[Histonet] manual morphometric analysis

[mohamed abd el razik]

hi all histonetters
i need to do some morphomtric analysis on some samples of small intestin but we have no image analysis software so  i need to do it manually by occular micrometer.
i need to know how to calculate villus surface area? 
overall mucosal surface area of cross section?
 surface area of lamina propria?
mitotic figure index??
 
i don't know how to caculate all . any help please 
thanks in advance
 
mohamed
faculty of vet. med.
cairo univ.- egypt