[Histonet] pancreatin

[Emily Sours]

Hello histonetters!

I'm trying to make a pancreatin solution from Sigma pancreatin.  There is no
information with the actual product however, and the protocols we're
following are vague about what to dissolve it in.  They say to use a
"balanced salt solution without Ca 2+ and Mg 2+".  We assumed this is PBS
but the pancreatin (or the salts in the PBS) precipitated out when I added
it.  Does anyone have any idea what we could use?
We are using it for cleaning extra tissue off of chick or quail neural tube
transplants, if that helps.

Emily

Towns are like people. Old ones often have character, the new ones are
interchangeable.
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