From rstewart <@t> grmc.org Tue Jun 1 08:07:06 2010 From: rstewart <@t> grmc.org (Stewart, Robin P.) Date: Tue Jun 1 08:07:11 2010 Subject: [Histonet] thank you Message-ID: Good Morning Histotechers, I wanted to take a few minutes to say thank you to all who responded about my OJT trainees. This has been very helpful information and a big eye opener to my administration on how big a problem we have finding histo techs. From the bottom of my heart thank you all so much for your time. Have a great day. :) Robin Stewart From wdorsett-martin <@t> umc.edu Tue Jun 1 09:12:18 2010 From: wdorsett-martin <@t> umc.edu (Wanda A. Dorsett-Martin) Date: Tue Jun 1 09:12:28 2010 Subject: [Histonet] password Message-ID: <6E4AA1CF7F63E8499B665A73CF4F08150EFBA51E7E@EXMBX2.ntummc.umsmed.edu> How do I get a password? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From wdorsett-martin <@t> umc.edu Tue Jun 1 09:31:55 2010 From: wdorsett-martin <@t> umc.edu (Wanda A. Dorsett-Martin) Date: Tue Jun 1 09:32:05 2010 Subject: [Histonet] JB-4 Message-ID: <6E4AA1CF7F63E8499B665A73CF4F08150EFBA51E80@EXMBX2.ntummc.umsmed.edu> I have "inherited" a JB-4 manual microtome that was set up for plastic sectioning. I have no manual and I am not familiar with this machine. I would like to set it up for paraffin sectioning. Does anyone still have a manual for this type of microtome? It has a knife holder for metal disposable knives but I don't know what to do about the piece/pieces that hold the paraffin block. I really need a picture or a description of how the paraffin block is to be held for sectioning. Can anyone help? Thanks. Wanda Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From larry.reyes <@t> deaconessokc.com Tue Jun 1 09:58:35 2010 From: larry.reyes <@t> deaconessokc.com (Larry Reyes) Date: Tue Jun 1 09:58:40 2010 Subject: [Histonet] (no subject) Message-ID: <4FA371CA79EF864EB8068ADD6DC592B0120187@ok1568wxchg1.deaconessokc.org> We are considering purchasing a Lab Vision Autostainer. Does anyone have negative or positive feedback on this instrument. Thanks, Larry Reyes HT (ascp) ***************************** INFORMATION CONTAINED IN THIS MESSAGE MAY BE CONFIDENTIAL. If you have received this email in error, please contact the sender immediately. We also ask that you refrain from reading the contents of the message and delete it completely from your e-mail system. If you have printed a copy of the email, please destroy it immediately. From jmcgough <@t> clinlab.com Tue Jun 1 10:27:28 2010 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Tue Jun 1 10:27:42 2010 Subject: [Histonet] CMV Validation Slides In-Reply-To: Message-ID: We are wondering if anybody can help us with our CMV validation. We are needing 1 slide from 20 different known positive tissues. If you are able to help us or know of a source, please reply back to me. Thank you in advance for your help. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com From relia1 <@t> earthlink.net Tue Jun 1 10:29:34 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jun 1 10:29:44 2010 Subject: [Histonet] Hope you had a great Memorial Day Weekend - RELIA Histology Job Alert 06/01/10 Message-ID: Hi Histonetters!! I hope everyone had a fantastic Memorial Day Weekend ushering in Summer and appreciating our Military Families for the sacrifices they make to keep us safe and free. I wanted to take a minute after the holiday weekend to remind you of the positions that I am currently working on. All of these are full time permanent positions with reputable companies who offer excellent compensation, benefits and relocation assistance I have histology management positions in CA, AZ, NY and WA and Histotech positions in - AZ, MA, TX, MD, NY, CA and GA. If you or anyone you know is interested in more information on any of these positions I can be reached toll free at 866-607-3542 or relia1@earthliink.net. Have a great day - Thanks-Pam Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From POWELL_SA <@t> mercer.edu Tue Jun 1 11:52:21 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Jun 1 11:52:28 2010 Subject: [Histonet] Need help Message-ID: <9BF995BC0E47744E9673A41486E24EE2268C6A54E6@MERCERMAIL.MercerU.local> Hi Histonetters, One of my pathologists is going on a mission to Haiti in July. She is taking battery powered microscope to use. She is not sure what type of power is available. All the manual says about the battery option is "the LED configuration is operational with an in-built re-chargeable battery and charging circuit. The battery will be charged with a direct input power supply of 110-240V AC 50Hz/g0Hz which ensures continuous operation under fluctuating voltages." Greek to me. Has anyone been down since the earthquake using such battery powered scope? Know of power limitations? If so, got any tips, clues, pieces of wisdom? I know there are experts out there who know. Thanks, Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From carrie.schray <@t> gmail.com Tue Jun 1 13:14:38 2010 From: carrie.schray <@t> gmail.com (Carrie Schray) Date: Tue Jun 1 13:14:41 2010 Subject: [Histonet] Histology In Kenya Message-ID: Recently my laboratory received paraffin tissue blocks from Kenya. They were hand processed, and while sectioning we noticed an unusual chemical smell. I wondered if anyone could tell me what types of reagents are most often used for in Kenya for processing. Is ethanol, isopropanol, xylene, etc. used or even available in Kenya, or what other types of reagents may be used in place of ethanol, xylene, etc. Thank you, Carrie L. Schray, MA, HT(ASCP) Unit for Laboratory Animal Medicine University of Michigan Medical School Ann Arbor, Michigan From sgoebel <@t> xbiotech.com Tue Jun 1 14:29:19 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Jun 1 14:29:24 2010 Subject: [Histonet] Old stain row Message-ID: <20100601122919.9e2d9aa830e8449a2412eb1e4f2f067e.17e523d4c8.wbe@email04.secureserver.net> Hey all out there...just a quick question. Does anyone have Tissue Tek (or the likes) linear (unautomated) stain row (green and clear buckets) that they are possibly wanting to part with? Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotec 8201 East Riverside Dr. B Austin, Texas (512)386-5107 From denise.woodward <@t> uconn.edu Tue Jun 1 14:38:09 2010 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Tue Jun 1 14:38:24 2010 Subject: [Histonet] Job In New Hampshire Vet Path Lab Message-ID: I'm posting this for a friend. I know the Director and one of the Pathologists. They are super people who really appreciate and respect Histotechs. Univ. of New Hampshire Veterinary Diagnostic Lab has an opening for a Supervising Senior Histotechnologist. I've been told it's a nice little lab (I've never seen it), with room to grow (IHC autostainer recently donated, EM in the basement). The laboratory deals with on average 30-60 blocks/day, mostly biopsies, plus a handful of specials and necropsy cases, and some research projects. There is a part-time assistant, plus student help. >From the website: Summary of Position Under the direct supervision of NHVDL anatomical veterinary pathologist, the technologist in this position is responsible for managing the pathology section of the diagnostic laboratory including processing and supervising the processing of histologic specimens for evaluation by veterinary pathologists, training and supervising part-time hourly and workstudy laboratory assistants and providing histology support for research investigations. Acceptable Minimum Qualifications Bachelor's degree is preferred in Medical Technology or Animal/Biological Sciences, or Associates degree with two years of medical laboratory experience. Three years of Histotechnology experience required; ASCP-HT certification preferred. Here's the link to the UNH HR website if you want more information. https://jobs.usnh.edu/applicants/jsp/shared/position/JobDetails_css.jsp?postingId=138272 From rjbuesa <@t> yahoo.com Tue Jun 1 14:40:25 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 1 14:40:28 2010 Subject: [Histonet] Histology In Kenya In-Reply-To: Message-ID: <213789.50120.qm@web65702.mail.ac4.yahoo.com> I really think that this question should be asked to whomever sent you the blocks. In Histonet would be only a guess. Ren? J. --- On Tue, 6/1/10, Carrie Schray wrote: From: Carrie Schray Subject: [Histonet] Histology In Kenya To: histonet@lists.utsouthwestern.edu Date: Tuesday, June 1, 2010, 2:14 PM Recently my laboratory received paraffin tissue blocks from Kenya. They were hand processed, and while sectioning we noticed an unusual chemical smell. I wondered if anyone could tell me what types of reagents are most often used for in Kenya for processing. Is ethanol, isopropanol, xylene, etc. used or even available in Kenya, or what other types of reagents may be used in place of ethanol, xylene, etc. Thank you, Carrie L. Schray, MA, HT(ASCP) Unit for Laboratory Animal Medicine University of Michigan Medical School Ann Arbor, Michigan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Jun 1 15:07:46 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jun 1 15:07:55 2010 Subject: [Histonet] Review of outside Path Message-ID: <4C053052.7400.0077.1@harthosp.org> For those of you working in a hospital pathology laboratory, do you have a policy requiring review of outside pathology slides from patients diagnosed with cancer before that patient has surgery, chemotherapy, or radiation therapy at your institution? If so, is it a hospital or departmental policy? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From rjbuesa <@t> yahoo.com Tue Jun 1 15:42:36 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 1 15:42:40 2010 Subject: [Histonet] Review of outside Path In-Reply-To: <4C053052.7400.0077.1@harthosp.org> Message-ID: <370066.57519.qm@web65701.mail.ac4.yahoo.com> We did not have such a policy. Patient's slides were reviewed only as a consultation sent by their attending pathologists. Ren? J. --- On Tue, 6/1/10, Richard Cartun wrote: From: Richard Cartun Subject: [Histonet] Review of outside Path To: "Histonet" Date: Tuesday, June 1, 2010, 4:07 PM For those of you working in a hospital pathology laboratory, do you have a policy requiring review of outside pathology slides from patients diagnosed with cancer before that patient has surgery, chemotherapy, or radiation therapy at your institution?? If so, is it a hospital or departmental policy?? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT? 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Scouten <@t> leica-microsystems.com Tue Jun 1 16:42:12 2010 From: Charles.Scouten <@t> leica-microsystems.com (Charles.Scouten@leica-microsystems.com) Date: Tue Jun 1 16:42:33 2010 Subject: [Histonet] Vibrotoming otoliths Message-ID: Carve a channel in balsa wood, available at hobby shops, and sandwich the otolith in. Section wood and all. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jason christian Sent: Wednesday, May 26, 2010 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Vibrotoming otoliths I am trying to section an otolith from a small minnow species with a vibrotome. The bone seems to be very dense and doesn't hold very well inside any mounting media that I have tried. Does anyone have any suggestions as to a media that will section smoothly but would be dense enough to hold the small bone in the media while its being sectioned. Thank you in advance. Jason _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From cwmynot <@t> hotmail.com Tue Jun 1 16:46:49 2010 From: cwmynot <@t> hotmail.com (chad miller) Date: Tue Jun 1 16:46:58 2010 Subject: [Histonet] RE: Histonet Digest, Vol 79, Issue 1 In-Reply-To: References: Message-ID: Larry, Look at the Biocare Nemesis 7200. We switched from the LabVision instrument in 2007 to the Biocare Nemesis in 2007 and have been pleased with its performance. We do not run a wide variety of antibodies but find this stainer easy to use, flexible and consistent. Thanks, Chad Miller HT ASCP > From: histonet-request@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 79, Issue 1 > To: histonet@lists.utsouthwestern.edu > Date: Tue, 1 Jun 2010 10:41:47 -0700 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. thank you (Stewart, Robin P.) > 2. password (Wanda A. Dorsett-Martin) > 3. JB-4 (Wanda A. Dorsett-Martin) > 4. (no subject) (Larry Reyes) > 5. CMV Validation Slides (Jason McGough) > 6. Hope you had a great Memorial Day Weekend - RELIA Histology > Job Alert 06/01/10 (Pam Barker) > 7. Need help (Shirley A. Powell) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 1 Jun 2010 07:07:06 -0600 > From: "Stewart, Robin P." > Subject: [Histonet] thank you > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Good Morning Histotechers, > I wanted to take a few minutes to say thank you to all who responded about my OJT trainees. This has been very helpful information and a big eye opener to my administration on how big a problem we have finding histo techs. >From the bottom of my heart thank you all so much for your time. Have a great day. :) > Robin Stewart > > > ------------------------------ > > Message: 2 > Date: Tue, 1 Jun 2010 09:12:18 -0500 > From: "Wanda A. Dorsett-Martin" > Subject: [Histonet] password > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <6E4AA1CF7F63E8499B665A73CF4F08150EFBA51E7E@EXMBX2.ntummc.umsmed.edu> > Content-Type: text/plain; charset="us-ascii" > > How do I get a password? > > > Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. > > > > ------------------------------ > > Message: 3 > Date: Tue, 1 Jun 2010 09:31:55 -0500 > From: "Wanda A. Dorsett-Martin" > Subject: [Histonet] JB-4 > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <6E4AA1CF7F63E8499B665A73CF4F08150EFBA51E80@EXMBX2.ntummc.umsmed.edu> > Content-Type: text/plain; charset="us-ascii" > > I have "inherited" a JB-4 manual microtome that was set up for plastic sectioning. I have no manual and I am not familiar with this machine. I would like to set it up for paraffin sectioning. Does anyone still have a manual for this type of microtome? It has a knife holder for metal disposable knives but I don't know what to do about the piece/pieces that hold the paraffin block. I really need a picture or a description of how the paraffin block is to be held for sectioning. Can anyone help? Thanks. > Wanda > > > Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. > > > > ------------------------------ > > Message: 4 > Date: Tue, 1 Jun 2010 09:58:35 -0500 > From: "Larry Reyes" > Subject: [Histonet] (no subject) > To: > Message-ID: > <4FA371CA79EF864EB8068ADD6DC592B0120187@ok1568wxchg1.deaconessokc.org> > Content-Type: text/plain; charset="us-ascii" > > > We are considering purchasing a Lab Vision Autostainer. > Does anyone have negative or positive feedback on this instrument. > > Thanks, > > Larry Reyes HT (ascp) > > ***************************** > > INFORMATION CONTAINED IN THIS MESSAGE MAY BE CONFIDENTIAL. > If you have received this email in error, please contact the sender > immediately. We also ask that you refrain from reading the contents > of the message and delete it completely from your e-mail system. If > you have printed a copy of the email, please destroy it immediately. > > > > > ------------------------------ > > Message: 5 > Date: Tue, 1 Jun 2010 09:27:28 -0600 > From: "Jason McGough" > Subject: [Histonet] CMV Validation Slides > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > We are wondering if anybody can help us with our CMV validation. We are > needing 1 slide from 20 different known positive tissues. If you are able to > help us or know of a source, please reply back to me. Thank you in advance > for your help. > > Jason McGough HT(ASCP) > Account Representative - Anatomic Pathology > Clinical Laboratory of the Black Hills > 2805 5th Street Suite 210 > Rapid City, SD 57701 > 605-343-2267 Ext 127 > 605-718-3779 (Fax) > jmcgough@clinlab.com > > > > > > > > > ------------------------------ > > Message: 6 > Date: Tue, 1 Jun 2010 11:29:34 -0400 > From: "Pam Barker" > Subject: [Histonet] Hope you had a great Memorial Day Weekend - RELIA > Histology Job Alert 06/01/10 > To: "'Histonet'" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters!! > I hope everyone had a fantastic Memorial Day Weekend ushering in Summer > and appreciating our Military Families for the sacrifices they make to > keep us safe and free. > I wanted to take a minute after the holiday weekend to remind you of the > positions that I am currently working on. All of these are full time > permanent positions with reputable companies who offer excellent > compensation, benefits and relocation assistance I have histology > management positions in CA, AZ, NY and WA and Histotech positions in - > AZ, MA, TX, MD, NY, CA and GA. > > If you or anyone you know is interested in more information on any of > these positions I can be reached toll free at 866-607-3542 or > relia1@earthliink.net. > > Have a great day - Thanks-Pam > > Thank You! > > Pam Barker > President > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > Toll Free: (866)607-3542 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > <> > www.myspace.com/pamatrelia > www.twitter.com/pamatrelia > > > > ------------------------------ > > Message: 7 > Date: Tue, 1 Jun 2010 12:52:21 -0400 > From: "Shirley A. Powell" > Subject: [Histonet] Need help > To: histonet > Message-ID: > <9BF995BC0E47744E9673A41486E24EE2268C6A54E6@MERCERMAIL.MercerU.local> > Content-Type: text/plain; charset="us-ascii" > > Hi Histonetters, > > One of my pathologists is going on a mission to Haiti in July. She is taking battery powered microscope to use. She is not sure what type of power is available. All the manual says about the battery option is "the LED configuration is operational with an in-built re-chargeable battery and charging circuit. The battery will be charged with a direct input power supply of 110-240V AC 50Hz/g0Hz which ensures continuous operation under fluctuating voltages." Greek to me. Has anyone been down since the earthquake using such battery powered scope? Know of power limitations? If so, got any tips, clues, pieces of wisdom? I know there are experts out there who know. > > Thanks, > > > Shirley A. Powell, HT(ASCP)HTL, QIHC > Technical Director > Histology Curricular Support Laboratory > Mercer University School of Medicine > 1550 College Street > Macon, GA 31207 > 478-301-2374 Lab > 478-301-5489 Fax > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 79, Issue 1 > *************************************** _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From gguerzon <@t> live.com Tue Jun 1 19:57:43 2010 From: gguerzon <@t> live.com (Godfrey Guerzon) Date: Tue Jun 1 19:57:48 2010 Subject: [Histonet] Review of outside Path In-Reply-To: <370066.57519.qm@web65701.mail.ac4.yahoo.com> References: <4C053052.7400.0077.1@harthosp.org>, <370066.57519.qm@web65701.mail.ac4.yahoo.com> Message-ID: If I am not mistaken, at a hospital I used to work, I remember reviewing a policy prior to our CAP inspection that states patients for surgery, chemotherapy and radiation therapy prior to treatment at this hospital where the diagnosis was made at another institution, slides are requested and reviewed by our Pathologists prior to treatment. I will have to follow-up with the laboratory director and find out if it is a department policy or a hospital policy. Godfrey > Date: Tue, 1 Jun 2010 13:42:36 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; Rcartun@harthosp.org > Subject: Re: [Histonet] Review of outside Path > CC: > > We did not have such a policy. Patient's slides were reviewed only as a consultation sent by their attending pathologists. > Ren? J. > > --- On Tue, 6/1/10, Richard Cartun wrote: > > > From: Richard Cartun > Subject: [Histonet] Review of outside Path > To: "Histonet" > Date: Tuesday, June 1, 2010, 4:07 PM > > > For those of you working in a hospital pathology laboratory, do you have a policy requiring review of outside pathology slides from patients diagnosed with cancer before that patient has surgery, chemotherapy, or radiation therapy at your institution? If so, is it a hospital or departmental policy? Thank you. > > Richard > > Richard W. Cartun, Ph.D. > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2 From eberledelphine <@t> hotmail.com Tue Jun 1 20:49:29 2010 From: eberledelphine <@t> hotmail.com (delphine eberle) Date: Tue Jun 1 20:49:33 2010 Subject: [Histonet] Oil Red O staining for atherosclerosis lesions Message-ID: Hi, We stain frozen aortic root sections with oil red o (diluted in propylen glycol) as follow: 1. Fix slides in 10% formalin (5 min) 2. Rinse in 1X PBS (5 min) 3. Dip in Distilled H2O until clear (3 dips) 4. Transfer slides into 100% propylene glycol (2 min) 5. Transfer slides into 0.5% Oil Red O stain (overnight) (Filter before use) 6. Differentiate slides in 85% aqueous propylene glycol (2 min) 7. Wash slides in distilled H2O (3 min) 8. Counter-stain nuclei in Hematoxylin Gil 1X (2min) 9. Dip in Tap H2O until clear (3 dips) 10. Bluing Reagent (0.1% Sodium Bicarbonate in distilled H2O) (3-4 dips) 11. Tap H2O (30 sec) 12. Coverslip and seal slides using Aquatex mounting medium Recently, we've been getting dirty staining with multiple droplets left on the slides. We tryied to do more washes in 85% propylene glycol but it does not seem to improve. Would anybody have ideas to solve this? Thanks!! Delphine Delphine Eberl? PhD UCSF Department of Vascular Surgery VA Medical Center - NCIRE Building 2 - room 410 4150 Clement Street San Francisco, CA 94121, USA Tel: 415 221 4810 ext.2984 delphine.eberle@ucsfmedctr.org Vous voulez prot?ger votre num?ro de CB ? Utilisez Internet Explorer 8 _________________________________________________________________ Installez gratuitement les nouvelles Emoch'ticones ! http://www.ilovemessenger.fr/emoticones/telecharger-emoticones-emochticones.aspx From louise.renton <@t> gmail.com Wed Jun 2 02:15:01 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Jun 2 02:15:06 2010 Subject: [Histonet] Need help In-Reply-To: <9BF995BC0E47744E9673A41486E24EE2268C6A54E6@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE2268C6A54E6@MERCERMAIL.MercerU.local> Message-ID: Shirley go to this site for more information on supply, plug type etc http://www.kropla.com/electric2.htm On Tue, Jun 1, 2010 at 6:52 PM, Shirley A. Powell wrote: > Hi Histonetters, > > One of my pathologists is going on a mission to Haiti in July. She is > taking battery powered microscope to use. She is not sure what type of > power is available. All the manual says about the battery option is "the > LED configuration is operational with an in-built re-chargeable battery and > charging circuit. The battery will be charged with a direct input power > supply of 110-240V AC 50Hz/g0Hz which ensures continuous operation under > fluctuating voltages." Greek to me. Has anyone been down since the > earthquake using such battery powered scope? Know of power limitations? If > so, got any tips, clues, pieces of wisdom? I know there are experts out > there who know. > > Thanks, > > > Shirley A. Powell, HT(ASCP)HTL, QIHC > Technical Director > Histology Curricular Support Laboratory > Mercer University School of Medicine > 1550 College Street > Macon, GA 31207 > 478-301-2374 Lab > 478-301-5489 Fax > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From HornHV <@t> archildrens.org Wed Jun 2 08:06:38 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Jun 2 08:07:38 2010 Subject: [Histonet] Review of outside Path In-Reply-To: References: <4C053052.7400.0077.1@harthosp.org>, <370066.57519.qm@web65701.mail.ac4.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83885@EMAIL.archildrens.org> We do not have a policy for this. Our clinicians do often ask our pathologists to review outside slides from previous institutions on patients who are being treated at our facility. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: Tuesday, June 01, 2010 7:58 PM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; rcartun@harthosp.org Subject: RE: [Histonet] Review of outside Path If I am not mistaken, at a hospital I used to work, I remember reviewing a policy prior to our CAP inspection that states patients for surgery, chemotherapy and radiation therapy prior to treatment at this hospital where the diagnosis was made at another institution, slides are requested and reviewed by our Pathologists prior to treatment. I will have to follow-up with the laboratory director and find out if it is a department policy or a hospital policy. Godfrey > Date: Tue, 1 Jun 2010 13:42:36 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; Rcartun@harthosp.org > Subject: Re: [Histonet] Review of outside Path > CC: > > We did not have such a policy. Patient's slides were reviewed only as a consultation sent by their attending pathologists. > Ren? J. > > --- On Tue, 6/1/10, Richard Cartun wrote: > > > From: Richard Cartun > Subject: [Histonet] Review of outside Path > To: "Histonet" > Date: Tuesday, June 1, 2010, 4:07 PM > > > For those of you working in a hospital pathology laboratory, do you have a policy requiring review of outside pathology slides from patients diagnosed with cancer before that patient has surgery, chemotherapy, or radiation therapy at your institution? If so, is it a hospital or departmental policy? Thank you. > > Richard > > Richard W. Cartun, Ph.D. > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From sbreeden <@t> nmda.nmsu.edu Wed Jun 2 10:33:45 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jun 2 10:34:34 2010 Subject: [Histonet] Chlamydia Stain? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47116@nmdamailsvr.nmda.ad.nmsu.edu> Is there a stain for Chlamydia in tissue? My pathologist knows of Gimenez (Jimenez?) for smears but I can't find one for tissues. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 595-841-2576 From TMcNemar <@t> lmhealth.org Wed Jun 2 11:06:27 2010 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jun 2 11:06:41 2010 Subject: [Histonet] Histology position wanted... Message-ID: Hello All, I am posting this for an employee who is relocating to the Chattanooga, TN area in late July or early August. She is a certified HT with 11 years of experience and also holds an MLT certification (both ASCP). She will make someone a wonderful tech and a reliable employee. I am sooooo sad to be losing her. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4061. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From sbreeden <@t> nmda.nmsu.edu Wed Jun 2 11:19:29 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jun 2 11:19:44 2010 Subject: [Histonet] Chlamydia Solution Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47117@nmdamailsvr.nmda.ad.nmsu.edu> After having received several messages (thanks to all of you!), we believe what he wants is an everyday Giemsa. We're going to fly that one past him. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 From rjr6 <@t> psu.edu Wed Jun 2 12:52:09 2010 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Wed Jun 2 12:52:33 2010 Subject: [Histonet] RE: Chlamydia Stain? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47116@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E47116@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: We use the Pierce Van der Kamp Modified Gimenez stain for Chlamydia. I can send it to you if you like. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, June 02, 2010 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chlamydia Stain? Is there a stain for Chlamydia in tissue? My pathologist knows of Gimenez (Jimenez?) for smears but I can't find one for tissues. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 595-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From misbaked <@t> hotmail.com Wed Jun 2 13:41:08 2010 From: misbaked <@t> hotmail.com (MICHELLE BAKER) Date: Wed Jun 2 13:45:56 2010 Subject: [Histonet] use of PGP 9.5 Message-ID: Hi - Does anyone have a procedure using antibody pgp 9.5 for epidermal peripheral nerve fiber count? Missy Baker _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3 From rsrichmond <@t> gmail.com Wed Jun 2 15:19:07 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jun 2 15:19:10 2010 Subject: [Histonet] Re: Review of outside Path Message-ID: Dr. Richard Cartun in Hartford CT asks: >>For those of you working in a hospital pathology laboratory, do you have a policy requiring review of outside pathology slides from patients diagnosed with cancer before that patient has surgery, chemotherapy, or radiation therapy at your institution? If so, is it a hospital or departmental policy?<< Academic institutions do this, but I have never seen this done in private hospitals (maybe 50 of them), most of which do not even have a procedure for accessioning and reporting outside slides. I think such review should be a regulatory requirement, though it would be a burdensome one. At the very least, surgical pathology reports should be reviewed, particularly since the surgeon's office usually has them and can fax them. - I've seen too many errors made because this review wasn't done. - Recent advances in telepathology should make such review a lot more practical. Bob Richmond Samurai Pathologist Knoxville TN From stan <@t> ads4hr.com Wed Jun 2 15:21:06 2010 From: stan <@t> ads4hr.com (Stan Lasater) Date: Wed Jun 2 15:20:25 2010 Subject: [Histonet] Job opening in Port Charlotte, Florida (SW Florida) Message-ID: <2F79E295A6C34D629BA8C6471136D279@ACCA97IOOLUJF4> >From its beginnings in 1962 as a small, one-story community hospital, Peace River Regional Medical Center has since grown into a 219-bed Joint Commission Accredited full service hospital that is appropriately known as: Your Hospital for Life. To continue our vision of becoming a regional health care delivery system, providing a continuum of services, we are seeking an experienced HISTOTECHNOLOGIST for the following role: HISTOLOGY TECHNOLOGIST EXPERIENCE: Minimum: One year training in a Histology program. Preferred: 1 to 2 years as a Histotechnologist . The ability to gross path in accordance with CLIA 88 required. EDUCATION: Associate degree with a chemical, physical or biological science as a major and completion of an accredited Histotechnologist training program. Minimum: Associate degree and national license or equivalent. Preferred: Licensed in Florida as a Histotechnologist. LICENSURE: National license and/or Florida license. This position can perform laboratory procedure in the areas of licensure complying with State & Federal regulations. Supervision and review of testing and results will comply with State & Federal regulations. Minimum: Florida Histotechnologist, HT/HLT. In addition to the rewarding opportunity, Peace River Regional Medical Center offers competitive compensation and benefits as well as a supportive team atmosphere. Our location offers the best in "Florida lifestyle-- Situated minutes from the white, sandy beaches along Florida's west coast, there are activities to suit everyone; world-class dining, shopping, sailing, golfing and more! In addition, our community is a short commute to many of Florida's famous attractions, Miami, Orlando Theme Parks, Major league sporting events, arts and cultural events. If you are seeking a relaxing place to call home, while enjoying the many amenities that only Florida can offer, we invite you to explore it all at Peace River Regional Medical Center. Qualified candidates are encouraged to learn more and apply online at www.peaceriverregional.com . From mpowers <@t> dpspa.com Wed Jun 2 18:21:16 2010 From: mpowers <@t> dpspa.com (Marian Powers) Date: Wed Jun 2 18:21:26 2010 Subject: [Histonet] Lymes IHC Message-ID: Hi: Does anyone know if the antibody Borrelia burgdorferi cross reacts with any other organism besides other spirochetes? Thanks, Marian -- Marian L. Powers, BSOM, HT(ASCP) Manager, Technical Operations Doctors Pathology Services 1253 College Park Drive Dover, DE 19904 302-677-0000-office From gguerzon <@t> live.com Wed Jun 2 21:26:10 2010 From: gguerzon <@t> live.com (Godfrey Guerzon) Date: Wed Jun 2 21:26:15 2010 Subject: [Histonet] Re: Review of outside Path In-Reply-To: References: Message-ID: I have followed up with my former employer (hospital) and they have a policy which the medical director was kind enough to send me a copy. The policy requires that any cancer patient to be treated at this hospital where the cancer diagnosis was not made by their own hospital pathologists - the clinician treating the patient must request the slides from the outside institution(s) where the cancer diagnosis was originally made to be reviewed by the hospital pathologist(s) before treatment is initiated at the hospital. Godfrey > Date: Wed, 2 Jun 2010 16:19:07 -0400 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Review of outside Path > > Dr. Richard Cartun in Hartford CT asks: > > >>For those of you working in a hospital pathology laboratory, do you have a policy requiring review of outside pathology slides from patients diagnosed with cancer before that patient has surgery, chemotherapy, or radiation therapy at your institution? If so, is it a hospital or departmental policy?<< > > Academic institutions do this, but I have never seen this done in > private hospitals (maybe 50 of them), most of which do not even have a > procedure for accessioning and reporting outside slides. I think such > review should be a regulatory requirement, though it would be a > burdensome one. At the very least, surgical pathology reports should > be reviewed, particularly since the surgeon's office usually has them > and can fax them. - I've seen too many errors made because this review > wasn't done. - Recent advances in telepathology should make such > review a lot more practical. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1 From gmaldonado <@t> biovantra.com Thu Jun 3 08:24:15 2010 From: gmaldonado <@t> biovantra.com (Gustavo Maldonado_BV) Date: Thu Jun 3 08:22:19 2010 Subject: [Histonet] Artisan Dako Automated Stainers Message-ID: <896E0BA99CF43249BB5747EB79832D65081E5702@MAIL.vantagehealth.org> I have 2 Artisan automated special stainers for sale. They have only been used for 3 runs each. If anyone is interested please contact me. Gus Maldonado B.S HTL(ASCP) From mpxdjones <@t> yahoo.com Thu Jun 3 08:50:55 2010 From: mpxdjones <@t> yahoo.com (Melissa Jones) Date: Thu Jun 3 08:50:58 2010 Subject: [Histonet] (no subject) Message-ID: <695534.40552.qm@web38508.mail.mud.yahoo.com> Hello everyone, ? I have?just relocated to Houston. I'm searching for a part-time position as a HT.? I have thirteen years of experience as a Sr Histotech.? If anyone knows of any opening positions in the Houston area?please let me know. Thanks, ? Paul Jones HT (ASCP) ? ? ? ? ? ? From gu.lang <@t> gmx.at Thu Jun 3 10:47:32 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jun 3 10:47:49 2010 Subject: [Histonet] searching j-chain antibody for human ffpe Message-ID: Hi! I hope someone can tell me a source of j-chain antibody for FFPE-tissue. Thanks Gudrun Lang From Bill.Tench <@t> pph.org Thu Jun 3 12:49:45 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Thu Jun 3 12:50:00 2010 Subject: [Histonet] outside review. Message-ID: <2820431BF953BB4DA3E9E1A5882265FD0286317D@MAIL1.pph.local> I thought I sent this yesterday, but it didn't address properly. I guess things are different in my part of the world in terms of the frequency of this activity compared with others. Please see the rest. The policy/procedure requiring review of outside slides before definitive treatment (including surgery, radiation therapy, or chemotherapy) in another institution is a fairly common but frequently unenforced policy in many hospitals other than academic institutions. The policy may reside in the Pathology Dept, Surgery Dept, or more frequently (and safely) as a standard Hospital policy. Typically, enforceability is improved if the policy is a Hospital policy. There is an ever growing body of evidence that this policy benefits the patient, the hospital, the clinician, and the pathologist. Compliance is always an issue, especially outside of academic centers, who, out of a belief that the community pathologists are prone to mistakes that academic centers never make, insist on review before treatment. "Everyone's benefit" is always a nice "carrot," but unfortunately, without a "stick," compliance may be a challenge. For the surgeons, at least, the risk of cancellation of the case if a TIMELY request for outside review has not been made serves as a useful "stick," the application of which should be applied judiciously (and perhaps with a flack jacket). Radiation and Chemo cases require either: 1) cooperation of these departments or 2) external review of all cases treated in those departments to determine whether or not case review has occurred. The goal is to, as best as possible, confirm the diagnosis as well as other aspects that may have an impact on the appropriate therapy, and to do so without significant delay in the patient's care. Anything that makes the process easier is a good idea. In my system, the responsibility of the treating physician is to fax to the pathology dept a copy of the report when they have decided to proceed with additional treatment (surgery, rads, etc), and the pathology dept does all the work associated with requesting the slides and insuring a timely review. We advise (and continuously train) the outside office staff that they must provide us this information ASAP because some outside institutions are woefully slow in sending the material to us. (For requests to us from other institutions, we typically send the material to the requesting institution within 24 hours if we don't have to prepare recuts. We re-review all material going elsewhere, and we only send original slides if we have multiple representative levels in our file or we have very limited material-like FNA's, otherwise we recut the case). Dr Bill Tench Assoc Dir. Lab Services Chief Cytology Palomar Medical Center 555 E. Valley Parkway Escondido, Ca 92025 Voice: 760-739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From rcharles <@t> state.pa.us Thu Jun 3 13:42:07 2010 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Thu Jun 3 13:42:15 2010 Subject: [Histonet] Laryngotracheitis Message-ID: <3809C163DC1DA54AA534B3C7794D07B65A8F32BAB1@ENHBGMBX01.PA.LCL> Hello, Is there someone in the veterinary field doing IHC or FA (ffpe or fresh tissue) for infectious laryngotracheitis? I'm searching for a protocol and an antibody. Thanks so much Roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 From raj <@t> bluemarble.net Thu Jun 3 15:20:37 2010 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Thu Jun 3 15:20:53 2010 Subject: [Histonet] Paraplast Message-ID: What paraffin is everyone using. We have been a Paraplast user made by McCormick. We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress. I have tried the a different angle at 3 with no difference. I would appreciate some help from all of you. Thanks Becky From jbaez <@t> interscopepath.com Thu Jun 3 15:52:51 2010 From: jbaez <@t> interscopepath.com (Baez, Janet) Date: Thu Jun 3 15:56:04 2010 Subject: [Histonet] Paraplast Message-ID: <9E956D8FEB06C2408B08AC16498325E90F83DF@scopemx1.interscope.com> We use Richard Allan Scientific Paraffin 3 (item #8335) works great for embedding and infiltrating. Janet Interscope Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Johnson Sent: Thursday, June 03, 2010 1:21 PM To: histonet Subject: [Histonet] Paraplast What paraffin is everyone using. We have been a Paraplast user made by McCormick. We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress. I have tried the a different angle at 3 with no difference. I would appreciate some help from all of you. Thanks Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Adam.T.Anthony <@t> kp.org Thu Jun 3 18:44:04 2010 From: Adam.T.Anthony <@t> kp.org (Adam.T.Anthony@kp.org) Date: Thu Jun 3 18:43:59 2010 Subject: [Histonet] Lab Manager Job Opportunity in San Francisco Message-ID: A position is opening up in July as Lab Manager of the Pathology Department at the Kaiser Permanente San Francisco Medical Center. This is your chance to be part of an exciting department in a wonderful organization. Generous compensation package with great benefits await qualified applicants. Interested candidates should email me for more details (please include a recent resume). Regards, Adam Anthony Manager, Pathology & Regional Immunohistochemistry Kaiser Permanente Medical Center 350 St. Joseph's St. San Francisco, CA 94115 email: adam.t.anthony@kp.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From GaleL <@t> unionhospital.org Fri Jun 4 05:46:18 2010 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Fri Jun 4 05:46:25 2010 Subject: [Histonet] coverslippers Message-ID: We are currently evaluating coverslippers and I'd appreciate any info/opinions on the following: Hacker HCM 6000 TBS SHUR/Mount Thermo Scientific (Shandon) ClearVue Tissue-Tek Glas g2 Leica CV5030 We will be looking at stainers within a couple of years so this is something we need to consider also....... Thanks, Gale Gale Limron Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext. 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From HornHV <@t> archildrens.org Fri Jun 4 06:53:10 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Jun 4 06:53:14 2010 Subject: [Histonet] coverslippers In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83896@EMAIL.archildrens.org> We love our CV5030 glass coverslipper. It integrates with their stainer for a seamless flow. It's so nice to put the slides on to stain and have them coverslipped when they come off. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Friday, June 04, 2010 5:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslippers We are currently evaluating coverslippers and I'd appreciate any info/opinions on the following: Hacker HCM 6000 TBS SHUR/Mount Thermo Scientific (Shandon) ClearVue Tissue-Tek Glas g2 Leica CV5030 We will be looking at stainers within a couple of years so this is something we need to consider also....... Thanks, Gale Gale Limron Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext. 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From HornHV <@t> archildrens.org Fri Jun 4 06:55:39 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Jun 4 06:55:47 2010 Subject: [Histonet] INI-1 Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83897@EMAIL.archildrens.org> We are having problems with this immunostain. If your lab is successfully using this antibody will you please help me out? Where do you purchase your antibody? Is it RTU? What type of antigen retrieval? Incubation time? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From DKBoyd <@t> chs.net Fri Jun 4 08:08:43 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Jun 4 08:08:49 2010 Subject: [Histonet] Paraplast In-Reply-To: Message-ID: We use Richard Allen, Type 6 for both impregnation and embedding. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Rebecca Johnson" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/03/2010 04:42 PM To "histonet" cc Subject [Histonet] Paraplast What paraffin is everyone using. We have been a Paraplast user made by McCormick. We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress. I have tried the a different angle at 3 with no difference. I would appreciate some help from all of you. Thanks Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From andreas.kappeler <@t> pathology.unibe.ch Fri Jun 4 08:28:00 2010 From: andreas.kappeler <@t> pathology.unibe.ch (Kappeler, Andreas (PATHOLOGY)) Date: Fri Jun 4 08:28:08 2010 Subject: [Histonet] AW: INI-1 In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83897@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83897@EMAIL.archildrens.org> Message-ID: <4EAFACC375BF974C8A57D33444CEE288049AEFDD64@AAI-MBX3.campus.unibe.ch> Hi Hazel We use INI-1, clone 25/BAF47, BD Transduction 612110. Working conc. is 2.5 mg Ig/l (1:100), HIER at high pH with a somewhat strange buffer (800 mM urea, 100 mM Tris, pH 9.5), but you may try EDTA-based HIER solutions, pH 8.8-9.5. Incubation at RT, 60 min. Visualization with polymer-based HRP system/DAB. Hope this helps. Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland P.S. when asking about advice on antibodies, try to be as specific as possible: clone, Ig-conc and/or dilution, retrieval protocols tried, visualization systems tried. Helps o narrow down possilbe problems. ________________________________________ Von: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] im Auftrag von Horn, Hazel V [HornHV@archildrens.org] Gesendet: Freitag, 4. Juni 2010 13:55 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] INI-1 We are having problems with this immunostain. If your lab is successfully using this antibody will you please help me out? Where do you purchase your antibody? Is it RTU? What type of antigen retrieval? Incubation time? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Jun 4 08:36:16 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Jun 4 08:37:37 2010 Subject: [Histonet] RE: INI-1 In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83897@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83897@EMAIL.archildrens.org> Message-ID: We have been using an INI-1 from Cell Marque. With a 1/50 dilution and a low pH heat retrieval. Although I accidently had a slide go through the higher pH heat retrieval and I didn't see a big difference in the staining. I use the Dako system so it was a 30 minute incubation with a linker added. Hope that helps. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V [HornHV@archildrens.org] Sent: Friday, June 04, 2010 7:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] INI-1 We are having problems with this immunostain. If your lab is successfully using this antibody will you please help me out? Where do you purchase your antibody? Is it RTU? What type of antigen retrieval? Incubation time? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMcCormick <@t> schosp.org Fri Jun 4 10:03:49 2010 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Fri Jun 4 10:03:59 2010 Subject: [Histonet] Unsubscribe. Message-ID: <0CA58D7DD405854899D129A03276B133443D48970E@EXCHCCRMB.schosp.org> Please unsubscribe my email from your list. Thank you, James B. McCormick, M.D. ________________________________ *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From Albert.Santiago <@t> uphs.upenn.edu Fri Jun 4 10:30:43 2010 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Fri Jun 4 10:30:57 2010 Subject: [Histonet] H&E Stainer Validations Message-ID: Hello fellow Histonetters, I'm going to Demo a new H&E stainer and I was wondering if anyone knows if there's a standard H&E Validation protocol or if it just varies from lab to lab? Thanks for your help.... :-) Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6759/6539-office 215-520-3797-BB 215-662-7520-fax We will be relocating, as of 6/14/2010 our lab will be located at 3020 Market St. Ste. 201 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From talulahgosh <@t> gmail.com Fri Jun 4 10:54:52 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jun 4 10:54:58 2010 Subject: [Histonet] Unsubscribe. In-Reply-To: <0CA58D7DD405854899D129A03276B133443D48970E@EXCHCCRMB.schosp.org> References: <0CA58D7DD405854899D129A03276B133443D48970E@EXCHCCRMB.schosp.org> Message-ID: No. Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Fri, Jun 4, 2010 at 11:03 AM, McCormick, James wrote: > Please unsubscribe my email from your list. > > Thank you, > James B. McCormick, M.D. > > ________________________________ > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged and > confidential. If the reader of this message is not the intended recipient, > please notify the sender immediately by replying to this message and then > delete it from your system. Any review, dissemination, distribution, or > reproduction of this message by unintended recipients is strictly prohibited > and may be subject to legal restriction. > > > Thank you for your cooperation. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DKnutson <@t> primecare.org Fri Jun 4 12:39:44 2010 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Fri Jun 4 12:39:49 2010 Subject: [Histonet] Control Blocks Message-ID: <1E0E2B14C709174B8AC2BE0AE7F768338FE967C875@EXCHANGE2K7.staprimecare.org> Hello, Does anyone out there have positive Pneumocystis control blocks that they may want to trade with? We have some positive GRAM control blocks that we could exchange. Please let me know. Thank you so much! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From Karen.Kay <@t> albertahealthservices.ca Fri Jun 4 12:43:05 2010 From: Karen.Kay <@t> albertahealthservices.ca (Karen Kay) Date: Fri Jun 4 12:43:21 2010 Subject: [Histonet] PARAPLAST QUALITY In-Reply-To: <20100604172314.4D31922AEBA@scutcheon.calgaryhealthregion.ca> References: <20100604172314.4D31922AEBA@scutcheon.calgaryhealthregion.ca> Message-ID: Good Morning, Our Paraplast has also been produced by McCormick and to this point have not seen any issues. However it is my understanding that Leica(Surgipath) has taken over as supplier and producer of the Paraplast (trademark). This information was relayed to us by our Surgipath representative quite a few months ago. This past week, I received a phone call from a different Lab supplier indicating to me that there have been issues cropping up with the Paraplast from the new manufacturer. I did do a search on the Histonet and have not found messages to that effect. It would be interesting and proactive to find out if there indeed have been issues cropping up recently with the Paraplast. Thanks Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital-South Zone West - Alberta Health Services Lethbridge, Alberta, Canada karen.kay@albertahealthservices.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: June 4, 2010 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. outside review. (Tench, Bill) 2. Laryngotracheitis (Charles, Roger) 3. Paraplast (Rebecca Johnson) 4. RE: Paraplast (Baez, Janet) 5. Lab Manager Job Opportunity in San Francisco (Adam.T.Anthony@kp.org) 6. coverslippers (Gale Limron) 7. RE: coverslippers (Horn, Hazel V) 8. INI-1 (Horn, Hazel V) 9. Re: Paraplast (DKBoyd@chs.net) 10. AW: INI-1 (Kappeler, Andreas (PATHOLOGY)) 11. RE: INI-1 (McMahon, Loralee A) 12. Unsubscribe. (McCormick, James) 13. H&E Stainer Validations (Santiago, Albert) 14. Re: Unsubscribe. (Emily Sours) ---------------------------------------------------------------------- Message: 1 Date: Thu, 3 Jun 2010 10:49:45 -0700 From: "Tench, Bill" Subject: [Histonet] outside review. To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD0286317D@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii I thought I sent this yesterday, but it didn't address properly. I guess things are different in my part of the world in terms of the frequency of this activity compared with others. Please see the rest. The policy/procedure requiring review of outside slides before definitive treatment (including surgery, radiation therapy, or chemotherapy) in another institution is a fairly common but frequently unenforced policy in many hospitals other than academic institutions. The policy may reside in the Pathology Dept, Surgery Dept, or more frequently (and safely) as a standard Hospital policy. Typically, enforceability is improved if the policy is a Hospital policy. There is an ever growing body of evidence that this policy benefits the patient, the hospital, the clinician, and the pathologist. Compliance is always an issue, especially outside of academic centers, who, out of a belief that the community pathologists are prone to mistakes that academic centers never make, insist on review before treatment. "Everyone's benefit" is always a nice "carrot," but unfortunately, without a "stick," compliance may be a challenge. For the surgeons, at least, the risk of cancellation of the case if a TIMELY request for outside review has not been made serves as a useful "stick," the application of which should be applied judiciously (and perhaps with a flack jacket). Radiation and Chemo cases require either: 1) cooperation of these departments or 2) external review of all cases treated in those departments to determine whether or not case review has occurred. The goal is to, as best as possible, confirm the diagnosis as well as other aspects that may have an impact on the appropriate therapy, and to do so without significant delay in the patient's care. Anything that makes the process easier is a good idea. In my system, the responsibility of the treating physician is to fax to the pathology dept a copy of the report when they have decided to proceed with additional treatment (surgery, rads, etc), and the pathology dept does all the work associated with requesting the slides and insuring a timely review. We advise (and continuously train) the outside office staff that they must provide us this information ASAP because some outside institutions are woefully slow in sending the material to us. (For requests to us from other institutions, we typically send the material to the requesting institution within 24 hours if we don't have to prepare recuts. We re-review all material going elsewhere, and we only send original slides if we have multiple representative levels in our file or we have very limited material-like FNA's, otherwise we recut the case). Dr Bill Tench Assoc Dir. Lab Services Chief Cytology Palomar Medical Center 555 E. Valley Parkway Escondido, Ca 92025 Voice: 760-739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 2 Date: Thu, 3 Jun 2010 14:42:07 -0400 From: "Charles, Roger" Subject: [Histonet] Laryngotracheitis To: "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: <3809C163DC1DA54AA534B3C7794D07B65A8F32BAB1@ENHBGMBX01.PA.LCL> Content-Type: text/plain; charset="us-ascii" Hello, Is there someone in the veterinary field doing IHC or FA (ffpe or fresh tissue) for infectious laryngotracheitis? I'm searching for a protocol and an antibody. Thanks so much Roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 ------------------------------ Message: 3 Date: Thu, 3 Jun 2010 15:20:37 -0500 From: "Rebecca Johnson" Subject: [Histonet] Paraplast To: "histonet" Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original What paraffin is everyone using. We have been a Paraplast user made by McCormick. We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress. I have tried the a different angle at 3 with no difference. I would appreciate some help from all of you. Thanks Becky ------------------------------ Message: 4 Date: Thu, 3 Jun 2010 13:52:51 -0700 From: "Baez, Janet" Subject: RE: [Histonet] Paraplast To: "Rebecca Johnson" , "histonet" Message-ID: <9E956D8FEB06C2408B08AC16498325E90F83DF@scopemx1.interscope.com> Content-Type: text/plain; charset="us-ascii" We use Richard Allan Scientific Paraffin 3 (item #8335) works great for embedding and infiltrating. Janet Interscope Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Johnson Sent: Thursday, June 03, 2010 1:21 PM To: histonet Subject: [Histonet] Paraplast What paraffin is everyone using. We have been a Paraplast user made by McCormick. We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress. I have tried the a different angle at 3 with no difference. I would appreciate some help from all of you. Thanks Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 3 Jun 2010 16:44:04 -0700 From: Adam.T.Anthony@kp.org Subject: [Histonet] Lab Manager Job Opportunity in San Francisco To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" A position is opening up in July as Lab Manager of the Pathology Department at the Kaiser Permanente San Francisco Medical Center. This is your chance to be part of an exciting department in a wonderful organization. Generous compensation package with great benefits await qualified applicants. Interested candidates should email me for more details (please include a recent resume). Regards, Adam Anthony Manager, Pathology & Regional Immunohistochemistry Kaiser Permanente Medical Center 350 St. Joseph's St. San Francisco, CA 94115 email: adam.t.anthony@kp.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. ------------------------------ Message: 6 Date: Fri, 4 Jun 2010 06:46:18 -0400 From: Gale Limron Subject: [Histonet] coverslippers To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We are currently evaluating coverslippers and I'd appreciate any info/opinions on the following: Hacker HCM 6000 TBS SHUR/Mount Thermo Scientific (Shandon) ClearVue Tissue-Tek Glas g2 Leica CV5030 We will be looking at stainers within a couple of years so this is something we need to consider also....... Thanks, Gale Gale Limron Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext. 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. ------------------------------ Message: 7 Date: Fri, 4 Jun 2010 06:53:10 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] coverslippers To: "Gale Limron" , Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83896@EMAIL.archildrens.org> Content-Type: text/plain; charset="US-ASCII" We love our CV5030 glass coverslipper. It integrates with their stainer for a seamless flow. It's so nice to put the slides on to stain and have them coverslipped when they come off. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Friday, June 04, 2010 5:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslippers We are currently evaluating coverslippers and I'd appreciate any info/opinions on the following: Hacker HCM 6000 TBS SHUR/Mount Thermo Scientific (Shandon) ClearVue Tissue-Tek Glas g2 Leica CV5030 We will be looking at stainers within a couple of years so this is something we need to consider also....... Thanks, Gale Gale Limron Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext. 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 8 Date: Fri, 4 Jun 2010 06:55:39 -0500 From: "Horn, Hazel V" Subject: [Histonet] INI-1 To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83897@EMAIL.archildrens.org> Content-Type: text/plain; charset="ISO-8859-1" We are having problems with this immunostain. If your lab is successfully using this antibody will you please help me out? Where do you purchase your antibody? Is it RTU? What type of antigen retrieval? Incubation time? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 9 Date: Fri, 4 Jun 2010 09:08:43 -0400 From: DKBoyd@chs.net Subject: Re: [Histonet] Paraplast To: "Rebecca Johnson" Cc: histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We use Richard Allen, Type 6 for both impregnation and embedding. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Rebecca Johnson" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/03/2010 04:42 PM To "histonet" cc Subject [Histonet] Paraplast What paraffin is everyone using. We have been a Paraplast user made by McCormick. We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress. I have tried the a different angle at 3 with no difference. I would appreciate some help from all of you. Thanks Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 10 Date: Fri, 4 Jun 2010 15:28:00 +0200 From: "Kappeler, Andreas (PATHOLOGY)" Subject: [Histonet] AW: INI-1 To: "Horn, Hazel V" , "histonet@lists.utsouthwestern.edu" Message-ID: <4EAFACC375BF974C8A57D33444CEE288049AEFDD64@AAI-MBX3.campus.unibe.ch> Content-Type: text/plain; charset="us-ascii" Hi Hazel We use INI-1, clone 25/BAF47, BD Transduction 612110. Working conc. is 2.5 mg Ig/l (1:100), HIER at high pH with a somewhat strange buffer (800 mM urea, 100 mM Tris, pH 9.5), but you may try EDTA-based HIER solutions, pH 8.8-9.5. Incubation at RT, 60 min. Visualization with polymer-based HRP system/DAB. Hope this helps. Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland P.S. when asking about advice on antibodies, try to be as specific as possible: clone, Ig-conc and/or dilution, retrieval protocols tried, visualization systems tried. Helps o narrow down possilbe problems. ________________________________________ Von: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] im Auftrag von Horn, Hazel V [HornHV@archildrens.org] Gesendet: Freitag, 4. Juni 2010 13:55 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] INI-1 We are having problems with this immunostain. If your lab is successfully using this antibody will you please help me out? Where do you purchase your antibody? Is it RTU? What type of antigen retrieval? Incubation time? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 4 Jun 2010 09:36:16 -0400 From: "McMahon, Loralee A" Subject: [Histonet] RE: INI-1 To: "Horn, Hazel V" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have been using an INI-1 from Cell Marque. With a 1/50 dilution and a low pH heat retrieval. Although I accidently had a slide go through the higher pH heat retrieval and I didn't see a big difference in the staining. I use the Dako system so it was a 30 minute incubation with a linker added. Hope that helps. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V [HornHV@archildrens.org] Sent: Friday, June 04, 2010 7:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] INI-1 We are having problems with this immunostain. If your lab is successfully using this antibody will you please help me out? Where do you purchase your antibody? Is it RTU? What type of antigen retrieval? Incubation time? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 4 Jun 2010 10:03:49 -0500 From: "McCormick, James" Subject: [Histonet] Unsubscribe. To: "Histonet@lists.utsouthwestern.edu" Message-ID: <0CA58D7DD405854899D129A03276B133443D48970E@EXCHCCRMB.schosp.org> Content-Type: text/plain; charset="us-ascii" Please unsubscribe my email from your list. Thank you, James B. McCormick, M.D. ________________________________ *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. ------------------------------ Message: 13 Date: Fri, 4 Jun 2010 11:30:43 -0400 From: "Santiago, Albert" Subject: [Histonet] H&E Stainer Validations To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello fellow Histonetters, I'm going to Demo a new H&E stainer and I was wondering if anyone knows if there's a standard H&E Validation protocol or if it just varies from lab to lab? Thanks for your help.... :-) Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6759/6539-office 215-520-3797-BB 215-662-7520-fax We will be relocating, as of 6/14/2010 our lab will be located at 3020 Market St. Ste. 201 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 14 Date: Fri, 4 Jun 2010 11:54:52 -0400 From: Emily Sours Subject: Re: [Histonet] Unsubscribe. To: "McCormick, James" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 No. Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Fri, Jun 4, 2010 at 11:03 AM, McCormick, James wrote: > Please unsubscribe my email from your list. > > Thank you, > James B. McCormick, M.D. > > ________________________________ > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged and > confidential. If the reader of this message is not the intended recipient, > please notify the sender immediately by replying to this message and then > delete it from your system. Any review, dissemination, distribution, or > reproduction of this message by unintended recipients is strictly prohibited > and may be subject to legal restriction. > > > Thank you for your cooperation. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 4 *************************************** This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From STACEY.LANGENBERG <@t> UCDENVER.EDU Fri Jun 4 13:17:25 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Fri Jun 4 13:17:40 2010 Subject: [Histonet] PARAPLAST QUALITY In-Reply-To: References: <20100604172314.4D31922AEBA@scutcheon.calgaryhealthregion.ca> Message-ID: <727425792.763577.1275675449430.JavaMail.rim@bda2340.bisx.prod.on.blackberry> We use paraplast Xtra supplied from Leica\Surgipath and have seen no problems whatsoever. Stacey Langenberg Sent via BlackBerry from T-Mobile -----Original Message----- From: Karen Kay Date: Fri, 4 Jun 2010 11:43:05 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] PARAPLAST QUALITY Good Morning, Our Paraplast has also been produced by McCormick and to this point have not seen any issues. However it is my understanding that Leica(Surgipath) has taken over as supplier and producer of the Paraplast (trademark). This information was relayed to us by our Surgipath representative quite a few months ago. This past week, I received a phone call from a different Lab supplier indicating to me that there have been issues cropping up with the Paraplast from the new manufacturer. I did do a search on the Histonet and have not found messages to that effect. It would be interesting and proactive to find out if there indeed have been issues cropping up recently with the Paraplast. Thanks Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital-South Zone West - Alberta Health Services Lethbridge, Alberta, Canada karen.kay@albertahealthservices.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: June 4, 2010 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. outside review. (Tench, Bill) 2. Laryngotracheitis (Charles, Roger) 3. Paraplast (Rebecca Johnson) 4. RE: Paraplast (Baez, Janet) 5. Lab Manager Job Opportunity in San Francisco (Adam.T.Anthony@kp.org) 6. coverslippers (Gale Limron) 7. RE: coverslippers (Horn, Hazel V) 8. INI-1 (Horn, Hazel V) 9. Re: Paraplast (DKBoyd@chs.net) 10. AW: INI-1 (Kappeler, Andreas (PATHOLOGY)) 11. RE: INI-1 (McMahon, Loralee A) 12. Unsubscribe. (McCormick, James) 13. H&E Stainer Validations (Santiago, Albert) 14. Re: Unsubscribe. (Emily Sours) ---------------------------------------------------------------------- Message: 1 Date: Thu, 3 Jun 2010 10:49:45 -0700 From: "Tench, Bill" Subject: [Histonet] outside review. To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD0286317D@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii I thought I sent this yesterday, but it didn't address properly. I guess things are different in my part of the world in terms of the frequency of this activity compared with others. Please see the rest. The policy/procedure requiring review of outside slides before definitive treatment (including surgery, radiation therapy, or chemotherapy) in another institution is a fairly common but frequently unenforced policy in many hospitals other than academic institutions. The policy may reside in the Pathology Dept, Surgery Dept, or more frequently (and safely) as a standard Hospital policy. Typically, enforceability is improved if the policy is a Hospital policy. There is an ever growing body of evidence that this policy benefits the patient, the hospital, the clinician, and the pathologist. Compliance is always an issue, especially outside of academic centers, who, out of a belief that the community pathologists are prone to mistakes that academic centers never make, insist on review before treatment. "Everyone's benefit" is always a nice "carrot," but unfortunately, without a "stick," compliance may be a challenge. For the surgeons, at least, the risk of cancellation of the case if a TIMELY request for outside review has not been made serves as a useful "stick," the application of which should be applied judiciously (and perhaps with a flack jacket). Radiation and Chemo cases require either: 1) cooperation of these departments or 2) external review of all cases treated in those departments to determine whether or not case review has occurred. The goal is to, as best as possible, confirm the diagnosis as well as other aspects that may have an impact on the appropriate therapy, and to do so without significant delay in the patient's care. Anything that makes the process easier is a good idea. In my system, the responsibility of the treating physician is to fax to the pathology dept a copy of the report when they have decided to proceed with additional treatment (surgery, rads, etc), and the pathology dept does all the work associated with requesting the slides and insuring a timely review. We advise (and continuously train) the outside office staff that they must provide us this information ASAP because some outside institutions are woefully slow in sending the material to us. (For requests to us from other institutions, we typically send the material to the requesting institution within 24 hours if we don't have to prepare recuts. We re-review all material going elsewhere, and we only send original slides if we have multiple representative levels in our file or we have very limited material-like FNA's, otherwise we recut the case). Dr Bill Tench Assoc Dir. Lab Services Chief Cytology Palomar Medical Center 555 E. Valley Parkway Escondido, Ca 92025 Voice: 760-739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 2 Date: Thu, 3 Jun 2010 14:42:07 -0400 From: "Charles, Roger" Subject: [Histonet] Laryngotracheitis To: "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: <3809C163DC1DA54AA534B3C7794D07B65A8F32BAB1@ENHBGMBX01.PA.LCL> Content-Type: text/plain; charset="us-ascii" Hello, Is there someone in the veterinary field doing IHC or FA (ffpe or fresh tissue) for infectious laryngotracheitis? I'm searching for a protocol and an antibody. Thanks so much Roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 ------------------------------ Message: 3 Date: Thu, 3 Jun 2010 15:20:37 -0500 From: "Rebecca Johnson" Subject: [Histonet] Paraplast To: "histonet" Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original What paraffin is everyone using. We have been a Paraplast user made by McCormick. We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress. I have tried the a different angle at 3 with no difference. I would appreciate some help from all of you. Thanks Becky ------------------------------ Message: 4 Date: Thu, 3 Jun 2010 13:52:51 -0700 From: "Baez, Janet" Subject: RE: [Histonet] Paraplast To: "Rebecca Johnson" , "histonet" Message-ID: <9E956D8FEB06C2408B08AC16498325E90F83DF@scopemx1.interscope.com> Content-Type: text/plain; charset="us-ascii" We use Richard Allan Scientific Paraffin 3 (item #8335) works great for embedding and infiltrating. Janet Interscope Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Johnson Sent: Thursday, June 03, 2010 1:21 PM To: histonet Subject: [Histonet] Paraplast What paraffin is everyone using. We have been a Paraplast user made by McCormick. We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress. I have tried the a different angle at 3 with no difference. I would appreciate some help from all of you. Thanks Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 3 Jun 2010 16:44:04 -0700 From: Adam.T.Anthony@kp.org Subject: [Histonet] Lab Manager Job Opportunity in San Francisco To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" A position is opening up in July as Lab Manager of the Pathology Department at the Kaiser Permanente San Francisco Medical Center. This is your chance to be part of an exciting department in a wonderful organization. Generous compensation package with great benefits await qualified applicants. Interested candidates should email me for more details (please include a recent resume). Regards, Adam Anthony Manager, Pathology & Regional Immunohistochemistry Kaiser Permanente Medical Center 350 St. Joseph's St. San Francisco, CA 94115 email: adam.t.anthony@kp.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. ------------------------------ Message: 6 Date: Fri, 4 Jun 2010 06:46:18 -0400 From: Gale Limron Subject: [Histonet] coverslippers To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We are currently evaluating coverslippers and I'd appreciate any info/opinions on the following: Hacker HCM 6000 TBS SHUR/Mount Thermo Scientific (Shandon) ClearVue Tissue-Tek Glas g2 Leica CV5030 We will be looking at stainers within a couple of years so this is something we need to consider also....... Thanks, Gale Gale Limron Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext. 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. ------------------------------ Message: 7 Date: Fri, 4 Jun 2010 06:53:10 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] coverslippers To: "Gale Limron" , Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83896@EMAIL.archildrens.org> Content-Type: text/plain; charset="US-ASCII" We love our CV5030 glass coverslipper. It integrates with their stainer for a seamless flow. It's so nice to put the slides on to stain and have them coverslipped when they come off. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Friday, June 04, 2010 5:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslippers We are currently evaluating coverslippers and I'd appreciate any info/opinions on the following: Hacker HCM 6000 TBS SHUR/Mount Thermo Scientific (Shandon) ClearVue Tissue-Tek Glas g2 Leica CV5030 We will be looking at stainers within a couple of years so this is something we need to consider also....... Thanks, Gale Gale Limron Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext. 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 8 Date: Fri, 4 Jun 2010 06:55:39 -0500 From: "Horn, Hazel V" Subject: [Histonet] INI-1 To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83897@EMAIL.archildrens.org> Content-Type: text/plain; charset="ISO-8859-1" We are having problems with this immunostain. If your lab is successfully using this antibody will you please help me out? Where do you purchase your antibody? Is it RTU? What type of antigen retrieval? Incubation time? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 9 Date: Fri, 4 Jun 2010 09:08:43 -0400 From: DKBoyd@chs.net Subject: Re: [Histonet] Paraplast To: "Rebecca Johnson" Cc: histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We use Richard Allen, Type 6 for both impregnation and embedding. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Rebecca Johnson" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/03/2010 04:42 PM To "histonet" cc Subject [Histonet] Paraplast What paraffin is everyone using. We have been a Paraplast user made by McCormick. We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress. I have tried the a different angle at 3 with no difference. I would appreciate some help from all of you. Thanks Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 10 Date: Fri, 4 Jun 2010 15:28:00 +0200 From: "Kappeler, Andreas (PATHOLOGY)" Subject: [Histonet] AW: INI-1 To: "Horn, Hazel V" , "histonet@lists.utsouthwestern.edu" Message-ID: <4EAFACC375BF974C8A57D33444CEE288049AEFDD64@AAI-MBX3.campus.unibe.ch> Content-Type: text/plain; charset="us-ascii" Hi Hazel We use INI-1, clone 25/BAF47, BD Transduction 612110. Working conc. is 2.5 mg Ig/l (1:100), HIER at high pH with a somewhat strange buffer (800 mM urea, 100 mM Tris, pH 9.5), but you may try EDTA-based HIER solutions, pH 8.8-9.5. Incubation at RT, 60 min. Visualization with polymer-based HRP system/DAB. Hope this helps. Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland P.S. when asking about advice on antibodies, try to be as specific as possible: clone, Ig-conc and/or dilution, retrieval protocols tried, visualization systems tried. Helps o narrow down possilbe problems. ________________________________________ Von: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] im Auftrag von Horn, Hazel V [HornHV@archildrens.org] Gesendet: Freitag, 4. Juni 2010 13:55 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] INI-1 We are having problems with this immunostain. If your lab is successfully using this antibody will you please help me out? Where do you purchase your antibody? Is it RTU? What type of antigen retrieval? Incubation time? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 4 Jun 2010 09:36:16 -0400 From: "McMahon, Loralee A" Subject: [Histonet] RE: INI-1 To: "Horn, Hazel V" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have been using an INI-1 from Cell Marque. With a 1/50 dilution and a low pH heat retrieval. Although I accidently had a slide go through the higher pH heat retrieval and I didn't see a big difference in the staining. I use the Dako system so it was a 30 minute incubation with a linker added. Hope that helps. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V [HornHV@archildrens.org] Sent: Friday, June 04, 2010 7:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] INI-1 We are having problems with this immunostain. If your lab is successfully using this antibody will you please help me out? Where do you purchase your antibody? Is it RTU? What type of antigen retrieval? Incubation time? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 4 Jun 2010 10:03:49 -0500 From: "McCormick, James" Subject: [Histonet] Unsubscribe. To: "Histonet@lists.utsouthwestern.edu" Message-ID: <0CA58D7DD405854899D129A03276B133443D48970E@EXCHCCRMB.schosp.org> Content-Type: text/plain; charset="us-ascii" Please unsubscribe my email from your list. Thank you, James B. McCormick, M.D. ________________________________ *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. ------------------------------ Message: 13 Date: Fri, 4 Jun 2010 11:30:43 -0400 From: "Santiago, Albert" Subject: [Histonet] H&E Stainer Validations To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello fellow Histonetters, I'm going to Demo a new H&E stainer and I was wondering if anyone knows if there's a standard H&E Validation protocol or if it just varies from lab to lab? Thanks for your help.... :-) Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6759/6539-office 215-520-3797-BB 215-662-7520-fax We will be relocating, as of 6/14/2010 our lab will be located at 3020 Market St. Ste. 201 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 14 Date: Fri, 4 Jun 2010 11:54:52 -0400 From: Emily Sours Subject: Re: [Histonet] Unsubscribe. To: "McCormick, James" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 No. Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Fri, Jun 4, 2010 at 11:03 AM, McCormick, James wrote: > Please unsubscribe my email from your list. > > Thank you, > James B. McCormick, M.D. > >________________________________ > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged and > confidential. If the reader of this message is not the intended recipient, > please notify the sender immediately by replying to this message and then > delete it from your system. Any review, dissemination, distribution, or > reproduction of this message by unintended recipients is strictly prohibited > and may be subject to legal restriction. > > > Thank you for your cooperation. >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 4 *************************************** This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jun 4 15:16:43 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 4 15:16:45 2010 Subject: [Histonet] H&E Stainer Validations In-Reply-To: Message-ID: <123223.53840.qm@web65710.mail.ac4.yahoo.com> Albert: In histology there are only very few standards. That is why CAP has a check list when inspecting histolabs. If you are doing a "validation", get as many different tissue sections as you can. Stain them "as usual" (namely using?the method you are trying to substitute). Stain identical sections with your method or instruyment. Label them just by numbers, and give them to the pathologist for a blind evaluation. They should answer just one questions: is either section within each pair better than the other (telling which one) or are they equally useful for diagnosis. The results analyze with Chi a square test where the "theoretical" frequency is P=0.50. As "simple" as that. Ren? J.? --- On Fri, 6/4/10, Santiago, Albert wrote: From: Santiago, Albert Subject: [Histonet] H&E Stainer Validations To: histonet@lists.utsouthwestern.edu Date: Friday, June 4, 2010, 11:30 AM Hello fellow Histonetters, I'm going to Demo a new H&E stainer and I was wondering if anyone knows if there's a standard H&E Validation protocol or if it just varies from lab to lab? Thanks for your help.... :-) Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6759/6539-office 215-520-3797-BB 215-662-7520-fax We will be relocating, as of 6/14/2010 our lab will be located at 3020 Market St. Ste. 201 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Fri Jun 4 18:01:37 2010 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Jun 4 18:01:46 2010 Subject: [Histonet] I will be out of office beginning the afternoon of 6/3/2010 returning 3/24/2010 Message-ID: I will be out of the office starting 06/03/2010 and will not return until 06/07/2010. .In my absence please ask for Mary Campbell . If this is urgent you can contact me on my cell phone number 858-472-4266. From gayle.callis <@t> bresnan.net Sun Jun 6 12:16:37 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sun Jun 6 12:16:50 2010 Subject: [Histonet] Has Richard Allan Hematoxylin 1 been discontinued by ThermoScientific Message-ID: <000001cb059c$06ce1fc0$146a5f40$@callis@bresnan.net> Has Richard Allan Hematoxylin 1 been discontinued by ThermoScientific, after all they own RA? I am helping put together a supply list for laboratory, and am having trouble finding this on Thermo Scientific website. I did not see it on the Fisher Heathcare site either. Personally, and I hate to complain, but I am finding Thermo's website a nightmare to navigate. I may have to go to VWR and Cardinal who seem to have it, then why not Thermo? Impatiently, Gayle M. Callis HTL/HT/MT(ASCP) From karen <@t> gateslinger.com Sun Jun 6 21:34:32 2010 From: karen <@t> gateslinger.com (Karen Lahti) Date: Sun Jun 6 21:34:02 2010 Subject: [Histonet] CIMP Message-ID: <009a01cb05e9$f618cb50$1a07a8c0@cranium> I am looking for a lab I can reference some slides to for CIMP (CpG Island Methylator Phenotype). Karen D. Lahti, HT(ASCP)QIHC, MLT(AMT) Histology Supervisor Arizona Digestive Health 1300 N. 12th Street #550 Phoenix, AZ 85006 480-236-6559 cell 602-687-7218 office From arsenn <@t> hsh.org Mon Jun 7 08:27:19 2010 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Mon Jun 7 08:27:59 2010 Subject: [Histonet] Oncotech Message-ID: Hello Histonet, Now that Oncotech is out of business, who is everyone sending their extreme resistance assays to? Thanks, Amy, HT Camp Hill, PA Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jun 7 09:13:30 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jun 7 09:13:37 2010 Subject: [Histonet] Lot numbers Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB2668@HPEMX3.HealthPartners.int> Would anyone be willing to share with me how you record lot numbers of all reagents and histology components as they are used? I have a couple of ideas on how to capture this, but, they seem too detailed!! Obviously, our IHC and special stains done on the automated stainer are documented by the equipment, but, looking at all the hand stains, H&E's, processors, etc.,etc!! Thank you for any guidance in this area!! Dorothy Webb, HT Regions Histology Technical Superviosr 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From kgrobert <@t> rci.rutgers.edu Mon Jun 7 09:17:08 2010 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Mon Jun 7 09:17:16 2010 Subject: [Histonet] Has Richard Allan Hematoxylin 1 been discontinued by ThermoScientific In-Reply-To: <000001cb059c$06ce1fc0$146a5f40$@callis@bresnan.net> References: <000001cb059c$06ce1fc0$146a5f40$@callis@bresnan.net> Message-ID: <706dbcc1fc672513529d6e57cbcf48b1.squirrel@webmail.rci.rutgers.edu> Just saw this email now. Here it is: http://www.fishersci.com/wps/portal/PRODUCTDETAIL?prodcutdetail=%27prod%27&productId=5001566&catalogId=29103&matchedCatNo=22050112&pos=7&catCode=HC_SC&endecaSearchQuery=%23store%3DHealthCare%23N%3D0%23rpp%3D15&fromCat=yes&keepSessionSearchOutPut=true&fromSearch=Y&searchKey=richards||hematoxylin||Allan||Richard&highlightProductsItemsFlag=Y Hope this helps, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 > Has Richard Allan Hematoxylin 1 been discontinued by ThermoScientific, > after > all they own RA? I am helping put together a supply list for > laboratory, > and am having trouble finding this on Thermo Scientific website. I did > not > see it on the Fisher Heathcare site either. Personally, and I hate to > complain, but I am finding Thermo's website a nightmare to navigate. I > may > have to go to VWR and Cardinal who seem to have it, then why not Thermo? > > > > Impatiently, > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cbarone <@t> NEMOURS.ORG Mon Jun 7 09:45:58 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon Jun 7 09:46:05 2010 Subject: [Histonet] Region II meeting in NJ - will take wak-ins Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7D79@wlmmsx01.nemours.org> Histonetters... Good news for you..if you are one of those people who are always late or procrastinating....! Pennsylvania's late reminder ...is your benefit! Flyers regarding the Region II Symposium in NJ, were just sent to eastern PA facilitites last week (due to the temporary inactive state of their society). Therefore, we have decided to waive all late fees for registration, to walk-ins at the Region II Symposium...for Pennsylvanians and everyone! So to those of you in PA and everywhere....it is not too late to come to the Region II Symposium June, 10.11.12 - walk-in... and register...with no added fees. Clarion Hotel and Convention Center, Atlantic City West, NJ We still have workshops and seminars that are open: 25 speakers are presenting 30 different topics from wet workshops to short seminars. CEUs will be granted for all sessions. 32 vendors are participating in our Exhibit Area. Your registration fee includes free admission to the Vendor Exhibit, AM and PM Breaks, buffet lunches and Friday evening's Fiesta. The hotel provides free breakfast and a shuttle to the casinos only minutes away. Come for the education, come to see the vendor exhibits, come to net-work for new jobs...and come for some fun at the Jersey Shore! You can even bring a friend! See you there! From cbarone <@t> NEMOURS.ORG Mon Jun 7 09:48:59 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon Jun 7 09:49:04 2010 Subject: [Histonet] added: to previous submission Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7D7A@wlmmsx01.nemours.org> Region II symposium: Download a meeting brochure from the NSH website under state meetings at www.nsh.org/content/region-ii-meeting. June 10,11 and 12- Atlantic City NJ From Kim.Donadio <@t> bhcpns.org Mon Jun 7 10:39:13 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Mon Jun 7 10:39:29 2010 Subject: [Histonet] Lot numbers In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB2668@HPEMX3.HealthPartners.int> Message-ID: We use a spread sheet that everyone has access to. We also have hard copies in our offsite frozen section labs for when we take new reagents over there. I have found it's a good idea to rotate the delegation of these QC task. That way every day it is assigned to someone who has the responsibility of making sure it gets done and signed off on. I add this task to our schedule. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Webb, Dorothy L" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/07/2010 09:13 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] Lot numbers Would anyone be willing to share with me how you record lot numbers of all reagents and histology components as they are used? I have a couple of ideas on how to capture this, but, they seem too detailed!! Obviously, our IHC and special stains done on the automated stainer are documented by the equipment, but, looking at all the hand stains, H&E's, processors, etc.,etc!! Thank you for any guidance in this area!! Dorothy Webb, HT Regions Histology Technical Superviosr 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From mturner <@t> carisdx.com Mon Jun 7 11:16:53 2010 From: mturner <@t> carisdx.com (Turner, Mark) Date: Mon Jun 7 11:17:00 2010 Subject: [Histonet] CD52 Message-ID: Anyone out there have any luck with the CD52 stain on any platform? Can you share your protocol? Mark Turner, HT(ASCP) QIHC Target Now IHC Supervisor Caris Life Sciences 4610 S. 44th Place Phoenix, AZ 85040 Cell: 602-309-5084 Direct 602-464-7513 Fax: 602-464-7660 mturner@carisls.com From brian <@t> prometheushealthcare.com Mon Jun 7 11:35:02 2010 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Mon Jun 7 11:35:15 2010 Subject: [Histonet] New Histology Opening in Corpus Christi, TX Message-ID: <007c01cb065f$612e11a0$238a34e0$@com> The largest provider of anatomic pathology services in South Texas, is currently seeking a histotech Early shift - start time either 3, 4, or 5am Excellent health insurance offered. This is a full time, permanent position. Contact me today for details Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From conniegrubaugh <@t> hotmail.com Mon Jun 7 16:25:14 2010 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Mon Jun 7 16:25:18 2010 Subject: [Histonet] Has Richard Allan Hematoxylin 1 been discontinued by ThermoScientific In-Reply-To: <706dbcc1fc672513529d6e57cbcf48b1.squirrel@webmail.rci.rutgers.edu> References: <000001cb059c$06ce1fc0$146a5f40$@callis@bresnan.net>, <706dbcc1fc672513529d6e57cbcf48b1.squirrel@webmail.rci.rutgers.edu> Message-ID: I have to agree about Thermoscientific website just a night mare!!!!! Connie G. > Date: Mon, 7 Jun 2010 10:17:08 -0400 > From: kgrobert@rci.rutgers.edu > To: gayle.callis@bresnan.net > Subject: Re: [Histonet] Has Richard Allan Hematoxylin 1 been discontinued by ThermoScientific > CC: histonet@lists.utsouthwestern.edu > > Just saw this email now. Here it is: > > http://www.fishersci.com/wps/portal/PRODUCTDETAIL?prodcutdetail=%27prod%27&productId=5001566&catalogId=29103&matchedCatNo=22050112&pos=7&catCode=HC_SC&endecaSearchQuery=%23store%3DHealthCare%23N%3D0%23rpp%3D15&fromCat=yes&keepSessionSearchOutPut=true&fromSearch=Y&searchKey=richards||hematoxylin||Allan||Richard&highlightProductsItemsFlag=Y > > > > Hope this helps, > Kathleen > > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (732) 445-6914 > > > Has Richard Allan Hematoxylin 1 been discontinued by ThermoScientific, > > after > > all they own RA? I am helping put together a supply list for > > laboratory, > > and am having trouble finding this on Thermo Scientific website. I did > > not > > see it on the Fisher Heathcare site either. Personally, and I hate to > > complain, but I am finding Thermo's website a nightmare to navigate. I > > may > > have to go to VWR and Cardinal who seem to have it, then why not Thermo? > > > > > > > > Impatiently, > > > > > > > > Gayle M. Callis > > > > HTL/HT/MT(ASCP) > > > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2 From steve <@t> myelinrepair.org Mon Jun 7 17:25:55 2010 From: steve <@t> myelinrepair.org (Steve Wong) Date: Mon Jun 7 17:19:34 2010 Subject: [Histonet] ISO a recommendation for a good CNS histo CRO Message-ID: Hello, I am looking for a recommendation for a CRO that is highly skilled in the art of spinal cord and brain processing, cutting, and staining (LFB, Solochrome C, MBP antibody, etc). Optimally able to process up to 40 samples at once (50 micron thick) in a single block, but the ability to process at least this number of samples in any way is acceptable. West coast preferred, but not required. From steve <@t> myelinrepair.org Mon Jun 7 17:25:56 2010 From: steve <@t> myelinrepair.org (Steve Wong) Date: Mon Jun 7 17:19:41 2010 Subject: [Histonet] ISO a recommendation for a good CNS histo CRO Message-ID: Hello, I am looking for a recommendation for a CRO that is highly skilled in the art of spinal cord and brain processing, cutting, and staining (LFB, Solochrome C, MBP antibody, etc). Optimally able to process up to 40 samples at once (50 micron thick) in a single block, but the ability to process at least this number of samples in any way is acceptable. West coast preferred, but not required. From tmoore9k <@t> gmail.com Mon Jun 7 20:45:59 2010 From: tmoore9k <@t> gmail.com (Teresa Moore) Date: Mon Jun 7 20:46:04 2010 Subject: [Histonet] Pay scale for recent grad in Columbus, Ohio area Message-ID: Can anyone tell me what kind of hourly wage to expect for a new graduate (certified histotechnician) in the Greater Columbus, Ohio area? Your assistance is appreciated. Terry M. From raj <@t> bluemarble.net Mon Jun 7 21:12:50 2010 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Mon Jun 7 21:13:06 2010 Subject: [Histonet] Embedding Centers Message-ID: <3CF3824848564906A9380588281A79AA@CHURCH> I am in the market for a new embedding center. What is everyone preference? We now have a old Shandon and a Microm. Thanks for your advice. Becky From deliadfam <@t> yahoo.com Mon Jun 7 22:44:26 2010 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Mon Jun 7 22:44:30 2010 Subject: [Histonet] Paraplast In-Reply-To: References: Message-ID: <45210.2778.qm@web63108.mail.re1.yahoo.com> We use Richard Allan Paraffin type 3 for infiltration and type 6 for embedding. We cut at 3 mircrons and we ribbon out real nice! ________________________________ From: Rebecca Johnson To: histonet Sent: Thu, June 3, 2010 1:20:37 PM Subject: [Histonet] Paraplast What paraffin is everyone using. We have been a Paraplast user made by McCormick. We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress. I have tried the a different angle at 3 with no difference. I would appreciate some help from all of you. Thanks Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Tue Jun 8 06:38:24 2010 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Jun 8 06:38:27 2010 Subject: [Histonet] VIP6 Message-ID: <924940.56252.qm@web57805.mail.re3.yahoo.com> Hi all. Anyone out?there with a VIP?6 using the cassette count as a way of tracking to determine?reagent changes? Just curious as to what?number of cassettes are recommended before changes. We're used to changing?by number of runs vs?number of cassettes.?Thanks in advance! ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From BenatM <@t> gosh.nhs.uk Tue Jun 8 09:33:48 2010 From: BenatM <@t> gosh.nhs.uk (Malika Benatti) Date: Tue Jun 8 09:34:28 2010 Subject: [Histonet] Gordon & Sweet Reticulin Stain problem on BMT Sections Message-ID: <4C0E62E8.4626.0038.0@gosh.nhs.uk> ** Proprietary ** ** Reply Requested When Convenient ** Dear all, Lately we have been experiencing problem with our Retic Stains, who keep lifting off slides after the gold chloride step. We have changed all solutions one by one, trying to see which one was causing problem without success. BMT Bx are cut at 3 micron on X-tra adhesive slides. BMT BX are decal in 10 % Formic Formaldehyde. Here is the protocol that we are currently using. 1. Sections to water. 2. Treat with 0.25% acidified Potassium permanganate. --- 5 mins 3. Bleach in oxalic acid. 4. Rinse in distilled water. 5. Treat with 5% iron alum. --- 15 mins 6. Rinse briefly in distilled water. 7. Impregnate with filtered ammoniacal silver solution ---1 min NB: Time in ammoniacal silver may need to be increased up to 5 min for bone marrow trephines 8. Rinse well in distilled water. 9. Reduce in 10% formalin (in tap water). --- 1min 10. Rinse in distilled water. 11. Tone in 0.2% Gold Chloride ---1 min 12. Rinse in distilled water. 13. Fix in 5% sodium thiosulphate (hypo) --- 30 sec 14. Wash in water. 15. Counterstain with neutral red. 16. Dehydrate, clear and mount. Any suggestions would be appreciated. Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* From Joyce.Cline <@t> wchsys.org Tue Jun 8 09:27:55 2010 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Tue Jun 8 09:35:36 2010 Subject: [Histonet] block disposal Message-ID: How does everyone handle disposing of blocks that are more than 10 years old? Are they incinerated, hauled away by a waste management company or just thrown in the trash. In Maryland I was told they can't be thrown in the regular trash by a state waste official. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From cbarone <@t> NEMOURS.ORG Tue Jun 8 09:38:37 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Tue Jun 8 09:38:50 2010 Subject: [Histonet] What's up at the Region II Symposium, June 10,11,12? Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7D84@wlmmsx01.nemours.org> Histonetters:Here are the sessions open for your interest - Region II Symposium: * Thursday June 10: Region II Registration opens for registration at 12:30: Session 1: Trouble shooting Histology Stains, Session 2 Manager's Forum, Friday June 11, AM -Session 1: VIR Seminars, Session 2: Sectioning Artifacts, Session 3: Going LEAN: LEAN to Green, Session 4: Validation/Troubleshooting IHC * Venors open at noon, Lunch * PM - Session 1: Clinical Seminars.,Session 2: Cardio System; Form and Function, Session 3. Principles of LEAN live simulation, Session 4: Ergononmics f * Saturday 12, AM, Session 1: Safety, Session 2: Prostate Cancer Markers, Session 3: Ultramicrotomy, Session 4: CAP Accreditation * Saturday PM, Session 1: Open Seminars, Session 2: Preparing for the HT/HTL, Seeion 3: Cytoprep Techniques, Session 4: Polymer Revolution in IHC Friday and Satuday seessions begin at 8:00 AM ..over 30 vendors, Guest Speaker: Peggy Wenk, and Friday evening net-working - FIESTA!!!! Walk-ins will be accepted with no late fees! So Come on down to the Shore! More info available @ www.nsh.org/content/region-ii-meeting From laurie <@t> conxis.com Tue Jun 8 10:20:46 2010 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Tue Jun 8 10:20:58 2010 Subject: [Histonet] Histodeck Flash Cards Message-ID: <69B641EF1B5F431B89D9D368A7CA5E40.MAI@accuwebhosting.biz> Hi Everyone, I was wondering if anyone out there has used the Histodeck flash cards to study for the HTL exam? Just curious. Thanks, Laurie *********************** From MSHERWOOD <@t> PARTNERS.ORG Tue Jun 8 10:35:31 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Jun 8 10:37:33 2010 Subject: [Histonet] block disposal In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E243BC@PHSXMB30.partners.org> We are a research laboratory. Our policy, based on communication via the Histonet, is to keep 2 years worth of paraffin blocks in the lab. Blocks greater than 2 years old up to 10 years old, we send to off-site storage. Blocks greater than 10 years old are destroyed by the storage company since they have them. When we first set up this system last year, we had 20 years worth of blocks and the majority were put in our red hazardous waste bucket to be incinerated. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, June 08, 2010 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block disposal How does everyone handle disposing of blocks that are more than 10 years old? Are they incinerated, hauled away by a waste management company or just thrown in the trash. In Maryland I was told they can't be thrown in the regular trash by a state waste official. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rjbuesa <@t> yahoo.com Tue Jun 8 10:48:52 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 8 10:49:04 2010 Subject: [Histonet] Gordon & Sweet Reticulin Stain problem on BMT Sections In-Reply-To: <4C0E62E8.4626.0038.0@gosh.nhs.uk> Message-ID: <799044.65814.qm@web65715.mail.ac4.yahoo.com> Usually an excess of ammonium in the amoniacal-silver solution is the culprit. Check this solution. Ren? J. --- On Tue, 6/8/10, Malika Benatti wrote: From: Malika Benatti Subject: [Histonet] Gordon & Sweet Reticulin Stain problem on BMT Sections To: histonet@lists.utsouthwestern.edu Date: Tuesday, June 8, 2010, 10:33 AM ** Proprietary ** ** Reply Requested When Convenient ** Dear all, Lately we have been experiencing problem with our Retic Stains, who keep lifting off slides after the gold chloride step. We have changed all solutions one by one, trying to see which one was causing problem without success. BMT Bx are cut at 3 micron on X-tra adhesive slides. BMT BX are decal in 10 % Formic Formaldehyde. Here is the protocol that we are currently using. 1.???Sections to water. 2.???Treat with 0.25% acidified Potassium permanganate.?????? --- 5 mins 3.???Bleach in oxalic acid. 4.???Rinse in distilled water. 5.???Treat with 5% iron alum.??? ??? ??? ? ? ? ? ? ? ? ? --- 15 mins??? ??? 6.???Rinse briefly in distilled water. 7.???Impregnate with filtered ammoniacal silver solution? ? ? ? ? ---1 min NB: Time in ammoniacal silver may need to be increased up to 5 min for bone marrow trephines 8.???Rinse well in distilled water. 9.???Reduce in 10% formalin (in tap water).??? ? ? ? ? ? ? ? ? --- 1min 10.? Rinse in distilled water. 11.? Tone in 0.2% Gold Chloride??? ??? ? ? ? ? ? ? ? ???---1 min 12.? Rinse in distilled water. 13.? Fix in 5%? sodium thiosulphate (hypo)??? ? ? ? ? ? ? ? ? --- 30 sec 14.? Wash in water. 15.? Counterstain with neutral red. 16.? Dehydrate, clear and mount. Any suggestions would be? appreciated. Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel:? +44 20 7405 9200 ext 5475 Fax:? +44 20 7829 7875 benatm@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jbaez <@t> interscopepath.com Tue Jun 8 10:51:40 2010 From: jbaez <@t> interscopepath.com (Baez, Janet) Date: Tue Jun 8 10:55:00 2010 Subject: [Histonet] block disposal Message-ID: <9E956D8FEB06C2408B08AC16498325E9255CE6@scopemx1.interscope.com> We donate our blocks to research. Find out if any institution in your area wants them.... if not we have to incinerate them by California law. Janet Baez Interscope Pathology Medical Group -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, June 08, 2010 7:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block disposal How does everyone handle disposing of blocks that are more than 10 years old? Are they incinerated, hauled away by a waste management company or just thrown in the trash. In Maryland I was told they can't be thrown in the regular trash by a state waste official. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> shorememorial.org Tue Jun 8 11:06:27 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Tue Jun 8 11:08:46 2010 Subject: [Histonet] block disposal In-Reply-To: Message-ID: We double bag our blocks in Bio Hazard bags and then they are incinerated. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Joyce Cline To Sent by: "histonet@lists.utsouthwestern.edu" histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] block disposal 06/08/2010 10:27 AM How does everyone handle disposing of blocks that are more than 10 years old? Are they incinerated, hauled away by a waste management company or just thrown in the trash. In Maryland I was told they can't be thrown in the regular trash by a state waste official. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Tue Jun 8 11:27:58 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Jun 8 11:28:07 2010 Subject: [Histonet] water bath information Message-ID: <309187.37843.qm@web50906.mail.re2.yahoo.com> Hi Guys- ? We need new waterbaths and I'm looking for a specific type.? It's blue with a square glass baking dish inset on the top.? We don't care if they're blue but the small footprint with the glass insert is the key-- ? Vendors and refurb dealer responses welcome- ? Thanks! ? Cheryl Kerry, HT(ASCP) Houston kerryc@labcorp.com From jstaruk <@t> masshistology.com Tue Jun 8 11:51:00 2010 From: jstaruk <@t> masshistology.com (jstaruk) Date: Tue Jun 8 11:51:05 2010 Subject: [Histonet] block disposal In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E243BC@PHSXMB30.partners.org> Message-ID: <0D7A7EC6D8CB473BAE860DBB7B08311A@JimPC> We are also strictly research and avoid this situation by returning all blocks back to the clients. _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, June 08, 2010 11:36 AM To: Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block disposal We are a research laboratory. Our policy, based on communication via the Histonet, is to keep 2 years worth of paraffin blocks in the lab. Blocks greater than 2 years old up to 10 years old, we send to off-site storage. Blocks greater than 10 years old are destroyed by the storage company since they have them. When we first set up this system last year, we had 20 years worth of blocks and the majority were put in our red hazardous waste bucket to be incinerated. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, June 08, 2010 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block disposal How does everyone handle disposing of blocks that are more than 10 years old? Are they incinerated, hauled away by a waste management company or just thrown in the trash. In Maryland I was told they can't be thrown in the regular trash by a state waste official. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Checked by AVG - www.avg.com Version: 9.0.829 / Virus Database: 271.1.1/2916 - Release Date: 06/08/10 02:35:00 From DKBoyd <@t> chs.net Tue Jun 8 12:58:52 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Jun 8 12:59:04 2010 Subject: [Histonet] block disposal In-Reply-To: Message-ID: We "red bag" (biohazard) ours and they are incinerated. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Ken_Marissael <@t> vwr.com Tue Jun 8 13:50:08 2010 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Tue Jun 8 13:50:13 2010 Subject: [Histonet] Away for instrument training. Message-ID: I will be out of the office starting 06/08/2010 and will not return until 06/14/2010. I will be out of the field beginning June 8th and will return on June 14th. Please call Customer Service at 1-800-932-5000 for any of your immediate needs. I will have very limited access to e-mail and v-mail, but will try to respond as quickly as I can. From cpyse <@t> x-celllab.com Tue Jun 8 14:02:08 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Jun 8 14:03:29 2010 Subject: [Histonet] water bath information In-Reply-To: <309187.37843.qm@web50906.mail.re2.yahoo.com> References: <309187.37843.qm@web50906.mail.re2.yahoo.com> Message-ID: <000901cb073d$181d3d40$4857b7c0$@com> I am also looking for the same type of waterbaths and would appreciate any information. Thanks Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 08, 2010 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] water bath information Hi Guys- ? We need new waterbaths and I'm looking for a specific type.? It's blue with a square glass baking dish inset on the top.? We don't care if they're blue but the small footprint with the glass insert is the key-- ? Vendors and refurb dealer responses welcome- ? Thanks! ? Cheryl Kerry, HT(ASCP) Houston kerryc@labcorp.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From janderson <@t> halozyme.com Tue Jun 8 14:34:43 2010 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Tue Jun 8 14:35:04 2010 Subject: [Histonet] Refrigerator Mold Message-ID: <5F7CC9B788911848A79BC83453A3D65304116DEF5D@Tomlinson.hti.com> Hello and Greetings. I am looking for a solution to our refrigerator mold problem. We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. From sbreeden <@t> nmda.nmsu.edu Tue Jun 8 14:34:13 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jun 8 14:37:40 2010 Subject: [Histonet] Waterbath Options Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47148@nmdamailsvr.nmda.ad.nmsu.edu> Okay, time for my two cents' worth. Water baths - I don't like the glass-dish-insert kind because water can get beneath the glass dish and can temp fluctuations. And if the glass insert breaks, it might be difficult to find a replacement (Pyrex?). I like the good ol' fashioned round or rectangular black-lined fill-it-with-water-and-turn-it-on kind. They hold temp much better and because they are lined with something like black Teflon (or whatever), they clean like a dream. I found that I really do not like the romantic under-tissue lighting. It's all about preferences and this is mine...not that you asked, but that never stopped me before, did it? Happy Tuesday! Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 From ttruscot <@t> vetmed.wsu.edu Tue Jun 8 14:39:46 2010 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Tue Jun 8 14:39:59 2010 Subject: [Histonet] water bath information In-Reply-To: <000901cb073d$181d3d40$4857b7c0$@com> References: <309187.37843.qm@web50906.mail.re2.yahoo.com> <000901cb073d$181d3d40$4857b7c0$@com> Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB401134BAFFD49@CVMMBX.vetmed.wsu.edu> Hi, The old TBS's are blue, and the new ones are white. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, June 08, 2010 12:02 PM To: 'Cheryl'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] water bath information I am also looking for the same type of waterbaths and would appreciate any information. Thanks Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 08, 2010 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] water bath information Hi Guys- ? We need new waterbaths and I'm looking for a specific type.? It's blue with a square glass baking dish inset on the top.? We don't care if they're blue but the small footprint with the glass insert is the key-- ? Vendors and refurb dealer responses welcome- ? Thanks! ? Cheryl Kerry, HT(ASCP) Houston kerryc@labcorp.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Tue Jun 8 15:08:31 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Jun 8 15:08:38 2010 Subject: [Histonet] Refrigerator Mold In-Reply-To: <5F7CC9B788911848A79BC83453A3D65304116DEF5D@Tomlinson.hti.com> References: <5F7CC9B788911848A79BC83453A3D65304116DEF5D@Tomlinson.hti.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E243C5@PHSXMB30.partners.org> By folders, I assume you mean the cardboard ones. Why not store the slides in something else, i.e. plastic boxes? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Tuesday, June 08, 2010 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerator Mold Hello and Greetings. I am looking for a solution to our refrigerator mold problem. We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From JMacDonald <@t> mtsac.edu Tue Jun 8 15:42:22 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jun 8 15:42:30 2010 Subject: [Histonet] Refrigerator Mold In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E243C5@PHSXMB30.partners.org> Message-ID: Bleach can cause corrosion of the metal parts. "Sherwood, Margaret " Sent by: histonet-bounces@lists.utsouthwestern.edu 06/08/2010 01:36 PM To "Jennifer Anderson" , cc Subject RE: [Histonet] Refrigerator Mold By folders, I assume you mean the cardboard ones. Why not store the slides in something else, i.e. plastic boxes? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Tuesday, June 08, 2010 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerator Mold Hello and Greetings. I am looking for a solution to our refrigerator mold problem. We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Tue Jun 8 15:43:26 2010 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Tue Jun 8 15:43:58 2010 Subject: [Histonet] Special Stain Procedures Message-ID: <8CCD56260E1D992-1218-2604@webmail-m094.sysops.aol.com> Does anyone have the following procedures they can share with me: - Rhodamine (for copper) - Victoria Blue Thank you, Ann From JWeems <@t> sjha.org Tue Jun 8 16:41:15 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jun 8 16:41:56 2010 Subject: [Histonet] RE: Refrigerator Mold In-Reply-To: <5F7CC9B788911848A79BC83453A3D65304116DEF5D@Tomlinson.hti.com> References: <5F7CC9B788911848A79BC83453A3D65304116DEF5D@Tomlinson.hti.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DD6C205@CHEXCMS10.one.ads.che.org> Scrape off the mold and make us all some control blocks!! ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson [janderson@halozyme.com] Sent: Tuesday, June 08, 2010 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerator Mold Hello and Greetings. I am looking for a solution to our refrigerator mold problem. We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From kc <@t> ka-recruiting.com Tue Jun 8 16:42:06 2010 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Tue Jun 8 16:42:16 2010 Subject: [Histonet] Histology Jobs Message-ID: <1116999842.1276033325915.JavaMail.cfservice@sl4app2> Hello Everyone, I am one of the owners of a healthcare recruiting firm that specializes in placing Lab Professionals and I wanted to see if you'd be interested in learning about potential career advancing job opportunities? We are completely free of charge to candidates and can be a great resource for you in helping you to find your next job. Our clients offer competitive compensation packages and typically assist with relocation expenses. I am currently working on some great job opportunities in various areas across the country. One position in particular you may be interested in is a 1st shift Histotechnologist job located outside of Indianapolis. This opportunity is with a 250 bed hospital that is consistently ranked as being one of the top 10 employers in Indiana. My client is offering an above average compensation package, excellent benefits and relocation assistance when necessary. Qualified candidates must be HT or HTL (ASCP) certified with prior experience working in a clinical lab setting. Please let me know if you're be interested in hearing more about this position and learning if it might be the next step in your career. Below is a list of some of the opportunities we're currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential. IN - Columbus - Histotechnologist (1st Shift) *****New Opening***** GA - Histology Supervisor NY- Upstate- Histotech (1st Shift) NY- New York City - Histology Supervisor NY - New York City - Histotech (3rd shift) NY - New York City - Grossing Technologist *****New Opening***** NV -Las Vegas - Histotech (3rd shift) NV Las Vegas - Histology Supervisor (3rd shift) CA - Southern - Histotech GA - Atlanta - Histotech MA - Histotech MA - IHC Tech If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right time for you to make a career change then please let me know if you can refer someone who is. We offer a generous referral bonus for anyone you refer to us that we place into any position across the country. To learn more about us please visit our website at www.ka-recruiting.com. Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From JWeems <@t> sjha.org Tue Jun 8 16:42:01 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jun 8 16:43:05 2010 Subject: [Histonet] RE: Refrigerator Mold In-Reply-To: <5F7CC9B788911848A79BC83453A3D65304116DEF5D@Tomlinson.hti.com> References: <5F7CC9B788911848A79BC83453A3D65304116DEF5D@Tomlinson.hti.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DD6C206@CHEXCMS10.one.ads.che.org> oops = didn't intend to hit send.. But perhaps try dessicant or baking soda, which would reduce the moisture.... ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson [janderson@halozyme.com] Sent: Tuesday, June 08, 2010 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerator Mold Hello and Greetings. I am looking for a solution to our refrigerator mold problem. We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From ddreesen <@t> sbcglobal.net Tue Jun 8 16:56:50 2010 From: ddreesen <@t> sbcglobal.net (Debbie Dreesen) Date: Tue Jun 8 16:57:00 2010 Subject: [Histonet] Re: Block Disposal In-Reply-To: <201006081741.o58HfhGX030749@flpd119.prodigy.net> Message-ID: <49117.29704.qm@web81002.mail.mud.yahoo.com> Joyce, We disposed of of tissues and their containers after 6 weeks on the shelf. Outdated blocks were placed in the same totes used for the tissues and picked up by an outside company for incineration. Debbie Dreesen, HT(ASCP) Message: 9 Date: Tue, 8 Jun 2010 10:27:55 -0400 From: Joyce Cline Subject: [Histonet] block disposal To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? Content-Type: text/plain; charset="iso-8859-1" How does everyone handle disposing of blocks that are more than 10 years old? Are they incinerated, hauled away by a waste management company or just thrown in the trash. In Maryland I was told they can't be thrown in the regular trash by a state waste official. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@wchsys.orgmailto:joyce.cline@wchsys.org From ddreesen <@t> sbcglobal.net Tue Jun 8 17:49:20 2010 From: ddreesen <@t> sbcglobal.net (Debbie Dreesen) Date: Tue Jun 8 17:49:27 2010 Subject: [Histonet] Re: Leica Paraplast In-Reply-To: <201006041722.o54HM6M1017855@nlpi091.prodigy.net> Message-ID: <981456.12795.qm@web81004.mail.mud.yahoo.com> Hi Becky, There were several posts between March 23-31 addressing the quality of the Paraplast if you want to check them out.?We tried trouble-shooting everything we could think of when we noticed a change in the paraffin.?The texture was very different?and it was so?sticky?our sections stuck to the blade no matter how we tried to fix the problem.?It was gritty and caused nicks in the blade after just a few sections. We tried several different blades to make sure it wasn't a problem there or a bad batch.?Different brands as well as both low?and high profile. We?tried the different blades and settings because we had just replaced all our microtomes?and thought maybe we just needed to make some adjustments but we experienced the same problem with each?blade at every setting we tried?and concluded the paraffin was the problem.?The issues?were resolved after we switched back to ordering the EM-400 from Cardinal. Debbie Dreesen, HT(ASCP) Message: 3 Date: Thu, 3 Jun 2010 15:20:37 -0500 From: "Rebecca Johnson" Subject: [Histonet] Paraplast To: "histonet" Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; ??? reply-type=original What paraffin is everyone using. We have been a Paraplast user made by McCormick.? We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress.? I have tried the a different angle at 3 with no difference.? I would appreciate some help from all of you. Thanks Becky From mshaeffer <@t> cox.net Tue Jun 8 18:19:43 2010 From: mshaeffer <@t> cox.net (Marc & Sandy Shaeffer) Date: Tue Jun 8 18:19:46 2010 Subject: [Histonet] Oncotech Message-ID: <6218769.30833.1276039183117.JavaMail.mshaeffer@127.0.0.1> Amy Senn asked: Now that Oncotech is out of business, who is everyone sending their extreme resistance assays to? We were just briefed today by a company called Precision Therapeutics out of Pittsburgh PA that they perform Chemo sensitivity and resistance tests and the test is called ChemoFx. Their website is www.precisiontherapeutics.com. I have no affiliation with this company. Precision Therapeutics supplies the shipping container and fluid, usually overnight delivery with Saturday deliveries allowed. Marc Shaeffer Tucson Medical Center Arizona From stevenhacker <@t> verizon.net Tue Jun 8 18:54:11 2010 From: stevenhacker <@t> verizon.net (Steven Hacker) Date: Tue Jun 8 18:54:19 2010 Subject: [Histonet] block disposal Message-ID: <1232490361.717331.1276041251677.JavaMail.root@vms246.mailsrvcs.net> Hey, Joyce... Contact some of your vendors...Many of them would love to get addition companies will d compensate/donate to th difference in future assays. Regards, Steven Hacker Ps...Yes, I work for Biocare Medical and we're always interested in bl prefer to kn and if they were po information will ever be accepted.< Jun 8, 2010 01:38:11 PM, jstaruk@masshistology.com wrote: < We are also s returning all blocks back t _______________________ James E. Staruk HT(ASCP)www.masshistology.com www.nehorselabs.com -----Orig From: histonet-bounces@lists.utsouthwestern.edu [ma Sherwood, M Sent: Tuesday, June 08, 2010 11:36 AM To: Joyce Cline; histo Subject: RE: [Histonet] block disposal < We are a research laboratory. Our policy, based on communication via Histonet, is to keep 2 years worth of paraffin blocks in the lab. B greater than 2 years old up to 10 years old, we send to off-site s torage. Blocks greater than 10 years old are destroyed by the storage co mpany since they have them. When we first set up this system last of blocks and the majority were put in our incinerated. Peggy From: histonet-bounces@lists.utsouthwestern.e [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Sent: Tuesday, June 08, 2010 10:28 AM To: histonet@lists.utsou Subject: [Histonet] block disposal How does everyon 10 years old? Are they i company or just thrown in In Maryland I was told they can't be thrown in the regula by a state waste official. Joyce Cline, Technical Special Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 jo ________________________________ ***** CONFIDENT confidential information and i are not the named addre copy this e-mail. Please have received this e-mail ___________________ Histonet mailing list Histonet@lists.uts http://lists.utsouthwestern.edu/mailman/listinfo/histone The information in this e-mail is intended only for the person whom it is addressed. If you believe this e-mail was sent to you in and the e-mail contains patient information, please contact the Pa Compliance HelpLine at http://www.partners.org/complianceline . I e-mail was sent to you in error but does not contain patient infor contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailin Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern. Checked by AVG - www.avg.com Versi 06/08/10 02:3 _______________________________________________ Histonet Histonet@lists.utsouthwestern.edu http://lists.utsouthw From brandihiggins <@t> gmail.com Tue Jun 8 18:59:31 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Tue Jun 8 18:59:41 2010 Subject: [Histonet] Oncotech In-Reply-To: References: Message-ID: Hello, Many of our surgeons who previously requested we send specimens to Oncotech switched to Precision Therapeutics about a year or so ago (although some still used Oncotech at times). We have used Precision with no problems. They even supplied us with a refrigerator to store the specimen collection kits which need to be refrigerated, and our reps have been very helpful overall. Hope this helps. Brandi Higgins, HT(ASCP) On Mon, Jun 7, 2010 at 9:27 AM, Senn, Amy R wrote: > Hello Histonet, > > > > > > Now that Oncotech is out of business, who is everyone sending their > extreme resistance assays to? > > > > > > Thanks, > > Amy, HT > > Camp Hill, PA > > > > > > > > > > > > Attention: This Message is intended only for the use of the individual or > entity to which it is addressed, and may contain information that is > privileged, confidential and exempt from disclosure under applicable law. If > the reader of this message is not the intended recipient, you are hereby > notified that any dissemination or copying of this message or the taking of > any action in reliance on the contents of this message is strictly > prohibited. If you have received this message in error, please notify us > immediately and destroy the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From HornHV <@t> archildrens.org Wed Jun 9 07:07:39 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Jun 9 07:07:45 2010 Subject: [Histonet] Special Stain Procedures In-Reply-To: <8CCD56260E1D992-1218-2604@webmail-m094.sysops.aol.com> References: <8CCD56260E1D992-1218-2604@webmail-m094.sysops.aol.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D838AA@EMAIL.archildrens.org> For copper we use the kit from American Master Tech. It works really well. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com Sent: Tuesday, June 08, 2010 3:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Procedures Does anyone have the following procedures they can share with me: - Rhodamine (for copper) - Victoria Blue Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From jshelley <@t> burnham.org Wed Jun 9 07:21:50 2010 From: jshelley <@t> burnham.org (John Shelley) Date: Wed Jun 9 07:22:02 2010 Subject: [Histonet] RNA FISN on frozen tissue samples Message-ID: Hi Histonetters, I have a huge favor to ask, I have been asked to start up work on frozen tissue samples to perform RNA FISH. I am looking for some individuals how are doing this or attempting to do this and can help shed some light on this undertaking. We successfully were able to get this protocol to work on cultures cells form a few different lines but know that the transition may be much more difficult in human tissue samples. Again any help or advise anyone can give would be greatly appreciated. If you want to contact me offline that would be fine. Thanks in advance for any help. John From rjbuesa <@t> yahoo.com Wed Jun 9 08:01:44 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 9 08:01:56 2010 Subject: [Histonet] Special Stain Procedures In-Reply-To: <8CCD56260E1D992-1218-2604@webmail-m094.sysops.aol.com> Message-ID: <929438.23665.qm@web65704.mail.ac4.yahoo.com> Neither procedure is reliable. Use Tim's method (sulfur dioxide). Ren? J. --- On Tue, 6/8/10, thisisann@aol.com wrote: From: thisisann@aol.com Subject: [Histonet] Special Stain Procedures To: histonet@lists.utsouthwestern.edu Date: Tuesday, June 8, 2010, 4:43 PM Does anyone have the following procedures they can share with me: - Rhodamine (for copper) - Victoria Blue Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jun 9 08:08:21 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 9 08:08:31 2010 Subject: [Histonet] Refrigerator Mold In-Reply-To: <5F7CC9B788911848A79BC83453A3D65304116DEF5D@Tomlinson.hti.com> Message-ID: <316872.16387.qm@web65706.mail.ac4.yahoo.com> You should never use an open container with bleach in any place where you keep slides, because bleach will affect the antigens in the sections and affect histochemical reactions as well. Carboard folders will catch fungus spores outside the refrigerator that eventually will develop into fungi, as in your case. Carboard folders are not a good option to store slides in the humid environment of a refrigerator. You should put your slides in plastic boxes, either in large ones (100 slides each) or small ones (25 slides). You will be able alto to store more slides in less space. Ren? J. ? ? --- On Tue, 6/8/10, Jennifer Anderson wrote: From: Jennifer Anderson Subject: [Histonet] Refrigerator Mold To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, June 8, 2010, 3:34 PM Hello and Greetings. I am looking for a solution to our refrigerator mold problem.? We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Jun 9 08:41:50 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jun 9 08:41:58 2010 Subject: [Histonet] Explanted prosthetic valves Message-ID: <4C0F61DE.7400.0077.1@harthosp.org> For those of you who work in hospitals: What is your policy on explanted prosthetic valves? Discard or send to pathology? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From bliven.laura <@t> marshfieldclinic.org Wed Jun 9 08:47:09 2010 From: bliven.laura <@t> marshfieldclinic.org (Bliven, Laura) Date: Wed Jun 9 08:47:25 2010 Subject: [Histonet] Image Analysis Message-ID: <201006091347.o59DlBca021717@spamfilt> What software systems is everyone in clinical settings using for image analysis of markers such as p53, MIB1, Estrogen, Progesterone, HER2 IHC, possibly and HER2 FISH for starters? What characteristics do you like and not like about your system? Thanks for time and feedback. Feedback is so valuable in everyone's job, no matter where you work. Laura Laura Bliven, AAS, HT(ASCP), QIHC Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From HornHV <@t> archildrens.org Wed Jun 9 08:58:53 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Jun 9 08:59:03 2010 Subject: [Histonet] Special Stain Procedures In-Reply-To: <929438.23665.qm@web65704.mail.ac4.yahoo.com> References: <8CCD56260E1D992-1218-2604@webmail-m094.sysops.aol.com> <929438.23665.qm@web65704.mail.ac4.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D838AB@EMAIL.archildrens.org> I have to disagree with Rene on this one....the Microwave Copper from American Master Tech is awesome and it's never failed. It's a rhodamine stain and we don't even use the microwave. We use a pressure cooker. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, June 09, 2010 8:02 AM To: histonet@lists.utsouthwestern.edu; thisisann@aol.com Subject: Re: [Histonet] Special Stain Procedures Neither procedure is reliable. Use Tim's method (sulfur dioxide). Ren? J. --- On Tue, 6/8/10, thisisann@aol.com wrote: From: thisisann@aol.com Subject: [Histonet] Special Stain Procedures To: histonet@lists.utsouthwestern.edu Date: Tuesday, June 8, 2010, 4:43 PM Does anyone have the following procedures they can share with me: - Rhodamine (for copper) - Victoria Blue Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From JWeems <@t> sjha.org Wed Jun 9 09:34:44 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jun 9 09:38:13 2010 Subject: [Histonet] Explanted prosthetic valves In-Reply-To: <4C0F61DE.7400.0077.1@harthosp.org> References: <4C0F61DE.7400.0077.1@harthosp.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DD6C20C@CHEXCMS10.one.ads.che.org> Send to pathology for gross description and photo. Then sent to manufacturer if requested. j Joyce Weems Saint Joseph's Hospital Pathology Dept 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - P 678-843-7831 - F ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Wednesday, June 09, 2010 9:41 AM To: Histonet Subject: [Histonet] Explanted prosthetic valves For those of you who work in hospitals: What is your policy on explanted prosthetic valves? Discard or send to pathology? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From tanisha.neely <@t> covance.com Wed Jun 9 09:55:06 2010 From: tanisha.neely <@t> covance.com (Neely, Tanisha) Date: Wed Jun 9 09:55:21 2010 Subject: [Histonet] FFPE Tissue Block Collection Container For Worldwide Shipment Message-ID: <816E3C72F855F14985FC31D7C963AE6F1E888D42@indexch03.ent.covance.com> Hello Histo-Netters: I am looking to source a temperature stable container to ship Archival FFPE Blocks in. These blocks could be shipped all around the world and subject to extreme temperatures at times. We are also wanting to limit the collection of these samples to JUST 1 BLOCK per container. Does anyone know of any containers that are made for FFPE Tissue Block Shipment and where I might find them? Tanisha N. Neely, HT (ASCP) ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From vavalos <@t> allergydermatology.com Wed Jun 9 10:53:44 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Wed Jun 9 10:53:48 2010 Subject: [Histonet] Stainer Slide Clips Message-ID: <000f01cb07eb$f14fa9f0$d3eefdd0$@com> Looking for slide clips that will be compatible for our stainers. Does anyone use different vendors other than the equipment manufacturer? We have a Thermo GLX Linear Stainer. They sell 25/$144.50 . Crazy but they are necessary! V.Avalos ADS, INC Fax:602-277-2134 From HornHV <@t> archildrens.org Wed Jun 9 11:12:18 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Jun 9 11:12:27 2010 Subject: [Histonet] Explanted prosthetic valves In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164015DD6C20C@CHEXCMS10.one.ads.che.org> References: <4C0F61DE.7400.0077.1@harthosp.org> <92AD9B20A6C38C4587A9FEBE3A30E164015DD6C20C@CHEXCMS10.one.ads.che.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D838AF@EMAIL.archildrens.org> We do the same as Joyce does. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, June 09, 2010 9:35 AM To: Richard Cartun; Histonet Subject: RE: [Histonet] Explanted prosthetic valves Send to pathology for gross description and photo. Then sent to manufacturer if requested. j Joyce Weems Saint Joseph's Hospital Pathology Dept 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - P 678-843-7831 - F ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Wednesday, June 09, 2010 9:41 AM To: Histonet Subject: [Histonet] Explanted prosthetic valves For those of you who work in hospitals: What is your policy on explanted prosthetic valves? Discard or send to pathology? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From gmartin <@t> marshallmedical.org Wed Jun 9 14:17:21 2010 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Jun 9 14:17:29 2010 Subject: [Histonet] JAHCO vs. CAP Message-ID: <6ED9D4252F278841A0593D3D788AF24C08FB7CDE@mailsvr.MARSHMED.local> We are having a discussion about switching our inspection process form CAP to JAHCO. One of our questions is, can we still participate in the proficiency testing through CAP> If anyone has done this can you explain the process and the upside and the down side. Thanks Gary M. From Aubrey <@t> nsh.org Wed Jun 9 14:27:26 2010 From: Aubrey <@t> nsh.org (Aubrey Wanner) Date: Wed Jun 9 14:28:42 2010 Subject: [Histonet] NSH IHC Forum July 17th Message-ID: Registration for the NSH One Day Immunohistochemistry Forum for Histotechs is now open. The event developed with Dr. Richard Cartun and other experts in the field is scheduled for July 17, 2010 in Ft. Lauderdale, FL. The forum will begin with an introductory session on IHC and will conclude with sessions on advanced subjects including biomarkers in breast cancer, applications of IHC to cytology specimens, kappa and lambda detection, mismatch repair staining for microsatellite instability analysis, and infectious disease detection. Thanks to our 2010 Professional Development Partners NSH is able to offer this one day forum worth 7.5 contact hours for the very low price of $119.00 to NSH members and $159 for non members. We hope you will be able to join us. You can find all relevant details & registration information online: https://www.nshonline.org/eweb/DynamicPage.aspx?webcode=EventInfo&Reg_ev t_key=32332cbe-49a2-4c68-8d33-e085aea94432&RegPath=EventRegFees If you have any questions about the program or registering please feel free to contact the NSH Office, 443-535-4060. From Goodwd2 <@t> LabCorp.com Wed Jun 9 14:30:21 2010 From: Goodwd2 <@t> LabCorp.com (Goodwin, Diana) Date: Wed Jun 9 14:35:36 2010 Subject: [Histonet] RE: Explanted prosthetic valves Message-ID: Hi, Rich. I've worked at institutions with both policies. At one, we had a detailed Explanted Medical Device Policy which was developed in cooperation with the Risk Management Department. The other left it up to the surgeon as to whether or not to send the devices to pathology. Ultimately, it is up to Risk Management to ensure compliance with explanted medical device requirements. Hope this helps. Diana Goodwin ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Wednesday, June 09, 2010 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: block disposal (DKBoyd@chs.net) 2. Away for instrument training. (Ken_Marissael@vwr.com) 3. RE: water bath information (Cynthia Pyse) 4. Refrigerator Mold (Jennifer Anderson) 5. Waterbath Options (Breeden, Sara) 6. RE: water bath information (Truscott, Tom) 7. RE: Refrigerator Mold (Sherwood, Margaret ) 8. RE: Refrigerator Mold (Jennifer MacDonald) 9. Special Stain Procedures (thisisann@aol.com) 10. RE: Refrigerator Mold (Weems, Joyce) 11. Histology Jobs (K.C. Carpenter) 12. RE: Refrigerator Mold (Weems, Joyce) 13. Re: Block Disposal (Debbie Dreesen) 14. Re: Leica Paraplast (Debbie Dreesen) 15. Oncotech (Marc & Sandy Shaeffer) 16. Re: RE: [Histonet] block disposal (Steven Hacker) 17. Re: Oncotech (Brandi Higgins) 18. RE: Special Stain Procedures (Horn, Hazel V) 19. RNA FISN on frozen tissue samples (John Shelley) 20. Re: Special Stain Procedures (Rene J Buesa) 21. Re: Refrigerator Mold (Rene J Buesa) 22. Explanted prosthetic valves (Richard Cartun) 23. Image Analysis (Bliven, Laura) ---------------------------------------------------------------------- Message: 1 Date: Tue, 8 Jun 2010 13:58:52 -0400 From: DKBoyd@chs.net Subject: Re: [Histonet] block disposal To: Joyce Cline Cc: "histonet@lists.utsouthwestern.edu" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We "red bag" (biohazard) ours and they are incinerated. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 2 Date: Tue, 8 Jun 2010 14:50:08 -0400 From: Ken_Marissael@vwr.com Subject: [Histonet] Away for instrument training. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 06/08/2010 and will not return until 06/14/2010. I will be out of the field beginning June 8th and will return on June 14th. Please call Customer Service at 1-800-932-5000 for any of your immediate needs. I will have very limited access to e-mail and v-mail, but will try to respond as quickly as I can. ------------------------------ Message: 3 Date: Tue, 8 Jun 2010 15:02:08 -0400 From: "Cynthia Pyse" Subject: RE: [Histonet] water bath information To: "'Cheryl'" , Message-ID: <000901cb073d$181d3d40$4857b7c0$@com> Content-Type: text/plain; charset="iso-8859-1" I am also looking for the same type of waterbaths and would appreciate any information. Thanks Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 08, 2010 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] water bath information Hi Guys- ? We need new waterbaths and I'm looking for a specific type.? It's blue with a square glass baking dish inset on the top.? We don't care if they're blue but the small footprint with the glass insert is the key-- ? Vendors and refurb dealer responses welcome- ? Thanks! ? Cheryl Kerry, HT(ASCP) Houston kerryc@labcorp.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 8 Jun 2010 12:34:43 -0700 From: Jennifer Anderson Subject: [Histonet] Refrigerator Mold To: "histonet@lists.utsouthwestern.edu" Message-ID: <5F7CC9B788911848A79BC83453A3D65304116DEF5D@Tomlinson.hti.com> Content-Type: text/plain; charset="us-ascii" Hello and Greetings. I am looking for a solution to our refrigerator mold problem. We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. ------------------------------ Message: 5 Date: Tue, 8 Jun 2010 13:34:13 -0600 From: "Breeden, Sara" Subject: [Histonet] Waterbath Options To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47148@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Okay, time for my two cents' worth. Water baths - I don't like the glass-dish-insert kind because water can get beneath the glass dish and can temp fluctuations. And if the glass insert breaks, it might be difficult to find a replacement (Pyrex?). I like the good ol' fashioned round or rectangular black-lined fill-it-with-water-and-turn-it-on kind. They hold temp much better and because they are lined with something like black Teflon (or whatever), they clean like a dream. I found that I really do not like the romantic under-tissue lighting. It's all about preferences and this is mine...not that you asked, but that never stopped me before, did it? Happy Tuesday! Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ------------------------------ Message: 6 Date: Tue, 8 Jun 2010 12:39:46 -0700 From: "Truscott, Tom" Subject: RE: [Histonet] water bath information To: Cynthia Pyse , 'Cheryl' , "histonet@lists.utsouthwestern.edu" Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB401134BAFFD49@CVMMBX.vetmed.wsu.edu> Content-Type: text/plain; charset="iso-8859-1" Hi, The old TBS's are blue, and the new ones are white. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, June 08, 2010 12:02 PM To: 'Cheryl'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] water bath information I am also looking for the same type of waterbaths and would appreciate any information. Thanks Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 08, 2010 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] water bath information Hi Guys- ? We need new waterbaths and I'm looking for a specific type.? It's blue with a square glass baking dish inset on the top.? We don't care if they're blue but the small footprint with the glass insert is the key-- ? Vendors and refurb dealer responses welcome- ? Thanks! ? Cheryl Kerry, HT(ASCP) Houston kerryc@labcorp.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 8 Jun 2010 16:08:31 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] Refrigerator Mold To: "Jennifer Anderson" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E243C5@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" By folders, I assume you mean the cardboard ones. Why not store the slides in something else, i.e. plastic boxes? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Tuesday, June 08, 2010 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerator Mold Hello and Greetings. I am looking for a solution to our refrigerator mold problem. We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 8 Date: Tue, 8 Jun 2010 13:42:22 -0700 From: Jennifer MacDonald Subject: RE: [Histonet] Refrigerator Mold To: "Sherwood, Margaret " Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Bleach can cause corrosion of the metal parts. "Sherwood, Margaret " Sent by: histonet-bounces@lists.utsouthwestern.edu 06/08/2010 01:36 PM To "Jennifer Anderson" , cc Subject RE: [Histonet] Refrigerator Mold By folders, I assume you mean the cardboard ones. Why not store the slides in something else, i.e. plastic boxes? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Tuesday, June 08, 2010 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerator Mold Hello and Greetings. I am looking for a solution to our refrigerator mold problem. We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 08 Jun 2010 16:43:26 -0400 From: thisisann@aol.com Subject: [Histonet] Special Stain Procedures To: histonet@lists.utsouthwestern.edu Message-ID: <8CCD56260E1D992-1218-2604@webmail-m094.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Does anyone have the following procedures they can share with me: - Rhodamine (for copper) - Victoria Blue Thank you, Ann ------------------------------ Message: 10 Date: Tue, 8 Jun 2010 17:41:15 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: Refrigerator Mold To: Jennifer Anderson , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DD6C205@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Scrape off the mold and make us all some control blocks!! ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson [janderson@halozyme.com] Sent: Tuesday, June 08, 2010 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerator Mold Hello and Greetings. I am looking for a solution to our refrigerator mold problem. We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 11 Date: 8 Jun 2010 17:42:06 -0400 From: "K.C. Carpenter" Subject: [Histonet] Histology Jobs To: histonet@lists.utsouthwestern.edu Message-ID: <1116999842.1276033325915.JavaMail.cfservice@sl4app2> Content-Type: text/plain; charset="utf-8" Hello Everyone, I am one of the owners of a healthcare recruiting firm that specializes in placing Lab Professionals and I wanted to see if you'd be interested in learning about potential career advancing job opportunities? We are completely free of charge to candidates and can be a great resource for you in helping you to find your next job. Our clients offer competitive compensation packages and typically assist with relocation expenses. I am currently working on some great job opportunities in various areas across the country. One position in particular you may be interested in is a 1st shift Histotechnologist job located outside of Indianapolis. This opportunity is with a 250 bed hospital that is consistently ranked as being one of the top 10 employers in Indiana. My client is offering an above average compensation package, excellent benefits and relocation assistance when necessary. Qualified candidates must be HT or HTL (ASCP) certified with prior experience working in a clinical lab setting. Please let me know if you're be interested in hearing more about this position and learning if it might be the next step in your career. Below is a list of some of the opportunities we're currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential. IN - Columbus - Histotechnologist (1st Shift) *****New Opening***** GA - Histology Supervisor NY- Upstate- Histotech (1st Shift) NY- New York City - Histology Supervisor NY - New York City - Histotech (3rd shift) NY - New York City - Grossing Technologist *****New Opening***** NV -Las Vegas - Histotech (3rd shift) NV Las Vegas - Histology Supervisor (3rd shift) CA - Southern - Histotech GA - Atlanta - Histotech MA - Histotech MA - IHC Tech If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right time for you to make a career change then please let me know if you can refer someone who is. We offer a generous referral bonus for anyone you refer to us that we place into any position across the country. To learn more about us please visit our website at www.ka-recruiting.com. Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 12 Date: Tue, 8 Jun 2010 17:42:01 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: Refrigerator Mold To: Jennifer Anderson , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DD6C206@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" oops = didn't intend to hit send.. But perhaps try dessicant or baking soda, which would reduce the moisture.... ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson [janderson@halozyme.com] Sent: Tuesday, June 08, 2010 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerator Mold Hello and Greetings. I am looking for a solution to our refrigerator mold problem. We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 13 Date: Tue, 8 Jun 2010 14:56:50 -0700 (PDT) From: Debbie Dreesen Subject: [Histonet] Re: Block Disposal To: histonet@lists.utsouthwestern.edu Message-ID: <49117.29704.qm@web81002.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Joyce, We disposed of of tissues and their containers after 6 weeks on the shelf. Outdated blocks were placed in the same totes used for the tissues and picked up by an outside company for incineration. Debbie Dreesen, HT(ASCP) Message: 9 Date: Tue, 8 Jun 2010 10:27:55 -0400 From: Joyce Cline Subject: [Histonet] block disposal To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? Content-Type: text/plain; charset="iso-8859-1" How does everyone handle disposing of blocks that are more than 10 years old? Are they incinerated, hauled away by a waste management company or just thrown in the trash. In Maryland I was told they can't be thrown in the regular trash by a state waste official. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@wchsys.orgmailto:joyce.cline@wchsys.org ------------------------------ Message: 14 Date: Tue, 8 Jun 2010 15:49:20 -0700 (PDT) From: Debbie Dreesen Subject: [Histonet] Re: Leica Paraplast To: histonet@lists.utsouthwestern.edu Message-ID: <981456.12795.qm@web81004.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Becky, There were several posts between March 23-31 addressing the quality of the Paraplast if you want to check them out.?We tried trouble-shooting everything we could think of when we noticed a change in the paraffin.?The texture was very different?and it was so?sticky?our sections stuck to the blade no matter how we tried to fix the problem.?It was gritty and caused nicks in the blade after just a few sections. We tried several different blades to make sure it wasn't a problem there or a bad batch.?Different brands as well as both low?and high profile. We?tried the different blades and settings because we had just replaced all our microtomes?and thought maybe we just needed to make some adjustments but we experienced the same problem with each?blade at every setting we tried?and concluded the paraffin was the problem.?The issues?were resolved after we switched back to ordering the EM-400 from Cardinal. Debbie Dreesen, HT(ASCP) Message: 3 Date: Thu, 3 Jun 2010 15:20:37 -0500 From: "Rebecca Johnson" Subject: [Histonet] Paraplast To: "histonet" Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; ??? reply-type=original What paraffin is everyone using. We have been a Paraplast user made by McCormick.? We have noticed black to gray small dusty particles in the embedding well. I have tried a couple others, but the ribbons compress.? I have tried the a different angle at 3 with no difference.? I would appreciate some help from all of you. Thanks Becky ------------------------------ Message: 15 Date: Tue, 8 Jun 2010 19:19:43 -0400 (EDT) From: Marc & Sandy Shaeffer Subject: [Histonet] Oncotech To: "histonet@lists.utsouthwestern.edu" Cc: arsenn@hsh.org, kbernhisel@precisiontherapeutics.com Message-ID: <6218769.30833.1276039183117.JavaMail.mshaeffer@127.0.0.1> Content-Type: text/plain; charset=UTF-8; format=flowed; delsp=no Amy Senn asked: Now that Oncotech is out of business, who is everyone sending their extreme resistance assays to? We were just briefed today by a company called Precision Therapeutics out of Pittsburgh PA that they perform Chemo sensitivity and resistance tests and the test is called ChemoFx. Their website is www.precisiontherapeutics.com. I have no affiliation with this company. Precision Therapeutics supplies the shipping container and fluid, usually overnight delivery with Saturday deliveries allowed. Marc Shaeffer Tucson Medical Center Arizona ------------------------------ Message: 16 Date: Tue, 08 Jun 2010 18:54:11 -0500 (CDT) From: Steven Hacker Subject: Re: RE: [Histonet] block disposal To: jstaruk@masshistology.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <1232490361.717331.1276041251677.JavaMail.root@vms246.mailsrvcs.net> Content-Type: text/plain; charset="UTF-8" Hey, Joyce... Contact some of your vendors...Many of them would love to get addition companies will d compensate/donate to th difference in future assays. Regards, Steven Hacker Ps...Yes, I work for Biocare Medical and we're always interested in bl prefer to kn and if they were po information will ever be accepted.< Jun 8, 2010 01:38:11 PM, jstaruk@masshistology.com wrote: < We are also s returning all blocks back t _______________________ James E. Staruk HT(ASCP)www.masshistology.com www.nehorselabs.com -----Orig From: histonet-bounces@lists.utsouthwestern.edu [ma Sherwood, M Sent: Tuesday, June 08, 2010 11:36 AM To: Joyce Cline; histo Subject: RE: [Histonet] block disposal < We are a research laboratory. Our policy, based on communication via Histonet, is to keep 2 years worth of paraffin blocks in the lab. B greater than 2 years old up to 10 years old, we send to off-site s torage. Blocks greater than 10 years old are destroyed by the storage co mpany since they have them. When we first set up this system last of blocks and the majority were put in our incinerated. Peggy From: histonet-bounces@lists.utsouthwestern.e [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Sent: Tuesday, June 08, 2010 10:28 AM To: histonet@lists.utsou Subject: [Histonet] block disposal How does everyon 10 years old? Are they i company or just thrown in In Maryland I was told they can't be thrown in the regula by a state waste official. Joyce Cline, Technical Special Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 jo ________________________________ ***** CONFIDENT confidential information and i are not the named addre copy this e-mail. Please have received this e-mail ___________________ Histonet mailing list Histonet@lists.uts http://lists.utsouthwestern.edu/mailman/listinfo/histone The information in this e-mail is intended only for the person whom it is addressed. If you believe this e-mail was sent to you in and the e-mail contains patient information, please contact the Pa Compliance HelpLine at http://www.partners.org/complianceline . I e-mail was sent to you in error but does not contain patient infor contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailin Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern. Checked by AVG - www.avg.com Versi 06/08/10 02:3 _______________________________________________ Histonet Histonet@lists.utsouthwestern.edu http://lists.utsouthw ------------------------------ Message: 17 Date: Tue, 8 Jun 2010 19:59:31 -0400 From: Brandi Higgins Subject: Re: [Histonet] Oncotech To: "Senn, Amy R" Cc: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, Many of our surgeons who previously requested we send specimens to Oncotech switched to Precision Therapeutics about a year or so ago (although some still used Oncotech at times). We have used Precision with no problems. They even supplied us with a refrigerator to store the specimen collection kits which need to be refrigerated, and our reps have been very helpful overall. Hope this helps. Brandi Higgins, HT(ASCP) On Mon, Jun 7, 2010 at 9:27 AM, Senn, Amy R wrote: > Hello Histonet, > > > > > > Now that Oncotech is out of business, who is everyone sending their > extreme resistance assays to? > > > > > > Thanks, > > Amy, HT > > Camp Hill, PA > > > > > > > > > > > > Attention: This Message is intended only for the use of the individual or > entity to which it is addressed, and may contain information that is > privileged, confidential and exempt from disclosure under applicable law. If > the reader of this message is not the intended recipient, you are hereby > notified that any dissemination or copying of this message or the taking of > any action in reliance on the contents of this message is strictly > prohibited. If you have received this message in error, please notify us > immediately and destroy the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 18 Date: Wed, 9 Jun 2010 07:07:39 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] Special Stain Procedures To: , Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D838AA@EMAIL.archildrens.org> Content-Type: text/plain; charset="US-ASCII" For copper we use the kit from American Master Tech. It works really well. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com Sent: Tuesday, June 08, 2010 3:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Procedures Does anyone have the following procedures they can share with me: - Rhodamine (for copper) - Victoria Blue Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 19 Date: Wed, 9 Jun 2010 08:21:50 -0400 From: John Shelley Subject: [Histonet] RNA FISN on frozen tissue samples To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Histonetters, I have a huge favor to ask, I have been asked to start up work on frozen tissue samples to perform RNA FISH. I am looking for some individuals how are doing this or attempting to do this and can help shed some light on this undertaking. We successfully were able to get this protocol to work on cultures cells form a few different lines but know that the transition may be much more difficult in human tissue samples. Again any help or advise anyone can give would be greatly appreciated. If you want to contact me offline that would be fine. Thanks in advance for any help. John ------------------------------ Message: 20 Date: Wed, 9 Jun 2010 06:01:44 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Special Stain Procedures To: histonet@lists.utsouthwestern.edu, thisisann@aol.com Message-ID: <929438.23665.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Neither procedure is reliable. Use Tim's method (sulfur dioxide). Ren? J. --- On Tue, 6/8/10, thisisann@aol.com wrote: From: thisisann@aol.com Subject: [Histonet] Special Stain Procedures To: histonet@lists.utsouthwestern.edu Date: Tuesday, June 8, 2010, 4:43 PM Does anyone have the following procedures they can share with me: - Rhodamine (for copper) - Victoria Blue Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 9 Jun 2010 06:08:21 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Refrigerator Mold To: "histonet@lists.utsouthwestern.edu" , Jennifer Anderson Message-ID: <316872.16387.qm@web65706.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You should never use an open container with bleach in any place where you keep slides, because bleach will affect the antigens in the sections and affect histochemical reactions as well. Carboard folders will catch fungus spores outside the refrigerator that eventually will develop into fungi, as in your case. Carboard folders are not a good option to store slides in the humid environment of a refrigerator. You should put your slides in plastic boxes, either in large ones (100 slides each) or small ones (25 slides). You will be able alto to store more slides in less space. Ren? J. ? ? --- On Tue, 6/8/10, Jennifer Anderson wrote: From: Jennifer Anderson Subject: [Histonet] Refrigerator Mold To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, June 8, 2010, 3:34 PM Hello and Greetings. I am looking for a solution to our refrigerator mold problem.? We have stacks of folders in our refrigerators, and they get moldy with time. I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage. I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents. Thanks so much for all of your continued insight and advice! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Wed, 09 Jun 2010 09:41:50 -0400 From: "Richard Cartun" Subject: [Histonet] Explanted prosthetic valves To: "Histonet" Message-ID: <4C0F61DE.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII For those of you who work in hospitals: What is your policy on explanted prosthetic valves? Discard or send to pathology? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 23 Date: Wed, 09 Jun 2010 08:47:09 -0500 From: "Bliven, Laura" Subject: [Histonet] Image Analysis To: "histonet@lists.utsouthwestern.edu" , "ihcrg@googlegroups.com" Message-ID: <201006091347.o59DlBca021717@spamfilt> Content-Type: text/plain; charset="iso-8859-1" What software systems is everyone in clinical settings using for image analysis of markers such as p53, MIB1, Estrogen, Progesterone, HER2 IHC, possibly and HER2 FISH for starters? What characteristics do you like and not like about your system? Thanks for time and feedback. Feedback is so valuable in everyone's job, no matter where you work. Laura Laura Bliven, AAS, HT(ASCP), QIHC Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 8 *************************************** ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA. From Timothy.Morken <@t> ucsfmedctr.org Wed Jun 9 15:08:22 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Jun 9 15:08:33 2010 Subject: [Histonet] CRE: JAHCO vs. CAP In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C08FB7CDE@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C08FB7CDE@mailsvr.MARSHMED.local> Message-ID: <1AAF670737F193429070841C6B2ADD4C018803BEBE@EXMBMCB15.ucsfmedicalcenter.org> Yes, we do that... You pay your money and they send the materials. JC accepts CAP as the proficiency provider. JC is more oriented to the whole system rather than just the lab. JC is not as specific towards lab technical issues as CAP is. They use "tracers" to follow everything done to and for a patient in the system. In the lab that means ensuring your QA/QC system is airtight. They can look at ANYTHING related to a given test - reagent QA, technologist training/proficiency documentation, instrument records, QC documentation, etc. They want to see how well you follow a QA/QC system. No downside, just a bit different than CAP. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, June 09, 2010 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] JAHCO vs. CAP We are having a discussion about switching our inspection process form CAP to JAHCO. One of our questions is, can we still participate in the proficiency testing through CAP> If anyone has done this can you explain the process and the upside and the down side. Thanks Gary M. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DianaRip1 <@t> aol.com Wed Jun 9 16:46:14 2010 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Wed Jun 9 16:46:36 2010 Subject: [Histonet] Control Blocks Message-ID: <60533.10f80689.394165a6@aol.com> I Histonet] Control BlocksKnutson, Deanne _DKnutson <@t> primecare.org _ (mailto:histonet@lists.utsouthwestern.edu?Subject=[Histonet]%20Control%20Block s&In-Reply-To=) Fri Jun 4 12:39:44 CDT 2010 * Previous message: _[Histonet] H&E Stainer Validations _ (http://lists.utsouthwestern.edu/pipermail/histonet/2010-June/051040.html) * Next message: _[Histonet] PARAPLAST QUALITY _ (http://lists.utsouthwestern.edu/pipermail/histonet/2010-June/051038.html) * Messages sorted by: _[ date ]_ (http://lists.utsouthwestern.edu/pipermail/histonet/2010-June/date.html#51037) _[ thread ]_ (http://lists.utsouthwestern.edu/pipermail/histonet/2010-June/thread.html#51037) _[ subject ]_ (http://lists.utsouthwestern.edu/pipermail/histonet/2010-June/subject.html#51037) _[ author ]_ (http://lists.utsouthwestern.edu/pipermail/histonet/2010-June/author.html#51037) ____________________________________ Hello, Does anyone out there have positive Pneumocystis control blocks that they may want to trade with? We have some positive GRAM control blocks that we could exchange. Please let me know. Thank you so much! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 _dknutson <@t> primecare.org_ (http://lists.utsouthwestern.edu/mailman/listinfo/histonet) primecare.org_ (http://lists.utsouthwestern.edu/mailman/listinfo/histonet) > ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. I would love to trade some Pneumocystis control blocks for some positive Gram Controls. How many do you need? From tahseen <@t> brain.net.pk Wed Jun 9 23:53:22 2010 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Wed Jun 9 23:53:26 2010 Subject: [Histonet] Ocular fiative Message-ID: <24371.202.125.145.178.1276145602.squirrel@brain.net.pk> HI all, We are using Alcoholic formalin fixative for ocular (Eye) specimens, Reference Tech Sample (ASCP) HT-2(1995), since last 15 year without facing any problem. Now one of our pathologists (New joiner) wants to fix Ocular in 10%NBF. I would lick to know how are doing others, and is there any comparison between 10%NBF verses Alcoholic fixative for Ocular (Eye). Thanks, Muhammad Tahseen Histology Supervisor SKMCH&RC Lahore Pakistan From Farnana <@t> nehealth.com Thu Jun 10 06:42:04 2010 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Thu Jun 10 06:42:11 2010 Subject: [Histonet] ink on film coverslips Message-ID: <4C109755.26ED.00D9.1@nehealth.com> Hi everyone, I am trying to completely remove the dotting ink from our slides. I have tried alcohol and it takes off the blue dotting ink but the green ink leaves a yellow faded dot on the cover slip. The cover slips that we use are the film coverslip so I think the ink gets absorbed somehow. Do you have any ideas that may take the ink off of the cover slips? I would like to avoid taking the coverslip off the slide if possible. Thank you, Amy Farnan Northeast Health Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From DKBoyd <@t> chs.net Thu Jun 10 07:18:25 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Jun 10 07:18:34 2010 Subject: [Histonet] JAHCO vs. CAP In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C08FB7CDE@mailsvr.MARSHMED.local> Message-ID: We switched from CAP to TJC about 3 years ago. Proficiency is the same. Order from CAP per usual. TJC recognizes CAP as an accrediting agency and peer. I have not seen a down side. I have on the other hand seen an up side to the TJC Lab inspection. You are no longer being inspected by your peers, who sometimes lose perspective of the regulations and try to make you a mirror image of there institute. Or you may get inspectors who have just gone through a bad inspection and take it out on you. I have seen this done before. When we were CAP and inspected hospitals, our group always tried to make it a learning experience for both parties. I just had my 2nd TJC inspection in March. The inspector was wonderful, very knowledgeable and more than welling to share. Best inspection I've ever had. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Martin, Gary" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/09/2010 03:44 PM To cc Subject [Histonet] JAHCO vs. CAP We are having a discussion about switching our inspection process form CAP to JAHCO. One of our questions is, can we still participate in the proficiency testing through CAP> If anyone has done this can you explain the process and the upside and the down side. Thanks Gary M. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From kmerriam2003 <@t> yahoo.com Thu Jun 10 07:57:15 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Jun 10 07:57:18 2010 Subject: [Histonet] Histodeck Flash Cards In-Reply-To: <69B641EF1B5F431B89D9D368A7CA5E40.MAI@accuwebhosting.biz> References: <69B641EF1B5F431B89D9D368A7CA5E40.MAI@accuwebhosting.biz> Message-ID: <38990.97006.qm@web50303.mail.re2.yahoo.com> Hi Laurie, I purchased them because I am thinking about taking the HTL in the next year or so.? The are pretty nice, they are divided up into categories (fixation, processing, etc) and each category has a different color edge on the card.? They?have?questions on the front?with the answers and explanations on the back.??They were pretty expensive (75-80, I think), but would probably help out a lot when studying for an exam (a non-histologist could ask the questions and help you study)!? I will probably enlist my 9 year old, who gets a kick out of this kind of thing. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "laurie@conxis.com" To: histonet@lists.utsouthwestern.edu Sent: Tue, June 8, 2010 11:20:46 AM Subject: [Histonet] Histodeck Flash Cards Hi Everyone, I was wondering if anyone out there has used the Histodeck flash cards to study for the HTL exam?? Just curious. Thanks, Laurie *********************** From sgoebel <@t> xbiotech.com Thu Jun 10 08:34:53 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Thu Jun 10 08:34:58 2010 Subject: [Histonet] Ocular fiative Message-ID: <20100610063453.9e2d9aa830e8449a2412eb1e4f2f067e.fb7de4e547.wbe@email04.secureserver.net> Davidson?s Fixative 740 mL 37% Formaldehyde 1110 mL 40 mL Glacial Acetic Acid 1110 mL Tap Water I have used this recipe for some time and it works re Maybe this would satisfy your pathologist? YOu can p fresh into this and fix overnight, then process routinely.& sections turn out great!! Sarah Goeb Histotechnicia XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100< Austin, Texas 78744 < (512)386-5107 -------- Original Message -------- Subject: [Histonet] Ocular fiative From: [1]tahseen@brain.net.pk Date: Wed, June 09, 2010 9:53 pm To: [2]histonet@lists.u HI all, We are using Alcoholic formalin fixative for ocular (Eye) specimens, Reference Tech Sample (ASCP) HT-2(1995), since last 15 year without facing< Now one of our pathologists (New joiner) wants to fix Ocular in 10%NBF. I would lick to know how are doing others, and is there any comparison between 10%NBF verses Alcoholic fixative for Ocular (Eye). Thanks, Muhammad Tahseen Histology Supervisor SKMCH&RC Lahore Pakistan _______________________________________________ Histonet mailing list [3]Histonet@lists.utsou [4]http: References 1. 3D"mailto://tahseen@brain.net.pk"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Lynn.Burton <@t> Illinois.gov Thu Jun 10 11:26:52 2010 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Jun 10 11:27:27 2010 Subject: [Histonet] Histodeck Flash Cards References: <69B641EF1B5F431B89D9D368A7CA5E40.MAI@accuwebhosting.biz> <38990.97006.qm@web50303.mail.re2.yahoo.com> Message-ID: Where did you purchase them? Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kim Merriam Sent: Thu 6/10/2010 7:57 AM To: laurie@conxis.com Cc: Histonet Subject: Re: [Histonet] Histodeck Flash Cards Hi Laurie, I purchased them because I am thinking about taking the HTL in the next year or so. The are pretty nice, they are divided up into categories (fixation, processing, etc) and each category has a different color edge on the card. They have questions on the front with the answers and explanations on the back. They were pretty expensive (75-80, I think), but would probably help out a lot when studying for an exam (a non-histologist could ask the questions and help you study)! I will probably enlist my 9 year old, who gets a kick out of this kind of thing. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "laurie@conxis.com" To: histonet@lists.utsouthwestern.edu Sent: Tue, June 8, 2010 11:20:46 AM Subject: [Histonet] Histodeck Flash Cards Hi Everyone, I was wondering if anyone out there has used the Histodeck flash cards to study for the HTL exam? Just curious. Thanks, Laurie *********************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From djohnson <@t> mercedesmedical.com Thu Jun 10 12:12:11 2010 From: djohnson <@t> mercedesmedical.com (Dave Johnson) Date: Thu Jun 10 12:13:00 2010 Subject: [Histonet] Histodeck Flash Cards In-Reply-To: References: <69B641EF1B5F431B89D9D368A7CA5E40.MAI@accuwebhosting.biz><38990.97006.qm@web50303.mail.re2.yahoo.com> Message-ID: http://www.ascp.org/ASCPStore/Store/Books/5930.aspx Authors: Freida L. Carson and Christa Hladik Designed for quick and easy self-study for the ASCP HTL certifying examination, HistoDeck flash cards include questions structured like those on the exam. The cards cover content based on the new third edition of Freida Carson's classic Histotechnology volume and include additional images not included in the book. If you use flash cards for exam prep, this is the study tool for you. Contents: Fixation - 38 questions, 13 images Immunohistochemistry - 30 questions, 6 images Instrumentation - 20 questions, 15 images Processing - 19 questions, 10 images Routine staining - 33 questions, 17 images Safety - 32 questions, 5 images Special staining - 74 questions, 39 images Miscellaneous - 34 questions, 6 images 400 cards * 111 images * 2009 ISBN: 9780891895930 * Order # 5930 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Thursday, June 10, 2010 12:27 PM To: Kim Merriam; laurie@conxis.com Cc: Histonet Subject: RE: [Histonet] Histodeck Flash Cards Where did you purchase them? Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kim Merriam Sent: Thu 6/10/2010 7:57 AM To: laurie@conxis.com Cc: Histonet Subject: Re: [Histonet] Histodeck Flash Cards Hi Laurie, I purchased them because I am thinking about taking the HTL in the next year or so. The are pretty nice, they are divided up into categories (fixation, processing, etc) and each category has a different color edge on the card. They have questions on the front with the answers and explanations on the back. They were pretty expensive (75-80, I think), but would probably help out a lot when studying for an exam (a non-histologist could ask the questions and help you study)! I will probably enlist my 9 year old, who gets a kick out of this kind of thing. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "laurie@conxis.com" To: histonet@lists.utsouthwestern.edu Sent: Tue, June 8, 2010 11:20:46 AM Subject: [Histonet] Histodeck Flash Cards Hi Everyone, I was wondering if anyone out there has used the Histodeck flash cards to study for the HTL exam? Just curious. Thanks, Laurie *********************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From integrated.histo <@t> gmail.com Thu Jun 10 12:48:41 2010 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Thu Jun 10 12:48:45 2010 Subject: [Histonet] Thin Prep Message-ID: Our lab is currently looking for another Thin Prep machine. Does anyone know where I can locate a used model? Cindy DuBois Integrated Pathology Stockton, CA From rgrow <@t> bmnet.com Thu Jun 10 14:56:30 2010 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Thu Jun 10 14:56:33 2010 Subject: [Histonet] Full Time HT in E. Tennessee Message-ID: Blount Memorial Hospital in Maryville TN has a full time histology technician position open Monday thru Friday. We have good benefits, with possible relocation assistance for the right candidate. If you are interested in working for a hospital that cares about its patients, we may be what you are looking for. We have a new (3yrs) histology laboratory, excellent ventilation, and ample workspace. We do approximately 12,000 cases/yr routine small and large surgical?s, & biopsies with frozen, routine H&E, special stains, and about 33 IHC antibodies (automated). The processing room is separate with 3 tissue processors, 2 run nightly, with one for same-day turn-around cases. General histology experience with certification preferred. Some Immunohistochemistry would be helpful. Weekdays 7-3:30pm with occasional variance in schedule as needed. Must demonstrate competency and successfully complete the on-the-job orientation through the histology section of the laboratory. Perform all duties of a Histology Technician and other duties as assigned. Applicants must meet the educational and training requirements necessary for certification by the American Society of Clinical Pathology as a Histology Technician or have experience equal to certification. We are located just 20 minutes from the beautiful Smoky Mountain National Park and experience 4 wonderful seasons! All types of outdoor activities are possible. Maryville is host to the annual Foothills Fall Festival with top name entertainment and crafts, and just 30 minutes from Knoxville's cultural events and entertainment, as well as UT football, basketball, etc. Gatlinburg and Pigeon Forge are close too! Anyone interested please visit our website at blountmemorial.org. to fill out an application and attach a resume. Thanks, Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. From saby_joseph_a <@t> yahoo.com Thu Jun 10 17:05:04 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Thu Jun 10 17:05:07 2010 Subject: [Histonet] Ocular fiative In-Reply-To: <24371.202.125.145.178.1276145602.squirrel@brain.net.pk> References: <24371.202.125.145.178.1276145602.squirrel@brain.net.pk> Message-ID: <179259.91336.qm@web114418.mail.gq1.yahoo.com> Tahseen- I work in the world of animal/research histology.? I have spent many years working with rodent and nonrodent eyes, developing my skills and knowledge.? Different fixatives work well with different parts of the eye.? 10% NBF might wotk well for cornea, bulbar and palpebral conjunctiva.? But it is just about the worst fixative for retina.? Most glutaraldehyde fixatives are hypertonic and cause the eyes to collaspe unless "windows" (~2-5 mm holes cut along lateral or medial margins of the eye to allow fixative to penetrate the vitreous humour) are cut in the eyes ~1-5 hours after they go into fix.? But glut is very good for most eye structures, although it tends to overharden the lens.? The very best retina fixation I have ever seen is with (real) Zenker's fixative.? The Zn Zenker substitutes I have seen are terrible for this application. For?most eye structures, Davidson's can work well.? Overfixing (more than 24 hours) can cause tissue swelling and overhardening.? But in a hospital setting, this may be just what would be needed.? There is more than one recipe for Davidson's I've seen over the years, some having been altered to better fix testes.? Check with the books to make sure you have the original recipe. It will be interesting to hear what our hospital colleagues use for eyes.? I would guess most use 10% NBF.? And I am sure their pathologists say that this is just fine. Good luck! Joe Saby, BA HT ? ________________________________ From: "tahseen@brain.net.pk" To: histonet@lists.utsouthwestern.edu Sent: Thu, June 10, 2010 12:53:22 AM Subject: [Histonet] Ocular fiative HI all, We are using Alcoholic formalin fixative for ocular (Eye) specimens, Reference Tech Sample (ASCP) HT-2(1995), since last 15 year without facing any problem. Now one of our pathologists (New joiner) wants to fix Ocular in 10%NBF. I would lick to know how are doing others, and is there any comparison between 10%NBF verses Alcoholic fixative for Ocular (Eye). Thanks, Muhammad Tahseen Histology Supervisor SKMCH&RC Lahore Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kryan <@t> nfderm.com Fri Jun 11 10:18:31 2010 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Fri Jun 11 10:18:39 2010 Subject: [Histonet] Job Opening Message-ID: Hi Everyone, We are a derm lab located in beautiful Jacksonville, Florida. We are looking for a full time histologist with at least one year experience. Must be ASCP certified and/or have a current Florida technologist license. Shift will be Monday - Friday, 6:00 am - 2:30 p.m. Great working atmosphere with a wonderful small team. Duties to include routine histology, IHC, and an occasional special stain. Competitive salary and good benefits. If interested, please contact me by e-mail or Gloria Thompson (HR) at 904-398-0547, ext 1106 for more information. Have a great day, Kaye Ryan Histology Supervisor From jpwiner <@t> gmail.com Fri Jun 11 10:45:14 2010 From: jpwiner <@t> gmail.com (jessamine winer) Date: Fri Jun 11 10:45:23 2010 Subject: [Histonet] Can you do Masson's trichrome on frozen sections Message-ID: Hi, We normally do trichrome on paraffin sections but I was wondering if it's possible to do on frozen sections. I don't want to waste my time if it is just going destroy the tissue. If it will work what thickness sections do I need, what fixative and are there any modifications I need to make to the standard protocol. Thanks, Jessamine From pruegg <@t> ihctech.net Fri Jun 11 10:53:58 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jun 11 10:54:49 2010 Subject: SPAM-LOW: [Histonet] ink on film coverslips In-Reply-To: <4C109755.26ED.00D9.1@nehealth.com> References: <4C109755.26ED.00D9.1@nehealth.com> Message-ID: Try acetone which will also remove the tape cs, maybe that is what you should do, remove the tape coverslip in acetone and recover it. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Farnan Sent: Thursday, June 10, 2010 5:42 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] ink on film coverslips Hi everyone, I am trying to completely remove the dotting ink from our slides. I have tried alcohol and it takes off the blue dotting ink but the green ink leaves a yellow faded dot on the cover slip. The cover slips that we use are the film coverslip so I think the ink gets absorbed somehow. Do you have any ideas that may take the ink off of the cover slips? I would like to avoid taking the coverslip off the slide if possible. Thank you, Amy Farnan Northeast Health Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thoward <@t> unm.edu Fri Jun 11 12:42:59 2010 From: thoward <@t> unm.edu (Tamara A Howard) Date: Fri Jun 11 12:43:05 2010 Subject: [Histonet] Definiens Tissue Studio? Message-ID: Apologies to everyone on the cross-post for this question, but I'm looking for feedback on this image analysis package (Definiens Tissue Studio). One of our faculty members is interested in it & I know nothing about it, other than what the company website has to offer; I'd rather hear from people who actually use it! Their group looks at a variety of sample types - whole mounts, cultured cells, & sections by both fluorescence (epi & confocal) and brightfield microscopy. Pros, cons, any insights will be appreciated. Feel free to reply off-line; I won't post any replies I receive in confidence. Thanks! Tamara *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** From Brenda <@t> nsh.org Fri Jun 11 12:57:38 2010 From: Brenda <@t> nsh.org (Brenda Royce) Date: Fri Jun 11 12:57:46 2010 Subject: [Histonet] RE: Histonet Digest, Vol 79, Issue 11 In-Reply-To: References: Message-ID: NSH has the Histodeck Flash Cards available for sale. Visit our website https://www.nshonline.org/eweb/DynamicPage.aspx?expires=yes&webcode=COEPubSearch to purchase. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, June 11, 2010 1:25 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Histodeck Flash Cards (Dave Johnson) 2. Thin Prep (Cindy DuBois) 3. Full Time HT in E. Tennessee (rgrow@bmnet.com) 4. Re: Ocular fiative (Joseph Saby) 5. Job Opening (Kaye Ryan) 6. Can you do Masson's trichrome on frozen sections (jessamine winer) 7. RE: SPAM-LOW: [Histonet] ink on film coverslips (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Thu, 10 Jun 2010 13:12:11 -0400 From: "Dave Johnson" Subject: RE: [Histonet] Histodeck Flash Cards To: "Burton, Lynn" , "Kim Merriam" , Cc: Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" http://www.ascp.org/ASCPStore/Store/Books/5930.aspx Authors: Freida L. Carson and Christa Hladik Designed for quick and easy self-study for the ASCP HTL certifying examination, HistoDeck flash cards include questions structured like those on the exam. The cards cover content based on the new third edition of Freida Carson's classic Histotechnology volume and include additional images not included in the book. If you use flash cards for exam prep, this is the study tool for you. Contents: Fixation - 38 questions, 13 images Immunohistochemistry - 30 questions, 6 images Instrumentation - 20 questions, 15 images Processing - 19 questions, 10 images Routine staining - 33 questions, 17 images Safety - 32 questions, 5 images Special staining - 74 questions, 39 images Miscellaneous - 34 questions, 6 images 400 cards * 111 images * 2009 ISBN: 9780891895930 * Order # 5930 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Thursday, June 10, 2010 12:27 PM To: Kim Merriam; laurie@conxis.com Cc: Histonet Subject: RE: [Histonet] Histodeck Flash Cards Where did you purchase them? Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kim Merriam Sent: Thu 6/10/2010 7:57 AM To: laurie@conxis.com Cc: Histonet Subject: Re: [Histonet] Histodeck Flash Cards Hi Laurie, I purchased them because I am thinking about taking the HTL in the next year or so. The are pretty nice, they are divided up into categories (fixation, processing, etc) and each category has a different color edge on the card. They have questions on the front with the answers and explanations on the back. They were pretty expensive (75-80, I think), but would probably help out a lot when studying for an exam (a non-histologist could ask the questions and help you study)! I will probably enlist my 9 year old, who gets a kick out of this kind of thing. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "laurie@conxis.com" To: histonet@lists.utsouthwestern.edu Sent: Tue, June 8, 2010 11:20:46 AM Subject: [Histonet] Histodeck Flash Cards Hi Everyone, I was wondering if anyone out there has used the Histodeck flash cards to study for the HTL exam? Just curious. Thanks, Laurie *********************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 10 Jun 2010 10:48:41 -0700 From: Cindy DuBois Subject: [Histonet] Thin Prep To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Our lab is currently looking for another Thin Prep machine. Does anyone know where I can locate a used model? Cindy DuBois Integrated Pathology Stockton, CA ------------------------------ Message: 3 Date: Thu, 10 Jun 2010 15:56:30 -0400 From: rgrow@bmnet.com Subject: [Histonet] Full Time HT in E. Tennessee To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Blount Memorial Hospital in Maryville TN has a full time histology technician position open Monday thru Friday. We have good benefits, with possible relocation assistance for the right candidate. If you are interested in working for a hospital that cares about its patients, we may be what you are looking for. We have a new (3yrs) histology laboratory, excellent ventilation, and ample workspace. We do approximately 12,000 cases/yr routine small and large surgical???s, & biopsies with frozen, routine H&E, special stains, and about 33 IHC antibodies (automated). The processing room is separate with 3 tissue processors, 2 run nightly, with one for same-day turn-around cases. General histology experience with certification preferred. Some Immunohistochemistry would be helpful. Weekdays 7-3:30pm with occasional variance in schedule as needed. Must demonstrate competency and successfully complete the on-the-job orientation through the histology section of the laboratory. Perform all duties of a Histology Technician and other duties as assigned. Applicants must meet the educational and training requirements necessary for certification by the American Society of Clinical Pathology as a Histology Technician or have experience equal to certification. We are located just 20 minutes from the beautiful Smoky Mountain National Park and experience 4 wonderful seasons! All types of outdoor activities are possible. Maryville is host to the annual Foothills Fall Festival with top name entertainment and crafts, and just 30 minutes from Knoxville's cultural events and entertainment, as well as UT football, basketball, etc. Gatlinburg and Pigeon Forge are close too! Anyone interested please visit our website at blountmemorial.org. to fill out an application and attach a resume. Thanks, Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. ------------------------------ Message: 4 Date: Thu, 10 Jun 2010 15:05:04 -0700 (PDT) From: Joseph Saby Subject: Re: [Histonet] Ocular fiative To: tahseen@brain.net.pk, histonet@lists.utsouthwestern.edu Message-ID: <179259.91336.qm@web114418.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Tahseen- I work in the world of animal/research histology.? I have spent many years working with rodent and nonrodent eyes, developing my skills and knowledge.? Different fixatives work well with different parts of the eye.? 10% NBF might wotk well for cornea, bulbar and palpebral conjunctiva.? But it is just about the worst fixative for retina.? Most glutaraldehyde fixatives are hypertonic and cause the eyes to collaspe unless "windows" (~2-5 mm holes cut along lateral or medial margins of the eye to allow fixative to penetrate the vitreous humour) are cut in the eyes ~1-5 hours after they go into fix.? But glut is very good for most eye structures, although it tends to overharden the lens.? The very best retina fixation I have ever seen is with (real) Zenker's fixative.? The Zn Zenker substitutes I have seen are terrible for this application. For?most eye structures, Davidson's can work well.? Overfixing (more than 24 hours) can cause tissue swelling and overhardening.? But in a hospital setting, this may be just what would be needed.? There is more than one recipe for Davidson's I've seen over the years, some having been altered to better fix testes.? Check with the books to make sure you have the original recipe. It will be interesting to hear what our hospital colleagues use for eyes.? I would guess most use 10% NBF.? And I am sure their pathologists say that this is just fine. Good luck! Joe Saby, BA HT ? ________________________________ From: "tahseen@brain.net.pk" To: histonet@lists.utsouthwestern.edu Sent: Thu, June 10, 2010 12:53:22 AM Subject: [Histonet] Ocular fiative HI all, We are using Alcoholic formalin fixative for ocular (Eye) specimens, Reference Tech Sample (ASCP) HT-2(1995), since last 15 year without facing any problem. Now one of our pathologists (New joiner) wants to fix Ocular in 10%NBF. I would lick to know how are doing others, and is there any comparison between 10%NBF verses Alcoholic fixative for Ocular (Eye). Thanks, Muhammad Tahseen Histology Supervisor SKMCH&RC Lahore Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 11 Jun 2010 11:18:31 -0400 From: "Kaye Ryan" Subject: [Histonet] Job Opening To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Everyone, We are a derm lab located in beautiful Jacksonville, Florida. We are looking for a full time histologist with at least one year experience. Must be ASCP certified and/or have a current Florida technologist license. Shift will be Monday - Friday, 6:00 am - 2:30 p.m. Great working atmosphere with a wonderful small team. Duties to include routine histology, IHC, and an occasional special stain. Competitive salary and good benefits. If interested, please contact me by e-mail or Gloria Thompson (HR) at 904-398-0547, ext 1106 for more information. Have a great day, Kaye Ryan Histology Supervisor ------------------------------ Message: 6 Date: Fri, 11 Jun 2010 08:45:14 -0700 From: jessamine winer Subject: [Histonet] Can you do Masson's trichrome on frozen sections To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, We normally do trichrome on paraffin sections but I was wondering if it's possible to do on frozen sections. I don't want to waste my time if it is just going destroy the tissue. If it will work what thickness sections do I need, what fixative and are there any modifications I need to make to the standard protocol. Thanks, Jessamine ------------------------------ Message: 7 Date: Fri, 11 Jun 2010 09:53:58 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] ink on film coverslips To: "'Amy Farnan'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Try acetone which will also remove the tape cs, maybe that is what you should do, remove the tape coverslip in acetone and recover it. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Farnan Sent: Thursday, June 10, 2010 5:42 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] ink on film coverslips Hi everyone, I am trying to completely remove the dotting ink from our slides. I have tried alcohol and it takes off the blue dotting ink but the green ink leaves a yellow faded dot on the cover slip. The cover slips that we use are the film coverslip so I think the ink gets absorbed somehow. Do you have any ideas that may take the ink off of the cover slips? I would like to avoid taking the coverslip off the slide if possible. Thank you, Amy Farnan Northeast Health Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 11 **************************************** From ratliffjack <@t> hotmail.com Fri Jun 11 13:04:02 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Jun 11 13:05:28 2010 Subject: [Histonet] NSH Hard Tissue Forum - August 14th, 2010 in Philadelphia, PA Message-ID: The Hard Tissue Committee of the National Society for Histotechnology is proud to present a one day Hard Tissue Forum. This first of its kind event will be held Saturday, August 14th, 2010 in Philadelphia, PA, from 8:00 am to 5:00 pm at the Doubletree Hotel Philadelphia. Join us as we seek to further our knowledge and understanding of the histology and analysis of bone and how this information can better serve in the diagnosis of bone related diseases and the efficacy and safety of therapeutic treatments. Topics include: Bone Biology 101; In Vivo Models for Musculoskeletal Research; Concepts for Specimen Management of Samples for Decalcified Paraffin Processing; Resin Histology ? A Practical Approach for Demonstrating Undemineralized Bone; Skeletal Analysis with MicroCT ? What You Need to Know and Why You Need To Know It; and Bone Histomorphometry. Don?t miss out on this one time compilation of information solely dedicated to bone as presented from both scientists and histotechnologists actively working in the field. This forum will also be a perfect opportunity to network with professionals within the ?hard tissue niche?. We hope to see you in August! Click Here for More Information and To Download a Copy of The Registration Brochure or contact Jack L Ratliff, NSH Hard Tissue Committee Chair @ 317-281-1975 or jratliff@biomimetics.com. Best Regards, Jack Ratliff NSH-Hard Tissue Committe Chair _________________________________________________________________ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2 From jphistology <@t> gmail.com Fri Jun 11 13:11:54 2010 From: jphistology <@t> gmail.com (Joao Pessoa) Date: Fri Jun 11 13:12:10 2010 Subject: [Histonet] Cell culture materials needed Message-ID: Does anyone have some cell culture that they are willing to part ways with? Preferably this would be from a human source, but the particular cell line and/or characteristics are not important to my research. I know from my own experiences that during passaging (AKA subculture or splitting cells) materials are often discarded- I would gladly pay for the shipping of such material. Ideally I would like some material already processed and FFPE, but I can do that part myself as well. Please let me know if you can help me out! I am happy to answer questions. Thanks! Joao Histo Tech From nancy_schmitt <@t> pa-ucl.com Fri Jun 11 13:35:10 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Jun 11 13:35:25 2010 Subject: [Histonet] congo red Message-ID: <737BD0BF52F0744B96B74B61756AC0644166B9BE89@hestia.ad.pa-ucl.com> TGIF!! We are having a bit of a problem with Congo Red control coming off; we keep the block refrigerated and reseal after each use. The patient specimen does not seem to be an issue and both are cut at 6. We do not cut control slides ahead of time. Any thoughts would be appreciated. These are run on the Artisan. Thanks in advance for your help- Nancy Schmitt HT(ASCP), MLT(CSMLS) United Clinic Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From rjbuesa <@t> yahoo.com Fri Jun 11 14:05:32 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 11 14:05:35 2010 Subject: [Histonet] Can you do Masson's trichrome on frozen sections In-Reply-To: Message-ID: <639985.57381.qm@web65706.mail.ac4.yahoo.com> Although I have NOT try doing Masson on FS, I think the sections would not survive (that is why I have not try it). You could, and share your results with all of us. Ren? J. --- On Fri, 6/11/10, jessamine winer wrote: From: jessamine winer Subject: [Histonet] Can you do Masson's trichrome on frozen sections To: histonet@lists.utsouthwestern.edu Date: Friday, June 11, 2010, 11:45 AM Hi, We normally do trichrome on paraffin sections but I was wondering if it's possible to do on frozen sections.? I don't want to waste my time if it is just going destroy the tissue.? If it will work what thickness sections do I need, what fixative and are there any modifications I need to make to the standard protocol. Thanks, Jessamine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From youngir <@t> gmail.com Fri Jun 11 22:02:22 2010 From: youngir <@t> gmail.com (Ian Young) Date: Fri Jun 11 22:02:28 2010 Subject: [Histonet] HistoBob.com Message-ID: Dear Histonetters, I would like to take this opportunity to tell you all about a project I have been working on with a couple of colleagues of mine. I had been frustrated while trying to gain information pertaining to our field. Reading text books took to long and frankly couldn't hold my attention. Conferences became too expensive for our lab to send a tech too every year so I never got to go. Journals also were too expensive and the vocabulary was so clinical that it needed a cryptologist to decipher. From this frustration came an idea. Why can't there be a website that has all of the available information in one place? On that fateful day, when the frustration became to great, HistoBob.com was born. Written by histotechs, for histotechs, HistoBob.com is a place where anyone can find new information about Histotechnology. Conferences, new technology, polices and regulations, nothing is off limits. Histobob.com is not just information, but social and professional networking. Check out our forums and find other histotechs with the same questions you have. Just did something really cool in your lab? Start a thread and tell us about it. Histobob.com also gives you the ability to comment on any article we post, so if you really don't' agree with something we stated or we made a mistake, you can let us know. Of course if you really like the article, we would love to hear that to. Your feedback will drive what content goes up on Histobob.com. Are you on Twitter or Facebook? So are we! Every time we update the website, a tweet goes out automatically letting you know what's new. Follow our link on the site to go directly to our Twitter account or our Facebook page. What about when you are not by a computer and you need your late night histo fix? We've got you covered. The Histobob.com podcast will be starting soon, covering a wide range of information and topics, it might make you laugh as well. We hope you like it, because we made it for all of us Histotechs out there. All we ask is that if you like, spread the word!! Please to enjoy.... Histobob.com Ian Young, HTL(ASCP) Creator, Editor-in-Chief Histobob.com From rsrichmond <@t> gmail.com Sat Jun 12 12:59:58 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Jun 12 13:00:02 2010 Subject: [Histonet] Re: Can you do Masson's trichrome on frozen sections Message-ID: The Engel-Cunningham variant of the Gomori trichrome stain was developed for skeletal muscle frozen sections, and is a fairly simple one-step trichrome stain once you get it working. I've never used it for any other tissue. I think it's commercially available in kit form. Bob Richmond Samurai Pathologist Knoxville TN From Ken_Marissael <@t> vwr.com Sat Jun 12 13:01:13 2010 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Sat Jun 12 13:01:17 2010 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 06/12/2010 and will not return until 06/13/2010 From Jaclyn_Bhamornsiri <@t> vwr.com Sat Jun 12 13:01:14 2010 From: Jaclyn_Bhamornsiri <@t> vwr.com (Jaclyn_Bhamornsiri@vwr.com) Date: Sat Jun 12 13:01:19 2010 Subject: [Histonet] Jaclyn Bhamornsiri is out of the office Message-ID: I will be out of the office starting 06/11/2010 and will not return until 06/12/2010 From classicdoc <@t> gmail.com Sun Jun 13 12:13:29 2010 From: classicdoc <@t> gmail.com (Douglas Gregg) Date: Sun Jun 13 12:13:37 2010 Subject: [Histonet] Need a microtome Message-ID: I am starting a collaboration with a small start-up company on a very exciting cancer therapy. They want me to set up a small histo lab and do some pro bono IHC. I am willing but they need a microtome. I am looking for a decent working microtome with a disposable blade holder. Does anyone have one laying around in a closet that would sell it to a good cause. Thanks, Doug Gregg Veterinary pathologist retired from Plum Island Animal Disease Center Southold, NY 11971 From rjbuesa <@t> yahoo.com Sun Jun 13 12:59:00 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jun 13 12:59:04 2010 Subject: [Histonet] Need a microtome In-Reply-To: Message-ID: <435457.95994.qm@web65701.mail.ac4.yahoo.com> Try eBay. They usually have microtomes a very good prices. Ren? J. --- On Sun, 6/13/10, Douglas Gregg wrote: From: Douglas Gregg Subject: [Histonet] Need a microtome To: histonet@lists.utsouthwestern.edu Date: Sunday, June 13, 2010, 1:13 PM I am starting a collaboration with a small start-up company on a very exciting? cancer therapy. They want me to set up a small histo lab and do some pro bono IHC. I am willing but they need a microtome. I am looking for a decent working microtome with a disposable blade holder. Does anyone have one laying around in a closet that would sell it? to a good cause. Thanks, Doug Gregg Veterinary pathologist retired from Plum Island Animal Disease Center Southold, NY 11971 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Mon Jun 14 09:46:31 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Mon Jun 14 09:46:56 2010 Subject: [Histonet] thin prep Message-ID: Hologic may have a few [1]www.hologic.com Thanks Pathology Supervis Kathy Baldwin, SCT (ASCP) Memorial Hospital&nb [2]sbaldwin@mhhcc.org Ph 812-482-0210, Pager 812-481-089 Confidential information, Authorized use only. References 1. 3D"http://www.hologic.com"/ 2. 3D"mailto:sbaldwin@mhhc From loftonjt <@t> holycrosshealth.org Mon Jun 14 10:19:40 2010 From: loftonjt <@t> holycrosshealth.org (Jimmy Lofton) Date: Mon Jun 14 10:19:58 2010 Subject: [Histonet] On leave Message-ID: <4C16104C.9B70.0056.0@holycrosshealth.org> I will be on leave from June 15 to June 21. Thanks, Jimmy Lofton, M.S., HT,CT(ASCP) Manager Histology Laboratory Holy Cross Hospital 1500 Forest Glen Road Silver Spring, MD 20910-1484 301-754-7353 (Phone) 301-754-8563 (Fax) loftonjt@holycrosshealth.org Trinity Health MailGate made the following annotations --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message. --------------------------------------------------------------------- From shive003 <@t> umn.edu Mon Jun 14 11:21:13 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Jun 14 11:21:18 2010 Subject: [Histonet] Porcine Hemagglutinating Encephalomyelitis Virus IHC Message-ID: Does anyone know of a commercial source for unconjugated anti-porcine hemagglutinating encephalomyelitis virus that can be used on FFPE tissues? Thanks in advance, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) From cbrya <@t> lexclin.com Mon Jun 14 11:33:55 2010 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Mon Jun 14 11:34:01 2010 Subject: [Histonet] bar-coding Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF0595E96336@EXCHANGESB> Does anyone have SoftPath 4.2? If so, do you know if it has the capability to produce bar-coded specimen labels? I am looking into various methods to reduce re-entry of case data and eliminate handwriting of cassettes and slides. I know Ventana's Vantage system can do this for histology, but we also have cytology processing in our lab. I would like to find something that would be uniform for both cytology and histology processing and interface with SoftPath. However, if SoftPath could do this, even better. Thank you in advance for any input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From cbarone <@t> NEMOURS.ORG Mon Jun 14 11:46:45 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon Jun 14 11:46:48 2010 Subject: [Histonet] RE: Histonet Digest, Vol 79, Issue 13 In-Reply-To: References: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7D97@wlmmsx01.nemours.org> We use a modified Gomori's one step....with excellent results on frozen muscle. You can find the protocol in Muscle Pathology and Histochemistry, Harvey Sarnat. It is an excellent stain...and if you have a limited amount of tissue, it gives you the most information on a single slide....(and if the tissue was quickly... and properly frozen....will include fiber typing). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, June 13, 2010 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Can you do Masson's trichrome on frozen sections (Robert Richmond) 2. Ken Marissael is out of the office (Ken_Marissael@vwr.com) 3. Jaclyn Bhamornsiri is out of the office (Jaclyn_Bhamornsiri@vwr.com) ---------------------------------------------------------------------- Message: 1 Date: Sat, 12 Jun 2010 13:59:58 -0400 From: Robert Richmond Subject: [Histonet] Re: Can you do Masson's trichrome on frozen sections To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 The Engel-Cunningham variant of the Gomori trichrome stain was developed for skeletal muscle frozen sections, and is a fairly simple one-step trichrome stain once you get it working. I've never used it for any other tissue. I think it's commercially available in kit form. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 2 Date: Sat, 12 Jun 2010 14:01:13 -0400 From: Ken_Marissael@vwr.com Subject: [Histonet] Ken Marissael is out of the office To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 06/12/2010 and will not return until 06/13/2010 ------------------------------ Message: 3 Date: Sat, 12 Jun 2010 14:01:14 -0400 From: Jaclyn_Bhamornsiri@vwr.com Subject: [Histonet] Jaclyn Bhamornsiri is out of the office To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 06/11/2010 and will not return until 06/12/2010 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 13 **************************************** From tbritten <@t> aol.com Mon Jun 14 12:28:29 2010 From: tbritten <@t> aol.com (Thomas Britten) Date: Mon Jun 14 12:29:07 2010 Subject: [Histonet] Porcine Hemagglutinating Encephalomyelitis Virus IHC In-Reply-To: References: Message-ID: <8D607429-70F6-45D5-A21A-29E62E7602EB@aol.com> What is this virus. Sent from my iPhone On Jun 14, 2010, at 12:21 PM, "Jan Shivers" wrote: > Does anyone know of a commercial source for unconjugated anti- > porcine hemagglutinating encephalomyelitis virus that can be used on > FFPE tissues? > > Thanks in advance, > Jan Shivers > Senior Scientist > Histology/IHC/EM Section Head > Pathology Teaching Program > University of Minnesota > Veterinary Diagnostic Laboratory > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > > (Confidentiality Notice: This message, together with any > attachments, is intended only for the use of the individual or > entity to which it is addressed and may contain confidential or > privileged information. If you think you have received this message > in error, please advise the sender and then delete this message and > any attachments immediately.) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michaels <@t> janelia.hhmi.org Mon Jun 14 12:54:35 2010 From: michaels <@t> janelia.hhmi.org (Susan Michael) Date: Mon Jun 14 12:54:39 2010 Subject: [Histonet] Special Stainer for Sale Message-ID: We have a Leica ST5020 special stainer that has barely been used, and we are looking to sell it. Please let me know if this is something you may be interested in. Susan Michael, HTL (ASCP) Histology/Anatomy Janelia Farms Research Campus 19700 Helix Drive Ashburn, VA 20147 From joyceagain <@t> att.net Mon Jun 14 13:06:51 2010 From: joyceagain <@t> att.net (Joyce Bidwell) Date: Mon Jun 14 13:07:00 2010 Subject: [Histonet] Certification Message-ID: <386013.49517.qm@web81107.mail.mud.yahoo.com> I am in Kansas City, MO and I am in the process of getting enrolled with Indiana State University to obtain my HT certificate.? Does anyone out there know if #1.? It is a requirement in Missouri to have either a license or certificate to cut permanent slides?? #2.? Are there any other schools that offer the HT certificate training course that is a distance learning class and enrolls any time other that Fall.? As it stands now I will have my prerequisits done at the end of next fall but will not be able to start the Indiana State University class until the Fall of 2011.? The doctor I am working with now would like me to be able to become certified or "licensed" before then so I can cut permanent slides.? Currently I am cutting MOHS tissue only.? Thanks for your input!! From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jun 14 13:13:26 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jun 14 13:13:43 2010 Subject: [Histonet] Hpylori Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB26AE@HPEMX3.HealthPartners.int> Does anyone have an abundance of Hpylori tissue in blocks they would be willing to part with or trade for another control block? We are running very low and haven't had a positive for a while!! Much appreciated!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From thomas.crowell <@t> novartis.com Mon Jun 14 13:18:44 2010 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Mon Jun 14 13:19:01 2010 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 06/14/2010 and will not return until 06/15/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From brandihiggins <@t> gmail.com Mon Jun 14 14:21:01 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Mon Jun 14 14:21:07 2010 Subject: [Histonet] Certification In-Reply-To: <386013.49517.qm@web81107.mail.mud.yahoo.com> References: <386013.49517.qm@web81107.mail.mud.yahoo.com> Message-ID: Hello, I enrolled in the online HT program through Harford Community College in Maryland. It is completely online. You do have to send in sample slides / blocks / special stains, but you complete all tests and such online through blackboard (which I also used in college, you my be familiar with that). When I enrolled it was a rolling continuous enrollment, and suggested completion time was 10 montsh but it was completely go at your own pace. Hope this helps. Feel free to email me if you have any other questions. You also need to have a certified "mentor", so hopefully your lab has an ASCP certified tech. Brandi Higgins, HT(ASCP) On Mon, Jun 14, 2010 at 2:06 PM, Joyce Bidwell wrote: > I am in Kansas City, MO and I am in the process of getting enrolled with > Indiana State University to obtain my HT certificate. Does anyone out there > know if #1. It is a requirement in Missouri to have either a license or > certificate to cut permanent slides? #2. Are there any other schools that > offer the HT certificate training course that is a distance learning class > and enrolls any time other that Fall. As it stands now I will have my > prerequisits done at the end of next fall but will not be able to start the > Indiana State University class until the Fall of 2011. The doctor I am > working with now would like me to be able to become certified or "licensed" > before then so I can cut permanent slides. Currently I am cutting MOHS > tissue only. > > Thanks for your input!! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shive003 <@t> umn.edu Mon Jun 14 14:25:27 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Jun 14 14:25:36 2010 Subject: [Histonet] Porcine Hemagglutinating Encephalomyelitis Virus IHC References: <8D607429-70F6-45D5-A21A-29E62E7602EB@aol.com> Message-ID: Causal agent - a neurotropic coronavirus of young pigs; characterized by vomiting and wasting disease and/or motor disorders (incoordination, convulsions ) due to acute encephalomyelitis (which can lead to death within days). Pigs are the only known natural host of the virus. Jan Shivers ----- Original Message ----- From: "Thomas Britten" To: "Jan Shivers" Cc: "histonet" Sent: Monday, June 14, 2010 12:28 PM Subject: Re: [Histonet] Porcine Hemagglutinating Encephalomyelitis Virus IHC > What is this virus. > > Sent from my iPhone > > On Jun 14, 2010, at 12:21 PM, "Jan Shivers" wrote: > >> Does anyone know of a commercial source for unconjugated anti- porcine >> hemagglutinating encephalomyelitis virus that can be used on FFPE >> tissues? >> >> Thanks in advance, >> Jan Shivers >> Senior Scientist >> Histology/IHC/EM Section Head >> Pathology Teaching Program >> University of Minnesota >> Veterinary Diagnostic Laboratory >> 1333 Gortner Ave. >> St. Paul, MN 55108 >> 612-624-7297 >> shive003@umn.edu >> >> (Confidentiality Notice: This message, together with any attachments, is >> intended only for the use of the individual or entity to which it is >> addressed and may contain confidential or privileged information. If you >> think you have received this message in error, please advise the sender >> and then delete this message and any attachments immediately.) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sfeher <@t> CMC-NH.ORG Mon Jun 14 16:35:59 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Jun 14 16:36:03 2010 Subject: [Histonet] bar-coding In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF0595E96336@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF0595E96336@EXCHANGESB> Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B753FB@exchange.cmc-nh.org> We have SoftPath 4.3 out print servers use version 4.2. We have Leica's IPC and IPS cassette and slide printers and print all our cassettes and slides (we print directly to the slides and do not use paper labels), including ThinPrep slides for the Imager. Leica's application specialists got with SoftPath and got it all working. We have no issues and are printing slides with 2d barcodes. The bar-coded slides work well on the BondMax IHC stainer and we are working on an interface for the Dako Artisan Link. It's all been working well for us. What kind of slide and cassette printers are you using or considering using? Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Monday, June 14, 2010 12:34 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] bar-coding Does anyone have SoftPath 4.2? If so, do you know if it has the capability to produce bar-coded specimen labels? I am looking into various methods to reduce re-entry of case data and eliminate handwriting of cassettes and slides. I know Ventana's Vantage system can do this for histology, but we also have cytology processing in our lab. I would like to find something that would be uniform for both cytology and histology processing and interface with SoftPath. However, if SoftPath could do this, even better. Thank you in advance for any input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alexia_fran <@t> hotmail.com Mon Jun 14 17:09:31 2010 From: alexia_fran <@t> hotmail.com (=?Windows-1252?B?QWxleGlhIEZyYW5jaXNjYSBO+vFleiBQYXJyYQ==?=) Date: Mon Jun 14 17:09:35 2010 Subject: [Histonet] Uneven fluorescent staining Message-ID: Hello everyone!! The Histonet Archives have been always very helpful to me, so I decided to become a member and ask a personal question to the experts. I am doing NeuN and BrdU double staining in the olfactory bulb, following the protocol described below. I have always had some trouble with the NeuN staining, obtaining sometimes good or no staining at all. I tried to trouble shoot with the intracardial perfusion and even bought a new NeuN antibody (Chemicon). I am having now, however, another problem. I observed an uneven NeuN and BrdU staining. There are some parts of the section that get a really good and bright staining and others that do not get any staining at all (even though they should). Can anyone give a hint on what to review or change? Thank you so much!!! Alexia 1. Intracardial perfusion with cold PBS and cold 4%PFA. 2. The brains are postfixed for 4 hrs in PFA 4% at 4C and immersed in sucrose 30% ON. After that, the brains are embedded in tissue tek and store at -80. 3. Sections of 20um are obtained using a cryostat and collected using positive charged slides. 4. Allow the slides to reach the RT 5. Warm slides at 55C x 10? 6. Outline slides with Immedge pen 7. Hydrate: PBS 2 x 5?, final quick wash with ddH20 8. Soak slides in 2N HCl for 1 hr at 37C 9. Neutralize washing the slides with 0.1M Na tretaborate buffer pH8.5, 3 x 10? 10. Wash with PBS 2 x 10? 11. Wash with PBS-T 1 x 10? 12. Block at RT with 10% of Donkey serum in PBST x 2hrs (60uL on each section) 13. First antibody: incubate at 4C with first antibody diluited in PBST with 2.5% Donkey serum ON (16-24hrs) 14. Wash with PBST 4x10? at RT 15. Secondary antibody: dilute 1:750 alexa antibodies in PBST with 2.5% Donkey serum x 2 hrs at RT 16. Wash with PBST 1 x 10?, and then with PBS 3x 10? 17. Mount with Vectashield and seal using transparent nail polish. _________________________________________________________________ From ratliffjack <@t> hotmail.com Mon Jun 14 23:05:32 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Jun 14 23:05:37 2010 Subject: [Histonet] Hard Tissue Forum - August 14th, 2010 in Philadelphia, PA Message-ID: Attention All Hard Tissue Histologists!!!! The Hard Tissue Committee of the National Society for Histotechnology is proud to present a one day Hard Tissue Forum. This first of its kind event will be held Saturday, August 14th, 2010 in Philadelphia, PA, from 8:00 am to 5:00 pm at the Doubletree Hotel Philadelphia. Join us as we seek to further our knowledge and understanding of the histology and analysis of bone and how this information can better serve in the diagnosis of bone related diseases and the efficacy and safety of therapeutic treatments. Don?t miss out on this one time compilation of information solely dedicated to bone as presented from both scientists and histotechnologists actively working in the field. This forum will also be a perfect opportunity to network with professionals within the ?hard tissue niche?. We hope to see you in August! Check out the NSH website @ www.nsh.org to download a copy of the brochure or feel free to contact Jack L Ratliff, NSH Hard Tissue Committee Chair @ 317-281-1975 or jratliff@biomimetics.com for more information. PROGRAM AT A GLANCE Registration Fees Member: $119 Non Member: $159 Schedule at Glance: 7:30am?8:00am Registration & Contintental Breakfast 8:00am?9:00am Bone Biology 101 Presented by Damien Laudier,BS,HTL(ASCP),QIHC, Laudier Histology, New York, NY 9:00am?10:30am In Vivo Models for Musculoskeletal Research Presented by Timothy Ganey,PhD, Atlanta Medical Center, Atlanta, GA 10:30am?10:45am Refreshment Break 10:45am?12:15pm Concepts for Specimen Management of Samples for Decalcified Paraffin Processing Presented by Robert A. Skinner,HTL(ASCP), University of Arkansas for Medical Sciences 12:15pm?1:15pm Lunch on Your Own 1:15pm?2:45pm Resin Histology: A Practical Approach for Demonstrating Undemineralized Bone Presented by Jack L. Ratliff, BA, BioMimetic Therapeutics, Inc., Franklin, TN 2:45pm?3:00pm Refreshment Break 3:00pm?4:00pm Skeletal Analysis with MicroCT: What You Need To Know And Why You Need To Know It Presented by Daniel S. Perrien, PhD, Vanderbilt Center for Bone Biology, Nashville, TN 4:00pm?5:00pm Bone Histomorphometry Presented by Damien Laudier,BS, HTL(ASCP),QIHC, Laudier Histology, New York, NY Travel Arrangements Lodging Doubletree Hotel Philadelphia 237 S. Broad Street, Philadelphia, PA 19107 Reservations via Phone: 215.893.1668 Registrants are responsible for hotel / travel arrangements. We have secured a small block of rooms at the discounted group rate of $125.00 plus applicable taxes. If you make reservations via phone please inform the agent that you are with the National Society for Histotechnology Group. Hotel Deadline is July 14, 2010. Getting to the Forum For directions, parking rates and airport shuttle information please visit the Doubletree's website. If you are flying the the Forum the Philadelphia International Airport is 9 miles from the hotel. Contact NSH Travel Services for best rate on air fare. Call 877.510.4747 between 8:30am-5:30pm, CT M-F or book on-line at www.nshtvl.com Cancellation/Substitution Requests All cancellations must be received in writing by July 14, 2010 to receive a full refund. Cancellations received after July 14, 2010 and no shows to the forum are non-refundable. Substitutions are accepted at any time. We must receive this request in writing. Individuals registering after July 14, 2010 will not be eligible for a refund. _________________________________________________________________ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2 From Teri.Hallada <@t> midmichigan.org Tue Jun 15 06:55:28 2010 From: Teri.Hallada <@t> midmichigan.org (Teri.Hallada@midmichigan.org) Date: Tue Jun 15 06:55:43 2010 Subject: [Histonet] Antibody Validation Message-ID: <8839B08E3ED7364E8CBBD53882C984D514102B37@MAILSRV01.midmichigan.net> I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation. I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From renafail <@t> bellsouth.net Tue Jun 15 07:56:55 2010 From: renafail <@t> bellsouth.net (Rena Fail) Date: Tue Jun 15 07:56:58 2010 Subject: [Histonet] RE Antibody validation Message-ID: <513753.56918.qm@web180308.mail.gq1.yahoo.com> Teri New lots of Antibodies recieved?by a?lab should be validated?by ?that laboratory.? Thus proving the new lots produce similar results sa the old under the same?set of circumstances.?Processing, ?Retrevial methods, thickness of sections,?techniques etc?used in your laboratory contribute to the outcome. ?????? Having the Ab validated by the vendor does not verify that it works in your laboratory with your procedures. Rena Fail ----- Original Message ---- From: "Teri.Hallada@midmichigan.org" To: histonet@pathology.swmed.edu Sent: Tue, June 15, 2010 7:55:28 AM Subject: [Histonet] Antibody Validation I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation.? I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law.? The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited.? If you have received this email in error, please advise by immediate reply.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brandihiggins <@t> gmail.com Tue Jun 15 08:51:04 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Tue Jun 15 08:51:16 2010 Subject: [Histonet] fume hood Message-ID: Hello, Our hospital is doing some renovation and we need to look into new fume hoods for our new location. Currently we have one fume hood over our grossing area, and one fume hood in our coverslipping area (two different rooms). The hospital wants to put our grossing room and histo/cyto rooms together. I am still going to need two separate hoods. Does anyone have any experience/knowledge/input about fume hoods? I'm trying to look into the ductless ones, although I imagine changing the filters will end up being more expensive over time (I have no idea what would be involved in running a duct/vent). Also I have seen a benchtop downdraft type that sucks the air down, and does not have a top. It is advertised as being good for xylene. Does anyone use this in their coverslipping area? Any input would be greatly appreciated. I'm pretty clueless on the whole issue. I want to make sure that what I get will be safe for me and my coworker as we will be spending most of our day in this room. Any input is appreciated! Thank You! Brandi Higgins, BS, HT(ASCP) From rjbuesa <@t> yahoo.com Tue Jun 15 09:49:34 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 15 09:49:39 2010 Subject: [Histonet] Antibody Validation In-Reply-To: <8839B08E3ED7364E8CBBD53882C984D514102B37@MAILSRV01.midmichigan.net> Message-ID: <99966.29247.qm@web65716.mail.ac4.yahoo.com> Teri: You are right about the validations you propose although I am not surprised that your vendor does not think it is necessary. They are in the business of selling and you are in the business of assuring the high?quality of your work to obtaining the most accurate work for patients' sake. There is where the difference resides. Ignore your vendor and keep validating your protocols. Ren? J. --- On Tue, 6/15/10, Teri.Hallada@midmichigan.org wrote: From: Teri.Hallada@midmichigan.org Subject: [Histonet] Antibody Validation To: histonet@pathology.swmed.edu Date: Tuesday, June 15, 2010, 7:55 AM I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation.? I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law.? The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited.? If you have received this email in error, please advise by immediate reply.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jun 15 09:53:03 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 15 09:53:13 2010 Subject: [Histonet] fume hood In-Reply-To: Message-ID: <422333.48491.qm@web65705.mail.ac4.yahoo.com> Over the counter fumes hood with filters are very effcicient and cheaper than running the vent ducts, especially in a large building. They also have the advantage that they can be moved to another place if necessary. Those without a cover are not that efficient. Ren? J. --- On Tue, 6/15/10, Brandi Higgins wrote: From: Brandi Higgins Subject: [Histonet] fume hood To: histonet@lists.utsouthwestern.edu Date: Tuesday, June 15, 2010, 9:51 AM Hello, Our hospital is doing some renovation and we need to look into new fume hoods for our new location.? Currently we have one fume hood over our grossing area, and one fume hood in our coverslipping area (two different rooms).? The hospital wants to put our grossing room and histo/cyto rooms together.? I am still going to need two separate hoods.? Does anyone have any experience/knowledge/input about fume hoods?? I'm trying to look into the ductless ones, although I imagine changing the filters will end up being more expensive over time (I have no idea what would be involved in running a duct/vent).? Also I have seen a benchtop downdraft type that sucks the air down, and does not have a top.? It is advertised as being good for xylene. Does anyone use this in their coverslipping area?? Any input would be greatly appreciated.? I'm pretty clueless on the whole issue.? I want to make sure that what I get will be safe for me and my coworker as we will be spending most of our day in this room.? Any input is appreciated!? Thank You! Brandi Higgins, BS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Tue Jun 15 10:19:46 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Jun 15 10:20:02 2010 Subject: [Histonet] fume hood In-Reply-To: References: Message-ID: There are two units we use in our labs and I suggest looking into the Mopec BF600 Downdraft Workcell. This works great for H&E/FS stain lines and other areas w/ open containers of chemicals. I also suggest considering the Labconco Protector XVS Ventilation Station for counter top hood with easy access, the unit is connected to outside air extraction. There are many other options and you should do a search on the internet for more options. William DeSalvo, B.S., HTL(ASCP) Production Manager, Sonora Quest laboratories Chair, NSH QCC > Date: Tue, 15 Jun 2010 09:51:04 -0400 > From: brandihiggins@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] fume hood > > Hello, > > Our hospital is doing some renovation and we need to look into new fume > hoods for our new location. Currently we have one fume hood over our > grossing area, and one fume hood in our coverslipping area (two different > rooms). The hospital wants to put our grossing room and histo/cyto rooms > together. I am still going to need two separate hoods. Does anyone have > any experience/knowledge/input about fume hoods? I'm trying to look into > the ductless ones, although I imagine changing the filters will end up being > more expensive over time (I have no idea what would be involved in running a > duct/vent). Also I have seen a benchtop downdraft type that sucks the air > down, and does not have a top. It is advertised as being good for xylene. > Does anyone use this in their coverslipping area? Any input would be > greatly appreciated. I'm pretty clueless on the whole issue. I want to > make sure that what I get will be safe for me and my coworker as we will be > spending most of our day in this room. Any input is appreciated! Thank > You! > > Brandi Higgins, BS, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3 From alexia_fran <@t> hotmail.com Tue Jun 15 10:28:42 2010 From: alexia_fran <@t> hotmail.com (=?iso-8859-1?B?QWxleGlhIEZyYW5jaXNjYSBO+vFleiBQYXJyYQ==?=) Date: Tue Jun 15 10:29:01 2010 Subject: [Histonet] Uneven fluorescent staining In-Reply-To: References: , Message-ID: Thank you so much for your answer Anatoli! - I tried the citrate buffer boiling system and I obtained the same unreliable results with NeuN. Some animals showed a great NeuN staining and others did not. I really thought at that point that my perfusion was the problem and I switched from making my own PFA4% to buying a 16%PFA from Electron Microscopy Sciences, which I think solved the problem. Now I'm obtaining a good NeuN staining but only in some regions of the tissue. - I am using the Rat monoclonal from Abcam (1:250) - I'll consider the new method using Edu labeling for new experiment and will try the ProLong Gold antifade, thank you very much for the advice. Alexia Nunez University of Maryland, College Park > Subject: RE: [Histonet] Uneven fluorescent staining > Date: Tue, 15 Jun 2010 10:26:32 -0400 > From: AGleiberman@cbiolabs.com > To: alexia_fran@hotmail.com > > Alexia, > 1. HCl treatment could be sometimes problematic - many epitopes are quite sensitive to it, so your NeuN antibody will work not in optimal conditions. You can replace acid treatment with citrate buffer boiling - the same treatment that is used for paraffin sections. It will denature DNA as effectively as HCl treatment. > 2. You did not mention what antibody you are using for BrdU - the best are rat monoclonal from Abcam (much better than any mouse monoclonal or sheep or goat polyclonal I have tested) - and you can easy combine them with mouse monoclonal against NeuN (I believe, only mouse anti-NeuN exist). > 3. For future experiments I recommend switch from BrdU labeling to EdU labeling (Click-iT? EdU Alexa Fluor? 488 HCS Assay from Invitrogen) - this technique is more sensitive for studying DNA synthesis than BrdU, staining is faster (30min), does not involve neither denaturation step nor antibody and can be easily combined with any other marker staining. To do antigen staining after EdU visualization, that is simple chemical reaction, you need wash thoroughly your slides and perform your antibody staining. Additional benefit is - much better structure of nuclei because denaturation step sometimes affects them. In addition, because of high sensitivity you can decrease concentration of nucleotide substitute (EdU) 5-10 times in comparison with BrdU that can be crucial for long-term experiments because all these nucleotide analogues (especially BrdU) can affect cell fate in long run. > 4. Finally, I recommend to replace Vectashield with ProLong Gold antifade (Invitrogen) with or without DAPI. It is better for fluorochrome preservation and it will harden overnight - you don't need any nail polish > > Anatoli Gleiberman, PhD > Director of Histopathology > Cleveland Biolabs, Inc > 73 High Street > Buffalo, NY 14203 > phone:716-849-6810 ext.354 > fax:716-849-6817 > e-mail: AGleiberman@cbiolabs.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alexia Francisca N??ez Parra > Sent: Monday, June 14, 2010 6:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Uneven fluorescent staining > > > Hello everyone!! > The Histonet Archives have been always very helpful to me, so I decided to become a member and ask a personal question to the experts. > > I am doing NeuN and BrdU double staining in the olfactory bulb, following the protocol described below. I have always had some trouble with the NeuN staining, obtaining sometimes good or no staining at all. I tried to trouble shoot with the intracardial perfusion and even bought a new NeuN antibody (Chemicon). I am having now, however, another problem. I observed an uneven NeuN and BrdU staining. There are some parts of the section that get a really good and bright staining and others that do not get any staining at all (even though they should). > > Can anyone give a hint on what to review or change? > > Thank you so much!!! > > Alexia > > 1. Intracardial perfusion with cold PBS and cold 4%PFA. > 2. The brains are postfixed for 4 hrs in PFA 4% at 4C and immersed in sucrose 30% ON. After that, the brains are embedded in tissue tek and store at -80. > 3. Sections of 20um are obtained using a cryostat and collected using positive charged slides. > > > > > > > > > > > > > > > 4. > Allow the slides to reach the RT > > > 5. > Warm slides at 55C x 10' > > 6. > Outline slides with Immedge pen > > 7. > Hydrate: PBS 2 x 5', final quick wash with ddH20 > > 8. > Soak slides in 2N HCl for 1 hr at 37C > > 9. > Neutralize washing the slides with 0.1M Na tretaborate buffer > pH8.5, 3 x 10' > > 10. > Wash with PBS 2 x 10' > > 11. > Wash with PBS-T 1 x 10' > > > > 12. > Block at RT with 10% of Donkey serum in PBST x 2hrs (60uL on each section) > > 13. > First antibody: incubate at 4C with first antibody diluited in > PBST with 2.5% Donkey serum ON (16-24hrs) > > 14. > Wash with PBST 4x10' at RT > > 15. > Secondary antibody: dilute 1:750 alexa antibodies in PBST with > 2.5% Donkey serum x 2 hrs at RT > > 16. > Wash with PBST 1 x 10', and then with PBS 3x 10' > > > > 17. > Mount with Vectashield and seal using transparent nail polish. > > > > > > _________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _________________________________________________________________ From BSullivan <@t> shorememorial.org Tue Jun 15 10:49:28 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Tue Jun 15 10:51:44 2010 Subject: [Histonet] Antibody Validation In-Reply-To: <99966.29247.qm@web65716.mail.ac4.yahoo.com> Message-ID: I agree with Rene. All lot to lot and new antibodies need to be checked for consistency. This is part of your validation process. This even holds true for pre-dilutes which are already tested by the manufacturer for optimum results. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Rene J Buesa To Sent by: histonet@pathology.swmed.edu, histonet-bounces@ Teri.Hallada@midmichigan.org lists.utsouthwest cc ern.edu Subject Re: [Histonet] Antibody Validation 06/15/2010 10:49 AM Teri: You are right about the validations you propose although I am not surprised that your vendor does not think it is necessary. They are in the business of selling and you are in the business of assuring the high?quality of your work to obtaining the most accurate work for patients' sake. There is where the difference resides. Ignore your vendor and keep validating your protocols. Ren? J. --- On Tue, 6/15/10, Teri.Hallada@midmichigan.org wrote: From: Teri.Hallada@midmichigan.org Subject: [Histonet] Antibody Validation To: histonet@pathology.swmed.edu Date: Tuesday, June 15, 2010, 7:55 AM I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation.? I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law.? The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited.? If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Tue Jun 15 10:57:21 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Jun 15 10:57:16 2010 Subject: [Histonet] Antibody Validation In-Reply-To: <99966.29247.qm@web65716.mail.ac4.yahoo.com> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C63F5@EXCHANGECLUSTER.yumaregional.local> There are other issue that you have to take into consideration with both instruments, With digital image analysis you also have to look at the questions that CAP just instituted for this. This includes the machine being run by someone that can do high complexity testing. This means that you have to have a tech do this machine, not a TA or Lab aid. There are also issue with consistent calibration of the Image analysis machines, continued education of the techs, and validating not only anitbodies but continued validation of the image algorythms.. Do not listen to vendors, rely on your instinct and validate. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From liz <@t> premierlab.com Tue Jun 15 11:01:16 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Jun 15 11:01:39 2010 Subject: [Histonet] Antibody Validation - long response In-Reply-To: <99966.29247.qm@web65716.mail.ac4.yahoo.com> Message-ID: Bottom line it's not the vendors responsibility to validate their equipment or antibodies in your lab. Some vendors may help you do this, but ultimately the lab needs to validate the equipment and IHC in their lab. The vendors normally calibrate the equipment prior to shipment and once they set the instrument up in your lab, they should be able to provide you with the documentation that states that they calibrated the instrument. Your instruments need to be calibrated prior to being validated. As far as your scanner goes some vendors can provide validation, but it's at a cost and that cost is not cheap depending upon what you actually want validated. If you are using the scanner and associated algorithms for analysis then you need to validate that separately. There are several steps required to validate a scanner - 1. you validate the scanner 2. if you are using a database to store your images then that also may need to be validated and 3. if you are using algorithms that provide you with data then those algorithms need to be validated. For example prior to running a validation protocol on a tissue processor its needs to be calibrated for temperature. All of your major equipment needs to be on a calibration schedule. We calibrate all of our instruments once a year and validation is completed only once unless we change the instrument location or how we use the instrument. Pipettors are calibrated every 6 months. All instruments are validated it may just be a one pager for the basic lab equipment but instruments like the tissue processor, slide staining, IHC stainer and scanner require written protocols some of these are 80 pages in length and go into great detail. The same goes for your antibodies. Antibodies are validated initially with 25 tissue samples (10 strongly positive tissues, 10 moderate to weakly positive tissues and 5 tissues that have no reactivity) This type of validation is required for routine antibodies, prognostic markers such as Her-2, ER and PR require additional tissue samples. New lots require 3 tissue samples one strongly positive on moderate to weakly positive and one negative. If you change the antibody source or detection system or retrieval it needs to be validated again - This information comes from the paper Standarization of Immunohistochemistry from CAP its available on line - I have a copy if you need it. There are also new guidelines for ER/PR and a new article on validation of ER/PR in the June issue of Archives of pathology from CAP. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, June 15, 2010 8:50 AM To: histonet@pathology.swmed.edu; Teri.Hallada@midmichigan.org Subject: Re: [Histonet] Antibody Validation Teri: You are right about the validations you propose although I am not surprised that your vendor does not think it is necessary. They are in the business of selling and you are in the business of assuring the high?quality of your work to obtaining the most accurate work for patients' sake. There is where the difference resides. Ignore your vendor and keep validating your protocols. Ren? J. --- On Tue, 6/15/10, Teri.Hallada@midmichigan.org wrote: From: Teri.Hallada@midmichigan.org Subject: [Histonet] Antibody Validation To: histonet@pathology.swmed.edu Date: Tuesday, June 15, 2010, 7:55 AM I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation.? I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law.? The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited.? If you have received this email in error, please advise by immediate reply.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Tue Jun 15 11:37:04 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Jun 15 11:37:38 2010 Subject: [Histonet] fume hood In-Reply-To: <422333.48491.qm@web65705.mail.ac4.yahoo.com> References: <422333.48491.qm@web65705.mail.ac4.yahoo.com> Message-ID: <4C1773F0.59CD.00EE.0@hurleymc.com> Hi Brandi, Three years ago, our lab was totally renovated. Clinical and anatomic pathology have become one big room. The only rooms that are separate are the grossing room (who sports a nifty Mopec 600 grossing w/vented hood system) and the TB lab. There has been some adjusting for the employees during this time. The med techs could not tolerate our xylene under any circumstances. Just changing the solutions in the processor/stainers was a whole new experience for them, much less any minor spill. In fact, having a minor spill ended up closing the lab for service, but that is another story. Switching over to xlyene substitute has worked out well after making adjustments. It sounds like just you and cytology are combined, so I hope that cytology has considered a hood for their non-gyn prep as you just never know about contaminates. Our cytology area has a great biohazard floor model and is also vented. In histology, we have a back draft built right into the back of the countertops that helps a lot with our manual special stains, and our automated coverslipper (era 1994) has a cover over it and is tied into the same venting system as the grossing station. In retrospect, the only changes I wish I would have thought of, was to make sure that the entire venting system has a dedicated electrical hook-up. Because when the electricity goes down (and it will due to weather or a frisky squirrel), people cannot work in the fumes. It's amazing how quickly it gets bad. At first you don't realize it and then everyone starts getting xylene/formalin side affects. Not pretty. That's all I can think of and sorry it was long winded! Try and get the best for you and your people. Filters are expensive and in my opinion, don't give as good air exchange and a vented system. Best regards, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 >>> Rene J Buesa 6/15/2010 10:53 AM >>> Over the counter fumes hood with filters are very effcicient and cheaper than running the vent ducts, especially in a large building. They also have the advantage that they can be moved to another place if necessary. Those without a cover are not that efficient. Ren? J. --- On Tue, 6/15/10, Brandi Higgins wrote: From: Brandi Higgins Subject: [Histonet] fume hood To: histonet@lists.utsouthwestern.edu Date: Tuesday, June 15, 2010, 9:51 AM Hello, Our hospital is doing some renovation and we need to look into new fume hoods for our new location. Currently we have one fume hood over our grossing area, and one fume hood in our coverslipping area (two different rooms). The hospital wants to put our grossing room and histo/cyto rooms together. I am still going to need two separate hoods. Does anyone have any experience/knowledge/input about fume hoods? I'm trying to look into the ductless ones, although I imagine changing the filters will end up being more expensive over time (I have no idea what would be involved in running a duct/vent). Also I have seen a benchtop downdraft type that sucks the air down, and does not have a top. It is advertised as being good for xylene. Does anyone use this in their coverslipping area? Any input would be greatly appreciated. I'm pretty clueless on the whole issue. I want to make sure that what I get will be safe for me and my coworker as we will be spending most of our day in this room. Any input is appreciated! Thank You! Brandi Higgins, BS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WBENTON <@t> umm.edu Tue Jun 15 11:49:25 2010 From: WBENTON <@t> umm.edu (Walter Benton) Date: Tue Jun 15 11:49:34 2010 Subject: [Histonet] Fume hood In-Reply-To: References: Message-ID: <4C1776D5020000F40005B815@GWIA2.umm.edu> Brandi, I have been through several renovations and highly recommend that you get a hood that exhaust outside. Depending upon what applications you plan to carry out under the hood, filters may not do the trick. It is also important to have someone from your facilities or engineering department conduct an air exchange analysis to make sure that you have proper airflow and exchanges within the room, since the two operations are being combined. I would also recommend that you have your area monitored for air quality while performing the various tasks that you perform now in you current configuration to see if you are OSHA compliant or whatever regulatory body you wish to follow. Downdraft ventilation is great for Xylene, because Xylene vapor is heavier than air, thus the reason those systems work well. Feel free to reach out if you have any other questions. Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 >>> On 6/15/2010 at 12:31 PM, wrote: Message: 13 Date: Tue, 15 Jun 2010 09:51:04 -0400 From: Brandi Higgins Subject: [Histonet] fume hood To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, Our hospital is doing some renovation and we need to look into new fume hoods for our new location. Currently we have one fume hood over our grossing area, and one fume hood in our coverslipping area (two different rooms). The hospital wants to put our grossing room and histo/cyto rooms together. I am still going to need two separate hoods. Does anyone have any experience/knowledge/input about fume hoods? I'm trying to look into the ductless ones, although I imagine changing the filters will end up being more expensive over time (I have no idea what would be involved in running a duct/vent). Also I have seen a benchtop downdraft type that sucks the air down, and does not have a top. It is advertised as being good for xylene. Does anyone use this in their coverslipping area? Any input would be greatly appreciated. I'm pretty clueless on the whole issue. I want to make sure that what I get will be safe for me and my coworker as we will be spending most of our day in this room. Any input is appreciated! Thank You! Brandi Higgins, BS, HT(ASCP) This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From maggie.allen <@t> nicewareintl.com Tue Jun 15 12:05:54 2010 From: maggie.allen <@t> nicewareintl.com (Maggie Allen) Date: Tue Jun 15 12:06:10 2010 Subject: [Histonet] RE: bar-coding In-Reply-To: <20100615164854.0DA6CE80A3@spamfilter2.redanvil.net> References: <20100615164854.0DA6CE80A3@spamfilter2.redanvil.net> Message-ID: <6BCD4D0894AE0A4A96B4CB8F6779A57001874A09@NICEWARE-MAIN03.NicewareIntl03.local> Hello Carol, Here is some information for you in regards to printing from your LIS. Please contact me at your convenience to discuss you needs in greater detail. Niceware Healthcare offers patient safety and identification software and services to the healthcare market that are easy to deploy, affordable and fully compliant with current bar code and RFID labeling requirements. Niceware Healthcare provides high value and low maintenance software solutions to meet patient safety goals for patient identification. The NiceForm module provides very specific identification application options in laboratory environments. For example, NiceForm drives histology cassettes and slide printing systems to uniquely identify specimen in laboratories. NiceWatch adds a separate integration layer connecting NiceLabel applications to your Laboratory Information System (LIS). While NiceForm is used for very specific identification applications, NiceWatch adds a bar code to any specimen requisition form when the LIS is incapable to output the required bar code. The solution for Laboratory Specimen Identification provides the following benefits: * Simplified data input and reduced error rate in data processing and label printing * Bar code and RFID smart label printing from any Laboratory Information System (LIS) * Easy connectivity and HL7 global messaging from multiple healthcare information systems * High value but moderately priced http://healthcare.nicewareintl.com/cgi-bin/site.pl?3208&dwContent_contentID=77 If you are interested in going one step further than bar-code printing and would like to learn more about LabelClinic HTS for tracking, verification and validation feel free to register for our free Webinar on June 22nd and 2:00 PM. https://nicewareintl.webex.com/mw0306lb/mywebex/default.do?service=1&siteurl=nicewareintl&nomenu=true&main_url=%2Fmc0805lb%2Fe.do%3Fsiteurl%3Dnicewareintl%26AT%3DMI%26EventID%3D118014662%26UID%3D1048843472%26Host%3D2e9af6872b3d7a5f4059%26RG%3D1%26FrameSet%3D2 Thank you, Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel (810) 629-3930 Cell (215) 200-0268 Corporate Numbers : General: (262) 784-2456 Toll Free: (888) 894-NICE (6423) Fax: (262) 784-2495 Technical Support: (262) 784-2466 Email: maggie.allen@nicewareintl.com www.nicewareintl.com http://healthcare.nicewareintl.com Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel? (810) 629-3930 Cell (215) 200-0268 Corporate Numbers : General: (262) 784-2456 Toll Free: (888) 894-NICE (6423) Fax: (262) 784-2495 Technical Support: (262) 784-2466 Email: maggie.allen@nicewareintl.com www.nicewareintl.com http://healthcare.nicewareintl.com FREE Webinars : June 11, 2010, 10:30AM CST - 11:30AM CST Integrated Printing Using NiceWatch Enterprise Business Connector Register Today! June 18, 2010 - 10:30AM CST - 11:30AM CST NiceLabel Products - A Step above the Rest Register Today! June 25, 2010 - 10:30AM CST - 11:30AM CST NiceForm Training Session Register Today! "The information in this e-mail and any attachments is confidential and may be subject to legal professional privilege. It is intended solely for the attention and use of the named addressee(s). If you are not the intended recipient or person responsible for delivering this information to the intended recipient, please notify the sender immediately. Unless you are the intended recipient or his/her representative you are not authorized to, and must not, read, copy, distribute, use or retain this message or any part of it" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, June 15, 2010 12:49 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Porcine Hemagglutinating Encephalomyelitis Virus IHC (Thomas Britten) 2. Special Stainer for Sale (Susan Michael) 3. Certification (Joyce Bidwell) 4. Hpylori (Webb, Dorothy L) 5. Thomas Crowell is out of the office. (thomas.crowell@novartis.com) 6. Re: Certification (Brandi Higgins) 7. Re: Porcine Hemagglutinating Encephalomyelitis Virus IHC (Jan Shivers) 8. RE: bar-coding (Feher, Stephen) 9. Uneven fluorescent staining (Alexia Francisca N??ez Parra) 10. Hard Tissue Forum - August 14th, 2010 in Philadelphia, PA (Jack Ratliff) 11. Antibody Validation (Teri.Hallada@midmichigan.org) 12. RE Antibody validation (Rena Fail) 13. fume hood (Brandi Higgins) 14. Re: Antibody Validation (Rene J Buesa) 15. Re: fume hood (Rene J Buesa) 16. RE: fume hood (WILLIAM DESALVO) 17. RE: Uneven fluorescent staining (Alexia Francisca N??ez Parra) 18. Re: Antibody Validation (BSullivan@shorememorial.org) 19. RE: Antibody Validation (Jesus Ellin) 20. RE: Antibody Validation - long response (Liz Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Mon, 14 Jun 2010 13:28:29 -0400 From: Thomas Britten Subject: Re: [Histonet] Porcine Hemagglutinating Encephalomyelitis Virus IHC To: Jan Shivers Cc: histonet Message-ID: <8D607429-70F6-45D5-A21A-29E62E7602EB@aol.com> Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes What is this virus. Sent from my iPhone On Jun 14, 2010, at 12:21 PM, "Jan Shivers" wrote: > Does anyone know of a commercial source for unconjugated anti- > porcine hemagglutinating encephalomyelitis virus that can be used on > FFPE tissues? > > Thanks in advance, > Jan Shivers > Senior Scientist > Histology/IHC/EM Section Head > Pathology Teaching Program > University of Minnesota > Veterinary Diagnostic Laboratory > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > > (Confidentiality Notice: This message, together with any > attachments, is intended only for the use of the individual or > entity to which it is addressed and may contain confidential or > privileged information. If you think you have received this message > in error, please advise the sender and then delete this message and > any attachments immediately.) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 14 Jun 2010 13:54:35 -0400 From: Susan Michael Subject: [Histonet] Special Stainer for Sale To: Message-ID: Content-Type: text/plain; charset="US-ASCII" We have a Leica ST5020 special stainer that has barely been used, and we are looking to sell it. Please let me know if this is something you may be interested in. Susan Michael, HTL (ASCP) Histology/Anatomy Janelia Farms Research Campus 19700 Helix Drive Ashburn, VA 20147 ------------------------------ Message: 3 Date: Mon, 14 Jun 2010 11:06:51 -0700 (PDT) From: Joyce Bidwell Subject: [Histonet] Certification To: histonet@lists.utsouthwestern.edu Message-ID: <386013.49517.qm@web81107.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I am in Kansas City, MO and I am in the process of getting enrolled with Indiana State University to obtain my HT certificate.? Does anyone out there know if #1.? It is a requirement in Missouri to have either a license or certificate to cut permanent slides?? #2.? Are there any other schools that offer the HT certificate training course that is a distance learning class and enrolls any time other that Fall.? As it stands now I will have my prerequisits done at the end of next fall but will not be able to start the Indiana State University class until the Fall of 2011.? The doctor I am working with now would like me to be able to become certified or "licensed" before then so I can cut permanent slides.? Currently I am cutting MOHS tissue only.? Thanks for your input!! ------------------------------ Message: 4 Date: Mon, 14 Jun 2010 13:13:26 -0500 From: "Webb, Dorothy L" Subject: [Histonet] Hpylori To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB26AE@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Does anyone have an abundance of Hpylori tissue in blocks they would be willing to part with or trade for another control block? We are running very low and haven't had a positive for a while!! Much appreciated!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 5 Date: Mon, 14 Jun 2010 14:18:44 -0400 From: thomas.crowell@novartis.com Subject: [Histonet] Thomas Crowell is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 06/14/2010 and will not return until 06/15/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. ------------------------------ Message: 6 Date: Mon, 14 Jun 2010 15:21:01 -0400 From: Brandi Higgins Subject: Re: [Histonet] Certification To: Joyce Bidwell , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, I enrolled in the online HT program through Harford Community College in Maryland. It is completely online. You do have to send in sample slides / blocks / special stains, but you complete all tests and such online through blackboard (which I also used in college, you my be familiar with that). When I enrolled it was a rolling continuous enrollment, and suggested completion time was 10 montsh but it was completely go at your own pace. Hope this helps. Feel free to email me if you have any other questions. You also need to have a certified "mentor", so hopefully your lab has an ASCP certified tech. Brandi Higgins, HT(ASCP) On Mon, Jun 14, 2010 at 2:06 PM, Joyce Bidwell wrote: > I am in Kansas City, MO and I am in the process of getting enrolled with > Indiana State University to obtain my HT certificate. Does anyone out there > know if #1. It is a requirement in Missouri to have either a license or > certificate to cut permanent slides? #2. Are there any other schools that > offer the HT certificate training course that is a distance learning class > and enrolls any time other that Fall. As it stands now I will have my > prerequisits done at the end of next fall but will not be able to start the > Indiana State University class until the Fall of 2011. The doctor I am > working with now would like me to be able to become certified or "licensed" > before then so I can cut permanent slides. Currently I am cutting MOHS > tissue only. > > Thanks for your input!! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Mon, 14 Jun 2010 14:25:27 -0500 From: "Jan Shivers" Subject: Re: [Histonet] Porcine Hemagglutinating Encephalomyelitis Virus IHC To: "Thomas Britten" Cc: histonet Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=response Causal agent - a neurotropic coronavirus of young pigs; characterized by vomiting and wasting disease and/or motor disorders (incoordination, convulsions ) due to acute encephalomyelitis (which can lead to death within days). Pigs are the only known natural host of the virus. Jan Shivers ----- Original Message ----- From: "Thomas Britten" To: "Jan Shivers" Cc: "histonet" Sent: Monday, June 14, 2010 12:28 PM Subject: Re: [Histonet] Porcine Hemagglutinating Encephalomyelitis Virus IHC > What is this virus. > > Sent from my iPhone > > On Jun 14, 2010, at 12:21 PM, "Jan Shivers" wrote: > >> Does anyone know of a commercial source for unconjugated anti- porcine >> hemagglutinating encephalomyelitis virus that can be used on FFPE >> tissues? >> >> Thanks in advance, >> Jan Shivers >> Senior Scientist >> Histology/IHC/EM Section Head >> Pathology Teaching Program >> University of Minnesota >> Veterinary Diagnostic Laboratory >> 1333 Gortner Ave. >> St. Paul, MN 55108 >> 612-624-7297 >> shive003@umn.edu >> >> (Confidentiality Notice: This message, together with any attachments, is >> intended only for the use of the individual or entity to which it is >> addressed and may contain confidential or privileged information. If you >> think you have received this message in error, please advise the sender >> and then delete this message and any attachments immediately.) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Mon, 14 Jun 2010 17:35:59 -0400 From: "Feher, Stephen" Subject: RE: [Histonet] bar-coding To: "Carol Bryant" , "Histonet@lists.utsouthwestern.edu" Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B753FB@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" We have SoftPath 4.3 out print servers use version 4.2. We have Leica's IPC and IPS cassette and slide printers and print all our cassettes and slides (we print directly to the slides and do not use paper labels), including ThinPrep slides for the Imager. Leica's application specialists got with SoftPath and got it all working. We have no issues and are printing slides with 2d barcodes. The bar-coded slides work well on the BondMax IHC stainer and we are working on an interface for the Dako Artisan Link. It's all been working well for us. What kind of slide and cassette printers are you using or considering using? Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Monday, June 14, 2010 12:34 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] bar-coding Does anyone have SoftPath 4.2? If so, do you know if it has the capability to produce bar-coded specimen labels? I am looking into various methods to reduce re-entry of case data and eliminate handwriting of cassettes and slides. I know Ventana's Vantage system can do this for histology, but we also have cytology processing in our lab. I would like to find something that would be uniform for both cytology and histology processing and interface with SoftPath. However, if SoftPath could do this, even better. Thank you in advance for any input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 14 Jun 2010 22:09:31 +0000 From: Alexia Francisca N??ez Parra Subject: [Histonet] Uneven fluorescent staining To: Message-ID: Content-Type: text/plain; charset="Windows-1252" Hello everyone!! The Histonet Archives have been always very helpful to me, so I decided to become a member and ask a personal question to the experts. I am doing NeuN and BrdU double staining in the olfactory bulb, following the protocol described below. I have always had some trouble with the NeuN staining, obtaining sometimes good or no staining at all. I tried to trouble shoot with the intracardial perfusion and even bought a new NeuN antibody (Chemicon). I am having now, however, another problem. I observed an uneven NeuN and BrdU staining. There are some parts of the section that get a really good and bright staining and others that do not get any staining at all (even though they should). Can anyone give a hint on what to review or change? Thank you so much!!! Alexia 1. Intracardial perfusion with cold PBS and cold 4%PFA. 2. The brains are postfixed for 4 hrs in PFA 4% at 4C and immersed in sucrose 30% ON. After that, the brains are embedded in tissue tek and store at -80. 3. Sections of 20um are obtained using a cryostat and collected using positive charged slides. 4. Allow the slides to reach the RT 5. Warm slides at 55C x 10' 6. Outline slides with Immedge pen 7. Hydrate: PBS 2 x 5', final quick wash with ddH20 8. Soak slides in 2N HCl for 1 hr at 37C 9. Neutralize washing the slides with 0.1M Na tretaborate buffer pH8.5, 3 x 10' 10. Wash with PBS 2 x 10' 11. Wash with PBS-T 1 x 10' 12. Block at RT with 10% of Donkey serum in PBST x 2hrs (60uL on each section) 13. First antibody: incubate at 4C with first antibody diluited in PBST with 2.5% Donkey serum ON (16-24hrs) 14. Wash with PBST 4x10' at RT 15. Secondary antibody: dilute 1:750 alexa antibodies in PBST with 2.5% Donkey serum x 2 hrs at RT 16. Wash with PBST 1 x 10', and then with PBS 3x 10' 17. Mount with Vectashield and seal using transparent nail polish. _________________________________________________________________ ------------------------------ Message: 10 Date: Tue, 15 Jun 2010 00:05:32 -0400 From: Jack Ratliff Subject: [Histonet] Hard Tissue Forum - August 14th, 2010 in Philadelphia, PA To: Histonet Message-ID: Content-Type: text/plain; charset="Windows-1252" Attention All Hard Tissue Histologists!!!! The Hard Tissue Committee of the National Society for Histotechnology is proud to present a one day Hard Tissue Forum. This first of its kind event will be held Saturday, August 14th, 2010 in Philadelphia, PA, from 8:00 am to 5:00 pm at the Doubletree Hotel Philadelphia. Join us as we seek to further our knowledge and understanding of the histology and analysis of bone and how this information can better serve in the diagnosis of bone related diseases and the efficacy and safety of therapeutic treatments. Don't miss out on this one time compilation of information solely dedicated to bone as presented from both scientists and histotechnologists actively working in the field. This forum will also be a perfect opportunity to network with professionals within the "hard tissue niche". We hope to see you in August! Check out the NSH website @ www.nsh.org to download a copy of the brochure or feel free to contact Jack L Ratliff, NSH Hard Tissue Committee Chair @ 317-281-1975 or jratliff@biomimetics.com for more information. PROGRAM AT A GLANCE Registration Fees Member: $119 Non Member: $159 Schedule at Glance: 7:30am-8:00am Registration & Contintental Breakfast 8:00am-9:00am Bone Biology 101 Presented by Damien Laudier,BS,HTL(ASCP),QIHC, Laudier Histology, New York, NY 9:00am-10:30am In Vivo Models for Musculoskeletal Research Presented by Timothy Ganey,PhD, Atlanta Medical Center, Atlanta, GA 10:30am-10:45am Refreshment Break 10:45am-12:15pm Concepts for Specimen Management of Samples for Decalcified Paraffin Processing Presented by Robert A. Skinner,HTL(ASCP), University of Arkansas for Medical Sciences 12:15pm-1:15pm Lunch on Your Own 1:15pm-2:45pm Resin Histology: A Practical Approach for Demonstrating Undemineralized Bone Presented by Jack L. Ratliff, BA, BioMimetic Therapeutics, Inc., Franklin, TN 2:45pm-3:00pm Refreshment Break 3:00pm-4:00pm Skeletal Analysis with MicroCT: What You Need To Know And Why You Need To Know It Presented by Daniel S. Perrien, PhD, Vanderbilt Center for Bone Biology, Nashville, TN 4:00pm-5:00pm Bone Histomorphometry Presented by Damien Laudier,BS, HTL(ASCP),QIHC, Laudier Histology, New York, NY Travel Arrangements Lodging Doubletree Hotel Philadelphia 237 S. Broad Street, Philadelphia, PA 19107 Reservations via Phone: 215.893.1668 Registrants are responsible for hotel / travel arrangements. We have secured a small block of rooms at the discounted group rate of $125.00 plus applicable taxes. If you make reservations via phone please inform the agent that you are with the National Society for Histotechnology Group. Hotel Deadline is July 14, 2010. Getting to the Forum For directions, parking rates and airport shuttle information please visit the Doubletree's website. If you are flying the the Forum the Philadelphia International Airport is 9 miles from the hotel. Contact NSH Travel Services for best rate on air fare. Call 877.510.4747 between 8:30am-5:30pm, CT M-F or book on-line at www.nshtvl.com Cancellation/Substitution Requests All cancellations must be received in writing by July 14, 2010 to receive a full refund. Cancellations received after July 14, 2010 and no shows to the forum are non-refundable. Substitutions are accepted at any time. We must receive this request in writing. Individuals registering after July 14, 2010 will not be eligible for a refund. _________________________________________________________________ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2 ------------------------------ Message: 11 Date: Tue, 15 Jun 2010 07:55:28 -0400 From: Teri.Hallada@midmichigan.org Subject: [Histonet] Antibody Validation To: histonet@pathology.swmed.edu Message-ID: <8839B08E3ED7364E8CBBD53882C984D514102B37@MAILSRV01.midmichigan.net> Content-Type: text/plain; charset=us-ascii I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation. I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. ------------------------------ Message: 12 Date: Tue, 15 Jun 2010 05:56:55 -0700 (PDT) From: Rena Fail Subject: [Histonet] RE Antibody validation To: histonet@lists.utsouthwestern.edu Message-ID: <513753.56918.qm@web180308.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Teri New lots of Antibodies recieved?by a?lab should be validated?by ?that laboratory.? Thus proving the new lots produce similar results sa the old under the same?set of circumstances.?Processing, ?Retrevial methods, thickness of sections,?techniques etc?used in your laboratory contribute to the outcome. ?????? Having the Ab validated by the vendor does not verify that it works in your laboratory with your procedures. Rena Fail ----- Original Message ---- From: "Teri.Hallada@midmichigan.org" To: histonet@pathology.swmed.edu Sent: Tue, June 15, 2010 7:55:28 AM Subject: [Histonet] Antibody Validation I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation.? I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law.? The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited.? If you have received this email in error, please advise by immediate reply.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 15 Jun 2010 09:51:04 -0400 From: Brandi Higgins Subject: [Histonet] fume hood To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, Our hospital is doing some renovation and we need to look into new fume hoods for our new location. Currently we have one fume hood over our grossing area, and one fume hood in our coverslipping area (two different rooms). The hospital wants to put our grossing room and histo/cyto rooms together. I am still going to need two separate hoods. Does anyone have any experience/knowledge/input about fume hoods? I'm trying to look into the ductless ones, although I imagine changing the filters will end up being more expensive over time (I have no idea what would be involved in running a duct/vent). Also I have seen a benchtop downdraft type that sucks the air down, and does not have a top. It is advertised as being good for xylene. Does anyone use this in their coverslipping area? Any input would be greatly appreciated. I'm pretty clueless on the whole issue. I want to make sure that what I get will be safe for me and my coworker as we will be spending most of our day in this room. Any input is appreciated! Thank You! Brandi Higgins, BS, HT(ASCP) ------------------------------ Message: 14 Date: Tue, 15 Jun 2010 07:49:34 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Antibody Validation To: histonet@pathology.swmed.edu, Teri.Hallada@midmichigan.org Message-ID: <99966.29247.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Teri: You are right about the validations you propose although I am not surprised that your vendor does not think it is necessary. They are in the business of selling and you are in the business of assuring the high?quality of your work to obtaining the most accurate work for patients' sake. There is where the difference resides. Ignore your vendor and keep validating your protocols. Ren? J. --- On Tue, 6/15/10, Teri.Hallada@midmichigan.org wrote: From: Teri.Hallada@midmichigan.org Subject: [Histonet] Antibody Validation To: histonet@pathology.swmed.edu Date: Tuesday, June 15, 2010, 7:55 AM I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation.? I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law.? The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited.? If you have received this email in error, please advise by immediate reply.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Tue, 15 Jun 2010 07:53:03 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] fume hood To: histonet@lists.utsouthwestern.edu, Brandi Higgins Message-ID: <422333.48491.qm@web65705.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Over the counter fumes hood with filters are very effcicient and cheaper than running the vent ducts, especially in a large building. They also have the advantage that they can be moved to another place if necessary. Those without a cover are not that efficient. Ren? J. --- On Tue, 6/15/10, Brandi Higgins wrote: From: Brandi Higgins Subject: [Histonet] fume hood To: histonet@lists.utsouthwestern.edu Date: Tuesday, June 15, 2010, 9:51 AM Hello, Our hospital is doing some renovation and we need to look into new fume hoods for our new location.? Currently we have one fume hood over our grossing area, and one fume hood in our coverslipping area (two different rooms).? The hospital wants to put our grossing room and histo/cyto rooms together.? I am still going to need two separate hoods.? Does anyone have any experience/knowledge/input about fume hoods?? I'm trying to look into the ductless ones, although I imagine changing the filters will end up being more expensive over time (I have no idea what would be involved in running a duct/vent).? Also I have seen a benchtop downdraft type that sucks the air down, and does not have a top.? It is advertised as being good for xylene. Does anyone use this in their coverslipping area?? Any input would be greatly appreciated.? I'm pretty clueless on the whole issue.? I want to make sure that what I get will be safe for me and my coworker as we will be spending most of our day in this room.? Any input is appreciated!? Thank You! Brandi Higgins, BS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 15 Jun 2010 09:19:46 -0600 From: WILLIAM DESALVO Subject: RE: [Histonet] fume hood To: , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" There are two units we use in our labs and I suggest looking into the Mopec BF600 Downdraft Workcell. This works great for H&E/FS stain lines and other areas w/ open containers of chemicals. I also suggest considering the Labconco Protector XVS Ventilation Station for counter top hood with easy access, the unit is connected to outside air extraction. There are many other options and you should do a search on the internet for more options. William DeSalvo, B.S., HTL(ASCP) Production Manager, Sonora Quest laboratories Chair, NSH QCC > Date: Tue, 15 Jun 2010 09:51:04 -0400 > From: brandihiggins@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] fume hood > > Hello, > > Our hospital is doing some renovation and we need to look into new fume > hoods for our new location. Currently we have one fume hood over our > grossing area, and one fume hood in our coverslipping area (two different > rooms). The hospital wants to put our grossing room and histo/cyto rooms > together. I am still going to need two separate hoods. Does anyone have > any experience/knowledge/input about fume hoods? I'm trying to look into > the ductless ones, although I imagine changing the filters will end up being > more expensive over time (I have no idea what would be involved in running a > duct/vent). Also I have seen a benchtop downdraft type that sucks the air > down, and does not have a top. It is advertised as being good for xylene. > Does anyone use this in their coverslipping area? Any input would be > greatly appreciated. I'm pretty clueless on the whole issue. I want to > make sure that what I get will be safe for me and my coworker as we will be > spending most of our day in this room. Any input is appreciated! Thank > You! > > Brandi Higgins, BS, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3 ------------------------------ Message: 17 Date: Tue, 15 Jun 2010 15:28:42 +0000 From: Alexia Francisca N??ez Parra Subject: RE: [Histonet] Uneven fluorescent staining To: lita de foro Message-ID: Content-Type: text/plain; charset="iso-8859-1" Thank you so much for your answer Anatoli! - I tried the citrate buffer boiling system and I obtained the same unreliable results with NeuN. Some animals showed a great NeuN staining and others did not. I really thought at that point that my perfusion was the problem and I switched from making my own PFA4% to buying a 16%PFA from Electron Microscopy Sciences, which I think solved the problem. Now I'm obtaining a good NeuN staining but only in some regions of the tissue. - I am using the Rat monoclonal from Abcam (1:250) - I'll consider the new method using Edu labeling for new experiment and will try the ProLong Gold antifade, thank you very much for the advice. Alexia Nunez University of Maryland, College Park > Subject: RE: [Histonet] Uneven fluorescent staining > Date: Tue, 15 Jun 2010 10:26:32 -0400 > From: AGleiberman@cbiolabs.com > To: alexia_fran@hotmail.com > > Alexia, > 1. HCl treatment could be sometimes problematic - many epitopes are quite sensitive to it, so your NeuN antibody will work not in optimal conditions. You can replace acid treatment with citrate buffer boiling - the same treatment that is used for paraffin sections. It will denature DNA as effectively as HCl treatment. > 2. You did not mention what antibody you are using for BrdU - the best are rat monoclonal from Abcam (much better than any mouse monoclonal or sheep or goat polyclonal I have tested) - and you can easy combine them with mouse monoclonal against NeuN (I believe, only mouse anti-NeuN exist). > 3. For future experiments I recommend switch from BrdU labeling to EdU labeling (Click-iT? EdU Alexa Fluor? 488 HCS Assay from Invitrogen) - this technique is more sensitive for studying DNA synthesis than BrdU, staining is faster (30min), does not involve neither denaturation step nor antibody and can be easily combined with any other marker staining. To do antigen staining after EdU visualization, that is simple chemical reaction, you need wash thoroughly your slides and perform your antibody staining. Additional benefit is - much better structure of nuclei because denaturation step sometimes affects them. In addition, because of high sensitivity you can decrease concentration of nucleotide substitute (EdU) 5-10 times in comparison with BrdU that can be crucial for long-term experiments because all these nucleotide analogues (especially BrdU) can affect cell fate in long run. > 4. Finally, I recommend to replace Vectashield with ProLong Gold antifade (Invitrogen) with or without DAPI. It is better for fluorochrome preservation and it will harden overnight - you don't need any nail polish > > Anatoli Gleiberman, PhD > Director of Histopathology > Cleveland Biolabs, Inc > 73 High Street > Buffalo, NY 14203 > phone:716-849-6810 ext.354 > fax:716-849-6817 > e-mail: AGleiberman@cbiolabs.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alexia Francisca N??ez Parra > Sent: Monday, June 14, 2010 6:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Uneven fluorescent staining > > > Hello everyone!! > The Histonet Archives have been always very helpful to me, so I decided to become a member and ask a personal question to the experts. > > I am doing NeuN and BrdU double staining in the olfactory bulb, following the protocol described below. I have always had some trouble with the NeuN staining, obtaining sometimes good or no staining at all. I tried to trouble shoot with the intracardial perfusion and even bought a new NeuN antibody (Chemicon). I am having now, however, another problem. I observed an uneven NeuN and BrdU staining. There are some parts of the section that get a really good and bright staining and others that do not get any staining at all (even though they should). > > Can anyone give a hint on what to review or change? > > Thank you so much!!! > > Alexia > > 1. Intracardial perfusion with cold PBS and cold 4%PFA. > 2. The brains are postfixed for 4 hrs in PFA 4% at 4C and immersed in sucrose 30% ON. After that, the brains are embedded in tissue tek and store at -80. > 3. Sections of 20um are obtained using a cryostat and collected using positive charged slides. > > > > > > > > > > > > > > > 4. > Allow the slides to reach the RT > > > 5. > Warm slides at 55C x 10' > > 6. > Outline slides with Immedge pen > > 7. > Hydrate: PBS 2 x 5', final quick wash with ddH20 > > 8. > Soak slides in 2N HCl for 1 hr at 37C > > 9. > Neutralize washing the slides with 0.1M Na tretaborate buffer > pH8.5, 3 x 10' > > 10. > Wash with PBS 2 x 10' > > 11. > Wash with PBS-T 1 x 10' > > > > 12. > Block at RT with 10% of Donkey serum in PBST x 2hrs (60uL on each section) > > 13. > First antibody: incubate at 4C with first antibody diluited in > PBST with 2.5% Donkey serum ON (16-24hrs) > > 14. > Wash with PBST 4x10' at RT > > 15. > Secondary antibody: dilute 1:750 alexa antibodies in PBST with > 2.5% Donkey serum x 2 hrs at RT > > 16. > Wash with PBST 1 x 10', and then with PBS 3x 10' > > > > 17. > Mount with Vectashield and seal using transparent nail polish. > > > > > > _________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _________________________________________________________________ ------------------------------ Message: 18 Date: Tue, 15 Jun 2010 11:49:28 -0400 From: BSullivan@shorememorial.org Subject: Re: [Histonet] Antibody Validation To: Rene J Buesa Cc: Teri.Hallada@midmichigan.org, histonet-bounces@lists.utsouthwestern.edu, histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I agree with Rene. All lot to lot and new antibodies need to be checked for consistency. This is part of your validation process. This even holds true for pre-dilutes which are already tested by the manufacturer for optimum results. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Rene J Buesa To Sent by: histonet@pathology.swmed.edu, histonet-bounces@ Teri.Hallada@midmichigan.org lists.utsouthwest cc ern.edu Subject Re: [Histonet] Antibody Validation 06/15/2010 10:49 AM Teri: You are right about the validations you propose although I am not surprised that your vendor does not think it is necessary. They are in the business of selling and you are in the business of assuring the high?quality of your work to obtaining the most accurate work for patients' sake. There is where the difference resides. Ignore your vendor and keep validating your protocols. Ren? J. --- On Tue, 6/15/10, Teri.Hallada@midmichigan.org wrote: From: Teri.Hallada@midmichigan.org Subject: [Histonet] Antibody Validation To: histonet@pathology.swmed.edu Date: Tuesday, June 15, 2010, 7:55 AM I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation.? I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law.? The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited.? If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Tue, 15 Jun 2010 08:57:21 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] Antibody Validation To: "Rene J Buesa" , , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C63F5@EXCHANGECLUSTER.yumaregional.local> Content-Type: text/plain; charset="us-ascii" There are other issue that you have to take into consideration with both instruments, With digital image analysis you also have to look at the questions that CAP just instituted for this. This includes the machine being run by someone that can do high complexity testing. This means that you have to have a tech do this machine, not a TA or Lab aid. There are also issue with consistent calibration of the Image analysis machines, continued education of the techs, and validating not only anitbodies but continued validation of the image algorythms.. Do not listen to vendors, rely on your instinct and validate. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ ------------------------------ Message: 20 Date: Tue, 15 Jun 2010 10:01:16 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] Antibody Validation - long response To: "Rene J Buesa" , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Bottom line it's not the vendors responsibility to validate their equipment or antibodies in your lab. Some vendors may help you do this, but ultimately the lab needs to validate the equipment and IHC in their lab. The vendors normally calibrate the equipment prior to shipment and once they set the instrument up in your lab, they should be able to provide you with the documentation that states that they calibrated the instrument. Your instruments need to be calibrated prior to being validated. As far as your scanner goes some vendors can provide validation, but it's at a cost and that cost is not cheap depending upon what you actually want validated. If you are using the scanner and associated algorithms for analysis then you need to validate that separately. There are several steps required to validate a scanner - 1. you validate the scanner 2. if you are using a database to store your images then that also may need to be validated and 3. if you are using algorithms that provide you with data then those algorithms need to be validated. For example prior to running a validation protocol on a tissue processor its needs to be calibrated for temperature. All of your major equipment needs to be on a calibration schedule. We calibrate all of our instruments once a year and validation is completed only once unless we change the instrument location or how we use the instrument. Pipettors are calibrated every 6 months. All instruments are validated it may just be a one pager for the basic lab equipment but instruments like the tissue processor, slide staining, IHC stainer and scanner require written protocols some of these are 80 pages in length and go into great detail. The same goes for your antibodies. Antibodies are validated initially with 25 tissue samples (10 strongly positive tissues, 10 moderate to weakly positive tissues and 5 tissues that have no reactivity) This type of validation is required for routine antibodies, prognostic markers such as Her-2, ER and PR require additional tissue samples. New lots require 3 tissue samples one strongly positive on moderate to weakly positive and one negative. If you change the antibody source or detection system or retrieval it needs to be validated again - This information comes from the paper Standarization of Immunohistochemistry from CAP its available on line - I have a copy if you need it. There are also new guidelines for ER/PR and a new article on validation of ER/PR in the June issue of Archives of pathology from CAP. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, June 15, 2010 8:50 AM To: histonet@pathology.swmed.edu; Teri.Hallada@midmichigan.org Subject: Re: [Histonet] Antibody Validation Teri: You are right about the validations you propose although I am not surprised that your vendor does not think it is necessary. They are in the business of selling and you are in the business of assuring the high?quality of your work to obtaining the most accurate work for patients' sake. There is where the difference resides. Ignore your vendor and keep validating your protocols. Ren? J. --- On Tue, 6/15/10, Teri.Hallada@midmichigan.org wrote: From: Teri.Hallada@midmichigan.org Subject: [Histonet] Antibody Validation To: histonet@pathology.swmed.edu Date: Tuesday, June 15, 2010, 7:55 AM I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation.? I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law.? The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited.? If you have received this email in error, please advise by immediate reply.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 15 **************************************** From JEllin <@t> yumaregional.org Tue Jun 15 12:14:28 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Jun 15 12:14:26 2010 Subject: [Histonet] Antibody Validation - long response In-Reply-To: Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C63F7@EXCHANGECLUSTER.yumaregional.local> Well said Elizabeth Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, June 15, 2010 9:01 AM To: Rene J Buesa; histonet@pathology.swmed.edu; Teri.Hallada@midmichigan.org Subject: RE: [Histonet] Antibody Validation - long response Bottom line it's not the vendors responsibility to validate their equipment or antibodies in your lab. Some vendors may help you do this, but ultimately the lab needs to validate the equipment and IHC in their lab. The vendors normally calibrate the equipment prior to shipment and once they set the instrument up in your lab, they should be able to provide you with the documentation that states that they calibrated the instrument. Your instruments need to be calibrated prior to being validated. As far as your scanner goes some vendors can provide validation, but it's at a cost and that cost is not cheap depending upon what you actually want validated. If you are using the scanner and associated algorithms for analysis then you need to validate that separately. There are several steps required to validate a scanner - 1. you validate the scanner 2. if you are using a database to store your images then that also may need to be validated and 3. if you are using algorithms that provide you with data then those algorithms need to be validated. For example prior to running a validation protocol on a tissue processor its needs to be calibrated for temperature. All of your major equipment needs to be on a calibration schedule. We calibrate all of our instruments once a year and validation is completed only once unless we change the instrument location or how we use the instrument. Pipettors are calibrated every 6 months. All instruments are validated it may just be a one pager for the basic lab equipment but instruments like the tissue processor, slide staining, IHC stainer and scanner require written protocols some of these are 80 pages in length and go into great detail. The same goes for your antibodies. Antibodies are validated initially with 25 tissue samples (10 strongly positive tissues, 10 moderate to weakly positive tissues and 5 tissues that have no reactivity) This type of validation is required for routine antibodies, prognostic markers such as Her-2, ER and PR require additional tissue samples. New lots require 3 tissue samples one strongly positive on moderate to weakly positive and one negative. If you change the antibody source or detection system or retrieval it needs to be validated again - This information comes from the paper Standarization of Immunohistochemistry from CAP its available on line - I have a copy if you need it. There are also new guidelines for ER/PR and a new article on validation of ER/PR in the June issue of Archives of pathology from CAP. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, June 15, 2010 8:50 AM To: histonet@pathology.swmed.edu; Teri.Hallada@midmichigan.org Subject: Re: [Histonet] Antibody Validation Teri: You are right about the validations you propose although I am not surprised that your vendor does not think it is necessary. They are in the business of selling and you are in the business of assuring the high?quality of your work to obtaining the most accurate work for patients' sake. There is where the difference resides. Ignore your vendor and keep validating your protocols. Ren? J. --- On Tue, 6/15/10, Teri.Hallada@midmichigan.org wrote: From: Teri.Hallada@midmichigan.org Subject: [Histonet] Antibody Validation To: histonet@pathology.swmed.edu Date: Tuesday, June 15, 2010, 7:55 AM I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation.? I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law.? The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited.? If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From cls71877 <@t> sbcglobal.net Tue Jun 15 13:32:23 2010 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Tue Jun 15 13:32:26 2010 Subject: [Histonet] alcohol monitoring Message-ID: <947322.95349.qm@web81207.mail.mud.yahoo.com> Hello, I was recently approached by my management about monitoring the alcohol in the lab?? I monitor for xylene and formalin, but I have never heard of doing so for alcohol.? Does anyone else do this at their lab?? Is there potentially a different type of testing to check for this?? Any guidance is much appreciated. Thanks, Cristi From camayton <@t> gmail.com Tue Jun 15 13:38:12 2010 From: camayton <@t> gmail.com (Cathy Mayton) Date: Tue Jun 15 13:38:26 2010 Subject: [Histonet] liquidating lab equipment Message-ID: I have a few pieces of lab equipment left over from when I closed and liquidated the lab. I have the following: 1) Shandon paraffin embeddding center with heated forceps 2) VIP 2000 tissue processor 3) 6 foot fume hood (1/2 of base cabinet is flammable storage) 4) 2 Well Diamond wire saws (model 4240) 5) Exakt grinder all of the equipment was being used right up to the last week we closed the lab -- Cathy Mayton From histology <@t> medsurgpath.com Tue Jun 15 14:24:52 2010 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Tue Jun 15 14:24:55 2010 Subject: [Histonet] Part-Time Job Opening in Portland, OR Message-ID: <6046e41f9c597c5cc03063caede27ac7.squirrel@webmail.integra.net> Part-Time Laboratory Technician Part-time laboratory technician needed for private histology laboratory. Job duties include accessioning and handling medical specimens. Applicant should be familiar with human anatomy, have attention to detail, and be self motivated. HTL/HT (or equivalent) encouraged to apply. Bachelor?s degree in biology, chemistry or other basic science with a minimum GPA 3.2 required. Salary DOE Email katelin@medsurgpath.com or fax resumes to (503) 670-9754 Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. From rjbuesa <@t> yahoo.com Tue Jun 15 14:41:19 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 15 14:41:24 2010 Subject: [Histonet] alcohol monitoring In-Reply-To: <947322.95349.qm@web81207.mail.mud.yahoo.com> Message-ID: <905495.35359.qm@web65705.mail.ac4.yahoo.com> Never done it, never requested, really unnecessary. Perhaps the "requester" was trying to "push his/her pencil" harder than others in creating a new layer of bureaucratic work to just?be "more busy"! Ren? J. --- On Tue, 6/15/10, Cristi stephenson wrote: From: Cristi stephenson Subject: [Histonet] alcohol monitoring To: "Histo Net" Date: Tuesday, June 15, 2010, 2:32 PM Hello, I was recently approached by my management about monitoring the alcohol in the lab?? I monitor for xylene and formalin, but I have never heard of doing so for alcohol.? Does anyone else do this at their lab?? Is there potentially a different type of testing to check for this?? Any guidance is much appreciated. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Tue Jun 15 15:32:43 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Jun 15 15:32:49 2010 Subject: [Histonet] alcohol monitoring In-Reply-To: <947322.95349.qm@web81207.mail.mud.yahoo.com> References: <947322.95349.qm@web81207.mail.mud.yahoo.com> Message-ID: What type of "alcohol" are you being asked to test and why? OSHA does establish limits for Isopropyl Alcohol (PEL 400 ppm) because it is an irritant. If you are doing tissue processing w/out xylene, then you are probably using isoporpyl alcohol and should test personnel maintaining the processor. I think you will need additional information before you can set a plan. William DeSalvo, B.S., HTL(ASCP) > Date: Tue, 15 Jun 2010 11:32:23 -0700 > From: cls71877@sbcglobal.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] alcohol monitoring > > Hello, > I was recently approached by my management about monitoring the alcohol in the lab? I monitor for xylene and formalin, but I have never heard of doing so for alcohol. Does anyone else do this at their lab? Is there potentially a different type of testing to check for this? Any guidance is much appreciated. > Thanks, > Cristi > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From Bauer.Karen <@t> mayo.edu Tue Jun 15 15:42:22 2010 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Jun 15 15:42:27 2010 Subject: [Histonet] alcohol monitoring In-Reply-To: <947322.95349.qm@web81207.mail.mud.yahoo.com> References: <947322.95349.qm@web81207.mail.mud.yahoo.com> Message-ID: <53FC421CC200C5429929EDE6C3676F30F0A352@msgebe34> Hi Cristi, We have been monitoring alcohol fumes for many years. Our hospital safety officer orders badges and we wear them on our lab coats for 15 minutes and another one for 8 hours. Only one tech needs to wear them in the lab, not everybody. We usually do it on a day where we will handle more alcohol... Like cleaning the processors and recycling. We also wear the badges for xylene and formalin fumes. Again, one for 15 minutes and another for 8 hours. Once we are done, we put them back in their designated bags and call the safety officer to pick them up. He sends them off to wherever he orders them and they send back the report. Very easy... If you want, I could put you in touch with our safety officer so you can get further information. Good luck, Karen Karen L. Bauer HT/HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Tuesday, June 15, 2010 1:32 PM To: Histo Net Subject: [Histonet] alcohol monitoring Hello, I was recently approached by my management about monitoring the alcohol in the lab?? I monitor for xylene and formalin, but I have never heard of doing so for alcohol.? Does anyone else do this at their lab?? Is there potentially a different type of testing to check for this?? Any guidance is much appreciated. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From elciba <@t> hotmail.com Tue Jun 15 16:37:24 2010 From: elciba <@t> hotmail.com (ricky hachy) Date: Tue Jun 15 16:37:28 2010 Subject: [Histonet] liquidating lab equipment In-Reply-To: References: Message-ID: Hello Cathy, I can be interessed on these 2 eqs. Shandon paraffin embeddding center with heated forceps VIP 2000 tissue processor Do you have any pictures ? Regards Ricky > Date: Tue, 15 Jun 2010 13:38:12 -0500 > From: camayton@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] liquidating lab equipment > > I have a few pieces of lab equipment left over from when I closed and > liquidated the lab. I have the following: > > 1) Shandon paraffin embeddding center with heated forceps > 2) VIP 2000 tissue processor > 3) 6 foot fume hood (1/2 of base cabinet is flammable storage) > 4) 2 Well Diamond wire saws (model 4240) > 5) Exakt grinder > > all of the equipment was being used right up to the last week we closed the > lab > > -- > Cathy Mayton > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2 From saby_joseph_a <@t> yahoo.com Tue Jun 15 17:17:49 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Tue Jun 15 17:17:55 2010 Subject: [Histonet] Fume hood In-Reply-To: <4C1776D5020000F40005B815@GWIA2.umm.edu> References: <4C1776D5020000F40005B815@GWIA2.umm.edu> Message-ID: <56849.74630.qm@web114419.mail.gq1.yahoo.com> Brandi- Having gone through and even designed lab renovations, my advice is: Have a different hood for each function if possible.? Renovations cannot see into the future to know what technologies you might need later.? An extra hood now can very easily seem like too few later.? Also, many functions need to be separated, a fact?that administrators will never understand.? You might need vents over your processors, a vented area for special stains, a hood for grossing and a hood for stainers/ coverslippers.? Cytology may need several hoods also.??If genetic determination requirements become standard, will this be in your lab? Emergency power for each hood.? Also processors and other necessary equipment (stainers, etc.). Desktop height for many histology applications, especially grossing, embedding and sectioning.? Otherwise, the ergonomics are terrible. I would suggest backdraft enclosed hoods for grossing, vented covered areas for processors, stainers/coverslippers.? If using xylenes, enclose as much as possible and use backdraft.? Be sure to keep in mind that with backdraft hoods, there may need to be face opening restrictions to achieve the majic number of 100 cfm you need for safety. If you have anyother questions, please get back with me. Joe Saby, BA HT ________________________________ From: Walter Benton To: histonet@lists.utsouthwestern.edu Sent: Tue, June 15, 2010 12:49:25 PM Subject: [Histonet] Fume hood Brandi, I have been through several renovations and highly recommend that you get a hood that exhaust outside. Depending upon what applications you plan to carry out under the hood, filters may not do the trick. It is also important to have someone from your facilities or engineering department conduct an air exchange analysis to make sure that you have proper airflow and exchanges within the room, since the two operations are being combined. I would also recommend that you have your area monitored for air quality while performing the various tasks that you perform now in you current configuration to see if you are OSHA compliant or whatever regulatory body you wish to follow. Downdraft ventilation is great for Xylene, because Xylene vapor is heavier than air, thus the reason those systems work well. Feel free to reach out if you have any other questions. Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 >>> On 6/15/2010 at 12:31 PM, wrote: Message: 13 Date: Tue, 15 Jun 2010 09:51:04 -0400 From: Brandi Higgins Subject: [Histonet] fume hood To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, Our hospital is doing some renovation and we need to look into new fume hoods for our new location.? Currently we have one fume hood over our grossing area, and one fume hood in our coverslipping area (two different rooms).? The hospital wants to put our grossing room and histo/cyto rooms together.? I am still going to need two separate hoods.? Does anyone have any experience/knowledge/input about fume hoods?? I'm trying to look into the ductless ones, although I imagine changing the filters will end up being more expensive over time (I have no idea what would be involved in running a duct/vent).? Also I have seen a benchtop downdraft type that sucks the air down, and does not have a top.? It is advertised as being good for xylene. Does anyone use this in their coverslipping area?? Any input would be greatly appreciated.? I'm pretty clueless on the whole issue.? I want to make sure that what I get will be safe for me and my coworker as we will be spending most of our day in this room.? Any input is appreciated!? Thank You! Brandi Higgins, BS, HT(ASCP) This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcampbell <@t> vdxpathology.com Tue Jun 15 18:16:11 2010 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Tue Jun 15 18:16:22 2010 Subject: [Histonet] NY licensing Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FDE@VDXSERVER01.vdxpathology.local> I'm looking into requirements for getting licensed as a histotech in NY and I am a little unsure of whether my educational experience will be considered a substantial equivalent to their requirements. They require education from a Histology program or something equivalent. I graduated from a 4 year university (UC Davis) with a B.S. in Animal Science (includes all science courses--biology, chem, organic chemistry, physics, anatomy etc) but I never specifically took a class on histology and its techniques used in the lab. Do you still think they will consider me eligible or would they require me to complete a histology program? I have yet to find the chance to call them since they close at 4:45 ET and I am on the west coast. Thanks in advance, Jennifer Campbell From CIngles <@t> uwhealth.org Tue Jun 15 19:30:23 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Jun 15 19:31:45 2010 Subject: [Histonet] Antibody Validation References: <99966.29247.qm@web65716.mail.ac4.yahoo.com> Message-ID: Funny, I'd think the vendors would want you to do tons of your own validation, as it uses up antibody faster, ergo they get to sell you more antibody! :) In a feisty mood today. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Tue 6/15/2010 9:49 AM To: histonet@pathology.swmed.edu; Teri.Hallada@midmichigan.org Subject: Re: [Histonet] Antibody Validation Teri: You are right about the validations you propose although I am not surprised that your vendor does not think it is necessary. They are in the business of selling and you are in the business of assuring the high quality of your work to obtaining the most accurate work for patients' sake. There is where the difference resides. Ignore your vendor and keep validating your protocols. Ren? J. --- On Tue, 6/15/10, Teri.Hallada@midmichigan.org wrote: From: Teri.Hallada@midmichigan.org Subject: [Histonet] Antibody Validation To: histonet@pathology.swmed.edu Date: Tuesday, June 15, 2010, 7:55 AM I am being questioned by our vendor as to why we need to validate our automated immunostainer and image analysis instrument. They would like documentation pertaining to the requirement of validation and the number of specimens utilized for validation. I am requesting that each antibody be validated on the instrument against a previously validated instrument. Additionally, I am requesting that each new lot of antibody be validated upon receipt against previously ran specimens. This would also apply to the image analysis antibodies. (Her2 has been validated by FISH.) The vendor has apparently polled users in the area and this is not a standard protocol, therefore the request for documentation. I think it is pretty clearly stated by CAP in the Quality Management In Anatomic Pathology. Any other suggestions? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jbirkner <@t> colabserv.com Wed Jun 16 07:46:21 2010 From: jbirkner <@t> colabserv.com (Jeff Birkner) Date: Wed Jun 16 07:46:26 2010 Subject: [Histonet] alcohol monitoring In-Reply-To: <905495.35359.qm@web65705.mail.ac4.yahoo.com> Message-ID: 29CFR 1910.1000 Table Z-1 states that the Limit for air contamination with Ethyl Alcohol is 1000 ppm, xylene is 100 ppm, formaldehyde is 0.75 ppm for an 8 hour TWA, isopropyl alcohol is 400 ppm, methyl alcohol is 200 ppm. Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, June 15, 2010 2:41 PM To: Histo Net; Cristi stephenson Subject: Re: [Histonet] alcohol monitoring Never done it, never requested, really unnecessary. Perhaps the "requester" was trying to "push his/her pencil" harder than others in creating a new layer of bureaucratic work to just?be "more busy"! Ren? J. --- On Tue, 6/15/10, Cristi stephenson wrote: From: Cristi stephenson Subject: [Histonet] alcohol monitoring To: "Histo Net" Date: Tuesday, June 15, 2010, 2:32 PM Hello, I was recently approached by my management about monitoring the alcohol in the lab?? I monitor for xylene and formalin, but I have never heard of doing so for alcohol.? Does anyone else do this at their lab?? Is there potentially a different type of testing to check for this?? Any guidance is much appreciated. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. From dmikita <@t> wmcnet.org Wed Jun 16 08:04:03 2010 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Wed Jun 16 08:11:25 2010 Subject: [Histonet] excessive IHC background staining Message-ID: <4C18790F.1875.003A.0@wmcnet.org> Hello Everyone, I have been running into an issue with Synaptophysin with antigen retrieval. I currently use a piece of pancreas for the Synaptophysin control on the same slide as the patient tissue. When I run this IHC on a non-necrotic piece of tissue, it works great. When the patient tissue is necrotic I get excessive background on both the patient and control tissue. What is going on here. We use a peroxidase block, but no avidin-biotin block or protein block. I have tried an avidin-biotin block at the urging of technical support, but it did not change anything. Also, a negative control (patient tissue with negative control reagent) is clean. Thanks, Daryl Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 Phone: 307-577-2198 Fax: 307-577-2731 From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Jun 16 08:11:00 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Jun 16 08:14:18 2010 Subject: [Histonet] RE: NY licensing In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF8181FDE@VDXSERVER01.vdxpathology.local> References: <5658CBDB9EAE6545ABE50D2563D81BF8181FDE@VDXSERVER01.vdxpathology.local> Message-ID: They used to have grandfathering for technicians that we trained and worked in other labs. I believe that they have discontinued this. Your best bet is to call them directly and get some guidance. The website is http://www.op.nysed.gov/prof/clt/clpbroch.htm I believe that you'll still have to take the exam, but I could be wrong. It is a very long and confusing process. Good luck. They really aren't sure what they are doing either. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell [jcampbell@vdxpathology.com] Sent: Tuesday, June 15, 2010 7:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NY licensing I'm looking into requirements for getting licensed as a histotech in NY and I am a little unsure of whether my educational experience will be considered a substantial equivalent to their requirements. They require education from a Histology program or something equivalent. I graduated from a 4 year university (UC Davis) with a B.S. in Animal Science (includes all science courses--biology, chem, organic chemistry, physics, anatomy etc) but I never specifically took a class on histology and its techniques used in the lab. Do you still think they will consider me eligible or would they require me to complete a histology program? I have yet to find the chance to call them since they close at 4:45 ET and I am on the west coast. Thanks in advance, Jennifer Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Jun 16 10:31:15 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jun 16 10:32:00 2010 Subject: [Histonet] RELIA Histology Careers Bulletin 6-16-10 How are you staying cool this Summer? Message-ID: Hi Histonetters! What are you doing to stay cool this Summer? What is your best tip to beat the summer heat? Living in Central Florida I can use all the help with cooling off that I can get!! I recently discovered a really refreshing summer drink and thought I would pass it along. It is called an Arnold Palmer ? it is a 50/50 blend of iced tea and lemonade. Whatever your favorite summer pastime is, this is sure to be a great way to cool off. I also wanted to give you a list of my current open positions. These are exciting opportunities with some of the finest employers in the country. All of my client offer excellent compensation, benefits and relocation assistance. All of these jobs are full time and permanent. HISTOLOGY MANAGEMENT Pathology Supervisor ? Fresno, CA Histology Supervisor ? Long Island, NY NYS license NOT required Histology Lab Manager ? Phoenix, AZ HISTOLOGY TECHS Hagerstown, MD Phoenix, AZ Austin, TX North Shore of Boston Boston, MA Cape Cod, MA Suffern, NY IMMUNOHISTOCHEMISTRY SPECIALISTS Long Island, NY Dallas, TX Boston, MA If you or anyone you know is interested in hearing more about any of these opportunities or would like to discuss a customized job search please contact me. I can be reached toll free at 866-607-3542 or e-mail me at relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net < http://home.earthlink.net/~relia1> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From jbirkner <@t> colabserv.com Wed Jun 16 11:53:23 2010 From: jbirkner <@t> colabserv.com (Jeff Birkner) Date: Wed Jun 16 11:53:29 2010 Subject: [Histonet] Non-Glyoxal based formalin substitutes Message-ID: I am wondering what types of Non-glyoxal based formalin substitutes folks are using. We currently use Prefer (glyoxal based fixative) as our formalin substitute. We are very satisfied with this product for most cases; however, we are having problems with inflammatory skin cases. We need to be able to accurately evaluate the absence or presence and relative density of eosinophils and the absence or presence of extravasated red blood cells to differentiate between otherwise similar inflammatory skin diseases. Prefer (glyoxal) binds to the argine site which inhibits eosin staining of eosinophils and it also lyses erythrocytes. Are there any formalin free substitutes that will work for this situation? Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. From jerrysedgewick <@t> gmail.com Wed Jun 16 12:04:54 2010 From: jerrysedgewick <@t> gmail.com (Jerry (Gerald) Sedgewick) Date: Wed Jun 16 12:05:40 2010 Subject: [Histonet] 1st Annual Imaging in Research Course In-Reply-To: References: Message-ID: <4C190436.9040000@gmail.com> I wanted to let people know about this new course some colleagues and I will be giving about microscopic photography including the correct techniques when acquiring, post-processing, and adjusting images for outputs along with techniques that work for segmenting complex, biological images (for subsequent image analysis). The course is called "1st Annual Imaging in Research Course: Ethics, Acquisition, Post-Processing, Output and Segmenting" and it being held at the University of Minnesota Continuing Education Center in Minneapolis/St. Paul, Minnesota, August 16 - 19, 2010. It is sponsored by the Histochemical Society and the Adobe Corporation. Please go to [1]http://www.imagingandanalysis.com/seminars.html for more information. Jerry Sedgewick -- Jerry (Gerald) Sedgewick Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output." Sedgewick Initiatives 965 Cromwell Avenue Saint Paul, MN 55114 651-788-2261 [2]jerrysedgewick@gmail.com [3]http://www.quickphotoshop.com [4]http://www.rawlight.com [5]http://www.jerrysedgewick.com References 1. http://www.imagingandanalysis.com/seminars.html 2. mailto:jerrysedgewick@gmail.com 3. http://www.quickphotoshop.com/ 4. http://www.rawlight.com/ 5. http://www.jerrysedgewick.com/ From Farnana <@t> nehealth.com Wed Jun 16 12:52:46 2010 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Wed Jun 16 12:52:57 2010 Subject: [Histonet] Fwd: Hi Jennifer, References: <4C18D6C3.26ED.00D9.1@nehealth.com> Message-ID: <4C18D72E.26ED.00D9.1@nehealth.com> >>> Amy Farnan 6/16/2010 1:51 PM >>> Hi Jennifer, If you go to http://www.op.nysed.gov/prof/clt/ it will give you the histology courses that you will need to work in NY state. You will need more than just a biology degree, but take a look. If you have any further questions please don't hesitate to ask. I have worked closely with the NYSED on the licensing for histology. Regards, Amy Farnan NYSHS BOD Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From TJJ <@t> stowers.org Wed Jun 16 15:23:48 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Wed Jun 16 15:24:07 2010 Subject: [Histonet] RE: Certification In-Reply-To: <6b4dcafd-607c-43a1-a33c-d88d73ec6150@exchmb-01.stowers-institute.org> References: <6b4dcafd-607c-43a1-a33c-d88d73ec6150@exchmb-01.stowers-institute.org> Message-ID: Dear Joyce, I too am in Kansas City, MO and Missouri does not have any state license or requirement for needing the HT certification in order to cut permanent slides. That may be a requirement from your employer, they can set the minimum standard for what you can and cannot do based on your education, training and certifications. For instance, because I do not have my HTL, I may be ineligible for certain job openings because they have made that a condition of employment. I cannot speak to the other potential distance learning courses out there. I would do a google search for "Histology Distance Learning" or some such permutation of it and see what comes up. Additionally you may get some other responses from the Histonet. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From janderson <@t> halozyme.com Wed Jun 16 15:30:51 2010 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Wed Jun 16 15:31:43 2010 Subject: [Histonet] intervertebral disc Message-ID: <5F7CC9B788911848A79BC83453A3D6530412587710@Tomlinson.hti.com> Greetings. What is the best way to process intervertebral disc tissue from pig? Right now I have 4 intact vertebrae with attached discs in formalin. Should I just use a sharp knife? Thanks so much for your expertise. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. From Nacaela.Johnson <@t> USONCOLOGY.COM Wed Jun 16 15:53:22 2010 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Wed Jun 16 15:53:25 2010 Subject: [Histonet] (no subject) Message-ID: <6DBD71C31D7E444482E5D3DFBC202D2602074B7A@txhous1eb012.uson.usoncology.int> Does anyone know a good overnight processing protocol for bone marrows fixed in Acid Zinc Formalin? My tissue is washing off the slides. I have tried adhesive slides and they do not help. I am currently using a processing protocol that many use for bone marrows. I think the AZF may be dehydrating the tissue and this process protocol is making it worse. Any suggestions for a protocol? I am not able to change fixatives. Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From TJJ <@t> stowers.org Wed Jun 16 16:24:36 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Wed Jun 16 16:24:45 2010 Subject: [Histonet] Re: Non-Glyoxal based formalin substitutes Message-ID: Jeffrey, I can't speak directly to the question of other formalin free substitutes that might work for your situation with the inflammatory skin cases. All I would know to tell you is try an alcohol fixative. It's going to change the look of all the cells, and how they stain, and that might cause other issues. It would almost definitely impact any immunohistochemistry you might have to do. What I did think of is this, even though the glyoxal lyses erythrocytes, you still see their ghosts. So you know they're there. Is that not good enough? As for the eosinophils, have you tried or thought about another type of stain to demonstrate those? Would the Luna method work (Weigert's iron hematoxylin/biebrich scarlet)? What about mordanting or post-fixing the slide prior to doing an H&E? Maybe in Bouins or 10% NBF? Do you think it's beyond detection at this point? Just some random thoughts, probably worth about what you paid for them. Good luck! Teri Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From renafail <@t> bellsouth.net Wed Jun 16 19:12:29 2010 From: renafail <@t> bellsouth.net (Rena Fail) Date: Wed Jun 16 19:12:37 2010 Subject: [Histonet] (no subject) In-Reply-To: <6DBD71C31D7E444482E5D3DFBC202D2602074B7A@txhous1eb012.uson.usoncology.int> References: <6DBD71C31D7E444482E5D3DFBC202D2602074B7A@txhous1eb012.uson.usoncology.int> Message-ID: <537354.5349.qm@web180316.mail.gq1.yahoo.com> Are you washing the bone marrows before processing? Years ago ;) when I used acidic zenkers we washed the BM before processing then post fixed in formalin on the processor. The overnight processing was formalin until the machine started 1 hour in 70% ethanol,?an hour in 3 changes 95% ethanol, 3, changes 100% ethanol, 3 changes paraffin. for rush processing it was 20 minutes in each. THe bone marrows were cut at 3 microns.?i didn't have a problem with tissue washing off.? Rena Fail ----- Original Message ---- From: "Johnson, Nacaela" To: histonet@lists.utsouthwestern.edu Sent: Wed, June 16, 2010 4:53:22 PM Subject: [Histonet] (no subject) Does anyone know a good overnight processing protocol for bone marrows fixed in Acid Zinc Formalin? My tissue is washing off the slides. I have tried adhesive slides and they do not help. I am currently using a processing protocol that many use for bone marrows. I think the AZF may be dehydrating the tissue and this process protocol is making it worse. Any suggestions for a protocol? I am not able to change fixatives. Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office:? 913-234-0576 Fax:? 913-433-7639 Email:? Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dcojita <@t> tampabay.rr.com Wed Jun 16 20:03:09 2010 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Wed Jun 16 20:03:13 2010 Subject: [Histonet] supervsior position in Ormond Beach Message-ID: Hello fellow netters: A friend of mine still has an opening for a supervisory position in Ormond Beach, Florida. This lab is a new, State-of-the-Art facility with a primary focus in dermatology. It will be a fantastic opportunity for the right person! I understand they may have a part-time position as well. If anyone is interested, I'll be happy to pass the contact info on to you. Thanks, Diane From lpwenk <@t> sbcglobal.net Wed Jun 16 20:18:15 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Jun 16 20:18:40 2010 Subject: [Histonet] alcohol monitoring In-Reply-To: Message-ID: You might want to do the alcohol monitoring once, just to prove your lab is SOOOO far below the 1000 ppm. Then never do it again, because you would probably have to do 100x the amount of tissue alcohol use, to come close to the OSHA limits. Peggy Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeff Birkner Sent: Wednesday, June 16, 2010 8:46 AM To: Rene J Buesa; Histo Net; Cristi stephenson Subject: RE: [Histonet] alcohol monitoring 29CFR 1910.1000 Table Z-1 states that the Limit for air contamination with Ethyl Alcohol is 1000 ppm, xylene is 100 ppm, formaldehyde is 0.75 ppm for an 8 hour TWA, isopropyl alcohol is 400 ppm, methyl alcohol is 200 ppm. Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, June 15, 2010 2:41 PM To: Histo Net; Cristi stephenson Subject: Re: [Histonet] alcohol monitoring Never done it, never requested, really unnecessary. Perhaps the "requester" was trying to "push his/her pencil" harder than others in creating a new layer of bureaucratic work to just?be "more busy"! Ren? J. --- On Tue, 6/15/10, Cristi stephenson wrote: From: Cristi stephenson Subject: [Histonet] alcohol monitoring To: "Histo Net" Date: Tuesday, June 15, 2010, 2:32 PM Hello, I was recently approached by my management about monitoring the alcohol in the lab?? I monitor for xylene and formalin, but I have never heard of doing so for alcohol.? Does anyone else do this at their lab?? Is there potentially a different type of testing to check for this?? Any guidance is much appreciated. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eyee <@t> dpmginc.com Thu Jun 17 00:10:07 2010 From: eyee <@t> dpmginc.com (Ellen Yee) Date: Thu Jun 17 00:10:55 2010 Subject: [Histonet] New CAP question ANP.22760 Message-ID: <20100617051007.697d4065@mail.dpmginc.com> How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee From sfeher <@t> CMC-NH.ORG Thu Jun 17 08:13:45 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Jun 17 08:13:49 2010 Subject: [Histonet] fume hood In-Reply-To: References: Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B7544D@exchange.cmc-nh.org> Hi Brandy, I have recently had the opportunity to build a Path lab from scratch. In the design we decided to completely separate the grossing area from the microtomy and IHC area of the lab. We built a "room within a room", made it negative pressure, installed 2 Thermo elevating grossing stations that are vented to the outside. Since we are using the hospital ventilation system in addition to the blowers built in to the back draft, downdraft capabilities of the grossing stations, we were able to set these to pull at 500 cfm each. We also put 2 Peloris processors, with their own charcoal filters, within this room. The result is that we are well under the limits for all fumes and, in the event we get fresh tissue, we can segregate the area from the rest of the lab. Many labs that have to do autopsy on babies or near full term fetus' use their grossing stations to do so. Since we are in a separate area, we can block these procedures from view. We also put in a Labconco Fume hood (vented to the outside) in the IHC area of the lab and a Thermo Bio Hood in the cytoprep area. This has all worked out very well for us and it affords us the opportunities to have these items in place for future growth. A renovation done correctly, with an eye towards strategic planning for the future, will go a long way towards saving the hospital money in the long run. Steve Feher Pathology Supervisor Catholic Medical Center Manchester, NH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Tuesday, June 15, 2010 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fume hood Hello, Our hospital is doing some renovation and we need to look into new fume hoods for our new location. Currently we have one fume hood over our grossing area, and one fume hood in our coverslipping area (two different rooms). The hospital wants to put our grossing room and histo/cyto rooms together. I am still going to need two separate hoods. Does anyone have any experience/knowledge/input about fume hoods? I'm trying to look into the ductless ones, although I imagine changing the filters will end up being more expensive over time (I have no idea what would be involved in running a duct/vent). Also I have seen a benchtop downdraft type that sucks the air down, and does not have a top. It is advertised as being good for xylene. Does anyone use this in their coverslipping area? Any input would be greatly appreciated. I'm pretty clueless on the whole issue. I want to make sure that what I get will be safe for me and my coworker as we will be spending most of our day in this room. Any input is appreciated! Thank You! Brandi Higgins, BS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Jun 17 08:32:56 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jun 17 08:33:00 2010 Subject: [Histonet] Thermo Slide Mate & Print Mate Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3E44@is-e2k3.grhs.net> Is anyone using the Thermo Slide Mate & Print Mate with CoPath as your AP LIS? I am trying to decide if I am going to look at this as a stand alone process or integrate it with my AP LIS. I would like to hear from those that use it both ways and if it is okay to contact you off line. Thanks, Mike From laurie.colbert <@t> huntingtonhospital.com Thu Jun 17 09:12:04 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Jun 17 09:12:10 2010 Subject: [Histonet] Thermo Slide Mate & Print Mate Message-ID: <57BE698966D5C54EAE8612E8941D768308F25175@EXCHANGE3.huntingtonhospital.com> Mike, We have the Print Mate and 5 Slide Mates. We are not connected to our LIS - we use it as a stand alone process. Everything is set up very basic and the system works very well for us. Feel free to contact me offline. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, June 17, 2010 6:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Slide Mate & Print Mate Is anyone using the Thermo Slide Mate & Print Mate with CoPath as your AP LIS? I am trying to decide if I am going to look at this as a stand alone process or integrate it with my AP LIS. I would like to hear from those that use it both ways and if it is okay to contact you off line. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Reuel.Cornelia <@t> tsrh.org Thu Jun 17 12:10:10 2010 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu Jun 17 12:10:35 2010 Subject: [Histonet] how to prevent foldings on femoral head cartilage tissue Message-ID: <4C1A10A2020000C50007B27B@mail.TSRH.ORG> Hello histonetters especailly hard tissue group I have a pig femoral head bone tissue embedded in paraffin and I have a hard time getting rid of the folding problem. I tried to remedy by lowering my temperature to 38 C and putting them in 5% alcohol before placing them in water bath I still have a lots of folding formation on some areas of the cartilage. Is there any other technique to remedy this problem. I appreciate your help. Thank you. reuel From sbaldwin <@t> mhhcc.org Thu Jun 17 12:24:22 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Thu Jun 17 12:25:07 2010 Subject: [Histonet] Breast Specimen handling Message-ID: Hi histonetters Was wondering if anyone saw the new gui specimen handling ? I am sure you have.&nbs is wondering is if you have a large specimen and do tissue for processing what would the agent be to apply specimen to stop penetration of the formlalin before the 72 hour ti me arrives? Would it be sodium chloride? Can someone help? < Thanks Pathology Supervisor Kathy Baldwin, S Memorial Hospital and Health Care&nbs [1]sbaldwin@m Ph 812-482-0210, 482-0216, Fax 81 Pager 812-481-0897 Confidential information, Authorized use only. References 1. 3D"mailto:sbaldwin@mhhcc.org" From dmikita <@t> wmcnet.org Thu Jun 17 12:36:08 2010 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Thu Jun 17 12:43:30 2010 Subject: [Histonet] RE:New CAP question ANP.22760 Message-ID: <4C1A0A59.1875.003A.0@wmcnet.org> Hello, We are using our appropriate antibody control tissues run side by side to do the antibody and detection system lot tests. One using the old lot number of reagents and one with the new lot number of reagents. TTYL, Daryl Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 Phone: 307-577-2198 Fax: 307-577-2731 From cls71877 <@t> sbcglobal.net Thu Jun 17 12:49:06 2010 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Thu Jun 17 12:49:11 2010 Subject: [Histonet] alcohol monitoring In-Reply-To: References: Message-ID: <593656.50942.qm@web81207.mail.mud.yahoo.com> Thank you all so much for the guidance!? I appreciate all of the input. Sincerely, Cristi ________________________________ From: Lee & Peggy Wenk To: Jeff Birkner ; Rene J Buesa ; Histo Net ; Cristi stephenson Sent: Wed, June 16, 2010 6:18:15 PM Subject: RE: [Histonet] alcohol monitoring You might want to do the alcohol monitoring once, just to prove your lab is SOOOO far below the 1000 ppm. Then never do it again, because you would probably have to do 100x the amount of tissue alcohol use, to come close to the OSHA limits. Peggy Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeff Birkner Sent: Wednesday, June 16, 2010 8:46 AM To: Rene J Buesa; Histo Net; Cristi stephenson Subject: RE: [Histonet] alcohol monitoring 29CFR 1910.1000 Table Z-1 states that the Limit for air contamination with Ethyl Alcohol is 1000 ppm, xylene is 100 ppm, formaldehyde is 0.75 ppm for an 8 hour TWA, isopropyl alcohol is 400 ppm, methyl alcohol is 200 ppm. Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA? 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, June 15, 2010 2:41 PM To: Histo Net; Cristi stephenson Subject: Re: [Histonet] alcohol monitoring Never done it, never requested, really unnecessary. Perhaps the "requester" was trying to "push his/her pencil" harder than others in creating a new layer of bureaucratic work to just?be "more busy"! Ren? J. --- On Tue, 6/15/10, Cristi stephenson wrote: From: Cristi stephenson Subject: [Histonet] alcohol monitoring To: "Histo Net" Date: Tuesday, June 15, 2010, 2:32 PM Hello, I was recently approached by my management about monitoring the alcohol in the lab?? I monitor for xylene and formalin, but I have never heard of doing so for alcohol.? Does anyone else do this at their lab?? Is there potentially a different type of testing to check for this?? Any guidance is much appreciated. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited.? If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system.? If you have any questions concerning this message, please contact the sender. The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited.? If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system.? If you have any questions concerning this message, please contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Jun 17 12:53:45 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jun 17 12:53:54 2010 Subject: [Histonet] how to prevent foldings on femoral head cartilage tissue In-Reply-To: <4C1A10A2020000C50007B27B@mail.TSRH.ORG> Message-ID: Sometimes placing them on a hot plate at about 60C will help get out the folds, the paraffin needs to melt and the sections need to turn clear then take it off the hot plate. If you leave it too long on the hotplate the articular cartilage may fold over on itself. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia Sent: Thursday, June 17, 2010 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to prevent foldings on femoral head cartilage tissue Hello histonetters especailly hard tissue group I have a pig femoral head bone tissue embedded in paraffin and I have a hard time getting rid of the folding problem. I tried to remedy by lowering my temperature to 38 C and putting them in 5% alcohol before placing them in water bath I still have a lots of folding formation on some areas of the cartilage. Is there any other technique to remedy this problem. I appreciate your help. Thank you. reuel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> shorememorial.org Thu Jun 17 12:55:47 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Thu Jun 17 12:58:07 2010 Subject: [Histonet] Breast Specimen handling In-Reply-To: Message-ID: Sara, Funny that this comes up now. I attended a meeting last week and this was one of the topics. It was suggested that the tissue be stored in 70% alcohol. I personally have not done this but I am going to start doing this. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Sara Baldwin/mhhcc.org " To Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Breast Specimen handling 06/17/2010 01:24 PM Hi histonetters Was wondering if anyone saw the new gui= delines for breast Cancer specimen handling ? I am sure you have.&nbs= p; What my Pathologist is wondering is if you have a large specimen and do = not use all the tissue for processing what would the agent be to apply= to the specimen to stop penetration of the formlalin before the 72 hour ti me arrives? Would it be sodium chloride? Can someone help? <= /DIV> Thanks Pathology Supervisor Kathy Baldwin, S= CT (ASCP) Memorial Hospital and Health Care&nbs= p;Center [1]sbaldwin@m= hhcc.org Ph 812-482-0210, 482-0216, Fax 81= 2-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. References 1. 3D"mailto:sbaldwin@mhhcc.org" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Thu Jun 17 13:16:49 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Jun 17 13:17:14 2010 Subject: [Histonet] how to prevent foldings on femoral head cartilage tissue In-Reply-To: <4C1A10A2020000C50007B27B@mail.TSRH.ORG> References: <4C1A10A2020000C50007B27B@mail.TSRH.ORG> Message-ID: Reuel, Do the opposite and turn up the temp on the waterbath and let the section float out a little before the wax starts to break up. You can even gently use the forcepts to tease out any folds and this will definitely help to release any wrinkles in the cartilage. Then, make sure the section is free of large droplets of water before transfer of the slide to the slide warmer. Next, let slide sit on slide warmer until the wax is melted and then bake the section for a few minutes at a higher temperature to firmly secure the section to the glass slide. Bob Skinner at UAMS showed me this technique at the beginning of the year and it works nicely, especially for even larger human femoral heads! Jack > Date: Thu, 17 Jun 2010 12:10:10 -0500 > From: Reuel.Cornelia@tsrh.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] how to prevent foldings on femoral head cartilage tissue > > Hello histonetters especailly hard tissue group > I have a pig femoral head bone tissue embedded in paraffin and I have a hard time getting rid of the folding problem. I tried to remedy by lowering my temperature to 38 C and putting them in 5% alcohol before placing them in water bath I still have a lots of folding formation on some areas of the cartilage. Is there any other technique to remedy this problem. I appreciate your help. Thank you. > > reuel > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1 From Maria.Katleba <@t> stjoe.org Thu Jun 17 13:21:03 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Jun 17 13:22:18 2010 Subject: [Histonet] Breast Specimen handling In-Reply-To: References: Message-ID: Problem, if you store it in alcohol, you compromise the potential for IHC, and other tests. It somehow messes with the antibodies. But I am no expert.... Supposedly its why people switched to formalin fixation instead of alcoholic fixatives.... Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Thursday, June 17, 2010 10:56 AM To: Sara Baldwin/mhhcc.org Cc: Histo Net; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Breast Specimen handling Sara, Funny that this comes up now. I attended a meeting last week and this was one of the topics. It was suggested that the tissue be stored in 70% alcohol. I personally have not done this but I am going to start doing this. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Sara Baldwin/mhhcc.org " To Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Breast Specimen handling 06/17/2010 01:24 PM Hi histonetters Was wondering if anyone saw the new gui= delines for breast Cancer specimen handling ? I am sure you have.&nbs= p; What my Pathologist is wondering is if you have a large specimen and do = not use all the tissue for processing what would the agent be to apply= to the specimen to stop penetration of the formlalin before the 72 hour ti me arrives? Would it be sodium chloride? Can someone help? <= /DIV> Thanks Pathology Supervisor Kathy Baldwin, S= CT (ASCP) Memorial Hospital and Health Care&nbs= p;Center [1]sbaldwin@m= hhcc.org Ph 812-482-0210, 482-0216, Fax 81= 2-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. References 1. 3D"mailto:sbaldwin@mhhcc.org" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From ratliffjack <@t> hotmail.com Thu Jun 17 13:25:08 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Jun 17 13:25:18 2010 Subject: [Histonet] intervertebral disc In-Reply-To: <5F7CC9B788911848A79BC83453A3D6530412587710@Tomlinson.hti.com> References: <5F7CC9B788911848A79BC83453A3D6530412587710@Tomlinson.hti.com> Message-ID: Jennifer, What do you wish to accomplish histologically? Do you only wish to see the disc material? Do you care about the cranial and caudal vertebral bodies? Are you wanting to perform IHC? Please tell me a little more so that I can provide you with a more detailed options. I am assuming that this is an IVD project and maybe you only wish to see the treatment of the nucleus pulposus? Jack > From: janderson@halozyme.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 16 Jun 2010 13:30:51 -0700 > Subject: [Histonet] intervertebral disc > > Greetings. > What is the best way to process intervertebral disc tissue from pig? Right now I have 4 intact vertebrae with attached discs in formalin. Should I just use a sharp knife? > Thanks so much for your expertise. > > Jennifer M. Anderson, Scientist > Halozyme Therapeutics, Inc. > 11388 Sorrento Valley Road > San Diego, CA 92121 > 858-704-8333 (office) > janderson@halozyme.com > > > > ________________________________ > The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1 From Mary_Joy <@t> smhwecare.com Thu Jun 17 13:32:24 2010 From: Mary_Joy <@t> smhwecare.com (Mary_Joy@smhwecare.com) Date: Thu Jun 17 13:32:41 2010 Subject: [Histonet] release of tissues Message-ID: Hi all, How would you handle a request from a surgeon, f gallstones or any tissue, to a patient? Assuming a 'Rele Tissue' request form, indicating the biohazard staus of the tissue, is signed by the patient, is this commom practice? Thanks for your responses, Mary Sue CONFID contain from disclosure regulations. This the designated ad addressee, you are hereby distribution of this e-ma unlawful and may subject you received this e-mail and its attachmen the St. Mary's Hospital Helpdesk at (301) 4 e-mail and its attachments from your computer. Than attention. Please consider the environment: email? CONFIDENTIALITY NOTICE: This contain confidential information which from disclosure by Federal and State confidenti regulations. This e-mail and attachments, if any, are the designated addressee only. If you are not the designated addressee, you are hereby notified that any disclosure, copying, or distri unlawful and may received this e-mail and it the St. Mary's Hospital Helpdesk at e-mail and its attachments from your computer attention. Please consider the environment: do you really need to print this email? From Carol.Fields <@t> Northside.com Thu Jun 17 13:36:58 2010 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Thu Jun 17 13:37:14 2010 Subject: [Histonet] Immuno Message-ID: <731941C266951A47BEF11E5EFAAED9C903920DA5@nsmvexch01.northside.local> Hi All, Does anyone out there know a lab that is able to do a Human Herpes Virus, Type 6 immuno? Thanks for any help on this. We haven't been able to find a reference lab that does this. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From KSly <@t> cmch.org Thu Jun 17 14:45:16 2010 From: KSly <@t> cmch.org (Karen Sly) Date: Thu Jun 17 14:45:36 2010 Subject: [Histonet] release of tissues In-Reply-To: References: Message-ID: <4C1A432F.BAF2.00B1.0@cmch.org> Mary Sue, Our hospital regular releases gallstones to patients and/or their doctors. We make a record of it in our computer system as to where and to who the gallstones were sent/released to. Karen Sly BS Biology Pathology Department Central Michigan Community Hospital Mt Pleasant MI. Please consider the environment before printing this e-mail Please consider the environment before printing this e-mail From eyee <@t> dpmginc.com Thu Jun 17 19:20:37 2010 From: eyee <@t> dpmginc.com (Ellen Yee) Date: Thu Jun 17 19:21:32 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: 57BE698966D5C54EAE8612E8941D768308F2518D@EXCHANGE3.huntingtonhospital.com Message-ID: <20100618002037.0ab34f26@mail.dpmginc.com> Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _____ From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] To: Ellen Yee [mailto:eyee@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Fri Jun 18 06:37:28 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Jun 18 06:37:33 2010 Subject: [Histonet] cryojane question Message-ID: <888472.46698.qm@web50307.mail.re2.yahoo.com> Happy Friday! We recently purchased a cryojane?for sectioning frozen?human?and rat cartilage.? I am having some issues with my cartilage sections.? I am using the 4X slides with 2 flashes.? The sections look beautiful, until I put?them into buffer to do staining.? I am getting folds and the sections are lifting off during IHC staining.? I am?staining either by hand (on flat trays, old-fashioned style) or in the sequenza racks.? Luckily, the cartilage is not?not coming off the slides completely, so I have been able to get the?data I need.? I was wondering if anyone had any tips for me to prevent the folds or the lift-off. Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From a.thotakura <@t> imperial.ac.uk Fri Jun 18 07:18:14 2010 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Fri Jun 18 07:18:43 2010 Subject: [Histonet] Message-ID: Dear All, I have a PFA fixed liver frozen sections and I want to stain for cd45.1, cd45.2 and f4/80. Can you guys please help me with the protocols for the staining. I would like to do Immunofluroscence. Thank you all in advance. Many Thanks, Anil Kumar. From trathborne <@t> somerset-healthcare.com Fri Jun 18 07:36:42 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Jun 18 07:36:47 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: <20100618002037.0ab34f26@mail.dpmginc.com> Message-ID: Should these slides be retained? If so, how long? Or is it enough just to have the documentation? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Ellen Yee Sent: Thursday, June 17, 2010 8:21 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _____ From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] To: Ellen Yee [mailto:eyee@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From kmerriam2003 <@t> yahoo.com Fri Jun 18 08:09:24 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Jun 18 08:09:27 2010 Subject: [Histonet] ISH on histogel cell pellets Message-ID: <76799.58987.qm@web50306.mail.re2.yahoo.com> Hi Everyone, We have some cells that we have processed and embedded in histogel (thanks?to everyone for the protocols?that were sent to me a couple of weeks?ago). We are planning to do IHC and ISH on these pellets.??Will the histogel interfere with the ISH?procedure at all? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From talulahgosh <@t> gmail.com Fri Jun 18 09:10:12 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jun 18 09:10:18 2010 Subject: [Histonet] cryojane question In-Reply-To: <888472.46698.qm@web50307.mail.re2.yahoo.com> References: <888472.46698.qm@web50307.mail.re2.yahoo.com> Message-ID: Histonetters, I just looked up what a cryojane was, and it's pretty neat! Does anyone else use this? The one flaw seems to be that you can only put one section on a slide (or at least that the way it's depicted here: http://www.instrumedics.com/cryojanetapetransferprocess.htm ) which makes it pretty time consuming. Also does the uv step interfere with in situ protocols? I guess not since the DNA/RNA is already transcribed and fixed and therefore wouldn't be mutated. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose From alyssa <@t> alliedsearchpartners.com Fri Jun 18 09:10:30 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Jun 18 09:10:35 2010 Subject: [Histonet] Dermatopathology Message-ID: Hello, I know this might be a little out of the rhelm of histonet but, I thought I would give it a try. I am struggling to figure out how a Dermatopathologist is paid? Is he/she paid per slide? Or is he/she paid a base salary, or both? I would just really like an idea. Thank you for any help. -- Alyssa Peterson, Director of Candidate Recruitment Allied Search Partners T: 888.388.7571 ext. 101 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From rcharles <@t> state.pa.us Fri Jun 18 09:38:28 2010 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Jun 18 09:38:39 2010 Subject: [Histonet] IHC kits Message-ID: <3809C163DC1DA54AA534B3C7794D07B65A8FBDC0E5@ENHBGMBX01.PA.LCL> Happy Friday to all, Dako has once again raised their prices to the point that I'm asked to find another IHC labeling kit that is cheaper. Could those doing IHC please recommend a kit that is as good as Dako's LSAB2? Thanks so much. Roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 From liz <@t> premierlab.com Fri Jun 18 09:43:30 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jun 18 09:43:37 2010 Subject: [Histonet] cryojane question In-Reply-To: Message-ID: Emily You can put more than one section on a slide if you need to, but in our experience it does not work as nicely as depicted all of the time, it can be a bit tricky to work with on undecalcifed bone and harder tissues. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, June 18, 2010 8:10 AM To: Kim Merriam; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryojane question Histonetters, I just looked up what a cryojane was, and it's pretty neat! Does anyone else use this? The one flaw seems to be that you can only put one section on a slide (or at least that the way it's depicted here: http://www.instrumedics.com/cryojanetapetransferprocess.htm ) which makes it pretty time consuming. Also does the uv step interfere with in situ protocols? I guess not since the DNA/RNA is already transcribed and fixed and therefore wouldn't be mutated. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Jun 18 09:46:28 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jun 18 09:46:49 2010 Subject: [Histonet] ISH on histogel cell pellets In-Reply-To: <76799.58987.qm@web50306.mail.re2.yahoo.com> Message-ID: Kim the histogel should not interfere with the IHC staining, I'm not sure about the ISH Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, June 18, 2010 7:09 AM To: Histonet Subject: [Histonet] ISH on histogel cell pellets Hi Everyone, We have some cells that we have processed and embedded in histogel (thanks?to everyone for the protocols?that were sent to me a couple of weeks?ago). We are planning to do IHC and ISH on these pellets.??Will the histogel interfere with the ISH?procedure at all? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Fri Jun 18 09:51:06 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Jun 18 09:51:16 2010 Subject: [Histonet] PARTS FOR HACKER/MEISEI COVERSLIPPER Message-ID: <24A4826E8EF0964D86BC5317306F58A542598BCC11@mmc-mail.ad.mhsil.com> We have a glass coverslipper that is not currently working because we need a diaphragm for the vacuum pump in it. Our repair people tell us we have to get it from Japan and it will take eight weeks. Anybody have an idea where we can get a new or used vacuum pump for this coverslipper? We have budgeted for a new coverslipper in October but that is a long way off. Surely someone has an old coverslipper that we could get the pump off of for a decent price. Any help would be greatly appreciated. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From sgoebel <@t> xbiotech.com Fri Jun 18 09:55:09 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Jun 18 09:55:31 2010 Subject: [Histonet] cryojane question Message-ID: <20100618075508.9e2d9aa830e8449a2412eb1e4f2f067e.40f938ec9e.wbe@email04.secureserver.net> Does this kind of seem unnecessary? What are the applicatio would make this Cryo-tape beneficial. I have always used the paintbrush method which takes 3 seconds, and has always worked perfect.&nbs looks cool and a need this for; it's defini Sarah Goebel, B.A., HT (ASCP)< Histotechnician XBiotech USA Inc. < 8201 East Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: Re: [Histonet] cryojane question From: Emily Sours <[1]talulahgos Date: Fri, June 18, 2010 7:10 am To: Kim Merriam <[2]kmerriam200 [3]histonet@lists.utsou Histonetters, I just looked up what a cryojane was, and it's pretty neat! Does anyone else use this? The one flaw seems to be that you can only put one section on a slide (or a least that the way it's depicted here: [4]htt which makes it pretty time consuming. Also does the uv step interfere with in situ protocols? I guess not since the DNA/RNA is already transcribed and fixed and therefore wouldn't be mutated. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose _______________________________________________ Histonet mailing list [5]Histonet@lists.utsou [6]http: References 1. 3D"mailto://talulahgosh@gmail.com"/ 2. 3D"mailto://kmerriam2003@yahoo.com"/ 3. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 4. 3D"http://www.instrumedics.com/cryojanetapetransferprocess.htm" 5. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 6. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From sgoebel <@t> xbiotech.com Fri Jun 18 10:00:14 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Jun 18 10:00:27 2010 Subject: [Histonet] Dermatopathology Message-ID: <20100618080014.9e2d9aa830e8449a2412eb1e4f2f067e.78c864ee32.wbe@email04.secureserver.net> Most pathologists I have worked with (some are specialized as der mapathologists) work with a group if it's a clinical setting. There a guys, derm g with own part o crap ton!). The tw pay them a salary, but I charge. Good luck, I think it' out with the docs! =) Sarah Goebel, Histotechnician XBiotech US 8201 East Riverside Dr. Bldg 4 Suite 100 A (512)386-5107 -------- Original Message -------- Subject: [Histonet] Dermatopathology From: Alyssa Peterson <[1]alyssa@alliedsearchpartners.com> Date: Fri, June 18, 2010 7:10 am To: [2]histonet@lists.u Hello, I know this might be a little out of the rhelm of histonet but, I thought I would give it a try. I am struggling to figure out how a Dermatopathologist is paid? Is he/she paid per slide? Or is he/she paid a base salary, or both? I would just really like an idea. Thank you for any help. -- Alyssa Peterson, Director of Candidate Recruitment Allied Search Partners T: 888.388.7571 ext. 101 F: 888.388.7572 [3]www.alliedsearchpartners.co This email including its attachments is intended only for the confidential< you are not the intended< distribution or copying of this message< prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. _______________________________________________ Histonet mailing list [4]Histonet@lists.utsou [5]http: References 1. 3D"mailto://alyssa@alliedsearchpartners.c=/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"http://www.alliedsearchpartners.com"/ 4. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From sgoebel <@t> xbiotech.com Fri Jun 18 10:11:01 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Jun 18 10:12:02 2010 Subject: [Histonet] ISH on histogel cell pellets Message-ID: <20100618081101.9e2d9aa830e8449a2412eb1e4f2f067e.b5269c0a6c.wbe@email04.secureserver.net> Try using 0.9% plain 'ol agar. I use it all the time, it's then the histogel and it works fine with the IHCs. I don't kn about ISH, but it's a thought (and a super cheap trial?) < Sarah Goebel, B Histotechnician < XBiotech USA I 8201 East Riverside Dr. Bldg 4 Suite 100 Aust (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] ISH on histogel cell pellets From: "Liz Chlipala" <[1]liz@premie Date: Fri, June 18, 2010 7:46 am To: "Kim Merriam" <[2]kmerriam2 <[3]histonet@lists.u Kim the histogel should not interfere with the IHC staining, I'm not sure a Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 [4]www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: [5]histon [[6]mailto:histonet-bounces@lists.utsouthwestern.edu Kim Merriam Sent: Friday, June 18, 2010 7:09 AM To: Histonet Subject: [Histonet] ISH on histogel cell pellets Hi Everyone, We have some cells that we have processed and embedded in histogel (thanks& couple of we We are planning to do IHC and ISH on these pellets. Will the his togel interfere with the ISH procedure at all? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list [7]Histonet@lists.utsou [8]http: _______________________________________________ Histonet mailing list [9]Histonet@lists.utsou [10]http: References 1. 3D"mailto://liz@premierlab.com"/ 2. 3D"mailto://kmerriam2003@yahoo.com"/ 3. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 4. 3D"http://www.premierlab.com"/ 5. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 6. 3D"mailto:histonet-bounces 7. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 8. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 9. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From kalschev <@t> svm.vetmed.wisc.edu Fri Jun 18 10:16:47 2010 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Fri Jun 18 10:16:58 2010 Subject: [Histonet] porcine intervertebral disc Message-ID: <55CB4517A4A84E99A97857AC1D040755@vetmed.wisc.edu> Jennifer, If you can gently remove the disc, section appropriately and process for cross-section cuts you will get nice results. You might want to extend the paraffin times for very good infiltration. If the disc/bone placement needs to be in place, it becomes more difficult as the decal is hard on the disc tissue and porcine bone take a long time to decal. With disc/bone embedding and sectioning the soft and hard differences in the tissue are problematic. I suggest sectioning thicker. It can be done and the results beautiful! Vicki From talulahgosh <@t> gmail.com Fri Jun 18 10:23:08 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jun 18 10:23:19 2010 Subject: [Histonet] IHC kits In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B65A8FBDC0E5@ENHBGMBX01.PA.LCL> References: <3809C163DC1DA54AA534B3C7794D07B65A8FBDC0E5@ENHBGMBX01.PA.LCL> Message-ID: We use Vectastain kits and they always work well. I don't know if you can use them for your particular IHC but here's their site www.vectorlabs.com Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Fri, Jun 18, 2010 at 10:38 AM, Charles, Roger wrote: > Happy Friday to all, > Dako has once again raised their prices to the point that I'm asked to find > another IHC labeling kit that is cheaper. Could those doing IHC please > recommend a kit that is as good as Dako's LSAB2? > Thanks so much. > Roger > > Roger Charles > Microbiologist II > PA Veterinary Laboratory > 717-787-8808 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TJJ <@t> stowers.org Fri Jun 18 10:45:49 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri Jun 18 10:45:58 2010 Subject: [Histonet] Re: cryojane question Message-ID: To Kim, What thickness are you using to cut these? I'm simply amazed you're having trouble keeping them on using the 4x slides and 2 flashes. Have you tried using the 1/2x or even the 1x slides? Did they wash worse using these? Are they fixed at any time, or fresh frozen and sectioned? To Emily, I don't think the UV flash would affect the ISH at all. To Liz, We share your experience with it. The undecalcified bone and other hard tissues are very tricky to work with, and the quality of our efforts really do vary from day to day. Using this to section cartilage and decalcified bone should be pretty easy and satisfying. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From wdorsett-martin <@t> umc.edu Fri Jun 18 11:01:27 2010 From: wdorsett-martin <@t> umc.edu (Wanda A. Dorsett-Martin) Date: Fri Jun 18 11:02:48 2010 Subject: [Histonet] JB-4 Message-ID: <6E4AA1CF7F63E8499B665A73CF4F08150EFC240DB3@EXMBX2.ntummc.umsmed.edu> I am still struggling when I have a spare minute to get our "inherited" JB-4 Sorvall manual microtome to work for paraffin sectioning. Now the micron knob moves freely and I can't get it to stay set on the thickness. If I get it on say 5 microns as you are cutting it drifts so you never know what thickness you are going to get. Should I just scrap this old machine or does anyone know how I need to adjust something ? As you probably can tell I am not mechanically inclined. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From LSebree <@t> uwhealth.org Fri Jun 18 11:37:06 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Jun 18 11:37:13 2010 Subject: [Histonet] cryojane question In-Reply-To: References: <888472.46698.qm@web50307.mail.re2.yahoo.com> Message-ID: <8C023B4AB999614BA4791BAEB26E2738399ECF@UWHC-MAIL01.uwhis.hosp.wisc.edu> I've used it for serial frozen sections in the past; worked very well for that application. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, June 18, 2010 9:10 AM To: Kim Merriam; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryojane question Histonetters, I just looked up what a cryojane was, and it's pretty neat! Does anyone else use this? The one flaw seems to be that you can only put one section on a slide (or at least that the way it's depicted here: http://www.instrumedics.com/cryojanetapetransferprocess.htm ) which makes it pretty time consuming. Also does the uv step interfere with in situ protocols? I guess not since the DNA/RNA is already transcribed and fixed and therefore wouldn't be mutated. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Fri Jun 18 11:41:39 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Jun 18 11:41:44 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: <20100618002037.0ab34f26@mail.dpmginc.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> I don't think I can do this with the automated system we are currently using. Ventana. Does any other Ventana users know if you can do this in "parallel" Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Thursday, June 17, 2010 7:21 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _____ From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] To: Ellen Yee [mailto:eyee@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robinsoc <@t> mercyhealth.com Fri Jun 18 12:22:40 2010 From: Robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Fri Jun 18 12:22:57 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> References: <20100618002037.0ab34f26@mail.dpmginc.com> <661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> Message-ID: <4C1B6510.59BC.00AF.0@mercyhealth.com> I do this but it has to be separate runs or wait until the kit is just running out. I also keep the slides from the initial QC run and compare it with the new lot to assure same intensity staining is obtained when changing lots. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> "Mike Pence" 6/18/2010 11:41 AM >>> I don't think I can do this with the automated system we are currently using. Ventana. Does any other Ventana users know if you can do this in "parallel" Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Thursday, June 17, 2010 7:21 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _____ From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] To: Ellen Yee [mailto:eyee@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Jun 18 12:27:24 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Jun 18 12:27:29 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> References: <20100618002037.0ab34f26@mail.dpmginc.com> <661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> Message-ID: You'll have to use prep kit stickers and duplicate protocols to do it, but it is possible. You just need to copy the protocol and save it as another number, then change the primary antibody to a prep kit sticker save again and then put that sticker on the dispenser. Then you need to print stickers for both protocols and stick them on two controls slides and run. I admit its a little bit of a pain. Mark On Fri, Jun 18, 2010 at 9:41 AM, Mike Pence wrote: > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this in > "parallel" > > Mike > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents tested > in parallel with old lots? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using an > appropriate panel of control tissues.) > > Ellen Yee > _____ > > From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > To: Ellen Yee [mailto:eyee@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Jun 18 12:47:35 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Jun 18 12:50:56 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> References: <20100618002037.0ab34f26@mail.dpmginc.com>, <661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> Message-ID: I think that CAP means that you need to save the slide that you ran from the previous lot and compare it to the slide that you have stained with the new lot number. To see if they are sufficient diagnostic quality. Not put both lot numbers on the machine at the same time and then compare the slides? We run Dako machines and it would be tricky to put both numbers on the same machine. Although this is my interpretation. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpence@grhs.net] Sent: Friday, June 18, 2010 12:41 PM To: Ellen Yee; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 I don't think I can do this with the automated system we are currently using. Ventana. Does any other Ventana users know if you can do this in "parallel" Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Thursday, June 17, 2010 7:21 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _____ From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] To: Ellen Yee [mailto:eyee@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sweaver <@t> bbpllab.com Fri Jun 18 12:59:09 2010 From: sweaver <@t> bbpllab.com (Steve Weaver) Date: Fri Jun 18 12:59:18 2010 Subject: [Histonet] RE: Histonet Digest, Vol 79, Issue 21 In-Reply-To: <2322245925419378083681784825498> Message-ID: <6DB7235FE14F1E49ABC5794DF98C751F093DEE1A44@exsrv07> Any pros/cons with leica cm1950 against thermofisher hm550/fumigation option with cryostats? Two to four days a week using cryostat. Durability issues???? Thanks and enjoy the weekend. From MAUGER <@t> email.chop.edu Fri Jun 18 12:59:35 2010 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Fri Jun 18 13:01:21 2010 Subject: [Histonet] RE: IHC kits In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B65A8FBDC0E5@ENHBGMBX01.PA.LCL> References: <3809C163DC1DA54AA534B3C7794D07B65A8FBDC0E5@ENHBGMBX01.PA.LCL> Message-ID: <443F5B475A9BF647AB962E834884EBAD278D1277E3@EX7CCRPW03V1.chop.edu> The Leica Bond detection kits are better than LSAB. They are barcoded for the Bondmax machine-not sure if they offer packaged another way. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger [rcharles@state.pa.us] Sent: Friday, June 18, 2010 10:38 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC kits Happy Friday to all, Dako has once again raised their prices to the point that I'm asked to find another IHC labeling kit that is cheaper. Could those doing IHC please recommend a kit that is as good as Dako's LSAB2? Thanks so much. Roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Fri Jun 18 13:06:20 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Jun 18 13:06:47 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: References: <20100618002037.0ab34f26@mail.dpmginc.com> <661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> Message-ID: <24A4826E8EF0964D86BC5317306F58A542598BCCCE@mmc-mail.ad.mhsil.com> I'm kind of getting in on the middle of this but is anybody else doing this with prep kit stickers? I have not seen the new question but has anyone talked to CAP to get a clarification of what they mean on this question? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 12:27 PM To: Mike Pence Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 You'll have to use prep kit stickers and duplicate protocols to do it, but it is possible. You just need to copy the protocol and save it as another number, then change the primary antibody to a prep kit sticker save again and then put that sticker on the dispenser. Then you need to print stickers for both protocols and stick them on two controls slides and run. I admit its a little bit of a pain. Mark On Fri, Jun 18, 2010 at 9:41 AM, Mike Pence wrote: > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this in > "parallel" > > Mike > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents tested > in parallel with old lots? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using an > appropriate panel of control tissues.) > > Ellen Yee > _____ > > From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > To: Ellen Yee [mailto:eyee@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From BSullivan <@t> shorememorial.org Fri Jun 18 13:23:44 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Fri Jun 18 13:26:11 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: Message-ID: Regarding this question. This is my take on this. You always have to check Lot to Lot antibodies and make sure you have consistent results. We have a ledger where our new lots are recorded when received into the lab. There is also a place for all the necessary information about the antibody, the lot number , open date, Q.C. date and the antibody's expiration date. Once the antibody is opened it is Q.C'd on tissue that we have used in the past for control material. The control is checked and it is noted if the results are favorable or not. If there are favorable results it is put into production. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "McMahon, Loralee A" , Ellen u> Yee , Laurie Sent by: Colbert histonet-bounces@ ern.edu cc "histonet@lists.utsouthwestern.edu" 06/18/2010 01:47 Subject PM RE: [Histonet] New CAP question ANP.22760 I think that CAP means that you need to save the slide that you ran from the previous lot and compare it to the slide that you have stained with the new lot number. To see if they are sufficient diagnostic quality. Not put both lot numbers on the machine at the same time and then compare the slides? We run Dako machines and it would be tricky to put both numbers on the same machine. Although this is my interpretation. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpence@grhs.net] Sent: Friday, June 18, 2010 12:41 PM To: Ellen Yee; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 I don't think I can do this with the automated system we are currently using. Ventana. Does any other Ventana users know if you can do this in "parallel" Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Thursday, June 17, 2010 7:21 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _____ From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] To: Ellen Yee [mailto:eyee@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Jun 18 13:25:33 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Jun 18 13:26:31 2010 Subject: [Histonet] RE: IHC kits In-Reply-To: <443F5B475A9BF647AB962E834884EBAD278D1277E3@EX7CCRPW03V1.chop.edu> References: <3809C163DC1DA54AA534B3C7794D07B65A8FBDC0E5@ENHBGMBX01.PA.LCL>, <443F5B475A9BF647AB962E834884EBAD278D1277E3@EX7CCRPW03V1.chop.edu> Message-ID: You could try Biocare regents. I use many Biocare reagents. Or sign a contract with Dako to lock in your prices....... Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mauger, Joanne [MAUGER@email.chop.edu] Sent: Friday, June 18, 2010 1:59 PM To: Charles, Roger; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: IHC kits The Leica Bond detection kits are better than LSAB. They are barcoded for the Bondmax machine-not sure if they offer packaged another way. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger [rcharles@state.pa.us] Sent: Friday, June 18, 2010 10:38 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC kits Happy Friday to all, Dako has once again raised their prices to the point that I'm asked to find another IHC labeling kit that is cheaper. Could those doing IHC please recommend a kit that is as good as Dako's LSAB2? Thanks so much. Roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Fri Jun 18 13:28:38 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Jun 18 13:28:45 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: <24A4826E8EF0964D86BC5317306F58A542598BCCCE@mmc-mail.ad.mhsil.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3E50@is-e2k3.grhs.net> As I have found over the years the opinions and interpretation of CAP intent is differing. Everyone has how they see the question and think it to best be answered. I am not sure why it is not acceptable for the lab to run a control on the new lot and make sure it works and that is the QC/QA. The pathologist is the one that determines if the stain is acceptable for diagnosis. Some of the suggestions would be very time consuming and costly to do each new lot#. -----Original Message----- From: Vickroy, Jim [mailto:Vickroy.Jim@mhsil.com] Sent: Friday, June 18, 2010 1:06 PM To: Mark Tarango; Mike Pence Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 I'm kind of getting in on the middle of this but is anybody else doing this with prep kit stickers? I have not seen the new question but has anyone talked to CAP to get a clarification of what they mean on this question? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 12:27 PM To: Mike Pence Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 You'll have to use prep kit stickers and duplicate protocols to do it, but it is possible. You just need to copy the protocol and save it as another number, then change the primary antibody to a prep kit sticker save again and then put that sticker on the dispenser. Then you need to print stickers for both protocols and stick them on two controls slides and run. I admit its a little bit of a pain. Mark On Fri, Jun 18, 2010 at 9:41 AM, Mike Pence wrote: > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this > in "parallel" > > Mike > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents > tested in parallel with old lots? (NOTE: New lots of primary antibody > and detection system reagents must be compared to the previous lot > using an appropriate panel of control tissues.) > > Ellen Yee > _____ > > From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > To: Ellen Yee [mailto:eyee@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From marktarango <@t> gmail.com Fri Jun 18 13:46:53 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Jun 18 13:46:58 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: References: <20100618002037.0ab34f26@mail.dpmginc.com> <661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> Message-ID: That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the ?old? lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpassar@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < Loralee_Mcmahon@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran from > the previous lot and compare it to the slide that you have stained with the > new lot number. To see if they are sufficient diagnostic quality. Not put > both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpence@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this in > "parallel" > > Mike > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents tested > in parallel with old lots? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using an > appropriate panel of control tissues.) > > Ellen Yee > _____ > > From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > To: Ellen Yee [mailto:eyee@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tjasper <@t> copc.net Fri Jun 18 13:51:00 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Jun 18 13:51:05 2010 Subject: [Histonet] FW: test Message-ID: <90354A475B420441B2A0396E5008D49695E957@copc-sbs.COPC.local> test _____ From: Thomas Jasper Sent: Friday, June 18, 2010 11:17 AM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: test Thanks, checking on connection. tj From tjasper <@t> copc.net Fri Jun 18 14:38:07 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Jun 18 14:38:12 2010 Subject: [Histonet] New CAP question ANP.22760 References: <20100618002037.0ab34f26@mail.dpmginc.com> <661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> Message-ID: <90354A475B420441B2A0396E5008D49692BF6B@copc-sbs.COPC.local> Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 11:47 AM To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the "old" lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpassar@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < Loralee_Mcmahon@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran > from the previous lot and compare it to the slide that you have > stained with the new lot number. To see if they are sufficient > diagnostic quality. Not put both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpence@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this > in "parallel" > > Mike > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents > tested in parallel with old lots? (NOTE: New lots of primary antibody > and detection system reagents must be compared to the previous lot > using an appropriate panel of control tissues.) > > Ellen Yee > _____ > > From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > To: Ellen Yee [mailto:eyee@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Fri Jun 18 14:43:46 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Fri Jun 18 14:53:22 2010 Subject: [Histonet] FW: test In-Reply-To: <90354A475B420441B2A0396E5008D49695E957@copc-sbs.COPC.local> References: <90354A475B420441B2A0396E5008D49695E957@copc-sbs.COPC.local> Message-ID: <4C1BCC72.9030809@vneubert.com> re > test > > _____ > > From: Thomas Jasper > Sent: Friday, June 18, 2010 11:17 AM > To: 'histonet-bounces@lists.utsouthwestern.edu' > Subject: test > > > Thanks, checking on connection. > tj > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From boor <@t> email.cz Fri Jun 18 14:56:14 2010 From: boor <@t> email.cz (Peter Boor) Date: Fri Jun 18 14:56:21 2010 Subject: [Histonet] PPARalpha Message-ID: <000001cb0f20$4fe503d0$efaf0b70$@cz> Hi everyone, Does anyone has experience with PPARalpha staining in rats and mice (in kidneys)? Which antibodies work? I have tried the Abcam polyclonal rabbit (ab8934) Ab that is supposed to work, but it is not (tried methyl carnoyls solution, formalin, frozen sections and various Ag retrievals). I would be very grateful for any suggestions! Many thanks in advance, Peter P E T E R B O O R, MD, PhD Dpt. of Nephrology & Inst. of Pathology, RWTH University Aachen Pauwelstr. 30 52074 Aachen, Germany Tel.:+49 241 80 89670 E-Mail: boor@email.cz From Maxim_71 <@t> mail.ru Fri Jun 18 15:00:36 2010 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Fri Jun 18 14:59:32 2010 Subject: [Histonet] Breast Specimen handling Message-ID: <93959025.20100619000036@mail.ru> Kathy: After 48-72 hrs 10% NBF we wash our cassettes during 10-15 mins in tap water, then store it in 70% isopropyl alcohol (IPA) before processing. You can store tissues here as long as need. The IPA does not harden it as ethanol. We using as dehydrant also IPA. Sincerely, Maxim Peshkov, Taganrog, Russia. ---Original message--- > Date: Thu, 17 Jun 2010 13:24:22 -0400 > From: "Sara Baldwin/mhhcc.org" > Subject: [Histonet] Breast Specimen handling > To: Histo Net > Message-ID: > > > > Content-Type: text/plain; charset="ISO-8859-1" > > > Hi histonetters > > Was wondering if anyone saw the new gui > specimen handling ? I am sure you have.&nbs is > wondering is if you have a large specimen and do > tissue for processing what would the agent be > to apply specimen to stop penetration of the > formlalin before the 72 hour ti me arrives? Would > it be sodium chloride? Can someone help? > < Thanks > Pathology Supervisor > Kathy Baldwin, S Memorial Hospital and Health > Care&nbs [1]sbaldwin@m Ph 812-482-0210, 482-0216, > Fax 81 Pager 812-481-0897 > Confidential information, Authorized use only. > > References > > 1. 3D"mailto:sbaldwin@mhhcc.org" mailto:Maxim_71@mail.ru From mpence <@t> grhs.net Fri Jun 18 15:12:36 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Jun 18 15:12:53 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: <90354A475B420441B2A0396E5008D49692BF6B@copc-sbs.COPC.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3E51@is-e2k3.grhs.net> Tom I did notice that too and wondered just how long this person had been "out" of the working lab setting. This was something that was done back when IHC was done all manually. I think I will just take my chances with what I am doing now as acceptable and wait and see what the CAP inspector thinks or at least how they are dealing with this question! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Friday, June 18, 2010 2:38 PM To: Mark Tarango Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 11:47 AM To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the "old" lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpassar@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < Loralee_Mcmahon@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran > from the previous lot and compare it to the slide that you have > stained with the new lot number. To see if they are sufficient > diagnostic quality. Not put both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpence@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this > in "parallel" > > Mike > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents > tested in parallel with old lots? (NOTE: New lots of primary antibody > and detection system reagents must be compared to the previous lot > using an appropriate panel of control tissues.) > > Ellen Yee > _____ > > From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > To: Ellen Yee [mailto:eyee@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Jun 18 15:24:54 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Jun 18 15:25:09 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: <90354A475B420441B2A0396E5008D49692BF6B@copc-sbs.COPC.local> References: <20100618002037.0ab34f26@mail.dpmginc.com> <661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> <90354A475B420441B2A0396E5008D49692BF6B@copc-sbs.COPC.local> Message-ID: Hi Tom, I did notice. In my original question, I had said something like "is it possible that the Pathologists that gave the teleconference are somewhat out of touch with the realities of doing this in the lab?" Instead of the speakers writing back, I got her response. I was just glad that she did say it was acceptable (but not best practice) to compare against a previosly stained slide. In the teleconference he was very clear that he wanted the slides to be stained side-by-side. Mark On Fri, Jun 18, 2010 at 12:38 PM, Thomas Jasper wrote: > Mark, > > Did you notice the credentials from this CAP representative? MT with a > Blood Bank specialty I believe. What I glean from that is...more than > likely this person does not grasp the logistics of "contemporaneously" > staining identical Abs from separate lots. She also likely does not > understand the logistical application for detection and automation > either. > > I'm not trying to be overly critical of this person. I'm sure she is > quite intelligent and would not have the MT/SBB if she wasn't > intelligent. It comes down to a lack of understanding Anatomic > Pathology testing application re: automated IHC. I believe this is a > common problem in and out of CAP. Many lab directors and other folks in > positions of authority without AP/Histology/Cytology backgrounds seem to > believe that broad clinical lab modalities apply to Anatomic Path > scenarios. I used to refer to this in my former position as - "Trying > to put the yoke of clinical lab onto anatomic path." We are > laboratorians, but in many instances do not fit the general clinical lab > mold. > > It's unfortunate that CAP has put this person in the position to > respond. It is apparent to me that she's not grasping the particulars > here. She probably never will unless she decides to go into a working, > automated IHC "tissue" lab and take the time to ask questions and > understand (learn) what we're all about. > > Thanks, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services > Bend, OR 97701 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark > Tarango > Sent: Friday, June 18, 2010 11:47 AM > To: McMahon, Loralee A > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] New CAP question ANP.22760 > > That's what I thought at first too. It might be helpful to post this > letter that I got from the CAP about this. I tried to argue with them, > but this is the answer I got. > > > Dear Mark, > > Your questions were forwarded to me for response. > > > > During the Audio-conference, the idea of comparing a previously stained > slide (that had used the "old" lot) to one stained with the new lot was > deemed acceptable, but not optimal. Doing a simultaneous staining using > old and new lots, better demonstrates the performance characteristics of > the reagent. The reason parallel staining is considered best practice > is that all other variables, such as variations in the lot of detection > reagent or instrument function, are eliminated from consideration when > the slides are stained contemporaneously. > > > > The antibody "getting weak over time" should not happen to a significant > degree if the antibody is used within its expiration date. If the lab > is having this kind of trouble, it should look carefully at its storage > conditions. > > > > Demonstrating acceptable performance of the new lot, before being place > into service, is *required* for all accredited laboratories. > > > > To answer the last question, the key is to order the new reagent well > before you run out of the old lot so that the parallel stain can be > performed before the old lot is consumed. One multi-tissue slide control > slide would suffice to evaluate a primary antibody lot in most cases, > which helps to minimize the impact on the lab. > > > > I hope that this information is helpful. Thank you for your > participation in the Laboratory Accreditation Program. > > > > Sincerely, > > > > *Kathy Passarelli, MT(ASCP)SBB* > > *Technical Specialist* > > *Laboratory Accreditation Program* > > *College** of American** Pathologists* > > *Phone: 1-(800)-323-4040 ext 7486* > > *e-mail: **kpassar@cap.org* > > > > On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < > Loralee_Mcmahon@urmc.rochester.edu> wrote: > > > I think that CAP means that you need to save the slide that you ran > > from the previous lot and compare it to the slide that you have > > stained with the new lot number. To see if they are sufficient > > diagnostic quality. Not put both lot numbers on the machine at the > same time and then compare the > > slides? We run Dako machines and it would be tricky to put both > numbers on > > the same machine. > > > > Although this is my interpretation. > > > > Loralee McMahon, HTL (ASCP) > > Immunohistochemistry Supervisor > > Strong Memorial Hospital > > Department of Surgical Pathology > > (585) 275-7210 > > ________________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu [ > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > > mpence@grhs.net] > > Sent: Friday, June 18, 2010 12:41 PM > > To: Ellen Yee; Laurie Colbert > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] New CAP question ANP.22760 > > > > I don't think I can do this with the automated system we are currently > > > using. Ventana. Does any other Ventana users know if you can do this > > in "parallel" > > > > Mike > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > > Yee > > Sent: Thursday, June 17, 2010 7:21 PM > > To: Laurie Colbert > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] New CAP question ANP.22760 > > > > > > Sorry, I should have included it. > > > > ANP.22760 Are new lots of antibody and detection system reagents > > tested in parallel with old lots? (NOTE: New lots of primary antibody > > > and detection system reagents must be compared to the previous lot > > using an appropriate panel of control tissues.) > > > > Ellen Yee > > _____ > > > > From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > > To: Ellen Yee [mailto:eyee@dpmginc.com] > > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > > Subject: RE: [Histonet] New CAP question ANP.22760 > > > > Can you give us the wording of that question/checklist item? Laurie > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > > Yee > > Sent: Wednesday, June 16, 2010 10:10 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] New CAP question ANP.22760 > > > > How are IHC labs complying with this question? What is considered an > > appropriate panel of control tissues? What do you stain to test your > > detection systems? > > > > Ellen Yee > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From liz <@t> premierlab.com Fri Jun 18 15:29:10 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jun 18 15:29:14 2010 Subject: [Histonet] PPARalpha In-Reply-To: <000001cb0f20$4fe503d0$efaf0b70$@cz> Message-ID: We tried the ppar from abcam and was not to impressed by it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter Boor Sent: Friday, June 18, 2010 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PPARalpha Hi everyone, Does anyone has experience with PPARalpha staining in rats and mice (in kidneys)? Which antibodies work? I have tried the Abcam polyclonal rabbit (ab8934) Ab that is supposed to work, but it is not (tried methyl carnoyls solution, formalin, frozen sections and various Ag retrievals). I would be very grateful for any suggestions! Many thanks in advance, Peter P E T E R B O O R, MD, PhD Dpt. of Nephrology & Inst. of Pathology, RWTH University Aachen Pauwelstr. 30 52074 Aachen, Germany Tel.:+49 241 80 89670 E-Mail: boor@email.cz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Fri Jun 18 15:38:01 2010 From: annigyg <@t> gmail.com (annigyg@gmail.com) Date: Fri Jun 18 15:38:08 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: References: <20100618002037.0ab34f26@mail.dpmginc.com><661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> Message-ID: <378219058-1276893475-cardhu_decombobulator_blackberry.rim.net-1736743232-@bda242.bisx.produk.on.blackberry> Am following this IHC/CAP thread with great interest, but I have to ask...what is the origin of the word 'contemporaneously'? English is my mother tongue but this word is new to me- simultaneous and contemporary make sense but this 'amalgamation' is an abomination! Annie. Anyone? Empower your Business with BlackBerry? and Mobile Solutions from Etisalat -----Original Message----- From: Mark Tarango Sender: histonet-bounces@lists.utsouthwestern.edu Date: Fri, 18 Jun 2010 11:46:53 To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the ?old? lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpassar@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < Loralee_Mcmahon@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran from > the previous lot and compare it to the slide that you have stained with the > new lot number. To see if they are sufficient diagnostic quality. Not put > both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 >________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpence@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this in > "parallel" > > Mike > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents tested > in parallel with old lots? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using an > appropriate panel of control tissues.) > > Ellen Yee >_____ > > From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > To: Ellen Yee [mailto:eyee@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Fri Jun 18 16:04:08 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Jun 18 16:04:23 2010 Subject: [Histonet] New CAP question ANP.22760 Message-ID: <20100618140408.9e2d9aa830e8449a2412eb1e4f2f067e.61f342a68e.wbe@email04.secureserver.net> They're taking lessons from our former president of the United St ates!! =) Sarah Goebel, B.A., HT (ASCP) < Histotechnicia XBiotech USA Inc. < Austin, Texas 78744 < (512) -------- Original Message -------- Subject: Re: [Histonet] New CAP question ANP.22760 From: [1]annigyg@gmail.com Date: Fri, June 18, 2010 1:38 pm To: "Mark Tarango" <[2]marktaran <[3]histonet@lists.u Am following this IHC/CAP thread with great interest, but I have to ask...w English is my mother tongue but this word is new to me- simultaneous and co abomination! Annie. Anyone? Empower your Business with BlackBerry? and Mobile Solutions from Etisa -----Original Message----- From: Mark Tarango <[4]marktaran Sender: [5]hist Date: Fri, 18 Jun 2010 11:46:53 To: McMahon, Loralee A<[6]Loralee_Mcmahon@urmc.rochester.edu> Cc: [7]histonet@lists.u tsouthwestern.edu<[8]histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this lette that I got from the CAP about this. I tried to argue with them, but this i the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the ?old? lot) to one stained with the deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that< detection reagent or< consideration when the slides are< The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place int service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well befor you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would< which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation< Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: [9]**kpassar@cap.org* < On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < [11]Loralee_Mcmahon@urm > I think that CAP means that you need to save the slide that you ran fr > the previous lot and compare it to the slide that you have stained wit > new lot number. To see if they are sufficient diagnostic quality. No > both lot numbers on the machine at the same time and then compare the< both numb > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 >________________________________________ > From: [12]h > [13]histone Pence [ > [14]mpence@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: [15]histonet@l > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this i > "parallel" > > Mike > -----Original Message----- > From: [16]h > [[17]mailto:h Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: [18]histonet@li > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents test > in parallel with old lots? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using a > appropriate panel of control tissues.) > > Ellen Yee >_____ > > From: Laurie Colbert [[19]mailto:laurie.colbert@huntingtonhospital.com] > To: Ellen Yee [[20]mailto:eyee@dpmginc > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -----Original Message----- > From: [21]h > [[22]mailto:h Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: [23]histonet@li > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > >_______________________________________________ > Histonet mailing list > [24]Histonet@lists. > [25] > > > >_______________________________________________ > Histonet mailing list > [26]Histonet@lists. > [27] > > > >_______________________________________________ > Histonet mailing list > [28]Histonet@lists. > [29] >_______________________________________________ > Histonet mailing list > [30]Histonet@lists. > [31] > _______________________________________________ Histonet mailing list [32]Histonet@lists.utsou [33]http: _________________________________________________________________ _______________________________________________ Histonet mailing list [34]Histonet@lists.utsou [35]http: References 1. 3D"mailto://annigyg@gmail.com"/ 2. 3D"mailto://marktarango@gmail.com"/ 3. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 4. 3D"mailto://marktarango@gmail.com"/ 5. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 6. 3D"mailto://Loralee_Mcmahon@urmc.rocheste=/ 7. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 8. 3D"mailto://histonet@lists.utsouthwestern.=/ 9. 3D"mailto://**kpassar@cap.org"/ 10. 3D"mailto://kpassar@cap.org"/ 11. 3D"mailto://Loralee_Mcmahon@urmc.rochester.edu"/ 12. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 13. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 14. 3D"mailto://mpence@grhs.net"/ 15. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 16. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 17. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 18. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 19. 3D"mailto:laurie.colbert@huntingtonhosp 20. 3D"mailto:eyee@dpmginc.com" 21. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 22. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 23. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 24. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 25. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 26. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 27. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 28. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 29. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 30. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 31. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 32. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 33. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 34. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 35. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From contact <@t> excaliburpathology.com Fri Jun 18 16:32:08 2010 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Jun 18 16:32:15 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: <378219058-1276893475-cardhu_decombobulator_blackberry.rim.net-1736743232-@bda242.bisx.produk.on.blackberry> References: <20100618002037.0ab34f26@mail.dpmginc.com><661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> <378219058-1276893475-cardhu_decombobulator_blackberry.rim.net-1736743232-@bda242.bisx.produk.on.blackberry> Message-ID: <673460.78720.qm@web1101.biz.mail.sk1.yahoo.com> http://www.thefreedictionary.com/contemporaneously ________________________________ From: "annigyg@gmail.com" To: Mark Tarango ; Histonet Sent: Fri, June 18, 2010 3:38:01 PM Subject: Re: [Histonet] New CAP question ANP.22760 Am following this IHC/CAP thread with great interest, but I have to ask...what is the origin of the word 'contemporaneously'? English is my mother tongue but this word is new to me- simultaneous and contemporary make sense but this 'amalgamation' is an abomination! Annie. Anyone? Empower your Business with BlackBerry? and Mobile Solutions from Etisalat -----Original Message----- From: Mark Tarango Sender: histonet-bounces@lists.utsouthwestern.edu Date: Fri, 18 Jun 2010 11:46:53 To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too.? It might be helpful to post this letter that I got from the CAP about this.? I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the ?old? lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent.? The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date.? If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful.? Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail:? **kpassar@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < Loralee_Mcmahon@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran from > the previous lot and compare it to the slide that you have stained with the > new lot number.? To see if they are sufficient diagnostic quality.? Not put > both lot numbers on the machine at the same time and then compare the > slides?? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 >________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpence@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert >? Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this in > "parallel" > > Mike > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760? Are new lots of antibody and detection system reagents tested > in parallel with old lots?? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using an > appropriate panel of control tissues.) > > Ellen Yee >_____ > >? From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > To: Ellen Yee [mailto:eyee@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mabosso <@t> unipathllc.com Fri Jun 18 16:51:00 2010 From: mabosso <@t> unipathllc.com (Mary Abosso) Date: Fri Jun 18 16:52:13 2010 Subject: [Histonet] New CAP question ANP.22760 References: <661949901A768E4F9CC16D8AF8F2838C017A3E51@is-e2k3.grhs.net> Message-ID: <43A451981FF6634795BE83B1B5494D631BE10E@exchange.unipathllc.corp> One thing I noticed was: "*Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* and then everything else became clear. Mary Abosso Denver, CO ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mike Pence Sent: Fri 6/18/2010 2:12 PM To: Thomas Jasper; Mark Tarango Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Tom I did notice that too and wondered just how long this person had been "out" of the working lab setting. This was something that was done back when IHC was done all manually. I think I will just take my chances with what I am doing now as acceptable and wait and see what the CAP inspector thinks or at least how they are dealing with this question! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Friday, June 18, 2010 2:38 PM To: Mark Tarango Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 11:47 AM To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the "old" lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpassar@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < Loralee_Mcmahon@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran > from the previous lot and compare it to the slide that you have > stained with the new lot number. To see if they are sufficient > diagnostic quality. Not put both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpence@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this > in "parallel" > > Mike > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents > tested in parallel with old lots? (NOTE: New lots of primary antibody > and detection system reagents must be compared to the previous lot > using an appropriate panel of control tissues.) > > Ellen Yee > _____ > > From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > To: Ellen Yee [mailto:eyee@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Elliott <@t> hli.ubc.ca Fri Jun 18 16:55:32 2010 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Fri Jun 18 16:56:23 2010 Subject: [Histonet] beta-myosin heavy chain (Myh7) antibody In-Reply-To: References: Message-ID: <4C1B88E4020000D60004ACFE@mail.mrl.ubc.ca> Does anyone know of a supplier for an antibody against beta-myosin heavy chain (Myh7) that works in mouse tissue and have experience working with it? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From dogsloveus2 <@t> yahoo.com Fri Jun 18 19:21:59 2010 From: dogsloveus2 <@t> yahoo.com (dogsloveus2) Date: Fri Jun 18 19:22:02 2010 Subject: [Histonet] Re: Histonet Digest, Vol 79, Issue 22 Message-ID: <297367.27653.qm@web59905.mail.ac4.yahoo.com> From dogsloveus2 <@t> yahoo.com Fri Jun 18 19:21:59 2010 From: dogsloveus2 <@t> yahoo.com (dogsloveus2) Date: Fri Jun 18 19:22:03 2010 Subject: [Histonet] Re: Histonet Digest, Vol 79, Issue 22 Message-ID: <609794.76897.qm@web59907.mail.ac4.yahoo.com> From dogsloveus2 <@t> yahoo.com Fri Jun 18 19:22:00 2010 From: dogsloveus2 <@t> yahoo.com (dogsloveus2) Date: Fri Jun 18 19:22:06 2010 Subject: [Histonet] Re: Histonet Digest, Vol 79, Issue 22 Message-ID: <222521.70245.qm@web59901.mail.ac4.yahoo.com> From tjay30 <@t> yahoo.com Sat Jun 19 12:22:25 2010 From: tjay30 <@t> yahoo.com (Timothy Jay) Date: Sat Jun 19 12:22:29 2010 Subject: [Histonet] P/T Job Opening Message-ID: <607567.39163.qm@web34305.mail.mud.yahoo.com> I have 3+ P/T openings for certified HTs in Folsom, Santa Rosa, and Torrance, California. All jobs are with GI groups and are brand new labs. Very competitive pay, excellent working conditions, and very flexible hours. If you are looking for a change?of scenery or just need extra work please consider working for one of these?great new labs. ?Please submit resumes to Timothy Jay at tjay30@yahoo.com?c/o Pillar Consulting. Thank you. From lguidot <@t> ameripath.com Sat Jun 19 14:42:20 2010 From: lguidot <@t> ameripath.com (Guidot, Lucie) Date: Sat Jun 19 14:42:31 2010 Subject: [Histonet] Histology Supervisor Position in Kansas City Message-ID: <301E08EC3961144EA578DEFE6EBFA7E6049A17A87F@MWNMAIL00.ameripath.local> Our Kansas City, Missouri laboratory, which produces ~500 blocks per night and has an extensive library of IHC and special stains, has an opening for a histology supervisor. We are looking for an experienced histology supervisor with expertise in IHC and well-developed teambuilding skills. Please see our website (www.ameripath.com) to apply and/or contact me if you are interested. Lucie Guidot AmeriPath Kansas City | Operations Manager | 10330 Hickman Mills Drive | Kansas City, MO 64137 USA | phone +1.816.412.7008| fax +816.763.7536| mobile +1.602.319.9609| lguidot@AmeriPath.com | www.AmeriPath.com P Please think about resource conservation before you print this message CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From raj <@t> bluemarble.net Sat Jun 19 21:38:25 2010 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Sat Jun 19 21:38:51 2010 Subject: [Histonet] Grossing tech Message-ID: <25F882AEAF5C487F90C093C701CF7FA4@CHURCH> What are the requirements for a person to gross? This is for a derm lab. Thanks raj From Rcartun <@t> harthosp.org Sun Jun 20 14:57:27 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Jun 20 15:02:44 2010 Subject: [Histonet] release of tissues In-Reply-To: References: Message-ID: <4C1E3A66.7400.0077.1@harthosp.org> We do not release any tissue to patients or family members. If they want the specimen they have to go through a funeral home. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 6/17/2010 2:32 PM >>> Hi all, How would you handle a request from a surgeon, f= or release of gallstones or any tissue, to a patient? Assuming a 'Rele= ase of Tissue' request form, indicating the biohazard staus of the tissue, is signed by the patient, is this commom practice? Thanks for your responses, Mary Sue CONFID= ENTIALITY NOTICE: This e-mail and attachments, if any, may contain= confidential information which is privileged and protected from disclosure= by Federal and State confidentiality laws, rules or regulations. This= e-mail and attachments, if any, are intended for the designated ad= dressee only. If you are not the designated addressee, you are hereby = notified that any disclosure, copying, or distribution of this e-ma= il and its attachments, if any, may be unlawful and may subject you= to legal consequences. If you have received this e-mail and its attachmen= ts in error, please contact the St. Mary's Hospital Helpdesk at (301) 4= 75-6166 and delete the e-mail and its attachments from your computer. Than= k you for your attention. Please consider the environment: = do you really need to print this email? CONFIDENTIALITY NOTICE: This = e-mail and attachments, if any, may contain confidential information which = is privileged and protected from disclosure by Federal and State confidenti= ality laws, rules or regulations. This e-mail and attachments, if any, are= intended for the designated addressee only. If you are not the designated addressee, you are hereby notified that any disclosure, copying, or distri= bution of this e-mail and its attachments, if any, may be unlawful and may = subject you to legal consequences. If you have received this e-mail and it= s attachments in error, please contact the St. Mary's Hospital Helpdesk at = (301) 475-6166 and delete the e-mail and its attachments from your computer= . Thank you for your attention. Please consider the environment: do you really need to print this email? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhersch2 <@t> illinois.edu Sun Jun 20 20:50:15 2010 From: bhersch2 <@t> illinois.edu (Brad Herschler) Date: Sun Jun 20 20:50:39 2010 Subject: [Histonet] Disposal of MMA & BPO Waste Message-ID: We use an MMA embedding technique similar to that used for histology in our lab. We sent a request to our campus Chemical Waste Management for the MMA waste mixture to be disposed of, but they are refusing because they say the mixture is too hazardous. The mixture contains 1gram of benzoyl peroxide (BPO) per 100mL of MMA. Chemical Waste Management informed us that MMA in the presence of BPO is highly reactive. We would like to know: for histology, how is MMA + BPO waste normally disposed of? Thanks From ratliffjack <@t> hotmail.com Mon Jun 21 06:35:06 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Jun 21 06:35:10 2010 Subject: [Histonet] Disposal of MMA & BPO Waste In-Reply-To: References: Message-ID: The simple thing to do is just polymerize the waste. I am not sure of the volume of waste that you mention in your message, but I routinely polymerize my MMA waste and then safely discard for glass disposal. 1) I fill a 2 - 2.5 L glass amber bottle (usually bottle that MMA comes in originally) with approximately 1.5 L of MMA waste 2) add 10-15 g of a benzoyl peroxide based catalyst (Perkadox 16) 3) cap and mix throughly (shaking or swirling) until catalyst is dissolved into solution 4) place the glass container in the hood on a disposable piece of lab bench paper (keep a clean air space free around container) 5) UN CAP BOTTLE (very important step here as polymerization can be explosive due to the exothermic reation of the solution) 6) allow to sit freely for 2-3 days until solution is completely polymerized 7) after polymerization, turn bottle on side and allow to vent for 1-2 days (may or may not be a necessary step) 8) cap bottle and discard with broken glass Hope this helps and please ask more questions if you need further clarification or explanation. Jack > From: bhersch2@illinois.edu > Date: Sun, 20 Jun 2010 20:50:15 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Disposal of MMA & BPO Waste > > We use an MMA embedding technique similar to that used for histology in our > lab. We sent a request to our campus Chemical Waste Management for the MMA > waste mixture to be disposed of, but they are refusing because they say the > mixture is too hazardous. The mixture contains 1gram of benzoyl peroxide > (BPO) per 100mL of MMA. Chemical Waste Management informed us that MMA in > the presence of BPO is highly reactive. > > We would like to know: for histology, how is MMA + BPO waste normally > disposed of? > > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2 From misbaked <@t> hotmail.com Mon Jun 21 08:05:01 2010 From: misbaked <@t> hotmail.com (MICHELLE BAKER) Date: Mon Jun 21 08:05:04 2010 Subject: [Histonet] Amyloidosis control tissue Message-ID: Hi - Does anyone know where to buy amyloidosis control tissue? Thanks- Missy Baker _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3 From DKnutson <@t> primecare.org Mon Jun 21 08:30:18 2010 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Mon Jun 21 08:30:26 2010 Subject: [Histonet] Pneumo Message-ID: <1E0E2B14C709174B8AC2BE0AE7F768338FEB35E32A@EXCHANGE2K7.staprimecare.org> Good Morning All, Does anyone know where I can purchase pneumo controls of fluid such as BAL? We are experiencing an increase of orders on BAL for pneumocystis. How are the rest of you handling such requests? Do you run a cellblock control or do you have direct smear controls? Thank you for any feedback you can give me. Have a nice day! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From HornHV <@t> archildrens.org Mon Jun 21 08:51:37 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Jun 21 08:51:48 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: References: <20100618002037.0ab34f26@mail.dpmginc.com><661949901A768E4F9CC16D8AF8F2838C017A3E4E@is-e2k3.grhs.net> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D838E4@EMAIL.archildrens.org> So CAP answered the pathology question with a person who is a med tech with a specialty in blood banking... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 1:47 PM To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the "old" lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpassar@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < Loralee_Mcmahon@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran from > the previous lot and compare it to the slide that you have stained with the > new lot number. To see if they are sufficient diagnostic quality. Not put > both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpence@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this in > "parallel" > > Mike > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents tested > in parallel with old lots? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using an > appropriate panel of control tissues.) > > Ellen Yee > _____ > > From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > To: Ellen Yee [mailto:eyee@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. 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From sgoebel <@t> xbiotech.com Mon Jun 21 08:53:00 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Jun 21 08:53:08 2010 Subject: [Histonet] Amyloidosis control tissue Message-ID: <20100621065300.9e2d9aa830e8449a2412eb1e4f2f067e.83144ab2bd.wbe@email04.secureserver.net> American Mastertech Sarah Goebel, B.A., HT (ASCP) H XBiotech USA Inc. 8201 East Ri -------- Original Message -------- Subject: [Histonet] Amyloidosis control tissue From: MICHELLE BAKER <[1]misbaked Date: Mon, June 21, 2010 6:05 am To: <[2]histonet@lis Hi - Does anyone know where to buy amyloidosis control tissue? Thanks- Missy Baker _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox.< [3]http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326:: T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3_________________________________ _____ Histonet mailing list [4]Histonet@lists.utsou [5]http: References 1. 3D"mailto://misbaked@hotmail.com"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326:: 4. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From mucram11 <@t> comcast.net Mon Jun 21 09:17:59 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Jun 21 09:18:02 2010 Subject: [Histonet] Disposal of MMA & BPO Waste In-Reply-To: Message-ID: <1004280759.117195.1277129879508.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I was at UPENN Vet School at the time I used this method.??We sometimes had 4 to 6 bottles under the hood to polymerize.? We would use Nalgene 1 liter bottles about 2/3s full and allow them to polymerize under the hood.? We always sat the bottles in large Tri-Pour disposable beakers in case the bottle crack or overflowed during polymerization and then through them out in the normal waste.? If we had straight MMA we added some BPO to allow it to polymerize completely.? Larger amounts would be far more difficult to control the reaction in and contain it.? Pam Marcum UAMS ----- Original Message ----- From: "Brad Herschler " To: histonet @lists. utsouthwestern . edu Sent: Sunday, June 20, 2010 8:50:15 PM Subject: [ Histonet ] Disposal of MMA & BPO Waste We use an MMA embedding technique similar to that used for histology in our lab. ?We sent a request to our campus Chemical Waste Management for the MMA waste mixture to be disposed of, but they are refusing because they say the mixture is too hazardous. ?The mixture contains 1gram of benzoyl peroxide ( BPO ) per 100mL of MMA . ?Chemical Waste Management informed us that MMA in the presence of BPO is highly reactive. We would like to know: for histology, how is MMA + BPO waste normally disposed of? Thanks _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet From rjbuesa <@t> yahoo.com Mon Jun 21 10:13:56 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 21 10:14:03 2010 Subject: [Histonet] Pneumo In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F768338FEB35E32A@EXCHANGE2K7.staprimecare.org> Message-ID: <177598.88653.qm@web65708.mail.ac4.yahoo.com> I used an autopsy lung infested control. Perhaps you could try to?a similar source for your controls. Ren? J. --- On Mon, 6/21/10, Knutson, Deanne wrote: From: Knutson, Deanne Subject: [Histonet] Pneumo To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, June 21, 2010, 9:30 AM Good Morning All, Does anyone know where I can purchase pneumo controls of fluid such as BAL?? We are experiencing an increase of orders on BAL for pneumocystis.? How are the rest of you handling such requests?? Do you run a cellblock control or do you have direct smear controls?? Thank you for any feedback you can give me.? Have a nice day! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Mon Jun 21 11:33:42 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Jun 21 11:33:45 2010 Subject: [Histonet] Block disposal In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F768338FEB35E32A@EXCHANGE2K7.staprimecare.org> Message-ID: <2B7A4A4F7B4C44048CD6B849FCDECA63@hopperPC> Would blocks be considered biohazard waste for purposes of disposal? I have blocks stored in an off-site facility and am preparing to dispose of the blocks. Can I simply allow the storage place dispose of the blocks in "normal" trash, or would I need to have them disposed of as biohazard trash? On a separate but sort of related issue, does anyone use the Adlex crystals to dispose of their formalin? If yes, how do you dispose of the jellied formalin, in normal trash or in biohazard trash? I know the mfr says you can put it in the normal trash, but does anyone trust this?? ;o) By the way, I am in Florida, if that makes any difference in the answers! THANKS! Michelle From Valerie.Hannen <@t> parrishmed.com Mon Jun 21 12:08:18 2010 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Jun 21 12:10:51 2010 Subject: [Histonet] Amyloidosis control tissue References: Message-ID: <5680DA93771F0C48954CC8D38425E72401AD03C2@ISMAIL.parrishmed.local> Missy, It is my understanding that at this time there are no control tissues available. I myself have tried to get some from various places and have been told it is very difficult for the vendors to come by them. Valerie Hannen ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of MICHELLE BAKER Sent: Mon 6/21/2010 9:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amyloidosis control tissue Hi - Does anyone know where to buy amyloidosis control tissue? Thanks- Missy Baker _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From asmith <@t> mail.barry.edu Mon Jun 21 12:24:52 2010 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Mon Jun 21 12:26:39 2010 Subject: [Histonet] Disposal of MMA & BPO Waste In-Reply-To: References: Message-ID: This is what comes of letting people with little or no training in chemistry call themselves chemical waste mangers. Yes, methyl methacrylate is highly reactive when mixed with 1% benzoyl peroxide: it forms a hard, brittle polymer. Pour your left over methyl methacrylate and benzoyl peroxide into a disposable weighing boat and heat it overnight at 60 C and over another night at 80 C. The resultant polymer is commercially sold as "Plexiglass;" it can be safely disposed of as ordinary trash. A much higher concentration of benzoyl peroxide (10% or more) could explode when heated. Chemical potential depends on concentration. -Allen A. Smith, M.A. (chemistry), Ph.D. (anatomy) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brad Herschler Sent: Sunday, June 20, 2010 9:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of MMA & BPO Waste We use an MMA embedding technique similar to that used for histology in our lab. We sent a request to our campus Chemical Waste Management for the MMA waste mixture to be disposed of, but they are refusing because they say the mixture is too hazardous. The mixture contains 1gram of benzoyl peroxide (BPO) per 100mL of MMA. Chemical Waste Management informed us that MMA in the presence of BPO is highly reactive. We would like to know: for histology, how is MMA + BPO waste normally disposed of? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy_schmitt <@t> pa-ucl.com Mon Jun 21 13:16:07 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Jun 21 13:16:13 2010 Subject: [Histonet] New CAP question ANP.22760 - continued...... In-Reply-To: <20100621174224.7C666964A2@mail.pa-ucl.com> References: <20100621174224.7C666964A2@mail.pa-ucl.com> Message-ID: <737BD0BF52F0744B96B74B61756AC0644166C332B3@hestia.ad.pa-ucl.com> Hi to all- I hate to kick this dog any more than it already has been, but I still would like to know what is considered an appropriate panel of control tissues - and is it in a microarray or sausage? What do you stain to test your detection systems? Thanks much- Nancy Schmitt HT(ASCP)MLT(CSMLS) Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From settembr <@t> umdnj.edu Mon Jun 21 13:38:40 2010 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Jun 21 13:39:03 2010 Subject: [Histonet] New CAP question ANP.22760 - continued...... Message-ID: Nancy, Pose that question to CAP @ accred@cap.org They are very good at returning email questions and you'll have it from them and in writing. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Nancy Schmitt 06/21/10 2:16 PM >>> Hi to all- I hate to kick this dog any more than it already has been, but I still would like to know what is considered an appropriate panel of control tissues - and is it in a microarray or sausage? What do you stain to test your detection systems? Thanks much- Nancy Schmitt HT(ASCP)MLT(CSMLS) Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Mon Jun 21 14:02:20 2010 From: jstaruk <@t> masshistology.com (jstaruk) Date: Mon Jun 21 14:02:34 2010 Subject: [Histonet] New England Shandon Rep. In-Reply-To: <24A4826E8EF0964D86BC5317306F58A542598BCC11@mmc-mail.ad.mhsil.com> Message-ID: <1442898EA17E496180BD958AC908C0D4@JimPC> Does anyone know if there is a Rep that sells Shandon equipment in New England? We're looking to purchase a slide printer and a search through the Histonet archives identifies this brand as one of the better slide printers available. Thank you Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com From frankhlau <@t> gmail.com Mon Jun 21 14:11:06 2010 From: frankhlau <@t> gmail.com (Frank Lau) Date: Mon Jun 21 14:11:31 2010 Subject: [Histonet] Autofluorescence in murine white adpose tissue cryosections Message-ID: Hi everyone, I'm trying to cryosection mouse white adipose tissue. The end-goal is to immunostain these sections. Unfortunately, all of my sections are autofluorescing heavily. My protocol is: 1. Harvest tissue and rinse in PBS 2. Fix for 2 hours in 4% PFA at 4 Celsius 3. Wash 3x with PBS for 5 minutes per wash 4. Incubate in 30% sucrose overnight at 4C 5. Wash with O.C.T. 6. Incubate in O.C.T. overnight at 4C 7. Embed and cryosection 8. Bring to room temp in PBS 9. Check sections under the microscope (no mounting, no staining yet) I thought perhaps I was over-fixing the tissues, so I dropped the 4% PFA fix time to 15 minutes. I don't see any notable difference in the degree of autofluorescence. Thanks in advance for any help you can provide. Frank From akbitting <@t> geisinger.edu Mon Jun 21 14:15:22 2010 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Jun 21 14:15:45 2010 Subject: [Histonet] Block disposal In-Reply-To: <2B7A4A4F7B4C44048CD6B849FCDECA63@hopperPC> References: <1E0E2B14C709174B8AC2BE0AE7F768338FEB35E32A@EXCHANGE2K7.staprimecare.org> <2B7A4A4F7B4C44048CD6B849FCDECA63@hopperPC> Message-ID: <4C1F820F.2B7F.00C9.0@geisinger.edu> They are classified as Pathological Waste. You should see what your state's rules are regarding Pathological Waste. >>> 6/21/2010 12:33 PM >>> Would blocks be considered biohazard waste for purposes of disposal? I have blocks stored in an off-site facility and am preparing to dispose of the blocks. Can I simply allow the storage place dispose of the blocks in "normal" trash, or would I need to have them disposed of as biohazard trash? On a separate but sort of related issue, does anyone use the Adlex crystals to dispose of their formalin? If yes, how do you dispose of the jellied formalin, in normal trash or in biohazard trash? I know the mfr says you can put it in the normal trash, but does anyone trust this?? ;o) By the way, I am in Florida, if that makes any difference in the answers! THANKS! Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Lynne.Bell <@t> cvmc.org Mon Jun 21 14:34:07 2010 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Mon Jun 21 14:34:12 2010 Subject: [Histonet] New England Shandon Rep. In-Reply-To: <1442898EA17E496180BD958AC908C0D4@JimPC> Message-ID: Hi Jim, Darlene Alleman with ThermoFisher Scientific is my Shandon rep. She lives in New Hampshire. Her phone numbers are (800) 522-7270, ext 449 and email is darlene.alleman@thermofisher.com. She is an excellent rep and I highly recommend her. Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From laurie.colbert <@t> huntingtonhospital.com Mon Jun 21 14:47:51 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Jun 21 14:48:00 2010 Subject: [Histonet] New CAP question ANP.22760 - continued...... Message-ID: <57BE698966D5C54EAE8612E8941D768308F25575@EXCHANGE3.huntingtonhospital.com> Nancy, If you email CAP and get a response, please post it for all of us to see. Thanks! Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Settembre Sent: Monday, June 21, 2010 11:39 AM To: histonet@lists.utsouthwestern.edu; Nancy Schmitt Subject: Re: [Histonet] New CAP question ANP.22760 - continued...... Nancy, Pose that question to CAP @ accred@cap.org They are very good at returning email questions and you'll have it from them and in writing. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Nancy Schmitt 06/21/10 2:16 PM >>> Hi to all- I hate to kick this dog any more than it already has been, but I still would like to know what is considered an appropriate panel of control tissues - and is it in a microarray or sausage? What do you stain to test your detection systems? Thanks much- Nancy Schmitt HT(ASCP)MLT(CSMLS) Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Mon Jun 21 15:37:15 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Jun 21 15:37:20 2010 Subject: [Histonet] Disposal of MMA & BPO Waste In-Reply-To: References: , Message-ID: I would just like to say that I personally disagree with the heating of the MMA @ 60C and then subsequently @ 80C. The fumes that will be generated are toxic enough without heat so heating of the solution is a recipie for disaster! Also, it was not mentioned if the weigh boat was aluminum or plastic or if the oven was placed in a hood or well ventilated or isolated room. If you simply add 10-15 grams of catalyst to approximately 1000 - 1500 mL to the waste MMA solution and leave in the hood overnight or even over the weekend to polymerize with the cap off, you will be safe. This is approximately 1 - 1.5% w/w concentration of catalyst to solution, well below the 10% as suggested in the comment below. You could even do as Pam suggests and place the container in a bucket as an extra precaution (without a lid) in case the glass was to break. Just did 2 bottles over the weekend and I have been doing this safely for the past 13 + years. Jack > From: asmith@mail.barry.edu > To: bhersch2@illinois.edu > Date: Mon, 21 Jun 2010 17:24:52 +0000 > Subject: RE: [Histonet] Disposal of MMA & BPO Waste > CC: histonet@lists.utsouthwestern.edu > > This is what comes of letting people with little or no training in chemistry call themselves chemical waste mangers. > Yes, methyl methacrylate is highly reactive when mixed with 1% benzoyl peroxide: it forms a hard, brittle polymer. Pour your left over methyl methacrylate and benzoyl peroxide into a disposable weighing boat and heat it overnight at 60 C and over another night at 80 C. The resultant polymer is commercially sold as "Plexiglass;" it can be safely disposed of as ordinary trash. > A much higher concentration of benzoyl peroxide (10% or more) could explode when heated. Chemical potential depends on concentration. > -Allen A. Smith, M.A. (chemistry), Ph.D. (anatomy) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brad Herschler > Sent: Sunday, June 20, 2010 9:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Disposal of MMA & BPO Waste > > We use an MMA embedding technique similar to that used for histology in our > lab. We sent a request to our campus Chemical Waste Management for the MMA > waste mixture to be disposed of, but they are refusing because they say the > mixture is too hazardous. The mixture contains 1gram of benzoyl peroxide > (BPO) per 100mL of MMA. Chemical Waste Management informed us that MMA in > the presence of BPO is highly reactive. > > We would like to know: for histology, how is MMA + BPO waste normally > disposed of? > > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From cbarone <@t> NEMOURS.ORG Mon Jun 21 15:37:32 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon Jun 21 15:37:37 2010 Subject: [Histonet] HT or HTL certified? Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7DBC@wlmmsx01.nemours.org> Nemours is in-search of an HT or HTL certified, or eligible - tech for Histotechnology Core Facility, Wilmington Delaware. Position 50% clinical/50% research. Background in enzyme histochemistry, IF and IHC required. Molecular histology, LMD or Imaging a plus. contact: cbarone@nemours.org From histotech90 <@t> cox.net Mon Jun 21 17:52:17 2010 From: histotech90 <@t> cox.net (jill) Date: Mon Jun 21 17:52:28 2010 Subject: [Histonet] Looking for Histology job in Seattle area Message-ID: <5DB29AE98ECA4750B116BC78DECA852D@OwnerPC> Hello All, I am looking to relocate to the Seattle Washington area and would like to know if anyone knows of any openings? I am HT ascp and have 17 years experience along with 5 years management. Will consider all positions, thanks in advance!! Please reply to this email. From DianaRip1 <@t> aol.com Mon Jun 21 18:43:20 2010 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Mon Jun 21 18:43:46 2010 Subject: [Histonet] Help! In need of positive Gram Control Message-ID: <26338.611bde74.39515318@aol.com> Help! We are in need of positive Gram Control Blocks if anyone has any extra they are willing to part with. I have lots of Fungus, Pneumocystis and HPV tissue blocks to trade. Diana Ripley John Muir Histology Concord Campus 2540 East Street Concord, CA 94520 From POWELL_SA <@t> mercer.edu Mon Jun 21 21:08:42 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Jun 21 21:11:17 2010 Subject: [Histonet] Help! In need of positive Gram Control In-Reply-To: <26338.611bde74.39515318@aol.com> References: <26338.611bde74.39515318@aol.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE2268F09C1DE@MERCERMAIL.MercerU.local> Go to your local drive in mart and buy you a Slim Jim, cut it in thin slices and you will have all the Gram controls you can use. You will never eat another Slim Jim. If you don't know what they are, they are sort of like a summer sausage, only thinner and guys love them. They are found usually right beside the check out register. Shirley ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DianaRip1@aol.com [DianaRip1@aol.com] Sent: Monday, June 21, 2010 7:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help! In need of positive Gram Control Help! We are in need of positive Gram Control Blocks if anyone has any extra they are willing to part with. I have lots of Fungus, Pneumocystis and HPV tissue blocks to trade. Diana Ripley John Muir Histology Concord Campus 2540 East Street Concord, CA 94520 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfrmd1 <@t> gmail.com Mon Jun 21 21:43:47 2010 From: cfrmd1 <@t> gmail.com (Carlos Rodriguez, MD) Date: Mon Jun 21 21:43:51 2010 Subject: [Histonet] Histotech job opportunity in Scottsdale, AZ Message-ID: We are a private practice dermatology group in Scottsdale with an in-house dermpath lab, and we will be in need of a part-time histotech in the coming months. If interested, please reply to this message and attach a CV and/or some basic info about your credentials and work experience. Thank you. Carlos Rodriguez, MD From carolina.soekmadji <@t> qut.edu.au Tue Jun 22 01:34:38 2010 From: carolina.soekmadji <@t> qut.edu.au (Carolina Soekmadji) Date: Tue Jun 22 01:34:47 2010 Subject: [Histonet] tumour, lymph node and prostate preservation for histology Message-ID: <60495E34E2CF58448A44DE567C6FAF240BC2A38DC3@QUTEXMBX03.qut.edu.au> Hi, I am new to histology and have some very basic questions. I hope somebody out there willing to help me.. I want to isolate tumours from mice and preserve it for histology . I heard that there is different method on how best preserving different organs/ tissue. Could any of you please advise me a method on how to best preserve tumour ? Also if there a method to preserve lymph node and prostate? Thank you very much. Best regards, Carolina Carolina Soekmadji Postdoctoral Research Fellow Australian Prostate Cancer Research Centre - Queensland | Institute of Health and Biomedical Innovation | t: 07 3176 7428 (APCRC - Princess Alexandra Hospital) t: 07 3138 6286 (IHBI - QUT Kelvin Grove) mobile: +61 423 111 807 f: 07 3176 7440 e: carolina.soekmadji@qut.edu.au From AnthonyH <@t> chw.edu.au Tue Jun 22 02:13:37 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jun 22 02:13:51 2010 Subject: [Histonet] tumour,lymph node and prostate preservation for histology In-Reply-To: <60495E34E2CF58448A44DE567C6FAF240BC2A38DC3@QUTEXMBX03.qut.edu.au> Message-ID: Carolina, It will depend on what you want to do to the tissue after removal. For Histology, placing the tissue in 10% phosphate buffered formalin is good for morphology, special staains, immunohistochemistry and in situ hybridisation. I have been able to extract RNA and DNA from formalin fixed, paraffin embedded tissues that have been stored at room temperature for 40 years! (and others have also shown this). Contact me for further details. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carolina Soekmadji Sent: Tuesday, 22 June 2010 4:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tumour,lymph node and prostate preservation for histology Hi, I am new to histology and have some very basic questions. I hope somebody out there willing to help me.. I want to isolate tumours from mice and preserve it for histology . I heard that there is different method on how best preserving different organs/ tissue. Could any of you please advise me a method on how to best preserve tumour ? Also if there a method to preserve lymph node and prostate? Thank you very much. Best regards, Carolina Carolina Soekmadji Postdoctoral Research Fellow Australian Prostate Cancer Research Centre - Queensland | Institute of Health and Biomedical Innovation | t: 07 3176 7428 (APCRC - Princess Alexandra Hospital) t: 07 3138 6286 (IHBI - QUT Kelvin Grove) mobile: +61 423 111 807 f: 07 3176 7440 e: carolina.soekmadji@qut.edu.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From JCBRITTON <@t> Cheshire-Med.COM Tue Jun 22 05:09:42 2010 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Tue Jun 22 05:10:20 2010 Subject: [Histonet] Help! In need of positive Gram Control In-Reply-To: <26338.611bde74.39515318@aol.com> References: <26338.611bde74.39515318@aol.com> Message-ID: Have you tried a Slim Jim? They have gram positive and negative rods in them. Regardless, I still enjoy eating them once and a while! Josie Britton Ht Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DianaRip1@aol.com Sent: Monday, June 21, 2010 7:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help! In need of positive Gram Control Help! We are in need of positive Gram Control Blocks if anyone has any extra they are willing to part with. I have lots of Fungus, Pneumocystis and HPV tissue blocks to trade. Diana Ripley John Muir Histology Concord Campus 2540 East Street Concord, CA 94520 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From JCBRITTON <@t> Cheshire-Med.COM Tue Jun 22 05:22:56 2010 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Tue Jun 22 05:23:01 2010 Subject: [Histonet] Breezy lab/flyaway ribbons! Message-ID: We have been having trouble with big breeze blowing our precious ribbons out of our hands while cutting. We would like a door on the Histology lab to cut down on the breeze of people walking by through the hall. Our facilities want to find out what other people are doing to stop this problem. We also have air ducts blowing down from above, which is not helping the problem. We would like as many labs solutions as possible. Our facilities have come up with all these crazy barriers that we would have to move to walk around when we need to put our racks on the stainer, answer timers, print more slides, use the oven, etc... Any input would be appreciated! Breezy girls, Josie Britton and Chris Braaten Cheshire Medical Center Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From godsgalnow <@t> aol.com Tue Jun 22 06:11:26 2010 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Jun 22 06:11:32 2010 Subject: [Histonet] Histotechs needed in Long Island Message-ID: <400326378-1277205086-cardhu_decombobulator_blackberry.rim.net-1519889657-@bda2106.bisx.prod.on.blackberry> We have several openings for our fast growing lab. We need Registrations Associates as well. The open positions are: 1 Full time tech with EM experience, 1 full time tech with IHC experience, 2 full time day shift positions and 1 full time overnight position. Excellent opportunity for NYS licensed techs. Sent from my Verizon Wireless BlackBerry From patpxs <@t> gmail.com Tue Jun 22 06:36:20 2010 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Tue Jun 22 06:36:25 2010 Subject: [Histonet] Breezy lab/flyaway ribbons! In-Reply-To: References: Message-ID: Hi Josie and Chris, I've worked in labs with similar problems. Breezes are nice outdoors but cause havoc with ribbons. If you can't get them to put in a door (which you'll probably have to lock since folks will walk in, shut the door and cause a breeze that way) then how about those fuzzy cubicle walls? The ones that are used in prairie dog farms (I mean open office situations ;-) ). Also, you'll need to install some type of baffle or other item that hangs from the air ducts and deflects the air across the ceiling instead of straight down. Good luck! Paula :-) On Tue, Jun 22, 2010 at 6:22 AM, Josie Britton wrote: > We have been having trouble with big breeze blowing our precious ribbons > out of our hands while cutting. We would like a door on the Histology > lab to cut down on the breeze of people walking by through the hall. > Our facilities want to find out what other people are doing to stop this > problem. We also have air ducts blowing down from above, which is not > helping the problem. We would like as many labs solutions as possible. > Our facilities have come up with all these crazy barriers that we would > have to move to walk around when we need to put our racks on the > stainer, answer timers, print more slides, use the oven, etc... > > > > Any input would be appreciated! > > > > Breezy girls, > > > > Josie Britton and Chris Braaten > > Cheshire Medical Center > > Keene, NH 03431 > > > CONFIDENTIALITY NOTICE: This electronic message, including any attachments, > is for the sole use of the intended recipients and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by electronic mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab From trathborne <@t> somerset-healthcare.com Tue Jun 22 07:49:56 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jun 22 07:50:03 2010 Subject: [Histonet] Breezy lab/flyaway ribbons! In-Reply-To: Message-ID: For the ceiling vents, you could ask to have deflectors installed. The ones we have are made of stainless, are slightly larger than the vent, and are suspended about 6"-8" below the opening. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Josie Britton Sent: Tuesday, June 22, 2010 6:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breezy lab/flyaway ribbons! We have been having trouble with big breeze blowing our precious ribbons out of our hands while cutting. We would like a door on the Histology lab to cut down on the breeze of people walking by through the hall. Our facilities want to find out what other people are doing to stop this problem. We also have air ducts blowing down from above, which is not helping the problem. We would like as many labs solutions as possible. Our facilities have come up with all these crazy barriers that we would have to move to walk around when we need to put our racks on the stainer, answer timers, print more slides, use the oven, etc... Any input would be appreciated! Breezy girls, Josie Britton and Chris Braaten Cheshire Medical Center Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From arsenn <@t> hsh.org Tue Jun 22 08:13:00 2010 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Tue Jun 22 08:39:07 2010 Subject: [Histonet] Hpylori/Giemsa Message-ID: Histoland, For those of you who order your H-pylori/Giemsa controls: Who do you use? There were 2 companies that we were using for years, and all of a sudden, there's no bugs in the tissue-many, many boxes were without bugs! And they told my boss that the IHC controls are not 'guaranteed' to show positive for H-pylori and/or Giemsa. We are trying to cut our own, but finding that we need to use more as controls than we actually have-so we're running out frequently. Any help would be greatly appreciated! Thanks so much! HT Amy in Camp Hill, PA www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. From cgill <@t> marylandgeneral.org Tue Jun 22 09:16:14 2010 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Tue Jun 22 09:16:26 2010 Subject: [Histonet] Help! In need of positive Gram Control In-Reply-To: References: <26338.611bde74.39515318@aol.com> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9EAC@MDGEN-EXCH1.marylandgeneral.org> You have got to be kidding!! That's hysterical. So process a slim jim and you have Gram - and + controls. If you're serious I'm trying it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Josie Britton Sent: Tuesday, June 22, 2010 6:10 AM To: DianaRip1@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Have you tried a Slim Jim? They have gram positive and negative rods in them. Regardless, I still enjoy eating them once and a while! Josie Britton Ht Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DianaRip1@aol.com Sent: Monday, June 21, 2010 7:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help! In need of positive Gram Control Help! We are in need of positive Gram Control Blocks if anyone has any extra they are willing to part with. I have lots of Fungus, Pneumocystis and HPV tissue blocks to trade. Diana Ripley John Muir Histology Concord Campus 2540 East Street Concord, CA 94520 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Tue Jun 22 09:33:06 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Jun 22 09:33:52 2010 Subject: [Histonet] Help! In need of positive Gram Control In-Reply-To: <087A9911BBAFDE4B8151CB148586E2C23A9EAC@MDGEN-EXCH1.marylandgeneral.org> References: <26338.611bde74.39515318@aol.com> <087A9911BBAFDE4B8151CB148586E2C23A9EAC@MDGEN-EXCH1.marylandgeneral.org> Message-ID: <4C20C9A2.5060104@pathology.washington.edu> What a waste of a good Slim Jim. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 6/22/2010 7:16 AM, Gill, Caula A. wrote: > You have got to be kidding!! That's hysterical. So process a slim jim > and you have > Gram - and + controls. If you're serious I'm trying it. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:10 AM > To: DianaRip1@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > > > > > > Have you tried a Slim Jim? They have gram positive and negative rods in > > them. Regardless, I still enjoy eating them once and a while! > > > > > > > > Josie Britton Ht > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > DianaRip1@aol.com > > Sent: Monday, June 21, 2010 7:43 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Help! In need of positive Gram Control > > > > > > > > Help! We are in need of positive Gram Control Blocks if anyone has any > > > > extra they are willing to part with. I have lots of Fungus, > > Pneumocystis and > > > > HPV tissue blocks to trade. > > > > > > > > Diana Ripley > > > > John Muir Histology > > > > Concord Campus > > > > 2540 East Street > > > > Concord, CA 94520 > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jackie.O'Connor <@t> abbott.com Tue Jun 22 10:05:44 2010 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jun 22 10:06:19 2010 Subject: [Histonet] Help! In need of positive Gram Control In-Reply-To: <4C20C9A2.5060104@pathology.washington.edu> References: <26338.611bde74.39515318@aol.com> <087A9911BBAFDE4B8151CB148586E2C23A9EAC@MDGEN-EXCH1.marylandgeneral.org> <4C20C9A2.5060104@pathology.washington.edu> Message-ID: A good ol' hot appendix works great. Not as good as a Slim Jim, tho. From histotech <@t> imagesbyhopper.com Tue Jun 22 10:10:29 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Jun 22 10:10:34 2010 Subject: [Histonet] Breezy lab/flyaway ribbons! In-Reply-To: Message-ID: <2AA992355E8E4CBF91361F0DBE9E4E05@hopperPC> We have a similar situation, but I have shown my techs how to 1) wet the brush they use to take the ribbon off the knife and 2) to roll/curl the ribbon over the damp end of the brush and 3) hover our hand over the top of the ribbon while moving it (to reduce the breeze) from the microtome to the waterbath. I learned this technique when I first started in Histology, as we had a choice, a HOT room with no AC and no wind, or learn how to deal with the breeze and don't sweat! I opted for dealing with the wind. Now, it's second nature and it matters not whether there is wind or not, I still use the same motion for tranfer of the ribbon! Good luck with your situation. :o) Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, June 22, 2010 8:50 AM To: Josie Britton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! For the ceiling vents, you could ask to have deflectors installed. The ones we have are made of stainless, are slightly larger than the vent, and are suspended about 6"-8" below the opening. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Josie Britton Sent: Tuesday, June 22, 2010 6:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breezy lab/flyaway ribbons! We have been having trouble with big breeze blowing our precious ribbons out of our hands while cutting. We would like a door on the Histology lab to cut down on the breeze of people walking by through the hall. Our facilities want to find out what other people are doing to stop this problem. We also have air ducts blowing down from above, which is not helping the problem. We would like as many labs solutions as possible. Our facilities have come up with all these crazy barriers that we would have to move to walk around when we need to put our racks on the stainer, answer timers, print more slides, use the oven, etc... Any input would be appreciated! Breezy girls, Josie Britton and Chris Braaten Cheshire Medical Center Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: 06/22/10 06:36:00 From rsrichmond <@t> gmail.com Tue Jun 22 11:19:43 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Jun 22 11:19:55 2010 Subject: [Histonet] Re: Amyloidosis control tissue Message-ID: It's always been difficult to get amyloid control tissue. Amyloidosis is a rather rare disease in the USA, and some cases simply do not stain well. I've asked this question on Histonet several times and no one has ever responded: Amyloidosis (of the AA type I think) is rather easily produced in mice and other small experimental animals. Why can't this tissue be available as an amyloid control? Seems that some enterprising person could make a little money this way. Bob Richmond Samurai Pathologist Knoxville TN From histology <@t> medsurgpath.com Tue Jun 22 11:30:00 2010 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Tue Jun 22 11:30:10 2010 Subject: [Histonet] Grossing tech Message-ID: We are currently hiring for a grossing tech (in Portland, OR-reply to email if interested), we do more than derm and we use the CLIA regulations: TESTING PERSONNEL (42 CFR 493 1489) 1. Licensed MD, DO or DPM. 2. Doctorate, master's, or bachelor's in laboratory science. 3. Education and training equivalent to an associate degree in a laboratory science or medical laboratory technology that includes at least 60 semester hours including 24 semester hrs of medical lab technology and at least 3 months training in each specialty in which high complexity testing is performed; or, 60 semester hrs including 24 hrs of science that includes 6 hrs chemistry, 6 hrs biology, and 12 hrs chemistry, biology or, medical lab tech in any combination and laboratory training that includes either: completion of a clinical lab training program and at least 3 months training in each specialty in which high complexity testing is performed Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 What are the requirements for a person to gross? This is for a derm lab. Thanks raj _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu From sweething63 <@t> msn.com Tue Jun 22 13:07:32 2010 From: sweething63 <@t> msn.com (R J VAZQUEZ) Date: Tue Jun 22 13:07:39 2010 Subject: [Histonet] Breezy lab/flyaway ribbons! In-Reply-To: <2AA992355E8E4CBF91361F0DBE9E4E05@hopperPC> References: , <2AA992355E8E4CBF91361F0DBE9E4E05@hopperPC> Message-ID: When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways. Robyn Vazquez > From: histotech@imagesbyhopper.com > To: trathborne@somerset-healthcare.com; JCBRITTON@Cheshire-Med.COM; histonet@lists.utsouthwestern.edu > Date: Tue, 22 Jun 2010 11:10:29 -0400 > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > CC: > > We have a similar situation, but I have shown my techs how to 1) wet the > brush they use to take the ribbon off the knife and 2) to roll/curl the > ribbon over the damp end of the brush and 3) hover our hand over the top of > the ribbon while moving it (to reduce the breeze) from the microtome to the > waterbath. > > I learned this technique when I first started in Histology, as we had a > choice, a HOT room with no AC and no wind, or learn how to deal with the > breeze and don't sweat! I opted for dealing with the wind. Now, it's > second nature and it matters not whether there is wind or not, I still use > the same motion for tranfer of the ribbon! > > Good luck with your situation. :o) > > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, > Toni > Sent: Tuesday, June 22, 2010 8:50 AM > To: Josie Britton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > > > For the ceiling vents, you could ask to have deflectors installed. The ones > we have are made of stainless, are slightly larger than the vent, and are > suspended about 6"-8" below the opening. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Josie Britton > Sent: Tuesday, June 22, 2010 6:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breezy lab/flyaway ribbons! > > > We have been having trouble with big breeze blowing our precious ribbons > > out of our hands while cutting. We would like a door on the Histology > > lab to cut down on the breeze of people walking by through the hall. > > Our facilities want to find out what other people are doing to stop this > > problem. We also have air ducts blowing down from above, which is not > > helping the problem. We would like as many labs solutions as possible. > > Our facilities have come up with all these crazy barriers that we would > > have to move to walk around when we need to put our racks on the > > stainer, answer timers, print more slides, use the oven, etc... > > > > > > > > Any input would be appreciated! > > > > > > > > Breezy girls, > > > > > > > > Josie Britton and Chris Braaten > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any attachments, > is for the sole use of the intended recipients and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by electronic mail and destroy all copies of the original > message. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error by > e-mail or you may call Somerset Medical Center's computer Help Desk at > 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: 06/22/10 > 06:36:00 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Tue Jun 22 13:47:33 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Jun 22 13:47:39 2010 Subject: [Histonet] slim jims Message-ID: Do you put the slim jims in formalin and then process them or just put them in the processor? Margaret From cgill <@t> marylandgeneral.org Tue Jun 22 14:41:41 2010 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Tue Jun 22 14:41:48 2010 Subject: [Histonet] Breezy lab/flyaway ribbons! In-Reply-To: References: , <2AA992355E8E4CBF91361F0DBE9E4E05@hopperPC> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9EAD@MDGEN-EXCH1.marylandgeneral.org> Ditto, I use a small brush that I got from the craft store and prior to picking up the ribbon I wet the brush and slip it under my ribbon end closest to the blade and presto no flyaways. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R J VAZQUEZ Sent: Tuesday, June 22, 2010 2:08 PM To: histotech@imagesbyhopper.com; trathborne@somerset-healthcare.com; jcbritton@cheshire-med.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways. Robyn Vazquez > From: histotech@imagesbyhopper.com > To: trathborne@somerset-healthcare.com; JCBRITTON@Cheshire-Med.COM; > histonet@lists.utsouthwestern.edu > Date: Tue, 22 Jun 2010 11:10:29 -0400 > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > CC: > > We have a similar situation, but I have shown my techs how to 1) wet > the brush they use to take the ribbon off the knife and 2) to > roll/curl the ribbon over the damp end of the brush and 3) hover our > hand over the top of the ribbon while moving it (to reduce the breeze) > from the microtome to the waterbath. > > I learned this technique when I first started in Histology, as we had > a choice, a HOT room with no AC and no wind, or learn how to deal with > the breeze and don't sweat! I opted for dealing with the wind. Now, > it's second nature and it matters not whether there is wind or not, I > still use the same motion for tranfer of the ribbon! > > Good luck with your situation. :o) > > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Rathborne, Toni > Sent: Tuesday, June 22, 2010 8:50 AM > To: Josie Britton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > > > For the ceiling vents, you could ask to have deflectors installed. The > ones we have are made of stainless, are slightly larger than the vent, > and are suspended about 6"-8" below the opening. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breezy lab/flyaway ribbons! > > > We have been having trouble with big breeze blowing our precious > ribbons > > out of our hands while cutting. We would like a door on the Histology > > lab to cut down on the breeze of people walking by through the hall. > > Our facilities want to find out what other people are doing to stop > this > > problem. We also have air ducts blowing down from above, which is not > > helping the problem. We would like as many labs solutions as possible. > > Our facilities have come up with all these crazy barriers that we > would > > have to move to walk around when we need to put our racks on the > > stainer, answer timers, print more slides, use the oven, etc... > > > > > > > > Any input would be appreciated! > > > > > > > > Breezy girls, > > > > > > > > Josie Britton and Chris Braaten > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail > and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical > Center and are intended only for the addressee. The information > contained in this message is confidential and may contain privileged, > confidential, proprietary and/or trade secret information entitled to > protection and/or exemption from disclosure under applicable law. > Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you > are not the addressee, please promptly delete this message and notify > the sender of the delivery error by e-mail or you may call Somerset > Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: > 06/22/10 06:36:00 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Tue Jun 22 14:52:37 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Tue Jun 22 14:52:57 2010 Subject: [Histonet] Re: Autofluorescence in murine white adipose tissue cryosections Message-ID: Dear Frank, Good luck with ridding your samples of autofluorescence. Fat is always very brightly fluorescent and I suspect it might even be so without any aldehyde fixation. First I wondered how successful you'd be using Sudan Black B, knowing it is a fat stain, and knowing that it's been published for this purpose. So you might try just that and see how that works. In addition you could try using a combination of UV irradiation and the Sudan Black B and see if the combination works for you. They use paraffin sections in this reference, but it might work as well with cryosections. http://www.microscopyu.com/references/pdfs/Viegas_etal_Eur_J_Histochem-51-59-2007.pdf Good luck, and do report back if you find a solution to this dilemma! Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From histonet.nospam <@t> vneubert.com Tue Jun 22 14:58:39 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Tue Jun 22 14:58:47 2010 Subject: [Histonet] slim jims In-Reply-To: References: Message-ID: <4C2115EF.7030404@vneubert.com> I just found "a partially eaten Slim Jim snack" picture on wikipedia... Is it Friday again? :-> > Do you put the slim jims in formalin and then process them or just put them in the processor? > Margaret > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TJJ <@t> stowers.org Tue Jun 22 15:02:44 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Tue Jun 22 15:02:51 2010 Subject: [Histonet] X-gal staining troubleshooting Message-ID: Hi all, I have a question about whole mount x-gal staining. Do any of you have experience with your samples turning brown after incubation with the x-gal? If yes, do you know what causes this and how to keep it from happening? Thanks in advance, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From mward <@t> wfubmc.edu Tue Jun 22 15:10:12 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Jun 22 15:10:57 2010 Subject: [Histonet] Glut1 antibody Message-ID: <61135F0455D33347B5AAE209B903A30433DEBA4A@EXCHVS2.medctr.ad.wfubmc.edu> I have been using anti-human Glut-1 from Dako for several years but when I went to order it I was told it was discontinued. Does anyone have a suggestion of where I can locate this antibody? Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 From LSebree <@t> uwhealth.org Tue Jun 22 15:28:50 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jun 22 15:28:57 2010 Subject: [Histonet] Breezy lab/flyaway ribbons! In-Reply-To: <087A9911BBAFDE4B8151CB148586E2C23A9EAD@MDGEN-EXCH1.marylandgeneral.org> References: , <2AA992355E8E4CBF91361F0DBE9E4E05@hopperPC> <087A9911BBAFDE4B8151CB148586E2C23A9EAD@MDGEN-EXCH1.marylandgeneral.org> Message-ID: <8C023B4AB999614BA4791BAEB26E2738399EDC@UWHC-MAIL01.uwhis.hosp.wisc.edu> I do that too using either a wet brush or applicator stick but sometimes the breeze is more like a gale force wind breaking my ribbon apart to then adhere to everything but the surface of my waterbath! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gill, Caula A. Sent: Tuesday, June 22, 2010 2:42 PM To: R J VAZQUEZ; histotech@imagesbyhopper.com; trathborne@somerset-healthcare.com; jcbritton@cheshire-med.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! Ditto, I use a small brush that I got from the craft store and prior to picking up the ribbon I wet the brush and slip it under my ribbon end closest to the blade and presto no flyaways. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R J VAZQUEZ Sent: Tuesday, June 22, 2010 2:08 PM To: histotech@imagesbyhopper.com; trathborne@somerset-healthcare.com; jcbritton@cheshire-med.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways. Robyn Vazquez > From: histotech@imagesbyhopper.com > To: trathborne@somerset-healthcare.com; JCBRITTON@Cheshire-Med.COM; > histonet@lists.utsouthwestern.edu > Date: Tue, 22 Jun 2010 11:10:29 -0400 > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > CC: > > We have a similar situation, but I have shown my techs how to 1) wet > the brush they use to take the ribbon off the knife and 2) to > roll/curl the ribbon over the damp end of the brush and 3) hover our > hand over the top of the ribbon while moving it (to reduce the breeze) > from the microtome to the waterbath. > > I learned this technique when I first started in Histology, as we had > a choice, a HOT room with no AC and no wind, or learn how to deal with > the breeze and don't sweat! I opted for dealing with the wind. Now, > it's second nature and it matters not whether there is wind or not, I > still use the same motion for tranfer of the ribbon! > > Good luck with your situation. :o) > > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Rathborne, Toni > Sent: Tuesday, June 22, 2010 8:50 AM > To: Josie Britton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > > > For the ceiling vents, you could ask to have deflectors installed. The > ones we have are made of stainless, are slightly larger than the vent, > and are suspended about 6"-8" below the opening. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breezy lab/flyaway ribbons! > > > We have been having trouble with big breeze blowing our precious > ribbons > > out of our hands while cutting. We would like a door on the Histology > > lab to cut down on the breeze of people walking by through the hall. > > Our facilities want to find out what other people are doing to stop > this > > problem. We also have air ducts blowing down from above, which is not > > helping the problem. We would like as many labs solutions as possible. > > Our facilities have come up with all these crazy barriers that we > would > > have to move to walk around when we need to put our racks on the > > stainer, answer timers, print more slides, use the oven, etc... > > > > > > > > Any input would be appreciated! > > > > > > > > Breezy girls, > > > > > > > > Josie Britton and Chris Braaten > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail > and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical > Center and are intended only for the addressee. The information > contained in this message is confidential and may contain privileged, > confidential, proprietary and/or trade secret information entitled to > protection and/or exemption from disclosure under applicable law. > Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you > are not the addressee, please promptly delete this message and notify > the sender of the delivery error by e-mail or you may call Somerset > Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: > 06/22/10 06:36:00 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Jun 22 15:50:06 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jun 22 15:50:12 2010 Subject: [Histonet] slim jims In-Reply-To: References: Message-ID: if by "processor" you mean "my mouth", yes. the formalin is well, just a formality. HA! Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Tue, Jun 22, 2010 at 2:47 PM, Perry, Margaret wrote: > Do you put the slim jims in formalin and then process them or just put them > in the processor? > Margaret > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Sandra.Harrison3 <@t> va.gov Tue Jun 22 16:21:51 2010 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Tue Jun 22 16:22:07 2010 Subject: [Histonet] IHC for Legionella? Message-ID: Does anyone know of an IHC antibody for Legionella? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 From Rhonda.Hartz <@t> saskatoonhealthregion.ca Tue Jun 22 16:55:07 2010 From: Rhonda.Hartz <@t> saskatoonhealthregion.ca (Hartz, Rhonda SktnHR) Date: Tue Jun 22 17:03:54 2010 Subject: [Histonet] (no subject) Message-ID: Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca From Rcartun <@t> harthosp.org Tue Jun 22 17:31:49 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jun 22 17:31:56 2010 Subject: [Histonet] IHC for Legionella? In-Reply-To: References: Message-ID: <4C210194.7400.0077.1@harthosp.org> I offer IHC for Legionella in my laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Harrison, Sandra C." 6/22/2010 5:21 PM >>> Does anyone know of an IHC antibody for Legionella? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue Jun 22 17:52:34 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Jun 22 17:52:40 2010 Subject: [Histonet] Help! In need of positive Gram Control In-Reply-To: <26338.611bde74.39515318@aol.com> References: <26338.611bde74.39515318@aol.com> Message-ID: Slim Jim works great. This is what we used to use at AFIP. Just slice it and process it the same as your regular tissue. Jay A. Lundgren, M.S., HTL (ASCP) Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Jun 22 17:57:21 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jun 22 17:57:32 2010 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: We used to keep them for a week in a bag. If there were no problems at the end of the week we disposed of them "Hartz, Rhonda SktnHR" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/22/2010 03:24 PM To cc Subject [Histonet] (no subject) Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mia.woodruff <@t> qut.edu.au Tue Jun 22 18:06:14 2010 From: mia.woodruff <@t> qut.edu.au (Mia Woodruff) Date: Tue Jun 22 18:06:26 2010 Subject: [Histonet] Oven for paraffin slide drying Message-ID: Hello all, I need to purchase an oven for drying slides, on a very limited budget. I came across a "food dehydrator" which looks like an oven and has drawers which are the perfect size for the slide holders to fit into and it can reach a temperature of 60C - and it costs about 1/8 of the price of a normal oven. Can anyone foresee any issues in me using this for drying paraffin slides? As far as I can tell there is a low air flow inside and the temp can be set up to 60 degrees so it seems perfect? Any comments? Thanks Mia From rsrichmond <@t> gmail.com Tue Jun 22 18:34:09 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Jun 22 18:34:14 2010 Subject: [Histonet] Re: Help! In need of positive Gram Control (Jackie M O'Connor) Message-ID: I agree - acute appendicitis with rupture and an abscess provides an abundance of gram positive and gram negative bacteria, and such cases are common. I think that actual clinical tissue is better than controls faked with reference organisms. Tissue gram stains are greatly overrated. They don't work nearly as well as smears do. If you want to see bacteria in tissue sections, stain them with Giemsa or toluidine blue. Bob Richmond Samurai Pathologist Knoxville TN From talulahgosh <@t> gmail.com Tue Jun 22 19:16:41 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jun 22 19:16:44 2010 Subject: [Histonet] Oven for paraffin slide drying In-Reply-To: References: Message-ID: Do slide warmers cost too much? I don't know how much they cost, though, maybe an oven is cheaper. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Tue, Jun 22, 2010 at 7:06 PM, Mia Woodruff wrote: > Hello all, > > I need to purchase an oven for drying slides, on a very limited budget. I came across a "food dehydrator" which looks like an oven and has drawers which are the perfect size for the slide holders to fit into and it can reach a temperature of 60C - and it costs about 1/8 of the price of a normal oven. > Can anyone foresee any issues in me using this for drying paraffin slides? As far as I can tell there is a low air flow inside and the temp can be set up to 60 degrees so it seems perfect? Any comments? > > Thanks > Mia > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kjgada <@t> gmail.com Tue Jun 22 20:01:14 2010 From: kjgada <@t> gmail.com (Komal Gada) Date: Tue Jun 22 20:01:18 2010 Subject: [Histonet] Re: Amyloidosis control tissue In-Reply-To: References: Message-ID: American mastertech On Tue, Jun 22, 2010 at 12:19 PM, Robert Richmond wrote: > It's always been difficult to get amyloid control tissue. Amyloidosis > is a rather rare disease in the USA, and some cases simply do not > stain well. > > I've asked this question on Histonet several times and no one has ever > responded: Amyloidosis (of the AA type I think) is rather easily > produced in mice and other small experimental animals. Why can't this > tissue be available as an amyloid control? > > Seems that some enterprising person could make a little money this way. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From conniegrubaugh <@t> hotmail.com Tue Jun 22 20:06:14 2010 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Tue Jun 22 20:06:28 2010 Subject: [Histonet] Help! In need of positive Gram Control In-Reply-To: <087A9911BBAFDE4B8151CB148586E2C23A9EAC@MDGEN-EXCH1.marylandgeneral.org> References: <26338.611bde74.39515318@aol.com>, , <087A9911BBAFDE4B8151CB148586E2C23A9EAC@MDGEN-EXCH1.marylandgeneral.org> Message-ID: Tried the slim jim and all of my doctors did not like it. Don't waste your time. Connie G. > Date: Tue, 22 Jun 2010 10:16:14 -0400 > From: cgill@marylandgeneral.org > To: JCBRITTON@Cheshire-Med.COM; DianaRip1@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > CC: > > You have got to be kidding!! That's hysterical. So process a slim jim > and you have > Gram - and + controls. If you're serious I'm trying it. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:10 AM > To: DianaRip1@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > > > > > > Have you tried a Slim Jim? They have gram positive and negative rods in > > them. Regardless, I still enjoy eating them once and a while! > > > > > > > > Josie Britton Ht > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > DianaRip1@aol.com > > Sent: Monday, June 21, 2010 7:43 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Help! In need of positive Gram Control > > > > > > > > Help! We are in need of positive Gram Control Blocks if anyone has any > > > > extra they are willing to part with. I have lots of Fungus, > > Pneumocystis and > > > > HPV tissue blocks to trade. > > > > > > > > Diana Ripley > > > > John Muir Histology > > > > Concord Campus > > > > 2540 East Street > > > > Concord, CA 94520 > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From JMyers1 <@t> aol.com Tue Jun 22 20:50:42 2010 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Tue Jun 22 20:51:23 2010 Subject: [Histonet] New CAP question ANP.22760 Message-ID: <7cdd9.6a7042fa.3952c272@aol.com> Tom: As much as I agree with your acknowledgment that its seems a bit odd for the CAP to have a blood-banker responding to AP-related issue, I'm actually not surprised. The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time, and they wonder why IHC isn't expected to follow the same requirements as chemistry, immunology, etc. -- IHC is, after all, an awful lot like ELISA. And rightfully so, because IHC is, under CLIA (which supersedes CAP), considered highly-complex, non-waived testing -- and is, therefore, subject to the same Quality Systems regulations (in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. Could it be that, because AP produces qualitative results that are interpreted by a pathologist and CP produces quantitative results that are interpreted by an analyzer, we somehow think that CLIA rules don't apply to IHC? I certainly don't have the answer to that, but it make me wonder what the future holds. As witnessed by some of the newest CAP 'standards' (including the question in question...no pun intended), e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens must be tested, and where 10 of the positives must be weakly positive -- an acknowledgment that validation specimens must be carefully selected in order to obtain appropriate results), it certainly doesn't appear that the regulation of IHC testing is going to become more relaxed. Joe Myers, M.S., CT(ASCP) ------------------------------ Message: 12 Date: Fri, 18 Jun 2010 12:38:07 -0700 From: "Thomas Jasper" Subject: RE: [Histonet] New CAP question ANP.22760 To: "Mark Tarango" Cc: _histonet@lists.utsouthwestern.edu_ (mailto:histonet@lists.utsouthwestern.edu) Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 From Susan.Walzer <@t> HCAHealthcare.com Wed Jun 23 02:14:47 2010 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Wed Jun 23 02:14:59 2010 Subject: [Histonet] Help! In need of positive Gram Control In-Reply-To: References: <26338.611bde74.39515318@aol.com>, , <087A9911BBAFDE4B8151CB148586E2C23A9EAC@MDGEN-EXCH1.marylandgeneral.org> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AF9784613@FWDCWPMSGCMS09.hca.corpad.net> Take some fresh tissue (we use umbilical cord) to micro and let them incubate in for a few days in gram + and/or negative. Then fix it and you will have good controls. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: Tuesday, June 22, 2010 9:06 PM To: cgill@marylandgeneral.org; jcbritton@cheshire-med.com; dianarip1@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Tried the slim jim and all of my doctors did not like it. Don't waste your time. Connie G. > Date: Tue, 22 Jun 2010 10:16:14 -0400 > From: cgill@marylandgeneral.org > To: JCBRITTON@Cheshire-Med.COM; DianaRip1@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > CC: > > You have got to be kidding!! That's hysterical. So process a slim jim > and you have > Gram - and + controls. If you're serious I'm trying it. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:10 AM > To: DianaRip1@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > > > > > > Have you tried a Slim Jim? They have gram positive and negative rods in > > them. Regardless, I still enjoy eating them once and a while! > > > > > > > > Josie Britton Ht > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > DianaRip1@aol.com > > Sent: Monday, June 21, 2010 7:43 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Help! In need of positive Gram Control > > > > > > > > Help! We are in need of positive Gram Control Blocks if anyone has any > > > > extra they are willing to part with. I have lots of Fungus, > > Pneumocystis and > > > > HPV tissue blocks to trade. > > > > > > > > Diana Ripley > > > > John Muir Histology > > > > Concord Campus > > > > 2540 East Street > > > > Concord, CA 94520 > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From W.E.J.Hoekert <@t> olvg.nl Wed Jun 23 03:24:22 2010 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Wed Jun 23 03:32:48 2010 Subject: [Histonet] Glut1 antibody References: <61135F0455D33347B5AAE209B903A30433DEBA4A@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <1190CB05C44B13409483514729C2FC360C0ADF@PAIT42.olvg.nl> We switched to Neomarkers (polyclonal) (RB 078-A). Willem Hoekert OLVG, Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Martha Ward Verzonden: di 22-6-2010 22:10 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Glut1 antibody I have been using anti-human Glut-1 from Dako for several years but when I went to order it I was told it was discontinued. Does anyone have a suggestion of where I can locate this antibody? Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From mtitford <@t> aol.com Wed Jun 23 07:25:33 2010 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Jun 23 07:25:48 2010 Subject: [Histonet] Retaining empty jars Message-ID: <8CCE0E64F6A9C4A-FCC-6F33@Webmail-d119.sysops.aol.com> Rhonda Hartz asks if anyone else retains empty jars after grossing. We too retain our empty jars after grossing for about a month. We keep them in a red biohazard trash bag. It pays dividends. If once the case has been signed out the physician/surgeon complains that they removed more biopsies than we listed in the report, or submitted more jars, etc, we can go back and check. On rare occasions we have found tiny pieces of tissue in the jars, or hidden in the grooves of the lid. These enquiries most often happen with tiny pieces of tissue, of course, like GI biopsies. We have the physicians write on the label on the jar what is inside it (like everyone else I guess) as well as listing it on the surgical pathology slip. You can also go back and check what they wrote down. Afterwards the jars are incinerated as they have all that HIPPA related stuff on the labels. Michael Titford USA Pathology Mobile AL USA From rfields <@t> gidocs.net Wed Jun 23 08:08:49 2010 From: rfields <@t> gidocs.net (Rosa Fields) Date: Wed Jun 23 08:09:22 2010 Subject: [Histonet] Retaining empty jars References: <8CCE0E64F6A9C4A-FCC-6F33@Webmail-d119.sysops.aol.com> Message-ID: <07732CE52EC3174AB891DE1C62DB4D8FC7B81F@GIEXCHANGE.gidocs.net> We also retain ours; for about 2 weeks. I have plastic bins labeled Monday 1/ Monday 2.. ect.. that way I don't have to search through all of them if I have a question about a case. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Wednesday, June 23, 2010 7:26 AM To: Histonet@pathology.swmed.edu Subject: [Histonet] Retaining empty jars Rhonda Hartz asks if anyone else retains empty jars after grossing. We too retain our empty jars after grossing for about a month. We keep them in a red biohazard trash bag. It pays dividends. If once the case has been signed out the physician/surgeon complains that they removed more biopsies than we listed in the report, or submitted more jars, etc, we can go back and check. On rare occasions we have found tiny pieces of tissue in the jars, or hidden in the grooves of the lid. These enquiries most often happen with tiny pieces of tissue, of course, like GI biopsies. We have the physicians write on the label on the jar what is inside it (like everyone else I guess) as well as listing it on the surgical pathology slip. You can also go back and check what they wrote down. Afterwards the jars are incinerated as they have all that HIPPA related stuff on the labels. Michael Titford USA Pathology Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Wed Jun 23 08:34:44 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Wed Jun 23 08:35:18 2010 Subject: [Histonet] Prefilled Formalin Jars Message-ID: Hi histonetters: Was wondering if anybody knew of a vendor that had prefilled formalin jars that have a seal on them? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From Bauer.Karen <@t> mayo.edu Wed Jun 23 08:42:30 2010 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Wed Jun 23 08:42:36 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <53FC421CC200C5429929EDE6C3676F30F0A603@msgebe34> Rhonda, We keep our empty biopsy containers for one week and then dispose of them in a red bag. There are no "recommendations" that I know of, but we started doing it because there was a mix up with a biopsy case and we couldn't figure out if the nurse had put the specimens in the wrong labeled containers or if we labeled the containers wrong. If we had the containers to match it with, then we would have known where the mistake happened. It's helped us quite a few times since then. We find that most of the problems occur by nurses pre-labeling the containers and then putting the specimen in the wrong labeled container. Mostly GI biopsies, so the docs can tell the difference when looking through the microscope and then tell us that specimens were switched. When we look through our empty containers, we can see that we labeled everything up correctly and gross descriptions match the tissue slides, so we did everything correctly and the specimens must have been put in the wrong containers. Errors are usually caught the next day, so we probably don't have to save containers for a week. It's just easier to remember when we write a dump day tag on the bucket we store them in. Hope this helps, Karen Karen L. Bauer HT/HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System bauer.karen@mayo.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hartz, Rhonda SktnHR Sent: Tuesday, June 22, 2010 4:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> cvmc.org Wed Jun 23 08:55:45 2010 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Wed Jun 23 08:55:53 2010 Subject: [Histonet] Retaining empty containers In-Reply-To: Message-ID: We keep all of our empty containers for one week in plastic biohazard buckets and we label them Monday, Tuesday, etc. (same as Rosa). Saving them has saved our butt more than a few times! Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From cparsons <@t> vt.edu Wed Jun 23 10:16:02 2010 From: cparsons <@t> vt.edu (Parsons, Catherine) Date: Wed Jun 23 10:16:08 2010 Subject: [Histonet] Help Resurrecting Dried Fixed Tissue Message-ID: I am grasping at straws and hoping for ideas to salvage some formalin fixed tissue specimens. I work in a research lab, and am quite new to histology and this particular lab. A former student left formalin fixed tissue specimens that were stored in 70% ethanol. Unfortunately, the seal failed on a few of the vials, the ethanol evaporated, and the tissue is a dried out disaster at the bottom of the vial. Has anyone has any experience / luck rehydrating tissues? Really, any information that I could possibly pull out of these specimens would be welcome. Cathy Parsons From integrated.histo <@t> gmail.com Wed Jun 23 10:16:39 2010 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Wed Jun 23 10:16:44 2010 Subject: [Histonet] Breezy lab Message-ID: We solved the air vent problem by first taping a file folder to the overhead vent so it directed the flow away from our cutting stations. That worked somewhat, but not completely. So we then taped a file folder to the inside of the grate over the vent and now - no more breeze! But i can't wait to see what the maintenance people will say when they come and clean the vents - a once a year event. Cindy Hi Josie and Chris, I've worked in labs with similar problems. Breezes are nice outdoors but cause havoc with ribbons. If you can't get them to put in a door (which you'll probably have to lock since folks will walk in, shut the door and cause a breeze that way) then how about those fuzzy cubicle walls? The ones that are used in prairie dog farms (I mean open office situations ;-) ). Also, you'll need to install some type of baffle or other item that hangs from the air ducts and deflects the air across the ceiling instead of straight down. Good luck! Paula :-) From talulahgosh <@t> gmail.com Wed Jun 23 10:35:51 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Jun 23 10:35:56 2010 Subject: [Histonet] pancreatin Message-ID: Hello histonetters! I'm trying to make a pancreatin solution from Sigma pancreatin. There is no information with the actual product however, and the protocols we're following are vague about what to dissolve it in. They say to use a "balanced salt solution without Ca 2+ and Mg 2+". We assumed this is PBS but the pancreatin (or the salts in the PBS) precipitated out when I added it. Does anyone have any idea what we could use? We are using it for cleaning extra tissue off of chick or quail neural tube transplants, if that helps. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose From MSHERWOOD <@t> PARTNERS.ORG Wed Jun 23 10:42:03 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jun 23 10:42:13 2010 Subject: [Histonet] Retaining empty containers In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E243F7@PHSXMB30.partners.org> We too save our containers; on several occasions we had the investigator question the coding (usually their fault)! We keep ours for @a month (overkill), and then empty the formalin into waste container for pickup and discard the bottles. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Wednesday, June 23, 2010 9:56 AM To: 'Hartz, Rhonda SktnHR'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retaining empty containers We keep all of our empty containers for one week in plastic biohazard buckets and we label them Monday, Tuesday, etc. (same as Rosa). Saving them has saved our butt more than a few times! Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Goodwd2 <@t> LabCorp.com Wed Jun 23 10:47:39 2010 From: Goodwd2 <@t> LabCorp.com (Goodwin, Diana) Date: Wed Jun 23 10:49:13 2010 Subject: [Histonet] RE:Empty specimen containers Message-ID: We retain our empty containers for 2 weeks post-final report. D. Goodwin SJGI Marlton, NJ ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Wednesday, June 23, 2010 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Breezy lab/flyaway ribbons! (R J VAZQUEZ) 2. slim jims (Perry, Margaret) 3. RE: Breezy lab/flyaway ribbons! (Gill, Caula A.) 4. Re: Autofluorescence in murine white adipose tissue cryosections (Johnson, Teri) 5. Re: slim jims (V. Neubert) 6. X-gal staining troubleshooting (Johnson, Teri) 7. Glut1 antibody (Martha Ward) 8. RE: Breezy lab/flyaway ribbons! (Sebree Linda A) 9. Re: slim jims (Emily Sours) 10. IHC for Legionella? (Harrison, Sandra C.) 11. (no subject) (Hartz, Rhonda SktnHR) 12. Re: IHC for Legionella? (Richard Cartun) 13. Re: Help! In need of positive Gram Control (Jay Lundgren) 14. Re: (no subject) (Jennifer MacDonald) 15. Oven for paraffin slide drying (Mia Woodruff) 16. Re: Help! In need of positive Gram Control (Jackie M O'Connor) (Robert Richmond) 17. Re: Oven for paraffin slide drying (Emily Sours) 18. Re: Re: Amyloidosis control tissue (Komal Gada) 19. RE: Help! In need of positive Gram Control (connie grubaugh) 20. RE: New CAP question ANP.22760 (JMyers1@aol.com) 21. RE: Help! In need of positive Gram Control (Susan.Walzer@HCAHealthcare.com) 22. RE: Glut1 antibody (Hoekert, W.E.J.) 23. Retaining empty jars (mtitford@aol.com) ---------------------------------------------------------------------- Message: 1 Date: Tue, 22 Jun 2010 11:07:32 -0700 From: R J VAZQUEZ Subject: RE: [Histonet] Breezy lab/flyaway ribbons! To: , , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways. Robyn Vazquez > From: histotech@imagesbyhopper.com > To: trathborne@somerset-healthcare.com; JCBRITTON@Cheshire-Med.COM; histonet@lists.utsouthwestern.edu > Date: Tue, 22 Jun 2010 11:10:29 -0400 > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > CC: > > We have a similar situation, but I have shown my techs how to 1) wet the > brush they use to take the ribbon off the knife and 2) to roll/curl the > ribbon over the damp end of the brush and 3) hover our hand over the top of > the ribbon while moving it (to reduce the breeze) from the microtome to the > waterbath. > > I learned this technique when I first started in Histology, as we had a > choice, a HOT room with no AC and no wind, or learn how to deal with the > breeze and don't sweat! I opted for dealing with the wind. Now, it's > second nature and it matters not whether there is wind or not, I still use > the same motion for tranfer of the ribbon! > > Good luck with your situation. :o) > > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, > Toni > Sent: Tuesday, June 22, 2010 8:50 AM > To: Josie Britton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > > > For the ceiling vents, you could ask to have deflectors installed. The ones > we have are made of stainless, are slightly larger than the vent, and are > suspended about 6"-8" below the opening. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Josie Britton > Sent: Tuesday, June 22, 2010 6:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breezy lab/flyaway ribbons! > > > We have been having trouble with big breeze blowing our precious ribbons > > out of our hands while cutting. We would like a door on the Histology > > lab to cut down on the breeze of people walking by through the hall. > > Our facilities want to find out what other people are doing to stop this > > problem. We also have air ducts blowing down from above, which is not > > helping the problem. We would like as many labs solutions as possible. > > Our facilities have come up with all these crazy barriers that we would > > have to move to walk around when we need to put our racks on the > > stainer, answer timers, print more slides, use the oven, etc... > > > > > > > > Any input would be appreciated! > > > > > > > > Breezy girls, > > > > > > > > Josie Britton and Chris Braaten > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any attachments, > is for the sole use of the intended recipients and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by electronic mail and destroy all copies of the original > message. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error by > e-mail or you may call Somerset Medical Center's computer Help Desk at > 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: 06/22/10 > 06:36:00 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 22 Jun 2010 13:47:33 -0500 From: "Perry, Margaret" Subject: [Histonet] slim jims To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Do you put the slim jims in formalin and then process them or just put them in the processor? Margaret ------------------------------ Message: 3 Date: Tue, 22 Jun 2010 15:41:41 -0400 From: "Gill, Caula A." Subject: RE: [Histonet] Breezy lab/flyaway ribbons! To: "R J VAZQUEZ" , , , , Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9EAD@MDGEN-EXCH1.marylandgeneral.org> Content-Type: text/plain; charset="us-ascii" Ditto, I use a small brush that I got from the craft store and prior to picking up the ribbon I wet the brush and slip it under my ribbon end closest to the blade and presto no flyaways. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R J VAZQUEZ Sent: Tuesday, June 22, 2010 2:08 PM To: histotech@imagesbyhopper.com; trathborne@somerset-healthcare.com; jcbritton@cheshire-med.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways. Robyn Vazquez > From: histotech@imagesbyhopper.com > To: trathborne@somerset-healthcare.com; JCBRITTON@Cheshire-Med.COM; > histonet@lists.utsouthwestern.edu > Date: Tue, 22 Jun 2010 11:10:29 -0400 > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > CC: > > We have a similar situation, but I have shown my techs how to 1) wet > the brush they use to take the ribbon off the knife and 2) to > roll/curl the ribbon over the damp end of the brush and 3) hover our > hand over the top of the ribbon while moving it (to reduce the breeze) > from the microtome to the waterbath. > > I learned this technique when I first started in Histology, as we had > a choice, a HOT room with no AC and no wind, or learn how to deal with > the breeze and don't sweat! I opted for dealing with the wind. Now, > it's second nature and it matters not whether there is wind or not, I > still use the same motion for tranfer of the ribbon! > > Good luck with your situation. :o) > > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Rathborne, Toni > Sent: Tuesday, June 22, 2010 8:50 AM > To: Josie Britton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > > > For the ceiling vents, you could ask to have deflectors installed. The > ones we have are made of stainless, are slightly larger than the vent, > and are suspended about 6"-8" below the opening. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breezy lab/flyaway ribbons! > > > We have been having trouble with big breeze blowing our precious > ribbons > > out of our hands while cutting. We would like a door on the Histology > > lab to cut down on the breeze of people walking by through the hall. > > Our facilities want to find out what other people are doing to stop > this > > problem. We also have air ducts blowing down from above, which is not > > helping the problem. We would like as many labs solutions as possible. > > Our facilities have come up with all these crazy barriers that we > would > > have to move to walk around when we need to put our racks on the > > stainer, answer timers, print more slides, use the oven, etc... > > > > > > > > Any input would be appreciated! > > > > > > > > Breezy girls, > > > > > > > > Josie Britton and Chris Braaten > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail > and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical > Center and are intended only for the addressee. The information > contained in this message is confidential and may contain privileged, > confidential, proprietary and/or trade secret information entitled to > protection and/or exemption from disclosure under applicable law. > Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you > are not the addressee, please promptly delete this message and notify > the sender of the delivery error by e-mail or you may call Somerset > Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: > 06/22/10 06:36:00 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 22 Jun 2010 14:52:37 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: Autofluorescence in murine white adipose tissue cryosections To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Frank, Good luck with ridding your samples of autofluorescence. Fat is always very brightly fluorescent and I suspect it might even be so without any aldehyde fixation. First I wondered how successful you'd be using Sudan Black B, knowing it is a fat stain, and knowing that it's been published for this purpose. So you might try just that and see how that works. In addition you could try using a combination of UV irradiation and the Sudan Black B and see if the combination works for you. They use paraffin sections in this reference, but it might work as well with cryosections. http://www.microscopyu.com/references/pdfs/Viegas_etal_Eur_J_Histochem-51-59-2007.pdf Good luck, and do report back if you find a solution to this dilemma! Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ------------------------------ Message: 5 Date: Tue, 22 Jun 2010 21:58:39 +0200 From: "V. Neubert" Subject: Re: [Histonet] slim jims To: histonet@lists.utsouthwestern.edu Message-ID: <4C2115EF.7030404@vneubert.com> Content-Type: text/plain; charset=ISO-8859-1 I just found "a partially eaten Slim Jim snack" picture on wikipedia... Is it Friday again? :-> > Do you put the slim jims in formalin and then process them or just put them in the processor? > Margaret > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 6 Date: Tue, 22 Jun 2010 15:02:44 -0500 From: "Johnson, Teri" Subject: [Histonet] X-gal staining troubleshooting To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, I have a question about whole mount x-gal staining. Do any of you have experience with your samples turning brown after incubation with the x-gal? If yes, do you know what causes this and how to keep it from happening? Thanks in advance, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ------------------------------ Message: 7 Date: Tue, 22 Jun 2010 16:10:12 -0400 From: "Martha Ward" Subject: [Histonet] Glut1 antibody To: Message-ID: <61135F0455D33347B5AAE209B903A30433DEBA4A@EXCHVS2.medctr.ad.wfubmc.edu> Content-Type: text/plain; charset="us-ascii" I have been using anti-human Glut-1 from Dako for several years but when I went to order it I was told it was discontinued. Does anyone have a suggestion of where I can locate this antibody? Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ------------------------------ Message: 8 Date: Tue, 22 Jun 2010 15:28:50 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] Breezy lab/flyaway ribbons! To: "Gill, Caula A." , "R J VAZQUEZ" , , , , Message-ID: <8C023B4AB999614BA4791BAEB26E2738399EDC@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" I do that too using either a wet brush or applicator stick but sometimes the breeze is more like a gale force wind breaking my ribbon apart to then adhere to everything but the surface of my waterbath! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gill, Caula A. Sent: Tuesday, June 22, 2010 2:42 PM To: R J VAZQUEZ; histotech@imagesbyhopper.com; trathborne@somerset-healthcare.com; jcbritton@cheshire-med.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! Ditto, I use a small brush that I got from the craft store and prior to picking up the ribbon I wet the brush and slip it under my ribbon end closest to the blade and presto no flyaways. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R J VAZQUEZ Sent: Tuesday, June 22, 2010 2:08 PM To: histotech@imagesbyhopper.com; trathborne@somerset-healthcare.com; jcbritton@cheshire-med.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways. Robyn Vazquez > From: histotech@imagesbyhopper.com > To: trathborne@somerset-healthcare.com; JCBRITTON@Cheshire-Med.COM; > histonet@lists.utsouthwestern.edu > Date: Tue, 22 Jun 2010 11:10:29 -0400 > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > CC: > > We have a similar situation, but I have shown my techs how to 1) wet > the brush they use to take the ribbon off the knife and 2) to > roll/curl the ribbon over the damp end of the brush and 3) hover our > hand over the top of the ribbon while moving it (to reduce the breeze) > from the microtome to the waterbath. > > I learned this technique when I first started in Histology, as we had > a choice, a HOT room with no AC and no wind, or learn how to deal with > the breeze and don't sweat! I opted for dealing with the wind. Now, > it's second nature and it matters not whether there is wind or not, I > still use the same motion for tranfer of the ribbon! > > Good luck with your situation. :o) > > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Rathborne, Toni > Sent: Tuesday, June 22, 2010 8:50 AM > To: Josie Britton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > > > For the ceiling vents, you could ask to have deflectors installed. The > ones we have are made of stainless, are slightly larger than the vent, > and are suspended about 6"-8" below the opening. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breezy lab/flyaway ribbons! > > > We have been having trouble with big breeze blowing our precious > ribbons > > out of our hands while cutting. We would like a door on the Histology > > lab to cut down on the breeze of people walking by through the hall. > > Our facilities want to find out what other people are doing to stop > this > > problem. We also have air ducts blowing down from above, which is not > > helping the problem. We would like as many labs solutions as possible. > > Our facilities have come up with all these crazy barriers that we > would > > have to move to walk around when we need to put our racks on the > > stainer, answer timers, print more slides, use the oven, etc... > > > > > > > > Any input would be appreciated! > > > > > > > > Breezy girls, > > > > > > > > Josie Britton and Chris Braaten > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail > and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical > Center and are intended only for the addressee. The information > contained in this message is confidential and may contain privileged, > confidential, proprietary and/or trade secret information entitled to > protection and/or exemption from disclosure under applicable law. > Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you > are not the addressee, please promptly delete this message and notify > the sender of the delivery error by e-mail or you may call Somerset > Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: > 06/22/10 06:36:00 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 22 Jun 2010 16:50:06 -0400 From: Emily Sours Subject: Re: [Histonet] slim jims To: "Perry, Margaret" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 if by "processor" you mean "my mouth", yes. the formalin is well, just a formality. HA! Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Tue, Jun 22, 2010 at 2:47 PM, Perry, Margaret wrote: > Do you put the slim jims in formalin and then process them or just put them > in the processor? > Margaret > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Tue, 22 Jun 2010 16:21:51 -0500 From: "Harrison, Sandra C." Subject: [Histonet] IHC for Legionella? To: Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone know of an IHC antibody for Legionella? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ------------------------------ Message: 11 Date: Tue, 22 Jun 2010 15:55:07 -0600 From: "Hartz, Rhonda SktnHR" Subject: [Histonet] (no subject) To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca ------------------------------ Message: 12 Date: Tue, 22 Jun 2010 18:31:49 -0400 From: "Richard Cartun" Subject: Re: [Histonet] IHC for Legionella? To: , "Sandra C. Harrison" Message-ID: <4C210194.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII I offer IHC for Legionella in my laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Harrison, Sandra C." 6/22/2010 5:21 PM >>> Does anyone know of an IHC antibody for Legionella? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 22 Jun 2010 17:52:34 -0500 From: Jay Lundgren Subject: Re: [Histonet] Help! In need of positive Gram Control To: DianaRip1@aol.com Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Slim Jim works great. This is what we used to use at AFIP. Just slice it and process it the same as your regular tissue. Jay A. Lundgren, M.S., HTL (ASCP) Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 22 Jun 2010 15:57:21 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] (no subject) To: "Hartz, Rhonda SktnHR" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We used to keep them for a week in a bag. If there were no problems at the end of the week we disposed of them "Hartz, Rhonda SktnHR" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/22/2010 03:24 PM To cc Subject [Histonet] (no subject) Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 23 Jun 2010 09:06:14 +1000 From: Mia Woodruff Subject: [Histonet] Oven for paraffin slide drying To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all, I need to purchase an oven for drying slides, on a very limited budget. I came across a "food dehydrator" which looks like an oven and has drawers which are the perfect size for the slide holders to fit into and it can reach a temperature of 60C - and it costs about 1/8 of the price of a normal oven. Can anyone foresee any issues in me using this for drying paraffin slides? As far as I can tell there is a low air flow inside and the temp can be set up to 60 degrees so it seems perfect? Any comments? Thanks Mia ------------------------------ Message: 16 Date: Tue, 22 Jun 2010 19:34:09 -0400 From: Robert Richmond Subject: [Histonet] Re: Help! In need of positive Gram Control (Jackie M O'Connor) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I agree - acute appendicitis with rupture and an abscess provides an abundance of gram positive and gram negative bacteria, and such cases are common. I think that actual clinical tissue is better than controls faked with reference organisms. Tissue gram stains are greatly overrated. They don't work nearly as well as smears do. If you want to see bacteria in tissue sections, stain them with Giemsa or toluidine blue. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 17 Date: Tue, 22 Jun 2010 20:16:41 -0400 From: Emily Sours Subject: Re: [Histonet] Oven for paraffin slide drying To: Mia Woodruff , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Do slide warmers cost too much? I don't know how much they cost, though, maybe an oven is cheaper. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Tue, Jun 22, 2010 at 7:06 PM, Mia Woodruff wrote: > Hello all, > > I need to purchase an oven for drying slides, on a very limited budget. I came across a "food dehydrator" which looks like an oven and has drawers which are the perfect size for the slide holders to fit into and it can reach a temperature of 60C - and it costs about 1/8 of the price of a normal oven. > Can anyone foresee any issues in me using this for drying paraffin slides? As far as I can tell there is a low air flow inside and the temp can be set up to 60 degrees so it seems perfect? Any comments? > > Thanks > Mia > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 18 Date: Tue, 22 Jun 2010 21:01:14 -0400 From: Komal Gada Subject: Re: [Histonet] Re: Amyloidosis control tissue To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 American mastertech On Tue, Jun 22, 2010 at 12:19 PM, Robert Richmond wrote: > It's always been difficult to get amyloid control tissue. Amyloidosis > is a rather rare disease in the USA, and some cases simply do not > stain well. > > I've asked this question on Histonet several times and no one has ever > responded: Amyloidosis (of the AA type I think) is rather easily > produced in mice and other small experimental animals. Why can't this > tissue be available as an amyloid control? > > Seems that some enterprising person could make a little money this way. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 19 Date: Tue, 22 Jun 2010 18:06:14 -0700 From: connie grubaugh Subject: RE: [Histonet] Help! In need of positive Gram Control To: , , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Tried the slim jim and all of my doctors did not like it. Don't waste your time. Connie G. > Date: Tue, 22 Jun 2010 10:16:14 -0400 > From: cgill@marylandgeneral.org > To: JCBRITTON@Cheshire-Med.COM; DianaRip1@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > CC: > > You have got to be kidding!! That's hysterical. So process a slim jim > and you have > Gram - and + controls. If you're serious I'm trying it. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:10 AM > To: DianaRip1@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > > > > > > Have you tried a Slim Jim? They have gram positive and negative rods in > > them. Regardless, I still enjoy eating them once and a while! > > > > > > > > Josie Britton Ht > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > DianaRip1@aol.com > > Sent: Monday, June 21, 2010 7:43 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Help! In need of positive Gram Control > > > > > > > > Help! We are in need of positive Gram Control Blocks if anyone has any > > > > extra they are willing to part with. I have lots of Fungus, > > Pneumocystis and > > > > HPV tissue blocks to trade. > > > > > > > > Diana Ripley > > > > John Muir Histology > > > > Concord Campus > > > > 2540 East Street > > > > Concord, CA 94520 > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 ------------------------------ Message: 20 Date: Tue, 22 Jun 2010 21:50:42 EDT From: JMyers1@aol.com Subject: RE: [Histonet] New CAP question ANP.22760 To: tjasper@copc.net Cc: histonet@lists.utsouthwestern.edu Message-ID: <7cdd9.6a7042fa.3952c272@aol.com> Content-Type: text/plain; charset="US-ASCII" Tom: As much as I agree with your acknowledgment that its seems a bit odd for the CAP to have a blood-banker responding to AP-related issue, I'm actually not surprised. The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time, and they wonder why IHC isn't expected to follow the same requirements as chemistry, immunology, etc. -- IHC is, after all, an awful lot like ELISA. And rightfully so, because IHC is, under CLIA (which supersedes CAP), considered highly-complex, non-waived testing -- and is, therefore, subject to the same Quality Systems regulations (in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. Could it be that, because AP produces qualitative results that are interpreted by a pathologist and CP produces quantitative results that are interpreted by an analyzer, we somehow think that CLIA rules don't apply to IHC? I certainly don't have the answer to that, but it make me wonder what the future holds. As witnessed by some of the newest CAP 'standards' (including the question in question...no pun intended), e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens must be tested, and where 10 of the positives must be weakly positive -- an acknowledgment that validation specimens must be carefully selected in order to obtain appropriate results), it certainly doesn't appear that the regulation of IHC testing is going to become more relaxed. Joe Myers, M.S., CT(ASCP) ------------------------------ Message: 12 Date: Fri, 18 Jun 2010 12:38:07 -0700 From: "Thomas Jasper" Subject: RE: [Histonet] New CAP question ANP.22760 To: "Mark Tarango" Cc: _histonet@lists.utsouthwestern.edu_ (mailto:histonet@lists.utsouthwestern.edu) Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 ------------------------------ Message: 21 Date: Wed, 23 Jun 2010 02:14:47 -0500 From: Subject: RE: [Histonet] Help! In need of positive Gram Control To: , , , , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AF9784613@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Take some fresh tissue (we use umbilical cord) to micro and let them incubate in for a few days in gram + and/or negative. Then fix it and you will have good controls. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: Tuesday, June 22, 2010 9:06 PM To: cgill@marylandgeneral.org; jcbritton@cheshire-med.com; dianarip1@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Tried the slim jim and all of my doctors did not like it. Don't waste your time. Connie G. > Date: Tue, 22 Jun 2010 10:16:14 -0400 > From: cgill@marylandgeneral.org > To: JCBRITTON@Cheshire-Med.COM; DianaRip1@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > CC: > > You have got to be kidding!! That's hysterical. So process a slim jim > and you have > Gram - and + controls. If you're serious I'm trying it. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:10 AM > To: DianaRip1@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > > > > > > Have you tried a Slim Jim? They have gram positive and negative rods in > > them. Regardless, I still enjoy eating them once and a while! > > > > > > > > Josie Britton Ht > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > DianaRip1@aol.com > > Sent: Monday, June 21, 2010 7:43 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Help! In need of positive Gram Control > > > > > > > > Help! We are in need of positive Gram Control Blocks if anyone has any > > > > extra they are willing to part with. I have lots of Fungus, > > Pneumocystis and > > > > HPV tissue blocks to trade. > > > > > > > > Diana Ripley > > > > John Muir Histology > > > > Concord Campus > > > > 2540 East Street > > > > Concord, CA 94520 > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Wed, 23 Jun 2010 10:24:22 +0200 From: "Hoekert, W.E.J." Subject: RE: [Histonet] Glut1 antibody To: "Martha Ward" , Message-ID: <1190CB05C44B13409483514729C2FC360C0ADF@PAIT42.olvg.nl> Content-Type: text/plain; charset="iso-8859-1" We switched to Neomarkers (polyclonal) (RB 078-A). Willem Hoekert OLVG, Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Martha Ward Verzonden: di 22-6-2010 22:10 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Glut1 antibody I have been using anti-human Glut-1 from Dako for several years but when I went to order it I was told it was discontinued. Does anyone have a suggestion of where I can locate this antibody? Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ------------------------------ Message: 23 Date: Wed, 23 Jun 2010 08:25:33 -0400 From: mtitford@aol.com Subject: [Histonet] Retaining empty jars To: Histonet@pathology.swmed.edu Message-ID: <8CCE0E64F6A9C4A-FCC-6F33@Webmail-d119.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Rhonda Hartz asks if anyone else retains empty jars after grossing. We too retain our empty jars after grossing for about a month. We keep them in a red biohazard trash bag. It pays dividends. If once the case has been signed out the physician/surgeon complains that they removed more biopsies than we listed in the report, or submitted more jars, etc, we can go back and check. On rare occasions we have found tiny pieces of tissue in the jars, or hidden in the grooves of the lid. These enquiries most often happen with tiny pieces of tissue, of course, like GI biopsies. We have the physicians write on the label on the jar what is inside it (like everyone else I guess) as well as listing it on the surgical pathology slip. You can also go back and check what they wrote down. Afterwards the jars are incinerated as they have all that HIPPA related stuff on the labels. Michael Titford USA Pathology Mobile AL USA ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 28 **************************************** ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA. From BSullivan <@t> shorememorial.org Wed Jun 23 10:48:01 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Wed Jun 23 10:50:19 2010 Subject: [Histonet] Prefilled Formalin Jars In-Reply-To: Message-ID: To my recollection, Surgipath Medical had sealed pre-filled containers. It has been some time since I ordered from them so this might not be the case now. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Sara Baldwin/mhhcc.org " To rg> cc Sent by: histonet-bounces@ Subject lists.utsouthwest [Histonet] Prefilled Formalin Jars ern.edu 06/23/2010 09:34 AM Hi histonetters: Was wondering if anybody knew of a vendor that had prefilled formalin jars that have a seal on them? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Wed Jun 23 11:04:06 2010 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jun 23 11:04:26 2010 Subject: [Histonet] Retaining empty jars In-Reply-To: <07732CE52EC3174AB891DE1C62DB4D8FC7B81F@GIEXCHANGE.gidocs.net> Message-ID: CAP retention guidelines say that wet tissues are to be retained for 2 weeks after final signout. We keep all containers, empty or not, for the 2 weeks then strain and discard them in biohazard trash. We have a large cabinet and designate a shelf for each day. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Wednesday, June 23, 2010 9:09 AM To: mtitford@aol.com; Histonet@pathology.swmed.edu Subject: RE: [Histonet] Retaining empty jars We also retain ours; for about 2 weeks. I have plastic bins labeled Monday 1/ Monday 2.. ect.. that way I don't have to search through all of them if I have a question about a case. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Wednesday, June 23, 2010 7:26 AM To: Histonet@pathology.swmed.edu Subject: [Histonet] Retaining empty jars Rhonda Hartz asks if anyone else retains empty jars after grossing. We too retain our empty jars after grossing for about a month. We keep them in a red biohazard trash bag. It pays dividends. If once the case has been signed out the physician/surgeon complains that they removed more biopsies than we listed in the report, or submitted more jars, etc, we can go back and check. On rare occasions we have found tiny pieces of tissue in the jars, or hidden in the grooves of the lid. These enquiries most often happen with tiny pieces of tissue, of course, like GI biopsies. We have the physicians write on the label on the jar what is inside it (like everyone else I guess) as well as listing it on the surgical pathology slip. You can also go back and check what they wrote down. Afterwards the jars are incinerated as they have all that HIPPA related stuff on the labels. Michael Titford USA Pathology Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From histotech <@t> imagesbyhopper.com Wed Jun 23 11:34:20 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Jun 23 11:34:35 2010 Subject: [Histonet] Prefilled Formalin Jars In-Reply-To: Message-ID: <84DF48587497459B9C489D95ABF92ABD@hopperPC> Richard Allen (now available via Fisher Sci) carries pre-filled bottles. I get the 15ml, 30ml, 60ml, 120ml and 180ml. Oh wait, I just read more clearly, these don't have "seals" on them, just a screw-top lid. Not sure that helps you. :O( Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Wednesday, June 23, 2010 11:48 AM To: Sara Baldwin/mhhcc.org Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Prefilled Formalin Jars To my recollection, Surgipath Medical had sealed pre-filled containers. It has been some time since I ordered from them so this might not be the case now. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Sara Baldwin/mhhcc.org " To rg> cc Sent by: histonet-bounces@ Subject lists.utsouthwest [Histonet] Prefilled Formalin Jars ern.edu 06/23/2010 09:34 AM Hi histonetters: Was wondering if anybody knew of a vendor that had prefilled formalin jars that have a seal on them? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: 06/22/10 06:36:00 From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Jun 23 11:32:49 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Jun 23 11:34:37 2010 Subject: [Histonet] RE: Help Resurrecting Dried Fixed Tissue In-Reply-To: References: Message-ID: I once took tissue that had dried up after being fixed in formalin and soaked it in normal saline to rehydrate it. Very small piece of skin that I soaked for about 3 hours. Then placed the tissue back into formalin and reprocessed routinely. It did not have the best morphology but is was usable. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parsons, Catherine [cparsons@vt.edu] Sent: Wednesday, June 23, 2010 11:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help Resurrecting Dried Fixed Tissue I am grasping at straws and hoping for ideas to salvage some formalin fixed tissue specimens. I work in a research lab, and am quite new to histology and this particular lab. A former student left formalin fixed tissue specimens that were stored in 70% ethanol. Unfortunately, the seal failed on a few of the vials, the ethanol evaporated, and the tissue is a dried out disaster at the bottom of the vial. Has anyone has any experience / luck rehydrating tissues? Really, any information that I could possibly pull out of these specimens would be welcome. Cathy Parsons _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Jun 23 11:48:48 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Jun 23 11:49:01 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: <7cdd9.6a7042fa.3952c272@aol.com> References: <7cdd9.6a7042fa.3952c272@aol.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C021B89F2FB@EXMBMCB15.ucsfmedicalcenter.org> Joe, You wrote : The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time ..." Those were my thoughts exactly. While the person replying may or may not have specific histology experience she will have clinical lab experience (however, my guess is that she is exposed to histology regularly at CAP). Clinical labs have a bit of an easier time, actually, because they validate primarily to known concentration controls - analytical controls manufactured at a range of known concentrations for instance. The institution then adds in their normal controls for validation. As far as the current question about validating a new lot of reagent the best practice is to run parallel tests on the same machine. If that is not easily possible on a particular manufacturer's instrument then the question should be asked of them: Why not? If this is a requirement the manufacturer should provide an easy path to meeting the requirement. However, if that is not the case then the institution simply writes a procedure to get around the inadequacies of the instrument (Maybe the vendor can help with that). Then follow the procedure. That should satisfy inspectors. An "...appropriate panel of tissues..." is whatever the institution deems appropriate for the given antibody or reagent. This is a perfect place for tissue arrays. You can make your own or buy them. IHC must meet CLIA validation guidelines but since IHC is generally qualitative the requirements must be understood and methods adapted to a qualitative scenario. Several IHC and Histotechnology books discuss the subject at length (Taylor, Dabbs, Bancroft for instance). Below is a brief overview of how to do that. (for more in-depth info this was covered in an NSH teleconference I gave last year - PowerPoint, audio and references available from NSH-, and will be covered in a similar workshop at NSH in Seattle this year). 1) CAP General Validation CAP GEN.42020-42163 Test Method Validation Follows CLIA CFR Sec 493.1253 Does not apply well to IHC (IHC is usually qualitative) But the general principle applies: The laboratory must have data on each test's accuracy, precision, analytic sensitivity, interferences and reportable range. Unmodified FDA-cleared or approved tests: the lab may use manufacturer information or published reports but lab must verify outside data. Non-FDA cleared: Lab MUST verify or establish analytic accuracy, precision, sensitivity, specificity and reportable range. 2) Validation includes: Accuracy: Compare results with New antibody to a previously validated antibody on the same tissues Precision: Test samples with varying antigen expression Intra-run, Inter-run tests, 10 slides each (reproducibility) Sensitivity: True Positive vs False Negative (higher % FN = less sensitive) Interferences [Specificity]: True Negative vs False Positive (Higher % FP = less specific) Delineate what could interfere to give a false positive or false negative result. Reportable Range Establish a scoring system Provide the definition of a positive result 3)Sensitivity Analytic Sensitivity: Lowest amount of substance detectable by the test Can only be done with controls of known concentration Diagnostic Sensitivity: Ability of the test to determine true diagnostic positive verses false negative (higher % FN = less sensitive) Requires comparison to a previously validated antibody IHC Sensitivity: Extent to which an antibody can be diluted and still achieve target recognition. NOTE: This is determined by antibody AND detection system! 4) Specificity: Analytic Specificity Accuracy on tests of known positive and negative controls Controls of known concentration Determine what could "Interfere" to confound the result Diagnostic Specificity Ability of a test to determine true diagnostic negative verses false positives (Higher % FP = less specific) Requires comparison to a previously validated antibody IHC Specificity Ability of an antibody to bind exclusively to its particular antigen in the absence of staining of other molecules Or, staining of other structures in addition to target structures/cells (Sensitivity and Specificity adapted from: Theoretical and Practical Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote, 2005) Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMyers1@aol.com Sent: Tuesday, June 22, 2010 6:51 PM To: tjasper@copc.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Tom: As much as I agree with your acknowledgment that its seems a bit odd for the CAP to have a blood-banker responding to AP-related issue, I'm actually not surprised. The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time, and they wonder why IHC isn't expected to follow the same requirements as chemistry, immunology, etc. -- IHC is, after all, an awful lot like ELISA. And rightfully so, because IHC is, under CLIA (which supersedes CAP), considered highly-complex, non-waived testing -- and is, therefore, subject to the same Quality Systems regulations (in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. Could it be that, because AP produces qualitative results that are interpreted by a pathologist and CP produces quantitative results that are interpreted by an analyzer, we somehow think that CLIA rules don't apply to IHC? I certainly don't have the answer to that, but it make me wonder what the future holds. As witnessed by some of the newest CAP 'standards' (including the question in question...no pun intended), e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens must be tested, and where 10 of the positives must be weakly positive -- an acknowledgment that validation specimens must be carefully selected in order to obtain appropriate results), it certainly doesn't appear that the regulation of IHC testing is going to become more relaxed. Joe Myers, M.S., CT(ASCP) ------------------------------ Message: 12 Date: Fri, 18 Jun 2010 12:38:07 -0700 From: "Thomas Jasper" Subject: RE: [Histonet] New CAP question ANP.22760 To: "Mark Tarango" Cc: _histonet@lists.utsouthwestern.edu_ (mailto:histonet@lists.utsouthwestern.edu) Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Jun 23 11:58:09 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Jun 23 11:59:07 2010 Subject: [Histonet] Retaining empty jars References: Message-ID: Ditto. Claire re: CAP retention guidelines say that wet tissues are to be retained for 2 weeks after final signout. We keep all containers, empty or not, for the 2 weeks then strain and discard them in biohazard trash. From wdesalvo.cac <@t> hotmail.com Wed Jun 23 12:03:03 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Jun 23 12:03:13 2010 Subject: [Histonet] Retaining empty containers In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E243F7@PHSXMB30.partners.org> References: , , <073AE2BEA1C2BA4A8837AB6C4B943D9703E243F7@PHSXMB30.partners.org> Message-ID: We also keep the empty containers. The empty containers are kept one week, placed in hazardous bags and labeled by grosser/date, the formalin is emptied, at time of grossing, into our Formalin refiltering system (Creative Waste Solutions) and the recovered formalin is used at the grossing bench to fix cassettes waiting for processing. This reduces the waste formalin needed to be hauled out of the lab. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 23 Jun 2010 11:42:03 -0400 > From: MSHERWOOD@PARTNERS.ORG > To: Lynne.Bell@cvmc.org; Rhonda.Hartz@saskatoonhealthregion.ca; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Retaining empty containers > CC: > > We too save our containers; on several occasions we had the investigator > question the coding (usually their fault)! We keep ours for @a month > (overkill), and then empty the formalin into waste container for pickup and > discard the bottles. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne > Sent: Wednesday, June 23, 2010 9:56 AM > To: 'Hartz, Rhonda SktnHR'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Retaining empty containers > > We keep all of our empty containers for one week in plastic biohazard buckets > and we label them Monday, Tuesday, etc. (same as Rosa). Saving them has saved > our butt more than a few times! > > Lynne A. Bell, HT (ASCP) > Technical Specialist, Histology > Central Vermont Medical Center > 130 Fisher Road > Barre, VT 05641 > 802-371-4923 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From wdesalvo.cac <@t> hotmail.com Wed Jun 23 12:04:55 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Jun 23 12:07:21 2010 Subject: [Histonet] Prefilled Formalin Jars In-Reply-To: References: Message-ID: I am wondering why the need for a seal? William DeSalvo, B.S., HTL(ASCP) > From: sbaldwin@mhhcc.org > To: histonet@lists.utsouthwestern.edu > Date: Wed, 23 Jun 2010 09:34:44 -0400 > Subject: [Histonet] Prefilled Formalin Jars > > Hi histonetters: > Was wondering if anybody knew of a vendor that had prefilled formalin jars that have a seal on them? > > Thanks > Pathology Supervisor > Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center > sbaldwin@mhhcc.org > Ph 812-482-0210, 482-0216, Fax 812-482-0232, > Pager 812-481-0897 > Confidential information, Authorized use only. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4 From JCBRITTON <@t> Cheshire-Med.COM Wed Jun 23 12:45:31 2010 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Wed Jun 23 12:45:43 2010 Subject: [Histonet] slim jims In-Reply-To: References: Message-ID: We put them in the formalin first just like a specimen. Josie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Tuesday, June 22, 2010 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slim jims Do you put the slim jims in formalin and then process them or just put them in the processor? Margaret _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From JCBRITTON <@t> Cheshire-Med.COM Wed Jun 23 12:52:34 2010 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Wed Jun 23 12:52:47 2010 Subject: [Histonet] Help! In need of positive Gram Control In-Reply-To: <087A9911BBAFDE4B8151CB148586E2C23A9EAC@MDGEN-EXCH1.marylandgeneral.org> References: <26338.611bde74.39515318@aol.com> <087A9911BBAFDE4B8151CB148586E2C23A9EAC@MDGEN-EXCH1.marylandgeneral.org> Message-ID: It's true! Who knew that Gram + and- riddles meat sticks could be so yummy and versatile too! Josie -----Original Message----- From: Gill, Caula A. [mailto:cgill@marylandgeneral.org] Sent: Tuesday, June 22, 2010 10:16 AM To: Josie Britton; DianaRip1@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control You have got to be kidding!! That's hysterical. So process a slim jim and you have Gram - and + controls. If you're serious I'm trying it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Josie Britton Sent: Tuesday, June 22, 2010 6:10 AM To: DianaRip1@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Have you tried a Slim Jim? They have gram positive and negative rods in them. Regardless, I still enjoy eating them once and a while! Josie Britton Ht Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DianaRip1@aol.com Sent: Monday, June 21, 2010 7:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help! In need of positive Gram Control Help! We are in need of positive Gram Control Blocks if anyone has any extra they are willing to part with. I have lots of Fungus, Pneumocystis and HPV tissue blocks to trade. Diana Ripley John Muir Histology Concord Campus 2540 East Street Concord, CA 94520 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From histotech <@t> imagesbyhopper.com Wed Jun 23 13:02:52 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Jun 23 13:02:58 2010 Subject: [Histonet] Retaining empty jars In-Reply-To: Message-ID: <9200F478971A4C0384E7CA873DB42EEF@hopperPC> We keep our "empty" containers for 6 weeks after grossing, which is typically 5 weeks after signout. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, June 23, 2010 12:58 PM To: Histonet@pathology.swmed.edu Subject: RE: [Histonet] Retaining empty jars Ditto. Claire re: CAP retention guidelines say that wet tissues are to be retained for 2 weeks after final signout. We keep all containers, empty or not, for the 2 weeks then strain and discard them in biohazard trash. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: 06/22/10 06:36:00 From MadaryJ <@t> MedImmune.com Wed Jun 23 13:12:34 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Jun 23 13:12:47 2010 Subject: [Histonet] rehydrating dried out tissue and gram positive control(forget the slim jim) Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1301EE0295@MD1EV002.medimmune.com> I worked at AFIP for 16 years and worked in infectious disease for 6 and we never used a slim jim. People discussed it but the truth is there was literally thousands of gram positive samples to use, why would we have wanted to use a slim jim? Use appendix that has been tested. Dried out tissue as I recall an overnight soak in some 15ml glycerin, 65 ML NBFformalin and 20 ml Absolute alcohol and then wash in NBF a few times before the processor. Might work. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From gmartin <@t> marshallmedical.org Wed Jun 23 13:33:01 2010 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Jun 23 13:33:18 2010 Subject: [Histonet] Pap sign out Message-ID: <6ED9D4252F278841A0593D3D788AF24C09394C03@mailsvr.MARSHMED.local> We have always had our Pathologist sign out all Pap results, and are now considering having out Cytotech sign out Pap's. Is there an agency we have to report o before we start this process. Also, how are others choosing the 10% for review by the Pathologist? Thanks Gary From cmmathis1 <@t> bellsouth.net Wed Jun 23 13:59:45 2010 From: cmmathis1 <@t> bellsouth.net (RICKY MATHIS) Date: Wed Jun 23 13:59:51 2010 Subject: [Histonet] Special stain for neutrophils Message-ID: <77323.22802.qm@web180603.mail.sp1.yahoo.com> Hello my Histonet friends, Is there any special stain that you know of to demonstrate neutrophils and/or macrophages in tissue sections? Best, Cathy Cathy M. MathisHistology Core Technician Wake Forest Institute?for Regenerative Medicine Wake Forest University Health Sciences Medical Center Blvd. Winston-Salem, NC?? 27157 ph:? 336-713-7285 fax: 336-713-7290 email:cmmathis@wfubmc.edu From suhyoung.jeong <@t> gmail.com Wed Jun 23 15:16:36 2010 From: suhyoung.jeong <@t> gmail.com (Suhyoung Jeong) Date: Wed Jun 23 15:16:40 2010 Subject: [Histonet] mouse BBB endothelial marker? Message-ID: Dear everyone, Does anybody know a good primary antibody for the mouse BBB endothelial staining? Preferably mouse or rat so I can do double with a rabbit antibody, but it is not a big problem. Please let me know if you know a good marker. Thank you in advance, Suh From sfeher <@t> CMC-NH.ORG Wed Jun 23 16:50:45 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Jun 23 16:50:50 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B754B2@exchange.cmc-nh.org> We retain them until the specimen is signed out, usually no more than 3 days. This has been helpful if the specimen container labeling is called into question either by the pathologist (because the cellular profile does not match what the specimen source indicates) or the clinician. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hartz, Rhonda SktnHR Sent: Tuesday, June 22, 2010 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Jun 23 17:07:40 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Jun 23 17:07:49 2010 Subject: [Histonet] IHC for Legionella? Message-ID: Hi, I am not a big fan of Santa Cruz antibodies, but they have a fairly big list of micro-organism antibodies in their catalog. Here is a link to a list of Legionella antibodies from their website. http://www.scbt.com/table-legionella_pneumophila.html You may find what you need there. I thought of them because they were one of very few that carry Treponemia antibodies I was looking for recently. I have not used the antibodies on that page so I'm not saying they work, just that they are there. Best of luck, Amos From AnthonyH <@t> chw.edu.au Wed Jun 23 18:12:57 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jun 23 18:13:12 2010 Subject: [Histonet] Help Resurrecting Dried Fixed Tissue In-Reply-To: Message-ID: We have success with Sandison Method, see below: Sandison Method Solutions: 95% ethanol 300ml 38% Formalin 10ml Sodium carbonate 10g Water 690ml Method: Leave tissues in solution overnight or until they become soft. Replace one third of fluid with 95% ethanol, repeat until tissue becomes firm. Reference: "The histological examination of Mummified Tissue" Stain Techn 30:277-283, 1955 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parsons, Catherine Sent: Thursday, 24 June 2010 1:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help Resurrecting Dried Fixed Tissue I am grasping at straws and hoping for ideas to salvage some formalin fixed tissue specimens. I work in a research lab, and am quite new to histology and this particular lab. A former student left formalin fixed tissue specimens that were stored in 70% ethanol. Unfortunately, the seal failed on a few of the vials, the ethanol evaporated, and the tissue is a dried out disaster at the bottom of the vial. Has anyone has any experience / luck rehydrating tissues? Really, any information that I could possibly pull out of these specimens would be welcome. Cathy Parsons _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From collette2 <@t> llnl.gov Wed Jun 23 20:11:33 2010 From: collette2 <@t> llnl.gov (Collette, Nicole M.) Date: Wed Jun 23 20:11:38 2010 Subject: [Histonet] RE: X-gal staining troubleshooting In-Reply-To: References: Message-ID: <3AD1C8AEFB40D7408467F3A333A8A6D1019A34E26C77@NSPEXMBX-B.the-lab.llnl.gov> Hi, Teri, I have seen it in some of my stains, but they were always in embryos of mice from a particular strain (that we did not generate and have incomplete construct information for). I always attributed it to a different LacZ allele, as I have stained litters of a different strain side-by-side with the same reagents on the same day and got the expected blue stain, but it may be something else... If you find out, I'd like to know, too. Sincerely, Nicole Collette LLNL/ UC Berkeley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Tuesday, June 22, 2010 1:03 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] X-gal staining troubleshooting Hi all, I have a question about whole mount x-gal staining. Do any of you have experience with your samples turning brown after incubation with the x-gal? If yes, do you know what causes this and how to keep it from happening? Thanks in advance, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://*lists.utsouthwestern.edu/mailman/listinfo/histonet From BenatM <@t> gosh.nhs.uk Thu Jun 24 07:28:34 2010 From: BenatM <@t> gosh.nhs.uk (Malika Benatti) Date: Thu Jun 24 07:29:08 2010 Subject: [Histonet] Glut1 antibody In-Reply-To: <201006231246.TKB44376@rlmh01.swi.contact.secure-ops.net> References: <201006231246.TKB44376@rlmh01.swi.contact.secure-ops.net> Message-ID: <4C235D81.4626.0038.0@gosh.nhs.uk> ** Proprietary ** ** Reply Requested When Convenient ** We use anti-glut-1 (rabbit polyclonal) Purchased from Millipore http://www.millipore.com/catalogue/item/07-1401 Hope this helps, Malika ------------------------------ Message: 7 Date: Tue, 22 Jun 2010 16:10:12 -0400 From: "Martha Ward" Subject: [Histonet] Glut1 antibody To: Message-ID: <61135F0455D33347B5AAE209B903A30433DEBA4A@EXCHVS2.medctr.ad.wfubmc.edu> Content-Type: text/plain; charset="us-ascii" I have been using anti-human Glut-1 from Dako for several years but when I went to order it I was told it was discontinued. Does anyone have a suggestion of where I can locate this antibody? Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 28 **************************************** Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* From sjchtascp <@t> yahoo.com Thu Jun 24 12:22:23 2010 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Thu Jun 24 12:22:27 2010 Subject: [Histonet] (no subject) Message-ID: <640970.28465.qm@web38207.mail.mud.yahoo.com> Looking for work in Histology Lab.? Madison, Milwaukee, So. WI.? No.Ill From dmccaig <@t> ckha.on.ca Thu Jun 24 12:47:44 2010 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Thu Jun 24 12:47:54 2010 Subject: [Histonet] AB-PAS for Barrett's Esophagus Message-ID: Can anyone share with me their procedure for AB-PAS for Barrett's Esophagus. With thanks Diana From lteves <@t> uhnresearch.ca Thu Jun 24 12:49:37 2010 From: lteves <@t> uhnresearch.ca (Lucy Teves) Date: Thu Jun 24 12:54:36 2010 Subject: [Histonet] TP1050 processing protocol Message-ID: <4C239AB1.4020108@uhnresearch.ca> I work in a small research lab in Toronto. Just purchased a used TP1050 tissue processor. The processing schedule is very different from my old one histomatic tissue processor that recently died. I have been trying for weeks to adapt my old processing schedule to the "new" processor. I was hoping to get some help with this. I would love established protocols for * 10 % NBF fixed non human primate coronal 3mm thick brain sections * 10% NBF fixed rat coronal 2mm thick brain sections * 10% NBF fixed mouse coronal 2mm thick brain sections * 10% NBF fixed mouse whole heart Suggestions would be very much appreciated as well. Thanks in advance L. -- From Betsy.Hoffman <@t> comphealth.com Thu Jun 24 13:11:25 2010 From: Betsy.Hoffman <@t> comphealth.com (Betsy Hoffman) Date: Thu Jun 24 13:11:58 2010 Subject: [Histonet] HOT JOB! Histotech needed for day shift in Eastern Texas- New Grads apply now! Message-ID: <585C6608F313C34D8979DD7A9CD38DFA056EDEB2@vslcexmbp02.mychg.com> Thought you'd be interested in seeing this HOT job opportunity currently available through CompHealth. If you're interested in finding out more...call me!! Histotech needed for day shift in Eastern Texas- New Grads apply now! ASCP/HTL or HT needed to work day shift at this top rated facility. Our friendly people, rich history, and comfortable lifestyle make this a great place to live and work. This is a great opportunity for New Grads. Relo allowance offered. Processes all cytology and pathology specimens under the supervision of the Pathologist. High School Graduate plus must have graduated from an accredited school of Histotechnology- Must have specific technical knowledge of laboratory testing methods & of appropriate theory **If this doesn't appeal to you...maybe you know someone else who's looking....refer them to me and we'll pay you a referral fee Sincerely, Betsy Hoffman Search Consultant, Lab Sciences CompHealth Permanent Placement 6451 North Federal Highway, Suite 702 Ft. Lauderdale, FL 33308 Direct: 1-954-837-2622| Office: 1-866-782-9029, X2622| Fax: 1-800-420-2329 betsy.hoffman@comphealth.com Search Jobs Online | Visit us at www.comphealth.com Learn more about our award-winning company and the people behind it! ASK ABOUT OUR $250 BONUS FOR REFERRALS!!! Customer service is the key to success. Are you satisfied with your experience? Send your comments to my manager at carlos.hagler@comphealth.com This is a commercial email from CompHealth. If you do not want to receive future emails from CompHealth, please reply to the sender of this email and ask to be removed. From histology <@t> medsurgpath.com Thu Jun 24 13:30:39 2010 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Thu Jun 24 13:30:46 2010 Subject: [Histonet] parasites in stool Message-ID: <466be11eada0e1004684dd22d5b87d11.squirrel@webmail.integra.net> Hi all, I have received some parasitic stool samples, one fixed in formalin, the other in PVU. My tasks are: 1) to try to remove as much debris from the stool sample as possible while keeping the parasites intact (I need to try to filter it without harming the very fragile organisms) 2) once I am able to separate the organisms, I need to stain them. We have tried using a Gram stain and it does not work, we are thinking we should try a Giemsa and an H&E. If anyone has ever worked with anything similar, I would really appreciate some feedback, suggestions, advice with how to filter these samples and stain them. Regards, Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 From histology <@t> medsurgpath.com Thu Jun 24 13:34:53 2010 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Thu Jun 24 13:35:03 2010 Subject: [Histonet] AB-PAS for Barrett's Esophagus In-Reply-To: References: Message-ID: <7bf6eb0a40a7e2b24bdae795073d0d93.squirrel@webmail.integra.net> We use the AmericanMasterTech Alcian Blue pH 2.5 and use this procedure: Place slides in 3% Acetic Acid for 3 minutes. Place slides in Alcian Blue for 30 minutes. Rinse slides in water Place slides in 0.5% Periodic Acid for 10 minutes. Rinse slides in water Place slides in Schiff Reagent for 15 minutes Rinse slides in HOT water for 5 minutes. Counter stain in Hematoxylin for 2 minutes. Rinse slides in water, Dehydrate slides through Absolute Alcohol, Clear through Xylene Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > Can anyone share with me their procedure for AB-PAS for Barrett's > Esophagus. > With thanks > > Diana > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Rhonda.Hartz <@t> saskatoonhealthregion.ca Thu Jun 24 13:35:53 2010 From: Rhonda.Hartz <@t> saskatoonhealthregion.ca (Hartz, Rhonda SktnHR) Date: Thu Jun 24 13:49:55 2010 Subject: [Histonet] HER2 - Automated Slide Reade Message-ID: Does anyone use an automated slide reader for HER2 slides? Possibly in the market and would really appreciate some feedback on what system and associated comments? Thanks! Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca From jmcgough <@t> clinlab.com Thu Jun 24 14:29:37 2010 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Thu Jun 24 14:29:45 2010 Subject: [Histonet] AB-PAS for Barrett's Esophagus In-Reply-To: Message-ID: 1% Periodic Acid Solution 0.5% Sodium Metabisulfite Periodic Acid....1 gm Sodium metabisulfite.0.5 gm Distilled water..100 ml Distilled water....100 ml .1N HCL 3% Glacial Acetic Acid Conc. (10N) HCL.1 ml Conc. Glacial Acetic Acid..3 ml Distilled Water.100 ml Distilled water......100 ml Alcian Blue Solution pH 1.0 Alcian Blue Solution pH 2.5 Alcian Blue 8GX...1.0 gm Alcian Blue 8GX....1.0 gm 0.1N HCL....100 ml 3% Glacial Acetic Acid.100 ml Adjust pH to 2.5. Add a few Schiff's Reagent Thymol crystals for preservative. Purchased Commercially (ready to use) PROCEDURAL NOTES 1. pH 2.5 is routinely used in our laboratory. Use pH 1.0 only if the pathologist specifically requests you to do so. 2. Filter Alcian Blue solutions before use. 3. Use an aliquot from the stock bottle of Schiff's reagent. This may be reused but do not return to stock bottle. Change frequently and discard if solution is pink. PROCEDURE 1. Deparaffinize and hydrate to distilled water. 2. Place slides in freshly filtered Alcian Blue solution for 30 minutes. 3. If using Alcian Blue solution 2.5 - Wash in running water for 5 minutes. If using Alcian Blue solution 1.0 - Blot section dry with fine filter paper. 4. Oxidize in Periodic Acid solution for 10 minutes. 5. Wash in running water for 5 minutes. 6. Place slides in Schiff's reagent for 10 minutes. 7. Rinse in Sodium Metabisulfite solution, 3 changes; 2 minutes each. 8. Wash in running water for 10 minutes. 9. Dehydrate in 95% alcohol, 100% alcohol, clear in xylene, two changes each. 10. Coverslip. RESULTS 1. pH 2.5 (acid group) -Exclusively acid substances (various connective tissue mucins) - blue -Neutral polysaccharides (glycogen) - magenta -Both Alcian blue and PAS, yielding varying shades of purple to deep blue, color most epithelial mucins and cartilage ground substance. -Cell bodies of fungi - red to purple -Mucoid capsules - blue -Other features resemble PAS stain 2. pH 1.0 (sulphate group) Sulfated mucosubstances - blue REFERENCE 1. Lev, R., Spicer, S.S.;J. Histochem. Cystochem. 12:309,1964. Copyright by Williams and Wilkins Co. 2. C.F.A. Culling; Handbook of Histopathological and Histochemical Techniques, 3rh e. 1974. Butterworth. 3. Preece, Ann: Manual for Histologic Technicians 2nd ed. 1965. 4. AFIP Manual For Histologic Stain Methods, 3rd ed. 5. Schenk, E.A., M.D. and Mowry, R.W., MD>D, Journal of Histotechnology Vol. 6#2, June 1983. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana McCaig Sent: Thursday, June 24, 2010 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AB-PAS for Barrett's Esophagus Can anyone share with me their procedure for AB-PAS for Barrett's Esophagus. With thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Thu Jun 24 15:11:13 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Thu Jun 24 15:11:43 2010 Subject: [Histonet] PREFILLED FORMALIN W/ SEAL Message-ID: THANKS EVERYBODY I have found someone to help!! Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From canyon <@t> path-tec.com Thu Jun 24 15:23:26 2010 From: canyon <@t> path-tec.com (Canyon Bowie) Date: Thu Jun 24 15:24:08 2010 Subject: [Histonet] Prefilled Formalin Jars In-Reply-To: References: Message-ID: <034801cb13db$1a759470$4f60bd50$@com> Hi Kathy, We provide prefills and can customize them with your private labeling, security seals, color coded lids and accessioning and/or bar-coding. Feel free to contact me for more information. Canyon Bowie Check out our new website!! www.path-tec.com Path-Tec w. 706.507.1575 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Wednesday, June 23, 2010 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prefilled Formalin Jars Hi histonetters: Was wondering if anybody knew of a vendor that had prefilled formalin jars that have a seal on them? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From schaundrawalton <@t> yahoo.com Thu Jun 24 18:05:22 2010 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Thu Jun 24 18:05:26 2010 Subject: [Histonet] Maintenence HELP! Message-ID: <922827.90102.qm@web120615.mail.ne1.yahoo.com> Hey Histonetters! ? I have some equipment I need looked at.? Can anyone recommend a service company/tech in the Orlando area?? ? -Schaundra K. Walton BS HTL(ASCP) Histotechnology Program Director Keiser University Orlando, FL From jcarpenter764 <@t> aol.com Fri Jun 25 09:33:58 2010 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Fri Jun 25 09:34:33 2010 Subject: [Histonet] Histology techs grossing in specimens??? Message-ID: <8CCE28A94BC057C-924-9314@webmail-m091.sysops.aol.com> Trying to get some feed back on histology techs grossing in for the doctors......My main question would be the training involved? Can anyone just start to gross in biopsies and small specimens. What does it include and what sort of education is involved if any???? Thanks! From jcarpenter764 <@t> aol.com Fri Jun 25 09:33:58 2010 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Fri Jun 25 09:34:36 2010 Subject: [Histonet] Histology techs grossing in specimens??? Message-ID: <8CCE28A94BC057C-924-9314@webmail-m091.sysops.aol.com> Trying to get some feed back on histology techs grossing in for the doctors......My main question would be the training involved? Can anyone just start to gross in biopsies and small specimens. What does it include and what sort of education is involved if any???? Thanks! From JCBRITTON <@t> Cheshire-Med.COM Fri Jun 25 09:46:15 2010 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Fri Jun 25 09:46:26 2010 Subject: [Histonet] (no subject) In-Reply-To: <73A7ED895EE0C24D9267ED814911DF1912B754B2@exchange.cmc-nh.org> References: <73A7ED895EE0C24D9267ED814911DF1912B754B2@exchange.cmc-nh.org> Message-ID: We retain our containers and specimens for 3 weeks. Josie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Wednesday, June 23, 2010 5:51 PM To: Hartz, Rhonda SktnHR; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) We retain them until the specimen is signed out, usually no more than 3 days. This has been helpful if the specimen container labeling is called into question either by the pathologist (because the cellular profile does not match what the specimen source indicates) or the clinician. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hartz, Rhonda SktnHR Sent: Tuesday, June 22, 2010 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From sbaldwin <@t> mhhcc.org Fri Jun 25 10:38:34 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Fri Jun 25 10:39:07 2010 Subject: [Histonet] ARTICLE Message-ID: Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called "Workplace Violence in the Laboratory" Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From histology <@t> medsurgpath.com Fri Jun 25 10:52:58 2010 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Fri Jun 25 10:53:11 2010 Subject: [Histonet] Histology techs grossing in specimens??? In-Reply-To: <8CCE28A94BC057C-924-9314@webmail-m091.sysops.aol.com> References: <8CCE28A94BC057C-924-9314@webmail-m091.sysops.aol.com> Message-ID: We do all of our grossing and use the CLIA regulations: TESTING PERSONNEL (42 CFR 493 1489) 1. Licensed MD, DO or DPM. 2. Doctorate, master's, or bachelor's in laboratory science. 3. Education and training equivalent to an associate degree in a laboratory science or medical laboratory technology that includes at least 60 semester hours including 24 semester hrs of medical lab technology and at least 3 months training in each specialty in which high complexity testing is performed; or, 60 semester hrs including 24 hrs of science that includes 6 hrs chemistry, 6 hrs biology, and 12 hrs chemistry, biology or, medical lab tech in any combination and laboratory training that includes either: completion of a clinical lab training program and at least 3 months training in each specialty in which high complexity testing is performed. It is my understanding that if there is no cutting involved (i.e. counting and measuring GI biopsies) it is not considered a "high complexity" task and anyone in the lab would be able to "gross" in that regard. Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > > > > Trying to get some feed back on histology techs grossing in for the > doctors......My main question would be the training involved? Can anyone > just start to gross in biopsies and small specimens. What does it include > and what sort of education is involved if any???? Thanks! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histology <@t> medsurgpath.com Fri Jun 25 10:52:58 2010 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Fri Jun 25 10:53:18 2010 Subject: [Histonet] Histology techs grossing in specimens??? In-Reply-To: <8CCE28A94BC057C-924-9314@webmail-m091.sysops.aol.com> References: <8CCE28A94BC057C-924-9314@webmail-m091.sysops.aol.com> Message-ID: We do all of our grossing and use the CLIA regulations: TESTING PERSONNEL (42 CFR 493 1489) 1. Licensed MD, DO or DPM. 2. Doctorate, master's, or bachelor's in laboratory science. 3. Education and training equivalent to an associate degree in a laboratory science or medical laboratory technology that includes at least 60 semester hours including 24 semester hrs of medical lab technology and at least 3 months training in each specialty in which high complexity testing is performed; or, 60 semester hrs including 24 hrs of science that includes 6 hrs chemistry, 6 hrs biology, and 12 hrs chemistry, biology or, medical lab tech in any combination and laboratory training that includes either: completion of a clinical lab training program and at least 3 months training in each specialty in which high complexity testing is performed. It is my understanding that if there is no cutting involved (i.e. counting and measuring GI biopsies) it is not considered a "high complexity" task and anyone in the lab would be able to "gross" in that regard. Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > > > > Trying to get some feed back on histology techs grossing in for the > doctors......My main question would be the training involved? Can anyone > just start to gross in biopsies and small specimens. What does it include > and what sort of education is involved if any???? Thanks! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jackie.O'Connor <@t> abbott.com Fri Jun 25 10:59:06 2010 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Jun 25 10:59:58 2010 Subject: [Histonet] ARTICLE In-Reply-To: References: Message-ID: No - but I could write one. From: "Sara Baldwin/mhhcc.org" To: Date: 06/25/2010 10:53 AM Subject: [Histonet] ARTICLE Sent by: histonet-bounces@lists.utsouthwestern.edu Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called "Workplace Violence in the Laboratory" Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri Jun 25 11:31:38 2010 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Jun 25 11:33:29 2010 Subject: [Histonet] ARTICLE In-Reply-To: References: Message-ID: <85F2E7DD5D91744D831A66E44D718B9A138BDC62@fhovxchmb7001.ADVENTISTCORP.NET> http://laboratorian.advanceweb.com/Editorial/Search/Searchresult.aspx?KW=violence+in+the+workplace ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org [sbaldwin@mhhcc.org] Sent: Friday, June 25, 2010 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ARTICLE Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called "Workplace Violence in the Laboratory" Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From brandihiggins <@t> gmail.com Fri Jun 25 11:33:37 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Fri Jun 25 11:33:43 2010 Subject: [Histonet] Prepared Solutions Message-ID: Hello All, I work in a small lab where we purchase most of our chemicals already prepared. The only solutions we routinely prepare ourselves are our acid alcohol and acid water for our staining procedures, Carnoy's Fixative (since it needs to be fresh) and our 50%, 70% etc alcohol solutions for our Pap stain and for our processing machine. I would also like to start preparing our 1% and 3% acetic acid solutions instead of purchasing them since I have glacial acetic acid on hand anyway for our Carnoy's Fixative (not that the acetic acid acid solutions are expensive but every dollar counts, plus the shipping charges always shock me). My questions are 1 - in your labs, do you have a policy with instructions on how to prepare each solution? (eg 1 ml acetic acid diluted with water to 100 ml to prepate 1% Acetic Acid Aq and is it neccessary to say 500 ml water and 500 ml reagent alcohol to form 50% alcohol) 2 - when you label the solutions you prepare, do you transfer the lot numbers and expiration dates of the chemicals that were used to make the solutions onto the new chemical bottles and use the expiration date of the concentrated chemical as the expiration date of the prepared chemical? Thanks in advance for all input. Im always amazed at how fast, how many, and how thorough/helpful the responses are! Brandi Higgins, BS, HT(ASCP) From Bill.Tench <@t> pph.org Fri Jun 25 12:31:48 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Fri Jun 25 12:31:53 2010 Subject: [Histonet] Re Grossing assistants Message-ID: <2820431BF953BB4DA3E9E1A5882265FD028631BB@MAIL1.pph.local> I would suggest that you review the archives on this topic. There has been much discussion recently. The short answer is that ANYONE performing ANY grossing in the AP laboratory must meet the requirements for high complexity testing. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- From Sheri.Meilus <@t> va.gov Fri Jun 25 12:48:51 2010 From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.) Date: Fri Jun 25 12:49:04 2010 Subject: [Histonet] Grossing Personnel Requirements In-Reply-To: References: Message-ID: Hi All, I hope this will help to clarify the new ruling passed down from CAP regarding personnel requirements for grossing specimens. Historically, CAP differentiated between "grossing" and "processing". Grossing required orientation and/or dissection of the specimen and was considered high complexity testing. In contrast, "processing" did not require specific orientation and minimal cutting and was not considered high complexity. However, CAP now considers grossing and processing to be in one category and is all high complexity. I will excerpt the CAP alert and other pertinent information below: March 31, 2010 Attention Anatomic Pathology Laboratories: In preparation for the release of the 2010 CAP Checklist Edition in June of this year, CAP is notifying all accredited anatomic pathology laboratories of a revised checklist requirement that may have an impact on your laboratory's staffing. The revisions will require that all non-pathologist individuals who perform macroscopic tissue examinations meet the personnel requirements for high complexity testing in accordance with CLIA. This interpretation of the CLIA requirement was recently provided to CAP from CMS. As a service to CAP Accredited laboratories, the CAP offers compliance alerts to help your laboratory maintain continuous compliance. Previously, the Anatomic Pathology checklist differentiated two levels of macroscopic examination, "processing" and "grossing." In this context, "processing" means macroscopic examination of small specimens not requiring knowledge of anatomy, which are entirely submitted for microscopic examination, while "grossing" means macroscopic examination of more complex specimens. Unlike individuals who performed grossing, individuals who performed "processing" were not required to be qualified as high complexity testing personnel. In the 2010 checklist edition, the concept of macroscopic tissue "processing" will no longer be recognized. All macroscopic tissue examinations will be considered to be "grossing." Therefore, any individual who performs macroscopic tissue examinations must be a pathologist, pathology resident, or an individual qualified to perform high complexity testing under the supervision of a pathologist (refer to ANP.11610, below). Please contact the Laboratory Accreditation Program at (800) 323-4040, option 1, then 4, or 1-847-832-7000, or by email if you have any questions. ________________________________________ ANP.11610 Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. The CLIA regulations on high complexity testing personnel may be found at HC Testing Personnel. In addition, the CLIA regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at the above Web address and at Grandfathered Exceptions. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. Sorry it is rather lengthy, but it should put to rest most of your questions regarding the new regulation and how it impacts labs that utilize non-Pathologists for grossing surgical specimens. Best regards, Sheri S Sheri Meilus Anatomic Pathology Supervisor Bay Pines VAHC Building 100 Room 2B-126 727-398-6661 ext 4596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, June 25, 2010 1:22 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 32 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. (no subject) (Steven Coakley) 2. AB-PAS for Barrett's Esophagus (Diana McCaig) 3. TP1050 processing protocol (Lucy Teves) 4. HOT JOB! Histotech needed for day shift in Eastern Texas- New Grads apply now! (Betsy Hoffman) 5. parasites in stool (Katelin Lester) 6. Re: AB-PAS for Barrett's Esophagus (Katelin Lester) 7. HER2 - Automated Slide Reade (Hartz, Rhonda SktnHR) 8. RE: AB-PAS for Barrett's Esophagus (Jason McGough) 9. PREFILLED FORMALIN W/ SEAL (Sara Baldwin/mhhcc.org) 10. RE: Prefilled Formalin Jars (Canyon Bowie) 11. Maintenence HELP! (Schaundra Walton) 12. Histology techs grossing in specimens??? (jcarpenter764@aol.com) 13. Histology techs grossing in specimens??? (jcarpenter764@aol.com) 14. RE: (no subject) (Josie Britton) 15. ARTICLE (Sara Baldwin/mhhcc.org) 16. Re: Histology techs grossing in specimens??? (Katelin Lester) 17. Re: Histology techs grossing in specimens??? (Katelin Lester) 18. Re: ARTICLE (Jackie M O'Connor) 19. RE: ARTICLE (Bonner, Janet) 20. Prepared Solutions (Brandi Higgins) ---------------------------------------------------------------------- Message: 1 Date: Thu, 24 Jun 2010 10:22:23 -0700 (PDT) From: Steven Coakley Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: <640970.28465.qm@web38207.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Looking for work in Histology Lab.? Madison, Milwaukee, So. WI.? No.Ill ------------------------------ Message: 2 Date: Thu, 24 Jun 2010 13:47:44 -0400 From: "Diana McCaig" Subject: [Histonet] AB-PAS for Barrett's Esophagus To: Message-ID: Content-Type: text/plain; charset="us-ascii" Can anyone share with me their procedure for AB-PAS for Barrett's Esophagus. With thanks Diana ------------------------------ Message: 3 Date: Thu, 24 Jun 2010 13:49:37 -0400 From: Lucy Teves Subject: [Histonet] TP1050 processing protocol To: histonet@lists.utsouthwestern.edu Message-ID: <4C239AB1.4020108@uhnresearch.ca> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I work in a small research lab in Toronto. Just purchased a used TP1050 tissue processor. The processing schedule is very different from my old one histomatic tissue processor that recently died. I have been trying for weeks to adapt my old processing schedule to the "new" processor. I was hoping to get some help with this. I would love established protocols for * 10 % NBF fixed non human primate coronal 3mm thick brain sections * 10% NBF fixed rat coronal 2mm thick brain sections * 10% NBF fixed mouse coronal 2mm thick brain sections * 10% NBF fixed mouse whole heart Suggestions would be very much appreciated as well. Thanks in advance L. -- ------------------------------ Message: 4 Date: Thu, 24 Jun 2010 18:11:25 +0000 From: Betsy Hoffman Subject: [Histonet] HOT JOB! Histotech needed for day shift in Eastern Texas- New Grads apply now! To: "histonet@lists.utsouthwestern.edu" Message-ID: <585C6608F313C34D8979DD7A9CD38DFA056EDEB2@vslcexmbp02.mychg.com> Content-Type: text/plain; charset="us-ascii" Thought you'd be interested in seeing this HOT job opportunity currently available through CompHealth. If you're interested in finding out more...call me!! Histotech needed for day shift in Eastern Texas- New Grads apply now! ASCP/HTL or HT needed to work day shift at this top rated facility. Our friendly people, rich history, and comfortable lifestyle make this a great place to live and work. This is a great opportunity for New Grads. Relo allowance offered. Processes all cytology and pathology specimens under the supervision of the Pathologist. High School Graduate plus must have graduated from an accredited school of Histotechnology- Must have specific technical knowledge of laboratory testing methods & of appropriate theory **If this doesn't appeal to you...maybe you know someone else who's looking....refer them to me and we'll pay you a referral fee Sincerely, Betsy Hoffman Search Consultant, Lab Sciences CompHealth Permanent Placement 6451 North Federal Highway, Suite 702 Ft. Lauderdale, FL 33308 Direct: 1-954-837-2622| Office: 1-866-782-9029, X2622| Fax: 1-800-420-2329 betsy.hoffman@comphealth.com Search Jobs Online | Visit us at www.comphealth.com Learn more about our award-winning company and the people behind it! ASK ABOUT OUR $250 BONUS FOR REFERRALS!!! Customer service is the key to success. Are you satisfied with your experience? Send your comments to my manager at carlos.hagler@comphealth.com This is a commercial email from CompHealth. If you do not want to receive future emails from CompHealth, please reply to the sender of this email and ask to be removed. ------------------------------ Message: 5 Date: Thu, 24 Jun 2010 11:30:39 -0700 From: "Katelin Lester" Subject: [Histonet] parasites in stool To: "histonet" Message-ID: <466be11eada0e1004684dd22d5b87d11.squirrel@webmail.integra.net> Content-Type: text/plain;charset=iso-8859-1 Hi all, I have received some parasitic stool samples, one fixed in formalin, the other in PVU. My tasks are: 1) to try to remove as much debris from the stool sample as possible while keeping the parasites intact (I need to try to filter it without harming the very fragile organisms) 2) once I am able to separate the organisms, I need to stain them. We have tried using a Gram stain and it does not work, we are thinking we should try a Giemsa and an H&E. If anyone has ever worked with anything similar, I would really appreciate some feedback, suggestions, advice with how to filter these samples and stain them. Regards, Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 ------------------------------ Message: 6 Date: Thu, 24 Jun 2010 11:34:53 -0700 From: "Katelin Lester" Subject: Re: [Histonet] AB-PAS for Barrett's Esophagus To: "Diana McCaig" Cc: histonet@lists.utsouthwestern.edu Message-ID: <7bf6eb0a40a7e2b24bdae795073d0d93.squirrel@webmail.integra.net> Content-Type: text/plain;charset=iso-8859-1 We use the AmericanMasterTech Alcian Blue pH 2.5 and use this procedure: Place slides in 3% Acetic Acid for 3 minutes. Place slides in Alcian Blue for 30 minutes. Rinse slides in water Place slides in 0.5% Periodic Acid for 10 minutes. Rinse slides in water Place slides in Schiff Reagent for 15 minutes Rinse slides in HOT water for 5 minutes. Counter stain in Hematoxylin for 2 minutes. Rinse slides in water, Dehydrate slides through Absolute Alcohol, Clear through Xylene Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > Can anyone share with me their procedure for AB-PAS for Barrett's > Esophagus. > With thanks > > Diana > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Thu, 24 Jun 2010 12:35:53 -0600 From: "Hartz, Rhonda SktnHR" Subject: [Histonet] HER2 - Automated Slide Reade To: Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone use an automated slide reader for HER2 slides? Possibly in the market and would really appreciate some feedback on what system and associated comments? Thanks! Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca ------------------------------ Message: 8 Date: Thu, 24 Jun 2010 13:29:37 -0600 From: "Jason McGough" Subject: RE: [Histonet] AB-PAS for Barrett's Esophagus To: "Diana McCaig" , Message-ID: Content-Type: text/plain; charset="us-ascii" 1% Periodic Acid Solution 0.5% Sodium Metabisulfite Periodic Acid....1 gm Sodium metabisulfite.0.5 gm Distilled water..100 ml Distilled water....100 ml .1N HCL 3% Glacial Acetic Acid Conc. (10N) HCL.1 ml Conc. Glacial Acetic Acid..3 ml Distilled Water.100 ml Distilled water......100 ml Alcian Blue Solution pH 1.0 Alcian Blue Solution pH 2.5 Alcian Blue 8GX...1.0 gm Alcian Blue 8GX....1.0 gm 0.1N HCL....100 ml 3% Glacial Acetic Acid.100 ml Adjust pH to 2.5. Add a few Schiff's Reagent Thymol crystals for preservative. Purchased Commercially (ready to use) PROCEDURAL NOTES 1. pH 2.5 is routinely used in our laboratory. Use pH 1.0 only if the pathologist specifically requests you to do so. 2. Filter Alcian Blue solutions before use. 3. Use an aliquot from the stock bottle of Schiff's reagent. This may be reused but do not return to stock bottle. Change frequently and discard if solution is pink. PROCEDURE 1. Deparaffinize and hydrate to distilled water. 2. Place slides in freshly filtered Alcian Blue solution for 30 minutes. 3. If using Alcian Blue solution 2.5 - Wash in running water for 5 minutes. If using Alcian Blue solution 1.0 - Blot section dry with fine filter paper. 4. Oxidize in Periodic Acid solution for 10 minutes. 5. Wash in running water for 5 minutes. 6. Place slides in Schiff's reagent for 10 minutes. 7. Rinse in Sodium Metabisulfite solution, 3 changes; 2 minutes each. 8. Wash in running water for 10 minutes. 9. Dehydrate in 95% alcohol, 100% alcohol, clear in xylene, two changes each. 10. Coverslip. RESULTS 1. pH 2.5 (acid group) -Exclusively acid substances (various connective tissue mucins) - blue -Neutral polysaccharides (glycogen) - magenta -Both Alcian blue and PAS, yielding varying shades of purple to deep blue, color most epithelial mucins and cartilage ground substance. -Cell bodies of fungi - red to purple -Mucoid capsules - blue -Other features resemble PAS stain 2. pH 1.0 (sulphate group) Sulfated mucosubstances - blue REFERENCE 1. Lev, R., Spicer, S.S.;J. Histochem. Cystochem. 12:309,1964. Copyright by Williams and Wilkins Co. 2. C.F.A. Culling; Handbook of Histopathological and Histochemical Techniques, 3rh e. 1974. Butterworth. 3. Preece, Ann: Manual for Histologic Technicians 2nd ed. 1965. 4. AFIP Manual For Histologic Stain Methods, 3rd ed. 5. Schenk, E.A., M.D. and Mowry, R.W., MD>D, Journal of Histotechnology Vol. 6#2, June 1983. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana McCaig Sent: Thursday, June 24, 2010 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AB-PAS for Barrett's Esophagus Can anyone share with me their procedure for AB-PAS for Barrett's Esophagus. With thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 24 Jun 2010 16:11:13 -0400 From: "Sara Baldwin/mhhcc.org" Subject: [Histonet] PREFILLED FORMALIN W/ SEAL To: Message-ID: Content-Type: text/plain; charset=ISO-8859-1 THANKS EVERYBODY I have found someone to help!! Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ------------------------------ Message: 10 Date: Thu, 24 Jun 2010 16:23:26 -0400 From: "Canyon Bowie" Subject: RE: [Histonet] Prefilled Formalin Jars To: "'Sara Baldwin/mhhcc.org'" , Message-ID: <034801cb13db$1a759470$4f60bd50$@com> Content-Type: text/plain; charset="us-ascii" Hi Kathy, We provide prefills and can customize them with your private labeling, security seals, color coded lids and accessioning and/or bar-coding. Feel free to contact me for more information. Canyon Bowie Check out our new website!! www.path-tec.com Path-Tec w. 706.507.1575 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Wednesday, June 23, 2010 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prefilled Formalin Jars Hi histonetters: Was wondering if anybody knew of a vendor that had prefilled formalin jars that have a seal on them? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 24 Jun 2010 16:05:22 -0700 (PDT) From: Schaundra Walton Subject: [Histonet] Maintenence HELP! To: histonet@lists.utsouthwestern.edu Message-ID: <922827.90102.qm@web120615.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hey Histonetters! ? I have some equipment I need looked at.? Can anyone recommend a service company/tech in the Orlando area?? ? -Schaundra K. Walton BS HTL(ASCP) Histotechnology Program Director Keiser University Orlando, FL ------------------------------ Message: 12 Date: Fri, 25 Jun 2010 10:33:58 -0400 From: jcarpenter764@aol.com Subject: [Histonet] Histology techs grossing in specimens??? To: histonet@lists.utsouthwestern.edu, histonet@pathology.swmed.edu Message-ID: <8CCE28A94BC057C-924-9314@webmail-m091.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Trying to get some feed back on histology techs grossing in for the doctors......My main question would be the training involved? Can anyone just start to gross in biopsies and small specimens. What does it include and what sort of education is involved if any???? Thanks! ------------------------------ Message: 13 Date: Fri, 25 Jun 2010 10:33:58 -0400 From: jcarpenter764@aol.com Subject: [Histonet] Histology techs grossing in specimens??? To: histonet@lists.utsouthwestern.edu, histonet@pathology.swmed.edu Message-ID: <8CCE28A94BC057C-924-9314@webmail-m091.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Trying to get some feed back on histology techs grossing in for the doctors......My main question would be the training involved? Can anyone just start to gross in biopsies and small specimens. What does it include and what sort of education is involved if any???? Thanks! ------------------------------ Message: 14 Date: Fri, 25 Jun 2010 10:46:15 -0400 From: "Josie Britton" Subject: RE: [Histonet] (no subject) To: "Feher, Stephen" , "Hartz, Rhonda SktnHR" , Message-ID: Content-Type: text/plain; charset="us-ascii" We retain our containers and specimens for 3 weeks. Josie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Wednesday, June 23, 2010 5:51 PM To: Hartz, Rhonda SktnHR; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) We retain them until the specimen is signed out, usually no more than 3 days. This has been helpful if the specimen container labeling is called into question either by the pathologist (because the cellular profile does not match what the specimen source indicates) or the clinician. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hartz, Rhonda SktnHR Sent: Tuesday, June 22, 2010 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ------------------------------ Message: 15 Date: Fri, 25 Jun 2010 11:38:34 -0400 From: "Sara Baldwin/mhhcc.org" Subject: [Histonet] ARTICLE To: Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called "Workplace Violence in the Laboratory" Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ------------------------------ Message: 16 Date: Fri, 25 Jun 2010 08:52:58 -0700 From: "Katelin Lester" Subject: Re: [Histonet] Histology techs grossing in specimens??? To: jcarpenter764@aol.com Cc: histonet@lists.utsouthwestern.edu, histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain;charset=iso-8859-1 We do all of our grossing and use the CLIA regulations: TESTING PERSONNEL (42 CFR 493 1489) 1. Licensed MD, DO or DPM. 2. Doctorate, master's, or bachelor's in laboratory science. 3. Education and training equivalent to an associate degree in a laboratory science or medical laboratory technology that includes at least 60 semester hours including 24 semester hrs of medical lab technology and at least 3 months training in each specialty in which high complexity testing is performed; or, 60 semester hrs including 24 hrs of science that includes 6 hrs chemistry, 6 hrs biology, and 12 hrs chemistry, biology or, medical lab tech in any combination and laboratory training that includes either: completion of a clinical lab training program and at least 3 months training in each specialty in which high complexity testing is performed. It is my understanding that if there is no cutting involved (i.e. counting and measuring GI biopsies) it is not considered a "high complexity" task and anyone in the lab would be able to "gross" in that regard. Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > > > > Trying to get some feed back on histology techs grossing in for the > doctors......My main question would be the training involved? Can anyone > just start to gross in biopsies and small specimens. What does it include > and what sort of education is involved if any???? Thanks! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 17 Date: Fri, 25 Jun 2010 08:52:58 -0700 From: "Katelin Lester" Subject: Re: [Histonet] Histology techs grossing in specimens??? To: jcarpenter764@aol.com Cc: histonet@lists.utsouthwestern.edu, histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain;charset=iso-8859-1 We do all of our grossing and use the CLIA regulations: TESTING PERSONNEL (42 CFR 493 1489) 1. Licensed MD, DO or DPM. 2. Doctorate, master's, or bachelor's in laboratory science. 3. Education and training equivalent to an associate degree in a laboratory science or medical laboratory technology that includes at least 60 semester hours including 24 semester hrs of medical lab technology and at least 3 months training in each specialty in which high complexity testing is performed; or, 60 semester hrs including 24 hrs of science that includes 6 hrs chemistry, 6 hrs biology, and 12 hrs chemistry, biology or, medical lab tech in any combination and laboratory training that includes either: completion of a clinical lab training program and at least 3 months training in each specialty in which high complexity testing is performed. It is my understanding that if there is no cutting involved (i.e. counting and measuring GI biopsies) it is not considered a "high complexity" task and anyone in the lab would be able to "gross" in that regard. Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > > > > Trying to get some feed back on histology techs grossing in for the > doctors......My main question would be the training involved? Can anyone > just start to gross in biopsies and small specimens. What does it include > and what sort of education is involved if any???? Thanks! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 18 Date: Fri, 25 Jun 2010 10:59:06 -0500 From: Jackie M O'Connor Subject: Re: [Histonet] ARTICLE To: "Sara Baldwin/mhhcc.org" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" No - but I could write one. From: "Sara Baldwin/mhhcc.org" To: Date: 06/25/2010 10:53 AM Subject: [Histonet] ARTICLE Sent by: histonet-bounces@lists.utsouthwestern.edu Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called "Workplace Violence in the Laboratory" Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Fri, 25 Jun 2010 12:31:38 -0400 From: "Bonner, Janet" Subject: RE: [Histonet] ARTICLE To: "Sara Baldwin/mhhcc.org" , "histonet@lists.utsouthwestern.edu" Message-ID: <85F2E7DD5D91744D831A66E44D718B9A138BDC62@fhovxchmb7001.ADVENTISTCORP.NET> Content-Type: text/plain; charset=us-ascii http://laboratorian.advanceweb.com/Editorial/Search/Searchresult.aspx?KW=violence+in+the+workplace ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org [sbaldwin@mhhcc.org] Sent: Friday, June 25, 2010 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ARTICLE Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called "Workplace Violence in the Laboratory" Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 20 Date: Fri, 25 Jun 2010 12:33:37 -0400 From: Brandi Higgins Subject: [Histonet] Prepared Solutions To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello All, I work in a small lab where we purchase most of our chemicals already prepared. The only solutions we routinely prepare ourselves are our acid alcohol and acid water for our staining procedures, Carnoy's Fixative (since it needs to be fresh) and our 50%, 70% etc alcohol solutions for our Pap stain and for our processing machine. I would also like to start preparing our 1% and 3% acetic acid solutions instead of purchasing them since I have glacial acetic acid on hand anyway for our Carnoy's Fixative (not that the acetic acid acid solutions are expensive but every dollar counts, plus the shipping charges always shock me). My questions are 1 - in your labs, do you have a policy with instructions on how to prepare each solution? (eg 1 ml acetic acid diluted with water to 100 ml to prepate 1% Acetic Acid Aq and is it neccessary to say 500 ml water and 500 ml reagent alcohol to form 50% alcohol) 2 - when you label the solutions you prepare, do you transfer the lot numbers and expiration dates of the chemicals that were used to make the solutions onto the new chemical bottles and use the expiration date of the concentrated chemical as the expiration date of the prepared chemical? Thanks in advance for all input. Im always amazed at how fast, how many, and how thorough/helpful the responses are! Brandi Higgins, BS, HT(ASCP) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 32 **************************************** From Stacy_McLaughlin <@t> cooley-dickinson.org Fri Jun 25 12:52:27 2010 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri Jun 25 12:52:35 2010 Subject: [Histonet] too blue Message-ID: Any ideas why patient tissue stained with Trichrome would be too blue when the controls are working perfectly? We've repeated the stain and it keeps happening. Thanks Stacy McLaughlin HT(ASCP) Lead Histology Tech./Laboratory Safety From TMcNemar <@t> lmhealth.org Fri Jun 25 13:11:10 2010 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Jun 25 13:11:17 2010 Subject: [Histonet] Full-time day Job open in Central Ohio.. Message-ID: We have and opening for a full time day shift histotech. We are a small but progressive hospital of around 250 beds in Newark, Ohio (about 30 miles from Columbus). If interested you may apply through our website (www.lmhealth.org) Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From TMcNemar <@t> lmhealth.org Fri Jun 25 13:22:01 2010 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Jun 25 13:22:08 2010 Subject: [Histonet] Prepared Solutions In-Reply-To: Message-ID: Long, long ago, we made everything up (mostly from powders), we kept all the procedures written on recipe cards filed alphabetically in a recipe box and it worked very well. About the only thing we make up anymore is formalin, and graded alcohols. I have a procedure for the formalin but none for the alcohols. We labeled the working solutions with the chemical name, percentage, and a shelf life date. Dry powders lasted forever (many did not even give an expiration) so we just fabricated a reasonable shelf life date. I also think it is advisable to list and spell out the name and concentration of every ingredient used. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Friday, June 25, 2010 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prepared Solutions Hello All, I work in a small lab where we purchase most of our chemicals already prepared. The only solutions we routinely prepare ourselves are our acid alcohol and acid water for our staining procedures, Carnoy's Fixative (since it needs to be fresh) and our 50%, 70% etc alcohol solutions for our Pap stain and for our processing machine. I would also like to start preparing our 1% and 3% acetic acid solutions instead of purchasing them since I have glacial acetic acid on hand anyway for our Carnoy's Fixative (not that the acetic acid acid solutions are expensive but every dollar counts, plus the shipping charges always shock me). My questions are 1 - in your labs, do you have a policy with instructions on how to prepare each solution? (eg 1 ml acetic acid diluted with water to 100 ml to prepate 1% Acetic Acid Aq and is it neccessary to say 500 ml water and 500 ml reagent alcohol to form 50% alcohol) 2 - when you label the solutions you prepare, do you transfer the lot numbers and expiration dates of the chemicals that were used to make the solutions onto the new chemical bottles and use the expiration date of the concentrated chemical as the expiration date of the prepared chemical? Thanks in advance for all input. Im always amazed at how fast, how many, and how thorough/helpful the responses are! Brandi Higgins, BS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From sbreeden <@t> nmda.nmsu.edu Fri Jun 25 13:29:24 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jun 25 13:29:35 2010 Subject: [Histonet] Vet Dx Labs Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E471E7@nmdamailsvr.nmda.ad.nmsu.edu> If you are the tech for one of the States' vet dx labs, could you please contact me; I have a question about the Ventana NexES and the service contract ending. I don't want to send out to the NVSL list because my list is from 2003 and I'm sure it's changed. Once I have all your names, I'll send out one email with one question about the NexES. Thanks! Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 From rjbuesa <@t> yahoo.com Fri Jun 25 14:23:14 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 25 14:23:20 2010 Subject: [Histonet] Prepared Solutions In-Reply-To: Message-ID: <388501.47415.qm@web65708.mail.ac4.yahoo.com> The preparation of your solutions should be included in your SOP. The lot numbers of your chemicals cannot be transferred to the solutions you prepare because they are a different thing. You should determine the shelf time of your working solutions and write?it in the label, along with the date they were prepared. Ren? J. --- On Fri, 6/25/10, Brandi Higgins wrote: From: Brandi Higgins Subject: [Histonet] Prepared Solutions To: histonet@lists.utsouthwestern.edu Date: Friday, June 25, 2010, 12:33 PM Hello All, I work in a small lab where we purchase most of our chemicals already prepared.? The only solutions we routinely prepare ourselves are our acid alcohol and acid water for our staining procedures, Carnoy's Fixative (since it needs to be fresh) and our 50%, 70% etc alcohol solutions for our Pap stain and for our processing machine.? I would also like to start preparing our 1% and 3% acetic acid solutions instead of purchasing them since I have glacial acetic acid on hand anyway for our Carnoy's Fixative? (not that the acetic acid acid solutions are expensive but every dollar counts, plus the shipping charges always shock me). My questions are 1 - in your labs, do you have a policy with instructions on how to prepare each solution?? (eg 1 ml acetic acid diluted with water to 100 ml to prepate 1% Acetic Acid Aq and is it neccessary to say 500 ml water and 500 ml reagent alcohol to form 50% alcohol) 2 - when you label the solutions you prepare, do you transfer the lot numbers and expiration dates of the chemicals that were used to make the solutions onto the new chemical bottles and use the expiration date of the concentrated chemical as the expiration date of the prepared chemical? Thanks in advance for all input.? Im always amazed at how fast, how many, and how thorough/helpful the responses are! Brandi Higgins, BS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nikki.Wahlberg <@t> bsci.com Fri Jun 25 14:46:11 2010 From: Nikki.Wahlberg <@t> bsci.com (Wahlberg, Nikki) Date: Fri Jun 25 14:46:18 2010 Subject: [Histonet] MMA Formulation for EXAKT Grinding Message-ID: <87224B7198E1794A8C5E679FF761CCC70CB15E423D@MAPEXCMSP01.bsci.bossci.com> Hello Everyone, I was wondering if you could help me with my MMA formulation. I have been using a formulation that I found in a published paper. My current embedding formulation is 80ml MMA, 20ml Dibutyl Phthalate and 3g Benzoyl Peroxide. The samples are embedded after three days of infiltration, one change per day, with the formulation of 80ml MMA and 20ml Dibutyl Phthalate. Lately have noticed that there is a pressure build up in the vials. I have had a few vials burst almost immediately once placed in the heated waterbath. I am filling the glass vials full with the embedding solution, capping them and then placing them in a water bath in a 37 degree oven. They are completely polymerized by the next morning. I am also getting bubbles in the plastic when polymerized. I have two questions: Is there any way to get rid of the bubbles in the plastic and of more concern what do you think is causing the pressure build up? I would really appreciate any help that you can provide. Thank you, Nikki From k84as <@t> yahoo.com Fri Jun 25 16:59:16 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Jun 25 16:59:20 2010 Subject: [Histonet] manual morphometric analysis Message-ID: <527608.24715.qm@web112613.mail.gq1.yahoo.com> hi all histonetters i need to do some morphomtric analysis on some samples of small intestin but we have no image analysis software so? i need to do it manually by occular micrometer. i need to know how to calculate villus surface area? overall mucosal surface area of cross section? ?surface area of lamina propria? mitotic figure?index?? ? i don't know how to caculate all . any help please thanks in advance ? mohamed faculty of vet. med. cairo univ.- egypt From ratliffjack <@t> hotmail.com Fri Jun 25 17:09:22 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Jun 25 17:10:31 2010 Subject: [Histonet] MMA Formulation for EXAKT Grinding In-Reply-To: <87224B7198E1794A8C5E679FF761CCC70CB15E423D@MAPEXCMSP01.bsci.bossci.com> References: <87224B7198E1794A8C5E679FF761CCC70CB15E423D@MAPEXCMSP01.bsci.bossci.com> Message-ID: Nikki, The MMA formulation you describe is one that yields a very soft block suitable for thin section (4-6 microns) microtomy. I have heard where people use this formulation for undemineralized bone and maybe even for stent work, but the softness of this formulation concerns me for the stents given the reduced stability. For me personally it is not what I use for both of these specimen types, but that is for another discussion. It is important to note that the volume of catalyst is proportional to the total volume of solution and thus this ratio (expressed as w/w) is directly proportional to the reaction product. Now to make things a little more confusing, the reaction product is influenced by air temperature, the size/density of the specimen, the total volume of reaction product, and sometimes the embedding container and void space above the solution level and container lid. Furthermore, since this reaction or polymerization of resin is an exothermic reaction, the rate (expressed as a unit of time) at which the reaction reaches the actual point at which polymerization initiates (v-max) also then influences the amount of heat that is generated from the reaction. This then is proportional to the quality of polymerization that can be seen as either a hard clear desirable block or and over polymerized, bubbled mess!!! It is my opinion that the bubbles in your specimen blocks are related to the build up of pressure in your container and caused by a rapid polymerization of your specimens by the use of the heated water bath (as per you concentration of catalyst to MMA/DBP solution) and lack of void space to buffer or diffuse excess heat. My feeling is that you are using too much catalyst in conjunction with the heat of the water bath to polymerize these specimens. Also, what is the volume of the solution you are polymerizing, how close are your specimen molds to each other in the water bath, and is the water level of the water bath at or above the embedding solution level in the specimen container? The heat generated from one specimen can sometimes add to the heat generated by another in close proximity. This then results sometimes in an over polymerization of one specimen (too much heat generated in the reaction) and no polymerization of another (absence of heat to drive the reaction). Here are my suggestions: 1) If you need specimens polymerized immediately the next day, take care to space out your specimens further apart in the water bath. Also, try turning down the water bath to reduce the secondary heat used to drive the reaction. If none of this works, then look at reducing the amount of catalyst used (may want to do this first and keep everything else the same). 2) If you can spare a few days, don't change a thing with the embedding solution, try switching your molds to polypropylene containers and leave them out on the counter at room temperature (22-23C) for 2-3 days until they polymerize. Hope this helps and it wasn't too confusing. Jack On Jun 25, 2010, at 3:46 PM, "Wahlberg, Nikki" wrote: > Hello Everyone, > > I was wondering if you could help me with my MMA formulation. I have been using a formulation that I found in a published paper. My current embedding formulation is 80ml MMA, 20ml Dibutyl Phthalate and 3g Benzoyl Peroxide. The samples are embedded after three days of infiltration, one change per day, with the formulation of 80ml MMA and 20ml Dibutyl Phthalate. Lately have noticed that there is a pressure build up in the vials. I have had a few vials burst almost immediately once placed in the heated waterbath. I am filling the glass vials full with the embedding solution, capping them and then placing them in a water bath in a 37 degree oven. They are completely polymerized by the next morning. I am also getting bubbles in the plastic when polymerized. > > I have two questions: Is there any way to get rid of the bubbles in the plastic and of more concern what do you think is causing the pressure build up? > > I would really appreciate any help that you can provide. > > Thank you, > Nikki > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lhamilton <@t> wcplaboratories.com Fri Jun 25 17:29:10 2010 From: lhamilton <@t> wcplaboratories.com (Lisa Hamilton) Date: Fri Jun 25 17:29:34 2010 Subject: [Histonet] (no subject) Message-ID: <4C252DB6.7010300@wcplaboratories.com> From lhamilton <@t> wcplaboratories.com Fri Jun 25 17:43:23 2010 From: lhamilton <@t> wcplaboratories.com (Lisa Hamilton) Date: Fri Jun 25 17:43:46 2010 Subject: [Histonet] IHC Lead Tech Position Message-ID: <4C25310B.9030902@wcplaboratories.com> Our lab is currently seeking a highly motivated histotech with a *_strong_* background in immunohistochemistry. We are located just outside St. Louis Missouri. The right candidate will take the lead role in the development and implementation of new procedures, instrumentation and quality control for the IHC department. Must have the ability to recognize probable cause of technical difficulties and to expediently remedy these difficulties. This is a full-time position, Monday-Friday. Please contact me if interested. Lisa Hamilton, HT (ASCP) Histology Supervisor (314) 991-4363 x 230 lhamilton@wcplaboratories.com From bob.nienhuis <@t> gmail.com Fri Jun 25 18:46:42 2010 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Fri Jun 25 18:46:47 2010 Subject: [Histonet] manual morphometric analysis In-Reply-To: <527608.24715.qm@web112613.mail.gq1.yahoo.com> References: <527608.24715.qm@web112613.mail.gq1.yahoo.com> Message-ID: If you have a microscope camera of some kind, ImageJ is a free dowmloadable software package that will do morphometric analysis. See: http://rsbweb.nih.gov/ij/. There is a mailing list with a lot of helpful people on it too. Bob Nienhuis UCLA / VA Medical Center Los Angeles On Fri, Jun 25, 2010 at 2:59 PM, mohamed abd el razik wrote: > hi all histonetters > i need to do some morphomtric analysis on some samples of small intestin > but we have no image analysis software so i need to do it manually by > occular micrometer. > i need to know how to calculate villus surface area? > overall mucosal surface area of cross section? > surface area of lamina propria? > mitotic figure index?? > > i don't know how to caculate all . any help please > thanks in advance > > mohamed > faculty of vet. med. > cairo univ.- egypt > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Gervaip <@t> aol.com Fri Jun 25 21:45:58 2010 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Fri Jun 25 21:47:13 2010 Subject: [Histonet] ARTICLE Message-ID: <40454.3a1f0971.3956c3e6@aol.com> I have it some where at work. I seem to remember that it was a work shop and not just an article I read. I will look for it. Pearl "Dance like no one is watching" In a message dated 6/25/2010 11:19:19 A.M. Central Daylight Time, Jackie.O'Connor@abbott.com writes: No - but I could write one. From: "Sara Baldwin/mhhcc.org" To: Date: 06/25/2010 10:53 AM Subject: [Histonet] ARTICLE Sent by: histonet-bounces@lists.utsouthwestern.edu Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called "Workplace Violence in the Laboratory" Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sj_pan <@t> yahoo.com Fri Jun 25 23:55:43 2010 From: sj_pan <@t> yahoo.com (Shirley Pan) Date: Fri Jun 25 23:55:46 2010 Subject: [Histonet] Tissue Processors Message-ID: <409923.64618.qm@web52608.mail.re2.yahoo.com> We are in the process of trying out tissue processors. Are there any users of the Leica Peloris or Thermo EG who can help us out with some opinions? Reliability, ease of changing solutions, programmability? Thanks for any help. From lpwenk <@t> sbcglobal.net Sat Jun 26 05:46:09 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Jun 26 05:46:35 2010 Subject: [Histonet] ARTICLE In-Reply-To: Message-ID: There was a teleconference on Violence in the Laboratory from NSH in 2001, given by Jan Mahoney. Is that the "article"? Peggy Wenk Beaumont Hospital Royal Oak, MI 48073 (and NSH Teleconference Coordinator) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Friday, June 25, 2010 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ARTICLE Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called "Workplace Violence in the Laboratory" Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Sat Jun 26 08:28:42 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Sat Jun 26 08:28:33 2010 Subject: [Histonet] New CAP question ANP.22760 References: <7cdd9.6a7042fa.3952c272@aol.com> <1AAF670737F193429070841C6B2ADD4C021B89F2FB@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8016114E2@EXCHANGECLUSTER.yumaregional.local> I have been reading the post to this question and it seems to me that there are different standards depending on the lab that is operating the methodology. I do agree that the core lab for years have had the instruction and training in the performance of validation. One thing that comes to mind as well is why has histology not had this training? Why are we not getting this from our certification agency, our professional societies and biggest reason where is our standardization. It seems to me that with all these regualtions in plac for so long, why were we missed. Is it because when inspected through CAP we are being inspected by a pathologist rather than a histo tech? These are some of the questions at hand. I to see new standards within the CAP checklist as well as other regulatory organizations that will affect the future of the Anatomic Pathology community. But I think we need is to provide a underlying architecture for our peers, so that we can begin the transition to the future. This is only the beginning, there is still Digital Image Analysis and Telepathology. It funny we are looking to become a hybrid of radiology and the core lab, but with the best of both worlds. Tim great structure for the validation study. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, Tim Sent: Wed 6/23/2010 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Joe, You wrote : The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time ..." Those were my thoughts exactly. While the person replying may or may not have specific histology experience she will have clinical lab experience (however, my guess is that she is exposed to histology regularly at CAP). Clinical labs have a bit of an easier time, actually, because they validate primarily to known concentration controls - analytical controls manufactured at a range of known concentrations for instance. The institution then adds in their normal controls for validation. As far as the current question about validating a new lot of reagent the best practice is to run parallel tests on the same machine. If that is not easily possible on a particular manufacturer's instrument then the question should be asked of them: Why not? If this is a requirement the manufacturer should provide an easy path to meeting the requirement. However, if that is not the case then the institution simply writes a procedure to get around the inadequacies of the instrument (Maybe the vendor can help with that). Then follow the procedure. That should satisfy inspectors. An "...appropriate panel of tissues..." is whatever the institution deems appropriate for the given antibody or reagent. This is a perfect place for tissue arrays. You can make your own or buy them. IHC must meet CLIA validation guidelines but since IHC is generally qualitative the requirements must be understood and methods adapted to a qualitative scenario. Several IHC and Histotechnology books discuss the subject at length (Taylor, Dabbs, Bancroft for instance). Below is a brief overview of how to do that. (for more in-depth info this was covered in an NSH teleconference I gave last year - PowerPoint, audio and references available from NSH-, and will be covered in a similar workshop at NSH in Seattle this year). 1) CAP General Validation CAP GEN.42020-42163 Test Method Validation Follows CLIA CFR Sec 493.1253 Does not apply well to IHC (IHC is usually qualitative) But the general principle applies: The laboratory must have data on each test's accuracy, precision, analytic sensitivity, interferences and reportable range. Unmodified FDA-cleared or approved tests: the lab may use manufacturer information or published reports but lab must verify outside data. Non-FDA cleared: Lab MUST verify or establish analytic accuracy, precision, sensitivity, specificity and reportable range. 2) Validation includes: Accuracy: Compare results with New antibody to a previously validated antibody on the same tissues Precision: Test samples with varying antigen expression Intra-run, Inter-run tests, 10 slides each (reproducibility) Sensitivity: True Positive vs False Negative (higher % FN = less sensitive) Interferences [Specificity]: True Negative vs False Positive (Higher % FP = less specific) Delineate what could interfere to give a false positive or false negative result. Reportable Range Establish a scoring system Provide the definition of a positive result 3)Sensitivity Analytic Sensitivity: Lowest amount of substance detectable by the test Can only be done with controls of known concentration Diagnostic Sensitivity: Ability of the test to determine true diagnostic positive verses false negative (higher % FN = less sensitive) Requires comparison to a previously validated antibody IHC Sensitivity: Extent to which an antibody can be diluted and still achieve target recognition. NOTE: This is determined by antibody AND detection system! 4) Specificity: Analytic Specificity Accuracy on tests of known positive and negative controls Controls of known concentration Determine what could "Interfere" to confound the result Diagnostic Specificity Ability of a test to determine true diagnostic negative verses false positives (Higher % FP = less specific) Requires comparison to a previously validated antibody IHC Specificity Ability of an antibody to bind exclusively to its particular antigen in the absence of staining of other molecules Or, staining of other structures in addition to target structures/cells (Sensitivity and Specificity adapted from: Theoretical and Practical Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote, 2005) Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMyers1@aol.com Sent: Tuesday, June 22, 2010 6:51 PM To: tjasper@copc.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Tom: As much as I agree with your acknowledgment that its seems a bit odd for the CAP to have a blood-banker responding to AP-related issue, I'm actually not surprised. The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time, and they wonder why IHC isn't expected to follow the same requirements as chemistry, immunology, etc. -- IHC is, after all, an awful lot like ELISA. And rightfully so, because IHC is, under CLIA (which supersedes CAP), considered highly-complex, non-waived testing -- and is, therefore, subject to the same Quality Systems regulations (in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. Could it be that, because AP produces qualitative results that are interpreted by a pathologist and CP produces quantitative results that are interpreted by an analyzer, we somehow think that CLIA rules don't apply to IHC? I certainly don't have the answer to that, but it make me wonder what the future holds. As witnessed by some of the newest CAP 'standards' (including the question in question...no pun intended), e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens must be tested, and where 10 of the positives must be weakly positive -- an acknowledgment that validation specimens must be carefully selected in order to obtain appropriate results), it certainly doesn't appear that the regulation of IHC testing is going to become more relaxed. Joe Myers, M.S., CT(ASCP) ------------------------------ Message: 12 Date: Fri, 18 Jun 2010 12:38:07 -0700 From: "Thomas Jasper" Subject: RE: [Histonet] New CAP question ANP.22760 To: "Mark Tarango" Cc: _histonet@lists.utsouthwestern.edu_ (mailto:histonet@lists.utsouthwestern.edu) Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From jdooley2008 <@t> yahoo.com Sat Jun 26 10:02:18 2010 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Sat Jun 26 10:02:24 2010 Subject: [Histonet] YFP staining Message-ID: <693617.99491.qm@web45904.mail.sp1.yahoo.com> I trying to do staining of cytospins from CD11c Cre x rYFP. I am getting good staining using the Rabbit anti-GFP from Rockland on tissue sections that have been fixed in 4% PFA, cryoprotected. I have fixed the cytospins with 4% PFA over night and proceeded the the same staining protocol that I used on tissue section but the staining is not working. Any advice would be greatly appreciated. Thank you, James L. Dooley VIB - Autoimmune Genetics Laboratory Campus Gasthuisberg - Department of Experimental Medicine O&N2 bus 1026 Herestraat 49 B-3000 Leuven Belgium Tel:+32 16 330583 Fax:+32 16 330591 From cdbeads <@t> earthlink.net Sat Jun 26 13:07:54 2010 From: cdbeads <@t> earthlink.net (Daniel) Date: Sat Jun 26 13:08:04 2010 Subject: [Histonet] New CAP question ANP.22760 Message-ID: <12347581.1277575674701.JavaMail.root@elwamui-cypress.atl.sa.earthlink.net> One question I think a lot of you are not considering is that the clinical laboratory usually tests for analytes present in most patients. This allows the clinical lab to more easily run validations with hundreds of patients, using statistical tools to analyze the precision, specificity and sensitivity of the test. What most of those concerned with this new CAP standard (and I count among them) is that our testing often targets a very rare population of patients. As such, an extensive validation is much more difficult to establish. It then makes the statistical models used in clinical tests much less valuble since the accuracy of these formulas decrease with smaller sample sizes. Additionally, the pathology of the clinical tests are reported as a measurement which does not have an intrinsic value. It must be interpreted by a clinician to give value to the patient. For example, the Blood Urea Nitrogen test value range usually is between 7-20 mg/dL while values above 50 are considered a marker of poor kidney function. Elevated values, however, do not mean that kidney functions are impaired but instead may be due to other pathologies such as congestive heart failure, gi bleeding, certain steroids and even diet. For histopathology even relatively common antibodies such as pan-T cell marker CD3 usually only stain 75% of T cell neoplasms. A negative result for that minority does not indicate that the tumor does not have a T cell lineage. It relies instead on the pathologist interpreting the result much as the clinician looks at the BUN value in relation to other factors in the patient's presentation. When we validate this kind of antibody, do we perform testing then on T cells and B cells (give me a good tonsil), on malignant samples or some combination of the two? What is a reasonable number of tests to run then? If I choose 10 normal lymphatic tissue blocks for my routine T/B cell marking (it can't be as extensive as ER/PR can it?) how many samples should I choose for my malignant population? If I use another 10 anyone with a background in statistics will tell you that the sample size is too small to interpret accuracy. If I push it up to 20, 50 or 100 I would be certain that many laboratories would not be able to afford the cost of the validation. This would then push many good laboratories out of the business of IHC with the unintended result of delaying diagnoses and increasing patient costs by driving testing to fewer and fewer testing outlets. CAP's new path with ER/PR seems to be trying to achieve a noble end of improving quality in the laboratory but without an understanding of the complexities and consequences of the method they are implementing. Dan -----Original Message----- >From: Jesus Ellin >Sent: Jun 26, 2010 6:28 AM >To: "Morken, Tim" , histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >I have been reading the post to this question and it seems to me that there are different standards depending on the lab that is operating the methodology. I do agree that the core lab for years have had the instruction and training in the performance of validation. One thing that comes to mind as well is why has histology not had this training? Why are we not getting this from our certification agency, our professional societies and biggest reason where is our standardization. It seems to me that with all these regualtions in plac for so long, why were we missed. Is it because when inspected through CAP we are being inspected by a pathologist rather than a histo tech? These are some of the questions at hand. I to see new standards within the CAP checklist as well as other regulatory organizations that will affect the future of the Anatomic Pathology community. But I think we need is to provide a underlying architecture for our peers, so that we can begin the transition to the future. This is only the beginning, there is still Digital Image Analysis and Telepathology. It funny we are looking to become a hybrid of radiology and the core lab, but with the best of both worlds. Tim great structure for the validation study. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, Tim >Sent: Wed 6/23/2010 9:48 AM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Joe, > >You wrote : The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time ..." > >Those were my thoughts exactly. While the person replying may or may not have specific histology experience she will have clinical lab experience (however, my guess is that she is exposed to histology regularly at CAP). Clinical labs have a bit of an easier time, actually, because they validate primarily to known concentration controls - analytical controls manufactured at a range of known concentrations for instance. The institution then adds in their normal controls for validation. > >As far as the current question about validating a new lot of reagent the best practice is to run parallel tests on the same machine. If that is not easily possible on a particular manufacturer's instrument then the question should be asked of them: Why not? If this is a requirement the manufacturer should provide an easy path to meeting the requirement. However, if that is not the case then the institution simply writes a procedure to get around the inadequacies of the instrument (Maybe the vendor can help with that). Then follow the procedure. That should satisfy inspectors. > >An "...appropriate panel of tissues..." is whatever the institution deems appropriate for the given antibody or reagent. This is a perfect place for tissue arrays. You can make your own or buy them. > >IHC must meet CLIA validation guidelines but since IHC is generally qualitative the requirements must be understood and methods adapted to a qualitative scenario. Several IHC and Histotechnology books discuss the subject at length (Taylor, Dabbs, Bancroft for instance). > >Below is a brief overview of how to do that. (for more in-depth info this was covered in an NSH teleconference I gave last year - PowerPoint, audio and references available from NSH-, and will be covered in a similar workshop at NSH in Seattle this year). > > >1) >CAP General Validation >CAP GEN.42020-42163 Test Method Validation Follows CLIA CFR Sec 493.1253 Does not apply well to IHC (IHC is usually qualitative) > >But the general principle applies: >The laboratory must have data on each test's accuracy, precision, analytic sensitivity, interferences and reportable range. > >Unmodified FDA-cleared or approved tests: the lab may use manufacturer information or published reports but lab must verify outside data. > >Non-FDA cleared: Lab MUST verify or establish analytic accuracy, precision, sensitivity, specificity and reportable range. > >2) Validation includes: >Accuracy: > Compare results with New antibody to a previously validated antibody on the same tissues > >Precision: > Test samples with varying antigen expression > Intra-run, Inter-run tests, 10 slides each (reproducibility) > >Sensitivity: > True Positive vs False Negative (higher % FN = less sensitive) > >Interferences [Specificity]: > True Negative vs False Positive (Higher % FP = less specific) > Delineate what could interfere to give a false positive or false negative result. > >Reportable Range > Establish a scoring system > Provide the definition of a positive result > >3)Sensitivity > >Analytic Sensitivity: > Lowest amount of substance detectable by the test > Can only be done with controls of known concentration > >Diagnostic Sensitivity: > Ability of the test to determine true diagnostic positive verses false negative (higher % FN = less sensitive) > Requires comparison to a previously validated antibody > >IHC Sensitivity: > Extent to which an antibody can be diluted and still achieve target recognition. NOTE: This is determined by antibody AND detection system! > > > >4) Specificity: > >Analytic Specificity > Accuracy on tests of known positive and negative controls > Controls of known concentration > Determine what could "Interfere" to confound the result > > >Diagnostic Specificity > Ability of a test to determine true diagnostic negative verses false positives (Higher % FP = less specific) > Requires comparison to a previously validated antibody > > >IHC Specificity > Ability of an antibody to bind exclusively to its particular antigen in the absence of staining of other molecules > Or, staining of other structures in addition to target structures/cells > >(Sensitivity and Specificity adapted from: Theoretical and Practical Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote, 2005) > >Tim Morken >Supervisor, Histology / IPOX >UCSF Medical Center >San Francisco, CA > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMyers1@aol.com >Sent: Tuesday, June 22, 2010 6:51 PM >To: tjasper@copc.net >Cc: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Tom: > >As much as I agree with your acknowledgment that its seems a bit odd for >the CAP to have a blood-banker responding to AP-related issue, I'm actually >not surprised. The folks in the 'clinical' lab have been performing more >comprehensive and complex validation procedures for a very long time, and they >wonder why IHC isn't expected to follow the same requirements as chemistry, >immunology, etc. -- IHC is, after all, an awful lot like ELISA. And >rightfully so, because IHC is, under CLIA (which supersedes CAP), considered >highly-complex, non-waived testing -- and is, therefore, subject to the same >Quality Systems regulations (in particular, 42CFR493.1252-1256, 1273, and 1281) as >the testing performed in other areas of the lab. > >Could it be that, because AP produces qualitative results that are >interpreted by a pathologist and CP produces quantitative results that are >interpreted by an analyzer, we somehow think that CLIA rules don't apply to IHC? I >certainly don't have the answer to that, but it make me wonder what the >future holds. As witnessed by some of the newest CAP 'standards' (including the >question in question...no pun intended), e.g. ER/PR, where a minimum of 20 >positive and 20 negative specimens must be tested, and where 10 of the >positives must be weakly positive -- an acknowledgment that validation specimens >must be carefully selected in order to obtain appropriate results), it >certainly doesn't appear that the regulation of IHC testing is going to become >more relaxed. > >Joe Myers, M.S., CT(ASCP) > >------------------------------ > >Message: 12 >Date: Fri, 18 Jun 2010 12:38:07 -0700 >From: "Thomas Jasper" >Subject: RE: [Histonet] New CAP question ANP.22760 >To: "Mark Tarango" >Cc: _histonet@lists.utsouthwestern.edu_ >(mailto:histonet@lists.utsouthwestern.edu) > >Mark, > >Did you notice the credentials from this CAP representative? MT with a >Blood Bank specialty I believe. What I glean from that is...more than >likely this person does not grasp the logistics of "contemporaneously" >staining identical Abs from separate lots. She also likely does not >understand the logistical application for detection and automation >either. > >I'm not trying to be overly critical of this person. I'm sure she is >quite intelligent and would not have the MT/SBB if she wasn't >intelligent. It comes down to a lack of understanding Anatomic >Pathology testing application re: automated IHC. I believe this is a >common problem in and out of CAP. Many lab directors and other folks in >positions of authority without AP/Histology/Cytology backgrounds seem to >believe that broad clinical lab modalities apply to Anatomic Path >scenarios. I used to refer to this in my former position as - "Trying >to put the yoke of clinical lab onto anatomic path." We are >laboratorians, but in many instances do not fit the general clinical lab >mold. > >It's unfortunate that CAP has put this person in the position to >respond. It is apparent to me that she's not grasping the particulars >here. She probably never will unless she decides to go into a working, >automated IHC "tissue" lab and take the time to ask questions and >understand (learn) what we're all about. > >Thanks, >Tom Jasper > >Thomas Jasper HT (ASCP) BAS >Histology Supervisor >Central Oregon Regional Pathology Services >Bend, OR 97701 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >______________________________________________________________________ >This message is confidential, intended only for the named >recipient(s) and may contain information that is privileged >or exempt from disclosure under applicable law. If you are >not the intended recipient(s), you are notified that the >dissemination, distribution, or copying of this message is >strictly prohibited. If you receive this message in error, >or are not the named recipient(s), please notify the sender >at either the e-mail, fax, address, or telephone number >listed above and delete this e-mail from your computer. >Thank You. >______________________________________________________________________ >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From k84as <@t> yahoo.com Sat Jun 26 14:40:50 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sat Jun 26 14:40:53 2010 Subject: Fw: [Histonet] Tissue Processors Message-ID: <748458.69728.qm@web112603.mail.gq1.yahoo.com> i need it too as we are going to bring new tissue processor to our small lab --- On Sat, 6/26/10, Shirley Pan wrote: From: Shirley Pan Subject: [Histonet] Tissue Processors To: histonet@lists.utsouthwestern.edu Date: Saturday, June 26, 2010, 7:55 AM We are in the process of trying out tissue processors. Are there any users of the Leica Peloris or Thermo EG who can help us out with some opinions? Reliability, ease of changing solutions, programmability? Thanks for any help. ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Sat Jun 26 15:45:19 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Sat Jun 26 15:45:26 2010 Subject: [Histonet] New CAP question ANP.22760 References: <12347581.1277575674701.JavaMail.root@elwamui-cypress.atl.sa.earthlink.net> Message-ID: <90354A475B420441B2A0396E5008D49692BF70@copc-sbs.COPC.local> Daniel, I think your observations are very good. Which brings me again to a point I was trying to make earlier. Anatomic Pathology is not the same as the General Clinical Lab or it's close counterparts. It almost seems like this is overblown re: validation. As you've pointed out, the interpretation is up to the pathologist. Patients (prostate, i.e.) will correlate with other tests. When known positives demonstrate properly you're valid. Even automated systems can use algorithms to score strength of positivity...which varies yet will show as positive (valid). You receive a new batch of Ab you run it on a known positive, that's the bottom line. It seems somehow CAP or clinical lab (trained) folks want more than that. And again (as you pointed out) the appreciation for the differences in how we operate as laboratorians seems to fall by the wayside. As you mention pushing 20, 50, 100 is not feasible (and frankly unnecessary). I don't know where the answer lies in making everyone happy here. I do know that some thinking outside the box and trying understand AP is required. We all want good patient care, but one size does not fit all. Thanks, tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Sent: Saturday, June 26, 2010 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 One question I think a lot of you are not considering is that the clinical laboratory usually tests for analytes present in most patients. This allows the clinical lab to more easily run validations with hundreds of patients, using statistical tools to analyze the precision, specificity and sensitivity of the test. What most of those concerned with this new CAP standard (and I count among them) is that our testing often targets a very rare population of patients. As such, an extensive validation is much more difficult to establish. It then makes the statistical models used in clinical tests much less valuble since the accuracy of these formulas decrease with smaller sample sizes. Additionally, the pathology of the clinical tests are reported as a measurement which does not have an intrinsic value. It must be interpreted by a clinician to give value to the patient. For example, the Blood Urea Nitrogen test value range usually is between 7-20 mg/dL while values above 50 are considered a marker of poor kidney function. Elevated values, however, do not mean that kidney functions are impaired but instead may be due to other pathologies such as congestive heart failure, gi bleeding, certain steroids and even diet. For histopathology even relatively common antibodies such as pan-T cell marker CD3 usually only stain 75% of T cell neoplasms. A negative result for that minority does not indicate that the tumor does not have a T cell lineage. It relies instead on the pathologist interpreting the result much as the clinician looks at the BUN value in relation to other factors in the patient's presentation. When we validate this kind of antibody, do we perform testing then on T cells and B cells (give me a good tonsil), on malignant samples or some combination of the two? What is a reasonable number of tests to run then? If I choose 10 normal lymphatic tissue blocks for my routine T/B cell marking (it can't be as extensive as ER/PR can it?) how many samples should I choose for my malignant population? If I use another 10 anyone with a background in statistics will tell you that the sample size is too small to interpret accuracy. If I push it up to 20, 50 or 100 I would be certain that many laboratories would not be able to afford the cost of the validation. This would then push many good laboratories out of the business of IHC with the unintended result of delaying diagnoses and increasing patient costs by driving testing to fewer and fewer testing outlets. CAP's new path with ER/PR seems to be trying to achieve a noble end of improving quality in the laboratory but without an understanding of the complexities and consequences of the method they are implementing. Dan -----Original Message----- >From: Jesus Ellin >Sent: Jun 26, 2010 6:28 AM >To: "Morken, Tim" , >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >I have been reading the post to this question and it seems to me that there are different standards depending on the lab that is operating the methodology. I do agree that the core lab for years have had the instruction and training in the performance of validation. One thing that comes to mind as well is why has histology not had this training? Why are we not getting this from our certification agency, our professional societies and biggest reason where is our standardization. It seems to me that with all these regualtions in plac for so long, why were we missed. Is it because when inspected through CAP we are being inspected by a pathologist rather than a histo tech? These are some of the questions at hand. I to see new standards within the CAP checklist as well as other regulatory organizations that will affect the future of the Anatomic Pathology community. But I think we need is to provide a underlying architecture for our peers, so that we can begin the transition to the future. This is only the beginning, there is still Digital Image Analysis and Telepathology. It funny we are looking to become a hybrid of radiology and the core lab, but with the best of both worlds. Tim great structure for the validation study. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, >Tim >Sent: Wed 6/23/2010 9:48 AM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Joe, > >You wrote : The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time ..." > >Those were my thoughts exactly. While the person replying may or may not have specific histology experience she will have clinical lab experience (however, my guess is that she is exposed to histology regularly at CAP). Clinical labs have a bit of an easier time, actually, because they validate primarily to known concentration controls - analytical controls manufactured at a range of known concentrations for instance. The institution then adds in their normal controls for validation. > >As far as the current question about validating a new lot of reagent the best practice is to run parallel tests on the same machine. If that is not easily possible on a particular manufacturer's instrument then the question should be asked of them: Why not? If this is a requirement the manufacturer should provide an easy path to meeting the requirement. However, if that is not the case then the institution simply writes a procedure to get around the inadequacies of the instrument (Maybe the vendor can help with that). Then follow the procedure. That should satisfy inspectors. > >An "...appropriate panel of tissues..." is whatever the institution deems appropriate for the given antibody or reagent. This is a perfect place for tissue arrays. You can make your own or buy them. > >IHC must meet CLIA validation guidelines but since IHC is generally qualitative the requirements must be understood and methods adapted to a qualitative scenario. Several IHC and Histotechnology books discuss the subject at length (Taylor, Dabbs, Bancroft for instance). > >Below is a brief overview of how to do that. (for more in-depth info this was covered in an NSH teleconference I gave last year - PowerPoint, audio and references available from NSH-, and will be covered in a similar workshop at NSH in Seattle this year). > > >1) >CAP General Validation >CAP GEN.42020-42163 Test Method Validation Follows CLIA CFR Sec >493.1253 Does not apply well to IHC (IHC is usually qualitative) > >But the general principle applies: >The laboratory must have data on each test's accuracy, precision, analytic sensitivity, interferences and reportable range. > >Unmodified FDA-cleared or approved tests: the lab may use manufacturer information or published reports but lab must verify outside data. > >Non-FDA cleared: Lab MUST verify or establish analytic accuracy, precision, sensitivity, specificity and reportable range. > >2) Validation includes: >Accuracy: > Compare results with New antibody to a previously validated antibody on the same tissues > >Precision: > Test samples with varying antigen expression > Intra-run, Inter-run tests, 10 slides each (reproducibility) > >Sensitivity: > True Positive vs False Negative (higher % FN = less sensitive) > >Interferences [Specificity]: > True Negative vs False Positive (Higher % FP = less specific) > Delineate what could interfere to give a false positive or false negative result. > >Reportable Range > Establish a scoring system > Provide the definition of a positive result > >3)Sensitivity > >Analytic Sensitivity: > Lowest amount of substance detectable by the test > Can only be done with controls of known concentration > >Diagnostic Sensitivity: > Ability of the test to determine true diagnostic positive verses false negative (higher % FN = less sensitive) > Requires comparison to a previously validated antibody > >IHC Sensitivity: > Extent to which an antibody can be diluted and still achieve target recognition. NOTE: This is determined by antibody AND detection system! > > > >4) Specificity: > >Analytic Specificity > Accuracy on tests of known positive and negative controls > Controls of known concentration > Determine what could "Interfere" to confound the result > > >Diagnostic Specificity > Ability of a test to determine true diagnostic negative verses false positives (Higher % FP = less specific) > Requires comparison to a previously validated antibody > > >IHC Specificity > Ability of an antibody to bind exclusively to its particular antigen in the absence of staining of other molecules > Or, staining of other structures in addition to target >structures/cells > >(Sensitivity and Specificity adapted from: Theoretical and Practical >Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote, 2005) > >Tim Morken >Supervisor, Histology / IPOX >UCSF Medical Center >San Francisco, CA > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >JMyers1@aol.com >Sent: Tuesday, June 22, 2010 6:51 PM >To: tjasper@copc.net >Cc: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Tom: > >As much as I agree with your acknowledgment that its seems a bit odd >for the CAP to have a blood-banker responding to AP-related issue, I'm >actually not surprised. The folks in the 'clinical' lab have been >performing more comprehensive and complex validation procedures for a >very long time, and they wonder why IHC isn't expected to follow the >same requirements as chemistry, immunology, etc. -- IHC is, after all, >an awful lot like ELISA. And rightfully so, because IHC is, under CLIA >(which supersedes CAP), considered highly-complex, non-waived testing >-- and is, therefore, subject to the same Quality Systems regulations >(in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. > >Could it be that, because AP produces qualitative results that are >interpreted by a pathologist and CP produces quantitative results that >are interpreted by an analyzer, we somehow think that CLIA rules don't >apply to IHC? I certainly don't have the answer to that, but it make >me wonder what the future holds. As witnessed by some of the newest >CAP 'standards' (including the question in question...no pun intended), >e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens >must be tested, and where 10 of the positives must be weakly positive >-- an acknowledgment that validation specimens must be carefully >selected in order to obtain appropriate results), it certainly doesn't >appear that the regulation of IHC testing is going to become more relaxed. > >Joe Myers, M.S., CT(ASCP) > >------------------------------ > >Message: 12 >Date: Fri, 18 Jun 2010 12:38:07 -0700 >From: "Thomas Jasper" >Subject: RE: [Histonet] New CAP question ANP.22760 >To: "Mark Tarango" >Cc: _histonet@lists.utsouthwestern.edu_ >(mailto:histonet@lists.utsouthwestern.edu) > >Mark, > >Did you notice the credentials from this CAP representative? MT with a >Blood Bank specialty I believe. What I glean from that is...more than >likely this person does not grasp the logistics of "contemporaneously" >staining identical Abs from separate lots. She also likely does not >understand the logistical application for detection and automation >either. > >I'm not trying to be overly critical of this person. I'm sure she is >quite intelligent and would not have the MT/SBB if she wasn't >intelligent. It comes down to a lack of understanding Anatomic >Pathology testing application re: automated IHC. I believe this is a >common problem in and out of CAP. Many lab directors and other folks in >positions of authority without AP/Histology/Cytology backgrounds seem >to believe that broad clinical lab modalities apply to Anatomic Path >scenarios. I used to refer to this in my former position as - "Trying >to put the yoke of clinical lab onto anatomic path." We are >laboratorians, but in many instances do not fit the general clinical >lab mold. > >It's unfortunate that CAP has put this person in the position to >respond. It is apparent to me that she's not grasping the particulars >here. She probably never will unless she decides to go into a working, >automated IHC "tissue" lab and take the time to ask questions and >understand (learn) what we're all about. > >Thanks, >Tom Jasper > >Thomas Jasper HT (ASCP) BAS >Histology Supervisor >Central Oregon Regional Pathology Services Bend, OR 97701 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >______________________________________________________________________ >This message is confidential, intended only for the named >recipient(s) and may contain information that is privileged or exempt >from disclosure under applicable law. If you are not the intended >recipient(s), you are notified that the dissemination, distribution, or >copying of this message is strictly prohibited. If you receive this >message in error, or are not the named recipient(s), please notify the >sender at either the e-mail, fax, address, or telephone number listed >above and delete this e-mail from your computer. >Thank You. >______________________________________________________________________ >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From k84as <@t> yahoo.com Sat Jun 26 17:39:19 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sat Jun 26 17:39:23 2010 Subject: [Histonet] what do you think? Message-ID: <602647.18150.qm@web112609.mail.gq1.yahoo.com> dear histonetters i have a quistion please regarding the thickness of lamina propria in small or larg intestine. what is the significant of it?? does it mean inflamation and pathological condition or mean more size and so absorption of nutrients? i mean does the thickning is favorable or not? ? thanks Mohamed Abd Elrazik Histology dep. Fac. of Vet. Med. Cairo . Univ. -Egypt From malbenatti <@t> gmail.com Sun Jun 27 05:38:17 2010 From: malbenatti <@t> gmail.com (Malika Benatti) Date: Sun Jun 27 05:47:58 2010 Subject: [Histonet] H1B Visa Sponsorship Message-ID: <6384F5A6-1B53-4E16-8B72-7DE8375C36B5@gmail.com> To all Histonet list members in Knoxville Area I am Specialist Biomedical Scientist fully trained in the United Kingdom with a BSc Hons in Biomedical Science and a Post Graduate Certificate in Cellular Pathology from the University of Westminster in London United Kingdom and currently looking for a H1B Sponsor to work as a histotechnologist, as I currently looking to relocate in the United States in Knoxville Area. I am fully registered with the Health Professional council in the UK (Similar to the US, ASCP Certification), with 8 years Post HPC Registration. I am fully trained in all the aspect of routine histology technical work and I have a very strong background in pediatric pathology, and 9 years experience in general pathology. including cut-up, special stains, immunocytochemistry, urgent intra operative frozen sections, neuropathology, muscle biopsy, and electron microscopy tissue processing. Malika From amosbrooks <@t> gmail.com Sun Jun 27 09:35:40 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Jun 27 09:35:45 2010 Subject: [Histonet] too blue Message-ID: Hi Stacy, Trichrome stains are REALLY sensitive to fixation variations. I have a great image of two heart samples stained simultaneously. Both were formalin fixed, but one was fixed overnight and the other (if you can call it fixed) was dropped into a full container and probably saw a half hour fixation total, and since it was on top of a pile of cassettes probably never really was immersed. The properly fixed specimen has red muscle and blue collagen as is expected in heart tissue. The other cut miserably, has folds & wrinkles, and the muscle is mostly light blue with darker blue collagen. Red blood cells are red, and some blushes of muscle fibers have taken on some faint red, but the difference is striking. Fixation is probably more critical to proper staining than the actual time in staining solutions. That is where I would start looking. Good luck, Amos Message: 3 Date: Fri, 25 Jun 2010 13:52:27 -0400 From: "Stacy McLaughlin" Subject: [Histonet] too blue To: Message-ID: < CB8866A9D4ECB049968DB571CF4863A3060DE0A7@cdhmail01.cooley-dickinson.org> Content-Type: text/plain; charset="iso-8859-1" Any ideas why patient tissue stained with Trichrome would be too blue when the controls are working perfectly? We've repeated the stain and it keeps happening. Thanks Stacy McLaughlin HT(ASCP) Lead Histology Tech./Laboratory Safety From laura_conner <@t> hotmail.com Sun Jun 27 12:11:36 2010 From: laura_conner <@t> hotmail.com (Laura Conner) Date: Sun Jun 27 12:11:49 2010 Subject: [Histonet] IHC for cells grown on cover slips Message-ID: Hi, I've never done IHC before and am trying to do IHC with RPE cells grown on coverslips and using a cytokeratin antibody - visualizing with peroxidase. I didn't have any luck my first time and so was wondering if anybody has some good general resources to read, or any tips that may be second nature to most but not to me since I'm a complete newbie. Any protocols? Any help would be appreciated. Thanks so much! Laura _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4 From Maxim_71 <@t> mail.ru Sun Jun 27 13:46:40 2010 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sun Jun 27 13:45:12 2010 Subject: [Histonet] CAP questionnaire for AP and IHC Message-ID: <596502057.20100627224640@mail.ru> Dear Histonetters! Can anyone send for me the last full pdf-version on CAP questionnaire for AP and IHC lab, please? I can not able to download it from CAP website. Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From a.thotakura <@t> imperial.ac.uk Mon Jun 28 07:11:20 2010 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Mon Jun 28 07:11:40 2010 Subject: [Histonet] Immunofloresence Message-ID: Dear All, I have a problem with Immunoflorescence.(IF) I made a monoclonal antibody, which works fine for western blot. But when I was doing IF I had a problem. I can see the staining in my KO and WT mice, I don't expect to see any staining in the KO mice. This same monoclonal antibody works fine for western blot, I don't see any band in KO cell lysates. Can you guys please help me in this. Does antigen retrival help? Thank you very much in advance. Many Thanks, Anil Kumar. From aajl7979 <@t> hotmail.com Mon Jun 28 08:19:42 2010 From: aajl7979 <@t> hotmail.com (Amanda L) Date: Mon Jun 28 08:19:46 2010 Subject: [Histonet] Microscope parts Message-ID: Good Morning, I have been asked to order a new eye piece and a blue filter for one of our microscopes. I checked the company that makes the scope and couldn't really find what I was looking for. Any Suggestions? Thanks in Advance Amanda _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From mtighe <@t> trudeauinstitute.org Mon Jun 28 08:03:16 2010 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Mon Jun 28 08:46:21 2010 Subject: [Histonet] Granzyme B and Lamp1 (CD107A) Message-ID: <4C286473.26E4.00EE.0@trudeauinstitute.org> Hey! Has anyone looked at Granzyme B and/or Lamp1(CD107A) in mouse cells by immunofluorescence? I know this is done for flow cytometry but thought someone may have a good method for cytospun cells. Thanks for any ideas!!! Mike From jbirkner <@t> colabserv.com Mon Jun 28 08:59:11 2010 From: jbirkner <@t> colabserv.com (Jeff Birkner) Date: Mon Jun 28 08:59:16 2010 Subject: [Histonet] workload productivity Message-ID: Does anyone have a spreadsheet they can share regarding workload/productivity in histology? Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. From sbreeden <@t> nmda.nmsu.edu Mon Jun 28 10:00:34 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Jun 28 10:00:40 2010 Subject: [Histonet] State Veterinary Diagnostic Labs Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47200@nmdamailsvr.nmda.ad.nmsu.edu> Are there any other State vet dx labs besides Minnesota, Kansas, Ohio and Illinois that would like to participate in my Poll about Ventana service contracts? I need more input! Thanks! Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 From rjbuesa <@t> yahoo.com Mon Jun 28 10:30:09 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 28 10:30:20 2010 Subject: [Histonet] Microscope parts In-Reply-To: Message-ID: <372532.48831.qm@web65707.mail.ac4.yahoo.com> Check in eBay. They usually have microscope parts for sale. Ren? J. --- On Mon, 6/28/10, Amanda L wrote: From: Amanda L Subject: [Histonet] Microscope parts To: "histonet" Date: Monday, June 28, 2010, 9:19 AM Good Morning, I have been asked to order a new eye piece and a blue filter for one of our microscopes.? I checked the company that makes the scope and couldn't really find what I was looking for.? Any Suggestions? Thanks in Advance Amanda ??? ???????? ?????? ??? ? _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Mon Jun 28 10:25:38 2010 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Jun 28 10:41:33 2010 Subject: [Histonet] Joe Myers Message-ID: <4C2886B3.2B7F.00C9.0@geisinger.edu> Joe, if you're out there, will you give me a call or respond offline to my email ?? My number is 570-214-9634 Thanks, Angie Bitting Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From TJJ <@t> stowers.org Mon Jun 28 10:49:02 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Jun 28 10:49:10 2010 Subject: [Histonet] Re: YFP staining Message-ID: Hi James, Have you checked under the microscope at your cytospins to verify there is YFP in the sample? That would be the first thing I looked at. Next, are you positive the anti-GFP antibody is cross-reacting with the YFP in your tissue samples? Do you have any non-YFP tissue samples that you have stained with the antibody? If you're sure the antibody is working properly, and there is YFP in your sample, then you should look at your fixation. I think you're overfixing the cells. A cell monolayer doesn't require very long fixation. There's no connective tissue and layers upon layers of cells to penetrate and fix. I would cut that down to 1 hour, or even better do a time course using whatever timeline you think is reasonable and see if fixation plays a role in your staining. Start there and see if that helps. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From Rick.Garnhart <@t> memorialhealthsystem.com Mon Jun 28 10:54:53 2010 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Mon Jun 28 10:55:03 2010 Subject: [Histonet] Unstained Slides In-Reply-To: Message-ID: Histoland, How is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From kdboydhisto <@t> yahoo.com Mon Jun 28 11:53:52 2010 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Mon Jun 28 11:53:56 2010 Subject: [Histonet] Tissue Processors Message-ID: <138519.24896.qm@web58606.mail.re3.yahoo.com> We have been using our Leica Peloris since January and I really, really?like it.?The processing is great even?at two hours. It is very user friendly and so easy to change out the reagents. It has saved us tons of time.?They have excellent service too. Feel free to contact me?with any specific questions. ? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services Tele (252)-830-6866 ????????(800)-284-0672 Cell? (252)-943-9527 Fax? (252)-830-0032 From Rcartun <@t> harthosp.org Mon Jun 28 11:54:46 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Jun 28 11:54:57 2010 Subject: [Histonet] Unstained Slides In-Reply-To: References: Message-ID: <4C289B95.7400.0077.1@harthosp.org> We bake our unstains at 60 degrees C. for 30 minutes prior to filing at RT. There is no set rule for stability. It all depends on fixation, the nature of the protein target, and storage conditions. I've seen absolutely spectacular immunoreactivity on unstained slides stored at RT for 20 years (and longer) and I've seen reduced immunoreactivity in unstained slides stored for as little as 4 weeks. We will always attempt to stain unstained slides when available; however, if the lesion or tumor is negative, and there is no internal control, you better cut fresh sections. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 6/28/2010 11:54 AM >>> Histoland, How is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Jun 28 12:01:30 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Jun 28 12:01:56 2010 Subject: [Histonet] Unstained Slides In-Reply-To: References: , Message-ID: We store them in the refrigerator. And depending on the antibody they are probably good for 6 months. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rick.Garnhart@memorialhealthsystem.com [Rick.Garnhart@memorialhealthsystem.com] Sent: Monday, June 28, 2010 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unstained Slides Histoland, How is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JKELL2 <@t> capefearvalley.com Mon Jun 28 13:03:22 2010 From: JKELL2 <@t> capefearvalley.com (Jason Keller) Date: Mon Jun 28 13:03:46 2010 Subject: [Histonet] Special Stainer Message-ID: <7D41E97C787DF44996E0055C8170366301263553@ntexchange3.capefear.local> I am looking to replace the automated stainer for special stains in our lab. I am currently collecting quotes but would also appreciate some input regarding satisfaction or dissatisfaction with some of the models that are available. Any input on what you think of some of these machines/companies would be appreciated. Thank you! CONFIDENTIALITY NOTICE: This electronic mail transmission may contain information that is privileged and/or confidential. Additionally, this communication may contain individual protected health information ("PHI") that is subject to protection under state and federal laws, or other privileged, confidential or proprietary information of Cape Fear Valley Health System that may not be further disclosed. Please be advised that any disclosure, copying, distribution or other use of the contents of this message by anyone other than the intended recipient is prohibited. If you have received this communication in error, please notify the sender immediately by replying to the message and deleting it from your computer. From millicentbruce <@t> gmail.com Mon Jun 28 13:07:48 2010 From: millicentbruce <@t> gmail.com (Millicent Haydel) Date: Mon Jun 28 13:08:19 2010 Subject: [Histonet] Fwd: elastic fiber / solar elastosis staining black References: Message-ID: <46CB0B2B-E3B2-43C7-86D6-E0866C63F8F8@gmail.com> > > I work in a private office where we do frozen H&E's. Today and the > end of last week we have been having trouble with our stain. The > elastic fibers / solar elastosis have been staining black. Normally > they are blue. I have tried changing the stain, and changing our > bottles of OCT and mounting medium. We are still having the same > problem. Can anyone please help. I have sent in a picture to www.histonet.org > with the title "elastic fibers staining black". From JWeems <@t> sjha.org Mon Jun 28 13:28:48 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Jun 28 13:28:51 2010 Subject: [Histonet] Coverslips Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C75@CHEXCMS10.one.ads.che.org> For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sfeher <@t> CMC-NH.ORG Mon Jun 28 13:55:47 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Jun 28 13:55:53 2010 Subject: [Histonet] Tissue Processors In-Reply-To: <748458.69728.qm@web112603.mail.gq1.yahoo.com> References: <748458.69728.qm@web112603.mail.gq1.yahoo.com> Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B75511@exchange.cmc-nh.org> We have 2 Peloris processors and run protocols ranging from 2 hrs to overnight. Several 2 and 4 hour protocols daily keeps a constant flow of specimens moving through the lab for those interested in LEAN small batch processing. As far as programmability, it 's all touch screen driven and easy. It's a smart processor so we don't change all solutions weekly. We only change the ones that are needed. The parameters that determine how long to use a particular solution are also programmable. Changing solutions is done by pumping out of the individual bottles and into waste containers and refilling is pumped from clean containers of solution back into the original containers. No heavy lifting required. Ours are in a separate room from our microtomy area so we hooked a simple door bell up to the local alarm jack on the back. When processing is done, we get the appropriate tone. Remote alarm is also tied in to the hospital switchboard in case a processor goes down at night or during the weekend. We really like the versatility and dependability of these processors. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Saturday, June 26, 2010 3:41 PM To: Histonet@lists.utsouthwestern.edu Subject: Fw: [Histonet] Tissue Processors i need it too as we are going to bring new tissue processor to our small lab --- On Sat, 6/26/10, Shirley Pan wrote: From: Shirley Pan Subject: [Histonet] Tissue Processors To: histonet@lists.utsouthwestern.edu Date: Saturday, June 26, 2010, 7:55 AM We are in the process of trying out tissue processors. Are there any users of the Leica Peloris or Thermo EG who can help us out with some opinions? Reliability, ease of changing solutions, programmability? Thanks for any help. ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Mon Jun 28 13:59:13 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Jun 28 13:59:23 2010 Subject: [Histonet] Special Stainer In-Reply-To: <7D41E97C787DF44996E0055C8170366301263553@ntexchange3.capefear.local> References: <7D41E97C787DF44996E0055C8170366301263553@ntexchange3.capefear.local> Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B75512@exchange.cmc-nh.org> Dako's Artisan Link is great. We are looking to interface this with our SoftPath LIS system. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason Keller Sent: Monday, June 28, 2010 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainer I am looking to replace the automated stainer for special stains in our lab. I am currently collecting quotes but would also appreciate some input regarding satisfaction or dissatisfaction with some of the models that are available. Any input on what you think of some of these machines/companies would be appreciated. Thank you! CONFIDENTIALITY NOTICE: This electronic mail transmission may contain information that is privileged and/or confidential. Additionally, this communication may contain individual protected health information ("PHI") that is subject to protection under state and federal laws, or other privileged, confidential or proprietary information of Cape Fear Valley Health System that may not be further disclosed. Please be advised that any disclosure, copying, distribution or other use of the contents of this message by anyone other than the intended recipient is prohibited. If you have received this communication in error, please notify the sender immediately by replying to the message and deleting it from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Mon Jun 28 14:06:08 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Jun 28 14:06:10 2010 Subject: [Histonet] RE: Coverslips In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C75@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C75@CHEXCMS10.one.ads.che.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C93@CHEXCMS10.one.ads.che.org> I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From BoozerKA <@t> ah.org Mon Jun 28 14:21:33 2010 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Mon Jun 28 14:22:00 2010 Subject: [Histonet] Coverslips In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C75@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C75@CHEXCMS10.one.ads.che.org> Message-ID: <4C2893CC.4AA8.00C0.1@ah.org> I am having the same issues and we purchased the expensive "Platinum" to avoid that problem. They just sent me replacements and I haven't tested them all yet. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org >>> "Weems, Joyce" 6/28/2010 11:28 AM >>> For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Mon Jun 28 14:36:22 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Jun 28 14:36:42 2010 Subject: [Histonet] RE: Coverslips In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C93@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C75@CHEXCMS10.one.ads.che.org> <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C93@CHEXCMS10.one.ads.che.org> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2441E@PHSXMB30.partners.org> We have been using them (24x50) and have had no problems with broken glass pieces. I do agree that they seem "dirty". Never contacted them about it. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 3:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Coverslips I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From sgoebel <@t> xbiotech.com Mon Jun 28 14:41:44 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Jun 28 14:41:53 2010 Subject: [Histonet] RE: Coverslips Message-ID: <20100628124144.9e2d9aa830e8449a2412eb1e4f2f067e.fdd3de83db.wbe@email04.secureserver.net> I just got a box of these as a "trial". They all have been far (used 1/2 the box so far)? Maybe there is gunk in your co verslipper that is causing this or possibly some glass particles? I c Sarah Goebel, B.A., HT (ASCP) < Histotechnicia XBiotech USA Inc. < Austin, Texas 78744 < (512) -------- Original Message -------- Subject: [Histonet] RE: Coverslips From: "Weems, Joyce" <[1]JWeems@sjha.o Date: Mon, June 28, 2010 12:06 pm To: "[2]histonet@lists. <[3]histonet@lists.u I forgot to say that the packaging has changed to their Histo Hippo theme, -----Original Message----- From: [4]histon [[5]mailto:histonet-bounces@lists.utsouthwestern.edu Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: [6]histonet@lists.u Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white with them air bubbles when us having this problem? They and have replaced several boxes, same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health E recipient(s). It may contain information that is privileged and confidential. Any unauth If you are n and reply to the sen _______________________________________________ Histonet mailing list [7]Histonet@lists.utsou [8]http: Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list [9]Histonet@lists.utsou [10]http: References 1. 3D"mailto://JWeems@sjha.org"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 4. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 5. 3D"mailto:histonet-bounces 6. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 7. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 8. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 9. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Janice.Mahoney <@t> alegent.org Mon Jun 28 14:48:44 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Mon Jun 28 14:49:01 2010 Subject: [Histonet] Tissue Processors In-Reply-To: <73A7ED895EE0C24D9267ED814911DF1912B75511@exchange.cmc-nh.org> References: <748458.69728.qm@web112603.mail.gq1.yahoo.com> <73A7ED895EE0C24D9267ED814911DF1912B75511@exchange.cmc-nh.org> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2CC@EXCHMBC2.ad.ah.local> I second Steve's comments about the Peloris. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, June 28, 2010 1:56 PM To: mohamed abd el razik; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processors We have 2 Peloris processors and run protocols ranging from 2 hrs to overnight. Several 2 and 4 hour protocols daily keeps a constant flow of specimens moving through the lab for those interested in LEAN small batch processing. As far as programmability, it 's all touch screen driven and easy. It's a smart processor so we don't change all solutions weekly. We only change the ones that are needed. The parameters that determine how long to use a particular solution are also programmable. Changing solutions is done by pumping out of the individual bottles and into waste containers and refilling is pumped from clean containers of solution back into the original containers. No heavy lifting required. Ours are in a separate room from our microtomy area so we hooked a simple door bell up to the local alarm jack on the back. When processing is done, we get the appropriate tone. Remote alarm is also tied in to the hospital switchboard in case a processor goes down at night or during the weekend. We really like the versatility and dependability of these processors. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Saturday, June 26, 2010 3:41 PM To: Histonet@lists.utsouthwestern.edu Subject: Fw: [Histonet] Tissue Processors i need it too as we are going to bring new tissue processor to our small lab --- On Sat, 6/26/10, Shirley Pan wrote: From: Shirley Pan Subject: [Histonet] Tissue Processors To: histonet@lists.utsouthwestern.edu Date: Saturday, June 26, 2010, 7:55 AM We are in the process of trying out tissue processors. Are there any users of the Leica Peloris or Thermo EG who can help us out with some opinions? Reliability, ease of changing solutions, programmability? Thanks for any help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From JWeems <@t> sjha.org Mon Jun 28 15:04:27 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Jun 28 15:04:32 2010 Subject: [Histonet] RE: Coverslips In-Reply-To: <20100628124144.9e2d9aa830e8449a2412eb1e4f2f067e.fdd3de83db.wbe@email04.secureserver.net> References: <20100628124144.9e2d9aa830e8449a2412eb1e4f2f067e.fdd3de83db.wbe@email04.secureserver.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5CBA@CHEXCMS10.one.ads.che.org> We can coverslip by hand, but soon after the slides are coverslipped on the machine, air bubbles begin to form. We we coverslip them by hand, there is visible glass that we can feel and move if we need too. They've been good for so long, I want to make sure it's not just us. Thanks for the feedback! j ________________________________ From: sgoebel@xbiotech.com [mailto:sgoebel@xbiotech.com] Sent: Monday, June 28, 2010 15:42 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Coverslips I just got a box of these as a "trial". They all have been fine so far (used 1/2 the box so far)? Maybe there is gunk in your coverslipper that is causing this or possibly some glass particles? I coverslip by hand. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] RE: Coverslips From: "Weems, Joyce" > Date: Mon, June 28, 2010 12:06 pm To: "histonet@lists.utsouthwestern.edu" > I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rjbuesa <@t> yahoo.com Mon Jun 28 15:19:18 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 28 15:19:27 2010 Subject: [Histonet] Unstained Slides In-Reply-To: Message-ID: <946724.39272.qm@web65713.mail.ac4.yahoo.com> Depending on the?epitope you are trying to detect, the time could be limited?from less than one month to 1-2 years. Ren? J. --- On Mon, 6/28/10, Rick.Garnhart@memorialhealthsystem.com wrote: From: Rick.Garnhart@memorialhealthsystem.com Subject: [Histonet] Unstained Slides To: histonet@lists.utsouthwestern.edu Date: Monday, June 28, 2010, 11:54 AM Histoland, How? is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph:? 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Mon Jun 28 15:58:48 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Jun 28 15:58:54 2010 Subject: [Histonet] Inexpensive fume hood? Message-ID: Can anyone recommend an inexpensive fume hood/ workstation? Not the full on metal type that hangs from the ceiling, but the Lucite bench top unit with a carbon filter. I want to avoid the capital equipment rigmarole, so it has to cost less than $1000. It is to put a small automated coverslipper under, 16" H x 20" W x 10" D . Thanks, Jay A. Lundgren, M.S., HTL (ASCP) From foreightl <@t> gmail.com Mon Jun 28 16:04:42 2010 From: foreightl <@t> gmail.com (Pat Laurie) Date: Mon Jun 28 16:04:47 2010 Subject: [Histonet] Tissue Processors In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2CC@EXCHMBC2.ad.ah.local> References: <748458.69728.qm@web112603.mail.gq1.yahoo.com> <73A7ED895EE0C24D9267ED814911DF1912B75511@exchange.cmc-nh.org> <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2CC@EXCHMBC2.ad.ah.local> Message-ID: I 3rd the previous statements about the Peloris. I had 2 pelori at my previous job, and now we currently have 3 pelori. We are also looking at getting a 4th. We use them regularly and due to the ability to use shorter programs, we run about 15 runs a day between them while still having significant downtime. I must warn you though, it is necessary to keep the service contracts on them. They are about 99% computer and have a significant number of moving parts, but they do work wonderfully. On Mon, Jun 28, 2010 at 12:48 PM, Mahoney,Janice A < Janice.Mahoney@alegent.org> wrote: > I second Steve's comments about the Peloris. > Jan Mahoney > Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen > Sent: Monday, June 28, 2010 1:56 PM > To: mohamed abd el razik; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Tissue Processors > > We have 2 Peloris processors and run protocols ranging from 2 hrs to > overnight. Several 2 and 4 hour protocols daily keeps a constant flow of > specimens moving through the lab for those interested in LEAN small batch > processing. As far as programmability, it 's all touch screen driven and > easy. It's a smart processor so we don't change all solutions weekly. We > only change the ones that are needed. The parameters that determine how > long to use a particular solution are also programmable. Changing solutions > is done by pumping out of the individual bottles and into waste containers > and refilling is pumped from clean containers of solution back into the > original containers. No heavy lifting required. > > Ours are in a separate room from our microtomy area so we hooked a simple > door bell up to the local alarm jack on the back. When processing is done, > we get the appropriate tone. Remote alarm is also tied in to the hospital > switchboard in case a processor goes down at night or during the weekend. > > We really like the versatility and dependability of these processors. > > > Steve > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el > razik > Sent: Saturday, June 26, 2010 3:41 PM > To: Histonet@lists.utsouthwestern.edu > Subject: Fw: [Histonet] Tissue Processors > > > i need it too as we are going to bring new tissue processor to our small > lab > --- On Sat, 6/26/10, Shirley Pan wrote: > > > From: Shirley Pan > Subject: [Histonet] Tissue Processors > To: histonet@lists.utsouthwestern.edu > Date: Saturday, June 26, 2010, 7:55 AM > > > We are in the process of trying out tissue processors. Are there any users > of the Leica Peloris or Thermo EG who can help us out with some opinions? > Reliability, ease of changing solutions, programmability? Thanks for any > help. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is > faithful to the healing ministry of Jesus Christ, providing high quality > care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is > confidential and private and intended only for the use of the addressees. > Unauthorized use, disclosure, distribution or copying is strictly > prohibited and may be unlawful. If you received this communication in > error, please inform us of the erroneous delivery by return e-mail message > from your computer. Additionally, although all attachments have been > scanned at the source for viruses, the recipient should check any > attachments for the presence of viruses before opening. Alegent Health > accepts no liability for any damage caused by any virus transmitted by this > e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com From aamador <@t> ameripath.com Mon Jun 28 17:57:37 2010 From: aamador <@t> ameripath.com (Amador, Amanda) Date: Mon Jun 28 17:57:43 2010 Subject: [Histonet] Modified Genta Message-ID: <1D9B439FC446244095353DCA4FC23DD20454E1D27B@MWNMAIL00.ameripath.local> Is there someone that could help out with the Modified Genta? We tried it in the microwave and we are not sure if we are doing it correctly. We would appreciate any sample procedures to see if we need to change something. Thanks to anyone who can help out. aamador@ameripath.com From mcauliff <@t> umdnj.edu Tue Jun 29 08:53:10 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jun 29 08:50:44 2010 Subject: [Histonet] what do you think? In-Reply-To: <602647.18150.qm@web112609.mail.gq1.yahoo.com> References: <602647.18150.qm@web112609.mail.gq1.yahoo.com> Message-ID: <4C29FAC6.1030909@umdnj.edu> Greetings Mohamed: The lamina propria brings small blood vessels and lymphatics close to the epithelium lining the large and small intestines. This allows nutrients to be passed back and forth and for the immune system to monitor any antigens that cross the epithelium. The relationship between the immune system and the contents of the GI tract is very complex. Lymph nodules in the lamina propria would make it thicker in some regions than in other regions. Whether the thickening you refer to is pathological or just the normal response to a variety of factors is difficult to say. Geoff mohamed abd el razik wrote: > dear histonetters > i have a quistion please regarding the thickness of lamina propria in small or larg intestine. > what is the significant of it?? does it mean inflamation and pathological condition or mean more size and so absorption of nutrients? i mean does the thickning is favorable or not? > > thanks > Mohamed Abd Elrazik > Histology dep. > Fac. of Vet. Med. > Cairo . Univ. -Egypt > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From abijag76 <@t> rediffmail.com Tue Jun 29 08:59:09 2010 From: abijag76 <@t> rediffmail.com (abijag ) Date: Tue Jun 29 08:59:34 2010 Subject: [Histonet] Your experience with microm HMS 740 auto stainer Message-ID: <1277819686.S.1418.34634.webmail.rediff.com.drafts.1277819949.50235@webmail.rediffmail.com> Dear Histonetters, We would like to procure one tissue auto stainer for our histology lab(mainly H&E staining) In this respect, I request your experience about Microm HMS 740 automatic stainer. Please share your comments regarding the staining reproducibility,ease of handling and their claim about drip prevention technology. Based on your valuable feedback, we will make our decision. Thanks for all help Abi jagannath From rjbuesa <@t> yahoo.com Tue Jun 29 10:20:32 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 29 10:20:36 2010 Subject: [Histonet] Inexpensive fume hood? In-Reply-To: Message-ID: <364541.78070.qm@web65712.mail.ac4.yahoo.com> Check a Fisher catalog. The ones they sell are good for your purposes. Ren? J. --- On Mon, 6/28/10, Jay Lundgren wrote: From: Jay Lundgren Subject: [Histonet] Inexpensive fume hood? To: "histonet" Date: Monday, June 28, 2010, 4:58 PM Can anyone recommend an inexpensive fume hood/ workstation?? Not the full on metal type that hangs from the ceiling, but the Lucite bench top unit with a carbon filter.? I want to avoid the capital equipment rigmarole, so it has to cost less than $1000.? It is to put a small automated coverslipper under,? 16" H x 20" W x 10" D . ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???Thanks, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Jun 29 10:53:32 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Tue Jun 29 10:53:47 2010 Subject: [Histonet] New CAP question ANP.22760 In-Reply-To: <90354A475B420441B2A0396E5008D49692BF70@copc-sbs.COPC.local> References: <12347581.1277575674701.JavaMail.root@elwamui-cypress.atl.sa.earthlink.net> <90354A475B420441B2A0396E5008D49692BF70@copc-sbs.COPC.local> Message-ID: <1AAF670737F193429070841C6B2ADD4C021C3610F1@EXMBMCB15.ucsfmedicalcenter.org> Thomas and Daniel both make good points. While clinical labs do run measurable (quantitative) tests they are also running completely closed systems in which all reagents are from a certain vendor. All the reagents are validated by a vendor and all reagents are validated and calibrated for that system. Running qualitative tests is only different at the interpretive stage. All other stages should be standardized and validated to ensure the system works as intended every day, every test. IHC, of course, is a whole different ballgame. There probably is not a single lab out there that uses only one vendor and uses only one system to do IHC testing. Even if you use one automated staining system you may use antibodies from a different vendor, or you may do something outside the system that is very different than other labs use. There is such disparity between testing in different labs that it is difficult to compare results and even techniques between labs. That is the crux of the issue and what CAP and others are trying to address by tightening the validation screws. In the near future labs will need to have very robust validation and documentation for all antibodies in order to prove they are doing things correctly. That scenario is already here for the breast markers. It will come for the others as well. Right now the instances in which solid validation methods are absolutely necessary is when validating the breast markers ER, PR and HER2, which all are used as stand-alone tests to determine treatment, and for ASR's. If you use a vendor kit with all reagents supplied you must still prove it works in your lab as intended ("verify outside data." The "outside data" is the claim that it stains in a certain way). You must run cases of various expressions and show your lab gets the proper results. Third party verification is very helpful, that is, comparing your results to that of other labs on the same material (CAP surveys, or set up a partnership with another lab). If you use only the antibody for one of those markers, or mix and match components outside a FDA-approved kit, then you are responsible for performing a comprehensive validation of the test. Its worth noting that every news article I've seen about pathology/histology labs in the last several years has been about failures to do the breast panel tests correctly. We are under a microscope these days and it will pay off to make sure you are doing the tests well! Most other markers in IHC are ancillary tests that are run in conjunction with other tests to determine a diagnosis. Those antibodies still need to be validated in your system but don't require a large sample of cases. They have been validated as IVD's by the vendor so just need to be shown they work as intended with your lab's methods and instruments. But I still think it is worthwhile to do a validation that includes a range of expressions along with irrelevant tissue (negative). That will help calibrate your expectations about how the antibody works and give you a baseline to refer to if problems arise. Running one or two slides to make sure it "works" is not really satisfactory. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, June 26, 2010 1:45 PM To: Daniel Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Daniel, I think your observations are very good. Which brings me again to a point I was trying to make earlier. Anatomic Pathology is not the same as the General Clinical Lab or it's close counterparts. It almost seems like this is overblown re: validation. As you've pointed out, the interpretation is up to the pathologist. Patients (prostate, i.e.) will correlate with other tests. When known positives demonstrate properly you're valid. Even automated systems can use algorithms to score strength of positivity...which varies yet will show as positive (valid). You receive a new batch of Ab you run it on a known positive, that's the bottom line. It seems somehow CAP or clinical lab (trained) folks want more than that. And again (as you pointed out) the appreciation for the differences in how we operate as laboratorians seems to fall by the wayside. As you mention pushing 20, 50, 100 is not feasible (and frankly unnecessary). I don't know where the answer lies in making everyone happy here. I do know that some thinking outside the box and trying understand AP is required. We all want good patient care, but one size does not fit all. Thanks, tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Sent: Saturday, June 26, 2010 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 One question I think a lot of you are not considering is that the clinical laboratory usually tests for analytes present in most patients. This allows the clinical lab to more easily run validations with hundreds of patients, using statistical tools to analyze the precision, specificity and sensitivity of the test. What most of those concerned with this new CAP standard (and I count among them) is that our testing often targets a very rare population of patients. As such, an extensive validation is much more difficult to establish. It then makes the statistical models used in clinical tests much less valuble since the accuracy of these formulas decrease with smaller sample sizes. Additionally, the pathology of the clinical tests are reported as a measurement which does not have an intrinsic value. It must be interpreted by a clinician to give value to the patient. For example, the Blood Urea Nitrogen test value range usually is between 7-20 mg/dL while values above 50 are considered a marker of poor kidney function. Elevated values, however, do not mean that kidney functions are impaired but instead may be due to other pathologies such as congestive heart failure, gi bleeding, certain steroids and even diet. For histopathology even relatively common antibodies such as pan-T cell marker CD3 usually only stain 75% of T cell neoplasms. A negative result for that minority does not indicate that the tumor does not have a T cell lineage. It relies instead on the pathologist interpreting the result much as the clinician looks at the BUN value in relation to other factors in the patient's presentation. When we validate this kind of antibody, do we perform testing then on T cells and B cells (give me a good tonsil), on malignant samples or some combination of the two? What is a reasonable number of tests to run then? If I choose 10 normal lymphatic tissue blocks for my routine T/B cell marking (it can't be as extensive as ER/PR can it?) how many samples should I choose for my malignant population? If I use another 10 anyone with a background in statistics will tell you that the sample size is too small to interpret accuracy. If I push it up to 20, 50 or 100 I would be certain that many laboratories would not be able to afford the cost of the validation. This would then push many good laboratories out of the business of IHC with the unintended result of delaying diagnoses and increasing patient costs by driving testing to fewer and fewer testing outlets. CAP's new path with ER/PR seems to be trying to achieve a noble end of improving quality in the laboratory but without an understanding of the complexities and consequences of the method they are implementing. Dan -----Original Message----- >From: Jesus Ellin >Sent: Jun 26, 2010 6:28 AM >To: "Morken, Tim" , >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >I have been reading the post to this question and it seems to me that there are different standards depending on the lab that is operating the methodology. I do agree that the core lab for years have had the instruction and training in the performance of validation. One thing that comes to mind as well is why has histology not had this training? Why are we not getting this from our certification agency, our professional societies and biggest reason where is our standardization. It seems to me that with all these regualtions in plac for so long, why were we missed. Is it because when inspected through CAP we are being inspected by a pathologist rather than a histo tech? These are some of the questions at hand. I to see new standards within the CAP checklist as well as other regulatory organizations that will affect the future of the Anatomic Pathology community. But I think we need is to provide a underlying architecture for our peers, so that we can begin the transition to the future. This is only the beginning, there is still Digital Image Analysis and Telepathology. It funny we are looking to become a hybrid of radiology and the core lab, but with the best of both worlds. Tim great structure for the validation study. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, >Tim >Sent: Wed 6/23/2010 9:48 AM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Joe, > >You wrote : The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time ..." > >Those were my thoughts exactly. While the person replying may or may not have specific histology experience she will have clinical lab experience (however, my guess is that she is exposed to histology regularly at CAP). Clinical labs have a bit of an easier time, actually, because they validate primarily to known concentration controls - analytical controls manufactured at a range of known concentrations for instance. The institution then adds in their normal controls for validation. > >As far as the current question about validating a new lot of reagent the best practice is to run parallel tests on the same machine. If that is not easily possible on a particular manufacturer's instrument then the question should be asked of them: Why not? If this is a requirement the manufacturer should provide an easy path to meeting the requirement. However, if that is not the case then the institution simply writes a procedure to get around the inadequacies of the instrument (Maybe the vendor can help with that). Then follow the procedure. That should satisfy inspectors. > >An "...appropriate panel of tissues..." is whatever the institution deems appropriate for the given antibody or reagent. This is a perfect place for tissue arrays. You can make your own or buy them. > >IHC must meet CLIA validation guidelines but since IHC is generally qualitative the requirements must be understood and methods adapted to a qualitative scenario. Several IHC and Histotechnology books discuss the subject at length (Taylor, Dabbs, Bancroft for instance). > >Below is a brief overview of how to do that. (for more in-depth info this was covered in an NSH teleconference I gave last year - PowerPoint, audio and references available from NSH-, and will be covered in a similar workshop at NSH in Seattle this year). > > >1) >CAP General Validation >CAP GEN.42020-42163 Test Method Validation Follows CLIA CFR Sec >493.1253 Does not apply well to IHC (IHC is usually qualitative) > >But the general principle applies: >The laboratory must have data on each test's accuracy, precision, analytic sensitivity, interferences and reportable range. > >Unmodified FDA-cleared or approved tests: the lab may use manufacturer information or published reports but lab must verify outside data. > >Non-FDA cleared: Lab MUST verify or establish analytic accuracy, precision, sensitivity, specificity and reportable range. > >2) Validation includes: >Accuracy: > Compare results with New antibody to a previously validated antibody on the same tissues > >Precision: > Test samples with varying antigen expression > Intra-run, Inter-run tests, 10 slides each (reproducibility) > >Sensitivity: > True Positive vs False Negative (higher % FN = less sensitive) > >Interferences [Specificity]: > True Negative vs False Positive (Higher % FP = less specific) > Delineate what could interfere to give a false positive or false negative result. > >Reportable Range > Establish a scoring system > Provide the definition of a positive result > >3)Sensitivity > >Analytic Sensitivity: > Lowest amount of substance detectable by the test > Can only be done with controls of known concentration > >Diagnostic Sensitivity: > Ability of the test to determine true diagnostic positive verses false negative (higher % FN = less sensitive) > Requires comparison to a previously validated antibody > >IHC Sensitivity: > Extent to which an antibody can be diluted and still achieve target recognition. NOTE: This is determined by antibody AND detection system! > > > >4) Specificity: > >Analytic Specificity > Accuracy on tests of known positive and negative controls > Controls of known concentration > Determine what could "Interfere" to confound the result > > >Diagnostic Specificity > Ability of a test to determine true diagnostic negative verses false positives (Higher % FP = less specific) > Requires comparison to a previously validated antibody > > >IHC Specificity > Ability of an antibody to bind exclusively to its particular antigen in the absence of staining of other molecules > Or, staining of other structures in addition to target >structures/cells > >(Sensitivity and Specificity adapted from: Theoretical and Practical >Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote, 2005) > >Tim Morken >Supervisor, Histology / IPOX >UCSF Medical Center >San Francisco, CA > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >JMyers1@aol.com >Sent: Tuesday, June 22, 2010 6:51 PM >To: tjasper@copc.net >Cc: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Tom: > >As much as I agree with your acknowledgment that its seems a bit odd >for the CAP to have a blood-banker responding to AP-related issue, I'm >actually not surprised. The folks in the 'clinical' lab have been >performing more comprehensive and complex validation procedures for a >very long time, and they wonder why IHC isn't expected to follow the >same requirements as chemistry, immunology, etc. -- IHC is, after all, >an awful lot like ELISA. And rightfully so, because IHC is, under CLIA >(which supersedes CAP), considered highly-complex, non-waived testing >-- and is, therefore, subject to the same Quality Systems regulations >(in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. > >Could it be that, because AP produces qualitative results that are >interpreted by a pathologist and CP produces quantitative results that >are interpreted by an analyzer, we somehow think that CLIA rules don't >apply to IHC? I certainly don't have the answer to that, but it make >me wonder what the future holds. As witnessed by some of the newest >CAP 'standards' (including the question in question...no pun intended), >e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens >must be tested, and where 10 of the positives must be weakly positive >-- an acknowledgment that validation specimens must be carefully >selected in order to obtain appropriate results), it certainly doesn't >appear that the regulation of IHC testing is going to become more relaxed. > >Joe Myers, M.S., CT(ASCP) > >------------------------------ > >Message: 12 >Date: Fri, 18 Jun 2010 12:38:07 -0700 >From: "Thomas Jasper" >Subject: RE: [Histonet] New CAP question ANP.22760 >To: "Mark Tarango" >Cc: _histonet@lists.utsouthwestern.edu_ >(mailto:histonet@lists.utsouthwestern.edu) > >Mark, > >Did you notice the credentials from this CAP representative? MT with a >Blood Bank specialty I believe. What I glean from that is...more than >likely this person does not grasp the logistics of "contemporaneously" >staining identical Abs from separate lots. She also likely does not >understand the logistical application for detection and automation >either. > >I'm not trying to be overly critical of this person. I'm sure she is >quite intelligent and would not have the MT/SBB if she wasn't >intelligent. It comes down to a lack of understanding Anatomic >Pathology testing application re: automated IHC. I believe this is a >common problem in and out of CAP. Many lab directors and other folks in >positions of authority without AP/Histology/Cytology backgrounds seem >to believe that broad clinical lab modalities apply to Anatomic Path >scenarios. I used to refer to this in my former position as - "Trying >to put the yoke of clinical lab onto anatomic path." We are >laboratorians, but in many instances do not fit the general clinical >lab mold. > >It's unfortunate that CAP has put this person in the position to >respond. It is apparent to me that she's not grasping the particulars >here. She probably never will unless she decides to go into a working, >automated IHC "tissue" lab and take the time to ask questions and >understand (learn) what we're all about. > >Thanks, >Tom Jasper > >Thomas Jasper HT (ASCP) BAS >Histology Supervisor >Central Oregon Regional Pathology Services Bend, OR 97701 >_______________________________________________ From emlynda <@t> pathology.ufl.edu Tue Jun 29 11:17:58 2010 From: emlynda <@t> pathology.ufl.edu (Schneider,Lynda S) Date: Tue Jun 29 11:18:06 2010 Subject: [Histonet] Canine bone sectioning trouble Message-ID: <94A0BFCE84CB0F4A84C50EAC9B02D0A9019423208F@HSC-CMS01.ad.ufl.edu> Hello out there... We are having trouble sectioning canine bone. The samples are bone marrow cubes approximately 2cm thick and 1in wide. They were fixed for 15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid decalcifier. They were faced in and then surface decaled again for about 30 mins. When sectioned, much of the marrow was missing and the bone was torn and shredded. We thought that maybe the samples had not been fixed sufficiently so refixed overnight in 10% NBF again. The samples were then reprocessed as they had been originally. The processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each, paraffin x 4, 40 mins each. Again they were faced in and decaled for another 30 mins or so. This time as soon as the sections are placed on the water bath (38 degrees) they explode and/or come apart so severely sections can almost not be picked up. If sections are even obtainable they are of horrible quality. Does anyone have any suggestions? Thank you so much in advance! Lynda From BenatM <@t> gosh.nhs.uk Tue Jun 29 11:53:26 2010 From: BenatM <@t> gosh.nhs.uk (Malika Benatti) Date: Tue Jun 29 11:54:19 2010 Subject: [Histonet] RE: Coverslips In-Reply-To: <201006291613.JMV51864@rlmh06.swi.contact.secure-ops.net> References: <201006291613.JMV51864@rlmh06.swi.contact.secure-ops.net> Message-ID: <4C2A3319.4626.0038.0@gosh.nhs.uk> ** Proprietary ** ** Reply Requested When Convenient ** Hi there, We use the CellPath CoverSlips 22x50mm No 1.5 they are great for hand mounting but when used with the Leica CV5030 they are a real pain. most of the CoversSlips get rejected. If anyone use the same equipment, let me know how you are getting one . Cheers, Malika ------------------------------ Message: 7 Date: Mon, 28 Jun 2010 15:06:08 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: Coverslips To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C93@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 8 Date: Mon, 28 Jun 2010 12:21:33 -0700 From: "Kathleen Boozer" Subject: Re: [Histonet] Coverslips To: "histonet@lists.utsouthwestern.edu" , "Joyce Weems" Message-ID: <4C2893CC.4AA8.00C0.1@ah.org> Content-Type: text/plain; charset=US-ASCII I am having the same issues and we purchased the expensive "Platinum" to avoid that problem. They just sent me replacements and I haven't tested them all yet. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk >>> "Weems, Joyce" 6/28/2010 11:28 AM >>> For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 28 Jun 2010 15:36:22 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] RE: Coverslips To: "Weems, Joyce" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2441E@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" We have been using them (24x50) and have had no problems with broken glass pieces. I do agree that they seem "dirty". Never contacted them about it. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 3:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Coverslips I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 10 Date: Mon, 28 Jun 2010 12:41:44 -0700 From: sgoebel@xbiotech.com Subject: RE: [Histonet] RE: Coverslips To: "Weems,Joyce" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <20100628124144.9e2d9aa830e8449a2412eb1e4f2f067e.fdd3de83db.wbe@email04.secureserver.net> Content-Type: text/plain; charset="utf-8" I just got a box of these as a "trial". They all have been far (used 1/2 the box so far)? Maybe there is gunk in your co verslipper that is causing this or possibly some glass particles? I c Sarah Goebel, B.A., HT (ASCP) < Histotechnicia XBiotech USA Inc. < Austin, Texas 78744 < (512) -------- Original Message -------- Subject: [Histonet] RE: Coverslips From: "Weems, Joyce" <[1]JWeems@sjha.o Date: Mon, June 28, 2010 12:06 pm To: "[2]histonet@lists. <[3]histonet@lists.u I forgot to say that the packaging has changed to their Histo Hippo theme, -----Original Message----- From: [4]histon [[5]mailto:histonet-bounces@lists.utsouthwestern.edu Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: [6]histonet@lists.u Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white with them air bubbles when us having this problem? They and have replaced several boxes, same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health E recipient(s). It may contain information that is privileged and confidential. Any unauth If you are n and reply to the sen _______________________________________________ Histonet mailing list [7]Histonet@lists.utsou [8]http: Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list [9]Histonet@lists.utsou [10]http: References 1. 3D"mailto://JWeems@sjha.org"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 4. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 5. 3D"mailto:histonet-bounces 6. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 7. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 8. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 9. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" ------------------------------ Message: 11 Date: Mon, 28 Jun 2010 14:48:44 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] Tissue Processors To: "'Feher, Stephen'" , mohamed abd el razik , "Histonet@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2CC@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="iso-8859-1" I second Steve's comments about the Peloris. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, June 28, 2010 1:56 PM To: mohamed abd el razik; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processors We have 2 Peloris processors and run protocols ranging from 2 hrs to overnight. Several 2 and 4 hour protocols daily keeps a constant flow of specimens moving through the lab for those interested in LEAN small batch processing. As far as programmability, it 's all touch screen driven and easy. It's a smart processor so we don't change all solutions weekly. We only change the ones that are needed. The parameters that determine how long to use a particular solution are also programmable. Changing solutions is done by pumping out of the individual bottles and into waste containers and refilling is pumped from clean containers of solution back into the original containers. No heavy lifting required. Ours are in a separate room from our microtomy area so we hooked a simple door bell up to the local alarm jack on the back. When processing is done, we get the appropriate tone. Remote alarm is also tied in to the hospital switchboard in case a processor goes down at night or during the weekend. We really like the versatility and dependability of these processors. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Saturday, June 26, 2010 3:41 PM To: Histonet@lists.utsouthwestern.edu Subject: Fw: [Histonet] Tissue Processors i need it too as we are going to bring new tissue processor to our small lab --- On Sat, 6/26/10, Shirley Pan wrote: From: Shirley Pan Subject: [Histonet] Tissue Processors To: histonet@lists.utsouthwestern.edu Date: Saturday, June 26, 2010, 7:55 AM We are in the process of trying out tissue processors. Are there any users of the Leica Peloris or Thermo EG who can help us out with some opinions? Reliability, ease of changing solutions, programmability? Thanks for any help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. 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Thank you for your cooperation. ------------------------------ Message: 12 Date: Mon, 28 Jun 2010 16:04:27 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] RE: Coverslips To: "sgoebel@xbiotech.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5CBA@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We can coverslip by hand, but soon after the slides are coverslipped on the machine, air bubbles begin to form. We we coverslip them by hand, there is visible glass that we can feel and move if we need too. They've been good for so long, I want to make sure it's not just us. Thanks for the feedback! j ________________________________ From: sgoebel@xbiotech.com [mailto:sgoebel@xbiotech.com] Sent: Monday, June 28, 2010 15:42 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Coverslips I just got a box of these as a "trial". They all have been fine so far (used 1/2 the box so far)? Maybe there is gunk in your coverslipper that is causing this or possibly some glass particles? I coverslip by hand. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] RE: Coverslips From: "Weems, Joyce" > Date: Mon, June 28, 2010 12:06 pm To: "histonet@lists.utsouthwestern.edu" > I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 13 Date: Mon, 28 Jun 2010 13:19:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Unstained Slides To: histonet@lists.utsouthwestern.edu, Rick.Garnhart@memorialhealthsystem.com Message-ID: <946724.39272.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Depending on the?epitope you are trying to detect, the time could be limited?from less than one month to 1-2 years. Ren? J. --- On Mon, 6/28/10, Rick.Garnhart@memorialhealthsystem.com wrote: From: Rick.Garnhart@memorialhealthsystem.com Subject: [Histonet] Unstained Slides To: histonet@lists.utsouthwestern.edu Date: Monday, June 28, 2010, 11:54 AM Histoland, How? is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph:? 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 28 Jun 2010 14:58:48 -0600 From: Jay Lundgren Subject: [Histonet] Inexpensive fume hood? To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Can anyone recommend an inexpensive fume hood/ workstation? Not the full on metal type that hangs from the ceiling, but the Lucite bench top unit with a carbon filter. I want to avoid the capital equipment rigmarole, so it has to cost less than $1000. It is to put a small automated coverslipper under, 16" H x 20" W x 10" D . Thanks, Jay A. Lundgren, M.S., HTL (ASCP) ------------------------------ Message: 15 Date: Mon, 28 Jun 2010 14:04:42 -0700 From: Pat Laurie Subject: Re: [Histonet] Tissue Processors To: "Mahoney,Janice A" Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I 3rd the previous statements about the Peloris. I had 2 pelori at my previous job, and now we currently have 3 pelori. We are also looking at getting a 4th. We use them regularly and due to the ability to use shorter programs, we run about 15 runs a day between them while still having significant downtime. I must warn you though, it is necessary to keep the service contracts on them. They are about 99% computer and have a significant number of moving parts, but they do work wonderfully. On Mon, Jun 28, 2010 at 12:48 PM, Mahoney,Janice A < Janice.Mahoney@alegent.org> wrote: > I second Steve's comments about the Peloris. > Jan Mahoney > Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen > Sent: Monday, June 28, 2010 1:56 PM > To: mohamed abd el razik; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Tissue Processors > > We have 2 Peloris processors and run protocols ranging from 2 hrs to > overnight. Several 2 and 4 hour protocols daily keeps a constant flow of > specimens moving through the lab for those interested in LEAN small batch > processing. As far as programmability, it 's all touch screen driven and > easy. It's a smart processor so we don't change all solutions weekly. We > only change the ones that are needed. The parameters that determine how > long to use a particular solution are also programmable. Changing solutions > is done by pumping out of the individual bottles and into waste containers > and refilling is pumped from clean containers of solution back into the > original containers. No heavy lifting required. > > Ours are in a separate room from our microtomy area so we hooked a simple > door bell up to the local alarm jack on the back. When processing is done, > we get the appropriate tone. Remote alarm is also tied in to the hospital > switchboard in case a processor goes down at night or during the weekend. > > We really like the versatility and dependability of these processors. > > > Steve > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el > razik > Sent: Saturday, June 26, 2010 3:41 PM > To: Histonet@lists.utsouthwestern.edu > Subject: Fw: [Histonet] Tissue Processors > > > i need it too as we are going to bring new tissue processor to our small > lab > --- On Sat, 6/26/10, Shirley Pan wrote: > > > From: Shirley Pan > Subject: [Histonet] Tissue Processors > To: histonet@lists.utsouthwestern.edu > Date: Saturday, June 26, 2010, 7:55 AM > > > We are in the process of trying out tissue processors. Are there any users > of the Leica Peloris or Thermo EG who can help us out with some opinions? > Reliability, ease of changing solutions, programmability? Thanks for any > help. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is > faithful to the healing ministry of Jesus Christ, providing high quality > care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is > confidential and private and intended only for the use of the addressees. > Unauthorized use, disclosure, distribution or copying is strictly > prohibited and may be unlawful. If you received this communication in > error, please inform us of the erroneous delivery by return e-mail message > from your computer. Additionally, although all attachments have been > scanned at the source for viruses, the recipient should check any > attachments for the presence of viruses before opening. Alegent Health > accepts no liability for any damage caused by any virus transmitted by this > e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com ------------------------------ Message: 16 Date: Mon, 28 Jun 2010 17:57:37 -0500 From: "Amador, Amanda" Subject: [Histonet] Modified Genta To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <1D9B439FC446244095353DCA4FC23DD20454E1D27B@MWNMAIL00.ameripath.local> Content-Type: text/plain; charset="us-ascii" Is there someone that could help out with the Modified Genta? We tried it in the microwave and we are not sure if we are doing it correctly. We would appreciate any sample procedures to see if we need to change something. Thanks to anyone who can help out. aamador@ameripath.com ------------------------------ Message: 17 Date: Tue, 29 Jun 2010 09:53:10 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] what do you think? To: mohamed abd el razik Cc: Histonet@lists.utsouthwestern.edu Message-ID: <4C29FAC6.1030909@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Greetings Mohamed: The lamina propria brings small blood vessels and lymphatics close to the epithelium lining the large and small intestines. This allows nutrients to be passed back and forth and for the immune system to monitor any antigens that cross the epithelium. The relationship between the immune system and the contents of the GI tract is very complex. Lymph nodules in the lamina propria would make it thicker in some regions than in other regions. Whether the thickening you refer to is pathological or just the normal response to a variety of factors is difficult to say. Geoff mohamed abd el razik wrote: > dear histonetters > i have a quistion please regarding the thickness of lamina propria in small or larg intestine. > what is the significant of it?? does it mean inflamation and pathological condition or mean more size and so absorption of nutrients? i mean does the thickning is favorable or not? > > thanks > Mohamed Abd Elrazik > Histology dep. > Fac. of Vet. Med. > Cairo . Univ. -Egypt > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 18 Date: 29 Jun 2010 13:59:09 -0000 From: "abijag " Subject: [Histonet] Your experience with microm HMS 740 auto stainer To: Message-ID: <1277819686.S.1418.34634.webmail.rediff.com.drafts.1277819949.50235@webmail.rediffmail.com> Content-Type: text/plain; charset="UTF-8" Dear Histonetters, We would like to procure one tissue auto stainer for our histology lab(mainly H&E staining) In this respect, I request your experience about Microm HMS 740 automatic stainer. Please share your comments regarding the staining reproducibility,ease of handling and their claim about drip prevention technology. Based on your valuable feedback, we will make our decision. Thanks for all help Abi jagannath ------------------------------ Message: 19 Date: Tue, 29 Jun 2010 08:20:32 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Inexpensive fume hood? To: histonet , Jay Lundgren Message-ID: <364541.78070.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Check a Fisher catalog. The ones they sell are good for your purposes. Ren? J. --- On Mon, 6/28/10, Jay Lundgren wrote: From: Jay Lundgren Subject: [Histonet] Inexpensive fume hood? To: "histonet" Date: Monday, June 28, 2010, 4:58 PM Can anyone recommend an inexpensive fume hood/ workstation?? Not the full on metal type that hangs from the ceiling, but the Lucite bench top unit with a carbon filter.? I want to avoid the capital equipment rigmarole, so it has to cost less than $1000.? It is to put a small automated coverslipper under,? 16" H x 20" W x 10" D . ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???Thanks, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 29 Jun 2010 08:53:32 -0700 From: "Morken, Tim" Subject: RE: [Histonet] New CAP question ANP.22760 To: "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4C021C3610F1@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Thomas and Daniel both make good points. While clinical labs do run measurable (quantitative) tests they are also running completely closed systems in which all reagents are from a certain vendor. All the reagents are validated by a vendor and all reagents are validated and calibrated for that system. Running qualitative tests is only different at the interpretive stage. All other stages should be standardized and validated to ensure the system works as intended every day, every test. IHC, of course, is a whole different ballgame. There probably is not a single lab out there that uses only one vendor and uses only one system to do IHC testing. Even if you use one automated staining system you may use antibodies from a different vendor, or you may do something outside the system that is very different than other labs use. There is such disparity between testing in different labs that it is difficult to compare results and even techniques between labs. That is the crux of the issue and what CAP and others are trying to address by tightening the validation screws. In the near future labs will need to have very robust validation and documentation for all antibodies in order to prove they are doing things correctly. That scenario is already here for the breast markers. It will come for the others as well. Right now the instances in which solid validation methods are absolutely necessary is when validating the breast markers ER, PR and HER2, which all are used as stand-alone tests to determine treatment, and for ASR's. If you use a vendor kit with all reagents supplied you must still prove it works in your lab as intended ("verify outside data." The "outside data" is the claim that it stains in a certain way). You must run cases of various expressions and show your lab gets the proper results. Third party verification is very helpful, that is, comparing your results to that of other labs on the same material (CAP surveys, or set up a partnership with another lab). If you use only the antibody for one of those markers, or mix and match components outside a FDA-approved kit, then you are responsible for performing a comprehensive validation of the test. Its worth noting that every news article I've seen about pathology/histology labs in the last several years has been about failures to do the breast panel tests correctly. We are under a microscope these days and it will pay off to make sure you are doing the tests well! Most other markers in IHC are ancillary tests that are run in conjunction with other tests to determine a diagnosis. Those antibodies still need to be validated in your system but don't require a large sample of cases. They have been validated as IVD's by the vendor so just need to be shown they work as intended with your lab's methods and instruments. But I still think it is worthwhile to do a validation that includes a range of expressions along with irrelevant tissue (negative). That will help calibrate your expectations about how the antibody works and give you a baseline to refer to if problems arise. Running one or two slides to make sure it "works" is not really satisfactory. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, June 26, 2010 1:45 PM To: Daniel Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Daniel, I think your observations are very good. Which brings me again to a point I was trying to make earlier. Anatomic Pathology is not the same as the General Clinical Lab or it's close counterparts. It almost seems like this is overblown re: validation. As you've pointed out, the interpretation is up to the pathologist. Patients (prostate, i.e.) will correlate with other tests. When known positives demonstrate properly you're valid. Even automated systems can use algorithms to score strength of positivity...which varies yet will show as positive (valid). You receive a new batch of Ab you run it on a known positive, that's the bottom line. It seems somehow CAP or clinical lab (trained) folks want more than that. And again (as you pointed out) the appreciation for the differences in how we operate as laboratorians seems to fall by the wayside. As you mention pushing 20, 50, 100 is not feasible (and frankly unnecessary). I don't know where the answer lies in making everyone happy here. I do know that some thinking outside the box and trying understand AP is required. We all want good patient care, but one size does not fit all. Thanks, tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Sent: Saturday, June 26, 2010 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 One question I think a lot of you are not considering is that the clinical laboratory usually tests for analytes present in most patients. This allows the clinical lab to more easily run validations with hundreds of patients, using statistical tools to analyze the precision, specificity and sensitivity of the test. What most of those concerned with this new CAP standard (and I count among them) is that our testing often targets a very rare population of patients. As such, an extensive validation is much more difficult to establish. It then makes the statistical models used in clinical tests much less valuble since the accuracy of these formulas decrease with smaller sample sizes. Additionally, the pathology of the clinical tests are reported as a measurement which does not have an intrinsic value. It must be interpreted by a clinician to give value to the patient. For example, the Blood Urea Nitrogen test value range usually is between 7-20 mg/dL while values above 50 are considered a marker of poor kidney function. Elevated values, however, do not mean that kidney functions are impaired but instead may be due to other pathologies such as congestive heart failure, gi bleeding, certain steroids and even diet. For histopathology even relatively common antibodies such as pan-T cell marker CD3 usually only stain 75% of T cell neoplasms. A negative result for that minority does not indicate that the tumor does not have a T cell lineage. It relies instead on the pathologist interpreting the result much as the clinician looks at the BUN value in relation to other factors in the patient's presentation. When we validate this kind of antibody, do we perform testing then on T cells and B cells (give me a good tonsil), on malignant samples or some combination of the two? What is a reasonable number of tests to run then? If I choose 10 normal lymphatic tissue blocks for my routine T/B cell marking (it can't be as extensive as ER/PR can it?) how many samples should I choose for my malignant population? If I use another 10 anyone with a background in statistics will tell you that the sample size is too small to interpret accuracy. If I push it up to 20, 50 or 100 I would be certain that many laboratories would not be able to afford the cost of the validation. This would then push many good laboratories out of the business of IHC with the unintended result of delaying diagnoses and increasing patient costs by driving testing to fewer and fewer testing outlets. CAP's new path with ER/PR seems to be trying to achieve a noble end of improving quality in the laboratory but without an understanding of the complexities and consequences of the method they are implementing. Dan -----Original Message----- >From: Jesus Ellin >Sent: Jun 26, 2010 6:28 AM >To: "Morken, Tim" , >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >I have been reading the post to this question and it seems to me that there are different standards depending on the lab that is operating the methodology. I do agree that the core lab for years have had the instruction and training in the performance of validation. One thing that comes to mind as well is why has histology not had this training? Why are we not getting this from our certification agency, our professional societies and biggest reason where is our standardization. It seems to me that with all these regualtions in plac for so long, why were we missed. Is it because when inspected through CAP we are being inspected by a pathologist rather than a histo tech? These are some of the questions at hand. I to see new standards within the CAP checklist as well as other regulatory organizations that will affect the future of the Anatomic Pathology community. But I think we need is to provide a underlying architecture for our peers, so that we can begin the transition to the future. This is only the beginning, there is still Digital Image Analysis and Telepathology. It funny we are looking to become a hybrid of radiology and the core lab, but with the best of both worlds. Tim great structure for the validation study. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, >Tim >Sent: Wed 6/23/2010 9:48 AM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Joe, > >You wrote : The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time ..." > >Those were my thoughts exactly. While the person replying may or may not have specific histology experience she will have clinical lab experience (however, my guess is that she is exposed to histology regularly at CAP). Clinical labs have a bit of an easier time, actually, because they validate primarily to known concentration controls - analytical controls manufactured at a range of known concentrations for instance. The institution then adds in their normal controls for validation. > >As far as the current question about validating a new lot of reagent the best practice is to run parallel tests on the same machine. If that is not easily possible on a particular manufacturer's instrument then the question should be asked of them: Why not? If this is a requirement the manufacturer should provide an easy path to meeting the requirement. However, if that is not the case then the institution simply writes a procedure to get around the inadequacies of the instrument (Maybe the vendor can help with that). Then follow the procedure. That should satisfy inspectors. > >An "...appropriate panel of tissues..." is whatever the institution deems appropriate for the given antibody or reagent. This is a perfect place for tissue arrays. You can make your own or buy them. > >IHC must meet CLIA validation guidelines but since IHC is generally qualitative the requirements must be understood and methods adapted to a qualitative scenario. Several IHC and Histotechnology books discuss the subject at length (Taylor, Dabbs, Bancroft for instance). > >Below is a brief overview of how to do that. (for more in-depth info this was covered in an NSH teleconference I gave last year - PowerPoint, audio and references available from NSH-, and will be covered in a similar workshop at NSH in Seattle this year). > > >1) >CAP General Validation >CAP GEN.42020-42163 Test Method Validation Follows CLIA CFR Sec >493.1253 Does not apply well to IHC (IHC is usually qualitative) > >But the general principle applies: >The laboratory must have data on each test's accuracy, precision, analytic sensitivity, interferences and reportable range. > >Unmodified FDA-cleared or approved tests: the lab may use manufacturer information or published reports but lab must verify outside data. > >Non-FDA cleared: Lab MUST verify or establish analytic accuracy, precision, sensitivity, specificity and reportable range. > >2) Validation includes: >Accuracy: > Compare results with New antibody to a previously validated antibody on the same tissues > >Precision: > Test samples with varying antigen expression > Intra-run, Inter-run tests, 10 slides each (reproducibility) > >Sensitivity: > True Positive vs False Negative (higher % FN = less sensitive) > >Interferences [Specificity]: > True Negative vs False Positive (Higher % FP = less specific) > Delineate what could interfere to give a false positive or false negative result. > >Reportable Range > Establish a scoring system > Provide the definition of a positive result > >3)Sensitivity > >Analytic Sensitivity: > Lowest amount of substance detectable by the test > Can only be done with controls of known concentration > >Diagnostic Sensitivity: > Ability of the test to determine true diagnostic positive verses false negative (higher % FN = less sensitive) > Requires comparison to a previously validated antibody > >IHC Sensitivity: > Extent to which an antibody can be diluted and still achieve target recognition. NOTE: This is determined by antibody AND detection system! > > > >4) Specificity: > >Analytic Specificity > Accuracy on tests of known positive and negative controls > Controls of known concentration > Determine what could "Interfere" to confound the result > > >Diagnostic Specificity > Ability of a test to determine true diagnostic negative verses false positives (Higher % FP = less specific) > Requires comparison to a previously validated antibody > > >IHC Specificity > Ability of an antibody to bind exclusively to its particular antigen in the absence of staining of other molecules > Or, staining of other structures in addition to target >structures/cells > >(Sensitivity and Specificity adapted from: Theoretical and Practical >Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote, 2005) > >Tim Morken >Supervisor, Histology / IPOX >UCSF Medical Center >San Francisco, CA > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >JMyers1@aol.com >Sent: Tuesday, June 22, 2010 6:51 PM >To: tjasper@copc.net >Cc: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Tom: > >As much as I agree with your acknowledgment that its seems a bit odd >for the CAP to have a blood-banker responding to AP-related issue, I'm >actually not surprised. The folks in the 'clinical' lab have been >performing more comprehensive and complex validation procedures for a >very long time, and they wonder why IHC isn't expected to follow the >same requirements as chemistry, immunology, etc. -- IHC is, after all, >an awful lot like ELISA. And rightfully so, because IHC is, under CLIA >(which supersedes CAP), considered highly-complex, non-waived testing >-- and is, therefore, subject to the same Quality Systems regulations >(in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. > >Could it be that, because AP produces qualitative results that are >interpreted by a pathologist and CP produces quantitative results that >are interpreted by an analyzer, we somehow think that CLIA rules don't >apply to IHC? I certainly don't have the answer to that, but it make >me wonder what the future holds. As witnessed by some of the newest >CAP 'standards' (including the question in question...no pun intended), >e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens >must be tested, and where 10 of the positives must be weakly positive >-- an acknowledgment that validation specimens must be carefully >selected in order to obtain appropriate results), it certainly doesn't >appear that the regulation of IHC testing is going to become more relaxed. > >Joe Myers, M.S., CT(ASCP) > >------------------------------ > >Message: 12 >Date: Fri, 18 Jun 2010 12:38:07 -0700 >From: "Thomas Jasper" >Subject: RE: [Histonet] New CAP question ANP.22760 >To: "Mark Tarango" >Cc: _histonet@lists.utsouthwestern.edu_ >(mailto:histonet@lists.utsouthwestern.edu) > >Mark, > >Did you notice the credentials from this CAP representative? MT with a >Blood Bank specialty I believe. What I glean from that is...more than >likely this person does not grasp the logistics of "contemporaneously" >staining identical Abs from separate lots. She also likely does not >understand the logistical application for detection and automation >either. > >I'm not trying to be overly critical of this person. I'm sure she is >quite intelligent and would not have the MT/SBB if she wasn't >intelligent. It comes down to a lack of understanding Anatomic >Pathology testing application re: automated IHC. I believe this is a >common problem in and out of CAP. Many lab directors and other folks in >positions of authority without AP/Histology/Cytology backgrounds seem >to believe that broad clinical lab modalities apply to Anatomic Path >scenarios. I used to refer to this in my former position as - "Trying >to put the yoke of clinical lab onto anatomic path." We are >laboratorians, but in many instances do not fit the general clinical >lab mold. > >It's unfortunate that CAP has put this person in the position to >respond. It is apparent to me that she's not grasping the particulars >here. She probably never will unless she decides to go into a working, >automated IHC "tissue" lab and take the time to ask questions and >understand (learn) what we're all about. > >Thanks, >Tom Jasper > >Thomas Jasper HT (ASCP) BAS >Histology Supervisor >Central Oregon Regional Pathology Services Bend, OR 97701 >_______________________________________________ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 37 **************************************** ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* From sgoebel <@t> xbiotech.com Tue Jun 29 12:50:34 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Jun 29 12:50:39 2010 Subject: [Histonet] Fisher polymer kit Message-ID: <20100629105034.9e2d9aa830e8449a2412eb1e4f2f067e.f67473be6a.wbe@email04.secureserver.net> Hey histo hotties, Has anybody ever used the Thermo pol (Ultravision)? I have a rep who is trying very har me, but I have been a Dako girl for years. What iss had? Does it produce background? Dako is becomin for our company and with the help of this sales rep. the th stuff is being kind of pushed on me. I'm running a test right no just wondered if there were any other opinions out there. Than Sarah Goebel, B.A., HT (ASCP) Histotechnician < XBiotech USA Inc. < Austin, Texas 78744 < #3366ff;">( From alyssa <@t> alliedsearchpartners.com Tue Jun 29 13:27:28 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Jun 29 13:27:33 2010 Subject: [Histonet] Histotech Needed In Baton Rouge Message-ID: Our client, located in Baton Rouge, LA has retained Allied Search Partners and MPath Search Partners to take them through the search process for a highly qualified Histotechnician or Histotechnologist. This is a unique opportunity to join a family oriented team with flexible works hours. Allied Search Partners is currently accepting resumes in the Baton Rouge, LA area for qualified Histotechnicians/Histotechnologists. This is a PERMANENT position. No Contractors/Travelers please. Position Title: Histotechnologist/Histotechnician Job Description: Daily routine histology. Shifts: Day Shift, 6am-2:30pm. Monday-Friday. May vary by an hour or so at times. Location: Private Lab Benefits: Fully benefited with Medical Insurance, Paid Time Off, Relocation Assistance, and more to be discussed with the HR manager if phone interview is requested. Requirements: At least 3-5 years experience and HTL or HT ASCP certified Please submit your resume for prescreening purposes to alyssa@alliedsearchpartners.com *All inquiries are always kept confidential* upon resume submission one of our recruiters will call you for an initial phone interview. No resume will be submitted before an initial phone interview. Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for a generous bonus, and have your resume reviewed for free by our career advisors. -- Alyssa Peterson, Director of Candidate Recruitment Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From rjbuesa <@t> yahoo.com Tue Jun 29 14:17:14 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 29 14:17:18 2010 Subject: [Histonet] Canine bone sectioning trouble In-Reply-To: <94A0BFCE84CB0F4A84C50EAC9B02D0A9019423208F@HSC-CMS01.ad.ufl.edu> Message-ID: <562859.8016.qm@web65715.mail.ac4.yahoo.com> Your problem is the decalcification, compounded in the case of teeth by the enamel. Address both issues and you will be able to section them. Ren? J. --- On Tue, 6/29/10, Schneider,Lynda S wrote: From: Schneider,Lynda S Subject: [Histonet] Canine bone sectioning trouble To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, June 29, 2010, 12:17 PM Hello out there... We are having trouble sectioning canine bone.? The samples are bone marrow cubes approximately? 2cm thick and 1in wide.? They were fixed for 15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid decalcifier.? They were faced in and then surface decaled again for about 30 mins.? When sectioned, much of the marrow was missing and the bone was torn and shredded.? We thought that maybe the samples had not been fixed sufficiently so refixed overnight in 10% NBF again.? The samples were then reprocessed as they had been originally.? The processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each, paraffin x 4, 40 mins each. Again they were faced in and decaled for another 30 mins or so.? This time as soon as the sections are placed on the water bath (38 degrees) they explode and/or come apart so severely sections can almost not be picked up.? If sections are even obtainable they are of horrible quality.? Does anyone have any suggestions? Thank you so much in advance! Lynda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brian <@t> prometheushealthcare.com Tue Jun 29 19:51:32 2010 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Tue Jun 29 19:51:36 2010 Subject: [Histonet] Histotech Opening in Baton Rouge, LA Message-ID: <005001cb17ee$620c2710$26247530$@com> looking for a certified histo tech with at least 3-5 years experience to fill a morning position (embedding, cutting, special stains and fluid cytology) in a private histology lab in Baton Rouge, Louisiana Contact me today for immediate consideration Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian @prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From AToothman <@t> ParmaHospital.org Wed Jun 30 06:05:41 2010 From: AToothman <@t> ParmaHospital.org (AToothman@ParmaHospital.org) Date: Wed Jun 30 06:03:44 2010 Subject: [Histonet] RE: Histonet Digest, Vol 79, Issue 38 In-Reply-To: <201006291724.o5THOOep003655@node0.securemail.parmahospital.org> Message-ID: <3D6A6396C010F9448CDA1790C5DAA21001145345@pcghexchange1.pcgh.local> For Malika I have the Leica Coverslipper and I use Statlab coverslips. They work great and almost no waste. Hope this helps you ... Audrey Toothman Parma Community General Hospital Parma Ohio -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, June 29, 2010 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Canine bone sectioning trouble (Schneider,Lynda S) 2. RE: Coverslips (Malika Benatti) ---------------------------------------------------------------------- Message: 1 Date: Tue, 29 Jun 2010 12:17:58 -0400 From: "Schneider,Lynda S" Subject: [Histonet] Canine bone sectioning trouble To: "histonet@lists.utsouthwestern.edu" Message-ID: <94A0BFCE84CB0F4A84C50EAC9B02D0A9019423208F@HSC-CMS01.ad.ufl.edu> Content-Type: text/plain; charset="us-ascii" Hello out there... We are having trouble sectioning canine bone. The samples are bone marrow cubes approximately 2cm thick and 1in wide. They were fixed for 15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid decalcifier. They were faced in and then surface decaled again for about 30 mins. When sectioned, much of the marrow was missing and the bone was torn and shredded. We thought that maybe the samples had not been fixed sufficiently so refixed overnight in 10% NBF again. The samples were then reprocessed as they had been originally. The processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each, paraffin x 4, 40 mins each. Again they were faced in and decaled for another 30 mins or so. This time as soon as the sections are placed on the water bath (38 degrees) they explode and/or come apart so severely sections can almost not be picked up. If sections are even obtainable they are of hor! rible quality. Does anyone have any suggestions? Thank you so much in advance! Lynda ------------------------------ Message: 2 Date: Tue, 29 Jun 2010 17:53:26 +0100 From: "Malika Benatti" Subject: [Histonet] RE: Coverslips To: Message-ID: <4C2A3319.4626.0038.0@gosh.nhs.uk> Content-Type: text/plain; charset=UTF-8 ** Proprietary ** ** Reply Requested When Convenient ** Hi there, We use the CellPath CoverSlips 22x50mm No 1.5 they are great for hand mounting but when used with the Leica CV5030 they are a real pain. most of the CoversSlips get rejected. If anyone use the same equipment, let me know how you are getting one . Cheers, Malika ------------------------------ Message: 7 Date: Mon, 28 Jun 2010 15:06:08 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: Coverslips To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C93@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 8 Date: Mon, 28 Jun 2010 12:21:33 -0700 From: "Kathleen Boozer" Subject: Re: [Histonet] Coverslips To: "histonet@lists.utsouthwestern.edu" , "Joyce Weems" Message-ID: <4C2893CC.4AA8.00C0.1@ah.org> Content-Type: text/plain; charset=US-ASCII I am having the same issues and we purchased the expensive "Platinum" to avoid that problem. They just sent me replacements and I haven't tested them all yet. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk >>> "Weems, Joyce" 6/28/2010 11:28 AM >>> For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 28 Jun 2010 15:36:22 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] RE: Coverslips To: "Weems, Joyce" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2441E@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" We have been using them (24x50) and have had no problems with broken glass pieces. I do agree that they seem "dirty". Never contacted them about it. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 3:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Coverslips I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 10 Date: Mon, 28 Jun 2010 12:41:44 -0700 From: sgoebel@xbiotech.com Subject: RE: [Histonet] RE: Coverslips To: "Weems,Joyce" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <20100628124144.9e2d9aa830e8449a2412eb1e4f2f067e.fdd3de83db.wbe@email04.secureserver.net> Content-Type: text/plain; charset="utf-8" I just got a box of these as a "trial". They all have been far (used 1/2 the box so far)? Maybe there is gunk in your co verslipper that is causing this or possibly some glass particles? I c Sarah Goebel, B.A., HT (ASCP) < Histotechnicia XBiotech USA Inc. < Austin, Texas 78744 < (512) -------- Original Message -------- Subject: [Histonet] RE: Coverslips From: "Weems, Joyce" <[1]JWeems@sjha.o Date: Mon, June 28, 2010 12:06 pm To: "[2]histonet@lists. <[3]histonet@lists.u I forgot to say that the packaging has changed to their Histo Hippo theme, -----Original Message----- From: [4]histon [[5]mailto:histonet-bounces@lists.utsouthwestern.edu Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: [6]histonet@lists.u Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white with them air bubbles when us having this problem? They and have replaced several boxes, same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health E recipient(s). It may contain information that is privileged and confidential. Any unauth If you are n and reply to the sen _______________________________________________ Histonet mailing list [7]Histonet@lists.utsou [8]http: Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list [9]Histonet@lists.utsou [10]http: References 1. 3D"mailto://JWeems@sjha.org"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 4. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 5. 3D"mailto:histonet-bounces 6. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 7. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 8. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 9. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" ------------------------------ Message: 11 Date: Mon, 28 Jun 2010 14:48:44 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] Tissue Processors To: "'Feher, Stephen'" , mohamed abd el razik , "Histonet@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2CC@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="iso-8859-1" I second Steve's comments about the Peloris. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, June 28, 2010 1:56 PM To: mohamed abd el razik; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processors We have 2 Peloris processors and run protocols ranging from 2 hrs to overnight. Several 2 and 4 hour protocols daily keeps a constant flow of specimens moving through the lab for those interested in LEAN small batch processing. As far as programmability, it 's all touch screen driven and easy. It's a smart processor so we don't change all solutions weekly. We only change the ones that are needed. The parameters that determine how long to use a particular solution are also programmable. Changing solutions is done by pumping out of the individual bottles and into waste containers and refilling is pumped from clean containers of solution back into the original containers. No heavy lifting required. Ours are in a separate room from our microtomy area so we hooked a simple door bell up to the local alarm jack on the back. When processing is done, we get the appropriate tone. Remote alarm is also tied in to the hospital switchboard in case a processor goes down at night or during the weekend. We really like the versatility and dependability of these processors. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Saturday, June 26, 2010 3:41 PM To: Histonet@lists.utsouthwestern.edu Subject: Fw: [Histonet] Tissue Processors i need it too as we are going to bring new tissue processor to our small lab --- On Sat, 6/26/10, Shirley Pan wrote: From: Shirley Pan Subject: [Histonet] Tissue Processors To: histonet@lists.utsouthwestern.edu Date: Saturday, June 26, 2010, 7:55 AM We are in the process of trying out tissue processors. Are there any users of the Leica Peloris or Thermo EG who can help us out with some opinions? Reliability, ease of changing solutions, programmability? Thanks for any help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. 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Thank you for your cooperation. ------------------------------ Message: 12 Date: Mon, 28 Jun 2010 16:04:27 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] RE: Coverslips To: "sgoebel@xbiotech.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5CBA@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We can coverslip by hand, but soon after the slides are coverslipped on the machine, air bubbles begin to form. We we coverslip them by hand, there is visible glass that we can feel and move if we need too. They've been good for so long, I want to make sure it's not just us. Thanks for the feedback! j ________________________________ From: sgoebel@xbiotech.com [mailto:sgoebel@xbiotech.com] Sent: Monday, June 28, 2010 15:42 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Coverslips I just got a box of these as a "trial". They all have been fine so far (used 1/2 the box so far)? Maybe there is gunk in your coverslipper that is causing this or possibly some glass particles? I coverslip by hand. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] RE: Coverslips From: "Weems, Joyce" > Date: Mon, June 28, 2010 12:06 pm To: "histonet@lists.utsouthwestern.edu" > I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 13 Date: Mon, 28 Jun 2010 13:19:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Unstained Slides To: histonet@lists.utsouthwestern.edu, Rick.Garnhart@memorialhealthsystem.com Message-ID: <946724.39272.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Depending on the??epitope you are trying to detect, the time could be limited??from less than one month to 1-2 years. Ren?~ J. --- On Mon, 6/28/10, Rick.Garnhart@memorialhealthsystem.com wrote: From: Rick.Garnhart@memorialhealthsystem.com Subject: [Histonet] Unstained Slides To: histonet@lists.utsouthwestern.edu Date: Monday, June 28, 2010, 11:54 AM Histoland, How?? is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph:?? 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 28 Jun 2010 14:58:48 -0600 From: Jay Lundgren Subject: [Histonet] Inexpensive fume hood? To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Can anyone recommend an inexpensive fume hood/ workstation? Not the full on metal type that hangs from the ceiling, but the Lucite bench top unit with a carbon filter. I want to avoid the capital equipment rigmarole, so it has to cost less than $1000. It is to put a small automated coverslipper under, 16" H x 20" W x 10" D . Thanks, Jay A. Lundgren, M.S., HTL (ASCP) ------------------------------ Message: 15 Date: Mon, 28 Jun 2010 14:04:42 -0700 From: Pat Laurie Subject: Re: [Histonet] Tissue Processors To: "Mahoney,Janice A" Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I 3rd the previous statements about the Peloris. I had 2 pelori at my previous job, and now we currently have 3 pelori. We are also looking at getting a 4th. We use them regularly and due to the ability to use shorter programs, we run about 15 runs a day between them while still having significant downtime. I must warn you though, it is necessary to keep the service contracts on them. They are about 99% computer and have a significant number of moving parts, but they do work wonderfully. On Mon, Jun 28, 2010 at 12:48 PM, Mahoney,Janice A < Janice.Mahoney@alegent.org> wrote: > I second Steve's comments about the Peloris. > Jan Mahoney > Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen > Sent: Monday, June 28, 2010 1:56 PM > To: mohamed abd el razik; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Tissue Processors > > We have 2 Peloris processors and run protocols ranging from 2 hrs to > overnight. Several 2 and 4 hour protocols daily keeps a constant flow of > specimens moving through the lab for those interested in LEAN small batch > processing. As far as programmability, it 's all touch screen driven and > easy. It's a smart processor so we don't change all solutions weekly. We > only change the ones that are needed. The parameters that determine how > long to use a particular solution are also programmable. Changing solutions > is done by pumping out of the individual bottles and into waste containers > and refilling is pumped from clean containers of solution back into the > original containers. No heavy lifting required. > > Ours are in a separate room from our microtomy area so we hooked a simple > door bell up to the local alarm jack on the back. When processing is done, > we get the appropriate tone. Remote alarm is also tied in to the hospital > switchboard in case a processor goes down at night or during the weekend. > > We really like the versatility and dependability of these processors. > > > Steve > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el > razik > Sent: Saturday, June 26, 2010 3:41 PM > To: Histonet@lists.utsouthwestern.edu > Subject: Fw: [Histonet] Tissue Processors > > > i need it too as we are going to bring new tissue processor to our small > lab > --- On Sat, 6/26/10, Shirley Pan wrote: > > > From: Shirley Pan > Subject: [Histonet] Tissue Processors > To: histonet@lists.utsouthwestern.edu > Date: Saturday, June 26, 2010, 7:55 AM > > > We are in the process of trying out tissue processors. Are there any users > of the Leica Peloris or Thermo EG who can help us out with some opinions? > Reliability, ease of changing solutions, programmability? Thanks for any > help. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is > faithful to the healing ministry of Jesus Christ, providing high quality > care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is > confidential and private and intended only for the use of the addressees. > Unauthorized use, disclosure, distribution or copying is strictly > prohibited and may be unlawful. If you received this communication in > error, please inform us of the erroneous delivery by return e-mail message > from your computer. Additionally, although all attachments have been > scanned at the source for viruses, the recipient should check any > attachments for the presence of viruses before opening. Alegent Health > accepts no liability for any damage caused by any virus transmitted by this > e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com ------------------------------ Message: 16 Date: Mon, 28 Jun 2010 17:57:37 -0500 From: "Amador, Amanda" Subject: [Histonet] Modified Genta To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <1D9B439FC446244095353DCA4FC23DD20454E1D27B@MWNMAIL00.ameripath.local> Content-Type: text/plain; charset="us-ascii" Is there someone that could help out with the Modified Genta? We tried it in the microwave and we are not sure if we are doing it correctly. We would appreciate any sample procedures to see if we need to change something. Thanks to anyone who can help out. aamador@ameripath.com ------------------------------ Message: 17 Date: Tue, 29 Jun 2010 09:53:10 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] what do you think? To: mohamed abd el razik Cc: Histonet@lists.utsouthwestern.edu Message-ID: <4C29FAC6.1030909@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Greetings Mohamed: The lamina propria brings small blood vessels and lymphatics close to the epithelium lining the large and small intestines. This allows nutrients to be passed back and forth and for the immune system to monitor any antigens that cross the epithelium. The relationship between the immune system and the contents of the GI tract is very complex. Lymph nodules in the lamina propria would make it thicker in some regions than in other regions. Whether the thickening you refer to is pathological or just the normal response to a variety of factors is difficult to say. Geoff mohamed abd el razik wrote: > dear histonetters > i have a quistion please regarding the thickness of lamina propria in small or larg intestine. > what is the significant of it?? does it mean inflamation and pathological condition or mean more size and so absorption of nutrients? i mean does the thickning is favorable or not? > > thanks > Mohamed Abd Elrazik > Histology dep. > Fac. of Vet. Med. > Cairo . Univ. -Egypt > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 18 Date: 29 Jun 2010 13:59:09 -0000 From: "abijag " Subject: [Histonet] Your experience with microm HMS 740 auto stainer To: Message-ID: <1277819686.S.1418.34634.webmail.rediff.com.drafts.1277819949.50235@webmail.rediffmail.com> Content-Type: text/plain; charset="UTF-8" Dear Histonetters, We would like to procure one tissue auto stainer for our histology lab(mainly H&E staining) In this respect, I request your experience about Microm HMS 740 automatic stainer. Please share your comments regarding the staining reproducibility,ease of handling and their claim about drip prevention technology. Based on your valuable feedback, we will make our decision. Thanks for all help Abi jagannath ------------------------------ Message: 19 Date: Tue, 29 Jun 2010 08:20:32 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Inexpensive fume hood? To: histonet , Jay Lundgren Message-ID: <364541.78070.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Check a Fisher catalog. The ones they sell are good for your purposes. Ren?~ J. --- On Mon, 6/28/10, Jay Lundgren wrote: From: Jay Lundgren Subject: [Histonet] Inexpensive fume hood? To: "histonet" Date: Monday, June 28, 2010, 4:58 PM Can anyone recommend an inexpensive fume hood/ workstation??? Not the full on metal type that hangs from the ceiling, but the Lucite bench top unit with a carbon filter.?? I want to avoid the capital equipment rigmarole, so it has to cost less than $1000.?? It is to put a small automated coverslipper under,?? 16" H x 20" W x 10" D . ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ??????Thanks, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 29 Jun 2010 08:53:32 -0700 From: "Morken, Tim" Subject: RE: [Histonet] New CAP question ANP.22760 To: "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4C021C3610F1@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Thomas and Daniel both make good points. While clinical labs do run measurable (quantitative) tests they are also running completely closed systems in which all reagents are from a certain vendor. All the reagents are validated by a vendor and all reagents are validated and calibrated for that system. Running qualitative tests is only different at the interpretive stage. All other stages should be standardized and validated to ensure the system works as intended every day, every test. IHC, of course, is a whole different ballgame. There probably is not a single lab out there that uses only one vendor and uses only one system to do IHC testing. Even if you use one automated staining system you may use antibodies from a different vendor, or you may do something outside the system that is very different than other labs use. There is such disparity between testing in different labs that it is difficult to compare results and even techniques between labs. That is the crux of the issue and what CAP and others are trying to address by tightening the validation screws. In the near future labs will need to have very robust validation and documentation for all antibodies in order to prove they are doing things correctly. That scenario is already here for the breast markers. It will come for the others as well. Right now the instances in which solid validation methods are absolutely necessary is when validating the breast markers ER, PR and HER2, which all are used as stand-alone tests to determine treatment, and for ASR's. If you use a vendor kit with all reagents supplied you must still prove it works in your lab as intended ("verify outside data." The "outside data" is the claim that it stains in a certain way). You must run cases of various expressions and show your lab gets the proper results. Third party verification is very helpful, that is, comparing your results to that of other labs on the same material (CAP surveys, or set up a partnership with another lab). If you use only the antibody for one of those markers, or mix and match components outside a FDA-approved kit, then you are responsible for performing a comprehensive validation of the test. Its worth noting that every news article I've seen about pathology/histology labs in the last several years has been about failures to do the breast panel tests correctly. We are under a microscope these days and it will pay off to make sure you are doing the tests well! Most other markers in IHC are ancillary tests that are run in conjunction with other tests to determine a diagnosis. Those antibodies still need to be validated in your system but don't require a large sample of cases. They have been validated as IVD's by the vendor so just need to be shown they work as intended with your lab's methods and instruments. But I still think it is worthwhile to do a validation that includes a range of expressions along with irrelevant tissue (negative). That will help calibrate your expectations about how the antibody works and give you a baseline to refer to if problems arise. Running one or two slides to make sure it "works" is not really satisfactory. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, June 26, 2010 1:45 PM To: Daniel Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Daniel, I think your observations are very good. Which brings me again to a point I was trying to make earlier. Anatomic Pathology is not the same as the General Clinical Lab or it's close counterparts. It almost seems like this is overblown re: validation. As you've pointed out, the interpretation is up to the pathologist. Patients (prostate, i.e.) will correlate with other tests. When known positives demonstrate properly you're valid. Even automated systems can use algorithms to score strength of positivity...which varies yet will show as positive (valid). You receive a new batch of Ab you run it on a known positive, that's the bottom line. It seems somehow CAP or clinical lab (trained) folks want more than that. And again (as you pointed out) the appreciation for the differences in how we operate as laboratorians seems to fall by the wayside. As you mention pushing 20, 50, 100 is not feasible (and frankly unnecessary). I don't know where the answer lies in making everyone happy here. I do know that some thinking outside the box and trying understand AP is required. We all want good patient care, but one size does not fit all. Thanks, tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Sent: Saturday, June 26, 2010 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 One question I think a lot of you are not considering is that the clinical laboratory usually tests for analytes present in most patients. This allows the clinical lab to more easily run validations with hundreds of patients, using statistical tools to analyze the precision, specificity and sensitivity of the test. What most of those concerned with this new CAP standard (and I count among them) is that our testing often targets a very rare population of patients. As such, an extensive validation is much more difficult to establish. It then makes the statistical models used in clinical tests much less valuble since the accuracy of these formulas decrease with smaller sample sizes. Additionally, the pathology of the clinical tests are reported as a measurement which does not have an intrinsic value. It must be interpreted by a clinician to give value to the patient. For example, the Blood Urea Nitrogen test value range usually is between 7-20 mg/dL while values above 50 are considered a marker of poor kidney function. Elevated values, however, do not mean that kidney functions are impaired but instead may be due to other pathologies such as congestive heart failure, gi bleeding, certain steroids and even diet. For histopathology even relatively common antibodies such as pan-T cell marker CD3 usually only stain 75% of T cell neoplasms. A negative result for that minority does not indicate that the tumor does not have a T cell lineage. It relies instead on the pathologist interpreting the result much as the clinician looks at the BUN value in relation to other factors in the patient's presentation. When we validate this kind of antibody, do we perform testing then on T cells and B cells (give me a good tonsil), on malignant samples or some combination of the two? What is a reasonable number of tests to run then? If I choose 10 normal lymphatic tissue blocks for my routine T/B cell marking (it can't be as extensive as ER/PR can it?) how many samples should I choose for my malignant population? If I use another 10 anyone with a background in statistics will tell you that the sample size is too small to interpret accuracy. If I push it up to 20, 50 or 100 I would be certain that many laboratories would not be able to afford the cost of the validation. This would then push many good laboratories out of the business of IHC with the unintended result of delaying diagnoses and increasing patient costs by driving testing to fewer and fewer testing outlets. CAP's new path with ER/PR seems to be trying to achieve a noble end of improving quality in the laboratory but without an understanding of the complexities and consequences of the method they are implementing. Dan -----Original Message----- >From: Jesus Ellin >Sent: Jun 26, 2010 6:28 AM >To: "Morken, Tim" , >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >I have been reading the post to this question and it seems to me that there are different standards depending on the lab that is operating the methodology. I do agree that the core lab for years have had the instruction and training in the performance of validation. One thing that comes to mind as well is why has histology not had this training? Why are we not getting this from our certification agency, our professional societies and biggest reason where is our standardization. It seems to me that with all these regualtions in plac for so long, why were we missed. Is it because when inspected through CAP we are being inspected by a pathologist rather than a histo tech? These are some of the questions at hand. I to see new standards within the CAP checklist as well as other regulatory organizations that will affect the future of the Anatomic Pathology community. But I think we need is to provide a underlying architecture for our peers, so that we can begin the transition to the future. This is only the beginning, there is still Digital Image Analysis and Telepathology. It funny we are looking to become a hybrid of radiology and the core lab, but with the best of both worlds. Tim great structure for the validation study. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, >Tim >Sent: Wed 6/23/2010 9:48 AM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Joe, > >You wrote : The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time ..." > >Those were my thoughts exactly. While the person replying may or may not have specific histology experience she will have clinical lab experience (however, my guess is that she is exposed to histology regularly at CAP). Clinical labs have a bit of an easier time, actually, because they validate primarily to known concentration controls - analytical controls manufactured at a range of known concentrations for instance. The institution then adds in their normal controls for validation. > >As far as the current question about validating a new lot of reagent the best practice is to run parallel tests on the same machine. If that is not easily possible on a particular manufacturer's instrument then the question should be asked of them: Why not? If this is a requirement the manufacturer should provide an easy path to meeting the requirement. However, if that is not the case then the institution simply writes a procedure to get around the inadequacies of the instrument (Maybe the vendor can help with that). Then follow the procedure. That should satisfy inspectors. > >An "...appropriate panel of tissues..." is whatever the institution deems appropriate for the given antibody or reagent. This is a perfect place for tissue arrays. You can make your own or buy them. > >IHC must meet CLIA validation guidelines but since IHC is generally qualitative the requirements must be understood and methods adapted to a qualitative scenario. Several IHC and Histotechnology books discuss the subject at length (Taylor, Dabbs, Bancroft for instance). > >Below is a brief overview of how to do that. (for more in-depth info this was covered in an NSH teleconference I gave last year - PowerPoint, audio and references available from NSH-, and will be covered in a similar workshop at NSH in Seattle this year). > > >1) >CAP General Validation >CAP GEN.42020-42163 Test Method Validation Follows CLIA CFR Sec >493.1253 Does not apply well to IHC (IHC is usually qualitative) > >But the general principle applies: >The laboratory must have data on each test's accuracy, precision, analytic sensitivity, interferences and reportable range. > >Unmodified FDA-cleared or approved tests: the lab may use manufacturer information or published reports but lab must verify outside data. > >Non-FDA cleared: Lab MUST verify or establish analytic accuracy, precision, sensitivity, specificity and reportable range. > >2) Validation includes: >Accuracy: > Compare results with New antibody to a previously validated antibody on the same tissues > >Precision: > Test samples with varying antigen expression > Intra-run, Inter-run tests, 10 slides each (reproducibility) > >Sensitivity: > True Positive vs False Negative (higher % FN = less sensitive) > >Interferences [Specificity]: > True Negative vs False Positive (Higher % FP = less specific) > Delineate what could interfere to give a false positive or false negative result. > >Reportable Range > Establish a scoring system > Provide the definition of a positive result > >3)Sensitivity > >Analytic Sensitivity: > Lowest amount of substance detectable by the test > Can only be done with controls of known concentration > >Diagnostic Sensitivity: > Ability of the test to determine true diagnostic positive verses false negative (higher % FN = less sensitive) > Requires comparison to a previously validated antibody > >IHC Sensitivity: > Extent to which an antibody can be diluted and still achieve target recognition. NOTE: This is determined by antibody AND detection system! > > > >4) Specificity: > >Analytic Specificity > Accuracy on tests of known positive and negative controls > Controls of known concentration > Determine what could "Interfere" to confound the result > > >Diagnostic Specificity > Ability of a test to determine true diagnostic negative verses false positives (Higher % FP = less specific) > Requires comparison to a previously validated antibody > > >IHC Specificity > Ability of an antibody to bind exclusively to its particular antigen in the absence of staining of other molecules > Or, staining of other structures in addition to target >structures/cells > >(Sensitivity and Specificity adapted from: Theoretical and Practical >Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote, 2005) > >Tim Morken >Supervisor, Histology / IPOX >UCSF Medical Center >San Francisco, CA > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >JMyers1@aol.com >Sent: Tuesday, June 22, 2010 6:51 PM >To: tjasper@copc.net >Cc: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Tom: > >As much as I agree with your acknowledgment that its seems a bit odd >for the CAP to have a blood-banker responding to AP-related issue, I'm >actually not surprised. The folks in the 'clinical' lab have been >performing more comprehensive and complex validation procedures for a >very long time, and they wonder why IHC isn't expected to follow the >same requirements as chemistry, immunology, etc. -- IHC is, after all, >an awful lot like ELISA. And rightfully so, because IHC is, under CLIA >(which supersedes CAP), considered highly-complex, non-waived testing >-- and is, therefore, subject to the same Quality Systems regulations >(in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. > >Could it be that, because AP produces qualitative results that are >interpreted by a pathologist and CP produces quantitative results that >are interpreted by an analyzer, we somehow think that CLIA rules don't >apply to IHC? I certainly don't have the answer to that, but it make >me wonder what the future holds. As witnessed by some of the newest >CAP 'standards' (including the question in question...no pun intended), >e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens >must be tested, and where 10 of the positives must be weakly positive >-- an acknowledgment that validation specimens must be carefully >selected in order to obtain appropriate results), it certainly doesn't >appear that the regulation of IHC testing is going to become more relaxed. > >Joe Myers, M.S., CT(ASCP) > >------------------------------ > >Message: 12 >Date: Fri, 18 Jun 2010 12:38:07 -0700 >From: "Thomas Jasper" >Subject: RE: [Histonet] New CAP question ANP.22760 >To: "Mark Tarango" >Cc: _histonet@lists.utsouthwestern.edu_ >(mailto:histonet@lists.utsouthwestern.edu) > >Mark, > >Did you notice the credentials from this CAP representative? MT with a >Blood Bank specialty I believe. What I glean from that is...more than >likely this person does not grasp the logistics of "contemporaneously" >staining identical Abs from separate lots. She also likely does not >understand the logistical application for detection and automation >either. > >I'm not trying to be overly critical of this person. I'm sure she is >quite intelligent and would not have the MT/SBB if she wasn't >intelligent. It comes down to a lack of understanding Anatomic >Pathology testing application re: automated IHC. I believe this is a >common problem in and out of CAP. Many lab directors and other folks in >positions of authority without AP/Histology/Cytology backgrounds seem >to believe that broad clinical lab modalities apply to Anatomic Path >scenarios. I used to refer to this in my former position as - "Trying >to put the yoke of clinical lab onto anatomic path." We are >laboratorians, but in many instances do not fit the general clinical >lab mold. > >It's unfortunate that CAP has put this person in the position to >respond. It is apparent to me that she's not grasping the particulars >here. She probably never will unless she decides to go into a working, >automated IHC "tissue" lab and take the time to ask questions and >understand (learn) what we're all about. > >Thanks, >Tom Jasper > >Thomas Jasper HT (ASCP) BAS >Histology Supervisor >Central Oregon Regional Pathology Services Bend, OR 97701 >_______________________________________________ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 37 **************************************** ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 38 **************************************** From settembr <@t> umdnj.edu Wed Jun 30 07:12:09 2010 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Jun 30 07:12:26 2010 Subject: [Histonet] Anybody running PIN 4 on Bond Message-ID: Is anybody running the PIN 4 cocktail on Leica's Bond Max on human tissue? We are trying to bring PIN 4 into our lab. I can't seem to get consistent staining. I am using BioCare's PIN 4 antibodies, Mach 2 and Leica's RED Refine detection kit. I have used Leica's recommended times. We have a great positive control that worked wonderfully when first optimized, so I thought this Leica protocol was great. But could not duplicate results. The RED has always been fine. I have had BioCare replace their antibody.(nuclear staining very light, if there) Have changed protocols times as per BioCare's Tech Services. I am at my wits end. Help. What is your protocol? My director really wants this up and running. Thank you, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA From Rcartun <@t> harthosp.org Wed Jun 30 08:43:08 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jun 30 08:43:17 2010 Subject: [Histonet] H&E slide cost Message-ID: <4C2B11AB.7400.0077.1@harthosp.org> I apologize for asking this question since I'm sure it's been discussed before, but I need an answer ASAP. How much does it cost to produce an H&E-stained slide from formalin-fixed tissue? We want to give this figure to our new residents and fellows who start tomorrow in order to show them how much it costs the laboratory to produce the tissue that they submit. Thanks! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jun 30 08:57:38 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Jun 30 08:57:43 2010 Subject: [Histonet] H&E stainers Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB2722@HPEMX3.HealthPartners.int> We are in the market for a new H&E automated stainer and am hoping I get some feedback on this request!!!!! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments "private" at Dorothy.L.Webb@Healthpartners.com Thanks much ahead of time!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From MSHERWOOD <@t> PARTNERS.ORG Wed Jun 30 09:35:19 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jun 30 09:35:34 2010 Subject: [Histonet] H&E stainers In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB2722@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB2722@HPEMX3.HealthPartners.int> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2442D@PHSXMB30.partners.org> We have a "refurbished" Leica Autostainer XL and are very happy with it. We are a research lab, so our volume is not as much as a hospital histology lab, but we get big projects every now and then and it has paid for itself. If you are interested in purchasing refurbished equipment, please contact me directly (msherwood@partners.org). Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, June 30, 2010 9:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] H&E stainers We are in the market for a new H&E automated stainer and am hoping I get some feedback on this request!!!!! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments "private" at Dorothy.L.Webb@Healthpartners.com Thanks much ahead of time!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From 41dmb41 <@t> gmail.com Wed Jun 30 09:41:56 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Wed Jun 30 09:42:30 2010 Subject: [Histonet] H. pylori RTU antibody Message-ID: I was just wondering if a lot of people use the RTU antibodies available out there for H. pylori. Currently, we're testing the RTU from Dako and we're having issues with a lot of background staining. After tweaking the protocol a lot, I've managed to get a lot of it removed, but there are still some issues. I was wondering if most people are making their own dilutions to get a better stain or if the RTUs are commonplace. I appreciate the feedback! Thanks, Drew From Lynn.Burton <@t> Illinois.gov Wed Jun 30 09:48:42 2010 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Jun 30 09:49:51 2010 Subject: [Histonet] FW: bovine viral diarhea antibody References: Message-ID: I am looking for a vendor of BVDV antibody for IHC testing. We had used Syracuse Bioanalytical in the past. They seem to be gone. I would appreciate any suggestions. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From k84as <@t> yahoo.com Wed Jun 30 10:05:46 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Wed Jun 30 10:05:51 2010 Subject: Fw: [Histonet] H&E stainers Message-ID: <256181.49899.qm@web112603.mail.gq1.yahoo.com> we are going to bring new one too for our small lab. any suggestion please. you can send me at k84as@yahoo.com --- On Wed, 6/30/10, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] H&E stainers To: "'histonet@lists.utsouthwestern.edu'" Date: Wednesday, June 30, 2010, 4:57 PM We are in the market for a new H&E automated stainer and am hoping I get some feedback on this request!!!!!? I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!!? You can email me directly if you would like your comments "private" at? Dorothy.L.Webb@Healthpartners.com Thanks much ahead of time!! ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Jun 30 10:05:25 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Jun 30 10:06:20 2010 Subject: [Histonet] H. pylori RTU antibody In-Reply-To: References: Message-ID: Use the RTU from Biocare. It is clean and easy. You can use it with the Dako Flex Kit. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer [41dmb41@gmail.com] Sent: Wednesday, June 30, 2010 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. pylori RTU antibody I was just wondering if a lot of people use the RTU antibodies available out there for H. pylori. Currently, we're testing the RTU from Dako and we're having issues with a lot of background staining. After tweaking the protocol a lot, I've managed to get a lot of it removed, but there are still some issues. I was wondering if most people are making their own dilutions to get a better stain or if the RTUs are commonplace. I appreciate the feedback! Thanks, Drew _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Wed Jun 30 10:33:29 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Jun 30 10:33:34 2010 Subject: [Histonet] FW: bovine viral diarhea antibody References: Message-ID: <58998E61F28D465CA02119D78C8D7C43@auxs.umn.edu> VMRD Mouse monoclonals - Clone D89 and clone BA-2 are now working stronger than my previous clone (15C5 from another vendor). Both need heat retrieval. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Burton, Lynn" To: Sent: Wednesday, June 30, 2010 9:48 AM Subject: [Histonet] FW: bovine viral diarhea antibody I am looking for a vendor of BVDV antibody for IHC testing. We had used Syracuse Bioanalytical in the past. They seem to be gone. I would appreciate any suggestions. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SEsparza <@t> seton.org Wed Jun 30 10:46:51 2010 From: SEsparza <@t> seton.org (Esparza, Sandra) Date: Wed Jun 30 10:46:58 2010 Subject: [Histonet] Histologist needed in Austin, Texas Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB041DFCB2@AUSEX2VS1.seton.org> We have a full time position open for a HT / HTL (ASCP) registered histologist, days; requires 3+ years experience and strong embedding and cutting skills. The position is at a Level 1 trauma Children's hospital that has been open for 3 years and has state of the art equipment. If you're interested in working in a team environment and would like to work in beautiful, progressive Austin, Texas please apply online at Seton.net, (Dell Children's Medical Center position) For specific questions please contact: Penny Flowers HT (ASCP) Seton Histology Technical Manager 512.324.1000 Ext. 14264 From marktarango <@t> gmail.com Wed Jun 30 11:13:40 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jun 30 11:13:45 2010 Subject: [Histonet] H. pylori RTU antibody In-Reply-To: References: Message-ID: Biocare has a rabbit polyclonal anti-HP ab and a mouse monoclonal. Which one do you use? Mark On Wed, Jun 30, 2010 at 8:05 AM, McMahon, Loralee A < Loralee_Mcmahon@urmc.rochester.edu> wrote: > Use the RTU from Biocare. It is clean and easy. You can use it with the > Dako Flex Kit. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer [ > 41dmb41@gmail.com] > Sent: Wednesday, June 30, 2010 10:41 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H. pylori RTU antibody > > I was just wondering if a lot of people use the RTU antibodies available > out > there for H. pylori. Currently, we're testing the RTU from Dako and we're > having issues with a lot of background staining. After tweaking the > protocol a lot, I've managed to get a lot of it removed, but there are > still > some issues. I was wondering if most people are making their own dilutions > to get a better stain or if the RTUs are commonplace. I appreciate the > feedback! > > Thanks, > Drew > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mabosso <@t> unipathllc.com Wed Jun 30 11:20:41 2010 From: mabosso <@t> unipathllc.com (Mary Abosso) Date: Wed Jun 30 11:22:35 2010 Subject: [Histonet] Procollagen Message-ID: <43A451981FF6634795BE83B1B5494D631BE113@exchange.unipathllc.corp> All - We are currently using Pro-COL1A1 (N17) and was wondering what others are using for a Procollagen for derm applications? Mary From annigyg <@t> gmail.com Wed Jun 30 11:24:49 2010 From: annigyg <@t> gmail.com (annigyg@gmail.com) Date: Wed Jun 30 11:24:59 2010 Subject: [Histonet] H&E stainers In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB2722@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB2722@HPEMX3.HealthPartners.int> Message-ID: <2001221518-1277915089-cardhu_decombobulator_blackberry.rim.net-1606834749-@bda242.bisx.produk.on.blackberry> Sakura wins hands down Always Empower your Business with BlackBerry? and Mobile Solutions from Etisalat -----Original Message----- From: "Webb, Dorothy L" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 30 Jun 2010 08:57:38 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] H&E stainers We are in the market for a new H&E automated stainer and am hoping I get some feedback on this request!!!!! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments "private" at Dorothy.L.Webb@Healthpartners.com Thanks much ahead of time!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Wed Jun 30 11:50:58 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Jun 30 11:51:02 2010 Subject: [Histonet] Chilling Tray for Blocks Message-ID: <57BE698966D5C54EAE8612E8941D768309080C13@EXCHANGE3.huntingtonhospital.com> I am looking for a piece of equipment that is on wheels and has a chilled top surface for chilling blocks. We have a very old one that does not work that was manufactured by Lipshaw. The top surface is completely flat and measures about 18" x 18". We ordered a Chill Tray from Mopec, but the chilling area is recessed so that you can't see the blocks when you're sitting down cutting, and it is also too small of an area. Does anyone know if there is some sort of portable chiller available out there? Laurie Colbert From cmiller <@t> physlab.com Wed Jun 30 11:51:13 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jun 30 11:51:18 2010 Subject: [Histonet] H&E slide cost In-Reply-To: <4C2B11AB.7400.0077.1@harthosp.org> References: <4C2B11AB.7400.0077.1@harthosp.org> Message-ID: There are so many variables, it all depends on your operating costs i.e. Labor, reagents, maintenance contracts and capitol equipment if applicable. I just completed my cost analysis today and it costs my department approximately $8.15 to produce 1 H&E slide, $2.55 each Special and $38.71 for each IHC Antibody. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, June 30, 2010 8:43 AM To: Histonet Subject: [Histonet] H&E slide cost I apologize for asking this question since I'm sure it's been discussed before, but I need an answer ASAP. How much does it cost to produce an H&E-stained slide from formalin-fixed tissue? We want to give this figure to our new residents and fellows who start tomorrow in order to show them how much it costs the laboratory to produce the tissue that they submit. Thanks! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From igor.deyneko <@t> gmail.com Wed Jun 30 12:01:20 2010 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Wed Jun 30 12:01:32 2010 Subject: [Histonet] Reducing blood autofluorescence in IF Message-ID: Hello everyone! I was wondering if anyone knows of an effective method of reducing/ eliminating autofluorescence of blood in IF procedures. Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA From annigyg <@t> gmail.com Wed Jun 30 12:03:14 2010 From: annigyg <@t> gmail.com (annigyg@gmail.com) Date: Wed Jun 30 12:03:27 2010 Subject: [Histonet] H&E stainers In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB272C@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB2722@HPEMX3.HealthPartners.int><2001221518-1277915089-cardhu_decombobulator_blackberry.rim.net-1606834749-@bda242.bisx.produk.on.blackberry><65365F35C0F2EF4D846EC3CA73E49C43D7EFEB272C@HPEMX3.HealthPartners.int> Message-ID: <875791713-1277917393-cardhu_decombobulator_blackberry.rim.net-578766557-@bda242.bisx.produk.on.blackberry> Easy to use Easy to program Workhorse Well supported by service engineers in my location Prefer tape coverslips - no mess and no waiting for slides to dry Prisma has optional neat half size containers and matching racks for small batches Versatile system Get sakura to demo Always best to see them operational before you decide I'm just a sakura fan Been using them for over 20years with very few disappoointments Annie Empower your Business with BlackBerry? and Mobile Solutions from Etisalat -----Original Message----- From: "Webb, Dorothy L" Date: Wed, 30 Jun 2010 11:56:11 To: 'annigyg@gmail.com' Subject: RE: [Histonet] H&E stainers And your reasons are????? I am compiling the +'s and -'s in a spreadsheet for documentation to my manager, otherwise she ends up purchasin the lowest cost item!!! Thanks for your time!! Dorothy webb -----Original Message----- From: annigyg@gmail.com [mailto:annigyg@gmail.com] Sent: Wednesday, June 30, 2010 11:25 AM To: Webb, Dorothy L; histonet-bounces@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] H&E stainers Sakura wins hands down Always Empower your Business with BlackBerry(r) and Mobile Solutions from Etisalat -----Original Message----- From: "Webb, Dorothy L" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 30 Jun 2010 08:57:38 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] H&E stainers We are in the market for a new H&E automated stainer and am hoping I get some feedback on this request!!!!! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments "private" at Dorothy.L.Webb@Healthpartners.com Thanks much ahead of time!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 41dmb41 <@t> gmail.com Wed Jun 30 12:56:44 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Wed Jun 30 12:57:09 2010 Subject: [Histonet] Re: H. pylori RTU antibody In-Reply-To: References: Message-ID: I'm getting tons of responses from people about other antibodies that work well, which I appreciate very much... However, I'm curious to hear from someone that's actually using the Dako RTU and having success... Thanks, Drew On Wed, Jun 30, 2010 at 10:41, Drew Meyer <41dmb41@gmail.com> wrote: > I was just wondering if a lot of people use the RTU antibodies available > out there for H. pylori. Currently, we're testing the RTU from Dako and > we're having issues with a lot of background staining. After tweaking the > protocol a lot, I've managed to get a lot of it removed, but there are still > some issues. I was wondering if most people are making their own dilutions > to get a better stain or if the RTUs are commonplace. I appreciate the > feedback! > > Thanks, > Drew > From wdesalvo.cac <@t> hotmail.com Wed Jun 30 13:09:43 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Jun 30 13:10:07 2010 Subject: [Histonet] H&E slide cost In-Reply-To: References: <4C2B11AB.7400.0077.1@harthosp.org>, Message-ID: I agree w/ Cheryl, there are many variables to consider and there is no "standard" cost for an H&E because we, Histotechnology, do not have a standardized process for H&E. The calculation requires precise data and will often be higher than you anticipate. I find that most labs, and the vendors, do not account for the total cost. My cost calculation is as follows: (Materials/Reagents + Instrumentation + Labor + Company Allocation) / Unit (i.e. Case/Specimen/Cassette/Slide/Stain) = Cost You will need assistance from your Finance department to acquire many of the costs associated with your instruments, labor and especially the allocation back to your department for shared services. I also feel that Cheryl's cost per slide is definitely in the ballpark. I have worked at labs, small to very large, and the cost per slide ranges in the $8.00 - $10.00 / slide, when all department expenses are accounted and the proper allocation of technical time is applied. William DeSalvo, B.S., HTL(ASCP) > From: cmiller@physlab.com > To: Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu > Date: Wed, 30 Jun 2010 11:51:13 -0500 > Subject: RE: [Histonet] H&E slide cost > CC: > > There are so many variables, it all depends on your operating costs i.e. Labor, reagents, maintenance contracts and capitol equipment if applicable. I just completed my cost analysis today and it costs my department approximately $8.15 to produce 1 H&E slide, $2.55 each Special and $38.71 for each IHC Antibody. > > Cheryl A. Miller HT(ASAP)cm > Histology/Cytology Prep Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4145 ext. 554 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun > Sent: Wednesday, June 30, 2010 8:43 AM > To: Histonet > Subject: [Histonet] H&E slide cost > > I apologize for asking this question since I'm sure it's been discussed before, but I need an answer ASAP. How much does it cost to produce an H&E-stained slide from formalin-fixed tissue? We want to give this figure to our new residents and fellows who start tomorrow in order to show them how much it costs the laboratory to produce the tissue that they submit. Thanks! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1 From jaylundgren <@t> gmail.com Wed Jun 30 15:00:46 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Jun 30 15:00:50 2010 Subject: [Histonet] Chilling Tray for Blocks In-Reply-To: <57BE698966D5C54EAE8612E8941D768309080C13@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768309080C13@EXCHANGE3.huntingtonhospital.com> Message-ID: Fill a Tupperware container with water, pop it in the freezer, and tomorrow you will have a portable block chiller, with the blocks at a perfect level for your needs, for 1/100th the price. Not trying to be a smarta$$, but.... Jay A. Lundgren, M.S., HTL (ASCP) From STapper <@t> smdc.org Wed Jun 30 15:09:37 2010 From: STapper <@t> smdc.org (Tapper, Sheila J.) Date: Wed Jun 30 15:09:53 2010 Subject: [Histonet] Tissue Processors In-Reply-To: References: <748458.69728.qm@web112603.mail.gq1.yahoo.com> <73A7ED895EE0C24D9267ED814911DF1912B75511@exchange.cmc-nh.org> <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2CC@EXCHMBC2.ad.ah.local> Message-ID: I have had a different experience with the Peloris processors. Please note Pat's warning to keep a service contract on the instrument. It is essential. We have had a single Peloris processor in our department more than 2 years. The original processor was replaced by a different unit after we had significant trouble with it. The second instrument is showing the same problems as the first. Just to be clear, we use their paraffin, their cassettes, and the usual alcohol and xylene - nothing exotic. WHEN the processor works, it does a beautiful job. However, we have had more that one occasion where the processing cycle has completed with no error codes, and the tissue is totally unprocessed. It is as if the tissue went from formalin to wax. The root cause...could not be determined. Processing problems, compounded with slow service response has left us twice with down times in excess of 5 working days. Leica has to their credit been working hard to prevent this from happening, but when you have a processor that has a reliability rate of 85%...there are issues. We were told that we would be receiving a stronger motor to prevent the plethora of rotary valve errors that we experience, but that has been delayed. We live with fluctuating volumes of solution. It appears to be a system problem. We thankfully have our Sakura VIP for backup - but the capacity is not sufficient for us to use as a replacement processor. My experience with processors was almost exclusively Sakura VIP models for the past 20 years. In that time, it was a rare occurrence for us to call for service. The transition from a reliable workhorse to a temperamental instrument has been difficult, and an experience I will not repeat. Sheila Tapper This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From darcyb <@t> slonepartners.com Wed Jun 30 15:09:59 2010 From: darcyb <@t> slonepartners.com (Darcy Bloch) Date: Wed Jun 30 15:10:15 2010 Subject: [Histonet] Histology Manager Needed! Message-ID: <3A019035-FB87-4F25-B940-C3278ABEA637@slonepartners.com> Slone Partners is seeking an histology manager for our client in South Florida. As the Histology Manager you will lead a team of technologists in a fast paced laboratory and attend to all operational needs for the department. Candidates must have appropriate certifications HT/HTL(ASCP) and 3-5 years of supervisory experience is required in addition to FL licensure. Candidates must possess effective interpersonal, communication, and leadership skills as well as excellent organizational skills. If you meet these qualifications send your resume to Darcy Bloch at darcyb@slonepartners.com or call me at 866-870-7434. Thank you, Darcy SLONEPARTNERS DARCY BLOCH - ASSOCIATE DIRECTOR OF RESEARCH Corporate Headquarters 400 Alton Road #2103 Miami Beach, Florida 33139 TOLL FREE: 866.870.7434 DIRECT: 757.271.5821 CELL: 757.761.4773 www.slonepartners.com From MSHERWOOD <@t> PARTNERS.ORG Wed Jun 30 15:35:53 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jun 30 15:36:08 2010 Subject: [Histonet] Chilling Tray for Blocks In-Reply-To: References: <57BE698966D5C54EAE8612E8941D768309080C13@EXCHANGE3.huntingtonhospital.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2443C@PHSXMB30.partners.org> We actually use a styrofoam box (used for shipping) and it stays cold longer with very little melting. We keep a couple in the -20 freezer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Wednesday, June 30, 2010 4:01 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Chilling Tray for Blocks Fill a Tupperware container with water, pop it in the freezer, and tomorrow you will have a portable block chiller, with the blocks at a perfect level for your needs, for 1/100th the price. Not trying to be a smarta$$, but.... Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From histotech <@t> imagesbyhopper.com Wed Jun 30 15:46:59 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Jun 30 15:47:36 2010 Subject: [Histonet] H&E stainers In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB2722@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB2722@HPEMX3.HealthPartners.int> Message-ID: We have the new version of the Leica XL stainer (looks just like the old one!) with the bridge-transfer station & coverslipper & love it. We had the old stainer for 10 years before getting the new one... and even managed to sell the old one! Michelle On Jun 30, 2010, at 9:57 AM, "Webb, Dorothy L" wrote: > We are in the market for a new H&E automated stainer and am hoping I > get some feedback on this request!!!!! I wold like to hear from the > techs who work with either the Prisma from Sakura or the Leica auto > or multi stainers, PLEASE!! You can email me directly if you would > like your comments "private" at Dorothy.L.Webb@Healthpartners.com > > Thanks much ahead of time!! > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and > are intended solely for the use of the individual or entity to whom > they are addressed. If you are not the intended recipient or the > individual responsible for delivering the e-mail to the intended > recipient, please be advised that you have received this e-mail in > error and that any use, dissemination, forwarding, printing, or > copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. > You will be reimbursed for reasonable costs incurred in notifying > us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Wed Jun 30 18:14:31 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jun 30 18:14:34 2010 Subject: [Histonet] Procollagen In-Reply-To: <43A451981FF6634795BE83B1B5494D631BE113@exchange.unipathllc.corp> Message-ID: Mary We use the one from Abcam ab64409, it works really nice Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Abosso Sent: Wednesday, June 30, 2010 10:21 AM To: Histonet Subject: [Histonet] Procollagen All - We are currently using Pro-COL1A1 (N17) and was wondering what others are using for a Procollagen for derm applications? Mary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Jun 30 18:36:12 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jun 30 18:36:14 2010 Subject: [Histonet] Canine bone sectioning trouble In-Reply-To: <94A0BFCE84CB0F4A84C50EAC9B02D0A9019423208F@HSC-CMS01.ad.ufl.edu> Message-ID: Overall your fixation, decalcification and processing cycle is too short. The sample size is really big, why do you need it so thick? I would first of all let the samples fix longer 24 to 48 hours or I would trim them so that they are about 3-5 mm in thickness. Either way I like fixing bone samples for 24 to 48 hours. I'm not that accustomed to using RDO decal it is hydrochloric acid based and is quicker but even bone that size would take a while to decal. We normally use a formic acid based decal and a sample size that large my take up to 2 weeks to decal. Also to process a sample that size I would be at 4 to 6 hours a station. We frequently process porcine and goat knees in 2 x 3 blocks the samples are quite thick about a cm in thickness and we will process them at 4 to 6 hours a station. My recommendation would be to decal the larger cube of bone for a period of time until you can section through it. I would then cut a 3-4 mm thick piece off the cube and process it at 1 hour per station. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schneider,Lynda S Sent: Tuesday, June 29, 2010 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Canine bone sectioning trouble Hello out there... We are having trouble sectioning canine bone. The samples are bone marrow cubes approximately 2cm thick and 1in wide. They were fixed for 15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid decalcifier. They were faced in and then surface decaled again for about 30 mins. When sectioned, much of the marrow was missing and the bone was torn and shredded. We thought that maybe the samples had not been fixed sufficiently so refixed overnight in 10% NBF again. The samples were then reprocessed as they had been originally. The processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each, paraffin x 4, 40 mins each. Again they were faced in and decaled for another 30 mins or so. This time as soon as the sections are placed on the water bath (38 degrees) they explode and/or come apart so severely sections can almost not be picked up. If sections are even obtainable they are of horrible quality. Does anyone have any suggestions? Thank you so much in advance! Lynda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histoguru <@t> yahoo.com Wed Jun 30 19:16:44 2010 From: histoguru <@t> yahoo.com (Christina Swenn) Date: Wed Jun 30 19:16:48 2010 Subject: [Histonet] unscribe Message-ID: <968945.84145.qm@web82404.mail.mud.yahoo.com> unscribe please, I do not wish to receive emails anymore, thank you