From rgrow <@t> bmnet.com Thu Jul 1 07:33:50 2010 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Thu Jul 1 07:33:59 2010 Subject: [Histonet] Position open in Maryville TN Message-ID: Blount Memorial Hospital in Maryville, TN has a full-time histology technician position open weekdays. If you are interested in working for a hospital that cares about its patients, we may be what you are looking for. We have a new (3yrs old) histology laboratory, with excellent ventilation, and ample workspace. We do approximately 12,000 cases/yr routine small and large surgical?s, & biopsies with frozens, routine H&E, special stains, and (automated) IHC antibodies. 3 tissue processors, 2 run nightly, with one for same-day turn-around cases. We are located just minutes from the beautiful Smoky Mountains National Park, Gatlinburg and Pigeon Forge. With 4 wonderful seasons, all types of outdoor activities are possible. Maryville is host to the annual Foothills Fall Festival with top name entertainment and crafts, and just 30 minutes from Knoxville's culture events and entertainment, as well as UT football, basketball, etc. General histology experience with certification preferred. Some Immunohistochemistry would be helpful. Good benefits are offered. Please visit our website at blountmemorial.org. to fill out an application and attach a resume. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. From kmerriam2003 <@t> yahoo.com Thu Jul 1 07:38:25 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Jul 1 07:38:29 2010 Subject: [Histonet] Chilling Tray for Blocks In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2443C@PHSXMB30.partners.org> References: <57BE698966D5C54EAE8612E8941D768309080C13@EXCHANGE3.huntingtonhospital.com> <073AE2BEA1C2BA4A8837AB6C4B943D9703E2443C@PHSXMB30.partners.org> Message-ID: <604558.33459.qm@web50303.mail.re2.yahoo.com> we use something like this: https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=414004-256&inE=1&highlight=414004-256 we keep several sizes in the freezer, depending on?how many blocks we need to cut.? The nice thing is that it is cheap and you can hydrate your blocks at the same time you are chilling them. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Sherwood, Margaret" To: Jay Lundgren ; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Sent: Wed, June 30, 2010 4:35:53 PM Subject: RE: [Histonet] Chilling Tray for Blocks We actually use a styrofoam box (used for shipping) and it stays cold longer with very little melting.? We keep a couple in the -20 freezer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Wednesday, June 30, 2010 4:01 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Chilling Tray for Blocks ? ? Fill a Tupperware container with water, pop it in the freezer, and tomorrow you will have a portable block chiller, with the blocks at a perfect level for your needs, for 1/100th the price. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Not trying to be a smarta$$, but.... Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Thu Jul 1 08:41:57 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu Jul 1 08:42:02 2010 Subject: [Histonet] Chilling Tray for Blocks In-Reply-To: <604558.33459.qm@web50303.mail.re2.yahoo.com> References: <57BE698966D5C54EAE8612E8941D768309080C13@EXCHANGE3.huntingtonhospital.com>, , <073AE2BEA1C2BA4A8837AB6C4B943D9703E2443C@PHSXMB30.partners.org>, <604558.33459.qm@web50303.mail.re2.yahoo.com> Message-ID: We use HistoCool trays, two sizes available, two trays for each microtome. One is always in the freezer. They work great at the microtomy bench, stay cold for long periods of time, have a styrofoam tray they sit in and have a lid. William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 1 Jul 2010 05:38:25 -0700 > From: kmerriam2003@yahoo.com > To: MSHERWOOD@PARTNERS.ORG; jaylundgren@gmail.com; laurie.colbert@huntingtonhospital.com > Subject: Re: [Histonet] Chilling Tray for Blocks > CC: histonet@lists.utsouthwestern.edu > > we use something like this: > https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=414004-256&inE=1&highlight=414004-256 > > we keep several sizes in the freezer, depending on how many blocks we need to cut. The nice thing is that it is cheap and you can hydrate your blocks at the same time you are chilling them. > > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > > > ________________________________ > From: "Sherwood, Margaret" > To: Jay Lundgren ; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Sent: Wed, June 30, 2010 4:35:53 PM > Subject: RE: [Histonet] Chilling Tray for Blocks > > We actually use a styrofoam box (used for shipping) and it stays cold longer > with very little melting. We keep a couple in the -20 freezer. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren > Sent: Wednesday, June 30, 2010 4:01 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Chilling Tray for Blocks > > > Fill a Tupperware container with water, pop it in the freezer, and > tomorrow you will have a portable block chiller, with the blocks at a > perfect level for your needs, for 1/100th the price. > > Not trying to be a > smarta$$, but.... > > > Jay A. Lundgren, M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4 From Pat.Patterson <@t> propath.com Thu Jul 1 09:04:49 2010 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Thu Jul 1 09:06:32 2010 Subject: [Histonet] Immuno Tech position Dallas Sun-Th Message-ID: <82C7248978CB50469FD6BA68EBBEFE6703AC3795@exchange.propathlab.com> IMMUNOHISTOCHEMISTRY TECHNICIAN Sun - Thurs 12am - 8:30am ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Immunohistochemistry Technician. Responsibilities include slide preparation (paraffin and frozen sections), manual IHC staining, antibody titer preparation, equipment maintenance, supply and reagent inventory maintenance, and QC/QA record maintenance. We require a minimum of 1 year full-time experience as a histotech. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Our benefits include medical, dental, company paid short-term and long-term disability insurance, a matched 401K plan and more! For consideration send resume to: ProPath, Human Resources 1355 River Bend Drive Dallas, TX 75247 Fax - 214/237-1825 Job Line - 214/237-1775 jobs@propath.com EOE Angie Viancourt Senior Human Resources Generalist ProPath(r) - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1613 214-237-1813 Fax To Learn more about ProPath, please visit http://www.ProPath.com Don't Follow the Leader. Join the Leader! From sbreeden <@t> nmda.nmsu.edu Thu Jul 1 09:12:12 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Jul 1 09:14:59 2010 Subject: [Histonet] Chilling trays Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47228@nmdamailsvr.nmda.ad.nmsu.edu> I went to eBay and found a set of three (total $12.00) stainless steel "food prep" trays, roughly 9x5x2" (LxWxH). I fill them with DI water and freeze them. I also inherited a larger stainless steel container that's 12.5x10.5x4.5" - same deal, DI water and freeze. I face blocks, put them on the ice and add a little DI water and soak for 10-15 minutes - blocks cut like a dream! They stay frozen for a l - o - n - g time! Try a local restaurant supply. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 From hfedor <@t> jhmi.edu Thu Jul 1 09:45:09 2010 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Jul 1 09:46:48 2010 Subject: [Histonet] RE: Chilling trays In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47228@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E47228@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D316BFE1BEB5@JHEMTEXVS3.win.ad.jhu.edu> You can try disecting trays. I just googled discting tray, looks like listing price is 17.00. they are the perfect size and shape for freezing. and keep blocks organized. Helen Fedor Johns Hopkins University TMA and Prostate Spore Lab Manager JHU SOM Uro/Pathology 600 N. Wolfe Street, Marburg Room 406 Baltimore MD, 21287-7065 410-614-1660 / Fax 410-614-3695 Pager 410-283-3419 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbreeden@nmda.nmsu.edu] Sent: Thursday, July 01, 2010 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chilling trays I went to eBay and found a set of three (total $12.00) stainless steel "food prep" trays, roughly 9x5x2" (LxWxH). I fill them with DI water and freeze them. I also inherited a larger stainless steel container that's 12.5x10.5x4.5" - same deal, DI water and freeze. I face blocks, put them on the ice and add a little DI water and soak for 10-15 minutes - blocks cut like a dream! They stay frozen for a l - o - n - g time! Try a local restaurant supply. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Thu Jul 1 09:50:31 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Jul 1 09:50:35 2010 Subject: [Histonet] Chilling trays In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47228@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E47228@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24445@PHSXMB30.partners.org> We used the metal trays as well for years. Switched to the styrofoam boxes and they stay frozen longer. Just a thought..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, July 01, 2010 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chilling trays I went to eBay and found a set of three (total $12.00) stainless steel "food prep" trays, roughly 9x5x2" (LxWxH). I fill them with DI water and freeze them. I also inherited a larger stainless steel container that's 12.5x10.5x4.5" - same deal, DI water and freeze. I face blocks, put them on the ice and add a little DI water and soak for 10-15 minutes - blocks cut like a dream! They stay frozen for a l - o - n - g time! Try a local restaurant supply. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From laurie <@t> conxis.com Thu Jul 1 10:22:41 2010 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Thu Jul 1 10:22:49 2010 Subject: [Histonet] MIB-1 staining Message-ID: <31BA318B1275420F859F9488CCBEC7AB.MAI@accuwebhosting.biz> Hello everyone, Happy early 4th of July. I was wondering if anyone out there is doing MIB-1 staining in mouse with human xenograft tissue? We are currently using Dako's MIB-1 clone which works well in the human ( which is what our target is) but it also has some background issues depending on the mouse line used to host the xenograft. Any thoughts? I have looked at rabbit monoclonals but I am primarily finding SP6 as the clone there. Thanks, Laurie Laurie Popp, HT ( ASCP)cm From mchadha1 <@t> gmail.com Thu Jul 1 10:46:17 2010 From: mchadha1 <@t> gmail.com (Mohit Chadha) Date: Thu Jul 1 10:46:22 2010 Subject: [Histonet] bat wing histology Message-ID: Hello everyone, This is my very first post and I am desperately looking for help. I am new to histology, so any help would be much appreciated. I am studying the peripheral sensory innervation of bat wings. As a first step, I would like to demonstrate the innervation pattern on the different parts of the wing membrane (a whole mount of the wing?). Second, I would like to demonstrate the mechanoreceptor make-up of the tiny hairs on the wing membrane. Bat wings are highly elastic, with numerous folds, a thickness of about 35-45 microns (in the species I study), a network of thin collagen bundles, and pigmented superficial epidermal layers. I could provide more information if required. Hoping to hear back from the members. Thank you. From wdesalvo.cac <@t> hotmail.com Thu Jul 1 10:49:23 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu Jul 1 10:49:41 2010 Subject: [Histonet] H&E slide cost In-Reply-To: References: <4C2B11AB.7400.0077.1@harthosp.org>, , , Message-ID: Thank you for the direction to CAP, but I am registered on the CAP site and I have not been able to find a cost per slide calculation for H&E. I would welcome the assistance of CAP to help standardize the process and provide specific direction and guidance. On the CAP site there is a paper by Dr. Hendricks, Cleveland Clinic. He does discuss cost per slide associated w/ digital pathology and capturing the FTE time required to scan, but even that discussion does not consider the "total" cost for a slide. The difficulty is that there is no standardization of process or procedure and the digital age means an increase in labor costs for the technical end of the process. We are rapidly moving to digital pathology and our lack of standardization will create many problems similar to those that are experienced when labs perform IHC. How do we progress Histotechnology from Open Standards to Interoperability, a must if we truly want to compare results? What continues to concern me most, many Histology labs not only do not have an accurate cost per slide for H&E, they do not have a cost for special staining (Histochemical techniques) or IHC and they cannot break down each step of their process to consider the cost. Too many Histology labs rely on their vendors to provide costing information. In the past, there have always been enough dollars available in the reimbursements to more than cover the "technical component", but as we move forward and to more precise and complex procedures, while requiring better trained and educated technologists, the dollars will quickly become inadequate. We cannot continue to function as we do today. In the future state of Histology and Histotechnology, it will be essential that each Histology lab be able to efficiently manage the technical process, remove waste and maintain a healthy profit margin, while providing excellent quality and patient care. There must be an increase in automation of instruments and even though we do not perform our tests in a test tube, more tests performed in Histology will be at the IVD level. We must become better at Histotechnology to continue to perform more complex tests and we will also need a better partnering of the Histotechnologist and Pathologist to keep up with the rapid advancement of technology. Unfortunately, we will have many Histology labs struggle with this change and many will go out of business or be consolidated into larger labs that can afford the technology, through efficient process improvement, and control of quality and costs. The bright side is this is an exciting time for Histology and Histotechnology and I am certainly excited about the future. William DeSalvo, B.S., HTL(ASCP) Subject: RE: [Histonet] H&E slide cost Date: Wed, 30 Jun 2010 14:26:31 -0700 From: JHowery2@yrmc.org To: wdesalvo.cac@hotmail.com Register on the CAP site. They have many documents on calculation of different costs in the laboratory. From: histonet-bounces@lists.utsouthwestern.edu on behalf of WILLIAM DESALVO Sent: Wed 6/30/2010 11:09 AM To: cmiller@physlab.com; rcartun@harthosp.org; histonet Subject: RE: [Histonet] H&E slide cost I agree w/ Cheryl, there are many variables to consider and there is no "standard" cost for an H&E because we, Histotechnology, do not have a standardized process for H&E. The calculation requires precise data and will often be higher than you anticipate. I find that most labs, and the vendors, do not account for the total cost. My cost calculation is as follows: (Materials/Reagents + Instrumentation + Labor + Company Allocation) / Unit (i.e. Case/Specimen/Cassette/Slide/Stain) = Cost You will need assistance from your Finance department to acquire many of the costs associated with your instruments, labor and especially the allocation back to your department for shared services. I also feel that Cheryl's cost per slide is definitely in the ballpark. I have worked at labs, small to very large, and the cost per slide ranges in the $8.00 - $10.00 / slide, when all department expenses are accounted and the proper allocation of technical time is applied. William DeSalvo, B.S., HTL(ASCP) > From: cmiller@physlab.com > To: Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu > Date: Wed, 30 Jun 2010 11:51:13 -0500 > Subject: RE: [Histonet] H&E slide cost > CC: > > There are so many variables, it all depends on your operating costs i.e. Labor, reagents, maintenance contracts and capitol equipment if applicable. I just completed my cost analysis today and it costs my department approximately $8.15 to produce 1 H&E slide, $2.55 each Special and $38.71 for each IHC Antibody. > > Cheryl A. Miller HT(ASAP)cm > Histology/Cytology Prep Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4145 ext. 554 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun > Sent: Wednesday, June 30, 2010 8:43 AM > To: Histonet > Subject: [Histonet] H&E slide cost > > I apologize for asking this question since I'm sure it's been discussed before, but I need an answer ASAP. How much does it cost to produce an H&E-stained slide from formalin-fixed tissue? We want to give this figure to our new residents and fellows who start tomorrow in order to show them how much it costs the laboratory to produce the tissue that they submit. Thanks! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1 From gentras <@t> auburn.edu Thu Jul 1 11:24:20 2010 From: gentras <@t> auburn.edu (Atoska Gentry) Date: Thu Jul 1 11:24:48 2010 Subject: [Histonet] Re: replacement Turtox Internal Ear, cochlea slides References: <4C2B67DC.5876.00D9.0@auburn.edu> Message-ID: <4C2C7AE4.C676.0026.0@auburn.edu> Hello! please see below inquiry from a colleague. Thanks! Atoska Our histology class is looking for replacements for old Turtox (Guinea Pig) Internal Ear, cochlea slides and was wondering if anyone knew a company who supplied these slides or tissue blocks? I don't think it has to be guinea pig but I haven't been able to locate internal ear slides from any species. Any help would be appreciated. Thanks! ~~~~~~~~~~~~~~~ Michelle (Shelly) Aono Histology Technician IV CVM & AU Staff Advisory Council Representative Auburn University CVM-APP 212 Greene Hall, Auburn, AL 36832 (334) 844-5594 From LINDA.MARGRAF <@t> childrens.com Thu Jul 1 12:26:45 2010 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Thu Jul 1 12:27:06 2010 Subject: [Histonet] Dallas job Message-ID: <4C2C8985.F783.00DA.0@childrens.com> Here's a job someone asked me to post on Histonet (Please don't reply to me ....Thanks LM) OPEN POSITION FOR AN EXPERIENCED HISTOTECHNOLOGIST IN THE GREATER DALLAS, TX AREA: Customer Liaison/Technical Manager 70K+Bonus My name is Lisa Chubinsky and I am an Executive Recruiter with Scientific Professional Search. My client, which is a Medical Products company that manufactures Histotechnology products, currently seeks a Customer Liaison/Technical Manager to oversee Technical Support, Customer Outreach, Sales Support and Marketing and Product Development. The Technical Manager will be expected to advance the image and reputation of the Company through frequent speaking engagements at national and regional trade shows. The Technical Manager will be given ownership of the Technical Manual in order to make sure its contents are accurate, up to date, and being used effectively as a marketing and education piece. Other customer outreach projects might also include a periodic newsletter and development and nurturing of a Histotech Advisory Board to be used by the Company to stay current with issues and challenges faced by Histotechs. For immediate consideration, please contact Lisa Chubinsky, CPC, Scientific Professional Search, 203-834-2100x12, Lisa@spexecsearch.com Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From histonet.nospam <@t> vneubert.com Thu Jul 1 12:39:45 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Thu Jul 1 12:39:58 2010 Subject: [Histonet] bat wing histology In-Reply-To: References: Message-ID: <4C2CD2E1.70207@vneubert.com> Make that an open discussion on the list, no "under cover posting" if possible, please! :) > Hello everyone, > > This is my very first post and I am desperately looking for help. I am new > to histology, so any help would be much appreciated. > > I am studying the peripheral sensory innervation of bat wings. As a first > step, I would like to demonstrate the innervation pattern on the different > parts of the wing membrane (a whole mount of the wing?). Second, I would > like to demonstrate the mechanoreceptor make-up of the tiny hairs on the > wing membrane. > > Bat wings are highly elastic, with numerous folds, a thickness of about > 35-45 microns (in the species I study), a network of thin collagen bundles, > and pigmented superficial epidermal layers. > I could provide more information if required. > > Hoping to hear back from the members. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From beth.villarreal <@t> novartis.com Thu Jul 1 12:50:38 2010 From: beth.villarreal <@t> novartis.com (beth.villarreal@novartis.com) Date: Thu Jul 1 12:50:58 2010 Subject: [Histonet] control tissue for Hall's bile stain In-Reply-To: <201007011740.o61HerRd027776@ch1ssmenov01.novartis.com> Message-ID: Does anyone know a commercial source for control tissue for bile/bilirubin? Species does not matter. Thanks! Beth Villarreal, HT(ASCP) Novartis Institutes for BioMedical PCS, Discovery Pathology, US USCA, 611-7104B Novartis Institutes for BioMedical Research, Inc. 100 Technology Square Cambridge, MA 02139 USA Phone: +1 617 8714725 Email : beth.villarreal@novartis.com From Reuel.Cornelia <@t> tsrh.org Thu Jul 1 13:30:45 2010 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu Jul 1 13:31:14 2010 Subject: [Histonet] MMP9 and MMP13 on pig tissue Message-ID: <4C2C9885020000C50007C7B7@mail.TSRH.ORG> Does anybody out there working on MMP9 and 13 on Pig tissue. Where do you purchase your antibody. Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From mary.gessford <@t> spcorp.com Thu Jul 1 13:37:29 2010 From: mary.gessford <@t> spcorp.com (Gessford, Mary) Date: Thu Jul 1 13:37:37 2010 Subject: [Histonet] control tissue for Hall's bile stain In-Reply-To: References: <201007011740.o61HerRd027776@ch1ssmenov01.novartis.com> Message-ID: Newcomer Supply has bile/liver control slides newcomersupply.com cat.#4060A set of ten, 4060B set of 98 Mary Gessford Senior Scientist Supervisor - Anatomic Pathology Toxicology and Pathology Scientific Operations T: 908-473-4358 MERCK, Summit, NJ mary.gessford@spcorp.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of beth.villarreal@novartis.com Sent: Thursday, July 01, 2010 01:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] control tissue for Hall's bile stain Does anyone know a commercial source for control tissue for bile/bilirubin? Species does not matter. Thanks! Beth Villarreal, HT(ASCP) Novartis Institutes for BioMedical PCS, Discovery Pathology, US USCA, 611-7104B Novartis Institutes for BioMedical Research, Inc. 100 Technology Square Cambridge, MA 02139 USA Phone: +1 617 8714725 Email : beth.villarreal@novartis.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From mweirauch <@t> crittenton.com Thu Jul 1 14:23:22 2010 From: mweirauch <@t> crittenton.com (Maray Weirauch) Date: Thu Jul 1 14:24:01 2010 Subject: [Histonet] Charging question Message-ID: A long time ago I attended a pathology charging workshop with Dennis Paget. He had stated that even though tubes and ovaries are a 'go-with' to the uterus, if they were submitted for frozen section for separate evaluation they could be charged. We have a case now that included uterus with metastatic carcinoma, both ovaries with serous carcinoma, both fallopian tubes with metastatic serous carcinoma and pelvic nodes free of involvement. Our pathologist believes we should charge 88309 (uterus), 88307 x2 (ovaries), 88305 x2 (fallopian tubes), 88307 (lymph node resection), and the frozen section charges. I'm not sure the tubes can support a separate charge. Any comments on this? Thanks in advance! Maray From Allison_Scott <@t> hchd.tmc.edu Thu Jul 1 14:36:45 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Jul 1 14:36:54 2010 Subject: [Histonet] Cap Question: 2 identifiers for slides Message-ID: <1872B4A455B7974391609AD8034C79FC026DFC8B@LBEXCH01.hchd.local> Is is a CAP requirement to have 2 identifiers on surgical slides? Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From flnails <@t> texaschildrens.org Thu Jul 1 15:09:42 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Jul 1 15:09:53 2010 Subject: [Histonet] RE: Cap Question: 2 identifiers for slides In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFC8B@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFC8B@LBEXCH01.hchd.local> Message-ID: Not yet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, July 01, 2010 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cap Question: 2 identifiers for slides Is is a CAP requirement to have 2 identifiers on surgical slides? Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From JWeems <@t> sjha.org Thu Jul 1 15:31:36 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jul 1 15:31:43 2010 Subject: [Histonet] RE: Cap Question: 2 identifiers for slides In-Reply-To: References: <1872B4A455B7974391609AD8034C79FC026DFC8B@LBEXCH01.hchd.local> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015E968001@CHEXCMS10.one.ads.che.org> Joint Commission does... Use case number and name. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Thursday, July 01, 2010 16:10 To: 'Scott, Allison D'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cap Question: 2 identifiers for slides Not yet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, July 01, 2010 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cap Question: 2 identifiers for slides Is is a CAP requirement to have 2 identifiers on surgical slides? Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From k84as <@t> yahoo.com Fri Jul 2 04:23:27 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Jul 2 04:23:31 2010 Subject: [Histonet] massons question Message-ID: <176197.69851.qm@web112615.mail.gq1.yahoo.com> hi all i'm going to stain some slides of small intestin which were fixed in susa. i'm going to stain it by Massons trichrome. i have read that slides should be secondry fixed in Bouin's or saturated picric solution. ? does it mean fixation of processed tissue after cutting with microtom?? how long?? could you please share me your protocol for massons ? thanks in advance ? mohamed histology dep. faculty of vet. med. cairo univ.- Egypt From BSullivan <@t> shorememorial.org Fri Jul 2 05:36:49 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Fri Jul 2 05:39:10 2010 Subject: [Histonet] massons question In-Reply-To: <176197.69851.qm@web112615.mail.gq1.yahoo.com> Message-ID: Bouin's is used as a mordant in this stain. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 mohamed abd el razik To Sent by: Histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] massons question 07/02/2010 05:23 AM hi all i'm going to stain some slides of small intestin which were fixed in susa. i'm going to stain it by Massons trichrome. i have read that slides should be secondry fixed in Bouin's or saturated picric solution. does it mean fixation of processed tissue after cutting with microtom?? how long?? could you please share me your protocol for massons thanks in advance mohamed histology dep. faculty of vet. med. cairo univ.- Egypt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Fri Jul 2 10:23:08 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Jul 2 10:23:16 2010 Subject: [Histonet] ER and PR validation Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43D7EFEB2744@HPEMX3.HealthPartners.int> In a recent article written by 4 pathologists, including Dr. Elizabeth Hammon and Dr. Patrick Fitzgibbons, the recommendations are now including testing with a lab that has validated it's assay against clinical outcomes. How is everyone planning on fulfilling this requirement inasmuch as we are not aware of any lab in our metro area with this kind of compliance. Thanks, as always, for any guidance in this area! If you would like a reprint of this article, email is pfitz@stjoe.org and the article is entitle "Recommendations for Validating Estrogen and Progesterone Receptor immunohistochemistry Assays". Dorothy Webb, HT Regions Histology Technical Supervisor ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From llewllew <@t> shaw.ca Fri Jul 2 10:46:33 2010 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Jul 2 10:46:39 2010 Subject: [Histonet] massons question In-Reply-To: <176197.69851.qm@web112615.mail.gq1.yahoo.com> References: <176197.69851.qm@web112615.mail.gq1.yahoo.com> Message-ID: <163D11C6632C4C14967EE222A2888127@BryanPC> SuSa has a high mercuric chloride content, and tissues fixed in high mercury fixatives do not require secondary fixation in picric acid (Bouin). The mercuric chloride fixes in a manner which improves staining with acid dyes. For Masson's trichrome on tissues fixed with simple formalin variants, secondary fixation in Bouin or picric acid at 60C for an hour is recommended after baking on and dewaxing. Bryan Llewellyn ----- Original Message ----- From: "mohamed abd el razik" To: Sent: Friday, July 02, 2010 2:23 AM Subject: [Histonet] massons question hi all i'm going to stain some slides of small intestin which were fixed in susa. i'm going to stain it by Massons trichrome. i have read that slides should be secondry fixed in Bouin's or saturated picric solution. does it mean fixation of processed tissue after cutting with microtom?? how long?? could you please share me your protocol for massons thanks in advance mohamed histology dep. faculty of vet. med. cairo univ.- Egypt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Fri Jul 2 10:59:01 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Fri Jul 2 10:59:47 2010 Subject: [Histonet] BILLING QUESTION Message-ID: Histonetters: If you do some work for another hospital (Histology) can you bill the hospital or do you have to bill the patient directly? Is there a statute or Regulation out there about this? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From rsrichmond <@t> gmail.com Fri Jul 2 12:48:52 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Jul 2 12:48:58 2010 Subject: [Histonet] Re: control tissue for Hall's bile stain Message-ID: Hall's bile stain - not really a stain but a reaction, like the Perls prussian blue reaction for iron - uses trichloracetic acid and ferric chloride to oxidize yellow-brown bilirubin to bright green biliverdin. Or so I've been told - I've never seen one. Something I've wondered about for a long time: could Hall's reaction be used to demonstrate meconium in macrophages in the chorion of the human placenta? These can be hard to see, particularly if you don't have a very good microscope. - If this would work, it would provide a ready source for controls, at least for the hospital pathologist. Bob Richmond Samurai Pathologist Knoxville TN From cpyse <@t> x-celllab.com Fri Jul 2 12:51:51 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Jul 2 12:52:01 2010 Subject: [Histonet] BILLING QUESTION In-Reply-To: References: Message-ID: <000001cb1a0f$40ca1c10$c25e5430$@com> We have a contract with the hospitals, this comes from our billing manger. We bill the hospitals, then they bill the patient. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Friday, July 02, 2010 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BILLING QUESTION Histonetters: If you do some work for another hospital (Histology) can you bill the hospital or do you have to bill the patient directly? Is there a statute or Regulation out there about this? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMyers1 <@t> aol.com Fri Jul 2 12:54:26 2010 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Fri Jul 2 12:55:08 2010 Subject: [Histonet] ER and PR validation Message-ID: <8f84.3cbd32f0.395f81d2@aol.com> This paper can also be downloaded, indirectly, from the CAP's website: _http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_ (http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) Happy (re-)validating, Joe ------------------------------ Message: 12 Date: Fri, 2 Jul 2010 10:23:08 -0500 From: "Webb, Dorothy L" Subject: [Histonet] ER and PR validation To: _'histonet@lists.utsouthwestern.edu'_ (mailto:'histonet@lists.utsouthwestern.edu') In a recent article written by 4 pathologists, including Dr. Elizabeth Hammond and Dr. Patrick Fitzgibbons, the recommendations are now including testing with a lab that has validated it's assay against clinical outcomes. How is everyone planning on fulfilling this requirement inasmuch as we are not aware of any lab in our metro area with this kind of compliance. Thanks, as always, for any guidance in this area! If you would like a reprint of this article, email is pfitz@stjoe.org and the article is entitle "Recommendations for Validating Estrogen and Progesterone Receptor immunohistochemistry Assays". Dorothy Webb, HT Regions Histology Technical Supervisor From thomas.crowell <@t> novartis.com Fri Jul 2 14:32:28 2010 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Fri Jul 2 14:32:44 2010 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 07/02/2010 and will not return until 07/13/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From PAGE.BALUCH <@t> asu.edu Fri Jul 2 15:14:00 2010 From: PAGE.BALUCH <@t> asu.edu (Debra Baluch) Date: Fri Jul 2 15:14:05 2010 Subject: [Histonet] Looking for MIN6 cell line In-Reply-To: <8f84.3cbd32f0.395f81d2@aol.com> References: <8f84.3cbd32f0.395f81d2@aol.com> Message-ID: <80BBC916BEC29A498784913D007DD4A24DBDC0A1FD@EX10.asurite.ad.asu.edu> Does anyone know where I can obtain the MIN6 beta cell line derived from mouse pancreas? They are listed and used in many papers but do not list the source. I have tried ATCC and suggested cell banks in ECACC (England), DSMZ (Germany), and JCRB (Japan) but they are not listed. Thanks, Page Arizona State University/SoLS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMyers1@aol.com Sent: Friday, July 02, 2010 10:54 AM To: histonet@lists.utsouthwestern.edu Cc: Dorothy.L.Webb@HealthPartners.Com Subject: RE: [Histonet] ER and PR validation This paper can also be downloaded, indirectly, from the CAP's website: _http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_ (http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) Happy (re-)validating, Joe ------------------------------ Message: 12 Date: Fri, 2 Jul 2010 10:23:08 -0500 From: "Webb, Dorothy L" Subject: [Histonet] ER and PR validation To: _'histonet@lists.utsouthwestern.edu'_ (mailto:'histonet@lists.utsouthwestern.edu') In a recent article written by 4 pathologists, including Dr. Elizabeth Hammond and Dr. Patrick Fitzgibbons, the recommendations are now including testing with a lab that has validated it's assay against clinical outcomes. How is everyone planning on fulfilling this requirement inasmuch as we are not aware of any lab in our metro area with this kind of compliance. Thanks, as always, for any guidance in this area! If you would like a reprint of this article, email is pfitz@stjoe.org and the article is entitle "Recommendations for Validating Estrogen and Progesterone Receptor immunohistochemistry Assays". Dorothy Webb, HT Regions Histology Technical Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Fri Jul 2 15:14:36 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Jul 2 15:14:42 2010 Subject: [Histonet] bat wing histology Message-ID: Hi, I do hope you are looking at cross sections of the wing and not the flat. That would be very difficult indeed. For good cross sections I would try a "Swiss Roll". This is a way of demonstrating a large amount of cross sectional area in small space. Take the membrane and fix it by submersion in the fixative of your choice. Prior to processing roll the whole membrane up then cut the membrane log into sections small enough to fit in a cassette. You can use foam biopsy pads to support this shape. Embed it and section it on edge to show a long coiled membrane. The hairs should be able to be displayed in this way as well. To show a lot of membrane at the same time you could place multiple rolls in one cassette. This should work well. Good Luck, Amos Message: 21 Date: Thu, 1 Jul 2010 11:46:17 -0400 From: Mohit Chadha Subject: [Histonet] bat wing histology To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello everyone, This is my very first post and I am desperately looking for help. I am new to histology, so any help would be much appreciated. I am studying the peripheral sensory innervation of bat wings. As a first step, I would like to demonstrate the innervation pattern on the different parts of the wing membrane (a whole mount of the wing?). Second, I would like to demonstrate the mechanoreceptor make-up of the tiny hairs on the wing membrane. Bat wings are highly elastic, with numerous folds, a thickness of about 35-45 microns (in the species I study), a network of thin collagen bundles, and pigmented superficial epidermal layers. I could provide more information if required. Hoping to hear back from the members. Thank you. From sfeher <@t> CMC-NH.ORG Fri Jul 2 16:18:14 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Jul 2 16:18:20 2010 Subject: [Histonet] BILLING QUESTION In-Reply-To: <000001cb1a0f$40ca1c10$c25e5430$@com> References: <000001cb1a0f$40ca1c10$c25e5430$@com> Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B75578@exchange.cmc-nh.org> You can contract for services to another lab or hospital and depending upon the terms of the contract, you may be able to bill the patient. For example, you send all of your HER2's and ER/PR to another lab to be stained and quantified into a score. You may contract with the other lab for them to charge you a flat fee for the service and you will bill the patient for 88361x3. The other side is that you can contract for the other hospital to do the test, bill the patient and your hospital pays no fee but misses out on some of the compensation. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, July 02, 2010 1:52 PM To: 'Sara Baldwin/mhhcc.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BILLING QUESTION We have a contract with the hospitals, this comes from our billing manger. We bill the hospitals, then they bill the patient. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Friday, July 02, 2010 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BILLING QUESTION Histonetters: If you do some work for another hospital (Histology) can you bill the hospital or do you have to bill the patient directly? Is there a statute or Regulation out there about this? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vavalos <@t> allergydermatology.com Fri Jul 2 17:20:52 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Fri Jul 2 17:21:07 2010 Subject: [Histonet] xylene substitute Message-ID: <000301cb1a34$dbdd5c10$93981430$@com> I am taking a chance that this is still a working email!! I came across an old post on histonet regarding Xylene subs with a linear stainer. I was wondering if you made the switch, and if so could you share the brand and your H&E protocol. I have had some issues with Eosin bleeding, slides very eosinophilic and blue haze on the slide. Not all at once but its enough to drive me crazy!! Your help would be appreciated. V.Avalos ADS, INC Fax:602-277-2134 From pruegg <@t> ihctech.net Sun Jul 4 11:42:47 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Jul 4 11:43:40 2010 Subject: SPAM-LOW: [Histonet] Re: replacement Turtox Internal Ear, cochlea slides In-Reply-To: <4C2C7AE4.C676.0026.0@auburn.edu> References: <4C2B67DC.5876.00D9.0@auburn.edu> <4C2C7AE4.C676.0026.0@auburn.edu> Message-ID: <7568A26877EB4C92BD7F83FD0C984AE6@prueggihctechlt> Wow that is going to be a tuff one, back in the day I tried to cut Petris pyramid from the inter ear and was told it was the densest/hardest bone in the body and it sure seemed to be. Even decalcified we had to embed it in plastic and use permanent tungsten carbide D profile knives to cut any sections. I am not at the university anymore so can't access those slides or I would try to help you out. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska Gentry Sent: Thursday, July 01, 2010 10:24 AM To: histonet@pathology.swmed.edu Subject: SPAM-LOW: [Histonet] Re: replacement Turtox Internal Ear, cochlea slides Hello! please see below inquiry from a colleague. Thanks! Atoska Our histology class is looking for replacements for old Turtox (Guinea Pig) Internal Ear, cochlea slides and was wondering if anyone knew a company who supplied these slides or tissue blocks? I don't think it has to be guinea pig but I haven't been able to locate internal ear slides from any species. Any help would be appreciated. Thanks! ~~~~~~~~~~~~~~~ Michelle (Shelly) Aono Histology Technician IV CVM & AU Staff Advisory Council Representative Auburn University CVM-APP 212 Greene Hall, Auburn, AL 36832 (334) 844-5594 From pruegg <@t> ihctech.net Sun Jul 4 11:46:25 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Jul 4 11:47:15 2010 Subject: SPAM-LOW: Re: [Histonet] Chilling Tray for Blocks In-Reply-To: <604558.33459.qm@web50303.mail.re2.yahoo.com> References: <57BE698966D5C54EAE8612E8941D768309080C13@EXCHANGE3.huntingtonhospital.com><073AE2BEA1C2BA4A8837AB6C4B943D9703E2443C@PHSXMB30.partners.org> <604558.33459.qm@web50303.mail.re2.yahoo.com> Message-ID: <8178EEB0DB06440AB8A5250A81E75CE5@prueggihctechlt> Yep that is what I do, I use the plastic containers I get salad or other stuff in I would throw away, fill it and freeze it, I add some water to it and put the blocks on the frozen tray and they get cold and hydrated at the same time. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Thursday, July 01, 2010 6:38 AM To: Sherwood, Margaret; Jay Lundgren; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Chilling Tray for Blocks we use something like this: https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=414004-256&in E=1&highlight=414004-256 we keep several sizes in the freezer, depending on?how many blocks we need to cut.? The nice thing is that it is cheap and you can hydrate your blocks at the same time you are chilling them. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Sherwood, Margaret" To: Jay Lundgren ; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Sent: Wed, June 30, 2010 4:35:53 PM Subject: RE: [Histonet] Chilling Tray for Blocks We actually use a styrofoam box (used for shipping) and it stays cold longer with very little melting.? We keep a couple in the -20 freezer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Wednesday, June 30, 2010 4:01 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Chilling Tray for Blocks ? ? Fill a Tupperware container with water, pop it in the freezer, and tomorrow you will have a portable block chiller, with the blocks at a perfect level for your needs, for 1/100th the price. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Not trying to be a smarta$$, but.... Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Jul 4 11:50:17 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Jul 4 11:51:05 2010 Subject: SPAM-LOW: RE: [Histonet] Canine bone sectioning trouble In-Reply-To: References: <94A0BFCE84CB0F4A84C50EAC9B02D0A9019423208F@HSC-CMS01.ad.ufl.edu> Message-ID: <068F5447C51843FA9080A848C29DF7D3@prueggihctechlt> Oh my, I agree with Liz, your samples are way too thick and your process is way too short. Even for 3-4mm thickness bone I fix for 24-72 hours and then decal in 5% formic acid usually overnight on a platform shaker. I assume RDO decals a lot faster but not that fast, I do not use RDO because I think the formic acid which may be slower better preserves the antigens for IHC. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, June 30, 2010 5:36 PM To: Schneider,Lynda S; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] Canine bone sectioning trouble Overall your fixation, decalcification and processing cycle is too short. The sample size is really big, why do you need it so thick? I would first of all let the samples fix longer 24 to 48 hours or I would trim them so that they are about 3-5 mm in thickness. Either way I like fixing bone samples for 24 to 48 hours. I'm not that accustomed to using RDO decal it is hydrochloric acid based and is quicker but even bone that size would take a while to decal. We normally use a formic acid based decal and a sample size that large my take up to 2 weeks to decal. Also to process a sample that size I would be at 4 to 6 hours a station. We frequently process porcine and goat knees in 2 x 3 blocks the samples are quite thick about a cm in thickness and we will process them at 4 to 6 hours a station. My recommendation would be to decal the larger cube of bone for a period of time until you can section through it. I would then cut a 3-4 mm thick piece off the cube and process it at 1 hour per station. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schneider,Lynda S Sent: Tuesday, June 29, 2010 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Canine bone sectioning trouble Hello out there... We are having trouble sectioning canine bone. The samples are bone marrow cubes approximately 2cm thick and 1in wide. They were fixed for 15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid decalcifier. They were faced in and then surface decaled again for about 30 mins. When sectioned, much of the marrow was missing and the bone was torn and shredded. We thought that maybe the samples had not been fixed sufficiently so refixed overnight in 10% NBF again. The samples were then reprocessed as they had been originally. The processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each, paraffin x 4, 40 mins each. Again they were faced in and decaled for another 30 mins or so. This time as soon as the sections are placed on the water bath (38 degrees) they explode and/or come apart so severely sections can almost not be picked up. If sections are even obtainable they are of horrible quality. Does anyone have any suggestions? Thank you so much in advance! Lynda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Jul 4 11:57:39 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Jul 4 11:58:36 2010 Subject: SPAM-LOW: Re: [Histonet] Unstained Slides In-Reply-To: <4C289B95.7400.0077.1@harthosp.org> References: <4C289B95.7400.0077.1@harthosp.org> Message-ID: I do not bake my unstained slides for IHC controls and store them at 4dc. I bake them just before use. They do last longer this way but Richard is right, there is no telling because it all depends on the fixation, target protein stability and storage conditions. It is always a good idea to cut fresh sections if the stored unstained sections do not perform as expected. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, June 28, 2010 10:55 AM To: histonet@lists.utsouthwestern.edu; Rick.Garnhart@memorialhealthsystem.com Subject: SPAM-LOW: Re: [Histonet] Unstained Slides We bake our unstains at 60 degrees C. for 30 minutes prior to filing at RT. There is no set rule for stability. It all depends on fixation, the nature of the protein target, and storage conditions. I've seen absolutely spectacular immunoreactivity on unstained slides stored at RT for 20 years (and longer) and I've seen reduced immunoreactivity in unstained slides stored for as little as 4 weeks. We will always attempt to stain unstained slides when available; however, if the lesion or tumor is negative, and there is no internal control, you better cut fresh sections. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 6/28/2010 11:54 AM >>> Histoland, How is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfitz <@t> 007group.com Mon Jul 5 22:33:55 2010 From: cfitz <@t> 007group.com (Cathy) Date: Mon Jul 5 22:33:58 2010 Subject: [Histonet] Ventana Ultra Message-ID: <9E39F6A091E54A378D31EC7E60494882@your8ba846406f> We are in the process of upgrading our immuno stainers to the Ventana Ultra. I am interested in how people validate a new instrument and how the Ultra was integrated or changed lab workflow. Cathy Kelowna, B.C. From aaperghis <@t> uspath.com Tue Jul 6 07:21:51 2010 From: aaperghis <@t> uspath.com (Adrienne Aperghis Kavanagh) Date: Tue Jul 6 07:22:02 2010 Subject: [Histonet] Re: Turtox Internal Ear, cochlea slides In-Reply-To: <9649152.172813.1278418676104.JavaMail.root@mail3d.brinkster.com> Message-ID: <5462721.172834.1278418911804.JavaMail.root@mail3d.brinkster.com> Hi Michelle, I worked for a company called WARD'S Natural Science in Rochester, NY; they produce/provide science education materials. I can't say I ever produced the internal ear slide myself, but I can recall others in the department working on something similar. I would give them a call at 1-800-962-2660 and ask to speak with a tech or supervisor in the microscope slides department. Even if they don't normally carry what you're looking for, I am sure they would be happy to try and provide you with what you want. I hope you find what you're looking for! Best of luck. Adrienne Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Sunday, July 4, 2010 1:45:10 PM Subject: Histonet Digest, Vol 80, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: SPAM-LOW: [Histonet] Re: replacement Turtox Internal Ear, cochlea slides (Patsy Ruegg) 2. RE: SPAM-LOW: Re: [Histonet] Chilling Tray for Blocks (Patsy Ruegg) 3. RE: SPAM-LOW: RE: [Histonet] Canine bone sectioning trouble (Patsy Ruegg) 4. RE: SPAM-LOW: Re: [Histonet] Unstained Slides (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Sun, 4 Jul 2010 10:42:47 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] Re: replacement Turtox Internal Ear, cochlea slides To: "'Atoska Gentry'" , Message-ID: <7568A26877EB4C92BD7F83FD0C984AE6@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" Wow that is going to be a tuff one, back in the day I tried to cut Petris pyramid from the inter ear and was told it was the densest/hardest bone in the body and it sure seemed to be. Even decalcified we had to embed it in plastic and use permanent tungsten carbide D profile knives to cut any sections. I am not at the university anymore so can't access those slides or I would try to help you out. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska Gentry Sent: Thursday, July 01, 2010 10:24 AM To: histonet@pathology.swmed.edu Subject: SPAM-LOW: [Histonet] Re: replacement Turtox Internal Ear, cochlea slides Hello! please see below inquiry from a colleague. Thanks! Atoska Our histology class is looking for replacements for old Turtox (Guinea Pig) Internal Ear, cochlea slides and was wondering if anyone knew a company who supplied these slides or tissue blocks? I don't think it has to be guinea pig but I haven't been able to locate internal ear slides from any species. Any help would be appreciated. Thanks! ~~~~~~~~~~~~~~~ Michelle (Shelly) Aono Histology Technician IV CVM & AU Staff Advisory Council Representative Auburn University CVM-APP 212 Greene Hall, Auburn, AL 36832 (334) 844-5594 ------------------------------ Message: 2 Date: Sun, 4 Jul 2010 10:46:25 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: Re: [Histonet] Chilling Tray for Blocks To: "'Kim Merriam'" , "'Sherwood, Margaret'" , "'Jay Lundgren'" , "'Laurie Colbert'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8178EEB0DB06440AB8A5250A81E75CE5@prueggihctechlt> Content-Type: text/plain; charset="iso-8859-1" Yep that is what I do, I use the plastic containers I get salad or other stuff in I would throw away, fill it and freeze it, I add some water to it and put the blocks on the frozen tray and they get cold and hydrated at the same time. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Thursday, July 01, 2010 6:38 AM To: Sherwood, Margaret; Jay Lundgren; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Chilling Tray for Blocks we use something like this: https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=414004-256&in E=1&highlight=414004-256 we keep several sizes in the freezer, depending on?how many blocks we need to cut.? The nice thing is that it is cheap and you can hydrate your blocks at the same time you are chilling them. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Sherwood, Margaret" To: Jay Lundgren ; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Sent: Wed, June 30, 2010 4:35:53 PM Subject: RE: [Histonet] Chilling Tray for Blocks We actually use a styrofoam box (used for shipping) and it stays cold longer with very little melting.? We keep a couple in the -20 freezer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Wednesday, June 30, 2010 4:01 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Chilling Tray for Blocks ? ? Fill a Tupperware container with water, pop it in the freezer, and tomorrow you will have a portable block chiller, with the blocks at a perfect level for your needs, for 1/100th the price. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Not trying to be a smarta$$, but.... Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sun, 4 Jul 2010 10:50:17 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: RE: [Histonet] Canine bone sectioning trouble To: "'Liz Chlipala'" , "'Schneider,Lynda S'" , Message-ID: <068F5447C51843FA9080A848C29DF7D3@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" Oh my, I agree with Liz, your samples are way too thick and your process is way too short. Even for 3-4mm thickness bone I fix for 24-72 hours and then decal in 5% formic acid usually overnight on a platform shaker. I assume RDO decals a lot faster but not that fast, I do not use RDO because I think the formic acid which may be slower better preserves the antigens for IHC. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, June 30, 2010 5:36 PM To: Schneider,Lynda S; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] Canine bone sectioning trouble Overall your fixation, decalcification and processing cycle is too short. The sample size is really big, why do you need it so thick? I would first of all let the samples fix longer 24 to 48 hours or I would trim them so that they are about 3-5 mm in thickness. Either way I like fixing bone samples for 24 to 48 hours. I'm not that accustomed to using RDO decal it is hydrochloric acid based and is quicker but even bone that size would take a while to decal. We normally use a formic acid based decal and a sample size that large my take up to 2 weeks to decal. Also to process a sample that size I would be at 4 to 6 hours a station. We frequently process porcine and goat knees in 2 x 3 blocks the samples are quite thick about a cm in thickness and we will process them at 4 to 6 hours a station. My recommendation would be to decal the larger cube of bone for a period of time until you can section through it. I would then cut a 3-4 mm thick piece off the cube and process it at 1 hour per station. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schneider,Lynda S Sent: Tuesday, June 29, 2010 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Canine bone sectioning trouble Hello out there... We are having trouble sectioning canine bone. The samples are bone marrow cubes approximately 2cm thick and 1in wide. They were fixed for 15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid decalcifier. They were faced in and then surface decaled again for about 30 mins. When sectioned, much of the marrow was missing and the bone was torn and shredded. We thought that maybe the samples had not been fixed sufficiently so refixed overnight in 10% NBF again. The samples were then reprocessed as they had been originally. The processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each, paraffin x 4, 40 mins each. Again they were faced in and decaled for another 30 mins or so. This time as soon as the sections are placed on the water bath (38 degrees) they explode and/or come apart so severely sections can almost not be picked up. If sections are even obtainable they are of horrible quality. Does anyone have any suggestions? Thank you so much in advance! Lynda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sun, 4 Jul 2010 10:57:39 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: Re: [Histonet] Unstained Slides To: "'Richard Cartun'" , , Message-ID: Content-Type: text/plain; charset="us-ascii" I do not bake my unstained slides for IHC controls and store them at 4dc. I bake them just before use. They do last longer this way but Richard is right, there is no telling because it all depends on the fixation, target protein stability and storage conditions. It is always a good idea to cut fresh sections if the stored unstained sections do not perform as expected. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, June 28, 2010 10:55 AM To: histonet@lists.utsouthwestern.edu; Rick.Garnhart@memorialhealthsystem.com Subject: SPAM-LOW: Re: [Histonet] Unstained Slides We bake our unstains at 60 degrees C. for 30 minutes prior to filing at RT. There is no set rule for stability. It all depends on fixation, the nature of the protein target, and storage conditions. I've seen absolutely spectacular immunoreactivity on unstained slides stored at RT for 20 years (and longer) and I've seen reduced immunoreactivity in unstained slides stored for as little as 4 weeks. We will always attempt to stain unstained slides when available; however, if the lesion or tumor is negative, and there is no internal control, you better cut fresh sections. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 6/28/2010 11:54 AM >>> Histoland, How is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 5 *************************************** From jeff.oliver <@t> asterand.com Tue Jul 6 08:22:11 2010 From: jeff.oliver <@t> asterand.com (Jeff Oliver) Date: Tue Jul 6 08:22:16 2010 Subject: [Histonet] Unsubscribe Message-ID: Unsubscribe please. Thanks, Jeff Oliver Services Lab Specialist II Asterand, Inc 440 Burroughs Detroit, Michigan 48202 phone: 313-263-0960 fax: 313-263-0961 From noreply+H29044536 <@t> netlogmail.com Tue Jul 6 08:58:53 2010 From: noreply+H29044536 <@t> netlogmail.com (=?UTF-8?B?5rKI5reR55C8?=) Date: Tue Jul 6 08:59:02 2010 Subject: [Histonet] =?utf-8?b?6K6/6Zeu5oiR55qETmV0bG9n5Liq5Lq65Li76aG1?= Message-ID: <0.0.8.B78.1CB1D135EA4A26C.0@mx01-3.netlogmail.com> ?, ?????????????????????????????????????????????????????????????????????????????????????????????????? ???: http://zh.netlog.com/go/mailurl/-bT0xMzQ2MDU0NDMmbD0xJmdtPTM3JnU9JTJGZ28lMkZyZWdpc3RlciUyRmlkJTNEOTk5OTU0NTk5JTI2aSUzRHQ5MQ__ ??, ??? ---------------------------------------------------------------- ?????????????????????? http://zh.netlog.com/go/mailurl/-bT0xMzQ2MDU0NDMmbD0yJmdtPTM3JnU9JTJGZ28lMkZub21haWxzJTJGaW52aXRlJTJGZW1haWwlM0QtYUdsemRHOXVaWFJBYkdsemRITXVkWFJ6YjNWMGFIZGxjM1JsY200dVpXUjElMjZjb2RlJTNEMTMzNTU0NTAlMjZpZCUzRDk5OTk1NDU5OSUyNmklM0R0OTI_ Don't want to receive invitations from your friends anymore? http://zh.netlog.com/go/mailurl/-bT0xMzQ2MDU0NDMmbD0zJmdtPTM3JnU9aHR0cCUzQSUyRiUyRmVuLm5ldGxvZy5jb20lMkZnbyUyRm5vbWFpbHMlMkZpbnZpdGUlMkZlbWFpbCUzRC1hR2x6ZEc5dVpYUkFiR2x6ZEhNdWRYUnpiM1YwYUhkbGMzUmxjbTR1WldSMSUyNmNvZGUlM0QxMzM1NTQ1MCUyNmlkJTNEOTk5OTU0NTk5JTI2aSUzRHQ5Mg__ From nancy_schmitt <@t> pa-ucl.com Tue Jul 6 10:14:23 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Jul 6 10:14:28 2010 Subject: [Histonet] universal microtome aligner tool Message-ID: <737BD0BF52F0744B96B74B61756AC06441E20E19AC@hestia.ad.pa-ucl.com> Has anyone used the universal microtome aligner tool from Newcomer Supply? It is universal and much less expensive than most I have seen. I am just looking for some feedback..... Thanks Nancy Schmitt HT, MLT (ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From baustin <@t> cbgbiotech.com Tue Jul 6 10:17:17 2010 From: baustin <@t> cbgbiotech.com (Beth Austin) Date: Tue Jul 6 10:18:02 2010 Subject: [Histonet] RE: Histonet- Xylene Substitutes Message-ID: Vanessa, I work with a lot of great histology folks and what I know about it is that eosin bleeding and blue-haze problems usually occur as a consequence of either not fully removing all of the water during the final absolute alcohol rinse step, and/or not fully removing all of the alcohol during the clearing step. If the water and alcohol are completely removed, bleeding and haze will not occur. I hope this helps. Beth Austin-Sell CBG Biotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: control tissue for Hall's bile stain (Robert Richmond) 2. RE: BILLING QUESTION (Cynthia Pyse) 3. RE: ER and PR validation (JMyers1@aol.com) 4. Thomas Crowell is out of the office. (thomas.crowell@novartis.com) 5. Looking for MIN6 cell line (Debra Baluch) 6. bat wing histology (Amos Brooks) 7. RE: BILLING QUESTION (Feher, Stephen) 8. xylene substitute (Vanessa Avalos) ---------------------------------------------------------------------- Message: 1 Date: Fri, 2 Jul 2010 13:48:52 -0400 From: Robert Richmond Subject: [Histonet] Re: control tissue for Hall's bile stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hall's bile stain - not really a stain but a reaction, like the Perls prussian blue reaction for iron - uses trichloracetic acid and ferric chloride to oxidize yellow-brown bilirubin to bright green biliverdin. Or so I've been told - I've never seen one. Something I've wondered about for a long time: could Hall's reaction be used to demonstrate meconium in macrophages in the chorion of the human placenta? These can be hard to see, particularly if you don't have a very good microscope. - If this would work, it would provide a ready source for controls, at least for the hospital pathologist. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 2 Date: Fri, 2 Jul 2010 13:51:51 -0400 From: "Cynthia Pyse" Subject: RE: [Histonet] BILLING QUESTION To: "'Sara Baldwin/mhhcc.org'" , Message-ID: <000001cb1a0f$40ca1c10$c25e5430$@com> Content-Type: text/plain; charset="us-ascii" We have a contract with the hospitals, this comes from our billing manger. We bill the hospitals, then they bill the patient. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Friday, July 02, 2010 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BILLING QUESTION Histonetters: If you do some work for another hospital (Histology) can you bill the hospital or do you have to bill the patient directly? Is there a statute or Regulation out there about this? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 2 Jul 2010 13:54:26 EDT From: JMyers1@aol.com Subject: RE: [Histonet] ER and PR validation To: histonet@lists.utsouthwestern.edu Cc: Dorothy.L.Webb@HealthPartners.Com Message-ID: <8f84.3cbd32f0.395f81d2@aol.com> Content-Type: text/plain; charset="US-ASCII" This paper can also be downloaded, indirectly, from the CAP's website: _http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_ (http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) Happy (re-)validating, Joe ------------------------------ Message: 12 Date: Fri, 2 Jul 2010 10:23:08 -0500 From: "Webb, Dorothy L" Subject: [Histonet] ER and PR validation To: _'histonet@lists.utsouthwestern.edu'_ (mailto:'histonet@lists.utsouthwestern.edu') In a recent article written by 4 pathologists, including Dr. Elizabeth Hammond and Dr. Patrick Fitzgibbons, the recommendations are now including testing with a lab that has validated it's assay against clinical outcomes. How is everyone planning on fulfilling this requirement inasmuch as we are not aware of any lab in our metro area with this kind of compliance. Thanks, as always, for any guidance in this area! If you would like a reprint of this article, email is pfitz@stjoe.org and the article is entitle "Recommendations for Validating Estrogen and Progesterone Receptor immunohistochemistry Assays". Dorothy Webb, HT Regions Histology Technical Supervisor ------------------------------ Message: 4 Date: Fri, 2 Jul 2010 15:32:28 -0400 From: thomas.crowell@novartis.com Subject: [Histonet] Thomas Crowell is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 07/02/2010 and will not return until 07/13/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. ------------------------------ Message: 5 Date: Fri, 2 Jul 2010 13:14:00 -0700 From: Debra Baluch Subject: [Histonet] Looking for MIN6 cell line To: "histonet@lists.utsouthwestern.edu" Message-ID: <80BBC916BEC29A498784913D007DD4A24DBDC0A1FD@EX10.asurite.ad.asu.edu> Content-Type: text/plain; charset="us-ascii" Does anyone know where I can obtain the MIN6 beta cell line derived from mouse pancreas? They are listed and used in many papers but do not list the source. I have tried ATCC and suggested cell banks in ECACC (England), DSMZ (Germany), and JCRB (Japan) but they are not listed. Thanks, Page Arizona State University/SoLS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMyers1@aol.com Sent: Friday, July 02, 2010 10:54 AM To: histonet@lists.utsouthwestern.edu Cc: Dorothy.L.Webb@HealthPartners.Com Subject: RE: [Histonet] ER and PR validation This paper can also be downloaded, indirectly, from the CAP's website: _http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_ (http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) Happy (re-)validating, Joe ------------------------------ Message: 12 Date: Fri, 2 Jul 2010 10:23:08 -0500 From: "Webb, Dorothy L" Subject: [Histonet] ER and PR validation To: _'histonet@lists.utsouthwestern.edu'_ (mailto:'histonet@lists.utsouthwestern.edu') In a recent article written by 4 pathologists, including Dr. Elizabeth Hammond and Dr. Patrick Fitzgibbons, the recommendations are now including testing with a lab that has validated it's assay against clinical outcomes. How is everyone planning on fulfilling this requirement inasmuch as we are not aware of any lab in our metro area with this kind of compliance. Thanks, as always, for any guidance in this area! If you would like a reprint of this article, email is pfitz@stjoe.org and the article is entitle "Recommendations for Validating Estrogen and Progesterone Receptor immunohistochemistry Assays". Dorothy Webb, HT Regions Histology Technical Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 2 Jul 2010 16:14:36 -0400 From: Amos Brooks Subject: [Histonet] bat wing histology To: mchadha1@gmail.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I do hope you are looking at cross sections of the wing and not the flat. That would be very difficult indeed. For good cross sections I would try a "Swiss Roll". This is a way of demonstrating a large amount of cross sectional area in small space. Take the membrane and fix it by submersion in the fixative of your choice. Prior to processing roll the whole membrane up then cut the membrane log into sections small enough to fit in a cassette. You can use foam biopsy pads to support this shape. Embed it and section it on edge to show a long coiled membrane. The hairs should be able to be displayed in this way as well. To show a lot of membrane at the same time you could place multiple rolls in one cassette. This should work well. Good Luck, Amos Message: 21 Date: Thu, 1 Jul 2010 11:46:17 -0400 From: Mohit Chadha Subject: [Histonet] bat wing histology To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello everyone, This is my very first post and I am desperately looking for help. I am new to histology, so any help would be much appreciated. I am studying the peripheral sensory innervation of bat wings. As a first step, I would like to demonstrate the innervation pattern on the different parts of the wing membrane (a whole mount of the wing?). Second, I would like to demonstrate the mechanoreceptor make-up of the tiny hairs on the wing membrane. Bat wings are highly elastic, with numerous folds, a thickness of about 35-45 microns (in the species I study), a network of thin collagen bundles, and pigmented superficial epidermal layers. I could provide more information if required. Hoping to hear back from the members. Thank you. ------------------------------ Message: 7 Date: Fri, 2 Jul 2010 17:18:14 -0400 From: "Feher, Stephen" Subject: RE: [Histonet] BILLING QUESTION To: "Cynthia Pyse" , "Sara Baldwin/mhhcc.org" , Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B75578@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" You can contract for services to another lab or hospital and depending upon the terms of the contract, you may be able to bill the patient. For example, you send all of your HER2's and ER/PR to another lab to be stained and quantified into a score. You may contract with the other lab for them to charge you a flat fee for the service and you will bill the patient for 88361x3. The other side is that you can contract for the other hospital to do the test, bill the patient and your hospital pays no fee but misses out on some of the compensation. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, July 02, 2010 1:52 PM To: 'Sara Baldwin/mhhcc.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BILLING QUESTION We have a contract with the hospitals, this comes from our billing manger. We bill the hospitals, then they bill the patient. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Friday, July 02, 2010 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BILLING QUESTION Histonetters: If you do some work for another hospital (Histology) can you bill the hospital or do you have to bill the patient directly? Is there a statute or Regulation out there about this? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 2 Jul 2010 15:20:52 -0700 From: "Vanessa Avalos" Subject: [Histonet] xylene substitute To: Message-ID: <000301cb1a34$dbdd5c10$93981430$@com> Content-Type: text/plain; charset="us-ascii" I am taking a chance that this is still a working email!! I came across an old post on histonet regarding Xylene subs with a linear stainer. I was wondering if you made the switch, and if so could you share the brand and your H&E protocol. I have had some issues with Eosin bleeding, slides very eosinophilic and blue haze on the slide. Not all at once but its enough to drive me crazy!! Your help would be appreciated. V.Avalos ADS, INC Fax:602-277-2134 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 4 *************************************** From KMB01 <@t> grh.org Tue Jul 6 10:50:26 2010 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Tue Jul 6 10:50:30 2010 Subject: [Histonet] universal microtome aligner tool In-Reply-To: <737BD0BF52F0744B96B74B61756AC06441E20E19AC@hestia.ad.pa-ucl.com> Message-ID: Yes, I just seen that too. Would like information too if anyone has any. Thanks Kathy Gorham H.T. Grande Ronde Hospital La Grande, Or 9780 GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, July 06, 2010 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] universal microtome aligner tool Has anyone used the universal microtome aligner tool from Newcomer Supply? It is universal and much less expensive than most I have seen. I am just looking for some feedback..... Thanks Nancy Schmitt HT, MLT (ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbass <@t> wfubmc.edu Tue Jul 6 14:49:58 2010 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Tue Jul 6 14:50:13 2010 Subject: [Histonet] Rnase free slides? In-Reply-To: Message-ID: Hello, I'm doing RNA work for the first time. My plan is to take a fresh rat brain, block quickly, freeze by immersing in dry-ice cooled isopentane, storing at -80, collecting tissue sections (thickness will be determined, somewhere between 20 and 300 um), and punching the particular regions I need out of the sections. I will then isolate RNA from the punches for qPCR analysis. Questions: 1) does this sound like a viable plan? 2) and suggestions, what to be careful of? 3) where do I have to be careful of Rnase, should I use disposable blades, cleaned with Rnase away? 4) where can I find Rnase free slides, or should I just make my own. I usually use charged slides. Any and all suggestions will be appreciated. I'm new to this and don't know where I will have problems. Thanks! Caroline From SohrabB1 <@t> ah.org Tue Jul 6 15:12:49 2010 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Tue Jul 6 15:13:41 2010 Subject: [Histonet] Gout Crytals Message-ID: <4C332BD0.4347.0054.1@ah.org> Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you From JWeems <@t> sjha.org Tue Jul 6 15:35:41 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jul 6 15:35:49 2010 Subject: [Histonet] Gout Crytals In-Reply-To: <4C332BD0.4347.0054.1@ah.org> References: <4C332BD0.4347.0054.1@ah.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015E968425@CHEXCMS10.one.ads.che.org> Should skip the formalin!!! They are water soluable... :>( -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Tuesday, July 06, 2010 16:13 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From JMacDonald <@t> mtsac.edu Tue Jul 6 15:50:19 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jul 6 15:50:27 2010 Subject: [Histonet] Gout Crytals In-Reply-To: <4C332BD0.4347.0054.1@ah.org> Message-ID: Uric acid crystals are water soluble. Avoid formalin and fix in absolute alcohol and start the processing of the tissue in absolute alcohol. Jennifer "Behnaz Sohrab" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 01:28 PM To cc Subject [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Tue Jul 6 15:55:24 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Jul 6 15:55:29 2010 Subject: [Histonet] Gout Crytals Message-ID: <20100706135524.9e2d9aa830e8449a2412eb1e4f2f067e.8ce03ce3da.wbe@email04.secureserver.net> If you have fixed in formalin the gout crystals are all gone!!!!& nbsp; Have to start over with a new sample if possible. The crystals Sarah Goebel, B.A. Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, (512)386-5107 -------- Original Message -------- Subject: [Histonet] Gout Crytals From: "Behnaz Sohrab" <[1]SohrabB1@ah. Date: Tue, July 06, 2010 1:12 pm To: <[2]histonet@lis Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alc Thank you _______________________________________________ Histonet mailing list [3]Histonet@lists.utsou [4]http: References 1. 3D"mailto://SohrabB1@ah.org"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Sandy.Schmitz <@t> leica-microsystems.com Tue Jul 6 16:00:56 2010 From: Sandy.Schmitz <@t> leica-microsystems.com (Sandy.Schmitz@leica-microsystems.com) Date: Tue Jul 6 16:01:05 2010 Subject: [Histonet] Schmitz, Sandy is out of the office. Message-ID: I will be out of the office starting 07/05/2010 and will not return until 07/07/2010. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Joyce.Cline <@t> wchsys.org Tue Jul 6 15:56:52 2010 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Tue Jul 6 16:10:18 2010 Subject: [Histonet] Gout Crytals In-Reply-To: <4C332BD0.4347.0054.1@ah.org> References: <4C332BD0.4347.0054.1@ah.org> Message-ID: This works for us. Process normally, we usually fix in 100% alcohol. Cut slide Formula 83 or Xylene 20 seconds Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds Eosin for 20 seconds 100% alcohol 20 seconds 100% alcohol 20 seconds Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds Form 83 or Xylene for 20 seconds coverslip normally Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab [SohrabB1@ah.org] Sent: Tuesday, July 06, 2010 4:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From mchadha1 <@t> gmail.com Tue Jul 6 16:10:17 2010 From: mchadha1 <@t> gmail.com (Mohit Chadha) Date: Tue Jul 6 16:10:24 2010 Subject: [Histonet] bat wing histology In-Reply-To: References: Message-ID: Thank you everyone for replying, much appreciated. Having also talked to people in my dept, I have a rudimentary protocol ready. Of course, I will have to tweak it to see what works. I will be using anti PGP9.5 antibody for neuronal immunology. I am still not sure how to section the wing. In most likelihood, I will be using freezing sliding microtome. The "swiss roll" method sound good and I will definitely try it. I am thinking that since the wing membrane is thin (~30 um), I will also try to use the whole mount of small pieces. Any other thoughts and advice would be appreciated. Thank you, Mohit Chadha, Univ of Maryland. On Fri, Jul 2, 2010 at 4:14 PM, Amos Brooks wrote: > Hi, > I do hope you are looking at cross sections of the wing and not the > flat. That would be very difficult indeed. For good cross sections I would > try a "Swiss Roll". This is a way of demonstrating a large amount of cross > sectional area in small space. Take the membrane and fix it by submersion in > the fixative of your choice. Prior to processing roll the whole membrane up > then cut the membrane log into sections small enough to fit in a cassette. > You can use foam biopsy pads to support this shape. Embed it and section it > on edge to show a long coiled membrane. The hairs should be able to be > displayed in this way as well. To show a lot of membrane at the same time > you could place multiple rolls in one cassette. This should work well. > > Good Luck, > Amos > > > Message: 21 > Date: Thu, 1 Jul 2010 11:46:17 -0400 > From: Mohit Chadha > Subject: [Histonet] bat wing histology > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello everyone, > > This is my very first post and I am desperately looking for help. I am new > to histology, so any help would be much appreciated. > > I am studying the peripheral sensory innervation of bat wings. As a first > step, I would like to demonstrate the innervation pattern on the different > parts of the wing membrane (a whole mount of the wing?). Second, I would > like to demonstrate the mechanoreceptor make-up of the tiny hairs on the > wing membrane. > > Bat wings are highly elastic, with numerous folds, a thickness of about > 35-45 microns (in the species I study), a network of thin collagen bundles, > and pigmented superficial epidermal layers. > I could provide more information if required. > > Hoping to hear back from the members. > Thank you. > From jdouglas <@t> mdanderson.org Tue Jul 6 16:22:46 2010 From: jdouglas <@t> mdanderson.org (Douglas,Joseph) Date: Tue Jul 6 16:22:55 2010 Subject: [Histonet] Gout Crytals In-Reply-To: References: <4C332BD0.4347.0054.1@ah.org> Message-ID: REMOVE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, July 06, 2010 3:50 PM To: Behnaz Sohrab Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Gout Crytals Uric acid crystals are water soluble. Avoid formalin and fix in absolute alcohol and start the processing of the tissue in absolute alcohol. Jennifer "Behnaz Sohrab" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 01:28 PM To cc Subject [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dcojita <@t> tampabay.rr.com Tue Jul 6 17:29:09 2010 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Tue Jul 6 17:29:14 2010 Subject: [Histonet] used histology equipment Message-ID: Does anyone know of a reputable dealer for used equipment? From 41dmb41 <@t> gmail.com Tue Jul 6 17:55:53 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Tue Jul 6 17:56:19 2010 Subject: [Histonet] used histology equipment In-Reply-To: References: Message-ID: Absolutely... Southeast Pathology Instrument Service out of Charleston, SC. The owner's name is Michael Dietrich. I've done business with him before and they are great people, very honest and they stand behind their instruments. I would highly recommend them to anyone. Contact Michael directly and tell him Drew Meyer from Atlanta referred you. http://southeastpathology.com/ Drew On Tue, Jul 6, 2010 at 18:29, wrote: > Does anyone know of a reputable dealer for used equipment? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Tue Jul 6 18:24:44 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jul 6 18:24:49 2010 Subject: [Histonet] Rnase free slides? In-Reply-To: References: Message-ID: Honestly, I think RNases are a bunch of hooha. If you're being careful anyway because you're doing a PCR, that should be enough. Wear gloves, be sterile. When I worked with a Russian post-doc, she said she did RNA in situ hybridization without gloves and it worked. Of course, god only knows what her protocol was, as she had some crazy stories about Russian labs. Emily -- Dark Pictures, thrones and stones that pilgrims kiss And poems that take a thousand years to die But ape the immortality of this Red label on a little butterfly. -Vladimir Nabokov, concluding stanza of ?A Discovery? 1941. On Tue, Jul 6, 2010 at 3:49 PM, Caroline Bass wrote: > Hello, > > I'm doing RNA work for the first time. My plan is to take a fresh rat brain, > block quickly, freeze by immersing in dry-ice cooled isopentane, storing at > -80, collecting tissue sections (thickness will be determined, somewhere > between 20 and 300 um), and punching the particular regions I need out of > the sections. I will then isolate RNA from the punches for qPCR analysis. > > Questions: > > 1) does this sound like a viable plan? > 2) and suggestions, what to be careful of? > 3) where do I have to be careful of Rnase, should I use disposable blades, > cleaned with Rnase away? > 4) where can I find Rnase free slides, or should I just make my own. I > usually use charged slides. > > Any and all suggestions will be appreciated. I'm new to this and don't know > where I will have problems. > > Thanks! > > Caroline > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Tue Jul 6 21:10:44 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Jul 6 21:10:48 2010 Subject: [Histonet] bat wing histology In-Reply-To: References: Message-ID: Hi, We did this on derm punch biopsies for a while. We used PLP (paraformaldehyde-lysine periodate) fixative. This was also done on a sliding microtome at 50um with piles of dry ice to keep the sucrose frozen. It was a bit of a pain in the butt, but we got decent results. I think the sectioning process could have been easier, but the PI was obsessed with not modifying the project at all, even if it meant improvements. We stained these as floating sections in 96 well plates, so in this case I would be concerned that the wing rolls would un-roll if you were to do the swiss roll thing I described. Have fun :-) Amos On Tue, Jul 6, 2010 at 5:10 PM, Mohit Chadha wrote: > Thank you everyone for replying, much appreciated. > > Having also talked to people in my dept, I have a rudimentary protocol > ready. Of course, I will have to tweak it to see what works. I will be using > anti PGP9.5 antibody for neuronal immunology. > > I am still not sure how to section the wing. In most likelihood, I will be > using freezing sliding microtome. The "swiss roll" method sound good and I > will definitely try it. I am thinking that since the wing membrane is thin > (~30 um), I will also try to use the whole mount of small pieces. > > Any other thoughts and advice would be appreciated. > > Thank you, > Mohit Chadha, > Univ of Maryland. > > > > > > > > On Fri, Jul 2, 2010 at 4:14 PM, Amos Brooks wrote: > >> Hi, >> I do hope you are looking at cross sections of the wing and not the >> flat. That would be very difficult indeed. For good cross sections I would >> try a "Swiss Roll". This is a way of demonstrating a large amount of cross >> sectional area in small space. Take the membrane and fix it by submersion in >> the fixative of your choice. Prior to processing roll the whole membrane up >> then cut the membrane log into sections small enough to fit in a cassette. >> You can use foam biopsy pads to support this shape. Embed it and section it >> on edge to show a long coiled membrane. The hairs should be able to be >> displayed in this way as well. To show a lot of membrane at the same time >> you could place multiple rolls in one cassette. This should work well. >> >> Good Luck, >> Amos >> >> >> Message: 21 >> Date: Thu, 1 Jul 2010 11:46:17 -0400 >> From: Mohit Chadha >> Subject: [Histonet] bat wing histology >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hello everyone, >> >> This is my very first post and I am desperately looking for help. I am new >> to histology, so any help would be much appreciated. >> >> I am studying the peripheral sensory innervation of bat wings. As a first >> step, I would like to demonstrate the innervation pattern on the different >> parts of the wing membrane (a whole mount of the wing?). Second, I would >> like to demonstrate the mechanoreceptor make-up of the tiny hairs on the >> wing membrane. >> >> Bat wings are highly elastic, with numerous folds, a thickness of about >> 35-45 microns (in the species I study), a network of thin collagen >> bundles, >> and pigmented superficial epidermal layers. >> I could provide more information if required. >> >> Hoping to hear back from the members. >> Thank you. >> > > From jcampbell <@t> vdxpathology.com Tue Jul 6 21:11:14 2010 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Tue Jul 6 21:11:18 2010 Subject: [Histonet] Has anyone used a biotin block in their antibody diluent? Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FE1@VDXSERVER01.vdxpathology.local> Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell From j.h.reilly <@t> clinmed.gla.ac.uk Wed Jul 7 03:05:17 2010 From: j.h.reilly <@t> clinmed.gla.ac.uk (Jim Reilly) Date: Wed Jul 7 03:05:27 2010 Subject: [Histonet] Biotin block Message-ID: Hello Jennifer I use the Avidin/Biotin blocking kit from Vector SP-2001 I mix the Avidin D with my normal blocking serum and the biotin I add to my primary antibody diluent. This seems to work well for most tissue types. Cheers Jim From MAUGER <@t> email.chop.edu Wed Jul 7 06:32:52 2010 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Wed Jul 7 06:34:29 2010 Subject: [Histonet] RE: Has anyone used a biotin block in their antibody diluent? In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF8181FE1@VDXSERVER01.vdxpathology.local> References: <5658CBDB9EAE6545ABE50D2563D81BF8181FE1@VDXSERVER01.vdxpathology.local> Message-ID: <443F5B475A9BF647AB962E834884EBAD278D1277FE@EX7CCRPW03V1.chop.edu> Jennifer, Vector has an avidin-biotin blocking kit that works well by itself or mixed with reagents- very reasonable cost. Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell [jcampbell@vdxpathology.com] Sent: Tuesday, July 06, 2010 10:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Has anyone used a biotin block in their antibody diluent? Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Wed Jul 7 08:37:27 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Jul 7 08:37:56 2010 Subject: [Histonet] used histology equipment In-Reply-To: Message-ID: Hi Diane, Because of your location I would recommend Micro-optics of Florida. Mike Jones or Tom Christy. The number is 954-791-0082. They are great people to work with and I have found them very reasonable on their prices. Hope this helps! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 05:29 PM To "'histonet'" cc Subject [Histonet] used histology equipment Does anyone know of a reputable dealer for used equipment? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From jdouglas <@t> mdanderson.org Wed Jul 7 08:40:37 2010 From: jdouglas <@t> mdanderson.org (Douglas,Joseph) Date: Wed Jul 7 08:40:45 2010 Subject: [Histonet] used histology equipment In-Reply-To: References: Message-ID: REMOVE FROM DATABASE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, July 06, 2010 5:56 PM To: dcojita@tampabay.rr.com Cc: histonet Subject: Re: [Histonet] used histology equipment Absolutely... Southeast Pathology Instrument Service out of Charleston, SC. The owner's name is Michael Dietrich. I've done business with him before and they are great people, very honest and they stand behind their instruments. I would highly recommend them to anyone. Contact Michael directly and tell him Drew Meyer from Atlanta referred you. http://southeastpathology.com/ Drew On Tue, Jul 6, 2010 at 18:29, wrote: > Does anyone know of a reputable dealer for used equipment? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdouglas <@t> mdanderson.org Wed Jul 7 08:41:59 2010 From: jdouglas <@t> mdanderson.org (Douglas,Joseph) Date: Wed Jul 7 08:42:03 2010 Subject: [Histonet] used histology equipment In-Reply-To: References: Message-ID: REMOVE FROM DATABASE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, July 07, 2010 8:37 AM To: dcojita@tampabay.rr.com Cc: 'histonet'; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] used histology equipment Hi Diane, Because of your location I would recommend Micro-optics of Florida. Mike Jones or Tom Christy. The number is 954-791-0082. They are great people to work with and I have found them very reasonable on their prices. Hope this helps! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 05:29 PM To "'histonet'" cc Subject [Histonet] used histology equipment Does anyone know of a reputable dealer for used equipment? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Wed Jul 7 10:36:47 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Jul 7 10:36:53 2010 Subject: [Histonet] Reprocess Message-ID: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> Hello all!! Hope everyone had a happy 4th!! Question grossed in some fat that I need to routine process. I ha this in the past with extra fixation and no problem? This tim however it didn't fix all the way through and I have oily unfixed fat in section we longer and rep then what? older =) Thanks in advance!! Sarah Goebel, B.A., HT ( Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 From catherinesimonson <@t> gmail.com Wed Jul 7 10:47:39 2010 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Wed Jul 7 10:47:47 2010 Subject: [Histonet] Reprocess In-Reply-To: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> References: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> Message-ID: Easy option is to melt down, and run cassettes through the clean cycle on your processors to remove all the paraffin. then process as usual. I've done this before with decent results. Good luck! Catherine On Wed, Jul 7, 2010 at 11:36 AM, wrote: > > Hello all!! Hope everyone had a happy 4th!! Question today is...I > grossed in some fat that I need to routine process. I ha ve done > this in the past with extra fixation and no problem? This tim e > however it didn't fix all the way through and I have oily unfixed fat > in the middle of my block. I fixed for 48 hours, but guess my > section we re too big and needed more. I want to fix for a little > longer and rep rocess the block. I know I need to melt it down, but > then what? I have done this before, it's just my brain is getting > older =) > > Thanks in advance!! > > Sarah Goebel, B.A., HT ( ASCP) > > Histotechnician > > XBiotech USA Inc. > > > 8201 East Riverside Dr. Bldg 4 Suite 100 > > Austin, Texas 78744 > > (512)386-5107 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cmiller <@t> physlab.com Wed Jul 7 10:53:18 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jul 7 10:53:24 2010 Subject: [Histonet] Reprocess In-Reply-To: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> References: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> Message-ID: Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run them through the cleaning cycle on my processor. The Xylene and Alcohol will melt the oily fat. When the clean cycle is complete I just drop them back into formalin until I process that evening. It works very well. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cmiller <@t> physlab.com Wed Jul 7 11:01:37 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jul 7 11:01:43 2010 Subject: [Histonet] Reprocess In-Reply-To: References: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> Message-ID: I take that back I do melt them, recap the cassettes and then drop them in to clean. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, July 07, 2010 10:53 AM To: sgoebel@xbiotech.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocess Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run them through the cleaning cycle on my processor. The Xylene and Alcohol will melt the oily fat. When the clean cycle is complete I just drop them back into formalin until I process that evening. It works very well. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Janice.Mahoney <@t> alegent.org Wed Jul 7 11:24:51 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Jul 7 11:24:59 2010 Subject: [Histonet] Reprocess In-Reply-To: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> References: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2F0@EXCHMBC2.ad.ah.local> Cheri's process is a good one, we use it too. Works every time. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Allison_Scott <@t> hchd.tmc.edu Wed Jul 7 11:26:12 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Jul 7 11:26:18 2010 Subject: [Histonet] QC log Message-ID: <1872B4A455B7974391609AD8034C79FC026DFC8E@LBEXCH01.hchd.local> Hello to all in histoland. Does anyone have a QC log sheet that documents the H&E stain, microtomy, floaters and other issues that they would be willing to share with me. Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From HOOD <@t> tarleton.edu Wed Jul 7 11:39:05 2010 From: HOOD <@t> tarleton.edu (HOOD MS. GLENDA) Date: Wed Jul 7 11:39:14 2010 Subject: [Histonet] RE: gout crystals In-Reply-To: <01NP77QHBPD48WYDX4@vms.tarleton.edu> References: <01NP77QHBPD48WYDX4@vms.tarleton.edu> Message-ID: It is true that gout crystals are water-soluble, and we are all taught that... but a few years ago there was a published article (JOH??) that showed that many crystals DO survive after water-based fixation. Since you already have the specimen in formalin, at least try processing and see if they can be demonstrated. Couldn't hurt, not nearly as much as the patient would for a re-biopsy! Glenda F. Hood, M.Ed., HT(ASCP) Instructor and Program Director Histotechnology Program Tarleton State University 817-926-1101 x6 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 07, 2010 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Rnase free slides? (Caroline Bass) 2. Gout Crytals (Behnaz Sohrab) 3. RE: Gout Crytals (Weems, Joyce) 4. Re: Gout Crytals (Jennifer MacDonald) 5. RE: Gout Crytals (sgoebel@xbiotech.com) 6. Schmitz, Sandy is out of the office. (Sandy.Schmitz@leica-microsystems.com) 7. RE: Gout Crytals (Joyce Cline) 8. Re: bat wing histology (Mohit Chadha) 9. RE: Gout Crytals (Douglas,Joseph) 10. used histology equipment (dcojita@tampabay.rr.com) 11. Re: used histology equipment (Drew Meyer) 12. Re: Rnase free slides? (Emily Sours) 13. Re: bat wing histology (Amos Brooks) 14. Has anyone used a biotin block in their antibody diluent? (Jennifer Campbell) 15. Biotin block (Jim Reilly) 16. RE: Has anyone used a biotin block in their antibody diluent? (Mauger, Joanne) 17. Re: used histology equipment (Kim.Donadio@bhcpns.org) 18. RE: used histology equipment (Douglas,Joseph) 19. RE: used histology equipment (Douglas,Joseph) 20. Reprocess (sgoebel@xbiotech.com) 21. Re: Reprocess (Catherine Simonson) 22. RE: Reprocess (Cheri Miller) 23. RE: Reprocess (Cheri Miller) 24. RE: Reprocess (Mahoney,Janice A) ---------------------------------------------------------------------- Message: 1 Date: Tue, 06 Jul 2010 15:49:58 -0400 From: Caroline Bass Subject: [Histonet] Rnase free slides? To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello, I'm doing RNA work for the first time. My plan is to take a fresh rat brain, block quickly, freeze by immersing in dry-ice cooled isopentane, storing at -80, collecting tissue sections (thickness will be determined, somewhere between 20 and 300 um), and punching the particular regions I need out of the sections. I will then isolate RNA from the punches for qPCR analysis. Questions: 1) does this sound like a viable plan? 2) and suggestions, what to be careful of? 3) where do I have to be careful of Rnase, should I use disposable blades, cleaned with Rnase away? 4) where can I find Rnase free slides, or should I just make my own. I usually use charged slides. Any and all suggestions will be appreciated. I'm new to this and don't know where I will have problems. Thanks! Caroline ------------------------------ Message: 2 Date: Tue, 06 Jul 2010 13:12:49 -0700 From: "Behnaz Sohrab" Subject: [Histonet] Gout Crytals To: Message-ID: <4C332BD0.4347.0054.1@ah.org> Content-Type: text/plain; charset=US-ASCII Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you ------------------------------ Message: 3 Date: Tue, 6 Jul 2010 16:35:41 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Gout Crytals To: Behnaz Sohrab , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015E968425@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Should skip the formalin!!! They are water soluable... :>( -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Tuesday, July 06, 2010 16:13 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Tue, 6 Jul 2010 13:50:19 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Gout Crytals To: "Behnaz Sohrab" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Uric acid crystals are water soluble. Avoid formalin and fix in absolute alcohol and start the processing of the tissue in absolute alcohol. Jennifer "Behnaz Sohrab" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 01:28 PM To cc Subject [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 06 Jul 2010 13:55:24 -0700 From: sgoebel@xbiotech.com Subject: RE: [Histonet] Gout Crytals To: "Behnaz Sohrab" Cc: histonet@lists.utsouthwestern.edu Message-ID: <20100706135524.9e2d9aa830e8449a2412eb1e4f2f067e.8ce03ce3da.wbe@email04.secureserver.net> Content-Type: text/plain; charset="utf-8" If you have fixed in formalin the gout crystals are all gone!!!!& nbsp; Have to start over with a new sample if possible. The crystals Sarah Goebel, B.A. Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, (512)386-5107 -------- Original Message -------- Subject: [Histonet] Gout Crytals From: "Behnaz Sohrab" <[1]SohrabB1@ah. Date: Tue, July 06, 2010 1:12 pm To: <[2]histonet@lis Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alc Thank you _______________________________________________ Histonet mailing list [3]Histonet@lists.utsou [4]http: References 1. 3D"mailto://SohrabB1@ah.org"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" ------------------------------ Message: 6 Date: Tue, 6 Jul 2010 16:00:56 -0500 From: Sandy.Schmitz@leica-microsystems.com Subject: [Histonet] Schmitz, Sandy is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 07/05/2010 and will not return until 07/07/2010. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 7 Date: Tue, 6 Jul 2010 16:56:52 -0400 From: Joyce Cline Subject: RE: [Histonet] Gout Crytals To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" This works for us. Process normally, we usually fix in 100% alcohol. Cut slide Formula 83 or Xylene 20 seconds Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds Eosin for 20 seconds 100% alcohol 20 seconds 100% alcohol 20 seconds Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds Form 83 or Xylene for 20 seconds coverslip normally Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab [SohrabB1@ah.org] Sent: Tuesday, July 06, 2010 4:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 8 Date: Tue, 6 Jul 2010 17:10:17 -0400 From: Mohit Chadha Subject: Re: [Histonet] bat wing histology To: Amos Brooks Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Thank you everyone for replying, much appreciated. Having also talked to people in my dept, I have a rudimentary protocol ready. Of course, I will have to tweak it to see what works. I will be using anti PGP9.5 antibody for neuronal immunology. I am still not sure how to section the wing. In most likelihood, I will be using freezing sliding microtome. The "swiss roll" method sound good and I will definitely try it. I am thinking that since the wing membrane is thin (~30 um), I will also try to use the whole mount of small pieces. Any other thoughts and advice would be appreciated. Thank you, Mohit Chadha, Univ of Maryland. On Fri, Jul 2, 2010 at 4:14 PM, Amos Brooks wrote: > Hi, > I do hope you are looking at cross sections of the wing and not the > flat. That would be very difficult indeed. For good cross sections I would > try a "Swiss Roll". This is a way of demonstrating a large amount of cross > sectional area in small space. Take the membrane and fix it by submersion in > the fixative of your choice. Prior to processing roll the whole membrane up > then cut the membrane log into sections small enough to fit in a cassette. > You can use foam biopsy pads to support this shape. Embed it and section it > on edge to show a long coiled membrane. The hairs should be able to be > displayed in this way as well. To show a lot of membrane at the same time > you could place multiple rolls in one cassette. This should work well. > > Good Luck, > Amos > > > Message: 21 > Date: Thu, 1 Jul 2010 11:46:17 -0400 > From: Mohit Chadha > Subject: [Histonet] bat wing histology > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello everyone, > > This is my very first post and I am desperately looking for help. I am new > to histology, so any help would be much appreciated. > > I am studying the peripheral sensory innervation of bat wings. As a first > step, I would like to demonstrate the innervation pattern on the different > parts of the wing membrane (a whole mount of the wing?). Second, I would > like to demonstrate the mechanoreceptor make-up of the tiny hairs on the > wing membrane. > > Bat wings are highly elastic, with numerous folds, a thickness of about > 35-45 microns (in the species I study), a network of thin collagen bundles, > and pigmented superficial epidermal layers. > I could provide more information if required. > > Hoping to hear back from the members. > Thank you. > ------------------------------ Message: 9 Date: Tue, 6 Jul 2010 16:22:46 -0500 From: "Douglas,Joseph" Subject: RE: [Histonet] Gout Crytals To: 'Jennifer MacDonald' , Behnaz Sohrab Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" REMOVE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, July 06, 2010 3:50 PM To: Behnaz Sohrab Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Gout Crytals Uric acid crystals are water soluble. Avoid formalin and fix in absolute alcohol and start the processing of the tissue in absolute alcohol. Jennifer "Behnaz Sohrab" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 01:28 PM To cc Subject [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 6 Jul 2010 18:29:09 -0400 From: Subject: [Histonet] used histology equipment To: "'histonet'" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone know of a reputable dealer for used equipment? ------------------------------ Message: 11 Date: Tue, 6 Jul 2010 18:55:53 -0400 From: Drew Meyer <41dmb41@gmail.com> Subject: Re: [Histonet] used histology equipment To: dcojita@tampabay.rr.com Cc: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Absolutely... Southeast Pathology Instrument Service out of Charleston, SC. The owner's name is Michael Dietrich. I've done business with him before and they are great people, very honest and they stand behind their instruments. I would highly recommend them to anyone. Contact Michael directly and tell him Drew Meyer from Atlanta referred you. http://southeastpathology.com/ Drew On Tue, Jul 6, 2010 at 18:29, wrote: > Does anyone know of a reputable dealer for used equipment? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Tue, 6 Jul 2010 19:24:44 -0400 From: Emily Sours Subject: Re: [Histonet] Rnase free slides? To: Caroline Bass , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Honestly, I think RNases are a bunch of hooha. If you're being careful anyway because you're doing a PCR, that should be enough. Wear gloves, be sterile. When I worked with a Russian post-doc, she said she did RNA in situ hybridization without gloves and it worked. Of course, god only knows what her protocol was, as she had some crazy stories about Russian labs. Emily -- Dark Pictures, thrones and stones that pilgrims kiss And poems that take a thousand years to die But ape the immortality of this Red label on a little butterfly. -Vladimir Nabokov, concluding stanza of ???A Discovery??? 1941. On Tue, Jul 6, 2010 at 3:49 PM, Caroline Bass wrote: > Hello, > > I'm doing RNA work for the first time. My plan is to take a fresh rat brain, > block quickly, freeze by immersing in dry-ice cooled isopentane, storing at > -80, collecting tissue sections (thickness will be determined, somewhere > between 20 and 300 um), and punching the particular regions I need out of > the sections. I will then isolate RNA from the punches for qPCR analysis. > > Questions: > > 1) does this sound like a viable plan? > 2) and suggestions, what to be careful of? > 3) where do I have to be careful of Rnase, should I use disposable blades, > cleaned with Rnase away? > 4) where can I find Rnase free slides, or should I just make my own. I > usually use charged slides. > > Any and all suggestions will be appreciated. I'm new to this and don't know > where I will have problems. > > Thanks! > > Caroline > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Tue, 6 Jul 2010 22:10:44 -0400 From: Amos Brooks Subject: Re: [Histonet] bat wing histology To: Mohit Chadha Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, We did this on derm punch biopsies for a while. We used PLP (paraformaldehyde-lysine periodate) fixative. This was also done on a sliding microtome at 50um with piles of dry ice to keep the sucrose frozen. It was a bit of a pain in the butt, but we got decent results. I think the sectioning process could have been easier, but the PI was obsessed with not modifying the project at all, even if it meant improvements. We stained these as floating sections in 96 well plates, so in this case I would be concerned that the wing rolls would un-roll if you were to do the swiss roll thing I described. Have fun :-) Amos On Tue, Jul 6, 2010 at 5:10 PM, Mohit Chadha wrote: > Thank you everyone for replying, much appreciated. > > Having also talked to people in my dept, I have a rudimentary protocol > ready. Of course, I will have to tweak it to see what works. I will be using > anti PGP9.5 antibody for neuronal immunology. > > I am still not sure how to section the wing. In most likelihood, I will be > using freezing sliding microtome. The "swiss roll" method sound good and I > will definitely try it. I am thinking that since the wing membrane is thin > (~30 um), I will also try to use the whole mount of small pieces. > > Any other thoughts and advice would be appreciated. > > Thank you, > Mohit Chadha, > Univ of Maryland. > > > > > > > > On Fri, Jul 2, 2010 at 4:14 PM, Amos Brooks wrote: > >> Hi, >> I do hope you are looking at cross sections of the wing and not the >> flat. That would be very difficult indeed. For good cross sections I would >> try a "Swiss Roll". This is a way of demonstrating a large amount of cross >> sectional area in small space. Take the membrane and fix it by submersion in >> the fixative of your choice. Prior to processing roll the whole membrane up >> then cut the membrane log into sections small enough to fit in a cassette. >> You can use foam biopsy pads to support this shape. Embed it and section it >> on edge to show a long coiled membrane. The hairs should be able to be >> displayed in this way as well. To show a lot of membrane at the same time >> you could place multiple rolls in one cassette. This should work well. >> >> Good Luck, >> Amos >> >> >> Message: 21 >> Date: Thu, 1 Jul 2010 11:46:17 -0400 >> From: Mohit Chadha >> Subject: [Histonet] bat wing histology >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hello everyone, >> >> This is my very first post and I am desperately looking for help. I am new >> to histology, so any help would be much appreciated. >> >> I am studying the peripheral sensory innervation of bat wings. As a first >> step, I would like to demonstrate the innervation pattern on the different >> parts of the wing membrane (a whole mount of the wing?). Second, I would >> like to demonstrate the mechanoreceptor make-up of the tiny hairs on the >> wing membrane. >> >> Bat wings are highly elastic, with numerous folds, a thickness of about >> 35-45 microns (in the species I study), a network of thin collagen >> bundles, >> and pigmented superficial epidermal layers. >> I could provide more information if required. >> >> Hoping to hear back from the members. >> Thank you. >> > > ------------------------------ Message: 14 Date: Tue, 6 Jul 2010 19:11:14 -0700 From: "Jennifer Campbell" Subject: [Histonet] Has anyone used a biotin block in their antibody diluent? To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FE1@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="iso-8859-1" Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell ------------------------------ Message: 15 Date: Wed, 7 Jul 2010 09:05:17 +0100 From: "Jim Reilly" Subject: [Histonet] Biotin block To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Jennifer I use the Avidin/Biotin blocking kit from Vector SP-2001 I mix the Avidin D with my normal blocking serum and the biotin I add to my primary antibody diluent. This seems to work well for most tissue types. Cheers Jim ------------------------------ Message: 16 Date: Wed, 7 Jul 2010 07:32:52 -0400 From: "Mauger, Joanne" Subject: [Histonet] RE: Has anyone used a biotin block in their antibody diluent? To: Jennifer Campbell , "histonet@lists.utsouthwestern.edu" Message-ID: <443F5B475A9BF647AB962E834884EBAD278D1277FE@EX7CCRPW03V1.chop.edu> Content-Type: text/plain; charset="us-ascii" Jennifer, Vector has an avidin-biotin blocking kit that works well by itself or mixed with reagents- very reasonable cost. Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell [jcampbell@vdxpathology.com] Sent: Tuesday, July 06, 2010 10:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Has anyone used a biotin block in their antibody diluent? Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 7 Jul 2010 08:37:27 -0500 From: Kim.Donadio@bhcpns.org Subject: Re: [Histonet] used histology equipment To: Cc: 'histonet' , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi Diane, Because of your location I would recommend Micro-optics of Florida. Mike Jones or Tom Christy. The number is 954-791-0082. They are great people to work with and I have found them very reasonable on their prices. Hope this helps! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 05:29 PM To "'histonet'" cc Subject [Histonet] used histology equipment Does anyone know of a reputable dealer for used equipment? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 18 Date: Wed, 7 Jul 2010 08:40:37 -0500 From: "Douglas,Joseph" Subject: RE: [Histonet] used histology equipment To: 'Drew Meyer' <41dmb41@gmail.com>, "dcojita@tampabay.rr.com" Cc: histonet Message-ID: Content-Type: text/plain; charset="us-ascii" REMOVE FROM DATABASE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, July 06, 2010 5:56 PM To: dcojita@tampabay.rr.com Cc: histonet Subject: Re: [Histonet] used histology equipment Absolutely... Southeast Pathology Instrument Service out of Charleston, SC. The owner's name is Michael Dietrich. I've done business with him before and they are great people, very honest and they stand behind their instruments. I would highly recommend them to anyone. Contact Michael directly and tell him Drew Meyer from Atlanta referred you. http://southeastpathology.com/ Drew On Tue, Jul 6, 2010 at 18:29, wrote: > Does anyone know of a reputable dealer for used equipment? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 7 Jul 2010 08:41:59 -0500 From: "Douglas,Joseph" Subject: RE: [Histonet] used histology equipment To: "'Kim.Donadio@bhcpns.org'" , "dcojita@tampabay.rr.com" Cc: 'histonet' , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" REMOVE FROM DATABASE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, July 07, 2010 8:37 AM To: dcojita@tampabay.rr.com Cc: 'histonet'; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] used histology equipment Hi Diane, Because of your location I would recommend Micro-optics of Florida. Mike Jones or Tom Christy. The number is 954-791-0082. They are great people to work with and I have found them very reasonable on their prices. Hope this helps! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 05:29 PM To "'histonet'" cc Subject [Histonet] used histology equipment Does anyone know of a reputable dealer for used equipment? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. 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Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 07 Jul 2010 08:36:47 -0700 From: sgoebel@xbiotech.com Subject: [Histonet] Reprocess To: histonet@lists.utsouthwestern.edu Message-ID: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> Content-Type: text/plain; charset="utf-8" Hello all!! Hope everyone had a happy 4th!! Question grossed in some fat that I need to routine process. I ha this in the past with extra fixation and no problem? This tim however it didn't fix all the way through and I have oily unfixed fat in section we longer and rep then what? older =) Thanks in advance!! Sarah Goebel, B.A., HT ( Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 ------------------------------ Message: 21 Date: Wed, 7 Jul 2010 11:47:39 -0400 From: Catherine Simonson Subject: Re: [Histonet] Reprocess To: sgoebel@xbiotech.com Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Easy option is to melt down, and run cassettes through the clean cycle on your processors to remove all the paraffin. then process as usual. I've done this before with decent results. Good luck! Catherine On Wed, Jul 7, 2010 at 11:36 AM, wrote: > > Hello all!! Hope everyone had a happy 4th!! Question today is...I > grossed in some fat that I need to routine process. I ha ve done > this in the past with extra fixation and no problem? This tim e > however it didn't fix all the way through and I have oily unfixed fat > in the middle of my block. I fixed for 48 hours, but guess my > section we re too big and needed more. I want to fix for a little > longer and rep rocess the block. I know I need to melt it down, but > then what? I have done this before, it's just my brain is getting > older =) > > Thanks in advance!! > > Sarah Goebel, B.A., HT ( ASCP) > > Histotechnician > > XBiotech USA Inc. > > > 8201 East Riverside Dr. Bldg 4 Suite 100 > > Austin, Texas 78744 > > (512)386-5107 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 22 Date: Wed, 7 Jul 2010 10:53:18 -0500 From: Cheri Miller Subject: RE: [Histonet] Reprocess To: "sgoebel@xbiotech.com" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run them through the cleaning cycle on my processor. The Xylene and Alcohol will melt the oily fat. When the clean cycle is complete I just drop them back into formalin until I process that evening. It works very well. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 23 Date: Wed, 7 Jul 2010 11:01:37 -0500 From: Cheri Miller Subject: RE: [Histonet] Reprocess To: Cheri Miller , "sgoebel@xbiotech.com" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I take that back I do melt them, recap the cassettes and then drop them in to clean. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, July 07, 2010 10:53 AM To: sgoebel@xbiotech.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocess Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run them through the cleaning cycle on my processor. The Xylene and Alcohol will melt the oily fat. When the clean cycle is complete I just drop them back into formalin until I process that evening. It works very well. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 24 Date: Wed, 7 Jul 2010 11:24:51 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] Reprocess To: "'sgoebel@xbiotech.com'" , "histonet@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2F0@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="utf-8" Cheri's process is a good one, we use it too. Works every time. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. 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Thank you for your cooperation. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 7 *************************************** From brett_connolly <@t> merck.com Wed Jul 7 11:54:20 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Jul 7 11:54:34 2010 Subject: [Histonet] RE: gout crystals In-Reply-To: References: <01NP77QHBPD48WYDX4@vms.tarleton.edu> Message-ID: <63EA0607835FBA4689CEA9EA8B482692033A5145@usctmx1141.merck.com> See these articles- keep formalin fixation to <12 hrs. Vinod Shidham, Ganesh Shidham (2000) Staining Method to Demonstrate Urate Crystals in Formalin-Fixed, Paraffin-Embedded Tissue Sections. Archives of Pathology & Laboratory Medicine: Vol. 124, No. 5, pp. 774-776. Shidham V, Chivukula M, Basir Z, Shidham G. Mod Pathol. 2001 Aug;14(8):806-10. Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of HOOD MS. GLENDA Sent: Wednesday, July 07, 2010 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: gout crystals It is true that gout crystals are water-soluble, and we are all taught that... but a few years ago there was a published article (JOH??) that showed that many crystals DO survive after water-based fixation. Since you already have the specimen in formalin, at least try processing and see if they can be demonstrated. Couldn't hurt, not nearly as much as the patient would for a re-biopsy! Glenda F. Hood, M.Ed., HT(ASCP) Instructor and Program Director Histotechnology Program Tarleton State University 817-926-1101 x6 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 07, 2010 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Rnase free slides? (Caroline Bass) 2. Gout Crytals (Behnaz Sohrab) 3. RE: Gout Crytals (Weems, Joyce) 4. Re: Gout Crytals (Jennifer MacDonald) 5. RE: Gout Crytals (sgoebel@xbiotech.com) 6. Schmitz, Sandy is out of the office. (Sandy.Schmitz@leica-microsystems.com) 7. RE: Gout Crytals (Joyce Cline) 8. Re: bat wing histology (Mohit Chadha) 9. RE: Gout Crytals (Douglas,Joseph) 10. used histology equipment (dcojita@tampabay.rr.com) 11. Re: used histology equipment (Drew Meyer) 12. Re: Rnase free slides? (Emily Sours) 13. Re: bat wing histology (Amos Brooks) 14. Has anyone used a biotin block in their antibody diluent? (Jennifer Campbell) 15. Biotin block (Jim Reilly) 16. RE: Has anyone used a biotin block in their antibody diluent? (Mauger, Joanne) 17. Re: used histology equipment (Kim.Donadio@bhcpns.org) 18. RE: used histology equipment (Douglas,Joseph) 19. RE: used histology equipment (Douglas,Joseph) 20. Reprocess (sgoebel@xbiotech.com) 21. Re: Reprocess (Catherine Simonson) 22. RE: Reprocess (Cheri Miller) 23. RE: Reprocess (Cheri Miller) 24. RE: Reprocess (Mahoney,Janice A) ---------------------------------------------------------------------- Message: 1 Date: Tue, 06 Jul 2010 15:49:58 -0400 From: Caroline Bass Subject: [Histonet] Rnase free slides? To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello, I'm doing RNA work for the first time. My plan is to take a fresh rat brain, block quickly, freeze by immersing in dry-ice cooled isopentane, storing at -80, collecting tissue sections (thickness will be determined, somewhere between 20 and 300 um), and punching the particular regions I need out of the sections. I will then isolate RNA from the punches for qPCR analysis. Questions: 1) does this sound like a viable plan? 2) and suggestions, what to be careful of? 3) where do I have to be careful of Rnase, should I use disposable blades, cleaned with Rnase away? 4) where can I find Rnase free slides, or should I just make my own. I usually use charged slides. Any and all suggestions will be appreciated. I'm new to this and don't know where I will have problems. Thanks! Caroline ------------------------------ Message: 2 Date: Tue, 06 Jul 2010 13:12:49 -0700 From: "Behnaz Sohrab" Subject: [Histonet] Gout Crytals To: Message-ID: <4C332BD0.4347.0054.1@ah.org> Content-Type: text/plain; charset=US-ASCII Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you ------------------------------ Message: 3 Date: Tue, 6 Jul 2010 16:35:41 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Gout Crytals To: Behnaz Sohrab , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015E968425@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Should skip the formalin!!! They are water soluable... :>( -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Tuesday, July 06, 2010 16:13 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Tue, 6 Jul 2010 13:50:19 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Gout Crytals To: "Behnaz Sohrab" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Uric acid crystals are water soluble. Avoid formalin and fix in absolute alcohol and start the processing of the tissue in absolute alcohol. Jennifer "Behnaz Sohrab" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 01:28 PM To cc Subject [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 06 Jul 2010 13:55:24 -0700 From: sgoebel@xbiotech.com Subject: RE: [Histonet] Gout Crytals To: "Behnaz Sohrab" Cc: histonet@lists.utsouthwestern.edu Message-ID: <20100706135524.9e2d9aa830e8449a2412eb1e4f2f067e.8ce03ce3da.wbe@email04. secureserver.net> Content-Type: text/plain; charset="utf-8" If you have fixed in formalin the gout crystals are all gone!!!!& nbsp; Have to start over with a new sample if possible. The crystals Sarah Goebel, B.A. Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, (512)386-5107 -------- Original Message -------- Subject: [Histonet] Gout Crytals From: "Behnaz Sohrab" <[1]SohrabB1@ah. Date: Tue, July 06, 2010 1:12 pm To: <[2]histonet@lis Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alc Thank you _______________________________________________ Histonet mailing list [3]Histonet@lists.utsou [4]http: References 1. 3D"mailto://SohrabB1@ah.org"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" ------------------------------ Message: 6 Date: Tue, 6 Jul 2010 16:00:56 -0500 From: Sandy.Schmitz@leica-microsystems.com Subject: [Histonet] Schmitz, Sandy is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 07/05/2010 and will not return until 07/07/2010. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 7 Date: Tue, 6 Jul 2010 16:56:52 -0400 From: Joyce Cline Subject: RE: [Histonet] Gout Crytals To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" This works for us. Process normally, we usually fix in 100% alcohol. Cut slide Formula 83 or Xylene 20 seconds Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds Eosin for 20 seconds 100% alcohol 20 seconds 100% alcohol 20 seconds Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds Form 83 or Xylene for 20 seconds coverslip normally Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab [SohrabB1@ah.org] Sent: Tuesday, July 06, 2010 4:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 8 Date: Tue, 6 Jul 2010 17:10:17 -0400 From: Mohit Chadha Subject: Re: [Histonet] bat wing histology To: Amos Brooks Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Thank you everyone for replying, much appreciated. Having also talked to people in my dept, I have a rudimentary protocol ready. Of course, I will have to tweak it to see what works. I will be using anti PGP9.5 antibody for neuronal immunology. I am still not sure how to section the wing. In most likelihood, I will be using freezing sliding microtome. The "swiss roll" method sound good and I will definitely try it. I am thinking that since the wing membrane is thin (~30 um), I will also try to use the whole mount of small pieces. Any other thoughts and advice would be appreciated. Thank you, Mohit Chadha, Univ of Maryland. On Fri, Jul 2, 2010 at 4:14 PM, Amos Brooks wrote: > Hi, > I do hope you are looking at cross sections of the wing and not the > flat. That would be very difficult indeed. For good cross sections I would > try a "Swiss Roll". This is a way of demonstrating a large amount of cross > sectional area in small space. Take the membrane and fix it by submersion in > the fixative of your choice. Prior to processing roll the whole membrane up > then cut the membrane log into sections small enough to fit in a cassette. > You can use foam biopsy pads to support this shape. Embed it and section it > on edge to show a long coiled membrane. The hairs should be able to be > displayed in this way as well. To show a lot of membrane at the same time > you could place multiple rolls in one cassette. This should work well. > > Good Luck, > Amos > > > Message: 21 > Date: Thu, 1 Jul 2010 11:46:17 -0400 > From: Mohit Chadha > Subject: [Histonet] bat wing histology > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello everyone, > > This is my very first post and I am desperately looking for help. I am new > to histology, so any help would be much appreciated. > > I am studying the peripheral sensory innervation of bat wings. As a first > step, I would like to demonstrate the innervation pattern on the different > parts of the wing membrane (a whole mount of the wing?). Second, I would > like to demonstrate the mechanoreceptor make-up of the tiny hairs on the > wing membrane. > > Bat wings are highly elastic, with numerous folds, a thickness of about > 35-45 microns (in the species I study), a network of thin collagen bundles, > and pigmented superficial epidermal layers. > I could provide more information if required. > > Hoping to hear back from the members. > Thank you. > ------------------------------ Message: 9 Date: Tue, 6 Jul 2010 16:22:46 -0500 From: "Douglas,Joseph" Subject: RE: [Histonet] Gout Crytals To: 'Jennifer MacDonald' , Behnaz Sohrab Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" REMOVE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, July 06, 2010 3:50 PM To: Behnaz Sohrab Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Gout Crytals Uric acid crystals are water soluble. Avoid formalin and fix in absolute alcohol and start the processing of the tissue in absolute alcohol. Jennifer "Behnaz Sohrab" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 01:28 PM To cc Subject [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 6 Jul 2010 18:29:09 -0400 From: Subject: [Histonet] used histology equipment To: "'histonet'" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone know of a reputable dealer for used equipment? ------------------------------ Message: 11 Date: Tue, 6 Jul 2010 18:55:53 -0400 From: Drew Meyer <41dmb41@gmail.com> Subject: Re: [Histonet] used histology equipment To: dcojita@tampabay.rr.com Cc: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Absolutely... Southeast Pathology Instrument Service out of Charleston, SC. The owner's name is Michael Dietrich. I've done business with him before and they are great people, very honest and they stand behind their instruments. I would highly recommend them to anyone. Contact Michael directly and tell him Drew Meyer from Atlanta referred you. http://southeastpathology.com/ Drew On Tue, Jul 6, 2010 at 18:29, wrote: > Does anyone know of a reputable dealer for used equipment? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Tue, 6 Jul 2010 19:24:44 -0400 From: Emily Sours Subject: Re: [Histonet] Rnase free slides? To: Caroline Bass , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Honestly, I think RNases are a bunch of hooha. If you're being careful anyway because you're doing a PCR, that should be enough. Wear gloves, be sterile. When I worked with a Russian post-doc, she said she did RNA in situ hybridization without gloves and it worked. Of course, god only knows what her protocol was, as she had some crazy stories about Russian labs. Emily -- Dark Pictures, thrones and stones that pilgrims kiss And poems that take a thousand years to die But ape the immortality of this Red label on a little butterfly. -Vladimir Nabokov, concluding stanza of ???A Discovery??? 1941. On Tue, Jul 6, 2010 at 3:49 PM, Caroline Bass wrote: > Hello, > > I'm doing RNA work for the first time. My plan is to take a fresh rat brain, > block quickly, freeze by immersing in dry-ice cooled isopentane, storing at > -80, collecting tissue sections (thickness will be determined, somewhere > between 20 and 300 um), and punching the particular regions I need out of > the sections. I will then isolate RNA from the punches for qPCR analysis. > > Questions: > > 1) does this sound like a viable plan? > 2) and suggestions, what to be careful of? > 3) where do I have to be careful of Rnase, should I use disposable blades, > cleaned with Rnase away? > 4) where can I find Rnase free slides, or should I just make my own. I > usually use charged slides. > > Any and all suggestions will be appreciated. I'm new to this and don't know > where I will have problems. > > Thanks! > > Caroline > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Tue, 6 Jul 2010 22:10:44 -0400 From: Amos Brooks Subject: Re: [Histonet] bat wing histology To: Mohit Chadha Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, We did this on derm punch biopsies for a while. We used PLP (paraformaldehyde-lysine periodate) fixative. This was also done on a sliding microtome at 50um with piles of dry ice to keep the sucrose frozen. It was a bit of a pain in the butt, but we got decent results. I think the sectioning process could have been easier, but the PI was obsessed with not modifying the project at all, even if it meant improvements. We stained these as floating sections in 96 well plates, so in this case I would be concerned that the wing rolls would un-roll if you were to do the swiss roll thing I described. Have fun :-) Amos On Tue, Jul 6, 2010 at 5:10 PM, Mohit Chadha wrote: > Thank you everyone for replying, much appreciated. > > Having also talked to people in my dept, I have a rudimentary protocol > ready. Of course, I will have to tweak it to see what works. I will be using > anti PGP9.5 antibody for neuronal immunology. > > I am still not sure how to section the wing. In most likelihood, I will be > using freezing sliding microtome. The "swiss roll" method sound good and I > will definitely try it. I am thinking that since the wing membrane is thin > (~30 um), I will also try to use the whole mount of small pieces. > > Any other thoughts and advice would be appreciated. > > Thank you, > Mohit Chadha, > Univ of Maryland. > > > > > > > > On Fri, Jul 2, 2010 at 4:14 PM, Amos Brooks wrote: > >> Hi, >> I do hope you are looking at cross sections of the wing and not the >> flat. That would be very difficult indeed. For good cross sections I would >> try a "Swiss Roll". This is a way of demonstrating a large amount of cross >> sectional area in small space. Take the membrane and fix it by submersion in >> the fixative of your choice. Prior to processing roll the whole membrane up >> then cut the membrane log into sections small enough to fit in a cassette. >> You can use foam biopsy pads to support this shape. Embed it and section it >> on edge to show a long coiled membrane. The hairs should be able to be >> displayed in this way as well. To show a lot of membrane at the same time >> you could place multiple rolls in one cassette. This should work well. >> >> Good Luck, >> Amos >> >> >> Message: 21 >> Date: Thu, 1 Jul 2010 11:46:17 -0400 >> From: Mohit Chadha >> Subject: [Histonet] bat wing histology >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hello everyone, >> >> This is my very first post and I am desperately looking for help. I am new >> to histology, so any help would be much appreciated. >> >> I am studying the peripheral sensory innervation of bat wings. As a first >> step, I would like to demonstrate the innervation pattern on the different >> parts of the wing membrane (a whole mount of the wing?). Second, I would >> like to demonstrate the mechanoreceptor make-up of the tiny hairs on the >> wing membrane. >> >> Bat wings are highly elastic, with numerous folds, a thickness of about >> 35-45 microns (in the species I study), a network of thin collagen >> bundles, >> and pigmented superficial epidermal layers. >> I could provide more information if required. >> >> Hoping to hear back from the members. >> Thank you. >> > > ------------------------------ Message: 14 Date: Tue, 6 Jul 2010 19:11:14 -0700 From: "Jennifer Campbell" Subject: [Histonet] Has anyone used a biotin block in their antibody diluent? To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FE1@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="iso-8859-1" Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell ------------------------------ Message: 15 Date: Wed, 7 Jul 2010 09:05:17 +0100 From: "Jim Reilly" Subject: [Histonet] Biotin block To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Jennifer I use the Avidin/Biotin blocking kit from Vector SP-2001 I mix the Avidin D with my normal blocking serum and the biotin I add to my primary antibody diluent. This seems to work well for most tissue types. Cheers Jim ------------------------------ Message: 16 Date: Wed, 7 Jul 2010 07:32:52 -0400 From: "Mauger, Joanne" Subject: [Histonet] RE: Has anyone used a biotin block in their antibody diluent? To: Jennifer Campbell , "histonet@lists.utsouthwestern.edu" Message-ID: <443F5B475A9BF647AB962E834884EBAD278D1277FE@EX7CCRPW03V1.chop.edu> Content-Type: text/plain; charset="us-ascii" Jennifer, Vector has an avidin-biotin blocking kit that works well by itself or mixed with reagents- very reasonable cost. Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell [jcampbell@vdxpathology.com] Sent: Tuesday, July 06, 2010 10:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Has anyone used a biotin block in their antibody diluent? Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 7 Jul 2010 08:37:27 -0500 From: Kim.Donadio@bhcpns.org Subject: Re: [Histonet] used histology equipment To: Cc: 'histonet' , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi Diane, Because of your location I would recommend Micro-optics of Florida. Mike Jones or Tom Christy. The number is 954-791-0082. They are great people to work with and I have found them very reasonable on their prices. Hope this helps! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 05:29 PM To "'histonet'" cc Subject [Histonet] used histology equipment Does anyone know of a reputable dealer for used equipment? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 18 Date: Wed, 7 Jul 2010 08:40:37 -0500 From: "Douglas,Joseph" Subject: RE: [Histonet] used histology equipment To: 'Drew Meyer' <41dmb41@gmail.com>, "dcojita@tampabay.rr.com" Cc: histonet Message-ID: Content-Type: text/plain; charset="us-ascii" REMOVE FROM DATABASE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, July 06, 2010 5:56 PM To: dcojita@tampabay.rr.com Cc: histonet Subject: Re: [Histonet] used histology equipment Absolutely... Southeast Pathology Instrument Service out of Charleston, SC. The owner's name is Michael Dietrich. I've done business with him before and they are great people, very honest and they stand behind their instruments. I would highly recommend them to anyone. Contact Michael directly and tell him Drew Meyer from Atlanta referred you. http://southeastpathology.com/ Drew On Tue, Jul 6, 2010 at 18:29, wrote: > Does anyone know of a reputable dealer for used equipment? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 7 Jul 2010 08:41:59 -0500 From: "Douglas,Joseph" Subject: RE: [Histonet] used histology equipment To: "'Kim.Donadio@bhcpns.org'" , "dcojita@tampabay.rr.com" Cc: 'histonet' , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" REMOVE FROM DATABASE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, July 07, 2010 8:37 AM To: dcojita@tampabay.rr.com Cc: 'histonet'; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] used histology equipment Hi Diane, Because of your location I would recommend Micro-optics of Florida. Mike Jones or Tom Christy. The number is 954-791-0082. They are great people to work with and I have found them very reasonable on their prices. Hope this helps! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Sent by: histonet-bounces@lists.utsouthwestern.edu 07/06/2010 05:29 PM To "'histonet'" cc Subject [Histonet] used histology equipment Does anyone know of a reputable dealer for used equipment? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 07 Jul 2010 08:36:47 -0700 From: sgoebel@xbiotech.com Subject: [Histonet] Reprocess To: histonet@lists.utsouthwestern.edu Message-ID: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04. secureserver.net> Content-Type: text/plain; charset="utf-8" Hello all!! Hope everyone had a happy 4th!! Question grossed in some fat that I need to routine process. I ha this in the past with extra fixation and no problem? This tim however it didn't fix all the way through and I have oily unfixed fat in section we longer and rep then what? older =) Thanks in advance!! Sarah Goebel, B.A., HT ( Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 ------------------------------ Message: 21 Date: Wed, 7 Jul 2010 11:47:39 -0400 From: Catherine Simonson Subject: Re: [Histonet] Reprocess To: sgoebel@xbiotech.com Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Easy option is to melt down, and run cassettes through the clean cycle on your processors to remove all the paraffin. then process as usual. I've done this before with decent results. Good luck! Catherine On Wed, Jul 7, 2010 at 11:36 AM, wrote: > > Hello all!! Hope everyone had a happy 4th!! Question today is...I > grossed in some fat that I need to routine process. I ha ve done > this in the past with extra fixation and no problem? This tim e > however it didn't fix all the way through and I have oily unfixed fat > in the middle of my block. I fixed for 48 hours, but guess my > section we re too big and needed more. I want to fix for a little > longer and rep rocess the block. I know I need to melt it down, but > then what? I have done this before, it's just my brain is getting > older =) > > Thanks in advance!! > > Sarah Goebel, B.A., HT ( ASCP) > > Histotechnician > > XBiotech USA Inc. > > > 8201 East Riverside Dr. Bldg 4 Suite 100 > > Austin, Texas 78744 > > (512)386-5107 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 22 Date: Wed, 7 Jul 2010 10:53:18 -0500 From: Cheri Miller Subject: RE: [Histonet] Reprocess To: "sgoebel@xbiotech.com" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run them through the cleaning cycle on my processor. The Xylene and Alcohol will melt the oily fat. When the clean cycle is complete I just drop them back into formalin until I process that evening. It works very well. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 23 Date: Wed, 7 Jul 2010 11:01:37 -0500 From: Cheri Miller Subject: RE: [Histonet] Reprocess To: Cheri Miller , "sgoebel@xbiotech.com" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I take that back I do melt them, recap the cassettes and then drop them in to clean. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, July 07, 2010 10:53 AM To: sgoebel@xbiotech.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocess Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run them through the cleaning cycle on my processor. The Xylene and Alcohol will melt the oily fat. When the clean cycle is complete I just drop them back into formalin until I process that evening. It works very well. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 24 Date: Wed, 7 Jul 2010 11:24:51 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] Reprocess To: "'sgoebel@xbiotech.com'" , "histonet@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2F0@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="utf-8" Cheri's process is a good one, we use it too. Works every time. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. 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Thank you for your cooperation. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 7 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From mfredrickson <@t> nrh-ok.com Wed Jul 7 12:03:55 2010 From: mfredrickson <@t> nrh-ok.com (Fredrickson, Mona) Date: Wed Jul 7 12:04:00 2010 Subject: [Histonet] (no subject) Message-ID: Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma

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From Jackie.O'Connor <@t> abbott.com Wed Jul 7 12:09:59 2010 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jul 7 12:10:33 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: Ask management if he would suddenly be a better technician and more valuable if he passed the exam? Duh - no. In my opinion, merit increases should be based on merit - not documentation. If he's not eligible, he's not eligible. Does your management increase pay based on certification? So, if a certified tech with one year of experience came to your lab, they would be paid more than an incumbent tech with 8 years experience? Sounds like you have some dinosaur management there. From: "Fredrickson, Mona" To: "Histonet@lists.utsouthwestern.edu" Date: 07/07/2010 12:04 PM Subject: [Histonet] (no subject) Sent by: histonet-bounces@lists.utsouthwestern.edu Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma

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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgill <@t> marylandgeneral.org Wed Jul 7 12:19:06 2010 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Wed Jul 7 12:19:15 2010 Subject: [Histonet] QC log In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFC8E@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFC8E@LBEXCH01.hchd.local> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9EAF@MDGEN-EXCH1.marylandgeneral.org> This is the QC sheet we use and the Pathologist documents any issues there may be if any. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, July 07, 2010 12:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QC log Hello to all in histoland. Does anyone have a QC log sheet that documents the H&E stain, microtomy, floaters and other issues that they would be willing to share with me. Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Jul 7 12:21:02 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Jul 7 12:21:08 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5B1FB29@LRGHEXVS1.practice.lrgh.org> That is a hard question. Here when we hire a un registered tech, they are given a time limit (3 years) to get their certification. At the end of three years, we reserve the right to terminate if they have not achieved their certification. What are the levels for your lab? Do you have anything like Lab Scientist 1, 2 or 3 and if so are there pay ranges between each level? If there are levels like that, I as manager, would keep him in level 1 with no salary increase until the degree is finished. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fredrickson, Mona [mfredrickson@nrh-ok.com] Sent: Wednesday, July 07, 2010 1:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma CONFIDENTIALITY NOTICE:

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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From cgill <@t> marylandgeneral.org Wed Jul 7 12:28:28 2010 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Wed Jul 7 12:28:31 2010 Subject: [Histonet] QC log In-Reply-To: <087A9911BBAFDE4B8151CB148586E2C23A9EAF@MDGEN-EXCH1.marylandgeneral.org> References: <1872B4A455B7974391609AD8034C79FC026DFC8E@LBEXCH01.hchd.local> <087A9911BBAFDE4B8151CB148586E2C23A9EAF@MDGEN-EXCH1.marylandgeneral.org> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9EB0@MDGEN-EXCH1.marylandgeneral.org> I forgot to say that we use a different one for specials so if you need that also let me know. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gill, Caula A. Sent: Wednesday, July 07, 2010 1:19 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] QC log This is the QC sheet we use and the Pathologist documents any issues there may be if any. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, July 07, 2010 12:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QC log Hello to all in histoland. Does anyone have a QC log sheet that documents the H&E stain, microtomy, floaters and other issues that they would be willing to share with me. Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgunder1 <@t> Fairview.org Wed Jul 7 12:31:44 2010 From: mgunder1 <@t> Fairview.org (Gunderson, Michael) Date: Wed Jul 7 12:32:03 2010 Subject: [Histonet] (no subject) In-Reply-To: References: , Message-ID: <45EBFA1E7C931E45BBA7172D42F23C9205E83376F7@EXCH-MBX5.Fairview.org> By telling someone they should earn the same without passing our registration exam diminishes the effort and hard work of registered HT and HTL's. Management are not dinosaurs, they are fair. The exam is there to weed out the weak and under or un-qualified, if you cannot pass the test, you have not earned the right of those who have. Michael A. Gunderson HTL(ASCP) Lead Technologist-Immunostains Laboratory University of Minnesota Medical Center-Fairview 2450 Riverside Avenue Minneapolis, MN 55454 ________________________________________ Laboratory: 1-612-273-9119 Fax: 1-612-273-4879 Email: mgunder1@fairview.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor [Jackie.O'Connor@abbott.com] Sent: Wednesday, July 07, 2010 12:09 PM To: Fredrickson, Mona Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Ask management if he would suddenly be a better technician and more valuable if he passed the exam? Duh - no. In my opinion, merit increases should be based on merit - not documentation. If he's not eligible, he's not eligible. Does your management increase pay based on certification? So, if a certified tech with one year of experience came to your lab, they would be paid more than an incumbent tech with 8 years experience? Sounds like you have some dinosaur management there. From: "Fredrickson, Mona" To: "Histonet@lists.utsouthwestern.edu" Date: 07/07/2010 12:04 PM Subject: [Histonet] (no subject) Sent by: histonet-bounces@lists.utsouthwestern.edu Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma

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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed Jul 7 12:35:19 2010 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jul 7 12:35:52 2010 Subject: [Histonet] (no subject) In-Reply-To: <45EBFA1E7C931E45BBA7172D42F23C9205E83376F7@EXCH-MBX5.Fairview.org> References: , <45EBFA1E7C931E45BBA7172D42F23C9205E83376F7@EXCH-MBX5.Fairview.org> Message-ID: Over the years, I have had some HT and HTL registered technologists who were crummy technicians. I'm jus' sayin' that certification doesn't automatically mean you are better than a non-certified tech. From: "Gunderson, Michael" To: Jackie M O'Connor , "Fredrickson, Mona" Cc: "Histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Date: 07/07/2010 12:31 PM Subject: RE: [Histonet] (no subject) By telling someone they should earn the same without passing our registration exam diminishes the effort and hard work of registered HT and HTL's. Management are not dinosaurs, they are fair. The exam is there to weed out the weak and under or un-qualified, if you cannot pass the test, you have not earned the right of those who have. Michael A. Gunderson HTL(ASCP) Lead Technologist-Immunostains Laboratory University of Minnesota Medical Center-Fairview 2450 Riverside Avenue Minneapolis, MN 55454 ________________________________________ Laboratory: 1-612-273-9119 Fax: 1-612-273-4879 Email: mgunder1@fairview.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor [Jackie.O'Connor@abbott.com] Sent: Wednesday, July 07, 2010 12:09 PM To: Fredrickson, Mona Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Ask management if he would suddenly be a better technician and more valuable if he passed the exam? Duh - no. In my opinion, merit increases should be based on merit - not documentation. If he's not eligible, he's not eligible. Does your management increase pay based on certification? So, if a certified tech with one year of experience came to your lab, they would be paid more than an incumbent tech with 8 years experience? Sounds like you have some dinosaur management there. From: "Fredrickson, Mona" To: "Histonet@lists.utsouthwestern.edu" Date: 07/07/2010 12:04 PM Subject: [Histonet] (no subject) Sent by: histonet-bounces@lists.utsouthwestern.edu Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma

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This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments.

_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Wed Jul 7 12:38:03 2010 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Wed Jul 7 12:38:07 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <275948.59020.qm@web57808.mail.re3.yahoo.com> I agree with Tom and Michael.?Those who have been fortunate enough to pass deserve the kudos and pay increase.?As for the title, he simply isn't eligible at this point. He is a non-registered tech. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ________________________________ From: "Fredrickson, Mona" To: "Histonet@lists.utsouthwestern.edu" Sent: Wed, July 7, 2010 1:03:55 PM Subject: [Histonet] (no subject) Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science.? The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase.? But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma

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This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments.

_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Jul 7 12:40:24 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jul 7 12:41:05 2010 Subject: [Histonet] (no subject) In-Reply-To: References: , <45EBFA1E7C931E45BBA7172D42F23C9205E83376F7@EXCH-MBX5.Fairview.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EAF72C5D9@IBMB7Exchange.digestivespecialists.com> I have known some really crummy MD's too but I still wouldn't want to go to a physician that couldn't pass his boards. I sympathize with the person that didn't pass the exam and that doesn't make him a poor histotech just not one that gets the HT (ASCP) after his name and not eligible for the same pay scale as that of a registered tech. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, July 07, 2010 1:35 PM To: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Fredrickson, Mona Subject: RE: [Histonet] (no subject) Over the years, I have had some HT and HTL registered technologists who were crummy technicians. I'm jus' sayin' that certification doesn't automatically mean you are better than a non-certified tech. From: "Gunderson, Michael" To: Jackie M O'Connor , "Fredrickson, Mona" Cc: "Histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Date: 07/07/2010 12:31 PM Subject: RE: [Histonet] (no subject) By telling someone they should earn the same without passing our registration exam diminishes the effort and hard work of registered HT and HTL's. Management are not dinosaurs, they are fair. The exam is there to weed out the weak and under or un-qualified, if you cannot pass the test, you have not earned the right of those who have. Michael A. Gunderson HTL(ASCP) Lead Technologist-Immunostains Laboratory University of Minnesota Medical Center-Fairview 2450 Riverside Avenue Minneapolis, MN 55454 ________________________________________ Laboratory: 1-612-273-9119 Fax: 1-612-273-4879 Email: mgunder1@fairview.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor [Jackie.O'Connor@abbott.com] Sent: Wednesday, July 07, 2010 12:09 PM To: Fredrickson, Mona Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Ask management if he would suddenly be a better technician and more valuable if he passed the exam? Duh - no. In my opinion, merit increases should be based on merit - not documentation. If he's not eligible, he's not eligible. Does your management increase pay based on certification? So, if a certified tech with one year of experience came to your lab, they would be paid more than an incumbent tech with 8 years experience? Sounds like you have some dinosaur management there. From: "Fredrickson, Mona" To: "Histonet@lists.utsouthwestern.edu" Date: 07/07/2010 12:04 PM Subject: [Histonet] (no subject) Sent by: histonet-bounces@lists.utsouthwestern.edu Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma

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This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments.

_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Wed Jul 7 12:53:12 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Jul 7 12:53:25 2010 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: It seems to me that Histotech non-registered without pay increase would be appropriate. Why would you change his title and give him an increase in pay when he didn't fulfill his employment agreement? I would not decrease his pay and I would not terminate a good employee. Apparently he earned his present pay as an "undocumented" non-registered Histotech and nothing has changed. He should continue to get merit increases as per usual, with the goal of getting the Associates Degree, and registry (if still interested). He should be allowed to perform any function he has been deemed competent to perform. Just my 2 cents. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Fredrickson, Mona" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/07/2010 01:04 PM To "Histonet@lists.utsouthwestern.edu" cc Subject [Histonet] (no subject) Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma

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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From cmiller <@t> physlab.com Wed Jul 7 13:21:52 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jul 7 13:21:58 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: He did not meet his and his employers agreed stipulations for employment. If he was being paid equal to that of a registered tech then he should receive a decrease in pay. If not then he should continue with merit increases only. Getting an ASCP registry is a marketable achievement for both the tech and the employer. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From sgoebel <@t> xbiotech.com Wed Jul 7 13:35:13 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Jul 7 13:35:18 2010 Subject: [Histonet] (no subject) Message-ID: <20100707113513.9e2d9aa830e8449a2412eb1e4f2f067e.6091f08455.wbe@email04.secureserver.net> I totally agree!!! Why did I spend the time, effort, and mo (additionally to stay certified) if I am not going to get a pay incenti would you as someone who had exam...of course not (in fact y person!). I have worked in places "non-registered histotechs", my question always was.. this position? I had to pay my dues as an underpaid lab like I'm sure everyone else did while getting my degree and regi stry...why shouldn't everyone else? I know in the past it really gets an hour th say listen to money you make!!! Sarah G Histotechnician XBiote 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] (no subject) From: "Gunderson, Michael" <[1]m Date: Wed, July 07, 2010 10:31 am To: Jackie M O'Connor Cc: "[4]Histonet@lists. <[5]Histonet@lists.u "[6]histonet-bo <[7]histonet By telling someone they should earn the same without passing our registrati HT and HTL's. Ma is there to weed out cannot pass the test, you have n have. Michael A. Gunderson HTL(ASCP) Lead Technologist-Immunostains Laboratory University of Minnesota Medical Center-Fairview 2450 Riverside Avenue Minneapolis, MN 55454 ________________________________________ Laboratory: 1-612-273-9119 Fax: 1-612-273-4879 Email: [8]mgunder1@fairview.org ________________________________________ From: [9]histon [[10]histonet-bounces@lists.utsouthwestern.edu] O'Connor [Jackie.O'[11]Connor@abbott.com] Sent: Wednesday, July 07, 2010 12:09 PM To: Fredrickson, Mona Cc: [12]Histonet@lists.u [13]histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Ask management if he would suddenly be a better technician and more valuable if he passed the exam? Duh - no. In my opinion, merit increases should be based on merit - not documentation. If he's not eligible, he's not eligible. Does your management increase pay based on certification? So, if a certified tech with one year of experience came to your lab, they would be paid more than an incumbent tech with 8 years experience? Sounds like you have some dinosaur management there. From: "Fredrickson, Mona" <[14]mfredr To: "[15]Histonet@lists.utso <[16]Histonet@lists.utsouthwestern.edu> Date: 07/07/2010 12:04 PM Subject: [Histonet] (no subject) Sent by: [17]histonet-bou Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma

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_______________________________________________ Histonet mailing list [18]Histonet@lists.utsou [19]http: _______________________________________________ Histonet mailing list [20]Histonet@lists.utsou [21]http: _______________________________________________ Histonet mailing list [22]Histonet@lists.utsou [23]http: References 1. 3D"mailto://mgunder1@Fairview.org"/ 2. 3D"mailto://Connor@abbott.com"/ 3. 3D"mailto://mfredrickson@nrh-ok.com"/ 4. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 5. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 6. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 7. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 8. 3D"mailto://mgunder1@fairview.org"/ 9. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 10. 3D"mailto://histonet-bounc=/ 11. 3D"mailto://Connor@abbott=/ 12. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 13. 3D"mailto://histonet-bounces@lists.utsouthwe=/ 14. 3D"mailto://mfredrickson@nrh-ok.com"/ 15. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 16. 3D"mailto://Histonet@lists.utsouthwestern.e=/ 17. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 18. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 19. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 20. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 21. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 22. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 23. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From sgoebel <@t> xbiotech.com Wed Jul 7 13:39:10 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Jul 7 13:39:14 2010 Subject: [Histonet] Reprocess Message-ID: <20100707113910.9e2d9aa830e8449a2412eb1e4f2f067e.8f4b21c8b6.wbe@email04.secureserver.net> Is this run backwards ok? I want to keep the fat. In want the fat? Sarah Goebel, B.A., HT (ASCP) Histot XBiotech USA Inc. 8201 East Riversi Austin, Texas 78744< (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] Reprocess From: Cheri Miller <[1]cmiller@phy Date: Wed, July 07, 2010 8:53 am To: "[2]sgoebel@xbiotech.com" &l "[4]histonet@lists.utso <[5]histonet@lists.utsouthwestern.edu> Hi Sarah, I don't melt them down I just drop them in with my dirty molds an processor. The Xylene clean cycle is complete I just process that evening. It works very w Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: [6]histon [[7]mailto:histonet-bounces@lists.utsouthwestern.edu [8]sgoebel@xbiote Sent: Wednesday, July 07, 2010 10:37 AM To: [9]histonet@lists.u Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list [10]Histonet@lists.utsou [11]http: PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If responsible for de notified that you are in posse information. Any dissemination, distr e-mail is strictly prohibited. If you have rec error, please notify the sender immediately and delet from your system. References 1. 3D"mailto://cmiller@physlab.com"/ 2. 3D"mailto://sgoebel@xbiotech.com"/ 3. 3D"mailto://sgoebel@xbiotech.com"/ 4. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 5. 3D"mailto://histonet@lists.utsouthwestern.e=/ 6. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 7. 3D"mailto:histonet-bounces 8. 3D"mailto://sgoebel@xbiotech.com"/ 9. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 10. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From carl.hobbs <@t> kcl.ac.uk Wed Jul 7 14:31:07 2010 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Jul 7 14:31:41 2010 Subject: [Histonet] Re: Has anyone used a biotin block in their antibody diluent? Message-ID: <11D9615B89C10747B1C985966A63D7CA2D7BAE9928@KCL-MAIL04.kclad.ds.kcl.ac.uk> I assume that you are using a biotinylated detection system? If so, endogenous biotin labelling is specific, tho' NOT wanted ;-) So, before you try variations, just buy a kit and see if your "non-specific" immunostaining is due to endog. biotin ...follow their instructions by pre-incubating in the kit. Then, if you find that the staining you get is due to endog. biotin...you can modify away ( such as including in primary Ab: However, the kits are RTU: so, by adding to primary Ab, for eg, you use a heck of a lot of kit solution.....;-) I always pre-incubate because there are so many tissues that do NOT require endog. biotin blocking. Also, if you discover that endogenous biotin is the problem and you will therefore need to use a biotin blocking kit regularly, why not make up your own? Far cheaper/equally effective: many sites give protocols, such as this one http://www.immunoportal.com/ Please Post further if you have any problems. Good luck. From Keri.Colwell <@t> inspection.gc.ca Wed Jul 7 16:10:20 2010 From: Keri.Colwell <@t> inspection.gc.ca (Keri Colwell) Date: Wed Jul 7 16:10:28 2010 Subject: [Histonet] DAB Message-ID: <4C3498DC.C0AD.00BF.0@inspection.gc.ca> Hello Histonetters, I am currently trying to work out a new DAB protocol to be used with IHC on FFPE sections. I am testing three different protocols, and am having limited success. The first protocol requires me to make a DAB stock solution of 10mg in 1 ml dH20, which is then filtered and frozen immediately. This protocol suggests using DAB tetrahydrochloride XH20, but I am using the free base of DAB as that is what was on hand. The free base does not want to go into solution, but has produced some reaction in my IHC. My second protocol asks for 40mg DAB in 1.5 ml Tris buffer (0.05M, pH 7.6), to make my stock solution. I am again using the free base, and have been stirring since 11:30 am yesterday (MDT) with the result being a chocolate milk-like substance. My third protocol requires me to make up the DAB fresh each time I want to use it. This protocol also asks for the tetrahydrochloride form, but again I used the free base and did get some reaction. Does anyone have thoughts on about how to get the free base to go into solution without oxidizing, or thoughts about free base versus the other form of DAB? Thanks! Keri Keri Colwell Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell@inspection.gc.ca Telephone | T?l?phone: 403-382-5500 Facsimile | T?l?copieur: 403-382-5583 Government of Canada | Gouvernement du Canada From amosbrooks <@t> gmail.com Wed Jul 7 16:13:06 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Jul 7 16:13:12 2010 Subject: [Histonet] Rnase free slides? Message-ID: Hi, This is certainly possible, but RNAase is everywhere, and in a cryostat you really can't remove it because the RNA remover is an aqueous solution that will freeze in the cryostat. A disposable blade (RNAase cleaned) is probably the best you can do there. One thing to consider, I'm sure OCT isn't RNAase free, so perhaps you should use 30% in DEPC treated water. I have not seen RNAase free slides being sold by any vendors. I usually use LCM slides, but your are doing this a bit differently. For this I wouldn't waste my money on buying RNAase free slides. You can just dip the slides in RNAase away and let them air dry (use them soon after though). Further, I would not use charged slides as that would be counter-productive. You are trying to get tissues off the slides, so adhesives would not be helpful to you. Just a plain ole' glass slide with no bells or whistles. Come to think of it that may be hard to find too ;-) Good luck, Amos Message: 1 Date: Tue, 06 Jul 2010 15:49:58 -0400 From: Caroline Bass Subject: [Histonet] Rnase free slides? To: Message-ID: > Content-Type: text/plain; charset="US-ASCII" Hello, I'm doing RNA work for the first time. My plan is to take a fresh rat brain, block quickly, freeze by immersing in dry-ice cooled isopentane, storing at -80, collecting tissue sections (thickness will be determined, somewhere between 20 and 300 um), and punching the particular regions I need out of the sections. I will then isolate RNA from the punches for qPCR analysis. Questions: 1) does this sound like a viable plan? 2) and suggestions, what to be careful of? 3) where do I have to be careful of Rnase, should I use disposable blades, cleaned with Rnase away? 4) where can I find Rnase free slides, or should I just make my own. I usually use charged slides. Any and all suggestions will be appreciated. I'm new to this and don't know where I will have problems. Thanks! Caroline From histotech90 <@t> cox.net Wed Jul 7 17:59:56 2010 From: histotech90 <@t> cox.net (histotech90@cox.net) Date: Wed Jul 7 17:59:58 2010 Subject: [Histonet] Looking for Histology position in the Seattle Washington area Message-ID: <20100707185956.VPUIG.916545.imail@fed1rmwml38> Hello Netters! I am trying to relocate to the Seattle Washington area and looking for a Histology position. I have 17 years experience, have set up labs as consultant and also have 5 years managememt experience. Will consider any position. I am also HT ascp. Thank you in advance!! From jclark <@t> pcnm.com Wed Jul 7 18:00:54 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Jul 7 18:00:58 2010 Subject: [Histonet] universal microtome aligner Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C012D7C2C@mail.pcnm.com> We purchased this item from Newcomer supply and have been universally disappointed in it. The concept for the tool is good, but we have found that it will not clamp tightly onto our Leica microtomes (model RM2125). Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico From rsrichmond <@t> gmail.com Wed Jul 7 18:31:16 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jul 7 18:31:19 2010 Subject: [Histonet] Re: gout crystals Message-ID: Gout crystals are monosodium urate. In clinical material they're found in large masses, called tophi (TOE-fie, singular tophus). The individual crystals are long and needle-like, with negative birefringence if your pathologist is so fortunate as to have a full wave plate (a.k.a. first order plate, gout slider) on their microscope. The alcohol fixation and processing routine is ideal, but rarely achievable. The specimen will normally come to you in formalin. The crystal masses in the tophi can be picked out with the point of a scalpel blade (if you're still allowed scalpel blades with points on them) into water or alcohol, and looked at as a wet preparation in a polarizing microscope, where you can see the crystals with the aid of a polarizer. Calcium pyrophosphate ("pseudogout") crystals, blocky with positive birefringence, can also be identified with this technique. The CPT code for crystal examination cannot, I think, be assigned in addition to the 88305. Bob Richmond Samurai Pathologist Knoxville TN From talulahgosh <@t> gmail.com Wed Jul 7 18:32:10 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Jul 7 18:32:21 2010 Subject: [Histonet] Rnase free slides? In-Reply-To: References: Message-ID: Am I wrong in assuming that the glass slides I get (which are in a plastic box, wrapped in plastic) are not RNase free? They're superfrost plus from Fisher. It hasn't been a problem for us to assume so, but I'm wondering what other labs think about it. We've never treated our slides with Rnase Away or rinsed in DEPC water. We use DEPC water for in situ hybridization solutions. The in situ dishes are sprayed with RNase away, then DEPC water. Also use OCT when you section, it'll make your life so much easier! It's assumed in my lab that if a chemical came in and hasn't been opened, it's RNase free. To keep it RNase free, you always wear gloves. Store your slides in a new box to keep them RNase free. RNases are everywhere on people, I assumed, and that was the problem. I didn't think RNases are everywhere in the universe on every object. But I could be wrong. Anyone else have an opinion? Emily -- Dark Pictures, thrones and stones that pilgrims kiss And poems that take a thousand years to die But ape the immortality of this Red label on a little butterfly. -Vladimir Nabokov, concluding stanza of ?A Discovery? 1941. On Wed, Jul 7, 2010 at 5:13 PM, Amos Brooks wrote: > Hi, > ? ? This is certainly possible, but RNAase is everywhere, and in a cryostat > you really can't remove it because the RNA remover is an aqueous solution > that will freeze in the cryostat. A disposable blade (RNAase cleaned) is > probably the best you can do there. One thing to consider, I'm sure OCT > isn't RNAase free, so perhaps you should use 30% in DEPC treated water. > ? ? I have not seen RNAase free slides being sold by any vendors. I usually > use LCM slides, but your are doing this a bit differently. For this I > wouldn't waste my money on buying RNAase free slides. You can just dip the > slides in RNAase away and let them air dry (use them soon after though). > Further, I would not use charged slides as that would be counter-productive. > You are trying to get tissues off the slides, so adhesives would not be > helpful to you. Just a plain ole' glass slide with no bells or whistles. > Come to think of it that may be hard to find too ;-) > > Good luck, > Amos > > > Message: 1 > Date: Tue, 06 Jul 2010 15:49:58 -0400 > From: Caroline Bass > Subject: [Histonet] Rnase free slides? > To: > Message-ID: >> > Content-Type: text/plain; ? ? ? charset="US-ASCII" > > Hello, > > I'm doing RNA work for the first time. My plan is to take a fresh rat brain, > block quickly, freeze by immersing in dry-ice cooled isopentane, storing at > -80, collecting tissue sections (thickness will be determined, somewhere > between 20 and 300 um), and punching the particular regions I need out of > the sections. I will then isolate RNA from the punches for qPCR analysis. > > Questions: > > 1) does this sound like a viable plan? > 2) and suggestions, what to be careful of? > 3) where do I have to be careful of Rnase, should I use disposable blades, > cleaned with Rnase away? > 4) where can I find Rnase free slides, or should I just make my own. I > usually use charged slides. > > Any and all suggestions will be appreciated. I'm new to this and don't know > where I will have problems. > > Thanks! > > Caroline > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AnthonyH <@t> chw.edu.au Wed Jul 7 18:48:14 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jul 7 18:48:28 2010 Subject: [Histonet] Reprocess In-Reply-To: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> Message-ID: You do not need to rehydrate the blocks back to water, While the tissue is still hot (ie the wax is still molten) blot the tissue dry, place it back in its cassette and place the cassette in 10% formalin for reprocessing. This procedure dramatically improves the results in at least 90% of cases. The excellent results are probably due to the protective nature of the wax present in the adequately processed portions of the block. This insulates the tissue from the harmful effects of ethanol on the adequately processed portions of the tissue preventing the tissue from becoming hard and brittle (Johnson 2003). Johnson (2003) Histologic 36(1):21-22. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Thursday, 8 July 2010 1:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =oday is...I grossed in some fat that I need to routine process. I ha=e done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =the middle of my block. I fixed for 48 hours, but guess my section we=re too big and needed more. I want to fix for a little longer and rep=ocess the block. I know I need to melt it down, but then what? =I have done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=SCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From AnthonyH <@t> chw.edu.au Wed Jul 7 19:15:02 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jul 7 19:15:17 2010 Subject: [Histonet] DAB In-Reply-To: <4C3498DC.C0AD.00BF.0@inspection.gc.ca> Message-ID: Keri, For the free base of DAB you will need to dissolve it in N,N dimethyl formamide. The free base is poorly soluble in aqueous solutions. Substitute the formamide for the distilled water: 1. Weigh out 2g DAB in a fume hood 2. Dissolve in 10ml N,N dimethyl formamide 3. Label fifty 1ml reagent vials 4. Aliquot 0.1ml DAB solution in each tube 5. Freeze and Store at -20oC For use: Add 0.1ml DAB solution to 50ml buffer and add 50 microlitres of 30% hydrogen peroxide. I prefer to use the DAB tetrahydrochloride Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: Thursday, 8 July 2010 7:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB Hello Histonetters, I am currently trying to work out a new DAB protocol to be used with IHC on FFPE sections. I am testing three different protocols, and am having limited success. The first protocol requires me to make a DAB stock solution of 10mg in 1 ml dH20, which is then filtered and frozen immediately. This protocol suggests using DAB tetrahydrochloride XH20, but I am using the free base of DAB as that is what was on hand. The free base does not want to go into solution, but has produced some reaction in my IHC. My second protocol asks for 40mg DAB in 1.5 ml Tris buffer (0.05M, pH 7.6), to make my stock solution. I am again using the free base, and have been stirring since 11:30 am yesterday (MDT) with the result being a chocolate milk-like substance. My third protocol requires me to make up the DAB fresh each time I want to use it. This protocol also asks for the tetrahydrochloride form, but again I used the free base and did get some reaction. Does anyone have thoughts on about how to get the free base to go into solution without oxidizing, or thoughts about free base versus the other form of DAB? Thanks! Keri Keri Colwell Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell@inspection.gc.ca Telephone | T?l?phone: 403-382-5500 Facsimile | T?l?copieur: 403-382-5583 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From amosbrooks <@t> gmail.com Wed Jul 7 19:40:17 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Jul 7 19:40:26 2010 Subject: [Histonet] Rnase free slides? In-Reply-To: References: Message-ID: Hi Emily, As you said RNAases are everywhere, so chasing them all down would be akin to hunting the boogyman. The way I see it is since there is no realistic way of eliminating any contact at all with RNAase it really comes down to merely damage control. Start with gloves, you reduce the introduction of RNAase by a certain percentage. Wipe down all the surfaces that come in contact with the tissue with RNAase away, you reduced the percentage a bit further. Only use DEPC treated water and you reduce the percentage further and so on until it gets to be so labor intensive that it is non productive. You can hold your breath to reduce them too... don't laugh I found myself doing it unwittingly one day. So what happens if we scale it down to a workable level? Some RNAases will inevitably be introduced this will reduce the PCR yield some. If you are doing it a certain way and your yields are sufficient, it aint broke so don't fix it! So if you are using untreated slides and it's working, don't change a thing. That having been said I never assume anything is RNAase free. This includes the slides and any chemicals I use, previously opened or not. So if it doesn't take much effort to RNAase away treat something I usually do it. Otherwise I just chalk it up to acceptable yield loss. Incidentally I meant 30% sucrose when I said 30% in my last post. I've never done a side by side comparison of the difference between 30% sucrose in DEPC and OCT. There may be no appreciable difference at all. I merely mentioned it as an option. So a funny story: I had a researcher really giving me H-E-double hockey sticks wanting me to make absolutly sure there was no possibility of RNAase contamination. (Thus the holding my breath I mentioned earlier.) So I went into full OCD mode and decontaminated everything in RNAase away including the box I put the slides in. The researcher picked up the slides and after grilling me again about my technique he popped open the box (bare-handed, mind you) to check the slides out and ran his finger down the sides of the slides. I literally saw the RNAase bugs eating away all my hard work before my eyes. When I caught my breath I took a long lunch and went home early. Later that week I saw the researcher. He said the yield was great and I must have followed his advise well. That was a Scotch night! RNAase ... the boogeyman and Big Foot! Happy Wednesday, Amos On Wed, Jul 7, 2010 at 7:32 PM, Emily Sours wrote: > Am I wrong in assuming that the glass slides I get (which are in a > plastic box, wrapped in plastic) are not RNase free? They're > superfrost plus from Fisher. It hasn't been a problem for us to > assume so, but I'm wondering what other labs think about it. > We've never treated our slides with Rnase Away or rinsed in DEPC > water. We use DEPC water for in situ hybridization solutions. The in > situ dishes are sprayed with RNase away, then DEPC water. > Also use OCT when you section, it'll make your life so much easier! > It's assumed in my lab that if a chemical came in and hasn't been > opened, it's RNase free. To keep it RNase free, you always wear > gloves. Store your slides in a new box to keep them RNase free. > > RNases are everywhere on people, I assumed, and that was the problem. > I didn't think RNases are everywhere in the universe on every object. > But I could be wrong. Anyone else have an opinion? > > Emily > -- > Dark Pictures, thrones and stones that pilgrims kiss > And poems that take a thousand years to die > But ape the immortality of this > Red label on a little butterfly. > > -Vladimir Nabokov, concluding stanza of ?A Discovery? 1941. > From LSebree <@t> uwhealth.org Thu Jul 8 07:51:21 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Jul 8 07:51:26 2010 Subject: [Histonet] Vendors & others: X-reactivity between HSV I & HSV II? Message-ID: <8C023B4AB999614BA4791BAEB26E2738399EE8@UWHC-MAIL01.uwhis.hosp.wisc.edu> Having switched from an HSV I & II antibody cocktail to separate antibodies for HSV I and HSV II, we have found (and the manufacturer has confirmed) that the two separate antibodies cross react. Does anyone know of antibodies against HSV I and HSV II that do not cross react? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From histotech <@t> imagesbyhopper.com Thu Jul 8 08:02:22 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Jul 8 08:02:41 2010 Subject: [Histonet] Reprocess In-Reply-To: Message-ID: What Tony suggests is similar to what we do for reprocessing. If we have a block that we embedded and attempted to section, only to discover that it's not fixed well enough, we do the following: 1) print a new processing cassette 2) using a "dull" knife (we have an old, dull steak knife), run the blade between the edge of the block and the cassette and "shave" the embedded tissue off the cassette. 3) take the wax/tissue, place in the new cassette and process. No need to melt it down or run it back down from xylene to formalin. The moist tissue is already exposed and seems to re-process just fine. Your mileage may vary! Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Wednesday, July 07, 2010 7:48 PM To: sgoebel@xbiotech.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocess You do not need to rehydrate the blocks back to water, While the tissue is still hot (ie the wax is still molten) blot the tissue dry, place it back in its cassette and place the cassette in 10% formalin for reprocessing. This procedure dramatically improves the results in at least 90% of cases. The excellent results are probably due to the protective nature of the wax present in the adequately processed portions of the block. This insulates the tissue from the harmful effects of ethanol on the adequately processed portions of the tissue preventing the tissue from becoming hard and brittle (Johnson 2003). Johnson (2003) Histologic 36(1):21-22. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Thursday, 8 July 2010 1:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =oday is...I grossed in some fat that I need to routine process. I ha=e done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =the middle of my block. I fixed for 48 hours, but guess my section we=re too big and needed more. I want to fix for a little longer and rep=ocess the block. I know I need to melt it down, but then what? =I have done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=SCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **************************************************************************** ***** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.439 / Virus Database: 271.1.1/2987 - Release Date: 07/07/10 06:36:00 From Sharon.Davis-Devine <@t> carle.com Thu Jul 8 08:51:10 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Thu Jul 8 08:53:52 2010 Subject: [Histonet] Peloris Users Message-ID: To all you Peloris users, here is my delimma. When we follow the machines prompt to change the solutions because of purity and we then process small biopsies on the machine, it overprocesses the specimens. So the staff have been overrriding the prompt to change the solutions causing specimens to be processed in impure solutions causing a host of other problems. Have any of you out there with Peloris experience had this problem? Is it a machine problem that should be fixed or human error due to overriding the prompts? I am new to this position and have very little experience with this issue so any help or suggestions will be greatly appreiciated. Thank you. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From JCBRITTON <@t> Cheshire-Med.COM Thu Jul 8 11:03:08 2010 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Thu Jul 8 11:03:18 2010 Subject: [Histonet] Peloris Users In-Reply-To: References: Message-ID: We have helped that problem by being sure there is adequate graded alcohols on the Peloris. There must be between 60-70%, 80-90%, and 95% before you have the 100% alcohols. If the concentration's are too high there is inadequate infiltration of the tissue's. We usually only use the 2 hour and the 8 hour processing factory default. Unless, we have a stat. We have also noticed if you wait until Prompted to change the lowest alcohol at 51% we see degradation in our staining as well. We are not letting it get below 58-60% before we change an alcohol out. I hope this helps, Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 603-354-5454 ext.2454 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, July 08, 2010 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris Users To all you Peloris users, here is my delimma. When we follow the machines prompt to change the solutions because of purity and we then process small biopsies on the machine, it overprocesses the specimens. So the staff have been overrriding the prompt to change the solutions causing specimens to be processed in impure solutions causing a host of other problems. Have any of you out there with Peloris experience had this problem? Is it a machine problem that should be fixed or human error due to overriding the prompts? I am new to this position and have very little experience with this issue so any help or suggestions will be greatly appreiciated. Thank you. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From foreightl <@t> gmail.com Thu Jul 8 11:33:20 2010 From: foreightl <@t> gmail.com (Pat Laurie) Date: Thu Jul 8 11:33:25 2010 Subject: [Histonet] Peloris Users In-Reply-To: References: Message-ID: The machine will work well only if it is set up well and the staff follows all of the prompts. We set up ours with two stations of 80% alcohol in the beginning to make sure it starts with a good graded series of alcohols. Another thing to look at is the biopsy pad percentage. Setting it right will help the machine more accurately figure out the actual percentage of the alcohols. If the staff isn't following the prompts it can really wreck the processing. The machine is relying on the calculations to perform a proper processing schedule. On Thu, Jul 8, 2010 at 9:03 AM, Josie Britton wrote: > > > We have helped that problem by being sure there is adequate graded > alcohols on the Peloris. There must be between 60-70%, 80-90%, and 95% > before you have the 100% alcohols. If the concentration's are too high > there is inadequate infiltration of the tissue's. We usually only use > the 2 hour and the 8 hour processing factory default. Unless, we have a > stat. We have also noticed if you wait until Prompted to change the > lowest alcohol at 51% we see degradation in our staining as well. We > are not letting it get below 58-60% before we change an alcohol out. > > > > I hope this helps, > > > > Josie Britton HT > > Cheshire Medical Center > > 580 Court Street > > Keene, NH 03431 > > 603-354-5454 ext.2454 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Sharon.Davis-Devine > Sent: Thursday, July 08, 2010 9:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Peloris Users > > > > To all you Peloris users, here is my delimma. When we follow the > machines prompt to change the solutions because of purity and we then > process small biopsies on the machine, it overprocesses the specimens. > So the staff have been overrriding the prompt to change the solutions > causing specimens to be processed in impure solutions causing a host of > other problems. Have any of you out there with Peloris experience had > this problem? Is it a machine problem that should be fixed or human > error due to overriding the prompts? I am new to this position and have > very little experience with this issue so any help or suggestions will > be greatly appreiciated. Thank you. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology-Histology Supervisor > > Carle Foundation Hospital > > Laboratory and Pathology Services > > 611 West Park Street > > Urbana, Illinois 61801 > > 217-383-3572 > > sharon.davis-devine@carle.com > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE: This electronic message, including any attachments, > is for the sole use of the intended recipients and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by electronic mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com From mward <@t> wfubmc.edu Thu Jul 8 11:58:45 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu Jul 8 11:58:48 2010 Subject: [Histonet] PGP 9.5 antibody on Bond stainer Message-ID: <61135F0455D33347B5AAE209B903A30433DEBAE3@EXCHVS2.medctr.ad.wfubmc.edu> I have been asked about bringing this antibody in house and I was wondering if anyone is staining for this using a Leica Bond stainer. Any advice, tips, etc. would be appreciated. We are looking at getting the antibody from Dako, cat# Z 5116. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 From STapper <@t> smdc.org Thu Jul 8 12:47:58 2010 From: STapper <@t> smdc.org (Tapper, Sheila J.) Date: Thu Jul 8 12:48:10 2010 Subject: [Histonet] Peloris Users In-Reply-To: References: Message-ID: I you are going to choose to override the prompts, you had best revert back to a schedule of changing solutions on a rotating schedule. You probably had something like that in place with your previous processor. I would call Leica to have them send out someone to help get your processing optimized, and train your staff on how to set the parameters for your solutions. They are very interested in keeping their customers happy. Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, July 08, 2010 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris Users To all you Peloris users, here is my delimma. When we follow the machines prompt to change the solutions because of purity and we then process small biopsies on the machine, it overprocesses the specimens. So the staff have been overrriding the prompt to change the solutions causing specimens to be processed in impure solutions causing a host of other problems. Have any of you out there with Peloris experience had this problem? Is it a machine problem that should be fixed or human error due to overriding the prompts? I am new to this position and have very little experience with this issue so any help or suggestions will be greatly appreiciated. Thank you. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From jlhowery <@t> yrmc.org Thu Jul 8 13:51:07 2010 From: jlhowery <@t> yrmc.org (jeff) Date: Thu Jul 8 13:51:22 2010 Subject: [Histonet] AP Computer Systems Message-ID: <000601cb1ece$86516a90$3394640a@yrmc.org> We are currently looking at a overall computer system for our Hospital. My point of this question is has anyone worked with and their feelings about the following vendors for the AP application: Meditech Cerner Quadra Med Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jlhowery <@t> yrmc.org Thu Jul 8 13:56:53 2010 From: jlhowery <@t> yrmc.org (jeff) Date: Thu Jul 8 13:56:57 2010 Subject: [Histonet] Histo bath Message-ID: <000601cb1ecf$545807f0$3394640a@yrmc.org> I am looking for a condenser cooling fan motor for my Histo -Bath. Thank you for any Help . Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sharonin1 <@t> gmail.com Thu Jul 8 14:31:58 2010 From: sharonin1 <@t> gmail.com (sharon in) Date: Thu Jul 8 14:32:26 2010 Subject: [Histonet] Helf- strange IF result Message-ID: Hi, I am detecting Arc (Activity regulated cytoskeletal protein) protein in rat brain slices. I perfuse transcardially the animal with 0.1M PBS and than 4% PFA with 5% sucrose. Post fixation in 30% sucrose 1% PFA. In some cases when i take out the brain after the perfusion i make small cut or incision with the skull in the motor cortex, usually at the right side of the brain. I stain coronal 40um brain slices with primary antibody (from rabbit , santa cruz) and secondary anti rabbit Alexa488. I found very strong nuclear staining in the area around the cut in the motor cortex something like 100 um from each side. Do somebody has any idea what is the source of that staining? Generally the signal of Arc is in the nucleus and dendrites in other brain regions. I have very consistent results with Arc expression in the brain regions i am working on. The activation around the cutting area makes me worry a little. Thank you very much. From Laura.Miller <@t> leica-microsystems.com Thu Jul 8 16:48:53 2010 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Thu Jul 8 16:49:04 2010 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 07/08/2010 and will not return until 07/09/2010. I am out of the office for the rest of today. I will reply tomorrow, Friday, July 9th. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From DianaRip1 <@t> aol.com Thu Jul 8 20:09:41 2010 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Thu Jul 8 20:09:49 2010 Subject: [Histonet] PGP 9.5 Frozen Tissue Message-ID: <4a71f.2740e446.3967d0d5@aol.com> Is anyone out there doing the PGP 9.5 on fresh or frozen tissue? From sgoebel <@t> xbiotech.com Fri Jul 9 09:15:13 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Jul 9 09:15:21 2010 Subject: [Histonet] VIP 2000 Message-ID: <20100709071513.9e2d9aa830e8449a2412eb1e4f2f067e.77a782036c.wbe@email04.secureserver.net> Hello all, I recently purchased a refurbished VIP. 3rd time I have run it, but wait...diagnose flas error code 73. Of course the manual doesn't say what to fix it, and I can't seem to find anything online either. this is an older machine and lots of you have used it, was ju wondering if anyone knew what the heck diagnose code 73 is? Th Sarah Goebel, B.A., HT (ASCP)< Histotechnician XBiotech USA Inc. 8201 E < (512)386-51 From pruegg <@t> ihctech.net Fri Jul 9 09:26:18 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jul 9 09:27:16 2010 Subject: SPAM-LOW: RE: [Histonet] Reprocess In-Reply-To: References: Message-ID: <6CEEF65C14414251AA4D2424461FE035@prueggihctechlt> I do something similar but I do melt the block down and dab out the melted paraffin with paper towel, put the melted tissue in a cassette and reprocess, it works a charm. No need to remove paraffin. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, July 08, 2010 7:02 AM To: 'Tony Henwood'; sgoebel@xbiotech.com; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] Reprocess What Tony suggests is similar to what we do for reprocessing. If we have a block that we embedded and attempted to section, only to discover that it's not fixed well enough, we do the following: 1) print a new processing cassette 2) using a "dull" knife (we have an old, dull steak knife), run the blade between the edge of the block and the cassette and "shave" the embedded tissue off the cassette. 3) take the wax/tissue, place in the new cassette and process. No need to melt it down or run it back down from xylene to formalin. The moist tissue is already exposed and seems to re-process just fine. Your mileage may vary! Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Wednesday, July 07, 2010 7:48 PM To: sgoebel@xbiotech.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocess You do not need to rehydrate the blocks back to water, While the tissue is still hot (ie the wax is still molten) blot the tissue dry, place it back in its cassette and place the cassette in 10% formalin for reprocessing. This procedure dramatically improves the results in at least 90% of cases. The excellent results are probably due to the protective nature of the wax present in the adequately processed portions of the block. This insulates the tissue from the harmful effects of ethanol on the adequately processed portions of the tissue preventing the tissue from becoming hard and brittle (Johnson 2003). Johnson (2003) Histologic 36(1):21-22. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Thursday, 8 July 2010 1:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =oday is...I grossed in some fat that I need to routine process. I ha=e done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =the middle of my block. I fixed for 48 hours, but guess my section we=re too big and needed more. I want to fix for a little longer and rep=ocess the block. I know I need to melt it down, but then what? =I have done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=SCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **************************************************************************** ***** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.439 / Virus Database: 271.1.1/2987 - Release Date: 07/07/10 06:36:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 41dmb41 <@t> gmail.com Fri Jul 9 10:35:06 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Fri Jul 9 10:35:31 2010 Subject: [Histonet] VIP 2000 In-Reply-To: <20100709071513.9e2d9aa830e8449a2412eb1e4f2f067e.77a782036c.wbe@email04.secureserver.net> References: <20100709071513.9e2d9aa830e8449a2412eb1e4f2f067e.77a782036c.wbe@email04.secureserver.net> Message-ID: You can always call Sakura... they'll at least tell you what the codes means... Drew On Fri, Jul 9, 2010 at 10:15, wrote: > > Hello all, > > I recently purchased a refurbished VIP. ; This would have been the > 3rd time I have run it, but wait...diagnose flas hes and I get an > error code 73. Of course the manual doesn't say what this is or how > to fix it, and I can't seem to find anything online either. I know > this is an older machine and lots of you have used it, was ju st > wondering if anyone knew what the heck diagnose code 73 is? > > Th anks > > Sarah Goebel, B.A., HT (ASCP)< /div> > Histotechnician > XBiotech USA Inc. > 8201 E ast Riverside Dr. Bldg 4 Suite 100 > < i>A ustin, Texas 78744 > (512)386-51 07 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sbaldwin <@t> mhhcc.org Fri Jul 9 10:50:02 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Fri Jul 9 10:50:46 2010 Subject: [Histonet] Correct CPT? Message-ID: Hey histoland When we do an FNA we sometimes have a cell block and we bill CPT 88160 and 88305 These are sometimes kicked back to us and we put a 59 modifier on the 88305 then it goes thru. Are we doing this correct ? Any advice? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From settembr <@t> umdnj.edu Fri Jul 9 11:14:08 2010 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Jul 9 11:14:30 2010 Subject: [Histonet] Anybody running RCC on Bond Message-ID: Is anybody running RCC (Renal Cell Carcinoma) on Leica's Bond Max on human tissue? I am trying to bring it into our lab. I can't seem to get it working. Normal tissue is staining but not tumor. I am using Lieca's concentrated antibody. Help. What is your Vendor? Thank you, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> cvmc.org Fri Jul 9 11:25:25 2010 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Fri Jul 9 11:25:30 2010 Subject: [Histonet] VIP 2000 In-Reply-To: Message-ID: Error code 73: Symptom: Rotary valve does not stop at the required station after 2 times rotation Probable cause: 1. Photointerrupter defective 2. Positioning disc broken 3. Photointerrupter for position detection disturbed by strong light source Remedy: 1. Replace photointerrupter PC board 2. Replace positioning disc 3. Eliminate light source Just found this information in my older VIP E300 manual. Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From wlecorch <@t> rwjuhh.edu Fri Jul 9 12:18:42 2010 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Fri Jul 9 12:18:46 2010 Subject: [Histonet] Vol 80, Issue 11 Correct CPT? (Sara Baldwin/mhhcc.org) Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71AD89379C3@HAMEXMBA.rwjham.local> You can bill 88173 for the FNA and 88305 for the cell block no modifiers necessary, in addition if you perform onsite adequacy you can bill 88172 Bill Lecorchick Cytology Prep.Tech. 609-584-5128 Fax 609-584-6439 wlecorch@rwjuhh.edu www.rwjhamilton.org From rsrichmond <@t> gmail.com Fri Jul 9 12:28:40 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Jul 9 12:28:46 2010 Subject: [Histonet] Re: Histobath Message-ID: Jeff Howery is >>looking for a condenser cooling fan motor for my Histo -Bath.<< We've had a good bit of discussion over the years about Histobath and the coolant used in it. I think this stuff is still in the Histonet archives. Briefly, Histobath is no longer made. There are some new products that replace it. And there is a non-flammable coolant that's a lot safer than acetone or 2-methylbutane. Bob Richmond Samurai Pathologist Knoxville TN From brianj18 <@t> u.washington.edu Fri Jul 9 12:38:08 2010 From: brianj18 <@t> u.washington.edu (Brian Johnson) Date: Fri Jul 9 12:39:00 2010 Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 11 In-Reply-To: <201007091701.o69H1cGQ013467@cg77.u.washington.edu> References: <201007091701.o69H1cGQ013467@cg77.u.washington.edu> Message-ID: Hi Karol I just wanted to follow up with you regarding the Fluorescent secondary antibodies and if you needed to order them or not. Can I come by your lab to chat for a minute? Where are you located? Brian Brian Johnson Research Scientist U.W. at South Lake Union, N310 HIC/Comparative Pathology Program 815 Mercer St Seattle, WA 98109 Work Phone: 206-685-6517 Work E-mail: brianj18@uw.edu On Jul 9, 2010, at 10:01 AM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Peloris Users (Tapper, Sheila J.) > 2. AP Computer Systems (jeff) > 3. Histo bath (jeff) > 4. Helf- strange IF result (sharon in) > 5. Laura Miller is Out of the Office. > (Laura.Miller@leica-microsystems.com) > 6. PGP 9.5 Frozen Tissue (DianaRip1@aol.com) > 7. VIP 2000 (sgoebel@xbiotech.com) > 8. RE: SPAM-LOW: RE: [Histonet] Reprocess (Patsy Ruegg) > 9. Re: VIP 2000 (Drew Meyer) > 10. Correct CPT? (Sara Baldwin/mhhcc.org) > 11. Anybody running RCC on Bond (Dana Settembre) > 12. RE: VIP 2000 (Bell, Lynne) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 8 Jul 2010 12:47:58 -0500 > From: "Tapper, Sheila J." > Subject: RE: [Histonet] Peloris Users > To: "Sharon.Davis-Devine" , > > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > I you are going to choose to override the prompts, you had best revert > back to a schedule of changing solutions on a rotating schedule. > You probably had something like that in place with your previous > processor. I would call Leica to have them send out someone to help get > your processing optimized, and train your staff on how to set the > parameters for your solutions. They are very interested in keeping > their customers happy. > > Sheila > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Sharon.Davis-Devine > Sent: Thursday, July 08, 2010 8:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Peloris Users > > To all you Peloris users, here is my delimma. When we follow the > machines prompt to change the solutions because of purity and we then > process small biopsies on the machine, it overprocesses the specimens. > So the staff have been overrriding the prompt to change the solutions > causing specimens to be processed in impure solutions causing a host of > other problems. Have any of you out there with Peloris experience had > this problem? Is it a machine problem that should be fixed or human > error due to overriding the prompts? I am new to this position and have > very little experience with this issue so any help or suggestions will > be greatly appreiciated. Thank you. > > Sharon Davis-Devine, CT (ASCP) > Cytology-Histology Supervisor > Carle Foundation Hospital > Laboratory and Pathology Services > 611 West Park Street > Urbana, Illinois 61801 > 217-383-3572 > sharon.davis-devine@carle.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. > > > > > > ------------------------------ > > Message: 2 > Date: Thu, 8 Jul 2010 11:51:07 -0700 > From: "jeff" > Subject: [Histonet] AP Computer Systems > To: > Message-ID: <000601cb1ece$86516a90$3394640a@yrmc.org> > Content-Type: text/plain; charset="iso-8859-1" > > We are currently looking at a overall computer system for our Hospital. My point of this question is has anyone worked with and their feelings about the following vendors for the AP application: > Meditech > Cerner > Quadra Med > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > > > ------------------------------ > > Message: 3 > Date: Thu, 8 Jul 2010 11:56:53 -0700 > From: "jeff" > Subject: [Histonet] Histo bath > To: > Message-ID: <000601cb1ecf$545807f0$3394640a@yrmc.org> > Content-Type: text/plain; charset="iso-8859-1" > > I am looking for a condenser cooling fan motor for my Histo -Bath. Thank you for any Help . > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > > > ------------------------------ > > Message: 4 > Date: Thu, 8 Jul 2010 22:31:58 +0300 > From: sharon in > Subject: [Histonet] Helf- strange IF result > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > > I am detecting Arc (Activity regulated cytoskeletal protein) protein in rat > brain slices. > I perfuse transcardially the animal with 0.1M PBS and than 4% PFA with 5% > sucrose. Post fixation in 30% sucrose 1% PFA. In some cases when i take out > the brain after the perfusion i make small cut or incision with the skull in > the motor cortex, usually at the right side of the brain. I stain coronal > 40um brain slices with primary antibody (from rabbit , santa cruz) and > secondary anti rabbit Alexa488. I found very strong nuclear staining in the > area around the cut in the motor cortex something like 100 um from each > side. Do somebody has any idea what is the source of that staining? > Generally the signal of Arc is in the nucleus and dendrites in other brain > regions. I have very consistent results with Arc expression in the brain > regions i am working on. The activation around the cutting area makes me > worry a little. > Thank you very much. > > > ------------------------------ > > Message: 5 > Date: Thu, 8 Jul 2010 16:48:53 -0500 > From: Laura.Miller@leica-microsystems.com > Subject: [Histonet] Laura Miller is Out of the Office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 07/08/2010 and will not return until > 07/09/2010. > > I am out of the office for the rest of today. I will reply tomorrow, > Friday, July 9th. > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > > > > ------------------------------ > > Message: 6 > Date: Thu, 8 Jul 2010 21:09:41 EDT > From: DianaRip1@aol.com > Subject: [Histonet] PGP 9.5 Frozen Tissue > To: histonet@lists.utsouthwestern.edu > Message-ID: <4a71f.2740e446.3967d0d5@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > Is anyone out there doing the PGP 9.5 on fresh or frozen tissue? > > > ------------------------------ > > Message: 7 > Date: Fri, 09 Jul 2010 07:15:13 -0700 > From: sgoebel@xbiotech.com > Subject: [Histonet] VIP 2000 > To: histonet@lists.utsouthwestern.edu > Message-ID: > <20100709071513.9e2d9aa830e8449a2412eb1e4f2f067e.77a782036c.wbe@email04.secureserver.net> > > Content-Type: text/plain; charset="utf-8" > > > Hello all, > > I recently purchased a refurbished VIP. 3rd time I have run it, but wait...diagnose flas error code 73. Of course the manual doesn't say what to fix it, and I can't seem to find anything online either. this is an older machine and lots of you have used it, was ju wondering if anyone knew what the heck diagnose code 73 is? > > Th > Sarah Goebel, B.A., HT (ASCP)< Histotechnician > XBiotech USA Inc. > 8201 E < (512)386-51 > > ------------------------------ > > Message: 8 > Date: Fri, 9 Jul 2010 08:26:18 -0600 > From: "Patsy Ruegg" > Subject: RE: SPAM-LOW: RE: [Histonet] Reprocess > To: , "'Tony Henwood'" > , , > > Message-ID: <6CEEF65C14414251AA4D2424461FE035@prueggihctechlt> > Content-Type: text/plain; charset="us-ascii" > > I do something similar but I do melt the block down and dab out the melted > paraffin with paper towel, put the melted tissue in a cassette and > reprocess, it works a charm. No need to remove paraffin. > > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histotech@imagesbyhopper.com > Sent: Thursday, July 08, 2010 7:02 AM > To: 'Tony Henwood'; sgoebel@xbiotech.com; histonet@lists.utsouthwestern.edu > Subject: SPAM-LOW: RE: [Histonet] Reprocess > > What Tony suggests is similar to what we do for reprocessing. If we have a > block that we embedded and attempted to section, only to discover that it's > not fixed well enough, we do the following: > > 1) print a new processing cassette > 2) using a "dull" knife (we have an old, dull steak knife), run the blade > between the edge of the block and the cassette and "shave" the embedded > tissue off the cassette. > 3) take the wax/tissue, place in the new cassette and process. > > No need to melt it down or run it back down from xylene to formalin. The > moist tissue is already exposed and seems to re-process just fine. > > Your mileage may vary! > > Michelle > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood > Sent: Wednesday, July 07, 2010 7:48 PM > To: sgoebel@xbiotech.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Reprocess > > > You do not need to rehydrate the blocks back to water, > > While the tissue is still hot (ie the wax is still molten) blot the tissue > dry, place it back in its cassette and place the cassette in 10% formalin > for reprocessing. This procedure dramatically improves the results in at > least 90% of cases. The excellent results are probably due to the protective > nature of the wax present in the adequately processed portions of the block. > This insulates the tissue from the harmful effects of ethanol on the > adequately processed portions of the tissue preventing the tissue from > becoming hard and brittle (Johnson 2003). > > Johnson (2003) Histologic 36(1):21-22. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & > Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > sgoebel@xbiotech.com > Sent: Thursday, 8 July 2010 1:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Reprocess > > > > Hello all!! Hope everyone had a happy 4th!! Question =oday is...I > grossed in some fat that I need to routine process. I ha=e done > this in the past with extra fixation and no problem? This tim= > however it didn't fix all the way through and I have oily unfixed fat > in =the middle of my block. I fixed for 48 hours, but guess my > section we=re too big and needed more. I want to fix for a little > longer and rep=ocess the block. I know I need to melt it down, but > then what? =I have done this before, it's just my brain is getting > older =) > > Thanks in advance!! > > Sarah Goebel, B.A., HT (=SCP) > > Histotechnician > > XBiotech USA Inc. > > > 8201 East Riverside Dr. Bldg 4 Suite 100 > > Austin, Texas 78744 > > (512)386-5107 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > **************************************************************************** > ***** > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Childrens Hospital at > Westmead accepts no liability for any consequential damage resulting from > email containing computer viruses. > **************************************************************************** > ***** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.439 / Virus Database: 271.1.1/2987 - Release Date: 07/07/10 > 06:36:00 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 9 > Date: Fri, 9 Jul 2010 11:35:06 -0400 > From: Drew Meyer <41dmb41@gmail.com> > Subject: Re: [Histonet] VIP 2000 > To: sgoebel@xbiotech.com > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > You can always call Sakura... they'll at least tell you what the codes > means... > > Drew > > On Fri, Jul 9, 2010 at 10:15, wrote: > >> >> Hello all, >> >> I recently purchased a refurbished VIP. ; This would have been the >> 3rd time I have run it, but wait...diagnose flas hes and I get an >> error code 73. Of course the manual doesn't say what this is or how >> to fix it, and I can't seem to find anything online either. I know >> this is an older machine and lots of you have used it, was ju st >> wondering if anyone knew what the heck diagnose code 73 is? >> >> Th anks >> >> Sarah Goebel, B.A., HT (ASCP)< /div> >> Histotechnician >> XBiotech USA Inc. >> 8201 E ast Riverside Dr. Bldg 4 Suite 100 >> < i>A ustin, Texas 78744 >> (512)386-51 07 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ------------------------------ > > Message: 10 > Date: Fri, 9 Jul 2010 11:50:02 -0400 > From: "Sara Baldwin/mhhcc.org" > Subject: [Histonet] Correct CPT? > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=ISO-8859-1 > > Hey histoland > When we do an FNA we sometimes have a cell block and we bill CPT 88160 and 88305 > These are sometimes kicked back to us and we put a 59 modifier on the 88305 then it goes thru. Are we doing this correct ? Any advice? > > Thanks > Pathology Supervisor > Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center > sbaldwin@mhhcc.org > Ph 812-482-0210, 482-0216, Fax 812-482-0232, > Pager 812-481-0897 > Confidential information, Authorized use only. > > > > ------------------------------ > > Message: 11 > Date: Fri, 09 Jul 2010 12:14:08 -0400 > From: "Dana Settembre" > Subject: [Histonet] Anybody running RCC on Bond > To: "Alyssa Peterson" , > , "Dana Settembre" > > Cc: Catherine Susan Delia , Neena Mirani > > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Is anybody running RCC (Renal Cell Carcinoma) on Leica's Bond Max on > human > tissue? > I am trying to bring it into our lab. > I can't seem to get it working. > Normal tissue is staining but not tumor. > I am using Lieca's concentrated antibody. > Help. > What is your Vendor? > > Thank you, > > Dana Settembre, HT ASCP > Immunohistochemistry Lab > UMDNJ - University Hospital > Newark, NJ USA > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 12 > Date: Fri, 9 Jul 2010 12:25:25 -0400 > From: "Bell, Lynne" > Subject: RE: [Histonet] VIP 2000 > To: "sgoebel@xbiotech.com" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Error code 73: > Symptom: Rotary valve does not stop at the required station after 2 times rotation > Probable cause: 1. Photointerrupter defective > 2. Positioning disc broken > 3. Photointerrupter for position detection disturbed by strong light source > Remedy: 1. Replace photointerrupter PC board > 2. Replace positioning disc > 3. Eliminate light source > > Just found this information in my older VIP E300 manual. > > Lynne A. Bell, HT (ASCP) > Technical Specialist, Histology > Central Vermont Medical Center > 130 Fisher Road > Barre, VT 05641 > 802-371-4923 > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 80, Issue 11 > **************************************** From sfeher <@t> CMC-NH.ORG Fri Jul 9 12:46:45 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Jul 9 12:46:50 2010 Subject: [Histonet] Correct CPT? In-Reply-To: References: Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B755D9@exchange.cmc-nh.org> Sara, We bill 88173 for our FNA's along with the 88305 when we do a cell block. We have not had an issue with any of these getting kicked back. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Friday, July 09, 2010 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Correct CPT? Hey histoland When we do an FNA we sometimes have a cell block and we bill CPT 88160 and 88305 These are sometimes kicked back to us and we put a 59 modifier on the 88305 then it goes thru. Are we doing this correct ? Any advice? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From areed46254 <@t> yahoo.com Fri Jul 9 12:50:12 2010 From: areed46254 <@t> yahoo.com (amy reed) Date: Fri Jul 9 12:50:16 2010 Subject: [Histonet] posting Message-ID: <126924.71328.qm@web63105.mail.re1.yahoo.com> HT needed for high volume Mohs Practice in Indianapolis, IN. Experience in frozen section preferred, HT?(ASCP) preferred. ? From matt <@t> techoneweb.com Fri Jul 9 13:31:34 2010 From: matt <@t> techoneweb.com (matt@techoneweb.com) Date: Fri Jul 9 13:31:38 2010 Subject: [Histonet] re:VIP 2000 Message-ID: <186ba77721b197074c5dc64641a89c11.squirrel@www.techoneweb.com> Hey Sarah The error code is a rotary valve error. "Rotary valve does not stop at the required station after 2 times". My guess is that you have a bad rotary valve sensor board. It could also be the sensor disk. Best Matt Mincer Tech One Biomedical Services 708-383-6040 X 10 www.techoneweb.com From jphistology <@t> gmail.com Fri Jul 9 14:00:49 2010 From: jphistology <@t> gmail.com (Joao Pessoa) Date: Fri Jul 9 14:00:51 2010 Subject: [Histonet] Formalin and Xylene odor containment Message-ID: All, We have recently been using a new piece of demo equipment to control formalin, xylene and other odors. The unit is an air purifier from BioZone Scientific. Our ventilation is good in all areas of our lab and we have always been found to be well below the particulate count allotment for xylene, formalin, and other compounds. However, many area of the lab still had a heavy chemical smell (like a beauty salon, they say). This smell goes away with the BioZone unit, which uses UV light and ozone to break down the organic compounds. Does anyone else has experience with BioZone? We are thinking about buying it, but I wanted to hear the opinion of others. Cheers, Joao Histo Tech From dellav <@t> musc.edu Fri Jul 9 14:09:25 2010 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Jul 9 14:09:31 2010 Subject: [Histonet] Formalin and Xylene odor containment In-Reply-To: References: Message-ID: I don't have any expertise in this area however I recall reading about the controversies of ozone generators. You would do well to do a bit of internet research before you take this leap. If your facility has a safety officer I'd definitely determine if there is an institution position on ozone generators. Here is one link for information but you shouldn't have difficulty coming up with others. http://www.epa.gov/iaq/pubs/ozonegen.html Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joao Pessoa Sent: Friday, July 09, 2010 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin and Xylene odor containment All, We have recently been using a new piece of demo equipment to control formalin, xylene and other odors. The unit is an air purifier from BioZone Scientific. Our ventilation is good in all areas of our lab and we have always been found to be well below the particulate count allotment for xylene, formalin, and other compounds. However, many area of the lab still had a heavy chemical smell (like a beauty salon, they say). This smell goes away with the BioZone unit, which uses UV light and ozone to break down the organic compounds. Does anyone else has experience with BioZone? We are thinking about buying it, but I wanted to hear the opinion of others. Cheers, Joao Histo Tech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Fri Jul 9 14:14:02 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Jul 9 14:14:09 2010 Subject: [Histonet] Formalin and Xylene odor containment In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2FA@EXCHMBC2.ad.ah.local> Great advice Vinnie, At a prior job our safety manager found there to be more problems with ozone than what we were trying to fix. Jan mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Friday, July 09, 2010 2:09 PM To: 'Joao Pessoa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin and Xylene odor containment I don't have any expertise in this area however I recall reading about the controversies of ozone generators. You would do well to do a bit of internet research before you take this leap. If your facility has a safety officer I'd definitely determine if there is an institution position on ozone generators. Here is one link for information but you shouldn't have difficulty coming up with others. http://www.epa.gov/iaq/pubs/ozonegen.html Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joao Pessoa Sent: Friday, July 09, 2010 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin and Xylene odor containment All, We have recently been using a new piece of demo equipment to control formalin, xylene and other odors. The unit is an air purifier from BioZone Scientific. Our ventilation is good in all areas of our lab and we have always been found to be well below the particulate count allotment for xylene, formalin, and other compounds. However, many area of the lab still had a heavy chemical smell (like a beauty salon, they say). This smell goes away with the BioZone unit, which uses UV light and ozone to break down the organic compounds. Does anyone else has experience with BioZone? We are thinking about buying it, but I wanted to hear the opinion of others. Cheers, Joao Histo Tech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. 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From LGrow <@t> coh.org Fri Jul 9 15:09:31 2010 From: LGrow <@t> coh.org (Grow, Leah) Date: Fri Jul 9 15:11:00 2010 Subject: [Histonet] unsubscribe References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2FA@EXCHMBC2.ad.ah.local> Message-ID: <07D1367AA1E6384A9EA6B1AD7D7DC0AC64B3AF@EXCH-VS3.coh.org> --------------------------------------------------------------------- SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. --------------------------------------------------------------------- From JefThompson <@t> salud.unm.edu Fri Jul 9 16:21:47 2010 From: JefThompson <@t> salud.unm.edu (Jeffrey Thompson) Date: Fri Jul 9 16:21:52 2010 Subject: [Histonet] air dry or heat dry Message-ID: <4C373E8B0200004D000C9B71@hsc-iagate1.health.unm.edu> Hello, We have an ongoing debate in our lab regarding the relative virtue of heat drying slides vs RT air drying them. Tissues: 4% paraformaldehyde perfused rat brains frozen in OCT blocks Sections: 10 microns on a cryostat (at approx -25 C) Slides: Superfrost Plus The slides are stored in slide boxes at -80 C until staining. When staining the heat dry faction (wanting to avoid icy slides) put their slide boxes straight out of the freezeer into a 50 C oven for 30 minutes before taking out slides to stain. then the box goes back to the freezer until the next round of staining, The air dry group feels that cooking the antigens repeatedly at 50 C is problematic so they take the frosty slides out their slide boxes and return them ASAP to the freezer. The slides to be stained are air dried in the fume hood for about 30 min. A third, middle of the road, person takes her slides out of the cold boxes and then puts the slides in a 60 C oven for 30 minutes. For all three groups then, the slides are given a PAP pen border to prepare for IHC and when the pen solution is dry, 10 minutes in acetone and the remainder of the staining procedure. So my question is: Who is using the best technique? Another hotly debated topic is wether it is advisable to put a drop of PBS on the slide before 'sticking' the section to prevent folds in the sections.. Any opinions are appreciated. Thanks, Jeff From saby_joseph_a <@t> yahoo.com Fri Jul 9 16:50:22 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Fri Jul 9 16:50:27 2010 Subject: [Histonet] re:VIP 2000 In-Reply-To: <186ba77721b197074c5dc64641a89c11.squirrel@www.techoneweb.com> References: <186ba77721b197074c5dc64641a89c11.squirrel@www.techoneweb.com> Message-ID: <87814.10576.qm@web114405.mail.gq1.yahoo.com> Sarah- There is another possibility.? If you use formalin in the first station, you should rinse out the first 2 containers when changing the processor and?fill them with warm water.? You can then?perform a warm water flush.? This is setting up a program with 1 minute in each of the first 2 stations, causing the warm water to be pumped in and pumped out.? Residual formaldehyde can crystallize on the rotary valve and cause the issue you are now experiencing.? A service tech can remove the rotary valve, clean it and grease it, and get you back functioning.? This would be a part of what your preventative maintenance should be doing. Good luck! Joe Saby, BA HT ________________________________ From: "matt@techoneweb.com" To: histonet@lists.utsouthwestern.edu Sent: Fri, July 9, 2010 2:31:34 PM Subject: [Histonet] re:VIP 2000 Hey Sarah The error code is a rotary valve error. "Rotary valve does not stop at the required station after 2 times". My guess is that you have a bad rotary valve sensor board. It could also be the sensor disk. Best Matt Mincer Tech One Biomedical Services 708-383-6040 X 10 www.techoneweb.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jul 10 11:48:21 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Jul 10 11:49:10 2010 Subject: SPAM-LOW: [Histonet] air dry or heat dry In-Reply-To: <4C373E8B0200004D000C9B71@hsc-iagate1.health.unm.edu> References: <4C373E8B0200004D000C9B71@hsc-iagate1.health.unm.edu> Message-ID: <57C63DE36DFC4B4280BB9F6523142E48@prueggihctechlt> I really do not think it is a good idea to heat frozen sections, especially repeatedly. I air dry frozen sections, then store them in slide mailers (holds 5) in plastic bags at -80. Take what ever slide mailers I need and put them in a plastic bag I store in the freezer which has some desiccant in it and seal it. I then take the sealed bag with the slide mailers and desiccant out and leave it on the counter top at rt for 15-30 min. before opening the bag. This allows the slides to come to rt without liquid melt forming on the section. You could do the same thing with say using a 100 slot slide box, a plastic bag big enough to hold a rack, in the freezer take out the slides you need for staining, put them in the rack inside the cold plastic bag with some desiccant, seal it up before removing from the freezer and allow to come to rt temp without opening the bag for 15-30 min. How well your IHC staining is after all the kinds of storage and handling you mentioned depends individually on the target of interest. Some targets are hardy and will be fine, while others will be easily destroyed, you need to choose a handling method that will assure the preservation of the weakest target and then you can be sure they are all preserved. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Thompson Sent: Friday, July 09, 2010 3:22 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] air dry or heat dry Hello, We have an ongoing debate in our lab regarding the relative virtue of heat drying slides vs RT air drying them. Tissues: 4% paraformaldehyde perfused rat brains frozen in OCT blocks Sections: 10 microns on a cryostat (at approx -25 C) Slides: Superfrost Plus The slides are stored in slide boxes at -80 C until staining. When staining the heat dry faction (wanting to avoid icy slides) put their slide boxes straight out of the freezeer into a 50 C oven for 30 minutes before taking out slides to stain. then the box goes back to the freezer until the next round of staining, The air dry group feels that cooking the antigens repeatedly at 50 C is problematic so they take the frosty slides out their slide boxes and return them ASAP to the freezer. The slides to be stained are air dried in the fume hood for about 30 min. A third, middle of the road, person takes her slides out of the cold boxes and then puts the slides in a 60 C oven for 30 minutes. For all three groups then, the slides are given a PAP pen border to prepare for IHC and when the pen solution is dry, 10 minutes in acetone and the remainder of the staining procedure. So my question is: Who is using the best technique? Another hotly debated topic is wether it is advisable to put a drop of PBS on the slide before 'sticking' the section to prevent folds in the sections.. Any opinions are appreciated. Thanks, Jeff _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Jul 10 11:52:28 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 10 11:52:33 2010 Subject: [Histonet] air dry or heat dry In-Reply-To: <4C373E8B0200004D000C9B71@hsc-iagate1.health.unm.edu> Message-ID: <245488.6618.qm@web65711.mail.ac4.yahoo.com> The "rule of thumb" is that you never freeze-thaw-freeze protein containing substance (a tissue section is in this category as well as any protein containing solution). Freezing-thawing-freezing can end denaturing the proteins and your IHC results could be compromised. You can safely take out of the box just the slides you need and let them thaw at room temperature. So, answering your specific question, the second group is doing what I used to do and, consequently, I think it?is the best way (no surprises there!). Ren? J. ? ? --- On Fri, 7/9/10, Jeffrey Thompson wrote: From: Jeffrey Thompson Subject: [Histonet] air dry or heat dry To: histonet@lists.utsouthwestern.edu Date: Friday, July 9, 2010, 5:21 PM Hello, We have an ongoing debate in our lab regarding the relative virtue of heat drying slides vs RT air drying them. Tissues: 4% paraformaldehyde perfused rat brains frozen in OCT blocks Sections: 10 microns on a cryostat (at approx -25 C) Slides: Superfrost Plus The slides are stored in slide boxes at -80 C until staining. When staining the heat dry faction (wanting to avoid icy slides) put their slide boxes straight out of the freezeer into a 50 C oven for 30 minutes before taking out slides to stain. then the box goes back to the freezer until the next round of staining, The air dry group feels that cooking the antigens repeatedly at 50 C is problematic so they take the frosty slides out their slide boxes and return them ASAP to the freezer. The slides to be stained are air dried in the fume hood for about 30 min. A third, middle of the road, person takes her slides out of the cold boxes and then puts the slides in a 60 C oven for 30 minutes. For all three groups then, the slides are given a PAP pen border to prepare for IHC and when the pen solution is dry, 10 minutes in acetone and the remainder of the staining procedure. So my question is: Who is using the best technique? Another hotly debated topic is wether it is advisable to put a drop of PBS on the slide before 'sticking' the section to prevent folds in the sections.. Any opinions are appreciated. Thanks, Jeff??? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Sun Jul 11 09:24:02 2010 From: andreahooper <@t> rocketmail.com (andreahooper@rocketmail.com) Date: Sun Jul 11 09:24:34 2010 Subject: [Histonet] air dry or heat dry In-Reply-To: <4C373E8B0200004D000C9B71@hsc-iagate1.health.unm.edu> References: <4C373E8B0200004D000C9B71@hsc-iagate1.health.unm.edu> Message-ID: <347987503-1278858265-cardhu_decombobulator_blackberry.rim.net-1491540757-@bda665.bisx.prod.on.blackberry> I recommend to just take out the slides you need one at a time. If you are concerned about icy slides take them out rapidly without removing the box from the freezer (ie: do it on the shelf in the -80 deg). Also add some dessicant to the slide box. If its a major concern, use smaller slide boxes, or cut less slides, or individually heat seal each slide or small set of slides into their own plastic envelopes. I cut the OCT slides. Dry for about 4h then put at -80 deg C. After freezing I do not allow the slides to warm at all. They go directly from the slide box in freezer into the PFA (or PBS depending). Great morphology. I do not recommend you heat your sections once, much less the whole box repeatedly. This seems like a bad idea over time for sure. Especially if you are trying to repeat experiments and conditions. Why do you acetone fix when your tissues are already PFA fixed? This seems unnecessary. If I had to choose from your selection the best technique, I say the air dry group in the hood for 30 min is best. Andrea Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Jeffrey Thompson" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Fri, 09 Jul 2010 15:21:47 To: Subject: [Histonet] air dry or heat dry Hello, We have an ongoing debate in our lab regarding the relative virtue of heat drying slides vs RT air drying them. Tissues: 4% paraformaldehyde perfused rat brains frozen in OCT blocks Sections: 10 microns on a cryostat (at approx -25 C) Slides: Superfrost Plus The slides are stored in slide boxes at -80 C until staining. When staining the heat dry faction (wanting to avoid icy slides) put their slide boxes straight out of the freezeer into a 50 C oven for 30 minutes before taking out slides to stain. then the box goes back to the freezer until the next round of staining, The air dry group feels that cooking the antigens repeatedly at 50 C is problematic so they take the frosty slides out their slide boxes and return them ASAP to the freezer. The slides to be stained are air dried in the fume hood for about 30 min. A third, middle of the road, person takes her slides out of the cold boxes and then puts the slides in a 60 C oven for 30 minutes. For all three groups then, the slides are given a PAP pen border to prepare for IHC and when the pen solution is dry, 10 minutes in acetone and the remainder of the staining procedure. So my question is: Who is using the best technique? Another hotly debated topic is wether it is advisable to put a drop of PBS on the slide before 'sticking' the section to prevent folds in the sections.. Any opinions are appreciated. Thanks, Jeff _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Sun Jul 11 10:12:57 2010 From: andreahooper <@t> rocketmail.com (andreahooper@rocketmail.com) Date: Sun Jul 11 10:13:04 2010 Subject: [Histonet] VEGFR2 + VEGFR3 Message-ID: <1874648925-1278861178-cardhu_decombobulator_blackberry.rim.net-168187327-@bda665.bisx.prod.on.blackberry> What is everyone's current favorite anti-VEGFR2 and anti-VEGFR3 antibody for staining human tissue? As usual I am in the market for some new reagents. Much appreciated, Andrea Sent from my Verizon Wireless BlackBerry From jcampbell <@t> vdxpathology.com Mon Jul 12 12:07:30 2010 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Mon Jul 12 12:07:37 2010 Subject: [Histonet] Difference between Protease and Proteinase-K? Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF83CFFD2@VDXSERVER01.vdxpathology.local> Hi All, I would like to try enzyme digestion on my CD31 (for K9 and feline tissue) which I haven't been able to get to work using citrate buffer pH 6.0 or pH 9.0. I have heard recommendations for both Protease and Proteinase-K but, do not know which one I should try out. Any feedback on the differences between the two? Thanks, Jennifer Campbell From mward <@t> wfubmc.edu Mon Jul 12 12:10:27 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Mon Jul 12 12:11:31 2010 Subject: [Histonet] Phospho-Histone 3 (SER10) antibody Message-ID: <61135F0455D33347B5AAE209B903A30433DEBB14@EXCHVS2.medctr.ad.wfubmc.edu> I have been trying without success to get this antibody to work. Is anyone doing this antibody? We have the Leica Bond Max and would like to perform it on this platform. I have tried 2 antibodies from Cell Signaling Technology without any success. Any advice would be greatly appreciated. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 From Kjones <@t> upei.ca Mon Jul 12 12:35:40 2010 From: Kjones <@t> upei.ca (Kathleen Jones) Date: Mon Jul 12 12:35:49 2010 Subject: [Histonet] Difference between Protease and Proteinase-K? In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF83CFFD2@VDXSERVER01.vdxpathology.local> References: <5658CBDB9EAE6545ABE50D2563D81BF83CFFD2@VDXSERVER01.vdxpathology.local> Message-ID: <4C3B283D0200008B00027834@grpwise.novell.upei.ca> Hello Jennifer I'm not sure what platform you are using, but from my experience, I obtain beautiful results for CD31 in canine and feline tissue, but the protocols for each are quite different. For CD31 in canine tissue I use a tris based buffer and for feline I use protease. I know this doesn't answer you question, but thought I would pass along what works for me. Good Luck Kathy Kathleen Jones Research Technician Pathology/Microbiology AVC - UPEI (902)566-0595 >>> "Jennifer Campbell" 7/12/2010 2:07 PM >>> Hi All, I would like to try enzyme digestion on my CD31 (for K9 and feline tissue) which I haven't been able to get to work using citrate buffer pH 6.0 or pH 9.0. I have heard recommendations for both Protease and Proteinase-K but, do not know which one I should try out. Any feedback on the differences between the two? Thanks, Jennifer Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Mon Jul 12 12:58:43 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Mon Jul 12 12:58:57 2010 Subject: [Histonet] Phospho-Histone 3 (SER10) antibody In-Reply-To: <61135F0455D33347B5AAE209B903A30433DEBB14@EXCHVS2.medctr.ad.wfubmc.edu> References: <61135F0455D33347B5AAE209B903A30433DEBB14@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <63EA0607835FBA4689CEA9EA8B482692033ED4D9@usctmx1141.merck.com> Martha, We have good success with Millipore 06-570 using citrate HIER - also with the (pSer28) Histone H3 from Epitomics (#1329). Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Monday, July 12, 2010 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Phospho-Histone 3 (SER10) antibody I have been trying without success to get this antibody to work. Is anyone doing this antibody? We have the Leica Bond Max and would like to perform it on this platform. I have tried 2 antibodies from Cell Signaling Technology without any success. Any advice would be greatly appreciated. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From alisha <@t> ka-recruiting.com Mon Jul 12 14:08:52 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Mon Jul 12 14:07:27 2010 Subject: [Histonet] Great Histotech Job in South Carolina Message-ID: <468896864.1278961732674.JavaMail.cfservice@SL4APP1> Dear Histonet Members, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few histotechnician, histotechnologist, and cytotechnologist openings across the country. Our clients typically assist with relocation expenses. One particular client I am working with is looking for a Histotech for a hospital lab in South Carolina. They are looking for someone with 1+ year experience, an HT(ASCP) or HTL(ASCP) certification and someone who is familiar with histology, IHC, and frozen sections. Below is a list of some of the opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current HT and HTL Opportunities: 1. Histotech - Indianapolis, IN 2. Histotechnologist - Atlanta, GA 3. Histotech - NYC 4. Histology Supervisor - Las Vegas, NV 5. Grossing Tech - NYC 6. Histotech - MA 7. IHC Technologist - MA 8. Histology Supervisor - GA 9. Histotech - Otsego County, NY If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From JefThompson <@t> salud.unm.edu Mon Jul 12 16:10:55 2010 From: JefThompson <@t> salud.unm.edu (Jeffrey Thompson) Date: Mon Jul 12 16:11:01 2010 Subject: [Histonet] RE: air dry vs heat dry heat Message-ID: <4C3B307F0200004D000C9F06@hsc-iagate1.health.unm.edu> Hello, Thanks for all the replies. I find them helpful and it may help to resolve some of our lab 'discussions'. Thanks again, Jeff From Maria.Mejia <@t> ucsf.edu Mon Jul 12 17:55:33 2010 From: Maria.Mejia <@t> ucsf.edu (Mejia, Maria) Date: Mon Jul 12 17:55:39 2010 Subject: [Histonet] air dry or heat dry In-Reply-To: <4C373E8B0200004D000C9B71@hsc-iagate1.health.unm.edu> References: <4C373E8B0200004D000C9B71@hsc-iagate1.health.unm.edu> Message-ID: Hello Jeff, Let's see you are sectioning your (fixed) rat brain on the cryostat at 10ums. I'm assuming they are mounted on standard size superfrost plus slides & since they are fixed sections & even if they were not fixed - after sectioning why not air-dry these sections standing up under a fan for 2 hours or longer and store them in plastic slide boxes that hold either 5, 10, 15, or more slides. Only store enough slides in each box for a particular IHC run. Since I don't know what you are staining for - I don't know if storing fixed cryostat sections at -80C is necessary. This is your protocol. Seal each plastic box with cryo tape & place inside a zip-loc bag - fold the bags to the size of box & hold in place with rubber band. Place boxes inside a labeled 3-inch cryo box & store at -80C. When you are ready to run IHC, just remove only what you need from the -80C freezer & quickly return the rest back inside -80C. Even though these sections are fixed (& for those not fixed), once your remove slide boxes from -80C freezer - I would not open the box. Let them equilibriate at room temp on bench top for about 30 mins before removing from plastic bag & slide box. Then run your IHC protocol. Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF San Francisco, CA 94103 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Thompson [JefThompson@salud.unm.edu] Sent: Friday, July 09, 2010 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] air dry or heat dry Hello, We have an ongoing debate in our lab regarding the relative virtue of heat drying slides vs RT air drying them. Tissues: 4% paraformaldehyde perfused rat brains frozen in OCT blocks Sections: 10 microns on a cryostat (at approx -25 C) Slides: Superfrost Plus The slides are stored in slide boxes at -80 C until staining. When staining the heat dry faction (wanting to avoid icy slides) put their slide boxes straight out of the freezeer into a 50 C oven for 30 minutes before taking out slides to stain. then the box goes back to the freezer until the next round of staining, The air dry group feels that cooking the antigens repeatedly at 50 C is problematic so they take the frosty slides out their slide boxes and return them ASAP to the freezer. The slides to be stained are air dried in the fume hood for about 30 min. A third, middle of the road, person takes her slides out of the cold boxes and then puts the slides in a 60 C oven for 30 minutes. For all three groups then, the slides are given a PAP pen border to prepare for IHC and when the pen solution is dry, 10 minutes in acetone and the remainder of the staining procedure. So my question is: Who is using the best technique? Another hotly debated topic is wether it is advisable to put a drop of PBS on the slide before 'sticking' the section to prevent folds in the sections.. Any opinions are appreciated. Thanks, Jeff _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atunde90 <@t> yahoo.com Mon Jul 12 18:51:03 2010 From: atunde90 <@t> yahoo.com (Ade Tunde) Date: Mon Jul 12 18:51:07 2010 Subject: [Histonet] BOND III VS ULTRA BENCH MARK Message-ID: <61269.48112.qm@web46105.mail.sp1.yahoo.com> I?need help about these immunostainers, Does anybody has idea about the best out of the two? I want to know the ADVANTAGES AND DISADVANTAGES of each.Thanks GUY Tunde Ajibade??BS HTL(ASCP)QIHC Pathology lab Newark Beth Israel Medical Center. Newark NJ 07111 From MAUGER <@t> email.chop.edu Tue Jul 13 07:45:01 2010 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Tue Jul 13 07:47:11 2010 Subject: [Histonet] anti mouse CD163 Message-ID: <443F5B475A9BF647AB962E834884EBAD278D12780B@EX7CCRPW03V1.chop.edu> Hi All, Can anyone reccomend a good CD163 antibody that works in mouse FFPE tissue? Thanks, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From sbaldwin <@t> mhhcc.org Tue Jul 13 08:35:05 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Tue Jul 13 08:38:09 2010 Subject: [Histonet] CORRECT BILLING? Message-ID: HISTONETTERS Another question for CPT codes on NON-GYNS (Pelvic washing and etc) and a cell block do you use 88160 & 88305? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From jcox90 <@t> yahoo.com Tue Jul 13 09:07:11 2010 From: jcox90 <@t> yahoo.com (jcox90@yahoo.com) Date: Tue Jul 13 09:07:15 2010 Subject: [Histonet] Histologist opportunity for Private Dermatology practice in Phoenix AZ Message-ID: <202375.45513.qm@web56804.mail.re3.yahoo.com> We have an immediate opening for a histologist in our Dermatology practice. Full time, excellent pay and benefits, great Doctors, flexible hours. Must be able to work independently with little supervision. Please call Jill at 602-481-1424 for more details or email resume to jcazderm@yahoo.com. Jill Cox HT (ASCP) From DKBoyd <@t> chs.net Tue Jul 13 09:12:54 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Jul 13 09:13:03 2010 Subject: [Histonet] CORRECT BILLING? In-Reply-To: Message-ID: We prepare cytospins and charge 88108 for the cytospin preparation and 88305 for the cell block. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Sara Baldwin/mhhcc.org" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/13/2010 09:38 AM To cc Subject [Histonet] CORRECT BILLING? HISTONETTERS Another question for CPT codes on NON-GYNS (Pelvic washing and etc) and a cell block do you use 88160 & 88305? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From kmerriam2003 <@t> yahoo.com Tue Jul 13 10:40:04 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Jul 13 10:40:10 2010 Subject: [Histonet] off-topic "cryosectioning" as security check word Message-ID: <751686.44717.qm@web50305.mail.re2.yahoo.com> Hi All, I thought you all would get a kick out of this.? I was ordering some tickets on ticketmaster and look at the word that popped up?for the security check - it was "cryosectioning"! I managed to get a screenshot of it; it is attached.? We have infiltrated main-stream culture! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From Jackie.O'Connor <@t> abbott.com Tue Jul 13 13:27:54 2010 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jul 13 13:28:56 2010 Subject: [Histonet] Elgin Community College Histology Program In-Reply-To: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> References: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac.wbe@email04.secureserver.net> Message-ID: Does anyone know who is running this program? I would like to contact them. Thanks! From rosenfeldtek <@t> hotmail.com Tue Jul 13 13:30:33 2010 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Tue Jul 13 13:30:40 2010 Subject: [Histonet] RE: Formalin and Xylene odor containment In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2FA@EXCHMBC2.ad.ah.local> References: , , <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2FA@EXCHMBC2.ad.ah.local> Message-ID: A fume hood is the traditional, legal and effective technology. > From: Janice.Mahoney@alegent.org > To: dellav@musc.edu; jphistology@gmail.com; histonet@lists.utsouthwestern.edu > Date: Fri, 9 Jul 2010 14:14:02 -0500 > Subject: RE: [Histonet] Formalin and Xylene odor containment > CC: > > Great advice Vinnie, At a prior job our safety manager found there to be more problems with ozone than what we were trying to fix. > Jan mahoney > Omaha, NE > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie > Sent: Friday, July 09, 2010 2:09 PM > To: 'Joao Pessoa'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Formalin and Xylene odor containment > > I don't have any expertise in this area however I recall reading about the controversies of ozone generators. You would do well to do a bit of internet research before you take this leap. If your facility has a safety officer I'd definitely determine if there is an institution position on ozone generators. > > Here is one link for information but you shouldn't have difficulty coming up with others. > > http://www.epa.gov/iaq/pubs/ozonegen.html > > > Vinnie Della Speranza > Manager for Anatomic Pathology Services > Medical University of South Carolina > 165 Ashley Avenue Suite 309 > Charleston, South Carolina 29425 > Tel: (843) 792-6353 > Fax: (843) 792-8974 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joao Pessoa > Sent: Friday, July 09, 2010 3:01 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Formalin and Xylene odor containment > > All, > > We have recently been using a new piece of demo equipment to control > formalin, xylene and other odors. The unit is an air purifier from BioZone > Scientific. Our ventilation is good in all areas of our lab and we have > always been found to be well below the particulate count allotment for > xylene, formalin, and other compounds. However, many area of the lab still > had a heavy chemical smell (like a beauty salon, they say). This smell goes > away with the BioZone unit, which uses UV light and ozone to break down the > organic compounds. Does anyone else has experience with BioZone? We are > thinking about buying it, but I wanted to hear the opinion of others. > > Cheers, > > Joao > > Histo Tech > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From AWeiss <@t> shorememorial.org Tue Jul 13 14:02:09 2010 From: AWeiss <@t> shorememorial.org (AWeiss@shorememorial.org) Date: Tue Jul 13 14:04:35 2010 Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 14 In-Reply-To: <20100713170225.819BFCFCEF2@smhex1.shorememorial.org> Message-ID: I think Sue is upset about sending the e-mail to more than one person. I sent it to u and to her, do u know what she is talking about? Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org histonet-request@ lists.utsouthwest ern.edu To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject Histonet Digest, Vol 80, Issue 14 07/13/2010 01:02 PM Please respond to histonet@lists.ut southwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Difference between Protease and Proteinase-K? (Jennifer Campbell) 2. Phospho-Histone 3 (SER10) antibody (Martha Ward) 3. Re: Difference between Protease and Proteinase-K? (Kathleen Jones) 4. RE: Phospho-Histone 3 (SER10) antibody (Connolly, Brett M) 5. Great Histotech Job in South Carolina (Alisha Dynan) 6. RE: air dry vs heat dry heat (Jeffrey Thompson) 7. RE: air dry or heat dry (Mejia, Maria) 8. BOND III VS ULTRA BENCH MARK (Ade Tunde) 9. anti mouse CD163 (Mauger, Joanne) 10. CORRECT BILLING? (Sara Baldwin/mhhcc.org) 11. Histologist opportunity for Private Dermatology practice in Phoenix AZ (jcox90@yahoo.com) 12. Re: CORRECT BILLING? (DKBoyd@chs.net) 13. off-topic "cryosectioning" as security check word (Kim Merriam) ---------------------------------------------------------------------- Message: 1 Date: Mon, 12 Jul 2010 10:07:30 -0700 From: "Jennifer Campbell" Subject: [Histonet] Difference between Protease and Proteinase-K? To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF83CFFD2@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="us-ascii" Hi All, I would like to try enzyme digestion on my CD31 (for K9 and feline tissue) which I haven't been able to get to work using citrate buffer pH 6.0 or pH 9.0. I have heard recommendations for both Protease and Proteinase-K but, do not know which one I should try out. Any feedback on the differences between the two? Thanks, Jennifer Campbell ------------------------------ Message: 2 Date: Mon, 12 Jul 2010 13:10:27 -0400 From: "Martha Ward" Subject: [Histonet] Phospho-Histone 3 (SER10) antibody To: Message-ID: <61135F0455D33347B5AAE209B903A30433DEBB14@EXCHVS2.medctr.ad.wfubmc.edu> Content-Type: text/plain; charset="us-ascii" I have been trying without success to get this antibody to work. Is anyone doing this antibody? We have the Leica Bond Max and would like to perform it on this platform. I have tried 2 antibodies from Cell Signaling Technology without any success. Any advice would be greatly appreciated. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ------------------------------ Message: 3 Date: Mon, 12 Jul 2010 14:35:40 -0300 From: "Kathleen Jones" Subject: Re: [Histonet] Difference between Protease and Proteinase-K? To: , "Jennifer Campbell" Message-ID: <4C3B283D0200008B00027834@grpwise.novell.upei.ca> Content-Type: text/plain; charset=US-ASCII Hello Jennifer I'm not sure what platform you are using, but from my experience, I obtain beautiful results for CD31 in canine and feline tissue, but the protocols for each are quite different. For CD31 in canine tissue I use a tris based buffer and for feline I use protease. I know this doesn't answer you question, but thought I would pass along what works for me. Good Luck Kathy Kathleen Jones Research Technician Pathology/Microbiology AVC - UPEI (902)566-0595 >>> "Jennifer Campbell" 7/12/2010 2:07 PM >>> Hi All, I would like to try enzyme digestion on my CD31 (for K9 and feline tissue) which I haven't been able to get to work using citrate buffer pH 6.0 or pH 9.0. I have heard recommendations for both Protease and Proteinase-K but, do not know which one I should try out. Any feedback on the differences between the two? Thanks, Jennifer Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 12 Jul 2010 13:58:43 -0400 From: "Connolly, Brett M" Subject: RE: [Histonet] Phospho-Histone 3 (SER10) antibody To: "Martha Ward" , Message-ID: <63EA0607835FBA4689CEA9EA8B482692033ED4D9@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" Martha, We have good success with Millipore 06-570 using citrate HIER - also with the (pSer28) Histone H3 from Epitomics (#1329). Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Monday, July 12, 2010 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Phospho-Histone 3 (SER10) antibody I have been trying without success to get this antibody to work. Is anyone doing this antibody? We have the Leica Bond Max and would like to perform it on this platform. I have tried 2 antibodies from Cell Signaling Technology without any success. Any advice would be greatly appreciated. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 5 Date: 12 Jul 2010 15:08:52 -0400 From: Alisha Dynan Subject: [Histonet] Great Histotech Job in South Carolina To: histonet@lists.utsouthwestern.edu Message-ID: <468896864.1278961732674.JavaMail.cfservice@SL4APP1> Content-Type: text/plain; charset="utf-8" Dear Histonet Members, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few histotechnician, histotechnologist, and cytotechnologist openings across the country. Our clients typically assist with relocation expenses. One particular client I am working with is looking for a Histotech for a hospital lab in South Carolina. They are looking for someone with 1+ year experience, an HT(ASCP) or HTL(ASCP) certification and someone who is familiar with histology, IHC, and frozen sections. Below is a list of some of the opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current HT and HTL Opportunities: 1. Histotech - Indianapolis, IN 2. Histotechnologist - Atlanta, GA 3. Histotech - NYC 4. Histology Supervisor - Las Vegas, NV 5. Grossing Tech - NYC 6. Histotech - MA 7. IHC Technologist - MA 8. Histology Supervisor - GA 9. Histotech - Otsego County, NY If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 6 Date: Mon, 12 Jul 2010 15:10:55 -0600 From: "Jeffrey Thompson" Subject: [Histonet] RE: air dry vs heat dry heat To: Message-ID: <4C3B307F0200004D000C9F06@hsc-iagate1.health.unm.edu> Content-Type: text/plain; charset=US-ASCII Hello, Thanks for all the replies. I find them helpful and it may help to resolve some of our lab 'discussions'. Thanks again, Jeff ------------------------------ Message: 7 Date: Mon, 12 Jul 2010 15:55:33 -0700 From: "Mejia, Maria" Subject: RE: [Histonet] air dry or heat dry To: Jeffrey Thompson , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Jeff, Let's see you are sectioning your (fixed) rat brain on the cryostat at 10ums. I'm assuming they are mounted on standard size superfrost plus slides & since they are fixed sections & even if they were not fixed - after sectioning why not air-dry these sections standing up under a fan for 2 hours or longer and store them in plastic slide boxes that hold either 5, 10, 15, or more slides. Only store enough slides in each box for a particular IHC run. Since I don't know what you are staining for - I don't know if storing fixed cryostat sections at -80C is necessary. This is your protocol. Seal each plastic box with cryo tape & place inside a zip-loc bag - fold the bags to the size of box & hold in place with rubber band. Place boxes inside a labeled 3-inch cryo box & store at -80C. When you are ready to run IHC, just remove only what you need from the -80C freezer & quickly return the rest back inside -80C. Even though these sections are fixed (& for those not fixed), once your remove slide boxes from -80C freezer - I would not open the box. Let them equilibriate at room temp on bench top for about 30 mins before removing from plastic bag & slide box. Then run your IHC protocol. Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF San Francisco, CA 94103 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Thompson [JefThompson@salud.unm.edu] Sent: Friday, July 09, 2010 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] air dry or heat dry Hello, We have an ongoing debate in our lab regarding the relative virtue of heat drying slides vs RT air drying them. Tissues: 4% paraformaldehyde perfused rat brains frozen in OCT blocks Sections: 10 microns on a cryostat (at approx -25 C) Slides: Superfrost Plus The slides are stored in slide boxes at -80 C until staining. When staining the heat dry faction (wanting to avoid icy slides) put their slide boxes straight out of the freezeer into a 50 C oven for 30 minutes before taking out slides to stain. then the box goes back to the freezer until the next round of staining, The air dry group feels that cooking the antigens repeatedly at 50 C is problematic so they take the frosty slides out their slide boxes and return them ASAP to the freezer. The slides to be stained are air dried in the fume hood for about 30 min. A third, middle of the road, person takes her slides out of the cold boxes and then puts the slides in a 60 C oven for 30 minutes. For all three groups then, the slides are given a PAP pen border to prepare for IHC and when the pen solution is dry, 10 minutes in acetone and the remainder of the staining procedure. So my question is: Who is using the best technique? Another hotly debated topic is wether it is advisable to put a drop of PBS on the slide before 'sticking' the section to prevent folds in the sections.. Any opinions are appreciated. Thanks, Jeff _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 12 Jul 2010 16:51:03 -0700 (PDT) From: Ade Tunde Subject: [Histonet] BOND III VS ULTRA BENCH MARK To: histonet@lists.utsouthwestern.edu Message-ID: <61269.48112.qm@web46105.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I?need help about these immunostainers, Does anybody has idea about the best out of the two? I want to know the ADVANTAGES AND DISADVANTAGES of each.Thanks GUY Tunde Ajibade??BS HTL(ASCP)QIHC Pathology lab Newark Beth Israel Medical Center. Newark NJ 07111 ------------------------------ Message: 9 Date: Tue, 13 Jul 2010 08:45:01 -0400 From: "Mauger, Joanne" Subject: [Histonet] anti mouse CD163 To: "histonet@lists.utsouthwestern.edu" Message-ID: <443F5B475A9BF647AB962E834884EBAD278D12780B@EX7CCRPW03V1.chop.edu> Content-Type: text/plain; charset="us-ascii" Hi All, Can anyone reccomend a good CD163 antibody that works in mouse FFPE tissue? Thanks, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ ------------------------------ Message: 10 Date: Tue, 13 Jul 2010 09:35:05 -0400 From: "Sara Baldwin/mhhcc.org" Subject: [Histonet] CORRECT BILLING? To: Message-ID: Content-Type: text/plain; charset=ISO-8859-1 HISTONETTERS Another question for CPT codes on NON-GYNS (Pelvic washing and etc) and a cell block do you use 88160 & 88305? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ------------------------------ Message: 11 Date: Tue, 13 Jul 2010 07:07:11 -0700 (PDT) From: jcox90@yahoo.com Subject: [Histonet] Histologist opportunity for Private Dermatology practice in Phoenix AZ To: "Histonet@Lists. Utsouthwestern. Edu" Message-ID: <202375.45513.qm@web56804.mail.re3.yahoo.com> Content-Type: text/plain; charset=us-ascii We have an immediate opening for a histologist in our Dermatology practice. Full time, excellent pay and benefits, great Doctors, flexible hours. Must be able to work independently with little supervision. Please call Jill at 602-481-1424 for more details or email resume to jcazderm@yahoo.com. Jill Cox HT (ASCP) ------------------------------ Message: 12 Date: Tue, 13 Jul 2010 10:12:54 -0400 From: DKBoyd@chs.net Subject: Re: [Histonet] CORRECT BILLING? To: "Sara Baldwin/mhhcc.org" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We prepare cytospins and charge 88108 for the cytospin preparation and 88305 for the cell block. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Sara Baldwin/mhhcc.org" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/13/2010 09:38 AM To cc Subject [Histonet] CORRECT BILLING? HISTONETTERS Another question for CPT codes on NON-GYNS (Pelvic washing and etc) and a cell block do you use 88160 & 88305? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 13 Date: Tue, 13 Jul 2010 08:40:04 -0700 (PDT) From: Kim Merriam Subject: [Histonet] off-topic "cryosectioning" as security check word To: Histonet Message-ID: <751686.44717.qm@web50305.mail.re2.yahoo.com> Content-Type: text/plain; charset="iso-8859-1" Hi All, I thought you all would get a kick out of this.? I was ordering some tickets on ticketmaster and look at the word that popped up?for the security check - it was "cryosectioning"! I managed to get a screenshot of it; it is attached.? We have infiltrated main-stream culture! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 14 **************************************** From Lynn.Burton <@t> Illinois.gov Tue Jul 13 14:19:58 2010 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Tue Jul 13 14:20:31 2010 Subject: [Histonet] FW: M. Bovis IHC question References: Message-ID: ________________________________ From: Burton, Lynn Sent: Tue 7/13/2010 2:17 PM To: histonet-bounces@lists.utsouthwestern.edu Subject: M. Bovis IHC question If anyone out there is using M. Bovis antobody from Chemicon can you please tell me what dilution you arer using? We had a computer crash last year and I have lost this information. Thank You. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From lynnlee2010 <@t> live.com Tue Jul 13 20:15:08 2010 From: lynnlee2010 <@t> live.com (Lynn L) Date: Tue Jul 13 20:15:12 2010 Subject: [Histonet] assisting with patient bone marrows Message-ID: Can anyone provide some input about which department (Hematology, Histology etc.) assists on the patient floors with bone marrow aspirations and to what extent? Thanks in advance! Lynn Lee Casa Grande, AZ _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From godsgalnow <@t> aol.com Tue Jul 13 20:19:09 2010 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Jul 13 20:19:15 2010 Subject: [Histonet] assisting with patient bone marrows Message-ID: <938654094-1279070350-cardhu_decombobulator_blackberry.rim.net-1200712598-@bda2106.bisx.prod.on.blackberry> I have worked in places that use techs from hematology and I've worked at places that use histotechs. So it depends on the institution and any regulations set forth by your state, if any. Roxanne ------Original Message------ From: Lynn L Sender: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] assisting with patient bone marrows Sent: Jul 13, 2010 9:15 PM Can anyone provide some input about which department (Hematology, Histology etc.) assists on the patient floors with bone marrow aspirations and to what extent? Thanks in advance! Lynn Lee Casa Grande, AZ _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent from my Verizon Wireless BlackBerry From carl.hobbs <@t> kcl.ac.uk Wed Jul 14 03:46:58 2010 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Jul 14 03:47:25 2010 Subject: [Histonet] Phospho-Histone 3 (SER10) antibody Message-ID: <11D9615B89C10747B1C985966A63D7CA2D7BE53D40@KCL-MAIL04.kclad.ds.kcl.ac.uk> I use Upstate's 06-570. Excellent for human, rat, mouse FFPW sections after HIER. I have no idea what amino acid is phosphorylated but, if you are wanting an Ab against cells in mitosis only, this is the best, imho. Carl Carl Hobbs Histology Manager Wolfson Centre for Age-Related Diseases King's College London Tel.020 7848 6810 From DKBoyd <@t> chs.net Wed Jul 14 06:43:25 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Jul 14 06:43:32 2010 Subject: [Histonet] assisting with patient bone marrows In-Reply-To: Message-ID: Back in the 80-late 90's Hematology assisted. Since the late 90's Histology assist with all bone marrows. We have a Wescor Wright stainer and we stain all smears for the bone marrow including the peripheral smear. It all started with Hema not being able to perform the Fe stain on smears to the pathologist liking. First we got the Fe stain then it wasn't long we got the whole shebang! Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Lynn L Sent by: histonet-bounces@lists.utsouthwestern.edu 07/13/2010 09:16 PM To cc Subject [Histonet] assisting with patient bone marrows Can anyone provide some input about which department (Hematology, Histology etc.) assists on the patient floors with bone marrow aspirations and to what extent? Thanks in advance! Lynn Lee Casa Grande, AZ _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Wanda.Smith <@t> HCAhealthcare.com Wed Jul 14 07:28:31 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Jul 14 07:28:39 2010 Subject: [Histonet] assisting with patient bone marrows In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA138F29B469@NADCWPMSGCMS03.hca.corpad.net> Good Morning, At this point, our Hematology Dept assists on BMs on the floors. If they could, they would pass it on to us, but we do not have the staff (or desire) to do it. My past 2 HT hires came from places where the histotechs assisted on the BM collection. Hope that helps! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynn L Sent: Tuesday, July 13, 2010 9:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] assisting with patient bone marrows Can anyone provide some input about which department (Hematology, Histology etc.) assists on the patient floors with bone marrow aspirations and to what extent? Thanks in advance! Lynn Lee Casa Grande, AZ _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Jul 14 07:42:52 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Jul 14 07:42:56 2010 Subject: [Histonet] tungsten knives for cryostat Message-ID: <76206.22355.qm@web50304.mail.re2.yahoo.com> Hi All, I am in need of some disposable tunsten knives for my cryostat.? It currently has a low-profile blade holder on it, but I can't seem to find any disposable tungsten knives that are low-profile.? Does anyone know if they are available? If not, I can just buy some high-profile ones and get a new?adaptor for my knife holder.? What company makes the best tungsten knives? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From brett_connolly <@t> merck.com Wed Jul 14 08:21:16 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Jul 14 08:21:20 2010 Subject: [Histonet] Phospho-Histone 3 (SER10) antibody In-Reply-To: <11D9615B89C10747B1C985966A63D7CA2D7BE53D40@KCL-MAIL04.kclad.ds.kcl.ac.uk> References: <11D9615B89C10747B1C985966A63D7CA2D7BE53D40@KCL-MAIL04.kclad.ds.kcl.ac.uk> Message-ID: <63EA0607835FBA4689CEA9EA8B4826920342FC2C@usctmx1141.merck.com> Carl et al. Upstate is now part of Millipore. The 06-570 antibody is anti-Histone H3 (pSer10), the same one I referenced the other day. Best, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl Sent: Wednesday, July 14, 2010 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Phospho-Histone 3 (SER10) antibody I use Upstate's 06-570. Excellent for human, rat, mouse FFPW sections after HIER. I have no idea what amino acid is phosphorylated but, if you are wanting an Ab against cells in mitosis only, this is the best, imho. Carl Carl Hobbs Histology Manager Wolfson Centre for Age-Related Diseases King's College London Tel.020 7848 6810 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From MW <@t> PersonifySearch.com Wed Jul 14 08:24:27 2010 From: MW <@t> PersonifySearch.com (Matthew Ward) Date: Wed Jul 14 08:24:31 2010 Subject: [Histonet] Seeking Histotechs for Positions with a World Leader in Cancer Diagnostics! Message-ID: <002401cb2357$e277a590$a766f0b0$@com> Our team here at Personify has recently been partnered with a World Leader in Cancer Diagnostics who is going through a large expansion due to their recent and continued success in the Immunohistochemistry market. Due to this expansion we are currently seeking Histotechs who have experience working with IHC analyzers that would be interested in Field Support roles. These Positions Offer: - Outstanding Base Salary and Bonus! - Gold Standard Benefits including but not limited to Medical, Cell Phone, Laptop, Car Allowance, Expenses, 401k, Paid Vacation! - Opportunity for Career Advancement! We currently have positions open in the following areas and will have more locations throughout the year! Southeast Florida (Miami/ FT. Lauderdale) Canada CO/WY/MT MD/VA/DC NM/TX/West OK NYC/Bronx/NJ So. IL/Iowa TN/MS/AR If you are interested in learning more please contact me directly at 800.875.6188 ext. 103 or mw@personifysearch.com Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From mward <@t> wfubmc.edu Wed Jul 14 08:29:04 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Wed Jul 14 08:39:31 2010 Subject: [Histonet] Phospho-Histone 3 (SER10) antibody In-Reply-To: <63EA0607835FBA4689CEA9EA8B4826920342FC2C@usctmx1141.merck.com> References: <11D9615B89C10747B1C985966A63D7CA2D7BE53D40@KCL-MAIL04.kclad.ds.kcl.ac.uk> <63EA0607835FBA4689CEA9EA8B4826920342FC2C@usctmx1141.merck.com> Message-ID: <61135F0455D33347B5AAE209B903A30433DEBB2F@EXCHVS2.medctr.ad.wfubmc.edu> Thank you to everyone who responded to this question. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Wednesday, July 14, 2010 9:21 AM To: Hobbs, Carl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Phospho-Histone 3 (SER10) antibody Carl et al. Upstate is now part of Millipore. The 06-570 antibody is anti-Histone H3 (pSer10), the same one I referenced the other day. Best, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl Sent: Wednesday, July 14, 2010 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Phospho-Histone 3 (SER10) antibody I use Upstate's 06-570. Excellent for human, rat, mouse FFPW sections after HIER. I have no idea what amino acid is phosphorylated but, if you are wanting an Ab against cells in mitosis only, this is the best, imho. Carl Carl Hobbs Histology Manager Wolfson Centre for Age-Related Diseases King's College London Tel.020 7848 6810 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Wed Jul 14 09:12:23 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Jul 14 09:12:30 2010 Subject: [Histonet] Senior IHC Candidate Needed Message-ID: Allied Search Partners is currently holding a confidential search, at the request of our client, for a Senior IHC Technologist. The position is centrally located just east of NYC. *Position Title:* Senior Immunohistochemistry (IHC) Technologist *Requirements:* ? Oncology experience. ? HT or HTL certification ? QIHC preferred but not required ? Understanding of Molecular Pathology and Molecular Diagnostics ? Degree required ? Bone Marrow Experience *Other Requirements:* ? Passionate about personalized medicine ? Prefer about 5-10 years experience (Will consider a candidate near those requirements depending on other factors of their background). *Extras:* Relocation Package Offered *Benefits: * ? Competitive salary commensurate with experience ? 3 Weeks Paid Vacation ? Excellent Health Benefits ? Participation in 401K and Flexible Spending Account Plan ? Incentive driven Stock Option Plan * * *Application:* At our clients request this listing is confidential. To be considered please submit your resume in order to schedule a phone screen with one of our recruiters. Please be aware that all resumes submitted to Allied Search Partners are confidential. *Serious inquiries only please.* 1. No resume will be submitted until we speak to you for a phone screen. 2. No resume will be submitted to client if you are currently or previously worked for client. 3. Name, and contact information will not be given out without your consent. Please submit resume to Alyssa@alliedsearchpartners.com -- Alyssa Peterson, Director of Candidate Recruitment Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From relia1 <@t> earthlink.net Wed Jul 14 09:38:58 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jul 14 09:39:04 2010 Subject: [Histonet] RELIA Histology Job Alert 7-14-2010 Histotechnologist needed for a brand new state of the art lab in Orlando, FL and other great opportunities Message-ID: Hi Histonetters! I hope everyone is keeping cool during these hot summer days. I have a few new positions I want to tell you about. All of the opportunities that I work with are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance. Here are my newest opportunities: Histotechnologist - Night Shift Orlando, FL - State of the Art brand new lab (Forida technologist license req) Histology Manager - Kansas City, MO National reference lab I also have these exciting opportunities: Histotechnician/Histotechnologist - Cape Cod, MA Immunohistochemistry Tech - Night Shift Dallas, TX Histotechnician/Histotechnologist - Night Shift, Austin, TX Histotechnician/Histotechnologist - Night Shift - Boston, MA Histotechnician/Histotechnologist - Evening Shift Suffern, NY NYS license required. For more information on any of these positions or to start a job search customized to a specific area you are interested in please contact me. I offer over 25 years experience helping people find the right position at the right time. All inquiries are confidential and your job search will be discreet and private with me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. Remember all it takes is being in the right place at the right time for the right opportunity and I can help you make that happen! Have a great day. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From jclark <@t> pcnm.com Wed Jul 14 10:06:03 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Jul 14 10:06:07 2010 Subject: [Histonet] Microtome Aligner Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C012D809F@mail.pcnm.com> Hi All, I just want to clarify that the microtome aligner that we are having issues with is the one made by Advanced Innovations and sold by Newcomer Supply not the one made by Tech One (Shane Perkins) which Newcomer also sells. I have had no experience with the device that Shane is referring to. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico From sgoebel <@t> xbiotech.com Wed Jul 14 10:44:40 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Jul 14 10:44:46 2010 Subject: [Histonet] Ahhhh!!! Message-ID: <20100714084440.9e2d9aa830e8449a2412eb1e4f2f067e.df7cfc6f76.wbe@email04.secureserver.net> So I am staining frozen tissue microarrays with an antibody that supposed to bind to monocytes. My problem is in some tissues this is working, but in others it is not. So...dilute out the antibody mor where it sh want it to, but ove is working on cyt ideas? It is (the cells 95% a from cytology to IHC).&n stuck!! Thanks! Sarah Goebel, B.A., HT (ASCP) Histotechnician 8201 East Riversid Austin, Texas (512)386-5107 From Rcartun <@t> harthosp.org Wed Jul 14 10:46:44 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jul 14 10:46:53 2010 Subject: [Histonet] Technical charge - 88342 Message-ID: <4C3DA3A3.7400.0077.1@harthosp.org> I was just informed that our technical charge (Part A) for 88342 (Immunohistochemistry) is over $140. That seems high to me. What do you think? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From mtitford <@t> aol.com Wed Jul 14 11:04:36 2010 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Jul 14 11:04:54 2010 Subject: [Histonet] Technical charge 88342 Message-ID: <8CCF185640AC62E-1284-6848@webmail-m035.sysops.aol.com> Dr Cartun sent an email to the Histonet about what his institution bills for the technical component of 88342. Dr Cartun - Don't feel so guilty! In reality no one much pays top dollar for any service in the hospital. Some patients are treated as DRG patients (Hospital gets paid set amount for treating the patient) and for other patients, their insurence company has contracts with the hospital, where the insurence company (BC/BS for example) pays what they think your cost is. If your are still curious, contact your billing office and ask what Medicare pays for a 88342. Sadly, the only people who pay top dollar are often those without insurence. Michael Titford Pathology USA Mobile AL From Kim.Donadio <@t> bhcpns.org Wed Jul 14 11:08:52 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Jul 14 11:09:07 2010 Subject: [Histonet] Technical charge - 88342 In-Reply-To: <4C3DA3A3.7400.0077.1@harthosp.org> Message-ID: Not to me. Anyway, just because that's what you charge don't mean that's what you're going to get in reimbursement. Medicare and Medicaid have a set fee and it's low. And most insurance only pay a percentage of what you charge, that varies with the company and the deal they make with your hospital. If you are trying to do a cost analysis for bringing something in house I don't recommend using what you charge, You could end up in debt. Just my two cents worth though. Hope this helps :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Richard Cartun" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/14/2010 10:46 AM To "Histonet" cc Subject [Histonet] Technical charge - 88342 I was just informed that our technical charge (Part A) for 88342 (Immunohistochemistry) is over $140. That seems high to me. What do you think? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From BradyM <@t> uthscsa.edu Wed Jul 14 11:15:33 2010 From: BradyM <@t> uthscsa.edu (Brady, Michelle) Date: Wed Jul 14 11:15:39 2010 Subject: [Histonet] Unsubscribe Message-ID: <2915B61D2F093543AE0ECB3E11F1409F40D915F3DD@HSC-M2.win.uthscsa.edu> Unsubscribe Michelle M. Brady Technical Director, Histology Laboratory Greehey Children's Cancer Research Institute University of Texas Health Science Center Mail code 7784 8403 Floyd Curl Drive San Antonio, TX 78229-3900 Office 210-562-9141 Fax 210-562-9135 Email bradym@uthscsa.edu From svobach <@t> kmcpa.com Wed Jul 14 11:34:55 2010 From: svobach <@t> kmcpa.com (Stacy Vobach) Date: Wed Jul 14 11:35:03 2010 Subject: [Histonet] unsubscribe Message-ID: <00c801cb2372$7e9f3cc0$7bddb640$@com> Stacy L. Vobach Human Resource Manager Kansas Medical Clinic P.A. 2200 SW 6th Street Topeka, KS 66606 785-354-1040 x329 From dellav <@t> musc.edu Wed Jul 14 12:42:58 2010 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Jul 14 12:43:07 2010 Subject: [Histonet] New online Histology study program !! In-Reply-To: <2915B61D2F093543AE0ECB3E11F1409F40D915F3DD@HSC-M2.win.uthscsa.edu> References: <2915B61D2F093543AE0ECB3E11F1409F40D915F3DD@HSC-M2.win.uthscsa.edu> Message-ID: Greetings everyone, Occasionally I see posts from individuals seeking advice on where they can get the instruction they need to become a certified histotech. It is for this reason that I am sharing this information. The Histotechnology School at MUSC is beginning an online instructional program for students who would not find it feasible to come to Charleston to study with us directly. Our school began in 2004 and is NAACLS accredited. The online study option is a new option of our program. Our school's website can be accessed at www.musc.edu/histoprogram I should point out that our program is for individuals seeking to become histotechnologist (HTL) certified. Applicants must have their baccalaureate degree in order to be eligible for enrollment. Below I am providing information I received from our school's program director, Karen Geils, and ask that you share it with anyone who might benefit. I've also included Karen's contact information so that she can be reached for further information. I hope that you will contact Karen if our program can be of any help to you. All didactic material will be delivered online via asynchronous media, and clinical experience will be provided by the affiliate laboratory. The course management system, WebCT will be used to deliver assignments, lecture material, and tests. Students will be required to have access to a personal computer with internet. Minimum hardware standards (Mac or PC): PC Software Mac Software CPU with minimum of 2.0GHz 1 GB RAM (2 GB if using Windows Vista) 80GB Hard Drive 2 USB 2.0 ports IEE 1394 "Firewire" CD/DVD RW +/- Drive Sound Card (external or internal speakers) Microphone headset Windows XP, Vista, or 7 Microsoft Office Adobe Reader Internet Browser - IE or Firefox Mac OS X version 10.4 or 10.5 Microsoft Office for Mac Adobe Reader Internet Browser - Firefox The fee is $3600.00 and includes the cost of textbooks. Clinical sites are responsible for laboratory supplies and equipment. MUSC will verify that the clinical experience is comparable to that of the MUSC onsite program. Karen Brinker Geils, MS, HT(ASCP), CT(ASCP) Director, Histotechnology Program Department of Pathology and Laboratory Medicine Medical University of South Carolina 843-792-4013 brinkerk@musc.edu Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 From MAUGER <@t> email.chop.edu Wed Jul 14 14:21:51 2010 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Wed Jul 14 14:25:18 2010 Subject: [Histonet] Mouse macrophage marker Message-ID: <443F5B475A9BF647AB962E834884EBAD278D127811@EX7CCRPW03V1.chop.edu> Hi everyone, I need help finding a macrophage marker that works on FFPE mouse tissue. It can not stain myeloid cells! Please share if you know a good one. Thanks, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From RCazares <@t> schosp.org Wed Jul 14 15:00:34 2010 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Wed Jul 14 15:00:40 2010 Subject: [SPAM-HC] - [Histonet] Microtome Aligner - Email found in subject In-Reply-To: <0CDA5E1E01301F4880A8A7A8BCBDA39C012D809F@mail.pcnm.com> References: <0CDA5E1E01301F4880A8A7A8BCBDA39C012D809F@mail.pcnm.com> Message-ID: <572D1F45B44B3D4096D554B4CB40639C031FF7F104@EXCHCCRMB.schosp.org> Hello Histonetters, I have just received the microtome aligner tool from TechOne that Newcomer has. I have used it on my three microtomes and let me tell you guys, I LOVE IT!! It is very easy to use, just follow the instructions that come with it. I strongly recommend it (but that's just my opinion). Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Wednesday, July 14, 2010 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] Microtome Aligner - Email found in subject Hi All, I just want to clarify that the microtome aligner that we are having issues with is the one made by Advanced Innovations and sold by Newcomer Supply not the one made by Tech One (Shane Perkins) which Newcomer also sells. I have had no experience with the device that Shane is referring to. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From Rick.Garnhart <@t> memorialhealthsystem.com Wed Jul 14 15:09:00 2010 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Wed Jul 14 15:09:04 2010 Subject: [Histonet] Excluded and Limited Tissues to Histo In-Reply-To: Message-ID: Does anyone know if CAP Today has an article within the last couple of years as to what tissue can be excluded from submission and for gross only exams. The last article I have reference to was from 1996. Thanks Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From Keri.Colwell <@t> inspection.gc.ca Wed Jul 14 15:52:53 2010 From: Keri.Colwell <@t> inspection.gc.ca (Keri Colwell) Date: Wed Jul 14 15:52:59 2010 Subject: [Histonet] Waste disposal Message-ID: <4C3DCF44.C0AD.00BF.0@inspection.gc.ca> Hi, I am currently working on ways to enhance my DAB stiaing and find I am generating two types of waste that I don't know how to deal with. One of my techniques involves post-DAB enhancement in a cupric sulfate solution. My other technique involves making my DAB solution and including some nickel chloride. Does anyone know how I should be disposing of the cupric sulfate solution, or the DAB containing nickel chloride? Thanks! Keri From vavalos <@t> allergydermatology.com Wed Jul 14 18:28:34 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Wed Jul 14 18:28:47 2010 Subject: [Histonet] Autostainer Input Message-ID: <000e01cb23ac$4d0e9720$e72bc560$@com> Our Linear stainer is at its last days and we are looking at purchasing an Autostainer. The two that I have been asked to look at are the Leica Autostainer XL & Sakura DRS 2000. Does anyone have any input on either of the two? Good & Bad will be appreciated!! V.Avalos ADS, INC Fax:602-277-2134 From annigyg <@t> gmail.com Wed Jul 14 20:26:45 2010 From: annigyg <@t> gmail.com (annigyg@gmail.com) Date: Wed Jul 14 20:27:35 2010 Subject: [Histonet] Autostainer Input In-Reply-To: <000e01cb23ac$4d0e9720$e72bc560$@com> References: <000e01cb23ac$4d0e9720$e72bc560$@com> Message-ID: <2096783032-1279157248-cardhu_decombobulator_blackberry.rim.net-383602878-@bda242.bisx.produk.on.blackberry> Sakura DRS is a true asset to any lab. Easy to program and a breeze to use. Almost maintenance free. Have been using DRS's for almost 15years and have yet to find anything better. Just my 2cents worth AnnieinArabia Empower your Business with BlackBerry? and Mobile Solutions from Etisalat -----Original Message----- From: "Vanessa Avalos" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 14 Jul 2010 16:28:34 To: Subject: [Histonet] Autostainer Input Our Linear stainer is at its last days and we are looking at purchasing an Autostainer. The two that I have been asked to look at are the Leica Autostainer XL & Sakura DRS 2000. Does anyone have any input on either of the two? Good & Bad will be appreciated!! V.Avalos ADS, INC Fax:602-277-2134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Thu Jul 15 02:18:20 2010 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Thu Jul 15 02:18:27 2010 Subject: [Histonet] assisting with patient bone marrows In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA138F29B469@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA138F29B469@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AF9DCB391@FWDCWPMSGCMS09.hca.corpad.net> Everyplace I have worked it was done by hematology. When hemo tried to dump this job on us the pathologist stepped in to remind them that this is their area of expertise. We do not expect hemo to do frozen sections and we do not do bone marrows. After they have made their smears and sent the flow they drop off the core and asp. and one smear for iron to us. We handle decaling the bone. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Wednesday, July 14, 2010 8:29 AM To: lynnlee2010@live.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] assisting with patient bone marrows Good Morning, At this point, our Hematology Dept assists on BMs on the floors. If they could, they would pass it on to us, but we do not have the staff (or desire) to do it. My past 2 HT hires came from places where the histotechs assisted on the BM collection. Hope that helps! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynn L Sent: Tuesday, July 13, 2010 9:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] assisting with patient bone marrows Can anyone provide some input about which department (Hematology, Histology etc.) assists on the patient floors with bone marrow aspirations and to what extent? Thanks in advance! Lynn Lee Casa Grande, AZ _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From V.Tilston <@t> liverpool.ac.uk Thu Jul 15 03:09:45 2010 From: V.Tilston <@t> liverpool.ac.uk (Tilston, Valerie) Date: Thu Jul 15 03:10:11 2010 Subject: [Histonet] Resin embedded tissue Message-ID: Hi, Does anyone have experience of cutting resin embedded bone and if so what type of slides do people use? We are having problems with sections falling off or the bone not remaining flat on the slide! Many thanks in advance, Val From ratliffjack <@t> hotmail.com Thu Jul 15 07:17:59 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Jul 15 07:18:13 2010 Subject: [Histonet] Resin embedded tissue In-Reply-To: References: Message-ID: Valerie, I use just regular glass slides, coat them with Haupt's adhesive, roll the sections flat and then press and dry the sections to the coated slide using a slide press and oven at 50C. In 13 years of hard tissue resin histology, I have never (knocking twice on wood) lost a section or had a section lift up in any area during staining using this method. You will get a degree of background with certain stains (hematoxylin, analine blue, etc) because it is a gelatin based adhesive, but these problems are easily resolved with a little overstaining and acid alcohol rinse. If interested you can get the Haupt's and slide press from Dorn and Hart Microedge (www.dornandhart.com). I have also been told that they have a couple of new kits coming out by the end of the summer - a resin embedding kit (using Perkadox as a catalyst) and thin section microtomy kit (one for a rotary microtome and one for a sledge or Polycut microtome) that includes everything needed to section resin blocks. Look them up and contact Bill Hart for more information. Jack On Jul 15, 2010, at 3:09 AM, "Tilston, Valerie" wrote: > Hi, > > Does anyone have experience of cutting resin embedded bone and if so what type of slides do people use? We are having problems with sections falling off or the bone not remaining flat on the slide! > > Many thanks in advance, > > Val > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From annigyg <@t> gmail.com Thu Jul 15 07:25:38 2010 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Jul 15 07:25:44 2010 Subject: [Histonet] assisting with patient bone marrows In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2AF9DCB391@FWDCWPMSGCMS09.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA138F29B469@NADCWPMSGCMS03.hca.corpad.net> <4BF03F5404EBDE409AF9232DA74B9DED2AF9DCB391@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: Oh my - how this makes me smile poor Hema are SUCH busy ones and we in Histo sit around just longing to be able to participate in the 'real' action clinical stuff we are just the dumping ground for all the crap (no disrespect intended to patient samples) that 'others' dont feel like doing. We even get Semen analysis in the Cytology section because at that time the head of Hema refused all body fluids. Now they are doing fluids but Histo is still stuck with the Semens As for the rest of their work, we do the trehines and the specials on the aspirate smears but they do all their own collections. Hema was my first field of speciality so i know what they do - but they have no clue what I do. we print labels for them, help them when their regents run out or expire, supply solvents so they can get their oily mess off the scope objectives - they are not allowed to use the multiheader because of objective 'contamination' when it came to getting their Flow Cytometry built out in Cerner, again we stepped in and did the whole lot. our lab amanger used to be the lead tech in Hema so they are very dear to him - budget and staffing always swings their way. Histo gets the crumbs after all the other sections take their slice - LOL But...I have the best team in the building thats my end-of-week-rant and i feel better for sharing my weekend starts here have a happy friday and enjoy your weekend AnnieinArabia On 15 July 2010 11:18, wrote: > Everyplace I have worked it was done by hematology. When hemo tried to dump > this job on us the pathologist stepped in to remind them that this is their > area of expertise. We do not expect hemo to do frozen sections and we do not > do bone marrows. After they have made their smears and sent the flow they > drop off the core and asp. and one smear for iron to us. We handle decaling > the bone. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Wanda.Smith@HCAhealthcare.com > Sent: Wednesday, July 14, 2010 8:29 AM > To: lynnlee2010@live.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] assisting with patient bone marrows > > Good Morning, > At this point, our Hematology Dept assists on BMs on the floors. If they > could, they would pass it on to us, but we do not have the staff (or desire) > to do it. My past 2 HT hires came from places where the histotechs assisted > on the BM collection. > Hope that helps! > Wanda > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error > and that any use, dissemination, distribution, forwarding, printing, or > copying of this email or any attached files is strictly prohibited. If you > have received this email in error, please immediately purge it and all > attachments and notify the sender by reply email or contact the sender at > the number listed. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynn L > Sent: Tuesday, July 13, 2010 9:15 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] assisting with patient bone marrows > > > Can anyone provide some input about which department (Hematology, > Histology etc.) assists on the patient floors with bone marrow aspirations > and to what extent? Thanks in advance! > > > > Lynn Lee > > Casa Grande, AZ > > > > _________________________________________________________________ > The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with > Hotmail. > > http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From Carol.Fields <@t> Northside.com Thu Jul 15 08:42:48 2010 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Thu Jul 15 08:42:57 2010 Subject: [Histonet] FW: Fried biopsies Message-ID: <731941C266951A47BEF11E5EFAAED9C904258184@nsmvexch01.northside.local> From: Carol Fields Sent: Thursday, July 15, 2010 9:37 AM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Fried biopsies Hi Histotechs, We have fried GI biopsies this morning and I remember a solution you can soak them in to reprocess them and hopefully make them diagnosable. It has been several years since I used this solution so I do not have the recipe. Does anyone out there ....Derm lab, or anywhere that knows how to back this tissue up to salvage it? Any help with this is greatly appreciated. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From sbaldwin <@t> mhhcc.org Thu Jul 15 08:48:05 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Thu Jul 15 08:48:41 2010 Subject: [Histonet] WASTE DISPOSAL Message-ID: Histonetters I would like to know about this as well! Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From HornHV <@t> archildrens.org Thu Jul 15 08:55:51 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jul 15 08:55:56 2010 Subject: [Histonet] Excluded and Limited Tissues to Histo In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8392E@EMAIL.archildrens.org> I believe this list is generated by your surgical affairs medical staff. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rick.Garnhart@memorialhealthsystem.com Sent: Wednesday, July 14, 2010 3:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Excluded and Limited Tissues to Histo Does anyone know if CAP Today has an article within the last couple of years as to what tissue can be excluded from submission and for gross only exams. The last article I have reference to was from 1996. Thanks Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From kryan <@t> nfderm.com Thu Jul 15 08:58:10 2010 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Thu Jul 15 08:58:15 2010 Subject: [Histonet] FW: Fried biopsies In-Reply-To: <731941C266951A47BEF11E5EFAAED9C904258184@nsmvexch01.northside.local> References: <731941C266951A47BEF11E5EFAAED9C904258184@nsmvexch01.northside.local> Message-ID: This is a procedure that I have used in the past and it has worked very well. Good luck! Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Fields Sent: Thursday, July 15, 2010 9:43 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Fried biopsies From: Carol Fields Sent: Thursday, July 15, 2010 9:37 AM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Fried biopsies Hi Histotechs, We have fried GI biopsies this morning and I remember a solution you can soak them in to reprocess them and hopefully make them diagnosable. It has been several years since I used this solution so I do not have the recipe. Does anyone out there ....Derm lab, or anywhere that knows how to back this tissue up to salvage it? Any help with this is greatly appreciated. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Thu Jul 15 09:02:56 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Jul 15 09:03:02 2010 Subject: [Histonet] Histotech Needed in ATL GA Message-ID: MPath Search Partners, A division of Allied Search Partners is currently looking for histology candidates in the Atlanta, GA area. Position: Moh?s Histotechnician/Histotechnologist Requirements: HT/HTL preferred Moh?s Histology Experience 1-5 years experience in routine histology Environment: Day Shift, Monday-Friday, about 8am-5pm Small Private laboratory To apply: Please submit resume to Alyssa@alliedsearchpartners.com to be considered for a phone interview with one of our recruiters. Your resume is always kept confidential until we have your consent. Visit our website to refer a friend for a generous bonus! -- Alyssa Peterson, Director of Candidate Recruitment Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From joost.bruijntjes <@t> tno.nl Thu Jul 15 09:36:35 2010 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Thu Jul 15 09:36:42 2010 Subject: [Histonet] frozen lung slides Message-ID: <8865601DD17A554CB489C17FFD8A51B203A7D4E2@MAIL04.tsn.tno.nl> Hi all Is anyone of you able to prepare frozen lung slides (7 um) using formalin fixed tissue for IHC? Joost Bruijntjes TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From kryan <@t> nfderm.com Thu Jul 15 09:49:45 2010 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Thu Jul 15 09:49:54 2010 Subject: [Histonet] FW: Fried biopsies In-Reply-To: References: <731941C266951A47BEF11E5EFAAED9C904258184@nsmvexch01.northside.local> Message-ID: Sorry, I forgot about attachments. Here is the procedure. REPROCESSING DRIED OR SUB-OPTIMAL TISSUE SPECIMENS PURPOSE: This procedure provides instructions for rehydrating dried tissue specimens. PRINCIPLE: Tissue may become dry due to a malfunctioning of the processor, or if the tissue has inadvertently been left out of fixative for an extended period of time. The fixative can also sometimes evaporate from the container, or leak out if the lid is not tightly sealed. Some cadaver tissues may be dried out if the body is discovered some days after death has occurred and been exposed to the elements. TEST METHOD INSTRUCTIONS: N/A SPECIMEN TYPE/: Any tissue that is dried or brittle either before or after fixation, or improperly processed paraffin blocks resulting from processor malfunction. EQUIPMENT: Tissue processor (VIP) Graduated cylinders Metal tissue molds Glass containers SAFETY REQUIREMENTS/SPECIAL HANDLING: When reprocessing tissue specimens, gloves, goggles, and a plastic apron or laboratory coat should be worn. Formalin is a suspected carcinogen. Glycerin should be handled under a hood. REAGENTS NEEDED: Formol- Sodium Acetate (Stock) Solution Formol- Glycerol (Working) Solution Paraffin Xylene 100% Dehydrant 95% Dehydrant REAGENT PREPARATION: Formol-Sodium Acetate (Stock) Solution Concentrated formalin (37%) solution____________________50.0 ml Sodium Acetate______________________________________10.0 g Tap water___________________________________________450.0 ml Formol-Glycerol (Working) Solution Formol-Sodium Acetate (Stock) Solution__________________450.0 ml Glycerol (glycerin)____________________________________50.0 ml PROCEDURE: A. If tissue is dry due to a malfunction of the processor, follow steps 6-7 of the reprocessing schedule below. B. If the tissue is left out of fixative for a period of time, or if the fixative evaporates, follow steps 6-7 of the reprocessing schedule below. C. If at microtomy, paraffin blocks do not section due to improper impregnation with paraffin, reprocessing can be accomplished by following all steps of the reprocessing schedule. Reprocessing Schedule: 1. Place paraffin blocks in molten paraffin wax (60 C) for two (2) hours. 2. Remove tissue from paraffin and place in three (3) changes of xylene for one (1) hour each. 3. Place tissue in 100% dehydrant, two (2) changes, for one (1) hour each. 4. Place tissue in 95% dehydrant, two (2) changes, for one (1) hour each. 5. Place tissue under running tap water for 20 minutes. 6. Place tissue in formol-glycerol working solution until softening occurs. (This usually occurs within 5-8 hours. Extended exposure to the formol-glycerol solution will not harm tissue.) 7. Place tissue on the processor and proceed with routine processing starting in the dehydrant. (It is not necessary to expose the tissue to further fixation.) EXPECTED RESULTS: Dried tissue specimens are softened and acceptable histologic preparations can then be generated. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Thursday, July 15, 2010 9:58 AM To: Carol Fields; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: Fried biopsies This is a procedure that I have used in the past and it has worked very well. Good luck! Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Fields Sent: Thursday, July 15, 2010 9:43 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Fried biopsies From: Carol Fields Sent: Thursday, July 15, 2010 9:37 AM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Fried biopsies Hi Histotechs, We have fried GI biopsies this morning and I remember a solution you can soak them in to reprocess them and hopefully make them diagnosable. It has been several years since I used this solution so I do not have the recipe. Does anyone out there ....Derm lab, or anywhere that knows how to back this tissue up to salvage it? Any help with this is greatly appreciated. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Thu Jul 15 09:52:59 2010 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Thu Jul 15 09:53:15 2010 Subject: [Histonet] inking dyes Message-ID: Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. From kmerriam2003 <@t> yahoo.com Thu Jul 15 10:00:09 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Jul 15 10:00:17 2010 Subject: [Histonet] human on human IHC Message-ID: <144973.54434.qm@web50308.mail.re2.yahoo.com> Hi, Does anyone know of a human-on-human IHC kit similar?to the mouse-on-mouse kits that are available? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From flnails <@t> texaschildrens.org Thu Jul 15 10:46:58 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Jul 15 10:47:15 2010 Subject: [Histonet] RE: inking dyes In-Reply-To: References: Message-ID: Statlab or TBS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Thursday, July 15, 2010 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inking dyes Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From bakevictoria <@t> gmail.com Thu Jul 15 10:51:34 2010 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Jul 15 10:51:45 2010 Subject: [Histonet] inking dyes In-Reply-To: References: Message-ID: Try the EMS website I find them useful and their pricing is competative. On Thu, Jul 15, 2010 at 10:52 AM, Diana McCaig wrote: > Can someone provide me with a supplier of tissue marking dyes for use on > the grossing bench. > > Diana McCaig > Histology Lab > Chatham Kent Health Alliance > 80 Grand Avenue West > Chatham. Ontario > N7L 1B7 > 519-352-6401 (6604) > > This email communication and any files transmitted with it may contain > confidential and or proprietary information and is provided for the use > of the intended recipient only. Any review, retransmission or > dissemination of this information by anyone other than the intended > recipient is prohibited. If you receive this email in error, please > contact the sender and delete this communication and any copies > immediately. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sgoebel <@t> xbiotech.com Thu Jul 15 11:27:19 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Thu Jul 15 11:27:24 2010 Subject: [Histonet] human on human IHC Message-ID: <20100715092719.9e2d9aa830e8449a2412eb1e4f2f067e.64c033395c.wbe@email04.secureserver.net> I am doing alot with this right now. The best thing I have do is to conjugate your primary antibody with HRP, then just use the direct method (no secondary), and it turns out beautifully!! You can buy the order number if you need it. Good Luck!! Sarah Goebel, Histotechnician XBiotech US 8201 East Riverside Dr. Bldg 4 Suite 100 A (512)386-5107 -------- Original Message -------- Subject: [Histonet] human on human IHC From: Kim Merriam <[1]kmerriam2 Date: Thu, July 15, 2010 8:00 am To: Histonet <[2]his Hi, Does anyone know of a human-on-human IHC kit similar to the mouse-on-m that are available? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list [3]Histonet@lists.utsou [4]http: References 1. 3D"mailto://kmerriam2003@yahoo.com"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Carol.Fields <@t> Northside.com Thu Jul 15 11:29:47 2010 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Thu Jul 15 11:29:55 2010 Subject: FW: [Histonet] FW: Fried biopsies Message-ID: <731941C266951A47BEF11E5EFAAED9C904258399@nsmvexch01.northside.local> Can we please add this to the HistoNet Archives so we can all find this procedure? See attached........ Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com -----Original Message----- From: Kaye Ryan [mailto:kryan@nfderm.com] Sent: Thursday, July 15, 2010 9:58 AM To: Carol Fields; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: Fried biopsies This is a procedure that I have used in the past and it has worked very well. Good luck! Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Fields Sent: Thursday, July 15, 2010 9:43 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Fried biopsies From: Carol Fields Sent: Thursday, July 15, 2010 9:37 AM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Fried biopsies Hi Histotechs, We have fried GI biopsies this morning and I remember a solution you can soak them in to reprocess them and hopefully make them diagnosable. It has been several years since I used this solution so I do not have the recipe. Does anyone out there ....Derm lab, or anywhere that knows how to back this tissue up to salvage it? Any help with this is greatly appreciated. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From JKELL2 <@t> capefearvalley.com Thu Jul 15 11:34:31 2010 From: JKELL2 <@t> capefearvalley.com (Jason Keller) Date: Thu Jul 15 11:34:39 2010 Subject: [Histonet] Histotech opportunities in NC Message-ID: <7D41E97C787DF44996E0055C8170366301263579@ntexchange3.capefear.local> Histotech positions open at Cape Fear Valley Health System in Fayetteville, NC. Please contact me for more information or apply on the hospitals webpage. Thank you, Jason Keller Technical Specialist Cape Fear Valley Health System Fayetteville, NC CONFIDENTIALITY NOTICE: This electronic mail transmission may contain information that is privileged and/or confidential. Additionally, this communication may contain individual protected health information ("PHI") that is subject to protection under state and federal laws, or other privileged, confidential or proprietary information of Cape Fear Valley Health System that may not be further disclosed. Please be advised that any disclosure, copying, distribution or other use of the contents of this message by anyone other than the intended recipient is prohibited. If you have received this communication in error, please notify the sender immediately by replying to the message and deleting it from your computer. From annataylor <@t> hotmail.com Thu Jul 15 11:36:43 2010 From: annataylor <@t> hotmail.com (Anna Taylor) Date: Thu Jul 15 11:36:47 2010 Subject: [Histonet] Hemoglobin Stain Message-ID: Hi all, In my quest for advice about what will be my very first attempt at histology, I have been recommended to post my query here. I'm attempting to demonstrate hemoglobin in FFPE lymph node sections. I've been able to find a couple of techniques (namely the Dunn & Thompson technique and Okajima's technique), although I'm not sure how suitable either would be on lymph node sections? As I said, this will be my debut experience in histology/histochemistry so any advice/recommendations at all will be appreciated :)Thanks,Anna. _________________________________________________________________ View photos of singles in your area! Looking for a hot date? http://clk.atdmt.com/NMN/go/150855801/direct/01/ From LSebree <@t> uwhealth.org Thu Jul 15 11:41:54 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Jul 15 11:41:59 2010 Subject: [Histonet] Hemoglobin Stain In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E2738399F0A@UWHC-MAIL01.uwhis.hosp.wisc.edu> Anna, Are you limited to histochemical stains? Because if you're not, hemoglobin may be detected by immunohistochemistry quite nicely. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna Taylor Sent: Thursday, July 15, 2010 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hemoglobin Stain Hi all, In my quest for advice about what will be my very first attempt at histology, I have been recommended to post my query here. I'm attempting to demonstrate hemoglobin in FFPE lymph node sections. I've been able to find a couple of techniques (namely the Dunn & Thompson technique and Okajima's technique), although I'm not sure how suitable either would be on lymph node sections? As I said, this will be my debut experience in histology/histochemistry so any advice/recommendations at all will be appreciated :)Thanks,Anna. _________________________________________________________________ View photos of singles in your area! Looking for a hot date? http://clk.atdmt.com/NMN/go/150855801/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Thu Jul 15 11:44:34 2010 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Jul 15 11:45:17 2010 Subject: [Histonet] RE: inking dyes In-Reply-To: References: , Message-ID: <85F2E7DD5D91744D831A66E44D718B9A138BDC87@fhovxchmb7001.ADVENTISTCORP.NET> Davidson Marking Systems ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton [flnails@texaschildrens.org] Sent: Thursday, July 15, 2010 11:46 AM To: 'Diana McCaig'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: inking dyes Statlab or TBS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Thursday, July 15, 2010 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inking dyes Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Rhonda.Hartz <@t> saskatoonhealthregion.ca Thu Jul 15 11:42:26 2010 From: Rhonda.Hartz <@t> saskatoonhealthregion.ca (Hartz, Rhonda SktnHR) Date: Thu Jul 15 11:58:41 2010 Subject: [Histonet] Wax removal Message-ID: Hi everyone; This may seem like a insignificant question, but we consistently have issues with injuries to our maintenance staff from cleaning the wax off of our floors. Housekeeping has laid sticky layered mats all over our floors (like large mouse traps), which they peel off layer by layer as they become wax covered. Apparently these are very expensive. Does anyone have any suggestions? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca From trathborne <@t> somerset-healthcare.com Thu Jul 15 12:04:59 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Jul 15 12:08:04 2010 Subject: [Histonet] Wax removal In-Reply-To: Message-ID: We have a paraffin scraper that our Housekeeping staff uses. It was purchased from American MasterTech Scientific (item CPW04200E, and replacement blades item CPW04201P). We also have perforated anti-fatigue mats in aisles around the cutting stations which trap the wax and can be vacuumed/swept away when the mat is flipped. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Hartz, Rhonda SktnHR Sent: Thursday, July 15, 2010 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wax removal Hi everyone; This may seem like a insignificant question, but we consistently have issues with injuries to our maintenance staff from cleaning the wax off of our floors. Housekeeping has laid sticky layered mats all over our floors (like large mouse traps), which they peel off layer by layer as they become wax covered. Apparently these are very expensive. Does anyone have any suggestions? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From thisisann <@t> aol.com Thu Jul 15 12:16:53 2010 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Thu Jul 15 12:17:17 2010 Subject: [Histonet] Water Testing Message-ID: <8CCF258A75C0EFC-178C-158C@webmail-d009.sysops.aol.com> Does anyone know if the following are the current CAP requirements for water testing? The following specification for CLRW (Clinical Laboratory Reagent Water) should be met at time of in-house production: CLRW Maximum microbial content (CFU/mL) 10 Minimum resistivity (megohm-cm) 10 (in-line) Particulate matter 0.22 um filter Thank you, Ann From Cathy.Crumpton <@t> tuality.org Thu Jul 15 12:34:43 2010 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Thu Jul 15 12:34:49 2010 Subject: [Histonet] Can anyone use some reagent? Message-ID: Hello histonet. I have some Dako reagets that we do not nee anymore. We have switched from the LSAB2 system to the Flex polymer and have 2 unopened containers of dilutent (S0809) and one unopened contai pay for sh than thro I also have a barely used container of Halt waterbath ad someone can have if they want it. Our lab did not like th chemical odor given off by the additive. Please email me off Cathy Crumpton HT(ASCP), Histology LeadTuality Community Hospital Hillsboro, OR 97123 (503)681-1292 From hymclab.hymclab <@t> ministryhealth.org Thu Jul 15 13:03:34 2010 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Thu Jul 15 13:04:03 2010 Subject: [Histonet] RE: inking dyes In-Reply-To: <85F2E7DD5D91744D831A66E44D718B9A138BDC87@fhovxchmb7001.ADVENTISTCORP.NET> References: , <85F2E7DD5D91744D831A66E44D718B9A138BDC87@fhovxchmb7001.ADVENTISTCORP.NET> Message-ID: We use Thermo Fisher ones from Fisher HealthCare. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Thursday, July 15, 2010 11:45 AM To: Nails, Felton; 'Diana McCaig'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: inking dyes Davidson Marking Systems ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton [flnails@texaschildrens.org] Sent: Thursday, July 15, 2010 11:46 AM To: 'Diana McCaig'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: inking dyes Statlab or TBS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Thursday, July 15, 2010 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inking dyes Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From alyssa <@t> alliedsearchpartners.com Thu Jul 15 13:10:05 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Jul 15 13:10:11 2010 Subject: [Histonet] Histology Job in WI Message-ID: MPath Search Partners is looking for a Histotechncian/Histotechnologist candidate for permanent direct hire in Wisconsin. *Position: *histotechnician/histotechnologist *Location: *state of the art laboratory in Manitowoc, Wisconsin *Requirements:* HT/HTL ASCP preferred Degree preferred Mohs experience *Benefits:* * Generous PTO accrual schedule & paid holidays * 401k match & profit sharing * Competitive wages/benefits * No holidays * Paid training *Application:* To be considered please submit your resume in order to schedule a phone screen with one of our recruiters. Please be aware that all resumes submitted to Allied Search Partners are confidential. *Serious inquiries only please.* 1. No resume will be submitted until we speak to you for a phone screen. 2. No resume will be submitted to client if you are currently or previously worked for client. 3. Name, and contact information will not be given out without your consent. Please submit resume to Alyssa@alliedsearchpartners.com -- Alyssa Peterson, Director of Candidate Recruitment Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From shirleysgulfcoastdermatology <@t> yahoo.com Thu Jul 15 13:21:49 2010 From: shirleysgulfcoastdermatology <@t> yahoo.com (Shirley Schroyer) Date: Thu Jul 15 13:22:42 2010 Subject: [Histonet] histotechnician needed Message-ID: <488180.13730.qm@web114606.mail.gq1.yahoo.com> ________________________________ Panama City, Florida is seeking a Histotechnician for growing dermatology practice.Responsibilities include grossing, processing, embedding, cutting, staining, and cover-slipping of skin specimens. HT (ASCP) certification preferred, no Florida license required. New grads welcome. Lab is located on the whitem sandy beaches of Panama City. Position includes excellent benefits. Drug and criminal background screening are required. For more information contact Shirley Schroyer at:? shirleysgulfcoastdermatology@yahoo.com or (850) 233-3376 ext. 1028. From MAUGER <@t> email.chop.edu Thu Jul 15 14:31:38 2010 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Thu Jul 15 14:33:01 2010 Subject: [Histonet] RE: thanks Message-ID: <443F5B475A9BF647AB962E834884EBAD278D127817@EX7CCRPW03V1.chop.edu> Thanks to all who answered about mouse macrophages!. I will try F4/80. Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth [RCazares@schosp.org] Sent: Wednesday, July 14, 2010 4:00 PM To: Joanne Clark; histonet@lists.utsouthwestern.edu Subject: RE: [SPAM-HC] - [Histonet] Microtome Aligner - Email found in subject Hello Histonetters, I have just received the microtome aligner tool from TechOne that Newcomer has. I have used it on my three microtomes and let me tell you guys, I LOVE IT!! It is very easy to use, just follow the instructions that come with it. I strongly recommend it (but that's just my opinion). Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Wednesday, July 14, 2010 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] Microtome Aligner - Email found in subject Hi All, I just want to clarify that the microtome aligner that we are having issues with is the one made by Advanced Innovations and sold by Newcomer Supply not the one made by Tech One (Shane Perkins) which Newcomer also sells. I have had no experience with the device that Shane is referring to. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.D.Kobus <@t> Medstar.net Thu Jul 15 15:04:43 2010 From: Robert.D.Kobus <@t> Medstar.net (Robert.D.Kobus@Medstar.net) Date: Thu Jul 15 15:04:52 2010 Subject: [Histonet] Robert D Kobus is out of the Laboratory. Message-ID: I will be out of the office starting Thu 07/15/2010 and will not return until Mon 07/19/2010. I will be out of the Laboratory from Friday 7/15/2010 until 7/19/2010. If you have a matter that can not wait, please page me at 202-801-4851 or call my cell phone at 301-832-7626. Otherwise I will return your e-mail on Monday! CONFIDENTIAL: The information contained in this communication, including its attachments may contain confidential information and is intended only for the individual (s) or entity (ies) to whom it is addressed . The information contained in this communication may also be protected by legal privilege , federal law or other applicable law. If you are not the intended recipient of this communication , you are hereby notified that any distribution, dissemination or duplication of this communication is strictly prohibited. If you have received this communication in error please immediately delete and destroy all copies of this message and please immediately notify us of the error by separate communication . Thank you. From Kim.Donadio <@t> bhcpns.org Thu Jul 15 15:20:54 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Thu Jul 15 15:21:06 2010 Subject: [Histonet] assisting with patient bone marrows In-Reply-To: Message-ID: Amen! >>>>> Can't get no respect, I tell ya! Just no respect! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Anne van Binsbergen Sent by: histonet-bounces@lists.utsouthwestern.edu 07/15/2010 07:25 AM To Susan.Walzer@hcahealthcare.com cc histonet@lists.utsouthwestern.edu, Wanda.Smith@hcahealthcare.com Subject Re: [Histonet] assisting with patient bone marrows Oh my - how this makes me smile poor Hema are SUCH busy ones and we in Histo sit around just longing to be able to participate in the 'real' action clinical stuff we are just the dumping ground for all the crap (no disrespect intended to patient samples) that 'others' dont feel like doing. We even get Semen analysis in the Cytology section because at that time the head of Hema refused all body fluids. Now they are doing fluids but Histo is still stuck with the Semens As for the rest of their work, we do the trehines and the specials on the aspirate smears but they do all their own collections. Hema was my first field of speciality so i know what they do - but they have no clue what I do. we print labels for them, help them when their regents run out or expire, supply solvents so they can get their oily mess off the scope objectives - they are not allowed to use the multiheader because of objective 'contamination' when it came to getting their Flow Cytometry built out in Cerner, again we stepped in and did the whole lot. our lab amanger used to be the lead tech in Hema so they are very dear to him - budget and staffing always swings their way. Histo gets the crumbs after all the other sections take their slice - LOL But...I have the best team in the building thats my end-of-week-rant and i feel better for sharing my weekend starts here have a happy friday and enjoy your weekend AnnieinArabia On 15 July 2010 11:18, wrote: > Everyplace I have worked it was done by hematology. When hemo tried to dump > this job on us the pathologist stepped in to remind them that this is their > area of expertise. We do not expect hemo to do frozen sections and we do not > do bone marrows. After they have made their smears and sent the flow they > drop off the core and asp. and one smear for iron to us. We handle decaling > the bone. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Wanda.Smith@HCAhealthcare.com > Sent: Wednesday, July 14, 2010 8:29 AM > To: lynnlee2010@live.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] assisting with patient bone marrows > > Good Morning, > At this point, our Hematology Dept assists on BMs on the floors. If they > could, they would pass it on to us, but we do not have the staff (or desire) > to do it. My past 2 HT hires came from places where the histotechs assisted > on the BM collection. > Hope that helps! > Wanda > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error > and that any use, dissemination, distribution, forwarding, printing, or > copying of this email or any attached files is strictly prohibited. If you > have received this email in error, please immediately purge it and all > attachments and notify the sender by reply email or contact the sender at > the number listed. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynn L > Sent: Tuesday, July 13, 2010 9:15 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] assisting with patient bone marrows > > > Can anyone provide some input about which department (Hematology, > Histology etc.) assists on the patient floors with bone marrow aspirations > and to what extent? Thanks in advance! > > > > Lynn Lee > > Casa Grande, AZ > > > > _________________________________________________________________ > The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with > Hotmail. > > http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From maryam_kherad <@t> yahoo.com Thu Jul 15 15:31:25 2010 From: maryam_kherad <@t> yahoo.com (Maryam) Date: Thu Jul 15 15:31:30 2010 Subject: [Histonet] NG2 antibody/ Antigen retrieval/Protocol Message-ID: <148677.68788.qm@web53908.mail.re2.yahoo.com> Hello, I am trying to stain human glioma sections(as a positive control) using Anti-NG2 antibody. The tissue that I have is Paraffin embedded and I appreciate if somebody could send me the protocol for antigen retrieval ,antibody titer and IHC protocol. Thanks, Maryam From Tom_Wells <@t> bcit.ca Thu Jul 15 15:47:52 2010 From: Tom_Wells <@t> bcit.ca (Tom Wells) Date: Thu Jul 15 15:48:06 2010 Subject: [Histonet] Wax removal In-Reply-To: References: Message-ID: Hi Rhonda, Where do you get the "anti-fatigue mats" Thanks. Tom Tom Wells BSc, ART Faculty School of Medical Laboratory Sciences British Columbia Institute of Technology Burnaby, BC Canada -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- To: "Hartz, Rhonda SktnHR" , From: "Rathborne, Toni" Sent by: histonet-bounces@lists.utsouthwestern.edu Date: 07/15/2010 10:04AM Subject: RE: [Histonet] Wax removal We have a paraffin scraper that our Housekeeping staff uses. It was purchased from ? American MasterTech Scientific (item CPW04200E, and replacement blades item CPW04201P). We also have perforated anti-fatigue mats in aisles around the cutting stations which trap the wax and can be vacuumed/swept away when the mat is flipped. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Hartz, Rhonda SktnHR Sent: Thursday, July 15, 2010 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wax removal Hi everyone; This may seem like a insignificant question, but we consistently have issues with injuries to our maintenance staff from cleaning the wax off of our floors. ?Housekeeping has laid sticky layered mats all over our floors (like large mouse traps), which they peel off layer by layer as they become wax covered. ?Apparently these are very expensive. ?Does anyone have any suggestions? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. ?The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. ?Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. ?If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Jul 15 20:06:11 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Jul 15 20:06:15 2010 Subject: [Histonet] Re: inking dyes Message-ID: Diana McCaig in Chatham, Ontario asks >>Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench.<< You're referring to inks used to mark the margins of resected tissue so that the pathologist can find the surgical margins under the microscope. They're particulate inks suspended in a viscous medium, rather than soluble dyes. There are several brands of these inks. In my personal experience the Davison marking inks (see bradleyproducts.com) - order direct from them or through some of the standard vendors - are better than the competition. (I have no commercial connection with Bradley Products.) Some pathologists like tattoo inks - available in great numbers of colors and quite cheap - but the catalogs you have to buy them from are a real gross-out. Most important thing about using any marking ink is to cap the bottle promptly when you're through using it. Do that and they'll last for years. Bob Richmond Samurai Pathologist Knoxville TN From Wanda.Smith <@t> HCAhealthcare.com Fri Jul 16 09:00:01 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Jul 16 09:00:01 2010 Subject: [Histonet] Wax removal In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA138F3063F9@NADCWPMSGCMS03.hca.corpad.net> We get our anti-fatigue mats from Lab Safety Supply. They have an extra thick one that I highly recommend. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom Wells Sent: Thursday, July 15, 2010 4:48 PM To: Rathborne, Toni Cc: histonet@lists.utsouthwestern.edu; Hartz, Rhonda SktnHR Subject: RE: [Histonet] Wax removal Hi Rhonda, Where do you get the "anti-fatigue mats" Thanks. Tom Tom Wells BSc, ART Faculty School of Medical Laboratory Sciences British Columbia Institute of Technology Burnaby, BC Canada -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- To: "Hartz, Rhonda SktnHR" , From: "Rathborne, Toni" Sent by: histonet-bounces@lists.utsouthwestern.edu Date: 07/15/2010 10:04AM Subject: RE: [Histonet] Wax removal We have a paraffin scraper that our Housekeeping staff uses. It was purchased from ? American MasterTech Scientific (item CPW04200E, and replacement blades item CPW04201P). We also have perforated anti-fatigue mats in aisles around the cutting stations which trap the wax and can be vacuumed/swept away when the mat is flipped. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Hartz, Rhonda SktnHR Sent: Thursday, July 15, 2010 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wax removal Hi everyone; This may seem like a insignificant question, but we consistently have issues with injuries to our maintenance staff from cleaning the wax off of our floors. ?Housekeeping has laid sticky layered mats all over our floors (like large mouse traps), which they peel off layer by layer as they become wax covered. ?Apparently these are very expensive. ?Does anyone have any suggestions? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. ?The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. ?Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. ?If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annataylor <@t> hotmail.com Fri Jul 16 09:52:39 2010 From: annataylor <@t> hotmail.com (Anna Taylor) Date: Fri Jul 16 09:52:47 2010 Subject: [Histonet] Hemoglobin Stain In-Reply-To: <8C023B4AB999614BA4791BAEB26E2738399F0A@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: , <8C023B4AB999614BA4791BAEB26E2738399F0A@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: Hi Linda, Thanks for the reply! No, I'm not limited to staining only. In fact, I will be performing some seperate IHC to demonstrate a couple of other proteins. It is simply my preference to use a histochemical technique for the following reasons. Firstly, from what I understand, staining will not be as laborious as IHC nor as difficult/technical to obtain positive results. Secondly, as this is part of my Honours year project, I would like to try my luck with several different techniques, and hemoglobin is the only molecule from the products I am looking at for which a simple histochemical approach is possible. Again, any suggestions would be highly valuable. Cheers, Anna > Subject: RE: [Histonet] Hemoglobin Stain > Date: Thu, 15 Jul 2010 11:41:54 -0500 > From: LSebree@uwhealth.org > To: annataylor@hotmail.com; histonet@lists.utsouthwestern.edu > > Anna, > > Are you limited to histochemical stains? Because if you're not, > hemoglobin may be detected by immunohistochemistry quite nicely. > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna > Taylor > Sent: Thursday, July 15, 2010 11:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hemoglobin Stain > > > Hi all, > > In my quest for advice about what will be my very first attempt at > histology, I have been recommended to post my query here. > > I'm attempting to demonstrate hemoglobin in FFPE lymph node sections. > I've been able to find a couple of techniques (namely the Dunn & > Thompson technique and Okajima's technique), although I'm not sure how > suitable either would be on lymph node sections? As I said, this will be > my debut experience in histology/histochemistry so any > advice/recommendations at all will be appreciated :)Thanks,Anna. > > _________________________________________________________________ > View photos of singles in your area! Looking for a hot date? > http://clk.atdmt.com/NMN/go/150855801/direct/01/________________________ > _______________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ If It Exists, You'll Find it on SEEK. Australia's #1 job site http://clk.atdmt.com/NMN/go/157639755/direct/01/ From kgrobert <@t> rci.rutgers.edu Fri Jul 16 10:23:37 2010 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Fri Jul 16 10:23:40 2010 Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin block Message-ID: Is there a way to do this without one or more pieces falling over? I saw in the archive the method for frozen sections-embed them on their sides in OCT, then cut on the end, but I don't think I'd be able to do that in paraffin. Would one of the tissue microarray methods work? (I've never done that before, so I have no idea.) Thanks in advance for all your help, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 From sgoebel <@t> xbiotech.com Fri Jul 16 10:56:33 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Jul 16 10:56:47 2010 Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin block Message-ID: <20100716085633.9e2d9aa830e8449a2412eb1e4f2f067e.5adf627c1b.wbe@email04.secureserver.net> How many are we talking about? I embed 6 sections of mouse end and it works? Just fill the mold, put it on the cold spo second, then on some room temperature area, the paraffin will harde slowly enough that you should be able to embed them? Sarah Goebel, B.A., Histotechnician < XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, T (512)386-5107 < -------- Original Message -------- Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin block From: [1]kgrobert@rci.rutgers.ed Date: Fri, July 16, 2010 8:23 am To: "histonet" <[2]h Is there a way to do this without one or more pieces falling over? I saw in the archive the method for frozen sections-embed them on their sides in< to do that in paraffin. Would one of the tissue microarray methods work? (I've never done that before, so I have no idea.) Thanks in advance for all your help, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 _______________________________________________ Histonet mailing list [3]Histonet@lists.utsou [4]http: References 1. 3D"mailto://kgrobert@rci.rutgers.edu"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From mpence <@t> grhs.net Fri Jul 16 11:00:01 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Jul 16 11:00:06 2010 Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin block In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3E77@is-e2k3.grhs.net> I have techs that embed 10-15 pieces on end in one block. Just cool the block slowly and move your pieces around quickly. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu Sent: Friday, July 16, 2010 10:24 AM To: histonet Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin block Is there a way to do this without one or more pieces falling over? I saw in the archive the method for frozen sections-embed them on their sides in OCT, then cut on the end, but I don't think I'd be able to do that in paraffin. Would one of the tissue microarray methods work? (I've never done that before, so I have no idea.) Thanks in advance for all your help, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From napoli <@t> siscom.net Fri Jul 16 11:06:08 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Fri Jul 16 11:06:11 2010 Subject: [Histonet] Paraffin Block Storage Message-ID: <4c408370.bc.46d3.1478974399@siscom.net> Hello histonetters, Does anyone know of a good source of used or economical plastic storage drawer cabinets for paraffin blocks? Auctions, used equipment, or otherwise retailers? Interested in purchasing some. We have thousands of blocks to store. Thanks! AB From 41dmb41 <@t> gmail.com Fri Jul 16 11:07:20 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Fri Jul 16 11:07:45 2010 Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin block In-Reply-To: References: Message-ID: We regularly embed 6-12 pieces on end in one block without any special method. You just have to be quick... and don't leave the block on the cold plate very long... just touch the cold plate briefly while embedding the individual piece, then lift the block off the plate until you grab the next piece... repeat quickly and you'll run out of room in the mold before you'll have to worry about it hardening too much. Drew On Fri, Jul 16, 2010 at 11:23, wrote: > Is there a way to do this without one or more pieces falling over? I saw > in the archive the method for frozen sections-embed them on their sides in > OCT, then cut on the end, but I don't think I'd be able to do that in > paraffin. Would one of the tissue microarray methods work? (I've never > done that before, so I have no idea.) > > Thanks in advance for all your help, > Kathleen > > > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (732) 445-6914 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Lynne.Bell <@t> cvmc.org Fri Jul 16 11:31:35 2010 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Fri Jul 16 11:31:40 2010 Subject: [Histonet] Paraffin Block Storage In-Reply-To: <4c408370.bc.46d3.1478974399@siscom.net> Message-ID: In Vermont we are very frugal. What I have used for more years than I care to remember are pizza boxes from Pizza Hut. Our local Pizza Hut gives them to us for free. They are very sturdy and perfectly fit 11 rows across and approximately 36 blocks per row. I have 20 years of blocks stored this way. Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From brian <@t> prometheushealthcare.com Fri Jul 16 11:44:26 2010 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Fri Jul 16 11:44:31 2010 Subject: [Histonet] Lab in Aiken, South Carolina seeking histotech Message-ID: <002401cb2506$27542ad0$75fc8070$@com> Outpatient laboratory located in Aiken South Carolina is currently seeking a histotech. The company includes 20 specialty pathologists, more than 100 dedicated professionals, operates 9 patient service centers, and provides medical directorship for 18 hospitals throughout Georgia and northern Florida. Please contact me today for consideration Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From kgrobert <@t> rci.rutgers.edu Fri Jul 16 12:45:35 2010 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Fri Jul 16 12:45:39 2010 Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin block In-Reply-To: <3E41B7C70659AD4D9429EAF86B23F0A6380A736724@EMAIL01.CCHCS.LDAP> References: <3E41B7C70659AD4D9429EAF86B23F0A6380A736724@EMAIL01.CCHCS.LDAP> Message-ID: <606c93c5ae6cafec39b58c82b5b634ea.squirrel@webmail.rci.rutgers.edu> To all, OK, looks like most of you are saying the same thing-work fast! :o) I've printed out all of your replies and discussed them with the graduate student who needs this done for her thesis, and she agrees with me-it's just going to take practice. I'll try the "cool slowly/work fast" suggestions first and see how that goes, then see about the trick with the cucumber (which sounds really cool!), slowly edging the mold onto the cold plate as I go, and using sponges to help flip the samples on end (and not necessarily in that order). I don't want to try too many things at once, lest I drive myself nuts. The first batch of samples should be coming sometime next week. Wish me luck, and thanks for all your help! Kathy From cpyse <@t> x-celllab.com Fri Jul 16 13:04:36 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Jul 16 13:05:04 2010 Subject: [Histonet] inking dyes In-Reply-To: References: Message-ID: <000801cb2511$5a03a090$0e0ae1b0$@com> I use tattoo dyes. They work great and are much cheaper than tissue marking dyes. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Thursday, July 15, 2010 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inking dyes Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Fri Jul 16 14:06:11 2010 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Jul 16 14:06:57 2010 Subject: [Histonet] Re: NG2 antibody/ Antigen retrieval/Protocol Message-ID: <11D9615B89C10747B1C985966A63D7CA2D7BAE9949@KCL-MAIL04.kclad.ds.kcl.ac.uk> "I am trying to stain human glioma sections(as a positive control) using Anti-NG2 antibody. The tissue that I have is Paraffin embedded and I appreciate if somebody could send me the protocol for antigen retrieval ,antibody titer and IHC protocol. " Let us know what Ab you are currently using ( Source and catalogue number) and also what detection/AR protocol you are using. Also, what problem do you have using your Ab? Best wishes, Carl From victor <@t> pathology.washington.edu Fri Jul 16 14:33:32 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Jul 16 14:34:16 2010 Subject: [Histonet] Cytology 100 slide limit Message-ID: <4C40B40C.7080501@pathology.washington.edu> Our Cytology Supervisor was telling me about the 100 slide maximum that they can screen in a day. Our LIS is not capturing the NON-GYN slides being screened, so unless you are very diligent in recording the slides screened, you could go over the 100 limit. Our supervisor also believes the computer system should notify the user when the limit has been reached and prevent them from continuing. Is this a CAP requirement? How are you dealing with this problem or is it a problem for you? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From rjbuesa <@t> yahoo.com Fri Jul 16 16:20:30 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 16 16:20:34 2010 Subject: [Histonet] Cytology 100 slide limit Message-ID: <374659.64380.qm@web65709.mail.ac4.yahoo.com> Victor: As you wrote, either you?have to be?diligent in recording your work, or you will have to?modify the software of the LIS system to account correctly. On the other hand, if you are dealing with liquid base samples usually using a Thin-Prep Imaging System (TIS) and the sample covers one-half or less of the slide surface, CLIA88 has expanded the limit from 100 to 200 slides/day. Ren? J. --- On Fri, 7/16/10, Victor Tobias wrote: From: Victor Tobias Subject: [Histonet] Cytology 100 slide limit To: "Histonet" Date: Friday, July 16, 2010, 3:33 PM Our Cytology Supervisor was telling me about the 100 slide maximum that they can screen in a day. Our LIS is not capturing the NON-GYN slides being screened, so unless you are very diligent in recording the slides screened, you could go over the 100 limit. Our supervisor also believes the computer system should notify the user when the limit has been reached and prevent them from continuing. Is this a CAP requirement? How are you dealing with this problem or is it a problem for you? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jan.Minshew <@t> leica-microsystems.com Fri Jul 16 16:43:11 2010 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Fri Jul 16 16:45:51 2010 Subject: [Histonet] New Job Opportunities Message-ID: Hello everyone and happy Friday to all, Leica Microsystems is adding new positions and would like to hear from those of you who might be interested. The positions are for Field Support Specialists (FSS) and will be based in the following areas: Southeast Florida NYC/Bronx/NJ MD/VA/DC TN/MS/AR NM/TX/western OK CO/WY/MT Southern IL/Iowa Some of the responsibilities include in-field support for IHC, conducting demonstrations and post-sales customer training, performing validations and optimization, and providing technical support for remote problem solving. If you are interested in trying something different and feel that you are qualified, please contact Linda Jordan, HR Recruiter at hrcoord@leica-microsystems.com. Kind regards, Jan Minshew Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 Office: 847.405.7051 Cell: 847.970.8468 Fax: 847.405.6560 www.leica-microsystems.com Click Here for this month's special offers! http://www.leica-microsystems.com/bsdspecial ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From sfeher <@t> CMC-NH.ORG Fri Jul 16 16:57:59 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Jul 16 16:58:03 2010 Subject: [Histonet] Cytology 100 slide limit In-Reply-To: <374659.64380.qm@web65709.mail.ac4.yahoo.com> References: <374659.64380.qm@web65709.mail.ac4.yahoo.com> Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B75647@exchange.cmc-nh.org> Victor, Rene is correct in stating that CLIA allows Gyn Liquid Based Paps to be counted as 1/2 slide. It gets tricky when you start mixing 1/2 slide counts and full slide counts in making sure the techs do not exceed 100 slides (or in the case of LBP's 200 slides). If you get a lot of FNA's the counts can go up very quickly. Most LIS systems can prevent Techs from exceeding their limit. I would check again to see why your LIS is not capturing the NON-Gyn slides. Another CAP and CLIA requirement is that each 6 months, the cytotechs are supposed to have a Competency Assessment where the Medical Director signs off on the maximum number of slides each tech is qualified to screen. This is the number that most labs place in the LIS as the max that particular tech can review. What LIS system do you have? Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, July 16, 2010 5:21 PM To: Histonet; Victor Tobias Subject: Re: [Histonet] Cytology 100 slide limit Victor: As you wrote, either you?have to be?diligent in recording your work, or you will have to?modify the software of the LIS system to account correctly. On the other hand, if you are dealing with liquid base samples usually using a Thin-Prep Imaging System (TIS) and the sample covers one-half or less of the slide surface, CLIA88 has expanded the limit from 100 to 200 slides/day. Ren? J. --- On Fri, 7/16/10, Victor Tobias wrote: From: Victor Tobias Subject: [Histonet] Cytology 100 slide limit To: "Histonet" Date: Friday, July 16, 2010, 3:33 PM Our Cytology Supervisor was telling me about the 100 slide maximum that they can screen in a day. Our LIS is not capturing the NON-GYN slides being screened, so unless you are very diligent in recording the slides screened, you could go over the 100 limit. Our supervisor also believes the computer system should notify the user when the limit has been reached and prevent them from continuing. Is this a CAP requirement? How are you dealing with this problem or is it a problem for you? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sweething63 <@t> msn.com Fri Jul 16 17:48:03 2010 From: sweething63 <@t> msn.com (R J VAZQUEZ) Date: Fri Jul 16 17:48:07 2010 Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin block In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3E77@is-e2k3.grhs.net> References: , <661949901A768E4F9CC16D8AF8F2838C017A3E77@is-e2k3.grhs.net> Message-ID: Hello, The way I used to embed GI specimens is to place them on end on a stack of specimen bags that have solidified, since they are already in place, then you can pick them up quickly. I did this with sectioned arterial artieries or vas deferens this worked like a dream. Robyn Vazquez > Date: Fri, 16 Jul 2010 11:00:01 -0500 > From: mpence@grhs.net > To: kgrobert@rci.rutgers.edu; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block > CC: > > I have techs that embed 10-15 pieces on end in one block. Just cool the > block slowly and move your pieces around quickly. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > kgrobert@rci.rutgers.edu > Sent: Friday, July 16, 2010 10:24 AM > To: histonet > Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin > block > > > Is there a way to do this without one or more pieces falling over? I > saw in the archive the method for frozen sections-embed them on their > sides in OCT, then cut on the end, but I don't think I'd be able to do > that in paraffin. Would one of the tissue microarray methods work? > (I've never done that before, so I have no idea.) > > Thanks in advance for all your help, > Kathleen > > > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (732) 445-6914 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Fri Jul 16 20:04:18 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Jul 16 20:04:21 2010 Subject: [Histonet] Wax removal Message-ID: Hi, You can get abrasive pads that have adhesive on one side. They are usually used on stairs. They are like 8 inch wide 3 foot long strips of sandpaper that stick (really well) to the floor. I have them placed about 1 per foot along main traffic areas. I don't recall off the top of my head where we got them from, but if you'd like, I'll look it up on Monday. Amos Message: 6 Date: Thu, 15 Jul 2010 10:42:26 -0600 From: "Hartz, Rhonda SktnHR" Subject: [Histonet] Wax removal To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi everyone; This may seem like a insignificant question, but we consistently have issues with injuries to our maintenance staff from cleaning the wax off of our floors. Housekeeping has laid sticky layered mats all over our floors (like large mouse traps), which they peel off layer by layer as they become wax covered. Apparently these are very expensive. Does anyone have any suggestions? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca From conniegrubaugh <@t> hotmail.com Fri Jul 16 21:50:41 2010 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Fri Jul 16 21:50:46 2010 Subject: [Histonet] Wax removal In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA138F3063F9@NADCWPMSGCMS03.hca.corpad.net> References: , , <9E2D36CE2D7CBA4A94D9B22E8328A3BA138F3063F9@NADCWPMSGCMS03.hca.corpad.net> Message-ID: We get our anti-fatigue mats from Sam's make sure you get the red ones and not the black ones. They are in the office materials area. Connie G. > From: Wanda.Smith@HCAhealthcare.com > To: Tom_Wells@bcit.ca; trathborne@somerset-healthcare.com > Date: Fri, 16 Jul 2010 09:00:01 -0500 > Subject: RE: [Histonet] Wax removal > CC: histonet@lists.utsouthwestern.edu; Rhonda.Hartz@saskatoonhealthregion.ca > > We get our anti-fatigue mats from Lab Safety Supply. They have an extra thick one that I highly recommend. > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom Wells > Sent: Thursday, July 15, 2010 4:48 PM > To: Rathborne, Toni > Cc: histonet@lists.utsouthwestern.edu; Hartz, Rhonda SktnHR > Subject: RE: [Histonet] Wax removal > > > Hi Rhonda, > > Where do you get the "anti-fatigue mats" Thanks. Tom > > Tom Wells BSc, ART > Faculty > School of Medical Laboratory Sciences > British Columbia Institute of Technology Burnaby, BC Canada > > -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- > > To: "Hartz, Rhonda SktnHR" , > > From: "Rathborne, Toni" > Sent by: histonet-bounces@lists.utsouthwestern.edu > Date: 07/15/2010 10:04AM > Subject: RE: [Histonet] Wax removal > > We have a paraffin scraper that our Housekeeping staff uses. It was purchased from American MasterTech Scientific (item CPW04200E, and replacement blades item CPW04201P). We also have perforated anti-fatigue mats in aisles around the cutting stations which trap the wax and can be vacuumed/swept away when the mat is flipped. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Hartz, Rhonda SktnHR > Sent: Thursday, July 15, 2010 12:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wax removal > > > Hi everyone; > > This may seem like a insignificant question, but we consistently have issues with injuries to our maintenance staff from cleaning the wax off of our floors. Housekeeping has laid sticky layered mats all over our floors (like large mouse traps), which they peel off layer by layer as they become wax covered. Apparently these are very expensive. Does anyone have any suggestions? > > Rhonda Hartz > Technologist Supervisor > Anatomic Pathology Division > Saskatoon Health Region > (306) 655-8197 > rhonda.hartz@saskatoonhealthregion.ca > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1 From Laura.Miller <@t> leica-microsystems.com Sat Jul 17 16:02:54 2010 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Sat Jul 17 16:03:01 2010 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 07/16/2010 and will not return until 07/26/2010. I am on vacation with very limited access to email. I will return your mesage when I am back in the office on July 26th. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From trathborne <@t> somerset-healthcare.com Sun Jul 18 08:55:35 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Sun Jul 18 08:55:45 2010 Subject: [Histonet] Cytology 100 slide limit In-Reply-To: <73A7ED895EE0C24D9267ED814911DF1912B75647@exchange.cmc-nh.org> Message-ID: One other thing that must be considered are any cytotechs that have more than one job. You will need to know how many slides were screened at Job A so that the number is not exceeded at Job B. We had this issue when we were interviewing for a per diem cytotech to cover vacations - many could not guarantee that they would be able to screen the expected number of slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Feher, Stephen Sent: Friday, July 16, 2010 5:58 PM To: Rene J Buesa; Histonet; Victor Tobias Subject: RE: [Histonet] Cytology 100 slide limit Victor, Rene is correct in stating that CLIA allows Gyn Liquid Based Paps to be counted as 1/2 slide. It gets tricky when you start mixing 1/2 slide counts and full slide counts in making sure the techs do not exceed 100 slides (or in the case of LBP's 200 slides). If you get a lot of FNA's the counts can go up very quickly. Most LIS systems can prevent Techs from exceeding their limit. I would check again to see why your LIS is not capturing the NON-Gyn slides. Another CAP and CLIA requirement is that each 6 months, the cytotechs are supposed to have a Competency Assessment where the Medical Director signs off on the maximum number of slides each tech is qualified to screen. This is the number that most labs place in the LIS as the max that particular tech can review. What LIS system do you have? Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, July 16, 2010 5:21 PM To: Histonet; Victor Tobias Subject: Re: [Histonet] Cytology 100 slide limit Victor: As you wrote, either you?have to be?diligent in recording your work, or you will have to?modify the software of the LIS system to account correctly. On the other hand, if you are dealing with liquid base samples usually using a Thin-Prep Imaging System (TIS) and the sample covers one-half or less of the slide surface, CLIA88 has expanded the limit from 100 to 200 slides/day. Ren? J. --- On Fri, 7/16/10, Victor Tobias wrote: From: Victor Tobias Subject: [Histonet] Cytology 100 slide limit To: "Histonet" Date: Friday, July 16, 2010, 3:33 PM Our Cytology Supervisor was telling me about the 100 slide maximum that they can screen in a day. Our LIS is not capturing the NON-GYN slides being screened, so unless you are very diligent in recording the slides screened, you could go over the 100 limit. Our supervisor also believes the computer system should notify the user when the limit has been reached and prevent them from continuing. Is this a CAP requirement? How are you dealing with this problem or is it a problem for you? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From CrochiereSteve <@t> aol.com Sun Jul 18 09:38:03 2010 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Sun Jul 18 09:38:20 2010 Subject: [Histonet] Job Opening, Springfield MA Message-ID: <4ba95.3fbeb359.39746bcb@aol.com> We have an opening for a MOHS tech in our expanding laboratory. Responsibilities include freezing and sectioning of skin excisions . Staining, cover slipping, as well as equipment maintenance and record keeping. Previous experience is a plus, but will train the right candidate. HT(ASCP) or equivalent. Many exciting prospects are becoming available with the expansion of our laboratory service. Get in on the ground floor! The MOHS tech will be working one on one with a micrographic surgeon. Interested candidates should send resume to: Shannon Page, Clinical Manager New England Dermatology & Laser Center 3455 Main St. Springfield MA. 01107 _spage@nedlc.com_ (mailto:spage@nedlc.com) From Bill.Tench <@t> pph.org Sun Jul 18 15:25:53 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Sun Jul 18 15:26:05 2010 Subject: [Histonet] workload limits Message-ID: <2820431BF953BB4DA3E9E1A5882265FD027AA959@MAIL1.pph.local> I am a little concerned about some possible misunderstandings regarding slide limits, so below is a CAP article written on the topic that still applies. Understand that the elevated limits for image related instruments (which now include the ThinPrep Imager and the SurePath GS systems) have specific limits set for specific ways those instruments are used--and they are not the same for both instruments). Note well, liquid based paps do NOT count as 1/2 slides under any circumstances. You can only get to 200 slides per day if you are using the ThinPrep Imager and ONLY looking at fields of view. If you have to do a full manual review of the slide, you must reduce you limit accordingly. CAP Today August 2004 Special Section Q & A Q. We use a combination of manual and location-guided screening in my laboratory. How do I calculate cytotechnologist workload limits in this setting? A. Maximum workload limits should be calculated based on the following factors: Manual versus computer-assisted screening. CLIA regulations specify a 100-slide maximum limit for manual screening in 24 hours over a minimum eight-hour work day. This translates to a maximum screening rate of 12.5 slides per hour for manual screening. The Centers for Medicare and Medicaid Services recently set the maximum workload limit for location-guided screening at 200 slides per day. This limit is equivalent to 25 slides per hour for location-guided screening. All Pap tests count as whole slides. Slides from nongynecologic cases count as whole slides except for concentration and liquid-based techniques that confine the material to less than one-half of the slide surface, which may be counted as one-half slides. Quality control and five-year retrospective rescreens are also included in the workload accounting. Number of hours spent screening. The maximum workload limit should be prorated based on the total number of hours spent screening, using the 12.5 slides per hour maximum rate for manual screening and 25 slides per hour for computer-assisted screening. This time does not include computer data entry, reporting, and other duties. Cytotechnologist ability, experience, QC data, etc. It must be emphasized that the maximum workload limits are the maximum allowable by CLIA/CMS and are not to be used as a productivity goal. Each cytotechnologist's workload limit should be carefully determined and individualized based on expertise, time spent screening, and screening method(s) used. Here are a few scenarios to illustrate maximum workload limit calculations in different settings: Cytotechnologist A works in a high-volume laboratory and screens Paps using location-guided screening. She is a highly skilled cytotechnologist with 10 years of experience and has no other duties (no data entry or reporting, no answering phone). Her maximum workload limit is 200 slides per day over eight hours (25 slides/hour). This case scenario is a rare exception. Most cytotechnologists have other duties or have a lower personal workload limit set by the laboratory supervisor and medical director. Cytotechnologist B's laboratory just brought a computerized imaging system in house and is gradually making a transition to computer-assisted screening. She now screens about two hours per day using the computer-assisted device and three hours per day screening Paps manually. She spends the rest of her day doing clerical and cytopreparatory work. Her maximum workload allowed by CLIA is: (2 hrs x 25 slides/hr) + (3 hrs x 12.5 slides/hr)=87.5 slides Cytotechnologist C screens Paps every morning using location-guided screening and screens nongyns in the afternoon. Today he spent four hours screening Paps. Although the maximum limit by CLIA would be 25 x 4=100, his individual maximum screening limit is set a bit lower (20 slides per hour), so his maximum limit is 80 for four hours of screening. This afternoon is busy with several FNAs, each of about 20 slides. With four hours left of screening time at 12.5 slides per hour, he is allowed by CLIA to screen a maximum of 50 slides this afternoon. However, his individual workload limit is less, and he will be permitted to screen a maximum of 35 of the FNA slides, and other cytotechnologists will screen the rest. Although computer-assisted screening methods afford greater productivity, care should be taken to set reasonable maximum workload limits based on the ability of the individual cytotechnologist, to avoid compromising screening accuracy. Theresa M. Voytek, MD Department of Pathology Bill Tench Palomar Medical Center 555 E Valley Parkway Escondido, Ca 92025 Voice: 760-739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- From vhlwong <@t> yahoo.com Sun Jul 18 22:31:57 2010 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Sun Jul 18 22:32:00 2010 Subject: [Histonet] ki-67 staining Message-ID: <248554.40272.qm@web52706.mail.re2.yahoo.com> Dear all, ? I am working on imumunohistichemistry of ki-67 antibody (DAKO MIB-5, a monoclonal mouse anti-rat) on EDTA-decalcifed rat spine and femur.? I used DAKO's reagents such as Dual?enzyme block, antigen retrieval solution pH6.0, normal goat serum, anti-mouse Envision plus kit and the DAB kit.? I washed with TBS (prepared from Invitrogen's 1M Tris-HCl pH7.5 + NaCl to 50mM Tris.HCl pH 7.4, 150mM NaCl without pH adjustment).? I used 10% NBF fixed rat liver as a control.? However, none in the liver was stained and there were?non-specific staining in the samples.? Below is my protocol. ? 1.?????????????Dewax and rehydrate sections 2.?????????????a.?? Prepare DAKO antigen retrieval solution by diluting 1:10 with distilled water; b.????? preheating the solution by microwave (boil); c.?????? heat antigen retrieval solution in a copin jar with microwave; d.????? put slides to the solution and leave for 20 minutes?under low to medium microwave power. e.?????? Cool for 20 minutes 3.????????????????? Wash with TBS 4.????????????????? Quench sections in Dual Endogenous Enzyme Block for 10 minutes 5.????????????????? Rinse with TBS 6.????????????????? Blocking the slides with 5% goat serum for 20 mins 7.????????????????? Incubate with 1? antibody (1:25) in 5% goat serum for 1 hours RT 8.????????????????? wash in TBS @1min x 3 9.????????????????? Incubate with EnVision HRP-conjugated anti-mouse antibody for 30 mins 10.????????????? Wash in TBS @1min x 3 11.????????????? Add DAB (20ul into 1ml DAB+substrate buffer, mix immediately) to develop 12.????????????? Wash in water 13.????????????? Counterstain in haemotoxylin 14.????????????? Wash in running tap water 15.????????????? Dehydrate, clear and mount ? Could anyone help me?in troubleshooting which steps went wrong or giving suggestion on the staining protocol?? Any suggstion in the antigen retrieval procedures are also welcomed. ? Many thanks in advance. ? Best Regards, Victor From L.Moffat <@t> hrsu.mrc.ac.uk Mon Jul 19 06:53:57 2010 From: L.Moffat <@t> hrsu.mrc.ac.uk (Lindsey Moffat) Date: Mon Jul 19 06:55:17 2010 Subject: [Histonet] Apoptosis staining on mouse testis Message-ID: <39A5A5C9BAF7ED48A22E7AD7C4763638049CBE03A9@MAILSERV2.hrsu.mrc.ac.uk> Hello all, I am trying to stain for apoptosis on bouins fixed, paraffin embedded mouse testis. I have tried a cleaved caspase-3 anitbody, & TUNEL, with no success. Any suggestions would be much appreciated. Many thanks Lindsey From BenatM <@t> gosh.nhs.uk Mon Jul 19 07:32:08 2010 From: BenatM <@t> gosh.nhs.uk (Malika Benatti) Date: Mon Jul 19 07:32:44 2010 Subject: [Histonet] Lyve - 1 on Bond Max Message-ID: <4C4453D8.4626.0038.0@gosh.nhs.uk> ** Proprietary ** ** Reply Requested When Convenient ** Hi there, I was wondering if anyone on the histonet list use anti-mLYVE-1 antibody (purified in rat Monoclonal IgG 2A from R&D System) on the Bond Max. We have been trying out various titrations and pre-treatment without much success. All we seems to achieve is background staining with no real specificity at 1:100 H1(10) ; 1:100 H2(20) ;1:100 E2(10): 1:50 E1(30) ;1:50 H1(30); 1:50 H2(10) Any suggestion ? Regards Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* From andreahooper <@t> rocketmail.com Mon Jul 19 08:06:23 2010 From: andreahooper <@t> rocketmail.com (andreahooper@rocketmail.com) Date: Mon Jul 19 08:06:32 2010 Subject: [Histonet] human on human IHC In-Reply-To: <20100715092719.9e2d9aa830e8449a2412eb1e4f2f067e.64c033395c.wbe@email04.secureserver.net> References: <20100715092719.9e2d9aa830e8449a2412eb1e4f2f067e.64c033395c.wbe@email04.secureserver.net> Message-ID: <1658668231-1279544784-cardhu_decombobulator_blackberry.rim.net-110444085-@bda665.bisx.prod.on.blackberry> Along those same lines I have conjugated primaries to biotin and it works beautifully for human on human IHC. Another alternative if extra amplification is needed is to conjugating to FITC and then using a rabbit-anti-FITC followed by an anti-rabbit polymer. Just be aware with human on human controls are imperative as you may get binding to human Fc receptors. Andrea Hooper Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Sender: histonet-bounces@lists.utsouthwestern.edu Date: Thu, 15 Jul 2010 09:27:19 To: Kim Merriam Cc: Histonet Subject: RE: [Histonet] human on human IHC I am doing alot with this right now. The best thing I havefound to do is to conjugate your primary antibody with HRP, then just use the direct method (no secondary), and it turns out beautifully!! You can buy thekit to do this from Fisher. I can get the exact order number if you need it. Good Luck!! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] human on human IHC From: Kim Merriam <[1]kmerriam2003@yahoo.com> Date: Thu, July 15, 2010 8:00 am To: Histonet <[2]histonet@lists.utsouthwestern.edu> Hi, Does anyone know of a human-on-human IHC kit similar to the mouse-on-mouse kits that are available? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list [3]Histonet@lists.utsouthwestern.edu [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto://kmerriam2003@yahoo.com"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Mon Jul 19 08:53:46 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Jul 19 08:53:51 2010 Subject: [Histonet] human on human IHC Message-ID: <20100719065346.9e2d9aa830e8449a2412eb1e4f2f067e.034561c94b.wbe@email04.secureserver.net> Anything using FITC is pretty expensive. If you aren't want flourescence then why would you use this? Does this specific conj ugation eliminate the Fc binding? Conjugating to HRP doesn't seem to que Sarah Goebel, B.A., HT (ASCP) Histotechnician < XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: Re: [Histonet] human on human IHC From: [1]andreahooper@rocketm Date: Mon, July 19, 2010 6:06 am To: [2]sgoebel@xbiotech.com, "Ki oo.com> Cc: "Histonet" <[4]h Along those same lines I have conjugated primaries to biotin and it works b extra amplificati using a rabbit-anti-FITC fo Just be aware with human on human controls are imperative as you may get bi Andrea Hooper Sent from my Verizon Wireless BlackBerry -----Original Message----- From: <[5]sgoebel@xbiotech.com> Sender: [6]hist Date: Thu, 15 Jul 2010 09:27:19 To: Kim Merriam<[7]kmerriam2003 Cc: Histonet<[8]hist Subject: RE: [Histonet] human on human IHC I am doing alot with this right now. The best thing I havefound to do is to conjugate your primary antibody with HRP, then just use the direct method (no secondary), and it turns out beautifully!! You can buy thekit to do this from Fisher. I can get the exact order number if you need it. Good Luck!! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] human on human IHC From: Kim Merriam <[1][9]kme Date: Thu, July 15, 2010 8:00 am To: Histonet <[2][10]histonet@lists.utsouthwestern.edu> Hi, Does anyone know of a human-on-human IHC kit similar to the mouse-on-mouse kits that are available? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list [3][11]Histonet@lists [4][12]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"[13]mailto://kmerriam2003@ 2. 3D"[14]mailto://hi 3. 3D"[15]mailto://Hi 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list [16]Histonet@lists.utsou [17]http: References 1. 3D"mailto://andreahooper@rocketmail.com"/ 2. 3D"mailto://sgoebel@xbiotech.com"/ 3. 3D"mailto://kmerriam2003@yahoo.com"/ 4. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 5. 3D"mailto://sgoebel@xbiotech.com"/ 6. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 7. 3D"mailto://kmerriam2003@yahoo.com"/ 8. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 9. 3D"mailto://kmerriam2003@yahoo.com"/ 10. 3D"mailto://histonet@lists.utsouthwestern.ed=/ 11. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 12. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 13. 3D"mailto://kmerriam2003@yahoo.com"/ 14. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 15. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 16. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 17. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From adoughty <@t> lrri.org Mon Jul 19 09:29:19 2010 From: adoughty <@t> lrri.org (Doughty, Adele) Date: Mon Jul 19 09:29:26 2010 Subject: [Histonet] vanGieson-Trichrome staining protocol Message-ID: Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele From bhewlett <@t> cogeco.ca Mon Jul 19 09:37:35 2010 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Jul 19 09:37:45 2010 Subject: [Histonet] vanGieson-Trichrome staining protocol References: Message-ID: Adele, Since the vanGieson IS a trichrome stain, why would anyone wish to combine one trichrome with another? Bryan ----- Original Message ----- From: "Doughty, Adele" To: Sent: Monday, July 19, 2010 10:29 AM Subject: [Histonet] vanGieson-Trichrome staining protocol Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Mon Jul 19 10:30:24 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Jul 19 10:30:30 2010 Subject: [Histonet] Wax removal In-Reply-To: References: Message-ID: Hi, Thanks for the reminder. We purchased them from Lab Safety Supply. They are called Safety Treads, and they come in a variety of sizes and shapes. The one we have is 6 X 24 in (I guesstimated the size wrong earlier) and it's part number is 10766BR. Here is a link to the web page for them: http://www.labsafety.com/search/10766/24527943/?GoButton=Go I hope this helps, it has worked great for us! Oh and getting them to sweep more regularly helped too, we were having a lot of trouble with dust. Happy Monday, Amos On Mon, Jul 19, 2010 at 10:31 AM, Hartz, Rhonda SktnHR < Rhonda.Hartz@saskatoonhealthregion.ca> wrote: > I would appreciate that info. > > Thanks. > > > Rhonda Hartz > Technologist Supervisor > Anatomic Pathology Division > Saskatoon Health Region > (306) 655-8197 > rhonda.hartz@saskatoonhealthregion.ca > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos > Brooks > Sent: July 16, 2010 7:04 PM > To: Hartz, Rhonda SktnHR; histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wax removal > > Hi, > You can get abrasive pads that have adhesive on one side. They are > usually used on stairs. They are like 8 inch wide 3 foot long strips of > sandpaper that stick (really well) to the floor. I have them placed > about 1 per foot along main traffic areas. I don't recall off the top of > my head where we got them from, but if you'd like, I'll look it up on > Monday. > > Amos > > > Message: 6 > Date: Thu, 15 Jul 2010 10:42:26 -0600 > From: "Hartz, Rhonda SktnHR" > Subject: [Histonet] Wax removal > To: > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Hi everyone; > > This may seem like a insignificant question, but we consistently have > issues with injuries to our maintenance staff from cleaning the wax off > of our floors. Housekeeping has laid sticky layered mats all over our > floors (like large mouse traps), which they peel off layer by layer as > they become wax covered. Apparently these are very expensive. Does > anyone have any suggestions? > > Rhonda Hartz > Technologist Supervisor > Anatomic Pathology Division > Saskatoon Health Region > (306) 655-8197 > rhonda.hartz@saskatoonhealthregion.ca > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From renafail <@t> bellsouth.net Mon Jul 19 12:47:25 2010 From: renafail <@t> bellsouth.net (Rena Fail) Date: Mon Jul 19 12:47:30 2010 Subject: [Histonet] vanGieson-Trichrome staining protocol In-Reply-To: References: Message-ID: <857812.52206.qm@web180817.mail.gq1.yahoo.com> Are you perhaps refering to the Verhoff's Van Gieson, if you are trying to stain elastic then? a trichrome it can be done.? The silver should be cloudy like a slightly dirty window , not milky? generally if you add enough ammonia to the silver and sodium hydroxide mixture?to render it completely clear then you will probably have added too much ammonia. Rena Fail ----- Original Message ---- From: "Doughty, Adele" To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 10:29:19 AM Subject: [Histonet] vanGieson-Trichrome staining protocol Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Jul 19 13:27:22 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Jul 19 13:27:28 2010 Subject: [Histonet] vanGieson-Trichrome staining protocol In-Reply-To: <857812.52206.qm@web180817.mail.gq1.yahoo.com> Message-ID: <6D2EF89D2260494E8DFD56045967B949@lurie.northwestern.edu> And it looks so pretty (VVG/TRI)- especially on lung. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rena Fail Sent: Monday, July 19, 2010 12:47 PM To: Doughty, Adele; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] vanGieson-Trichrome staining protocol Are you perhaps refering to the Verhoff's Van Gieson, if you are trying to stain elastic then? a trichrome it can be done.? The silver should be cloudy like a slightly dirty window , not milky? generally if you add enough ammonia to the silver and sodium hydroxide mixture?to render it completely clear then you will probably have added too much ammonia. Rena Fail ----- Original Message ---- From: "Doughty, Adele" To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 10:29:19 AM Subject: [Histonet] vanGieson-Trichrome staining protocol Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bstephen <@t> fastmail.fm Mon Jul 19 20:19:27 2010 From: bstephen <@t> fastmail.fm (Birgitta Stephenson) Date: Mon Jul 19 20:19:30 2010 Subject: [Histonet] Toluidine Blue and staining archaeological residues. Message-ID: <1279588767.6984.1385694279@webmail.messagingengine.com> Hello Histonetters, I am working with archaeological residues lifted from Holocene grindstones. I was trying to find a stain that could differentiate between plant and animal tissue in one hit. I have been using buffered Toluidine Blue solutions however given that these are ancient residues which are lifted using 20ul of water, the subtle colour differences between blues, purples, and violets have not been useful. I was looking at the possibility of counterstaining the Toluidine Blue stained residues with say Phloroglucinol or IKI which would highlight the plant component of the residue and then see if what was left looked like collagen. Has anyone tried this type of counterstaining? OR does anyone know of a stain that would colour differently for plant and animals understanding that this is not a tissue section but microscopic residues in solution? Thanks Birgitta Stephenson The Research Microscopy Laboratory, University of Queensland. -- Birgitta Stephenson bstephen@fastmail.fm -- http://www.fastmail.fm - Access your email from home and the web From MW <@t> PersonifySearch.com Tue Jul 20 07:38:29 2010 From: MW <@t> PersonifySearch.com (Matthew Ward) Date: Tue Jul 20 07:38:33 2010 Subject: [Histonet] New Opportunities with a World Leader in IHC! In-Reply-To: <002401cb2357$e277a590$a766f0b0$@com> References: <002401cb2357$e277a590$a766f0b0$@com> Message-ID: <002d01cb2808$755ba1e0$6012e5a0$@com> Good Morning Everyone, We are currently searching for Histotech's who have a strong background in IHC that would be interested in breaking into a field support role with a World Leader in Manufacturing Immunohistochemistry equipment. The company is going through a large expansion and are actively seeking candidates for the following locations. MD/VA/DC Southeast Florida (Miami/ Ft. Lauderdale) These Positions Offer: - Outstanding Base Salary and Bonus! - Gold Standard Benefits including but not limited to Medical, Cell Phone, Laptop, Car Allowance, Expenses, 401k, Paid Vacation! - Opportunity for Career Advancement! If you are interested in learning more please contact me directly at 800.875.6188 ext. 103 or mw@personifysearch.com Matt Ward Account Executive Personify 201?Shannon?Oaks Circle,?Suite?101 Cary,?North Carolina?27511 (Tel) 800.875.6188 direct ext 103 (Fax)?919.460.0642 ?www.personifysearch.com From contact <@t> excaliburpathology.com Tue Jul 20 08:51:09 2010 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Jul 20 08:51:13 2010 Subject: [Histonet] Toluidine Blue and staining archaeological residues. In-Reply-To: <1279588767.6984.1385694279@webmail.messagingengine.com> References: <1279588767.6984.1385694279@webmail.messagingengine.com> Message-ID: <413106.57072.qm@web1106.biz.mail.sk1.yahoo.com> Starch (plants) + Iodine = Black solution. ________________________________ From: Birgitta Stephenson To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 8:19:27 PM Subject: [Histonet] Toluidine Blue and staining archaeological residues. Hello Histonetters, I am working with archaeological residues lifted from Holocene grindstones. I was trying to find a stain that could differentiate between plant and animal tissue in one hit. I have been using buffered Toluidine Blue solutions however given that these are ancient residues which are lifted using 20ul of water, the subtle colour differences between blues, purples, and violets have not been useful. I was looking at the possibility of counterstaining the Toluidine Blue stained residues with say Phloroglucinol or IKI which would highlight the plant component of the residue and then see if what was left looked like collagen.? Has anyone tried this type of counterstaining? OR does anyone know of a stain that would colour differently for plant and animals understanding that this is not a tissue section but microscopic residues in solution? Thanks Birgitta Stephenson The Research Microscopy Laboratory, University of Queensland. -- ? Birgitta Stephenson ? bstephen@fastmail.fm -- http://www.fastmail.fm - Access your email from home and the web _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Tue Jul 20 10:15:24 2010 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Tue Jul 20 10:15:31 2010 Subject: [Histonet] Paraffin in processor Message-ID: <3809C163DC1DA54AA534B3C7794D07B660AEF6EC36@ENHBGMBX01.PA.LCL> Greetings all, We ran into an issue with the level of our first paraffin being extremely low one day. We normally rotate paraffin at 300 blocks but sometimes this could take 4-5 weeks and 15-20 runs before we get to this magic number. My question is if we change to a number of runs to change/rotate reagents what would be the best number to choose. Five runs much like our cleaning reagents or can we stretch to 10 runs. The day our volume was low we had 16 runs documented. Thanks so much. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us From ebinder <@t> mmm.com Tue Jul 20 12:09:02 2010 From: ebinder <@t> mmm.com (ebinder@mmm.com) Date: Tue Jul 20 12:09:11 2010 Subject: [Histonet] Fluid for snap freezing (as in histobath fluid) Message-ID: I noticed that our Novec 7100 has been discussed many times on Histonet as an alternative to isopentane in Histobath and we've had a number of laboratories who have ordered our fluid for this purpose. I'd like to learn more about what works well, whether this is the best fluid we have to offer, how to understand this market. Is it just because it's nonflammable that it's of interest? Or what other characteristics are important? My division is primarily involved in Electronics Markets, but if this is a new use, I'd really like to help and hopefully find a way to make the product more accessible and make sure it works. I'm looking for up to 10 hospitals or labs who would be willing to participate in a formal evaluation (I would supply fluid). I believe I can share results on Histonet if it's of interest. Erin Binder (651) 733 3203 office (612) 290 6343 mobile Market Development Mgr Electronic Markets Materials Division From Margaret.Perry <@t> sdstate.edu Tue Jul 20 14:49:21 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Jul 20 14:49:28 2010 Subject: [Histonet] EXPOSE Message-ID: Has anyone tried the new polyvalent polymer detection "EXPOSE" from AbCam? What did you think of it? Margaret SDSU From cls71877 <@t> sbcglobal.net Tue Jul 20 16:03:59 2010 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Tue Jul 20 16:04:04 2010 Subject: [Histonet] Utilization of consultation services Message-ID: <84474.37770.qm@web81203.mail.mud.yahoo.com> Hello Histoland, I am currently researching the utilization of consultation services for our lab.? We are a physician owned lab and therefore only have one pathologist on site at a time.? Does anyone else track this type of information?? Would you be willing to share the percentage of cases sent out to cases read?? Any assistance is greatly appreciated. Thanks, Cristi From sfeher <@t> CMC-NH.ORG Wed Jul 21 08:03:16 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Jul 21 08:03:20 2010 Subject: [Histonet] Utilization of consultation services In-Reply-To: <84474.37770.qm@web81203.mail.mud.yahoo.com> References: <84474.37770.qm@web81203.mail.mud.yahoo.com> Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B756A0@exchange.cmc-nh.org> Christi, Much depends on the composition of your cases and what your pathologists feel comfortable signing out. Some labs look at those cases that can be signed out quickly or have relatively high profit margins to do in house. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Tuesday, July 20, 2010 5:04 PM To: Histo Net Subject: [Histonet] Utilization of consultation services Hello Histoland, I am currently researching the utilization of consultation services for our lab.? We are a physician owned lab and therefore only have one pathologist on site at a time.? Does anyone else track this type of information?? Would you be willing to share the percentage of cases sent out to cases read?? Any assistance is greatly appreciated. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KarynMyers <@t> texashealth.org Wed Jul 21 08:57:35 2010 From: KarynMyers <@t> texashealth.org (Myers, Karyn) Date: Wed Jul 21 08:57:52 2010 Subject: [Histonet] Cryostat Decontamination Message-ID: <5B2323705FAC1B44A185BF385B33195A0238DA084A@PHDEXMB01.txhealth.org> Hello, Our Hospital has several Frozen Section Rooms, each of which have 1 to 2 cryostats. The majority of our cryostats are the newer models, with the built in Ultraviolet Radiation Disinfection cycle (runs every afternoon.) However, we still have a couple of the older models that do not have an automatic disinfection cycle. What are other institutions currently doing to disinfect their cryostats. Any information/policy will be greatly appreciated, Karyn The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From plucas <@t> biopath.org Wed Jul 21 09:02:50 2010 From: plucas <@t> biopath.org (Paula Lucas) Date: Wed Jul 21 08:58:03 2010 Subject: [Histonet] Per Diem Histotech Needed (Orange County, California) Message-ID: <9F3863CC7A1C4C529FDB36AC74886B2E@biopath.local> Hello- We are located in Fountain Valley, California and we are seeking a per diem histotech to cover during vacation times or other times when the techs are scheduled off. We are seeking a technician who has experience in embedding and cutting surgical tissue cases. Coverage START time would be early morning hours starting between 4:30 am to 7 am, preferably 5 am start time but I can be somewhat flexible with that. The total hours in a day would be between 4 and 6 hours. If interested, please give me a summary of your work history. Please no recruiters/head hunters respond to my email, only qualified techs who are interested. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group From jcarpenter764 <@t> aol.com Wed Jul 21 09:09:50 2010 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Wed Jul 21 09:10:23 2010 Subject: [Histonet] Certification question???? Message-ID: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. From jcarpenter764 <@t> aol.com Wed Jul 21 09:09:50 2010 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Wed Jul 21 09:10:26 2010 Subject: [Histonet] Certification question???? Message-ID: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. From brinkerk <@t> musc.edu Wed Jul 21 09:21:44 2010 From: brinkerk <@t> musc.edu (Geils, Karen Brinker) Date: Wed Jul 21 09:21:46 2010 Subject: [Histonet] Certification question???? In-Reply-To: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> References: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> Message-ID: The ASCP site provides the requirements for certification. The exam is online and offered throughout the year. http://www.ascp.org/FunctionalNavigation/certification/GetCertified.aspx -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jcarpenter764@aol.com Sent: Wednesday, July 21, 2010 10:10 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brinkerk <@t> musc.edu Wed Jul 21 09:21:44 2010 From: brinkerk <@t> musc.edu (Geils, Karen Brinker) Date: Wed Jul 21 09:21:54 2010 Subject: [Histonet] Certification question???? In-Reply-To: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> References: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> Message-ID: The ASCP site provides the requirements for certification. The exam is online and offered throughout the year. http://www.ascp.org/FunctionalNavigation/certification/GetCertified.aspx -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jcarpenter764@aol.com Sent: Wednesday, July 21, 2010 10:10 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Wed Jul 21 10:35:42 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Jul 21 10:35:52 2010 Subject: [Histonet] decontamination of cryostat Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A546F@MAIL1.pph.local> I would suggest that if you are a CAP certified lab that you read the appropriate section in the current LAP standards list. The note associated with the standard is pretty specifiic. I believe that if you use the cryostat daily, you need to decontaminate once a week, but i don't have the standard available. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- From Valerie.Hannen <@t> parrishmed.com Wed Jul 21 10:43:18 2010 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Wed Jul 21 10:45:53 2010 Subject: [Histonet] Certification question???? References: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> Message-ID: <5680DA93771F0C48954CC8D38425E72401AD03CB@ISMAIL.parrishmed.local> The test is online...but you still have to go to a facility to take it...I believe you have to have a person who monitors the test, to ensure that no one cheats. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jcarpenter764@aol.com Sent: Wed 7/21/2010 10:09 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From Valerie.Hannen <@t> parrishmed.com Wed Jul 21 10:43:18 2010 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Wed Jul 21 10:45:55 2010 Subject: [Histonet] Certification question???? References: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> Message-ID: <5680DA93771F0C48954CC8D38425E72401AD03CB@ISMAIL.parrishmed.local> The test is online...but you still have to go to a facility to take it...I believe you have to have a person who monitors the test, to ensure that no one cheats. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jcarpenter764@aol.com Sent: Wed 7/21/2010 10:09 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From relia1 <@t> earthlink.net Wed Jul 21 10:49:07 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jul 21 10:49:12 2010 Subject: [Histonet] Histology Externships for New Grads. A message from Pam Barker at RELIA Message-ID: Hello Histonetters ! I hope you are having a great summer. Yesterday I visited Keiser University here in Orlando, FL. They are getting ready to graduate their first class of histotechnologists from their Histotechnology program. I met a lot of bright, interested and dedicated future histotechnologists. The reason I am telling you this is because as part of the program the students do an externship. Would you be interested in becoming an externship site? Here is the information on the program and contact information for the director of student services. Have a great day! Keiser University's Associate of Science degree in Histotechnology prepares students to perform laboratory studies of tissue. The program includes the study of cellular morphology, chemical composition and the functions of normal and abnormal tissue. Students study the composition of dyes and chemicals and gain a thorough understanding of tissue composition. Upon completion of their degree program, our students are prepared and eligible to sit for their HT (ASCP) license. Our students are required to complete two months of externship. If you are interested in becoming an externship site, please contact Nicole Goodman at ngoodman@keiseruniversity.edu or 407-273-5800. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From JEllin <@t> yumaregional.org Wed Jul 21 11:01:19 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Jul 21 11:01:03 2010 Subject: [Histonet] decontamination of cryostat In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A546F@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A546F@MAIL1.pph.local> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C64E2@EXCHANGECLUSTER.yumaregional.local> Bill and company This is a very difficult, there are times were decontamination is needed immediately depending on the outcome of the frozen or there is scheduled maintenance that needs to occur with the decontamination as well. The UV light is not sufficient enough to call this decontamination. CAP has stated that it needs a specific reagent used in this decontamination procedure. My Chair was upset by this statement from the College and called them to ask for clarification. The main purpose is documented and scheduled times of decontamination is what we received back from the College. Of course there are circumstances when a case presents itself, action needs to be taken and documented. I would say is look at creating a scheduled decontamination that is appropriate for you facility. We have elected for a monthly Decontamination of cryostats, this is based on usage and history. This is documented and the appropriate solution is used during decontamination. This is done during the normally work day since we have two cryostats. This usually takes 3 days to do right, but some facilities might not be able to do this. Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Wednesday, July 21, 2010 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decontamination of cryostat I would suggest that if you are a CAP certified lab that you read the appropriate section in the current LAP standards list. The note associated with the standard is pretty specifiic. I believe that if you use the cryostat daily, you need to decontaminate once a week, but i don't have the standard available. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From annigyg <@t> gmail.com Wed Jul 21 11:23:35 2010 From: annigyg <@t> gmail.com (annigyg@gmail.com) Date: Wed Jul 21 11:31:06 2010 Subject: [Histonet] decontamination of cryostat In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8021C64E2@EXCHANGECLUSTER.yumaregional.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A546F@MAIL1.pph.local><29BE166A2CF48D459853F8EC57CD37E8021C64E2@EXCHANGECLUSTER.yumaregional.local> Message-ID: <554950759-1279729487-cardhu_decombobulator_blackberry.rim.net-1087292003-@bda242.bisx.produk.on.blackberry> If I may ask, what do you contaminate with when doing a full meltdown defrost clean up after a suspect TB or hep B pos case? Formalin? Some proprietary decontaminant? AnnieinArabia Empower your Business with BlackBerry? and Mobile Solutions from Etisalat -----Original Message----- From: "Jesus Ellin" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 21 Jul 2010 09:01:19 To: Tench, Bill; Subject: RE: [Histonet] decontamination of cryostat Bill and company This is a very difficult, there are times were decontamination is needed immediately depending on the outcome of the frozen or there is scheduled maintenance that needs to occur with the decontamination as well. The UV light is not sufficient enough to call this decontamination. CAP has stated that it needs a specific reagent used in this decontamination procedure. My Chair was upset by this statement from the College and called them to ask for clarification. The main purpose is documented and scheduled times of decontamination is what we received back from the College. Of course there are circumstances when a case presents itself, action needs to be taken and documented. I would say is look at creating a scheduled decontamination that is appropriate for you facility. We have elected for a monthly Decontamination of cryostats, this is based on usage and history. This is documented and the appropriate solution is used during decontamination. This is done during the normally work day since we have two cryostats. This usually takes 3 days to do right, but some facilities might not be able to do this. Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Wednesday, July 21, 2010 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decontamination of cryostat I would suggest that if you are a CAP certified lab that you read the appropriate section in the current LAP standards list. The note associated with the standard is pretty specifiic. I believe that if you use the cryostat daily, you need to decontaminate once a week, but i don't have the standard available. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Elliott <@t> hli.ubc.ca Wed Jul 21 12:29:29 2010 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Wed Jul 21 12:31:40 2010 Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 24 In-Reply-To: <1C5C64C4.456@mail.mrl.ubc.ca> References: <1C5C64C4.456@mail.mrl.ubc.ca> Message-ID: <4C46CC09020000D60004C42E@mail.mrl.ubc.ca> Hi everyone. I was asked the following question: "I am just wondering if quantifying PAS staining (purple) is proportional to the amount of mucus produced from goblet cells?" Thought I would ask the experts their opinion. Thanks Mark in sunny, warm Vancouver BC ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From luke.perkocha <@t> ucsf.edu Wed Jul 21 12:37:26 2010 From: luke.perkocha <@t> ucsf.edu (Perkocha, Luke) Date: Wed Jul 21 12:51:41 2010 Subject: [Histonet] Vendors of Specimen Tracking Systems Message-ID: Dear Listservers, We're trying to compile a list of vendors of AP/histology specimen tracking to investigate. We have Cerner Co-path LIS, and they have their own product. The following are other vendors we've heard of - are their any others we should investigate? Which of these are "not ready for prime time" in your estimation? * Ventana * Dako * Omni Trax * Label Clinic * ID positive, from General Data * Brady Specimen Labeling * University of Washington system - ? awaiting commercialization Many thanks! Luke Perkocha, MD, MBA Associate Professor, Pathology and Dermatology Associate Director, Surgical Pathology University of California, San Francisco Office: 415 885-7254 From JEllin <@t> yumaregional.org Wed Jul 21 13:16:27 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Jul 21 13:16:11 2010 Subject: [Histonet] decontamination of cryostat In-Reply-To: <554950759-1279729487-cardhu_decombobulator_blackberry.rim.net-1087292003-@bda242.bisx.produk.on.blackberry> References: <2820431BF953BB4DA3E9E1A5882265FD034A546F@MAIL1.pph.local><29BE166A2CF48D459853F8EC57CD37E8021C64E2@EXCHANGECLUSTER.yumaregional.local> <554950759-1279729487-cardhu_decombobulator_blackberry.rim.net-1087292003-@bda242.bisx.produk.on.blackberry> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C64EE@EXCHANGECLUSTER.yumaregional.local> We use the Lysol Brand IC and Envirocide. We switch off every other month to prevent resistance to the cleaner that is being used within the instrument. Jesus Ellin Yuma Regional Medical Center 928-336-1743 -----Original Message----- From: annigyg@gmail.com [mailto:annigyg@gmail.com] Sent: Wednesday, July 21, 2010 9:24 AM To: Jesus Ellin; histonet-bounces@lists.utsouthwestern.edu; Tench, Bill; Histonet Subject: Re: [Histonet] decontamination of cryostat If I may ask, what do you contaminate with when doing a full meltdown defrost clean up after a suspect TB or hep B pos case? Formalin? Some proprietary decontaminant? AnnieinArabia Empower your Business with BlackBerry(r) and Mobile Solutions from Etisalat -----Original Message----- From: "Jesus Ellin" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 21 Jul 2010 09:01:19 To: Tench, Bill; Subject: RE: [Histonet] decontamination of cryostat Bill and company This is a very difficult, there are times were decontamination is needed immediately depending on the outcome of the frozen or there is scheduled maintenance that needs to occur with the decontamination as well. The UV light is not sufficient enough to call this decontamination. CAP has stated that it needs a specific reagent used in this decontamination procedure. My Chair was upset by this statement from the College and called them to ask for clarification. The main purpose is documented and scheduled times of decontamination is what we received back from the College. Of course there are circumstances when a case presents itself, action needs to be taken and documented. I would say is look at creating a scheduled decontamination that is appropriate for you facility. We have elected for a monthly Decontamination of cryostats, this is based on usage and history. This is documented and the appropriate solution is used during decontamination. This is done during the normally work day since we have two cryostats. This usually takes 3 days to do right, but some facilities might not be able to do this. Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Wednesday, July 21, 2010 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decontamination of cryostat I would suggest that if you are a CAP certified lab that you read the appropriate section in the current LAP standards list. The note associated with the standard is pretty specifiic. I believe that if you use the cryostat daily, you need to decontaminate once a week, but i don't have the standard available. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From malbenatti <@t> gmail.com Wed Jul 21 13:47:02 2010 From: malbenatti <@t> gmail.com (malbenatti@gmail.com) Date: Wed Jul 21 13:47:06 2010 Subject: [Histonet] Cryostat Decontamination Message-ID: Hy Karyrn. As my lab carry frozen section daily, the decontamination/fumigation of the cryostat on a daily basis is not practical as we do not have a back up cryostat. Also after every frozen section, we make sure that the Cryostat is get disinfected of any fresh tissue trimming with 70 % alcohol. Every friday night the cryostat get set-up for fumigation and get decontaminated weekly using Formaldehyde vapour/ then we neutralise them with ammonia, according to manufacturer recommendation (Thermo Scientific), though the fumigation will not activate until Sunday night so Week-end On-Call staff can carry urgent Frozen sections if needed. Each decontamination/fumigation cycle is then Log, for CPA purposes. Hope this helps Malika ---------- Forwarded message ---------- From: "Myers, Karyn" To: "'histonet@lists.utsouthwestern.edu'" Date: Wed, 21 Jul 2010 08:57:35 -0500 Subject: [Histonet] Cryostat Decontamination Hello, Our Hospital has several Frozen Section Rooms, each of which have 1 to 2 cryostats. The majority of our cryostats are the newer models, with the built in Ultraviolet Radiation Disinfection cycle (runs every afternoon.) However, we still have a couple of the older models that do not have an automatic disinfection cycle. What are other institutions currently doing to disinfect their cryostats. Any information/policy will be greatly appreciated, Karyn The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. -- " Smile .... it confuses people " From aaperghis <@t> uspath.com Wed Jul 21 14:20:36 2010 From: aaperghis <@t> uspath.com (Adrienne Aperghis Kavanagh) Date: Wed Jul 21 14:21:51 2010 Subject: [Histonet] ER/PR ON BENCHMARK XT In-Reply-To: <20100721170327.B13D138125C@mta1.brinkster.com> Message-ID: <9402243.239305.1279740036202.JavaMail.root@mail3d.brinkster.com> Hi All, Does anyone do ER/PR and/or CA15.3 on the Ventana Benchmark XT? If so, can you please tell me what protocol you are using? Thank you in advance, Adrienne Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 21, 2010 1:03:27 PM Subject: Histonet Digest, Vol 80, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Fluid for snap freezing (as in histobath fluid) (ebinder@mmm.com) 2. EXPOSE (Perry, Margaret) 3. Utilization of consultation services (Cristi stephenson) 4. RE: Utilization of consultation services (Feher, Stephen) 5. Cryostat Decontamination (Myers, Karyn) 6. Per Diem Histotech Needed (Orange County, California) (Paula Lucas) 7. Certification question???? (jcarpenter764@aol.com) 8. Certification question???? (jcarpenter764@aol.com) 9. RE: Certification question???? (Geils, Karen Brinker) 10. RE: Certification question???? (Geils, Karen Brinker) 11. decontamination of cryostat (Tench, Bill) 12. RE: Certification question???? (Hannen, Valerie) 13. RE: Certification question???? (Hannen, Valerie) 14. Histology Externships for New Grads. A message from Pam Barker at RELIA (Pam Barker) 15. RE: decontamination of cryostat (Jesus Ellin) 16. Re: decontamination of cryostat (annigyg@gmail.com) ---------------------------------------------------------------------- Message: 1 Date: Tue, 20 Jul 2010 12:09:02 -0500 From: ebinder@mmm.com Subject: [Histonet] Fluid for snap freezing (as in histobath fluid) To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I noticed that our Novec 7100 has been discussed many times on Histonet as an alternative to isopentane in Histobath and we've had a number of laboratories who have ordered our fluid for this purpose. I'd like to learn more about what works well, whether this is the best fluid we have to offer, how to understand this market. Is it just because it's nonflammable that it's of interest? Or what other characteristics are important? My division is primarily involved in Electronics Markets, but if this is a new use, I'd really like to help and hopefully find a way to make the product more accessible and make sure it works. I'm looking for up to 10 hospitals or labs who would be willing to participate in a formal evaluation (I would supply fluid). I believe I can share results on Histonet if it's of interest. Erin Binder (651) 733 3203 office (612) 290 6343 mobile Market Development Mgr Electronic Markets Materials Division ------------------------------ Message: 2 Date: Tue, 20 Jul 2010 14:49:21 -0500 From: "Perry, Margaret" Subject: [Histonet] EXPOSE To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Has anyone tried the new polyvalent polymer detection "EXPOSE" from AbCam? What did you think of it? Margaret SDSU ------------------------------ Message: 3 Date: Tue, 20 Jul 2010 14:03:59 -0700 (PDT) From: Cristi stephenson Subject: [Histonet] Utilization of consultation services To: Histo Net Message-ID: <84474.37770.qm@web81203.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Histoland, I am currently researching the utilization of consultation services for our lab.? We are a physician owned lab and therefore only have one pathologist on site at a time.? Does anyone else track this type of information?? Would you be willing to share the percentage of cases sent out to cases read?? Any assistance is greatly appreciated. Thanks, Cristi ------------------------------ Message: 4 Date: Wed, 21 Jul 2010 09:03:16 -0400 From: "Feher, Stephen" Subject: RE: [Histonet] Utilization of consultation services To: "Cristi stephenson" , "Histo Net" Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B756A0@exchange.cmc-nh.org> Content-Type: text/plain; charset="iso-8859-1" Christi, Much depends on the composition of your cases and what your pathologists feel comfortable signing out. Some labs look at those cases that can be signed out quickly or have relatively high profit margins to do in house. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Tuesday, July 20, 2010 5:04 PM To: Histo Net Subject: [Histonet] Utilization of consultation services Hello Histoland, I am currently researching the utilization of consultation services for our lab.? We are a physician owned lab and therefore only have one pathologist on site at a time.? Does anyone else track this type of information?? Would you be willing to share the percentage of cases sent out to cases read?? Any assistance is greatly appreciated. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 21 Jul 2010 08:57:35 -0500 From: "Myers, Karyn" Subject: [Histonet] Cryostat Decontamination To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <5B2323705FAC1B44A185BF385B33195A0238DA084A@PHDEXMB01.txhealth.org> Content-Type: text/plain; charset="us-ascii" Hello, Our Hospital has several Frozen Section Rooms, each of which have 1 to 2 cryostats. The majority of our cryostats are the newer models, with the built in Ultraviolet Radiation Disinfection cycle (runs every afternoon.) However, we still have a couple of the older models that do not have an automatic disinfection cycle. What are other institutions currently doing to disinfect their cryostats. Any information/policy will be greatly appreciated, Karyn The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. ------------------------------ Message: 6 Date: Wed, 21 Jul 2010 07:02:50 -0700 From: "Paula Lucas" Subject: [Histonet] Per Diem Histotech Needed (Orange County, California) To: Message-ID: <9F3863CC7A1C4C529FDB36AC74886B2E@biopath.local> Content-Type: text/plain; charset="us-ascii" Hello- We are located in Fountain Valley, California and we are seeking a per diem histotech to cover during vacation times or other times when the techs are scheduled off. We are seeking a technician who has experience in embedding and cutting surgical tissue cases. Coverage START time would be early morning hours starting between 4:30 am to 7 am, preferably 5 am start time but I can be somewhat flexible with that. The total hours in a day would be between 4 and 6 hours. If interested, please give me a summary of your work history. Please no recruiters/head hunters respond to my email, only qualified techs who are interested. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group ------------------------------ Message: 7 Date: Wed, 21 Jul 2010 10:09:50 -0400 From: jcarpenter764@aol.com Subject: [Histonet] Certification question???? To: histonet@pathology.swmed.edu, histonet@lists.utsouthwestern.edu Message-ID: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. ------------------------------ Message: 8 Date: Wed, 21 Jul 2010 10:09:50 -0400 From: jcarpenter764@aol.com Subject: [Histonet] Certification question???? To: histonet@pathology.swmed.edu, histonet@lists.utsouthwestern.edu Message-ID: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. ------------------------------ Message: 9 Date: Wed, 21 Jul 2010 10:21:44 -0400 From: "Geils, Karen Brinker" Subject: RE: [Histonet] Certification question???? To: "jcarpenter764@aol.com" , "histonet@pathology.swmed.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" The ASCP site provides the requirements for certification. The exam is online and offered throughout the year. http://www.ascp.org/FunctionalNavigation/certification/GetCertified.aspx -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jcarpenter764@aol.com Sent: Wednesday, July 21, 2010 10:10 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 21 Jul 2010 10:21:44 -0400 From: "Geils, Karen Brinker" Subject: RE: [Histonet] Certification question???? To: "jcarpenter764@aol.com" , "histonet@pathology.swmed.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" The ASCP site provides the requirements for certification. The exam is online and offered throughout the year. http://www.ascp.org/FunctionalNavigation/certification/GetCertified.aspx -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jcarpenter764@aol.com Sent: Wednesday, July 21, 2010 10:10 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 21 Jul 2010 08:35:42 -0700 From: "Tench, Bill" Subject: [Histonet] decontamination of cryostat To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A546F@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii I would suggest that if you are a CAP certified lab that you read the appropriate section in the current LAP standards list. The note associated with the standard is pretty specifiic. I believe that if you use the cryostat daily, you need to decontaminate once a week, but i don't have the standard available. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- ------------------------------ Message: 12 Date: Wed, 21 Jul 2010 11:43:18 -0400 From: "Hannen, Valerie" Subject: RE: [Histonet] Certification question???? To: , , Message-ID: <5680DA93771F0C48954CC8D38425E72401AD03CB@ISMAIL.parrishmed.local> Content-Type: text/plain; charset="UTF-8" The test is online...but you still have to go to a facility to take it...I believe you have to have a person who monitors the test, to ensure that no one cheats. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jcarpenter764@aol.com Sent: Wed 7/21/2010 10:09 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** ------------------------------ Message: 13 Date: Wed, 21 Jul 2010 11:43:18 -0400 From: "Hannen, Valerie" Subject: RE: [Histonet] Certification question???? To: , , Message-ID: <5680DA93771F0C48954CC8D38425E72401AD03CB@ISMAIL.parrishmed.local> Content-Type: text/plain; charset="UTF-8" The test is online...but you still have to go to a facility to take it...I believe you have to have a person who monitors the test, to ensure that no one cheats. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jcarpenter764@aol.com Sent: Wed 7/21/2010 10:09 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** ------------------------------ Message: 14 Date: Wed, 21 Jul 2010 11:49:07 -0400 From: "Pam Barker" Subject: [Histonet] Histology Externships for New Grads. A message from Pam Barker at RELIA To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters ! I hope you are having a great summer. Yesterday I visited Keiser University here in Orlando, FL. They are getting ready to graduate their first class of histotechnologists from their Histotechnology program. I met a lot of bright, interested and dedicated future histotechnologists. The reason I am telling you this is because as part of the program the students do an externship. Would you be interested in becoming an externship site? Here is the information on the program and contact information for the director of student services. Have a great day! Keiser University's Associate of Science degree in Histotechnology prepares students to perform laboratory studies of tissue. The program includes the study of cellular morphology, chemical composition and the functions of normal and abnormal tissue. Students study the composition of dyes and chemicals and gain a thorough understanding of tissue composition. Upon completion of their degree program, our students are prepared and eligible to sit for their HT (ASCP) license. Our students are required to complete two months of externship. If you are interested in becoming an externship site, please contact Nicole Goodman at ngoodman@keiseruniversity.edu or 407-273-5800. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 15 Date: Wed, 21 Jul 2010 09:01:19 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] decontamination of cryostat To: "Tench, Bill" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C64E2@EXCHANGECLUSTER.yumaregional.local> Content-Type: text/plain; charset="us-ascii" Bill and company This is a very difficult, there are times were decontamination is needed immediately depending on the outcome of the frozen or there is scheduled maintenance that needs to occur with the decontamination as well. The UV light is not sufficient enough to call this decontamination. CAP has stated that it needs a specific reagent used in this decontamination procedure. My Chair was upset by this statement from the College and called them to ask for clarification. The main purpose is documented and scheduled times of decontamination is what we received back from the College. Of course there are circumstances when a case presents itself, action needs to be taken and documented. I would say is look at creating a scheduled decontamination that is appropriate for you facility. We have elected for a monthly Decontamination of cryostats, this is based on usage and history. This is documented and the appropriate solution is used during decontamination. This is done during the normally work day since we have two cryostats. This usually takes 3 days to do right, but some facilities might not be able to do this. Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Wednesday, July 21, 2010 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decontamination of cryostat I would suggest that if you are a CAP certified lab that you read the appropriate section in the current LAP standards list. The note associated with the standard is pretty specifiic. I believe that if you use the cryostat daily, you need to decontaminate once a week, but i don't have the standard available. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ ------------------------------ Message: 16 Date: Wed, 21 Jul 2010 16:23:35 +0000 From: annigyg@gmail.com Subject: Re: [Histonet] decontamination of cryostat To: "Jesus Ellin" , histonet-bounces@lists.utsouthwestern.edu, "Tench, Bill" , "Histonet" Message-ID: <554950759-1279729487-cardhu_decombobulator_blackberry.rim.net-1087292003-@bda242.bisx.produk.on.blackberry> Content-Type: text/plain; charset="Windows-1252" If I may ask, what do you contaminate with when doing a full meltdown defrost clean up after a suspect TB or hep B pos case? Formalin? Some proprietary decontaminant? AnnieinArabia Empower your Business with BlackBerry? and Mobile Solutions from Etisalat -----Original Message----- From: "Jesus Ellin" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 21 Jul 2010 09:01:19 To: Tench, Bill; Subject: RE: [Histonet] decontamination of cryostat Bill and company This is a very difficult, there are times were decontamination is needed immediately depending on the outcome of the frozen or there is scheduled maintenance that needs to occur with the decontamination as well. The UV light is not sufficient enough to call this decontamination. CAP has stated that it needs a specific reagent used in this decontamination procedure. My Chair was upset by this statement from the College and called them to ask for clarification. The main purpose is documented and scheduled times of decontamination is what we received back from the College. Of course there are circumstances when a case presents itself, action needs to be taken and documented. I would say is look at creating a scheduled decontamination that is appropriate for you facility. We have elected for a monthly Decontamination of cryostats, this is based on usage and history. This is documented and the appropriate solution is used during decontamination. This is done during the normally work day since we have two cryostats. This usually takes 3 days to do right, but some facilities might not be able to do this. Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Wednesday, July 21, 2010 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decontamination of cryostat I would suggest that if you are a CAP certified lab that you read the appropriate section in the current LAP standards list. The note associated with the standard is pretty specifiic. I believe that if you use the cryostat daily, you need to decontaminate once a week, but i don't have the standard available. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 24 **************************************** From Vickroy.Jim <@t> mhsil.com Wed Jul 21 14:42:16 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Jul 21 14:42:21 2010 Subject: [Histonet] DISTILLED WATER FOR BUFFER FOR AUTOMATED STAINER Message-ID: <24A4826E8EF0964D86BC5317306F58A554D08E53EB@mmc-mail.ad.mhsil.com> WE ARE HAVING REAL ISSUES WITH THE PH OF THE DISTILLED WATER WE BUY COMMERCIALLY. WE PURCHASE A GENERAL DISTILLED WATER (HINKLEY) AND THE PH OF THE WATER IS CONSISTENTLY LOW 6.0 OR LESS. WE TRIED A NURSERY WATER (WITH A FEW MINERALS ADDED FOR TASTE) AND THE PH WAS 7.0 EXACTLY. HAS ANYBODY ELSE EXPERIENCED PROBLEMS WITH THE BUFFER PH BECAUSE OF DISTILLED WATER PH AND DOES ANYBODY HAVE ANY SUGGESTIONS? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Cathy.Crumpton <@t> tuality.org Wed Jul 21 14:46:38 2010 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Wed Jul 21 14:46:46 2010 Subject: [Histonet] Biocare P63 with Dako Flex detection Message-ID: Hi all, I have ran into a troubleshooting dilemma and was wonderi if anyone else could help. Is there anyone out there that uses the Biocare Medical P63 concentrated antibody with the Dako TRS and Flex detec to do an enhancer, etc). basal cells in prosta pH 9 retrieval (which dilution with the same protoco Thanks, < Cathy Crumpton HT(ASCP), Histology Lead Tuality Community Hosp Hillsboro, OR 97123 (503)681-1292 From cdbeads <@t> earthlink.net Wed Jul 21 15:56:23 2010 From: cdbeads <@t> earthlink.net (Daniel) Date: Wed Jul 21 15:56:25 2010 Subject: [Histonet] Grossing Sites Message-ID: <26315733.1279745783631.JavaMail.root@elwamui-little.atl.sa.earthlink.net> I was wondering how any of you handle grossing of complex specimens from multiple sites. Do you transport them all to one central grossing pool, do them loacally and transport cassettes to a main Histology lab or some mixture of both? Also, if you gross at a centralized location how do deal with pathologists wanting to see the specimen grossing and orientation? Are you recording video, streaming grossing via Skype or some other system, or none of the above? Dan From foreightl <@t> gmail.com Wed Jul 21 16:24:38 2010 From: foreightl <@t> gmail.com (Pat Laurie) Date: Wed Jul 21 16:24:42 2010 Subject: [Histonet] DISTILLED WATER FOR BUFFER FOR AUTOMATED STAINER In-Reply-To: <24A4826E8EF0964D86BC5317306F58A554D08E53EB@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A554D08E53EB@mmc-mail.ad.mhsil.com> Message-ID: We ran into this a while ago too. Our milipore water is consistently at least 1 pH unit lower than 7. Our chemical safety specialist checked this out and found out that when the water initially comes out of the tap it is approx. 100% pure. Since there are no ingredients in the water at all, there is nothing to act like a buffer which tap water has. The water will start to become acidified by being exposed to the CO2 in the air and form carbonic acid H2CO3 naturally CO2 + H2O [image: is in equilibrium with] H2CO3 which pushed the pH down. We haven't come up with a good solution other than pHing the buffer after it is made. Good luck. On Wed, Jul 21, 2010 at 12:42 PM, Vickroy, Jim wrote: > > WE ARE HAVING REAL ISSUES WITH THE PH OF THE DISTILLED WATER WE BUY > COMMERCIALLY. WE PURCHASE A GENERAL DISTILLED WATER (HINKLEY) AND THE PH > OF THE WATER IS CONSISTENTLY LOW 6.0 OR LESS. WE TRIED A NURSERY WATER > (WITH A FEW MINERALS ADDED FOR TASTE) AND THE PH WAS 7.0 EXACTLY. HAS > ANYBODY ELSE EXPERIENCED PROBLEMS WITH THE BUFFER PH BECAUSE OF DISTILLED > WATER PH AND DOES ANYBODY HAVE ANY SUGGESTIONS? > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com From marktarango <@t> gmail.com Wed Jul 21 16:28:09 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jul 21 16:28:14 2010 Subject: [Histonet] Biocare P63 with Dako Flex detection In-Reply-To: References: Message-ID: Hi Cathy, I'm using their p63, but on the Ventana XT with ultraview DAB detection. We use it at 1:200 and get a strong signal in both breast and prostate tissues. If you haven't tried staining various cases of prostate, I'd suggest it. Sometimes I'll get a prostate that stains the basal cells nicely and other times the signal is much weaker. It might have something to do with fixation in the resection specimens. If you can't get a strong signal in a prostate needle biopsy, then it's definately a staining issue and not the tissue. I got stuck trying to stain a bad prostate once and wouldn't want that to happen to you. Good Luck! Mark On Wed, Jul 21, 2010 at 12:46 PM, wrote: > > Hi all, I have ran into a troubleshooting dilemma and was wonderi ng > if anyone else could help. Is there anyone out there that uses the > Biocare Medical P63 concentrated antibody with the Dako TRS and Flex > detec tion kit? I am curious what dilution you use and if you have > to do an ything special for the P63 (extra long retrieval, DAB > enhancer, etc). I am having problems getting a strong signal for the > basal cells in prosta te. I am running it at 1:50 with 40 minutes in > pH 9 retrieval (which seems too long to me but does help). A > dilution with the same protoco l at 1:100 is way too light. > > Thanks, > < /DIV> > Cathy Crumpton HT(ASCP), Histology Lead > Tuality Community Hosp ital > Hillsboro, OR 97123 > (503)681-1292 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Marilyn.A.Weiss <@t> kp.org Wed Jul 21 18:02:41 2010 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Wed Jul 21 18:03:10 2010 Subject: [Histonet] I will be out of office beginning the afternoon of 6/3/2010 returning 3/24/2010 Message-ID: I will be out of the office starting 07/20/2010 and will not return until 07/26/2010. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. From AnthonyH <@t> chw.edu.au Wed Jul 21 18:26:53 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jul 21 18:27:06 2010 Subject: [Histonet] DISTILLED WATER FOR BUFFER FOR AUTOMATED STAINER In-Reply-To: <24A4826E8EF0964D86BC5317306F58A554D08E53EB@mmc-mail.ad.mhsil.com> Message-ID: James, If you are using the distilled water for buffering then the strong buffer salts (eg phosphate, TRIS etc) will result in a solution that should not be affected by atmospheric CO2. If you dilute the buffer for use (as is common) then be careful that you do not dilute out the buffering capacity of the solution. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, 22 July 2010 5:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DISTILLED WATER FOR BUFFER FOR AUTOMATED STAINER WE ARE HAVING REAL ISSUES WITH THE PH OF THE DISTILLED WATER WE BUY COMMERCIALLY. WE PURCHASE A GENERAL DISTILLED WATER (HINKLEY) AND THE PH OF THE WATER IS CONSISTENTLY LOW 6.0 OR LESS. WE TRIED A NURSERY WATER (WITH A FEW MINERALS ADDED FOR TASTE) AND THE PH WAS 7.0 EXACTLY. HAS ANYBODY ELSE EXPERIENCED PROBLEMS WITH THE BUFFER PH BECAUSE OF DISTILLED WATER PH AND DOES ANYBODY HAVE ANY SUGGESTIONS? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From raj <@t> bluemarble.net Wed Jul 21 18:34:42 2010 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Wed Jul 21 18:34:56 2010 Subject: [Histonet] Water Message-ID: <7E131EC1645D48769A9451F1DEBF2B92@CHURCH> Just a few questions. How many of you are using tap water in your stainers and what is the ph? I am interested in a Sakura prisma. Also I have a small stainer used for Moh's staining. I need a hood for it not a charcoal filter one. Your advice would be appreciated. Thanks From Tony_Reilly <@t> health.qld.gov.au Wed Jul 21 19:20:54 2010 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Wed Jul 21 19:21:26 2010 Subject: [Histonet] decontamination of cryostat In-Reply-To: <554950759-1279729487-cardhu_decombobulator_blackberry.rim.net-1087292003-@bda242.bisx.produk.on.blackberry> References: <2820431BF953BB4DA3E9E1A5882265FD034A546F@MAIL1.pph.local><29BE166A2CF48D459853F8EC57CD37E8021C64E2@EXCHANGECLUSTER.yumaregional.local> <554950759-1279729487-cardhu_decombobulator_blackberry.rim.net-1087292003-@bda242.bisx.produk.on.blackberry> Message-ID: <4C481B85.471C.0039.0@health.qld.gov.au> Hi Annie If you look up this article or many others like it on the net the simplest and most effective disinfectants are 70% alcohol or one of the aldehydes for most organisms remembering that you are only disinfecting a hard surface with no visible volume of liquid containing organisms. We are fortunate to have 2 Thermo cryostats which have an inbuilt decontamination system using concentrated formaldehyde which can be run overnight performing a defrost and decon and as the microtome is not in the refrigerated chamber there is no problem with ice build up in the moving parts of the microtome. American Society for Microbiology Antiseptics and Disinfectants: Activity, Action, and Resistance Gerald McDonnell1* and A. Denver Russell2 STERIS Corporation, St. Louis Operations, St. Louis, Missouri 63166,1 and Welsh School of Pharmacy, Cardiff University, Cardiff CF1 3XF, United Kingdom2 All the best Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From bstephen <@t> fastmail.fm Thu Jul 22 05:18:48 2010 From: bstephen <@t> fastmail.fm (Birgitta Stephenson) Date: Thu Jul 22 05:18:53 2010 Subject: [Histonet] Toluidine Blue and staining archaeological residues. Message-ID: <1279793928.4144.1386127357@webmail.messagingengine.com> Hello Paula, Thanks for the reply. We do use IKI regualarly for starch staining, however once the slide is stained it is difficult to counterstain to detect animal residues which might be present. The residues from grindstones have lost a lot of their microsopic diagnostic properties and we are hponing that staining the residues will help differentiate between intensive specialised seed grinders versus multi-purpose expedient grinders. I am finding that the Phloroglucinol stain is light sensitive and therefore not permanent which allows for some counterstaining but still the technique is a work in progress. Any more thoughts most welcome. Regards Birgitta Stephenson Research Microscopy Lab, University of Queensland On Tue, 20 Jul 2010 06:51:09 -0700 (PDT), "Paula Pierce" said: > Starch (plants) + Iodine = Black solution. > > > > > ________________________________ > From: Birgitta Stephenson > To: histonet@lists.utsouthwestern.edu > Sent: Mon, July 19, 2010 8:19:27 PM > Subject: [Histonet] Toluidine Blue and staining archaeological residues. > > Hello Histonetters, > > I am working with archaeological residues lifted from Holocene > grindstones. I was trying to find a stain that could differentiate > between plant and animal tissue in one hit. I have been using buffered > Toluidine Blue solutions however given that these are ancient residues > which are lifted using 20ul of water, the subtle colour differences > between blues, purples, and violets have not been useful. I was looking > at the possibility of counterstaining the Toluidine Blue stained > residues with say Phloroglucinol or IKI which would highlight the plant > component of the residue and then see if what was left looked like > collagen.? Has anyone tried this type of counterstaining? OR does anyone > know of a stain that would colour differently for plant and animals > understanding that this is not a tissue section but microscopic residues > in solution? > > Thanks > Birgitta Stephenson > The Research Microscopy Laboratory, University of Queensland. > -- > ? Birgitta Stephenson > ? bstephen@fastmail.fm > > -- > http://www.fastmail.fm - Access your email from home and the web > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Birgitta Stephenson bstephen@fastmail.fm -- http://www.fastmail.fm - Access your email from home and the web From bstephen <@t> fastmail.fm Thu Jul 22 05:19:05 2010 From: bstephen <@t> fastmail.fm (Birgitta Stephenson) Date: Thu Jul 22 05:19:16 2010 Subject: [Histonet] Re: test animal vs. plant Message-ID: <1279793945.4143.1386127399@webmail.messagingengine.com> Hello Tamara, Thanks for your reply. In the lab we have worked with the carbohydrate ideas, so travelling down the same path. With the grindstones we are looking for some tests that we can also carry out in the field as sometimes we have grinding hollows or stones in situ which can not be moved. This is why we thought simple visual staining and utilising a hand held digital microscope could be the answer. However the staining is not as straight forward as we'd hoped. I am thinking that if the Phloroglucinol is light sensitive then perhaps counterstaining with Toluidine Blue can highlight other areas. Problem is the residues are water lifted and drowning the slide with tap water (well drops of) means that the residue at times almost vanishes. Any more thoughts are most welcome. Thnaks again Birgitta Stephenson Research Microscopy Lab, University of Queensland On Tue, 20 Jul 2010 11:41:51 -0600, "Tamara A Howard" said: > I saw your post on the Histonet - it seems that there must > specific tests that could do what you need. I Googled the > word string: "test distinguish plant animal" & got the > following promising hit: > > http://www.hsc.csu.edu.au/chemistry/options/forensic/2964/ch992nov03.html > > My guess is that there would be more info from forensic > science sites; they must have listservers, too. > > It sounds like a very interesting project - I'm envious! > > Tamara > > *************************** > Tamara Howard > Cell Biology & Physiology > UNM-HSC > Albuquerque, NM > *************************** -- Birgitta Stephenson bstephen@fastmail.fm -- http://www.fastmail.fm - Same, same, but different... From LSebree <@t> uwhealth.org Thu Jul 22 08:20:58 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Jul 22 08:21:08 2010 Subject: [Histonet] ER/PR ON BENCHMARK XT In-Reply-To: <9402243.239305.1279740036202.JavaMail.root@mail3d.brinkster.com> References: <20100721170327.B13D138125C@mta1.brinkster.com> <9402243.239305.1279740036202.JavaMail.root@mail3d.brinkster.com> Message-ID: <8C023B4AB999614BA4791BAEB26E2738399F18@UWHC-MAIL01.uwhis.hosp.wisc.edu> Adrienne, ER: Leica (Vision Biosystems), clone 6F11, mild CC1, 48" @ 42 degrees C, AB block, Hem II / 8" PR: VMS, clone 1E2, standard CC1, 32" @ 42 degrees C, AB block, Hem II / 8" Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Aperghis Kavanagh Sent: Wednesday, July 21, 2010 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ER/PR ON BENCHMARK XT Hi All, Does anyone do ER/PR and/or CA15.3 on the Ventana Benchmark XT? If so, can you please tell me what protocol you are using? Thank you in advance, Adrienne Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 21, 2010 1:03:27 PM Subject: Histonet Digest, Vol 80, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Fluid for snap freezing (as in histobath fluid) (ebinder@mmm.com) 2. EXPOSE (Perry, Margaret) 3. Utilization of consultation services (Cristi stephenson) 4. RE: Utilization of consultation services (Feher, Stephen) 5. Cryostat Decontamination (Myers, Karyn) 6. Per Diem Histotech Needed (Orange County, California) (Paula Lucas) 7. Certification question???? (jcarpenter764@aol.com) 8. Certification question???? (jcarpenter764@aol.com) 9. RE: Certification question???? (Geils, Karen Brinker) 10. RE: Certification question???? (Geils, Karen Brinker) 11. decontamination of cryostat (Tench, Bill) 12. RE: Certification question???? (Hannen, Valerie) 13. RE: Certification question???? (Hannen, Valerie) 14. Histology Externships for New Grads. A message from Pam Barker at RELIA (Pam Barker) 15. RE: decontamination of cryostat (Jesus Ellin) 16. Re: decontamination of cryostat (annigyg@gmail.com) ---------------------------------------------------------------------- Message: 1 Date: Tue, 20 Jul 2010 12:09:02 -0500 From: ebinder@mmm.com Subject: [Histonet] Fluid for snap freezing (as in histobath fluid) To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I noticed that our Novec 7100 has been discussed many times on Histonet as an alternative to isopentane in Histobath and we've had a number of laboratories who have ordered our fluid for this purpose. I'd like to learn more about what works well, whether this is the best fluid we have to offer, how to understand this market. Is it just because it's nonflammable that it's of interest? Or what other characteristics are important? My division is primarily involved in Electronics Markets, but if this is a new use, I'd really like to help and hopefully find a way to make the product more accessible and make sure it works. I'm looking for up to 10 hospitals or labs who would be willing to participate in a formal evaluation (I would supply fluid). I believe I can share results on Histonet if it's of interest. Erin Binder (651) 733 3203 office (612) 290 6343 mobile Market Development Mgr Electronic Markets Materials Division ------------------------------ Message: 2 Date: Tue, 20 Jul 2010 14:49:21 -0500 From: "Perry, Margaret" Subject: [Histonet] EXPOSE To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Has anyone tried the new polyvalent polymer detection "EXPOSE" from AbCam? What did you think of it? Margaret SDSU ------------------------------ Message: 3 Date: Tue, 20 Jul 2010 14:03:59 -0700 (PDT) From: Cristi stephenson Subject: [Histonet] Utilization of consultation services To: Histo Net Message-ID: <84474.37770.qm@web81203.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Histoland, I am currently researching the utilization of consultation services for our lab. We are a physician owned lab and therefore only have one pathologist on site at a time. Does anyone else track this type of information? Would you be willing to share the percentage of cases sent out to cases read? Any assistance is greatly appreciated. Thanks, Cristi ------------------------------ Message: 4 Date: Wed, 21 Jul 2010 09:03:16 -0400 From: "Feher, Stephen" Subject: RE: [Histonet] Utilization of consultation services To: "Cristi stephenson" , "Histo Net" Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B756A0@exchange.cmc-nh.org> Content-Type: text/plain; charset="iso-8859-1" Christi, Much depends on the composition of your cases and what your pathologists feel comfortable signing out. Some labs look at those cases that can be signed out quickly or have relatively high profit margins to do in house. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Tuesday, July 20, 2010 5:04 PM To: Histo Net Subject: [Histonet] Utilization of consultation services Hello Histoland, I am currently researching the utilization of consultation services for our lab. We are a physician owned lab and therefore only have one pathologist on site at a time. Does anyone else track this type of information? Would you be willing to share the percentage of cases sent out to cases read? Any assistance is greatly appreciated. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 21 Jul 2010 08:57:35 -0500 From: "Myers, Karyn" Subject: [Histonet] Cryostat Decontamination To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <5B2323705FAC1B44A185BF385B33195A0238DA084A@PHDEXMB01.txhealth.org> Content-Type: text/plain; charset="us-ascii" Hello, Our Hospital has several Frozen Section Rooms, each of which have 1 to 2 cryostats. The majority of our cryostats are the newer models, with the built in Ultraviolet Radiation Disinfection cycle (runs every afternoon.) However, we still have a couple of the older models that do not have an automatic disinfection cycle. What are other institutions currently doing to disinfect their cryostats. Any information/policy will be greatly appreciated, Karyn The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. ------------------------------ Message: 6 Date: Wed, 21 Jul 2010 07:02:50 -0700 From: "Paula Lucas" Subject: [Histonet] Per Diem Histotech Needed (Orange County, California) To: Message-ID: <9F3863CC7A1C4C529FDB36AC74886B2E@biopath.local> Content-Type: text/plain; charset="us-ascii" Hello- We are located in Fountain Valley, California and we are seeking a per diem histotech to cover during vacation times or other times when the techs are scheduled off. We are seeking a technician who has experience in embedding and cutting surgical tissue cases. Coverage START time would be early morning hours starting between 4:30 am to 7 am, preferably 5 am start time but I can be somewhat flexible with that. The total hours in a day would be between 4 and 6 hours. If interested, please give me a summary of your work history. Please no recruiters/head hunters respond to my email, only qualified techs who are interested. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group ------------------------------ Message: 7 Date: Wed, 21 Jul 2010 10:09:50 -0400 From: jcarpenter764@aol.com Subject: [Histonet] Certification question???? To: histonet@pathology.swmed.edu, histonet@lists.utsouthwestern.edu Message-ID: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. ------------------------------ Message: 8 Date: Wed, 21 Jul 2010 10:09:50 -0400 From: jcarpenter764@aol.com Subject: [Histonet] Certification question???? To: histonet@pathology.swmed.edu, histonet@lists.utsouthwestern.edu Message-ID: <8CCF6F5849D49AA-5AC-9DF4@webmail-d061.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. ------------------------------ Message: 9 Date: Wed, 21 Jul 2010 10:21:44 -0400 From: "Geils, Karen Brinker" Subject: RE: [Histonet] Certification question???? To: "jcarpenter764@aol.com" , "histonet@pathology.swmed.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" The ASCP site provides the requirements for certification. The exam is online and offered throughout the year. http://www.ascp.org/FunctionalNavigation/certification/GetCertified.aspx -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jcarpenter764@aol.com Sent: Wednesday, July 21, 2010 10:10 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 21 Jul 2010 10:21:44 -0400 From: "Geils, Karen Brinker" Subject: RE: [Histonet] Certification question???? To: "jcarpenter764@aol.com" , "histonet@pathology.swmed.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" The ASCP site provides the requirements for certification. The exam is online and offered throughout the year. http://www.ascp.org/FunctionalNavigation/certification/GetCertified.aspx -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jcarpenter764@aol.com Sent: Wednesday, July 21, 2010 10:10 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 21 Jul 2010 08:35:42 -0700 From: "Tench, Bill" Subject: [Histonet] decontamination of cryostat To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A546F@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii I would suggest that if you are a CAP certified lab that you read the appropriate section in the current LAP standards list. The note associated with the standard is pretty specifiic. I believe that if you use the cryostat daily, you need to decontaminate once a week, but i don't have the standard available. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- ------------------------------ Message: 12 Date: Wed, 21 Jul 2010 11:43:18 -0400 From: "Hannen, Valerie" Subject: RE: [Histonet] Certification question???? To: , , Message-ID: <5680DA93771F0C48954CC8D38425E72401AD03CB@ISMAIL.parrishmed.local> Content-Type: text/plain; charset="UTF-8" The test is online...but you still have to go to a facility to take it...I believe you have to have a person who monitors the test, to ensure that no one cheats. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jcarpenter764@aol.com Sent: Wed 7/21/2010 10:09 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** ------------------------------ Message: 13 Date: Wed, 21 Jul 2010 11:43:18 -0400 From: "Hannen, Valerie" Subject: RE: [Histonet] Certification question???? To: , , Message-ID: <5680DA93771F0C48954CC8D38425E72401AD03CB@ISMAIL.parrishmed.local> Content-Type: text/plain; charset="UTF-8" The test is online...but you still have to go to a facility to take it...I believe you have to have a person who monitors the test, to ensure that no one cheats. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jcarpenter764@aol.com Sent: Wed 7/21/2010 10:09 AM To: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Certification question???? Hey All, Wanted to see what the requirements are for getting certified. Does anyone know if the exam is online or do you still have to go to a facility to take it....all advice will be great! Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** ------------------------------ Message: 14 Date: Wed, 21 Jul 2010 11:49:07 -0400 From: "Pam Barker" Subject: [Histonet] Histology Externships for New Grads. A message from Pam Barker at RELIA To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters ! I hope you are having a great summer. Yesterday I visited Keiser University here in Orlando, FL. They are getting ready to graduate their first class of histotechnologists from their Histotechnology program. I met a lot of bright, interested and dedicated future histotechnologists. The reason I am telling you this is because as part of the program the students do an externship. Would you be interested in becoming an externship site? Here is the information on the program and contact information for the director of student services. Have a great day! Keiser University's Associate of Science degree in Histotechnology prepares students to perform laboratory studies of tissue. The program includes the study of cellular morphology, chemical composition and the functions of normal and abnormal tissue. Students study the composition of dyes and chemicals and gain a thorough understanding of tissue composition. Upon completion of their degree program, our students are prepared and eligible to sit for their HT (ASCP) license. Our students are required to complete two months of externship. If you are interested in becoming an externship site, please contact Nicole Goodman at ngoodman@keiseruniversity.edu or 407-273-5800. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 15 Date: Wed, 21 Jul 2010 09:01:19 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] decontamination of cryostat To: "Tench, Bill" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C64E2@EXCHANGECLUSTER.yumaregional.l ocal> Content-Type: text/plain; charset="us-ascii" Bill and company This is a very difficult, there are times were decontamination is needed immediately depending on the outcome of the frozen or there is scheduled maintenance that needs to occur with the decontamination as well. The UV light is not sufficient enough to call this decontamination. CAP has stated that it needs a specific reagent used in this decontamination procedure. My Chair was upset by this statement from the College and called them to ask for clarification. The main purpose is documented and scheduled times of decontamination is what we received back from the College. Of course there are circumstances when a case presents itself, action needs to be taken and documented. I would say is look at creating a scheduled decontamination that is appropriate for you facility. We have elected for a monthly Decontamination of cryostats, this is based on usage and history. This is documented and the appropriate solution is used during decontamination. This is done during the normally work day since we have two cryostats. This usually takes 3 days to do right, but some facilities might not be able to do this. Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Wednesday, July 21, 2010 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decontamination of cryostat I would suggest that if you are a CAP certified lab that you read the appropriate section in the current LAP standards list. The note associated with the standard is pretty specifiic. I believe that if you use the cryostat daily, you need to decontaminate once a week, but i don't have the standard available. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ ------------------------------ Message: 16 Date: Wed, 21 Jul 2010 16:23:35 +0000 From: annigyg@gmail.com Subject: Re: [Histonet] decontamination of cryostat To: "Jesus Ellin" , histonet-bounces@lists.utsouthwestern.edu, "Tench, Bill" , "Histonet" Message-ID: <554950759-1279729487-cardhu_decombobulator_blackberry.rim.net-108729200 3-@bda242.bisx.produk.on.blackberry> Content-Type: text/plain; charset="Windows-1252" If I may ask, what do you contaminate with when doing a full meltdown defrost clean up after a suspect TB or hep B pos case? Formalin? Some proprietary decontaminant? AnnieinArabia Empower your Business with BlackBerry and Mobile Solutions from Etisalat -----Original Message----- From: "Jesus Ellin" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 21 Jul 2010 09:01:19 To: Tench, Bill; Subject: RE: [Histonet] decontamination of cryostat Bill and company This is a very difficult, there are times were decontamination is needed immediately depending on the outcome of the frozen or there is scheduled maintenance that needs to occur with the decontamination as well. The UV light is not sufficient enough to call this decontamination. CAP has stated that it needs a specific reagent used in this decontamination procedure. My Chair was upset by this statement from the College and called them to ask for clarification. The main purpose is documented and scheduled times of decontamination is what we received back from the College. Of course there are circumstances when a case presents itself, action needs to be taken and documented. I would say is look at creating a scheduled decontamination that is appropriate for you facility. We have elected for a monthly Decontamination of cryostats, this is based on usage and history. This is documented and the appropriate solution is used during decontamination. This is done during the normally work day since we have two cryostats. This usually takes 3 days to do right, but some facilities might not be able to do this. Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Wednesday, July 21, 2010 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decontamination of cryostat I would suggest that if you are a CAP certified lab that you read the appropriate section in the current LAP standards list. The note associated with the standard is pretty specifiic. I believe that if you use the cryostat daily, you need to decontaminate once a week, but i don't have the standard available. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 24 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Jul 22 10:01:15 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jul 22 10:01:27 2010 Subject: [Histonet] HPV testing Message-ID: <4C4824FA.7400.0077.1@harthosp.org> What are labs doing for HPV testing on head and neck cancers when a request is received from a clinician? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From victor <@t> pathology.washington.edu Thu Jul 22 10:13:24 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Jul 22 10:13:31 2010 Subject: [Histonet] OmniTrax Message-ID: <4C486014.6000407@pathology.washington.edu> Good morning all, Just to clarify Dr. Perkocha's request on tracking programs. OmniTrax was developed by the Pathology Dept. at University of Washington Medical Center. For more information please go to http://pathwaypathologyconsultants.com/ Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From gprogers48 <@t> comcast.net Thu Jul 22 10:17:28 2010 From: gprogers48 <@t> comcast.net (Gary P Rogers) Date: Thu Jul 22 10:17:33 2010 Subject: [Histonet] Surplus Equipment Message-ID: <003601cb29b0$ff9d4170$fed7c450$@net> Good Morning Histonetters! I would like to speak to anyone that might have any surplus Histology equipment. I am specifically interested in the following items: 1. Leica XL Autostainer 2. Sakura Tissue Processors-"E" Series or Newer 3. Sakura SCA Tape Coverslippers 4. Leica Microtomes 5. Leica Cryostats Thanks in advance for your response Gary Rogers 484-886-6870 gary@usedhistologyequipment.com From Bill.Tench <@t> pph.org Thu Jul 22 10:18:27 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Thu Jul 22 10:18:39 2010 Subject: [Histonet] hpv testing on head and neck ca's Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5480@MAIL1.pph.local> We do p16 on squamous ca of the head and neck region routinely Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- From cbarone <@t> NEMOURS.ORG Thu Jul 22 12:21:26 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Thu Jul 22 12:21:31 2010 Subject: [Histonet] Laboratory Labeling Systems... Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7E87@wlmmsx01.nemours.org> Histonetters....What are your thoughts: We are getting ready to purchase a labeling system for our Core Facility. We have limited $'s and have demo'ed three systems. We have many different projects,with varying numbers of samples, blocks and slides. We would like to cover the widest amount of needs, with the system....not just cassettes and slides, but samples in freezers to antibodies we have alloquotted. We are about to purchase a two-D bar-coding system that can label everything we need and work with our present EXCEL data bases. We are leaning toward a Brady System, where we can purchase individually the parts we need for labeling cassettes to eppy's and we especially like that they... not only have a label that can be attached to frozen vials... but that there are several types of scanners available for the different stations in our lab. This system meets our CER requirements and is up-gradable for adding more stations if needed at alater time. Sounds too good to be true....so that is where you all come in. Is anyone using a Brady Labeling System? Good? Bad? Problems? What are your thoughts? Thanks for the impromptu survey. We plan to order in August, if there are no "big" issues, with those that use it. We demo's the labels and are happy with them. We are going alpha numeric/2-D...3 lines of text, is needed for our research work. Thanks for your thoughts. C. Barone Director Histotechnology Core Lab cbarone@nemours.org From srodriguez <@t> phenopath.com Thu Jul 22 12:44:10 2010 From: srodriguez <@t> phenopath.com (Stephanie Rodriguez) Date: Thu Jul 22 12:44:24 2010 Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 26 Message-ID: As do we. Stephanie Rodriguez, HTL(ASCP), QIHC Senior Molecular Technologist-FISH IHC Technologist III Phenopath Laboratories Seattle, WA (206) 374-9000 On 7/22/10 10:04 AM, "histonet-request@lists.utsouthwestern.edu" wrote: > > Message: 4 > Date: Thu, 22 Jul 2010 08:18:27 -0700 > From: "Tench, Bill" > Subject: [Histonet] hpv testing on head and neck ca's > To: histonet@lists.utsouthwestern.edu > Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5480@MAIL1.pph.local> > Content-Type: text/plain; charset=us-ascii > > We do p16 on squamous ca of the head and neck region routinely > > Bill Tench > Associate Dir. Laboratory Services > Chief, Cytology Services > Palomar Medical Center > 555 E. Valley Parkway > Escondido, California 92025 > Bill.Tench@pph.org > Voice: 760- 739-3037 > Fax: 760-739-2604 This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From kris <@t> idxpathology.com Thu Jul 22 12:43:52 2010 From: kris <@t> idxpathology.com (Scherer, Kris) Date: Thu Jul 22 12:44:28 2010 Subject: [Histonet] Histology metrics Message-ID: <0C15E5FC826B334BA019E489846D02357E06C23C55@RTWECS03.lca.labcorp.com> We are in need of a metrics for use in requesting an additional histotech. Does anyone have a system in place for presenting the need for an additional FTE, based on volume, worklow, vacations, etc? ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Jul 22 12:47:54 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Jul 22 12:48:56 2010 Subject: [Histonet] HPV testing In-Reply-To: <4C4824FA.7400.0077.1@harthosp.org> References: <4C4824FA.7400.0077.1@harthosp.org> Message-ID: Hi Dr. Cartun, We are running the HPV Genepoint test from Dako (in situ test). Along with the EGFR IHC. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Thursday, July 22, 2010 11:01 AM To: Histonet Subject: [Histonet] HPV testing What are labs doing for HPV testing on head and neck cancers when a request is received from a clinician? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Thu Jul 22 14:10:24 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Thu Jul 22 14:10:18 2010 Subject: [Histonet] Biocare P63 with Dako Flex detection In-Reply-To: References: Message-ID: <000001cb29d1$89f157b0$9dd40710$@com> Cathy Have you tried adding an EnVision FLEX mouse linker to your protocol. Sometimes that will work. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy.Crumpton@tuality.org Sent: Wednesday, July 21, 2010 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare P63 with Dako Flex detection Hi all, I have ran into a troubleshooting dilemma and was wonderi=g if anyone else could help. Is there anyone out there that uses the Biocare Medical P63 concentrated antibody with the Dako TRS and Flex detec=tion kit? I am curious what dilution you use and if you have to do an=ything special for the P63 (extra long retrieval, DAB enhancer, etc). = am having problems getting a strong signal for the basal cells in prosta=e. I am running it at 1:50 with 40 minutes in pH 9 retrieval (which =seems too long to me but does help). A dilution with the same protoco= at 1:100 is way too light. Thanks, <=DIV> Cathy Crumpton HT(ASCP), Histology Lead Tuality Community Hosp=tal Hillsboro, OR 97123 (503)681-1292 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jbirkner <@t> colabserv.com Thu Jul 22 14:11:18 2010 From: jbirkner <@t> colabserv.com (Jeff Birkner) Date: Thu Jul 22 14:11:29 2010 Subject: [Histonet] biohazard bags Message-ID: We are looking into our current use of biohazard bags. How many of you are currently re-using any bags for specimen transport? These would only be bags were the actual specimen was inside a secondary container and thus never directly came into contact with the bags. Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. From Stacy_McLaughlin <@t> cooley-dickinson.org Thu Jul 22 14:38:01 2010 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Thu Jul 22 14:38:10 2010 Subject: [Histonet] biohazard bags In-Reply-To: Message-ID: Re-use of specimen transport bio-bags is a big NO-NO in my laboratory. You run the risk of accidentally using one that is contaminated. (This actually happened, and it was not a pretty sight.) You can't guarantee that the outside of that specimen container in the bag is not contaminated, even though it looks clean. You don't know the practices of the person that collected it. This could be a big liability and the possible consequences just aren't worth it. Just my 2 cents. Stacy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jeff Birkner Sent: Thursday, July 22, 2010 3:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] biohazard bags We are looking into our current use of biohazard bags. How many of you are currently re-using any bags for specimen transport? These would only be bags were the actual specimen was inside a secondary container and thus never directly came into contact with the bags. Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Thu Jul 22 14:42:34 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Jul 22 14:42:46 2010 Subject: [Histonet] biohazard bags In-Reply-To: Message-ID: We were doing just that and our Infection Control Nurse has banded this practice. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Jeff Birkner" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/22/2010 03:11 PM To cc Subject [Histonet] biohazard bags We are looking into our current use of biohazard bags. How many of you are currently re-using any bags for specimen transport? These would only be bags were the actual specimen was inside a secondary container and thus never directly came into contact with the bags. Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From kgrobert <@t> rci.rutgers.edu Thu Jul 22 15:12:56 2010 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Thu Jul 22 15:13:00 2010 Subject: [Histonet] Sectioning problem Message-ID: <8b576f6ad1bfefb4659958e4c7c10cee.squirrel@webmail.rci.rutgers.edu> I am working on an old microtome, a Reichert Histostat with a Tissue-Tek II microtome chuck adapter, and the last time I had this machine cleaned and rehabbed by my repairman, he said that the springs were shot on the chuck adapter, resulting in the windowblind chatter that I was getting in my sections. Unfortunately he did not have a replacement, Sakura doesn't make or sell them anymore, and the one source for replacement adapters I did find online sold me a part that had the same problem-shot springs (I assume this, as I was getting the same chatter with the "new" part). I have Googled, searched on eBay, checked online used scientific/medical equipment suppliers to no avail...but I will keep looking in case something comes up. So I guess my question is...would one of the newer cassette holders fit in my microtomes? In addition to the Reichert mentioned above, I also have an American Optical 860. Or is it possible to figure out how to replace the springs in the thing? (I have half a mind to wander over to the mechanical engineering dept. here to ask that question, but I figured I'd start here on Histonet.) I would really hate to have to replace the microtomes just because I can't get a cassette holder for them. Thanks for all your help, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 From llewllew <@t> shaw.ca Thu Jul 22 16:02:24 2010 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Jul 22 16:02:40 2010 Subject: [Histonet] Sectioning problem In-Reply-To: <8b576f6ad1bfefb4659958e4c7c10cee.squirrel@webmail.rci.rutgers.edu> References: <8b576f6ad1bfefb4659958e4c7c10cee.squirrel@webmail.rci.rutgers.edu> Message-ID: <9CAE5B0417C54C3D9F56AB57B40271D0@BryanPC> I had spring problems on the block holder of a microtome many years ago. I solved it by getting a local engineering shop to make me some new springs using the old springs as a template. It worked fine, and was cheap, too. Bryan Llewellyn ----- Original Message ----- From: To: "histonet" Sent: Thursday, July 22, 2010 1:12 PM Subject: [Histonet] Sectioning problem I am working on an old microtome, a Reichert Histostat with a Tissue-Tek II microtome chuck adapter, and the last time I had this machine cleaned and rehabbed by my repairman, he said that the springs were shot on the chuck adapter, resulting in the windowblind chatter that I was getting in my sections. Unfortunately he did not have a replacement, Sakura doesn't make or sell them anymore, and the one source for replacement adapters I did find online sold me a part that had the same problem-shot springs (I assume this, as I was getting the same chatter with the "new" part). I have Googled, searched on eBay, checked online used scientific/medical equipment suppliers to no avail...but I will keep looking in case something comes up. So I guess my question is...would one of the newer cassette holders fit in my microtomes? In addition to the Reichert mentioned above, I also have an American Optical 860. Or is it possible to figure out how to replace the springs in the thing? (I have half a mind to wander over to the mechanical engineering dept. here to ask that question, but I figured I'd start here on Histonet.) I would really hate to have to replace the microtomes just because I can't get a cassette holder for them. Thanks for all your help, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Jul 22 17:40:45 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Jul 22 17:40:57 2010 Subject: [Histonet] Staining dish for vertical 30-slide rack Message-ID: <1AAF670737F193429070841C6B2ADD4C023D27BD4D@EXMBMCB15.ucsfmedicalcenter.org> Does anyone know of a plastic, inexpensive staining dish for the Richard-Allan or Raymond Lamb 30-slide vertical rack. Something similar to the tissue tek plastic stain dish. The Rack is 11cm / 4 3/8" long Tim Morken Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.morken@ucsfmedctr.org From trathborne <@t> somerset-healthcare.com Fri Jul 23 07:04:48 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Jul 23 07:04:55 2010 Subject: [Histonet] biohazard bags In-Reply-To: Message-ID: I agree. We never re-use them at my facility. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Stacy McLaughlin Sent: Thursday, July 22, 2010 3:38 PM To: Jeff Birkner; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] biohazard bags Re-use of specimen transport bio-bags is a big NO-NO in my laboratory. You run the risk of accidentally using one that is contaminated. (This actually happened, and it was not a pretty sight.) You can't guarantee that the outside of that specimen container in the bag is not contaminated, even though it looks clean. You don't know the practices of the person that collected it. This could be a big liability and the possible consequences just aren't worth it. Just my 2 cents. Stacy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jeff Birkner Sent: Thursday, July 22, 2010 3:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] biohazard bags We are looking into our current use of biohazard bags. How many of you are currently re-using any bags for specimen transport? These would only be bags were the actual specimen was inside a secondary container and thus never directly came into contact with the bags. Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From mchelle252002 <@t> yahoo.com Fri Jul 23 09:15:58 2010 From: mchelle252002 <@t> yahoo.com (Michelle Mathews) Date: Fri Jul 23 09:16:01 2010 Subject: [Histonet] p-TBK-1 Ab Message-ID: <510051.54589.qm@web53308.mail.re2.yahoo.com> Good morning everyone, I work in a research lab and am trying to optimize a p-TBK-1 antibody; I am running this work-up on a BondIII and a BondMax with a Dako Envision Rabbit kit for detection and secondary. I have only seen very faint staining and am wondering if anyone out there has worked with this and, if so, what type of control tissue would show the best signal. Thanks for any help, Michelle Mathews, HT(ASCP) TPL, UNC-CH From aazath <@t> hotmail.com Fri Jul 23 10:26:28 2010 From: aazath <@t> hotmail.com (Aazath Raj) Date: Fri Jul 23 10:26:33 2010 Subject: [Histonet] Artifacts in histology section In-Reply-To: References: Message-ID: Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aazath@hotmail.com _________________________________________________________________ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/ From leticia.figliuolo <@t> roche.com Fri Jul 23 10:34:59 2010 From: leticia.figliuolo <@t> roche.com (Figliuolo, Leticia) Date: Fri Jul 23 10:35:09 2010 Subject: [Histonet] Microwave Decalcification Message-ID: <069E6CF048B915488720412DAFD1474D01BA5D9AC7@RNUMSEM702.nala.roche.com> Hello all, We inherited a Pelco Biowave 34700 (from Ted Pella) and would like to optimize decalcification of rats knee joints, dogs rib using the Biowave. I was wondering if anyone have a protocol for decalcification using nitric acid or formic acid or EDTA using microwave or if you know someone that uses microwave technology for decalcification that I can ask. Your help is appreciated. Leticia From rjbuesa <@t> yahoo.com Fri Jul 23 10:59:06 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 23 10:59:10 2010 Subject: [Histonet] Artifacts in histology section In-Reply-To: Message-ID: <902127.95344.qm@web65704.mail.ac4.yahoo.com> Of the?3 probable causes, the most likely to be is the blade edge not uniformly?sharp Ren? J. --- On Fri, 7/23/10, Aazath Raj wrote: From: Aazath Raj Subject: [Histonet] Artifacts in histology section To: histonet@lists.utsouthwestern.edu Date: Friday, July 23, 2010, 11:26 AM Dear Friends, ? ? ? ? ? ? ???I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every???day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aazath@hotmail.com ??? ???????? ?????? ??? ? _________________________________________________________________ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Fri Jul 23 11:09:52 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Fri Jul 23 11:09:59 2010 Subject: [Histonet] artifacts of sectioning Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5488@MAIL1.pph.local> It sounds like what you are describing is what we call "chatter." it seems to occur most frequently with GI biopsies, esp colon polyps and usually in the abnormal epithelium. My techs tell me that cooling the block better on the ice block makes it go away. i have no personal experience, other than to say that when it occurs, it makes the slide of very poor quality. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- From c.weaver <@t> vla.defra.gsi.gov.uk Fri Jul 23 11:15:05 2010 From: c.weaver <@t> vla.defra.gsi.gov.uk (Weaver, Colin) Date: Fri Jul 23 11:15:20 2010 Subject: [Histonet] Formalin substitutes Message-ID: <0A1C03FC4E63EE4AA24A7983E91C25B502938034@vla-exchn1.cvlnt.vla.gov.uk> Hi everyone - does anyone know how effective these formalin substitutes are at killing microorgansms9esp HG3) in the tissue fixed? I know that Finefix states that because of the alcohol content being over 70% that it is effective against many micro-organisms but info seems scarce on the others Thanks and have a nice weekend Colin Weaver Histology lab manager VLA Thirsk North Yorkshire UK
Veterinary Laboratories Agency (VLA)

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From mbrooks <@t> incytepathology.com  Fri Jul 23 11:20:34 2010
From: mbrooks <@t> incytepathology.com (Matt Brooks)
Date: Fri Jul 23 11:20:39 2010
Subject: [Histonet] artifacts of sectioning
In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A5488@MAIL1.pph.local>
References: <2820431BF953BB4DA3E9E1A5882265FD034A5488@MAIL1.pph.local>
Message-ID: <706224670091FE47997AEF88EFADE7CA01550E89@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>

What I have used in the past is a short warm water soak, 10-15 seconds,
in the water bath, after facing in and between sections, then placing
the block back on ice. I would use a small 200 mL beaker filled with DI
water sitting inside the water, it helps reduce junk floating the bath.
Be careful to avoid over soaking. 

Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
mbrooks@incytepathology.com
509-892-2744


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Friday, July 23, 2010 9:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] artifacts of sectioning

It sounds like what you are describing is what we call "chatter."  it
seems to occur most frequently with GI biopsies, esp colon polyps and
usually in the abnormal epithelium.  My techs tell me that cooling the
block better on the ice block makes it go away.  i have no personal
experience, other than to say that when it occurs, it makes the slide of
very poor quality.
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

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From Rick.Garnhart <@t> memorialhealthsystem.com  Fri Jul 23 11:25:29 2010
From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com)
Date: Fri Jul 23 11:25:34 2010
Subject: [Histonet] HPV
In-Reply-To: 
Message-ID: 


We are doing the P16 antibody from MTM Labs.


Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph:  719-365-6926
Fax: 719-365-6373
rick.garnhart@memorialhealthsystem.com



Mission: To provide the highest quality health care
Vision: To create an outstanding health system where patients heal and people thrive
Values: Compassion - Integrity - Quality - Respect - Teamwork

www.memorialhealthsystem.com

The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s)
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From napoli <@t> mail.siscom.net  Fri Jul 23 13:04:22 2010
From: napoli <@t> mail.siscom.net (napoli@mail.siscom.net)
Date: Fri Jul 23 13:04:26 2010
Subject: [Histonet] Water collecting at bottom of sections
Message-ID: <4c49d9a6.99.6d62.528295481@siscom.net>

Hello all,

>From time to time and depending on what brand of adhesive
(or charged) slides I am using, I seem to get a "bag" of
water that drains to the bottom of my sections but doesn't
drain "out".

I have been working in microtomy a long time and have had to
deal with this contingency time and time again, but never
really have gotten to the bottom of the problem. I spoke
with a premium manufacturer of such slides and they seemed
to indicate that it is a problem with the coating, but
couldn't tell me for sure.

All I know is that certain brands do this more than others.
If you know what I mean, you know it is a problem. My bath
is pure distilled H2O with no gelatin or Sta-on added. It is
if the adhesive properties are SO good that they will not
release the water when vertically drained and have to be
shaken off or cut with a razor blade at bottom to release
the water.

Anyway, if anyone has an insight or two on this, I would be
interested. It seem sthe most challenging issues are ones
that seem related to some of the most simple tasks that one
has performed for many years!! Manufacturers understand what
I mean, but cannot pinpoint the problem for me via phone or
e-mail.

Anyone see this and have a chemical/mechanical solution they
have developed over the years?


Thanks!

From terri.j.waller <@t> gmail.com  Fri Jul 23 13:10:54 2010
From: terri.j.waller <@t> gmail.com (Terri Waller)
Date: Fri Jul 23 13:10:58 2010
Subject: [Histonet] thick slice IHC help!!
Message-ID: 

Hi all,
I'm a summer student and the project I was given was to optimize the
immunohistochemistry in my lab. BUT I'M NOT GETTING ANYTHING TO WORK!!
I've been told it always worked before....So I was wondering if anyone has
any ideas or protocols that work?   I'd really appreciate any help I can
get!
Here's the protocol I'm using:
our slices are 250-300 um thick.
1) I leave them in formalin for 2 days for the fixative step.
2) Wash slices 3X (15 min each) in PBS-T (PBS with 0.3% tritonX100 to help
permeabilize my slices since they're thick)
3) Blocking step for 1/2 hr with 2%normal horse serum in PBS-T
4) Incubate in 1o antibody at 1:1000 in PBS-T + 2%normal horse serum for 72
hrs at 4C with shaking
5) Wash slices 5X in PBS-T
6) Blocking step for 1/2 hr with 2%normal horse serum in PBS-T
7) Incubate at room temp with 2o antibody at 1:500 +2%normal horse serum in
PBS-T for 2hrs with shaking and kept in the dark
8 ) Wash 5X (1/2 hr each) in PBS before mounting
Terri
From LSebree <@t> uwhealth.org  Fri Jul 23 13:42:05 2010
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Fri Jul 23 13:42:09 2010
Subject: [Histonet] Water collecting at bottom of sections
In-Reply-To: <4c49d9a6.99.6d62.528295481@siscom.net>
References: <4c49d9a6.99.6d62.528295481@siscom.net>
Message-ID: <8C023B4AB999614BA4791BAEB26E2738399F20@UWHC-MAIL01.uwhis.hosp.wisc.edu>

I routinely "flick" my wrist holding the slide with the section I've
just picked up.  Usually, this is enough force to release any water at
the bottom of the section.   If that doesn't work  I melt a tiny hole at
the bottom of the section with a heated probe and flick again.  I'm sure
the manufacturer is right in saying that its the coating; the paraffin
adheres too well and too quickly to the slide trapping water underneath.
It is also very important to hold your slides as vertically as possible
when bringing them under your section and raising the slide out of the
water bath.  That way you will trap as little water as possible
underneath.

Hope this helps.

>From one long-time-microtomist to another,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
napoli@mail.siscom.net
Sent: Friday, July 23, 2010 1:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water collecting at bottom of sections

Hello all,

>From time to time and depending on what brand of adhesive
(or charged) slides I am using, I seem to get a "bag" of water that
drains to the bottom of my sections but doesn't drain "out".

I have been working in microtomy a long time and have had to deal with
this contingency time and time again, but never really have gotten to
the bottom of the problem. I spoke with a premium manufacturer of such
slides and they seemed to indicate that it is a problem with the
coating, but couldn't tell me for sure.

All I know is that certain brands do this more than others.
If you know what I mean, you know it is a problem. My bath is pure
distilled H2O with no gelatin or Sta-on added. It is if the adhesive
properties are SO good that they will not release the water when
vertically drained and have to be shaken off or cut with a razor blade
at bottom to release the water.

Anyway, if anyone has an insight or two on this, I would be interested.
It seem sthe most challenging issues are ones that seem related to some
of the most simple tasks that one has performed for many years!!
Manufacturers understand what I mean, but cannot pinpoint the problem
for me via phone or e-mail.

Anyone see this and have a chemical/mechanical solution they have
developed over the years?


Thanks!

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From histonet.nospam <@t> vneubert.com  Fri Jul 23 14:01:07 2010
From: histonet.nospam <@t> vneubert.com (V. Neubert)
Date: Fri Jul 23 14:01:15 2010
Subject: [Histonet] Water collecting at bottom of sections
In-Reply-To: <4c49d9a6.99.6d62.528295481@siscom.net>
References: <4c49d9a6.99.6d62.528295481@siscom.net>
Message-ID: <4C49E6F3.2020202@vneubert.com>

I experienced it almost daily and thought this was normal!?

I just dipped the slides gently on a paper towel right after getting 
them on the slide and shaking, for the case I could not just shake it 
off. Indeed, as I remember, the Super Frost Plus slides had to be dipped 
a bit harder to let the water rinse off.


> Hello all,
>
> > From time to time and depending on what brand of adhesive
> (or charged) slides I am using, I seem to get a "bag" of
> water that drains to the bottom of my sections but doesn't
> drain "out".
>
> I have been working in microtomy a long time and have had to
> deal with this contingency time and time again, but never
> really have gotten to the bottom of the problem. I spoke
> with a premium manufacturer of such slides and they seemed
> to indicate that it is a problem with the coating, but
> couldn't tell me for sure.
>
> All I know is that certain brands do this more than others.
> If you know what I mean, you know it is a problem. My bath
> is pure distilled H2O with no gelatin or Sta-on added. It is
> if the adhesive properties are SO good that they will not
> release the water when vertically drained and have to be
> shaken off or cut with a razor blade at bottom to release
> the water.
>
> Anyway, if anyone has an insight or two on this, I would be
> interested. It seem sthe most challenging issues are ones
> that seem related to some of the most simple tasks that one
> has performed for many years!! Manufacturers understand what
> I mean, but cannot pinpoint the problem for me via phone or
> e-mail.
>
> Anyone see this and have a chemical/mechanical solution they
> have developed over the years?
>
>
> Thanks!
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>    


From cpyse <@t> x-celllab.com  Fri Jul 23 14:11:16 2010
From: cpyse <@t> x-celllab.com (Cynthia Pyse)
Date: Fri Jul 23 14:11:13 2010
Subject: [Histonet] Water collecting at bottom of sections
In-Reply-To: <8C023B4AB999614BA4791BAEB26E2738399F20@UWHC-MAIL01.uwhis.hosp.wisc.edu>
References: <4c49d9a6.99.6d62.528295481@siscom.net>
	<8C023B4AB999614BA4791BAEB26E2738399F20@UWHC-MAIL01.uwhis.hosp.wisc.edu>
Message-ID: <001f01cb2a9a$d30bfee0$7923fca0$@com>

I second Linda's technique, the only other option I use is to bring the
slide up at a negative angle so the slide comes out of the water bath first
then the section. I find this works well for us.

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse@x-celllab.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda
A
Sent: Friday, July 23, 2010 2:42 PM
To: napoli@mail.siscom.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Water collecting at bottom of sections

I routinely "flick" my wrist holding the slide with the section I've
just picked up.  Usually, this is enough force to release any water at
the bottom of the section.   If that doesn't work  I melt a tiny hole at
the bottom of the section with a heated probe and flick again.  I'm sure
the manufacturer is right in saying that its the coating; the paraffin
adheres too well and too quickly to the slide trapping water underneath.
It is also very important to hold your slides as vertically as possible
when bringing them under your section and raising the slide out of the
water bath.  That way you will trap as little water as possible
underneath.

Hope this helps.

>From one long-time-microtomist to another,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
napoli@mail.siscom.net
Sent: Friday, July 23, 2010 1:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water collecting at bottom of sections

Hello all,

>From time to time and depending on what brand of adhesive
(or charged) slides I am using, I seem to get a "bag" of water that
drains to the bottom of my sections but doesn't drain "out".

I have been working in microtomy a long time and have had to deal with
this contingency time and time again, but never really have gotten to
the bottom of the problem. I spoke with a premium manufacturer of such
slides and they seemed to indicate that it is a problem with the
coating, but couldn't tell me for sure.

All I know is that certain brands do this more than others.
If you know what I mean, you know it is a problem. My bath is pure
distilled H2O with no gelatin or Sta-on added. It is if the adhesive
properties are SO good that they will not release the water when
vertically drained and have to be shaken off or cut with a razor blade
at bottom to release the water.

Anyway, if anyone has an insight or two on this, I would be interested.
It seem sthe most challenging issues are ones that seem related to some
of the most simple tasks that one has performed for many years!!
Manufacturers understand what I mean, but cannot pinpoint the problem
for me via phone or e-mail.

Anyone see this and have a chemical/mechanical solution they have
developed over the years?


Thanks!

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From collette2 <@t> llnl.gov  Fri Jul 23 15:06:20 2010
From: collette2 <@t> llnl.gov (Collette, Nicole M.)
Date: Fri Jul 23 15:06:25 2010
Subject: [Histonet] Water collecting at bottom of sections
In-Reply-To: <4c49d9a6.99.6d62.528295481@siscom.net>
References: <4c49d9a6.99.6d62.528295481@siscom.net>
Message-ID: <3AD1C8AEFB40D7408467F3A333A8A6D1019A37F80394@NSPEXMBX-B.the-lab.llnl.gov>

I use FisherBrand SuperFrost Plus charged slides, the only time I have this problem is when my waterbath is too hot, or if for some reason I use warmed slides to retrieve my sections from the bath (like if you lay the unused slides on the edge of the waterbath, or if you use the warm droplet method to spread your sections)? I haven't had much experience with a lot of different slide types/brands though, but it might be an easy fix to the problem you hadn't thought about, as it is more or less unrelated to the brand of slide, although some brands may be more sensitive to this issue...

Sincerely,
Nicole Collette
Lawrence Livermore National Lab/ UC Berkeley 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of napoli@mail.siscom.net
Sent: Friday, July 23, 2010 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water collecting at bottom of sections

Hello all,

>From time to time and depending on what brand of adhesive
(or charged) slides I am using, I seem to get a "bag" of
water that drains to the bottom of my sections but doesn't
drain "out".

I have been working in microtomy a long time and have had to
deal with this contingency time and time again, but never
really have gotten to the bottom of the problem. I spoke
with a premium manufacturer of such slides and they seemed
to indicate that it is a problem with the coating, but
couldn't tell me for sure.

All I know is that certain brands do this more than others.
If you know what I mean, you know it is a problem. My bath
is pure distilled H2O with no gelatin or Sta-on added. It is
if the adhesive properties are SO good that they will not
release the water when vertically drained and have to be
shaken off or cut with a razor blade at bottom to release
the water.

Anyway, if anyone has an insight or two on this, I would be
interested. It seem sthe most challenging issues are ones
that seem related to some of the most simple tasks that one
has performed for many years!! Manufacturers understand what
I mean, but cannot pinpoint the problem for me via phone or
e-mail.

Anyone see this and have a chemical/mechanical solution they
have developed over the years?


Thanks!

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://*lists.utsouthwestern.edu/mailman/listinfo/histonet


From talulahgosh <@t> gmail.com  Fri Jul 23 16:35:34 2010
From: talulahgosh <@t> gmail.com (Emily Sours)
Date: Fri Jul 23 16:35:38 2010
Subject: [Histonet] Water collecting at bottom of sections
In-Reply-To: <3AD1C8AEFB40D7408467F3A333A8A6D1019A37F80394@NSPEXMBX-B.the-lab.llnl.gov>
References: <4c49d9a6.99.6d62.528295481@siscom.net>
	<3AD1C8AEFB40D7408467F3A333A8A6D1019A37F80394@NSPEXMBX-B.the-lab.llnl.gov>
Message-ID: 

Am I the only person who paraffin sections by: cutting the sections,
grabbing the ribbon with forceps or paintbrush, placing it on a dry slide,
adding water underneath the ribbon with a pastuer pipet, and then placing
the slide on a slide warmer.  The water will heat up and the paraffin ribbon
will expand on the bubble of water on the slide.Leave the slide on a slide
warmer overnight and the water is evaporates.  Some people blot off the
extra water.
Maybe that's just not any easier than picking up the sections from a water
bath.

Emily
--
Outside of a dog, a book is man's best friend. Inside of a dog it's too dark
to read.
--Groucho Marx
From faith14913 <@t> aol.com  Fri Jul 23 19:21:40 2010
From: faith14913 <@t> aol.com (faith14913@aol.com)
Date: Fri Jul 23 19:21:50 2010
Subject: [Histonet] artifacts in sections
In-Reply-To: 
References: 
Message-ID: <2043131906-1279930900-cardhu_decombobulator_blackberry.rim.net-1433929939-@bda2190.bisx.prod.on.blackberry>

I have always soaked my biopsies on ice water.  It always seems to help.

Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: histonet-request@lists.utsouthwestern.edu
Sender: histonet-bounces@lists.utsouthwestern.edu
Date: Fri, 23 Jul 2010 13:01:46 
To: 
Reply-To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 80, Issue 27

Send Histonet mailing list submissions to
    histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Laboratory Labeling Systems... (Barone, Carol )
   2. Re: Histonet Digest, Vol 80, Issue 26 (Stephanie Rodriguez)
   3. Histology metrics (Scherer, Kris)
   4. RE: HPV testing (McMahon, Loralee A)
   5. RE: Biocare P63 with Dako Flex detection (Cynthia Pyse)
   6. biohazard bags (Jeff Birkner)
   7. RE: biohazard bags (Stacy McLaughlin)
   8. Re: biohazard bags (DKBoyd@chs.net)
   9. Sectioning problem (kgrobert@rci.rutgers.edu)
  10. Re: Sectioning problem (Bryan Llewellyn)
  11. Staining dish for vertical 30-slide rack (Morken, Tim)
  12. RE: biohazard bags (Rathborne, Toni)
  13. p-TBK-1 Ab (Michelle Mathews)
  14. Artifacts in histology section (Aazath Raj)
  15. Microwave Decalcification (Figliuolo, Leticia)
  16. Re: Artifacts in histology section (Rene J Buesa)
  17. artifacts of sectioning (Tench, Bill)
  18. Formalin substitutes (Weaver, Colin)
  19. RE: artifacts of sectioning (Matt Brooks)
  20. HPV (Rick.Garnhart@memorialhealthsystem.com)


----------------------------------------------------------------------

Message: 1
Date: Thu, 22 Jul 2010 13:21:26 -0400
From: "Barone, Carol " 
Subject: [Histonet] Laboratory Labeling Systems...
To: 
Message-ID:
    <37E4BAC017F57141AF64FAA5AEB04CE8033A7E87@wlmmsx01.nemours.org>
Content-Type: text/plain;   charset="us-ascii"

Histonetters....What are your thoughts: 
 
We are getting ready to purchase a labeling system for our Core
Facility. We have limited $'s and have demo'ed three systems. We have
many different projects,with varying numbers of samples, blocks and
slides. We would like to cover the widest amount of needs, with the
system....not just cassettes and slides, but samples in freezers to
antibodies we have alloquotted. We are about to purchase a two-D
bar-coding system that can label everything we need and work with our
present EXCEL data bases.  We are leaning toward a Brady System, where
we can purchase individually the parts we need for labeling cassettes to
eppy's and we especially like that they... not only have a label that
can be attached to frozen vials... but that there are several types of
scanners available for the different stations in our lab. This system
meets our CER requirements and is up-gradable for adding more stations
if needed at alater time. 
 
Sounds too good to be true....so that is where you all come in. 
 
Is anyone using a Brady Labeling System? Good? Bad? Problems? What are
your thoughts?  Thanks for the impromptu survey. We plan to order in
August, if there are no "big" issues, with those that use it. We demo's
the labels and are happy with them. We are going alpha numeric/2-D...3
lines of text, is needed for our research work. Thanks for your
thoughts. 
 
C. Barone
Director Histotechnology Core Lab
cbarone@nemours.org
 


------------------------------

Message: 2
Date: Thu, 22 Jul 2010 10:44:10 -0700
From: Stephanie Rodriguez 
Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 26
To: 
Message-ID: 
Content-Type: text/plain;   charset="US-ASCII"

As do we.


Stephanie Rodriguez, HTL(ASCP), QIHC
Senior Molecular Technologist-FISH
IHC Technologist III
Phenopath Laboratories
Seattle, WA
(206) 374-9000 




On 7/22/10 10:04 AM, "histonet-request@lists.utsouthwestern.edu"
 wrote:

> 
> Message: 4
> Date: Thu, 22 Jul 2010 08:18:27 -0700
> From: "Tench, Bill" 
> Subject: [Histonet] hpv testing on head and neck ca's
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5480@MAIL1.pph.local>
> Content-Type: text/plain; charset=us-ascii
> 
> We do p16 on squamous ca of the head and neck region routinely
>  
> Bill Tench
> Associate Dir. Laboratory Services
> Chief, Cytology Services
> Palomar Medical Center
> 555 E. Valley Parkway
> Escondido, California  92025
> Bill.Tench@pph.org
> Voice: 760- 739-3037
> Fax: 760-739-2604



This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.


------------------------------

Message: 3
Date: Thu, 22 Jul 2010 13:43:52 -0400
From: "Scherer, Kris" 
Subject: [Histonet] Histology metrics
To: "histonet@lists.utsouthwestern.edu"
    
Message-ID:
    <0C15E5FC826B334BA019E489846D02357E06C23C55@RTWECS03.lca.labcorp.com>
Content-Type: text/plain; charset="us-ascii"

We are in need of a metrics for use in requesting an additional histotech.  Does anyone have a system in place for presenting the need for an additional FTE, based on volume, worklow, vacations, etc?
----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. 




------------------------------

Message: 4
Date: Thu, 22 Jul 2010 13:47:54 -0400
From: "McMahon, Loralee A" 
Subject: RE: [Histonet] HPV testing
To: Richard Cartun , Histonet
    
Message-ID:
    
    
Content-Type: text/plain; charset="us-ascii"

Hi Dr. Cartun, 

We are running the HPV Genepoint test from Dako (in situ test).  Along with the EGFR IHC.  


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org]
Sent: Thursday, July 22, 2010 11:01 AM
To: Histonet
Subject: [Histonet] HPV testing

What are labs doing for HPV testing on head and neck cancers when a request is received from a clinician?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 5
Date: Thu, 22 Jul 2010 15:10:24 -0400
From: "Cynthia Pyse" 
Subject: RE: [Histonet] Biocare P63 with Dako Flex detection
To: ,   
Message-ID: <000001cb29d1$89f157b0$9dd40710$@com>
Content-Type: text/plain;   charset="us-ascii"

Cathy
Have you tried adding an EnVision FLEX mouse linker to your protocol.
Sometimes that will work.

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse@x-celllab.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Cathy.Crumpton@tuality.org
Sent: Wednesday, July 21, 2010 3:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Biocare P63 with Dako Flex detection


   Hi  all, I have ran into a troubleshooting dilemma and was wonderi=g
   if  anyone  else could help.  Is there anyone out there that uses the
Biocare  Medical  P63 concentrated antibody with the Dako TRS and Flex
   detec=tion  kit?  I am curious what dilution you use and if you have
   to  do  an=ything  special  for  the  P63 (extra long retrieval, DAB
   enhancer, etc). = am having problems getting a strong signal for the
   basal cells in prosta=e.  I am running it at 1:50 with 40 minutes in
   pH  9  retrieval  (which  =seems  too  long to me but does help).  A
   dilution with the same protoco= at 1:100 is way too light.

   Thanks,
    <=DIV>
   Cathy Crumpton HT(ASCP), Histology Lead
   Tuality Community Hosp=tal
   Hillsboro, OR 97123
   (503)681-1292
_______________________________________________
Histonet mailing list
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------------------------------

Message: 6
Date: Thu, 22 Jul 2010 14:11:18 -0500
From: "Jeff Birkner" 
Subject: [Histonet] biohazard bags
To: 
Message-ID:
    
Content-Type: text/plain;   charset="us-ascii"

We are looking into our current use of biohazard bags.  How many of you
are currently re-using any bags for specimen transport?  These would
only be bags were the actual specimen was inside a secondary container
and thus never directly came into contact with the bags.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirkner@colabserv.com

 


The information contained in this communication may be confidential, is intended
only for the use of the recipient(s) named above, and may be legally privileged.
If the reader of this message is not the intended recipient, you are hereby 
notified that any dissemination, distribution, or copying of this communication,
or any of its contents, is strictly prohibited.  If you have received this
communication in error, please return it to the sender immediately and delete the
original message and any copy of it from your computer system.  If you have any
questions concerning this message, please contact the sender. 


------------------------------

Message: 7
Date: Thu, 22 Jul 2010 15:38:01 -0400
From: "Stacy McLaughlin" 
Subject: RE: [Histonet] biohazard bags
To: "Jeff Birkner" ,
    
Message-ID:
    
    
Content-Type: text/plain;   charset="iso-8859-1"

Re-use of specimen transport bio-bags is a big NO-NO in my laboratory.  You run the risk of accidentally using one that is contaminated. (This actually happened, and it was not a pretty sight.) 
You can't guarantee that the outside of that specimen container in the bag is not contaminated, even though it looks clean.  You don't know the practices of the person that collected it.  This could be a big liability and the possible consequences just aren't worth it.  
Just my 2 cents.
Stacy 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jeff
Birkner
Sent: Thursday, July 22, 2010 3:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] biohazard bags


We are looking into our current use of biohazard bags.  How many of you
are currently re-using any bags for specimen transport?  These would
only be bags were the actual specimen was inside a secondary container
and thus never directly came into contact with the bags.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirkner@colabserv.com

 


The information contained in this communication may be confidential, is intended
only for the use of the recipient(s) named above, and may be legally privileged.
If the reader of this message is not the intended recipient, you are hereby 
notified that any dissemination, distribution, or copying of this communication,
or any of its contents, is strictly prohibited.  If you have received this
communication in error, please return it to the sender immediately and delete the
original message and any copy of it from your computer system.  If you have any
questions concerning this message, please contact the sender. 
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 8
Date: Thu, 22 Jul 2010 15:42:34 -0400
From: DKBoyd@chs.net
Subject: Re: [Histonet] biohazard bags
To: "Jeff Birkner" 
Cc: histonet@lists.utsouthwestern.edu,
    histonet-bounces@lists.utsouthwestern.edu
Message-ID:
    
Content-Type: text/plain;   charset="US-ASCII"

We were doing just that and our Infection Control Nurse has banded this 
practice.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd@chs.net







"Jeff Birkner"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
07/22/2010 03:11 PM

To

cc

Subject
[Histonet] biohazard bags






We are looking into our current use of biohazard bags.  How many of you
are currently re-using any bags for specimen transport?  These would
only be bags were the actual specimen was inside a secondary container
and thus never directly came into contact with the bags.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirkner@colabserv.com

 


The information contained in this communication may be confidential, is 
intended
only for the use of the recipient(s) named above, and may be legally 
privileged.
If the reader of this message is not the intended recipient, you are 
hereby 
notified that any dissemination, distribution, or copying of this 
communication,
or any of its contents, is strictly prohibited.  If you have received this
communication in error, please return it to the sender immediately and 
delete the
original message and any copy of it from your computer system.  If you 
have any
questions concerning this message, please contact the sender. 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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--------------------------------------------------------------------------
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------------------------------

Message: 9
Date: Thu, 22 Jul 2010 16:12:56 -0400 (EDT)
From: kgrobert@rci.rutgers.edu
Subject: [Histonet] Sectioning problem
To: "histonet" 
Message-ID:
    <8b576f6ad1bfefb4659958e4c7c10cee.squirrel@webmail.rci.rutgers.edu>
Content-Type: text/plain;charset=iso-8859-1

I am working on an old microtome, a Reichert Histostat with a Tissue-Tek
II microtome chuck adapter, and the last time I had this machine cleaned
and rehabbed by my repairman, he said that the springs were shot on the
chuck adapter, resulting in the windowblind chatter that I was getting in
my sections. Unfortunately he did not have a replacement, Sakura doesn't
make or sell them anymore, and the one source for replacement adapters I
did find online sold me a part that had the same problem-shot springs (I
assume this, as I was getting the same chatter with the "new" part).  I
have Googled, searched on eBay, checked online used scientific/medical
equipment suppliers to no avail...but I will keep looking in case
something comes up.

So I guess my question is...would one of the newer cassette holders fit in
my microtomes?  In addition to the Reichert mentioned above, I also have
an American Optical 860.  Or is it possible to figure out how to replace
the springs in the thing? (I have half a mind to wander over to the
mechanical engineering dept. here to ask that question, but I figured I'd
start here on Histonet.) I would really hate to have to replace the
microtomes just because I can't get a cassette holder for them.

Thanks for all your help,

Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(732) 445-6914



------------------------------

Message: 10
Date: Thu, 22 Jul 2010 14:02:24 -0700
From: "Bryan Llewellyn" 
Subject: Re: [Histonet] Sectioning problem
To: "Histonet" 
Message-ID: <9CAE5B0417C54C3D9F56AB57B40271D0@BryanPC>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
    reply-type=original

I had spring problems on the block holder of a microtome many years ago.  I 
solved it by getting a local engineering shop to make me some new springs 
using the old springs as a template.  It worked fine, and was cheap, too.

Bryan Llewellyn


----- Original Message ----- 
From: 
To: "histonet" 
Sent: Thursday, July 22, 2010 1:12 PM
Subject: [Histonet] Sectioning problem


I am working on an old microtome, a Reichert Histostat with a Tissue-Tek
II microtome chuck adapter, and the last time I had this machine cleaned
and rehabbed by my repairman, he said that the springs were shot on the
chuck adapter, resulting in the windowblind chatter that I was getting in
my sections. Unfortunately he did not have a replacement, Sakura doesn't
make or sell them anymore, and the one source for replacement adapters I
did find online sold me a part that had the same problem-shot springs (I
assume this, as I was getting the same chatter with the "new" part).  I
have Googled, searched on eBay, checked online used scientific/medical
equipment suppliers to no avail...but I will keep looking in case
something comes up.

So I guess my question is...would one of the newer cassette holders fit in
my microtomes?  In addition to the Reichert mentioned above, I also have
an American Optical 860.  Or is it possible to figure out how to replace
the springs in the thing? (I have half a mind to wander over to the
mechanical engineering dept. here to ask that question, but I figured I'd
start here on Histonet.) I would really hate to have to replace the
microtomes just because I can't get a cassette holder for them.

Thanks for all your help,

Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(732) 445-6914

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 11
Date: Thu, 22 Jul 2010 15:40:45 -0700
From: "Morken, Tim" 
Subject: [Histonet] Staining dish for vertical 30-slide rack
To: "histonet@lists.utsouthwestern.edu"
    
Message-ID:
    <1AAF670737F193429070841C6B2ADD4C023D27BD4D@EXMBMCB15.ucsfmedicalcenter.org>
    
Content-Type: text/plain; charset=us-ascii

Does anyone know of a plastic, inexpensive staining dish for the Richard-Allan or Raymond Lamb 30-slide vertical rack. Something similar to the tissue tek plastic stain dish. The Rack is 11cm / 4 3/8" long


Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
Box 1656
1600 Divisadero St.
San Francisco, CA 94143-1656
USA

Phone: (415) 514-6042
Pager: (415) 443-6509
Fax: (415) 885-7409

Email: tim.morken@ucsfmedctr.org



------------------------------

Message: 12
Date: Fri, 23 Jul 2010 08:04:48 -0400
From: "Rathborne, Toni" 
Subject: RE: [Histonet] biohazard bags
To: "Stacy McLaughlin" , "Jeff
    Birkner" ,  
Message-ID:
    
    
Content-Type: text/plain;  charset="utf-8"

I agree. We never re-use them at my facility.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Stacy
McLaughlin
Sent: Thursday, July 22, 2010 3:38 PM
To: Jeff Birkner; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] biohazard bags


Re-use of specimen transport bio-bags is a big NO-NO in my laboratory.  You run the risk of accidentally using one that is contaminated. (This actually happened, and it was not a pretty sight.) 
You can't guarantee that the outside of that specimen container in the bag is not contaminated, even though it looks clean.  You don't know the practices of the person that collected it.  This could be a big liability and the possible consequences just aren't worth it.  
Just my 2 cents.
Stacy 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jeff
Birkner
Sent: Thursday, July 22, 2010 3:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] biohazard bags


We are looking into our current use of biohazard bags.  How many of you
are currently re-using any bags for specimen transport?  These would
only be bags were the actual specimen was inside a secondary container
and thus never directly came into contact with the bags.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirkner@colabserv.com

 


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------------------------------

Message: 13
Date: Fri, 23 Jul 2010 07:15:58 -0700 (PDT)
From: Michelle Mathews 
Subject: [Histonet] p-TBK-1 Ab
To: histonet@lists.utsouthwestern.edu
Message-ID: <510051.54589.qm@web53308.mail.re2.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Good morning everyone,
I work in a research lab and am trying to optimize a p-TBK-1 antibody; I am 
running this work-up on a BondIII and a BondMax with a Dako Envision Rabbit kit 
for detection and secondary. I have only seen very faint staining and am 
wondering if anyone out there has worked with this and, if so, what type of 
control tissue would show the best signal.
Thanks for any help,
Michelle Mathews, HT(ASCP)
TPL, UNC-CH



      

------------------------------

Message: 14
Date: Fri, 23 Jul 2010 20:56:28 +0530
From: Aazath Raj 
Subject: [Histonet] Artifacts in histology section
To: 
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"



 Dear Friends,

               I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every   day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section.

 

 

with regards,

Aazathraj.P

Technical Officer,

Apollo Hospitals-chennai

India.

aazath@hotmail.com

 
                      
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------------------------------

Message: 15
Date: Fri, 23 Jul 2010 11:34:59 -0400
From: "Figliuolo, Leticia" 
Subject: [Histonet] Microwave Decalcification
To: "'histonet@lists.utsouthwestern.edu'"
    
Message-ID:
    <069E6CF048B915488720412DAFD1474D01BA5D9AC7@RNUMSEM702.nala.roche.com>
Content-Type: text/plain; charset="us-ascii"

Hello all,

We inherited a Pelco Biowave 34700 (from Ted Pella) and would like to optimize decalcification of rats knee joints, dogs rib using the Biowave. I was wondering if anyone have a protocol for decalcification using nitric acid or formic acid or EDTA using microwave or if you know someone that uses microwave technology for decalcification that I can ask.

Your help is appreciated.
Leticia


------------------------------

Message: 16
Date: Fri, 23 Jul 2010 08:59:06 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Artifacts in histology section
To: histonet@lists.utsouthwestern.edu, Aazath Raj 
Message-ID: <902127.95344.qm@web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Of the?3 probable causes, the most likely to be is the blade edge not uniformly?sharp
Ren? J.
--- On Fri, 7/23/10, Aazath Raj  wrote:


From: Aazath Raj 
Subject: [Histonet] Artifacts in histology section
To: histonet@lists.utsouthwestern.edu
Date: Friday, July 23, 2010, 11:26 AM




Dear Friends,

? ? ? ? ? ? ???I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every???day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section.





with regards,

Aazathraj.P

Technical Officer,

Apollo Hospitals-chennai

India.

aazath@hotmail.com


??? ???????? ?????? ??? ? 
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------------------------------

Message: 17
Date: Fri, 23 Jul 2010 09:09:52 -0700
From: "Tench, Bill" 
Subject: [Histonet] artifacts of sectioning
To: histonet@lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5488@MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii

It sounds like what you are describing is what we call "chatter."  it
seems to occur most frequently with GI biopsies, esp colon polyps and
usually in the abnormal epithelium.  My techs tell me that cooling the
block better on the ice block makes it go away.  i have no personal
experience, other than to say that when it occurs, it makes the slide of
very poor quality.
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

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------------------------------

Message: 18
Date: Fri, 23 Jul 2010 17:15:05 +0100
From: "Weaver, Colin" 
Subject: [Histonet] Formalin substitutes
To: 
Message-ID:
    <0A1C03FC4E63EE4AA24A7983E91C25B502938034@vla-exchn1.cvlnt.vla.gov.uk>
Content-Type: text/plain; charset="us-ascii"

Hi everyone - does anyone know how effective these formalin substitutes
are at killing microorgansms9esp HG3) in the tissue fixed? I know that
Finefix states that because of the alcohol content being over 70% that
it is effective against many micro-organisms but info seems scarce on
the others

 

Thanks and have a nice weekend

 

Colin Weaver

Histology lab manager

VLA

Thirsk

North Yorkshire

UK

Veterinary Laboratories Agency (VLA)

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------------------------------

Message: 19
Date: Fri, 23 Jul 2010 09:20:34 -0700
From: "Matt Brooks" 
Subject: RE: [Histonet] artifacts of sectioning
To: 
Message-ID:
    <706224670091FE47997AEF88EFADE7CA01550E89@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>
    
Content-Type: text/plain;   charset="us-ascii"

What I have used in the past is a short warm water soak, 10-15 seconds,
in the water bath, after facing in and between sections, then placing
the block back on ice. I would use a small 200 mL beaker filled with DI
water sitting inside the water, it helps reduce junk floating the bath.
Be careful to avoid over soaking. 

Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
mbrooks@incytepathology.com
509-892-2744


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Friday, July 23, 2010 9:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] artifacts of sectioning

It sounds like what you are describing is what we call "chatter."  it
seems to occur most frequently with GI biopsies, esp colon polyps and
usually in the abnormal epithelium.  My techs tell me that cooling the
block better on the ice block makes it go away.  i have no personal
experience, other than to say that when it occurs, it makes the slide of
very poor quality.
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

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------------------------------

Message: 20
Date: Fri, 23 Jul 2010 10:25:29 -0600
From: Rick.Garnhart@memorialhealthsystem.com
Subject: [Histonet] HPV
To: histonet@lists.utsouthwestern.edu
Message-ID:
    
    
Content-Type: text/plain; charset=US-ASCII


We are doing the P16 antibody from MTM Labs.


Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph:  719-365-6926
Fax: 719-365-6373
rick.garnhart@memorialhealthsystem.com



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------------------------------

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End of Histonet Digest, Vol 80, Issue 27
****************************************
From amosbrooks <@t> gmail.com  Fri Jul 23 20:40:52 2010
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Fri Jul 23 20:40:56 2010
Subject: [Histonet] Buffers
Message-ID: 

Always,  Always, Always pH your buffers when you make them and adjust them
properly. If you do not you will NEVER know if your buffer is really at the
pH you think it is. You can't pour out 10 liters of water exactly the same
every time. You can't be sure the factory weighed the salts perfectly every
time. It is just a bad idea to assume these things are right and blame
variations on the pH of the water expecting unbuffered water to be a
constant.

Ok, getting off the soapbox,
Amos
From pruegg <@t> ihctech.net  Sat Jul 24 11:15:10 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sat Jul 24 11:15:54 2010
Subject: SPAM-LOW:  [Histonet] Laboratory Labeling Systems...
In-Reply-To: <37E4BAC017F57141AF64FAA5AEB04CE8033A7E87@wlmmsx01.nemours.org>
References: <37E4BAC017F57141AF64FAA5AEB04CE8033A7E87@wlmmsx01.nemours.org>
Message-ID: 

Carol,

The Brady labeling system is good but we opted for something that prints
directly on cassettes, slides, etc. to get around the peel and stick label
system, which not only is more work, it also introduces another concern for
mislabeling.  They are a lot more expensive but in the busy lab and with our
constant concerns for labeling errors this is the way we went, we got the
Leica labeling system which interfaces with the specimen logging system so
that a sample is logged once and from that all labels for cassettes, blocks,
reports, everything are generated.

Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone,
Carol 
Sent: Thursday, July 22, 2010 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Laboratory Labeling Systems...
Importance: High

Histonetters....What are your thoughts: 
 
We are getting ready to purchase a labeling system for our Core
Facility. We have limited $'s and have demo'ed three systems. We have
many different projects,with varying numbers of samples, blocks and
slides. We would like to cover the widest amount of needs, with the
system....not just cassettes and slides, but samples in freezers to
antibodies we have alloquotted. We are about to purchase a two-D
bar-coding system that can label everything we need and work with our
present EXCEL data bases.  We are leaning toward a Brady System, where
we can purchase individually the parts we need for labeling cassettes to
eppy's and we especially like that they... not only have a label that
can be attached to frozen vials... but that there are several types of
scanners available for the different stations in our lab. This system
meets our CER requirements and is up-gradable for adding more stations
if needed at alater time. 
 
Sounds too good to be true....so that is where you all come in. 
 
Is anyone using a Brady Labeling System? Good? Bad? Problems? What are
your thoughts?  Thanks for the impromptu survey. We plan to order in
August, if there are no "big" issues, with those that use it. We demo's
the labels and are happy with them. We are going alpha numeric/2-D...3
lines of text, is needed for our research work. Thanks for your
thoughts. 
 
C. Barone
Director Histotechnology Core Lab
cbarone@nemours.org
 
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From pruegg <@t> ihctech.net  Sat Jul 24 11:26:17 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sat Jul 24 11:27:00 2010
Subject: SPAM-LOW:  RE: [Histonet] Water collecting at bottom of sections
In-Reply-To: <3AD1C8AEFB40D7408467F3A333A8A6D1019A37F80394@NSPEXMBX-B.the-lab.llnl.gov>
References: <4c49d9a6.99.6d62.528295481@siscom.net>
	<3AD1C8AEFB40D7408467F3A333A8A6D1019A37F80394@NSPEXMBX-B.the-lab.llnl.gov>
Message-ID: <97D805A2BD274ACF9A94B7CF6A48974B@prueggihctechlt>

I always tap the slide on the counter before standing it up to drain after
picking it up off the water bath, I think this should shake loose the bag of
water you are talking about collecting under some sections.  I actually hit
the non label end of the slide on the counter pretty hard, I have had people
ask me if I ever brake the slide, but I have not ever broken a slide by
doing this.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Collette,
Nicole M.
Sent: Friday, July 23, 2010 2:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: RE: [Histonet] Water collecting at bottom of sections

I use FisherBrand SuperFrost Plus charged slides, the only time I have this
problem is when my waterbath is too hot, or if for some reason I use warmed
slides to retrieve my sections from the bath (like if you lay the unused
slides on the edge of the waterbath, or if you use the warm droplet method
to spread your sections)? I haven't had much experience with a lot of
different slide types/brands though, but it might be an easy fix to the
problem you hadn't thought about, as it is more or less unrelated to the
brand of slide, although some brands may be more sensitive to this issue...

Sincerely,
Nicole Collette
Lawrence Livermore National Lab/ UC Berkeley 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
napoli@mail.siscom.net
Sent: Friday, July 23, 2010 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water collecting at bottom of sections

Hello all,

>From time to time and depending on what brand of adhesive
(or charged) slides I am using, I seem to get a "bag" of
water that drains to the bottom of my sections but doesn't
drain "out".

I have been working in microtomy a long time and have had to
deal with this contingency time and time again, but never
really have gotten to the bottom of the problem. I spoke
with a premium manufacturer of such slides and they seemed
to indicate that it is a problem with the coating, but
couldn't tell me for sure.

All I know is that certain brands do this more than others.
If you know what I mean, you know it is a problem. My bath
is pure distilled H2O with no gelatin or Sta-on added. It is
if the adhesive properties are SO good that they will not
release the water when vertically drained and have to be
shaken off or cut with a razor blade at bottom to release
the water.

Anyway, if anyone has an insight or two on this, I would be
interested. It seem sthe most challenging issues are ones
that seem related to some of the most simple tasks that one
has performed for many years!! Manufacturers understand what
I mean, but cannot pinpoint the problem for me via phone or
e-mail.

Anyone see this and have a chemical/mechanical solution they
have developed over the years?


Thanks!

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From mike <@t> pathview.com  Sat Jul 24 22:52:09 2010
From: mike <@t> pathview.com (Michael Mihalik)
Date: Sun Jul 25 08:27:47 2010
Subject: [Histonet] Vendors of Specimen Tracking Systems
In-Reply-To: 
References: 
Message-ID: <032801cb2bfd$21cc0490$65640db0$@com>

>From a vendor's perspective, we have not heard anything bad about any of
these vendors.  However, something else you might want to keep in mind is
the objective of your implementation and what constraints you have on your
implementation.

For instance, at the most basic level, I've seen implementations where the
sole objective was to address the issue of illegible writing on blocks or
slides.  For this laboratory, the purchase of cassette and slide labelers
appeared to solve their needs.

On the other hand, if you'd like to address workflow issues within the
histology area, a solution with the appropriate printers and software is a
better choice.

The ultimate solution, in my opinion, is an implementation of printers and
software within the Anatomic Pathology LIS.  Such an implementation
addresses workflow issues not only within the histology area, but throughout
the department.  An example of this might be the tracking of slides as they
enter your stainer and an expected stain complete time viewable by a
pathologist in the same query screen as all other case data is displayed.

For many institutions, though, there is a constraint to use the existing LIS
and many existing LIS vendors do not incorporate an existing tracking or
workflow solution.  If this is the situation, then the next best choice is
the implementation of an 'add on' type solution.

May I suggest that since the annual NSH convention is just around the
corner, that you check out the vendor selections in Seattle.  Not only will
we be there demonstrating our LIS with a built in workflow and tracking
solution, but of course the other vendors will be there demonstrating their
wares.  Pick their brains, take what everyone says with a grain of salt, and
think about what you're really trying to achieve.  

If you'd like to pick my brain ahead of time, please feel free to email me
directly.



Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369
?
?
?


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perkocha,
Luke
Sent: Wednesday, July 21, 2010 10:37 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Vendors of Specimen Tracking Systems

Dear Listservers,

We're trying to compile a list of vendors of AP/histology specimen tracking
to investigate. We have Cerner Co-path LIS, and they have their own product.
The following are other vendors we've heard of - are their any others we
should investigate? Which of these are "not ready for prime time" in your
estimation?

    *   Ventana
    *   Dako
    *   Omni Trax
    *   Label Clinic
    *   ID positive, from General Data
    *   Brady Specimen Labeling
    *   University of Washington system - ? awaiting commercialization

Many thanks!

Luke Perkocha, MD, MBA
Associate Professor, Pathology and Dermatology
Associate Director, Surgical Pathology
University of California, San Francisco
Office: 415 885-7254


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From saby_joseph_a <@t> yahoo.com  Sun Jul 25 12:49:05 2010
From: saby_joseph_a <@t> yahoo.com (Joseph Saby)
Date: Sun Jul 25 12:49:10 2010
Subject: [Histonet] Artifacts in histology section
In-Reply-To: 
References: 
	
Message-ID: <431113.5115.qm@web114420.mail.gq1.yahoo.com>

What you are describing might be microchatter.? These will be sharp parallel 
lines/cracks that run parallel to the knife edge and are only visible under the 
microscope.

The usuall cause is a combination of overprocessing and rough facing that is too 
aggressive and/or with too dull a blade.? Overprocessing makes the tissue very 
hard and somewhat brittle.? The thick sections/dull knife cause the tissue to 
compress and then release, causing the chatter.? The actual danage is in the 
block face.

Once you have the problem in a block, if the tissue is thick enough, you might 
be able to repeatedly soak the block in ice water?and gently (with a fairly 
sharp knife) reface.? With luck, you might be able to get through the damaged 
block face.? 


Another artifact I have seen is similar, but the chatter appears very blurry.? 
This is usually caused be poor fixation/processing, then oversoaking the blocks 
after facing.? The trick here is to reface the block, then chill it without 
exposure to water.? I've sectioned such blocks after placing them in a freezer 
to chill them thoroughly.? This will help?to obtain?a section, but?may not fix 
the staining problems that?might show?up later.? 


Good luck!

Joe Saby, BA HT




________________________________
From: Aazath Raj 
To: histonet@lists.utsouthwestern.edu
Sent: Fri, July 23, 2010 11:26:28 AM
Subject: [Histonet] Artifacts in histology section



Dear Friends,

? ? ? ? ? ? ? I am an Histology Technologist. I am having a problem here,while 
sectioning am not seeing and scoring artifacts on the section but in the 
microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am 
not able to locate where is the problem,is that because of blades or due to 
micro-crystallization of wax or due to any processing problem. Its not 
consistently in all but i get it on some blocks every? day. Can any one help me 
in sorting it out. If anybody is interested in will send the picture of those 
section.





with regards,

Aazathraj.P

Technical Officer,

Apollo Hospitals-chennai

India.

aazath@hotmail.com


??? ??? ??? ? ??? ??? ? 
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From Steven.Weston <@t> utas.edu.au  Sun Jul 25 22:49:31 2010
From: Steven.Weston <@t> utas.edu.au (Steven Weston)
Date: Sun Jul 25 22:49:38 2010
Subject: [Histonet] Re water problem
Message-ID: 

We had this problem when we started using APTS coated slides and left out adhesive from the water bath. A simple solution I have found is to add a single drop of triton x100 (or similar detergent) to my full water bath and mix before heating the water. This reduces the surface tension of the water and allows it to run off the slide.
Regards
Steve weston
Menzies Research Institute
Hobart Tasmania, Australia
From TGoins <@t> mt.gov  Mon Jul 26 10:39:05 2010
From: TGoins <@t> mt.gov (Goins, Tresa)
Date: Mon Jul 26 10:39:16 2010
Subject: [Histonet] RE: Re water problem
In-Reply-To: 
References: 
Message-ID: 

Thanks for the great tip Steve - it works great!


Tresa Goins
Veterinary Diagnostic Lab
Department of Livestock
Bozeman, Montana



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Weston
Sent: Sunday, July 25, 2010 9:50 PM
To: histonet list
Subject: [Histonet] Re water problem

We had this problem when we started using APTS coated slides and left out adhesive from the water bath. A simple solution I have found is to add a single drop of triton x100 (or similar detergent) to my full water bath and mix before heating the water. This reduces the surface tension of the water and allows it to run off the slide.
Regards
Steve weston
Menzies Research Institute
Hobart Tasmania, Australia
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From Kim.Donadio <@t> bhcpns.org  Mon Jul 26 10:57:58 2010
From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org)
Date: Mon Jul 26 10:58:07 2010
Subject: [Histonet] Artifacts in histology section
In-Reply-To: <431113.5115.qm@web114420.mail.gq1.yahoo.com>
Message-ID: 

It's very difficult to diagnosis a problem such as this without hands on. 
But one thing that I noticed years ago that was so simple could cause 
this. A build up of tissues on the back of the blade holder. Worth a look. 





Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



Joseph Saby  
Sent by: histonet-bounces@lists.utsouthwestern.edu
07/25/2010 12:49 PM

To
Aazath Raj , histonet@lists.utsouthwestern.edu
cc

Subject
Re: [Histonet] Artifacts in histology section






What you are describing might be microchatter.  These will be sharp 
parallel 
lines/cracks that run parallel to the knife edge and are only visible 
under the 
microscope.

The usuall cause is a combination of overprocessing and rough facing that 
is too 
aggressive and/or with too dull a blade.  Overprocessing makes the tissue 
very 
hard and somewhat brittle.  The thick sections/dull knife cause the tissue 
to 
compress and then release, causing the chatter.  The actual danage is in 
the 
block face.

Once you have the problem in a block, if the tissue is thick enough, you 
might 
be able to repeatedly soak the block in ice water and gently (with a 
fairly 
sharp knife) reface.  With luck, you might be able to get through the 
damaged 
block face.  


Another artifact I have seen is similar, but the chatter appears very 
blurry.  
This is usually caused be poor fixation/processing, then oversoaking the 
blocks 
after facing.  The trick here is to reface the block, then chill it 
without 
exposure to water.  I've sectioned such blocks after placing them in a 
freezer 
to chill them thoroughly.  This will help to obtain a section, but may not 
fix 
the staining problems that might show up later.  


Good luck!

Joe Saby, BA HT




________________________________
From: Aazath Raj 
To: histonet@lists.utsouthwestern.edu
Sent: Fri, July 23, 2010 11:26:28 AM
Subject: [Histonet] Artifacts in histology section



Dear Friends,

              I am an Histology Technologist. I am having a problem 
here,while 
sectioning am not seeing and scoring artifacts on the section but in the 
microscope am seeing a tearing artifacts particularly in endoscopy 
biopsies. am 
not able to locate where is the problem,is that because of blades or due 
to 
micro-crystallization of wax or due to any processing problem. Its not 
consistently in all but i get it on some blocks every  day. Can any one 
help me 
in sorting it out. If anybody is interested in will send the picture of 
those 
section.





with regards,

Aazathraj.P

Technical Officer,

Apollo Hospitals-chennai

India.

aazath@hotmail.com


                        
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From arvidsonkristen <@t> yahoo.com  Mon Jul 26 11:07:57 2010
From: arvidsonkristen <@t> yahoo.com (kristen arvidson)
Date: Mon Jul 26 11:08:02 2010
Subject: [Histonet] Special Stain Storage
Message-ID: <820784.89949.qm@web65707.mail.ac4.yahoo.com>

Hello All,
We were recently inspected by CLIA and our inspector noticed that we didn't have reagent storage temperatures written in our Special Stains procedures.? We do our stains by hand so we do have some stored in the refrigerator and others stored at room temp.? I went through some of the Histo books and I cannot find any specifics on storage of reagents.? Any suggestions??
?
Thanks!!
Kristen


      
From tpodawiltz <@t> lrgh.org  Mon Jul 26 11:53:38 2010
From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas)
Date: Mon Jul 26 11:53:44 2010
Subject: [Histonet] Special Stain Storage
In-Reply-To: <820784.89949.qm@web65707.mail.ac4.yahoo.com>
References: <820784.89949.qm@web65707.mail.ac4.yahoo.com>
Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5DF832B@LRGHEXVS1.practice.lrgh.org>

That is a new one on me. It has never come up during any of our inspections. 

 
Tom Podawiltz HT (ASCP) 
Histology Section Head/Laboratory Safety Officer
LRGHealthcare




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson
Sent: Monday, July 26, 2010 12:08 PM
To: histonet
Subject: [Histonet] Special Stain Storage

Hello All,
We were recently inspected by CLIA and our inspector noticed that we didn't have reagent storage temperatures written in our Special Stains procedures.? We do our stains by hand so we do have some stored in the refrigerator and others stored at room temp.? I went through some of the Histo books and I cannot find any specifics on storage of reagents.? Any suggestions??
?
Thanks!!
Kristen


      
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From Bill.Tench <@t> pph.org  Mon Jul 26 12:16:36 2010
From: Bill.Tench <@t> pph.org (Tench, Bill)
Date: Mon Jul 26 12:16:42 2010
Subject: [Histonet] special stain storage
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A548E@MAIL1.pph.local>

The critical issue that your CLIA, or perhaps CAP, inspector is going to
be asking is:  is it necessary to storage stain reagent X in a
refrigerator with a temperature range of Y.  That answer lies in your
procedure.  If it says refrigerate (perhaps with a range) reagent X,
then you must document that you have done so by tracking the frig
temperature. If it really is not necessary to refrigerate the reagent
(perhaps it is done so just to keep it from evaporating) indicate that
in the procedure.
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

[None] made the following annotations
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From foreightl <@t> gmail.com  Mon Jul 26 12:27:17 2010
From: foreightl <@t> gmail.com (Pat Laurie)
Date: Mon Jul 26 12:27:24 2010
Subject: [Histonet] Powdered reagent expiration dates
Message-ID: 

We were inspected by CAP on friday and we were cited for


ANP.21366  *Are reagents and solutions properly labeled, as applicable and
appropriate, with the following elements?*

* *

1.      *Content and quantity, concentration or titer*

2.      *Storage requirements*

3.      *Date prepared or reconstituted by laboratory*

4.      *Expiration date*

Specifically that our staining powders didn't have an expiration date
printed on the bottle. All of our reconsituted reagents which are in
use were dated with an expiration date properly though. I have always
assumed, perhaphs incorrectly, that powdered stains never expire.  We have
powders like Luxol Echt Blau, etc. that were purchased and opened over 40
years ago.    If so, then these powdered reagents have gone through CAP
inspections since the beginning and this inspector was the first one to find
this problem.  Is this one that we might protest?

-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie@cellnetix.com
From Bill.Tench <@t> pph.org  Mon Jul 26 12:38:22 2010
From: Bill.Tench <@t> pph.org (Tench, Bill)
Date: Mon Jul 26 12:38:28 2010
Subject: [Histonet] powder stain expiration
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A548F@MAIL1.pph.local>

If the manufacturer has not included an expiration date for these, i
think there probably isn't one.  I would call the CAP LAP folks and ask
them about that.  When this kind of issue arises during an inspection,
it is always better to call while the inspectors are still there.  You
can get an answer right then and there and it will save everyone a lot
of time and hassle.
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

[None] made the following annotations
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 and may contain confidential and privileged information. Any unauthorized 
 review, use, disclosure or distribution is prohibited. If you are not the 
 intended recipient, please contact the sender by reply email and destroy all 
 copies of the original message. 

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From amario3 <@t> uic.edu  Mon Jul 26 12:53:50 2010
From: amario3 <@t> uic.edu (Andrea Marion)
Date: Mon Jul 26 12:53:57 2010
Subject: [Histonet] Re: Phospho-Histone 3 (SER10) antibody
Message-ID: <80dbbed564429fb4d4bcfff7da1e7042.squirrel@webmail.uic.edu>

I have used a phospho-H3 Ser10 antibody successfully on FFPE sections of
adult mouse heart and mouse embryos. Ours is Millipore 06-570 (formerly
Upstate 06-570) Lot# DAM1644557 and HIER with 10mM sodium citrate buffer,
0.05% Tween-20 pH 6.0 works great.

In the past we tried a phospho H3 Ser10 antibody from Cell Signaling
Technology #9706 and it works fine on Western blots, but we could never
get it to work on immunostainings. This is a different antibody and
company from Millipore/Upstate.

Andrea Marion
Graduate Student
University of Illinois - Chicago
amario3 [at] uic [dot] edu



I have been trying without success to get this antibody to work.   Is
anyone doing this antibody?  We have the Leica Bond Max and would like
to perform it on this platform.  I have tried 2 antibodies from Cell
Signaling Technology without any success.   Any advice would be greatly
appreciated.

Thanks!

Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
336-716-2104






From bill501 <@t> mindspring.com  Mon Jul 26 12:58:49 2010
From: bill501 <@t> mindspring.com (Bill B.)
Date: Mon Jul 26 12:58:54 2010
Subject: [Histonet] powder stain expiration
In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A548F@MAIL1.pph.local>
References: <2820431BF953BB4DA3E9E1A5882265FD034A548F@MAIL1.pph.local>
Message-ID: 

We verify these on control tissues yearly and keep a record whether or not there is an expiration date. I see no reason to throw away good material and waste yet more medical dollars. 

Bill Blank, MD

At 10:38 AM -0700 7/26/10, Tench, Bill wrote:
>If the manufacturer has not included an expiration date for these, i
>think there probably isn't one.  I would call the CAP LAP folks and ask
>them about that.  When this kind of issue arises during an inspection,
>it is always better to call while the inspectors are still there.  You
>can get an answer right then and there and it will save everyone a lot
>of time and hassle.


From DKBoyd <@t> chs.net  Mon Jul 26 13:01:23 2010
From: DKBoyd <@t> chs.net (DKBoyd@chs.net)
Date: Mon Jul 26 13:01:31 2010
Subject: [Histonet] Powdered reagent expiration dates
In-Reply-To: 
Message-ID: 

Some of our stains are very old also, about 10 years ago we wrote on a 
label on each stain; Opened prior to: with that days date and Stable.  We 
were CAP inspected many times after that and we had no problems.  Just as 
you would label your reagents you make up as Stable when there isn't an 
expiration time period.


Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd@chs.net







Pat Laurie  
Sent by: histonet-bounces@lists.utsouthwestern.edu
07/26/2010 01:27 PM

To
Histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] Powdered reagent expiration dates






We were inspected by CAP on friday and we were cited for


ANP.21366  *Are reagents and solutions properly labeled, as applicable and
appropriate, with the following elements?*

* *

1.      *Content and quantity, concentration or titer*

2.      *Storage requirements*

3.      *Date prepared or reconstituted by laboratory*

4.      *Expiration date*

Specifically that our staining powders didn't have an expiration date
printed on the bottle. All of our reconsituted reagents which are in
use were dated with an expiration date properly though. I have always
assumed, perhaphs incorrectly, that powdered stains never expire.  We have
powders like Luxol Echt Blau, etc. that were purchased and opened over 40
years ago.    If so, then these powdered reagents have gone through CAP
inspections since the beginning and this inspector was the first one to 
find
this problem.  Is this one that we might protest?

-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie@cellnetix.com
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From sareenprashant <@t> hotmail.com  Mon Jul 26 13:38:07 2010
From: sareenprashant <@t> hotmail.com (prashant sareen)
Date: Mon Jul 26 13:38:15 2010
Subject: [Histonet] RE: Histonet Digest, Vol 80, Issue 30
In-Reply-To: 
References: 
Message-ID: 


Try reducing the processing time and try very fine cutting and I think the microchatter will go.

 

 



Thanks
Prashant Sareen BS , M.B.A , HT (ASCP)
Associate Scientist III
Comprehensive Animal health Services
Bioreliance Corporation
14930 Broschart Road,

Rockville, MD 20850
Office: 301-610-2744
Histology: 301-610-2997

Cell: 240-315-5541
Email: prashant.sareen@bioreliance.com
 > From: histonet-request@lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 80, Issue 30
> To: histonet@lists.utsouthwestern.edu
> Date: Mon, 26 Jul 2010 10:02:53 -0700
> 
> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request@lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
> histonet-owner@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
> 1. Re: Artifacts in histology section (Joseph Saby)
> 2. Re water problem (Steven Weston)
> 3. RE: Re water problem (Goins, Tresa)
> 4. Re: Artifacts in histology section (Kim.Donadio@bhcpns.org)
> 5. Special Stain Storage (kristen arvidson)
> 6. RE: Special Stain Storage (Podawiltz, Thomas)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Sun, 25 Jul 2010 10:49:05 -0700 (PDT)
> From: Joseph Saby 
> Subject: Re: [Histonet] Artifacts in histology section
> To: Aazath Raj , histonet@lists.utsouthwestern.edu
> Message-ID: <431113.5115.qm@web114420.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
> 
> What you are describing might be microchatter.  These will be sharp parallel 
> lines/cracks that run parallel to the knife edge and are only visible under the 
> microscope.
> 
> The usuall cause is a combination of overprocessing and rough facing that is too 
> aggressive and/or with too dull a blade.  Overprocessing makes the tissue very 
> hard and somewhat brittle.  The thick sections/dull knife cause the tissue to 
> compress and then release, causing the chatter.  The actual danage is in the 
> block face.
> 
> Once you have the problem in a block, if the tissue is thick enough, you might 
> be able to repeatedly soak the block in ice water and gently (with a fairly 
> sharp knife) reface.  With luck, you might be able to get through the damaged 
> block face.  
> 
> 
> Another artifact I have seen is similar, but the chatter appears very blurry.  
> This is usually caused be poor fixation/processing, then oversoaking the blocks 
> after facing.  The trick here is to reface the block, then chill it without 
> exposure to water.  I've sectioned such blocks after placing them in a freezer 
> to chill them thoroughly.  This will help to obtain a section, but may not fix 
> the staining problems that might show up later.  
> 
> 
> Good luck!
> 
> Joe Saby, BA HT
> 
> 
> 
> 
> ________________________________
> From: Aazath Raj 
> To: histonet@lists.utsouthwestern.edu
> Sent: Fri, July 23, 2010 11:26:28 AM
> Subject: [Histonet] Artifacts in histology section
> 
> 
> 
> Dear Friends,
> 
>               I am an Histology Technologist. I am having a problem here,while 
> sectioning am not seeing and scoring artifacts on the section but in the 
> microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am 
> not able to locate where is the problem,is that because of blades or due to 
> micro-crystallization of wax or due to any processing problem. Its not 
> consistently in all but i get it on some blocks every  day. Can any one help me 
> in sorting it out. If anybody is interested in will send the picture of those 
> section.
> 
> 
> 
> 
> 
> with regards,
> 
> Aazathraj.P
> 
> Technical Officer,
> 
> Apollo Hospitals-chennai
> 
> India.
> 
> aazath@hotmail.com
> 
> 
>                         
> _________________________________________________________________
> The latest in fashion and style in MSN Lifestyle
> http://lifestyle.in.msn.com/_______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Mon, 26 Jul 2010 13:49:31 +1000
> From: Steven Weston 
> Subject: [Histonet] Re water problem
> To: histonet list 
> Message-ID: 
> Content-Type: text/plain; charset="iso-8859-1"
> 
> We had this problem when we started using APTS coated slides and left out adhesive from the water bath. A simple solution I have found is to add a single drop of triton x100 (or similar detergent) to my full water bath and mix before heating the water. This reduces the surface tension of the water and allows it to run off the slide.
> Regards
> Steve weston
> Menzies Research Institute
> Hobart Tasmania, Australia
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Mon, 26 Jul 2010 15:39:05 +0000
> From: "Goins, Tresa" 
> Subject: [Histonet] RE: Re water problem
> To: Steven Weston 
> Cc: "Histonet@lists.utsouthwestern.edu"
> 
> Message-ID:
> 
> Content-Type: text/plain; charset="us-ascii"
> 
> Thanks for the great tip Steve - it works great!
> 
> 
> Tresa Goins
> Veterinary Diagnostic Lab
> Department of Livestock
> Bozeman, Montana
> 
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Weston
> Sent: Sunday, July 25, 2010 9:50 PM
> To: histonet list
> Subject: [Histonet] Re water problem
> 
> We had this problem when we started using APTS coated slides and left out adhesive from the water bath. A simple solution I have found is to add a single drop of triton x100 (or similar detergent) to my full water bath and mix before heating the water. This reduces the surface tension of the water and allows it to run off the slide.
> Regards
> Steve weston
> Menzies Research Institute
> Hobart Tasmania, Australia
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Mon, 26 Jul 2010 10:57:58 -0500
> From: Kim.Donadio@bhcpns.org
> Subject: Re: [Histonet] Artifacts in histology section
> To: Joseph Saby 
> Cc: histonet@lists.utsouthwestern.edu, Aazath Raj
> , histonet-bounces@lists.utsouthwestern.edu
> Message-ID:
> 
> Content-Type: text/plain; charset="US-ASCII"
> 
> It's very difficult to diagnosis a problem such as this without hands on. 
> But one thing that I noticed years ago that was so simple could cause 
> this. A build up of tissues on the back of the blade holder. Worth a look. 
> 
> 
> 
> 
> 
> Kim Donadio 
> Pathology Supervisor
> Baptist Hospital
> 1000 W Moreno St.
> Pensacola FL 32501
> Phone (850) 469-7718
> Fax (850) 434-4996
> 
> 
> 
> Joseph Saby  
> Sent by: histonet-bounces@lists.utsouthwestern.edu
> 07/25/2010 12:49 PM
> 
> To
> Aazath Raj , histonet@lists.utsouthwestern.edu
> cc
> 
> Subject
> Re: [Histonet] Artifacts in histology section
> 
> 
> 
> 
> 
> 
> What you are describing might be microchatter. These will be sharp 
> parallel 
> lines/cracks that run parallel to the knife edge and are only visible 
> under the 
> microscope.
> 
> The usuall cause is a combination of overprocessing and rough facing that 
> is too 
> aggressive and/or with too dull a blade. Overprocessing makes the tissue 
> very 
> hard and somewhat brittle. The thick sections/dull knife cause the tissue 
> to 
> compress and then release, causing the chatter. The actual danage is in 
> the 
> block face.
> 
> Once you have the problem in a block, if the tissue is thick enough, you 
> might 
> be able to repeatedly soak the block in ice water and gently (with a 
> fairly 
> sharp knife) reface. With luck, you might be able to get through the 
> damaged 
> block face. 
> 
> 
> Another artifact I have seen is similar, but the chatter appears very 
> blurry. 
> This is usually caused be poor fixation/processing, then oversoaking the 
> blocks 
> after facing. The trick here is to reface the block, then chill it 
> without 
> exposure to water. I've sectioned such blocks after placing them in a 
> freezer 
> to chill them thoroughly. This will help to obtain a section, but may not 
> fix 
> the staining problems that might show up later. 
> 
> 
> Good luck!
> 
> Joe Saby, BA HT
> 
> 
> 
> 
> ________________________________
> From: Aazath Raj 
> To: histonet@lists.utsouthwestern.edu
> Sent: Fri, July 23, 2010 11:26:28 AM
> Subject: [Histonet] Artifacts in histology section
> 
> 
> 
> Dear Friends,
> 
> I am an Histology Technologist. I am having a problem 
> here,while 
> sectioning am not seeing and scoring artifacts on the section but in the 
> microscope am seeing a tearing artifacts particularly in endoscopy 
> biopsies. am 
> not able to locate where is the problem,is that because of blades or due 
> to 
> micro-crystallization of wax or due to any processing problem. Its not 
> consistently in all but i get it on some blocks every day. Can any one 
> help me 
> in sorting it out. If anybody is interested in will send the picture of 
> those 
> section.
> 
> 
> 
> 
> 
> with regards,
> 
> Aazathraj.P
> 
> Technical Officer,
> 
> Apollo Hospitals-chennai
> 
> India.
> 
> aazath@hotmail.com
> 
> 
> 
> _________________________________________________________________
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> http://lifestyle.in.msn.com/_______________________________________________
> 
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> 
> 
> 
> 
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
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> ------------------------------
> 
> Message: 5
> Date: Mon, 26 Jul 2010 09:07:57 -0700 (PDT)
> From: kristen arvidson 
> Subject: [Histonet] Special Stain Storage
> To: histonet 
> Message-ID: <820784.89949.qm@web65707.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
> 
> Hello All,
> We were recently inspected by CLIA and our inspector noticed that we didn't have reagent storage temperatures written in our Special Stains procedures.  We do our stains by hand so we do have some stored in the refrigerator and others stored at room temp.  I went through some of the Histo books and I cannot find any specifics on storage of reagents.  Any suggestions??
>  
> Thanks!!
> Kristen
> 
> 
> 
> 
> ------------------------------
> 
> Message: 6
> Date: Mon, 26 Jul 2010 12:53:38 -0400
> From: "Podawiltz, Thomas" 
> Subject: RE: [Histonet] Special Stain Storage
> To: 'kristen arvidson' , histonet
> 
> Message-ID:
> <38667E7FB77ECD4E91BFAEB8D986386323D5DF832B@LRGHEXVS1.practice.lrgh.org>
> 
> Content-Type: text/plain; charset="iso-8859-1"
> 
> That is a new one on me. It has never come up during any of our inspections. 
> 
> 
> Tom Podawiltz HT (ASCP) 
> Histology Section Head/Laboratory Safety Officer
> LRGHealthcare
> 
> 
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson
> Sent: Monday, July 26, 2010 12:08 PM
> To: histonet
> Subject: [Histonet] Special Stain Storage
> 
> Hello All,
> We were recently inspected by CLIA and our inspector noticed that we didn't have reagent storage temperatures written in our Special Stains procedures.  We do our stains by hand so we do have some stored in the refrigerator and others stored at room temp.  I went through some of the Histo books and I cannot find any specifics on storage of reagents.  Any suggestions??
>  
> Thanks!!
> Kristen
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> 
> 
> 
> ------------------------------
> 
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> 
> End of Histonet Digest, Vol 80, Issue 30
> ****************************************
 		 	   		  
From akbitting <@t> geisinger.edu  Mon Jul 26 13:39:07 2010
From: akbitting <@t> geisinger.edu (Angela Bitting)
Date: Mon Jul 26 13:39:26 2010
Subject: [Histonet] Powdered reagent expiration dates
In-Reply-To: 
References: 
Message-ID: <4C4D9E0C.2B7F.00C9.0@geisinger.edu>

Coincidentally, I had a question on this subject this morning. We rec'd in some oxalic acid and phoshotungstic acid, neither of which had expiration dates. I called Fisher and they looked it up for me and told me their expiration and offered to send it to me in writing, if I wanted. (it was 3-5 years from the manufacture date.) I was recently told by another tech that she thought 5 years was the shelf life of powders, but I wanted to see if that held true. Moral of the story is that it never hurts to call the manufacturer.

Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!


>>> Pat Laurie  7/26/2010 1:27 PM >>>
We were inspected by CAP on friday and we were cited for


ANP.21366  *Are reagents and solutions properly labeled, as applicable and
appropriate, with the following elements?*

* *

1.      *Content and quantity, concentration or titer*

2.      *Storage requirements*

3.      *Date prepared or reconstituted by laboratory*

4.      *Expiration date*

Specifically that our staining powders didn't have an expiration date
printed on the bottle. All of our reconsituted reagents which are in
use were dated with an expiration date properly though. I have always
assumed, perhaphs incorrectly, that powdered stains never expire.  We have
powders like Luxol Echt Blau, etc. that were purchased and opened over 40
years ago.    If so, then these powdered reagents have gone through CAP
inspections since the beginning and this inspector was the first one to find
this problem.  Is this one that we might protest?

-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie@cellnetix.com 
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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-------------- next part --------------
BEGIN:VCARD
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X-GWTYPE:USER
FN:Bitting, Angela
TEL;WORK:570-271-6844
ORG:;Histology
EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu
N:Bitting;Angela
END:VCARD

From denise.woodward <@t> uconn.edu  Mon Jul 26 14:50:25 2010
From: denise.woodward <@t> uconn.edu (Woodward, Denise)
Date: Mon Jul 26 14:50:33 2010
Subject: [Histonet] Re: Special Stain Storage
Message-ID: 

Hi All,
In Charles J Churukian's  "Manual of the Special Stains Laboratory of the Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY"  pg 192, he suggests "the following chemicals should be kept refrigerated":  all buffer solutions, hydroquinone, Phosphomolybdic acid, Phosphotungstic acid, Protargol, silver nitrate and all solutions containing silver nitrate.   This information is under the section labeled "Stability of Special Staining Solutions - General  guidelines Concerning Solutions."

Everywhere I have ever worked, we also kept the Schiff's reagent refrigerated for longer shelf life.

Regards,
Denise Long Woodward, MS, HT (ASCP) HTL,  QIHC
University of Connecticut

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson
Sent: Monday, July 26, 2010 12:08 PM
To: histonet
Subject: [Histonet] Special Stain Storage

Hello All,
We were recently inspected by CLIA and our inspector noticed that we didn't have reagent storage temperatures written in our Special Stains procedures.? We do our stains by hand so we do have some stored in the refrigerator and others stored at room temp.? I went through some of the Histo books and I cannot find any specifics on storage of reagents.? Any suggestions??
?
Thanks!!
Kristen



From Mark.Elliott <@t> hli.ubc.ca  Mon Jul 26 16:26:29 2010
From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott)
Date: Mon Jul 26 16:28:53 2010
Subject: [Histonet] Myocardium infected with influenza
In-Reply-To: <48B0C4C4.548@mail.mrl.ubc.ca>
References: <48B0C4C4.548@mail.mrl.ubc.ca>
Message-ID: <4C4D9B15020000D60004C926@mail.mrl.ubc.ca>

Does anyone have a block of myocardial tissue with a known influenza infection they can spare?  Even a few slides would be great.  
Thanks
Mark
 
 

***CONFIDENTIALITY NOTICE***
This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential.  Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system.

From lpwenk <@t> sbcglobal.net  Mon Jul 26 18:41:47 2010
From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk)
Date: Mon Jul 26 18:41:55 2010
Subject: [Histonet] Powdered reagent expiration dates
In-Reply-To: 
Message-ID: <7F1F706999AC4C57BA2B8AC8D15BB5A4@HPPav2>

See if you can get the following article. Biotech Histochem is published by
the Biological Stain Commission. http://www.biologicalstaincommission.org/

Biotech Histochem. 2009 Feb;84(1):11-5.

Stain and dye stability over a 30-year period: a comparison of certified dye
powders by the Biological Stain Commission.
Penney DP, Frank M, Fagan C, Willis C.

Biological Stain Commission, Department of Pathology & Laboratory Medicine,
University of Rochester Medical Center, Rochester, New York 14642-0001, USA.
David_Penney@urmc.rochester.edu

Abstract
The Biological Stain Commission (BSC) Assay Laboratory has received numerous
inquiries during the past several years regarding the long-term stability of
stain and dye powders, particularly since packaging requirements call for
expiration dates on reagents. We have conducted a study to examine the
long-term stability of selected dye powders. We used the standard procedures
of the BSC for testing biological stains for certification to give an
indication of the long-term chemical stability as well as staining
performance of the dye powders. An earlier study by Emmel and Stotz examined
the stability of various dye powders after a five-year storage period. The
present study is a follow-up project covering the same dyes after storage
for 30 years. The dye samples chosen for the study are the same samples used
in the five-year storage period study and give comparative results for all
three time periods. The results of this study affirm the generally held
speculation that dye powders are stable for many years and thus have a
substantial shelf-life. 

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie
Sent: Monday, July 26, 2010 1:27 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Powdered reagent expiration dates

We were inspected by CAP on friday and we were cited for


ANP.21366  *Are reagents and solutions properly labeled, as applicable and
appropriate, with the following elements?*

* *

1.      *Content and quantity, concentration or titer*

2.      *Storage requirements*

3.      *Date prepared or reconstituted by laboratory*

4.      *Expiration date*

Specifically that our staining powders didn't have an expiration date
printed on the bottle. All of our reconsituted reagents which are in use
were dated with an expiration date properly though. I have always assumed,
perhaphs incorrectly, that powdered stains never expire.  We have powders
like Luxol Echt Blau, etc. that were purchased and opened over 40
years ago.    If so, then these powdered reagents have gone through CAP
inspections since the beginning and this inspector was the first one to find
this problem.  Is this one that we might protest?

--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie@cellnetix.com
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From AnthonyH <@t> chw.edu.au  Tue Jul 27 02:24:07 2010
From: AnthonyH <@t> chw.edu.au (Tony Henwood)
Date: Tue Jul 27 02:24:41 2010
Subject: [Histonet] Formalin substitutes
In-Reply-To: <0A1C03FC4E63EE4AA24A7983E91C25B502938034@vla-exchn1.cvlnt.vla.gov.uk>
Message-ID: 

The following info might be useful:

It is believed that formalin given time will kill any microorganisms
that are present in tissue and that formalin will inactivate
tuberculosis.

Vardaxis et al (J Clin Pathol 1997;50:429-433) were quite rightly
concerned with the disinfection of bacterial endospores. Endospores can
survive the most adverse chemical and physical environments and can
cause such diseases as tetanus, anthrax, gas gangrene and botulism. 

They used autoclave spore containing test strips and fixed them in
various fixatives such as 10% neutral buffered formalin, ethanol in
various concentrations (70% & 50%) and two commercial non-formalin
fixatives (Kryofix & Spuitfix). Fixation times varied from 24 hours to
14 days.

They found that 10% formalin killed all microbes within 24 hours, where
as only one non-formalin fixative (Spuitfix) killed the spores and this
was only after 7 days fixation. Microwave fixation also did not kill the
spores.

These workers did note that spore strips do not behave like tissue and
that tissue may have a protective effect on pathogens.

Cleary et al (J Clin Pathol 2005;58:22-25) studied the antimicrobial
effects of UMFix, an alcohol based tissue fixative, on various
microorganisms. The UMFix solution was compared with 10% neutral
buffered formalin.

After a short exposure, UMFix rapidly killed vegetative bacteria,
yeasts, moulds, and viruses. Bacterial spores were resistant to killing
by UMFix. All organisms were killed by the 10% neutral buffered formalin
preparation.
They concluded that UMFix was microbicidal for vegetative bacteria,
yeasts, and aspergillus species after a short exposure, although it was
not active against spore forming bacillus species. The methanol content
of the fixative was responsible for the killing effect of this fixative.
No killing was seen when polyethylene glycol was used alone.

Kappel et al (HUM PATHOL 27:1361--1364, 1996) attempted to grow TB from
formalin fixed lung tissue that had previously been shown to be positive
by sputum culture. They were unable to culture TB from these tissues.

Gerston et al (HUM PATHOL 35:571-575.2004) in South Africa analysed 138
formalin fixed lungs with histological evidence of AFB and were able to
culture TB from 12 of these cases (one of these cases had been fixed for
80 days before being tested).

Gerston et al suggest that there is a risk of contracting tuberculosis
from tissue that has been fixed in formalin, if aerosols or accidental
inoculation should occur. 

Trimming and sectioning wax blocks are of concern but no studies have
been done yet.

Of concern to histotechnolgists are:
1.	Tissue with Inflammation-induced Encapsulation may protect bugs
from formalin.
2.	Formalin dilutes as it penetrates tissue.
3.	Formalin substitutes may not be germicidal.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weaver,
Colin
Sent: Saturday, 24 July 2010 2:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin substitutes


Hi everyone - does anyone know how effective these formalin substitutes
are at killing microorgansms9esp HG3) in the tissue fixed? I know that
Finefix states that because of the alcohol content being over 70% that
it is effective against many micro-organisms but info seems scarce on
the others

 

Thanks and have a nice weekend

 

Colin Weaver

Histology lab manager

VLA

Thirsk

North Yorkshire

UK

Veterinary Laboratories Agency (VLA)

This email and any attachments is intended for the named
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From bstephen <@t> fastmail.fm  Tue Jul 27 06:44:12 2010
From: bstephen <@t> fastmail.fm (Birgitta Stephenson)
Date: Tue Jul 27 06:44:19 2010
Subject: [Histonet] Reaction/s between Phloroglucinol and Toluidine Blue ?
Message-ID: <1280231052.30823.1386946451@webmail.messagingengine.com>

Hello Histonetters,

I have been staining residues with Phloroglucinol which is light
sensitive however I due to time constraints I have not been able to wait
for all the colour to fade ( as this takes about one week). Therefore, I
have dried off the stained residue under lights and then counterstained
with Toluidine Blue. This seems to be ok, with the Toluidine Blue
restaining the residue as expected. I was wondering if anyone knows of
any possible interaction with Phloroglucinol (in HCL) and Toluidine Blue
? Likewise the residue stained with Phloroglucinol and dried seems to
develop almost crystal like tracks that disappear when restained. I am
not sure if the "tracks" are due to Phloroglucinol reacting with light?
Any ideas welcomed.

Thank you

Birgitta Stephenson
Microscopy Research Lab, University of Queensland.
-- 
  Birgitta Stephenson
  bstephen@fastmail.fm

-- 
http://www.fastmail.fm - The way an email service should be


From schaundrawalton <@t> yahoo.com  Tue Jul 27 06:55:42 2010
From: schaundrawalton <@t> yahoo.com (Schaundra Walton)
Date: Tue Jul 27 06:55:46 2010
Subject: [Histonet] Job Opportunity
Message-ID: <500414.54374.qm@web120616.mail.ne1.yahoo.com>

Hey histonetters!
?
Keiser University is looking for a program coordinator for their histotechnology program at their Pembroke Pines campus.? For further information please contact 
?
Tania Phillips, MBA 
Associate Dean - Allied Health
Keiser University - Pembroke Pines 
12520 Pines Boulevard 
Pembroke Pines, FL 33027 
Phone: 954.431.4300 
Fax: 954.431.2929 
Website: http://www.keiseruniversity.edu 
Email: tphillips@keiseruniversity.edu


      
From Kimberly.Poole <@t> drdc-rddc.gc.ca  Tue Jul 27 09:27:58 2010
From: Kimberly.Poole <@t> drdc-rddc.gc.ca (Poole, Kimberly)
Date: Tue Jul 27 09:28:10 2010
Subject: [Histonet] Brain Problems
Message-ID: <42DFE1A029181B4B8CCBA7261B52D765013A77D4@suffieldex01.suffield.drdc-rddc.gc.ca>

Hi there,

 

I am currently using rat brain for histology but something is not working. I follow the protocol below for tissue processing.

 

Station

Solution

Routine (mins)

 

 

 

1

Formalin

10

2

Formalin

10

3

70% Ethanol

60

4

80% Ethanol

240

5

96% Ethanol

240

6

100% Ethanol

240

7

Xylene

120

8

Xylene

120

9

Xylene

120

10

Paraffin Wax

120

11

Paraffin Wax

180

12

Paraffin Wax

180

TOTAL TIME

 

27 hours 20 minutes

 

 

	

 

I embedded my brains and then cut them afterwards. When the brain section hits the water, it looks like a blob of fat on the water. Then it spreads reall, really slowly. If a cut is made to the paraffin the brain bursts open and it falls apart. Am I doing something wrong in the processing stage? Is the tissue dehydrated too much. Any feedback would be greatly appreciated.

Kimberly Poole B.Sc

Casualty Management Section | Section de la gestion des bless?s

Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield 

Medicine Hat, AB, Canada T1A 8K6

kimberly.poole@drdc-rddc.gc.ca  

Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 

Government of Canada | Gouvernement du Canada

From Loralee_Mcmahon <@t> URMC.Rochester.edu  Tue Jul 27 09:50:47 2010
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Tue Jul 27 09:54:55 2010
Subject: [Histonet] RE: Brain Problems
In-Reply-To: <42DFE1A029181B4B8CCBA7261B52D765013A77D4@suffieldex01.suffield.drdc-rddc.gc.ca>
References: <42DFE1A029181B4B8CCBA7261B52D765013A77D4@suffieldex01.suffield.drdc-rddc.gc.ca>
Message-ID: 

How big are these rat brains?  Are you processing by hand or with a machine?  
We routinely process pieces of human brain for about 10 to 11 hours on a VIP.  This seems like a really really long cycle.  No heat and no vacuum until the paraffin infiltration step. (formalin, 70% EtoH, 95% EtoH, 95% EtoH, 100% EtoH, 100% EtoH, Xylene, Xylene, paraffin, paraffin)
You might want to back it off to one hour in each step and then see what you get.  You may be able to get away with an even shorter cycle if the pieces of brain are smaller.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poole, Kimberly [Kimberly.Poole@drdc-rddc.gc.ca]
Sent: Tuesday, July 27, 2010 10:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Brain Problems

Hi there,



I am currently using rat brain for histology but something is not working. I follow the protocol below for tissue processing.



Station

Solution

Routine (mins)







1

Formalin

10

2

Formalin

10

3

70% Ethanol

60

4

80% Ethanol

240

5

96% Ethanol

240

6

100% Ethanol

240

7

Xylene

120

8

Xylene

120

9

Xylene

120

10

Paraffin Wax

120

11

Paraffin Wax

180

12

Paraffin Wax

180

TOTAL TIME



27 hours 20 minutes









I embedded my brains and then cut them afterwards. When the brain section hits the water, it looks like a blob of fat on the water. Then it spreads reall, really slowly. If a cut is made to the paraffin the brain bursts open and it falls apart. Am I doing something wrong in the processing stage? Is the tissue dehydrated too much. Any feedback would be greatly appreciated.

Kimberly Poole B.Sc

Casualty Management Section | Section de la gestion des bless?s

Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield

Medicine Hat, AB, Canada T1A 8K6

kimberly.poole@drdc-rddc.gc.ca 

Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714

Government of Canada | Gouvernement du Canada

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From sgoebel <@t> xbiotech.com  Tue Jul 27 10:01:03 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Tue Jul 27 10:01:09 2010
Subject: [Histonet] Brain Problems
Message-ID: <20100727080103.9e2d9aa830e8449a2412eb1e4f2f067e.9813e842e4.wbe@email04.secureserver.net>


   Try  cooling down your water bath with ice before you cut the brai   All  CNS  tissue  will  act like you described.  You may end up w   more  wrinkles  with the cooler bath, but put the slides in a 70C oven
   fo
   Happy Cuttin
   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician
   
   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 Suite 10
   Austin, Texas  78744

   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] Brain Problems
   From: "Poole, Kimberly" <[1]Kimberly.Poole@drdc-rddc.gc.ca>
   Date: Tue, July 27, 2010 7:27 am
   To: <[2]histonet@lis   Hi there,
   I  am  currently  using  rat  brain for histology but something is not
   working.    Station
   Solution
   Routine (mins)
   1
   Formalin
   10
   2
   Formalin
   10
   3
   70% Ethanol
   60
   4
   80% Ethanol
   240
   5
   96% Ethanol
   240
   6
   100% Ethanol
   240
   7
   Xylene
   120
   8
   Xylene
   120
   9
   Xylene
   120
   10
   Paraffin Wax
   120
   11
   Paraffin Wax
   180
   12
   Paraffin Wax
   180
   TOTAL TIME
   27 hours 20 minutes
   I  embedded  my  brains  and  then cut them afterwards. When the brain
   section  h   Then  it  spreads  re   paraffin  the  brain  bursts  open     something  wrong  in  the processing stage? Is    too much. Any feedback would be greatly appreciated.<   Kimberly Poole [3]B.Sc
   Casualty Management Section | Section de la gestion des bless?s
   Defence  Research  and  Development  Canada  Suffield  |  Recherche et
   d?velo   Medicine Hat, AB, Canada T1A 8K6
   [4]kimberly.poole@drdc-rdd   mberly.poole@drdc-rddc.gc.ca>
   Telephone   |   T?l?phone   403-544-5347   /   Facsimile   |
   T?l?co   Government of Canada | Gouvernement du Canada
   _______________________________________________
   Histonet mailing list
   [6]Histonet@lists.utsou   [7]http:
References

   1. 3D"mailto://Kimberly.Poole@drdc-rddc.gc=/
   2. 3D"mailto://histonet@lists.utsouthwestern.edu"/
   3. 3D"http://B.Sc"/
   4. 3D"mailto://kimberly.poole@drdc-rddc.gc.ca"/
   5. 3D"mailto:kimberly.poole@drdc-rddc.gc.ca"
   6. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
   7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
From MSHERWOOD <@t> PARTNERS.ORG  Tue Jul 27 10:14:05 2010
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Tue Jul 27 10:14:10 2010
Subject: [Histonet] Re:  [Sectioning]  Brain Tissue
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E244DE@PHSXMB30.partners.org>

To All:
 
We have been experiencing problems sectioning mouse brain.  The sectioning is
fine, but we have problems in the water bath.  At 45 degrees C (peel-a-way
paraffin-Polysciences), the sections break apart but we don't get wrinkles.
With paraplast extra, we use 36 degrees C, but the sections are wrinkled and
disintegrate.   
 
I would appreciate hearing from listers as to what you do:  what temperature is
your water bath, what embedding media do you use, etc?  Any help would be
appreciated!
 
Thanks!
Peggy
 
Peggy Sherwood
Lab Associate, Photopathology 
Wellman Center for Photomedicine (EDW 214)
Massachusetts General Hospital
55 Fruit Street
Boston, Massachusetts 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org
 


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From sgoebel <@t> xbiotech.com  Tue Jul 27 10:19:21 2010
From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com)
Date: Tue Jul 27 10:19:25 2010
Subject: [Histonet] Re: [Sectioning] Brain Tissue
Message-ID: <20100727081921.9e2d9aa830e8449a2412eb1e4f2f067e.f0c6539876.wbe@email04.secureserver.net>


   My water bath is usually around the same temperature as yours is.   just  take literally a hand full of ice and put it in the bath, onc   all  the ice melts, I cut the brain.  I'll try to go look at what tem   p  it  gets  to,  my  guess  would  be  around room temperature?  Your
   section
   <
   Sarah Goebel, B
   Histotechnician
   
   <
   XBiotech USA I
   8201 East Riverside Dr. Bldg 4 Suite 100

   
   Aust
   (512)386-5107

   
   -------- Original Message --------
   Subject: [Histonet] Re: [Sectioning] Brain Tissue
   From: "Sherwood, Margaret " <[1]MSHERWOOD@PARTNERS.ORG>
   Date: Tue, July 27, 2010 8:14 am
   To: <[2]histonet@lis   To All:
   We  have  been  experiencing  problems  sectioning  mouse  brain.  The
   sectioning    fine,  but  we  have  problems  in  the  water  bath.  At 45 degrees C
   (peel-a-way<   we don't get wrinkles.   With  paraplast  extra,  we  use  36  degrees  C, but the sections are
   wrinkled an   disintegrate.
   I  would  appreciate  hearing  from  listers  as  to what you do: what
   temperatur   your  water bath, what embedding media do you use, etc? Any help would
   be appreciated!
   Thanks!
   Peggy
   Peggy Sherwood
   Lab Associate, Photopathology
   Wellman Center for Photomedicine (EDW 214)
   Massachusetts General Hospital
   55 Fruit Street
   Boston, Massachusetts 02114-2696
   617-724-4839 (voice mail)
   617-726-6983 (lab)
   617-726-1206 (fax)
   [3]msherwood@partners.org
   The information in this e-mail is intended only for the person to whom
   it i   addressed. If you believe this e-mail was sent to you in error and the
   e-ma   contains  patient  information, please contact the Partners Compliance
   HelpLi   [4]http://www.partners.org/   to you in error
   but  does  not  contain patient information, please contact the sender
   and pro   dispose of the e-mail.
   _______________________________________________
   Histonet mailing list
   [5]Histonet@lists.utsou   [6]http:   

References

   1. 3D"mailto://MSHERWOOD@PARTNERS.ORG"=/
   2. 3D"mailto://histonet@lists.utsouthwestern.edu"/
   3. 3D"mailto://msherwood@partners.org"/
   4. 3D"http://www.partners.org/complianceline"
   5. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
   6. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
From Nancy.Herman <@t> inspection.gc.ca  Tue Jul 27 10:25:54 2010
From: Nancy.Herman <@t> inspection.gc.ca (Nancy Herman)
Date: Tue Jul 27 10:26:06 2010
Subject: [Histonet] Re:  [Sectioning]  Brain Tissue
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E244DE@PHSXMB30.partners.org>
References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E244DE@PHSXMB30.partners.org>
Message-ID: <4C4EA622.6DEB.00DD.0@inspection.gc.ca>

We keep our water bath at 40-41 C.  Sometimes we add some 70% ethanol to the water bath and this helps to get the wrinkles out (especially for cerebellum and mice brain).  Most of our tissue is bovine brain but we deal with multiple other species including mice.  We use McCormick Paraplast Plus embedding Medium from Fisher.

Nancy Herman
Canadian Food Inspection Agency - Lethbridge Laboratory

>>> "Sherwood, Margaret "  2010/07/27 9:14 am >>>
To All:
 
We have been experiencing problems sectioning mouse brain.  The sectioning is
fine, but we have problems in the water bath.  At 45 degrees C (peel-a-way
paraffin-Polysciences), the sections break apart but we don't get wrinkles.
With paraplast extra, we use 36 degrees C, but the sections are wrinkled and
disintegrate.   
 
I would appreciate hearing from listers as to what you do:  what temperature is
your water bath, what embedding media do you use, etc?  Any help would be
appreciated!
 
Thanks!
Peggy
 
Peggy Sherwood
Lab Associate, Photopathology 
Wellman Center for Photomedicine (EDW 214)
Massachusetts General Hospital
55 Fruit Street
Boston, Massachusetts 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org 
 


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From foreightl <@t> gmail.com  Tue Jul 27 11:00:20 2010
From: foreightl <@t> gmail.com (Pat Laurie)
Date: Tue Jul 27 11:00:30 2010
Subject: [Histonet] Powdered reagent expiration dates
In-Reply-To: <7F1F706999AC4C57BA2B8AC8D15BB5A4@HPPav2>
References: 
	<7F1F706999AC4C57BA2B8AC8D15BB5A4@HPPav2>
Message-ID: 

Everyone,

Thanks for your help, based off your explanations, statements and citations,
we will attempt to protest this and have it expunged.



On Mon, Jul 26, 2010 at 4:41 PM, Lee & Peggy Wenk wrote:

> See if you can get the following article. Biotech Histochem is published by
> the Biological Stain Commission. http://www.biologicalstaincommission.org/
>
> Biotech Histochem. 2009 Feb;84(1):11-5.
>
> Stain and dye stability over a 30-year period: a comparison of certified
> dye
> powders by the Biological Stain Commission.
> Penney DP, Frank M, Fagan C, Willis C.
>
> Biological Stain Commission, Department of Pathology & Laboratory Medicine,
> University of Rochester Medical Center, Rochester, New York 14642-0001,
> USA.
> David_Penney@urmc.rochester.edu
>
> Abstract
> The Biological Stain Commission (BSC) Assay Laboratory has received
> numerous
> inquiries during the past several years regarding the long-term stability
> of
> stain and dye powders, particularly since packaging requirements call for
> expiration dates on reagents. We have conducted a study to examine the
> long-term stability of selected dye powders. We used the standard
> procedures
> of the BSC for testing biological stains for certification to give an
> indication of the long-term chemical stability as well as staining
> performance of the dye powders. An earlier study by Emmel and Stotz
> examined
> the stability of various dye powders after a five-year storage period. The
> present study is a follow-up project covering the same dyes after storage
> for 30 years. The dye samples chosen for the study are the same samples
> used
> in the five-year storage period study and give comparative results for all
> three time periods. The results of this study affirm the generally held
> speculation that dye powders are stable for many years and thus have a
> substantial shelf-life.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> Beaumont Hospital
> Royal Oak, MI 48073
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie
> Sent: Monday, July 26, 2010 1:27 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Powdered reagent expiration dates
>
>  We were inspected by CAP on friday and we were cited for
>
>
> ANP.21366  *Are reagents and solutions properly labeled, as applicable and
> appropriate, with the following elements?*
>
> * *
>
> 1.      *Content and quantity, concentration or titer*
>
> 2.      *Storage requirements*
>
> 3.      *Date prepared or reconstituted by laboratory*
>
> 4.      *Expiration date*
>
> Specifically that our staining powders didn't have an expiration date
> printed on the bottle. All of our reconsituted reagents which are in use
> were dated with an expiration date properly though. I have always assumed,
> perhaphs incorrectly, that powdered stains never expire.  We have powders
> like Luxol Echt Blau, etc. that were purchased and opened over 40
> years ago.    If so, then these powdered reagents have gone through CAP
> inspections since the beginning and this inspector was the first one to
> find
> this problem.  Is this one that we might protest?
>
> --
> Patrick Laurie HT(ASCP)QIHC
> CellNetix Pathology & Laboratories
> 1124 Columbia Street, Suite 200
> Seattle, WA 98104
> PH: 206-215-5949
> plaurie@cellnetix.com
>  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie@cellnetix.com
From abijag76 <@t> rediffmail.com  Tue Jul 27 10:30:26 2010
From: abijag76 <@t> rediffmail.com (abijag )
Date: Tue Jul 27 11:31:01 2010
Subject: [Histonet] Tissue processing protocol for Hamster tissues
Message-ID: <20100727153026.31966.qmail@f6mail-144-201.rediffmail.com>

Dear Histonetters,

We were asked to process Hamster tissue(Large intestine) for one of our investigators. I would like to know whether any of our users process hamster tissues in their labs, if so kindly provide me a protocol for the same. Our tissue processor is Sakura VIP6.



Thanks for all



Abi jagannath
From algranth <@t> email.arizona.edu  Tue Jul 27 12:32:18 2010
From: algranth <@t> email.arizona.edu (Andrea Grantham)
Date: Tue Jul 27 12:32:25 2010
Subject: [Histonet] question for a friend
Message-ID: 


A friend is asking this question and we were looking for responses  
telling what others would do in this situation:


  I am hoping to submit some brains for paraffin-embedded sectioning
soon, but we started intending to go the frozen-section route, so I
have some questions as to the feasibility of paraffin-embedding with
our storage conditions.  We began by fixing our brain-tissue with
paraformaldehyde (transcardial-perfusion).  Then, we stored in
paraformaldehyde for 4 hours before switching to 10% sucrose
overnight.  The next day we switched to 30% sucrose and stored
overnight (both sucrose storage at 4 degrees Celsius).  The brains
were then switched over to a cryoprotectant (which contains PBS,
sucrose, polyvinylpyrrolidone, and ethylene glycol) and stored at -20
degrees Celsius.  The brains have been in the cryoprotectant for
about a week, now.  Do you see anything in these storage conditions
that would prevent you from being able to paraffin-embed and section
these brains?  Thank you much.



Thanks,
Andi Grantham





Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth@email.arizona.edu
Tel: 520.626.4415     Fax: 520.626.2097

"happy slicing and dicing and may all your stains work perfectly" -  
Paula Sicurello
P Please consider the environment before printing this email.




From Randi.Hayes <@t> horizonnb.ca  Tue Jul 27 13:03:33 2010
From: Randi.Hayes <@t> horizonnb.ca (Hayes, Randi (HorizonNB))
Date: Tue Jul 27 13:03:31 2010
Subject: [Histonet] Using GEWF solution and IHC staining
Message-ID: 

At a recent conference, our PA learned of using GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph Node retrieval in Colorectal Cancer resections.  Although a good idea, I'm wondering how "safe" it is to use when staining for IHC.  Does anyone have much experience with this or know of a study (studies) that have been done to verify that the ethanol is not destroying antigen sites?  We're a little concerned......


Randi Hayes, MLT
Histology Supervisor / Superviseur d'Histologie
Horizon Health Network / R?seau de sant? Horizon
(506) 860-2157
randi.hayes@HorizonNB.ca  
www.HorizonNB.ca
------- Horizon Health Network Disclaimer -------

This e-mail communication (including any or all attachments) is intended
only for the use of the person or entity to which it is addressed and may
contain confidential and/or privileged material. If you are not the intended
recipient of this e-mail, any use, review, retransmission, distribution,
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reliance upon this e-mail, is strictly prohibited. If you have received this
e-mail in error, please contact the sender and delete the original and any
copy of this e-mail and any printout thereof, immediately. Your
co-operation is appreciated.

Le pr?sent courriel (y compris toute pi?ce jointe) s'adresse uniquement ?
son destinataire, qu'il soit une personne ou un organisme, et pourrait
comporter des renseignements privil?gi?s ou confidentiels. Si vous n'?tes
pas le destinataire du courriel, il est interdit d'utiliser, de revoir, de
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courriel, d'agir en vous y fiant ou de vous en servir de toute autre fa?on.
Si vous avez re?u le pr?sent courriel par erreur, pri?re de communiquer
avec l'exp?diteur et d'?liminer l'original du courriel, ainsi que toute copie
?lectronique ou imprim?e de celui-ci, imm?diatement. Nous sommes
reconnaissants de votre collaboration.
From foreightl <@t> gmail.com  Tue Jul 27 14:07:44 2010
From: foreightl <@t> gmail.com (Patrick Laurie)
Date: Tue Jul 27 14:07:48 2010
Subject: [Histonet] Using GEWF solution and IHC staining
In-Reply-To: 
References: 
Message-ID: 

Sounds like to me that it might involve another revalidation of each one of
your antibodies.

Good luck

On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB) <
Randi.Hayes@horizonnb.ca> wrote:

> At a recent conference, our PA learned of using GEWF (glacial acetic acid,
> ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph
> Node retrieval in Colorectal Cancer resections.  Although a good idea, I'm
> wondering how "safe" it is to use when staining for IHC.  Does anyone have
> much experience with this or know of a study (studies) that have been done
> to verify that the ethanol is not destroying antigen sites?  We're a little
> concerned......
>
>
> Randi Hayes, MLT
> Histology Supervisor / Superviseur d'Histologie
> Horizon Health Network / R?seau de sant? Horizon
> (506) 860-2157
> randi.hayes@HorizonNB.ca 
> www.HorizonNB.ca  
>
>
>
>
------- Horizon Health Network Disclaimer -------

This e-mail > communication (including any or all attachments) is intended
only for the > use of the person or entity to which it is addressed and may
contain > confidential and/or privileged material. If you are not the > intended
recipient of this e-mail, any use, review, retransmission, > distribution,
dissemination, copying, printing, or other use of, or > taking of any action in
reliance upon this e-mail, is strictly > prohibited. If you have received this
e-mail in error, please contact the > sender and delete the original and any
copy of this e-mail and any > printout thereof, immediately. Your
co-operation is > appreciated.

Le pr?sent courriel (y compris toute pi?ce jointe) > s'adresse uniquement ?
son destinataire, qu'il soit une personne ou un > organisme, et pourrait
comporter des renseignements privil?gi?s ou > confidentiels. Si vous n'?tes
pas le destinataire du courriel, il est > interdit d'utiliser, de revoir, de
retransmettre, de distribuer, de > diss?miner, de copier ou d'imprimer ce
courriel, d'agir en vous y fiant > ou de vous en servir de toute autre fa?on.
Si vous avez re?u le pr?sent > courriel par erreur, pri?re de communiquer
avec l'exp?diteur et > d'?liminer l'original du courriel, ainsi que toute copie
?lectronique ou > imprim?e de celui-ci, imm?diatement. Nous sommes
reconnaissants de votre > collaboration.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie@cellnetix.com
From mhale <@t> carisls.com  Tue Jul 27 14:40:42 2010
From: mhale <@t> carisls.com (Hale, Meredith)
Date: Tue Jul 27 14:40:49 2010
Subject: [Histonet] Great New Position In Irving, Texas 
Message-ID: <6F33D8418806044682A391273399860F049FD112@s-irv-ex301.PathologyPartners.intranet>

 

 

Nueterra, a leading developer and manager of physician-owned surgery
centers and specialty hospitals has an immediate opportunity available
for a Histotechnician.

 

Great opportunity for a Histotechnician in a brand new laboratory!
Nueterra Pathology Services in Irving, TX is looking for a certified HT
or HTL to run their newly constructed laboratory. Candidate must be ASCP
certified and CLIA certified to perform gross dissection, prior
supervisory experience preferred. The candidate will be responsible for
the following: Creation and maintenance of policies and procedures to
CLIA standards, leading lab through CLIA inspection, maintenance and
quality control for equipment, and routine histology duties. The
position offers a competitive salary, medical/dental insurance, 401K
plan, and vacation/sick leave. Interested applicants should contact
Meredith Hale phone 214-596-2219 or through email at mhale@carisdx.com
 . 

 

 

 

 

Meredith Hale HT (ASCP) 

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mhale@carisls.com 

 

 

 

 

 

 

From RSimoskevitz <@t> osteotech.com  Tue Jul 27 15:26:04 2010
From: RSimoskevitz <@t> osteotech.com (Ricki Simoskevitz)
Date: Tue Jul 27 15:26:10 2010
Subject: [Histonet] Ketamine Supplier
In-Reply-To: <20100727170550.AABE82F381F0@l3inpf3.contentcatcher.com>
References: <20100727170550.AABE82F381F0@l3inpf3.contentcatcher.com>
Message-ID: 

I was wondering if anyone could help me find a supplier for Ketamine.  We usually order ours from Sigma but they are back ordered until mid September.  I have considered trying to order Ketamine/Xylazine solution in place of the powdered forms but I don't know what ratio to get.  We use this for athymic rat surgery administered IP.

Thanks for any help you can give me.

Vendors are welcome to reply.

Ricki Simoskevitz
Research Associate
Osteotech Inc.
rsimoskevitz@osteotech.com
732-542-2800 X6328


CONFIDENTIAL COMMUNICATION:  E-mails from Osteotech generally contain information that is confidential, privileged, and/or protected as work product or by other legal rules, and are for the sole use of the intended recipient.  Any use, copying, or distribution of this e-mail by an unintended recipient is prohibited, and may be a violation of law.  If you believe that you received this e-mail in error, please do not read this e-mail or any attached items, and instead please delete the e-mail and all attachments.

From marktarango <@t> gmail.com  Tue Jul 27 16:58:25 2010
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Tue Jul 27 16:58:31 2010
Subject: [Histonet] Using GEWF solution and IHC staining
In-Reply-To: 
References: 
Message-ID: 

Ethanol/alcohol is what will process the specimen.  If the tissue is fixed
would it really matter that the tissue came into contact with ethanol?

Mark
On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB) <
Randi.Hayes@horizonnb.ca> wrote:

> At a recent conference, our PA learned of using GEWF (glacial acetic acid,
> ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph
> Node retrieval in Colorectal Cancer resections.  Although a good idea, I'm
> wondering how "safe" it is to use when staining for IHC.  Does anyone have
> much experience with this or know of a study (studies) that have been done
> to verify that the ethanol is not destroying antigen sites?  We're a little
> concerned......
>
>
> Randi Hayes, MLT
> Histology Supervisor / Superviseur d'Histologie
> Horizon Health Network / R?seau de sant? Horizon
> (506) 860-2157
> randi.hayes@HorizonNB.ca 
> www.HorizonNB.ca  
>
>
>
>
------- Horizon Health Network Disclaimer -------

This e-mail > communication (including any or all attachments) is intended
only for the > use of the person or entity to which it is addressed and may
contain > confidential and/or privileged material. If you are not the > intended
recipient of this e-mail, any use, review, retransmission, > distribution,
dissemination, copying, printing, or other use of, or > taking of any action in
reliance upon this e-mail, is strictly > prohibited. If you have received this
e-mail in error, please contact the > sender and delete the original and any
copy of this e-mail and any > printout thereof, immediately. Your
co-operation is > appreciated.

Le pr?sent courriel (y compris toute pi?ce jointe) > s'adresse uniquement ?
son destinataire, qu'il soit une personne ou un > organisme, et pourrait
comporter des renseignements privil?gi?s ou > confidentiels. Si vous n'?tes
pas le destinataire du courriel, il est > interdit d'utiliser, de revoir, de
retransmettre, de distribuer, de > diss?miner, de copier ou d'imprimer ce
courriel, d'agir en vous y fiant > ou de vous en servir de toute autre fa?on.
Si vous avez re?u le pr?sent > courriel par erreur, pri?re de communiquer
avec l'exp?diteur et > d'?liminer l'original du courriel, ainsi que toute copie
?lectronique ou > imprim?e de celui-ci, imm?diatement. Nous sommes
reconnaissants de votre > collaboration.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From jerrysedgewick <@t> gmail.com  Tue Jul 27 18:06:14 2010
From: jerrysedgewick <@t> gmail.com (Jerry (Gerald) Sedgewick)
Date: Tue Jul 27 18:06:25 2010
Subject: [Histonet] Re: how to isolate scabs
In-Reply-To: <6F33D8418806044682A391273399860F049FD112@s-irv-ex301.PathologyPartners.intranet>
References: <6F33D8418806044682A391273399860F049FD112@s-irv-ex301.PathologyPartners.intranet>
Message-ID: <4C4F6666.7020408@gmail.com>


   Hello all,
   I  have  received embolic material in test tubes in 10% formalin.  The
   tubes  had  been  sitting around for about 2 years.  To quantitate the
   number  of embolic particles, after removing particles at less than 40
   microns,  the  solution was poured into a petri dish and then scanned.
   However,  in  about 1/3rd of the test tubes, a hard disk of what looks
   like  red  blood  cells formed on the bottom when the RBCs should have
   been  suspended  in solution.  These hard discs (scabs) were broken up
   and also included in the petri dish scans.
   The  problem  is  that the scabs should not be measured with the other
   particles because these weren't measured in a previous study: the RBCs
   in a previous study did not coagulate.
   So now I have to figure out which of the broken up scabs are scabs and
   which  could be other embolic material.  In the past, I irradiated the
   RBCs  with  green  light  and  collected through a red filter and this
   revealed  RBC laden particles.  However, these aren't autofluorescing,
   possibly  because  they  have been in solution so long (or because the
   scabs are a mix of embolic materials).
   Here's  the  million dollar question: has anyone had to identify scabs
   on  a  macro  level,  and  how  was that done?  I know all the embolic
   material  can  be  spun down and sectioned, but the idea is to get the
   quantitative  information  about  the  nature  of the particles BEFORE
   spinning down.
   Thanks!
   Jerry

-- 
Jerry (Gerald) Sedgewick
Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output." 

Sedgewick Initiatives
965 Cromwell Avenue
Saint Paul, MN  55114
651-788-2261
[1]jerrysedgewick@gmail.com
[2]http://www.quickphotoshop.com
[3]http://www.rawlight.com
[4]http://www.jerrysedgewick.com

References

   1. mailto:jerrysedgewick@gmail.com
   2. http://www.quickphotoshop.com/
   3. http://www.rawlight.com/
   4. http://www.jerrysedgewick.com/
From AnthonyH <@t> chw.edu.au  Tue Jul 27 18:57:49 2010
From: AnthonyH <@t> chw.edu.au (Tony Henwood)
Date: Tue Jul 27 18:58:13 2010
Subject: [Histonet] Brain Problems
In-Reply-To: <42DFE1A029181B4B8CCBA7261B52D765013A77D4@suffieldex01.suffield.drdc-rddc.gc.ca>
Message-ID: 

The fixation time is too short.

Fix as long as you can (how about 2-3 days at least for brain?)

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poole, Kimberly
Sent: Wednesday, 28 July 2010 12:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Brain Problems


Hi there,

 

I am currently using rat brain for histology but something is not working. I follow the protocol below for tissue processing.

 

Station

Solution

Routine (mins)

 

 

 

1

Formalin

10

2

Formalin

10

3

70% Ethanol

60

4

80% Ethanol

240

5

96% Ethanol

240

6

100% Ethanol

240

7

Xylene

120

8

Xylene

120

9

Xylene

120

10

Paraffin Wax

120

11

Paraffin Wax

180

12

Paraffin Wax

180

TOTAL TIME

 

27 hours 20 minutes

 

 

	

 

I embedded my brains and then cut them afterwards. When the brain section hits the water, it looks like a blob of fat on the water. Then it spreads reall, really slowly. If a cut is made to the paraffin the brain bursts open and it falls apart. Am I doing something wrong in the processing stage? Is the tissue dehydrated too much. Any feedback would be greatly appreciated.

Kimberly Poole B.Sc

Casualty Management Section | Section de la gestion des bless?s

Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield 

Medicine Hat, AB, Canada T1A 8K6

kimberly.poole@drdc-rddc.gc.ca  

Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 

Government of Canada | Gouvernement du Canada

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From thalita <@t> cipax.com.br  Wed Jul 28 05:28:01 2010
From: thalita <@t> cipax.com.br (Thalita)
Date: Wed Jul 28 05:27:03 2010
Subject: [Histonet] RES: Histonet Digest, Vol 80, Issue 32
In-Reply-To: <20100727170726.DF33238004F6@bastion06.email.alog.com.br>
References: <20100727170726.DF33238004F6@bastion06.email.alog.com.br>
Message-ID: <002701cb2e3f$8eb84750$ac28d5f0$@com.br>

Vanusa,

N?o tenho permiss?o para mexer no Igecor e n?o entendi o que deveria ser
arrumado.

Att,


Thalita Ferraz
Produ??o
+55 12 3203-0612 (DIRETO)
+55 12 3203-0633 (SAC)
www.cipax.com.br
thalita@cipax.com.br


-----Mensagem original-----
De: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Em nome de
histonet-request@lists.utsouthwestern.edu
Enviada em: ter?a-feira, 27 de julho de 2010 14:07
Para: histonet@lists.utsouthwestern.edu
Assunto: Histonet Digest, Vol 80, Issue 32

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Today's Topics:

   1. Re:  [Sectioning]  Brain Tissue (Nancy Herman)
   2. Re: Powdered reagent expiration dates (Pat Laurie)
   3. Tissue processing protocol for Hamster tissues (abijag )


----------------------------------------------------------------------

Message: 1
Date: Tue, 27 Jul 2010 11:25:54 -0400
From: "Nancy Herman" 
Subject: [Histonet] Re:  [Sectioning]  Brain Tissue
To: ,	"Margaret Sherwood"
	
Message-ID: <4C4EA622.6DEB.00DD.0@inspection.gc.ca>
Content-Type: text/plain; charset=US-ASCII

We keep our water bath at 40-41 C.  Sometimes we add some 70% ethanol to the
water bath and this helps to get the wrinkles out (especially for cerebellum
and mice brain).  Most of our tissue is bovine brain but we deal with
multiple other species including mice.  We use McCormick Paraplast Plus
embedding Medium from Fisher.

Nancy Herman
Canadian Food Inspection Agency - Lethbridge Laboratory

>>> "Sherwood, Margaret "  2010/07/27 9:14 am >>>
To All:
 
We have been experiencing problems sectioning mouse brain.  The sectioning
is
fine, but we have problems in the water bath.  At 45 degrees C (peel-a-way
paraffin-Polysciences), the sections break apart but we don't get wrinkles.
With paraplast extra, we use 36 degrees C, but the sections are wrinkled and
disintegrate.   
 
I would appreciate hearing from listers as to what you do:  what temperature
is
your water bath, what embedding media do you use, etc?  Any help would be
appreciated!
 
Thanks!
Peggy
 
Peggy Sherwood
Lab Associate, Photopathology 
Wellman Center for Photomedicine (EDW 214)
Massachusetts General Hospital
55 Fruit Street
Boston, Massachusetts 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org 
 


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------------------------------

Message: 2
Date: Tue, 27 Jul 2010 09:00:20 -0700
From: Pat Laurie 
Subject: Re: [Histonet] Powdered reagent expiration dates
To: lpwenk@sbcglobal.net
Cc: Histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

Everyone,

Thanks for your help, based off your explanations, statements and citations,
we will attempt to protest this and have it expunged.



On Mon, Jul 26, 2010 at 4:41 PM, Lee & Peggy Wenk
wrote:

> See if you can get the following article. Biotech Histochem is published
by
> the Biological Stain Commission. http://www.biologicalstaincommission.org/
>
> Biotech Histochem. 2009 Feb;84(1):11-5.
>
> Stain and dye stability over a 30-year period: a comparison of certified
> dye
> powders by the Biological Stain Commission.
> Penney DP, Frank M, Fagan C, Willis C.
>
> Biological Stain Commission, Department of Pathology & Laboratory
Medicine,
> University of Rochester Medical Center, Rochester, New York 14642-0001,
> USA.
> David_Penney@urmc.rochester.edu
>
> Abstract
> The Biological Stain Commission (BSC) Assay Laboratory has received
> numerous
> inquiries during the past several years regarding the long-term stability
> of
> stain and dye powders, particularly since packaging requirements call for
> expiration dates on reagents. We have conducted a study to examine the
> long-term stability of selected dye powders. We used the standard
> procedures
> of the BSC for testing biological stains for certification to give an
> indication of the long-term chemical stability as well as staining
> performance of the dye powders. An earlier study by Emmel and Stotz
> examined
> the stability of various dye powders after a five-year storage period. The
> present study is a follow-up project covering the same dyes after storage
> for 30 years. The dye samples chosen for the study are the same samples
> used
> in the five-year storage period study and give comparative results for all
> three time periods. The results of this study affirm the generally held
> speculation that dye powders are stable for many years and thus have a
> substantial shelf-life.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> Beaumont Hospital
> Royal Oak, MI 48073
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie
> Sent: Monday, July 26, 2010 1:27 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Powdered reagent expiration dates
>
>  We were inspected by CAP on friday and we were cited for
>
>
> ANP.21366  *Are reagents and solutions properly labeled, as applicable and
> appropriate, with the following elements?*
>
> * *
>
> 1.      *Content and quantity, concentration or titer*
>
> 2.      *Storage requirements*
>
> 3.      *Date prepared or reconstituted by laboratory*
>
> 4.      *Expiration date*
>
> Specifically that our staining powders didn't have an expiration date
> printed on the bottle. All of our reconsituted reagents which are in use
> were dated with an expiration date properly though. I have always assumed,
> perhaphs incorrectly, that powdered stains never expire.  We have powders
> like Luxol Echt Blau, etc. that were purchased and opened over 40
> years ago.    If so, then these powdered reagents have gone through CAP
> inspections since the beginning and this inspector was the first one to
> find
> this problem.  Is this one that we might protest?
>
> --
> Patrick Laurie HT(ASCP)QIHC
> CellNetix Pathology & Laboratories
> 1124 Columbia Street, Suite 200
> Seattle, WA 98104
> PH: 206-215-5949
> plaurie@cellnetix.com
>  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie@cellnetix.com


------------------------------

Message: 3
Date: 27 Jul 2010 15:30:26 -0000
From: "abijag " 
Subject: [Histonet] Tissue processing protocol for Hamster tissues
To: 
Message-ID: <20100727153026.31966.qmail@f6mail-144-201.rediffmail.com>
Content-Type: text/plain; charset="UTF-8"

Dear Histonetters,

We were asked to process Hamster tissue(Large intestine) for one of our
investigators. I would like to know whether any of our users process hamster
tissues in their labs, if so kindly provide me a protocol for the same. Our
tissue processor is Sakura VIP6.



Thanks for all



Abi jagannath

------------------------------

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End of Histonet Digest, Vol 80, Issue 32
****************************************


From Masterson_John <@t> allergan.com  Wed Jul 28 08:59:07 2010
From: Masterson_John <@t> allergan.com (Masterson_John)
Date: Wed Jul 28 09:00:57 2010
Subject: [Histonet] Histology Tech Position In Irvine CA.
In-Reply-To: <2040770931786624083902483686662@psmtp.com>
References: <2040770931786624083902483686662@psmtp.com>
Message-ID: 


Allergan Inc. in Irvine California is looking to fill a position in our DSE Pathology Dept. If interested please see details on the Allergan web site.

This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation.

From jstaruk <@t> masshistology.com Wed Jul 28 09:26:04 2010 From: jstaruk <@t> masshistology.com (jstaruk) Date: Wed Jul 28 09:26:06 2010 Subject: [Histonet] ? on Gulf oil spill In-Reply-To: Message-ID: <42EAEA73F8E04AC7BDF054439EC517E7@JimPC> Hi all, I have a customer who wants to study the amounts of oil accumulation in the gills of fish from the Gulf of Mexico. Does anyone have any suggestions as to what techniques might stain crude oil? Thanks Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com From rjr6 <@t> psu.edu Wed Jul 28 10:25:38 2010 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Wed Jul 28 10:26:01 2010 Subject: [Histonet] ? on Gulf oil spill In-Reply-To: <42EAEA73F8E04AC7BDF054439EC517E7@JimPC> References: <42EAEA73F8E04AC7BDF054439EC517E7@JimPC> Message-ID: I did an Oil Red O on beaver tissues from a place there was a diesel spill and it worked out pretty well. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, July 28, 2010 10:26 AM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] ? on Gulf oil spill Hi all, I have a customer who wants to study the amounts of oil accumulation in the gills of fish from the Gulf of Mexico. Does anyone have any suggestions as to what techniques might stain crude oil? Thanks Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Wed Jul 28 11:11:13 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Jul 28 11:11:24 2010 Subject: [Histonet] Sakura VIP6 Message-ID: <000801cb2e6f$80cbab70$82630250$@com> Help! I am having a problem with me VIP6 tissue processor. About every 4 to 6 weeks the processor has a error code that position 2 which is 80% has not pumped in fully in the allotted time. Sakura answers to this problem is either I need to use their formalin or the alcohol in the second station is too high a concentration. I have never had this problem before in any processor I have used. I still use the Shandon excelsiour tissue processor and use the same protocol in both machines with no problems in the Shandon. Sakura thinks it is a build-up of precipitating salts that is causing the problem but there was no sign of this when the service tech checked the rotary valve. After every process we not only flush with ETOH and xylene but also run a warm water flush from the first station which is formalin. I use a different bottle for the warm water but do use the same valve from the formalin bottle. When we have completed 7 runs we run the 3 warm water flush Sakura recommends. Even the service tech remarked that I was doing more than they recommend. I am at a loss as to what it could be. My only conclusion is that maybe the valve in station 2 intermittently collapse or sticks for some reason. I wanted to see if anyone either is having the same problem or if someone has a thought of what the problem could be. I did purchase a refurbished machine. I would appreciate any thoughts, ideas, or even guesses that anyone has. Thanks in advance for any help. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com From Nacaela.Johnson <@t> USONCOLOGY.COM Wed Jul 28 12:11:42 2010 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Wed Jul 28 12:11:47 2010 Subject: [Histonet] Gordon and Sweets retic stain Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D232@txhous1eb012.uson.usoncology.int> Hello! I am having a problem with my tissue washing off of the slides during the retic stain. The majority of the tissue is falling off during the Working silver solution step because of the alkalinity of the solution. The tissue is bone marrow and I use Halt in my water bath. My thoughts are to add a mild acid to the working solution to bring the pH down, but I am not sure of what the effects are on the solution. I do not want the silver to not impregnate. Has anyone had this problem before and found a solution? Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From jclark <@t> pcnm.com Wed Jul 28 13:34:49 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Jul 28 13:34:53 2010 Subject: [Histonet] RE: Histonet Digest, Vol 80, Issue 33 Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C012F797B@mail.pcnm.com> We use a similar product called Dissect Aid, from Decal Corp. IHC staining has never been an issue; we never run IHC on the lymph nodes from colon cancer cases. We usually do the IHC on the tumor itself which is fixed in formalin. This kind of product is very useful in helping to find all those pesky little nodes that like to hide in the fat by turning them white. Cancer protocols require that at least 13 nodes be submitted. As histo supervisor, I also do the grossing in my facility, so I understand why your PA is interested in this kind of product (it can be difficult to come up with this magic number of 13 without it). However, if you do run IHC on the nodes from these cases, you would indeed have to revalidate your markers with this as your primary fixative or as a post fixative. Joanne Clark, HT, MLT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM ----------------------------- Message: 2 Date: Tue, 27 Jul 2010 15:03:33 -0300 From: "Hayes, Randi (HorizonNB)" Subject: [Histonet] Using GEWF solution and IHC staining To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" At a recent conference, our PA learned of using GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph Node retrieval in Colorectal Cancer resections. Although a good idea, I'm wondering how "safe" it is to use when staining for IHC. Does anyone have much experience with this or know of a study (studies) that have been done to verify that the ethanol is not destroying antigen sites? We're a little concerned...... Randi Hayes, MLT Histology Supervisor / Superviseur d'Histologie Horizon Health Network / R?seau de sant? Horizon (506) 860-2157 randi.hayes@HorizonNB.ca www.HorizonNB.ca From cpyse <@t> x-celllab.com Wed Jul 28 13:40:21 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Jul 28 13:40:31 2010 Subject: [Histonet] Sakura VIP6 update Message-ID: <002101cb2e84$55ced450$016c7cf0$@com> Thanks everyone for your responses. Sakura has scheduled an upgrade of my rotary and gate valve. I hope this is the answer I need. Cindy From Bill.Tench <@t> pph.org Wed Jul 28 14:12:30 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Jul 28 14:12:36 2010 Subject: [Histonet] dissection aide Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A54A9@MAIL1.pph.local> I don't see much advantage of this over plain old Carnoy's fixative, other than missing the wiff of choroform. Ethanol should not be a problem with IHC, but as you said, revalidation is required. The magic number of nodes is 12. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- From sbreeden <@t> nmda.nmsu.edu Wed Jul 28 15:41:04 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jul 28 15:41:11 2010 Subject: [Histonet] Refrigerate formalin? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47292@nmdamailsvr.nmda.ad.nmsu.edu> We're getting ready to move into our new building (YAHOO!) in early September - finally. In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C). Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists. Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 From rjbuesa <@t> yahoo.com Wed Jul 28 15:49:52 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 28 15:49:56 2010 Subject: [Histonet] Refrigerate formalin? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47292@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <304094.35306.qm@web65715.mail.ac4.yahoo.com> There is nothing against cooling formalin fixed tissues, but my question is WHY? I see it as a waste of refrigerated space. On the other hand also, IF by any change there is a formalin spill inside the walk-in?cooler room, that will be a royal mess to decontaminate. Ren? J. --- On Wed, 7/28/10, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Refrigerate formalin? To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 28, 2010, 4:41 PM We're getting ready to move into our new building (YAHOO!) in early September - finally.? In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C).???Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time?? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists.? Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM? 87108 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Wed Jul 28 15:50:28 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Jul 28 15:50:33 2010 Subject: [Histonet] REFRIG FORMALIN Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A54AA@MAIL1.pph.local> You must have a heck of a lot of space in the frig. I am envious. I don't think refrigerating your specimens would create any problems. Everyone i know stores them in some inconvenient corner of a morgue at room temperature. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- From darkdaym <@t> comcast.net Wed Jul 28 16:24:37 2010 From: darkdaym <@t> comcast.net (Mark Ray) Date: Wed Jul 28 16:24:46 2010 Subject: [Histonet] Refrigerate formalin? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47292@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E47292@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4C50A015.40005@comcast.net> It is usually recommended that 10% Formalin Fixative be stored at temperatures above about 10C to minimize polymerization of Formaldehyde. You might confirm this with the manufacturer of your Formalin Fixative. The label may also say something about this. Breeden, Sara wrote: > We're getting ready to move into our new building (YAHOO!) in early > September - finally. In planning where we will store our cases for the > required 6-week retention time, I have proposed that the tissues (in 10% > NBF) be shelved in the walk-in cooler (4 degrees C). Is there any > reason tissues-in-formalin could NOT be stored refrigerated for that > length of time? It is extremely rare that we are required to pull and > recut wet tissue, but that possibility always exists. Thanks, everyone. > > > > Sally Breeden, HT(ASCP) > > Veterinary Diagnostic Services > > New Mexico Department of Agriculture > > 700 Camino de Salud NE > > Albuquerque, NM 87108 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From leswes <@t> shaw.ca Wed Jul 28 17:54:18 2010 From: leswes <@t> shaw.ca (Lesley Weston) Date: Wed Jul 28 17:54:24 2010 Subject: [Histonet] ? on Gulf oil spill In-Reply-To: <42EAEA73F8E04AC7BDF054439EC517E7@JimPC> References: <42EAEA73F8E04AC7BDF054439EC517E7@JimPC> Message-ID: <378EA25A-D24E-4961-9684-6370B09BC5A3@shaw.ca> Oil Red O might work, since it stains lipids. Lesley Weston. On 2010-07-28, at 7:26 AM, jstaruk wrote: > Hi all, > > I have a customer who wants to study the amounts of oil accumulation in the > gills of fish from the Gulf of Mexico. Does anyone have any suggestions as > to what techniques might stain crude oil? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vhlwong <@t> yahoo.com Wed Jul 28 23:05:45 2010 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Wed Jul 28 23:05:49 2010 Subject: [Histonet] PECAM-1 (sc-1506R) staining Message-ID: <983283.48384.qm@web52701.mail.re2.yahoo.com> Dear all, ? Thank you for all the professional suggestion of the previous IHC staining and finally I got some positive results. ? Then comes problem on the PECAM-1 IHC.? I am using the Santa Cruz sc-1506R (it is a anti-RAT antibody from RABBIT).? I stain decalcified rat spine paraffin sections, with following steps: ? antigen retrieval (~95C MW, DAKO AR solution pH6 for 20 minutes); DAKO's Dual Enzyme block for 10 minutes; 3% normal goat serum for 20 minutes; 1:50 primary antibody at 4C for overnight; DAKO's Envision anti-rabbit?kit for 30 minutes; DAKO's DAB solution to develop ? I used rat liver and kidney?as positive controls.? I got background staining on hepatocytes but none staining on vessels.? On kidney section, nucleus and lining within?tubules and basement membrane of glomerulus were also stained but not that bad as liver.? I cannot say it was totally non-specific as some nucleus were not stained. ? I think PECAM-1 IHC needs more specific conditons.? Could any histoneters have any suggestion? ? Many thanks in advance. ? Victor From Nacaela.Johnson <@t> USONCOLOGY.COM Thu Jul 29 07:49:02 2010 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Thu Jul 29 07:51:15 2010 Subject: [Histonet] Refrigerate formalin? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47292@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E47292@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D237@txhous1eb012.uson.usoncology.int> I had a retic kit that was put in the refrigerator by one of my collegues. The formalin was stated to be stored at room temp, but was left in the box. The cold temperature broke the formalin down and it no longer worked to reduce the silver. My suggestion would be to keep them at room temperature to be on the safe side. Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, July 28, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerate formalin? We're getting ready to move into our new building (YAHOO!) in early September - finally. In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C). Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists. Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From MW <@t> PersonifySearch.com Thu Jul 29 08:15:03 2010 From: MW <@t> PersonifySearch.com (Matthew Ward) Date: Thu Jul 29 08:15:08 2010 Subject: [Histonet] NEW Opportunity with a World Leader in IHC in New England! Message-ID: <010f01cb2f20$0eb960c0$2c2c2240$@com> Good Morning All, Our team at Personify is currently partnered with a World Leader in IHC that is currently looking for a Field Support Specialist in the New England area. This is the perfect opportunity for a histotech to move off the bench and get into the field on the manufacturers side! The position offers the following: Base Salary! Bonus! Car Allowance and Paid Expenses! Outstanding Benefits! Opportunity for advancement! Please contact me immediately to learn more! Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From mcauliff <@t> umdnj.edu Thu Jul 29 08:47:59 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jul 29 08:45:21 2010 Subject: [Histonet] Refrigerate formalin? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47292@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E47292@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4C51868F.90302@umdnj.edu> I agree with Rene and Mark. Geoff Breeden, Sara wrote: > We're getting ready to move into our new building (YAHOO!) in early > September - finally. In planning where we will store our cases for the > required 6-week retention time, I have proposed that the tissues (in 10% > NBF) be shelved in the walk-in cooler (4 degrees C). Is there any > reason tissues-in-formalin could NOT be stored refrigerated for that > length of time? It is extremely rare that we are required to pull and > recut wet tissue, but that possibility always exists. Thanks, everyone. > > > > Sally Breeden, HT(ASCP) > > Veterinary Diagnostic Services > > New Mexico Department of Agriculture > > 700 Camino de Salud NE > > Albuquerque, NM 87108 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From sarah_taba <@t> yahoo.com Thu Jul 29 09:04:29 2010 From: sarah_taba <@t> yahoo.com (sarah Tabatabaei) Date: Thu Jul 29 09:04:33 2010 Subject: [Histonet] FAST interpretation Message-ID: <395564.60128.qm@web45707.mail.sp1.yahoo.com> Hi, I'm using FAST profile (Fast green, Alcian Blue, Safranin-O, and Tartrazine) to stain my human Intervertebral Discs. how can I figure out which color resembles which type of tissue? Regards -Sarah From sbreeden <@t> nmda.nmsu.edu Thu Jul 29 10:04:46 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Jul 29 10:04:52 2010 Subject: [Histonet] Cool Formalin Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47294@nmdamailsvr.nmda.ad.nmsu.edu> Thanks to everyone that replied to my question about refrigerating 10% NBF for up to 6 weeks. I'll fold your information into the decision. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 From dixonm <@t> ufl.edu Thu Jul 29 10:34:43 2010 From: dixonm <@t> ufl.edu (Dixon,Maryann) Date: Thu Jul 29 10:34:50 2010 Subject: [Histonet] plastics Message-ID: Hi histoland, Thinking of getting into plastics and need to know information, pros/cons about them. I've never worked with anything else except paraffin. I will be embedding mostly osteosarcomas. Can someone please give me a call or email me. All suggestions are very appreciated. Thank you. MaryAnn Dixon BS, HT (ASCP)cm Biological Scientist Surgical Oncology UF College of Veterinary Medicine Phone (352) 294-4516 Email: dixonm@ufl.edu From dixonm <@t> ufl.edu Thu Jul 29 11:54:51 2010 From: dixonm <@t> ufl.edu (Dixon,Maryann) Date: Thu Jul 29 11:54:56 2010 Subject: [Histonet] knife holder Message-ID: Greetings histonetters!!! Does anyone out there have a paraffin knife holder (knife holder B) for a Leica SM2500 polycut microtome that you might like to part with. I am in need of finding one. Thank you. MaryAnn Dixon BS, HT (ASCP)cm Biological Scientist Surgical Oncology UF College of Veterinary Medicine Phone (352) 294-4516 Email: dixonm@ufl.edu From ross <@t> premierlab.com Thu Jul 29 11:56:56 2010 From: ross <@t> premierlab.com (Ross Benik) Date: Thu Jul 29 11:56:21 2010 Subject: [Histonet] p53 IHC staining in mouse Message-ID: Hi everyone, I am trying to accomplish IHC staining for a rabbit anti p53 antibody in mouse tissue however all I am getting is background and IgG staining. I run my human positive control tissue along with the same protocol parameters and the staining is perfect. I have tried two abcam antibodies (ab32049 and ab4060) and both are not working correctly on mouse tissue. Does anyone know of a p53 antibody that is known to work in mouse tissue? Thanks, Ross From rsrichmond <@t> gmail.com Thu Jul 29 12:39:57 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Jul 29 12:40:02 2010 Subject: [Histonet] Re: Using GEWF solution and IHC staining Message-ID: GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde) solution, Davidson's fixative (3 parts water, 3 parts alcohol, 2 parts 37% formaldehyde, 1 part glacial acetic acid), and various proprietary products (Dissect-Aid, O-Fix, and others) are all used to fix fatty tissue in order to recover as many lymph nodes as possible. The unfixed fatty tissue is separated from the rest of the specimen and fixed overnight in the "disclosing fixative". A good many pathologists, including me, like these fixatives for colon cancers. I've also used it for radical neck dissection specimens. It's not necessary for axillary dissection specimens for breast cancer, since the lymph nodes are big and easy to find. Bob Richmond Samurai Pathologist Knoxville TN From pvlies <@t> yahoo.com Thu Jul 29 12:42:03 2010 From: pvlies <@t> yahoo.com (pvlies@yahoo.com) Date: Thu Jul 29 12:42:13 2010 Subject: [Histonet] : Gulf oil ? Histonet Digest, Vol 80, Issue 34 Message-ID: <1213903528-1280425325-cardhu_decombobulator_blackberry.rim.net-554163664-@bda2466.bisx.prod.on.blackberry> Yes, ORO stains lipids but I don't think it would work for crude. Would you look for artifact and tissue reaction? Pam Vlies, HT-ASCP Waukegan IL Sent on the Sprint? Now Network from my BlackBerry? -----Original Message----- From: histonet-request@lists.utsouthwestern.edu Sender: histonet-bounces@lists.utsouthwestern.edu To: Reply-To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 34 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Gordon and Sweets retic stain (Johnson, Nacaela) 2. RE: Histonet Digest, Vol 80, Issue 33 (Joanne Clark) 3. Sakura VIP6 update (Cynthia Pyse) 4. dissection aide (Tench, Bill) 5. Refrigerate formalin? (Breeden, Sara) 6. Re: Refrigerate formalin? (Rene J Buesa) 7. REFRIG FORMALIN (Tench, Bill) 8. Re: Refrigerate formalin? (Mark Ray) 9. Re: ? on Gulf oil spill (Lesley Weston) 10. PECAM-1 (sc-1506R) staining (Victor Wong) 11. RE: Refrigerate formalin? (Johnson, Nacaela) 12. NEW Opportunity with a World Leader in IHC in New England! (Matthew Ward) 13. Re: Refrigerate formalin? (Geoff McAuliffe) 14. FAST interpretation (sarah Tabatabaei) 15. Cool Formalin (Breeden, Sara) 16. plastics (Dixon,Maryann) 17. knife holder (Dixon,Maryann) 18. p53 IHC staining in mouse (Ross Benik) ---------------------------------------------------------------------- Message: 1 Date: Wed, 28 Jul 2010 12:11:42 -0500 From: "Johnson, Nacaela" Subject: [Histonet] Gordon and Sweets retic stain To: Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D232@txhous1eb012.uson.usoncology.int> Content-Type: text/plain; charset="us-ascii" Hello! I am having a problem with my tissue washing off of the slides during the retic stain. The majority of the tissue is falling off during the Working silver solution step because of the alkalinity of the solution. The tissue is bone marrow and I use Halt in my water bath. My thoughts are to add a mild acid to the working solution to bring the pH down, but I am not sure of what the effects are on the solution. I do not want the silver to not impregnate. Has anyone had this problem before and found a solution? Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. ------------------------------ Message: 2 Date: Wed, 28 Jul 2010 12:34:49 -0600 From: "Joanne Clark" Subject: [Histonet] RE: Histonet Digest, Vol 80, Issue 33 To: Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C012F797B@mail.pcnm.com> Content-Type: text/plain; charset="iso-8859-1" We use a similar product called Dissect Aid, from Decal Corp. IHC staining has never been an issue; we never run IHC on the lymph nodes from colon cancer cases. We usually do the IHC on the tumor itself which is fixed in formalin. This kind of product is very useful in helping to find all those pesky little nodes that like to hide in the fat by turning them white. Cancer protocols require that at least 13 nodes be submitted. As histo supervisor, I also do the grossing in my facility, so I understand why your PA is interested in this kind of product (it can be difficult to come up with this magic number of 13 without it). However, if you do run IHC on the nodes from these cases, you would indeed have to revalidate your markers with this as your primary fixative or as a post fixative. Joanne Clark, HT, MLT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM ----------------------------- Message: 2 Date: Tue, 27 Jul 2010 15:03:33 -0300 From: "Hayes, Randi (HorizonNB)" Subject: [Histonet] Using GEWF solution and IHC staining To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" At a recent conference, our PA learned of using GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph Node retrieval in Colorectal Cancer resections. Although a good idea, I'm wondering how "safe" it is to use when staining for IHC. Does anyone have much experience with this or know of a study (studies) that have been done to verify that the ethanol is not destroying antigen sites? We're a little concerned...... Randi Hayes, MLT Histology Supervisor / Superviseur d'Histologie Horizon Health Network / R?seau de sant? Horizon (506) 860-2157 randi.hayes@HorizonNB.ca www.HorizonNB.ca ------------------------------ Message: 3 Date: Wed, 28 Jul 2010 14:40:21 -0400 From: "Cynthia Pyse" Subject: [Histonet] Sakura VIP6 update To: Message-ID: <002101cb2e84$55ced450$016c7cf0$@com> Content-Type: text/plain; charset="us-ascii" Thanks everyone for your responses. Sakura has scheduled an upgrade of my rotary and gate valve. I hope this is the answer I need. Cindy ------------------------------ Message: 4 Date: Wed, 28 Jul 2010 12:12:30 -0700 From: "Tench, Bill" Subject: [Histonet] dissection aide To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A54A9@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii I don't see much advantage of this over plain old Carnoy's fixative, other than missing the wiff of choroform. Ethanol should not be a problem with IHC, but as you said, revalidation is required. The magic number of nodes is 12. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- ------------------------------ Message: 5 Date: Wed, 28 Jul 2010 14:41:04 -0600 From: "Breeden, Sara" Subject: [Histonet] Refrigerate formalin? To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47292@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" We're getting ready to move into our new building (YAHOO!) in early September - finally. In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C). Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists. Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ------------------------------ Message: 6 Date: Wed, 28 Jul 2010 13:49:52 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Refrigerate formalin? To: histonet@lists.utsouthwestern.edu, SaraBreeden Message-ID: <304094.35306.qm@web65715.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 There is nothing against cooling formalin fixed tissues, but my question is WHY? I see it as a waste of refrigerated space. On the other hand also, IF by any change there is a formalin spill inside the walk-in?cooler room, that will be a royal mess to decontaminate. Ren? J. --- On Wed, 7/28/10, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Refrigerate formalin? To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 28, 2010, 4:41 PM We're getting ready to move into our new building (YAHOO!) in early September - finally.? In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C).???Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time?? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists.? Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM? 87108 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 28 Jul 2010 13:50:28 -0700 From: "Tench, Bill" Subject: [Histonet] REFRIG FORMALIN To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A54AA@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii You must have a heck of a lot of space in the frig. I am envious. I don't think refrigerating your specimens would create any problems. Everyone i know stores them in some inconvenient corner of a morgue at room temperature. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- ------------------------------ Message: 8 Date: Wed, 28 Jul 2010 16:24:37 -0500 From: Mark Ray Subject: Re: [Histonet] Refrigerate formalin? To: "Breeden, Sara" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4C50A015.40005@comcast.net> Content-Type: text/plain; charset=ISO-8859-1; format=flowed It is usually recommended that 10% Formalin Fixative be stored at temperatures above about 10C to minimize polymerization of Formaldehyde. You might confirm this with the manufacturer of your Formalin Fixative. The label may also say something about this. Breeden, Sara wrote: > We're getting ready to move into our new building (YAHOO!) in early > September - finally. In planning where we will store our cases for the > required 6-week retention time, I have proposed that the tissues (in 10% > NBF) be shelved in the walk-in cooler (4 degrees C). Is there any > reason tissues-in-formalin could NOT be stored refrigerated for that > length of time? It is extremely rare that we are required to pull and > recut wet tissue, but that possibility always exists. Thanks, everyone. > > > > Sally Breeden, HT(ASCP) > > Veterinary Diagnostic Services > > New Mexico Department of Agriculture > > 700 Camino de Salud NE > > Albuquerque, NM 87108 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 9 Date: Wed, 28 Jul 2010 15:54:18 -0700 From: Lesley Weston Subject: Re: [Histonet] ? on Gulf oil spill To: jstaruk Cc: histonet@lists.utsouthwestern.edu Message-ID: <378EA25A-D24E-4961-9684-6370B09BC5A3@shaw.ca> Content-Type: text/plain; charset=us-ascii Oil Red O might work, since it stains lipids. Lesley Weston. On 2010-07-28, at 7:26 AM, jstaruk wrote: > Hi all, > > I have a customer who wants to study the amounts of oil accumulation in the > gills of fish from the Gulf of Mexico. Does anyone have any suggestions as > to what techniques might stain crude oil? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 28 Jul 2010 21:05:45 -0700 (PDT) From: Victor Wong Subject: [Histonet] PECAM-1 (sc-1506R) staining To: histonet@lists.utsouthwestern.edu Message-ID: <983283.48384.qm@web52701.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear all, ? Thank you for all the professional suggestion of the previous IHC staining and finally I got some positive results. ? Then comes problem on the PECAM-1 IHC.? I am using the Santa Cruz sc-1506R (it is a anti-RAT antibody from RABBIT).? I stain decalcified rat spine paraffin sections, with following steps: ? antigen retrieval (~95C MW, DAKO AR solution pH6 for 20 minutes); DAKO's Dual Enzyme block for 10 minutes; 3% normal goat serum for 20 minutes; 1:50 primary antibody at 4C for overnight; DAKO's Envision anti-rabbit?kit for 30 minutes; DAKO's DAB solution to develop ? I used rat liver and kidney?as positive controls.? I got background staining on hepatocytes but none staining on vessels.? On kidney section, nucleus and lining within?tubules and basement membrane of glomerulus were also stained but not that bad as liver.? I cannot say it was totally non-specific as some nucleus were not stained. ? I think PECAM-1 IHC needs more specific conditons.? Could any histoneters have any suggestion? ? Many thanks in advance. ? Victor ------------------------------ Message: 11 Date: Thu, 29 Jul 2010 07:49:02 -0500 From: "Johnson, Nacaela" Subject: RE: [Histonet] Refrigerate formalin? To: "Breeden, Sara" , Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D237@txhous1eb012.uson.usoncology.int> Content-Type: text/plain; charset="us-ascii" I had a retic kit that was put in the refrigerator by one of my collegues. The formalin was stated to be stored at room temp, but was left in the box. The cold temperature broke the formalin down and it no longer worked to reduce the silver. My suggestion would be to keep them at room temperature to be on the safe side. Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, July 28, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerate formalin? We're getting ready to move into our new building (YAHOO!) in early September - finally. In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C). Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists. Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. ------------------------------ Message: 12 Date: Thu, 29 Jul 2010 09:15:03 -0400 From: "Matthew Ward" Subject: [Histonet] NEW Opportunity with a World Leader in IHC in New England! To: Message-ID: <010f01cb2f20$0eb960c0$2c2c2240$@com> Content-Type: text/plain; charset="us-ascii" Good Morning All, Our team at Personify is currently partnered with a World Leader in IHC that is currently looking for a Field Support Specialist in the New England area. This is the perfect opportunity for a histotech to move off the bench and get into the field on the manufacturers side! The position offers the following: Base Salary! Bonus! Car Allowance and Paid Expenses! Outstanding Benefits! Opportunity for advancement! Please contact me immediately to learn more! Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ------------------------------ Message: 13 Date: Thu, 29 Jul 2010 09:47:59 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] Refrigerate formalin? To: "Breeden, Sara" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4C51868F.90302@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I agree with Rene and Mark. Geoff Breeden, Sara wrote: > We're getting ready to move into our new building (YAHOO!) in early > September - finally. In planning where we will store our cases for the > required 6-week retention time, I have proposed that the tissues (in 10% > NBF) be shelved in the walk-in cooler (4 degrees C). Is there any > reason tissues-in-formalin could NOT be stored refrigerated for that > length of time? It is extremely rare that we are required to pull and > recut wet tissue, but that possibility always exists. Thanks, everyone. > > > > Sally Breeden, HT(ASCP) > > Veterinary Diagnostic Services > > New Mexico Department of Agriculture > > 700 Camino de Salud NE > > Albuquerque, NM 87108 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 14 Date: Thu, 29 Jul 2010 07:04:29 -0700 (PDT) From: sarah Tabatabaei Subject: [Histonet] FAST interpretation To: histonet@lists.utsouthwestern.edu Message-ID: <395564.60128.qm@web45707.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi, I'm using FAST profile (Fast green, Alcian Blue, Safranin-O, and Tartrazine) to stain my human Intervertebral Discs. how can I figure out which color resembles which type of tissue? Regards -Sarah ------------------------------ Message: 15 Date: Thu, 29 Jul 2010 09:04:46 -0600 From: "Breeden, Sara" Subject: [Histonet] Cool Formalin To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47294@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Thanks to everyone that replied to my question about refrigerating 10% NBF for up to 6 weeks. I'll fold your information into the decision. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ------------------------------ Message: 16 Date: Thu, 29 Jul 2010 11:34:43 -0400 From: "Dixon,Maryann" Subject: [Histonet] plastics To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi histoland, Thinking of getting into plastics and need to know information, pros/cons about them. I've never worked with anything else except paraffin. I will be embedding mostly osteosarcomas. Can someone please give me a call or email me. All suggestions are very appreciated. Thank you. MaryAnn Dixon BS, HT (ASCP)cm Biological Scientist Surgical Oncology UF College of Veterinary Medicine Phone (352) 294-4516 Email: dixonm@ufl.edu ------------------------------ Message: 17 Date: Thu, 29 Jul 2010 12:54:51 -0400 From: "Dixon,Maryann" Subject: [Histonet] knife holder To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Greetings histonetters!!! Does anyone out there have a paraffin knife holder (knife holder B) for a Leica SM2500 polycut microtome that you might like to part with. I am in need of finding one. Thank you. MaryAnn Dixon BS, HT (ASCP)cm Biological Scientist Surgical Oncology UF College of Veterinary Medicine Phone (352) 294-4516 Email: dixonm@ufl.edu ------------------------------ Message: 18 Date: Thu, 29 Jul 2010 10:56:56 -0600 From: "Ross Benik" Subject: [Histonet] p53 IHC staining in mouse To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone, I am trying to accomplish IHC staining for a rabbit anti p53 antibody in mouse tissue however all I am getting is background and IgG staining. I run my human positive control tissue along with the same protocol parameters and the staining is perfect. I have tried two abcam antibodies (ab32049 and ab4060) and both are not working correctly on mouse tissue. Does anyone know of a p53 antibody that is known to work in mouse tissue? Thanks, Ross ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 34 **************************************** From Sandra.Harrison3 <@t> va.gov Thu Jul 29 12:50:31 2010 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Thu Jul 29 12:50:37 2010 Subject: [Histonet] labeling of recycled chemicals Message-ID: Dear Histonetters, If you use a recycler for xylene and/or alcohol, how are you labeling the recycled containers? Do you assign a "lot #" to each carboy? Do you keep a log or just label any container filled from a carboy with the "lot #" and the %, in the case of alcohols? Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 From lblazek <@t> digestivespecialists.com Thu Jul 29 13:02:27 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jul 29 13:05:22 2010 Subject: [Histonet] RE: labeling of recycled chemicals In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E390EAF72C68A@IBMB7Exchange.digestivespecialists.com> Sandy, We keep a log that says the alcohol was tested for %, date and tech. The recycled alcohol goes into already labeled containers that notes that it is recycled and the %. The recycled Formula 83 is tested for purity and entered into the log the same way the alcohol is. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Thursday, July 29, 2010 1:51 PM To: histonet@lists.utsouthwestern.edu Cc: Dachel, Susan K. Subject: [Histonet] labeling of recycled chemicals Dear Histonetters, If you use a recycler for xylene and/or alcohol, how are you labeling the recycled containers? Do you assign a "lot #" to each carboy? Do you keep a log or just label any container filled from a carboy with the "lot #" and the %, in the case of alcohols? Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmilne <@t> bccancer.bc.ca Thu Jul 29 13:20:05 2010 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Thu Jul 29 13:20:12 2010 Subject: [Histonet] RE: p53 IHC staining in mouse In-Reply-To: References: Message-ID: <3FEFF18FF4E1914A9AB7D8498591BE86102037FDEF@VEXCCR02.phsabc.ehcnet.ca> I get my mouse p53 Ab from LabVision... http://www.labvision.com/ab.cfm?First=AntiBody&Second=9006 ------------------------------ Message: 18 Date: Thu, 29 Jul 2010 10:56:56 -0600 From: "Ross Benik" Subject: [Histonet] p53 IHC staining in mouse To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone, I am trying to accomplish IHC staining for a rabbit anti p53 antibody in mouse tissue however all I am getting is background and IgG staining. I run my human positive control tissue along with the same protocol parameters and the staining is perfect. I have tried two abcam antibodies (ab32049 and ab4060) and both are not working correctly on mouse tissue. Does anyone know of a p53 antibody that is known to work in mouse tissue? Thanks, Ross ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 34 **************************************** From Jennifer.Bull <@t> northwestpathology.com Thu Jul 29 14:06:53 2010 From: Jennifer.Bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Thu Jul 29 14:07:04 2010 Subject: [Histonet] IHC Technician position available Message-ID: <85760CECEC18444BB95F26D5E88DAEAA22A522106E@hinet2.hinet.org> Northwest Pathology has an opportunity for an Immunohistochemistry Technician, ASCP certified or registry eligible preferred, to join our progressive laboratory. This full-time position requires two to three years of hands on immunohistochemistry experience with QIHC certification or eligibility for certification. Molecular experience is desirable. Responsibilities include a variety of routine and specialized laboratory procedures with an opportunity to learn new testing as we expand our services to the community. NW Pathology, a private laboratory located in Bellingham, WA, which diagnoses over 30,000 specimens a year is located between Seattle, WA and Vancouver, BC, and borders the North Puget Sound including the San Juan Islands. Wilderness areas with glacial rivers and the North Cascades National Park are nearby. We offer a generous comprehensive benefits package, including health and dental insurance, paid time off, 401(k) savings plan and profit sharing. For more information please contact: Jennifer Bull Histology Supervisor - NW Pathology 3614 Meridian St, Suite 101 Bellingham, WA 98225 360-734-2800 x503 jennifer.bull@northwestpathology.com mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- From plucas <@t> biopath.org Thu Jul 29 14:17:54 2010 From: plucas <@t> biopath.org (Paula Lucas) Date: Thu Jul 29 14:13:03 2010 Subject: [Histonet] Sakura Film Removal Message-ID: <577D1AFFCE584BB7A91EAE2214AE990E@biopath.local> Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of H&E slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA From jmcgough <@t> clinlab.com Thu Jul 29 14:18:52 2010 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Thu Jul 29 14:19:00 2010 Subject: [Histonet] Sakura Film Removal In-Reply-To: <577D1AFFCE584BB7A91EAE2214AE990E@biopath.local> Message-ID: We soak the slides in acetone for 5-10 minutes and the film will peel off very easily. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Paula Lucas Sent: Thursday, July 29, 2010 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura Film Removal Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of H&E slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Thu Jul 29 14:19:23 2010 From: annigyg <@t> gmail.com (annigyg@gmail.com) Date: Thu Jul 29 14:19:27 2010 Subject: [Histonet] Sakura Film Removal In-Reply-To: <577D1AFFCE584BB7A91EAE2214AE990E@biopath.local> References: <577D1AFFCE584BB7A91EAE2214AE990E@biopath.local> Message-ID: <1975938924-1280431158-cardhu_decombobulator_blackberry.rim.net-1322361293-@bda242.bisx.produk.on.blackberry> The slips will not come off with xylene Soak in acetone for 5mins The slip swells and falls off Without letting the slide dry or turn milky white, dip into xylene a few times If milkiness appears, dip in clean acetone a few times and back to xylene Recoverslip as and when needed Simple really AnnieinArabia Empower your Business with BlackBerry? and Mobile Solutions from Etisalat -----Original Message----- From: "Paula Lucas" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Thu, 29 Jul 2010 12:17:54 To: Subject: [Histonet] Sakura Film Removal Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of H&E slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sscalise <@t> beaumonthospitals.com Thu Jul 29 14:37:10 2010 From: sscalise <@t> beaumonthospitals.com (Sharon Scalise) Date: Thu Jul 29 14:39:18 2010 Subject: [Histonet] Sakura Film Removal In-Reply-To: <577D1AFFCE584BB7A91EAE2214AE990E@biopath.local> References: <577D1AFFCE584BB7A91EAE2214AE990E@biopath.local> Message-ID: <4C51A026.49FC.00C8.1@beaumonthospitals.com> We use a 50-50 solution of xylene and acetone. Depending on how long the coverslips have been on, it takes from 20 minutes to an hour for them to come off. If you are not decolorizing and re-staining, don't leave them too long in the solution as the stain will start to come out. Sharon E. Scalise, HTL (ASCP) Histology Supervisor William Beaumont Hospital Royal Oak, MI 48073 248 898-5981 sscalise@beaumonthospitals.com >>> "Paula Lucas" 7/29/2010 3:17 PM >>> Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of H&E slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLashus <@t> pathgroup.com Thu Jul 29 14:43:13 2010 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Thu Jul 29 14:43:16 2010 Subject: [Histonet] Sakura Film Removal In-Reply-To: <4C51A026.49FC.00C8.1@beaumonthospitals.com> References: <577D1AFFCE584BB7A91EAE2214AE990E@biopath.local> <4C51A026.49FC.00C8.1@beaumonthospitals.com> Message-ID: <197CD0B02A81F94994A285C59C8AE05C05CE72A312@pgnexchange.pathgroup.com> We 1st use straight acetone and leave the slide in anywhere from 1 to 3 minutes, then 20 seconds in 50/50 acetone/xylene, then dip in straight xylene. Depending on how long the slide has been coverslipped, the times will need to be adjusted. Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Thursday, July 29, 2010 3:37 PM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura Film Removal We use a 50-50 solution of xylene and acetone. Depending on how long the coverslips have been on, it takes from 20 minutes to an hour for them to come off. If you are not decolorizing and re-staining, don't leave them too long in the solution as the stain will start to come out. Sharon E. Scalise, HTL (ASCP) Histology Supervisor William Beaumont Hospital Royal Oak, MI 48073 248 898-5981 sscalise@beaumonthospitals.com >>> "Paula Lucas" 7/29/2010 3:17 PM >>> Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of H&E slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From carl.hobbs <@t> kcl.ac.uk Thu Jul 29 15:00:57 2010 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Jul 29 15:01:08 2010 Subject: [Histonet] Re: Refrigerate formalin? Message-ID: <11D9615B89C10747B1C985966A63D7CA2D7BAE9990@KCL-MAIL04.kclad.ds.kcl.ac.uk> Hey, Sara! Congratulations. Enjoy all them Hassles and Advantages in your NEW lab. Best wishes, Carl NB: re Formalin fixation...one could argue that one should store such-fixed specimens in 90% alcohol, after the appropriate Formalin fixation time? OR...30% Sucrose: now, THAT is a PRESERVATIVE and NOT a Fixative ;-) However....loadsa variables. For eg: ER/PR Ab immunolocalisation ..I understand that , for Clinical Labs ,there is a "window " of acceptible Formalin fixation? So, if we store specimens in Formalin after diagnosis....all them friggin defined/optimised conditions are OUT of the window? NB: why refrigerate Formalin? It will do the same thing but, slower. Stickthem tissues into 30% Sucrose? Well, nobody knows anything rigorous re actuality of Formalin fixation. Still...;-) Empirically, Carl From thomas.crowell <@t> novartis.com Thu Jul 29 15:27:14 2010 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Thu Jul 29 15:27:35 2010 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 07/29/2010 and will not return until 08/02/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From brian1975 <@t> email.com Thu Jul 29 16:07:42 2010 From: brian1975 <@t> email.com (brian1975@email.com) Date: Thu Jul 29 16:08:08 2010 Subject: [Histonet] HTL exam Message-ID: <8CCFD7938129234-1368-D450@web-mmc-d06.sysops.aol.com> Does anyone have any advice on good study aids, areas of prep to concentrate on, or any test taking strategies that helped them? I have been pouring over the 3rd ed of "histotechnology, a self instructional text" for months but since I have started to look at the ASCP/ NSH discussion boards im getting the feeling that it is just not enough. Thanks for any help. -Brian From rabe_brian <@t> yahoo.com Thu Jul 29 17:14:12 2010 From: rabe_brian <@t> yahoo.com (Brian Rabe) Date: Thu Jul 29 17:14:17 2010 Subject: [Histonet] Coverslipping Problem - Please Help! Message-ID: <190348.10017.qm@web111506.mail.gq1.yahoo.com> Dear Histonet members, I have been cryosectioning Xenopus laevis embryos this summer that have undergone a whole mount chromogenic in situ. The embryos are then fixed overnight in a formaldehyde solution (1X MEMFA) at 4 C, then stored in 1 x PBS until they are cryoprotected in a sucrose solution at least overnight at 4 C. Then they are put in TBS tissue freezing medium for 2-3 hours at room temperature before being frozen and sectioned. The sections come out looking very nice with good morphology, but when they are coverslipped (2 1 minute washes in 1 x PBS to dissolve the tissue freezing medium) with VectaMount AQ aqueous mounting medium, the sections turn very dark under the microscope, especially the gut region. They are so dark that it becomes difficult to see the signal from the in situ. Any help or advice would be GREATLY appreciated! Thank you for your time, Brian Rabe The College of William and Mary From Timothy.Morken <@t> ucsfmedctr.org Thu Jul 29 17:32:34 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Jul 29 17:32:53 2010 Subject: [Histonet] HTL exam In-Reply-To: <8CCFD7938129234-1368-D450@web-mmc-d06.sysops.aol.com> References: <8CCFD7938129234-1368-D450@web-mmc-d06.sysops.aol.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C023D4825B3@EXMBMCB15.ucsfmedicalcenter.org> Brian, What helped me a lot with stains, fixatives, etc, was to make a chart of each of the stain or fixative "families" (silver, trichromes, etc) and list the method steps of each, components used, and purpose of the components. That put in perspective the reasons for the differences, which are mysteries otherwise! I also used the NSH study guides, and any other book or study guide I could find to refer to. I was also lucky that I had a group of four people who were studying for the test and we spent a YEAR in a once-weekly study group going through each chapter in detail (Sheehan at that time). That was great for motivation and staying on track. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of brian1975@email.com Sent: Thursday, July 29, 2010 2:08 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL exam Does anyone have any advice on good study aids, areas of prep to concentrate on, or any test taking strategies that helped them? I have been pouring over the 3rd ed of "histotechnology, a self instructional text" for months but since I have started to look at the ASCP/ NSH discussion boards im getting the feeling that it is just not enough. Thanks for any help. -Brian _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Jul 29 17:52:11 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Jul 29 17:52:29 2010 Subject: [Histonet] Trying to locate metal slide racks for 19 slides Message-ID: <000001cb2f70$af948560$0ebd9020$@callis@bresnan.net> Histonetters, It has been so long since we had to order these slide racks, I am can only hope they are still made/available. Lipshaw(that's digging into the past!) / Shandon / ThermoShandon used to carry them but not seeing them on Thermo Scientific website. What has been found are metal racks with slanted sides, but those are unacceptable. The racks are stainless steel with straight sides, detachable handle, and hold 19(20?) slides in horizontal position. Handles are detachable. I think the last supplier who had these was RA Lamb, now part of Thermo Scientific but not finding the racks on Thermo's website. So far a search has not located straight sided racks for 19(20) slides - only slanted side racks. A supplier would be greatly appreciated. Also, who still carries the 20 slide capacity staining dishes? Thanks, Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 From ccrowder <@t> vetmed.lsu.edu Thu Jul 29 23:51:48 2010 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Jul 29 23:56:03 2010 Subject: [Histonet] Reticulum stain wash-offs Message-ID: Rather than use Halt (or other adhesive) in your water bath, have you tried using charged slides - those used for IHC. Then air-dry or heat dry the tissues before deparaffinizing. Any water left in the tissue will cause wash-offs. Do not lower the pH of the silver solution. Cheryl Crowder, BA, HTL(ASCP) From kfineout <@t> hotmail.com Fri Jul 30 06:49:53 2010 From: kfineout <@t> hotmail.com (Kelly Larson) Date: Fri Jul 30 06:49:59 2010 Subject: [Histonet] shopping is online. Message-ID: Discover the latest in women's and men's fashion from brand new cheap goods online with. www.shopbei.com With over 35,000 products from 46 brands, ROBBUY brings you the best in fashion online. New In: Clothing, Shoes(Sport shoes, casual shoes,leather shoes,high heels), Handbags, T shirts, Watches, Jewelry Shoppers can also save money by 1. Register as the VIP member, get 15% discount. It is free to be our member. 2. For the promotion goods, can get 20% discount. Shop here for thousands of cheap brand products from www.shopbei.com, it's safe & easy! From tanisha.neely <@t> covance.com Fri Jul 30 09:02:24 2010 From: tanisha.neely <@t> covance.com (Neely, Tanisha) Date: Fri Jul 30 09:02:46 2010 Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? Message-ID: <816E3C72F855F14985FC31D7C963AE6F205A623D@indexch03.ent.covance.com> Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From tjasper <@t> copc.net Fri Jul 30 10:59:20 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Jul 30 10:59:27 2010 Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? References: <816E3C72F855F14985FC31D7C963AE6F205A623D@indexch03.ent.covance.com> Message-ID: <90354A475B420441B2A0396E5008D49692BF82@copc-sbs.COPC.local> Hi Tanisha, I think the question might be...who's requirements are you referring to. I see by the header you reference NY state. I see by your signature that you are in Indiana. I am not aware of any CAP reg referencing this and, to my knowledge most labs have their own job descriptions written with their own qualifications listed. You say "...right now I'm being told..." so again, by who (whom). You also reference a BA, I'm assuming this means BS or BAS works as well. I personally know very good supervisors that are associate degreed and hold HTs. I also know supervisors that have bachelor's degrees and I wouldn't necessarily say that they are any better because of it, however I think that's a "truism" across a lot of occupations. Just trying to understand your question. Have a good day. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Neely, Tanisha Sent: Friday, July 30, 2010 7:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From dholmes <@t> umc.edu Fri Jul 30 11:37:52 2010 From: dholmes <@t> umc.edu (Dianne E. Holmes) Date: Fri Jul 30 11:38:11 2010 Subject: [Histonet] staining & charged slides Message-ID: Should charged slides be used for 25 micron sections going thru Nissl stain process? I have used this process on 50 micron sections mounted on gelatin-subbed slides and it worked fine. This time the investigator wanted thinner slides which promptly fell off in the staining dish!! What alternatives do I have for processing this tissue? HELP !!!!! Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From kadamsplw <@t> gmail.com Fri Jul 30 12:17:45 2010 From: kadamsplw <@t> gmail.com (karen adams) Date: Fri Jul 30 12:17:49 2010 Subject: [Histonet] problems with FISH Message-ID: Hello all............. I am encountering a smudgy/milky apearance to my urovision FISH slides.....not all in the batch are that way......I know my temps and times were all go and have pH reagents again ....the only variables I can not acct for are 1. my alcohol. Suppose to use 100% Ethanol w/ no methanol in it.....vendor sent "reagent grade" anhydrous and 2. maybe did not incorporate agitation ? any help or advice would be greatly appreciated. -- K. Leigh Adams, MS, HTL (ASCP),SLS Tennessee Urology Associates 800 Oak Ridge TPKE/STE A206 Oak Ridge, TN 37830 (865) 483-1800 kadamsplw@gmail.com From brett_connolly <@t> merck.com Fri Jul 30 12:33:36 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Jul 30 12:33:39 2010 Subject: [Histonet] FW: anti-Ly-6G (aka GR-1 ) IHC question Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D70793A1@usctmx1176.merck.com> Resending - > Does anyone have experience with any anti-mouse LY-6G antibodies on > FFPE mouse tissue for staining neutrophils?? > > Looking for antibodies and protocol suggestions. > > Thanks, > Brett > > Brett M. Connolly, Ph.D. > Molecular Imaging Team Leader > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From collette2 <@t> llnl.gov Fri Jul 30 13:02:03 2010 From: collette2 <@t> llnl.gov (Collette, Nicole M.) Date: Fri Jul 30 13:02:08 2010 Subject: [Histonet] strontium vs. calcium in bone Message-ID: <3AD1C8AEFB40D7408467F3A333A8A6D1019A37F80B4E@NSPEXMBX-B.the-lab.llnl.gov> Hi, Everyone, Happy Friday! I was wondering if anyone knows of a histological method to distinguish strontium in bone vs. calcium? My understanding is that mineral stains (von Kossa or Alizarin Red S) do not distinguish, but was wondering if anyone has a procedure I may not have done or seen? I have ways to get at the answer, but for completeness sake, I thought I should ask the experts. Thanks for your help! Sincerely, Nicole Collette LLNL/ UC Berkeley From liz <@t> premierlab.com Fri Jul 30 13:03:12 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jul 30 13:02:37 2010 Subject: [Histonet] FW: anti-Ly-6G (aka GR-1 ) IHC question In-Reply-To: <9FE33752FA3F3647BC85BCDC3EA6C3D70793A1@usctmx1176.merck.com> Message-ID: Brett We have not used that antibody but we have used the following antibody from Serotec with good success. Protocol: Mouse Neutrophil Immunohistochemical Stain Clone: 7/4 Vendor: Serotec Catalog Number: MCA771 Species: Mouse Isotype: Rat IgG2a Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, July 30, 2010 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: anti-Ly-6G (aka GR-1 ) IHC question Resending - > Does anyone have experience with any anti-mouse LY-6G antibodies on > FFPE mouse tissue for staining neutrophils?? > > Looking for antibodies and protocol suggestions. > > Thanks, > Brett > > Brett M. Connolly, Ph.D. > Molecular Imaging Team Leader > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From collette2 <@t> llnl.gov Fri Jul 30 13:12:25 2010 From: collette2 <@t> llnl.gov (Collette, Nicole M.) Date: Fri Jul 30 13:12:31 2010 Subject: [Histonet] Coverslipping Problem - Please Help! In-Reply-To: <190348.10017.qm@web111506.mail.gq1.yahoo.com> References: <190348.10017.qm@web111506.mail.gq1.yahoo.com> Message-ID: <3AD1C8AEFB40D7408467F3A333A8A6D1019A37F80B56@NSPEXMBX-B.the-lab.llnl.gov> Hi, Brian, It is likely to be a pH issue. If you use BM Purple as your detection chromogen (which is what we use in our lab for whole mounts on X. laevis and X.tropcialis, and mouse embryos for WMISH), then you must keep the embryos at acid pH from BM Purple step onwards, or you will re-activate the BM Purple (hint: Alkaline phosphatase substrate). Light from the microscope compounds your problem. You are likely cryoprotecting and/or coverslipping with neutral or slightly alkaline solution, if you use something acidic, you should be fine. You can adjust the pH of your sucrose pretty readily, so that's not too hard. If you are only coverslipping temporarily for photos (not for archiving), you can simply use a glycerol/PBS pH 5.5 mixture for photos, then discard your slides. Coverslipping for archiving might be a question better addressed my someone else on histonet who is more knowledgeable about mounting media. Good luck! Sincerely, Nicole Collette LLNL/UC Berkeley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brian Rabe Sent: Thursday, July 29, 2010 3:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipping Problem - Please Help! Dear Histonet members, I have been cryosectioning Xenopus laevis embryos this summer that have undergone a whole mount chromogenic in situ. The embryos are then fixed overnight in a formaldehyde solution (1X MEMFA) at 4 C, then stored in 1 x PBS until they are cryoprotected in a sucrose solution at least overnight at 4 C. Then they are put in TBS tissue freezing medium for 2-3 hours at room temperature before being frozen and sectioned. The sections come out looking very nice with good morphology, but when they are coverslipped (2 1 minute washes in 1 x PBS to dissolve the tissue freezing medium) with VectaMount AQ aqueous mounting medium, the sections turn very dark under the microscope, especially the gut region. They are so dark that it becomes difficult to see the signal from the in situ. Any help or advice would be GREATLY appreciated! Thank you for your time, Brian Rabe The College of William and Mary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://*lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Jul 30 13:24:54 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Jul 30 13:24:58 2010 Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F205A623D@indexch03.ent.covance.com> References: <816E3C72F855F14985FC31D7C963AE6F205A623D@indexch03.ent.covance.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640331F427B5@CHEXCMS10.one.ads.che.org> Perhaps someone is thinking about CLIA for which the pathologist meets the requirements for "supervisor" because they do the diagnosing. Most facilities set their own requirements. which would most likely require an HT (HTL preferred) and so many years experience, supervisory experience preferred. This allows for more flexibility in hiring. Hope this helps, Joyce ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Neely, Tanisha [tanisha.neely@covance.com] Sent: Friday, July 30, 2010 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From mark.hocking <@t> ventana.roche.com Fri Jul 30 13:47:52 2010 From: mark.hocking <@t> ventana.roche.com (Hocking, Mark) Date: Fri Jul 30 13:48:32 2010 Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 36 Message-ID: Q Mark Hocking Symphony Technical Applications Specialist Ventana Medical Systems, Inc. A member of the Roche group Message sent via blackberry ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Fri Jul 30 13:02:57 2010 Subject: Histonet Digest, Vol 80, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Reticulum stain wash-offs (Cheryl Crowder) 2. shopping is online. (Kelly Larson) 3. Histology Lab Supervisor Requirements - New York State/CAP Regula tions? (Neely, Tanisha) 4. RE: Histology Lab Supervisor Requirements - New York State/CAP Regula tions? (Thomas Jasper) 5. staining & charged slides (Dianne E. Holmes) ---------------------------------------------------------------------- Message: 1 Date: Thu, 29 Jul 2010 23:51:48 -0500 From: "Cheryl Crowder" Subject: [Histonet] Reticulum stain wash-offs To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Rather than use Halt (or other adhesive) in your water bath, have you tried using charged slides - those used for IHC. Then air-dry or heat dry the tissues before deparaffinizing. Any water left in the tissue will cause wash-offs. Do not lower the pH of the silver solution. Cheryl Crowder, BA, HTL(ASCP) ------------------------------ Message: 2 Date: Fri, 30 Jul 2010 07:49:53 -0400 From: Kelly Larson Subject: [Histonet] shopping is online. To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Discover the latest in women's and men's fashion from brand new cheap goods online with. www.shopbei.com With over 35,000 products from 46 brands, ROBBUY brings you the best in fashion online. New In: Clothing, Shoes(Sport shoes, casual shoes,leather shoes,high heels), Handbags, T shirts, Watches, Jewelry Shoppers can also save money by 1. Register as the VIP member, get 15% discount. It is free to be our member. 2. For the promotion goods, can get 20% discount. Shop here for thousands of cheap brand products from www.shopbei.com, it's safe & easy! ------------------------------ Message: 3 Date: Fri, 30 Jul 2010 10:02:24 -0400 From: "Neely, Tanisha" Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? To: histonet@lists.utsouthwestern.edu Message-ID: <816E3C72F855F14985FC31D7C963AE6F205A623D@indexch03.ent.covance.com> Content-Type: text/plain; charset="us-ascii" Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ------------------------------ Message: 4 Date: Fri, 30 Jul 2010 08:59:20 -0700 From: "Thomas Jasper" Subject: RE: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? To: "Neely, Tanisha" Cc: histonet@lists.utsouthwestern.edu Message-ID: <90354A475B420441B2A0396E5008D49692BF82@copc-sbs.COPC.local> Content-Type: text/plain; charset="us-ascii" Hi Tanisha, I think the question might be...who's requirements are you referring to. I see by the header you reference NY state. I see by your signature that you are in Indiana. I am not aware of any CAP reg referencing this and, to my knowledge most labs have their own job descriptions written with their own qualifications listed. You say "...right now I'm being told..." so again, by who (whom). You also reference a BA, I'm assuming this means BS or BAS works as well. I personally know very good supervisors that are associate degreed and hold HTs. I also know supervisors that have bachelor's degrees and I wouldn't necessarily say that they are any better because of it, however I think that's a "truism" across a lot of occupations. Just trying to understand your question. Have a good day. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Neely, Tanisha Sent: Friday, July 30, 2010 7:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ------------------------------ Message: 5 Date: Fri, 30 Jul 2010 11:37:52 -0500 From: "Dianne E. Holmes" Subject: [Histonet] staining & charged slides To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Should charged slides be used for 25 micron sections going thru Nissl stain process? I have used this process on 50 micron sections mounted on gelatin-subbed slides and it worked fine. This time the investigator wanted thinner slides which promptly fell off in the staining dish!! What alternatives do I have for processing this tissue? HELP !!!!! Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 36 **************************************** From mark.hocking <@t> ventana.roche.com Fri Jul 30 13:47:57 2010 From: mark.hocking <@t> ventana.roche.com (Hocking, Mark) Date: Fri Jul 30 13:48:35 2010 Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 36 Message-ID: Q Mark Hocking Symphony Technical Applications Specialist Ventana Medical Systems, Inc. A member of the Roche group Message sent via blackberry ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Fri Jul 30 13:02:57 2010 Subject: Histonet Digest, Vol 80, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Reticulum stain wash-offs (Cheryl Crowder) 2. shopping is online. (Kelly Larson) 3. Histology Lab Supervisor Requirements - New York State/CAP Regula tions? (Neely, Tanisha) 4. RE: Histology Lab Supervisor Requirements - New York State/CAP Regula tions? (Thomas Jasper) 5. staining & charged slides (Dianne E. Holmes) ---------------------------------------------------------------------- Message: 1 Date: Thu, 29 Jul 2010 23:51:48 -0500 From: "Cheryl Crowder" Subject: [Histonet] Reticulum stain wash-offs To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Rather than use Halt (or other adhesive) in your water bath, have you tried using charged slides - those used for IHC. Then air-dry or heat dry the tissues before deparaffinizing. Any water left in the tissue will cause wash-offs. Do not lower the pH of the silver solution. Cheryl Crowder, BA, HTL(ASCP) ------------------------------ Message: 2 Date: Fri, 30 Jul 2010 07:49:53 -0400 From: Kelly Larson Subject: [Histonet] shopping is online. To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Discover the latest in women's and men's fashion from brand new cheap goods online with. www.shopbei.com With over 35,000 products from 46 brands, ROBBUY brings you the best in fashion online. New In: Clothing, Shoes(Sport shoes, casual shoes,leather shoes,high heels), Handbags, T shirts, Watches, Jewelry Shoppers can also save money by 1. Register as the VIP member, get 15% discount. It is free to be our member. 2. For the promotion goods, can get 20% discount. Shop here for thousands of cheap brand products from www.shopbei.com, it's safe & easy! ------------------------------ Message: 3 Date: Fri, 30 Jul 2010 10:02:24 -0400 From: "Neely, Tanisha" Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? To: histonet@lists.utsouthwestern.edu Message-ID: <816E3C72F855F14985FC31D7C963AE6F205A623D@indexch03.ent.covance.com> Content-Type: text/plain; charset="us-ascii" Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ------------------------------ Message: 4 Date: Fri, 30 Jul 2010 08:59:20 -0700 From: "Thomas Jasper" Subject: RE: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? To: "Neely, Tanisha" Cc: histonet@lists.utsouthwestern.edu Message-ID: <90354A475B420441B2A0396E5008D49692BF82@copc-sbs.COPC.local> Content-Type: text/plain; charset="us-ascii" Hi Tanisha, I think the question might be...who's requirements are you referring to. I see by the header you reference NY state. I see by your signature that you are in Indiana. I am not aware of any CAP reg referencing this and, to my knowledge most labs have their own job descriptions written with their own qualifications listed. You say "...right now I'm being told..." so again, by who (whom). You also reference a BA, I'm assuming this means BS or BAS works as well. I personally know very good supervisors that are associate degreed and hold HTs. I also know supervisors that have bachelor's degrees and I wouldn't necessarily say that they are any better because of it, however I think that's a "truism" across a lot of occupations. Just trying to understand your question. Have a good day. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Neely, Tanisha Sent: Friday, July 30, 2010 7:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ------------------------------ Message: 5 Date: Fri, 30 Jul 2010 11:37:52 -0500 From: "Dianne E. Holmes" Subject: [Histonet] staining & charged slides To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Should charged slides be used for 25 micron sections going thru Nissl stain process? I have used this process on 50 micron sections mounted on gelatin-subbed slides and it worked fine. This time the investigator wanted thinner slides which promptly fell off in the staining dish!! What alternatives do I have for processing this tissue? HELP !!!!! Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 36 **************************************** From MLashus <@t> pathgroup.com Fri Jul 30 13:59:45 2010 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Fri Jul 30 13:59:49 2010 Subject: [Histonet] Slide Imaging/Digital Pathology Message-ID: <197CD0B02A81F94994A285C59C8AE05C05CE72A326@pgnexchange.pathgroup.com> Would anyone be willing to share their procedures/protocols for slide imaging/digital pathology as it relates to CAP checklist questions? Any help would be appreciated. Thanks, Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From akbitting <@t> geisinger.edu Fri Jul 30 14:26:01 2010 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Jul 30 14:26:20 2010 Subject: [Histonet] Parvovirus B19 Message-ID: <4C52EF08.2B7F.00C9.0@geisinger.edu> Wondering if anyone is running Cellmarques Parvovirus B19 or Toxoplasma Gondii on a Ventana platform and would you mind sharing your protocol? Have a great weekend everyone! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From zodiac29 <@t> comcast.net Fri Jul 30 15:07:33 2010 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Fri Jul 30 15:07:36 2010 Subject: [Histonet] filing unstained slides Message-ID: <1792593272.822531.1280520453555.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> To All, Just wanted to know how other labs are filing their unstained slides?? If filing along with stained coverslipped slides wouldn't the sections on the unstained slides get scratched?? Any input is appreciated. Thanks in advance, Jenny From Maria.Katleba <@t> stjoe.org Fri Jul 30 15:14:07 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Fri Jul 30 15:20:15 2010 Subject: [Histonet] filing unstained slides In-Reply-To: <1792593272.822531.1280520453555.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> References: <1792593272.822531.1280520453555.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: We file them along with the regular H&E.... usually used when we have send out or re-cut requests. They rarely get scratched (but you must handle them with care). Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net Sent: Friday, July 30, 2010 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] filing unstained slides To All, Just wanted to know how other labs are filing their unstained slides?? If filing along with stained coverslipped slides wouldn't the sections on the unstained slides get scratched?? Any input is appreciated. Thanks in advance, Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From MLashus <@t> pathgroup.com Fri Jul 30 15:25:00 2010 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Fri Jul 30 15:25:05 2010 Subject: [Histonet] Adenovirus controls Message-ID: <197CD0B02A81F94994A285C59C8AE05C05CE72A328@pgnexchange.pathgroup.com> I am looking for adenovirus controls, would anyone know where I could get some? I have checked Newcomer and ThermoFisher and they are out. Thanks, Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From wdesalvo.cac <@t> hotmail.com Fri Jul 30 15:33:29 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Jul 30 15:33:36 2010 Subject: [Histonet] filing unstained slides In-Reply-To: <1792593272.822531.1280520453555.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> References: <1792593272.822531.1280520453555.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: We cut unstained slides for process efficiency and keep the unstained slides separate form all other stained slides. The unstained slides are kept no longer than 3 months and then discarded. We do not place any slides in the permanent file that has not been reviewed by a pathologist. William DeSalvo, B.S., HTL(ASCP) > Date: Fri, 30 Jul 2010 20:07:33 +0000 > From: zodiac29@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] filing unstained slides > > To All, > > > Just wanted to know how other labs are filing their unstained slides?? If filing along with stained coverslipped slides wouldn't the sections on the unstained slides get scratched?? Any input is appreciated. > > > Thanks in advance, > > > Jenny > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From Rcartun <@t> harthosp.org Fri Jul 30 15:42:37 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jul 30 15:42:46 2010 Subject: [Histonet] filing unstained slides In-Reply-To: <1792593272.822531.1280520453555.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> References: <1792593272.822531.1280520453555.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: <4C5300FD.7400.0077.1@harthosp.org> We file all of our unstained slides (after heating in a 60 degree oven for 30 minutes) with our IHC slides. We frequently go back and do IHC on unstains and I have not noticed any tissue damage. Obviously, care must be taken when filing to prevent tissue damage. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 7/30/2010 4:07 PM >>> To All, Just wanted to know how other labs are filing their unstained slides?? If filing along with stained coverslipped slides wouldn't the sections on the unstained slides get scratched?? Any input is appreciated. Thanks in advance, Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RyanDK307 <@t> aol.com Fri Jul 30 16:02:30 2010 From: RyanDK307 <@t> aol.com (RyanDK307@aol.com) Date: Fri Jul 30 16:02:49 2010 Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 36/Reticulin Stain wash-offs Message-ID: Date: Friday 7/30/10 From: K Ryan _RyanDK307@aol.com_ (mailto:RyanDK307@aol.com) Subject: [Histonet] Reticulum stain wash-offs To: _histonet@lists.utsouthwestern.edu_ (mailto:histonet@lists.utsouthwestern.edu) I found that shortening the time in the potassium permanganate solved my tissue wash off problem. I was staining liver sections cut at 4 microns on "plus"slides. From jackie.puma <@t> sterlingpath.com Fri Jul 30 18:34:48 2010 From: jackie.puma <@t> sterlingpath.com (J Puma) Date: Fri Jul 30 18:34:52 2010 Subject: [Histonet] Job Opportunities Message-ID: <61030.28365.qm@web1003.biz.mail.sp1.yahoo.com> ? Sterling Pathology ?located in Seal Beach California is currently seeking?a Full Time Histotech and (2) Lab Assistants?for Part Time or per diem. Histotech?should be ASCP Certified with at least 3 years experience preferably proficient in immunos.? Lab Assistants may have histology experience preferably with flow cytometry and cytogenetic?but will consider applicants with no lab experience. For immediate consideration please email resume to jackie.puma@sterlingpath.com or fax to (562) 799.5544. From jcampbell <@t> vdxpathology.com Sat Jul 31 14:03:39 2010 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Sat Jul 31 14:03:44 2010 Subject: [Histonet] Help with Biocare polymer detection kit!! Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FF1@VDXSERVER01.vdxpathology.local> I have been trying to get the Mach 1 polymer kit from Biocare to work on my K9 and feline tissue and I am getting terrible nonspecific staining in my positive and negative controls. It appears to be sticking to something in the glands of the intestinal epithelia. I am also getting nonspecific staining in my LN tissue. I decided to switch over to polymer detection kit because I had been dealing with the issue of endogenous biotin when using the streptavidin biotin-based detection system, and now I am getting this other nonspecific staining! I have tried doing runs eliminating my protein block and eliminating the negative control and primary antibody step altogether (to see if maybe the protein block or the diluent used in my negative control and antibody dilutions are causing the problem). I have also reduced the incubation time of both the probe and polymer from 15 min and 30 min, respectively, to 10 min each. I have tried cutting down my antigen retrieval time substantially as well. I'm not sure what else could be causing this. Any suggestions?? Thanks in advance, Jennifer Campbell