[Histonet] cryojane question

[Kim Merriam]

Happy Friday!

We recently purchased a cryojane for sectioning frozen human and rat cartilage.  I am having some issues with my cartilage sections.  I am using the 4X slides with 2 flashes.  The sections look beautiful, until I put them into buffer to do staining.  I am getting folds and the sections are lifting off during IHC staining.  I am staining either by hand (on flat trays, old-fashioned style) or in the sequenza racks.  Luckily, the cartilage is not not coming off the slides completely, so I have been able to get the data I need.  I was wondering if anyone had any tips for me to prevent the folds or the lift-off.

Thanks,
Kim


 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA