[Histonet] porcine intervertebral disc
    Vicki Kalscheur 
    kalschev <@t> svm.vetmed.wisc.edu
       
    Fri Jun 18 10:16:47 CDT 2010
    
    
  
Jennifer,  If you can gently remove the disc, section appropriately and process for cross-section cuts you will get nice results. You might want to extend the paraffin times for very good infiltration.  If the disc/bone placement needs to be in place, it becomes more difficult as the decal is hard on the disc tissue and porcine bone take a long time to decal.  With disc/bone embedding and sectioning the soft and hard differences in the tissue are problematic. I suggest sectioning thicker.  It can be done and the results beautiful!   Vicki
    
    
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