came back with some partial information. (I am hoping some of you might have some additional information.) It seems that one of the lecturers mentioned that some paraffins actually seemed to make a difference in the quality of EM when they had to take tissue from the paraffin blocks. I had never heard of this. I am aware of using Carson's modification of formalin to yield better EM results, but not anything to do with paraffin. Can anyone help me out here? Thanks! Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 From CIngles <@t> uwhealth.org Fri Mar 26 10:17:46 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Fri Mar 26 10:18:41 2010 Subject: [Histonet] PA baby sitter References: <827279.87357.qm@web65715.mail.ac4.yahoo.com> Message-ID: That's OK, our poor PA has to babysit the residents, so she needs all the help she can get!! Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Tue 3/23/2010 5:24 PM To: Rick.Garnhart@memorialhealthsystem.com; Jeffrey Silverman Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PA baby sitter Tell be about it again when you get to gross 38,000 cases/year. Ren? J, --- On Tue, 3/23/10, Jeffrey Silverman wrote: From: Jeffrey Silverman Subject: [Histonet] PA baby sitter To: Rick.Garnhart@memorialhealthsystem.com Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, March 23, 2010, 5:18 PM I had to laugh when I read this one. I'm a PA grossing 8500 surgicals per year in a busy general hospital so it's not just biopsies but lots of major organ resections with tons of orthopedics. I work with two histotechnologists. Not only do I close my own cassettes, I accession most specimens, make my own cassettes, save and dispose of the surgical leftover tissues, serve as histology supervisor and laboratory safety officer for all sections attending all the associated meetings that those two entail, often embed at least half of the tissues and pitch in whenever I'm needed in histo- cutting and running automated specials. Having said that, if there's anyone in Europe looking for such a person and can pay 60K euro per annum , I'd love to meet you. I'm in the opposite boat of Malika. Hey, wanna trade jobs? The techs are at my beck and call to bring me things I need, like more acetone or formalin and/or to attend to whatever other help I need, but no one ever sits with me to close the cassettes and feed me specimens. I agree with the accountability issues involved in lost or mishandled tissue. What happens when the aide is off, do they expect a histotech to come in to close the cassettes for the PA. And the productivity increase for such assistance can't be more than 5% IMHO based on my experience. Jeff Silverman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Fri Mar 26 10:18:36 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Fri Mar 26 10:22:51 2010 Subject: [Histonet] freezing mouse heart tissue In-Reply-To: References: Message-ID: <1B834ACCF142D833D75B1840@CDYwxp1931.ad.med.buffalo.edu> Wow, a whole book has been written on frozen sections? That's fantastic! I've got to get a hold of that! --On Friday, March 26, 2010 8:45 AM -0500 Charles.Scouten@leica-microsystems.com wrote: > > > There is a thorough discussion of these issues in Dr. Peters Book, "A > Practical Guide to Frozen Sections" > > > > > Cordially, > > Charles W. Scouten, Ph.D > > Product Manager, MNL > > Biosystems Division > > > > Leica Biosystems Richmond, Inc. > 5205 Route 12 > P.O. Box 528 > Richmond, IL 60071 > United States of America > > Telephone 630 964 0501 > > facsimile +1 630 964 0576 > > www.MyNeuroLab.com > > www.leica-microsystems.com > > > > IMPORTANT - This email and any attachments may be confidential. Any > retransmissions, dissemination or other use of > > these materials by persons or entities other than the intended recipient > is prohibited. If received in error, please contact > > us and delete all copies. Before opening or using attachments, check them > for viruses and defects. Our liability is limited > > to resupplying any affected attachments. [Any representations or opinions > expressed in this email are those of the > > individual sender]. > > > > > > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malika > Benatti > Sent: Friday, March 26, 2010 8:33 AM > To: Merced M Leiker > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] freezing mouse heart tissue > > > > You may found that excessive amount of saline could cause freezing > artefacts, so dabe > > Try to embed heart sample on cryostat chuck directly, with OCT then > freeze chuck on dry ice block directly as opposed to using liquid > nitrogen. > > Another option, would be freezing tissue in hexane boiling tube, with > Acetone/dry ice freezing mixture. > > Hope this help. > > Malika > > > > On 26 Mar 2010, at 13:22, Merced M Leiker wrote: > >> Hi David, >> >> I wouldn't snap-freeze the tissue in the OCT blocks. For one this could >> cause the blocks to crack; you may have noticed this already in some of >> your blocks? >> >> But you may want to try freezing the OCT block by resting the block in a >> tray of isopentane (2-methylbutane) that has been frozen over liquid N, >> as you've already thought of. >> >> You can also search the Histonet archives on this topic as it's been >> discussed before here. >> >> Regards, >> Merced >> >> >> --On Friday, March 26, 2010 8:26 AM +0100 David Santer < etw@gmx.at> >> wrote: >> >>> Hello, >>> >>> >>> >>> I am currently trying to produce cryosections from mouse heart tissue. >>> I already have experience with paraffin-sections and had faced no >>> major problems. But with cryosectioning I would ask for your help. You >>> can get an idea of our current status at >>> < http://www.d-cup.at/histo/mouseheart.jpg> this link (Hematoxylin test >>> stain, not H&E, the whole heart section looks like this) and as you >>> might guess I am not satisfied with the quality. Would you call this >>> freezing artifacts? Some people suggested that freezing with only LN2 >>> would be not quick enough and create those ice crystals. >>> >>> >>> >>> Here is how we prepared the tissue: >>> >>> After taking out the hearts from the mice, we flushed them retrogradely >>> via the aorta with cold sodium solution. Then we cut the hearts in >>> half, put them into cryomolds and covered them with OCT. Afterwards >>> they were snap-frozen in liquid nitrogen and stored at -80?C. >>> >>> >>> >>> Do you have an advice or maybe a suitable protocol for me? Would you >>> recommend 2-methyl butane? >>> >>> >>> >>> Thank you very much! Greetings from the sunny Vienna! >>> >>> >>> >>> David >>> >>> -- >>> >>> Mit freundlichen Gr??en >>> >>> with kind regards >>> >>> >>> >>> Dr. David Santer >>> >>> >>> >>> Ludwig Boltzmann Cluster for Cardiovascular Research >>> >>> c/o Core Unit for Biomedical Research >>> >>> Waehringer Guertel 18-20 - Leitstelle 1Q >>> >>> A-1090 Vienna >>> >>> Austria >>> >>> >>> >>> Website: < http://www.cardiovascular-research.at> >>> www.cardiovascular-research.at >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> Merced M Leiker >> Research Technician III >> Cardiovascular Medicine >> 348 Biomedical Research Building >> State University of New York at Buffalo >> 3435 Main St, Buffalo, NY 14214 USA >> leiker@buffalo.edu >> 716-829-6118 (Ph) >> 716-829-2665 (Fx) >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From rjbuesa <@t> yahoo.com Fri Mar 26 10:26:53 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 26 10:26:57 2010 Subject: [Histonet] Paraffin Question In-Reply-To: <921877.33415.qm@web65714.mail.ac4.yahoo.com> Message-ID: <228593.67281.qm@web65712.mail.ac4.yahoo.com> You cannot "measure" paraffin changes by dates because it is used as a function of the number of cassettes processed. I always used VIP that have 4 paraffin containers. I used to keep track of the number of cassettes processed daily. When I got to as many cassettes as the VIP was designed to (e.g.: 300 cassettes for VIP 300) I discarded the first paraffin, moved forward?#s 2 and 3, and added new paraffin in #4. By dpoing this the #1 was discarded when the VIP had processed 900 cassettes. Ren? J. --- On Fri, 3/26/10, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] Paraffin Question To: "histonet" Date: Friday, March 26, 2010, 10:57 AM How often are people changing/rotating their paraffin?? In other words the dirtiest paraffin is how many days old? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Fri Mar 26 10:29:09 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Mar 26 10:29:15 2010 Subject: [Histonet] California Histology Society Message-ID: <90354A475B420441B2A0396E5008D49695E83F@copc-sbs.COPC.local> Hi There, Anyone out in histoland know why there's no access to www.californiahistology.org ? I've tried a few times this morning with no luck. I want to send one of my techs to the symposium and need to access the event site. Thanks in advance for any help or explanation. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net From dellav <@t> musc.edu Fri Mar 26 10:52:57 2010 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Mar 26 10:53:01 2010 Subject: [Histonet] RE: Coverslippers In-Reply-To: <4843CFFC8DC9A54E88E4AB3F5A50689816494D79@crhs_exch.nterprise.crhs.net> References: <4843CFFC8DC9A54E88E4AB3F5A50689816494D79@crhs_exch.nterprise.crhs.net> Message-ID: For those who are not aware, the Dako unit is apparently the same coverslipper previously marketed by Surgipath. This information may lead to greater responses since Dako has only recently acquired this unit as I understand it. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debora Probst Sent: Friday, March 26, 2010 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslippers Yes, I too would like to know if any one has ever used The Dako coverslipper and if they liked it or not. Debora Probst HT Columbus Regional Columbus, Ga. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Fri Mar 26 11:05:39 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri Mar 26 11:05:54 2010 Subject: [Histonet] Paraffin Question In-Reply-To: <921877.33415.qm@web65714.mail.ac4.yahoo.com> References: <921877.33415.qm@web65714.mail.ac4.yahoo.com> Message-ID: <4BACA313.59CD.00EE.0@hurleymc.com> I was instructed by a very knowledgeable person in the field that the best processing for your tissue is to change it as often as you change your clearant. So, if you change your clearant after 5 uses, you should also change the parafffin after 5 uses too. Hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 >>> kristen arvidson 3/26/2010 10:57 AM >>> How often are people changing/rotating their paraffin? In other words the dirtiest paraffin is how many days old? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Mar 26 11:08:45 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Mar 26 11:09:23 2010 Subject: [Histonet] RE: Coverslippers In-Reply-To: References: <4843CFFC8DC9A54E88E4AB3F5A50689816494D79@crhs_exch.nterprise.crhs.net>, Message-ID: I saw it at the NSH meeting in Alabama and have been trying to get one ever since. Although I have never had the chance to use it in my lab. It's very small and fast. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie [dellav@musc.edu] Sent: Friday, March 26, 2010 11:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Coverslippers For those who are not aware, the Dako unit is apparently the same coverslipper previously marketed by Surgipath. This information may lead to greater responses since Dako has only recently acquired this unit as I understand it. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debora Probst Sent: Friday, March 26, 2010 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslippers Yes, I too would like to know if any one has ever used The Dako coverslipper and if they liked it or not. Debora Probst HT Columbus Regional Columbus, Ga. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Mar 26 11:09:45 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Mar 26 11:09:52 2010 Subject: [Histonet] California Histology Society In-Reply-To: <90354A475B420441B2A0396E5008D49695E83F@copc-sbs.COPC.local> Message-ID: The server was down. You can also access the site through www.californiahistology.com I can also email you a PDF file of the program Jennifer "Thomas Jasper" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/26/2010 08:32 AM To cc Subject [Histonet] California Histology Society Hi There, Anyone out in histoland know why there's no access to www.californiahistology.org ? I've tried a few times this morning with no luck. I want to send one of my techs to the symposium and need to access the event site. Thanks in advance for any help or explanation. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Fri Mar 26 11:42:38 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Mar 26 11:42:42 2010 Subject: [Histonet] histo techs doing cyto prep work Message-ID: <561894.62023.qm@web54207.mail.re2.yahoo.com> Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? ? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? ? I welcome any and all responses... ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! From cmiller <@t> physlab.com Fri Mar 26 11:59:08 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Mar 26 11:59:16 2010 Subject: [Histonet] looking for employment Message-ID: I am inquiring about employment for my oldest daughter. She is looking for a part time 20 + hours lab assistant position in the Omaha area. She is relocating from CA. She is working on her BSRN and can be flexible with her schedule. The earlier the hours the better. She has some knowledge of Histology and a good work ethic. Thanks Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cgill <@t> marylandgeneral.org Fri Mar 26 12:10:03 2010 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Fri Mar 26 12:10:13 2010 Subject: [Histonet] histo techs doing cyto prep work In-Reply-To: <561894.62023.qm@web54207.mail.re2.yahoo.com> References: <561894.62023.qm@web54207.mail.re2.yahoo.com> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9E8C@MDGEN-EXCH1.marylandgeneral.org> In our lab we (Histo Techs) do the cyto prep work and our supervisor is a cyto tech and HTL she covers both Dept. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Friday, March 26, 2010 12:43 PM To: Histonet Subject: [Histonet] histo techs doing cyto prep work Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? ? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? ? I welcome any and all responses... ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Mar 26 12:10:48 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Mar 26 12:11:00 2010 Subject: [Histonet] histo techs doing cyto prep work In-Reply-To: <561894.62023.qm@web54207.mail.re2.yahoo.com> Message-ID: Cytology preparation was added to the HT (ASCP) exam back in 2001 based on feedback on the number of laboratories that have histotechnicians doing cytology preparation. It was also added as a new chapter in the Carson/Hladik Histotechnology text book. Jennifer MacDonald Kim Tournear Sent by: histonet-bounces@lists.utsouthwestern.edu 03/26/2010 09:45 AM To Histonet cc Subject [Histonet] histo techs doing cyto prep work Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? I welcome any and all responses... ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Fri Mar 26 12:14:14 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri Mar 26 12:14:26 2010 Subject: [Histonet] histo techs doing cyto prep work In-Reply-To: <561894.62023.qm@web54207.mail.re2.yahoo.com> References: <561894.62023.qm@web54207.mail.re2.yahoo.com> Message-ID: <4BACB326.59CD.00EE.0@hurleymc.com> I have worked in union hospital for almost 39 yrs. Back then, there were no cytotechs and the pathologists read out all the cytology slides. As a result, the cytoprep was in our contract to perform. Years later, when we did get cytotechs, they preferred to do all of their prep. 10 years later, they are so swamped, that we are going to take the prep back. As it is in our contract to still perform this task, there is no choice there. In my opinion, ideally, a prep tech should be hired instead. Histotechnology has greatly evolved over the years, and much more is required of us, such as IHC, ISH and more. While it is always good to know these procedures so you can step in if the need arises, a prep tech is a better business choice. In my opinion.....!! Happy Friday, Lynette >>> Kim Tournear 3/26/2010 12:42 PM >>> Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? I welcome any and all responses... ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From malbenatti <@t> googlemail.com Fri Mar 26 12:15:25 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Fri Mar 26 12:15:34 2010 Subject: [Histonet] histo techs doing cyto prep work In-Reply-To: <561894.62023.qm@web54207.mail.re2.yahoo.com> References: <561894.62023.qm@web54207.mail.re2.yahoo.com> Message-ID: Working in Pediatric, without a cytology department, I get opportunity to handle non-gynae cytology sample such as Bronchial Alveolar Lavage, CSF, urine, cyst aspirate blood sample, though all the sample are handle by Registered Biomedical Scientist that have been trained to handle cytological specimen. Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH United Kingdom Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 On 26 Mar 2010, at 16:42, Kim Tournear wrote: > Hi all, > I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? > > Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? > > I welcome any and all responses... > > > > ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) > Histology Supervisor > Tucson Medical Center > Tucson, AZ > ~Don't let your life end before it begins~ > OU Rocks!!!! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy_schmitt <@t> pa-ucl.com Fri Mar 26 12:32:26 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Mar 26 12:32:33 2010 Subject: [Histonet] RE: Histonet Digest, Vol 76, Issue 40 In-Reply-To: <20100326170303.54F3151C98@mail.pa-ucl.com> References: <20100326170303.54F3151C98@mail.pa-ucl.com> Message-ID: <737BD0BF52F0744B96B74B61756AC06441659BF143@hestia.ad.pa-ucl.com> Kim- Our cytoprep area is in with Histology so we are currently training new people coming into the department to do both. We feel it will give us more flexibility with scheduling and just being able to help each other out. I too would be interested in hearing what others are doing. Nancy Schmitt HT(ASCP), MLT(CSMLS) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext.142 nancy_schmitt@pa-ucl.com ------------------------------ Message: 8 Date: Fri, 26 Mar 2010 09:42:38 -0700 (PDT) From: Kim Tournear Subject: [Histonet] histo techs doing cyto prep work To: Histonet Message-ID: <561894.62023.qm@web54207.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? ? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? ? I welcome any and all responses... ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From djohnson <@t> mercedesmedical.com Fri Mar 26 12:33:21 2010 From: djohnson <@t> mercedesmedical.com (Dave Johnson) Date: Fri Mar 26 12:33:52 2010 Subject: [Histonet] looking for employment In-Reply-To: References: Message-ID: Have you checked NSH.org for job postings How about MOHS? They are all short of good techs. Not sure what NE's regs are for Mohs tech requirements but some states I see MT, LPN, RNs do it You can find all mohs docs by state and inquire with each office if they need help http://www.mohssurgery.org/i4a/member_directory/feResultsListing.cfm?dir ectory_id=3 or try http://acms.execinc.com/edibo/SurgeonFinder -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, March 26, 2010 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] looking for employment I am inquiring about employment for my oldest daughter. She is looking for a part time 20 + hours lab assistant position in the Omaha area. She is relocating from CA. She is working on her BSRN and can be flexible with her schedule. The earlier the hours the better. She has some knowledge of Histology and a good work ethic. Thanks Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Fri Mar 26 12:32:47 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Mar 26 12:33:56 2010 Subject: [Histonet] histo techs doing cyto prep work In-Reply-To: <561894.62023.qm@web54207.mail.re2.yahoo.com> References: <561894.62023.qm@web54207.mail.re2.yahoo.com> Message-ID: <000001cacd0a$5a5de370$0f19aa50$@com> That is how I started many moons ago(29 years and counting). I spent 1 week in histology, the next week in cytology, this continued for 2 years. I currently supervise both the histology and the cytology lab sections. My histotechs do little cytology prep work but are willing if time allows to help out at the end of the day. The cyto prep techs are willing to learn histology but there is little time to take advantage of their willingness. Hope this answers your question. Happy Friday Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Friday, March 26, 2010 12:43 PM To: Histonet Subject: [Histonet] histo techs doing cyto prep work Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? ? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? ? I welcome any and all responses... ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Fri Mar 26 13:37:26 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Fri Mar 26 13:37:31 2010 Subject: [Histonet] embedding method for DRGs Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7B53@wlmmsx01.nemours.org> Histonetters- Back to these DRGs again: We are looking for a better way to embedd DRGs from neo-natal mice, for cryotomy.. We normally...using a dissecting scope, remove the DRG from the eppendorph tube with a small spatula from ...touch the drg to some OCT (colored) ....and then touch the OCT to the bottom of a disposable embedding mold (peel-away)...the DRG releases and we fill the mold and move on. But many times we need to "encourage" these neo-natal DRGs off the spatula, with another spatula to get the DRG to release into the OCT. Though this technique works...it is a very time consuming method - and we occassionally do lose one or two of these things, because they are so very very small. We are using marker dye to help us locate after they are on the spatula and getting them from the tube to the spautla AOK, it is getting them from spatula to OCT that is the killer. We are currently drawling off the dye with the point of a Kimwipe and touching the DRG to a frozen dot of OCT, using the cold to capture and hold the DRG on the dot, before finishing the embedding process. This is working slightly more efficiently, but.... Does anyone have a better technique to share? I am losing tech's to "DRG blindness"!...It's like trying to embedd a speck of dust...and everyone hates to do them. This is the only technique I have ever used....but always on full term mice..No problem. It works great; but on the neo-natal mice....well you all get the picture. Too teeny tiny.....frustrating, and takes a two man team to do! Ever so time consuming to do! PS: histo-gel doesn't not work, either. From Bonnie.Whitaker <@t> osumc.edu Fri Mar 26 16:08:03 2010 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Fri Mar 26 16:08:15 2010 Subject: [Histonet] Histotech position in Little Rock Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F600166B0AB@msxc06.OSUMC.EDU> Hi Everyone, I hear that there is a histotech position open at University of Arkansas Medical Center. If you are interested, go online and apply! There are some great folks in Arkansas! I'm sure it will be a great opportunity for the right person. Bonnie Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 From Traczyk7 <@t> aol.com Fri Mar 26 18:25:24 2010 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Fri Mar 26 18:25:44 2010 Subject: [Histonet] coverslipper Message-ID: <79923.101a364d.38de9c64@aol.com> Hacker Instruments is still the "go-to" company for new or used RCM and HCM style coverslippers. Dorothy In a message dated 3/25/2010 3:51:13 P.M. Eastern Daylight Time, flnails@texaschildrens.org writes: Is this coverslipper now produced by Medite -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Thursday, March 25, 2010 2:40 PM To: malbenatti@gmail.com; 'Lynette Pavelich' Cc: histonet@lists.utsouthwestern.edu; 'Naira Margaryan' Subject: RE: [Histonet] coverslipper I purchased a used Hacker RCM-3660 glass coverslipper several years ago. It was missing some parts and the nice people from Hacker supplied me with the needed parts. This work horse has been running 7 days a week for the past several years and has never given us any problems. Great machine and great people. Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malika Benatti Sent: Thursday, March 25, 2010 3:08 PM To: Lynette Pavelich Cc: histonet@lists.utsouthwestern.edu; Naira Margaryan Subject: Re: [Histonet] coverslipper We use the Leica CV5030 the advantage of it is that it can be attached to their Auto stainer providing continuous workflow as rack and coverslip 30 slides per run, without the need to physically transfer slides rack between the autostainer and coverslipper or used as a stand alone coverslipper if needed, though at time it can be problematic. For a start glass cover slip must be kept at 37 oC prior use otherwise they tend to stick to each others. Also does not recognised all the slides type unless they are Leica one, or the coverlslipper as been calibrated to use a specific slides type. In the past I used the Sakura Acetate film coverslipper, and their were no major problem with it though remounting section was a very tedious process. Malika _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Mar 27 13:18:09 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Mar 27 13:18:14 2010 Subject: [Histonet] Re: histo techs doing cyto prep work Message-ID: The Pap stain needs frequent tweaking to keep the quality of staining optimal. If the cytotechnologists don't do it themselves, they need to be able to communicate easily with the people who do the cytopreparation for them, and the people who do the cytopreparation need to be able to assess the adequacy of the stain (yes, that means looking down a microscope). I've worked in at least two labs where this feedback wasn't done. In one case, it was probably the cause of some successful litigation against the lab. Good mushroom management, but bad medicine and bad CYA. Bob Richmond Samurai Pathologist Knoxville TN From saby_joseph_a <@t> yahoo.com Sat Mar 27 19:03:53 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Sat Mar 27 19:04:00 2010 Subject: [Histonet] mouse perfusion rate In-Reply-To: <8A369CED96A71BED89D360E5@CDYwxp1931.ad.med.buffalo.edu> References: <8A369CED96A71BED89D360E5@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <360010.50186.qm@web113814.mail.gq1.yahoo.com> All- From previous work with rat perfusions, the flow rate was about 10 ml/minute.? If I had to guess, the equivalent flow rate for a mouse would be closer to 1-3 mls/10 minutes.? If you go 10 ml/minute, you will definitely cause blowout artefacts. Joe Saby, BA HT ________________________________ From: Merced M Leiker To: Charles.Scouten@leica-microsystems.com; making@ufl.edu; histonet@lists.utsouthwestern.edu Sent: Fri, March 19, 2010 9:21:38 AM Subject: RE: [Histonet] mouse perfusion rate The vasculature will leak too much and the mouse will get bloated - you'll see it first in either the intestines blowing up like a balloon or fluid coming out of the nose. Just not the same as the heart pumping when the mouse is alive with intact physiology and normal functioning.? Don't know exactly why, but that's what happens when you go too fast.? Perhaps the vasculature has lost its control to compensate for the pressure? I'm not a physiologist so I'm not sure why...maybe someone on the Histonet can answer that? Regards, Merced --On Thursday, March 18, 2010 5:49 PM -0500 Charles.Scouten@leica-microsystems.com wrote: > > > Why not?? What happens?? One would think the mammalian cardiovascular > system could withstand physiological pressures and flow rates, at least > for one lifetime? > > > > > Cordially, > > Charles W. Scouten, Ph.D > > Product Manager, MNL > > Biosystems Division > > > > Leica Biosystems Richmond, Inc. > 5205 Route 12 > P.O. Box 528 > Richmond, IL 60071 > United States of America > > Telephone 630 964 0501 > > facsimile +1 630 964 0576 > > www.MyNeuroLab.com > > www.leica-microsystems.com > > > > IMPORTANT - This email and any attachments may be confidential. Any > retransmissions, dissemination or other use of > > these materials by persons or entities other than the intended recipient > is prohibited. If received in error, please contact > > us and delete all copies. Before opening or using attachments, check them > for viruses and defects. Our liability is limited > > to resupplying any affected attachments. [Any representations or opinions > expressed in this email are those of the > > individual sender]. > > > > > > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M > Leiker > Sent: Thursday, March 18, 2010 12:38 PM > To: MKing ; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] mouse perfusion rate > > > > That may be mouse cardiac output, but I can assure you, from experience, > you do not want to perfuse at 17ml/min. > > Regards, > Merced > > --On Thursday, March 18, 2010 1:32 PM -0400 MKing < making@ufl.edu> > wrote: > >> Li, >> >> Mouse cardiac output seems to be about 17 ml/min (e.g. >> www.transonic.com/mice1.shtml), you probably want to try for that to >> keep? pressures close to physiological. >> A syringe pump is pretty inexpensive and probably all you need. >> >> Mike >> >> ----- Original Message ----- >> From: Li Zhang < dancingwing@yahoo.com> >> Date: Wednesday, March 17, 2010 14:59 >> Subject: [Histonet] question about mouse perfusion >> To: histonet@lists.utsouthwestern.edu >> >> > > My question is: can anyone give me a rough idea of how fast I >> > > should inject ( like ml/min). I think I've tried like 30 ml in 3 >> > > min, and I suspect that it's too fast because I do observe >> > > tissue swelling sometimes. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214? USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbass <@t> wfubmc.edu Sun Mar 28 12:23:52 2010 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Sun Mar 28 12:23:56 2010 Subject: [Histonet] Used knife sharpeners Message-ID: Hey Guys, What?s the most convenient and economical way to sharpen a knife for an AO860? I know there are commercial services you can send the knife to. I?ve also seen used knife sharpeners on ebay. Since my AO microtome is so nice, I?m tempted to get one of the AO knife sharpeners (like the 935). Any recommendations on what to buy or look for in a used knife sharpener? Any recommendations for a book or website on basic knife sharpening techniques? Thanks, Caroline Bass From rjbuesa <@t> yahoo.com Sun Mar 28 14:14:57 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Mar 28 14:15:00 2010 Subject: [Histonet] Used knife sharpeners In-Reply-To: Message-ID: <96949.82079.qm@web65704.mail.ac4.yahoo.com> This is the same of decision between "renting a house" or "owning a house". If the sharpener is in good condition and you can get it cheap, I would go with "owning it" and getting as many knives as I can so I can have always have a newly sharpened one every time. Ren? J. --- On Sun, 3/28/10, Caroline Bass wrote: From: Caroline Bass Subject: [Histonet] Used knife sharpeners To: "histonet@lists.utsouthwestern.edu" Date: Sunday, March 28, 2010, 1:23 PM Hey Guys, What?s the most convenient and economical way to sharpen a knife for an AO860? I know there are commercial services you can send the knife to. I?ve also seen used knife sharpeners on ebay. Since my AO microtome is so nice, I?m tempted to get one of the AO knife sharpeners (like the 935). Any recommendations on what to buy or look for in a used knife sharpener? Any recommendations for a book or website on basic knife sharpening techniques? Thanks, Caroline Bass _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Sun Mar 28 17:40:06 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Mar 28 17:40:23 2010 Subject: [Histonet] freezing mouse heart tissue In-Reply-To: <002201caccb5$a448c5e0$ecda51a0$@at> Message-ID: David, Two ideas: My suspician is that this might not be ice-crystal artifact. I have seen similar if the frozen tissue has been stored at -20oC instead of -70oC. What happens is the tissue begins to dessicate (like sausage stored uncovered in the fridge). There was a recent article in the Journal of Histotechnology that might save the day: Iren Horkayne-Szakaly; Glenn D. Sandberg; Joren Keylock; Elisabeth J. Rushing (2009) "Nonfrozen Transport Medium Preserves and Restores Skeletal Muscle Enzymatic Activity and Morphology" J Histotechnol 32(2):49-53 Though I notice that you perfuse with a cold sodium solution. Again since saline is not isotonic with cells, water tends to be dragged into the cells in order to equalise the salt concentrations, causing the cells to swell. When the tissue is frozen, large ice crystals result. See: Henwood, A., (2007) "Adverse effect of saline on brain intraoperative (frozen section) Histology" J Histotechnol 30(3):193. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Santer Sent: Friday, 26 March 2010 6:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] freezing mouse heart tissue Hello, I am currently trying to produce cryosections from mouse heart tissue. I already have experience with paraffin-sections and had faced no major problems. But with cryosectioning I would ask for your help. You can get an idea of our current status at this link (Hematoxylin test stain, not H&E, the whole heart section looks like this) and as you might guess I am not satisfied with the quality. Would you call this freezing artifacts? Some people suggested that freezing with only LN2 would be not quick enough and create those ice crystals. Here is how we prepared the tissue: After taking out the hearts from the mice, we flushed them retrogradely via the aorta with cold sodium solution. Then we cut the hearts in half, put them into cryomolds and covered them with OCT. Afterwards they were snap-frozen in liquid nitrogen and stored at -80?C. Do you have an advice or maybe a suitable protocol for me? Would you recommend 2-methyl butane? Thank you very much! Greetings from the sunny Vienna! David -- Mit freundlichen Gr??en with kind regards Dr. David Santer Ludwig Boltzmann Cluster for Cardiovascular Research c/o Core Unit for Biomedical Research Waehringer Guertel 18-20 - Leitstelle 1Q A-1090 Vienna Austria Website: www.cardiovascular-research.at _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From jkiernan <@t> uwo.ca Mon Mar 29 00:22:21 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Mar 29 00:22:26 2010 Subject: [Histonet] embedding method for DRGs Message-ID: Dear Carol Barone, You are asking the wrong group (Histonet), and almost anonymously. Your email address indicates that you work for a BIG company. Your employer should send you on a course to learn how to do microdissection of fetal and neonatal mice. You may get some free advice from listservers, but how will you know if it's any good? The skills you ask about need expert hands-on teaching, such as a few months in a lab that does that kind of work. John A. Kiernan MB, ChB, PhD, DSc Professor, Dept of Anatomy & Cell Biology The University of Western Ontario LONDON, Canada N6A 5C1 Phone: (519) 679-2111 x 86822 FAX: (519) 661-3936 http://biostain.com http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/ = = = ----- Original Message ----- From: "Barone, Carol " Date: Friday, March 26, 2010 14:38 Subject: [Histonet] embedding method for DRGs To: histonet@lists.utsouthwestern.edu > Histonetters- Back to these DRGs again: > > We are looking for a better way to embedd DRGs from neo-natal > mice, for > cryotomy.. We normally...using a dissecting scope, remove the > DRG from > the eppendorph tube with a small spatula from ...touch the drg > to some > OCT (colored) ....and then touch the OCT to the bottom of a disposable > embedding mold (peel-away)...the DRG releases and we fill the > mold and > move on. > > But many times we need to "encourage" these neo-natal DRGs off the > spatula, with another spatula to get the DRG to release into the OCT. > Though this technique works...it is a very time consuming method > - and > we occassionally do lose one or two of these things, because > they are so > very very small. We are using marker dye to help us locate > after they > are on the spatula and getting them from the tube to the spautla > AOK, it > is getting them from spatula to OCT that is the killer. > > We are currently drawling off the dye with the point of a > Kimwipe and > touching the DRG to a frozen dot of OCT, using the cold to > capture and > hold the DRG on the dot, before finishing the embedding process. > This is working slightly more efficiently, but.... > > Does anyone have a better technique to share? I am losing tech's > to "DRG > blindness"!...It's like trying to embedd a speck of dust...and > everyonehates to do them. This is the only technique I have ever > used....butalways on full term mice..No problem. It works great; > but on the > neo-natal mice....well you all get the picture. Too teeny > tiny.....frustrating, and takes a two man team to do! Ever so time > consuming to do! > > PS: histo-gel doesn't not work, either. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Traczyk7 <@t> aol.com Mon Mar 29 08:04:18 2010 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Mon Mar 29 08:04:49 2010 Subject: [Histonet] Used knife sharpeners Message-ID: <5fc5e.26f7d318.38e1ff52@aol.com> Caroline, You can buy a new or refurbished HI-76 knife sharpener thru Hacker Instruments and it will come with a warranty. If you find one on Ebay, you can get the replacement honing and stropping wheels from Hacker. ( I know, it's quite the name for a company that sells knife sharpeners). The HI-76 comes with an instructional video. It's the one thing that is usually missing when you acquire used equipment. The guy you need to talk to at Hacker is Jim Mullen 800-442-2537. Good luck with your search. Dorothy Dorothy Traczyk MTA Histology LLC PO Box 602 Point Pleasant, NJ 08742 T: 732-899-2912 F: 732-899-5469 www.mtahistology.com In a message dated 3/28/2010 1:24:21 P.M. Eastern Daylight Time, cbass@wfubmc.edu writes: Hey Guys, What?s the most convenient and economical way to sharpen a knife for an AO860? I know there are commercial services you can send the knife to. I?ve also seen used knife sharpeners on ebay. Since my AO microtome is so nice, I?m tempted to get one of the AO knife sharpeners (like the 935). Any recommendations on what to buy or look for in a used knife sharpener? Any recommendations for a book or website on basic knife sharpening techniques? Thanks, Caroline Bass _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dreynold <@t> mdanderson.org Mon Mar 29 08:57:41 2010 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Mon Mar 29 08:57:50 2010 Subject: [Histonet] RE:cover slips In-Reply-To: <5037c7ac-032d-4ca9-b667-86051f255fb4@DCPWPRTR01.mdanderson.edu> References: <5037c7ac-032d-4ca9-b667-86051f255fb4@DCPWPRTR01.mdanderson.edu> Message-ID: <785BBF0C5F49CE41BA74460A43A08F02167482BEA5@DCPWVMBXC0VS3.mdanderson.edu> I have tried several brands all have the same problem some worse than others. But even worse than the coverslips are the slides. I cut a lot of frozen sections on charged slides for fluorescent labeling the slides are dirty and the dirt gets trapped under the tissue as well as around it. The dirt is auto fluorescent. Sometimes it is hard to tell label from auto fluorescence. I have tried slides from multiple vendors and all seem to have the same problem. Does anyone have a good vendor for clean charged slides. Message: 15 Date: Wed, 24 Mar 2010 16:24:18 -0400 From: Helen Fedor Subject: [Histonet] Cover glass To: "histonet@lists.utsouthwestern.edu" Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D316BDEA39C2@JHEMTEXVS3.win.ad.jhu.edu> Content-Type: text/plain; charset="us-ascii" Hello, Our lab does IHC staining in house and have had zero problems with our Fisher brand cover slips until recently. We've tried both Fisher and Corning cover slips and what appears to be dust or imperfections in the glass are found on both brands. This is creating false positives for us, because when the positive staining is low it is difficult to impossible to tell the imperfections from the staining itself. Any advice will be welcome. -- Helen ******************* From leiker <@t> buffalo.edu Mon Mar 29 09:05:03 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Mar 29 09:05:13 2010 Subject: [Histonet] mouse perfusion rate In-Reply-To: <360010.50186.qm@web113814.mail.gq1.yahoo.com> References: <8A369CED96A71BED89D360E5@CDYwxp1931.ad.med.buffalo.edu> <360010.50186.qm@web113814.mail.gq1.yahoo.com> Message-ID: Hi Joe, Thanks for that notice about flow rates. But I think for the mouse you meant 1-3mls/min (not per 10min?)... Regards, Merced --On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby wrote: > > > All- > > From previous work with rat perfusions, the flow rate was about 10 > ml/minute. If I had to guess, the equivalent flow rate for a mouse would > be closer to 1-3 mls/10 minutes. If you go 10 ml/minute, you will > definitely cause blowout artefacts. > > Joe Saby, BA HT > > > > > __________________________________________________ > From: Merced M Leiker > To: Charles.Scouten@leica-microsystems.com; making@ufl.edu; > histonet@lists.utsouthwestern.edu > Sent: Fri, March 19, 2010 9:21:38 AM > Subject: RE: [Histonet] mouse perfusion rate > > The vasculature will leak too much and the mouse will get bloated - > you'll > see it first in either the intestines blowing up like a balloon or fluid > coming out of the nose. Just not the same as the heart pumping when the > mouse is alive with intact physiology and normal functioning. Don't know > exactly why, but that's what happens when you go too fast. Perhaps the > vasculature has lost its control to compensate for the pressure? I'm not > a > physiologist so I'm not sure why...maybe someone on the Histonet can > answer > that? > > Regards, > Merced > > --On Thursday, March 18, 2010 5:49 PM -0500 > Charles.Scouten@leica-microsystems.com wrote: > >> >> >> Why not? What happens? One would think the mammalian cardiovascular >> system could withstand physiological pressures and flow rates, at least >> for one lifetime? >> >> >> >> >> Cordially, >> >> Charles W. Scouten, Ph.D >> >> Product Manager, MNL >> >> Biosystems Division >> >> >> >> Leica Biosystems Richmond, Inc. >> 5205 Route 12 >> P.O. Box 528 >> Richmond, IL 60071 >> United States of America >> >> Telephone 630 964 0501 >> >> facsimile +1 630 964 0576 >> >> www.MyNeuroLab.com >> >> www.leica-microsystems.com >> >> >> >> IMPORTANT - This email and any attachments may be confidential. Any >> retransmissions, dissemination or other use of >> >> these materials by persons or entities other than the intended recipient >> is prohibited. If received in error, please contact >> >> us and delete all copies. Before opening or using attachments, check them >> for viruses and defects. Our liability is limited >> >> to resupplying any affected attachments. [Any representations or opinions >> expressed in this email are those of the >> >> individual sender]. >> >> >> >> >> >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M >> Leiker >> Sent: Thursday, March 18, 2010 12:38 PM >> To: MKing ; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] mouse perfusion rate >> >> >> >> That may be mouse cardiac output, but I can assure you, from experience, >> you do not want to perfuse at 17ml/min. >> >> Regards, >> Merced >> >> --On Thursday, March 18, 2010 1:32 PM -0400 MKing < making@ufl.edu> >> wrote: >> >>> Li, >>> >>> Mouse cardiac output seems to be about 17 ml/min (e.g. >>> www.transonic.com/mice1.shtml), you probably want to try for that to >>> keep pressures close to physiological. >>> A syringe pump is pretty inexpensive and probably all you need. >>> >>> Mike >>> >>> ----- Original Message ----- >>> From: Li Zhang < dancingwing@yahoo.com> >>> Date: Wednesday, March 17, 2010 14:59 >>> Subject: [Histonet] question about mouse perfusion >>> To: histonet@lists.utsouthwestern.edu >>> >>> > > My question is: can anyone give me a rough idea of how fast I >>> > > should inject ( like ml/min). I think I've tried like 30 ml in 3 >>> > > min, and I suspect that it's too fast because I do observe >>> > > tissue swelling sometimes. >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> Merced M Leiker >> Research Technician III >> Cardiovascular Medicine >> 348 Biomedical Research Building >> State University of New York at Buffalo >> 3435 Main St, Buffalo, NY 14214 USA >> leiker@buffalo.edu >> 716-829-6118 (Ph) >> 716-829-2665 (Fx) >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> ______________________________________________________________________ >> This email has been scanned by the MessageLabs Email Security System. >> For more information please visit http://www.messagelabs.com/email >> ______________________________________________________________________ > > > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From kcrosby <@t> cellsignal.com Mon Mar 29 07:55:33 2010 From: kcrosby <@t> cellsignal.com (Katie Crosby) Date: Mon Mar 29 09:06:51 2010 Subject: [Histonet] Removing tissues from OCT for molecular analysis Message-ID: <7A4D7141-2408-42D4-94A6-8E606C498142@cellsignal.com> Hello, Can someone suggest a means of removing tissues from OCT for subsequent molecular analysis? I did not find anything in the archives describing this exactly. Thanks, Katie Katie Crosby Immunohistochemistry Cell Signaling Technology 3 Trask Lane Danvers, MA 01923 Phone: 978-867-2352 Fax: 978-867-2400 WEB Site: www.cellsignal.com Toll Free: 1-877-616-CELL (877-616-2355) 1-877-678-TECH (877-678-8324) From relia1 <@t> earthlink.net Mon Mar 29 09:16:17 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Mar 29 09:17:04 2010 Subject: [Histonet] RELIA Special Job Alert for Managers and Supervisors 3-29-10 Message-ID: Hi Histonetters!, I have several exciting opportunities for experienced Managers, and Supervisors in hospital and private lab environments in several locations nationwide. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clients offer excellent compensation, benefits and relocation assistance. Here are my leadership positions: Histology Manager - Long Island, NY NYS lic NOT req Lab Manager - Uniondale, NY Lab Manager - Tempe, AZ Lab Manager - Richmond,VA Histology Manager - Uniondale, NY Histology Manager - Tempe, AZ Histology Manager - Richmond,VA Histology Supervisor - Las Vegas, NV Histology Manager - Spokane, WA If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 6 years I have dedicated my practice solely to placing histology professionals like you. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia From malbenatti <@t> googlemail.com Mon Mar 29 09:43:07 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Mon Mar 29 09:43:09 2010 Subject: [Histonet] Removing tissues from OCT for molecular analysis In-Reply-To: <19ED2B54-9CA9-49FA-AE43-C6847A906040@cellsignal.com> References: <7A4D7141-2408-42D4-94A6-8E606C498142@cellsignal.com> <3E1495E6-23C1-4F8B-BB2D-4E5A9C7D0216@gmail.com> <19ED2B54-9CA9-49FA-AE43-C6847A906040@cellsignal.com> Message-ID: What you should do ideally is sample you tissue for molecular studie first, then carry out frozen sections Malika " ... Smile it confuses people ..." On 29 Mar 2010, at 15:31, Katie Crosby wrote: > I would like to remove tissues from blocks of OCT and perform > molecule studies. I need to separate tissues from OCT. > Thanks, > Katie > > On Mar 29, 2010, at 10:21 AM, Malika Benatti wrote: > >> Do you mean removing OCT from tissue ? >> >> " ... Smile it confuses people ..." >> >> On 29 Mar 2010, at 13:55, Katie Crosby >> wrote: >> >>> Hello, >>> >>> Can someone suggest a means of removing tissues from OCT for >>> subsequent molecular analysis? I did not find anything in the >>> archives describing this exactly. >>> >>> Thanks, >>> >>> Katie >>> >>> >>> >>> >>> >>> >>> Katie Crosby >>> Immunohistochemistry >>> Cell Signaling Technology >>> 3 Trask Lane >>> Danvers, MA 01923 >>> >>> Phone: 978-867-2352 >>> Fax: 978-867-2400 >>> WEB Site: www.cellsignal.com >>> >>> Toll Free: >>> 1-877-616-CELL (877-616-2355) >>> 1-877-678-TECH (877-678-8324) >>> >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > Katie Crosby > Immunohistochemistry > Cell Signaling Technology > 3 Trask Lane > Danvers, MA 01923 > > Phone: 978-867-2352 > Fax: 978-867-2400 > WEB Site: www.cellsignal.com > > Toll Free: > 1-877-616-CELL (877-616-2355) > 1-877-678-TECH (877-678-8324) > > > From Charles.Scouten <@t> leica-microsystems.com Mon Mar 29 13:26:58 2010 From: Charles.Scouten <@t> leica-microsystems.com (Charles.Scouten@leica-microsystems.com) Date: Mon Mar 29 13:26:39 2010 Subject: [Histonet] mouse perfusion rate Message-ID: I have perfused mice and rats at 300 mm Hg, about double physiological level, don't know what that made the flow rate. All mammals have the same blood pressure (within tolerances), so it is easier to select a suitable pressure to use than a flow rate, which varies dramatically. I look at brain, never pay any attention to the gut. Clear fluid comes out the nose, that is a good sign. There are pressure release valves across the cribiform plate to release CSF if there is too much. I am flooding the system, fluid coming out the nose means the extracellular fluid and CSF is being replaced as well as vascular blood. Good. The tissue is quality is excellent, free of red blood cells, can be unshrunk depending on the tonicity (should be sub isotonic) of the fixative fluid. Have looked at Nissl and EM material, no evidence of damage to the tissue. If gut is extended, might have something to do with the large intestines job of removing fluid from feces, and flooding the system swells the tissue. But does it matter? Do you use that tissue? What is the tissue quality if you use it after physiological pressure perfusion. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: Merced M Leiker [mailto:Merced M Leiker ] Sent: Monday, March 29, 2010 9:05 AM To: Joseph Saby ; Charles.Scouten@leica-microsystems.com; making@ufl.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mouse perfusion rate Hi Joe, Thanks for that notice about flow rates. But I think for the mouse you meant 1-3mls/min (not per 10min?)... Regards, Merced --On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby < saby_joseph_a@yahoo.com> wrote: > > > All- > > From previous work with rat perfusions, the flow rate was about 10 > ml/minute. If I had to guess, the equivalent flow rate for a mouse would > be closer to 1-3 mls/10 minutes. If you go 10 ml/minute, you will > definitely cause blowout artefacts. > > Joe Saby, BA HT > > > > > __________________________________________________ > From: Merced M Leiker < leiker@buffalo.edu> > To: Charles.Scouten@leica-microsystems.com; making@ufl.edu; > histonet@lists.utsouthwestern.edu > Sent: Fri, March 19, 2010 9:21:38 AM > Subject: RE: [Histonet] mouse perfusion rate > > The vasculature will leak too much and the mouse will get bloated - > you'll > see it first in either the intestines blowing up like a balloon or fluid > coming out of the nose. Just not the same as the heart pumping when the > mouse is alive with intact physiology and normal functioning. Don't know > exactly why, but that's what happens when you go too fast. Perhaps the > vasculature has lost its control to compensate for the pressure? I'm not > a > physiologist so I'm not sure why...maybe someone on the Histonet can > answer > that? > > Regards, > Merced > > --On Thursday, March 18, 2010 5:49 PM -0500 > Charles.Scouten@leica-microsystems.com wrote: > >> >> >> Why not? What happens? One would think the mammalian cardiovascular >> system could withstand physiological pressures and flow rates, at least >> for one lifetime? >> >> >> >> >> Cordially, >> >> Charles W. Scouten, Ph.D >> >> Product Manager, MNL >> >> Biosystems Division >> >> >> >> Leica Biosystems Richmond, Inc. >> 5205 Route 12 >> P.O. Box 528 >> Richmond, IL 60071 >> United States of America >> >> Telephone 630 964 0501 >> >> facsimile +1 630 964 0576 >> >> www.MyNeuroLab.com >> >> www.leica-microsystems.com >> >> >> >> IMPORTANT - This email and any attachments may be confidential. Any >> retransmissions, dissemination or other use of >> >> these materials by persons or entities other than the intended recipient >> is prohibited. If received in error, please contact >> >> us and delete all copies. Before opening or using attachments, check them >> for viruses and defects. Our liability is limited >> >> to resupplying any affected attachments. [Any representations or opinions >> expressed in this email are those of the >> >> individual sender]. >> >> >> >> >> >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M >> Leiker < leiker@buffalo.edu> >> Sent: Thursday, March 18, 2010 12:38 PM >> To: MKing < making@ufl.edu>; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] mouse perfusion rate >> >> >> >> That may be mouse cardiac output, but I can assure you, from experience, >> you do not want to perfuse at 17ml/min. >> >> Regards, >> Merced >> >> --On Thursday, March 18, 2010 1:32 PM -0400 MKing < making@ufl.edu> >> wrote: >> >>> Li, >>> >>> Mouse cardiac output seems to be about 17 ml/min (e.g. >>> www.transonic.com/mice1.shtml), you probably want to try for that to >>> keep pressures close to physiological. >>> A syringe pump is pretty inexpensive and probably all you need. >>> >>> Mike >>> >>> ----- Original Message ----- >>> From: Li Zhang < dancingwing@yahoo.com> >>> Date: Wednesday, March 17, 2010 14:59 >>> Subject: [Histonet] question about mouse perfusion >>> To: histonet@lists.utsouthwestern.edu >>> >>> > > My question is: can anyone give me a rough idea of how fast I >>> > > should inject ( like ml/min). I think I've tried like 30 ml in 3 >>> > > min, and I suspect that it's too fast because I do observe >>> > > tissue swelling sometimes. >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> Merced M Leiker >> Research Technician III >> Cardiovascular Medicine >> 348 Biomedical Research Building >> State University of New York at Buffalo >> 3435 Main St, Buffalo, NY 14214 USA >> leiker@buffalo.edu >> 716-829-6118 (Ph) >> 716-829-2665 (Fx) >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> ______________________________________________________________________ >> This email has been scanned by the MessageLabs Email Security System. >> For more information please visit http://www.messagelabs.com/email >> ______________________________________________________________________ > > > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Debra.Ortiz <@t> uchospitals.edu Mon Mar 29 13:26:28 2010 From: Debra.Ortiz <@t> uchospitals.edu (Debra.Ortiz@uchospitals.edu) Date: Mon Mar 29 13:26:43 2010 Subject: [Histonet] Coverglass Message-ID: <5392DB699B157E4C8E65B3779634BD7D01847A69@uchmbx04-hpk03s.UCHAD.uchospitals.edu> We are currently trying to find a manufacturer that may sell cover glass slips measuring 45 x 60. We found a box by Clay Adams, but can not find that size anymore in their catalog. Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** From mtitford <@t> aol.com Mon Mar 29 14:20:35 2010 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Mon Mar 29 14:20:59 2010 Subject: [Histonet] Is my Leica CM 1850 cryostat haunted? Message-ID: <8CC9D8C0AA7A02F-B40-5D8F@webmail-m081.sysops.aol.com> We have a Leica CM 1850 cryostat that about once every three weeks to a month, turnes itself off! Has anyone else experienced this? How do I fix it? Initially, we thought someone after hours was turning it off, maybe someone in housekeeping or a passing med tech. That was not the case. Later our Biomedical people replaced a capacitor or something in the bowels of the cryostat thinking that may be faulty and contribute to the problem. That did not help either. Still later, the cryostat was put on its own circuit (No help), and then after that, we tried an uninterruptible power source thinking minor power fluctuations were the cause (no help either). Anyone know how to fix this problem?. It is disconcerting to walk into the lab in the morning and the cryostat is at room temperature and the OR is going to have a busy day. Michael Titford Pathology USA Mobile AL From TJJ <@t> stowers.org Mon Mar 29 15:37:59 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Mar 29 15:38:06 2010 Subject: [Histonet] Re: Acetone Fixation Message-ID: Atoska Gentry asked: hello, if any of you have acetone fixation incorporated into you frozen section H& E staining protocol will you please advise me on adjustments necessary for routine staining protocol. Also, out of curiosity please enlighten me on the purpose for post sectioning acetone fixation on tissue samples initially fixed in 95% ETOH/ Acetic Acid? Your prompt replies will be greatly appreciated. ~ Atoska Several thoughts come to mind. Acetone fixation is usually used only for preserving antigenicity on fresh frozen tissue samples. For slides needing H&E I would use a formalin fixative prior to the H&E procedure for fresh frozen tissues. Acetone fixed H&E stained cryosections are uuuuuuugly. You should not be doing frozen sections on tissues initially fixed in alcohol. Alcohol is an anti-freeze and you will not get good freezing/sectioning of the samples. You can section unfixed samples and then fix after mounting on glass slides in the Alcohol/acetic acid fix. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From mwich <@t> 7thwavelabs.com Mon Mar 29 15:40:35 2010 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Mon Mar 29 15:40:41 2010 Subject: [Histonet] osteopontin Message-ID: <62A8156F8071C8439080D626DF8C33A6CEB48C@wave-mail.7thwave.local> Can anyone recommend an osteopontin antibody for inflammation that works in FFPE mouse tissue, in particular mouse paws? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From mshaeffer <@t> cox.net Mon Mar 29 17:06:57 2010 From: mshaeffer <@t> cox.net (Marc & Sandy Shaeffer) Date: Mon Mar 29 17:07:01 2010 Subject: [Histonet] Learn From the Best, Head West! Message-ID: <21840790.14821.1269900417148.JavaMail.mshaeffer@127.0.0.1> The Arizona Society for Histotechnology (ASH) is hosting the Region VII Summer Symposium June 4 - June 6 2010 at the Embassy Suites Phoenix North, 2577 W. Greenway Road, Phoenix, AZ 85023. Attendees with paid registration by May 15, 2010 will get a chance to win $100.00 cash. Guest speakers: Friday Jun 4 8 am - 11:30 am - Basic Chemistry and Lab Math for the HT, presented by Ada Feldman - LEAN Principles in Histology, presented by William DeSalvo - Immuno-staining of Cytological Materials: Application and Pitfalls, presented by Joseph Myers Friday Jun 4 1:00 pm - 4:30 pm - Rapid Processing: Microwave Style, presented by Donna Willis - It's your Health; It's your Safety, Julia Rosen and Dan Williams - This Doesn't Happen When I Stain Manually!, Peggy McArthur Saturday Jun 5 8 am - 11:30 am - Benefits of Multi-antigen Immuno-Staining, presented by Joseph Myers - HT/HTL Exam PART I, presented by Robert Lott - Knowing You, Knowing Me, presented by Beth Roche and Roger Strickland Saturday Jun 5 1:30 pm - 5:00 pm - Quality Control and Standardization of IHC, Kelsey Jones - HT/HTL Exam PART II, Robert Lott - Novel Detection Chemistry, Traci DeGeer Sunday Jun 6 8:00 am - 11:30 am - Tissue identification Made Easy, presented by Ada Feldman - Antibody Challenge 2010, Kelsey Jones and Ourhay Shamoon - Water Quality and Standards for the Histology Laboratory, Ethel Macrea Special events planned for Friday and Saturday evenings. For detailed brochure e-mail mshaeffer@cox.net See you there, Marc Shaeffer ASH Secretary From TJJ <@t> stowers.org Mon Mar 29 17:18:40 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Mar 29 17:18:49 2010 Subject: [Histonet] MO Society for Histotechnology 2010 Spring Symposium Message-ID: Hi all! The Missouri Society for Histotechnology cordially invites you to our 2010 Spring Symposium for continuing education and histopathology technique and earn up to 12 CEUs. This year's conference will be held in: Saint Louis, Missouri Thursday, May 20th to Saturday, May 22nd We will stay at the Sheraton Westport Lakeside Chalet which is located on Westport Plaza, a centrally located 42 acres development offering an unparalleled combination of amenities and services, where you may enjoy over 20 restaurant and entertainment venues. It is nestled in the heart of St Louis and surrounded by everything exciting that the city has to offer. Room reservations can be made by calling 1-800-822-3535. Hotel reservation deadline for symposium rate is April 29, 2010. Reservations received after this date will be subject to room & rate availability. To secure your symposium rate please make your reservations early and indicate that you are attending the MSH symposium. Online room registration and meeting information is available at www.starwoodmeeting.com/Book/mohistotechnology. Visit our webpage for more information and links to the Symposium at www.missouri-histo.org/index.cfm For registration and more information about the program and work shop descritions, download the "Brochure 2010" and "Workshop Descriptions MHT 2010" in our downloads page. Obtain a discounted price on the symposium registration fee by becoming an active member of the society. Download a Membership/Renewal Application, fill it up and send it with your dues and your registration for the symposium. For more information, contact Amanda Kelley, MSH President, at Amanda.Kelley@stlukes-stl.com or Sharon Walsh at userwalsh@sbcglobal.net Hope to see you there! From AnthonyH <@t> chw.edu.au Mon Mar 29 20:20:50 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Mar 29 20:21:16 2010 Subject: [Histonet] Removing tissues from OCT for molecular analysis In-Reply-To: <7A4D7141-2408-42D4-94A6-8E606C498142@cellsignal.com> Message-ID: Katie, Rinse the frozen OCT surrounded tissue in Hanks or similar cell culture fluid. Two to three times should be enough to remove the OCT Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Katie Crosby Sent: Monday, 29 March 2010 11:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing tissues from OCT for molecular analysis Hello, Can someone suggest a means of removing tissues from OCT for subsequent molecular analysis? I did not find anything in the archives describing this exactly. Thanks, Katie Katie Crosby Immunohistochemistry Cell Signaling Technology 3 Trask Lane Danvers, MA 01923 Phone: 978-867-2352 Fax: 978-867-2400 WEB Site: www.cellsignal.com Toll Free: 1-877-616-CELL (877-616-2355) 1-877-678-TECH (877-678-8324) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From annigyg <@t> gmail.com Tue Mar 30 03:56:18 2010 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Tue Mar 30 03:56:27 2010 Subject: [Histonet] Is my Leica CM 1850 cryostat haunted? In-Reply-To: <8CC9D8C0AA7A02F-B40-5D8F@webmail-m081.sysops.aol.com> References: <8CC9D8C0AA7A02F-B40-5D8F@webmail-m081.sysops.aol.com> Message-ID: check the manual - maybe someone (long time ago) set up an auto defrost? Abu Dhabi Annie On 29 March 2010 23:20, wrote: > > > We have a Leica CM 1850 cryostat that about once every three weeks to a > month, turnes itself off! Has anyone else experienced this? How do I fix it? > > Initially, we thought someone after hours was turning it off, maybe someone > in housekeeping or a passing med tech. That was not the case. > Later our Biomedical people replaced a capacitor or something in the bowels > of the cryostat thinking that may be faulty and contribute to the problem. > That did not help either. > Still later, the cryostat was put on its own circuit (No help), and then > after that, we tried an uninterruptible power source thinking minor power > fluctuations were the cause (no help either). > > Anyone know how to fix this problem?. It is disconcerting to walk into the > lab in the morning and the cryostat is at room temperature and the OR is > going to have a busy day. > > Michael Titford > Pathology USA > Mobile AL > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From mtighe <@t> trudeauinstitute.org Tue Mar 30 07:36:08 2010 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Tue Mar 30 07:36:27 2010 Subject: [Histonet] IHCWORLD orders Message-ID: <4BB1B7AA.26E4.00EE.0@trudeauinstitute.org> I have recently tried to order a product from ihcworld and had no luck at all. Has anyone had this problem or is there anyone out there that is familiar with this company/website. I think that selling products may be new to this website. Any suggestions? Thanks! Mike From alexandra.meinl <@t> gmail.com Tue Mar 30 08:25:00 2010 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Tue Mar 30 08:25:06 2010 Subject: [Histonet] IHCWORLD orders In-Reply-To: <4BB1B7AA.26E4.00EE.0@trudeauinstitute.org> References: <4BB1B7AA.26E4.00EE.0@trudeauinstitute.org> Message-ID: Same here. I tried to purchase the biopsy punches with plunger for making control tissue TMAs, but had no success and finally found another company. ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.meinl@trauma.lbg.ac.at From azdudley <@t> hotmail.com Tue Mar 30 08:34:37 2010 From: azdudley <@t> hotmail.com (anita dudley) Date: Tue Mar 30 08:36:08 2010 Subject: [Histonet] (no subject) Message-ID: just wondering, how many slides are people doing for her2 validation. cap says 25 to 100, I have got 25 but my pathologist thinks we should do more? thanks, anita _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3 From azdudley <@t> hotmail.com Tue Mar 30 09:19:18 2010 From: azdudley <@t> hotmail.com (anita dudley) Date: Tue Mar 30 09:19:23 2010 Subject: [Histonet] her2 validation Message-ID: sorry!!! I didn't put a subject in the previous post. wondering about how many slides people are using for her2 validation. thanks anita dudley providence hospital mobile alabama _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3 From beth <@t> plantbio.uga.edu Tue Mar 30 09:46:40 2010 From: beth <@t> plantbio.uga.edu (Beth Richardson) Date: Tue Mar 30 09:46:48 2010 Subject: [Histonet] reichert-jung 2800 cryostat Message-ID: <5679BF44-E911-4898-94C8-2A4C4A8D86C5@plantbio.uga.edu> Hi all, Does anyone have a Reichert-Jung 2800 cryostat gathering dust? We need a drive board for a 2800 so if you have one you can spare or would sell please let me know. Thanks! Beth > Beth, > The answer is what I expected it to be. The drive board for the 2800 > is no longer available new or refurbished. The next option is to try > to find one laying around somewhere and worse case scenario it is a > manual cryostat. I would not expect much luck with somebody having > one collecting dust on a shelf. Sorry I don't have better news. > > David > David A. Benjamin > DBMS > DB MicroService, Inc. > (P) 770.904.4848 (P) 1.888.904.4848 > (F) 770.904.4855 (F) 1.888.902.4855 > www.dbmicroservice.com > www.dbsms.biz ********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department Miller Plant Sciences Bldg 120 Carlton Street University of Georgia Athens, GA 30602-7271 http://www.plantbio.uga.edu/emlab/ Phone - (706) 542-1790 & FAX - (706) 542-1805 "Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) ******************************************************************* "And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung) *************************************************************************** The Friends of the Marine Institute - Join Today! www.friendsofugami.com From jmcgough <@t> clinlab.com Tue Mar 30 11:25:49 2010 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Tue Mar 30 11:25:58 2010 Subject: [Histonet] GMS Fungus control In-Reply-To: Message-ID: I wondering if anybody out there would be willing to trade GMS fungus controls for AFB controls. Please contact me if interested. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com From algranth <@t> email.arizona.edu Tue Mar 30 11:32:19 2010 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Tue Mar 30 11:32:26 2010 Subject: [Histonet] organomercurials Message-ID: <131734B5-C16E-453A-BB54-682E7DDAAB93@email.arizona.edu> Does anybody know what precautions need to be taken when performing a Mercury Orange Stain for protein thiols? A friend of mine in another lab was asked to do this stain. The stain powder does have mercury in it and the MSDS calls for face masks/eye protection and gloves. Has anybody done this stain? Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. From vavalos <@t> allergydermatology.com Tue Mar 30 13:15:38 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Tue Mar 30 13:15:43 2010 Subject: [Histonet] Coverslipping with SubX Message-ID: <000001cad035$00d2f5b0$0278e110$@com> Is anyone using SubX ? I am trying it out and am having some difficulty coverslipping. I am using the Subx glue as directed since Acrymount didn't seem to work as well. I still get a hazy film under the glass and am getting big air bubbles as well. I can eventually get them out but its just takes a while and you know how time is precious when you have a line of slides to stain. The process is not as smooth as before. I really would like this to work out for me and eliminate xylene. Any suggestions?? Vanessa Fax: 602-277-2134 From mtitford <@t> aol.com Tue Mar 30 13:44:26 2010 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Tue Mar 30 13:44:49 2010 Subject: [Histonet] Haunted cryostat/Thanks! Message-ID: <8CC9E5028ADFB36-1500-2BE5@webmail-m010.sysops.aol.com> Thank you everyone who responded to my problem with a Leica CM 1850 cryostat turning itself off. About 11 people responded with tips. Thank you!! The Histonet is great!! In answer to some enquirys: 1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts for an hour. Jackie O' Conner asks if it is set to go back "on" after the defrost. I have no idea. It defrosts well the rest of the time, and turns itself back on. Brian Cornett-Early recommends changing the start device on the compressor. 2) Someone else asked if power is getting to the cryostat - Yes, when it turns itself off, the on/off switch is on "off". All you have to do is flip the switch and it starts pumping and cooling. 3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on the side of the compressor. That is probably where we will start. Mari Ann works for Leica so it sounds like good advice. Thank you everyone. Since it only breaks down about once a month, it will take a long time to determine if the problem is fixed. Michael Titford Pathology USA Mobile AL USA From Timothy.Morken <@t> ucsfmedctr.org Tue Mar 30 13:52:17 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Tue Mar 30 13:52:28 2010 Subject: [Histonet] GMS on decal tissue Message-ID: <1AAF670737F193429070841C6B2ADD4C013B16C780@EXMBMCB15.ucsfmedicalcenter.org> Has anyone seen or heard of problems with Grocott Methenamine Silver staining for fungi on decal tissues? Specifically, background or false positives? I can't find anything in any books that gives any indications to not use decaled tissue. Thanks! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.morken@ucsfmedctr.org From Dorothy.L.Webb <@t> HealthPartners.Com Tue Mar 30 14:03:55 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Mar 30 14:04:04 2010 Subject: [Histonet] questions Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C5786C5846@HPEMX3.HealthPartners.int> We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From foreightl <@t> gmail.com Tue Mar 30 15:26:21 2010 From: foreightl <@t> gmail.com (Pat Laurie) Date: Tue Mar 30 15:26:26 2010 Subject: [Histonet] her2 validation In-Reply-To: References: Message-ID: We left it up to our pathologist. Initially we did the 25 slide validation, our pathologist wasn't 100% convinced that he could read them accurately, so we did another 25, plus all of the ones we sent out which already had FISH ran. It ended up being almost 70 cases, and our pathologist was properly "educated" on how he should score them. He felt that there was excessive pressure put on the pathologists due to having to score these slides, not just say negative or positive. I'll say though for the last year or so, with about 6 her2 cases ran a day, he has been remarkably accurate. Any that he scores 2+ and has sent out for FISH he usually indicates if he thinks that it will be positive or negative. He has yet to be wrong. Another Pathologist who I talked to who helped set up the guidelines said that there isn't a "1 method fits all" process. Good luck. On Tue, Mar 30, 2010 at 7:19 AM, anita dudley wrote: > > sorry!!! I didn't put a subject in the previous post. wondering about how > many slides people are using for her2 validation. thanks > > > > anita dudley > > providence hospital > > mobile alabama > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your inbox. > > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com From Jackie.O'Connor <@t> abbott.com Tue Mar 30 15:28:30 2010 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Mar 30 15:29:04 2010 Subject: [Histonet] FREE MONEY! In-Reply-To: References: <002201caccb5$a448c5e0$ecda51a0$@at> Message-ID: Now that I have your attention - - I would really like to hear from you if you are at all interested in a histology position at Abbott Laboratories in Northern Illinois. Even if you have applied previously. This is a great opportunity to work in a GLP research lab, a good deal of bench work, but developing IHC for toxicology purposes as well. We have a Biocare intelliPATH stainer - it's FUN! We have four full time technicians/technologists with a fifth open position. Abbott offers 3 weeks paid vacation, great benefits, and is a good company to work for. I've been here ten years, and I've never been anywhere 10 years. Please forward your resume to me, as well as go to www.Abbott.com to apply. Jackie O'Connor From becky.garrison <@t> jax.ufl.edu Tue Mar 30 15:49:42 2010 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Tue Mar 30 15:49:47 2010 Subject: [Histonet] questions In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43C5786C5846@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43C5786C5846@HPEMX3.HealthPartners.int> Message-ID: We receive the placentas fresh (they are refrigerated in L&D before transport to Pathology); add lots of formalin (use 163 oz containers) and let fix overnight. Early next morning, placenta is grossed and cassettes sit in formalin til end of day. This formalin is changed at least once so that bloody formalin is replaced with fresh. Placed on processor at end of second day after receipt. When we had L&D add formalin, there was never enough formalin added and the placentas sat unfixed at room temperature for long periods of time. This procedure works better for us. Yes, we can not meet the CAP guideline for 2 day TAT but do end up with a consistently better quality product for this tissue type. (Placentas make up a small portion of overall workload, so overall TAT is not affected). Prior to this procedure, placentas made up a disproportionate amount of reprocessed blocks. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, March 30, 2010 3:04 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Tue Mar 30 18:04:19 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Mar 30 18:04:25 2010 Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's Message-ID: <465571.85431.qm@web113812.mail.gq1.yahoo.com> Hi All of you in Histoland, ? I am working?with a facility that recently purchased a Thermo Excelsior Tissue Processor.? They have had processing problems with several of their specimens using non-xylene clearing agent.? These issues were with both bx's and larger tissues.? The program and the non-xylene clearing agent was recommended by the technical staff at Thermo.? ? The histology staff?have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared. ? Also, the histotech's were using reagent alcohol, histological grade.? This grade of alcohol is denatured with methyl alcohol.? The sales representative was in and informed us that this type of alcohol?has damaging effects on the?instrument.? The representative will be coming in to flush out the system and reprogram the instrument next Monday.? ? I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with.? We are going to use basically the same program for the Excelsior, that we use on the VIP. ? I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs.? It would be great to?compare the reagents, programs,?timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs.? Thank you in advance for your assistance. Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com From kimtournear <@t> yahoo.com Tue Mar 30 18:05:40 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Mar 30 18:05:44 2010 Subject: [Histonet] re: cyto prep tech Message-ID: <839809.46245.qm@web54204.mail.re2.yahoo.com> Thank you to everyone who responded to my question about cyto prep tech work, ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! From histowa13 <@t> hotmail.com Tue Mar 30 19:12:57 2010 From: histowa13 <@t> hotmail.com (Debbie Nannenga) Date: Tue Mar 30 19:13:02 2010 Subject: [Histonet] her-2 neu validation Message-ID: Hi Anita, We ran 25 amplified and 25 negative cases for our validation study. Good Luck. Debbie Nannenga, HTL(ASCP) QIHC InCyte Pathology Spokane, WA From AnthonyH <@t> chw.edu.au Tue Mar 30 19:21:58 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 30 19:22:08 2010 Subject: [Histonet] questions In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43C5786C5846@HPEMX3.HealthPartners.int> Message-ID: Dorothy, Apart from hoping the blocks of tissue are not too thick, we microwave the cassettes in 10%NBF in a Milestone Mega TT - 2 hours at 45oC. This ensures adequate fixation prior to processing on a Shandon Excelsior Tissue Processor. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, 31 March 2010 6:04 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From conniegrubaugh <@t> hotmail.com Tue Mar 30 21:58:21 2010 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Tue Mar 30 21:58:26 2010 Subject: [Histonet] RE: Leica Paraplast In-Reply-To: <96793.49248.qm@web81005.mail.mud.yahoo.com> References: <201003231700.o2NH0hVP014125@flph259.prodigy.net>, <96793.49248.qm@web81005.mail.mud.yahoo.com> Message-ID: Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference. Connie G. > Date: Tue, 23 Mar 2010 11:29:03 -0700 > From: ddreesen@sbcglobal.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Paraplast > > > Hi Kristen, > We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block. > > > From: histonet-request@lists.utsouthwestern.edu Message: 4 > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) > From: kristen arvidson > Subject: [Histonet] Leica Paraplast > To: histonet > Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Has anyone who uses paraplast (we use the basic one) noticed a change in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft?s powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850552/direct/01/ From greenjumpyone <@t> hotmail.com Wed Mar 31 03:52:42 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Wed Mar 31 03:52:50 2010 Subject: [Histonet] (no subject) Message-ID: http://www.trainedlabor.com/H6UH4Yh1UJ.html _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3 From malbenatti <@t> googlemail.com Wed Mar 31 04:45:09 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Wed Mar 31 04:45:20 2010 Subject: Fwd: [Histonet] (no subject) References: Message-ID: <8186A0A0-94D8-4E31-99D1-87B35FC3CDC6@gmail.com> Dear Histonet list manager Is they anyway you could filter/block spam such as this one from be sent out to the Histonet list. Cheers Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London " ... Smile it confuses people ..." Begin forwarded message: > From: Green JumpyOne > Date: 31 March 2010 09:52:42 GMT+01:00 > To: , , > > Subject: [Histonet] (no subject) > > http://www.trainedlabor.com/H6UH4Yh1UJ.html > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your > inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> CHW.edu Wed Mar 31 07:02:26 2010 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed Mar 31 07:02:32 2010 Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's In-Reply-To: <465571.85431.qm@web113812.mail.gq1.yahoo.com> References: <465571.85431.qm@web113812.mail.gq1.yahoo.com> Message-ID: <2842DC75AE43AA4B92954CFB31781BC105697F76@CHW-MSG-301.chw.edu> Pretty much any problems I have had with the Excelsior and I have been using one for 4 years now is with Pathologists loading it incorrectly. I use reagent alcohol in it all the time, with no problems. I prefer using xylene in my processor. Every single time I have switched and started using a non-xylene sub. I have had nothing but problems. If I use a non-xylene I save it for the stainer. Take it easy, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Tuesday, March 30, 2010 4:04 PM To: histo net Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's Hi All of you in Histoland, ? I am working?with a facility that recently purchased a Thermo Excelsior Tissue Processor.? They have had processing problems with several of their specimens using non-xylene clearing agent.? These issues were with both bx's and larger tissues.? The program and the non-xylene clearing agent was recommended by the technical staff at Thermo.? ? The histology staff?have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared. ? Also, the histotech's were using reagent alcohol, histological grade.? This grade of alcohol is denatured with methyl alcohol.? The sales representative was in and informed us that this type of alcohol?has damaging effects on the?instrument.? The representative will be coming in to flush out the system and reprogram the instrument next Monday.? ? I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with.? We are going to use basically the same program for the Excelsior, that we use on the VIP. ? I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs.? It would be great to?compare the reagents, programs,?timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs.? Thank you in advance for your assistance. Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Wed Mar 31 07:27:34 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Mar 31 07:28:00 2010 Subject: [Histonet] RE: Leica Paraplast In-Reply-To: References: <201003231700.o2NH0hVP014125@flph259.prodigy.net>, <96793.49248.qm@web81005.mail.mud.yahoo.com> Message-ID: There is about three brands of paraplast and they all cut differently. In one of my labs I use paraplast plus and it ribbons very well however the blocks have to be colder then if you are using TissuePrep from Fisher. Leica may have sent you one of the other types of paraplast. (paraplast, Paraplast Plus, Paraplast Xtra) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: Tuesday, March 30, 2010 9:58 PM To: ddreesen@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Leica Paraplast Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference. Connie G. > Date: Tue, 23 Mar 2010 11:29:03 -0700 > From: ddreesen@sbcglobal.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Paraplast > > > Hi Kristen, > We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block. > > > From: histonet-request@lists.utsouthwestern.edu > Message: 4 > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) > From: kristen arvidson > Subject: [Histonet] Leica Paraplast > To: histonet > Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Has anyone who uses paraplast (we use the basic one) noticed a change in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850552/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From ann.bennettann <@t> yahoo.com Wed Mar 31 07:35:49 2010 From: ann.bennettann <@t> yahoo.com (Ann Bennett) Date: Wed Mar 31 07:35:54 2010 Subject: [Histonet] Thermo Fisher Excelsior Tissue Processor Programs Message-ID: <106398.85301.qm@web114318.mail.gq1.yahoo.com> At one point we looked at getting an Excelsior and I spoke with our sales rep and he said all the reagents we use in our processor now are absolutely fine.? He mentioned nothing about not being able to use Reagent alcohols or xylene substitutes.? Hope this helps - have a happy histo day! ? ? From kdwyer3322 <@t> aol.com Wed Mar 31 07:42:06 2010 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Wed Mar 31 07:42:28 2010 Subject: [Histonet] Texas Society for Histotechnology April 23-25, 2010 Message-ID: <8CC9EE6B49D9799-17C0-9D76@webmail-m051.sysops.aol.com> Histonetters, It is not too late to join us for the 2010 TSH meeting in Houston Texas April 23-25, 2010. The fun starts Thursday April 22, 2010 with a Golf outing open to all Vendors and Attendees. Workshops begin Friday April 23, 2010 at 8:00am. There is still plenty of time to register and get a hotel room to enjoy 2 full days of workshops and symposiums. If you would like a program go to txsh.org or contact me via this e-mail. Thanks, TSH Convention Committee From cmb321 <@t> excite.com Wed Mar 31 09:13:25 2010 From: cmb321 <@t> excite.com (Catherine Breen) Date: Wed Mar 31 09:13:29 2010 Subject: [Histonet] cassette labels erased by processor Message-ID: <20100331101325.1573@web007.roc2.bluetie.com> I am looking for help solving a lab mystery. The cassettes in our lab are labeled with an SP Securline Marker II and then processed in a Sakura VIP processor. Two weeks ago the entire batch came out labeled much more lightly than usual, some to the point of where the label was completely effaced. Our current theory is that an acid cleanser (Citronox) or possibly another acid was accidentally introduced into the processor. Has any lab experienced this problem before, especially with Citronox? Thank you. ------------------------------------------------------------ Best Weight Loss Program - Click Here! Weight Loss Program http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= From JWeems <@t> sjha.org Wed Mar 31 09:28:42 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Mar 31 09:28:47 2010 Subject: [Histonet] RE: her2 validation In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16405E029D9@CHEXCMS10.one.ads.che.org> >From June-15, 2009 CAP checklist ANP.22997 Phase I N/A YES NO If the laboratory performs HER2 testing (HER2 protein over-expression by immunohistochemistry [IHC] or HER2 gene amplification by in situ hybridization [e.g. FISH, CISH*, SISH*, etc.]), has the laboratory documented appropriate validation for the assay(s)? NOTE: Initial test validation must be performed on a minimum of 25 cases (recommended 25-100). Validation may be performed by comparing the results of testing with a validated alternative method (i.e. IHC vs. FISH) either in the same laboratory or another laboratory, or with the same validated method performed in another laboratory; validation testing must be done using the same set of cases in both labs. If specimens are fixed in a medium other than 10% neutral buffered formalin, the validation study must show that results are concordant with results from formalin-fixed tissues. If significant changes are made in testing methods (e.g., antibody clone, antigen retrieval protocol or detection system, FISH probe or pretreatment protocol), revalidation is required. This checklist item applies to laboratories that perform the technical testing of specimens for HER2 amplification. Patient specimens should be fixed in the same manner as the specimens used for the validation study(ies). *CISH = chromogenic in-situ hybridization; SISH = silver-enhanced in-situ hybridization Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From MSHERWOOD <@t> PARTNERS.ORG Wed Mar 31 09:33:35 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Mar 31 09:33:43 2010 Subject: [Histonet] cassette labels erased by processor In-Reply-To: <20100331101325.1573@web007.roc2.bluetie.com> References: <20100331101325.1573@web007.roc2.bluetie.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2414A@PHSXMB30.partners.org> I had similar issues with all of the marking pens out there. We finally switched to Tissue-Tek Marking pencils #4160 and have not had a problem since. Plus they never "dry" out! Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Breen Sent: Wednesday, March 31, 2010 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labels erased by processor I am looking for help solving a lab mystery. The cassettes in our lab are labeled with an SP Securline Marker II and then processed in a Sakura VIP processor. Two weeks ago the entire batch came out labeled much more lightly than usual, some to the point of where the label was completely effaced. Our current theory is that an acid cleanser (Citronox) or possibly another acid was accidentally introduced into the processor. Has any lab experienced this problem before, especially with Citronox? Thank you. ------------------------------------------------------------ Best Weight Loss Program - Click Here! Weight Loss Program http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOA AYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From cmiller <@t> physlab.com Wed Mar 31 09:39:46 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Mar 31 09:39:53 2010 Subject: FW: [Histonet] cassette labels erased by processor Message-ID: YES! That is why I use pencil only. I had a processor full of blank/ unlabeled cassettes because the lot# of the pens was bad. There is no way of knowing if the ink is reagent proof until a disaster like you have occurs. You don't always know if the ink lot passed its QC with absolute certainty. Pencil is fail proof. I hope your day gets better, Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Breen Sent: Wednesday, March 31, 2010 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labels erased by processor I PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From malbenatti <@t> googlemail.com Wed Mar 31 09:40:44 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Wed Mar 31 09:40:43 2010 Subject: [Histonet] cassette labels erased by processor Message-ID: Hi there, I would suggest to use a pencil rather than a marker pen to label cassettes when processing cassette with the Sakura VIP , my lab had a number of the so called solvent proof pen on trial and have yet to find one that survive processing. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London " ... Smile it confuses people ..." On 31 Mar 2010, at 15:13, "Catherine Breen" wrote: > I am looking for help solving a lab mystery. The cassettes in our > lab are labeled with an SP Securline Marker II and then processed in > a Sakura VIP processor. Two weeks ago the entire batch came out > labeled much more lightly than usual, some to the point of where the > label was completely effaced. > Our current theory is that an acid cleanser (Citronox) or possibly > another acid was accidentally introduced into the processor. > Has any lab experienced this problem before, especially with Citronox? > > Thank you. > > ------------------------------------------------------------ > Best Weight Loss Program - Click Here! > Weight Loss Program > http://tagline.excite.com/c? > cp= > etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Samuel_Perry <@t> DFCI.HARVARD.EDU Wed Mar 31 09:48:46 2010 From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel) Date: Wed Mar 31 09:48:53 2010 Subject: [Histonet] LC3-II Autophagy antibodies for IHC ? Message-ID: Hi, Tried post this the other day but I didn't see it go up. Can you make sure it gets on the mailing list. Thanks! -Sam "Hi All, I am trying to do IHC staining on FFPE mouse and human tissue to look for autophagy. Can anyone a recommend a good antibody which recognizes human or mouse LC3? In particular, I would hope that it recognizes only the autophagy specific membrane-bound LC3-II form not total LC3. Does such an antibody exist? Thanks -Sam Perry Dana-Farber Cancer Institute Research Technician " The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From drmtech09 <@t> gmail.com Wed Mar 31 09:54:36 2010 From: drmtech09 <@t> gmail.com (Eric Baltazar) Date: Wed Mar 31 09:54:40 2010 Subject: [Histonet] Wanted IEC-CTD cryostat Message-ID: <70f2f5ef1003310754n299a7f97i854b56e54e6f4eb4@mail.gmail.com> Hello fellow Histonetters! I'm looking for cryostat machine model IEC-CTD. They're the old ice cream box type used for frozen section, in case you have this park on your storage areas and not using it, I'm very much willing to purchase it regardless of its condition. You can call me at 323-4789871 or return me an email @ drmtech09@gmail.comfor this inquiry update.Thank you and looking forward for a favorable reply soon! From MadaryJ <@t> MedImmune.com Wed Mar 31 10:29:42 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Mar 31 10:29:51 2010 Subject: [Histonet] lab markers KP In-Reply-To: References: Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A130176AC22@MD1EV002.medimmune.com> The only consistent lab pen I have used that will hold up under decal, processing with various alcohol, xylene, hemo de is the KP marker plus. I am sure there are several vendors, I use Mercedes. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 31, 2010 10:45 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 45 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Coverslipping with SubX (Vanessa Avalos) 2. Haunted cryostat/Thanks! (mtitford@aol.com) 3. GMS on decal tissue (Morken, Tim) 4. questions (Webb, Dorothy L) 5. Re: her2 validation (Pat Laurie) 6. FREE MONEY! (Jackie M O'Connor) 7. RE: questions (Garrison, Becky) 8. Thermo's Excelsior Tissue Processor Program's (Akemi Allison-Tacha) 9. re: cyto prep tech (Kim Tournear) 10. her-2 neu validation (Debbie Nannenga) 11. RE: questions (Tony Henwood) 12. RE: RE: Leica Paraplast (connie grubaugh) 13. (no subject) (Green JumpyOne) 14. Fwd: [Histonet] (no subject) (Malika Benatti) 15. RE: Thermo's Excelsior Tissue Processor Program's (Heckford, Karen - SMMC-SF) 16. RE: RE: Leica Paraplast (Nails, Felton) 17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett) 18. Texas Society for Histotechnology April 23-25, 2010 (kdwyer3322@aol.com) 19. cassette labels erased by processor (Catherine Breen) 20. RE: her2 validation (Weems, Joyce) 21. RE: cassette labels erased by processor (Sherwood, Margaret ) 22. FW: [Histonet] cassette labels erased by processor (Cheri Miller) 23. Re: cassette labels erased by processor (Malika Benatti) ---------------------------------------------------------------------- Message: 1 Date: Tue, 30 Mar 2010 11:15:38 -0700 From: "Vanessa Avalos" Subject: [Histonet] Coverslipping with SubX To: "'HISTONET LISTS'" Message-ID: <000001cad035$00d2f5b0$0278e110$@com> Content-Type: text/plain; charset="us-ascii" Is anyone using SubX ? I am trying it out and am having some difficulty coverslipping. I am using the Subx glue as directed since Acrymount didn't seem to work as well. I still get a hazy film under the glass and am getting big air bubbles as well. I can eventually get them out but its just takes a while and you know how time is precious when you have a line of slides to stain. The process is not as smooth as before. I really would like this to work out for me and eliminate xylene. Any suggestions?? Vanessa Fax: 602-277-2134 ------------------------------ Message: 2 Date: Tue, 30 Mar 2010 14:44:26 -0400 From: mtitford@aol.com Subject: [Histonet] Haunted cryostat/Thanks! To: Histonet@pathology.swmed.edu Message-ID: <8CC9E5028ADFB36-1500-2BE5@webmail-m010.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Thank you everyone who responded to my problem with a Leica CM 1850 cryostat turning itself off. About 11 people responded with tips. Thank you!! The Histonet is great!! In answer to some enquirys: 1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts for an hour. Jackie O' Conner asks if it is set to go back "on" after the defrost. I have no idea. It defrosts well the rest of the time, and turns itself back on. Brian Cornett-Early recommends changing the start device on the compressor. 2) Someone else asked if power is getting to the cryostat - Yes, when it turns itself off, the on/off switch is on "off". All you have to do is flip the switch and it starts pumping and cooling. 3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on the side of the compressor. That is probably where we will start. Mari Ann works for Leica so it sounds like good advice. Thank you everyone. Since it only breaks down about once a month, it will take a long time to determine if the problem is fixed. Michael Titford Pathology USA Mobile AL USA ------------------------------ Message: 3 Date: Tue, 30 Mar 2010 11:52:17 -0700 From: "Morken, Tim" Subject: [Histonet] GMS on decal tissue To: histonet Message-ID: <1AAF670737F193429070841C6B2ADD4C013B16C780@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Has anyone seen or heard of problems with Grocott Methenamine Silver staining for fungi on decal tissues? Specifically, background or false positives? I can't find anything in any books that gives any indications to not use decaled tissue. Thanks! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.morken@ucsfmedctr.org ------------------------------ Message: 4 Date: Tue, 30 Mar 2010 14:03:55 -0500 From: "Webb, Dorothy L" Subject: [Histonet] questions To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C5786C5846@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 5 Date: Tue, 30 Mar 2010 13:26:21 -0700 From: Pat Laurie Subject: Re: [Histonet] her2 validation To: anita dudley Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We left it up to our pathologist. Initially we did the 25 slide validation, our pathologist wasn't 100% convinced that he could read them accurately, so we did another 25, plus all of the ones we sent out which already had FISH ran. It ended up being almost 70 cases, and our pathologist was properly "educated" on how he should score them. He felt that there was excessive pressure put on the pathologists due to having to score these slides, not just say negative or positive. I'll say though for the last year or so, with about 6 her2 cases ran a day, he has been remarkably accurate. Any that he scores 2+ and has sent out for FISH he usually indicates if he thinks that it will be positive or negative. He has yet to be wrong. Another Pathologist who I talked to who helped set up the guidelines said that there isn't a "1 method fits all" process. Good luck. On Tue, Mar 30, 2010 at 7:19 AM, anita dudley wrote: > > sorry!!! I didn't put a subject in the previous post. wondering about how > many slides people are using for her2 validation. thanks > > > > anita dudley > > providence hospital > > mobile alabama > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your inbox. > > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com ------------------------------ Message: 6 Date: Tue, 30 Mar 2010 15:28:30 -0500 From: Jackie M O'Connor Subject: [Histonet] FREE MONEY! To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Now that I have your attention - - I would really like to hear from you if you are at all interested in a histology position at Abbott Laboratories in Northern Illinois. Even if you have applied previously. This is a great opportunity to work in a GLP research lab, a good deal of bench work, but developing IHC for toxicology purposes as well. We have a Biocare intelliPATH stainer - it's FUN! We have four full time technicians/technologists with a fifth open position. Abbott offers 3 weeks paid vacation, great benefits, and is a good company to work for. I've been here ten years, and I've never been anywhere 10 years. Please forward your resume to me, as well as go to www.Abbott.com to apply. Jackie O'Connor ------------------------------ Message: 7 Date: Tue, 30 Mar 2010 16:49:42 -0400 From: "Garrison, Becky" Subject: RE: [Histonet] questions To: "Webb, Dorothy L" , Message-ID: Content-Type: text/plain; charset="us-ascii" We receive the placentas fresh (they are refrigerated in L&D before transport to Pathology); add lots of formalin (use 163 oz containers) and let fix overnight. Early next morning, placenta is grossed and cassettes sit in formalin til end of day. This formalin is changed at least once so that bloody formalin is replaced with fresh. Placed on processor at end of second day after receipt. When we had L&D add formalin, there was never enough formalin added and the placentas sat unfixed at room temperature for long periods of time. This procedure works better for us. Yes, we can not meet the CAP guideline for 2 day TAT but do end up with a consistently better quality product for this tissue type. (Placentas make up a small portion of overall workload, so overall TAT is not affected). Prior to this procedure, placentas made up a disproportionate amount of reprocessed blocks. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, March 30, 2010 3:04 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 30 Mar 2010 16:04:19 -0700 (PDT) From: Akemi Allison-Tacha Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's To: histo net Message-ID: <465571.85431.qm@web113812.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All of you in Histoland, ? I am working?with a facility that recently purchased a Thermo Excelsior Tissue Processor.? They have had processing problems with several of their specimens using non-xylene clearing agent.? These issues were with both bx's and larger tissues.? The program and the non-xylene clearing agent was recommended by the technical staff at Thermo.? ? The histology staff?have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared. ? Also, the histotech's were using reagent alcohol, histological grade.? This grade of alcohol is denatured with methyl alcohol.? The sales representative was in and informed us that this type of alcohol?has damaging effects on the?instrument.? The representative will be coming in to flush out the system and reprogram the instrument next Monday.? ? I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with.? We are going to use basically the same program for the Excelsior, that we use on the VIP. ? I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs.? It would be great to?compare the reagents, programs,?timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs.? Thank you in advance for your assistance. Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ------------------------------ Message: 9 Date: Tue, 30 Mar 2010 16:05:40 -0700 (PDT) From: Kim Tournear Subject: [Histonet] re: cyto prep tech To: Histonet Message-ID: <839809.46245.qm@web54204.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Thank you to everyone who responded to my question about cyto prep tech work, ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! ------------------------------ Message: 10 Date: Tue, 30 Mar 2010 17:12:57 -0700 From: Debbie Nannenga Subject: [Histonet] her-2 neu validation To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Anita, We ran 25 amplified and 25 negative cases for our validation study. Good Luck. Debbie Nannenga, HTL(ASCP) QIHC InCyte Pathology Spokane, WA ------------------------------ Message: 11 Date: Wed, 31 Mar 2010 11:21:58 +1100 From: "Tony Henwood" Subject: RE: [Histonet] questions To: "Webb, Dorothy L" , Message-ID: Content-Type: text/plain; charset="us-ascii" Dorothy, Apart from hoping the blocks of tissue are not too thick, we microwave the cassettes in 10%NBF in a Milestone Mega TT - 2 hours at 45oC. This ensures adequate fixation prior to processing on a Shandon Excelsior Tissue Processor. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, 31 March 2010 6:04 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 12 Date: Tue, 30 Mar 2010 19:58:21 -0700 From: connie grubaugh Subject: RE: [Histonet] RE: Leica Paraplast To: , Message-ID: Content-Type: text/plain; charset="Windows-1252" Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference. Connie G. > Date: Tue, 23 Mar 2010 11:29:03 -0700 > From: ddreesen@sbcglobal.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Paraplast > > > Hi Kristen, > We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block. > > > From: histonet-request@lists.utsouthwestern.edu Message: 4 > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) > From: kristen arvidson > Subject: [Histonet] Leica Paraplast > To: histonet > Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Has anyone who uses paraplast (we use the basic one) noticed a change in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850552/direct/01/ ------------------------------ Message: 13 Date: Wed, 31 Mar 2010 01:52:42 -0700 From: Green JumpyOne Subject: [Histonet] (no subject) To: , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" http://www.trainedlabor.com/H6UH4Yh1UJ.html _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3 ------------------------------ Message: 14 Date: Wed, 31 Mar 2010 10:45:09 +0100 From: Malika Benatti Subject: Fwd: [Histonet] (no subject) To: Histonet List Message-ID: <8186A0A0-94D8-4E31-99D1-87B35FC3CDC6@gmail.com> Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes Dear Histonet list manager Is they anyway you could filter/block spam such as this one from be sent out to the Histonet list. Cheers Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London " ... Smile it confuses people ..." Begin forwarded message: > From: Green JumpyOne > Date: 31 March 2010 09:52:42 GMT+01:00 > To: , , > > Subject: [Histonet] (no subject) > > http://www.trainedlabor.com/H6UH4Yh1UJ.html > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your > inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 31 Mar 2010 05:02:26 -0700 From: "Heckford, Karen - SMMC-SF" Subject: RE: [Histonet] Thermo's Excelsior Tissue Processor Program's To: "Akemi Allison-Tacha" Cc: histonet@lists.utsouthwestern.edu Message-ID: <2842DC75AE43AA4B92954CFB31781BC105697F76@CHW-MSG-301.chw.edu> Content-Type: text/plain; charset="iso-8859-1" Pretty much any problems I have had with the Excelsior and I have been using one for 4 years now is with Pathologists loading it incorrectly. I use reagent alcohol in it all the time, with no problems. I prefer using xylene in my processor. Every single time I have switched and started using a non-xylene sub. I have had nothing but problems. If I use a non-xylene I save it for the stainer. Take it easy, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Tuesday, March 30, 2010 4:04 PM To: histo net Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's Hi All of you in Histoland, ? I am working?with a facility that recently purchased a Thermo Excelsior Tissue Processor.? They have had processing problems with several of their specimens using non-xylene clearing agent.? These issues were with both bx's and larger tissues.? The program and the non-xylene clearing agent was recommended by the technical staff at Thermo.? ? The histology staff?have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared. ? Also, the histotech's were using reagent alcohol, histological grade.? This grade of alcohol is denatured with methyl alcohol.? The sales representative was in and informed us that this type of alcohol?has damaging effects on the?instrument.? The representative will be coming in to flush out the system and reprogram the instrument next Monday.? ? I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with.? We are going to use basically the same program for the Excelsior, that we use on the VIP. ? I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs.? It would be great to?compare the reagents, programs,?timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs.? Thank you in advance for your assistance. Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 31 Mar 2010 07:27:34 -0500 From: "Nails, Felton" Subject: RE: [Histonet] RE: Leica Paraplast To: "'connie grubaugh'" , "ddreesen@sbcglobal.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii There is about three brands of paraplast and they all cut differently. In one of my labs I use paraplast plus and it ribbons very well however the blocks have to be colder then if you are using TissuePrep from Fisher. Leica may have sent you one of the other types of paraplast. (paraplast, Paraplast Plus, Paraplast Xtra) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: Tuesday, March 30, 2010 9:58 PM To: ddreesen@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Leica Paraplast Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference. Connie G. > Date: Tue, 23 Mar 2010 11:29:03 -0700 > From: ddreesen@sbcglobal.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Paraplast > > > Hi Kristen, > We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block. > > > From: histonet-request@lists.utsouthwestern.edu > Message: 4 > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) > From: kristen arvidson > Subject: [Histonet] Leica Paraplast > To: histonet > Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Has anyone who uses paraplast (we use the basic one) noticed a change in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850552/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ ------------------------------ Message: 17 Date: Wed, 31 Mar 2010 05:35:49 -0700 (PDT) From: Ann Bennett Subject: [Histonet] Thermo Fisher Excelsior Tissue Processor Programs To: histonet@lists.utsouthwestern.edu Message-ID: <106398.85301.qm@web114318.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 At one point we looked at getting an Excelsior and I spoke with our sales rep and he said all the reagents we use in our processor now are absolutely fine.? He mentioned nothing about not being able to use Reagent alcohols or xylene substitutes.? Hope this helps - have a happy histo day! ? ? ------------------------------ Message: 18 Date: Wed, 31 Mar 2010 08:42:06 -0400 From: kdwyer3322@aol.com Subject: [Histonet] Texas Society for Histotechnology April 23-25, 2010 To: histonet@lists.utsouthwestern.edu Message-ID: <8CC9EE6B49D9799-17C0-9D76@webmail-m051.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Histonetters, It is not too late to join us for the 2010 TSH meeting in Houston Texas April 23-25, 2010. The fun starts Thursday April 22, 2010 with a Golf outing open to all Vendors and Attendees. Workshops begin Friday April 23, 2010 at 8:00am. There is still plenty of time to register and get a hotel room to enjoy 2 full days of workshops and symposiums. If you would like a program go to txsh.org or contact me via this e-mail. Thanks, TSH Convention Committee ------------------------------ Message: 19 Date: Wed, 31 Mar 2010 10:13:25 -0400 From: "Catherine Breen" Subject: [Histonet] cassette labels erased by processor To: histonet@lists.utsouthwestern.edu Message-ID: <20100331101325.1573@web007.roc2.bluetie.com> Content-Type: text/plain; charset=UTF-8 I am looking for help solving a lab mystery. The cassettes in our lab are labeled with an SP Securline Marker II and then processed in a Sakura VIP processor. Two weeks ago the entire batch came out labeled much more lightly than usual, some to the point of where the label was completely effaced. Our current theory is that an acid cleanser (Citronox) or possibly another acid was accidentally introduced into the processor. Has any lab experienced this problem before, especially with Citronox? Thank you. ------------------------------------------------------------ Best Weight Loss Program - Click Here! Weight Loss Program http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= ------------------------------ Message: 20 Date: Wed, 31 Mar 2010 10:28:42 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: her2 validation To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16405E029D9@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" >From June-15, 2009 CAP checklist ANP.22997 Phase I N/A YES NO If the laboratory performs HER2 testing (HER2 protein over-expression by immunohistochemistry [IHC] or HER2 gene amplification by in situ hybridization [e.g. FISH, CISH*, SISH*, etc.]), has the laboratory documented appropriate validation for the assay(s)? NOTE: Initial test validation must be performed on a minimum of 25 cases (recommended 25-100). Validation may be performed by comparing the results of testing with a validated alternative method (i.e. IHC vs. FISH) either in the same laboratory or another laboratory, or with the same validated method performed in another laboratory; validation testing must be done using the same set of cases in both labs. If specimens are fixed in a medium other than 10% neutral buffered formalin, the validation study must show that results are concordant with results from formalin-fixed tissues. If significant changes are made in testing methods (e.g., antibody clone, antigen retrieval protocol or detection system, FISH probe or pretreatment protocol), revalidation is required. This checklist item applies to laboratories that perform the technical testing of specimens for HER2 amplification. Patient specimens should be fixed in the same manner as the specimens used for the validation study(ies). *CISH = chromogenic in-situ hybridization; SISH = silver-enhanced in-situ hybridization Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 21 Date: Wed, 31 Mar 2010 10:33:35 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] cassette labels erased by processor To: "Catherine Breen" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2414A@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" I had similar issues with all of the marking pens out there. We finally switched to Tissue-Tek Marking pencils #4160 and have not had a problem since. Plus they never "dry" out! Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Breen Sent: Wednesday, March 31, 2010 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labels erased by processor I am looking for help solving a lab mystery. The cassettes in our lab are labeled with an SP Securline Marker II and then processed in a Sakura VIP processor. Two weeks ago the entire batch came out labeled much more lightly than usual, some to the point of where the label was completely effaced. Our current theory is that an acid cleanser (Citronox) or possibly another acid was accidentally introduced into the processor. Has any lab experienced this problem before, especially with Citronox? Thank you. ------------------------------------------------------------ Best Weight Loss Program - Click Here! Weight Loss Program http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOA AYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 22 Date: Wed, 31 Mar 2010 09:39:46 -0500 From: Cheri Miller Subject: FW: [Histonet] cassette labels erased by processor To: "histonet-bounces@lists.utsouthwestern.edu" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" YES! That is why I use pencil only. I had a processor full of blank/ unlabeled cassettes because the lot# of the pens was bad. There is no way of knowing if the ink is reagent proof until a disaster like you have occurs. You don't always know if the ink lot passed its QC with absolute certainty. Pencil is fail proof. I hope your day gets better, Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Breen Sent: Wednesday, March 31, 2010 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labels erased by processor I PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 23 Date: Wed, 31 Mar 2010 15:40:44 +0100 From: Malika Benatti Subject: Re: [Histonet] cassette labels erased by processor To: Catherine Breen Cc: Histonet List Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes Hi there, I would suggest to use a pencil rather than a marker pen to label cassettes when processing cassette with the Sakura VIP , my lab had a number of the so called solvent proof pen on trial and have yet to find one that survive processing. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London " ... Smile it confuses people ..." On 31 Mar 2010, at 15:13, "Catherine Breen" wrote: > I am looking for help solving a lab mystery. The cassettes in our > lab are labeled with an SP Securline Marker II and then processed in > a Sakura VIP processor. Two weeks ago the entire batch came out > labeled much more lightly than usual, some to the point of where the > label was completely effaced. > Our current theory is that an acid cleanser (Citronox) or possibly > another acid was accidentally introduced into the processor. > Has any lab experienced this problem before, especially with Citronox? > > Thank you. > > ------------------------------------------------------------ > Best Weight Loss Program - Click Here! > Weight Loss Program > http://tagline.excite.com/c? > cp= > etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 45 **************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From lblazek <@t> digestivespecialists.com Wed Mar 31 10:31:28 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Mar 31 10:31:36 2010 Subject: [Histonet] RE: lab markers KP In-Reply-To: <29A3CB81288E6F4BA2C9B3C8015A9A130176AC22@MD1EV002.medimmune.com> References: <29A3CB81288E6F4BA2C9B3C8015A9A130176AC22@MD1EV002.medimmune.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390E9BF7681D@IBMB7Exchange.digestivespecialists.com> I have to agree. They have never failed and work forever. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Wednesday, March 31, 2010 11:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab markers KP The only consistent lab pen I have used that will hold up under decal, processing with various alcohol, xylene, hemo de is the KP marker plus. I am sure there are several vendors, I use Mercedes. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 31, 2010 10:45 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 45 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Coverslipping with SubX (Vanessa Avalos) 2. Haunted cryostat/Thanks! (mtitford@aol.com) 3. GMS on decal tissue (Morken, Tim) 4. questions (Webb, Dorothy L) 5. Re: her2 validation (Pat Laurie) 6. FREE MONEY! (Jackie M O'Connor) 7. RE: questions (Garrison, Becky) 8. Thermo's Excelsior Tissue Processor Program's (Akemi Allison-Tacha) 9. re: cyto prep tech (Kim Tournear) 10. her-2 neu validation (Debbie Nannenga) 11. RE: questions (Tony Henwood) 12. RE: RE: Leica Paraplast (connie grubaugh) 13. (no subject) (Green JumpyOne) 14. Fwd: [Histonet] (no subject) (Malika Benatti) 15. RE: Thermo's Excelsior Tissue Processor Program's (Heckford, Karen - SMMC-SF) 16. RE: RE: Leica Paraplast (Nails, Felton) 17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett) 18. Texas Society for Histotechnology April 23-25, 2010 (kdwyer3322@aol.com) 19. cassette labels erased by processor (Catherine Breen) 20. RE: her2 validation (Weems, Joyce) 21. RE: cassette labels erased by processor (Sherwood, Margaret ) 22. FW: [Histonet] cassette labels erased by processor (Cheri Miller) 23. Re: cassette labels erased by processor (Malika Benatti) ---------------------------------------------------------------------- Message: 1 Date: Tue, 30 Mar 2010 11:15:38 -0700 From: "Vanessa Avalos" Subject: [Histonet] Coverslipping with SubX To: "'HISTONET LISTS'" Message-ID: <000001cad035$00d2f5b0$0278e110$@com> Content-Type: text/plain; charset="us-ascii" Is anyone using SubX ? I am trying it out and am having some difficulty coverslipping. I am using the Subx glue as directed since Acrymount didn't seem to work as well. I still get a hazy film under the glass and am getting big air bubbles as well. I can eventually get them out but its just takes a while and you know how time is precious when you have a line of slides to stain. The process is not as smooth as before. I really would like this to work out for me and eliminate xylene. Any suggestions?? Vanessa Fax: 602-277-2134 ------------------------------ Message: 2 Date: Tue, 30 Mar 2010 14:44:26 -0400 From: mtitford@aol.com Subject: [Histonet] Haunted cryostat/Thanks! To: Histonet@pathology.swmed.edu Message-ID: <8CC9E5028ADFB36-1500-2BE5@webmail-m010.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Thank you everyone who responded to my problem with a Leica CM 1850 cryostat turning itself off. About 11 people responded with tips. Thank you!! The Histonet is great!! In answer to some enquirys: 1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts for an hour. Jackie O' Conner asks if it is set to go back "on" after the defrost. I have no idea. It defrosts well the rest of the time, and turns itself back on. Brian Cornett-Early recommends changing the start device on the compressor. 2) Someone else asked if power is getting to the cryostat - Yes, when it turns itself off, the on/off switch is on "off". All you have to do is flip the switch and it starts pumping and cooling. 3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on the side of the compressor. That is probably where we will start. Mari Ann works for Leica so it sounds like good advice. Thank you everyone. Since it only breaks down about once a month, it will take a long time to determine if the problem is fixed. Michael Titford Pathology USA Mobile AL USA ------------------------------ Message: 3 Date: Tue, 30 Mar 2010 11:52:17 -0700 From: "Morken, Tim" Subject: [Histonet] GMS on decal tissue To: histonet Message-ID: <1AAF670737F193429070841C6B2ADD4C013B16C780@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Has anyone seen or heard of problems with Grocott Methenamine Silver staining for fungi on decal tissues? Specifically, background or false positives? I can't find anything in any books that gives any indications to not use decaled tissue. Thanks! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.morken@ucsfmedctr.org ------------------------------ Message: 4 Date: Tue, 30 Mar 2010 14:03:55 -0500 From: "Webb, Dorothy L" Subject: [Histonet] questions To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C5786C5846@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 5 Date: Tue, 30 Mar 2010 13:26:21 -0700 From: Pat Laurie Subject: Re: [Histonet] her2 validation To: anita dudley Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We left it up to our pathologist. Initially we did the 25 slide validation, our pathologist wasn't 100% convinced that he could read them accurately, so we did another 25, plus all of the ones we sent out which already had FISH ran. It ended up being almost 70 cases, and our pathologist was properly "educated" on how he should score them. He felt that there was excessive pressure put on the pathologists due to having to score these slides, not just say negative or positive. I'll say though for the last year or so, with about 6 her2 cases ran a day, he has been remarkably accurate. Any that he scores 2+ and has sent out for FISH he usually indicates if he thinks that it will be positive or negative. He has yet to be wrong. Another Pathologist who I talked to who helped set up the guidelines said that there isn't a "1 method fits all" process. Good luck. On Tue, Mar 30, 2010 at 7:19 AM, anita dudley wrote: > > sorry!!! I didn't put a subject in the previous post. wondering about how > many slides people are using for her2 validation. thanks > > > > anita dudley > > providence hospital > > mobile alabama > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your inbox. > > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com ------------------------------ Message: 6 Date: Tue, 30 Mar 2010 15:28:30 -0500 From: Jackie M O'Connor Subject: [Histonet] FREE MONEY! To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Now that I have your attention - - I would really like to hear from you if you are at all interested in a histology position at Abbott Laboratories in Northern Illinois. Even if you have applied previously. This is a great opportunity to work in a GLP research lab, a good deal of bench work, but developing IHC for toxicology purposes as well. We have a Biocare intelliPATH stainer - it's FUN! We have four full time technicians/technologists with a fifth open position. Abbott offers 3 weeks paid vacation, great benefits, and is a good company to work for. I've been here ten years, and I've never been anywhere 10 years. Please forward your resume to me, as well as go to www.Abbott.com to apply. Jackie O'Connor ------------------------------ Message: 7 Date: Tue, 30 Mar 2010 16:49:42 -0400 From: "Garrison, Becky" Subject: RE: [Histonet] questions To: "Webb, Dorothy L" , Message-ID: Content-Type: text/plain; charset="us-ascii" We receive the placentas fresh (they are refrigerated in L&D before transport to Pathology); add lots of formalin (use 163 oz containers) and let fix overnight. Early next morning, placenta is grossed and cassettes sit in formalin til end of day. This formalin is changed at least once so that bloody formalin is replaced with fresh. Placed on processor at end of second day after receipt. When we had L&D add formalin, there was never enough formalin added and the placentas sat unfixed at room temperature for long periods of time. This procedure works better for us. Yes, we can not meet the CAP guideline for 2 day TAT but do end up with a consistently better quality product for this tissue type. (Placentas make up a small portion of overall workload, so overall TAT is not affected). Prior to this procedure, placentas made up a disproportionate amount of reprocessed blocks. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, March 30, 2010 3:04 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 30 Mar 2010 16:04:19 -0700 (PDT) From: Akemi Allison-Tacha Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's To: histo net Message-ID: <465571.85431.qm@web113812.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All of you in Histoland, I am working with a facility that recently purchased a Thermo Excelsior Tissue Processor. They have had processing problems with several of their specimens using non-xylene clearing agent. These issues were with both bx's and larger tissues. The program and the non-xylene clearing agent was recommended by the technical staff at Thermo. The histology staff have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared. Also, the histotech's were using reagent alcohol, histological grade. This grade of alcohol is denatured with methyl alcohol. The sales representative was in and informed us that this type of alcohol has damaging effects on the instrument. The representative will be coming in to flush out the system and reprogram the instrument next Monday. I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with. We are going to use basically the same program for the Excelsior, that we use on the VIP. I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs. It would be great to compare the reagents, programs, timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs. Thank you in advance for your assistance. Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ------------------------------ Message: 9 Date: Tue, 30 Mar 2010 16:05:40 -0700 (PDT) From: Kim Tournear Subject: [Histonet] re: cyto prep tech To: Histonet Message-ID: <839809.46245.qm@web54204.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Thank you to everyone who responded to my question about cyto prep tech work, ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! ------------------------------ Message: 10 Date: Tue, 30 Mar 2010 17:12:57 -0700 From: Debbie Nannenga Subject: [Histonet] her-2 neu validation To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Anita, We ran 25 amplified and 25 negative cases for our validation study. Good Luck. Debbie Nannenga, HTL(ASCP) QIHC InCyte Pathology Spokane, WA ------------------------------ Message: 11 Date: Wed, 31 Mar 2010 11:21:58 +1100 From: "Tony Henwood" Subject: RE: [Histonet] questions To: "Webb, Dorothy L" , Message-ID: Content-Type: text/plain; charset="us-ascii" Dorothy, Apart from hoping the blocks of tissue are not too thick, we microwave the cassettes in 10%NBF in a Milestone Mega TT - 2 hours at 45oC. This ensures adequate fixation prior to processing on a Shandon Excelsior Tissue Processor. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, 31 March 2010 6:04 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 12 Date: Tue, 30 Mar 2010 19:58:21 -0700 From: connie grubaugh Subject: RE: [Histonet] RE: Leica Paraplast To: , Message-ID: Content-Type: text/plain; charset="Windows-1252" Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference. Connie G. > Date: Tue, 23 Mar 2010 11:29:03 -0700 > From: ddreesen@sbcglobal.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Paraplast > > > Hi Kristen, > We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block. > > > From: histonet-request@lists.utsouthwestern.edu Message: 4 > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) > From: kristen arvidson > Subject: [Histonet] Leica Paraplast > To: histonet > Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Has anyone who uses paraplast (we use the basic one) noticed a change in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850552/direct/01/ ------------------------------ Message: 13 Date: Wed, 31 Mar 2010 01:52:42 -0700 From: Green JumpyOne Subject: [Histonet] (no subject) To: , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" http://www.trainedlabor.com/H6UH4Yh1UJ.html _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3 ------------------------------ Message: 14 Date: Wed, 31 Mar 2010 10:45:09 +0100 From: Malika Benatti Subject: Fwd: [Histonet] (no subject) To: Histonet List Message-ID: <8186A0A0-94D8-4E31-99D1-87B35FC3CDC6@gmail.com> Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes Dear Histonet list manager Is they anyway you could filter/block spam such as this one from be sent out to the Histonet list. Cheers Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London " ... Smile it confuses people ..." Begin forwarded message: > From: Green JumpyOne > Date: 31 March 2010 09:52:42 GMT+01:00 > To: , , > > Subject: [Histonet] (no subject) > > http://www.trainedlabor.com/H6UH4Yh1UJ.html > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your > inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 31 Mar 2010 05:02:26 -0700 From: "Heckford, Karen - SMMC-SF" Subject: RE: [Histonet] Thermo's Excelsior Tissue Processor Program's To: "Akemi Allison-Tacha" Cc: histonet@lists.utsouthwestern.edu Message-ID: <2842DC75AE43AA4B92954CFB31781BC105697F76@CHW-MSG-301.chw.edu> Content-Type: text/plain; charset="iso-8859-1" Pretty much any problems I have had with the Excelsior and I have been using one for 4 years now is with Pathologists loading it incorrectly. I use reagent alcohol in it all the time, with no problems. I prefer using xylene in my processor. Every single time I have switched and started using a non-xylene sub. I have had nothing but problems. If I use a non-xylene I save it for the stainer. Take it easy, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Tuesday, March 30, 2010 4:04 PM To: histo net Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's Hi All of you in Histoland, I am working with a facility that recently purchased a Thermo Excelsior Tissue Processor. They have had processing problems with several of their specimens using non-xylene clearing agent. These issues were with both bx's and larger tissues. The program and the non-xylene clearing agent was recommended by the technical staff at Thermo. The histology staff have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared. Also, the histotech's were using reagent alcohol, histological grade. This grade of alcohol is denatured with methyl alcohol. The sales representative was in and informed us that this type of alcohol has damaging effects on the instrument. The representative will be coming in to flush out the system and reprogram the instrument next Monday. I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with. We are going to use basically the same program for the Excelsior, that we use on the VIP. I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs. It would be great to compare the reagents, programs, timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs. Thank you in advance for your assistance. Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 31 Mar 2010 07:27:34 -0500 From: "Nails, Felton" Subject: RE: [Histonet] RE: Leica Paraplast To: "'connie grubaugh'" , "ddreesen@sbcglobal.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii There is about three brands of paraplast and they all cut differently. In one of my labs I use paraplast plus and it ribbons very well however the blocks have to be colder then if you are using TissuePrep from Fisher. Leica may have sent you one of the other types of paraplast. (paraplast, Paraplast Plus, Paraplast Xtra) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: Tuesday, March 30, 2010 9:58 PM To: ddreesen@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Leica Paraplast Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference. Connie G. > Date: Tue, 23 Mar 2010 11:29:03 -0700 > From: ddreesen@sbcglobal.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Paraplast > > > Hi Kristen, > We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block. > > > From: histonet-request@lists.utsouthwestern.edu > Message: 4 > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) > From: kristen arvidson > Subject: [Histonet] Leica Paraplast > To: histonet > Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Has anyone who uses paraplast (we use the basic one) noticed a change in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850552/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ ------------------------------ Message: 17 Date: Wed, 31 Mar 2010 05:35:49 -0700 (PDT) From: Ann Bennett Subject: [Histonet] Thermo Fisher Excelsior Tissue Processor Programs To: histonet@lists.utsouthwestern.edu Message-ID: <106398.85301.qm@web114318.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 At one point we looked at getting an Excelsior and I spoke with our sales rep and he said all the reagents we use in our processor now are absolutely fine. He mentioned nothing about not being able to use Reagent alcohols or xylene substitutes. Hope this helps - have a happy histo day! ------------------------------ Message: 18 Date: Wed, 31 Mar 2010 08:42:06 -0400 From: kdwyer3322@aol.com Subject: [Histonet] Texas Society for Histotechnology April 23-25, 2010 To: histonet@lists.utsouthwestern.edu Message-ID: <8CC9EE6B49D9799-17C0-9D76@webmail-m051.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Histonetters, It is not too late to join us for the 2010 TSH meeting in Houston Texas April 23-25, 2010. The fun starts Thursday April 22, 2010 with a Golf outing open to all Vendors and Attendees. Workshops begin Friday April 23, 2010 at 8:00am. There is still plenty of time to register and get a hotel room to enjoy 2 full days of workshops and symposiums. If you would like a program go to txsh.org or contact me via this e-mail. Thanks, TSH Convention Committee ------------------------------ Message: 19 Date: Wed, 31 Mar 2010 10:13:25 -0400 From: "Catherine Breen" Subject: [Histonet] cassette labels erased by processor To: histonet@lists.utsouthwestern.edu Message-ID: <20100331101325.1573@web007.roc2.bluetie.com> Content-Type: text/plain; charset=UTF-8 I am looking for help solving a lab mystery. The cassettes in our lab are labeled with an SP Securline Marker II and then processed in a Sakura VIP processor. Two weeks ago the entire batch came out labeled much more lightly than usual, some to the point of where the label was completely effaced. Our current theory is that an acid cleanser (Citronox) or possibly another acid was accidentally introduced into the processor. Has any lab experienced this problem before, especially with Citronox? Thank you. ------------------------------------------------------------ Best Weight Loss Program - Click Here! Weight Loss Program http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= ------------------------------ Message: 20 Date: Wed, 31 Mar 2010 10:28:42 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: her2 validation To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16405E029D9@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" >From June-15, 2009 CAP checklist ANP.22997 Phase I N/A YES NO If the laboratory performs HER2 testing (HER2 protein over-expression by immunohistochemistry [IHC] or HER2 gene amplification by in situ hybridization [e.g. FISH, CISH*, SISH*, etc.]), has the laboratory documented appropriate validation for the assay(s)? NOTE: Initial test validation must be performed on a minimum of 25 cases (recommended 25-100). Validation may be performed by comparing the results of testing with a validated alternative method (i.e. IHC vs. FISH) either in the same laboratory or another laboratory, or with the same validated method performed in another laboratory; validation testing must be done using the same set of cases in both labs. If specimens are fixed in a medium other than 10% neutral buffered formalin, the validation study must show that results are concordant with results from formalin-fixed tissues. If significant changes are made in testing methods (e.g., antibody clone, antigen retrieval protocol or detection system, FISH probe or pretreatment protocol), revalidation is required. This checklist item applies to laboratories that perform the technical testing of specimens for HER2 amplification. Patient specimens should be fixed in the same manner as the specimens used for the validation study(ies). *CISH = chromogenic in-situ hybridization; SISH = silver-enhanced in-situ hybridization Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 21 Date: Wed, 31 Mar 2010 10:33:35 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] cassette labels erased by processor To: "Catherine Breen" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2414A@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" I had similar issues with all of the marking pens out there. We finally switched to Tissue-Tek Marking pencils #4160 and have not had a problem since. Plus they never "dry" out! Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Breen Sent: Wednesday, March 31, 2010 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labels erased by processor I am looking for help solving a lab mystery. The cassettes in our lab are labeled with an SP Securline Marker II and then processed in a Sakura VIP processor. Two weeks ago the entire batch came out labeled much more lightly than usual, some to the point of where the label was completely effaced. Our current theory is that an acid cleanser (Citronox) or possibly another acid was accidentally introduced into the processor. Has any lab experienced this problem before, especially with Citronox? Thank you. ------------------------------------------------------------ Best Weight Loss Program - Click Here! Weight Loss Program http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOA AYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 22 Date: Wed, 31 Mar 2010 09:39:46 -0500 From: Cheri Miller Subject: FW: [Histonet] cassette labels erased by processor To: "histonet-bounces@lists.utsouthwestern.edu" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" YES! That is why I use pencil only. I had a processor full of blank/ unlabeled cassettes because the lot# of the pens was bad. There is no way of knowing if the ink is reagent proof until a disaster like you have occurs. You don't always know if the ink lot passed its QC with absolute certainty. Pencil is fail proof. I hope your day gets better, Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Breen Sent: Wednesday, March 31, 2010 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labels erased by processor I PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 23 Date: Wed, 31 Mar 2010 15:40:44 +0100 From: Malika Benatti Subject: Re: [Histonet] cassette labels erased by processor To: Catherine Breen Cc: Histonet List Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes Hi there, I would suggest to use a pencil rather than a marker pen to label cassettes when processing cassette with the Sakura VIP , my lab had a number of the so called solvent proof pen on trial and have yet to find one that survive processing. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London " ... Smile it confuses people ..." On 31 Mar 2010, at 15:13, "Catherine Breen" wrote: > I am looking for help solving a lab mystery. The cassettes in our > lab are labeled with an SP Securline Marker II and then processed in > a Sakura VIP processor. Two weeks ago the entire batch came out > labeled much more lightly than usual, some to the point of where the > label was completely effaced. > Our current theory is that an acid cleanser (Citronox) or possibly > another acid was accidentally introduced into the processor. > Has any lab experienced this problem before, especially with Citronox? > > Thank you. > > ------------------------------------------------------------ > Best Weight Loss Program - Click Here! > Weight Loss Program > http://tagline.excite.com/c? > cp= > etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 45 **************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Wed Mar 31 10:52:14 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Mar 31 10:52:30 2010 Subject: [Histonet] RE: [BULK] Histonet Digest, Vol 76, Issue 45 In-Reply-To: <20100331144135.25F3942A986@mail1.pph.org> References: <20100331144135.25F3942A986@mail1.pph.org> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02862EE5@MAIL1.pph.local> For placentas, you will find that you get consistently good sections if you gross the placenta fresh, take the samples you will want from the cord, disc and make a membrane role (which is easily done if they are not fixed). Fix your samples overnight and trim for blocks the next day. For the membrane role, grab the free edge of the membrane with a large forceps, role the membrane up on the forceps toward the disc, stick two pins through the space between the forceps, and cut from disc, then slightly release grip on forceps and slide role into formalin cup. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 31, 2010 7:42 AM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 76, Issue 45 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Coverslipping with SubX (Vanessa Avalos) 2. Haunted cryostat/Thanks! (mtitford@aol.com) 3. GMS on decal tissue (Morken, Tim) 4. questions (Webb, Dorothy L) 5. Re: her2 validation (Pat Laurie) 6. FREE MONEY! (Jackie M O'Connor) 7. RE: questions (Garrison, Becky) 8. Thermo's Excelsior Tissue Processor Program's (Akemi Allison-Tacha) 9. re: cyto prep tech (Kim Tournear) 10. her-2 neu validation (Debbie Nannenga) 11. RE: questions (Tony Henwood) 12. RE: RE: Leica Paraplast (connie grubaugh) 13. (no subject) (Green JumpyOne) 14. Fwd: [Histonet] (no subject) (Malika Benatti) 15. RE: Thermo's Excelsior Tissue Processor Program's (Heckford, Karen - SMMC-SF) 16. RE: RE: Leica Paraplast (Nails, Felton) 17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett) 18. Texas Society for Histotechnology April 23-25, 2010 (kdwyer3322@aol.com) 19. cassette labels erased by processor (Catherine Breen) 20. RE: her2 validation (Weems, Joyce) 21. RE: cassette labels erased by processor (Sherwood, Margaret ) 22. FW: [Histonet] cassette labels erased by processor (Cheri Miller) 23. Re: cassette labels erased by processor (Malika Benatti) ---------------------------------------------------------------------- Message: 1 Date: Tue, 30 Mar 2010 11:15:38 -0700 From: "Vanessa Avalos" Subject: [Histonet] Coverslipping with SubX To: "'HISTONET LISTS'" Message-ID: <000001cad035$00d2f5b0$0278e110$@com> Content-Type: text/plain; charset="us-ascii" Is anyone using SubX ? I am trying it out and am having some difficulty coverslipping. I am using the Subx glue as directed since Acrymount didn't seem to work as well. I still get a hazy film under the glass and am getting big air bubbles as well. I can eventually get them out but its just takes a while and you know how time is precious when you have a line of slides to stain. The process is not as smooth as before. I really would like this to work out for me and eliminate xylene. Any suggestions?? Vanessa Fax: 602-277-2134 ------------------------------ Message: 2 Date: Tue, 30 Mar 2010 14:44:26 -0400 From: mtitford@aol.com Subject: [Histonet] Haunted cryostat/Thanks! To: Histonet@pathology.swmed.edu Message-ID: <8CC9E5028ADFB36-1500-2BE5@webmail-m010.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Thank you everyone who responded to my problem with a Leica CM 1850 cryostat turning itself off. About 11 people responded with tips. Thank you!! The Histonet is great!! In answer to some enquirys: 1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts for an hour. Jackie O' Conner asks if it is set to go back "on" after the defrost. I have no idea. It defrosts well the rest of the time, and turns itself back on. Brian Cornett-Early recommends changing the start device on the compressor. 2) Someone else asked if power is getting to the cryostat - Yes, when it turns itself off, the on/off switch is on "off". All you have to do is flip the switch and it starts pumping and cooling. 3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on the side of the compressor. That is probably where we will start. Mari Ann works for Leica so it sounds like good advice. Thank you everyone. Since it only breaks down about once a month, it will take a long time to determine if the problem is fixed. Michael Titford Pathology USA Mobile AL USA ------------------------------ Message: 3 Date: Tue, 30 Mar 2010 11:52:17 -0700 From: "Morken, Tim" Subject: [Histonet] GMS on decal tissue To: histonet Message-ID: <1AAF670737F193429070841C6B2ADD4C013B16C780@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Has anyone seen or heard of problems with Grocott Methenamine Silver staining for fungi on decal tissues? Specifically, background or false positives? I can't find anything in any books that gives any indications to not use decaled tissue. Thanks! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.morken@ucsfmedctr.org ------------------------------ Message: 4 Date: Tue, 30 Mar 2010 14:03:55 -0500 From: "Webb, Dorothy L" Subject: [Histonet] questions To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C5786C5846@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 5 Date: Tue, 30 Mar 2010 13:26:21 -0700 From: Pat Laurie Subject: Re: [Histonet] her2 validation To: anita dudley Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We left it up to our pathologist. Initially we did the 25 slide validation, our pathologist wasn't 100% convinced that he could read them accurately, so we did another 25, plus all of the ones we sent out which already had FISH ran. It ended up being almost 70 cases, and our pathologist was properly "educated" on how he should score them. He felt that there was excessive pressure put on the pathologists due to having to score these slides, not just say negative or positive. I'll say though for the last year or so, with about 6 her2 cases ran a day, he has been remarkably accurate. Any that he scores 2+ and has sent out for FISH he usually indicates if he thinks that it will be positive or negative. He has yet to be wrong. Another Pathologist who I talked to who helped set up the guidelines said that there isn't a "1 method fits all" process. Good luck. On Tue, Mar 30, 2010 at 7:19 AM, anita dudley wrote: > > sorry!!! I didn't put a subject in the previous post. wondering about how > many slides people are using for her2 validation. thanks > > > > anita dudley > > providence hospital > > mobile alabama > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your inbox. > > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com ------------------------------ Message: 6 Date: Tue, 30 Mar 2010 15:28:30 -0500 From: Jackie M O'Connor Subject: [Histonet] FREE MONEY! To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Now that I have your attention - - I would really like to hear from you if you are at all interested in a histology position at Abbott Laboratories in Northern Illinois. Even if you have applied previously. This is a great opportunity to work in a GLP research lab, a good deal of bench work, but developing IHC for toxicology purposes as well. We have a Biocare intelliPATH stainer - it's FUN! We have four full time technicians/technologists with a fifth open position. Abbott offers 3 weeks paid vacation, great benefits, and is a good company to work for. I've been here ten years, and I've never been anywhere 10 years. Please forward your resume to me, as well as go to www.Abbott.com to apply. Jackie O'Connor ------------------------------ Message: 7 Date: Tue, 30 Mar 2010 16:49:42 -0400 From: "Garrison, Becky" Subject: RE: [Histonet] questions To: "Webb, Dorothy L" , Message-ID: Content-Type: text/plain; charset="us-ascii" We receive the placentas fresh (they are refrigerated in L&D before transport to Pathology); add lots of formalin (use 163 oz containers) and let fix overnight. Early next morning, placenta is grossed and cassettes sit in formalin til end of day. This formalin is changed at least once so that bloody formalin is replaced with fresh. Placed on processor at end of second day after receipt. When we had L&D add formalin, there was never enough formalin added and the placentas sat unfixed at room temperature for long periods of time. This procedure works better for us. Yes, we can not meet the CAP guideline for 2 day TAT but do end up with a consistently better quality product for this tissue type. (Placentas make up a small portion of overall workload, so overall TAT is not affected). Prior to this procedure, placentas made up a disproportionate amount of reprocessed blocks. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, March 30, 2010 3:04 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 30 Mar 2010 16:04:19 -0700 (PDT) From: Akemi Allison-Tacha Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's To: histo net Message-ID: <465571.85431.qm@web113812.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All of you in Histoland, ? I am working?with a facility that recently purchased a Thermo Excelsior Tissue Processor.? They have had processing problems with several of their specimens using non-xylene clearing agent.? These issues were with both bx's and larger tissues.? The program and the non-xylene clearing agent was recommended by the technical staff at Thermo.? ? The histology staff?have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared. ? Also, the histotech's were using reagent alcohol, histological grade.? This grade of alcohol is denatured with methyl alcohol.? The sales representative was in and informed us that this type of alcohol?has damaging effects on the?instrument.? The representative will be coming in to flush out the system and reprogram the instrument next Monday.? ? I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with.? We are going to use basically the same program for the Excelsior, that we use on the VIP. ? I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs.? It would be great to?compare the reagents, programs,?timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs.? Thank you in advance for your assistance. Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ------------------------------ Message: 9 Date: Tue, 30 Mar 2010 16:05:40 -0700 (PDT) From: Kim Tournear Subject: [Histonet] re: cyto prep tech To: Histonet Message-ID: <839809.46245.qm@web54204.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Thank you to everyone who responded to my question about cyto prep tech work, ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! ------------------------------ Message: 10 Date: Tue, 30 Mar 2010 17:12:57 -0700 From: Debbie Nannenga Subject: [Histonet] her-2 neu validation To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Anita, We ran 25 amplified and 25 negative cases for our validation study. Good Luck. Debbie Nannenga, HTL(ASCP) QIHC InCyte Pathology Spokane, WA ------------------------------ Message: 11 Date: Wed, 31 Mar 2010 11:21:58 +1100 From: "Tony Henwood" Subject: RE: [Histonet] questions To: "Webb, Dorothy L" , Message-ID: Content-Type: text/plain; charset="us-ascii" Dorothy, Apart from hoping the blocks of tissue are not too thick, we microwave the cassettes in 10%NBF in a Milestone Mega TT - 2 hours at 45oC. This ensures adequate fixation prior to processing on a Shandon Excelsior Tissue Processor. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, 31 March 2010 6:04 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 12 Date: Tue, 30 Mar 2010 19:58:21 -0700 From: connie grubaugh Subject: RE: [Histonet] RE: Leica Paraplast To: , Message-ID: Content-Type: text/plain; charset="Windows-1252" Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference. Connie G. > Date: Tue, 23 Mar 2010 11:29:03 -0700 > From: ddreesen@sbcglobal.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Paraplast > > > Hi Kristen, > We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block. > > > From: histonet-request@lists.utsouthwestern.edu Message: 4 > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) > From: kristen arvidson > Subject: [Histonet] Leica Paraplast > To: histonet > Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Has anyone who uses paraplast (we use the basic one) noticed a change in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850552/direct/01/ ------------------------------ Message: 13 Date: Wed, 31 Mar 2010 01:52:42 -0700 From: Green JumpyOne Subject: [Histonet] (no subject) To: , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" http://www.trainedlabor.com/H6UH4Yh1UJ.html _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3 ------------------------------ Message: 14 Date: Wed, 31 Mar 2010 10:45:09 +0100 From: Malika Benatti Subject: Fwd: [Histonet] (no subject) To: Histonet List Message-ID: <8186A0A0-94D8-4E31-99D1-87B35FC3CDC6@gmail.com> Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes Dear Histonet list manager Is they anyway you could filter/block spam such as this one from be sent out to the Histonet list. Cheers Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London " ... Smile it confuses people ..." Begin forwarded message: > From: Green JumpyOne > Date: 31 March 2010 09:52:42 GMT+01:00 > To: , , > > Subject: [Histonet] (no subject) > > http://www.trainedlabor.com/H6UH4Yh1UJ.html > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your > inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 31 Mar 2010 05:02:26 -0700 From: "Heckford, Karen - SMMC-SF" Subject: RE: [Histonet] Thermo's Excelsior Tissue Processor Program's To: "Akemi Allison-Tacha" Cc: histonet@lists.utsouthwestern.edu Message-ID: <2842DC75AE43AA4B92954CFB31781BC105697F76@CHW-MSG-301.chw.edu> Content-Type: text/plain; charset="iso-8859-1" Pretty much any problems I have had with the Excelsior and I have been using one for 4 years now is with Pathologists loading it incorrectly. I use reagent alcohol in it all the time, with no problems. I prefer using xylene in my processor. Every single time I have switched and started using a non-xylene sub. I have had nothing but problems. If I use a non-xylene I save it for the stainer. Take it easy, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Tuesday, March 30, 2010 4:04 PM To: histo net Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's Hi All of you in Histoland, ? I am working?with a facility that recently purchased a Thermo Excelsior Tissue Processor.? They have had processing problems with several of their specimens using non-xylene clearing agent.? These issues were with both bx's and larger tissues.? The program and the non-xylene clearing agent was recommended by the technical staff at Thermo.? ? The histology staff?have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared. ? Also, the histotech's were using reagent alcohol, histological grade.? This grade of alcohol is denatured with methyl alcohol.? The sales representative was in and informed us that this type of alcohol?has damaging effects on the?instrument.? The representative will be coming in to flush out the system and reprogram the instrument next Monday.? ? I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with.? We are going to use basically the same program for the Excelsior, that we use on the VIP. ? I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs.? It would be great to?compare the reagents, programs,?timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs.? Thank you in advance for your assistance. Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 31 Mar 2010 07:27:34 -0500 From: "Nails, Felton" Subject: RE: [Histonet] RE: Leica Paraplast To: "'connie grubaugh'" , "ddreesen@sbcglobal.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii There is about three brands of paraplast and they all cut differently. In one of my labs I use paraplast plus and it ribbons very well however the blocks have to be colder then if you are using TissuePrep from Fisher. Leica may have sent you one of the other types of paraplast. (paraplast, Paraplast Plus, Paraplast Xtra) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: Tuesday, March 30, 2010 9:58 PM To: ddreesen@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Leica Paraplast Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference. Connie G. > Date: Tue, 23 Mar 2010 11:29:03 -0700 > From: ddreesen@sbcglobal.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Paraplast > > > Hi Kristen, > We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block. > > > From: histonet-request@lists.utsouthwestern.edu > Message: 4 > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) > From: kristen arvidson > Subject: [Histonet] Leica Paraplast > To: histonet > Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Has anyone who uses paraplast (we use the basic one) noticed a change in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850552/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ ------------------------------ Message: 17 Date: Wed, 31 Mar 2010 05:35:49 -0700 (PDT) From: Ann Bennett Subject: [Histonet] Thermo Fisher Excelsior Tissue Processor Programs To: histonet@lists.utsouthwestern.edu Message-ID: <106398.85301.qm@web114318.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 At one point we looked at getting an Excelsior and I spoke with our sales rep and he said all the reagents we use in our processor now are absolutely fine.? He mentioned nothing about not being able to use Reagent alcohols or xylene substitutes.? Hope this helps - have a happy histo day! ? ? ------------------------------ Message: 18 Date: Wed, 31 Mar 2010 08:42:06 -0400 From: kdwyer3322@aol.com Subject: [Histonet] Texas Society for Histotechnology April 23-25, 2010 To: histonet@lists.utsouthwestern.edu Message-ID: <8CC9EE6B49D9799-17C0-9D76@webmail-m051.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Histonetters, It is not too late to join us for the 2010 TSH meeting in Houston Texas April 23-25, 2010. The fun starts Thursday April 22, 2010 with a Golf outing open to all Vendors and Attendees. Workshops begin Friday April 23, 2010 at 8:00am. There is still plenty of time to register and get a hotel room to enjoy 2 full days of workshops and symposiums. If you would like a program go to txsh.org or contact me via this e-mail. Thanks, TSH Convention Committee ------------------------------ Message: 19 Date: Wed, 31 Mar 2010 10:13:25 -0400 From: "Catherine Breen" Subject: [Histonet] cassette labels erased by processor To: histonet@lists.utsouthwestern.edu Message-ID: <20100331101325.1573@web007.roc2.bluetie.com> Content-Type: text/plain; charset=UTF-8 I am looking for help solving a lab mystery. The cassettes in our lab are labeled with an SP Securline Marker II and then processed in a Sakura VIP processor. Two weeks ago the entire batch came out labeled much more lightly than usual, some to the point of where the label was completely effaced. Our current theory is that an acid cleanser (Citronox) or possibly another acid was accidentally introduced into the processor. Has any lab experienced this problem before, especially with Citronox? Thank you. ------------------------------------------------------------ Best Weight Loss Program - Click Here! Weight Loss Program http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= ------------------------------ Message: 20 Date: Wed, 31 Mar 2010 10:28:42 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: her2 validation To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16405E029D9@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" >From June-15, 2009 CAP checklist ANP.22997 Phase I N/A YES NO If the laboratory performs HER2 testing (HER2 protein over-expression by immunohistochemistry [IHC] or HER2 gene amplification by in situ hybridization [e.g. FISH, CISH*, SISH*, etc.]), has the laboratory documented appropriate validation for the assay(s)? NOTE: Initial test validation must be performed on a minimum of 25 cases (recommended 25-100). Validation may be performed by comparing the results of testing with a validated alternative method (i.e. IHC vs. FISH) either in the same laboratory or another laboratory, or with the same validated method performed in another laboratory; validation testing must be done using the same set of cases in both labs. If specimens are fixed in a medium other than 10% neutral buffered formalin, the validation study must show that results are concordant with results from formalin-fixed tissues. If significant changes are made in testing methods (e.g., antibody clone, antigen retrieval protocol or detection system, FISH probe or pretreatment protocol), revalidation is required. This checklist item applies to laboratories that perform the technical testing of specimens for HER2 amplification. Patient specimens should be fixed in the same manner as the specimens used for the validation study(ies). *CISH = chromogenic in-situ hybridization; SISH = silver-enhanced in-situ hybridization Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 21 Date: Wed, 31 Mar 2010 10:33:35 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] cassette labels erased by processor To: "Catherine Breen" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2414A@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" I had similar issues with all of the marking pens out there. We finally switched to Tissue-Tek Marking pencils #4160 and have not had a problem since. Plus they never "dry" out! Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Breen Sent: Wednesday, March 31, 2010 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labels erased by processor I am looking for help solving a lab mystery. The cassettes in our lab are labeled with an SP Securline Marker II and then processed in a Sakura VIP processor. Two weeks ago the entire batch came out labeled much more lightly than usual, some to the point of where the label was completely effaced. Our current theory is that an acid cleanser (Citronox) or possibly another acid was accidentally introduced into the processor. Has any lab experienced this problem before, especially with Citronox? Thank you. ------------------------------------------------------------ Best Weight Loss Program - Click Here! Weight Loss Program http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOA AYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 22 Date: Wed, 31 Mar 2010 09:39:46 -0500 From: Cheri Miller Subject: FW: [Histonet] cassette labels erased by processor To: "histonet-bounces@lists.utsouthwestern.edu" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" YES! That is why I use pencil only. I had a processor full of blank/ unlabeled cassettes because the lot# of the pens was bad. There is no way of knowing if the ink is reagent proof until a disaster like you have occurs. You don't always know if the ink lot passed its QC with absolute certainty. Pencil is fail proof. I hope your day gets better, Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Breen Sent: Wednesday, March 31, 2010 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labels erased by processor I PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 23 Date: Wed, 31 Mar 2010 15:40:44 +0100 From: Malika Benatti Subject: Re: [Histonet] cassette labels erased by processor To: Catherine Breen Cc: Histonet List Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes Hi there, I would suggest to use a pencil rather than a marker pen to label cassettes when processing cassette with the Sakura VIP , my lab had a number of the so called solvent proof pen on trial and have yet to find one that survive processing. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London " ... Smile it confuses people ..." On 31 Mar 2010, at 15:13, "Catherine Breen" wrote: > I am looking for help solving a lab mystery. The cassettes in our > lab are labeled with an SP Securline Marker II and then processed in > a Sakura VIP processor. Two weeks ago the entire batch came out > labeled much more lightly than usual, some to the point of where the > label was completely effaced. > Our current theory is that an acid cleanser (Citronox) or possibly > another acid was accidentally introduced into the processor. > Has any lab experienced this problem before, especially with Citronox? > > Thank you. > > ------------------------------------------------------------ > Best Weight Loss Program - Click Here! > Weight Loss Program > http://tagline.excite.com/c? > cp= > etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 45 **************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From napoli <@t> siscom.net Wed Mar 31 11:05:19 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Wed Mar 31 11:05:23 2010 Subject: [Histonet] <29A3CB81288E6F4BA2C9B3C8015A9A130176AC22@MD1EV002.medimmune.com> Message-ID: <4bb372bf.2ea.32e1.1115715446@siscom.net> I agree that these pens are excellent. Many years ago at the National Naval Medical center in Bethesda, MD we had an entire run of cassettes marked with "Xylene-proof" markers come out of the processor with all the ink dissolved and NOTHING on the cassettes! Fortunately, we had everything in order with a grossing log and were religious about keeping the order of grossed cassettes. This was a scary deal. SO I WILL NEVER TRUST THESE FOR CASSETTES, but I DO THINK that KP markers are great and the MOHS tech at the last practice and lab i worked for uses them. KP definitely good. I don't recommend any you havent used before...or test them first. A bad batch of ink and your entire run is blank. From Lise.Matzke <@t> hli.ubc.ca Wed Mar 31 11:21:52 2010 From: Lise.Matzke <@t> hli.ubc.ca (Lise Matzke) Date: Wed Mar 31 11:22:39 2010 Subject: [Histonet] retention of prosthetic valves Message-ID: <4BB3142F.F029.0016.1@hli.ubc.ca> Hi there, I was wondering what your hospital/centre's pathology policies indicate in terms of the time you retain prosthetic valve specimens? Months? Years? thanks for your feedback, Anne Marie ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From leiker <@t> buffalo.edu Wed Mar 31 11:40:55 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Mar 31 11:41:07 2010 Subject: [Histonet] RE: lab markers KP In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390E9BF7681D@IBMB7Exchange.digestivespecialists.com> References: <29A3CB81288E6F4BA2C9B3C8015A9A130176AC22@MD1EV002.medimmune.com> <5A2BD13465E061429D6455C8D6B40E390E9BF7681D@IBMB7Exchange.digestivespecialists.com> Message-ID: <128EF1E2E83A8ED95DFA6416@CDYwxp1931.ad.med.buffalo.edu> I agree as well! - I use them to mark everything, even things that don't get treated with chemicals. One note: they work best if you mark hard non-porous surfaces (like the plastic cassettes) the day before to give the ink time to bind with the material. Regards, Merced --On Wednesday, March 31, 2010 11:31 AM -0400 "Blazek, Linda" wrote: > I have to agree. They have never failed and work forever. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, > Joseph Sent: Wednesday, March 31, 2010 11:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] lab markers KP > > The only consistent lab pen I have used that will hold up under decal, > processing with various alcohol, xylene, hemo de is the KP marker plus. > I am sure there are several vendors, I use Mercedes. > > Nick Madary, HT/HTL(ASCP)QIHC > Medimmune Histology Mgr, > OMW, Area 4, Lab 2438 > 301.398.4745(vm) > 301.398.6360(lab) > 301.398.9745(fax) > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 31, 2010 > 10:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 76, Issue 45 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Coverslipping with SubX (Vanessa Avalos) > 2. Haunted cryostat/Thanks! (mtitford@aol.com) > 3. GMS on decal tissue (Morken, Tim) > 4. questions (Webb, Dorothy L) > 5. Re: her2 validation (Pat Laurie) > 6. FREE MONEY! (Jackie M O'Connor) > 7. RE: questions (Garrison, Becky) > 8. Thermo's Excelsior Tissue Processor Program's > (Akemi Allison-Tacha) > 9. re: cyto prep tech (Kim Tournear) > 10. her-2 neu validation (Debbie Nannenga) > 11. RE: questions (Tony Henwood) > 12. RE: RE: Leica Paraplast (connie grubaugh) > 13. (no subject) (Green JumpyOne) > 14. Fwd: [Histonet] (no subject) (Malika Benatti) > 15. RE: Thermo's Excelsior Tissue Processor Program's > (Heckford, Karen - SMMC-SF) > 16. RE: RE: Leica Paraplast (Nails, Felton) > 17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett) > 18. Texas Society for Histotechnology April 23-25, 2010 > (kdwyer3322@aol.com) > 19. cassette labels erased by processor (Catherine Breen) > 20. RE: her2 validation (Weems, Joyce) > 21. RE: cassette labels erased by processor (Sherwood, Margaret ) > 22. FW: [Histonet] cassette labels erased by processor (Cheri Miller) > 23. Re: cassette labels erased by processor (Malika Benatti) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 30 Mar 2010 11:15:38 -0700 > From: "Vanessa Avalos" > Subject: [Histonet] Coverslipping with SubX > To: "'HISTONET LISTS'" > Message-ID: <000001cad035$00d2f5b0$0278e110$@com> > Content-Type: text/plain; charset="us-ascii" > > Is anyone using SubX ? I am trying it out and am having some difficulty > coverslipping. I am using the Subx glue as directed since Acrymount didn't > seem to work as well. I still get a hazy film under the glass and am > getting big air bubbles as well. I can eventually get them out but its > just takes a while and you know how time is precious when you have a line > of slides to stain. The process is not as smooth as before. I really > would like this to work out for me and eliminate xylene. > > Any suggestions?? > > > > Vanessa > > Fax: 602-277-2134 > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 30 Mar 2010 14:44:26 -0400 > From: mtitford@aol.com > Subject: [Histonet] Haunted cryostat/Thanks! > To: Histonet@pathology.swmed.edu > Message-ID: <8CC9E5028ADFB36-1500-2BE5@webmail-m010.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > > Thank you everyone who responded to my problem with a Leica CM 1850 > cryostat turning itself off. About 11 people responded with tips. Thank > you!! The Histonet is great!! In answer to some enquirys: > 1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts > for an hour. Jackie O' Conner asks if it is set to go back "on" after the > defrost. I have no idea. It defrosts well the rest of the time, and turns > itself back on. Brian Cornett-Early recommends changing the start device > on the compressor. 2) Someone else asked if power is getting to the > cryostat - Yes, when it turns itself off, the on/off switch is on "off". > All you have to do is flip the switch and it starts pumping and cooling. > 3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on > the side of the compressor. That is probably where we will start. Mari > Ann works for Leica so it sounds like good advice. > > Thank you everyone. Since it only breaks down about once a month, it will > take a long time to determine if the problem is fixed. > > Michael Titford > Pathology USA > Mobile AL USA > > > > ------------------------------ > > Message: 3 > Date: Tue, 30 Mar 2010 11:52:17 -0700 > From: "Morken, Tim" > Subject: [Histonet] GMS on decal tissue > To: histonet > Message-ID: > > <1AAF670737F193429070841C6B2ADD4C013B16C780@EXMBMCB15.ucsfmedicalcenter.o > rg> > > Content-Type: text/plain; charset=us-ascii > > Has anyone seen or heard of problems with Grocott Methenamine Silver > staining for fungi on decal tissues? Specifically, background or false > positives? I can't find anything in any books that gives any indications > to not use decaled tissue. > > Thanks! > > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > Box 1656 > 1600 Divisadero St. > San Francisco, CA 94143-1656 > USA > > Phone: (415) 514-6042 > Pager: (415) 443-6509 > Fax: (415) 885-7409 > > Email: tim.morken@ucsfmedctr.org > > > > ------------------------------ > > Message: 4 > Date: Tue, 30 Mar 2010 14:03:55 -0500 > From: "Webb, Dorothy L" > Subject: [Histonet] questions > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > <65365F35C0F2EF4D846EC3CA73E49C43C5786C5846@HPEMX3.HealthPartners.int> > Content-Type: text/plain; charset="us-ascii" > > We have been having problems with underprocessed placental membrane, some > of which are cut fairly thick, but am seeing it on more than just the > thich samples. Does anyone out there in histoland have a special process > for placentas or any helpful hints? I do know that many times the > placentas sit without formalin in L&D for hours before they bring them to > histo and add formalin and this seems to me it could be a factor, even > though we have them sitting in formalin for a few hours before processing. > > Also, does anyone do the high-iron diamine stain for intestinal mucin > staining? Do you do it with an Alcian Blue-PAS stain? > > Thank you ahead of time for any and all responses!! > > Dorothy Webb, HT (ASCP) > Regions Histology Lab > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please > be advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is > strictly prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 > > > ------------------------------ > > Message: 5 > Date: Tue, 30 Mar 2010 13:26:21 -0700 > From: Pat Laurie > Subject: Re: [Histonet] her2 validation > To: anita dudley > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > We left it up to our pathologist. Initially we did the 25 slide > validation, our pathologist wasn't 100% convinced that he could read them > accurately, so we did another 25, plus all of the ones we sent out which > already had FISH ran. It ended up being almost 70 cases, and our > pathologist was properly "educated" on how he should score them. He felt > that there was excessive pressure put on the pathologists due to having > to score these slides, not just say negative or positive. I'll say > though for the last year or so, with about 6 her2 cases ran a day, he has > been remarkably accurate. Any that he scores 2+ and has sent out for > FISH he usually indicates if he thinks that it will be positive or > negative. He has yet to be wrong. Another Pathologist who I talked to > who helped set up the guidelines said that there isn't a "1 method fits > all" process. Good luck. > > On Tue, Mar 30, 2010 at 7:19 AM, anita dudley > wrote: > >> >> sorry!!! I didn't put a subject in the previous post. wondering about >> how many slides people are using for her2 validation. thanks >> >> >> >> anita dudley >> >> providence hospital >> >> mobile alabama >> >> _________________________________________________________________ >> The New Busy is not the old busy. Search, chat and e-mail from your >> inbox. >> >> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL: >> ON:WL:en-US:WM_HMP:032010_3_____________________________________________ >> __ Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Patrick Laurie HT(ASCP)QIHC > CellNetix Pathology & Laboratories > 1124 Columbia Street, Suite 200 > Seattle, WA 98104 > PH: 206-215-5949 > plaurie@cellnetix.com > > > ------------------------------ > > Message: 6 > Date: Tue, 30 Mar 2010 15:28:30 -0500 > From: Jackie M O'Connor > Subject: [Histonet] FREE MONEY! > To: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset="US-ASCII" > > Now that I have your attention - - > > I would really like to hear from you if you are at all interested in a > histology position at Abbott Laboratories in Northern Illinois. Even if > you have applied previously. > > This is a great opportunity to work in a GLP research lab, a good deal of > bench work, but developing IHC for toxicology purposes as well. We have > a Biocare intelliPATH stainer - it's FUN! > > We have four full time technicians/technologists with a fifth open > position. Abbott offers 3 weeks paid vacation, great benefits, and is a > good company to work for. I've been here ten years, and I've never been > anywhere 10 years. > > Please forward your resume to me, as well as go to www.Abbott.com to > apply. > > Jackie O'Connor > > > ------------------------------ > > Message: 7 > Date: Tue, 30 Mar 2010 16:49:42 -0400 > From: "Garrison, Becky" > Subject: RE: [Histonet] questions > To: "Webb, Dorothy L" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We receive the placentas fresh (they are refrigerated in L&D before > transport to Pathology); add lots of formalin (use 163 oz containers) > and let fix overnight. Early next morning, placenta is grossed and > cassettes sit in formalin til end of day. This formalin is changed at > least once so that bloody formalin is replaced with fresh. Placed on > processor at end of second day after receipt. > > When we had L&D add formalin, there was never enough formalin added and > the placentas sat unfixed at room temperature for long periods of time. > This procedure works better for us. Yes, we can not meet the CAP > guideline > for 2 day TAT but do end up with a consistently better quality product > for this tissue type. (Placentas make up a small portion of overall > workload, so overall TAT is not affected). Prior to this procedure, > placentas made > up a disproportionate amount of reprocessed blocks. > > Becky Garrison > Pathology Supervisor > Shands Jacksonville > Jacksonville, FL 32209 > 904-244-6237, phone > 904-244-4290, fax > 904-393-3194, pager > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Tuesday, March 30, 2010 3:04 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] questions > > We have been having problems with underprocessed placental membrane, > some of which are cut fairly thick, but am seeing it on more than just > the thich samples. Does anyone out there in histoland have a special > process for placentas or any helpful hints? I do know that many times > the placentas sit without formalin in L&D for hours before they bring > them to histo and add formalin and this seems to me it could be a > factor, even though we have them sitting in formalin for a few hours > before processing. > > Also, does anyone do the high-iron diamine stain for intestinal mucin > staining? Do you do it with an Alcian Blue-PAS stain? > > Thank you ahead of time for any and all responses!! > > Dorothy Webb, HT (ASCP) > Regions Histology Lab > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please > be advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is > strictly prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will > be reimbursed for reasonable costs incurred in notifying us. > HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Tue, 30 Mar 2010 16:04:19 -0700 (PDT) > From: Akemi Allison-Tacha > Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's > To: histo net > Message-ID: <465571.85431.qm@web113812.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi All of you in Histoland, > > I am working with a facility that recently purchased a Thermo Excelsior > Tissue Processor. They have had processing problems with several of > their specimens using non-xylene clearing agent. These issues were with > both bx's and larger tissues. The program and the non-xylene clearing > agent was recommended by the technical staff at Thermo. > > The histology staff have switched back to using xylene verses the > non-xylene clearing agent, and most of the issues have disappeared. > > Also, the histotech's were using reagent alcohol, histological grade. > This grade of alcohol is denatured with methyl alcohol. The sales > representative was in and informed us that this type of alcohol has > damaging effects on the instrument. The representative will be coming in > to flush out the system and reprogram the instrument next Monday. > > I looked over the current VIP program which is being used, and the > program, timing and temperatures are a little different from most > hospital and private laboratories I have worked with. We are going to > use basically the same program for the Excelsior, that we use on the VIP. > > I would like to know what other Excelsior Tissue Processor users that use > xylene have for their programs. It would be great to compare the > reagents, programs, timing, and temperatures for Routine overnight runs > and for Rapid Biopsy Runs. Thank you in advance for your assistance. > > Akemi Allison-Tacha BS, HT(ASCP)HTL > Director > Phoenix Lab Consulting > E-Mail: akemiat3377@yahoo.com > > > > > ------------------------------ > > Message: 9 > Date: Tue, 30 Mar 2010 16:05:40 -0700 (PDT) > From: Kim Tournear > Subject: [Histonet] re: cyto prep tech > To: Histonet > Message-ID: <839809.46245.qm@web54204.mail.re2.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Thank you to everyone who responded to my question about cyto prep tech > work, > > > > ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) > Histology Supervisor > Tucson Medical Center > Tucson, AZ > ~Don't let your life end before it begins~ > OU Rocks!!!! > > > > > ------------------------------ > > Message: 10 > Date: Tue, 30 Mar 2010 17:12:57 -0700 > From: Debbie Nannenga > Subject: [Histonet] her-2 neu validation > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hi Anita, > > > > > > We ran 25 amplified and 25 negative cases for our validation study. > > > > Good Luck. > > > > Debbie Nannenga, HTL(ASCP) QIHC > > InCyte Pathology > > Spokane, WA > > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 31 Mar 2010 11:21:58 +1100 > From: "Tony Henwood" > Subject: RE: [Histonet] questions > To: "Webb, Dorothy L" , > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Dorothy, > > Apart from hoping the blocks of tissue are not too thick, we microwave > the cassettes in 10%NBF in a Milestone Mega TT - 2 hours at 45oC. This > ensures adequate fixation prior to processing on a Shandon Excelsior > Tissue Processor. > > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Wednesday, 31 March 2010 6:04 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] questions > > > We have been having problems with underprocessed placental membrane, > some of which are cut fairly thick, but am seeing it on more than just > the thich samples. Does anyone out there in histoland have a special > process for placentas or any helpful hints? I do know that many times > the placentas sit without formalin in L&D for hours before they bring > them to histo and add formalin and this seems to me it could be a > factor, even though we have them sitting in formalin for a few hours > before processing. > > Also, does anyone do the high-iron diamine stain for intestinal mucin > staining? Do you do it with an Alcian Blue-PAS stain? > > Thank you ahead of time for any and all responses!! > > Dorothy Webb, HT (ASCP) > Regions Histology Lab > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please > be advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is > strictly prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will > be reimbursed for reasonable costs incurred in notifying us. > HealthPartners R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ************************************************************************* > ******** This email and any files transmitted with it are confidential > and intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient, please delete it > and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned > and although no computer viruses were detected, The Childrens Hospital at > Westmead accepts no liability for any consequential damage resulting from > email containing computer viruses. > ************************************************************************* > ******** > > > > ------------------------------ > > Message: 12 > Date: Tue, 30 Mar 2010 19:58:21 -0700 > From: connie grubaugh > Subject: RE: [Histonet] RE: Leica Paraplast > To: , > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > > Hi all, I questioned the Leica paraplast that we have received too. It > is real gummy and sticky. Takes me forever to cut. I asked and was > informed that it is the same stuff we have always got and there is no > difference. Except all of us techs have noticed a big difference. > > > > Connie G. > > > > > >> Date: Tue, 23 Mar 2010 11:29:03 -0700 >> From: ddreesen@sbcglobal.net >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] RE: Leica Paraplast >> >> >> Hi Kristen, >> We were using the Paraplast Xtra and switched to something else after we >> noticed a difference in the quality of the paraffin. The tissues weren't >> cutting as well and the paraffin seemed to be "gritty". We found we were >> going through many more blades due to nicks and scratches and many times >> had to switch blades mid-block. >> >> >> From: histonet-request@lists.utsouthwestern.edu >> > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) >> From: kristen arvidson >> Subject: [Histonet] Leica Paraplast >> To: histonet >> Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Has anyone who uses paraplast (we use the basic one) noticed a change in >> the quality of your tissue? I have recently found out that they have >> changed manufacturing sites in the past couple of months. I am having >> on and off issues with my skin specimens that have been going on for >> about 2 months or so. Thought there may be a correlation. Any thoughts? >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Hotmail: Trusted email with Microsoft's powerful SPAM protection. > http://clk.atdmt.com/GBL/go/210850552/direct/01/ > > ------------------------------ > > Message: 13 > Date: Wed, 31 Mar 2010 01:52:42 -0700 > From: Green JumpyOne > Subject: [Histonet] (no subject) > To: , , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > http://www.trainedlabor.com/H6UH4Yh1UJ.html > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:O > N:WL:en-US:WM_HMP:032010_3 > > ------------------------------ > > Message: 14 > Date: Wed, 31 Mar 2010 10:45:09 +0100 > From: Malika Benatti > Subject: Fwd: [Histonet] (no subject) > To: Histonet List > Message-ID: <8186A0A0-94D8-4E31-99D1-87B35FC3CDC6@gmail.com> > Content-Type: text/plain; charset=us-ascii; format=flowed; > delsp=yes > > Dear Histonet list manager > > Is they anyway you could filter/block spam such as this one from be > sent out to the Histonet list. > > Cheers > > Malika Benatti BSc MIBMS > Specialist Biomedical Scientist > Great Ormond Street Children Hospital > London > > " ... Smile it confuses people ..." > > Begin forwarded message: > >> From: Green JumpyOne >> Date: 31 March 2010 09:52:42 GMT+01:00 >> To: , , >> > > >> Subject: [Histonet] (no subject) >> > >> http://www.trainedlabor.com/H6UH4Yh1UJ.html >> >> _________________________________________________________________ >> The New Busy is not the old busy. Search, chat and e-mail from your >> inbox. >> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL: >> ON:WL:en-US:WM_HMP:032010_3_____________________________________________ >> __ Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 15 > Date: Wed, 31 Mar 2010 05:02:26 -0700 > From: "Heckford, Karen - SMMC-SF" > Subject: RE: [Histonet] Thermo's Excelsior Tissue Processor Program's > To: "Akemi Allison-Tacha" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <2842DC75AE43AA4B92954CFB31781BC105697F76@CHW-MSG-301.chw.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Pretty much any problems I have had with the Excelsior and I have been > using one for 4 years now is with Pathologists loading it incorrectly. I > use reagent alcohol in it all the time, with no problems. I prefer > using xylene in my processor. Every single time I have switched and > started using a non-xylene sub. I have had nothing but problems. If I > use a non-xylene I save it for the stainer. Take it easy, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi > Allison-Tacha Sent: Tuesday, March 30, 2010 4:04 PM > To: histo net > Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's > > Hi All of you in Histoland, > > I am working with a facility that recently purchased a Thermo Excelsior > Tissue Processor. They have had processing problems with several of > their specimens using non-xylene clearing agent. These issues were with > both bx's and larger tissues. The program and the non-xylene clearing > agent was recommended by the technical staff at Thermo. > > The histology staff have switched back to using xylene verses the > non-xylene clearing agent, and most of the issues have disappeared. > > Also, the histotech's were using reagent alcohol, histological grade. > This grade of alcohol is denatured with methyl alcohol. The sales > representative was in and informed us that this type of alcohol has > damaging effects on the instrument. The representative will be coming in > to flush out the system and reprogram the instrument next Monday. > > I looked over the current VIP program which is being used, and the > program, timing and temperatures are a little different from most > hospital and private laboratories I have worked with. We are going to > use basically the same program for the Excelsior, that we use on the VIP. > > I would like to know what other Excelsior Tissue Processor users that use > xylene have for their programs. It would be great to compare the > reagents, programs, timing, and temperatures for Routine overnight runs > and for Rapid Biopsy Runs. Thank you in advance for your assistance. > > Akemi Allison-Tacha BS, HT(ASCP)HTL > Director > Phoenix Lab Consulting > E-Mail: akemiat3377@yahoo.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 16 > Date: Wed, 31 Mar 2010 07:27:34 -0500 > From: "Nails, Felton" > Subject: RE: [Histonet] RE: Leica Paraplast > To: "'connie grubaugh'" , > "ddreesen@sbcglobal.net" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > tal.org> > > Content-Type: text/plain; charset=us-ascii > > There is about three brands of paraplast and they all cut differently. In > one of my labs I use paraplast plus and it ribbons very well however the > blocks have to be colder then if you are using TissuePrep from Fisher. > Leica may have sent you one of the other types of paraplast. (paraplast, > Paraplast Plus, Paraplast Xtra) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie > grubaugh Sent: Tuesday, March 30, 2010 9:58 PM > To: ddreesen@sbcglobal.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Leica Paraplast > > > Hi all, I questioned the Leica paraplast that we have received too. It > is real gummy and sticky. Takes me forever to cut. I asked and was > informed that it is the same stuff we have always got and there is no > difference. Except all of us techs have noticed a big difference. > > > > Connie G. > > > > > >> Date: Tue, 23 Mar 2010 11:29:03 -0700 >> From: ddreesen@sbcglobal.net >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] RE: Leica Paraplast >> >> >> Hi Kristen, >> We were using the Paraplast Xtra and switched to something else after we >> noticed a difference in the quality of the paraffin. The tissues weren't >> cutting as well and the paraffin seemed to be "gritty". We found we were >> going through many more blades due to nicks and scratches and many times >> had to switch blades mid-block. >> >> >> From: histonet-request@lists.utsouthwestern.edu >> > Message: 4 >> Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) >> From: kristen arvidson >> Subject: [Histonet] Leica Paraplast >> To: histonet >> Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Has anyone who uses paraplast (we use the basic one) noticed a change in >> the quality of your tissue? I have recently found out that they have >> changed manufacturing sites in the past couple of months. I am having >> on and off issues with my skin specimens that have been going on for >> about 2 months or so. Thought there may be a correlation. Any thoughts? >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Hotmail: Trusted email with Microsoft's powerful SPAM protection. > http://clk.atdmt.com/GBL/go/210850552/direct/01/_________________________ > ______________________ Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ============================================================ > > > > ------------------------------ > > Message: 17 > Date: Wed, 31 Mar 2010 05:35:49 -0700 (PDT) > From: Ann Bennett > Subject: [Histonet] Thermo Fisher Excelsior Tissue Processor Programs > To: histonet@lists.utsouthwestern.edu > Message-ID: <106398.85301.qm@web114318.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > At one point we looked at getting an Excelsior and I spoke with our sales > rep and he said all the reagents we use in our processor now are > absolutely fine. He mentioned nothing about not being able to use > Reagent alcohols or xylene substitutes. Hope this helps - have a happy > histo day! > > > > > > > ------------------------------ > > Message: 18 > Date: Wed, 31 Mar 2010 08:42:06 -0400 > From: kdwyer3322@aol.com > Subject: [Histonet] Texas Society for Histotechnology April 23-25, > 2010 > To: histonet@lists.utsouthwestern.edu > Message-ID: <8CC9EE6B49D9799-17C0-9D76@webmail-m051.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > Histonetters, > It is not too late to join us for the 2010 TSH meeting in Houston Texas > April 23-25, 2010. > > The fun starts Thursday April 22, 2010 with a Golf outing open to all > Vendors and Attendees. > > Workshops begin Friday April 23, 2010 at 8:00am. > > There is still plenty of time to register and get a hotel room to enjoy 2 > full days of workshops and symposiums. > > If you would like a program go to txsh.org or contact me via this e-mail. > > Thanks, > TSH Convention Committee > > > > > > ------------------------------ > > Message: 19 > Date: Wed, 31 Mar 2010 10:13:25 -0400 > From: "Catherine Breen" > Subject: [Histonet] cassette labels erased by processor > To: histonet@lists.utsouthwestern.edu > Message-ID: <20100331101325.1573@web007.roc2.bluetie.com> > Content-Type: text/plain; charset=UTF-8 > > I am looking for help solving a lab mystery. The cassettes in our lab > are labeled with an SP Securline Marker II and then processed in a Sakura > VIP processor. Two weeks ago the entire batch came out labeled much more > lightly than usual, some to the point of where the label was completely > effaced. Our current theory is that an acid cleanser (Citronox) or > possibly another acid was accidentally introduced into the processor. Has > any lab experienced this problem before, especially with Citronox? > > Thank you. > > ------------------------------------------------------------ > Best Weight Loss Program - Click Here! > Weight Loss Program > http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZ > mGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= > > ------------------------------ > > Message: 20 > Date: Wed, 31 Mar 2010 10:28:42 -0400 > From: "Weems, Joyce" > Subject: [Histonet] RE: her2 validation > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <92AD9B20A6C38C4587A9FEBE3A30E16405E029D9@CHEXCMS10.one.ads.che.org> > Content-Type: text/plain; charset="us-ascii" > > >> From June-15, 2009 CAP checklist > > > > ANP.22997 Phase I N/A YES NO > > If the laboratory performs HER2 testing (HER2 protein over-expression by > immunohistochemistry [IHC] or HER2 gene amplification by in situ > hybridization [e.g. FISH, CISH*, SISH*, etc.]), has the laboratory > documented appropriate validation for the assay(s)? > > NOTE: Initial test validation must be performed on a minimum of 25 cases > (recommended 25-100). Validation may be performed by comparing the > results of testing with a validated alternative method (i.e. IHC vs. > FISH) either in the same laboratory or another laboratory, or with the > same validated method performed in another laboratory; validation testing > must be done using the same set of cases in both labs. > > If specimens are fixed in a medium other than 10% neutral buffered > formalin, the validation study must show that results are concordant with > results from formalin-fixed tissues. > > If significant changes are made in testing methods (e.g., antibody > clone, antigen retrieval protocol or detection system, FISH probe or > pretreatment protocol), revalidation is required. > > This checklist item applies to laboratories that perform the technical > testing of specimens for HER2 amplification. Patient specimens should be > fixed in the same manner as the specimens used for the validation > study(ies). > > *CISH = chromogenic in-situ hybridization; SISH = silver-enhanced > in-situ hybridization > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > > > ------------------------------ > > Message: 21 > Date: Wed, 31 Mar 2010 10:33:35 -0400 > From: "Sherwood, Margaret " > Subject: RE: [Histonet] cassette labels erased by processor > To: "Catherine Breen" , > > Message-ID: > <073AE2BEA1C2BA4A8837AB6C4B943D9703E2414A@PHSXMB30.partners.org> > Content-Type: text/plain; charset="us-ascii" > > I had similar issues with all of the marking pens out there. We finally > switched to Tissue-Tek Marking pencils #4160 and have not had a problem > since. Plus they never "dry" out! > > Peggy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine > Breen Sent: Wednesday, March 31, 2010 10:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cassette labels erased by processor > > I am looking for help solving a lab mystery. The cassettes in our lab are > labeled with an SP Securline Marker II and then processed in a Sakura VIP > processor. Two weeks ago the entire batch came out labeled much more > lightly than usual, some to the point of where the label was completely > effaced. Our current theory is that an acid cleanser (Citronox) or > possibly another acid was accidentally introduced into the processor. > Has any lab experienced this problem before, especially with Citronox? > > Thank you. > > ------------------------------------------------------------ > Best Weight Loss Program - Click Here! > Weight Loss Program > http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZ > mGMQTOA AYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it > is addressed. If you believe this e-mail was sent to you in error and the > e-mail contains patient information, please contact the Partners > Compliance HelpLine at http://www.partners.org/complianceline . If the > e-mail was sent to you in error but does not contain patient information, > please contact the sender and properly dispose of the e-mail. > > > > > ------------------------------ > > Message: 22 > Date: Wed, 31 Mar 2010 09:39:46 -0500 > From: Cheri Miller > Subject: FW: [Histonet] cassette labels erased by processor > To: "histonet-bounces@lists.utsouthwestern.edu" > > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > > > YES! That is why I use pencil only. I had a processor full of blank/ > unlabeled cassettes because the lot# of the pens was bad. There is no way > of knowing if the ink is reagent proof until a disaster like you have > occurs. You don't always know if the ink lot passed its QC with absolute > certainty. Pencil is fail proof. I hope your day gets better, Cheri > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine > Breen Sent: Wednesday, March 31, 2010 9:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cassette labels erased by processor > > I > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If you are not the addressee intended / indicated or agent responsible > for delivering it to the addressee, you are hereby notified that you are > in possession of confidential and privileged information. Any > dissemination, distribution, or copying of this e-mail is strictly > prohibited. If you have received this message in error, please notify > the sender immediately and delete this email from your system. > > > > ------------------------------ > > Message: 23 > Date: Wed, 31 Mar 2010 15:40:44 +0100 > From: Malika Benatti > Subject: Re: [Histonet] cassette labels erased by processor > To: Catherine Breen > Cc: Histonet List > Message-ID: > Content-Type: text/plain; charset=us-ascii; format=flowed; > delsp=yes > > Hi there, > > I would suggest to use a pencil rather than a marker pen to label > cassettes when processing cassette with the Sakura VIP , my lab had a > number of the so called solvent proof pen on trial and have yet to > find one that survive processing. > > > > > Malika Benatti BSc MIBMS > Specialist Biomedical Scientist > Great Ormond Street Children Hospital > London > > " ... Smile it confuses people ..." > > On 31 Mar 2010, at 15:13, "Catherine Breen" wrote: > >> I am looking for help solving a lab mystery. The cassettes in our >> lab are labeled with an SP Securline Marker II and then processed in >> a Sakura VIP processor. Two weeks ago the entire batch came out >> labeled much more lightly than usual, some to the point of where the >> label was completely effaced. >> Our current theory is that an acid cleanser (Citronox) or possibly >> another acid was accidentally introduced into the processor. >> Has any lab experienced this problem before, especially with Citronox? >> >> Thank you. >> >> ------------------------------------------------------------ >> Best Weight Loss Program - Click Here! >> Weight Loss Program >> http://tagline.excite.com/c? >> cp= >> etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADN >> AAAAAAAAAAAAAAAAAAAEUlAqCWY= >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 76, Issue 45 > **************************************** > > > > To the extent this electronic communication or any of its attachments > contain information that is not in the public domain, such information is > considered by MedImmune to be confidential and proprietary. This > communication is expected to be read and/or used only by the > individual(s) for whom it is intended. If you have received this > electronic communication in error, please reply to the sender advising of > the error in transmission and delete the original message and any > accompanying documents from your system immediately, without copying, > reviewing or otherwise using them for any purpose. Thank you for your > cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From napoli <@t> siscom.net Wed Mar 31 11:51:47 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Wed Mar 31 11:51:53 2010 Subject: [Histonet] Message-ID: Message-ID: <4bb37da3.3e6.4886.355847169@siscom.net> I use nothing but SurgiPath (now LEICA) Blue Ribbon Paraffin. I run dermpath labs and this stuff is formulated for optimal skin sectioning. Highly recommend it....havent used anything else for 13 years. AB From JWeems <@t> sjha.org Wed Mar 31 11:55:40 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Mar 31 11:56:13 2010 Subject: [Histonet] retention of prosthetic valves In-Reply-To: <4BB3142F.F029.0016.1@hli.ubc.ca> References: <4BB3142F.F029.0016.1@hli.ubc.ca> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16405E029F8@CHEXCMS10.one.ads.che.org> We photograph and retain only if requested. If not requested, we discard with the regular specimens usually at least a month. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lise Matzke Sent: Wednesday, March 31, 2010 12:22 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] retention of prosthetic valves Hi there, I was wondering what your hospital/centre's pathology policies indicate in terms of the time you retain prosthetic valve specimens? Months? Years? thanks for your feedback, Anne Marie ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From tigger13b <@t> aol.com Wed Mar 31 12:50:53 2010 From: tigger13b <@t> aol.com (tigger13b@aol.com) Date: Wed Mar 31 12:51:07 2010 Subject: [Histonet] MM24 mounting media Message-ID: <8CC9F11D777A022-3274-20592@Webmail-m110.sysops.aol.com> Hello, Does anyone use MM24 mounting media from surgipath? We use cytoseal 60 but I can get the MM24 at a lower price. If anyone has used this, could you tell me whether you were satisfied with the product or not? Thanks so much. Brandi Higgins From rjr6 <@t> psu.edu Wed Mar 31 13:25:15 2010 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Wed Mar 31 13:25:32 2010 Subject: [Histonet] MM24 mounting media In-Reply-To: <8CC9F11D777A022-3274-20592@Webmail-m110.sysops.aol.com> References: <8CC9F11D777A022-3274-20592@Webmail-m110.sysops.aol.com> Message-ID: I use this and have had no problem with it. Roberta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tigger13b@aol.com Sent: Wednesday, March 31, 2010 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MM24 mounting media Hello, Does anyone use MM24 mounting media from surgipath? We use cytoseal 60 but I can get the MM24 at a lower price. If anyone has used this, could you tell me whether you were satisfied with the product or not? Thanks so much. Brandi Higgins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john <@t> imebinc.com Wed Mar 31 13:40:50 2010 From: john <@t> imebinc.com (John O'Brien) Date: Wed Mar 31 13:33:05 2010 Subject: [Histonet] RE: Histonet Digest, Vol 76, Issue 45 Message-ID: <000101cad101$b06ff460$4a01a8c0@EXECUTIVE01> Denise, Note#16 is a guy Named Felton Nails , I have done business with him in the past he at Children hospital in Texas, try calling him and see if you would try our paraffin, You can mention my name and let him know I said hello John -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 45 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Coverslipping with SubX (Vanessa Avalos) 2. Haunted cryostat/Thanks! (mtitford@aol.com) 3. GMS on decal tissue (Morken, Tim) 4. questions (Webb, Dorothy L) 5. Re: her2 validation (Pat Laurie) 6. FREE MONEY! (Jackie M O'Connor) 7. RE: questions (Garrison, Becky) 8. Thermo's Excelsior Tissue Processor Program's (Akemi Allison-Tacha) 9. re: cyto prep tech (Kim Tournear) 10. her-2 neu validation (Debbie Nannenga) 11. RE: questions (Tony Henwood) 12. RE: RE: Leica Paraplast (connie grubaugh) 13. (no subject) (Green JumpyOne) 14. Fwd: [Histonet] (no subject) (Malika Benatti) 15. RE: Thermo's Excelsior Tissue Processor Program's (Heckford, Karen - SMMC-SF) 16. RE: RE: Leica Paraplast (Nails, Felton) 17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett) 18. Texas Society for Histotechnology April 23-25, 2010 (kdwyer3322@aol.com) 19. cassette labels erased by processor (Catherine Breen) 20. RE: her2 validation (Weems, Joyce) 21. RE: cassette labels erased by processor (Sherwood, Margaret ) 22. FW: [Histonet] cassette labels erased by processor (Cheri Miller) 23. Re: cassette labels erased by processor (Malika Benatti) ---------------------------------------------------------------------- Message: 1 Date: Tue, 30 Mar 2010 11:15:38 -0700 From: "Vanessa Avalos" Subject: [Histonet] Coverslipping with SubX To: "'HISTONET LISTS'" Message-ID: <000001cad035$00d2f5b0$0278e110$@com> Content-Type: text/plain; charset="us-ascii" Is anyone using SubX ? I am trying it out and am having some difficulty coverslipping. I am using the Subx glue as directed since Acrymount didn't seem to work as well. I still get a hazy film under the glass and am getting big air bubbles as well. I can eventually get them out but its just takes a while and you know how time is precious when you have a line of slides to stain. The process is not as smooth as before. I really would like this to work out for me and eliminate xylene. Any suggestions?? Vanessa Fax: 602-277-2134 ------------------------------ Message: 2 Date: Tue, 30 Mar 2010 14:44:26 -0400 From: mtitford@aol.com Subject: [Histonet] Haunted cryostat/Thanks! To: Histonet@pathology.swmed.edu Message-ID: <8CC9E5028ADFB36-1500-2BE5@webmail-m010.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Thank you everyone who responded to my problem with a Leica CM 1850 cryostat turning itself off. About 11 people responded with tips. Thank you!! The Histonet is great!! In answer to some enquirys: 1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts for an hour. Jackie O' Conner asks if it is set to go back "on" after the defrost. I have no idea. It defrosts well the rest of the time, and turns itself back on. Brian Cornett-Early recommends changing the start device on the compressor. 2) Someone else asked if power is getting to the cryostat - Yes, when it turns itself off, the on/off switch is on "off". All you have to do is flip the switch and it starts pumping and cooling. 3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on the side of the compressor. That is probably where we will start. Mari Ann works for Leica so it sounds like good advice. Thank you everyone. Since it only breaks down about once a month, it will take a long time to determine if the problem is fixed. Michael Titford Pathology USA Mobile AL USA ------------------------------ Message: 3 Date: Tue, 30 Mar 2010 11:52:17 -0700 From: "Morken, Tim" Subject: [Histonet] GMS on decal tissue To: histonet Message-ID: <1AAF670737F193429070841C6B2ADD4C013B16C780@EXMBMCB15.ucsfmedicalcenter. org> Content-Type: text/plain; charset=us-ascii Has anyone seen or heard of problems with Grocott Methenamine Silver staining for fungi on decal tissues? Specifically, background or false positives? I can't find anything in any books that gives any indications to not use decaled tissue. Thanks! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.morken@ucsfmedctr.org ------------------------------ Message: 4 Date: Tue, 30 Mar 2010 14:03:55 -0500 From: "Webb, Dorothy L" Subject: [Histonet] questions To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C5786C5846@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 5 Date: Tue, 30 Mar 2010 13:26:21 -0700 From: Pat Laurie Subject: Re: [Histonet] her2 validation To: anita dudley Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We left it up to our pathologist. Initially we did the 25 slide validation, our pathologist wasn't 100% convinced that he could read them accurately, so we did another 25, plus all of the ones we sent out which already had FISH ran. It ended up being almost 70 cases, and our pathologist was properly "educated" on how he should score them. He felt that there was excessive pressure put on the pathologists due to having to score these slides, not just say negative or positive. I'll say though for the last year or so, with about 6 her2 cases ran a day, he has been remarkably accurate. Any that he scores 2+ and has sent out for FISH he usually indicates if he thinks that it will be positive or negative. He has yet to be wrong. Another Pathologist who I talked to who helped set up the guidelines said that there isn't a "1 method fits all" process. Good luck. On Tue, Mar 30, 2010 at 7:19 AM, anita dudley wrote: > > sorry!!! I didn't put a subject in the previous post. wondering > about how many slides people are using for her2 validation. thanks > > > > anita dudley > > providence hospital > > mobile alabama > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your > inbox. > > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAG > L:ON:WL:en-US:WM_HMP:032010_3___________________________________________ ____ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com ------------------------------ Message: 6 Date: Tue, 30 Mar 2010 15:28:30 -0500 From: Jackie M O'Connor Subject: [Histonet] FREE MONEY! To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Now that I have your attention - - I would really like to hear from you if you are at all interested in a histology position at Abbott Laboratories in Northern Illinois. Even if you have applied previously. This is a great opportunity to work in a GLP research lab, a good deal of bench work, but developing IHC for toxicology purposes as well. We have a Biocare intelliPATH stainer - it's FUN! We have four full time technicians/technologists with a fifth open position. Abbott offers 3 weeks paid vacation, great benefits, and is a good company to work for. I've been here ten years, and I've never been anywhere 10 years. Please forward your resume to me, as well as go to www.Abbott.com to apply. Jackie O'Connor ------------------------------ Message: 7 Date: Tue, 30 Mar 2010 16:49:42 -0400 From: "Garrison, Becky" Subject: RE: [Histonet] questions To: "Webb, Dorothy L" , Message-ID: Content-Type: text/plain; charset="us-ascii" We receive the placentas fresh (they are refrigerated in L&D before transport to Pathology); add lots of formalin (use 163 oz containers) and let fix overnight. Early next morning, placenta is grossed and cassettes sit in formalin til end of day. This formalin is changed at least once so that bloody formalin is replaced with fresh. Placed on processor at end of second day after receipt. When we had L&D add formalin, there was never enough formalin added and the placentas sat unfixed at room temperature for long periods of time. This procedure works better for us. Yes, we can not meet the CAP guideline for 2 day TAT but do end up with a consistently better quality product for this tissue type. (Placentas make up a small portion of overall workload, so overall TAT is not affected). Prior to this procedure, placentas made up a disproportionate amount of reprocessed blocks. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, March 30, 2010 3:04 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 30 Mar 2010 16:04:19 -0700 (PDT) From: Akemi Allison-Tacha Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's To: histo net Message-ID: <465571.85431.qm@web113812.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All of you in Histoland, ? I am working?with a facility that recently purchased a Thermo Excelsior Tissue Processor.? They have had processing problems with several of their specimens using non-xylene clearing agent.? These issues were with both bx's and larger tissues.? The program and the non-xylene clearing agent was recommended by the technical staff at Thermo.? ? The histology staff?have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared. ? Also, the histotech's were using reagent alcohol, histological grade.? This grade of alcohol is denatured with methyl alcohol.? The sales representative was in and informed us that this type of alcohol?has damaging effects on the?instrument.? The representative will be coming in to flush out the system and reprogram the instrument next Monday.? ? I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with.? We are going to use basically the same program for the Excelsior, that we use on the VIP. ? I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs.? It would be great to?compare the reagents, programs,?timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs.? Thank you in advance for your assistance. Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ------------------------------ Message: 9 Date: Tue, 30 Mar 2010 16:05:40 -0700 (PDT) From: Kim Tournear Subject: [Histonet] re: cyto prep tech To: Histonet Message-ID: <839809.46245.qm@web54204.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Thank you to everyone who responded to my question about cyto prep tech work, ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! ------------------------------ Message: 10 Date: Tue, 30 Mar 2010 17:12:57 -0700 From: Debbie Nannenga Subject: [Histonet] her-2 neu validation To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Anita, We ran 25 amplified and 25 negative cases for our validation study. Good Luck. Debbie Nannenga, HTL(ASCP) QIHC InCyte Pathology Spokane, WA ------------------------------ Message: 11 Date: Wed, 31 Mar 2010 11:21:58 +1100 From: "Tony Henwood" Subject: RE: [Histonet] questions To: "Webb, Dorothy L" , Message-ID: Content-Type: text/plain; charset="us-ascii" Dorothy, Apart from hoping the blocks of tissue are not too thick, we microwave the cassettes in 10%NBF in a Milestone Mega TT - 2 hours at 45oC. This ensures adequate fixation prior to processing on a Shandon Excelsior Tissue Processor. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, 31 March 2010 6:04 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ********* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ************************************************************************ ********* ------------------------------ Message: 12 Date: Tue, 30 Mar 2010 19:58:21 -0700 From: connie grubaugh Subject: RE: [Histonet] RE: Leica Paraplast To: , Message-ID: Content-Type: text/plain; charset="Windows-1252" Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference. Connie G. > Date: Tue, 23 Mar 2010 11:29:03 -0700 > From: ddreesen@sbcglobal.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Paraplast > > > Hi Kristen, > We were using the Paraplast Xtra and switched to something else after > we noticed a difference in the quality of the paraffin. The tissues > weren't cutting as well and the paraffin seemed to be "gritty". We > found we were going through many more blades due to nicks and > scratches and many times had to switch blades mid-block. > > > From: histonet-request@lists.utsouthwestern.edu > Message: 4 > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) > From: kristen arvidson > Subject: [Histonet] Leica Paraplast > To: histonet > Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Has anyone who uses paraplast (we use the basic one) noticed a change > in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft?s powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850552/direct/01/ ------------------------------ Message: 13 Date: Wed, 31 Mar 2010 01:52:42 -0700 From: Green JumpyOne Subject: [Histonet] (no subject) To: , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" http://www.trainedlabor.com/H6UH4Yh1UJ.html _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL: ON:WL:en-US:WM_HMP:032010_3 ------------------------------ Message: 14 Date: Wed, 31 Mar 2010 10:45:09 +0100 From: Malika Benatti Subject: Fwd: [Histonet] (no subject) To: Histonet List Message-ID: <8186A0A0-94D8-4E31-99D1-87B35FC3CDC6@gmail.com> Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes Dear Histonet list manager Is they anyway you could filter/block spam such as this one from be sent out to the Histonet list. Cheers Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London " ... Smile it confuses people ..." Begin forwarded message: > From: Green JumpyOne > Date: 31 March 2010 09:52:42 GMT+01:00 > To: , , > > > Subject: [Histonet] (no subject) > > http://www.trainedlabor.com/H6UH4Yh1UJ.html > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your > inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL: ON:WL:en-US:WM_HMP:032010_3_____________________________________________ __ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 31 Mar 2010 05:02:26 -0700 From: "Heckford, Karen - SMMC-SF" Subject: RE: [Histonet] Thermo's Excelsior Tissue Processor Program's To: "Akemi Allison-Tacha" Cc: histonet@lists.utsouthwestern.edu Message-ID: <2842DC75AE43AA4B92954CFB31781BC105697F76@CHW-MSG-301.chw.edu> Content-Type: text/plain; charset="iso-8859-1" Pretty much any problems I have had with the Excelsior and I have been using one for 4 years now is with Pathologists loading it incorrectly. I use reagent alcohol in it all the time, with no problems. I prefer using xylene in my processor. Every single time I have switched and started using a non-xylene sub. I have had nothing but problems. If I use a non-xylene I save it for the stainer. Take it easy, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Tuesday, March 30, 2010 4:04 PM To: histo net Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's Hi All of you in Histoland, ? I am working?with a facility that recently purchased a Thermo Excelsior Tissue Processor.? They have had processing problems with several of their specimens using non-xylene clearing agent.? These issues were with both bx's and larger tissues.? The program and the non-xylene clearing agent was recommended by the technical staff at Thermo.? ? The histology staff?have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared. ? Also, the histotech's were using reagent alcohol, histological grade.? This grade of alcohol is denatured with methyl alcohol.? The sales representative was in and informed us that this type of alcohol?has damaging effects on the?instrument.? The representative will be coming in to flush out the system and reprogram the instrument next Monday.? ? I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with.? We are going to use basically the same program for the Excelsior, that we use on the VIP. ? I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs.? It would be great to?compare the reagents, programs,?timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs.? Thank you in advance for your assistance. Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 31 Mar 2010 07:27:34 -0500 From: "Nails, Felton" Subject: RE: [Histonet] RE: Leica Paraplast To: "'connie grubaugh'" , "ddreesen@sbcglobal.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii There is about three brands of paraplast and they all cut differently. In one of my labs I use paraplast plus and it ribbons very well however the blocks have to be colder then if you are using TissuePrep from Fisher. Leica may have sent you one of the other types of paraplast. (paraplast, Paraplast Plus, Paraplast Xtra) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: Tuesday, March 30, 2010 9:58 PM To: ddreesen@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Leica Paraplast Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference. Connie G. > Date: Tue, 23 Mar 2010 11:29:03 -0700 > From: ddreesen@sbcglobal.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Paraplast > > > Hi Kristen, > We were using the Paraplast Xtra and switched to something else after > we noticed a difference in the quality of the paraffin. The tissues > weren't cutting as well and the paraffin seemed to be "gritty". We > found we were going through many more blades due to nicks and > scratches and many times had to switch blades mid-block. > > > From: histonet-request@lists.utsouthwestern.edu > Message: 4 > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) > From: kristen arvidson > Subject: [Histonet] Leica Paraplast > To: histonet > Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Has anyone who uses paraplast (we use the basic one) noticed a change > in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850552/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ ------------------------------ Message: 17 Date: Wed, 31 Mar 2010 05:35:49 -0700 (PDT) From: Ann Bennett Subject: [Histonet] Thermo Fisher Excelsior Tissue Processor Programs To: histonet@lists.utsouthwestern.edu Message-ID: <106398.85301.qm@web114318.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 At one point we looked at getting an Excelsior and I spoke with our sales rep and he said all the reagents we use in our processor now are absolutely fine.? He mentioned nothing about not being able to use Reagent alcohols or xylene substitutes.? Hope this helps - have a happy histo day! ? ? ------------------------------ Message: 18 Date: Wed, 31 Mar 2010 08:42:06 -0400 From: kdwyer3322@aol.com Subject: [Histonet] Texas Society for Histotechnology April 23-25, 2010 To: histonet@lists.utsouthwestern.edu Message-ID: <8CC9EE6B49D9799-17C0-9D76@webmail-m051.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Histonetters, It is not too late to join us for the 2010 TSH meeting in Houston Texas April 23-25, 2010. The fun starts Thursday April 22, 2010 with a Golf outing open to all Vendors and Attendees. Workshops begin Friday April 23, 2010 at 8:00am. There is still plenty of time to register and get a hotel room to enjoy 2 full days of workshops and symposiums. If you would like a program go to txsh.org or contact me via this e-mail. Thanks, TSH Convention Committee ------------------------------ Message: 19 Date: Wed, 31 Mar 2010 10:13:25 -0400 From: "Catherine Breen" Subject: [Histonet] cassette labels erased by processor To: histonet@lists.utsouthwestern.edu Message-ID: <20100331101325.1573@web007.roc2.bluetie.com> Content-Type: text/plain; charset=UTF-8 I am looking for help solving a lab mystery. The cassettes in our lab are labeled with an SP Securline Marker II and then processed in a Sakura VIP processor. Two weeks ago the entire batch came out labeled much more lightly than usual, some to the point of where the label was completely effaced. Our current theory is that an acid cleanser (Citronox) or possibly another acid was accidentally introduced into the processor. Has any lab experienced this problem before, especially with Citronox? Thank you. ------------------------------------------------------------ Best Weight Loss Program - Click Here! Weight Loss Program http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQui ZmGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= ------------------------------ Message: 20 Date: Wed, 31 Mar 2010 10:28:42 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: her2 validation To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16405E029D9@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" >From June-15, 2009 CAP checklist ANP.22997 Phase I N/A YES NO If the laboratory performs HER2 testing (HER2 protein over-expression by immunohistochemistry [IHC] or HER2 gene amplification by in situ hybridization [e.g. FISH, CISH*, SISH*, etc.]), has the laboratory documented appropriate validation for the assay(s)? NOTE: Initial test validation must be performed on a minimum of 25 cases (recommended 25-100). Validation may be performed by comparing the results of testing with a validated alternative method (i.e. IHC vs. FISH) either in the same laboratory or another laboratory, or with the same validated method performed in another laboratory; validation testing must be done using the same set of cases in both labs. If specimens are fixed in a medium other than 10% neutral buffered formalin, the validation study must show that results are concordant with results from formalin-fixed tissues. If significant changes are made in testing methods (e.g., antibody clone, antigen retrieval protocol or detection system, FISH probe or pretreatment protocol), revalidation is required. This checklist item applies to laboratories that perform the technical testing of specimens for HER2 amplification. Patient specimens should be fixed in the same manner as the specimens used for the validation study(ies). *CISH = chromogenic in-situ hybridization; SISH = silver-enhanced in-situ hybridization Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 21 Date: Wed, 31 Mar 2010 10:33:35 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] cassette labels erased by processor To: "Catherine Breen" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2414A@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" I had similar issues with all of the marking pens out there. We finally switched to Tissue-Tek Marking pencils #4160 and have not had a problem since. Plus they never "dry" out! Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Breen Sent: Wednesday, March 31, 2010 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labels erased by processor I am looking for help solving a lab mystery. The cassettes in our lab are labeled with an SP Securline Marker II and then processed in a Sakura VIP processor. Two weeks ago the entire batch came out labeled much more lightly than usual, some to the point of where the label was completely effaced. Our current theory is that an acid cleanser (Citronox) or possibly another acid was accidentally introduced into the processor. Has any lab experienced this problem before, especially with Citronox? Thank you. ------------------------------------------------------------ Best Weight Loss Program - Click Here! Weight Loss Program http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQui ZmGMQTOA AYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 22 Date: Wed, 31 Mar 2010 09:39:46 -0500 From: Cheri Miller Subject: FW: [Histonet] cassette labels erased by processor To: "histonet-bounces@lists.utsouthwestern.edu" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" YES! That is why I use pencil only. I had a processor full of blank/ unlabeled cassettes because the lot# of the pens was bad. There is no way of knowing if the ink is reagent proof until a disaster like you have occurs. You don't always know if the ink lot passed its QC with absolute certainty. Pencil is fail proof. I hope your day gets better, Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Breen Sent: Wednesday, March 31, 2010 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labels erased by processor I PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 23 Date: Wed, 31 Mar 2010 15:40:44 +0100 From: Malika Benatti Subject: Re: [Histonet] cassette labels erased by processor To: Catherine Breen Cc: Histonet List Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes Hi there, I would suggest to use a pencil rather than a marker pen to label cassettes when processing cassette with the Sakura VIP , my lab had a number of the so called solvent proof pen on trial and have yet to find one that survive processing. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London " ... Smile it confuses people ..." On 31 Mar 2010, at 15:13, "Catherine Breen" wrote: > I am looking for help solving a lab mystery. The cassettes in our > lab are labeled with an SP Securline Marker II and then processed in > a Sakura VIP processor. Two weeks ago the entire batch came out > labeled much more lightly than usual, some to the point of where the > label was completely effaced. > Our current theory is that an acid cleanser (Citronox) or possibly > another acid was accidentally introduced into the processor. > Has any lab experienced this problem before, especially with Citronox? > > Thank you. > > ------------------------------------------------------------ > Best Weight Loss Program - Click Here! > Weight Loss Program > http://tagline.excite.com/c? > cp= > etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADN AAAAAAAAAAAAAAAAAAAEUlAqCWY= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 45 **************************************** From Margaret.Perry <@t> sdstate.edu Wed Mar 31 13:46:01 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Mar 31 13:46:08 2010 Subject: [Histonet] GMS stain Message-ID: We sometimes have problems with the stain if we use positive slides. Margaret From Ronald.Houston <@t> nationwidechildrens.org Wed Mar 31 14:08:35 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Mar 31 14:09:05 2010 Subject: [Histonet] MM24 mounting media In-Reply-To: <8CC9F11D777A022-3274-20592@Webmail-m110.sysops.aol.com> References: <8CC9F11D777A022-3274-20592@Webmail-m110.sysops.aol.com> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB22669E5B2@chi2k3ms01.columbuschildrens.net> We use it both for manual and automated coverslipping with great results Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tigger13b@aol.com Sent: Wednesday, March 31, 2010 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MM24 mounting media Hello, Does anyone use MM24 mounting media from surgipath? We use cytoseal 60 but I can get the MM24 at a lower price. If anyone has used this, could you tell me whether you were satisfied with the product or not? Thanks so much. Brandi Higgins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From rsrichmond <@t> gmail.com Wed Mar 31 14:10:17 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Mar 31 14:10:21 2010 Subject: [Histonet] Re: retention of prosthetic valves Message-ID: Anne Marie (where? Perhaps in Canada, which is going to have different rules from the USA) >>was wondering what your hospital/centre's pathology policies indicate in terms of the time you retain prosthetic valve specimens? Months? Years?<< I don't know of any specific policies. I've had a Carbomedics aortic valve prosthesis now for 8 years. In 2005 a colleague of mine did an autopsy on a man who had one (and died of unrelated causes), and I took the opportunity to call Carbomedics to ask them if they wanted the valve, and if they'd want mine when the time comes. I was pleased to find out that they don't want them back, because they're working just fine. Bob Richmond Samurai Pathologist Knoxville TN From SHargrove <@t> urhcs.org Wed Mar 31 14:10:13 2010 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Wed Mar 31 14:10:42 2010 Subject: [Histonet] Haunted Cryostat In-Reply-To: Message-ID: We had the exact same problem that just started out of no where. One night I came in really late to do a post and went through a department that backed up to ours. Floor care was in there stripping the floors. The cryostat was off again when I went in the room. The next day we used that plug for our heat gun and flipped the cryostat off. They did not always use that plug, just had started after a desk had been moved. When they tripped the breaker they would reset it , but our machine had to be turned back on every time. We had a new plug installed that day. It would be nice if it was that simple for you. (Embedded image moved to file: pic05610.jpg) From malbenatti <@t> googlemail.com Wed Mar 31 14:20:29 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Wed Mar 31 14:20:36 2010 Subject: [Histonet] GMS stain In-Reply-To: References: Message-ID: Hi Margaret, What problem are you experiencing ? Are you over impregnated or under impregnated ? At what thickness do you cut your Section ? Ideally 3 to 4 microns How do you make up you Hexamine solution ? if you get contamination of Hexamine solution make sure that glass ware is clean. Try this. Make 0.75 g of hexamine in 100 ml coplin jar / leave it dissolve in distilled H2O at 60 oC while your slides are in chromic acid. When ready slowly ad 18 drops (approximately 1ml) of 5% silver nitrate, (make sure solution remain clear if cloudy, then glassware is contaminated and repeat this steps with chromic acid clean glassware) then add 2 ml of borax. Sliver solution should remain clear. add slides and check macroscopically slide after 5 to 7 mins, then at 5 mins interval after that. Depending on tissue, it should not take more that 15 mins to develop, then carry to next step. Hope this help Best wishes Malika Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Hospital London, UK On Wed, Mar 31, 2010 at 7:46 PM, Perry, Margaret wrote: > We sometimes have problems with the stain if we use positive slides. > Margaret > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " From dreynold <@t> mdanderson.org Wed Mar 31 14:25:16 2010 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Wed Mar 31 14:26:51 2010 Subject: [Histonet] RE: haunted Cryostat In-Reply-To: References: Message-ID: <785BBF0C5F49CE41BA74460A43A08F02167482BEB0@DCPWVMBXC0VS3.mdanderson.edu> We had the same problem with our Leica 3050S. It happened occasionally then became more and more frequent. We went through a lot of the same hoops you have gone through. When it shut down one day while I was cutting I knew it had to be a machine malfunction. Leica replaced something that was connected with the arm that holds the block and then goes to the power. This was about 13 months ago and I am afraid it is starting it again. I come in and the computer panel says "Power Failure" but it is still cooling. I think this is how it started out last time. In the beginning I figured we had had an electrical outage or someone was messing with it at night. ------------------------------ Message: 3 Date: Mon, 29 Mar 2010 15:20:35 -0400 From: mtitford@aol.com Subject: [Histonet] Is my Leica CM 1850 cryostat haunted? To: Histonet@pathology.swmed.edu Message-ID: <8CC9D8C0AA7A02F-B40-5D8F@webmail-m081.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" We have a Leica CM 1850 cryostat that about once every three weeks to a month, turnes itself off! Has anyone else experienced this? How do I fix it? Initially, we thought someone after hours was turning it off, maybe someone in housekeeping or a passing med tech. That was not the case. Later our Biomedical people replaced a capacitor or something in the bowels of the cryostat thinking that may be faulty and contribute to the problem. That did not help either. Still later, the cryostat was put on its own circuit (No help), and then after that, we tried an uninterruptible power source thinking minor power fluctuations were the cause (no help either). Anyone know how to fix this problem?. It is disconcerting to walk into the lab in the morning and the cryostat is at room temperature and the OR is going to have a busy day. Michael Titford Pathology USA Mobile AL ------------------------------ ***************** From b-frederick <@t> northwestern.edu Wed Mar 31 14:31:59 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Mar 31 14:32:54 2010 Subject: [Histonet] DAKO Her2 antibody Message-ID: <95D667DD0E6A41B0BABCA40660B5BF29@lurie.northwestern.edu> Anyone out there noticing problems with the Her2 antibody for Dako? Seems real strong and deteriorating quickly. We order it in lots, so you can imagine... Seems like every run is different. Same stainer, same titre , same tech. Tissue is from all over the country and world so we cannot control fixation etc. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From Janice.Mahoney <@t> alegent.org Wed Mar 31 14:44:13 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Mar 31 14:45:04 2010 Subject: [Histonet] New CAP grossing guidelines Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A043@EXCHMBC2.ad.ah.local> Is anyone concerned about the new (old) grossing personnel guidelines from CAP. Many labs use people to "process " tissue. No more! ANP.11610 Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. The CLIA regulations on high complexity testing personnel may be found at HC Testing Personnel. In addition, the CLIA regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at the above Web address and at Grandfathered Exceptions. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. Jan Mahoney Omaha, NE ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From jnocito <@t> satx.rr.com Wed Mar 31 16:08:11 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Mar 31 16:08:24 2010 Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A043@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A043@EXCHMBC2.ad.ah.local> Message-ID: <435C0FB8346642EE9327F76EB80EAFE7@JoePC> just had a lively discussion at work. My take is that the only thing CAP changed was that they combined the "processing" and "grossing" pieces together again, which I don't know why they split them in the first place. But you don't have the entire CAP note and many people miss this. The last item states OR three months of documented laboratory training in the high complexity area. Again, my take is that an unregistered histotech can have at least three months of documented training in grossing complex specimens, have the record signed off by the medical director and be ok. How far off am I? Joe ----- Original Message ----- From: "Mahoney,Janice A" To: "Histonet" Sent: Wednesday, March 31, 2010 2:44 PM Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines Is anyone concerned about the new (old) grossing personnel guidelines from CAP. Many labs use people to "process " tissue. No more! ANP.11610 Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. The CLIA regulations on high complexity testing personnel may be found at HC Testing Personnel. In addition, the CLIA regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at the above Web address and at Grandfathered Exceptions. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. Jan Mahoney Omaha, NE ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Wed Mar 31 16:10:26 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Mar 31 16:10:38 2010 Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines In-Reply-To: <435C0FB8346642EE9327F76EB80EAFE7@JoePC> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A043@EXCHMBC2.ad.ah.local> <435C0FB8346642EE9327F76EB80EAFE7@JoePC> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A044@EXCHMBC2.ad.ah.local> But above that after the education piece it says "in Addition". Jan, Omaha -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, March 31, 2010 4:08 PM To: Mahoney,Janice A; Histonet Subject: Re: [?? Probable Spam] [Histonet] New CAP grossing guidelines just had a lively discussion at work. My take is that the only thing CAP changed was that they combined the "processing" and "grossing" pieces together again, which I don't know why they split them in the first place. But you don't have the entire CAP note and many people miss this. The last item states OR three months of documented laboratory training in the high complexity area. Again, my take is that an unregistered histotech can have at least three months of documented training in grossing complex specimens, have the record signed off by the medical director and be ok. How far off am I? Joe ----- Original Message ----- From: "Mahoney,Janice A" To: "Histonet" Sent: Wednesday, March 31, 2010 2:44 PM Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines Is anyone concerned about the new (old) grossing personnel guidelines from CAP. Many labs use people to "process " tissue. No more! ANP.11610 Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. The CLIA regulations on high complexity testing personnel may be found at HC Testing Personnel. In addition, the CLIA regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at the above Web address and at Grandfathered Exceptions. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. Jan Mahoney Omaha, NE ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From kc <@t> ka-recruiting.com Wed Mar 31 16:54:03 2010 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Wed Mar 31 16:53:02 2010 Subject: [Histonet] ***NEW HISTOLOGY JOBS*** Message-ID: <788160102.1270072443320.JavaMail.cfservice@sl4app2> Are you interested in hearing about new job opportunities? I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. Below is a list of some of the Histology opportunities we are currently working on. New York, NY - Histotech 3rd shift Las Vegas, NV - Histotech 3rd shift Las Vegas, NV - Histology Supervisor - 3rd shift Palm Springs, CA - Histotech - 1st shift Los Angeles, CA - Histotech Northeastern, VA - Histotech 2nd shift Southern, NH - HTL and HT Atlanta, GA - Atlanta - Histotech Atlanta, GA - Atlanta - Pathology Coordinator (HT or CT) Southeastern, MA - IHC Tech Southeastern, MA - Histotech If you're interested in learning more about any of these opportunities then please email me a resume and let me know how best to get in touch with you. If none of these are a fit please let me know what you'd be interested in and where you're looking so I can tailor a search for you. With the New Year upon us many of our clients have fresh hiring budgets and will be looking to add people over the next several months. We work on positions at all levels and cover the entire US. To view some additional opportunities please visit our website at www.ka-recruiting.com . Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From vavalos <@t> allergydermatology.com Wed Mar 31 16:53:45 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Wed Mar 31 16:53:53 2010 Subject: FW: [Histonet] Coverslipping with SubX Message-ID: <000001cad11c$a47d3940$ed77abc0$@com> Sally, I added SubX to my acrymount since I have plenty of that in stock. It worked!! And I am so glad I can use up what I have instead of having to purchase a case of Sub X glue. My slides look clear and film free. I now am more confident in passing these slides over to be read. Thanks for all the helpful suggestions everyone! -----Original Message----- From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] Sent: Tuesday, March 30, 2010 11:25 AM To: Vanessa Avalos Subject: RE: [Histonet] Coverslipping with SubX Try thinning out the Sub-X-based mounting medium with some Sub-X. It sounds as if your mounting medium is too thick. Try that and see if it helps. You want your mounting medium to drip like cheap pancake syrup would drip - not too thick and not too thin. Too thick makes bubbles. I use Sub-X on my processor but use xylene on my autostainer, but that's because I have an tape coverslipper. I know you mean you're coverslipping by hand but see if the thinner medium helps you. Let me know! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From pathmaster <@t> yahoo.com Wed Mar 31 17:03:07 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Wed Mar 31 17:03:11 2010 Subject: [Histonet] Just three months????? Message-ID: <72644.18148.qm@web111105.mail.gq1.yahoo.com> CAP is now saying no more gross processing of small things that are? entirely submitted- it's all gross examination now whether we mean straining? currettings into a cassette or dissecting a complex cancer resection. Dumb as all get out if you ask me. As for the three month training thing, Joe, I'm not so sure about that. They seem to spell out specific amounts of college education required IN ADDITION to training in the laboratory. The requirement first spells out? two education choices- ?1: an earned associates degree in medical laboratory? science OR 2:Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education MUST (caps mine)? include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. So in addition to all the college courses, which we all know you need to count and measure six endoscopic biopsies and put them in a cassette, much more training and experience needed than what an unregistered on the job tech needs to orient and embed them properly LOL!!! ?? CLIA then requires? that the individual must have (additional)? laboratory training including either completion of? a clinical laboratory training program approved or accredited by the ABHES,? NAACLA, or other organization approved by HHS (note that this training may? be included in the 60 semester hours listed above), OR at least 3 months? documented laboratory training in each specialty in which the individual performs high complexity testing. Now there are grandfathering clauses in CLIA which may enable folks (like myself) to continue to gross. I haven't digested? that yet, but I'm ready to get that job in Pathmark if necessary. Sheesh, does it ever end? Jeff Silverman From Reuel.Cornelia <@t> tsrh.org Wed Mar 31 17:20:05 2010 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Wed Mar 31 17:20:29 2010 Subject: [Histonet] IHC detection for Pig tissue? Message-ID: <4BB38445020000C500074638@mail.TSRH.ORG> I just wanted to know if anybody who are working with Pig bone tissue fix in 10%NBF and decalcified in 14% EDTA, citrate buffer (dako) antigen retrieval pH7.0 and ph 9.0 by steaming for 20 minutes, what is their IHC detection kit for mouse monoclonal and rabbit polyclonal antibodies. I am using a Powervision kit and I have a lot of background staining even with my negative staining. Please help. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From andreahooper <@t> rocketmail.com Wed Mar 31 18:14:21 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Wed Mar 31 18:14:27 2010 Subject: [Histonet] mouse perfusion rate In-Reply-To: Message-ID: <619868.61085.qm@web113116.mail.gq1.yahoo.com> Very interesting! Coming out the nose is definitely bad for any work I have done in the past?- lungs get blown out, liver doesn't perfuse well and bone marrow looks horrific. However, if you are working with PFA and doing a post-fix anyway, you will probably be fine. If you are using GA and counting on the perfusion to ensure excellent fixation for things such as lacZ staining (b/c post-fix in GA never works well for bone or deep into tissues) then blowing it out the nose is bad. Very bad. Andrea ? --- On Mon, 3/29/10, Charles.Scouten@leica-microsystems.com wrote: From: Charles.Scouten@leica-microsystems.com Subject: RE: [Histonet] mouse perfusion rate To: leiker@buffalo.edu, saby_joseph_a@yahoo.com, making@ufl.edu, histonet@lists.utsouthwestern.edu Date: Monday, March 29, 2010, 6:26 PM I have perfused mice and rats at 300 mm Hg, about double physiological level, don't know what that made the flow rate.? All mammals have the same blood pressure (within tolerances), so it is easier to select a suitable pressure to use than a flow rate, which varies dramatically.? I look at brain, never pay any attention to the gut.? Clear fluid comes out the nose, that is a good sign.? There are pressure release valves across the cribiform plate to release CSF if there is too much.? I am flooding the system, fluid coming out the nose means the extracellular fluid and CSF is being replaced as well as vascular blood.? Good.? The tissue is quality is excellent, free of red blood cells, can be unshrunk depending on the tonicity (should be sub isotonic) of the fixative fluid.? Have looked at Nissl and EM material, no evidence of damage to the tissue. If gut is extended, might have something to do with the large intestines job of removing fluid from feces, and flooding the system swells the tissue.? But does it matter?? Do you use that tissue?? What is the tissue quality if you use it after physiological pressure perfusion. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: Merced M Leiker [mailto:Merced M Leiker ] Sent: Monday, March 29, 2010 9:05 AM To: Joseph Saby ; Charles.Scouten@leica-microsystems.com; making@ufl.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mouse perfusion rate Hi Joe, Thanks for that notice about flow rates. But I think for the mouse you meant 1-3mls/min (not per 10min?)... Regards, Merced --On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby < saby_joseph_a@yahoo.com> wrote: > > > All- > > From previous work with rat perfusions, the flow rate was about 10 > ml/minute. If I had to guess, the equivalent flow rate for a mouse would > be closer to 1-3 mls/10 minutes. If you go 10 ml/minute, you will > definitely cause blowout artefacts. > > Joe Saby, BA HT > > > > > __________________________________________________ > From: Merced M Leiker < leiker@buffalo.edu> > To: Charles.Scouten@leica-microsystems.com; making@ufl.edu; > histonet@lists.utsouthwestern.edu > Sent: Fri, March 19, 2010 9:21:38 AM > Subject: RE: [Histonet] mouse perfusion rate > > The vasculature will leak too much and the mouse will get bloated - > you'll > see it first in either the intestines blowing up like a balloon or fluid > coming out of the nose. Just not the same as the heart pumping when the > mouse is alive with intact physiology and normal functioning. Don't know > exactly why, but that's what happens when you go too fast. Perhaps the > vasculature has lost its control to compensate for the pressure? I'm not > a > physiologist so I'm not sure why...maybe someone on the Histonet can > answer > that? > > Regards, > Merced > > --On Thursday, March 18, 2010 5:49 PM -0500 > Charles.Scouten@leica-microsystems.com wrote: > >> >> >> Why not? What happens? One would think the mammalian cardiovascular >> system could withstand physiological pressures and flow rates, at least >> for one lifetime? >> >> >> >> >> Cordially, >> >> Charles W. Scouten, Ph.D >> >> Product Manager, MNL >> >> Biosystems Division >> >> >> >> Leica Biosystems Richmond, Inc. >> 5205 Route 12 >> P.O. Box 528 >> Richmond, IL 60071 >> United States of America >> >> Telephone 630 964 0501 >> >> facsimile +1 630 964 0576 >> >> www.MyNeuroLab.com >> >> www.leica-microsystems.com >> >> >> >> IMPORTANT - This email and any attachments may be confidential. Any >> retransmissions, dissemination or other use of >> >> these materials by persons or entities other than the intended recipient >> is prohibited. If received in error, please contact >> >> us and delete all copies. Before opening or using attachments, check them >> for viruses and defects. Our liability is limited >> >> to resupplying any affected attachments. [Any representations or opinions >> expressed in this email are those of the >> >> individual sender]. >> >> >> >> >> >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M >> Leiker < leiker@buffalo.edu> >> Sent: Thursday, March 18, 2010 12:38 PM >> To: MKing < making@ufl.edu>; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] mouse perfusion rate >> >> >> >> That may be mouse cardiac output, but I can assure you, from experience, >> you do not want to perfuse at 17ml/min. >> >> Regards, >> Merced >> >> --On Thursday, March 18, 2010 1:32 PM -0400 MKing < making@ufl.edu> >> wrote: >> >>> Li, >>> >>> Mouse cardiac output seems to be about 17 ml/min (e.g. >>> www.transonic.com/mice1.shtml), you probably want to try for that to >>> keep pressures close to physiological. >>> A syringe pump is pretty inexpensive and probably all you need. >>> >>> Mike >>> >>> ----- Original Message ----- >>> From: Li Zhang < dancingwing@yahoo.com> >>> Date: Wednesday, March 17, 2010 14:59 >>> Subject: [Histonet] question about mouse perfusion >>> To: histonet@lists.utsouthwestern.edu >>> >>> > > My question is: can anyone give me a rough idea of how fast I >>> > > should inject ( like ml/min). I think I've tried like 30 ml in 3 >>> > > min, and I suspect that it's too fast because I do observe >>> > > tissue swelling sometimes. >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> Merced M Leiker >> Research Technician III >> Cardiovascular Medicine >> 348 Biomedical Research Building >> State University of New York at Buffalo >> 3435 Main St, Buffalo, NY 14214 USA >> leiker@buffalo.edu >> 716-829-6118 (Ph) >> 716-829-2665 (Fx) >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> ______________________________________________________________________ >> This email has been scanned by the MessageLabs Email Security System. >> For more information please visit http://www.messagelabs.com/email >> ______________________________________________________________________ > > > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Mar 31 18:24:19 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Mar 31 18:24:23 2010 Subject: [Histonet] cassette labels erased by processor Message-ID: Indeed, One of the most EVIL products ever designed is the "Permanent Lab Marker" by VWR. Talk about false advertising! We have researchers drop off buckets of cassettes that they have labeled using these wonderful products. Pencil is the ONLY acceptable means of labeling cassettes. It is just not worth the risk to use anything else. (Except cassette labelers they are specifically designed for this and have a sales rep you can beat up if they fail.) Even pens specifically designed for cassettes have failed at times. Just my $0.02, Amos Message: 23 Date: Wed, 31 Mar 2010 15:40:44 +0100 From: Malika Benatti Subject: Re: [Histonet] cassette labels erased by processor To: Catherine Breen Cc: Histonet List Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes Hi there, I would suggest to use a pencil rather than a marker pen to label cassettes when processing cassette with the Sakura VIP , my lab had a number of the so called solvent proof pen on trial and have yet to find one that survive processing. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London