From baustin <@t> cbgbiotech.com Fri Jan 1 12:06:28 2010 From: baustin <@t> cbgbiotech.com (Baustin) Date: Fri Jan 1 12:06:34 2010 Subject: [Histonet] Auto Reply Message-ID: <1008822478@mail-server.cbgbiotech.com> Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. From pruegg <@t> ihctech.net Fri Jan 1 12:22:17 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jan 1 12:22:58 2010 Subject: SPAM-LOW: [Histonet] Re: Stain to differentiate Hemoglobin from Hemosiderin? In-Reply-To: References: Message-ID: I agree with Samurai, Prussian Blue will only stain iron, it is very specific and will not stain the other components listed. I have used it to verify aluminum staining reaction in bone, sometimes it is iron and not really aluminum, but the aluminum reaction I used would stain aluminum and iron, the Prussian blue reaction would not stain aluminum, only iron, so we had to do the two together to verify that the positive al staining was really AL and not iron deposited in the bone, this happens to renal patients on dialysis when they cannot metabolize out the AL it deposits at the surface of the bone and can prevent formation of new bone, and there are some diseases where iron is deposited in the bone the same way AL is. Happy New Year! Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Tuesday, December 29, 2009 3:26 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: Stain to differentiate Hemoglobin from Hemosiderin? Jerry Ricks, Research Scientist, University of Washington,Department of Pathology asks: >>I've seen Prussian blue as a stain for hemosiderin but that would also stain hemoglobin.<< The Perls prussian blue reaction occurs with hemosiderin ("stainable iron") but not with hemoglobin, hematin, formalin pigment, malarial pigment, or melanin. It isn't a stain, but a reaction between ferric iron and ferrocyanide ion that produces a dense blue precipitate. The Stainsfile page doesn't really answer your questions, and I'd suggest looking it up in some of the standard textbooks. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjay30 <@t> yahoo.com Fri Jan 1 13:21:29 2010 From: tjay30 <@t> yahoo.com (Tim) Date: Fri Jan 1 13:21:32 2010 Subject: [Histonet] Derm lab set up Message-ID: <111121.10387.qm@web34308.mail.mud.yahoo.com> You can contact Pillar Consulting to set up your derm or Gi path lab. 775-830-1591. Sent from my iPhone From rsrichmond <@t> gmail.com Fri Jan 1 14:40:26 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Jan 1 14:40:30 2010 Subject: [Histonet] Re: FNA code Message-ID: Vinnie Della Speranza notes: >>a resource your dept might find very beneficial entitled "Pathology Service Coding Handbook" which is authored by Dennis Padget, a noted coding authority.<< I can Google references to Dennis L. Padget's "Pathology Service Coding Handbook", but I cannot find out how to buy it. It may be a handout at high-priced coding conferences, and not available in the book trade at all. In my locum tenens practice I am often called on to do CPT coding, usually without so much as the AMA's annual book by way of references. I find most small pathology services nearly clueless about CPT coding. I typically hear from the staff about how careful Dr. Smith is about billing for everything, and then I find out he's hemorrhaging money by undercoding. Bob Richmond Samurai Pathologist Knoxville TN From JWeems <@t> sjha.org Fri Jan 1 20:24:28 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Jan 1 20:26:32 2010 Subject: [Histonet] Re: FNA code References: Message-ID: It is an expensive resource, but a good one. It is difficult to code accurately but is so important so as not to be misconstrued as fraud! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robert Richmond Sent: Fri 1/1/2010 3:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: FNA code Vinnie Della Speranza notes: >>a resource your dept might find very beneficial entitled "Pathology Service Coding Handbook" which is authored by Dennis Padget, a noted coding authority.<< I can Google references to Dennis L. Padget's "Pathology Service Coding Handbook", but I cannot find out how to buy it. It may be a handout at high-priced coding conferences, and not available in the book trade at all. In my locum tenens practice I am often called on to do CPT coding, usually without so much as the AMA's annual book by way of references. I find most small pathology services nearly clueless about CPT coding. I typically hear from the staff about how careful Dr. Smith is about billing for everything, and then I find out he's hemorrhaging money by undercoding. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From jcbook <@t> gmail.com Mon Jan 4 04:37:37 2010 From: jcbook <@t> gmail.com (J C) Date: Mon Jan 4 04:37:43 2010 Subject: [Histonet] polyester wax Message-ID: <52cb0f131001040237o3ff6a270v5b5f40ccf67f277d@mail.gmail.com> Dear Histonetters! Does somebody use polyester wax for embedding? I need it for hydrogel that disappears in hot paraplast. Can you advice any manufacture to me? \Thanks, Julie Carmel, Haifa, Israel From ogv_histech <@t> yahoo.com Mon Jan 4 05:15:05 2010 From: ogv_histech <@t> yahoo.com (oscar gonzalez) Date: Mon Jan 4 05:15:08 2010 Subject: [Histonet] CAP regs Message-ID: <324684.71834.qm@web53504.mail.re2.yahoo.com> Does anyone have any protocols to comply with the new CAP regulations regardng imaging? From meghan.tucker <@t> yahoo.com Mon Jan 4 09:23:01 2010 From: meghan.tucker <@t> yahoo.com (Meghan Tucker) Date: Mon Jan 4 09:23:07 2010 Subject: [Histonet] Cutting Frozen Lung Message-ID: <542726.89047.qm@web113209.mail.gq1.yahoo.com> Hello! ? I am looking for some helpful hints for cutting frozen lung tissue. I have tried just about everything I can think of! ? Thanks! From Wanda.Smith <@t> HCAhealthcare.com Mon Jan 4 09:25:08 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Mon Jan 4 09:25:14 2010 Subject: [Histonet] Problems with Denatured Alcohol? Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13808AEB17@NADCWPMSGCMS03.hca.corpad.net> Happy New Year to Everyone!!!!! We recently changed from Reagent Alcohol to Denatured Alcohol as a cost saving measure per the "bean counters". The cutting histotechs have started complaining that the tissue seems dry and they are having difficulty cutting sections. Has anyone else had this problem and are there any adjustments in processing times, etc that may help the situation??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From gvdobbin <@t> ihis.org Mon Jan 4 09:43:47 2010 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Jan 4 09:44:08 2010 Subject: [Histonet] Cutting Frozen Lung Message-ID: Hi Meghan, Healthy lung is terrible to cut frozen. All those air spaces collapse on sectioning. Unhealthy lung on the other hand is a dream to cut! I have heard it suggested to perfuse the lungs with dilute frozen cutting compound (eg OCT or similar) prior to selecting the piece(s) for frozen sectioning. In theory it should work well but I have not tried it myself. Otherwise, a quick swipe of the cutting surface with your thumb just before sectioning might help a little (maximizing the amount of viewable tissue in the section) but your sections (of healthy lung)will never be "beautiful" in my experience. good luck. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Meghan Tucker 1/4/2010 11:23:01 AM >>> Hello! I am looking for some helpful hints for cutting frozen lung tissue. I have tried just about everything I can think of! Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From leiker <@t> buffalo.edu Mon Jan 4 09:48:36 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Jan 4 09:48:42 2010 Subject: [Histonet] Probe for mouse Y chromosome for FISH In-Reply-To: <054452CCC076BE4DA21E46AE95E32EC3277BE9@mail2.asaf.health.gov.il> References: <054452CCC076BE4DA21E46AE95E32EC3277BE9@mail2.asaf.health.gov.il> Message-ID: <1887BCD9D5996569D031C5D5@CDYwxp1931.ad.med.buffalo.edu> Cambio does sell it, I've used their probes. OpenBiosystems is their US distributor. But it still takes 4-6 weeks (minus a hold up in customs) just to get probes to the US...not sure about Israel. We are looking at ordering from ID Labs, a company in London, Ontario (Canada), which is much closer to home for us. They also supply protocols for both frozen and paraffin sections. Hope this helps you and anyone else interested! I've just started FISH myself so that is basically all I know about suppliers... Regards, Merced --On Wednesday, December 30, 2009 12:17 PM +0200 birnbaumm@asaf.health.gov.il wrote: > Dear all > > We look for Fluorescence probe of mouse Y chromosome. Which company does > sale it? > > Mira Birnbaum > > Pathology > > Asaf Hrofeh Medical Center > > Israel > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Linda.Watson <@t> bms.com Mon Jan 4 09:51:19 2010 From: Linda.Watson <@t> bms.com (Watson, Linda) Date: Mon Jan 4 09:51:26 2010 Subject: [Histonet] Cutting Frozen Lung In-Reply-To: References: Message-ID: <351A66CE7FB11D40AB8AC8FE5559EC7E0230BC15E8@ushpwbmsmmp008.one.ads.bms.com> I always perfuse/inflate my lung samples with dilute OCT before freezing. It does make the cutting much easier and the morphology is pretty good considering that it is a frozen section. Good luck, Linda >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin >Sent: Monday, January 04, 2010 10:44 AM >To: histonet@lists.utsouthwestern.edu; meghan.tucker@yahoo.com >Subject: Re: [Histonet] Cutting Frozen Lung > >Hi Meghan, >Healthy lung is terrible to cut frozen. All those air spaces collapse >on sectioning. Unhealthy lung on the other hand is a dream to cut! I >have heard it suggested to perfuse the lungs with dilute frozen cutting >compound (eg OCT or similar) prior to selecting the piece(s) for frozen >sectioning. In theory it should work well but I have not tried it >myself. Otherwise, a quick swipe of the cutting surface with your thumb >just before sectioning might help a little (maximizing the amount of >viewable tissue in the section) but your sections (of healthy lung)will >never be "beautiful" in my experience. >good luck. >Greg > >Greg Dobbin, R.T. >Chief Technologist, Anatomic Pathology >Dept. of Laboratory Medicine, >Queen Elizabeth Hospital, >P.O. Box 6600 >Charlottetown, PE C1A 8T5 >Phone: (902) 894-2337 >Fax: (902) 894-2385 > >"I find that the harder I work, the >more luck I seem to have." >- Thomas Jefferson > > >>>> Meghan Tucker 1/4/2010 11:23:01 AM >>> >Hello! > >I am looking for some helpful hints for cutting frozen lung tissue. I >have tried just about everything I can think of! > >Thanks! > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >------------------------- >Statement of Confidentiality >This message (including attachments) may contain confidential or >privileged information intended for a specific individual or >organization. If you have received this communication in error, please >notify the sender immediately. If you are not the intended recipient, >you are not authorized to use, disclose, distribute, copy, print or rely >on this email, and should promptly delete this email from your entire >computer system. > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From mucram11 <@t> comcast.net Mon Jan 4 09:52:34 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Jan 4 09:52:37 2010 Subject: [Histonet] Problems with Denatured Alcohol? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13808AEB17@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <561517120.49561262620354661.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Wanda, The first thing you need to find out is what did they denature it with.? Denatured alcohol may not be the mixture of isopropanol and methanol in ethanol you were getting and could be chemically denatured.? This is a problem that comes up when we don't check all the variables in what?is actually in the products we buy and the vendor doesn't tell us why it got cheaper.? If it is a chemical denaturing of the alcohol it could be designed for use in one of many fields just not histology.? The real requirement for reagent and denatured alcohol with the ATF is that the product be undrinkable or unfit for human consumption.? Also different manufacturers use varying amounts of isopropanol/methanol/ethanol and that can change the way it processes and stains tissue.? The best is 5% each of isopropanol and methanol with the remaining 90% ethanol. Pam Marcum UAMS ----- Original Message ----- From: "Smith Wanda" To: histonet@lists.utsouthwestern.edu Sent: Monday, January 4, 2010 9:25:08 AM GMT -06:00 US/Canada Central Subject: [Histonet] Problems with Denatured Alcohol? Happy New Year to Everyone!!!!! We recently changed from Reagent Alcohol to Denatured Alcohol as a cost saving measure per the "bean counters". ?The cutting histotechs have started complaining that the tissue seems dry and they are having difficulty cutting sections. ?Has anyone else had this problem and are there any adjustments in processing times, etc that may help the situation??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC ?29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Mon Jan 4 09:54:12 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Jan 4 09:54:17 2010 Subject: [Histonet] RE: IF staining on FFPE tissue In-Reply-To: References: Message-ID: <88A70253A76A2D667AA4F0DF@CDYwxp1931.ad.med.buffalo.edu> Don't know if anyone else replied to this...but maybe try tinkering with your angtigen retrieval? Like try altering the temp, duration, or even the pH? pH can be finicky for some epitopes, I've found... Regards, Merced --On Wednesday, December 23, 2009 12:56 PM -0600 "Margaryan, Naira" wrote: > Dear Histonetters, > > Hopefully you are enjoying a great Holidays with your friends and family! > > I have been asked to perform . I got positive and negative absolutely > identical with beautiful nuclear DAPI and very red RBC. What to do or > what to change in my protocol to avoid RBC and background? Here is my > protocol: 1.Deparaffinization (xyline- 60min, 100%Eth- 15min, 95%- 5min, > 70%- 5min, H2O) 2. Antigen Retrieval ph6 in Citrate buffer (same I use > for IHC) > 3. Wash H2O and TBST > 4. Protein block- 10min > 5. Ab, Rb anti-human - 60-90 min (same I use for IHC) > 6. Wash TBST -5-10min > 7. secondary Donkey anti-Rb 594 red - 60min > 8. Wash TBST -5-10min, H2O -5min > 9. DAPI - 10min > 10. Wash H2O -5-10min > 11. Gelvatol coverslip > > Am I using wrong secondary? Is there any specific secondary or protocol > for IF staining for FFPE to avoid background? I just used same AR and > primary Ab's dilution like i use for IHC with nice results. > > Any suggestions are appreciated, especially in these Holidays days from > working histo- people:) > > Thanks and have a warm and nice Holidays, > Naira > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Fawn.Bomar <@t> HalifaxRegional.com Mon Jan 4 09:08:54 2010 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Mon Jan 4 09:54:34 2010 Subject: [Histonet] Cutting Teeth Message-ID: Hi everyone, I hope you all had A wonderful Holiday. Does anyone have any suggestions on how to soften teeth to cut in paraffin? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From flnails <@t> texaschildrens.org Mon Jan 4 10:51:04 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon Jan 4 10:51:19 2010 Subject: [Histonet] Cutting Frozen Lung In-Reply-To: <542726.89047.qm@web113209.mail.gq1.yahoo.com> References: <542726.89047.qm@web113209.mail.gq1.yahoo.com> Message-ID: You have to infuse the lung with a 50/50 oct sucrose solution prior to freezing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Meghan Tucker Sent: Monday, January 04, 2010 9:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting Frozen Lung Hello! ? I am looking for some helpful hints for cutting frozen lung tissue. I have tried just about everything I can think of! ? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From jamie.erickson <@t> abbott.com Mon Jan 4 12:41:02 2010 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Mon Jan 4 12:41:10 2010 Subject: [Histonet] mouse Cytokine ICC/IHC protocol help. In-Reply-To: References: Message-ID: Hi All, Happy New Year... As the new year is upon us I and my fellow histologist feel compelled to attempt IHC on mouse tissue again for the detection of various cytokines. As I have not had much success in this area in the past I thought I would ask if there is anyone out there that you know that may help us on our Journey.. We want to try to detect cytokine (TNF,IL-1,IL-13) along with our soluble targets in mouse and rat tissues. Ideally we want to use paraffin sections so we will explore fixations/processing schedules..and any other voodoo solutions that might work. If you or someone you know has a good source of papers/ protocol we might download we would love to have them.. As we have the ability to use mice and rats we will be simulating/overexpression of cytokines in-vivo and taking tissues at necropsies in various fixations. If you can help please let me know. Thanks and Happy new Year. Jamie Erickson Abbott Labs. Scientist II, M.S. HTL (ASCP) Discovery Safety, Metabolism & Pharmacokinetics Phone 508-688-3134 FAX 508-793-4895 jamie.erickson@abbott.com From anonwums1 <@t> gmail.com Mon Jan 4 12:53:44 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Mon Jan 4 12:53:48 2010 Subject: [Histonet] mouse Cytokine ICC/IHC protocol help. In-Reply-To: References: Message-ID: <858249121001041053i250a12b3p390f45a27720a2fc@mail.gmail.com> I've managed to get one chemokine (SDF-1) to work on FFPE sections. The key was using the tyramide amplification system from Perkin Elmer. It's a dirty staining because it's secreted so you see staining bound up randomly in noncellular extracellular matrix but you do see it concentrated in some cells. Since it's not easy, our lab tries to avoid doing this type of delicate work and instead do ELISAs and intracellular flow cytometry. Adam On Mon, Jan 4, 2010 at 12:41 PM, Jamie E Erickson wrote: > Hi All, Happy New Year... > > As the new year is upon us I and my fellow histologist feel compelled to > attempt IHC on mouse tissue again for the detection of various cytokines. > As I have not had much success in this area in the past I thought I would > ask if there is anyone out there that you know that may help us on our > Journey.. > > We want to try to detect cytokine (TNF,IL-1,IL-13) along with our soluble > targets in mouse and rat tissues. > Ideally we want to use paraffin sections so we will explore > fixations/processing schedules..and any other voodoo solutions that might > work. > > If you or someone you know has a good source of papers/ protocol we might > download we would love to have them.. > > As we have the ability to use mice and rats we will be > simulating/overexpression of cytokines in-vivo and taking tissues at > necropsies in various fixations. > > If you can help please let me know. > > Thanks and Happy new Year. > > Jamie Erickson > Abbott Labs. > Scientist II, M.S. HTL (ASCP) > Discovery Safety, Metabolism & Pharmacokinetics > Phone 508-688-3134 > FAX 508-793-4895 > jamie.erickson@abbott.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From igor.deyneko <@t> gmail.com Mon Jan 4 13:16:56 2010 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Mon Jan 4 13:17:01 2010 Subject: [Histonet] Decaling mouse joints Message-ID: <35e16a771001041116h923af57ld94ff578d2ac76e8@mail.gmail.com> Dear Histonetters! I have a project coming up which would require to decalcify mouse hind legs, femur and tibia with joints. Can anyone suggest a good system, either an ion exchange or acid decal(formic acid I've heard works well) and also if anyone has a protocol on fixation and decaling, it would be much appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA From liz <@t> premierlab.com Mon Jan 4 13:34:03 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Jan 4 13:34:07 2010 Subject: [Histonet] Decaling mouse joints In-Reply-To: <35e16a771001041116h923af57ld94ff578d2ac76e8@mail.gmail.com> Message-ID: We use formic acid (10%) for decalcification. We fix in 10% NBF for 48 hours. We decal for about 7 days for the mouse joints (ankles, front and rear paws), knees do not take as long. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Igor Deyneko Sent: Monday, January 04, 2010 12:17 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Decaling mouse joints Dear Histonetters! I have a project coming up which would require to decalcify mouse hind legs, femur and tibia with joints. Can anyone suggest a good system, either an ion exchange or acid decal(formic acid I've heard works well) and also if anyone has a protocol on fixation and decaling, it would be much appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pkarlisch <@t> hmc.psu.edu Mon Jan 4 13:44:27 2010 From: pkarlisch <@t> hmc.psu.edu (Patricia Karlisch) Date: Mon Jan 4 13:47:15 2010 Subject: [Histonet] RE: Rapid ISH Message-ID: <4B41FECB.07B7.008C.1@hmc.psu.edu> Hello everyone, Do any of you have experience with Rapid In Situ Hybridization. I believe Biocare has something NEW for rapid ISH staining. Are there other methods out there? Has anyone tried Biocare's Rapid ISH? If you have tried it would you let us know what you think of product. Thank you in advance, Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From w_alkadhumi <@t> yahoo.com Mon Jan 4 12:33:07 2010 From: w_alkadhumi <@t> yahoo.com (wassan alkadhumi) Date: Mon Jan 4 14:00:03 2010 Subject: [Histonet] (no subject) Message-ID: <244633.30120.qm@web45202.mail.sp1.yahoo.com> hi all My name is Wassan am arabic i live in Iraq ,in north of Iraq to be specefic i greduated from scince collage/biotechnology department 2008,i work in a histopathology department?doing basic histopathology and manual immunohistchemistry?as a technician,i have avery supportive superviser who is a professor in histo?that came from?U.S.A he always pushes me to do my best,i like my work very much espacially immunohistochemistry i have so many qustions but most of them i?can google them and find out the answers except this one,i was told never let the slides get dry after deparaffinaization in immunohistochemisrty?why is that?,i red something about corenflake and artifacte,i need more?informations about this please in detal? thanks alot and take care ? Wassan Alkadhumi? technician Histopathology Department Shorsh Hospital Sulemania?????????? From liz <@t> premierlab.com Mon Jan 4 14:12:25 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Jan 4 14:12:28 2010 Subject: [Histonet] (no subject) In-Reply-To: <244633.30120.qm@web45202.mail.sp1.yahoo.com> Message-ID: I believe a cornflake artifact is when your slides are air dried and then coverslipped you may get little refractive tiny grey spots on the slides that look like corn flakes (abnormal shapes). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wassan alkadhumi Sent: Monday, January 04, 2010 11:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) hi all My name is Wassan am arabic i live in Iraq ,in north of Iraq to be specefic i greduated from scince collage/biotechnology department 2008,i work in a histopathology department?doing basic histopathology and manual immunohistchemistry?as a technician,i have avery supportive superviser who is a professor in histo?that came from?U.S.A he always pushes me to do my best,i like my work very much espacially immunohistochemistry i have so many qustions but most of them i?can google them and find out the answers except this one,i was told never let the slides get dry after deparaffinaization in immunohistochemisrty?why is that?,i red something about corenflake and artifacte,i need more?informations about this please in detal? thanks alot and take care ? Wassan Alkadhumi? technician Histopathology Department Shorsh Hospital Sulemania?????????? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Jan 4 14:36:23 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Jan 4 14:37:55 2010 Subject: [Histonet] RE: Rapid ISH In-Reply-To: <4B41FECB.07B7.008C.1@hmc.psu.edu> References: <4B41FECB.07B7.008C.1@hmc.psu.edu> Message-ID: I have had the opportunity to work with the Kappa, Lamda and EBV. Could not be easier. Tech's whom have never run an immuno by hand were doing them on the first day. I believe it is set to be release as an IVD or ASR very very shortly. Any specifics contact me directly. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch [pkarlisch@hmc.psu.edu] Sent: Monday, January 04, 2010 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Rapid ISH Hello everyone, Do any of you have experience with Rapid In Situ Hybridization. I believe Biocare has something NEW for rapid ISH staining. Are there other methods out there? Has anyone tried Biocare's Rapid ISH? If you have tried it would you let us know what you think of product. Thank you in advance, Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcrawfor <@t> aerotek.com Mon Jan 4 14:38:16 2010 From: jcrawfor <@t> aerotek.com (Crawford, Jennifer) Date: Mon Jan 4 14:38:21 2010 Subject: [Histonet] Lead Histotechnologist (Chicago, IL) Message-ID: <571A823E0300F549BE86279A87239577044EE627@ag00-exmbx04.allegisgroup.com> Hello Histonetters, A client of mine in the Chicago area is currently hiring for a Lead Histotechnologist. I have listed the details for this position below and if you are interested I can be reached via email at jcrawfor@aerotek.com or by phone at (847) 221-1358. Histotechnologist Job Description: Under the leadership of the Director and section supervisor, the Histology Technologist will receive and properly process specimens for the Pathologists in a timely manner, reviews test results, and participate in Quality Assurance. The histology technician will also maintain slide files and records, assist the Pathologist and function in a limited capacity as a cytotechnologist as needed. Specific required skills: Immunostaining (Ventana Stainer), Cytology, ASCP certification is mandatory. Shift: Afternoons, Monday through Friday with occasional Saturdays. Pay: $25-30/hr, based on experience Required Skills: * BS IN HISTOLOGY OR RELATED FIELD * ASCP CERTIFICATION * IMMUNOSTAINING EXPERIENCE * CYTOLOGY EXPERIENCE Jen Crawford, CIR Scientific Recruiter Aerotek Scientific Staffing Phone: 847.221.1358 Fax: 847.303.2370 www.aerotek.com Please do not keep me a secret...a referral is the best compliment that I can receive! ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From alyssa <@t> alliedsearchpartners.com Mon Jan 4 15:54:13 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Mon Jan 4 15:54:16 2010 Subject: [Histonet] Job In NY Message-ID: Allied Search Partners is currently accepting resumes in Northern New York, just North of Albany, NY area for qualified Histotechnicians/Histotechnologists Sign On Bonus: Through Allied Search Partners $2000.00 Histotechnologists/Histotechnicians Shifts: Day Shift, 11am-7pm, Full Time, Monday-Friday Benefits: Health, PTO, Sign On Bonus, Relocation Expenses, and much more!! Please submit your resume for prescreening purposes to alyssa@alliedsearchpartners.com *All inquiries are always kept confidential* upon resume submission one of our recruiters will call you for an initial phone interview/confidential phone call. No resume will be submitted before an initial phone interview. Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for $$Cash Bonus$$, and have your resume reviewed by our career advisors. -- Alyssa Peterson Director of Recruitment O: 888.388.7571 x 102 F: 888.388.7572 *Be sure to visit us on the web at www.alliedsearchpartners.com From jnocito <@t> satx.rr.com Mon Jan 4 16:52:28 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Jan 4 16:52:47 2010 Subject: [Histonet] Problems with Denatured Alcohol? In-Reply-To: <561517120.49561262620354661.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <561517120.49561262620354661.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <508BB2F32B7640299E54DF21F88DE279@JoePC> Pam is correct, I mean, look at me, I've been denatured for many years. Happy New Year everyone JTT ----- Original Message ----- From: "Pamela Marcum" To: "Smith Wanda" Cc: Sent: Monday, January 04, 2010 9:52 AM Subject: Re: [Histonet] Problems with Denatured Alcohol? Wanda, The first thing you need to find out is what did they denature it with. Denatured alcohol may not be the mixture of isopropanol and methanol in ethanol you were getting and could be chemically denatured. This is a problem that comes up when we don't check all the variables in what is actually in the products we buy and the vendor doesn't tell us why it got cheaper. If it is a chemical denaturing of the alcohol it could be designed for use in one of many fields just not histology. The real requirement for reagent and denatured alcohol with the ATF is that the product be undrinkable or unfit for human consumption. Also different manufacturers use varying amounts of isopropanol/methanol/ethanol and that can change the way it processes and stains tissue. The best is 5% each of isopropanol and methanol with the remaining 90% ethanol. Pam Marcum UAMS ----- Original Message ----- From: "Smith Wanda" To: histonet@lists.utsouthwestern.edu Sent: Monday, January 4, 2010 9:25:08 AM GMT -06:00 US/Canada Central Subject: [Histonet] Problems with Denatured Alcohol? Happy New Year to Everyone!!!!! We recently changed from Reagent Alcohol to Denatured Alcohol as a cost saving measure per the "bean counters". The cutting histotechs have started complaining that the tissue seems dry and they are having difficulty cutting sections. Has anyone else had this problem and are there any adjustments in processing times, etc that may help the situation??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Tue Jan 5 09:21:12 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Jan 5 09:20:55 2010 Subject: [Histonet] Histotech, Pathologist Assistant, and Cytotech Job Openings Message-ID: <1931295434.1262704871998.JavaMail.cfservice@webserver59> Dear Histonet Members! I hope you are doing well. I am a recruiter at a healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few histotechnician, histotechnologist, cytotechnologist, and pathologist's assistant openings across the country. Our clients typically assist with relocation expenses. One particular client I am working with is looking for a Pathologist's Assistant for a lab in Las Vegas, NV. This lab is looking for someone with experience, PA(ASCP) certified, and someone who either lives in or is willing to relocate to Las Vegas, NV. Las Vegas, Nevada is perhaps one of the most exciting places in the entire country. While most people consider the casino portal to be more of a tourism capital than a long term residency, there are plenty of people who have found an excellent home in this city. Sunshine days usually occur 300 days out of the year, and there are very little rainy days. My client is offering an excellent compensation package, relocation assistance, and full benefits. Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Opportunities: 1. NV - Pathologist's Assistant 2. NV - Flow Cytometry Supervisor 3. CT - Histology Operations Manager 4. Southern CA - Histology Supervisor 5. NY City - Surgical Pathology/ Histology Supervisor 6. Ny City - Histotech 7. NV - Histotech 3rd shift 8. GA - Histotech (all shifts) 9. OK - Histotech/ Histology Supervisor 10. NY City - Cytotech 11. Long Island, NY - Cytotech 12. PA - Cytology Supervisor 13. CT - Cytotech 14. Hematopathology and Flow Cytometry Technologist If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Happy 2010! Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From MSHERWOOD <@t> PARTNERS.ORG Tue Jan 5 09:51:21 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Jan 5 09:51:27 2010 Subject: [Histonet] Superfrost plus gold slides Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23EE2@PHSXMB30.partners.org> We are staining 5um FFPE sections of laser-treated human cartilage and are experiencing sections falling off the slides and, if not, then wrinkling. (We even had this problem with untreated cartilage, but it is worse with laser-treated). I know this topic has been discussed before and went to the Histonet archives, but did not get a clear answer to my query. Most of the discussion re: superfrost plus gold slides involved frozen sections, but I did not get a clear answer re: paraffin-embedded sections. We are currently using superfrost plus slides. I have been looking in the literature re: coated slides, but it is confusing. What do most histonetters recommend? (i.e. poly-l-lysine, gelatin, or silane coated Slides). Do you recommend superfrost plus gold slides? I would appreciate any advice. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From MSHERWOOD <@t> PARTNERS.ORG Tue Jan 5 10:11:01 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Jan 5 10:11:06 2010 Subject: [Histonet] Leica RM2255 motorized microtome Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23EE3@PHSXMB30.partners.org> To all: We are looking to purchase a used Leica RM2255 microtome. We are especially interested in any labs that looking to sell one for a good price, but we are not excluding any companies that deal with refurbished units. (We missed out on a recent RM2155 unit that was sold by a lab in Vermont.) If you are a lab/company and have such an instrument, please contact me by email (off-line). Do not call and leave a voice mail. If you are a company, please send me via email, a quote for the instrument. Thank you. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From ruebenjcarter <@t> gmail.com Tue Jan 5 11:06:55 2010 From: ruebenjcarter <@t> gmail.com (R C) Date: Tue Jan 5 11:06:59 2010 Subject: [Histonet] 2x3 protocol for monkey brain Message-ID: <2a926e3f1001050906w39c5ee22gbbdbd61b5fdb8bfa@mail.gmail.com> I want to thank all that have repsonded to my advice inquiry. Cheers! Ruben Carter From Dorothy.L.Webb <@t> HealthPartners.Com Tue Jan 5 11:23:45 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Jan 5 11:23:51 2010 Subject: [Histonet] Special stains Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C56FA39861@HPEMX3.HealthPartners.int> We are switching automated special stain equipment to a different vendor and am wondering if we have to validate all of the special stains we do. I am from the "old school" and the validation process, other than IHC, is not my forte!! Any advice would be appreciated, so, thanks ahead of time!! Dorothy Webb, Ht (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From Kim.Donadio <@t> bhcpns.org Tue Jan 5 11:28:29 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Jan 5 11:28:44 2010 Subject: [Histonet] Special stains In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43C56FA39861@HPEMX3.HealthPartners.int> Message-ID: Yes Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Webb, Dorothy L" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/05/2010 11:23 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] Special stains We are switching automated special stain equipment to a different vendor and am wondering if we have to validate all of the special stains we do. I am from the "old school" and the validation process, other than IHC, is not my forte!! Any advice would be appreciated, so, thanks ahead of time!! Dorothy Webb, Ht (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From lyork <@t> kwbpathology.com Tue Jan 5 10:30:12 2010 From: lyork <@t> kwbpathology.com (LYork) Date: Tue Jan 5 11:30:27 2010 Subject: [Histonet] Job Posting Panama City Florida Message-ID: <000b01ca8e24$5dfda030$8e01a8c0@KWBPA.local> Experienced Histology Technician needed for new lab in dermatology practice. Responsibilities include grossing, processing, embedding, cutting, staining, and cover-slipping of skin biopsies. HT(ASCP) or HTL(ASCP) certification preferred. Lab is located on the coast of Florida in Panama City. Position includes excellent compensation and benefits. Drug and criminal background screening are required. For more information contact Hannah Ross at: hannahstout.ross@gmail.com or 850-233-3376. ************************************************************************************ This footnote confirms that this email message has been scanned by PineApp Mail-SeCure for the presence of malicious code, vandals & computer viruses. ************************************************************************************ From rjbuesa <@t> yahoo.com Tue Jan 5 11:38:29 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 5 11:38:33 2010 Subject: [Histonet] Special stains In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43C56FA39861@HPEMX3.HealthPartners.int> Message-ID: <753451.33066.qm@web65709.mail.ac4.yahoo.com> Dorothy: I would NOT do it. If you do not change the procedure it does not really matter which auto stainer you use. Your "validation" is the reaction of your control that should react as before. As a matter of fact, I would be more inclined to made a validation if you change your control rather than if you change the auto stainer. If, for example, you are doing IHC and your dilution has been "fine tuned" for a given tissue control and you change the tissue, you will need to "fine tune" the dilution to have the same reaction?strength of the epitope in the new control, but you will not have to change your procedure. That is why I always used IHC internal controls, and by using "normal strength epitopes" I don't have to keep validating. That is also why I always shied away from using pathological cases to use as epitope controls. So, I would not advise you to go through validation because of changing the auto stainer, as long as the procedure is the same. Ren? J. --- On Tue, 1/5/10, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Special stains To: "'histonet@lists.utsouthwestern.edu'" Date: Tuesday, January 5, 2010, 12:23 PM We are switching automated special stain equipment to a different vendor and am wondering if we have to validate all of the special stains we do.? I am from the "old school" and the validation process, other than IHC, is not my forte!!? Any advice would be appreciated, so, thanks ahead of time!! Dorothy Webb, Ht (ASCP) Regions Histology Technical Supervisor 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Jan 5 12:21:04 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Jan 5 12:21:09 2010 Subject: [Histonet] Special stains In-Reply-To: <753451.33066.qm@web65709.mail.ac4.yahoo.com> Message-ID: Rene I'm sorry but I'm going to have to disagree with you on this one. But I'm coming from a GLP lab. Different stainers stain differently. We have two different brands of IHC stainers and even with most of the same reagents (rinse buffers are different) the staining comes out different on each one with basically the same protocol. Plus in a GLP lab we have to validate the stainer itself. All of our equipment has been validated and when we moved to our new location we had to re validate everything again. Our validation protocols are about 50 to 70 pages long for each of the IHC stainers we have, along with validation of the PT links, with data tracers for temp, etc. Not a simple task, but it needs to be done. Once I have a GLP IHC protocol in place I can't change any equipment or reagents without revalidating the procedure. It might be different in clinical but in the GLP world I would revalidate the special stains also. It should not be that difficult to do, just stain a couple control slides and determine if the staining is correct and document it. A one page sheet could be created for each stain (document vendor, lot numbers and exp date of reagents, serial number of stainer, date performed, person performing the staining, what control slides were used and then the slides should be reviewed by the tech that stained it and then the pathologist, record date reviewed with initials, comments, etc. I don't think much more would be needed for specials. IHC would be different, more slides for validation, additional paperwork is required. There is a paper out there on recommendations on how to validate IHC staining. I have a pdf of it if anyone is interested. That's just my two cents, but I have the tendency to do more than to do less, since the last thing I need is a 483 from the FDA when they come and visit. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 05, 2010 10:38 AM To: 'histonet@lists.utsouthwestern.edu'; Dorothy LWebb Subject: Re: [Histonet] Special stains Dorothy: I would NOT do it. If you do not change the procedure it does not really matter which auto stainer you use. Your "validation" is the reaction of your control that should react as before. As a matter of fact, I would be more inclined to made a validation if you change your control rather than if you change the auto stainer. If, for example, you are doing IHC and your dilution has been "fine tuned" for a given tissue control and you change the tissue, you will need to "fine tune" the dilution to have the same reaction?strength of the epitope in the new control, but you will not have to change your procedure. That is why I always used IHC internal controls, and by using "normal strength epitopes" I don't have to keep validating. That is also why I always shied away from using pathological cases to use as epitope controls. So, I would not advise you to go through validation because of changing the auto stainer, as long as the procedure is the same. Ren? J. --- On Tue, 1/5/10, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Special stains To: "'histonet@lists.utsouthwestern.edu'" Date: Tuesday, January 5, 2010, 12:23 PM We are switching automated special stain equipment to a different vendor and am wondering if we have to validate all of the special stains we do.? I am from the "old school" and the validation process, other than IHC, is not my forte!!? Any advice would be appreciated, so, thanks ahead of time!! Dorothy Webb, Ht (ASCP) Regions Histology Technical Supervisor 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> gradymem.org Tue Jan 5 12:23:44 2010 From: histology <@t> gradymem.org (Histology Dept) Date: Tue Jan 5 12:24:53 2010 Subject: [Histonet] CLIA Inspection Message-ID: We have been informed that we will have a CLIA inspection in two weeks. Any tips/pointers? For the volume of tests done does GYN Pathology and Non-GYN Pathology refer to cytologies only? Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/779-2258 histology@gradymem.org From Kim.Donadio <@t> bhcpns.org Tue Jan 5 12:28:24 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Jan 5 12:28:48 2010 Subject: [Histonet] Special stains In-Reply-To: <753451.33066.qm@web65709.mail.ac4.yahoo.com> Message-ID: In the end it is still a new instrument. It should be validated for all the stains you do on it. This would seem like best practice to me. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 01/05/2010 11:38 AM To "'histonet@lists.utsouthwestern.edu'" , Dorothy LWebb cc Subject Re: [Histonet] Special stains Dorothy: I would NOT do it. If you do not change the procedure it does not really matter which auto stainer you use. Your "validation" is the reaction of your control that should react as before. As a matter of fact, I would be more inclined to made a validation if you change your control rather than if you change the auto stainer. If, for example, you are doing IHC and your dilution has been "fine tuned" for a given tissue control and you change the tissue, you will need to "fine tune" the dilution to have the same reaction strength of the epitope in the new control, but you will not have to change your procedure. That is why I always used IHC internal controls, and by using "normal strength epitopes" I don't have to keep validating. That is also why I always shied away from using pathological cases to use as epitope controls. So, I would not advise you to go through validation because of changing the auto stainer, as long as the procedure is the same. Ren? J. --- On Tue, 1/5/10, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Special stains To: "'histonet@lists.utsouthwestern.edu'" Date: Tuesday, January 5, 2010, 12:23 PM We are switching automated special stain equipment to a different vendor and am wondering if we have to validate all of the special stains we do. I am from the "old school" and the validation process, other than IHC, is not my forte!! Any advice would be appreciated, so, thanks ahead of time!! Dorothy Webb, Ht (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From christina.thurby <@t> bms.com Tue Jan 5 12:32:57 2010 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Tue Jan 5 12:33:05 2010 Subject: [Histonet] RE: Superfrost plus gold slides (Sherwood, Margaret ) In-Reply-To: <87404f58-276b-479a-a81d-dff3c0371cfa@ushpwbmsmhp001.one.ads.bms.com> References: <87404f58-276b-479a-a81d-dff3c0371cfa@ushpwbmsmhp001.one.ads.bms.com> Message-ID: Peggy, In response to your question, the superfrost plus gold slides do work really well with FFPE tissues. Under a separate e-mail I'll send you a paper that our laboratory completed using this type of slide (superfrost plus gold vs. superfrost plus) for immuno work involving the brain and gallbladder using animal tissue. Christina Thurby Bristol Myers Squibb phone 812-307-2093 >Message: 13 >Date: Tue, 5 Jan 2010 10:51:21 -0500 >From: "Sherwood, Margaret " >Subject: Re: [Histonet] Superfrost plus gold slides >To: >We are staining 5um FFPE sections of laser-treated human cartilage and are >experiencing sections falling off the slides and, if not, then wrinkling. (We >even had this problem with untreated cartilage, but it is worse with >laser-treated). > >I know this topic has been discussed before and went to the Histonet archives, >but did not get a clear answer to my query. Most of the discussion re: >superfrost plus gold slides involved frozen sections, but I did not get a clear >answer re: paraffin-embedded sections. > >We are currently using superfrost plus slides. I have been looking in the >literature re: coated slides, but it is confusing. What do most histonetters >recommend? (i.e. poly-l-lysine, gelatin, or silane coated >Slides). Do you recommend superfrost plus gold slides? > >I would appreciate any advice. > >Thanks! >Peggy > > >Peggy Sherwood >Lab Associate, Photopathology >Wellman Center for Photomedicine (EDW 214) >Massachusetts General Hospital >55 Fruit Street >Boston, Massachusetts 02114-2696 >617-724-4839 (voice mail) >617-726-6983 (lab) >617-726-1206 (fax) >msherwood@partners.org This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From KGroeger <@t> USLABS.net Tue Jan 5 13:33:35 2010 From: KGroeger <@t> USLABS.net (Karin Groeger) Date: Tue Jan 5 14:05:18 2010 Subject: [Histonet] Special stains In-Reply-To: References: <753451.33066.qm@web65709.mail.ac4.yahoo.com> Message-ID: I agree, we are validating some new instruments now and have found a lot of variables. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Tuesday, January 05, 2010 10:28 AM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; histonet-bounces@lists.utsouthwestern.edu; Dorothy LWebb Subject: Re: [Histonet] Special stains In the end it is still a new instrument. It should be validated for all the stains you do on it. This would seem like best practice to me. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 01/05/2010 11:38 AM To "'histonet@lists.utsouthwestern.edu'" , Dorothy LWebb cc Subject Re: [Histonet] Special stains Dorothy: I would NOT do it. If you do not change the procedure it does not really matter which auto stainer you use. Your "validation" is the reaction of your control that should react as before. As a matter of fact, I would be more inclined to made a validation if you change your control rather than if you change the auto stainer. If, for example, you are doing IHC and your dilution has been "fine tuned" for a given tissue control and you change the tissue, you will need to "fine tune" the dilution to have the same reaction strength of the epitope in the new control, but you will not have to change your procedure. That is why I always used IHC internal controls, and by using "normal strength epitopes" I don't have to keep validating. That is also why I always shied away from using pathological cases to use as epitope controls. So, I would not advise you to go through validation because of changing the auto stainer, as long as the procedure is the same. Ren? J. --- On Tue, 1/5/10, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Special stains To: "'histonet@lists.utsouthwestern.edu'" Date: Tuesday, January 5, 2010, 12:23 PM We are switching automated special stain equipment to a different vendor and am wondering if we have to validate all of the special stains we do. I am from the "old school" and the validation process, other than IHC, is not my forte!! Any advice would be appreciated, so, thanks ahead of time!! Dorothy Webb, Ht (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. From Janice.Mahoney <@t> alegent.org Tue Jan 5 14:28:17 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Jan 5 14:28:28 2010 Subject: [Histonet] Special stains In-Reply-To: References: <65365F35C0F2EF4D846EC3CA73E49C43C56FA39861@HPEMX3.HealthPartners.int> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A5553@EXCHMBC2.ad.ah.local> Yes Dorothy, You should validate and document that each stain (control) reacted as expected as well as the instrument functionality. I would have a Pathologist initial it as well. Remember to have your instrument training documentation for your techs as well. Good luck, Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Tuesday, January 05, 2010 11:28 AM To: Webb, Dorothy L Cc: 'histonet@lists.utsouthwestern.edu'; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Special stains Subject [Histonet] Special stains We are switching automated special stain equipment to a different vendor and am wondering if we have to validate all of the special stains we do. I am from the "old school" and the validation process, other than IHC, is not my forte!! Any advice would be appreciated, so, thanks ahead of time!! Dorothy Webb, Ht (ASCP) Regions Histology Technical Supervisor 651-254-2962 Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. 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From jtaylor <@t> meriter.com Tue Jan 5 15:02:57 2010 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Tue Jan 5 15:33:30 2010 Subject: [Histonet] CISH - HER2 Message-ID: <466B666475DE6547BBB0641E540A4BB506451DCCD1@EXVS1.meriter.com> The pathologists that I work for are requesting that our lab look into starting up CISH mainly for HER2. What company are labs purchasing their CISH HER2 kits from? Does anyone use Dako's DuoCISH kit along with their HER2 FISH pharmDx kit? We currently run the old Dako Autostainers, but are in the market for purchasing a new IHC stainer as well. Thanks for your help! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From pruegg <@t> ihctech.net Tue Jan 5 15:38:36 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jan 5 16:24:27 2010 Subject: [Histonet] RE: [IHCRG] CISH - HER2 In-Reply-To: <466B666475DE6547BBB0641E540A4BB506451DCCD1@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB506451DCCD1@EXVS1.meriter.com> Message-ID: Jean, I am in the process of optimizing some CISH on the Leica Bond instrument, not Her2 but our own custom probes, it is nice that you can do the hybridization on the instrument, we are using the Leica hybridization buffer developed to use with anyone's probes not just the ones they are marketing (just Kappa and Lambda, EBer I believe for now, I think they are working on Hpv), it is very promising. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Taylor, Jean Sent: Tuesday, January 05, 2010 2:03 PM To: 'histonet@lists.utsouthwestern.edu'; 'ihcrg@googlegroups.com' Subject: [IHCRG] CISH - HER2 The pathologists that I work for are requesting that our lab look into starting up CISH mainly for HER2. What company are labs purchasing their CISH HER2 kits from? Does anyone use Dako's DuoCISH kit along with their HER2 FISH pharmDx kit? We currently run the old Dako Autostainers, but are in the market for purchasing a new IHC stainer as well. Thanks for your help! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From wdesalvo.cac <@t> hotmail.com Tue Jan 5 20:11:16 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Jan 5 20:11:22 2010 Subject: [Histonet] Special stains In-Reply-To: References: <753451.33066.qm@web65709.mail.ac4.yahoo.com>, , Message-ID: I agree with the majority that you MUST do a validation of the instrument before you run any patient diagnostic samples. Any time you make a change in instrumentation, reagents, procedure or protocol you should run at least a quick validation study to compare results of the new variable(s) to your control method (current) and it must be reviewed and approved by the medical director of your lab. The numbr of samples is always the question and I suggest you investigate whether or not your institution has a an expectation for sample size. The Clinicl lab will be a good place to get information on procedure and process for validation. That said and for an automated stainer in the Histology lab (Dako or Ventana), I suggest a minimum of three samples on separate runs for each special stain you will perform. If all three are comarable and correlate to the contol method, you can have confidence that you can make the switch and start turnung out patient samples. If you do not create comparable results for the first three, then consult w/ your pathologist as to how many more samples he will feel comfortable reviewing to confirm the performance. You should always stive to produce >95% concordance. I also suggest that you use the "blind study" technique when presenting to the reviewing pathologist. You do not want perception or predjudice to skew your review results. Of course, document all that you do and create a validation folder to house the documents for easy review. I believe setting up a validation process/protocol for your lab will create more confidence in the instrument, the reagents, the technicians performing the work and you. I have found it is easier to be proactive rather than reactive in producing quality results. Good luck with moving to the automated special stainer. I have used the DAKO for 4+ years and find it will produce a quality stain consistantly. William DeSalvo, B.S., HTL(ASCP) Chairperson, NSH QCC > Date: Tue, 5 Jan 2010 11:33:35 -0800 > From: KGroeger@USLABS.net > To: Kim.Donadio@bhcpns.org; rjbuesa@yahoo.com > Subject: RE: [Histonet] Special stains > CC: histonet@lists.utsouthwestern.edu; Dorothy.L.Webb@HealthPartners.Com; histonet-bounces@lists.utsouthwestern.edu > > I agree, we are validating some new instruments now and have found a lot of variables. > > Karin Groeger > > Histology Supervisor > > US LABS, Irvine,CA > > 949-450-0145 ext. 649 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org > Sent: Tuesday, January 05, 2010 10:28 AM > To: Rene J Buesa > Cc: 'histonet@lists.utsouthwestern.edu'; histonet-bounces@lists.utsouthwestern.edu; Dorothy LWebb > Subject: Re: [Histonet] Special stains > > In the end it is still a new instrument. It should be validated for all > the stains you do on it. This would seem like best practice to me. > > > > Kim Donadio > Pathology Supervisor > Baptist Hospital > 1000 W Moreno St. > Pensacola FL 32501 > Phone (850) 469-7718 > Fax (850) 434-4996 > > > > Rene J Buesa > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/05/2010 11:38 AM > > To > "'histonet@lists.utsouthwestern.edu'" , > Dorothy LWebb > cc > > Subject > Re: [Histonet] Special stains > > > > > > > Dorothy: > I would NOT do it. > If you do not change the procedure it does not really matter which auto > stainer you use. Your "validation" is the reaction of your control that > should react as before. > As a matter of fact, I would be more inclined to made a validation if you > change your control rather than if you change the auto stainer. > If, for example, you are doing IHC and your dilution has been "fine tuned" > for a given tissue control and you change the tissue, you will need to > "fine tune" the dilution to have the same reaction strength of the epitope > in the new control, but you will not have to change your procedure. That > is why I always used IHC internal controls, and by using "normal strength > epitopes" I don't have to keep validating. > That is also why I always shied away from using pathological cases to use > as epitope controls. > So, I would not advise you to go through validation because of changing > the auto stainer, as long as the procedure is the same. > Ren? J. > > --- On Tue, 1/5/10, Webb, Dorothy L > wrote: > > > From: Webb, Dorothy L > Subject: [Histonet] Special stains > To: "'histonet@lists.utsouthwestern.edu'" > > Date: Tuesday, January 5, 2010, 12:23 PM > > > We are switching automated special stain equipment to a different vendor > and am wondering if we have to validate all of the special stains we do. > I am from the "old school" and the validation process, other than IHC, is > not my forte!! Any advice would be appreciated, so, thanks ahead of > time!! > > Dorothy Webb, Ht (ASCP) > Regions Histology Technical Supervisor > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----------------------------------------- > All electronic data transmissions originating from or sent to > Baptist Health Care Corporation (BHC) are subject to monitoring. > This message along with any attached data, are the confidential and > proprietary communications of BHC and are intended to be received > only by the individual or individuals to whom the message has been > addressed. If the reader of this message is not the intended > recipient, please take notice that any use, copying, printing, > forwarding or distribution of this message, in any form, is > strictly prohibited and may violate State or Federal Law. If you > have received this transmission in error, please delete or destroy > all copies of this message. For questions, contact the BHC Privacy > Officer at (850) 434-4472. Rev.10/07. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/171222986/direct/01/ From LilianaB <@t> asaf.health.gov.il Wed Jan 6 01:18:52 2010 From: LilianaB <@t> asaf.health.gov.il (LilianaB@asaf.health.gov.il) Date: Wed Jan 6 01:19:53 2010 Subject: [Histonet] Superfrost plus gold slides Message-ID: <054452CCC076BE4DA21E46AE95E32EC304B496@mail2.asaf.health.gov.il> Dear Peggy, We tried the Gold Super Plus for FISH and the paraffin sections fell from the slide. We are using the Super Frost Ultra from Menzel, but when the tissue is thick they can wash out from the slide. So try to cut the tissue 3 or 4 microns. I hope this help you. Kind regards, Liliana Immunohistochemistry Supervisor Dept of Pathology Asaf Harofeh Medical Center Israel From LilianaB <@t> asaf.health.gov.il Wed Jan 6 01:32:54 2010 From: LilianaB <@t> asaf.health.gov.il (LilianaB@asaf.health.gov.il) Date: Wed Jan 6 01:33:54 2010 Subject: [Histonet] CISH - HER2 Message-ID: <054452CCC076BE4DA21E46AE95E32EC304B497@mail2.asaf.health.gov.il> Dear Jean, If you don't need FDA approved technique you can try the new SISH Her-2 staining on Ventana Benchmark XT. It works great and is fully automatic. If you need FDA, you can use Pathvysion Her-2 from Vysis, but is a fluorescence technique and is fully manual. Good luck, Liliana Habler Immunohistochemistry Manager Dept. of Pathology Asaf Harofeh Medical Center Israel From W.E.J.Hoekert <@t> olvg.nl Wed Jan 6 03:02:15 2010 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Wed Jan 6 03:06:45 2010 Subject: [Histonet] CISH - HER2 References: <466B666475DE6547BBB0641E540A4BB506451DCCD1@EXVS1.meriter.com> Message-ID: <1190CB05C44B13409483514729C2FC360C0A6E@PAIT42.olvg.nl> We are using Spot Light her2 Cish from invitrogen (manual, FDA approved). Willem Hoekert Pathology OLVG, Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Taylor, Jean Verzonden: di 5-1-2010 22:02 Aan: 'histonet@lists.utsouthwestern.edu'; 'ihcrg@googlegroups.com' Onderwerp: [Histonet] CISH - HER2 The pathologists that I work for are requesting that our lab look into starting up CISH mainly for HER2. What company are labs purchasing their CISH HER2 kits from? Does anyone use Dako's DuoCISH kit along with their HER2 FISH pharmDx kit? We currently run the old Dako Autostainers, but are in the market for purchasing a new IHC stainer as well. Thanks for your help! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From Robert.Cordero <@t> comphealth.com Wed Jan 6 08:52:09 2010 From: Robert.Cordero <@t> comphealth.com (Robert.Cordero@comphealth.com) Date: Wed Jan 6 08:52:39 2010 Subject: [Histonet] Referrals Needed for Staff Histology Openings in Illinois, Kansas and Oklahoma In-Reply-To: <858249121001041053i250a12b3p390f45a27720a2fc@mail.gmail.com> Message-ID: Hi, all! I wanted to check in and see if anyone was possibly interested or knew of someone who would be interested in a few day shift opportunities we had posted in Illinois, Kansas and Oklahoma? All three opportunities are offering relocation assistance, full benefits and competitive salaries. The Oklahoma opportunity is wanting someone who would be able to move into a Leadership role in the near future. The others would like an HT/HTL who has at least one year of experience, but might consider a sharp new grad. Please contact me directly if you have any questions. Thank you! Regards, Rob Cordero Imaging and Laboratory Sciences Consultant Permanent Placement Division CompHealth Associates, Inc. Phone: 866.782.9029 x 5866 Direct: 954.343.5866 Fax: 800.420.2329 Please note: "This is a commercial email from CompHealth Associates, Inc. If you do not want to receive future emails from CompHealth Associates, Inc., please reply to the sender of this email and ask to be removed from our list." From JSilverman <@t> NSHS.edu Wed Jan 6 08:57:17 2010 From: JSilverman <@t> NSHS.edu (Silverman, Jeffrey) Date: Wed Jan 6 08:57:29 2010 Subject: [Histonet] Biogenex i6000 or ???? Message-ID: <9CC9B752A161C343A60908591464DAA70202B585@EXCHVS02.nslijhs.net> Hello Histonetters, It's been a long long time since I've subscribed, and I'm back since I need help. We currently run a Dako Artisan for our limited IHC menu and tinctorial special stains. I've been informed that Dako will stop supporting IHC on the Artisan in about 18 months. Our machine is very old and is beginning to crap out on us. They want to give us an Artisan Classic replacement on a reagent rental basis but the loss of IHC support soon would be a major impediment to our services. The Biogenex i6000 has caught my eye and I'd like to hear from any users out their with their experiences good or bad. We have a small workload of IHC and specials and space is non-existent so we cannot use a dedicated IHC machine, we need an instrument that does both and will not result in our discarding thousands of dollars of expired unused reagents due to our small workload. Users of any other similar instruments are encouraged to reply as well. All the best in 2010 to all you guys and gals out there. Jeffrey S. Silverman HT HTL QIHC (ASCP) Pathologists' Assistant Southside Hospital Bay Shore, New York USA From joost.bruijntjes <@t> tno.nl Wed Jan 6 09:44:05 2010 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Wed Jan 6 09:44:13 2010 Subject: [Histonet] =?iso-8859-7?q?=E1-2_microglobulin_rat?= Message-ID: <8865601DD17A554CB489C17FFD8A51B203154300@MAIL04.tsn.tno.nl> Hi All Is anyone of you aware of a supplier of an antibody directed against ?-2 microglobulin applicable on rat kidneys? I have found one, but is should be working in Elisa en Western blotting only, and I need it for immunohistochemistry. Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From Ronald.Houston <@t> nationwidechildrens.org Wed Jan 6 10:35:53 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Jan 6 10:37:56 2010 Subject: [Histonet] Peloris - xylene free Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B645F10@chi2k3ms01.columbuschildrens.net> Is anyone using the Peloris tissue processor with isopropanol instead of alcohol/xylene? I am interested in your experiences. We are demoing the machine just now and have been very impressed with its results using traditional processing modules. However, when we switched over to isopropanol, the tissue is extremely brittle; not what I would expect having had extensive microwave processing experience. We have used the protocols suggested by Leica. Anyone doing anything dramatically different? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From liz <@t> premierlab.com Wed Jan 6 10:49:18 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jan 6 10:49:23 2010 Subject: [Histonet] a-2 microglobulin rat In-Reply-To: <8865601DD17A554CB489C17FFD8A51B203154300@MAIL04.tsn.tno.nl> Message-ID: We have done this before and the antibody we used came from SSI, Catalog Number: HYB 339-01. It worked fine on FFPE rat kidney samples. ilz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruijntjes, J.P. (Joost) Sent: Wednesday, January 06, 2010 8:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] a-2 microglobulin rat Hi All Is anyone of you aware of a supplier of an antibody directed against ?-2 microglobulin applicable on rat kidneys? I have found one, but is should be working in Elisa en Western blotting only, and I need it for immunohistochemistry. Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From liz <@t> premierlab.com Wed Jan 6 12:30:22 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jan 6 12:30:27 2010 Subject: [Histonet] IHC recommendations paper - FYI Message-ID: A lot of people have asked me for the paper that I mentioned in my e-mail yesterday. I think I found it on the CAP website, but here is the papers title and authors. It's a review article. Its helped me a lot with IHC validation. VM114 Standardization and Troubleshooting Immunohistochemistry for Pathologists Neal S. Goldstein, MD October 2, 2007 8:30 AM - Noon Recommendations for Improved Standardization of Immunohistochemistry Neal S. Goldstein, MD, Stephen M. Hewitt, MD, PhD, Clive R. Taylor, MD, DPhil, Hadi Yaziji, MD, David G. Hicks, MD, and Members of Ad-Hoc Committee On Immunohistochemistry Standardization Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From Soo-Jin.Cho <@t> ucsfmedctr.org Wed Jan 6 12:45:53 2010 From: Soo-Jin.Cho <@t> ucsfmedctr.org (Cho, Soo-Jin) Date: Wed Jan 6 12:46:08 2010 Subject: [Histonet] Policy on Floaters Message-ID: <622D25C2D399AC40A7CDCF12351B5151FB8CA811@exmbmcb12.ucsfmedicalcenter.org> Hello, I'm a resident at UCSF currently working on a QA/QI project regarding floaters, with the ultimate goal of formulating a departmental policy regarding floaters. Despite extensive searching on the internet and the Histonet archives, I have not found any concrete examples of policies at other institutions and was hoping someone could help me out in this regard. Thank you in advance for your help. Most sincerely, Soo-Jin Cho Anatomic Pathology University of California, San Francisco From litepath2000 <@t> yahoo.com Wed Jan 6 13:33:57 2010 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Wed Jan 6 13:34:02 2010 Subject: [Histonet] NYS Licensing Examination Update Message-ID: <969352.91010.qm@web58803.mail.re1.yahoo.com> Hi Everyone We have excellent news......!! we?have just received this update from the New York State Office of Professions regarding the examination procedures and eligibility for licensing in NYS, Please see below.? Since this is "hot off the press" information, I am sure there will be questions but please be patient as the procedures are formally written and posted on the website (http://www.op.nysed.gov/).??For those of you who want to be updated regularly on whats going on in NY, please join the New York State Histotechnological Society message board at:?http://tech.groups.yahoo.com/group/NYSHS1972/?If you have questions regarding licensing in NYS please feel free to contact us by email. Regards Luis Chiriboga & Amy Farnan,? NYSHS Legislative Committee ? ------- Within the past few weeks, our contract with ASCP for our candidates to use the ASCP exam for licensure as a certified histological technician has been approved by the State Comptroller and we have begun to implement the use of this examination.? Applicants for licensure as certified histological technician will use the same examination, during the five year period for the contract, as is used by ASCP for their certification purposes.? But, these are separate procedures.? Applicants for licensure pay the fee for the exam that is established by our contract, have scores reported directly to us, and have the right to take the examination as often as needed.? The same persons may also want a certificate from ASCP.? If so, they will have to apply to ASCP for this certificate, pay their fee, meet their requirements, and take the examination according to their specifications.? These are totally separate processes.? We simply contract for the examination and do not endorse the ASCP certification.? For persons who have applied for this license recently, we may have issued a limited permit and they will have to take the examination prior to licensure during the one year period that the permit is valid.? There are also some persons who have received a limited license as a certified histological technician.? These persons will have to meet not only the examination, but the full education requirements.? For those persons who hold a limited permit and did take and pass the examination after September 1, 2001, their examination results may be used for licensure purposes and they will not have to retake the examination.? Anyone who took the examination prior to September 1, 2001 will have to take the examination now for it to be used for licensure.? We have received a number of score reports from ASCP on applicants who now hold a limited permit, and we will now be processing the applications for licensure, as long as all requirements are met. Finally, a note on another topic.? There are many persons who work as histological technicians who were originally licensed as certified clinical laboratory technicians and who may also be licensed as certified histological technicians.? The reregistration period for the license as a certified clinical laboratory technician may now be expiring.? These person have the choice of reregistering as a certified clinical laboratory technician or placing that registration in an "inactive status," (and they must inform us if that is their intention) or of maintaining both registrations, or of placing their registration, when it comes up, on an "inactive status."? Both licenses will continue unexpired for life unless removed by disciplinary action, but the person may only practice in the areas authorized under the scope of the profession in which they are licensed and registered.? The scope of both licenses authorize the practice of histological technician. Kathleen Dr. Kathleen M. Doyle Executive Secretary kdoyle2@mail.nysed.gov 518-474-3817, ext. 150 ------- Luis Chiriboga Ph.D. Assistant Professor of Pathology Vice President New York State Histotechnological Society NYSHS Website www.nyhisto.org NYSHS Message Board http://tech.groups.yahoo.com/group/NYSHS1972/ From rjbuesa <@t> yahoo.com Wed Jan 6 14:02:21 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 6 14:02:25 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: <622D25C2D399AC40A7CDCF12351B5151FB8CA811@exmbmcb12.ucsfmedicalcenter.org> Message-ID: <483213.60612.qm@web65709.mail.ac4.yahoo.com> It is difficult for you to find a "policy on floaters" because any lab will try to not get any floater. It could be said that 1 floater is 1 too many. The goal is "0" floaters. Ren? J. --- On Wed, 1/6/10, Cho, Soo-Jin wrote: From: Cho, Soo-Jin Subject: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, January 6, 2010, 1:45 PM Hello, I'm a resident at UCSF currently working on a QA/QI project regarding floaters, with the ultimate goal of formulating a departmental policy regarding floaters.? Despite extensive searching on the internet and the Histonet archives, I have not found any concrete examples of policies at other institutions and was hoping someone could help me out in this regard.? Thank you in advance for your help. Most sincerely, Soo-Jin Cho Anatomic Pathology University of California, San Francisco _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Wed Jan 6 14:12:58 2010 From: bill501 <@t> mindspring.com (Bill) Date: Wed Jan 6 14:13:03 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: <483213.60612.qm@web65709.mail.ac4.yahoo.com> References: <483213.60612.qm@web65709.mail.ac4.yahoo.com> Message-ID: At 12:02 PM -0800 1/6/10, Rene J Buesa wrote: >It is difficult for you to find a "policy on floaters" because any lab will try to not get any floater. >It could be said that 1 floater is 1 too many. The goal is "0" floaters. Since no one is perfect and floaters WILL happen at every lab, what do you do when one raises its ugly head? Bill B From lblazek <@t> digestivespecialists.com Wed Jan 6 14:17:25 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jan 6 14:18:30 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: References: <483213.60612.qm@web65709.mail.ac4.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390E92C0DA14@IBMB7Exchange.digestivespecialists.com> Document it and see it there is a trend. Linda B -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Wednesday, January 06, 2010 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Policy on Floaters At 12:02 PM -0800 1/6/10, Rene J Buesa wrote: >It is difficult for you to find a "policy on floaters" because any lab will try to not get any floater. >It could be said that 1 floater is 1 too many. The goal is "0" floaters. Since no one is perfect and floaters WILL happen at every lab, what do you do when one raises its ugly head? Bill B _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Jan 6 14:21:49 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jan 6 14:21:53 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: <622D25C2D399AC40A7CDCF12351B5151FB8CA811@exmbmcb12.ucsfmedicalcenter.org> References: <622D25C2D399AC40A7CDCF12351B5151FB8CA811@exmbmcb12.ucsfmedicalcenter.org> Message-ID: <5b6eb13e1001061221h32020c6fhf38e09d513f6d86f@mail.gmail.com> Your goal is not to have floaters. If you get one, your policy should set out to determine the cause of these incidents. You should track who did it (in a spreadsheet), where it happened (grossing, embedding, cutting...). Then you should have a meeting every so often with people from the lab and some pathologists where you go over all the incidents, brainstorm for corrective actions, and decide about what you can do differently. Make sure you have the techs invovled or it won't be very effective. Mark On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin wrote: > Hello, I'm a resident at UCSF currently working on a QA/QI project > regarding floaters, with the ultimate goal of formulating a departmental > policy regarding floaters. Despite extensive searching on the internet and > the Histonet archives, I have not found any concrete examples of policies at > other institutions and was hoping someone could help me out in this regard. > Thank you in advance for your help. > > Most sincerely, > Soo-Jin Cho > Anatomic Pathology > University of California, San Francisco > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jcampbell <@t> vdxpathology.com Wed Jan 6 15:01:19 2010 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Wed Jan 6 15:01:23 2010 Subject: [Histonet] special stains Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF82FA87B@VDXSERVER01.vdxpathology.local> I couldn't agree with you more, Liz! I am from a GLP lab as well and we have to undergo validation for every single new piece of equipment we acquire otherwise we are not considered GLP-compliant. Who is to say that your new stainer is going to stain slides in the same manner as your old stainer?--which is exactly why you have to validate your equipment and ensure that it is performing what the user has programmed it to perform and giving you accurate and consistent results. And we re-validate any time a change is made as well, whether we switch equipment or make a change to a staining protocol. We have special forms to document staining results and access to runtime logs on our stainer which allows us to ensure that the stainer carried out the correct protocol steps. Jen From tbraud <@t> holyredeemer.com Wed Jan 6 15:08:09 2010 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Jan 6 15:08:19 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: <483213.60612.qm@web65709.mail.ac4.yahoo.com> Message-ID: Ok. I have to wade in. We actually have a policy on floaters. Our policy calls for the pathologist to circle the floater on the slide and mark it as a floater with a cell marking pen. That way the "floater" is identified forever on the permanent slide. He/she will notify the Supv or lead tech to investigate. If the floater is located within the block, then we dig it out of the block. We record the slide I.D., floater, and resolution on the daily stain qc sheet. Unless we have an ongoing problem, we don't track these any further but the data is there to trend, and if necessary, include in any QC, CQI, QA program. Hope this helps, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax From: Cho, Soo-Jin Subject: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, January 6, 2010, 1:45 PM Hello, I'm a resident at UCSF currently working on a QA/QI project regarding floaters, with the ultimate goal of formulating a departmental policy regarding floaters.? Despite extensive searching on the internet and the Histonet archives, I have not found any concrete examples of policies at other institutions and was hoping someone could help me out in this regard.? Thank you in advance for your help. Most sincerely, Soo-Jin Cho Anatomic Pathology University of California, San Francisco _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Wed Jan 6 15:54:36 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 6 15:54:39 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: Message-ID: <449264.32138.qm@web65716.mail.ac4.yahoo.com> You document it in the QA file and prepare a follow up in the PI program. Ren? J. --- On Wed, 1/6/10, Bill wrote: From: Bill Subject: Re: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, January 6, 2010, 3:12 PM At 12:02 PM -0800 1/6/10, Rene J Buesa wrote: >It is difficult for you to find a "policy on floaters" because any lab will try to not get any floater. >It could be said that 1 floater is 1 too many. The goal is "0" floaters. Since no one is perfect and floaters WILL happen at every lab, what do you do when one raises its ugly head? Bill B _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FUNKM <@t> mercyhealth.com Wed Jan 6 16:22:44 2010 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed Jan 6 16:22:58 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: <5b6eb13e1001061221h32020c6fhf38e09d513f6d86f@mail.gmail.com> References: <622D25C2D399AC40A7CDCF12351B5151FB8CA811@exmbmcb12.ucsfmedicalcenter.org> <5b6eb13e1001061221h32020c6fhf38e09d513f6d86f@mail.gmail.com> Message-ID: <4B44B8D4.9B87.00AC.0@mercyhealth.com> Floaters Yes, you are so right, for patient safety and your safety, policy is a must. Protects you and the patient. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> Mark Tarango 01/06/2010 2:21 PM >>> Your goal is not to have floaters. If you get one, your policy should set out to determine the cause of these incidents. You should track who did it (in a spreadsheet), where it happened (grossing, embedding, cutting...). Then you should have a meeting every so often with people from the lab and some pathologists where you go over all the incidents, brainstorm for corrective actions, and decide about what you can do differently. Make sure you have the techs invovled or it won't be very effective. Mark On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin wrote: > Hello, I'm a resident at UCSF currently working on a QA/QI project > regarding floaters, with the ultimate goal of formulating a departmental > policy regarding floaters. Despite extensive searching on the internet and > the Histonet archives, I have not found any concrete examples of policies at > other institutions and was hoping someone could help me out in this regard. > Thank you in advance for your help. > > Most sincerely, > Soo-Jin Cho > Anatomic Pathology > University of California, San Francisco > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ndevans <@t> stanford.edu Wed Jan 6 17:32:40 2010 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Wed Jan 6 17:32:43 2010 Subject: [histonet] Cytokeratin 17 Message-ID: <74C8CA0453A74B188D9C5FB3E7CDC247@DellDesktop2> Dear all, Can anyone suggest a decent, reliable antibody to mouse cytokeratin 17 (polyclonal or monoclonal is fine) I could buy? There are many on the market, but I want to be sure it works. Best wishes Nick From DKBoyd <@t> chs.net Thu Jan 7 07:18:07 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Jan 7 07:17:48 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: <4B44B8D4.9B87.00AC.0@mercyhealth.com> Message-ID: Lots of traffic on this one. Yes you definitely need a policy. The policy should define how you will "try" to eliminate floaters ie; clean water bath after each block, spray/wipe/change paper towels after each specimen while grossing etc. It also must include how it will be resolved. This covers everyone involved and is considered best practice. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Marcia Funk" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/06/2010 05:23 PM To "Mark Tarango" , "Soo-Jin Cho" cc "histonet@lists.utsouthwestern.edu" Subject Re: [Histonet] Policy on Floaters Floaters Yes, you are so right, for patient safety and your safety, policy is a must. Protects you and the patient. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> Mark Tarango 01/06/2010 2:21 PM >>> Your goal is not to have floaters. If you get one, your policy should set out to determine the cause of these incidents. You should track who did it (in a spreadsheet), where it happened (grossing, embedding, cutting...). Then you should have a meeting every so often with people from the lab and some pathologists where you go over all the incidents, brainstorm for corrective actions, and decide about what you can do differently. Make sure you have the techs invovled or it won't be very effective. Mark On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin wrote: > Hello, I'm a resident at UCSF currently working on a QA/QI project > regarding floaters, with the ultimate goal of formulating a departmental > policy regarding floaters. Despite extensive searching on the internet and > the Histonet archives, I have not found any concrete examples of policies at > other institutions and was hoping someone could help me out in this regard. > Thank you in advance for your help. > > Most sincerely, > Soo-Jin Cho > Anatomic Pathology > University of California, San Francisco > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From ree3 <@t> leicester.ac.uk Thu Jan 7 07:34:08 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Jan 7 07:34:37 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: References: <4B44B8D4.9B87.00AC.0@mercyhealth.com> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E0F3@EXC-MBX3.cfs.le.ac.uk> So, what exactly is a "floater", an extra bit of a previous section picked up with the next section in a dirty waterbath, or an obviously extra identifiable piece of tissue included in/picked up in error during grossing; bit of ambiguity in previous emails?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: 07 January 2010 13:18 To: Marcia Funk Cc: histonet@lists.utsouthwestern.edu; Soo-Jin Cho; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Policy on Floaters Lots of traffic on this one. Yes you definitely need a policy. The policy should define how you will "try" to eliminate floaters ie; clean water bath after each block, spray/wipe/change paper towels after each specimen while grossing etc. It also must include how it will be resolved. This covers everyone involved and is considered best practice. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Marcia Funk" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/06/2010 05:23 PM To "Mark Tarango" , "Soo-Jin Cho" cc "histonet@lists.utsouthwestern.edu" Subject Re: [Histonet] Policy on Floaters Floaters Yes, you are so right, for patient safety and your safety, policy is a must. Protects you and the patient. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> Mark Tarango 01/06/2010 2:21 PM >>> Your goal is not to have floaters. If you get one, your policy should set out to determine the cause of these incidents. You should track who did it (in a spreadsheet), where it happened (grossing, embedding, cutting...). Then you should have a meeting every so often with people from the lab and some pathologists where you go over all the incidents, brainstorm for corrective actions, and decide about what you can do differently. Make sure you have the techs invovled or it won't be very effective. Mark On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin wrote: > Hello, I'm a resident at UCSF currently working on a QA/QI project > regarding floaters, with the ultimate goal of formulating a departmental > policy regarding floaters. Despite extensive searching on the internet and > the Histonet archives, I have not found any concrete examples of policies at > other institutions and was hoping someone could help me out in this regard. > Thank you in advance for your help. > > Most sincerely, > Soo-Jin Cho > Anatomic Pathology > University of California, San Francisco > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Thu Jan 7 09:45:00 2010 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Jan 7 09:45:05 2010 Subject: [Histonet] osteopontin antibody Message-ID: <62A8156F8071C8439080D626DF8C33A6CEB2F0@wave-mail.7thwave.local> Can anyone recommend an osteopontin antibody that works in FFPE mouse tissue? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From MadaryJ <@t> MedImmune.com Thu Jan 7 10:38:27 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Thu Jan 7 10:38:36 2010 Subject: [Histonet] getting rid of surplus equipment-Source? Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A130102DEC4@MD1EV002.medimmune.com> Does anyone have a good source to get rid of equipment that is still good and maybe our lab can make a few bucks in the process? The last time I did this I was told the processor and stainer I gave away would be going to some charity and of course it did not I found out later. These items are off the books, maybe in need of a small repair. We have no room and the equipment is just been depreciated such that if we can get a credit or something for our lab as a trade in. Specifically we have an RMS microtome in perfect condition used for 6 months and put away. The other is a Leica Embedder that does everyting but dispense paraffin. We were using a separate paraffin dispenser and it was fine, but finally got another embedder. Anyway, vendors welcome. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From leiker <@t> buffalo.edu Thu Jan 7 11:01:42 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Jan 7 11:01:48 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E0F3@EXC-MBX3.cfs.le.ac.uk> References: <4B44B8D4.9B87.00AC.0@mercyhealth.com> <7722595275A4DD4FA225B92CDBF174A1E8C9F7E0F3@EXC-MBX3.cfs.le.ac.uk> Message-ID: I second that question! Seems like a lot of hassle (documenting, holding meetings) over a bit of waxy tissue left in a water bath (mind you, this is coming from a research mind-set where we don't mind these things so much). :-) --On Thursday, January 07, 2010 1:34 PM +0000 "Edwards, Richard E." wrote: > So, what exactly is a "floater", an extra bit of a previous section > picked up with the next section in a dirty waterbath, or an obviously > extra identifiable piece of tissue included in/picked up in error during > grossing; bit of ambiguity in previous emails?. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > DKBoyd@chs.net Sent: 07 January 2010 13:18 > To: Marcia Funk > Cc: histonet@lists.utsouthwestern.edu; Soo-Jin Cho; > histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Policy > on Floaters > > Lots of traffic on this one. Yes you definitely need a policy. The > policy should define how you will "try" to eliminate floaters ie; clean > water bath after each block, spray/wipe/change paper towels after each > specimen while grossing etc. It also must include how it will be > resolved. This covers everyone involved and is considered best practice. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l > F: 804-765-5582 l dkboyd@chs.net > > > > > > > > "Marcia Funk" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/06/2010 05:23 PM > > To > "Mark Tarango" , "Soo-Jin Cho" > > cc > "histonet@lists.utsouthwestern.edu" > Subject > Re: [Histonet] Policy on Floaters > > > > > > > Floaters > Yes, you are so right, for patient safety and your safety, policy is a > must. Protects you and the patient. > Marcia > > > Marcia Funk > Histology Laboratory > Mercy Medical Center North Iowa > Mason City, IA, 50401 > 641-422-7907 > > >>>> Mark Tarango 01/06/2010 2:21 PM >>> > Your goal is not to have floaters. If you get one, your policy should set > out to determine the cause of these incidents. You should track who did > it > (in a spreadsheet), where it happened (grossing, embedding, cutting...). > Then you should have a meeting every so often with people from the lab and > some pathologists where you go over all the incidents, brainstorm for > corrective actions, and decide about what you can do differently. > > Make sure you have the techs invovled or it won't be very effective. > > Mark > > > > > On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin > wrote: > >> Hello, I'm a resident at UCSF currently working on a QA/QI project >> regarding floaters, with the ultimate goal of formulating a departmental >> policy regarding floaters. Despite extensive searching on the internet > and >> the Histonet archives, I have not found any concrete examples of > policies at >> other institutions and was hoping someone could help me out in this > regard. >> Thank you in advance for your help. >> >> Most sincerely, >> Soo-Jin Cho >> Anatomic Pathology >> University of California, San Francisco >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Timothy.Morken <@t> ucsfmedctr.org Thu Jan 7 11:19:27 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Jan 7 11:19:34 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: References: <4B44B8D4.9B87.00AC.0@mercyhealth.com> <7722595275A4DD4FA225B92CDBF174A1E8C9F7E0F3@EXC-MBX3.cfs.le.ac.uk> Message-ID: <1AAF670737F193429070841C6B2ADD4C01216DD4F7@EXMBMCB15.ucsfmedicalcenter.org> I assure you "floaters" are not trivial in a diagnostic setting. Imagine a case biopsied for suspected melanoma. The tissue appears clear of melanoma but on the edge there is a small piece that is suspicious. But other slides do not show that piece and it is not in the block. Was it cut through? Is it a floater? Is it real? Investigations ensue. Even genetic testing of the fragment may be done to resolve the issue. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Thursday, January 07, 2010 9:02 AM To: Edwards, Richard E.; 'DKBoyd@chs.net'; Marcia Funk Cc: histonet@lists.utsouthwestern.edu; Cho, Soo-Jin Subject: RE: [Histonet] Policy on Floaters I second that question! Seems like a lot of hassle (documenting, holding meetings) over a bit of waxy tissue left in a water bath (mind you, this is coming from a research mind-set where we don't mind these things so much). :-) --On Thursday, January 07, 2010 1:34 PM +0000 "Edwards, Richard E." wrote: > So, what exactly is a "floater", an extra bit of a previous section > picked up with the next section in a dirty waterbath, or an obviously > extra identifiable piece of tissue included in/picked up in error during > grossing; bit of ambiguity in previous emails?. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > DKBoyd@chs.net Sent: 07 January 2010 13:18 > To: Marcia Funk > Cc: histonet@lists.utsouthwestern.edu; Soo-Jin Cho; > histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Policy > on Floaters > > Lots of traffic on this one. Yes you definitely need a policy. The > policy should define how you will "try" to eliminate floaters ie; clean > water bath after each block, spray/wipe/change paper towels after each > specimen while grossing etc. It also must include how it will be > resolved. This covers everyone involved and is considered best practice. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l > F: 804-765-5582 l dkboyd@chs.net > > > > > > > > "Marcia Funk" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/06/2010 05:23 PM > > To > "Mark Tarango" , "Soo-Jin Cho" > > cc > "histonet@lists.utsouthwestern.edu" > Subject > Re: [Histonet] Policy on Floaters > > > > > > > Floaters > Yes, you are so right, for patient safety and your safety, policy is a > must. Protects you and the patient. > Marcia > > > Marcia Funk > Histology Laboratory > Mercy Medical Center North Iowa > Mason City, IA, 50401 > 641-422-7907 > > >>>> Mark Tarango 01/06/2010 2:21 PM >>> > Your goal is not to have floaters. If you get one, your policy should set > out to determine the cause of these incidents. You should track who did > it > (in a spreadsheet), where it happened (grossing, embedding, cutting...). > Then you should have a meeting every so often with people from the lab and > some pathologists where you go over all the incidents, brainstorm for > corrective actions, and decide about what you can do differently. > > Make sure you have the techs invovled or it won't be very effective. > > Mark > > > > > On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin > wrote: > >> Hello, I'm a resident at UCSF currently working on a QA/QI project >> regarding floaters, with the ultimate goal of formulating a departmental >> policy regarding floaters. Despite extensive searching on the internet > and >> the Histonet archives, I have not found any concrete examples of > policies at >> other institutions and was hoping someone could help me out in this > regard. >> Thank you in advance for your help. >> >> Most sincerely, >> Soo-Jin Cho >> Anatomic Pathology >> University of California, San Francisco >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From VHammel <@t> primecare.org Thu Jan 7 11:43:52 2010 From: VHammel <@t> primecare.org (Hammel, Vicky) Date: Thu Jan 7 11:44:23 2010 Subject: [Histonet] CMV positive tissue Message-ID: <4F0B7161A6CD524FAD8017D52E155340090403EC@exchangent> What are labs using for positive tissue for CMV IHC? If anyone is interested in trading tissue please contact me. Thank you, Vicky Hammel From VHammel <@t> primecare.org Thu Jan 7 11:50:01 2010 From: VHammel <@t> primecare.org (Hammel, Vicky) Date: Thu Jan 7 11:50:34 2010 Subject: [Histonet] cmv positive tissue Message-ID: <4F0B7161A6CD524FAD8017D52E155340090403ED@exchangent> What are labs using for positive tissue for CMV IHC? If anyone is interested in trading tissue (slides or blocks) please contact me. Thank you, Vicky Hammel HTL, ASCP Pathology Technical Consultant vhammel@primecare.org St. Alexius Medical Center Histology Laboratory 900 east Broadway Bismarck, ND 58506 From marktarango <@t> gmail.com Thu Jan 7 12:12:54 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Jan 7 12:12:59 2010 Subject: [Histonet] cmv positive tissue In-Reply-To: <4F0B7161A6CD524FAD8017D52E155340090403ED@exchangent> References: <4F0B7161A6CD524FAD8017D52E155340090403ED@exchangent> Message-ID: <5b6eb13e1001071012x648dcb7ao84e1d26fdd1cbc08@mail.gmail.com> I can trade you. I have a few prepared control blocks that we use for IHC. I'd be willing to trade for malignant melanoma. In Seattle, we don't get enough sun to see many melanoma cases. If you know that its postive for hmb-45, mart-1/melan-a, and S100 it'd be my dream come true. Let me know! Thanks Mark Tarango HT(ASCP)QIHC Cellnetix Pathology 1124 Columbia Street, Suite 200 Seattle, Wa 98104 On Thu, Jan 7, 2010 at 9:50 AM, Hammel, Vicky wrote: > What are labs using for positive tissue for CMV IHC? If anyone is > interested > in trading tissue (slides or blocks) please contact me. > > Thank you, > > > > Vicky Hammel HTL, ASCP > > Pathology Technical Consultant > > > > vhammel@primecare.org > > > > St. Alexius Medical Center > > Histology Laboratory > > 900 east Broadway > > Bismarck, ND 58506 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From marktarango <@t> gmail.com Thu Jan 7 12:14:30 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Jan 7 12:14:34 2010 Subject: [Histonet] cmv positive tissue In-Reply-To: <5b6eb13e1001071012x648dcb7ao84e1d26fdd1cbc08@mail.gmail.com> References: <4F0B7161A6CD524FAD8017D52E155340090403ED@exchangent> <5b6eb13e1001071012x648dcb7ao84e1d26fdd1cbc08@mail.gmail.com> Message-ID: <5b6eb13e1001071014q68062266hb9bd97323666699b@mail.gmail.com> Sorry everyone, I didn't mean to send that to the whole histonet. Please respond privately Vicky. On Thu, Jan 7, 2010 at 10:12 AM, Mark Tarango wrote: > I can trade you. I have a few prepared control blocks that we use for > IHC. I'd be willing to trade for malignant melanoma. In Seattle, we don't > get enough sun to see many melanoma cases. If you know that its postive for > hmb-45, mart-1/melan-a, and S100 it'd be my dream come true. > > Let me know! > > Thanks > > Mark Tarango HT(ASCP)QIHC > Cellnetix Pathology > 1124 Columbia Street, Suite 200 > Seattle, Wa 98104 > On Thu, Jan 7, 2010 at 9:50 AM, Hammel, Vicky wrote: > >> What are labs using for positive tissue for CMV IHC? If anyone is >> interested >> in trading tissue (slides or blocks) please contact me. >> >> Thank you, >> >> >> >> Vicky Hammel HTL, ASCP >> >> Pathology Technical Consultant >> >> >> >> vhammel@primecare.org >> >> >> >> St. Alexius Medical Center >> >> Histology Laboratory >> >> 900 east Broadway >> >> Bismarck, ND 58506 >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From bill501 <@t> mindspring.com Thu Jan 7 12:41:50 2010 From: bill501 <@t> mindspring.com (Bill B.) Date: Thu Jan 7 12:42:01 2010 Subject: [Histonet] Policy on Floaters In-Reply-To: <1AAF670737F193429070841C6B2ADD4C01216DD4F7@EXMBMCB15.ucsfmedicalcenter.or g> References: <4B44B8D4.9B87.00AC.0@mercyhealth.com> <7722595275A4DD4FA225B92CDBF174A1E8C9F7E0F3@EXC-MBX3.cfs.le.ac.uk> <1AAF670737F193429070841C6B2ADD4C01216DD4F7@EXMBMCB15.ucsfmedicalcenter.or g> Message-ID: At 9:19 AM -0800 1/7/10, Morken, Tim wrote: >I assure you "floaters" are not trivial in a diagnostic setting. Imagine a case biopsied for suspected melanoma. The tissue appears clear of melanoma but on the edge there is a small piece that is suspicious. But other slides do not show that piece and it is not in the block. Was it cut through? Is it a floater? Is it real? Investigations ensue. Even genetic testing of the fragment may be done to resolve the issue. <<< Yes, but most floaters are obvious. Most common, IME, are chorionic villi. Granted a rare one can be a diagnostic conundrum and must be worked up appropriately. I think it a waste of time and resources to investigate and document every one encountered, beyond marking them on the slide as a floater. If they are becoming common then their source must be investigated. Bill From rsrichmond <@t> gmail.com Thu Jan 7 12:42:58 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Jan 7 12:43:01 2010 Subject: [Histonet] Re: Policy on Floaters Message-ID: Dr. Soo-Jin Cho asks: >>Hello, I'm a resident at UCSF currently working on a QA/QI project regarding floaters, with the ultimate goal of formulating a departmental policy regarding floaters. Despite extensive searching on the internet and the Histonet archives, I have not found any concrete examples of policies at other institutions and was hoping someone could help me out in this regard.<< Several people have already offered some very useful advice. From the pathologist's viewpoint, I'd distinguish between section floaters (which usually aren't the direct concern of the pathologist or pathologist's assistant) and tissue floaters (which definitely are). The usual cause of tissue floaters is carry-over of tissue fragments during grossing. I almost never have this problem myself - where I've seen problems is with an overloaded P.A. who's working too fast. I've always been ridiculed for proposing that it would be worthwhile to have about five sets of the basic dissecting tools (scalpel, scissors, ruler, tweezers, etc.) and toss them into a pot of water between each case, then stop and wash them off after five cases. (This arrangement would of course violate the Good Management Principle that a pathologist may have only one set of tools.) Certain cases - such as papillary urinary tract and ovarian tumors - are notorious creators of floaters and should be grossed last, or right before the placentas at any rate. The worst floater incident I've ever seen occurred in a small lab that mostly saw skin biopsies. A lens paper wrapper (this was over 20 years ago) came open in processing and spilled out the cell block contents from a peritoneal fluid specimen. The specimen contained papillary fragments from an ovarian tumor. I found those fragments in the slides from 26 different cases (fortunately none of them was diagnostically difficult). Follow-up with the attending physician found that he was totally surprised by the results - the cancer was quite unsuspected in a woman with rapidly progressing ascites. I was taught to write "floater" on the slide with an appropriate pen, and initial the slide also. The problem should almost never be mentioned in the pathologist's report. Bob Richmond Samurai Pathologist Knoxville TN From gvdobbin <@t> ihis.org Thu Jan 7 12:49:07 2010 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Jan 7 12:49:27 2010 Subject: [Histonet] pH of 10% NBF Message-ID: Hi Folks, If the pH of our 10% Neutral Buffered Formalin is reading 7.01 (recycled) and 7.12 (made from new formaldehyde), should I try to get to 7.2? If yes-how- add sodium bicarbonate? I look forward to your responses. Warm regards, Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From Erik.Dokken <@t> onassignment.com Thu Jan 7 12:50:40 2010 From: Erik.Dokken <@t> onassignment.com (Erik Dokken) Date: Thu Jan 7 12:53:44 2010 Subject: [Histonet] Histotech Needed In Seattle In-Reply-To: <201001071803.o07I1U6d007427@smtp12.onasgn.net> Message-ID: On Assignment Healthcare in Seattle is currently looking for a qualified Histotech to complete a one month contract with one of our clients in Seattle, WA. If you or someone that you know is interested in receiving additional information about this position please do not hesitate to contact us. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, January 07, 2010 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 74, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC recommendations paper - FYI (Liz Chlipala) 2. Policy on Floaters (Cho, Soo-Jin) 3. NYS Licensing Examination Update (NYSHisto) 4. Re: Policy on Floaters (Rene J Buesa) 5. Re: Policy on Floaters (Bill) 6. RE: Policy on Floaters (Blazek, Linda) 7. Re: Policy on Floaters (Mark Tarango) 8. special stains (Jennifer Campbell) 9. RE: Policy on Floaters (Terri Braud) 10. Re: Policy on Floaters (Rene J Buesa) 11. Re: Policy on Floaters (Marcia Funk) 12. Cytokeratin 17 (Nicholas David Evans) 13. Re: Policy on Floaters (DKBoyd@chs.net) 14. RE: Policy on Floaters (Edwards, Richard E.) 15. osteopontin antibody (Michele Wich) 16. getting rid of surplus equipment-Source? (Madary, Joseph) 17. RE: Policy on Floaters (Merced M Leiker) 18. RE: Policy on Floaters (Morken, Tim) 19. CMV positive tissue (Hammel, Vicky) 20. cmv positive tissue (Hammel, Vicky) ---------------------------------------------------------------------- Message: 1 Date: Wed, 6 Jan 2010 11:30:22 -0700 From: "Liz Chlipala" Subject: [Histonet] IHC recommendations paper - FYI To: Message-ID: Content-Type: text/plain; charset="us-ascii" A lot of people have asked me for the paper that I mentioned in my e-mail yesterday. I think I found it on the CAP website, but here is the papers title and authors. It's a review article. Its helped me a lot with IHC validation. VM114 Standardization and Troubleshooting Immunohistochemistry for Pathologists Neal S. Goldstein, MD October 2, 2007 8:30 AM - Noon Recommendations for Improved Standardization of Immunohistochemistry Neal S. Goldstein, MD, Stephen M. Hewitt, MD, PhD, Clive R. Taylor, MD, DPhil, Hadi Yaziji, MD, David G. Hicks, MD, and Members of Ad-Hoc Committee On Immunohistochemistry Standardization Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 ------------------------------ Message: 2 Date: Wed, 6 Jan 2010 10:45:53 -0800 From: "Cho, Soo-Jin" Subject: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" Message-ID: <622D25C2D399AC40A7CDCF12351B5151FB8CA811@exmbmcb12.ucsfmedicalcenter.org> Content-Type: text/plain; charset=iso-8859-1 Hello, I'm a resident at UCSF currently working on a QA/QI project regarding floaters, with the ultimate goal of formulating a departmental policy regarding floaters. Despite extensive searching on the internet and the Histonet archives, I have not found any concrete examples of policies at other institutions and was hoping someone could help me out in this regard. Thank you in advance for your help. Most sincerely, Soo-Jin Cho Anatomic Pathology University of California, San Francisco ------------------------------ Message: 3 Date: Wed, 6 Jan 2010 11:33:57 -0800 (PST) From: NYSHisto Subject: [Histonet] NYS Licensing Examination Update To: Histonet List Cc: Amy Farnan Message-ID: <969352.91010.qm@web58803.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Everyone We have excellent news......!! we?have just received this update from the New York State Office of Professions regarding the examination procedures and eligibility for licensing in NYS, Please see below.? Since this is "hot off the press" information, I am sure there will be questions but please be patient as the procedures are formally written and posted on the website (http://www.op.nysed.gov/).??For those of you who want to be updated regularly on whats going on in NY, please join the New York State Histotechnological Society message board at:?http://tech.groups.yahoo.com/group/NYSHS1972/?If you have questions regarding licensing in NYS please feel free to contact us by email. Regards Luis Chiriboga & Amy Farnan,? NYSHS Legislative Committee ? ------- Within the past few weeks, our contract with ASCP for our candidates to use the ASCP exam for licensure as a certified histological technician has been approved by the State Comptroller and we have begun to implement the use of this examination.? Applicants for licensure as certified histological technician will use the same examination, during the five year period for the contract, as is used by ASCP for their certification purposes.? But, these are separate procedures.? Applicants for licensure pay the fee for the exam that is established by our contract, have scores reported directly to us, and have the right to take the examination as often as needed.? The same persons may also want a certificate from ASCP.? If so, they will have to apply to ASCP for this certificate, pay their fee, meet their requirements, and take the examination according to their specifications.? These are totally separate processes.? We simply contract for the examination and do not endorse the ASCP certification.? For persons who have applied for this license recently, we may have issued a limited permit and they will have to take the examination prior to licensure during the one year period that the permit is valid.? There are also some persons who have received a limited license as a certified histological technician.? These persons will have to meet not only the examination, but the full education requirements.? For those persons who hold a limited permit and did take and pass the examination after September 1, 2001, their examination results may be used for licensure purposes and they will not have to retake the examination.? Anyone who took the examination prior to September 1, 2001 will have to take the examination now for it to be used for licensure.? We have received a number of score reports from ASCP on applicants who now hold a limited permit, and we will now be processing the applications for licensure, as long as all requirements are met. Finally, a note on another topic.? There are many persons who work as histological technicians who were originally licensed as certified clinical laboratory technicians and who may also be licensed as certified histological technicians.? The reregistration period for the license as a certified clinical laboratory technician may now be expiring.? These person have the choice of reregistering as a certified clinical laboratory technician or placing that registration in an "inactive status," (and they must inform us if that is their intention) or of maintaining both registrations, or of placing their registration, when it comes up, on an "inactive status."? Both licenses will continue unexpired for life unless removed by disciplinary action, but the person may only practice in the areas authorized under the scope of the profession in which they are licensed and registered.? The scope of both licenses authorize the practice of histological technician. Kathleen Dr. Kathleen M. Doyle Executive Secretary kdoyle2@mail.nysed.gov 518-474-3817, ext. 150 ------- Luis Chiriboga Ph.D. Assistant Professor of Pathology Vice President New York State Histotechnological Society NYSHS Website www.nyhisto.org NYSHS Message Board http://tech.groups.yahoo.com/group/NYSHS1972/ ------------------------------ Message: 4 Date: Wed, 6 Jan 2010 12:02:21 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" , Soo-JinCho Message-ID: <483213.60612.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 It is difficult for you to find a "policy on floaters" because any lab will try to not get any floater. It could be said that 1 floater is 1 too many. The goal is "0" floaters. Ren? J. --- On Wed, 1/6/10, Cho, Soo-Jin wrote: From: Cho, Soo-Jin Subject: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, January 6, 2010, 1:45 PM Hello, I'm a resident at UCSF currently working on a QA/QI project regarding floaters, with the ultimate goal of formulating a departmental policy regarding floaters.? Despite extensive searching on the internet and the Histonet archives, I have not found any concrete examples of policies at other institutions and was hoping someone could help me out in this regard.? Thank you in advance for your help. Most sincerely, Soo-Jin Cho Anatomic Pathology University of California, San Francisco _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 6 Jan 2010 14:12:58 -0600 From: Bill Subject: Re: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" At 12:02 PM -0800 1/6/10, Rene J Buesa wrote: >It is difficult for you to find a "policy on floaters" because any lab will try to not get any floater. >It could be said that 1 floater is 1 too many. The goal is "0" floaters. Since no one is perfect and floaters WILL happen at every lab, what do you do when one raises its ugly head? Bill B ------------------------------ Message: 6 Date: Wed, 6 Jan 2010 15:17:25 -0500 From: "Blazek, Linda" Subject: RE: [Histonet] Policy on Floaters To: 'Bill' , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E390E92C0DA14@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Document it and see it there is a trend. Linda B -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Wednesday, January 06, 2010 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Policy on Floaters At 12:02 PM -0800 1/6/10, Rene J Buesa wrote: >It is difficult for you to find a "policy on floaters" because any lab will try to not get any floater. >It could be said that 1 floater is 1 too many. The goal is "0" floaters. Since no one is perfect and floaters WILL happen at every lab, what do you do when one raises its ugly head? Bill B _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 6 Jan 2010 12:21:49 -0800 From: Mark Tarango Subject: Re: [Histonet] Policy on Floaters To: "Cho, Soo-Jin" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <5b6eb13e1001061221h32020c6fhf38e09d513f6d86f@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Your goal is not to have floaters. If you get one, your policy should set out to determine the cause of these incidents. You should track who did it (in a spreadsheet), where it happened (grossing, embedding, cutting...). Then you should have a meeting every so often with people from the lab and some pathologists where you go over all the incidents, brainstorm for corrective actions, and decide about what you can do differently. Make sure you have the techs invovled or it won't be very effective. Mark On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin wrote: > Hello, I'm a resident at UCSF currently working on a QA/QI project > regarding floaters, with the ultimate goal of formulating a departmental > policy regarding floaters. Despite extensive searching on the internet and > the Histonet archives, I have not found any concrete examples of policies at > other institutions and was hoping someone could help me out in this regard. > Thank you in advance for your help. > > Most sincerely, > Soo-Jin Cho > Anatomic Pathology > University of California, San Francisco > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Wed, 6 Jan 2010 13:01:19 -0800 From: "Jennifer Campbell" Subject: [Histonet] special stains To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF82FA87B@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="us-ascii" I couldn't agree with you more, Liz! I am from a GLP lab as well and we have to undergo validation for every single new piece of equipment we acquire otherwise we are not considered GLP-compliant. Who is to say that your new stainer is going to stain slides in the same manner as your old stainer?--which is exactly why you have to validate your equipment and ensure that it is performing what the user has programmed it to perform and giving you accurate and consistent results. And we re-validate any time a change is made as well, whether we switch equipment or make a change to a staining protocol. We have special forms to document staining results and access to runtime logs on our stainer which allows us to ensure that the stainer carried out the correct protocol steps. Jen ------------------------------ Message: 9 Date: Wed, 6 Jan 2010 16:08:09 -0500 From: "Terri Braud" Subject: RE: [Histonet] Policy on Floaters To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Ok. I have to wade in. We actually have a policy on floaters. Our policy calls for the pathologist to circle the floater on the slide and mark it as a floater with a cell marking pen. That way the "floater" is identified forever on the permanent slide. He/she will notify the Supv or lead tech to investigate. If the floater is located within the block, then we dig it out of the block. We record the slide I.D., floater, and resolution on the daily stain qc sheet. Unless we have an ongoing problem, we don't track these any further but the data is there to trend, and if necessary, include in any QC, CQI, QA program. Hope this helps, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax From: Cho, Soo-Jin Subject: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, January 6, 2010, 1:45 PM Hello, I'm a resident at UCSF currently working on a QA/QI project regarding floaters, with the ultimate goal of formulating a departmental policy regarding floaters.? Despite extensive searching on the internet and the Histonet archives, I have not found any concrete examples of policies at other institutions and was hoping someone could help me out in this regard.? Thank you in advance for your help. Most sincerely, Soo-Jin Cho Anatomic Pathology University of California, San Francisco _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ------------------------------ Message: 10 Date: Wed, 6 Jan 2010 13:54:36 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" , Bill Message-ID: <449264.32138.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You document it in the QA file and prepare a follow up in the PI program. Ren? J. --- On Wed, 1/6/10, Bill wrote: From: Bill Subject: Re: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, January 6, 2010, 3:12 PM At 12:02 PM -0800 1/6/10, Rene J Buesa wrote: >It is difficult for you to find a "policy on floaters" because any lab will try to not get any floater. >It could be said that 1 floater is 1 too many. The goal is "0" floaters. Since no one is perfect and floaters WILL happen at every lab, what do you do when one raises its ugly head? Bill B _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 06 Jan 2010 17:22:44 -0500 From: "Marcia Funk" Subject: Re: [Histonet] Policy on Floaters To: "Mark Tarango" , "Soo-Jin Cho" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <4B44B8D4.9B87.00AC.0@mercyhealth.com> Content-Type: text/plain; charset=US-ASCII Floaters Yes, you are so right, for patient safety and your safety, policy is a must. Protects you and the patient. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> Mark Tarango 01/06/2010 2:21 PM >>> Your goal is not to have floaters. If you get one, your policy should set out to determine the cause of these incidents. You should track who did it (in a spreadsheet), where it happened (grossing, embedding, cutting...). Then you should have a meeting every so often with people from the lab and some pathologists where you go over all the incidents, brainstorm for corrective actions, and decide about what you can do differently. Make sure you have the techs invovled or it won't be very effective. Mark On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin wrote: > Hello, I'm a resident at UCSF currently working on a QA/QI project > regarding floaters, with the ultimate goal of formulating a departmental > policy regarding floaters. Despite extensive searching on the internet and > the Histonet archives, I have not found any concrete examples of policies at > other institutions and was hoping someone could help me out in this regard. > Thank you in advance for your help. > > Most sincerely, > Soo-Jin Cho > Anatomic Pathology > University of California, San Francisco > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 6 Jan 2010 15:32:40 -0800 (PST) From: "Nicholas David Evans" Subject: [histonet] Cytokeratin 17 To: Message-ID: <74C8CA0453A74B188D9C5FB3E7CDC247@DellDesktop2> Content-Type: text/plain; charset="us-ascii" Dear all, Can anyone suggest a decent, reliable antibody to mouse cytokeratin 17 (polyclonal or monoclonal is fine) I could buy? There are many on the market, but I want to be sure it works. Best wishes Nick ------------------------------ Message: 13 Date: Thu, 7 Jan 2010 08:18:07 -0500 From: DKBoyd@chs.net Subject: Re: [Histonet] Policy on Floaters To: "Marcia Funk" Cc: "histonet@lists.utsouthwestern.edu" , Soo-Jin Cho , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Lots of traffic on this one. Yes you definitely need a policy. The policy should define how you will "try" to eliminate floaters ie; clean water bath after each block, spray/wipe/change paper towels after each specimen while grossing etc. It also must include how it will be resolved. This covers everyone involved and is considered best practice. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Marcia Funk" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/06/2010 05:23 PM To "Mark Tarango" , "Soo-Jin Cho" cc "histonet@lists.utsouthwestern.edu" Subject Re: [Histonet] Policy on Floaters Floaters Yes, you are so right, for patient safety and your safety, policy is a must. Protects you and the patient. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> Mark Tarango 01/06/2010 2:21 PM >>> Your goal is not to have floaters. If you get one, your policy should set out to determine the cause of these incidents. You should track who did it (in a spreadsheet), where it happened (grossing, embedding, cutting...). Then you should have a meeting every so often with people from the lab and some pathologists where you go over all the incidents, brainstorm for corrective actions, and decide about what you can do differently. Make sure you have the techs invovled or it won't be very effective. Mark On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin wrote: > Hello, I'm a resident at UCSF currently working on a QA/QI project > regarding floaters, with the ultimate goal of formulating a departmental > policy regarding floaters. Despite extensive searching on the internet and > the Histonet archives, I have not found any concrete examples of policies at > other institutions and was hoping someone could help me out in this regard. > Thank you in advance for your help. > > Most sincerely, > Soo-Jin Cho > Anatomic Pathology > University of California, San Francisco > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 14 Date: Thu, 7 Jan 2010 13:34:08 +0000 From: "Edwards, Richard E." Subject: RE: [Histonet] Policy on Floaters To: "'DKBoyd@chs.net'" , Marcia Funk Cc: "histonet@lists.utsouthwestern.edu" , Soo-Jin Cho , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E0F3@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" So, what exactly is a "floater", an extra bit of a previous section picked up with the next section in a dirty waterbath, or an obviously extra identifiable piece of tissue included in/picked up in error during grossing; bit of ambiguity in previous emails?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: 07 January 2010 13:18 To: Marcia Funk Cc: histonet@lists.utsouthwestern.edu; Soo-Jin Cho; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Policy on Floaters Lots of traffic on this one. Yes you definitely need a policy. The policy should define how you will "try" to eliminate floaters ie; clean water bath after each block, spray/wipe/change paper towels after each specimen while grossing etc. It also must include how it will be resolved. This covers everyone involved and is considered best practice. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Marcia Funk" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/06/2010 05:23 PM To "Mark Tarango" , "Soo-Jin Cho" cc "histonet@lists.utsouthwestern.edu" Subject Re: [Histonet] Policy on Floaters Floaters Yes, you are so right, for patient safety and your safety, policy is a must. Protects you and the patient. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> Mark Tarango 01/06/2010 2:21 PM >>> Your goal is not to have floaters. If you get one, your policy should set out to determine the cause of these incidents. You should track who did it (in a spreadsheet), where it happened (grossing, embedding, cutting...). Then you should have a meeting every so often with people from the lab and some pathologists where you go over all the incidents, brainstorm for corrective actions, and decide about what you can do differently. Make sure you have the techs invovled or it won't be very effective. Mark On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin wrote: > Hello, I'm a resident at UCSF currently working on a QA/QI project > regarding floaters, with the ultimate goal of formulating a departmental > policy regarding floaters. Despite extensive searching on the internet and > the Histonet archives, I have not found any concrete examples of policies at > other institutions and was hoping someone could help me out in this regard. > Thank you in advance for your help. > > Most sincerely, > Soo-Jin Cho > Anatomic Pathology > University of California, San Francisco > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 7 Jan 2010 09:45:00 -0600 From: "Michele Wich" Subject: [Histonet] osteopontin antibody To: Message-ID: <62A8156F8071C8439080D626DF8C33A6CEB2F0@wave-mail.7thwave.local> Content-Type: text/plain; charset="us-ascii" Can anyone recommend an osteopontin antibody that works in FFPE mouse tissue? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ------------------------------ Message: 16 Date: Thu, 7 Jan 2010 11:38:27 -0500 From: "Madary, Joseph" Subject: [Histonet] getting rid of surplus equipment-Source? To: Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A130102DEC4@MD1EV002.medimmune.com> Content-Type: text/plain; charset="US-ASCII" Does anyone have a good source to get rid of equipment that is still good and maybe our lab can make a few bucks in the process? The last time I did this I was told the processor and stainer I gave away would be going to some charity and of course it did not I found out later. These items are off the books, maybe in need of a small repair. We have no room and the equipment is just been depreciated such that if we can get a credit or something for our lab as a trade in. Specifically we have an RMS microtome in perfect condition used for 6 months and put away. The other is a Leica Embedder that does everyting but dispense paraffin. We were using a separate paraffin dispenser and it was fine, but finally got another embedder. Anyway, vendors welcome. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ------------------------------ Message: 17 Date: Thu, 07 Jan 2010 12:01:42 -0500 From: Merced M Leiker Subject: RE: [Histonet] Policy on Floaters To: "Edwards, Richard E." , "'DKBoyd@chs.net'" , Marcia Funk Cc: histonet@lists.utsouthwestern.edu, Soo-Jin Cho Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed I second that question! Seems like a lot of hassle (documenting, holding meetings) over a bit of waxy tissue left in a water bath (mind you, this is coming from a research mind-set where we don't mind these things so much). :-) --On Thursday, January 07, 2010 1:34 PM +0000 "Edwards, Richard E." wrote: > So, what exactly is a "floater", an extra bit of a previous section > picked up with the next section in a dirty waterbath, or an obviously > extra identifiable piece of tissue included in/picked up in error during > grossing; bit of ambiguity in previous emails?. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > DKBoyd@chs.net Sent: 07 January 2010 13:18 > To: Marcia Funk > Cc: histonet@lists.utsouthwestern.edu; Soo-Jin Cho; > histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Policy > on Floaters > > Lots of traffic on this one. Yes you definitely need a policy. The > policy should define how you will "try" to eliminate floaters ie; clean > water bath after each block, spray/wipe/change paper towels after each > specimen while grossing etc. It also must include how it will be > resolved. This covers everyone involved and is considered best practice. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l > F: 804-765-5582 l dkboyd@chs.net > > > > > > > > "Marcia Funk" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/06/2010 05:23 PM > > To > "Mark Tarango" , "Soo-Jin Cho" > > cc > "histonet@lists.utsouthwestern.edu" > Subject > Re: [Histonet] Policy on Floaters > > > > > > > Floaters > Yes, you are so right, for patient safety and your safety, policy is a > must. Protects you and the patient. > Marcia > > > Marcia Funk > Histology Laboratory > Mercy Medical Center North Iowa > Mason City, IA, 50401 > 641-422-7907 > > >>>> Mark Tarango 01/06/2010 2:21 PM >>> > Your goal is not to have floaters. If you get one, your policy should set > out to determine the cause of these incidents. You should track who did > it > (in a spreadsheet), where it happened (grossing, embedding, cutting...). > Then you should have a meeting every so often with people from the lab and > some pathologists where you go over all the incidents, brainstorm for > corrective actions, and decide about what you can do differently. > > Make sure you have the techs invovled or it won't be very effective. > > Mark > > > > > On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin > wrote: > >> Hello, I'm a resident at UCSF currently working on a QA/QI project >> regarding floaters, with the ultimate goal of formulating a departmental >> policy regarding floaters. Despite extensive searching on the internet > and >> the Histonet archives, I have not found any concrete examples of > policies at >> other institutions and was hoping someone could help me out in this > regard. >> Thank you in advance for your help. >> >> Most sincerely, >> Soo-Jin Cho >> Anatomic Pathology >> University of California, San Francisco >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 18 Date: Thu, 7 Jan 2010 09:19:27 -0800 From: "Morken, Tim" Subject: RE: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4C01216DD4F7@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii I assure you "floaters" are not trivial in a diagnostic setting. Imagine a case biopsied for suspected melanoma. The tissue appears clear of melanoma but on the edge there is a small piece that is suspicious. But other slides do not show that piece and it is not in the block. Was it cut through? Is it a floater? Is it real? Investigations ensue. Even genetic testing of the fragment may be done to resolve the issue. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Thursday, January 07, 2010 9:02 AM To: Edwards, Richard E.; 'DKBoyd@chs.net'; Marcia Funk Cc: histonet@lists.utsouthwestern.edu; Cho, Soo-Jin Subject: RE: [Histonet] Policy on Floaters I second that question! Seems like a lot of hassle (documenting, holding meetings) over a bit of waxy tissue left in a water bath (mind you, this is coming from a research mind-set where we don't mind these things so much). :-) --On Thursday, January 07, 2010 1:34 PM +0000 "Edwards, Richard E." wrote: > So, what exactly is a "floater", an extra bit of a previous section > picked up with the next section in a dirty waterbath, or an obviously > extra identifiable piece of tissue included in/picked up in error during > grossing; bit of ambiguity in previous emails?. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > DKBoyd@chs.net Sent: 07 January 2010 13:18 > To: Marcia Funk > Cc: histonet@lists.utsouthwestern.edu; Soo-Jin Cho; > histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Policy > on Floaters > > Lots of traffic on this one. Yes you definitely need a policy. The > policy should define how you will "try" to eliminate floaters ie; clean > water bath after each block, spray/wipe/change paper towels after each > specimen while grossing etc. It also must include how it will be > resolved. This covers everyone involved and is considered best practice. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l > F: 804-765-5582 l dkboyd@chs.net > > > > > > > > "Marcia Funk" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/06/2010 05:23 PM > > To > "Mark Tarango" , "Soo-Jin Cho" > > cc > "histonet@lists.utsouthwestern.edu" > Subject > Re: [Histonet] Policy on Floaters > > > > > > > Floaters > Yes, you are so right, for patient safety and your safety, policy is a > must. Protects you and the patient. > Marcia > > > Marcia Funk > Histology Laboratory > Mercy Medical Center North Iowa > Mason City, IA, 50401 > 641-422-7907 > > >>>> Mark Tarango 01/06/2010 2:21 PM >>> > Your goal is not to have floaters. If you get one, your policy should set > out to determine the cause of these incidents. You should track who did > it > (in a spreadsheet), where it happened (grossing, embedding, cutting...). > Then you should have a meeting every so often with people from the lab and > some pathologists where you go over all the incidents, brainstorm for > corrective actions, and decide about what you can do differently. > > Make sure you have the techs invovled or it won't be very effective. > > Mark > > > > > On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin > wrote: > >> Hello, I'm a resident at UCSF currently working on a QA/QI project >> regarding floaters, with the ultimate goal of formulating a departmental >> policy regarding floaters. Despite extensive searching on the internet > and >> the Histonet archives, I have not found any concrete examples of > policies at >> other institutions and was hoping someone could help me out in this > regard. >> Thank you in advance for your help. >> >> Most sincerely, >> Soo-Jin Cho >> Anatomic Pathology >> University of California, San Francisco >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Thu, 7 Jan 2010 11:43:52 -0600 From: "Hammel, Vicky" Subject: [Histonet] CMV positive tissue To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <4F0B7161A6CD524FAD8017D52E155340090403EC@exchangent> Content-Type: text/plain What are labs using for positive tissue for CMV IHC? If anyone is interested in trading tissue please contact me. Thank you, Vicky Hammel ------------------------------ Message: 20 Date: Thu, 7 Jan 2010 11:50:01 -0600 From: "Hammel, Vicky" Subject: [Histonet] cmv positive tissue To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <4F0B7161A6CD524FAD8017D52E155340090403ED@exchangent> Content-Type: text/plain What are labs using for positive tissue for CMV IHC? If anyone is interested in trading tissue (slides or blocks) please contact me. Thank you, Vicky Hammel HTL, ASCP Pathology Technical Consultant vhammel@primecare.org St. Alexius Medical Center Histology Laboratory 900 east Broadway Bismarck, ND 58506 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 74, Issue 6 *************************************** From NMargaryan <@t> childrensmemorial.org Thu Jan 7 13:26:28 2010 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Jan 7 13:31:33 2010 Subject: [Histonet] phosphoSMAD-2 In-Reply-To: <389a17a7-1ff3-40da-87ca-3f905c9dae89@CMHHTCA01.childrensmemorial.org> References: <389a17a7-1ff3-40da-87ca-3f905c9dae89@CMHHTCA01.childrensmemorial.org> Message-ID: Hi histonetters, I am looking for a good Ab for IHC for phospho-SMAD-2 on mouse tissue. Any suggestions on company and protocol is appreciated. Thanks in advance, Naira From sbreeden <@t> nmda.nmsu.edu Thu Jan 7 14:13:18 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Jan 7 14:13:23 2010 Subject: [Histonet] California Histotech? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46D51@nmdamailsvr.nmda.ad.nmsu.edu> I need some assistance and am looking for a contact HT in the San Diego area. If that's you and you're willing to answer a couple questions of a general histology nature, please reply to me directly (not via Histonet). This is not a vendor issue and is not a job-search. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From AnthonyH <@t> chw.edu.au Thu Jan 7 16:08:09 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jan 7 16:08:20 2010 Subject: [Histonet] pH of 10% NBF In-Reply-To: Message-ID: Greg, A pH "around" 7 is the aim: Abler et al (J Histotechnol 6(4):181-184,1983) surveyed several neutral buffered formalin solutions received from different laboratories and found a great variation in osmolarity, pH, buffering capacity and formaldehyde concentration. They recommend the following quality control parameters be routinely checked on neutral buffered formalin solutions prepared and used in histopathology laboratories: Osmolarity 1,000 - 1,600 milliosmols pH 6.5 - 7.5 Buffering Capacity * Greater than 0.75ml Formaldehyde Concentration 3 - 5%w/v * Buffering Capacity is obtained by titrating the amount of 0.1N hydrochloric acid required to lower the pH of the solution to 2 Comparing the osmolarity in the above table with that recommended for Millonig's buffered formalin obviously indicates some disagreement. The importance of osmolarity in fixation is debatable. Carson et al (Am J.Clin Pathol 59:365, 1973) recommend that Millonig's Phosphate Buffered Formalin should have a pH of 7.4 and the effective osmolarity is about 290mOsM. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Friday, 8 January 2010 5:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] pH of 10% NBF Hi Folks, If the pH of our 10% Neutral Buffered Formalin is reading 7.01 (recycled) and 7.12 (made from new formaldehyde), should I try to get to 7.2? If yes-how- add sodium bicarbonate? I look forward to your responses. Warm regards, Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From mjamison <@t> caissonlabs.com Thu Jan 7 17:29:04 2010 From: mjamison <@t> caissonlabs.com (Michelle Jamison) Date: Thu Jan 7 17:29:43 2010 Subject: [Histonet] GUS staining on parifin thin sections, or infiltration os stain into developing embryos Message-ID: <5C39B8D5B9094B978804F9E7238AF9C3@GEMINILAB> Hi All I have been trying to stain for GUS expression (X-Gluc) in developing seeds of Arabidopsis. I have good expression on my positive control (a "housekeeping gene) except for the developing embryos! And forget about those samples with the specific gene in the embryos. One of the things I will be trying is thin sectioning then staining. Possibly the enzyme will be compromised? Does anyone have experience with this sort of thing? Please let me know your thoughts and or experiences. Thank you Michelle Michelle Jamison Cytology Caisson labs 1740 Research Parkway North Logan Utah 84341 435.755.7617 ext 109 From jkiernan <@t> uwo.ca Fri Jan 8 01:10:43 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Jan 8 01:10:48 2010 Subject: [Histonet] GUS staining on parifin thin sections, or infiltration os stain into developing embryos Message-ID: The following three papers indicate that attention to technical details is important in the detection of beta-glucuronidase as a reporter gene. - - - 1. Caissard JC, Guivarch A, Rembur J, Azmi A, Chriqui D (1994) Spurious localizations of diX-indigo microcrystals generated by the histochemical GUS assay. Transgenic Research 3: 176-181. 2. Tague BW, Gallant P, Goodman HM (1997) Expression analysis of an Arabidopsis C2H2 zinc finger protein gene. Plant Molecular Biology 32: 785-796. 3. Guivarch A, Caissard JC, Azmi A, Elmayan T, Chriqui D, Tepfer M 1996 In situ detection of expression of the gus reporter gene transgenic plants: Ten years of blue genes Transgenic Research 5 (5): 281-288 SEP 1996 Abstract: Among the methods now available to localize the sites of gene expression in plant materials, reporter genes based on the gus (uidA) gene of Escherichia coli, which encodes a beta-glucuronidase (E.C. 3.2.1.31; GUS), have been the most widely used during the last ten years. The apparent simplicity of the histochemical GUS assay has been a major factor in the increase in articles using gus genes. However, over the last four years, there have been occasional reports expressing doubts concerning the specificity of the observed localizations, based on discrepancies between results obtained with GUS histochemistry and immunocytochemistry and/or in situ hybridization. This brief review compares the results obtained with immunocytochemistry with those obtained with various GUS substrates for histochemical studies. Certain sources of artefact are discussed, as are the limits that should be imposed on interpretation of GUS histochemistry results at the organ, tissue and cell levels. - - - X-gal, X-gluc etc are nicknames for bromo-chloro-indoxyl glycosides, which can generate insoluble indigo-like pigments after enzyme-catalysed hydrolysis followed immediately by oxidation. The same X-* substrates are used also in methods that trap the products as insoluble azo dyes or as formazans. These methods are from the Golden Age of enzyme activity histochemistry (1960s and 1970s). A recent review of indigogenic substrates (Indigogenic substrates for the detection and localization of enzymes. Biotechnic & Histochemistry 82, 73-103, 2007) covers many sources of false-positive and false-negative artifacts. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Michelle Jamison Date: Thursday, January 7, 2010 18:30 Subject: [Histonet] GUS staining on parifin thin sections, or infiltration os stain into developing embryos To: histonet@lists.utsouthwestern.edu > Hi All > I have been trying to stain for GUS expression (X-Gluc) in > developing seeds of Arabidopsis. I have good expression on > my positive control (a "housekeeping gene) except for the > developing embryos! And forget about those samples with > the specific gene in the embryos. One of the things I will > be trying is thin sectioning then staining. Possibly the > enzyme will be compromised? > Does anyone have experience with this sort of thing? > Please let me know your thoughts and or experiences. > Thank you > Michelle > > > > Michelle Jamison > Cytology > Caisson labs > 1740 Research Parkway > North Logan Utah > 84341 > 435.755.7617 ext 109 From mcauliff <@t> umdnj.edu Fri Jan 8 09:52:19 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jan 8 09:50:17 2010 Subject: [Histonet] pH of 10% NBF In-Reply-To: References: Message-ID: <4B4754B3.40004@umdnj.edu> Your pH meter, unless it is a very sensitive (expensive) meter with a very good electrode and has been properly calibrated that day is probably off at least 0.05 - 0.1 pH units so don't worry. Geoff Greg Dobbin wrote: > Hi Folks, > If the pH of our 10% Neutral Buffered Formalin is reading 7.01 > (recycled) and 7.12 (made from new formaldehyde), should I try to get to > 7.2? If yes-how- add sodium bicarbonate? > I look forward to your responses. > Warm regards, > Greg > > Greg Dobbin, R.T. > Chief Technologist, Anatomic Pathology > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > "I find that the harder I work, the > more luck I seem to have." > - Thomas Jefferson > > > ------------------------- > Statement of Confidentiality > This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From lyonm <@t> upstate.edu Fri Jan 8 10:54:29 2010 From: lyonm <@t> upstate.edu (Michael J. Lyon, Ph.D.) Date: Fri Jan 8 10:51:21 2010 Subject: [Histonet] Reichert-Jung 2050 service Message-ID: <000301ca9083$3e57afd0$bb070f70$@edu> We have a Reichert-Jung 2050 that needs service. Any suggestions welcome. Michael J. Lyon, Ph.D. Otolaryngology Research Lab SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice 315-464-7253 Fax 315-464-5572 From collette2 <@t> mail.llnl.gov Fri Jan 8 12:28:31 2010 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Fri Jan 8 12:28:50 2010 Subject: [Histonet] shipping slides, elementary question Message-ID: Happy Friday everyone, I have a very basic question about shipping slides (mouse tissue, non-biohaz). I am planning to ship a bunch to a collaborator, on the order of a few hundred. I have black hinged cardboard/wood 100-slide boxes, similar to https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=48452-001&inE=1&highlight=48452-001 will these be sufficient for shipping (provided I ensure they stay closed during transit) to avoid breakage? I have slide mailers, but they only hold a few slides. I will do that if I need to (it would be a whole lot of packaging and labeling though...), but don't want to just send a giant box and have them all broken on the other end. Maybe there's another alternative? I'm sure someone on histonet has done this before :) Thanks in advance for the advice. Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley collette2@llnl.gov From raestask <@t> grics.net Fri Jan 8 12:34:46 2010 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Fri Jan 8 12:34:54 2010 Subject: [Histonet] Poor Nuclear Detail Message-ID: <7E55CBF0CE464831AF72D1D1783F26CF@your4105e587b6> We had a case the other day which showed poor nuclear detail. This was fatty breast which had been fixed overnight before processing. Tissues were reprocessed in hopes of improving, but failed to do so. Does anyone have any ideas what may have caused this artifact. All other cases that day were fine. Rae Ann Staskiewicz From trathborne <@t> somerset-healthcare.com Fri Jan 8 12:38:04 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Jan 8 12:38:09 2010 Subject: [Histonet] shipping slides, elementary question In-Reply-To: Message-ID: If you package the slides back-to-back in that box, they will stay more secure. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Nicole Collette Sent: Friday, January 08, 2010 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shipping slides, elementary question Happy Friday everyone, I have a very basic question about shipping slides (mouse tissue, non-biohaz). I am planning to ship a bunch to a collaborator, on the order of a few hundred. I have black hinged cardboard/wood 100-slide boxes, similar to https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=48452-001&inE=1&highlight=48452-001 will these be sufficient for shipping (provided I ensure they stay closed during transit) to avoid breakage? I have slide mailers, but they only hold a few slides. I will do that if I need to (it would be a whole lot of packaging and labeling though...), but don't want to just send a giant box and have them all broken on the other end. Maybe there's another alternative? I'm sure someone on histonet has done this before :) Thanks in advance for the advice. Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley collette2@llnl.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From stamptrain <@t> yahoo.com Fri Jan 8 13:03:51 2010 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Fri Jan 8 13:03:55 2010 Subject: [Histonet] shipping slides, elementary question In-Reply-To: References: Message-ID: <805248.90853.qm@web55801.mail.re3.yahoo.com> We always packed the boxes with wipe-alls to hold the slides secure when the lid was closed, then taped the boxes closed (but those were plastic boxes).? Strong rubber bands can be used with the cardboard/wood boxes to ensure that the lids do not pop open during shipping.? This was successful during the shipment of literally thousands of slide.? Our only problems occurred when the boxes were not as well packed on their return. Roger Moretz, Ph.D. (ret.) ----- Original Message ---- From: "Rathborne, Toni" To: Nicole Collette ; histonet@lists.utsouthwestern.edu Sent: Fri, January 8, 2010 1:38:04 PM Subject: RE: [Histonet] shipping slides, elementary question If you package the slides back-to-back in that box, they will stay more secure. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Nicole Collette Sent: Friday, January 08, 2010 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shipping slides, elementary question Happy Friday everyone, I have a very basic question about shipping slides (mouse tissue, non-biohaz). I am planning to ship a bunch to a collaborator, on the order of a few hundred. I have black hinged cardboard/wood 100-slide boxes, similar to ? https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=48452-001&inE=1&highlight=48452-001 will these be sufficient for shipping (provided I ensure they stay closed during transit) to avoid breakage? I have slide mailers, but they only hold a few slides. I will do that if I need to (it would be a whole lot of packaging and labeling though...), but don't want to just send a giant box and have them all broken on the other end. Maybe there's another alternative? I'm sure someone on histonet has done this before :) Thanks in advance for the advice. Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley collette2@llnl.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From KPercival <@t> wyeth.com Fri Jan 8 13:30:15 2010 From: KPercival <@t> wyeth.com (Percival Karen) Date: Fri Jan 8 13:30:30 2010 Subject: [Histonet] shipping slides, elementary question In-Reply-To: References: Message-ID: <4B47417702000011001F784F@gv01a67m.gv.us.pri.wyeth.com> Hi Nicole, I've shipped slides many times in the hinged, cardboard slide boxes. If you wrap bubble wrap around them and use extra bubble wrap or "peanuts" in the shipping container so that the boxes don't move around, you should be fine. Good luck, karen Karen Percival, BS, HT Research Scientist II Pfizer Research DSRD 1 Burtt Road G3025 Andover, MA 01810 888-577-1500 x 4058 kpercival @wyeth.com >>> Nicole Collette 1/8/2010 1:28 PM >>> Happy Friday everyone, I have a very basic question about shipping slides (mouse tissue, non-biohaz). I am planning to ship a bunch to a collaborator, on the order of a few hundred. I have black hinged cardboard/wood 100-slide boxes, similar to https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=48452-001&inE=1&highlight=48452-001 will these be sufficient for shipping (provided I ensure they stay closed during transit) to avoid breakage? I have slide mailers, but they only hold a few slides. I will do that if I need to (it would be a whole lot of packaging and labeling though...), but don't want to just send a giant box and have them all broken on the other end. Maybe there's another alternative? I'm sure someone on histonet has done this before :) Thanks in advance for the advice. Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley collette2@llnl.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cdemarinis <@t> SARATOGACARE.ORG Fri Jan 8 13:41:53 2010 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Fri Jan 8 13:42:46 2010 Subject: [Histonet] coding Message-ID: What resources are labs using for pathology professional billing coding and modifier questions to assure the correct CPT codes are applied to surgical and cytology cases? This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. From dmccaig <@t> ckha.on.ca Fri Jan 8 14:21:28 2010 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Jan 8 14:21:33 2010 Subject: [Histonet] cassette labelers with bar codes Message-ID: Can anyone advise me on cassette labelers that print 2D bar codes? Preferences or suggestions what to look for. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. From christina.thurby <@t> bms.com Fri Jan 8 15:14:55 2010 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Fri Jan 8 15:15:03 2010 Subject: [Histonet] Question about special stain controls In-Reply-To: References: Message-ID: I have a question for you all regarding the use of special stain controls (non-IHC). I have many years of experience doing special staining and was trained to always treat the control sections the same of the patient or study sample. Does anyone have any references if it is ever appropriate to treat the positive control sample (shortened incubation period) than the patient/study samples? Thanks! Christina Thurby This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From rjbuesa <@t> yahoo.com Fri Jan 8 15:36:08 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 8 15:36:13 2010 Subject: [Histonet] Question about special stain controls In-Reply-To: Message-ID: <83239.81344.qm@web65709.mail.ac4.yahoo.com> If you process the control in any different way than the case slides, it is no longer an acceptable control for that test. Remember that the control is used to determine if the reaction took place when used simultaneously and in an identical way as the test slides. Ren? J. --- On Fri, 1/8/10, Thurby, Christina wrote: From: Thurby, Christina Subject: [Histonet] Question about special stain controls To: "histonet@lists.utsouthwestern.edu" Date: Friday, January 8, 2010, 4:14 PM I have a question for you all regarding the use of special stain controls (non-IHC).? I have many years of experience doing special staining and was trained to always treat the control sections the same of the patient or study sample.? Does anyone have any references if it is ever appropriate to treat the positive control sample (shortened incubation period) than the patient/study samples? Thanks! Christina Thurby This message (including any attachments) may contain confidential, proprietary, privileged and/or private information.? The information is intended to be for the use of the individual or entity designated above.? If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments.? Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Fri Jan 8 15:44:52 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Jan 8 15:45:03 2010 Subject: [Histonet] RE: cassette labelers with bar codes In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A5573@EXCHMBC2.ad.ah.local> >From What I have seen I would go for the newest Thermo Shandon model. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Friday, January 08, 2010 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labelers with bar codes Can anyone advise me on cassette labelers that print 2D bar codes? Preferences or suggestions what to look for. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Marilyn.A.Weiss <@t> kp.org Fri Jan 8 18:01:04 2010 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Jan 8 18:01:11 2010 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 01/08/2010 and will not return until 01/11/2010. .In my absence please ask for Mary Campbell . If this is urgent you can contact me on my cell phone number 858-472-4266. From ndevans <@t> stanford.edu Fri Jan 8 19:17:31 2010 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Fri Jan 8 19:17:35 2010 Subject: [Histonet] Counterstain for fluorescent tissue Message-ID: <9C33B66A3E2F4094AB7FE0CA93ABF5DD@DellDesktop2> Dear all, I am sorry if this is a bit of a basic question. I would like to observe localization of fluorescent microparticles embedded in mouse skin. I plan to do this simply by cryosectioning skin, mounting the tissue without fixation, and observing under the fluorescence microscope (the particles are added before killing the mouse). However, I would also like to counterstain the tissue without losing the fluorescence of my sample (so I can see similar features as I might using H&E). Can anyone suggest a good way to do this? I would be very grateful for any advice. Many thanks Nick Evans From mshaeffer <@t> cox.net Fri Jan 8 20:22:57 2010 From: mshaeffer <@t> cox.net (Marc & Sandy Shaeffer) Date: Fri Jan 8 20:23:04 2010 Subject: [Histonet] Positive Control Tissue for Ab Message-ID: <18653653.13980.1263003777131.JavaMail.mshaeffer@127.0.0.1> IHCWorld.com has a list of antibodies and the positive control tissue that can be used for each antibody at: http://ihcworld.com/_technical_tips/positive_control_tissue_list.htm. I don't have any affiliation with this site or IHCWorld or antibodies or do I sell control tissue (even though this sounds tempting). I'm just a regular histotech who stumbled upon this website and thought it would be cool to share. V/R Marc Shaeffer Tucson Medical Center Tucson Arizona From jkiernan <@t> uwo.ca Sat Jan 9 00:46:20 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Jan 9 00:46:23 2010 Subject: [Histonet] Counterstain for fluorescent tissue In-Reply-To: <9C33B66A3E2F4094AB7FE0CA93ABF5DD@DellDesktop2> References: <9C33B66A3E2F4094AB7FE0CA93ABF5DD@DellDesktop2> Message-ID: Dear Nick Evans, First: Say who and where you are, and who is in charge of your experiments with mice. Second: Tell your boss to buy two or three histotechnology textbooks (about $50 each) and allow himself and you and all your colleagues 30 mins paid daily reading/lunch time. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicholas David Evans Date: Friday, January 8, 2010 20:18 Subject: [Histonet] Counterstain for fluorescent tissue To: histonet@lists.utsouthwestern.edu > Dear all, > > > > I am sorry if this is a bit of a basic question. I would like to > observelocalization of fluorescent microparticles embedded in > mouse skin. I plan > to do this simply by cryosectioning skin, mounting the tissue without > fixation, and observing under the fluorescence microscope (the > particlesare added before killing the mouse). > > > > However, I would also like to counterstain the tissue without > losing the > fluorescence of my sample (so I can see similar features as I > might using > H&E). Can anyone suggest a good way to do this? I would be very > gratefulfor any advice. > > > > Many thanks > > Nick Evans > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Jan 9 07:09:50 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Jan 9 07:09:56 2010 Subject: AW: [Histonet] Poor Nuclear Detail In-Reply-To: <7E55CBF0CE464831AF72D1D1783F26CF@your4105e587b6> References: <7E55CBF0CE464831AF72D1D1783F26CF@your4105e587b6> Message-ID: One cause for poor nuclear detail is drying out of tissue. We saw this, when we let the tissue cassettes to long without formalin before VIP-processing. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rae Staskiewicz Gesendet: Freitag, 08. J?nner 2010 19:35 An: Histonet Betreff: [Histonet] Poor Nuclear Detail We had a case the other day which showed poor nuclear detail. This was fatty breast which had been fixed overnight before processing. Tissues were reprocessed in hopes of improving, but failed to do so. Does anyone have any ideas what may have caused this artifact. All other cases that day were fine. Rae Ann Staskiewicz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anonwums1 <@t> gmail.com Sat Jan 9 11:41:38 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Sat Jan 9 11:41:44 2010 Subject: [Histonet] Counterstain for fluorescent tissue In-Reply-To: References: <9C33B66A3E2F4094AB7FE0CA93ABF5DD@DellDesktop2> Message-ID: <858249121001090941t7085aa87ub28e4c54c0393d5e@mail.gmail.com> Hi, The most common counterstains for fluorescent work is the nuclear counterstain DAPI, which fluoresces in the UV spectrum. If your microparticles fluoresce in that wavelength, then you obviously can't use that but there are a whole host of other nuclear counterstains that fluoresce in pretty much any part of the spectrum you want (see http://www.cbit.uchc.edu/microscopy_facility/fluorescent_probes.html for the most common). Various companies sell antifade mounting media with DAPI included, which is really easy to use. You section your tissue, add the mounting media right on top of the section, coverslip, and then seal with a sealant (usually run-of-the-mill nailpolish). Now here's the problem. Nuclear counterstains stain nuclei really well, but unlike hematoxylin, you often can't see the cell boundaries that well. Sometimes you're lucky in that your tissue is a bit autofluorescent and you can see the cell boundaries that way, and sometimes you can do fancy microscope and optical tricks such as differential interference contrast (DIC). If neither of these work, people use antibodies to highlight some really abundant protein in your tissue such as cytoskeletal proteins. For your work, there might even be a simpler solution. If your microparticles survive hematoxylin staining, you can often just take a bright field photograph with the blue counterstaining, switch on the fluorescent filters, take photographs of that, and then merge them together using image processing software such as Photoshop. Adam On Sat, Jan 9, 2010 at 12:46 AM, John Kiernan wrote: > Dear Nick Evans, > > First: Say who and where you are, and who is in charge of your experiments > with mice. > > Second: Tell your boss to buy two or three histotechnology textbooks > (about $50 each) and allow himself and you and all your colleagues 30 mins > paid daily reading/lunch time. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: Nicholas David Evans > Date: Friday, January 8, 2010 20:18 > Subject: [Histonet] Counterstain for fluorescent tissue > To: histonet@lists.utsouthwestern.edu > > > Dear all, > > > > > > > > I am sorry if this is a bit of a basic question. I would like to > > observelocalization of fluorescent microparticles embedded in > > mouse skin. I plan > > to do this simply by cryosectioning skin, mounting the tissue without > > fixation, and observing under the fluorescence microscope (the > > particlesare added before killing the mouse). > > > > > > > > However, I would also like to counterstain the tissue without > > losing the > > fluorescence of my sample (so I can see similar features as I > > might using > > H&E). Can anyone suggest a good way to do this? I would be very > > gratefulfor any advice. > > > > > > > > Many thanks > > > > Nick Evans > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ndevans <@t> stanford.edu Sat Jan 9 12:12:13 2010 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Sat Jan 9 12:12:19 2010 Subject: [Histonet] Counterstain for fluorescent tissue In-Reply-To: <453665300.1575121263060364761.JavaMail.root@zm06.stanford.edu> Message-ID: <953456084.1575481263060733823.JavaMail.root@zm06.stanford.edu> Dear Adam, Thanks very much for the very helpful advice. I guess my real question, which you're answered for me, was - is there a histological stain v similar to H&E that isn't fluorescent itself, and which you can use without screwing up the intrinsic fluorescence of your tissue? I'll just stick to fluorescent counterstains as I had originally planned. Sorry to offend you John. I had assumed that histonet was a useful forum to get advice on histology and not somewhere to expect slightly narky, rhetorical responses. Best wishes Nick ----- Original Message ----- From: "Adam ." To: "John Kiernan" Cc: "Nicholas David Evans" , histonet@lists.utsouthwestern.edu Sent: Saturday, 9 January, 2010 09:41:38 GMT -08:00 US/Canada Pacific Subject: Re: [Histonet] Counterstain for fluorescent tissue Hi, The most common counterstains for fluorescent work is the nuclear counterstain DAPI, which fluoresces in the UV spectrum. If your microparticles fluoresce in that wavelength, then you obviously can't use that but there are a whole host of other nuclear counterstains that fluoresce in pretty much any part of the spectrum you want (see http://www.cbit.uchc.edu/microscopy_facility/fluorescent_probes.html for the most common). Various companies sell antifade mounting media with DAPI included, which is really easy to use. You section your tissue, add the mounting media right on top of the section, coverslip, and then seal with a sealant (usually run-of-the-mill nailpolish). Now here's the problem. Nuclear counterstains stain nuclei really well, but unlike hematoxylin, you often can't see the cell boundaries that well. Sometimes you're lucky in that your tissue is a bit autofluorescent and you can see the cell boundaries that way, and sometimes you can do fancy microscope and optical tricks such as differential interference contrast (DIC). If neither of these work, people use antibodies to highlight some really abundant protein in your tissue such as cytoskeletal proteins. For your work, there might even be a simpler solution. If your microparticles survive hematoxylin staining, you can often just take a bright field photograph with the blue counterstaining, switch on the fluorescent filters, take photographs of that, and then merge them together using image processing software such as Photoshop. Adam On Sat, Jan 9, 2010 at 12:46 AM, John Kiernan < jkiernan@uwo.ca > wrote: Dear Nick Evans, First: Say who and where you are, and who is in charge of your experiments with mice. Second: Tell your boss to buy two or three histotechnology textbooks (about $50 each) and allow himself and you and all your colleagues 30 mins paid daily reading/lunch time. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicholas David Evans < ndevans@stanford.edu > Date: Friday, January 8, 2010 20:18 Subject: [Histonet] Counterstain for fluorescent tissue To: histonet@lists.utsouthwestern.edu > Dear all, > > > > I am sorry if this is a bit of a basic question. I would like to > observelocalization of fluorescent microparticles embedded in > mouse skin. I plan > to do this simply by cryosectioning skin, mounting the tissue without > fixation, and observing under the fluorescence microscope (the > particlesare added before killing the mouse). > > > > However, I would also like to counterstain the tissue without > losing the > fluorescence of my sample (so I can see similar features as I > might using > H&E). Can anyone suggest a good way to do this? I would be very > gratefulfor any advice. > > > > Many thanks > > Nick Evans > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Jan 9 12:19:25 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Jan 9 12:19:28 2010 Subject: [Histonet] Re: Poor Nuclear Detail Message-ID: Rae Ann Staskiewicz (whrere?) asks: >>We had a case the other day which showed poor nuclear detail. This was fatty breast which had been fixed overnight before processing. Tissues were reprocessed in hopes of improving, but failed to do so. Does anyone have any ideas what may have caused this artifact? All other cases that day were fine.<< It would help to see the sections - do you have any way to post photomicrographs? Let me generalize a bit here - I do some work at a lab that has had a persistent nuclear-bubble artifact for many years. None of their current histotechs ever looks at a slide, and the pathologist has no input. How would you begin to analyze this problem and try to determine its cause? Fixation is in neutral buffered formalin. Fixation time seems to make absolutely no difference, nor is there a difference between small biopsy specimens and too-large tissue blocks. Tumor tissue seem worst affected, but I suspect that all tissues are equally affected. Bob Richmond Samurai Pathologist Knoxville TN From steverchrdsn312 <@t> gmail.com Sat Jan 9 18:09:19 2010 From: steverchrdsn312 <@t> gmail.com (Steve Richardson) Date: Sat Jan 9 18:09:22 2010 Subject: [Histonet] Block and Slide Storage Survey Request Message-ID: <9aa83c741001091609m698c8dv5eb00a6f61f4cf97@mail.gmail.com> Dear Histonet; my name is Steve Richardson and I am looking for your help. I have read much in the archives regarding issues with block and slide storage. I am looking to asses the viability of a small business dedicated to histology block and slide storage. I would like to gather some more data regarding the current block and slide storage practices of labs. If you are interested in taking a survey to assess your practice and needs please respond privately with Yes. Thank you in advance for your help. From tifei <@t> foxmail.com Sat Jan 9 21:48:10 2010 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Sat Jan 9 22:18:48 2010 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIENvdW50ZXJzdGFpbiBmb3IgZmx1b3Jlc2NlbnQgdGlzc3Vl?= References: <953456084.1575481263060733823.JavaMail.root@zm06.stanford.edu> Message-ID: <201001101148093428876@foxmail.com> How about fluorescent nissl? 2010-01-10 TF ???? Nicholas David Evans ????? 2010-01-10 02:16:57 ???? Adam . ??? histonet ??? Re: [Histonet] Counterstain for fluorescent tissue Dear Adam, Thanks very much for the very helpful advice. I guess my real question, which you're answered for me, was - is there a histological stain v similar to H&E that isn't fluorescent itself, and which you can use without screwing up the intrinsic fluorescence of your tissue? I'll just stick to fluorescent counterstains as I had originally planned. Sorry to offend you John. I had assumed that histonet was a useful forum to get advice on histology and not somewhere to expect slightly narky, rhetorical responses. Best wishes Nick ----- Original Message ----- From: "Adam ." To: "John Kiernan" Cc: "Nicholas David Evans" , histonet@lists.utsouthwestern.edu Sent: Saturday, 9 January, 2010 09:41:38 GMT -08:00 US/Canada Pacific Subject: Re: [Histonet] Counterstain for fluorescent tissue Hi, The most common counterstains for fluorescent work is the nuclear counterstain DAPI, which fluoresces in the UV spectrum. If your microparticles fluoresce in that wavelength, then you obviously can't use that but there are a whole host of other nuclear counterstains that fluoresce in pretty much any part of the spectrum you want (see http://www.cbit.uchc.edu/microscopy_facility/fluorescent_probes.html for the most common). Various companies sell antifade mounting media with DAPI included, which is really easy to use. You section your tissue, add the mounting media right on top of the section, coverslip, and then seal with a sealant (usually run-of-the-mill nailpolish). Now here's the problem. Nuclear counterstains stain nuclei really well, but unlike hematoxylin, you often can't see the cell boundaries that well. Sometimes you're lucky in that your tissue is a bit autofluorescent and you can see the cell boundaries that way, and sometimes you can do fancy microscope and optical tricks such as differential interference contrast (DIC). If neither of these work, people use antibodies to highlight some really abundant protein in your tissue such as cytoskeletal proteins. For your work, there might even be a simpler solution. If your microparticles survive hematoxylin staining, you can often just take a bright field photograph with the blue counterstaining, switch on the fluorescent filters, take photographs of that, and then merge them together using image processing software such as Photoshop. Adam On Sat, Jan 9, 2010 at 12:46 AM, John Kiernan < jkiernan@uwo.ca > wrote: Dear Nick Evans, First: Say who and where you are, and who is in charge of your experiments with mice. Second: Tell your boss to buy two or three histotechnology textbooks (about $50 each) and allow himself and you and all your colleagues 30 mins paid daily reading/lunch time. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicholas David Evans < ndevans@stanford.edu > Date: Friday, January 8, 2010 20:18 Subject: [Histonet] Counterstain for fluorescent tissue To: histonet@lists.utsouthwestern.edu > Dear all, > > > > I am sorry if this is a bit of a basic question. I would like to > observelocalization of fluorescent microparticles embedded in > mouse skin. I plan > to do this simply by cryosectioning skin, mounting the tissue without > fixation, and observing under the fluorescence microscope (the > particlesare added before killing the mouse). > > > > However, I would also like to counterstain the tissue without > losing the > fluorescence of my sample (so I can see similar features as I > might using > H&E). Can anyone suggest a good way to do this? I would be very > gratefulfor any advice. > > > > Many thanks > > Nick Evans > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcbook <@t> gmail.com Sun Jan 10 06:25:11 2010 From: jcbook <@t> gmail.com (J C) Date: Sun Jan 10 06:25:16 2010 Subject: [Histonet] Re: Histonet Digest, Vol 74, Issue 8 In-Reply-To: <4b48c46b.2308c00a.3d98.3743SMTPIN_ADDED@mx.google.com> References: <4b48c46b.2308c00a.3d98.3743SMTPIN_ADDED@mx.google.com> Message-ID: <52cb0f131001100425s4c4b73eh3cd020578e1f30f@mail.gmail.com> Dear Histonetters! My question is about Polyester Wax. I know that there is Polyester Wax, with melt temperature 37C. I know where I can buy it. But may be you know somebody that has work with it and can recommend if it is good for work. Thanks, Julie From pruegg <@t> ihctech.net Sun Jan 10 11:42:11 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Jan 10 11:42:47 2010 Subject: [Histonet] testing my new computer Message-ID: <3A85BA6DE84B47EAA1AD1F2E3F59CD9B@medusa> testing my new computer From kc <@t> ka-recruiting.com Sun Jan 10 16:05:41 2010 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Sun Jan 10 16:05:48 2010 Subject: [Histonet] Histology Jobs! Message-ID: <727273020.1263161141077.JavaMail.cfservice@webserver54> Dear Histonet Subscribers, I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. Below is a list of some of the other Histology opportunities we are currently working on. Histotechs/Cytotechs: Connecticut- Histology Operations Manager Southern CA - Histology Supervisor New York City - Surgical Pathology and Histology Supervisor New York City - Histotech 3rd shift Las Vegas, NV - Histotech 3rd shift Central Georgia - Histotech 1st shift Oklahoma - Histotech 1st shift (with opportunity to be promoted to supervisor) Long Island, NY - Cytotech New York City - Cytotech Pennsylvania - Cytology Supervisor Connecticut ? Cytotech If you're interested in learning more about any of these opportunities then please email me a resume and let me know how best to get in touch with you. If none of these are a fit please let me know what you'd be interested in and where you're looking so I can tailor a search for you. With the New Year upon us many of our clients have fresh hiring budgets and will be looking to add people over the next several months. We work on positions at all levels and cover the entire US. To view some additional opportunities please visit our website at www.ka-recruiting.com . Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From histotechkate <@t> gmail.com Sun Jan 10 19:50:04 2010 From: histotechkate <@t> gmail.com (Kate Blanks) Date: Sun Jan 10 19:50:09 2010 Subject: [Histonet] ASCP Certified HT moving to Florida Message-ID: <78df7fdb1001101750n564e06d2ue1ba2ea36465e300@mail.gmail.com> Hello fellow Histonetters! I have read this list for a while now but never had an opportunity to post to it. Now, the time has come to get some info. I am an ASCP certified Histotechnician (HT) with 7 years experience. I also have a NYS license. However, now I am moving to Florida - the Orlando area. I've been trying to find a job for a while now but have been unsuccessful. I am eligible for my Florida license as either a Technician or Technologist with some CE courses. Does anyone on this list have any info about where I should look for employment? Recruiters, do you have any opportunities in the Orlando area that need filling? Thanks all, Kate From jkiernan <@t> uwo.ca Mon Jan 11 00:38:00 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Jan 11 00:38:06 2010 Subject: [Histonet] Counterstain for fluorescent tissue Message-ID: Neutral red (CI 50040) is an excellent fluorescent Nissl stain: 0.002%, in water, for 5 minutes; dehydrate, clear, and mount in DPX or another non-fluorescent resinous medium. With excitation by either near-UV or blue light (range 325-500nm) the Nissl substance and nuclei fluoresce yellow-orange. Reference: Allen, D. T. & Kiernan, J. A. 1994. Permeation of proteins from the blood into peripheral nerves and ganglia. Neuroscience 59(3):755-764. We used this very dilute neutral red as a fluorescent counterstain for paraffin and cryostat sections of formaldehyde-fixed tissues from rats that had received iv injections of rhodamine-labelled albumin (green excitation, orange-red emission, localization mostly extracellular). Franz Nissl was a man, not a granule, substance or stain, so we should give his surname its capital N. Check out http://www.whonamedit.com. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: TF Date: Saturday, January 9, 2010 23:19 > How about fluorescent nissl? >2010-01-10 > TF > ???? Nicholas David Evans > ????? 2010-01-10 02:16:57 > ???? Adam . > ??? histonet > ??? Re: [Histonet] Counterstain for fluorescent tissue > > Dear Adam, > Thanks very much for the very helpful advice. I guess my real > question, which you're answered for me, was - is there a > histological stain v similar to H&E that isn't fluorescent > itself, and which you can use without screwing up the intrinsic > fluorescence of your tissue? I'll just stick to fluorescent > counterstains as I had originally planned. > Sorry to offend you John. I had assumed that histonet was a > useful forum to get advice on histology and not somewhere to > expect slightly narky, rhetorical responses. > Best wishes > Nick > ----- Original Message ----- > From: "Adam ." > To: "John Kiernan" > Cc: "Nicholas David Evans" , > histonet@lists.utsouthwestern.eduSent: Saturday, 9 January, 2010 > 09:41:38 GMT -08:00 US/Canada Pacific > Subject: Re: [Histonet] Counterstain for fluorescent tissue > Hi, > The most common counterstains for fluorescent work is the > nuclear counterstain DAPI, which fluoresces in the UV spectrum. > If your microparticles fluoresce in that wavelength, then you > obviously can't use that but there are a whole host of other > nuclear counterstains that fluoresce in pretty much any part of > the spectrum you want (see > http://www.cbit.uchc.edu/microscopy_facility/fluorescent_probes.html for the most common). Various companies sell antifade mounting media with DAPI included, which is really easy to use. You section your tissue, add the mounting media right on top of the section, coverslip, and then seal with a sealant (usually run-of-the-mill nailpolish). > Now here's the problem. Nuclear counterstains stain nuclei > really well, but unlike hematoxylin, you often can't see the > cell boundaries that well. Sometimes you're lucky in that your > tissue is a bit autofluorescent and you can see the cell > boundaries that way, and sometimes you can do fancy microscope > and optical tricks such as differential interference contrast > (DIC). If neither of these work, people use antibodies to > highlight some really abundant protein in your tissue such as > cytoskeletal proteins. > For your work, there might even be a simpler solution. If your > microparticles survive hematoxylin staining, you can often just > take a bright field photograph with the blue counterstaining, > switch on the fluorescent filters, take photographs of that, and > then merge them together using image processing software such as > Photoshop. > Adam > On Sat, Jan 9, 2010 at 12:46 AM, John Kiernan < > jkiernan@uwo.ca > wrote: > Dear Nick Evans, > First: Say who and where you are, and who is in charge of your > experiments with mice. > Second: Tell your boss to buy two or three histotechnology > textbooks (about $50 each) and allow himself and you and all > your colleagues 30 mins paid daily reading/lunch time. > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: Nicholas David Evans < ndevans@stanford.edu > > Date: Friday, January 8, 2010 20:18 > Subject: [Histonet] Counterstain for fluorescent tissue > To: histonet@lists.utsouthwestern.edu > > Dear all, > > > > > > > > I am sorry if this is a bit of a basic question. I would like > to > > observelocalization of fluorescent microparticles embedded in > > mouse skin. I plan > > to do this simply by cryosectioning skin, mounting the tissue > without > > fixation, and observing under the fluorescence microscope (the > > particlesare added before killing the mouse). > > > > > > > > However, I would also like to counterstain the tissue without > > losing the > > fluorescence of my sample (so I can see similar features as I > > might using > > H&E). Can anyone suggest a good way to do this? I would be > very > > gratefulfor any advice. > > > > > > > > Many thanks > > > > Nick Evans > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Mon Jan 11 10:14:54 2010 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Mon Jan 11 10:14:58 2010 Subject: [Histonet] Used microtome Message-ID: <310388.34347.qm@web23302.mail.ird.yahoo.com> Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi ================================================= In ricordo di "Nice": "E Argo, che aveva visto Odisseo dopo vent?anni, fu preso dal Fato della nera morte." Omero, Odissea, XVII, 290-327 From max_histo_00 <@t> yahoo.it Mon Jan 11 10:14:54 2010 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Mon Jan 11 10:14:59 2010 Subject: [Histonet] Used microtome Message-ID: <310388.34347.qm@web23302.mail.ird.yahoo.com> Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi ================================================= In ricordo di "Nice": "E Argo, che aveva visto Odisseo dopo vent?anni, fu preso dal Fato della nera morte." Omero, Odissea, XVII, 290-327 From shive003 <@t> umn.edu Mon Jan 11 11:07:11 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Jan 11 11:07:16 2010 Subject: [Histonet] shipping slides, elementary question References: Message-ID: <61C385E29BAA437388E191A897CF4785@auxs.umn.edu> I pack slides in 100-slide plastic boxes, with gauze sponges laid inside the boxes on top of slide edges to ensure that they don't jiggle around inside the box. Boxes are taped shut, then packed inside a cardboard box with plenty of room for packing peanuts or bubble wrap. Never have had a problem. Jan Shivers ----- Original Message ----- From: "Nicole Collette" To: Sent: Friday, January 08, 2010 12:28 PM Subject: [Histonet] shipping slides, elementary question > Happy Friday everyone, > > I have a very basic question about shipping slides (mouse tissue, > non-biohaz). I am planning to ship a bunch to a collaborator, on the order > of a few hundred. I have black hinged cardboard/wood 100-slide boxes, > similar to > > > https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=48452-001&inE=1&highlight=48452-001 > > will these be sufficient for shipping (provided I ensure they stay closed > during transit) to avoid breakage? I have slide mailers, but they only > hold a few slides. I will do that if I need to (it would be a whole lot of > packaging and labeling though...), but don't want to just send a giant box > and have them all broken on the other end. Maybe there's another > alternative? I'm sure someone on histonet has done this before :) > > Thanks in advance for the advice. > > Sincerely, > Nicole Collette > Lawrence Livermore National Lab/ UC Berkeley > collette2@llnl.gov > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From llewllew <@t> shaw.ca Mon Jan 11 11:40:38 2010 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Jan 11 11:40:56 2010 Subject: [Histonet] Used microtome In-Reply-To: <310388.34347.qm@web23302.mail.ird.yahoo.com> References: <310388.34347.qm@web23302.mail.ird.yahoo.com> Message-ID: <5D03E599BFDA4CEDAD819549572E002F@BryanPC> Try looking for "microtome" on e-bay. There is a company there that sells a Cambridge rocker for a very reasonable price. Bryan Llewellyn ----- Original Message ----- From: "Massimo" To: Cc: Sent: Monday, January 11, 2010 8:14 AM Subject: [Histonet] Used microtome Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi ================================================= In ricordo di "Nice": "E Argo, che aveva visto Odisseo dopo vent?anni, fu preso dal Fato della nera morte." Omero, Odissea, XVII, 290-327 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwfolsom <@t> rgbio.com Mon Jan 11 11:48:02 2010 From: mwfolsom <@t> rgbio.com (Michael Folsom) Date: Mon Jan 11 11:49:24 2010 Subject: [Histonet] Used microtome In-Reply-To: <310388.34347.qm@web23302.mail.ird.yahoo.com> References: <310388.34347.qm@web23302.mail.ird.yahoo.com> Message-ID: <1263232082.6973.17.camel@chico.322tulane.org> Hi: There are a couple of sources besides the makes and vendors but as always it is "buyer beware". eBay - you can go to www.ebay.com and do a search for microtome, right now they have several for sale. The good thing about eBay is the shipping is often reasonable and you can get a good deal. The bad news is that things can be way overpriced and the sellers usually don't know what they are selling and expensive parts can be missing so you really need to know what you are doing before you buy. GoIndustry - DoveBid at http://www.go-dove.com/default.asp This is a very different deal than eBay. Here you are buying products that are surplus when a company closes or gets rid of excess equipment. Please note microtomes are used in ares outside of BioMedical. For example companies that fabricate plastic often use microtomes for testing purposes so you can find them in "unexpected places". Problem is that with DoveBid shipping is totally on you - unless you are near the auction site you will need to pay a shipper to pick the item up, pack it up and ship it to you. This can easily cost $300 for a microtome so you need to know what your are doing plus you pay a buyer's premium which can range for 16% to 18%. I find equipment on DoveBid is often newer and looks to be in better shape than eBay but it costs lots more. Right now DoveBid lists 5 different microtomes: Shandon Finesse ME Leica RM2135 Biocut 2035 Reicheit-Jung 2050 Leica RM2155 Leica RM2025 My advice is to take your time and know what you are doing - Good luck in your search. Mike Rio Grande Biological On Mon, 2010-01-11 at 16:14 +0000, Massimo wrote: > Hi all, > > I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. > Please can anyone give me useful directions where to find it? > > Thank you all for you help, > > Massimo Tosi > > ================================================= > > In ricordo di "Nice": > > "E Argo, che aveva visto Odisseo dopo vent?anni, > > fu preso dal Fato della nera morte." > > Omero, Odissea, XVII, 290-327 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Cline <@t> wchsys.org Mon Jan 11 12:15:49 2010 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Mon Jan 11 12:17:02 2010 Subject: [Histonet] RE: getting rid of surplus equipment-Source? In-Reply-To: <29A3CB81288E6F4BA2C9B3C8015A9A130102DEC4@MD1EV002.medimmune.com> References: <29A3CB81288E6F4BA2C9B3C8015A9A130102DEC4@MD1EV002.medimmune.com> Message-ID: Belair Instrument Company, 800-783-9424 They have bought most of my old equipment at a fair price. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph [MadaryJ@MedImmune.com] Sent: Thursday, January 07, 2010 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] getting rid of surplus equipment-Source? Does anyone have a good source to get rid of equipment that is still good and maybe our lab can make a few bucks in the process? The last time I did this I was told the processor and stainer I gave away would be going to some charity and of course it did not I found out later. These items are off the books, maybe in need of a small repair. We have no room and the equipment is just been depreciated such that if we can get a credit or something for our lab as a trade in. Specifically we have an RMS microtome in perfect condition used for 6 months and put away. The other is a Leica Embedder that does everyting but dispense paraffin. We were using a separate paraffin dispenser and it was fine, but finally got another embedder. Anyway, vendors welcome. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From rjbuesa <@t> yahoo.com Mon Jan 11 12:23:58 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 11 12:24:02 2010 Subject: [Histonet] Used microtome In-Reply-To: <310388.34347.qm@web23302.mail.ird.yahoo.com> Message-ID: <49987.92365.qm@web65710.mail.ac4.yahoo.com> Try looking in e-Bay. They always have some. Ren? J. --- On Mon, 1/11/10, Massimo wrote: From: Massimo Subject: [Histonet] Used microtome To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 11:14 AM Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi ================================================= In ricordo di "Nice": "E Argo, che aveva visto Odisseo dopo vent?anni, fu preso dal Fato della nera morte." Omero, Odissea, XVII, 290-327 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jan 11 12:23:58 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 11 12:24:05 2010 Subject: [Histonet] Used microtome In-Reply-To: <310388.34347.qm@web23302.mail.ird.yahoo.com> Message-ID: <49987.92365.qm@web65710.mail.ac4.yahoo.com> Try looking in e-Bay. They always have some. Ren? J. --- On Mon, 1/11/10, Massimo wrote: From: Massimo Subject: [Histonet] Used microtome To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 11:14 AM Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi ================================================= In ricordo di "Nice": "E Argo, che aveva visto Odisseo dopo vent?anni, fu preso dal Fato della nera morte." Omero, Odissea, XVII, 290-327 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Mon Jan 11 12:24:12 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Mon Jan 11 12:24:21 2010 Subject: [Histonet] Paraffin Disposal Message-ID: <1872B4A455B7974391609AD8034C79FC8BD687@LBEXCH01.hchd.local> Happy New Year to all. How are you all disposing the paraffin that comes off of the tissue processor? Do you consider it as biohazard waste and disposing it as such or are you putting it into the regular trash disposal? I have been asked why I am disposing it the way I do. I consider it as biohazard waste. Your help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From mucram11 <@t> comcast.net Mon Jan 11 12:32:27 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Jan 11 12:32:31 2010 Subject: [Histonet] Used microtome In-Reply-To: <310388.34347.qm@web23302.mail.ird.yahoo.com> Message-ID: <228041878.133331263234747923.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Hi, I have seen several answer suggesting eBay.? Before you go there you need to know what kind of kinives you be using so you get teh correct knife blade holder.? Are you using disposable knives?? If so are they high or low profile? Are you planning on sharpening solid knives?? What kind of block holder do you need?? Are you embedding in cassettes or with forms?? All of these questions need to be answered before you buy or you could end up with something you can not use or is unusable in your hands.? If you know all these answers eBay could be a good way to go.? I would try to talk to some of the used equipment people and be sure you are going in the correct direction. Pam Marcum UAMS ----- Original Message ----- From: "Massimo" To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Sent: Monday, January 11, 2010 10:14:54 AM GMT -06:00 US/Canada Central Subject: [Histonet] Used microtome Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi ================================================= In ricordo di "Nice": "E Argo, che aveva visto Odisseo dopo vent?anni, fu preso dal Fato della nera morte." Omero, Odissea, XVII, 290-327 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Mon Jan 11 12:42:47 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Jan 11 12:44:53 2010 Subject: [Histonet] Re: Counterstain for fluorescent tissues Message-ID: John, would the neutral red technique work the same with unfixed tissues? The fluorophore Nick uses might not fluoresce after formalin fixation. I suspect he would also need to be sure it's stable after mounting with DPX or other resinous medium. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO Neutral red (CI 50040) is an excellent fluorescent Nissl stain: 0.002%, in water, for 5 minutes; dehydrate, clear, and mount in DPX or another non-fluorescent resinous medium. With excitation by either near-UV or blue light (range 325-500nm) the Nissl substance and nuclei fluoresce yellow-orange. Reference: Allen, D. T. & Kiernan, J. A. 1994. Permeation of proteins from the blood into peripheral nerves and ganglia. Neuroscience 59(3):755-764. We used this very dilute neutral red as a fluorescent counterstain for paraffin and cryostat sections of formaldehyde-fixed tissues from rats that had received iv injections of rhodamine-labelled albumin (green excitation, orange-red emission, localization mostly extracellular). Franz Nissl was a man, not a granule, substance or stain, so we should give his surname its capital N. Check out http://www.whonamedit.com. John Kiernan Anatomy, UWO London, Canada = = = From flnails <@t> texaschildrens.org Mon Jan 11 12:53:19 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon Jan 11 12:53:29 2010 Subject: [Histonet] RE: Paraffin Disposal In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD687@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC8BD687@LBEXCH01.hchd.local> Message-ID: Allison we use tissue prep paraffin and it comes in cardboard containers, we save the empty containers to pour the waste paraffin in. Once it solidifies we dump it in the biohazardous container to be disposed of. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, January 11, 2010 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Disposal Happy New Year to all. How are you all disposing the paraffin that comes off of the tissue processor? Do you consider it as biohazard waste and disposing it as such or are you putting it into the regular trash disposal? I have been asked why I am disposing it the way I do. I consider it as biohazard waste. Your help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From kcai <@t> prosci-inc.com Mon Jan 11 12:54:09 2010 From: kcai <@t> prosci-inc.com (Karen Cai) Date: Mon Jan 11 12:54:12 2010 Subject: [Histonet] flattening tissue surface In-Reply-To: Message-ID: <000a01ca92ef$76278160$9201a8c0@mic> Hi, I need help to prepare some paraffin tissue slides for confocal microscope evaluation. We need the tissue surface very flattened, no wrinkle in order that the microscope can focus the whole section evenly. Anyone has suggestions? Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com From kcai <@t> prosci-inc.com Mon Jan 11 13:00:48 2010 From: kcai <@t> prosci-inc.com (Karen Cai) Date: Mon Jan 11 13:00:50 2010 Subject: [Histonet] Eliminating the edge effect in IHC/IF Message-ID: <000b01ca92f0$63a451c0$9201a8c0@mic> Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com From cbrya <@t> lexclin.com Mon Jan 11 13:10:35 2010 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Mon Jan 11 13:10:39 2010 Subject: [Histonet] Podoplanin D2-40 Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF013BBC4FF0@EXCHANGESB> I am using Podoplanin, D2-40 on cytology cases. We use a mesothelioma tissue for the control and the control performs appropriately, but our cell blocks are staining very faintly. We stain on the Ventana Benchmark using the following protocol: Depar, standard CC1, antibody for 32 min, amplify selected, counterstain, and post counterstain. I am thinking the standard CC1 may be too harsh for the cell blocks and maybe cutting it back to mild treatment may help. Any other suggestions to boost the staining intensity of the podoplanin on the cell blocks? Thank you in advance for your thoughts and input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From histonet.nospam <@t> vneubert.com Mon Jan 11 13:47:45 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Mon Jan 11 13:55:35 2010 Subject: [Histonet] Eliminating the edge effect in IHC/IF In-Reply-To: <000b01ca92f0$63a451c0$9201a8c0@mic> References: <000b01ca92f0$63a451c0$9201a8c0@mic> Message-ID: <4B4B8061.9020901@vneubert.com> Is it just unevenly stained? Or are your sections unevenly thick!? > Hi, > I have a question for the generous input. When I do the IHC or IF, it > seems very common that the intensity of the edge area of the tissue is > always stronger than the central tissue part. Is it possible to > eliminate this and make the staining evenly distributed around the whole > tissue section? > > Your kind help is greatly appreciated, > > > Thanks in advance, > > Best, > Karen > > Karen Cai > Research Scientist > Prosci Incorporated > (858) 513-2638 x 204 > (858) 513-2692 Fax > www.prosci-inc.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cforster <@t> umn.edu Mon Jan 11 14:00:23 2010 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Jan 11 14:00:27 2010 Subject: [Histonet] Eliminating the edge effect in IHC/IF In-Reply-To: <4B4B8061.9020901@vneubert.com> References: <000b01ca92f0$63a451c0$9201a8c0@mic> <4B4B8061.9020901@vneubert.com> Message-ID: <4B4B8357.9070101@umn.edu> This can also be a fixation issue. The edge fixes faster than the center.... Colleen Forster V. Neubert wrote: > Is it just unevenly stained? Or are your sections unevenly thick!? > > > >> Hi, >> I have a question for the generous input. When I do the IHC or IF, it >> seems very common that the intensity of the edge area of the tissue is >> always stronger than the central tissue part. Is it possible to >> eliminate this and make the staining evenly distributed around the whole >> tissue section? >> >> Your kind help is greatly appreciated, >> >> >> Thanks in advance, >> >> Best, >> Karen >> >> Karen Cai >> Research Scientist >> Prosci Incorporated >> (858) 513-2638 x 204 >> (858) 513-2692 Fax >> www.prosci-inc.com >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Mon Jan 11 15:34:13 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 11 15:34:17 2010 Subject: [Histonet] Paraffin Disposal In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD687@LBEXCH01.hchd.local> Message-ID: <176413.99326.qm@web65714.mail.ac4.yahoo.com> It is incinerated. Ren? J. --- On Mon, 1/11/10, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Paraffin Disposal To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 1:24 PM Happy New Year to all.? How are you all disposing the paraffin that comes off of the tissue processor?? Do you consider it as biohazard waste and disposing it as such or are you putting it into the regular trash disposal?? I have been asked why I am disposing it the way I do. I consider it as biohazard waste.? Your help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jan 11 15:38:39 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 11 15:38:43 2010 Subject: [Histonet] flattening tissue surface In-Reply-To: <000a01ca92ef$76278160$9201a8c0@mic> Message-ID: <137571.91940.qm@web65706.mail.ac4.yahoo.com> Cut very thin (about 2 ?m) and let the sections expand well before taking them from the water bath. After that shake them and put the slides vertical on their smaller side (1 inch) for 15 minutes. Take to the oven at 60?C for 10 minutes and proceed as usual. You could also buy a Kurabo S200?sectioning robot that has?been found?to be specially useful for the type of work you are describing (better than manual sectioning). Ren? J.? --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] flattening tissue surface To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 1:54 PM Hi, I need help to prepare some paraffin tissue slides for confocal microscope evaluation. We need the tissue surface very flattened, no wrinkle in order that the microscope can focus the whole section evenly. Anyone has suggestions? Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jan 11 15:39:42 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 11 15:39:45 2010 Subject: [Histonet] Eliminating the edge effect in IHC/IF In-Reply-To: <000b01ca92f0$63a451c0$9201a8c0@mic> Message-ID: <886476.93238.qm@web65706.mail.ac4.yahoo.com> Usually that is the result of incomplete fixation. Check your fixation protocol. Ren? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ndevans <@t> stanford.edu Mon Jan 11 16:05:23 2010 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Mon Jan 11 16:05:28 2010 Subject: [Histonet] Re: Counterstain for fluorescent tissues In-Reply-To: References: Message-ID: <456AC9DD52EE4AFBB92C3E80537E858E@DellDesktop2> Thanks for the help - I could just mount in hydromatrix or similar and view immediately without dehydration. I will try this and will also use DAPI and prepare an H&E on the adjacent slide to show alongside it. Thanks again for the advice, Nick -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Monday, January 11, 2010 10:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Counterstain for fluorescent tissues John, would the neutral red technique work the same with unfixed tissues? The fluorophore Nick uses might not fluoresce after formalin fixation. I suspect he would also need to be sure it's stable after mounting with DPX or other resinous medium. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO Neutral red (CI 50040) is an excellent fluorescent Nissl stain: 0.002%, in water, for 5 minutes; dehydrate, clear, and mount in DPX or another non-fluorescent resinous medium. With excitation by either near-UV or blue light (range 325-500nm) the Nissl substance and nuclei fluoresce yellow-orange. Reference: Allen, D. T. & Kiernan, J. A. 1994. Permeation of proteins from the blood into peripheral nerves and ganglia. Neuroscience 59(3):755-764. We used this very dilute neutral red as a fluorescent counterstain for paraffin and cryostat sections of formaldehyde-fixed tissues from rats that had received iv injections of rhodamine-labelled albumin (green excitation, orange-red emission, localization mostly extracellular). Franz Nissl was a man, not a granule, substance or stain, so we should give his surname its capital N. Check out http://www.whonamedit.com. John Kiernan Anatomy, UWO London, Canada = = = _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madpaw <@t> comcast.net Mon Jan 11 16:06:51 2010 From: madpaw <@t> comcast.net (madpaw@comcast.net) Date: Mon Jan 11 16:06:53 2010 Subject: [Histonet] cassette bar coding and label printing In-Reply-To: <164383340.9721531263247442825.JavaMail.root@sz0079a.westchester.pa.mail.comcast.net> Message-ID: <1095147821.9723141263247611447.JavaMail.root@sz0079a.westchester.pa.mail.comcast.net> We are looking for a system to 2D barcode our cassettes and then at the histology bench with the aide of a 2D barcode reader print labels for the slides. We would like to utilized our current LIS systems.? My question to histoland "What systems are you currently using?? Do you like it and is it user friendly and cost effective? Do you see a reduction in errors due to the implementation of the system?? Do you see a slow down in production due to the application? Thank you in advance for the replies and information. mad From Rcartun <@t> harthosp.org Mon Jan 11 17:03:11 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Jan 11 17:03:22 2010 Subject: [Histonet] Podoplanin D2-40 In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF013BBC4FF0@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF013BBC4FF0@EXCHANGESB> Message-ID: <4B4B67DF.7400.0077.1@harthosp.org> Are your fluids initially fixed in alcohol before the cell block is prepared? If so, that can reduce immunoreactivity for some proteins. If you can, I would recommend trying to decrease the sensitivity of your antigen retrieval. I am a strong proponent of "Personalized" antigen retrieval (adjusting the retrieval based on the type of specimen, fixation, and the length of fixation). I do not have any experience with Ventana's instruments, but on our Bond Max and Bond III instruments, we can adjust the antigen retrieval very easily (low vs. high pH and the time). Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Carol Bryant 1/11/2010 2:10 PM >>> I am using Podoplanin, D2-40 on cytology cases. We use a mesothelioma tissue for the control and the control performs appropriately, but our cell blocks are staining very faintly. We stain on the Ventana Benchmark using the following protocol: Depar, standard CC1, antibody for 32 min, amplify selected, counterstain, and post counterstain. I am thinking the standard CC1 may be too harsh for the cell blocks and maybe cutting it back to mild treatment may help. Any other suggestions to boost the staining intensity of the podoplanin on the cell blocks? Thank you in advance for your thoughts and input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stephen.Eyres <@t> sanofi-aventis.com Tue Jan 12 10:04:49 2010 From: Stephen.Eyres <@t> sanofi-aventis.com (Stephen.Eyres@sanofi-aventis.com) Date: Tue Jan 12 10:04:55 2010 Subject: [Histonet] Problems with MSB stain Message-ID: <90B6684A9D6DAF468F7A5DC148754E1D01858056@ALPW31.f2.enterprise> Hi, I have just encountered a problem I have never seen before and would welcome opinions as to the cause. I work in pharma R&D and had a request to perform MSBs on some liver slides. The results varied within the run, with a distinct difference in the overall colour of the slides; some appearing blue and others red. The person performing the staining is experienced, and our neqas scores are good. When we investigated the problem the only difference we could identify was that the bluer slides were stained after 40 minutes drying after being cut, whereas the redder slides were cut the day before. This suggests that maybe slides were incompletely dried and that this affected the staining. Comments please. Cheers Steve From melissa.ribeiro <@t> brinegroup.com Tue Jan 12 10:55:44 2010 From: melissa.ribeiro <@t> brinegroup.com (Melissa Ribeiro) Date: Tue Jan 12 10:55:52 2010 Subject: [Histonet] Histotech, Mohs opportunity New Hampshire Message-ID: <43904A2EECEAB54D8A023931049FEA4C21876E@brin-sbs01.brinegroup.local> I am recruiting around a Histotech - Mohs position in Southern New Hampshire. Right on the border with Massachusetts. BRIEF SUMMARY: - Under the general supervision of the Microbiology/Histology supervisor will specifically be working performing Mohs cryotonomy. - This includes but is not limited to specimen receipt, mapping the piece of tissue, cutting and slide preparation of frozen tissue, and staining of prepared slides. - Instrument maintenance and quality control documentation is also required. - Previous Mohs experience desired. CONTACT: Melissa Ribeiro @ (781) 272-3400 ext. 228 or via e-mail at mribeiro@brinegroup.com Melissa Ribeiro Passos Healthcare Division Brine Group Staffing Solutions 20 Mall Road, Suite 225 Burlington, MA 01803 mribeiro@brinegroup.com Ph.? (781) 272-3400 ext. 228 Fax (781) 494-3401 Save a Tree! Consider the environment before you print this e-mail.? Visit our newly redesigned web site at www.brinegroup.com, the intelligent choice for staffing solutions. ? From mpowers <@t> dpspa.com Tue Jan 12 13:34:33 2010 From: mpowers <@t> dpspa.com (Marian Powers) Date: Tue Jan 12 13:34:40 2010 Subject: [Histonet] IHC Message-ID: <5d7de0e61001121134g662d43adr3a7c4cd1b0235854@mail.gmail.com> Hi: We are looking to start N-ras and B-raf for IHC, is anyone running these antibodies on paraffin? If so, what clone and vendor are you using? Thanks in advance! -- Marian L. Powers, HT(ASCP) Manager, Technical Operations Doctors Pathology Services 1253 College Park Drive Dover, DE 19904 302-677-0000 From c.m.vanderloos <@t> amc.uva.nl Tue Jan 12 13:54:35 2010 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Tue Jan 12 13:55:09 2010 Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF Message-ID: <9637c5fe5d7dffc7.4b4ce18b@amc.uva.nl> Hi all,We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy. One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved. As a result the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely, including a rim around the section. However, to be honest, I am sure my explanation is certainly not always appropriate. Anyone else????Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com From MSHERWOOD <@t> PARTNERS.ORG Tue Jan 12 14:28:55 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Jan 12 14:29:01 2010 Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF In-Reply-To: <9637c5fe5d7dffc7.4b4ce18b@amc.uva.nl> References: <9637c5fe5d7dffc7.4b4ce18b@amc.uva.nl> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F19@PHSXMB30.partners.org> Actually, when I read about the problem, my thought was that if you don't circle your tissue with an immunopen or wax pencil, then the reagents (esp. primary antibody)might not cover the tissue evenly as you say. So I tend to agree with you. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Tuesday, January 12, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF Hi all,We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy. One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved. As a result the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely, including a rim around the section. However, to be honest, I am sure my explanation is certainly not always appropriate. Anyone else????Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rjbuesa <@t> yahoo.com Tue Jan 12 14:40:16 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 12 14:40:22 2010 Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF In-Reply-To: <9637c5fe5d7dffc7.4b4ce18b@amc.uva.nl> Message-ID: <661401.32335.qm@web65710.mail.ac4.yahoo.com> Hi Dr. van der Loos: I had also experienced that artifact caused by less than necessary reagents, but that happens?mostly when IHC?is done manually, or when the autostainer delivers less amount than required or programmed. IF we assume that the colleague with the question did the IHC manually, your explanation can be accepted, otherwise, poor fixation is a valid?cause to the problem. Ren? J. --- On Tue, 1/12/10, C.M. van der Loos wrote: From: C.M. van der Loos Subject: RE: Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com, rjbuesa@yahoo.com, cforster@umn.edu Date: Tuesday, January 12, 2010, 2:54 PM Hi all, We also have observed this phenomenon many times. But sorry Colleen and Rene,?I don't believe?that an?fixation issue?is the explanation why the edges are sometimes stronger than the rest. To my opinion this is?a bit too easy.?One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved.?As a result?the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely,?including a rim around the section.?However, to?be honest,?I am sure my?explanation is certainly not always appropriate. Anyone else???? Cheers, Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone:? +31 20 5665631 ? From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com From dellav <@t> musc.edu Tue Jan 12 14:40:55 2010 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Jan 12 14:41:00 2010 Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF In-Reply-To: <9637c5fe5d7dffc7.4b4ce18b@amc.uva.nl> References: <9637c5fe5d7dffc7.4b4ce18b@amc.uva.nl> Message-ID: I think we'll agree that there are different scenarios so that the solution is not a one size fits all. For example, darkened staining around the periphery of needle core biopsies is not uncommon with even tinctorial stains, often thought to be the result of drying of the tissue during the collection of the sample. Years ago I came across an article maintaining that the dark staining on the periphery of needle cores was in fact due to the "trauma" of the needle cutting into the sampled organ. I've since forgotten the author's name and wish I could get my hands on that reference. So I agree with Chris that there doesn't appear to be one simple answer to prevent this artifact and while fixation may contribute in some circumstances it's unlikely to be the remedy for all. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Tuesday, January 12, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF Hi all,We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy. One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved. As a result the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely, including a rim around the section. However, to be honest, I am sure my explanation is certainly not always appropriate. Anyone else????Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 12 14:41:24 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 12 14:41:29 2010 Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F19@PHSXMB30.partners.org> Message-ID: <842909.92777.qm@web65701.mail.ac4.yahoo.com> Again, provided that you are doing IHC manually. Ren? J. --- On Tue, 1/12/10, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF To: "C.M. van der Loos" , histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Date: Tuesday, January 12, 2010, 3:28 PM Actually, when I read about the problem, my thought was that if you don't circle your tissue with an immunopen or wax pencil, then the reagents (esp. primary antibody)might not cover the tissue evenly as you say.? So I tend to agree with you. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Tuesday, January 12, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF Hi all,We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy. One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved. As a result the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely, including a rim around the section. However, to be honest, I am sure my explanation is certainly not always appropriate. Anyone else????Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone:? +31 20 5665631 From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hborgeri <@t> wfubmc.edu Tue Jan 12 15:03:13 2010 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Tue Jan 12 15:04:25 2010 Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF In-Reply-To: <842909.92777.qm@web65701.mail.ac4.yahoo.com> References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F19@PHSXMB30.partners.org> <842909.92777.qm@web65701.mail.ac4.yahoo.com> Message-ID: <9AEEF1FB6254224AA355ED285F8491653EE04680@EXCHVS2.medctr.ad.wfubmc.edu> I do my immuno staining manually using ProbeOn Plus slides which employ the capillary action principle. I always monitor the taking up/whicking off of my solutions for each pair of slides throughout the entire staining process and know that my sections never dry out (I incubate using moist chambers with plenty of fluid). I work at a research/diagnostic medical school facility where our primary focus is on experimental tissues, using standardized and rigid guidelines for fixation: 24 hours at 4 degrees overnight in 4% PF, followed by post fixation in 70% ethanol for a few days. Never see the "edge effect" with these sections. However, I do occasionally see the effect using archival diagnostic samples that were fixed in 10% NBF for a minimum of 48 hours, and probably longer. I have therefore always perceived this effect to be caused by extended, rather than incomplete fixation. Hermina -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 12, 2010 3:41 PM To: C.M. van der Loos; histonet@lists.utsouthwestern.edu; MargaretSherwood Cc: kcai@prosci-inc.com Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF Again, provided that you are doing IHC manually. Ren? J. --- On Tue, 1/12/10, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF To: "C.M. van der Loos" , histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Date: Tuesday, January 12, 2010, 3:28 PM Actually, when I read about the problem, my thought was that if you don't circle your tissue with an immunopen or wax pencil, then the reagents (esp. primary antibody)might not cover the tissue evenly as you say.? So I tend to agree with you. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Tuesday, January 12, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF Hi all,We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy. One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved. As a result the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely, including a rim around the section. However, to be honest, I am sure my explanation is certainly not always appropriate. Anyone else????Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone:? +31 20 5665631 From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathmaster <@t> yahoo.com Tue Jan 12 16:30:46 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Tue Jan 12 16:30:51 2010 Subject: [Histonet] Fw: Waste paraffin and edge effect Message-ID: <13966.65872.qm@web111106.mail.gq1.yahoo.com> --- Hello everyone, ? Our?system's ?hazardous chemical waste consultants changed our method of disposal- we had been solidifying the paraffin in a hood and then red bagging it, ie treating it as regulated medical (biohazard) waste. ? But, due to it's contamination with xylene, the very reason for our disposing of it, the paraffin is really hazardous chemical waste. Believe it or not, EPA regs prohibit most hospitals from treating their chemical waste and the consultants worried that solidifying it under the hood was considered "treating" the waste.? Yeah right!!? ?Anyway, the blocks of solidified paraffin are now dumped in?it's own specially labelled drum and has become a part of our hazardous waste stream, manifested like the xylene and dye waste. I'd be careful about putting hazardous chemicals into?regulated medical waste. ? Interestingly, our system recently inquired if formalin in discarded surgicals needs to be decanted before disposal- many hospitals are doing just that, but most of those, but not all, recycle formalin (yuk!).?Our disposal facility informed us that as long as the containers are plastic, ie are flammamble, they can be incinerated with the formalin and there is no need to decant. ?Glory be!! ? In IHC slides, edge effect, or more intense, sometimes?nonspecific, staining at the periphery of the tissue, can be caused? by more intense fixation of a block at the periphery,?drying out of the edges of a block before fiation, ?and/or by some degree of drying of antibodies and detection chemistry reagents during?staining- this cause is more common in manually stained slides rather than those stained on automated stainers. ? ? Also electrocautery?used during the excision can also cause intense staining at the edges of specimens. ? Jeffrey S. Silverman HT HTL QIHC (ASCP) Southside Hospital- NSLIJ Health System Pathologists' Assistant and Laboratory Safety Officer From annigyg <@t> gmail.com Wed Jan 13 03:15:36 2010 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Jan 13 03:15:42 2010 Subject: [Histonet] Re: Sakura iDent In-Reply-To: References: Message-ID: Hi Denise sadly no resolution also sadly very little response from the histonet - except for Rene - he and i did some troubleshooting to try to see if i had missed anything - we had the same thoughts so ... i am still sitting with the iDent in its box, unused, until someone, somewhere can convince me that the print does not wipe off with xylene - which will be hard to do seeing that i wiped it off myself and saw with my own eyes that xylene removes the ink!! Sakura - if you are reading this - i am not a happy camper Annie 2010/1/12 Denise Van Eaton > Hi Anne, > > We have been checking into cassette printers. Obviously, if we can bring > one in for a demonstration we will but I thought the iDent looked like a > good place to start. I noticed your problem (on the Histonet) with the ink > rubbing off after the xylene. I never saw any responses from the rest of the > Histonetters... did you resolve the problem? Assuming you (or Sakura) did, > what was the trick? > > Thank you in advance for sharing your "fix" for a potential problem. > > Denise Van Eaton HT(ASCP) > Litton Pathology Associates > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From kryan <@t> nfderm.com Wed Jan 13 07:58:54 2010 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Wed Jan 13 07:58:57 2010 Subject: [Histonet] Looking for Florida licensed Histologist Message-ID: North Florida Dermatology Associates located in Jacksonville, Florida is looking for a full time histologist. Must be eligible for Florida State Technologist license. The lab is a small private lab with good benefits and minimal stress. Responsibilities include routine histology and some IHC techniques. If interested, please contact me or our Personnel Director at 904-398-0547, ext. 1106. Kaye Ryan North Florida Dermatology Associates Histology Lab Supervisor (904) 398-0547 Ext. 2004 From cpyse <@t> x-celllab.com Wed Jan 13 08:03:34 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Jan 13 08:05:31 2010 Subject: [Histonet] Re: Sakura iDent In-Reply-To: References: Message-ID: <000001ca9459$32066300$96132900$@com> Anne You might want to try going up the Sakura chain of command. I had trouble with a coverslipper, after contacting the regional manager it was amazing the service it received. Good luck. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Wednesday, January 13, 2010 4:16 AM To: Denise Van Eaton Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Sakura iDent Hi Denise sadly no resolution also sadly very little response from the histonet - except for Rene - he and i did some troubleshooting to try to see if i had missed anything - we had the same thoughts so ... i am still sitting with the iDent in its box, unused, until someone, somewhere can convince me that the print does not wipe off with xylene - which will be hard to do seeing that i wiped it off myself and saw with my own eyes that xylene removes the ink!! Sakura - if you are reading this - i am not a happy camper Annie 2010/1/12 Denise Van Eaton > Hi Anne, > > We have been checking into cassette printers. Obviously, if we can bring > one in for a demonstration we will but I thought the iDent looked like a > good place to start. I noticed your problem (on the Histonet) with the ink > rubbing off after the xylene. I never saw any responses from the rest of the > Histonetters... did you resolve the problem? Assuming you (or Sakura) did, > what was the trick? > > Thank you in advance for sharing your "fix" for a potential problem. > > Denise Van Eaton HT(ASCP) > Litton Pathology Associates > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Jan 13 08:32:19 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jan 13 08:32:26 2010 Subject: AW: [Histonet] Eliminating the edge effect in IHC/IF In-Reply-To: <000b01ca92f0$63a451c0$9201a8c0@mic> References: <000b01ca92f0$63a451c0$9201a8c0@mic> Message-ID: <21FEAA12A3C44D2FBE8EC603949E9CB3@dielangs.at> My favourite for intense specific staining at the edges vs. faint specific staining in the center is poor fixation. I think drying artefacts or crush artefacts would lead rather to unspecific background staining. Gudrun Lang Histolab, Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Karen Cai Gesendet: Montag, 11. J?nner 2010 20:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Eliminating the edge effect in IHC/IF Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjkitten <@t> live.com Wed Jan 13 08:44:03 2010 From: sjkitten <@t> live.com (S R) Date: Wed Jan 13 08:44:07 2010 Subject: [Histonet] Bladder Biopsies Message-ID: Hello Everyone! I have a couple of questions. I work for a urology group. As of right now i only work with prostate bx's. They want to add Bladder bx's into the mix. As of right now i microwave process them. I was wondering if anyone eles does this and if you use a different procedure than you would use for processing the prostate biopsies, and would anyone be willing to share? Thanks in Advanced Sammy _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/196390709/direct/01/ From LRaff <@t> uropartners.com Wed Jan 13 09:09:54 2010 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Wed Jan 13 09:09:58 2010 Subject: [Histonet] Re: Bladder Biopsies Message-ID: We microwave process our bladder biopsies on the same cycle as prostate biopsies. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 From histology <@t> medsurgpath.com Wed Jan 13 09:50:50 2010 From: histology <@t> medsurgpath.com (histology@medsurgpath.com) Date: Wed Jan 13 09:50:54 2010 Subject: [Histonet] Eliminating the edge effect in IHC/IF In-Reply-To: <000b01ca92f0$63a451c0$9201a8c0@mic> References: <000b01ca92f0$63a451c0$9201a8c0@mic> Message-ID: <61410.68.178.72.125.1263397850.squirrel@webmail.integra.net> Hi Karen/Histonet, This is a problem we have been working on for some time with our IHC. We have ruled out fixation as the source of the problem, and we are in Oregon so we don't believe we are having a drying out issue. I have also tried different detection kits with both having this artifact. Recently we were wondering if it could be a problem with our blower. Is it possible that it is blowing too hard, or that the rinses are too hard? We have tried hand staining twice, once with perfect results, once with the same artifact. Any additional advice you can give will be greatly appreciated. Thank you, once again, Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (formerly Cutting Edge Histology) (503)443-2157 > Hi, > I have a question for the generous input. When I do the IHC or IF, it > seems very common that the intensity of the edge area of the tissue is > always stronger than the central tissue part. Is it possible to > eliminate this and make the staining evenly distributed around the whole > tissue section? > > Your kind help is greatly appreciated, > > > Thanks in advance, > > Best, > Karen > > Karen Cai > Research Scientist > Prosci Incorporated > (858) 513-2638 x 204 > (858) 513-2692 Fax > www.prosci-inc.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Alice.Fallak <@t> uhsi.org Wed Jan 13 10:14:18 2010 From: Alice.Fallak <@t> uhsi.org (Fallak, Alice) Date: Wed Jan 13 10:14:22 2010 Subject: [Histonet] Part-time Flex Histologist position Message-ID: We have a part-time/Flex Histologist position open at United Hospital and Medical Center in Kenosha,Wi. Anyone interested can contact our Human Resource Dept. at (262)656-2116. Alice Fallak Histology coordinator United Hosp. & Med. Center From NKlemme <@t> sakuraus.com Wed Jan 13 10:21:12 2010 From: NKlemme <@t> sakuraus.com (Nancy Klemme) Date: Wed Jan 13 10:22:56 2010 Subject: [Histonet] Re: Sakura iDent In-Reply-To: References: Message-ID: <782E3A02C2EB2347BEA6DEA69DC7AB8669D9A4399E@sfamail.SAKURAUS.LOCAL> Dear Annie, I am writing you from California where our Wednesday workday is beginning and you are preparing for your Wednesday to draw to a close. Although the majority of Sakura Finetek products are carried by all Sakura Finetek entities, the iDent is not one that is available from Sakura Finetek USA in North America. If most Histonet subscribers are from the U.S., that may explain the "very little response from the histonet" that you noted. I am sorry about your cassette labeler issues and have forwarded your posting to the gentleman who is in charge of Sakura Finetek Middle East. His name is Mr. Ghaleb Alkhaseb and I am sure he will be in contact with you very soon after reading it. I do not know what Sakura contact information you have been using, but I know that Mr. Alkhaseb will make sure that you will have the most current information for a more direct company contact. I do expect your iDent situation will be corrected and resolved to your satisfaction very soon. >From 12 hours behind you, I offer my kindest regards. Nancy Klemme, HT(ASCP) Edu. Svcs. Dir. - Sakura Finetek USA, Inc. - Torrance, CA 800-725-8723 x7879 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Wednesday, January 13, 2010 1:16 AM To: Denise Van Eaton Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Sakura iDent Hi Denise sadly no resolution also sadly very little response from the histonet - except for Rene - he and i did some troubleshooting to try to see if i had missed anything - we had the same thoughts so ... i am still sitting with the iDent in its box, unused, until someone, somewhere can convince me that the print does not wipe off with xylene - which will be hard to do seeing that i wiped it off myself and saw with my own eyes that xylene removes the ink!! Sakura - if you are reading this - i am not a happy camper Annie 2010/1/12 Denise Van Eaton > Hi Anne, > > We have been checking into cassette printers. Obviously, if we can bring > one in for a demonstration we will but I thought the iDent looked like a > good place to start. I noticed your problem (on the Histonet) with the ink > rubbing off after the xylene. I never saw any responses from the rest of the > Histonetters... did you resolve the problem? Assuming you (or Sakura) did, > what was the trick? > > Thank you in advance for sharing your "fix" for a potential problem. > > Denise Van Eaton HT(ASCP) > Litton Pathology Associates > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Jan 13 10:24:19 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Jan 13 10:24:30 2010 Subject: [Histonet] Problems with MSB stain In-Reply-To: <90B6684A9D6DAF468F7A5DC148754E1D0185805C@ALPW31.f2.enterprise> References: <90B6684A9D6DAF468F7A5DC148754E1D0185805C@ALPW31.f2.enterprise> Message-ID: The unduly blue sections are all from Animals 12, 13 and 14. Anything different about these specimens? The blue and red sections surely must look different under the microscope. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Stephen.Eyres@sanofi-aventis.com Date: Wednesday, January 13, 2010 10:12 Subject: RE: [Histonet] Problems with MSB stain To: jkiernan@uwo.ca > Hi John, > > Many thanks for the rapid response. The slides look fine down the microscope except for a few that macroscopically look blue. However because the MSBs will be sent away for evaluation, we want to send a consistent set of slides. I attach a picture to show the problem. I can also scan a slide if you prefer, but the image size is large. The paper was very helpful. > > Cheers > > Steve > > From: John Kiernan [mailto:jkiernan@uwo.ca] > Sent: Wednesday, January 13, 2010 6:23 AM > To: Eyres, Stephen R&D/GB > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Problems with MSB stain > > What do the slides look like under the microscope? In a normal liver there shouldn't be much collagen (blue) and there should be plenty of cytoplasm (red). If the preceding nuclear stain was too strong, that could put unwanted bluish shades in the cytoplasm. > > Trichrome methods aren't at their best after formaldehyde fixation; the colours can be improved by soaking the hydrated sections in saturated aqueous picric acid for 30 min or so at 55-60C before staining. Bouin's solution is also used for this purpose, and it is said that the same effect can be achieved with citrate buffer pH4 or with 1% iodine in 2% aqueous KI. See Yu Y, Chapman CM (2003) Masson trichrome stain: postfixation substitutes. J. Histotechnol. 26: 131-134. I've not yet tried these alternatives to picric acid; has anyone else? > > Lendrum's 1962 paper, which includes colour photos, includes many technical tips. More than 40 years ago a med lab technician in Birmingham, UK, suggested this method to me as a way to impart different colours to gliosis (red) and collagenous scarring (blue) in sections of the brains of frogs and other animals in which I'd made surgical lesions dorsal to the optic chiasma. The MSB method showed these changes very well. I recall that we had to buy about 500 grams of brilliant crystal scarlet 6R because it was then cheaper as an an article of commerce than as a bioogical stain. > > I have appended a PDF of Lendrum et al 1962. This won't gwt through to all Histonetters, but it might make it to Stephen.Eyres@sanofi-aventis.com. > > > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: Stephen.Eyres@sanofi-aventis.com > Date: Tuesday, January 12, 2010 11:06 > Subject: [Histonet] Problems with MSB stain > To: histonet@lists.utsouthwestern.edu > > > Hi, > > > > I have just encountered a problem I have never seen before and would > > welcome opinions as to the cause. I work in pharma R&D and had a > > requestto perform MSBs on some liver slides. The results varied > > within the run, > > with a distinct difference in the overall colour of the > > slides; some > > appearing blue and others red. The person performing the > > staining is > > experienced, and our neqas scores are good. When we investigated the > > problem the only difference we could identify was that the bluer > > slideswere stained after 40 minutes drying after being > > cut, whereas the > > redder slides were cut the day before. This suggests that maybe slides > > were incompletely dried and that this affected the staining. Comments > > please. > > > > Cheers > > > > Steve > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Wed Jan 13 10:50:18 2010 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Wed Jan 13 10:50:24 2010 Subject: [Histonet] (Used microtome) - Thanks ... Message-ID: <939066.56789.qm@web23307.mail.ird.yahoo.com> ... to all those who kindly responded at my email. Best Regards, Massimo Tosi ================================================= In ricordo di "Nice": "E Argo, che aveva visto Odisseo dopo vent?anni, fu preso dal Fato della nera morte." Omero, Odissea, XVII, 290-327 From max_histo_00 <@t> yahoo.it Wed Jan 13 10:50:18 2010 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Wed Jan 13 10:50:25 2010 Subject: [Histonet] (Used microtome) - Thanks ... Message-ID: <939066.56789.qm@web23307.mail.ird.yahoo.com> ... to all those who kindly responded at my email. Best Regards, Massimo Tosi ================================================= In ricordo di "Nice": "E Argo, che aveva visto Odisseo dopo vent?anni, fu preso dal Fato della nera morte." Omero, Odissea, XVII, 290-327 From gu.lang <@t> gmx.at Wed Jan 13 10:58:18 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jan 13 10:58:24 2010 Subject: AW: [Histonet] Problems with MSB stain In-Reply-To: <90B6684A9D6DAF468F7A5DC148754E1D01858056@ALPW31.f2.enterprise> References: <90B6684A9D6DAF468F7A5DC148754E1D01858056@ALPW31.f2.enterprise> Message-ID: <76730F9EC40D4109BDEB62A3B9762C60@dielangs.at> Hi Steve, I have noticed that the CAB-stain, which is also a trichrome-stain (like Trichrome Gomori), shows a blue overall appearence after NBF-fixation alone. After re-fixation in Bouin the staining shows normal red staining of liverslides. Re-fixation is like "antigen-retrieval" and opens the right binding sites for the dyes. Did you re-fix your slides in Bouin? Is there a difference in fixation of the specimen? Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Stephen.Eyres@sanofi-aventis.com Gesendet: Dienstag, 12. J?nner 2010 17:05 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Problems with MSB stain Hi, I have just encountered a problem I have never seen before and would welcome opinions as to the cause. I work in pharma R&D and had a request to perform MSBs on some liver slides. The results varied within the run, with a distinct difference in the overall colour of the slides; some appearing blue and others red. The person performing the staining is experienced, and our neqas scores are good. When we investigated the problem the only difference we could identify was that the bluer slides were stained after 40 minutes drying after being cut, whereas the redder slides were cut the day before. This suggests that maybe slides were incompletely dried and that this affected the staining. Comments please. Cheers Steve _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Wed Jan 13 11:25:28 2010 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Jan 13 11:25:35 2010 Subject: [Histonet] Re: Sakura iDent In-Reply-To: <782E3A02C2EB2347BEA6DEA69DC7AB8669D9A4399E@sfamail.SAKURAUS.LOCAL> References: <782E3A02C2EB2347BEA6DEA69DC7AB8669D9A4399E@sfamail.SAKURAUS.LOCAL> Message-ID: Dear Nancy thank you for your email - yes, I know Ghaleb well and have had a working relationship with him for many years here in the middle east. He is indeed trying to extract some sort of resolution from the Sakura European Head office in Zoeterwoude - Ralph, Wim, Chris and Kees-Jan ....my local vendor here in the UAE is excellent and he has witnessed my print rubbing off the cassette face when gently brushed with xylene on gloved finger or a cotton swab. I must just add that it is very strange for me to be battling with a company I have avidly supported and promoted for so many years. I hope that we will resolve this issue and that i will add yet another Sakura workhorse to my already large Sakura stable. kind regards Annie 2010/1/13 Nancy Klemme > Dear Annie, > > I am writing you from California where our Wednesday workday is beginning > and you are preparing for your Wednesday to draw to a close. > > Although the majority of Sakura Finetek products are carried by all Sakura > Finetek entities, the iDent is not one that is available from Sakura Finetek > USA in North America. If most Histonet subscribers are from the U.S., that > may explain the "very little response from the histonet" that you noted. > > I am sorry about your cassette labeler issues and have forwarded your > posting to the gentleman who is in charge of Sakura Finetek Middle East. > His name is Mr. Ghaleb Alkhaseb and I am sure he will be in contact with > you very soon after reading it. I do not know what Sakura contact > information you have been using, but I know that Mr. Alkhaseb will make sure > that you will have the most current information for a more direct company > contact. I do expect your iDent situation will be corrected and resolved to > your satisfaction very soon. > > From 12 hours behind you, I offer my kindest regards. > > Nancy Klemme, HT(ASCP) > Edu. Svcs. Dir. - Sakura Finetek USA, Inc. - Torrance, CA > 800-725-8723 x7879 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van > Binsbergen > Sent: Wednesday, January 13, 2010 1:16 AM > To: Denise Van Eaton > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Sakura iDent > > Hi Denise > sadly no resolution > also sadly very little response from the histonet - except for Rene - he > and > i did some troubleshooting to try to see if i had missed anything - we had > the same thoughts > so ... i am still sitting with the iDent in its box, unused, until someone, > somewhere can convince me that the print does not wipe off with xylene - > which will be hard to do seeing that i wiped it off myself and saw with my > own eyes that xylene removes the ink!! > > Sakura - if you are reading this - i am not a happy camper > > Annie > 2010/1/12 Denise Van Eaton > > > Hi Anne, > > > > We have been checking into cassette printers. Obviously, if we can bring > > one in for a demonstration we will but I thought the iDent looked like a > > good place to start. I noticed your problem (on the Histonet) with the > ink > > rubbing off after the xylene. I never saw any responses from the rest of > the > > Histonetters... did you resolve the problem? Assuming you (or Sakura) > did, > > what was the trick? > > > > Thank you in advance for sharing your "fix" for a potential problem. > > > > Denise Van Eaton HT(ASCP) > > Litton Pathology Associates > > > > > > -- > Anne van Binsbergen (Hope) > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From rsrichmond <@t> gmail.com Wed Jan 13 11:37:54 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jan 13 11:37:57 2010 Subject: [Histonet] Re: Bladder Biopsies Message-ID: In answer to a query about microwave processing of urinary bladder biopsy specimens Lester Raff MD (Uropartners, Winchester IL) notes: >>We microwave process our bladder biopsies on the same cycle as prostate biopsies.<< Good to know this. Do the immunostains require any adjustment - particularly the racemase stain, notorious for difficulty in adjusting to different fixations? Bob Richmond Samurai Pathologist Knoxville TN From turkekul <@t> gmail.com Wed Jan 13 11:56:34 2010 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Wed Jan 13 11:56:38 2010 Subject: [Histonet] IHC edge effect Message-ID: Dear Colleagues, In my opinion the edge effect problem has many different components. In each particular case the reason/reasons for edge effect are different. Sometimes I observe that the staining intensity gradually decreases towards the center of the section. In this case I think this is mainly fixation problem. I had experienced edge effect with manual as well different automated IHC staining systems. Sometimes adjacent sections from he same block give different intensity of edge effect with different antibodies/protocols. Sometimes the edge effect is due to the trauma caused during cutting/crossing the specimen before fixation. Sometimes due to HIER methods or drying of the sections during staining. And different antibodies/IHC protocols have different sensitivities to all of the above phenomena that tortures the tissue/section. All the answers given before to that question are correct. Regards, Mesruh Turkekul Memorial Sloan-Kettering Cancer Center New York, NY 10021 From dimitri.scholz <@t> ucd.ie Wed Jan 13 12:09:53 2010 From: dimitri.scholz <@t> ucd.ie (Dimitri Scholz) Date: Wed Jan 13 12:09:58 2010 Subject: [Histonet] IHC edge effect In-Reply-To: References: Message-ID: <013a01ca947b$9b285380$d178fa80$%scholz@ucd.ie> I would agree with Mesruh: there could be different reasons. Own experience: if you stain large semithin Epon sections with toluidin blue, edges are usually darker stained than the center. At least for this case, we can exclude drying and be sure the different penetration of osmium fixation is the reason. In case of immunolabeling, however, my first move would be to check if the sections are dried, which could cause non-specific binding or even some specific one caused by antigen retrieval (each tissue damage could work as an antigen retrieval!). Regards, Dimitri Dimitri Scholz, PhD, Dr. Sci. Director of Biological Imaging Assotiate Professor Conway Institute University College Dublin (UCD) Belfield, Dublin 4 Ireland Tel: +353-1 716 6736 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mesruh turkekul Sent: 13 January 2010 17:57 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC edge effect Dear Colleagues, In my opinion the edge effect problem has many different components. In each particular case the reason/reasons for edge effect are different. Sometimes I observe that the staining intensity gradually decreases towards the center of the section. In this case I think this is mainly fixation problem. I had experienced edge effect with manual as well different automated IHC staining systems. Sometimes adjacent sections from he same block give different intensity of edge effect with different antibodies/protocols. Sometimes the edge effect is due to the trauma caused during cutting/crossing the specimen before fixation. Sometimes due to HIER methods or drying of the sections during staining. And different antibodies/IHC protocols have different sensitivities to all of the above phenomena that tortures the tissue/section. All the answers given before to that question are correct. Regards, Mesruh Turkekul Memorial Sloan-Kettering Cancer Center New York, NY 10021 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jefthompson <@t> salud.unm.edu Wed Jan 13 13:04:29 2010 From: jefthompson <@t> salud.unm.edu (Jeffrey Thompson) Date: Wed Jan 13 13:04:41 2010 Subject: [Histonet] H&E on frozen sections Message-ID: <4B4DB6B0.D1F6.004D.1@salud.unm.edu> Hello, This is a question that has probably been asked of the group many times before. I would like to know if anyone can suggest a protocol for H&E stain of paraformaldehyde perfused rat brain sections collected at 10 microns on a cryostat? And, by the way, is there a method to search histonet for topics that have likely been covered before? Thanks, Jeff Thompson University of New Mexico Health Sciences Center Department of Neurology MSC11 6035 Domenici Hall / NRF Room 1311 1101 Yale Blvd. NE Albuquerque, NM 87131 USA From mpence <@t> grhs.net Wed Jan 13 13:36:39 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jan 13 13:36:43 2010 Subject: [Histonet] Slide marking pens Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3CFF@is-e2k3.grhs.net> I am wondering if anyone can tell me where to get slide marking pens that the Pathologist would mark an area of interest on the cover slipped side of a slide. Cytologist use these all the time. hey most often have blue ink. Thanks for any help I can get. Mike From Maria.Katleba <@t> stjoe.org Wed Jan 13 13:45:58 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Jan 13 13:46:08 2010 Subject: [Histonet] RE: Slide marking pens In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3CFF@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3CFF@is-e2k3.grhs.net> Message-ID: HI Mike, Try BBC Biochemical at 1-800-635-4477 Cyt-O-Dot pens item #9001 it's a four pack (one of each colour) green blue red and black Call for current price.... www.BBCUS.com They are great!!!!! Maria Katleba HT (ASCP) MS Queen of the Valley medical Center Pathology Dept. Mgr 707-257-4076 www.thequeen.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide marking pens I am wondering if anyone can tell me where to get slide marking pens that the Pathologist would mark an area of interest on the cover slipped side of a slide. Cytologist use these all the time. hey most often have blue ink. Thanks for any help I can get. Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From dmlongoria <@t> ecrmc.org Wed Jan 13 13:51:19 2010 From: dmlongoria <@t> ecrmc.org (Diana Martinez-Longoria) Date: Wed Jan 13 13:52:22 2010 Subject: [Histonet] Slide marking pens In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3CFF@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3CFF@is-e2k3.grhs.net> Message-ID: Hi, The pathologist use pilot extra fine point permanent marker, cat# SCA-UF. The ink is blue. You can google the information. It has been a while since we have ordered that I don't remember where we got them. Hope this helps. Diana Martinez-Longoria Histology Laboratory/Histology Technician El Centro Regional Medical Center 1415 Ross Ave El Centro CA 92243 (760) 339-7267 (760)482-5365 fax dmlongoria@ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. From: Mike Pence Sent: Wed 1/13/2010 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide marking pens I am wondering if anyone can tell me where to get slide marking pens that the Pathologist would mark an area of interest on the cover slipped side of a slide. Cytologist use these all the time. hey most often have blue ink. Thanks for any help I can get. Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Jan 13 13:53:06 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jan 13 13:53:09 2010 Subject: [Histonet] Slide marking pens In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3CFF@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3CFF@is-e2k3.grhs.net> Message-ID: I order these from Staples, formerly Corporate Express. Pens, xylene free marker, black DZ PIL44102 Pens, xylene free marker, green DZ PIL44103 Pens, xylene free marker, red DZ PIL44104 Pens, xylene free marker, blue DZ PIL44105 Hope this helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 14:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide marking pens I am wondering if anyone can tell me where to get slide marking pens that the Pathologist would mark an area of interest on the cover slipped side of a slide. Cytologist use these all the time. hey most often have blue ink. Thanks for any help I can get. Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From mpence <@t> grhs.net Wed Jan 13 14:01:38 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jan 13 14:01:42 2010 Subject: [Histonet] Slide marking pens In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D00@is-e2k3.grhs.net> Thanks for all the rapid replies. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, January 13, 2010 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens I order these from Staples, formerly Corporate Express. Pens, xylene free marker, black DZ PIL44102 Pens, xylene free marker, green DZ PIL44103 Pens, xylene free marker, red DZ PIL44104 Pens, xylene free marker, blue DZ PIL44105 Hope this helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 14:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide marking pens I am wondering if anyone can tell me where to get slide marking pens that the Pathologist would mark an area of interest on the cover slipped side of a slide. Cytologist use these all the time. hey most often have blue ink. Thanks for any help I can get. Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NLinke <@t> mednet.ucla.edu Wed Jan 13 14:23:54 2010 From: NLinke <@t> mednet.ucla.edu (Linke, Noelle) Date: Wed Jan 13 14:24:02 2010 Subject: [Histonet] job opening at UCLA In-Reply-To: <074888B61A12C549A319A7DF0DBE39DA476280D9@EMGMB1.ad.medctr.ucla.edu> References: <074888B61A12C549A319A7DF0DBE39DA476280D9@EMGMB1.ad.medctr.ucla.edu> Message-ID: <0C96F0BFE078D74C91A1C541D24A6AE49765D9EC@EMGMB1.ad.medctr.ucla.edu> Hi all, I have an opening for a Histotech II or III in the histology lab. For this position, an HTL is required for a III, HT for a II. We are looking for someone with 5+ years of experience in routine histology, manual specials, automated special stain experience is always a help. The hours for this position are 8:00pm-4:30am. To apply, enter the job number H51527 in the 'Find Job Code' box. https://jobs2.mednet.ucla.edu/css_external/CSSPage_BrowseJobs.ASP Salary range for this position is $34.93-$45.18 per hour DOE for Histotech II, and $36.83-47.66 per hour DOE for Histotech III. There will also be additional shift differential. If you are interested, please let me know! Thanks, Noelle No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology & Laboratory Medicine David Geffen School of Medicine at UCLA Phone: 310-825-7397 Pager: 97471 nlinke@mednet.ucla.edu ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From MSHERWOOD <@t> PARTNERS.ORG Wed Jan 13 14:50:17 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jan 13 14:50:22 2010 Subject: [Histonet] Slide marking pens In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3D00@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3D00@is-e2k3.grhs.net> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F28@PHSXMB30.partners.org> I have a question re: these pens. We are a research lab that does histology for our group. We mark our cassettes and slides with special marking pens (currently Statmark Pen), but we are having a problem with the ink wearing off after processing. We have tried marking over the info repeatly, but the ink still comes off! Are these pens for this purpose? Or can you recommend a good marking pen for us? Thanks in advance. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 3:02 PM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens Thanks for all the rapid replies. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, January 13, 2010 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens I order these from Staples, formerly Corporate Express. Pens, xylene free marker, black DZ PIL44102 Pens, xylene free marker, green DZ PIL44103 Pens, xylene free marker, red DZ PIL44104 Pens, xylene free marker, blue DZ PIL44105 Hope this helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 14:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide marking pens I am wondering if anyone can tell me where to get slide marking pens that the Pathologist would mark an area of interest on the cover slipped side of a slide. Cytologist use these all the time. hey most often have blue ink. Thanks for any help I can get. Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From histosearch <@t> gmail.com Wed Jan 13 14:52:37 2010 From: histosearch <@t> gmail.com (HistoLab) Date: Wed Jan 13 14:52:41 2010 Subject: [Histonet] Slide marking pens Message-ID: <521c6d261001131252o792622adkaff35972c18b1251@mail.gmail.com> Gorilla Scientific sells starmark marking pens. They are resistant to aqueous based stains, alcohols, xylene and xylene substitutes. From carrolpb <@t> umdnj.edu Wed Jan 13 14:58:28 2010 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Wed Jan 13 14:58:38 2010 Subject: [Histonet] Slide marking pens In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F28@PHSXMB30.partners.org> References: <661949901A768E4F9CC16D8AF8F2838C017A3D00@is-e2k3.grhs.net> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F28@PHSXMB30.partners.org> Message-ID: <4B4E33F4.3080304@umdnj.edu> > but the ink still comes off! Are these pens for this purpose? > Or can you recommend a good marking pen for us? yep... a pencil ;) From Pam.Bakken <@t> childrensmn.org Wed Jan 13 15:09:43 2010 From: Pam.Bakken <@t> childrensmn.org (Pam Bakken) Date: Wed Jan 13 15:10:00 2010 Subject: [Histonet] New Job Posting - Minneapolis/St. Paul MN Message-ID: <4B4DE237.37F9.0000.0@childrensmn.org> Job Description Job Title: Anatomic Pathology Scientist Job ID: 19854 Location: St. Paul Full/Part Time: Full-Time Regular/Temporary: Regular Work Schedule Std Hrs: 40 hours, 1.0 Shift: Days Shift Length: 8 hours Weekend Coverage: e4th Call Coverage: Department Overview The laboratories at Children's Hospitals and Clinics are full service facilities that offer a complete spectrum of laboratory techniques for the evaluation of pediatric disorders. Laboratories are located on both the Minneapolis and St. Paul campuses and provide services on an inpatient and outpatient basis. Disciplines include Chemistry, Hematology, Microbiology, Virology, Serology, Blood Bank, Immunology/Flow Cytometry, Histology and Pathology. Position Summary Accessions specimens in the pathology computer system and chooses workload along with the appropriate CPT code. Performs gross examination of all tissue submitted to the pathology lab. Prepares non-gyn. cytology for staining. Prepares tissue for microscopic examination by a Pathologist. Assists pathologist with autopsy exams. Acts as a resource for histology services. Collaborates with the health care team to accurately and efficiently provide laboratory services. Communicates results to necessary individuals. Qualifications Graduate from a Histology Technician program with HT or HTL degree. ASCP Certification for HT or HTL or eligible - required to obtain within first twelve months of employment. Physical Demands The following lists only the physical demands that have been identified as frequent or continuous for this position. A more detailed list is available upon request. ?Walking/Standing ?Repetitive Movement - hands/wrists ?Simple Grasping - both hands ?Firm Grasping - Both Hands ?Fine Manipulating - Both Hands ?Keyboarding - both hands EEO Statement Children's Hospitals and Clinics of Minnesota is an equal opportunity employer and is committed to a diverse workforce. Children's also participates in E-Verify. To apply, please click on link below and enter Job ID # 19854 https://eprod89.pshr.childrensmn.org/psp/EXTAPP/EMPLOYEE/HRMS/c/HRS_HRS.HRS_APP_SCHJOB.GBL?FolderPath=PORTAL_ROOT_OBJECT.HC_HRS_APP_SCHJOB_GBL&IsFolder=false&IgnoreParamTempl=FolderPath%2cIsFolder Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From trathborne <@t> somerset-healthcare.com Wed Jan 13 15:11:15 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jan 13 15:11:20 2010 Subject: [Histonet] Slide marking pens In-Reply-To: <521c6d261001131252o792622adkaff35972c18b1251@mail.gmail.com> Message-ID: We use Tissue-Tek Marking Pencils for cassettes and slides. No smearing. We purchase them through Allegiance, catalog #M7321-10. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of HistoLab Sent: Wednesday, January 13, 2010 3:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide marking pens Gorilla Scientific sells starmark marking pens. They are resistant to aqueous based stains, alcohols, xylene and xylene substitutes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From shive003 <@t> umn.edu Wed Jan 13 15:20:33 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Jan 13 15:20:36 2010 Subject: [Histonet] veterinary IHC question - BVD Ab Message-ID: <0743307C8F884BE581BA7B2A8BF83980@auxs.umn.edu> Hi all, I've been using the BVD clone 15C5 for years, but need to find another vendor source with reactivity to another clone. Does anyone have experience with and recommendations for a commercial source of a monoclonal BVD antibody that has strong reactivity? This would be for Type 2 BVD virus. Thanks in advance, Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) From LRaff <@t> uropartners.com Wed Jan 13 15:09:43 2010 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Wed Jan 13 15:22:20 2010 Subject: FW: [Histonet] Re: Bladder Biopsies Message-ID: Bob--I believe we had only minor adjustments to make in our immuno staining when we switched from a VIP processor to a microwave. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, January 13, 2010 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Bladder Biopsies In answer to a query about microwave processing of urinary bladder biopsy specimens Lester Raff MD (Uropartners, Winchester IL) notes: >>We microwave process our bladder biopsies on the same cycle as prostate biopsies.<< Good to know this. Do the immunostains require any adjustment - particularly the racemase stain, notorious for difficulty in adjusting to different fixations? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET NOD32 Antivirus, version of virus signature database 4767 (20100113) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __________ Information from ESET NOD32 Antivirus, version of virus signature database 4768 (20100113) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com From histonet.nospam <@t> vneubert.com Wed Jan 13 15:09:20 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Jan 13 15:23:29 2010 Subject: [Histonet] Slide marking pens In-Reply-To: <4B4E33F4.3080304@umdnj.edu> References: <661949901A768E4F9CC16D8AF8F2838C017A3D00@is-e2k3.grhs.net> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F28@PHSXMB30.partners.org> <4B4E33F4.3080304@umdnj.edu> Message-ID: <4B4E3680.20802@vneubert.com> Should've kept it for Friday's fun :-D Anyway, I guess that there is no ink that will withstand several hours in both watery and organic solvants. I used Securline Lab Markers II, even those will faint slightly when processing. Can be scrubbed of with a HCl-ethanole-water solution. > > but the ink still comes off! Are these pens for this purpose? > > Or can you recommend a good marking pen for us? > > yep... a pencil ;) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Wed Jan 13 15:24:29 2010 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Jan 13 15:24:34 2010 Subject: [Histonet] Slide marking pens In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F28@PHSXMB30.partners.org> Message-ID: I have had very good luck with the Surgipath "Marking Pens" Cat.#01880. I've tried many & they are the only ones that can stand up to my high pH Heat Induced Epitope Retrieval (HIER). Best of Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, January 13, 2010 2:50 PM To: Mike Pence; Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens I have a question re: these pens. We are a research lab that does histology for our group. We mark our cassettes and slides with special marking pens (currently Statmark Pen), but we are having a problem with the ink wearing off after processing. We have tried marking over the info repeatly, but the ink still comes off! Are these pens for this purpose? Or can you recommend a good marking pen for us? Thanks in advance. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 3:02 PM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens Thanks for all the rapid replies. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, January 13, 2010 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens I order these from Staples, formerly Corporate Express. Pens, xylene free marker, black DZ PIL44102 Pens, xylene free marker, green DZ PIL44103 Pens, xylene free marker, red DZ PIL44104 Pens, xylene free marker, blue DZ PIL44105 Hope this helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 14:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide marking pens I am wondering if anyone can tell me where to get slide marking pens that the Pathologist would mark an area of interest on the cover slipped side of a slide. Cytologist use these all the time. hey most often have blue ink. Thanks for any help I can get. Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Jan 13 15:28:17 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jan 13 15:28:20 2010 Subject: [Histonet] Slide marking pens In-Reply-To: References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F28@PHSXMB30.partners.org> Message-ID: Remember that Surgipath is now Leica... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Wednesday, January 13, 2010 16:24 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens I have had very good luck with the Surgipath "Marking Pens" Cat.#01880. I've tried many & they are the only ones that can stand up to my high pH Heat Induced Epitope Retrieval (HIER). Best of Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, January 13, 2010 2:50 PM To: Mike Pence; Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens I have a question re: these pens. We are a research lab that does histology for our group. We mark our cassettes and slides with special marking pens (currently Statmark Pen), but we are having a problem with the ink wearing off after processing. We have tried marking over the info repeatly, but the ink still comes off! Are these pens for this purpose? Or can you recommend a good marking pen for us? Thanks in advance. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 3:02 PM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens Thanks for all the rapid replies. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, January 13, 2010 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens I order these from Staples, formerly Corporate Express. Pens, xylene free marker, black DZ PIL44102 Pens, xylene free marker, green DZ PIL44103 Pens, xylene free marker, red DZ PIL44104 Pens, xylene free marker, blue DZ PIL44105 Hope this helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 14:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide marking pens I am wondering if anyone can tell me where to get slide marking pens that the Pathologist would mark an area of interest on the cover slipped side of a slide. Cytologist use these all the time. hey most often have blue ink. Thanks for any help I can get. Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From sbreeden <@t> nmda.nmsu.edu Wed Jan 13 15:29:12 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jan 13 15:30:24 2010 Subject: [Histonet] Slide marking pens In-Reply-To: <4B4E3680.20802@vneubert.com> References: <661949901A768E4F9CC16D8AF8F2838C017A3D00@is-e2k3.grhs.net> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F28@PHSXMB30.partners.org><4B4E33F4.3080304@umdnj.edu> <4B4E3680.20802@vneubert.com> Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46D9B@nmdamailsvr.nmda.ad.nmsu.edu> I hate pencil and refuse to use them! They smear and blur more than any "specialized" histo slide/cassette markers! I just replied to a separate post saying that as long as your NBF is not "old" (like more than a couple days) and that you let the cassette sit for a minute or two before you drop it into solution to make sure it's ink is completely dry, I've not had any problems. Besides, I think every pen manufactured for our use has an "off day" because I've seen every brand have an occasional glitch. Not that this is a good thing, but it seems to be universal. Has something to do with the phase of the moon and what color socks you're wearing. May the Force be with you... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From tdobersztyn <@t> chmca.org Wed Jan 13 15:34:43 2010 From: tdobersztyn <@t> chmca.org (tdobersztyn@chmca.org) Date: Wed Jan 13 15:34:50 2010 Subject: [Histonet] Fabric Softener sheets Message-ID: Hello all, When using a dryer sheet in the cryostat to reduce static- has anyone experienced an increased amount of artifact with fluorescein stained slides??? We will occasionally get a slide that has lots of fluorescent fibers all over the section! I vaguely remember being told that dryer sheets are not good for labs that cut cryostat sections for either muscle histochemistries /or kidney sections for immunofluorescent staining... I think using a small container of alcohol placed into the cryostat will also help reduce static- These dry months kill us!!!! Thanks in advance Theresa Theresa R Dobersztyn HT (ASCP) Senior Technologist-Histology/Electron Microscopy Labs Dept. of Pathology and Laboratory Medicine Phone: 330-543-8279 Fax: 330-543-3226 tdobersztyn@chmca.org Akron Children's Hospital - Proud winner of the NorthCoast 99 "Best Workplace" award! ************************************************************************* This electronic mail transmission, including any attached files, may contain confidential and/or privileged information for the sole use of the intended recipient(s). It is not intended for transmission to, or receipt by, any unauthorized parties. Any review, use, distribution, dissemination, downloading, copying or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that you have received this transmission in error. If you have received this transmission in error, please delete it, as well as any copies, from your system without copying it, and notify the sender by reply e-mail. From liz <@t> premierlab.com Wed Jan 13 15:40:37 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jan 13 15:40:41 2010 Subject: [Histonet] new CAP guidelines for digital imaging Message-ID: Hello All Someone mentioned about a week ago or so about new CAP guidelines for digital imaging. If anyone has a copy of the questions from CAP and would be willing to share them with me I would greatly appreciate it. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From janderson <@t> halozyme.com Wed Jan 13 15:50:53 2010 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Wed Jan 13 15:51:00 2010 Subject: [Histonet] requirements for certification Message-ID: <5F7CC9B788911848A79BC83453A3D6530360F0F208@Tomlinson.hti.com> Hello. I'm thinking about getting my HTL certification. I've been a bench scientist in both academia and biotech for 21 years, which has included pre-clinical animal research and subsequent histological applications. I've set up an histology lab and have a lot of IHC but only basic staining (mostly H&E), as well as loads of animal tissue processing and some human tissues. I have a liberal arts degree (an AB) with a biology major and a chemistry minor. We have both a DVM and a few MD's on site, but for pre-clinical and clinical research consult. I would appreciate any insight on getting certified through an online program, how many hours is required, and what kind of mentorship is necessary. Thank you for your insights and time! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. From liz <@t> premierlab.com Wed Jan 13 15:56:55 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jan 13 15:57:01 2010 Subject: [Histonet] requirements for certification In-Reply-To: <5F7CC9B788911848A79BC83453A3D6530360F0F208@Tomlinson.hti.com> Message-ID: You might be eligible to sit for the registry already either HT or HTL, you just need to have the correct number of credits in biology and chemistry to meet the requirements. I would call ASCP to see if you have the requirements, it seems to me that you have the experience needed you just need to make sure you have the credits. That's my opinion. All you need is one year on the job training with a BS or BA to sit for the HTL. I think I'm correct on this, but if I'm not we'll know soon enough from the replys. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Wednesday, January 13, 2010 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] requirements for certification Hello. I'm thinking about getting my HTL certification. I've been a bench scientist in both academia and biotech for 21 years, which has included pre-clinical animal research and subsequent histological applications. I've set up an histology lab and have a lot of IHC but only basic staining (mostly H&E), as well as loads of animal tissue processing and some human tissues. I have a liberal arts degree (an AB) with a biology major and a chemistry minor. We have both a DVM and a few MD's on site, but for pre-clinical and clinical research consult. I would appreciate any insight on getting certified through an online program, how many hours is required, and what kind of mentorship is necessary. Thank you for your insights and time! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Jan 13 16:01:57 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jan 13 16:02:01 2010 Subject: [Histonet] Slide marking pens In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F28@PHSXMB30.partners.org> References: <661949901A768E4F9CC16D8AF8F2838C017A3D00@is-e2k3.grhs.net> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F28@PHSXMB30.partners.org> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F2B@PHSXMB30.partners.org> Thanks to all who emailed me in regards to this problem. There were many suggestions of different pens (and pencil). I know pencils work, but I agree that they smear. I have used Secureline Markers as one person suggested, but they seemed to dry up fast. One thing I am not doing is letting the ink dry thoroughly before dropping the cassettes into formalin. I will definitely do that and see how that works. It was suggested that I use fresh formalin. That would be costly; we do change it every 3 weeks (we don't have the volume of cassettes that histology labs run). I will let the list know how I fare. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, January 13, 2010 3:50 PM To: Mike Pence; Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens I have a question re: these pens. We are a research lab that does histology for our group. We mark our cassettes and slides with special marking pens (currently Statmark Pen), but we are having a problem with the ink wearing off after processing. We have tried marking over the info repeatly, but the ink still comes off! Are these pens for this purpose? Or can you recommend a good marking pen for us? Thanks in advance. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 3:02 PM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens Thanks for all the rapid replies. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, January 13, 2010 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens I order these from Staples, formerly Corporate Express. Pens, xylene free marker, black DZ PIL44102 Pens, xylene free marker, green DZ PIL44103 Pens, xylene free marker, red DZ PIL44104 Pens, xylene free marker, blue DZ PIL44105 Hope this helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 14:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide marking pens I am wondering if anyone can tell me where to get slide marking pens that the Pathologist would mark an area of interest on the cover slipped side of a slide. Cytologist use these all the time. hey most often have blue ink. Thanks for any help I can get. Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Jan 13 16:02:02 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jan 13 16:02:07 2010 Subject: [Histonet] Paraffin Disposal In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F0B@PHSXMB30.partners.org> References: <1872B4A455B7974391609AD8034C79FC8BD687@LBEXCH01.hchd.local> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F0B@PHSXMB30.partners.org> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F2C@PHSXMB30.partners.org> To all: I stand corrected! I asked our hazardous waste tech about the paraffin waste and he said they should take it (since it has xylene, or in our case, citrisolv in it). Live and learn. Peggy -----Original Message----- From: Sherwood, Margaret Sent: Monday, January 11, 2010 2:05 PM To: 'Scott, Allison D' Subject: RE: [Histonet] Paraffin Disposal Once it is hardened, I put it in the regular waste (haven't had any questions raised about that practice). Would be interested in other responses. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, January 11, 2010 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Disposal Happy New Year to all. How are you all disposing the paraffin that comes off of the tissue processor? Do you consider it as biohazard waste and disposing it as such or are you putting it into the regular trash disposal? I have been asked why I am disposing it the way I do. I consider it as biohazard waste. Your help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Bonnie.Whitaker <@t> osumc.edu Wed Jan 13 16:08:56 2010 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Wed Jan 13 16:09:07 2010 Subject: [Histonet] requirements for certification In-Reply-To: References: <5F7CC9B788911848A79BC83453A3D6530360F0F208@Tomlinson.hti.com> Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60D66A7E@msxc06.OSUMC.EDU> I agree with Liz. It seems with a bio major and chem minor, that should take care of the coursework. You could do the IU online program, but if you are self-motivated, I would just study on my own, if I were you. If you don't pass the first time, then at least you would know how close you were, and could decide to take the course, or just to study a little more. If you can pass the exam, why waste the time and money unnecessarily? It sounds like you probably have a great background and experience already! Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 Bonnie.Whitaker@osumc.edu phone 614.293.5048 fax 614.293.7273 pager 614.346.5013 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, January 13, 2010 4:57 PM To: Jennifer Anderson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] requirements for certification You might be eligible to sit for the registry already either HT or HTL, you just need to have the correct number of credits in biology and chemistry to meet the requirements. I would call ASCP to see if you have the requirements, it seems to me that you have the experience needed you just need to make sure you have the credits. That's my opinion. All you need is one year on the job training with a BS or BA to sit for the HTL. I think I'm correct on this, but if I'm not we'll know soon enough from the replys. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Wednesday, January 13, 2010 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] requirements for certification Hello. I'm thinking about getting my HTL certification. I've been a bench scientist in both academia and biotech for 21 years, which has included pre-clinical animal research and subsequent histological applications. I've set up an histology lab and have a lot of IHC but only basic staining (mostly H&E), as well as loads of animal tissue processing and some human tissues. I have a liberal arts degree (an AB) with a biology major and a chemistry minor. We have both a DVM and a few MD's on site, but for pre-clinical and clinical research consult. I would appreciate any insight on getting certified through an online program, how many hours is required, and what kind of mentorship is necessary. Thank you for your insights and time! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> medsurgpath.com Wed Jan 13 16:20:48 2010 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Wed Jan 13 16:20:51 2010 Subject: [Histonet] requirements for certification In-Reply-To: References: <5F7CC9B788911848A79BC83453A3D6530360F0F208@Tomlinson.hti.com> Message-ID: <62724.68.178.72.125.1263421248.squirrel@webmail.integra.net> Yes, you are correct. One year OJT plus a BA/BS with required # of biology/chemistry credits for HTL. When I tried to call to see if I qualified they said that they can not tell you over the phone. You have to apply (and pay the $200) to see if you qualify. Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > You might be eligible to sit for the registry already either HT or HTL, > you just need to have the correct number of credits in biology and > chemistry to meet the requirements. I would call ASCP to see if you > have the requirements, it seems to me that you have the experience > needed you just need to make sure you have the credits. That's my > opinion. All you need is one year on the job training with a BS or BA > to sit for the HTL. I think I'm correct on this, but if I'm not we'll > know soon enough from the replys. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, Colorado 80308 > office (303) 682-3949 > fax (303) 682-9060 > www.premierlab.com > > > Ship to Address: > 1567 Skyway Drive, Unit E > Longmont, Colorado 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer > Anderson > Sent: Wednesday, January 13, 2010 2:51 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] requirements for certification > > Hello. > > I'm thinking about getting my HTL certification. I've been a bench > scientist in both academia and biotech for 21 years, which has included > pre-clinical animal research and subsequent histological applications. > I've set up an histology lab and have a lot of IHC but only basic > staining (mostly H&E), as well as loads of animal tissue processing and > some human tissues. I have a liberal arts degree (an AB) with a biology > major and a chemistry minor. We have both a DVM and a few MD's on site, > but for pre-clinical and clinical research consult. I would appreciate > any insight on getting certified through an online program, how many > hours is required, and what kind of mentorship is necessary. > > Thank you for your insights and time! > > Jennifer M. Anderson, Scientist > Halozyme Therapeutics, Inc. > 11388 Sorrento Valley Road > San Diego, CA 92121 > 858-704-8333 > janderson@halozyme.com > > ________________________________ > The information transmitted in this email is confidential and is > intended only for the person(s) or entity to which it is addressed. > Delivery of this message to any person other than the intended > recipient(s) is not intended in any way to waive confidentiality or any > applicable privilege. Any review, retransmission, dissemination or other > use of, or taking of any action in reliance upon, this information by > individuals or entities other than the intended recipient is prohibited > by Halozyme and may be in violation of applicable laws. If you received > this in error, please contact the sender and delete/destroy this email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akbitting <@t> geisinger.edu Wed Jan 13 16:34:32 2010 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Jan 13 16:34:45 2010 Subject: [Histonet] Kaiser Permanente Message-ID: <4B4E0428.2B7F.00C9.0@geisinger.edu> If someone from Kaiser Permanente is watching the Histonet, would you contact me offline about a piece of Histology equipment. My rep. (and I'm not mentioning the company!!) is telling me you have something that I'm interested in buying, and I'd like your feedback. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From mbplab <@t> yahoo.com Wed Jan 13 16:44:09 2010 From: mbplab <@t> yahoo.com (Mary Benoit) Date: Wed Jan 13 16:44:13 2010 Subject: [Histonet] Estrogen and Progesterone Receptors Message-ID: <836353.45892.qm@web43138.mail.sp1.yahoo.com> We currently are using Dako antibodies for Estrogen and Progesterone Receptors then followed with ACIS imaging on all infiltrating breast carcinomas.? We generally put a positive control on the bottom of the same slide for each to save a little time and money.? Results are great?.?Fixation is at least 8 hours (never more than 18 hours)?in NBF on a designated processor. My question is our pathologists are concerned when the Estrogen Receptor is strongly positive and the Progesterone is very weak or negative.? He states that the results should be approximately within 10% of each other.? Our policy is to repeat the IHC if there are no internal "normal" ducts within the sample(note that the normal ducts, when present, do stain well).? Repeated results are the same.? I've made a comparison of diagnosis for tumor types (well diff, mod diff, poorly diff, lobular, etc.) and there is an even distribution of these cases.? Does anybody have input to this situation?? From mbplab <@t> yahoo.com Wed Jan 13 16:47:07 2010 From: mbplab <@t> yahoo.com (Mary Benoit) Date: Wed Jan 13 16:47:10 2010 Subject: [Histonet] Estrogen Receptor and Progesterone Receptors Message-ID: <793819.74990.qm@web43143.mail.sp1.yahoo.com> Sorry, I forgot to sign my name. Mary F Benoit MT(ASCP) The Pathology Laboratory 830 Bayou Pines Drive Lake Charles, La ? From Jenny.Gross <@t> cshs.org Wed Jan 13 17:02:47 2010 From: Jenny.Gross <@t> cshs.org (Gross, Jenny) Date: Wed Jan 13 17:03:45 2010 Subject: [Histonet] TMA Repair Message-ID: My organization has spent a great deal of time and effort constructing a tissue microarray. However, we have just been notified that two out of the three TMA blocks have cracks in the bottom portion and are coming off of the cassettes. I wanted to know what other organizations have done to repair this issue so the blocks can still be used. Please advise. Jenny Gross, MPH Manager Women's Cancer Research Institute Samuel Oschin Comprehensive Cancer Institute Jenny Gross, MPH Manager Women's Cancer Research Institute Samuel Oschin Comprehensive Cancer Institute Cedars-Sinai Health System 8700 Beverly Boulevard, Suite 290W Los Angeles, CA 90048 Office: (310) 423-6241 Cell: (323) 428-2338 IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. From pathmaster <@t> yahoo.com Wed Jan 13 18:08:47 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Wed Jan 13 18:08:51 2010 Subject: [Histonet] HTL certification Message-ID: <307173.79641.qm@web111104.mail.gq1.yahoo.com> >From the ASCP website (just google ASCP and HTL certification) Histotechnologist, HTL(ASCP) Application Fee: $210 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; or Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology laboratory in the U.S., Canada or a CAP/The Joint Commission (JCAHO) accredited laboratory within the last ten years. This year of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology) or an appropriately board certified medical scientist. Clinical Laboratory Experience To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Jeff Silverman ? From tifei <@t> foxmail.com Wed Jan 13 20:11:06 2010 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Wed Jan 13 20:13:40 2010 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIEVsaW1pbmF0aW5nIHRoZSBlZGdlIGVmZmVjdCBpbiBJSEMvSUY=?= References: <886476.93238.qm@web65706.mail.ac4.yahoo.com> Message-ID: <201001141011049169838@foxmail.com> Hi all i believe it is due to the wash + antibody accumulation problem happened between the edge of section and the adhesive slide. Even you have circled the section with IHC pen(DAKO), and flattened all soultions evenly, it improved but did not disappear if you stain the section using "floating section" method, no edge effect now. 2010-01-14 TF ???? Rene J Buesa ????? 2010-01-12 13:30:49 ???? histonet; Karen Cai ??? ??? Re: [Histonet] Eliminating the edge effect in IHC/IF Usually that is the result of incomplete fixation. Check your fixation protocol. Ren? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmoody <@t> ameripath.com Wed Jan 13 22:08:53 2010 From: rmoody <@t> ameripath.com (Moody, Robert) Date: Wed Jan 13 22:10:39 2010 Subject: [Histonet] RE: Histonet Digest, Vol 74, Issue 12 In-Reply-To: References: Message-ID: Hi, All we are having problems with chatter in our biopsies their usually on the edge of tissue is the a problem with the cutting or in the handling of the tissue after it is removed from the patient like the biopsies being left out to dry or not put in formalin what are some of your experience with this. Robert Moody HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, January 13, 2010 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 74, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC (Marian Powers) 2. RE: Eliminating the edge effect in IHC/IF (C.M. van der Loos) 3. RE: RE: Eliminating the edge effect in IHC/IF (Sherwood, Margaret ) 4. RE: Eliminating the edge effect in IHC/IF (Rene J Buesa) 5. RE: RE: Eliminating the edge effect in IHC/IF (Della Speranza, Vinnie) 6. RE: RE: Eliminating the edge effect in IHC/IF (Rene J Buesa) 7. RE: RE: Eliminating the edge effect in IHC/IF (Hermina Borgerink) 8. Fw: Waste paraffin and edge effect (Jeffrey Silverman) 9. Re: Sakura iDent (Anne van Binsbergen) 10. Looking for Florida licensed Histologist (Kaye Ryan) 11. RE: Re: Sakura iDent (Cynthia Pyse) 12. AW: [Histonet] Eliminating the edge effect in IHC/IF (Gudrun Lang) 13. Bladder Biopsies (S R) 14. Re: Bladder Biopsies (Lester Raff MD) 15. Re: Eliminating the edge effect in IHC/IF (histology@medsurgpath.com) 16. Part-time Flex Histologist position (Fallak, Alice) 17. RE: Re: Sakura iDent (Nancy Klemme) 18. Re: RE: [Histonet] Problems with MSB stain (John Kiernan) 19. (Used microtome) - Thanks ... (Massimo) 20. (Used microtome) - Thanks ... (Massimo) 21. AW: [Histonet] Problems with MSB stain (Gudrun Lang) 22. Re: Re: Sakura iDent (Anne van Binsbergen) ---------------------------------------------------------------------- Message: 1 Date: Tue, 12 Jan 2010 14:34:33 -0500 From: Marian Powers Subject: [Histonet] IHC To: histonet@lists.utsouthwestern.edu Message-ID: <5d7de0e61001121134g662d43adr3a7c4cd1b0235854@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi: We are looking to start N-ras and B-raf for IHC, is anyone running these antibodies on paraffin? If so, what clone and vendor are you using? Thanks in advance! -- Marian L. Powers, HT(ASCP) Manager, Technical Operations Doctors Pathology Services 1253 College Park Drive Dover, DE 19904 302-677-0000 ------------------------------ Message: 2 Date: Tue, 12 Jan 2010 20:54:35 +0100 From: "C.M. van der Loos" Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Message-ID: <9637c5fe5d7dffc7.4b4ce18b@amc.uva.nl> Content-Type: text/plain; charset=utf-8 Hi all,We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy. One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved. As a result the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely, including a rim around the section. However, to be honest, I am sure my explanation is certainly not always appropriate. Anyone else????Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren??? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com ------------------------------ Message: 3 Date: Tue, 12 Jan 2010 15:28:55 -0500 From: "Sherwood, Margaret " Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF To: "C.M. van der Loos" , Cc: kcai@prosci-inc.com Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F19@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" Actually, when I read about the problem, my thought was that if you don't circle your tissue with an immunopen or wax pencil, then the reagents (esp. primary antibody)might not cover the tissue evenly as you say. So I tend to agree with you. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Tuesday, January 12, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF Hi all,We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy. One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved. As a result the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely, including a rim around the section. However, to be honest, I am sure my explanation is certainly not always appropriate. Anyone else????Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 4 Date: Tue, 12 Jan 2010 12:40:16 -0800 (PST) From: Rene J Buesa Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, "C.M. van der Loos" Cc: kcai@prosci-inc.com Message-ID: <661401.32335.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 Hi Dr. van der Loos: I had also experienced that artifact caused by less than necessary reagents, but that happens??mostly when IHC??is done manually, or when the autostainer delivers less amount than required or programmed. IF we assume that the colleague with the question did the IHC manually, your explanation can be accepted, otherwise, poor fixation is a valid??cause to the problem. Ren?? J. --- On Tue, 1/12/10, C.M. van der Loos wrote: From: C.M. van der Loos Subject: RE: Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com, rjbuesa@yahoo.com, cforster@umn.edu Date: Tuesday, January 12, 2010, 2:54 PM Hi all, We also have observed this phenomenon many times. But sorry Colleen and Rene,??I don't believe??that an??fixation issue??is the explanation why the edges are sometimes stronger than the rest. To my opinion this is??a bit too easy.??One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved.??As a result??the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely,??including a rim around the section.??However, to??be honest,??I am sure my??explanation is certainly not always appropriate. Anyone else???? Cheers, Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone:?? +31 20 5665631 ?? From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren??? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com ------------------------------ Message: 5 Date: Tue, 12 Jan 2010 15:40:55 -0500 From: "Della Speranza, Vinnie" Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF To: "'C.M. van der Loos'" , "histonet@lists.utsouthwestern.edu" Cc: "kcai@prosci-inc.com" Message-ID: Content-Type: text/plain; charset="utf-8" I think we'll agree that there are different scenarios so that the solution is not a one size fits all. For example, darkened staining around the periphery of needle core biopsies is not uncommon with even tinctorial stains, often thought to be the result of drying of the tissue during the collection of the sample. Years ago I came across an article maintaining that the dark staining on the periphery of needle cores was in fact due to the "trauma" of the needle cutting into the sampled organ. I've since forgotten the author's name and wish I could get my hands on that reference. So I agree with Chris that there doesn't appear to be one simple answer to prevent this artifact and while fixation may contribute in some circumstances it's unlikely to be the remedy for all. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Tuesday, January 12, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF Hi all,We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy. One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved. As a result the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely, including a rim around the section. However, to be honest, I am sure my explanation is certainly not always appropriate. Anyone else????Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren??? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 12 Jan 2010 12:41:24 -0800 (PST) From: Rene J Buesa Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF To: "C.M. van der Loos" , histonet@lists.utsouthwestern.edu, MargaretSherwood Cc: kcai@prosci-inc.com Message-ID: <842909.92777.qm@web65701.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Again, provided that you are doing IHC manually. Ren? J. --- On Tue, 1/12/10, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF To: "C.M. van der Loos" , histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Date: Tuesday, January 12, 2010, 3:28 PM Actually, when I read about the problem, my thought was that if you don't circle your tissue with an immunopen or wax pencil, then the reagents (esp. primary antibody)might not cover the tissue evenly as you say.? So I tend to agree with you. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Tuesday, January 12, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF Hi all,We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy. One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved. As a result the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely, including a rim around the section. However, to be honest, I am sure my explanation is certainly not always appropriate. Anyone else????Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone:? +31 20 5665631 From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 12 Jan 2010 16:03:13 -0500 From: "Hermina Borgerink" Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF To: "Rene J Buesa" , "C.M. van der Loos" , , "MargaretSherwood" Cc: kcai@prosci-inc.com Message-ID: <9AEEF1FB6254224AA355ED285F8491653EE04680@EXCHVS2.medctr.ad.wfubmc.edu> Content-Type: text/plain; charset="iso-8859-1" I do my immuno staining manually using ProbeOn Plus slides which employ the capillary action principle. I always monitor the taking up/whicking off of my solutions for each pair of slides throughout the entire staining process and know that my sections never dry out (I incubate using moist chambers with plenty of fluid). I work at a research/diagnostic medical school facility where our primary focus is on experimental tissues, using standardized and rigid guidelines for fixation: 24 hours at 4 degrees overnight in 4% PF, followed by post fixation in 70% ethanol for a few days. Never see the "edge effect" with these sections. However, I do occasionally see the effect using archival diagnostic samples that were fixed in 10% NBF for a minimum of 48 hours, and probably longer. I have therefore always perceived this effect to be caused by extended, rather than incomplete fixation. Hermina -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 12, 2010 3:41 PM To: C.M. van der Loos; histonet@lists.utsouthwestern.edu; MargaretSherwood Cc: kcai@prosci-inc.com Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF Again, provided that you are doing IHC manually. Ren? J. --- On Tue, 1/12/10, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF To: "C.M. van der Loos" , histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Date: Tuesday, January 12, 2010, 3:28 PM Actually, when I read about the problem, my thought was that if you don't circle your tissue with an immunopen or wax pencil, then the reagents (esp. primary antibody)might not cover the tissue evenly as you say.? So I tend to agree with you. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Tuesday, January 12, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF Hi all,We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy. One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved. As a result the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely, including a rim around the section. However, to be honest, I am sure my explanation is certainly not always appropriate. Anyone else????Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone:? +31 20 5665631 From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren? J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 12 Jan 2010 14:30:46 -0800 (PST) From: Jeffrey Silverman Subject: [Histonet] Fw: Waste paraffin and edge effect To: histonet@lists.utsouthwestern.edu Message-ID: <13966.65872.qm@web111106.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 --- Hello everyone, ? Our?system's ?hazardous chemical waste consultants changed our method of disposal- we had been solidifying the paraffin in a hood and then red bagging it, ie treating it as regulated medical (biohazard) waste. ? But, due to it's contamination with xylene, the very reason for our disposing of it, the paraffin is really hazardous chemical waste. Believe it or not, EPA regs prohibit most hospitals from treating their chemical waste and the consultants worried that solidifying it under the hood was considered "treating" the waste.? Yeah right!!? ?Anyway, the blocks of solidified paraffin are now dumped in?it's own specially labelled drum and has become a part of our hazardous waste stream, manifested like the xylene and dye waste. I'd be careful about putting hazardous chemicals into?regulated medical waste. ? Interestingly, our system recently inquired if formalin in discarded surgicals needs to be decanted before disposal- many hospitals are doing just that, but most of those, but not all, recycle formalin (yuk!).?Our disposal facility informed us that as long as the containers are plastic, ie are flammamble, they can be incinerated with the formalin and there is no need to decant. ?Glory be!! ? In IHC slides, edge effect, or more intense, sometimes?nonspecific, staining at the periphery of the tissue, can be caused? by more intense fixation of a block at the periphery,?drying out of the edges of a block before fiation, ?and/or by some degree of drying of antibodies and detection chemistry reagents during?staining- this cause is more common in manually stained slides rather than those stained on automated stainers. ? ? Also electrocautery?used during the excision can also cause intense staining at the edges of specimens. ? Jeffrey S. Silverman HT HTL QIHC (ASCP) Southside Hospital- NSLIJ Health System Pathologists' Assistant and Laboratory Safety Officer ------------------------------ Message: 9 Date: Wed, 13 Jan 2010 13:15:36 +0400 From: Anne van Binsbergen Subject: [Histonet] Re: Sakura iDent To: Denise Van Eaton Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Denise sadly no resolution also sadly very little response from the histonet - except for Rene - he and i did some troubleshooting to try to see if i had missed anything - we had the same thoughts so ... i am still sitting with the iDent in its box, unused, until someone, somewhere can convince me that the print does not wipe off with xylene - which will be hard to do seeing that i wiped it off myself and saw with my own eyes that xylene removes the ink!! Sakura - if you are reading this - i am not a happy camper Annie 2010/1/12 Denise Van Eaton > Hi Anne, > > We have been checking into cassette printers. Obviously, if we can bring > one in for a demonstration we will but I thought the iDent looked like a > good place to start. I noticed your problem (on the Histonet) with the ink > rubbing off after the xylene. I never saw any responses from the rest of the > Histonetters... did you resolve the problem? Assuming you (or Sakura) did, > what was the trick? > > Thank you in advance for sharing your "fix" for a potential problem. > > Denise Van Eaton HT(ASCP) > Litton Pathology Associates > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 10 Date: Wed, 13 Jan 2010 08:58:54 -0500 From: "Kaye Ryan" Subject: [Histonet] Looking for Florida licensed Histologist To: Message-ID: Content-Type: text/plain; charset="us-ascii" North Florida Dermatology Associates located in Jacksonville, Florida is looking for a full time histologist. Must be eligible for Florida State Technologist license. The lab is a small private lab with good benefits and minimal stress. Responsibilities include routine histology and some IHC techniques. If interested, please contact me or our Personnel Director at 904-398-0547, ext. 1106. Kaye Ryan North Florida Dermatology Associates Histology Lab Supervisor (904) 398-0547 Ext. 2004 ------------------------------ Message: 11 Date: Wed, 13 Jan 2010 09:03:34 -0500 From: "Cynthia Pyse" Subject: RE: [Histonet] Re: Sakura iDent To: "'Anne van Binsbergen'" , "'Denise Van Eaton'" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <000001ca9459$32066300$96132900$@com> Content-Type: text/plain; charset="us-ascii" Anne You might want to try going up the Sakura chain of command. I had trouble with a coverslipper, after contacting the regional manager it was amazing the service it received. Good luck. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Wednesday, January 13, 2010 4:16 AM To: Denise Van Eaton Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Sakura iDent Hi Denise sadly no resolution also sadly very little response from the histonet - except for Rene - he and i did some troubleshooting to try to see if i had missed anything - we had the same thoughts so ... i am still sitting with the iDent in its box, unused, until someone, somewhere can convince me that the print does not wipe off with xylene - which will be hard to do seeing that i wiped it off myself and saw with my own eyes that xylene removes the ink!! Sakura - if you are reading this - i am not a happy camper Annie 2010/1/12 Denise Van Eaton > Hi Anne, > > We have been checking into cassette printers. Obviously, if we can bring > one in for a demonstration we will but I thought the iDent looked like a > good place to start. I noticed your problem (on the Histonet) with the ink > rubbing off after the xylene. I never saw any responses from the rest of the > Histonetters... did you resolve the problem? Assuming you (or Sakura) did, > what was the trick? > > Thank you in advance for sharing your "fix" for a potential problem. > > Denise Van Eaton HT(ASCP) > Litton Pathology Associates > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 13 Jan 2010 15:32:19 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Eliminating the edge effect in IHC/IF To: "'Karen Cai'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <21FEAA12A3C44D2FBE8EC603949E9CB3@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" My favourite for intense specific staining at the edges vs. faint specific staining in the center is poor fixation. I think drying artefacts or crush artefacts would lead rather to unspecific background staining. Gudrun Lang Histolab, Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Karen Cai Gesendet: Montag, 11. J?nner 2010 20:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Eliminating the edge effect in IHC/IF Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 13 Jan 2010 09:44:03 -0500 From: S R Subject: [Histonet] Bladder Biopsies To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello Everyone! I have a couple of questions. I work for a urology group. As of right now i only work with prostate bx's. They want to add Bladder bx's into the mix. As of right now i microwave process them. I was wondering if anyone eles does this and if you use a different procedure than you would use for processing the prostate biopsies, and would anyone be willing to share? Thanks in Advanced Sammy _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/196390709/direct/01/ ------------------------------ Message: 14 Date: Wed, 13 Jan 2010 09:09:54 -0600 From: "Lester Raff MD" Subject: [Histonet] Re: Bladder Biopsies To: Message-ID: Content-Type: text/plain; charset="us-ascii" We microwave process our bladder biopsies on the same cycle as prostate biopsies. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 ------------------------------ Message: 15 Date: Wed, 13 Jan 2010 07:50:50 -0800 (PST) From: histology@medsurgpath.com Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: "Karen Cai" Cc: histonet@lists.utsouthwestern.edu Message-ID: <61410.68.178.72.125.1263397850.squirrel@webmail.integra.net> Content-Type: text/plain;charset=iso-8859-1 Hi Karen/Histonet, This is a problem we have been working on for some time with our IHC. We have ruled out fixation as the source of the problem, and we are in Oregon so we don't believe we are having a drying out issue. I have also tried different detection kits with both having this artifact. Recently we were wondering if it could be a problem with our blower. Is it possible that it is blowing too hard, or that the rinses are too hard? We have tried hand staining twice, once with perfect results, once with the same artifact. Any additional advice you can give will be greatly appreciated. Thank you, once again, Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (formerly Cutting Edge Histology) (503)443-2157 > Hi, > I have a question for the generous input. When I do the IHC or IF, it > seems very common that the intensity of the edge area of the tissue is > always stronger than the central tissue part. Is it possible to > eliminate this and make the staining evenly distributed around the whole > tissue section? > > Your kind help is greatly appreciated, > > > Thanks in advance, > > Best, > Karen > > Karen Cai > Research Scientist > Prosci Incorporated > (858) 513-2638 x 204 > (858) 513-2692 Fax > www.prosci-inc.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 16 Date: Wed, 13 Jan 2010 10:14:18 -0600 From: "Fallak, Alice" Subject: [Histonet] Part-time Flex Histologist position To: Message-ID: Content-Type: text/plain; charset="us-ascii" We have a part-time/Flex Histologist position open at United Hospital and Medical Center in Kenosha,Wi. Anyone interested can contact our Human Resource Dept. at (262)656-2116. Alice Fallak Histology coordinator United Hosp. & Med. Center ------------------------------ Message: 17 Date: Wed, 13 Jan 2010 08:21:12 -0800 From: Nancy Klemme Subject: RE: [Histonet] Re: Sakura iDent To: Anne van Binsbergen , Denise Van Eaton Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <782E3A02C2EB2347BEA6DEA69DC7AB8669D9A4399E@sfamail.SAKURAUS.LOCAL> Content-Type: text/plain; charset="us-ascii" Dear Annie, I am writing you from California where our Wednesday workday is beginning and you are preparing for your Wednesday to draw to a close. Although the majority of Sakura Finetek products are carried by all Sakura Finetek entities, the iDent is not one that is available from Sakura Finetek USA in North America. If most Histonet subscribers are from the U.S., that may explain the "very little response from the histonet" that you noted. I am sorry about your cassette labeler issues and have forwarded your posting to the gentleman who is in charge of Sakura Finetek Middle East. His name is Mr. Ghaleb Alkhaseb and I am sure he will be in contact with you very soon after reading it. I do not know what Sakura contact information you have been using, but I know that Mr. Alkhaseb will make sure that you will have the most current information for a more direct company contact. I do expect your iDent situation will be corrected and resolved to your satisfaction very soon. >From 12 hours behind you, I offer my kindest regards. Nancy Klemme, HT(ASCP) Edu. Svcs. Dir. - Sakura Finetek USA, Inc. - Torrance, CA 800-725-8723 x7879 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Wednesday, January 13, 2010 1:16 AM To: Denise Van Eaton Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Sakura iDent Hi Denise sadly no resolution also sadly very little response from the histonet - except for Rene - he and i did some troubleshooting to try to see if i had missed anything - we had the same thoughts so ... i am still sitting with the iDent in its box, unused, until someone, somewhere can convince me that the print does not wipe off with xylene - which will be hard to do seeing that i wiped it off myself and saw with my own eyes that xylene removes the ink!! Sakura - if you are reading this - i am not a happy camper Annie 2010/1/12 Denise Van Eaton > Hi Anne, > > We have been checking into cassette printers. Obviously, if we can bring > one in for a demonstration we will but I thought the iDent looked like a > good place to start. I noticed your problem (on the Histonet) with the ink > rubbing off after the xylene. I never saw any responses from the rest of the > Histonetters... did you resolve the problem? Assuming you (or Sakura) did, > what was the trick? > > Thank you in advance for sharing your "fix" for a potential problem. > > Denise Van Eaton HT(ASCP) > Litton Pathology Associates > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 13 Jan 2010 11:24:19 -0500 From: John Kiernan Subject: Re: RE: [Histonet] Problems with MSB stain To: Stephen.Eyres@sanofi-aventis.com Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; CHARSET=US-ASCII The unduly blue sections are all from Animals 12, 13 and 14. Anything different about these specimens? The blue and red sections surely must look different under the microscope. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Stephen.Eyres@sanofi-aventis.com Date: Wednesday, January 13, 2010 10:12 Subject: RE: [Histonet] Problems with MSB stain To: jkiernan@uwo.ca > Hi John, > > Many thanks for the rapid response. The slides look fine down the microscope except for a few that macroscopically look blue. However because the MSBs will be sent away for evaluation, we want to send a consistent set of slides. I attach a picture to show the problem. I can also scan a slide if you prefer, but the image size is large. The paper was very helpful. > > Cheers > > Steve > > From: John Kiernan [mailto:jkiernan@uwo.ca] > Sent: Wednesday, January 13, 2010 6:23 AM > To: Eyres, Stephen R&D/GB > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Problems with MSB stain > > What do the slides look like under the microscope? In a normal liver there shouldn't be much collagen (blue) and there should be plenty of cytoplasm (red). If the preceding nuclear stain was too strong, that could put unwanted bluish shades in the cytoplasm. > > Trichrome methods aren't at their best after formaldehyde fixation; the colours can be improved by soaking the hydrated sections in saturated aqueous picric acid for 30 min or so at 55-60C before staining. Bouin's solution is also used for this purpose, and it is said that the same effect can be achieved with citrate buffer pH4 or with 1% iodine in 2% aqueous KI. See Yu Y, Chapman CM (2003) Masson trichrome stain: postfixation substitutes. J. Histotechnol. 26: 131-134. I've not yet tried these alternatives to picric acid; has anyone else? > > Lendrum's 1962 paper, which includes colour photos, includes many technical tips. More than 40 years ago a med lab technician in Birmingham, UK, suggested this method to me as a way to impart different colours to gliosis (red) and collagenous scarring (blue) in sections of the brains of frogs and other animals in which I'd made surgical lesions dorsal to the optic chiasma. The MSB method showed these changes very well. I recall that we had to buy about 500 grams of brilliant crystal scarlet 6R because it was then cheaper as an an article of commerce than as a bioogical stain. > > I have appended a PDF of Lendrum et al 1962. This won't gwt through to all Histonetters, but it might make it to Stephen.Eyres@sanofi-aventis.com. > > > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: Stephen.Eyres@sanofi-aventis.com > Date: Tuesday, January 12, 2010 11:06 > Subject: [Histonet] Problems with MSB stain > To: histonet@lists.utsouthwestern.edu > > > Hi, > > > > I have just encountered a problem I have never seen before and would > > welcome opinions as to the cause. I work in pharma R&D and had a > > requestto perform MSBs on some liver slides. The results varied > > within the run, > > with a distinct difference in the overall colour of the > > slides; some > > appearing blue and others red. The person performing the > > staining is > > experienced, and our neqas scores are good. When we investigated the > > problem the only difference we could identify was that the bluer > > slideswere stained after 40 minutes drying after being > > cut, whereas the > > redder slides were cut the day before. This suggests that maybe slides > > were incompletely dried and that this affected the staining. Comments > > please. > > > > Cheers > > > > Steve > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 13 Jan 2010 08:50:18 -0800 (PST) From: Massimo Subject: [Histonet] (Used microtome) - Thanks ... To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <939066.56789.qm@web23307.mail.ird.yahoo.com> Content-Type: text/plain; charset=utf-8 ... to all those who kindly responded at my email. Best Regards, Massimo Tosi ================================================= In ricordo di "Nice": "E Argo, che aveva visto Odisseo dopo vent???anni, fu preso dal Fato della nera morte." Omero, Odissea, XVII, 290-327 ------------------------------ Message: 20 Date: Wed, 13 Jan 2010 08:50:18 -0800 (PST) From: Massimo Subject: [Histonet] (Used microtome) - Thanks ... To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <939066.56789.qm@web23307.mail.ird.yahoo.com> Content-Type: text/plain; charset=utf-8 ... to all those who kindly responded at my email. Best Regards, Massimo Tosi ================================================= In ricordo di "Nice": "E Argo, che aveva visto Odisseo dopo vent???anni, fu preso dal Fato della nera morte." Omero, Odissea, XVII, 290-327 ------------------------------ Message: 21 Date: Wed, 13 Jan 2010 17:58:18 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Problems with MSB stain To: Cc: histonet@lists.utsouthwestern.edu Message-ID: <76730F9EC40D4109BDEB62A3B9762C60@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Hi Steve, I have noticed that the CAB-stain, which is also a trichrome-stain (like Trichrome Gomori), shows a blue overall appearence after NBF-fixation alone. After re-fixation in Bouin the staining shows normal red staining of liverslides. Re-fixation is like "antigen-retrieval" and opens the right binding sites for the dyes. Did you re-fix your slides in Bouin? Is there a difference in fixation of the specimen? Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Stephen.Eyres@sanofi-aventis.com Gesendet: Dienstag, 12. J?nner 2010 17:05 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Problems with MSB stain Hi, I have just encountered a problem I have never seen before and would welcome opinions as to the cause. I work in pharma R&D and had a request to perform MSBs on some liver slides. The results varied within the run, with a distinct difference in the overall colour of the slides; some appearing blue and others red. The person performing the staining is experienced, and our neqas scores are good. When we investigated the problem the only difference we could identify was that the bluer slides were stained after 40 minutes drying after being cut, whereas the redder slides were cut the day before. This suggests that maybe slides were incompletely dried and that this affected the staining. Comments please. Cheers Steve _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Wed, 13 Jan 2010 21:25:28 +0400 From: Anne van Binsbergen Subject: Re: [Histonet] Re: Sakura iDent To: Nancy Klemme Cc: "histonet@lists.utsouthwestern.edu" , Denise Van Eaton Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dear Nancy thank you for your email - yes, I know Ghaleb well and have had a working relationship with him for many years here in the middle east. He is indeed trying to extract some sort of resolution from the Sakura European Head office in Zoeterwoude - Ralph, Wim, Chris and Kees-Jan ....my local vendor here in the UAE is excellent and he has witnessed my print rubbing off the cassette face when gently brushed with xylene on gloved finger or a cotton swab. I must just add that it is very strange for me to be battling with a company I have avidly supported and promoted for so many years. I hope that we will resolve this issue and that i will add yet another Sakura workhorse to my already large Sakura stable. kind regards Annie 2010/1/13 Nancy Klemme > Dear Annie, > > I am writing you from California where our Wednesday workday is beginning > and you are preparing for your Wednesday to draw to a close. > > Although the majority of Sakura Finetek products are carried by all Sakura > Finetek entities, the iDent is not one that is available from Sakura Finetek > USA in North America. If most Histonet subscribers are from the U.S., that > may explain the "very little response from the histonet" that you noted. > > I am sorry about your cassette labeler issues and have forwarded your > posting to the gentleman who is in charge of Sakura Finetek Middle East. > His name is Mr. Ghaleb Alkhaseb and I am sure he will be in contact with > you very soon after reading it. I do not know what Sakura contact > information you have been using, but I know that Mr. Alkhaseb will make sure > that you will have the most current information for a more direct company > contact. I do expect your iDent situation will be corrected and resolved to > your satisfaction very soon. > > From 12 hours behind you, I offer my kindest regards. > > Nancy Klemme, HT(ASCP) > Edu. Svcs. Dir. - Sakura Finetek USA, Inc. - Torrance, CA > 800-725-8723 x7879 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van > Binsbergen > Sent: Wednesday, January 13, 2010 1:16 AM > To: Denise Van Eaton > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Sakura iDent > > Hi Denise > sadly no resolution > also sadly very little response from the histonet - except for Rene - he > and > i did some troubleshooting to try to see if i had missed anything - we had > the same thoughts > so ... i am still sitting with the iDent in its box, unused, until someone, > somewhere can convince me that the print does not wipe off with xylene - > which will be hard to do seeing that i wiped it off myself and saw with my > own eyes that xylene removes the ink!! > > Sakura - if you are reading this - i am not a happy camper > > Annie > 2010/1/12 Denise Van Eaton > > > Hi Anne, > > > > We have been checking into cassette printers. Obviously, if we can bring > > one in for a demonstration we will but I thought the iDent looked like a > > good place to start. I noticed your problem (on the Histonet) with the > ink > > rubbing off after the xylene. I never saw any responses from the rest of > the > > Histonetters... did you resolve the problem? Assuming you (or Sakura) > did, > > what was the trick? > > > > Thank you in advance for sharing your "fix" for a potential problem. > > > > Denise Van Eaton HT(ASCP) > > Litton Pathology Associates > > > > > > -- > Anne van Binsbergen (Hope) > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 74, Issue 12 **************************************** From pathmaster <@t> yahoo.com Thu Jan 14 01:14:59 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Thu Jan 14 01:15:05 2010 Subject: [Histonet] IHC edge effect Message-ID: <675027.4320.qm@web111107.mail.gq1.yahoo.com> ? Hi Katelin, ? It would be helpful to view some images- can you post them on Histonet? Just to be sure: You say you have intense peripheral non-specific staining and do not see the expected immunoreactivity in the center of the tissues, or do you see expected reactivity centrally but of weaker intensity? Don't know what more to tell you but some questions come to mind... .. Formalin fixed tissues?? Any microwave? processing or fixation going on? Antigen retrieval? SMA, in my experience, does not require antigen?retrieval. ? Are you staining manually or on an instrument?? If instrument, which one? Is it calibrated and PM'ed to deliver the proper amount of reagent? The progressive involvement and the problem's new appearance in other antibodies suggests maybe a deteriorating problem with an instrument's function. ? ?If manual, do you drag reagent well past the edges of the tssue to more than cover each section?? Is only one person doing the stains? ie?could it be a problem with someone's technique? ? How do you dry your sections? Immediately after cutting place in a 60 degree?C oven. Be sure it is? no more than that, hotter than 60 deg C?will damage sections and immunoreactivity.?Or do you ?air dry first and then oven?? This might allow lifting of the periphery with subsequent entrapment of reagent under the edges that might not be removed with interstep washing. ?Just some more ideas. ? Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant Southside Hospital - NSLIJ Health System --- On Wed, 1/13/10, Katelin Lester wrote: From: Katelin Lester Subject: Re: [Histonet] Fw: Waste paraffin and edge effect To: "Jeffrey Silverman" Date: Wednesday, January 13, 2010, 12:33 PM Hi Jeffrey, I'm curious about what you said about the edges of the tissue having more fixation than the rest of the tissue. I'm curious because that would make sense with some of the uterus sections we are using for our control for SMA, but I'm not sure how that would work for the patient tissue as well. Every SMA I run, control and patient, any area of the slide shows this dark edge staining with minimal staining in the center, as well as nonspecific. We are also seeing it in CK AE1/AE3 and our CD117 has started demonstrating it on previously perfect controls.? Any other ideas or suggestions? Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > > > --- > > > > > > > > Hello everyone, > ? > Our?system's ?hazardous chemical waste consultants changed our method of > disposal- we had been solidifying the paraffin in a hood and then red > bagging it, ie treating it as regulated medical (biohazard) waste. > ? > But, due to it's contamination with xylene, the very reason for our > disposing of it, the paraffin is really hazardous chemical waste. Believe > it or not, EPA regs prohibit most hospitals from treating their chemical > waste and the consultants worried that solidifying it under the hood was > considered "treating" the waste.? Yeah right!!? ?Anyway, the blocks of > solidified paraffin are now dumped in?it's own specially labelled drum and > has become a part of our hazardous waste stream, manifested like the > xylene and dye waste. I'd be careful about putting hazardous chemicals > into?regulated medical waste. > ? > Interestingly, our system recently inquired if formalin in discarded > surgicals needs to be decanted before disposal- many hospitals are doing > just that, but most of those, but not all, recycle formalin (yuk!).?Our > disposal facility informed us that as long as the containers are plastic, > ie are flammamble, they can be incinerated with the formalin and there is > no need to decant. ?Glory be!! > ? > In IHC slides, edge effect, or more intense, sometimes?nonspecific, > staining at the periphery of the tissue, can be caused? by more intense > fixation of a block at the periphery,?drying out of the edges of a block > before fiation, ?and/or by some degree of drying of antibodies and > detection chemistry reagents during?staining- this cause is more common in > manually stained slides rather than those stained on automated stainers. ? > ? > Also electrocautery?used during the excision can also cause intense > staining at the edges of specimens. > ? > Jeffrey S. Silverman HT HTL QIHC (ASCP) > Southside Hospital- NSLIJ Health System > Pathologists' Assistant and Laboratory Safety Officer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histonet.nospam <@t> vneubert.com Thu Jan 14 01:29:22 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Thu Jan 14 01:29:33 2010 Subject: [Histonet] Slide marking pens In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F2B@PHSXMB30.partners.org> References: <661949901A768E4F9CC16D8AF8F2838C017A3D00@is-e2k3.grhs.net> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F28@PHSXMB30.partners.org> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F2B@PHSXMB30.partners.org> Message-ID: <4B4EC7D2.1000706@vneubert.com> Keep the cap on the pen and always store the pen on its tip (even in the lab coats pocket). Won't dry out, for sure. > I have used Secureline Markers as one person suggested, but they seemed to dry > up fast. > > Peggy > From Kjones <@t> upei.ca Thu Jan 14 06:45:42 2010 From: Kjones <@t> upei.ca (Kathleen Jones) Date: Thu Jan 14 06:45:51 2010 Subject: [Histonet] veterinary IHC question - BVD Ab Message-ID: <4B4ED9B70200008B0001D141@grpwise.novell.upei.ca> Hello Jan I have been trying to find a reliable source for a decent BVD antibody for a long time now with no success. Idexx will supply a good antibody for certain research projects, but not for any diagnostic work. Please keep us informed if you can find a supplier. Good luck! Kathy Kathleen Jones Research Technician Pathology/Microbiology AVC - UPEI (902)566-0595 From HornHV <@t> archildrens.org Thu Jan 14 08:02:18 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jan 14 08:02:23 2010 Subject: [Histonet] new CAP guidelines for digital imaging In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8364F@EMAIL.archildrens.org> I am interested in the information as well. Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, January 13, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new CAP guidelines for digital imaging Hello All Someone mentioned about a week ago or so about new CAP guidelines for digital imaging. If anyone has a copy of the questions from CAP and would be willing to share them with me I would greatly appreciate it. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From kc <@t> ka-recruiting.com Thu Jan 14 08:04:16 2010 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Thu Jan 14 08:04:32 2010 Subject: [Histonet] Histology Job Opportunities Message-ID: <1243318705.1263477856199.JavaMail.cfservice@webserver54> Dear Histonet Subscribers, I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. If you're looking for a new job please then please email a resume to kc@ka-recruiting and let me know when you're available to talk. Below is a list of some of the other Histology opportunities we are currently working on. Histotechs/Cytotechs: Connecticut- Histology Operations Manager Southern CA - Histology Supervisor New York City - Surgical Pathology and Histology Supervisor New York City - Histotech 3rd shift Las Vegas, NV - Histotech 3rd shift Central Georgia - Histotech 1st shift Oklahoma - Histotech 1st shift (with opportunity to be promoted to supervisor) Long Island, NY - Cytotech New York City - Cytotech Pennsylvania - Cytology Supervisor Connecticut ? Cytotech If you're interested in learning more about any of these opportunities then please email me a resume and let me know how best to get in touch with you. If none of these are a fit please let me know what you'd be interested in and where you're looking so I can tailor a search for you. With the New Year upon us many of our clients have fresh hiring budgets and will be looking to add people over the next several months. We work on positions at all levels and cover the entire US. To view some additional opportunities please visit our website at www.ka-recruiting.com . Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From tbraud <@t> holyredeemer.com Thu Jan 14 08:22:07 2010 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jan 14 08:22:16 2010 Subject: [Histonet] new CAP guidelines for digital imaging In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8364F@EMAIL.archildrens.org> Message-ID: Me, three Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax -----Original Message----- Hazel V Sent: Thursday, January 14, 2010 09:02 To: Liz Chlipala; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new CAP guidelines for digital imaging I am interested in the information as well. Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, January 13, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new CAP guidelines for digital imaging Hello All Someone mentioned about a week ago or so about new CAP guidelines for digital imaging. If anyone has a copy of the questions from CAP and would be willing to share them with me I would greatly appreciate it. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From trathborne <@t> somerset-healthcare.com Thu Jan 14 08:48:33 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Jan 14 08:48:40 2010 Subject: [Histonet] new CAP guidelines for digital imaging In-Reply-To: Message-ID: Might just as well reply to all. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Terri Braud Sent: Thursday, January 14, 2010 9:22 AM To: Horn, Hazel V; Liz Chlipala; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new CAP guidelines for digital imaging Me, three Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax -----Original Message----- Hazel V Sent: Thursday, January 14, 2010 09:02 To: Liz Chlipala; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new CAP guidelines for digital imaging I am interested in the information as well. Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, January 13, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new CAP guidelines for digital imaging Hello All Someone mentioned about a week ago or so about new CAP guidelines for digital imaging. If anyone has a copy of the questions from CAP and would be willing to share them with me I would greatly appreciate it. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. 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From wlecorch <@t> rwjuhh.edu Thu Jan 14 09:21:14 2010 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Thu Jan 14 09:21:25 2010 Subject: [Histonet] RE: requirements for certification Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71A4AB78AF1@HAMEXMBA.rwjham.local> Histotechnician, HT(ASCP) Application Fee: $185 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Successful completion of a NAACLS accredited Histotechnician program within the last 5 years prior to the date of application for examination; or Route 2: At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, or Associate degree from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO) accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. Clinical Laboratory Experience To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation, Microtomy, Processing, Staining Histotechnologist, HTL(ASCP) Application Fee: $210 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; or Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology laboratory in the U.S., Canada or a CAP/The Joint Commission (JCAHO) accredited laboratory within the last ten years. This year of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology) or an appropriately board certified medical scientist. Clinical Laboratory Experience To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation, Microtomy, Processing, Staining From dimitri.scholz <@t> ucd.ie Thu Jan 14 09:52:04 2010 From: dimitri.scholz <@t> ucd.ie (Dimitri Scholz) Date: Thu Jan 14 09:52:14 2010 Subject: [Histonet] Histology Job Opportunities In-Reply-To: <1243318705.1263477856199.JavaMail.cfservice@webserver54> References: <1243318705.1263477856199.JavaMail.cfservice@webserver54> Message-ID: <004e01ca9531$8494f090$8dbed1b0$%scholz@ucd.ie> Hi KC Carpenter, I am not looking for a Histology Supervisor position (at least not for now), but could be helpful for you and your clients as a consultant. We collected a lot of expertise here in Dublin and offer a Master Course in Imaging starting from September 2010. Earlier this spring, we will offer some stand-alone teaching modules in advanced microscopy methods for PhD students and technicians. Kind regards, Dimitri Dimitri Scholz, PhD, Dr. Sci. Director of Biological Imaging Associate Professor Conway Institute University College Dublin (UCD) Belfield, Dublin 4 Ireland Tel: +353-1 716 6736 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of K.C. Carpenter Sent: 14 January 2010 14:04 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Job Opportunities Dear Histonet Subscribers, I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. If you're looking for a new job please then please email a resume to kc@ka-recruiting and let me know when you're available to talk. Below is a list of some of the other Histology opportunities we are currently working on. Histotechs/Cytotechs: Connecticut- Histology Operations Manager Southern CA - Histology Supervisor New York City - Surgical Pathology and Histology Supervisor New York City - Histotech 3rd shift Las Vegas, NV - Histotech 3rd shift Central Georgia - Histotech 1st shift Oklahoma - Histotech 1st shift (with opportunity to be promoted to supervisor) Long Island, NY - Cytotech New York City - Cytotech Pennsylvania - Cytology Supervisor Connecticut Cytotech If you're interested in learning more about any of these opportunities then please email me a resume and let me know how best to get in touch with you. If none of these are a fit please let me know what you'd be interested in and where you're looking so I can tailor a search for you. With the New Year upon us many of our clients have fresh hiring budgets and will be looking to add people over the next several months. We work on positions at all levels and cover the entire US. To view some additional opportunities please visit our website at www.ka-recruiting.com . Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From KMB01 <@t> grh.org Thu Jan 14 11:29:34 2010 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Thu Jan 14 11:29:38 2010 Subject: [Histonet] Leica CM 1850 UV cryostat Message-ID: Good Morning Histonetters, We have a Leica CM 1850 UV cryostat and I was wondering how often others are using the UV to decontaminate the cryostat. When we bought this I was told to use it only when we suspected TB or the like. BUT we are to treat ALL human tissue as if we knew for certain that it was infectious right? So with this in mind are others using the UV light after every frozen section. We are a small lab doing maybe 10 FS a month. I need to write a procedure on how often this should be done so wanted your option. We do disinfect the cryostat with 70% Alc. Have a great day, Kathy Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From laurie.colbert <@t> huntingtonhospital.com Thu Jan 14 11:40:53 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Jan 14 11:40:57 2010 Subject: [Histonet] Jennifer Hofecker Message-ID: <57BE698966D5C54EAE8612E8941D768307AE3F9A@EXCHANGE3.huntingtonhospital.com> Hi, Does someone have Jennifer's phone number? I have a possible CJD case. Laurie Colbert Huntington Hospital Pasadena ca (626) 397-8620 From arvidsonkristen <@t> yahoo.com Thu Jan 14 12:28:04 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Thu Jan 14 12:28:09 2010 Subject: [Histonet] Automated special stainers Message-ID: <85324.71785.qm@web65710.mail.ac4.yahoo.com> Which automated Special Stainer does everyone like best?? Does any of them do a Fontana stain? Thanks From ethomas3224 <@t> comcast.net Thu Jan 14 13:07:04 2010 From: ethomas3224 <@t> comcast.net (ethomas3224@comcast.net) Date: Thu Jan 14 13:07:07 2010 Subject: [Histonet] Thermometer holder used with water bath Message-ID: <1838695753.10794371263496024645.JavaMail.root@sz0175a.westchester.pa.mail.comcast.net> Hi all, I like to know if anyone recall using in the past or present a small rubber suction cup that holds a thermometer approx. 4 in length at the bottom of?the glass insert of a?water bath. This accessory is approximately 1 inch in diameter and the thermometer slides across it. If you know the name and/or vendor, please let me know. Thanks Gina From kcai <@t> prosci-inc.com Thu Jan 14 13:29:54 2010 From: kcai <@t> prosci-inc.com (Karen Cai) Date: Thu Jan 14 13:29:57 2010 Subject: [Histonet] Eliminating the edge effect in IHC/IF In-Reply-To: <201001141011049169838@foxmail.com> Message-ID: <017f01ca954f$f3e9c7e0$9201a8c0@mic> Hi,=20 Thank you very much for your input. Could you describe more with the floating section method? I never did it before. =20 Best, Karen =20 -----Original Message----- From: TF [mailto:tifei@foxmail.com]=20 Sent: Wednesday, January 13, 2010 6:11 PM To: Rene J Buesa; histonet; Karen Cai Subject: Re: Re: [Histonet] Eliminating the edge effect in IHC/IF =20 Hi all i believe it is due to the wash + antibody accumulation problem happened between the edge of section and the adhesive slide. =20 Even you have circled the section with IHC pen(DAKO), and flattened all soultions evenly, it improved but did not disappear =20 if you stain the section using "floating section" method, no edge effect now. =20 =20 2010-01-14=20 _____ =20 TF=20 _____ =20 =B7=A2=BC=FE=C8=CB=A3=BA Rene J Buesa=20 =B7=A2=CB=CD=CA=B1=BC=E4=A3=BA 2010-01-12 13:30:49=20 =CA=D5=BC=FE=C8=CB=A3=BA histonet; Karen Cai=20 =B3=AD=CB=CD=A3=BA=20 =D6=F7=CC=E2=A3=BA Re: [Histonet] Eliminating the edge effect in IHC/IF=20 Usually that is the result of incomplete fixation. Check your fixation protocol. Ren=A8=A6 J. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =20 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Thu Jan 14 13:59:55 2010 From: foreightl <@t> gmail.com (Pat Laurie) Date: Thu Jan 14 14:00:01 2010 Subject: [Histonet] Fabric Softener sheets In-Reply-To: References: Message-ID: In my lab we have a couple of Techs that use the spray Static Guard, and sprays the microtome occasionally. I am not sure how well it works. It might freeze inside of the cryostat... On Wed, Jan 13, 2010 at 1:34 PM, wrote: > Hello all, > > > When using a dryer sheet in the cryostat to reduce static- > has anyone experienced an increased amount of artifact with fluorescein > stained slides??? > We will occasionally get a slide that has lots of fluorescent fibers all > over the section! > I vaguely remember being told that dryer sheets are not good for labs that > cut cryostat sections for either muscle histochemistries /or kidney > sections for immunofluorescent staining... > > I think using a small container of alcohol placed into the cryostat will > also help reduce static- > These dry months kill us!!!! > > Thanks in advance > > Theresa > Theresa R Dobersztyn HT (ASCP) > Senior Technologist-Histology/Electron Microscopy Labs > Dept. of Pathology and Laboratory Medicine > Phone: 330-543-8279 > Fax: 330-543-3226 > tdobersztyn@chmca.org > > > Akron Children's Hospital - Proud winner of the NorthCoast 99 "Best > Workplace" award! > > ************************************************************************* > This electronic mail transmission, including any attached files, may > contain confidential and/or privileged information for the sole use of the > intended recipient(s). It is not intended for transmission to, or receipt > by, any unauthorized parties. Any review, use, distribution, dissemination, > downloading, copying or disclosure by others is strictly > prohibited. If you are not the intended recipient (or authorized to receive > information for the intended recipient), you are hereby notified that you > have received this transmission in error. If you have received this > transmission in error, please delete it, as well as any copies, from your > system without copying it, and notify the sender by reply e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com From jtaylor <@t> meriter.com Thu Jan 14 14:36:53 2010 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu Jan 14 14:36:59 2010 Subject: [Histonet] CD15 clone Message-ID: <466B666475DE6547BBB0641E540A4BB506451DCD0A@EXVS1.meriter.com> Hi everyone, I'd like to hear what clone and company labs are using for their CD15 antibody. Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From amartinez <@t> carisdx.com Thu Jan 14 14:52:58 2010 From: amartinez <@t> carisdx.com (Martinez, Angela) Date: Thu Jan 14 14:52:56 2010 Subject: [Histonet] Unsubscribe Message-ID: Unsubscribe From LBenedetti <@t> leloir.org.ar Thu Jan 14 14:56:30 2010 From: LBenedetti <@t> leloir.org.ar (Lorena Benedetti) Date: Thu Jan 14 14:56:37 2010 Subject: [Histonet] Unsuscribe Message-ID: <0D913AE8FD19114CA45F61FD97636CD8D62A41@MUNICH> Unsuscribe From talulahgosh <@t> gmail.com Thu Jan 14 14:57:41 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Jan 14 14:57:47 2010 Subject: [Histonet] Unsubscribe In-Reply-To: References: Message-ID: By Zeus's beard, I cannot imagine why ANYONE is still writing unsubscribe to the list. USE THIS LINK. http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTE THAT IT IS AT THE BOTTOM OF EVERY EMAIL YOU SEND. EVEN THE ONES WITH THE WORD "unsubscribe" Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum On Thu, Jan 14, 2010 at 3:52 PM, Martinez, Angela wrote: > Unsubscribe > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Thu Jan 14 14:59:17 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Jan 14 14:59:22 2010 Subject: [Histonet] Unsuscribe In-Reply-To: <0D913AE8FD19114CA45F61FD97636CD8D62A41@MUNICH> References: <0D913AE8FD19114CA45F61FD97636CD8D62A41@MUNICH> Message-ID: MY BRAIN JUST EXPLODED. USE. THE. LINK. AT. THE. BOTTOM. OF. HISTONET. EMAILS. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum On Thu, Jan 14, 2010 at 3:56 PM, Lorena Benedetti wrote: > Unsuscribe > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From napoli <@t> siscom.net Thu Jan 14 15:18:02 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Thu Jan 14 15:18:07 2010 Subject: [Histonet] Dermpath Consultants Message-ID: <4b4f8a0a.12c.271e.279183691@siscom.net> In response to a question posed some time ago, if you are looking for a consultant to build a DERMPATH LAB, contact Andrew Burgeson, HTL @Buckeye Dermatology inc. Dermatopathologist/dermatologists and HISTOTECHS giving advice and guidance, as opposed to venture capitalists and non-medical third parties who won't be able to teach you the nuances of the trade. Contact BUCKEYEDERM@gmail.com or 513 680 1809. From scalligaris <@t> udd.cl Thu Jan 14 15:48:37 2010 From: scalligaris <@t> udd.cl (=?ISO-8859-1?Q?=22Sebasti=E1n_D._Calligaris=22?=) Date: Thu Jan 14 15:48:44 2010 Subject: [Histonet] Unsubscribe Message-ID: <0396DFB0-9DEC-421D-8FB7-08197D68F2FD@udd.cl> Unsubscribe -------------- next part -------------- Sebasti?n D. Calligaris, PhD Investigador Instituto de Ciencias Facultad de Medicina Cl?nica alemana-Universidad del Desarrollo From wdesalvo.cac <@t> hotmail.com Thu Jan 14 16:18:19 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu Jan 14 16:18:23 2010 Subject: [Histonet] Thermometer holder used with water bath In-Reply-To: <1838695753.10794371263496024645.JavaMail.root@sz0175a.westchester.pa.mail.comcast.net> References: <1838695753.10794371263496024645.JavaMail.root@sz0175a.westchester.pa.mail.comcast.net> Message-ID: I have not used the suction cup , but do have an inexpensive solution. Rubber washers. Select a two small rubber washers w/ an inside diameter slightly larger than the outside diameter of your thermometer and the outside diameter of the washer that provides approximately 3/4 inch distance from the inside opening. Remove a small portion of the edge to create a flattened section, slide the washers on each end and place in water bath. This method will elevate the thermometer from the bottom surface and the flattened edge will allow you to position so that you can easily read the thermometer. Simple, inexpensive and easily replaced. You should be able to find a washer to fit any size thermometer. William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 14 Jan 2010 19:07:04 +0000 > From: ethomas3224@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Thermometer holder used with water bath > > > > Hi all, > > > > I like to know if anyone recall using in the past or present a small rubber suction cup that holds a thermometer approx. 4 in length at the bottom of the glass insert of a water bath. This accessory is approximately 1 inch in diameter and the thermometer slides across it. > > If you know the name and/or vendor, please let me know. > > > > Thanks > > Gina > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/196390708/direct/01/ From tifei <@t> foxmail.com Thu Jan 14 20:26:45 2010 From: tifei <@t> foxmail.com (TF) Date: Thu Jan 14 20:27:04 2010 Subject: [Histonet] Eliminating the edge effect in IHC/IF References: <017f01ca954f$f3e9c7e0$9201a8c0@mic> Message-ID: <201001151026442343758@foxmail.com> c3RhaW5pbmcgd2FzIHBlcmZvcm1lZCBvbiBmbG9hdGluZyBzZWN0aW9ucy4uLmFuZCB0aHVzIHRo ZSBpbmN1YmF0aW9uIGNhbiByZWFjaCB0aGUgYm90aCBzaWRlcyBvZiBzZWN0aW9ucw0Kbm9ybWFs bHkgd2UgdXNlIDEyLzI0LzQ4IHdlbGwgcGxhdGUsIGFkZCAxIG1sLzAuNW1sLzAuMm1sIGFudGli b2R5IGRpbHV0ZWQgc29sdXRpb24gYW5kIHNoYWtlIA0KDQp3aWRlbHkgdXNlZCBmb3IgYnJhaW4g c2VjdGlvbiBzdGFpbmluZy4uLg0KDQp0aGUgd2FzaCB3aWxsIGJlIGJldHRlciwgaW4gdGhlIGVk Z2UgcGFydHMgb2Ygc2VjdGlvbnMNCg0KDQoyMDEwLTAxLTE1IA0KDQoNCg0KVEYgDQoNCg0KDQq3 orz+yMujuiBLYXJlbiBDYWkgDQq3osvNyrG85KO6IDIwMTAtMDEtMTUgIDAzOjI5OjU2IA0KytW8 /sjLo7ogdGlmZWlAZm94bWFpbC5jb207ICdSZW5lIEogQnVlc2EnOyAnaGlzdG9uZXQnIA0Ks63L zaO6IA0K1vfM4qO6IFJFOiBSZTogW0hpc3RvbmV0XSBFbGltaW5hdGluZyB0aGUgZWRnZSBlZmZl Y3QgaW4gSUhDL0lGIA0KIA0KSGksIA0KVGhhbmsgeW91IHZlcnkgbXVjaCBmb3IgeW91ciBpbnB1 dC4gQ291bGQgeW91IGRlc2NyaWJlIG1vcmUgd2l0aCB0aGUgZmxvYXRpbmcgc2VjdGlvbiBtZXRo b2Q/ICBJIG5ldmVyIGRpZCBpdCBiZWZvcmUuDQogDQpCZXN0LA0KS2FyZW4NCiANCi0tLS0tT3Jp Z2luYWwgTWVzc2FnZS0tLS0tDQpGcm9tOiBURiBbbWFpbHRvOnRpZmVpQGZveG1haWwuY29tXSAN ClNlbnQ6IFdlZG5lc2RheSwgSmFudWFyeSAxMywgMjAxMCA2OjExIFBNDQpUbzogUmVuZSBKIEJ1 ZXNhOyBoaXN0b25ldDsgS2FyZW4gQ2FpDQpTdWJqZWN0OiBSZTogUmU6IFtIaXN0b25ldF0gRWxp bWluYXRpbmcgdGhlIGVkZ2UgZWZmZWN0IGluIElIQy9JRg0KIA0KSGkgYWxsDQppIGJlbGlldmUg aXQgaXMgZHVlIHRvIHRoZSB3YXNoICsgYW50aWJvZHkgYWNjdW11bGF0aW9uIHByb2JsZW0gaGFw cGVuZWQgYmV0d2VlbiB0aGUgZWRnZSBvZiBzZWN0aW9uIGFuZCB0aGUgYWRoZXNpdmUgc2xpZGUu DQogDQpFdmVuIHlvdSBoYXZlIGNpcmNsZWQgdGhlIHNlY3Rpb24gd2l0aCBJSEMgcGVuKERBS08p LCBhbmQgZmxhdHRlbmVkIGFsbCBzb3VsdGlvbnMgZXZlbmx5LCBpdCBpbXByb3ZlZCBidXQgZGlk IG5vdCBkaXNhcHBlYXINCiANCmlmIHlvdSBzdGFpbiB0aGUgc2VjdGlvbiB1c2luZyAiZmxvYXRp bmcgc2VjdGlvbiIgbWV0aG9kLCBubyBlZGdlIGVmZmVjdCBub3cuDQogDQogDQoyMDEwLTAxLTE0 IA0KDQoNCg0KVEYgDQoNCg0KDQq3orz+yMujuiBSZW5lIEogQnVlc2EgDQq3osvNyrG85KO6IDIw MTAtMDEtMTIgIDEzOjMwOjQ5IA0KytW8/sjLo7ogaGlzdG9uZXQ7IEthcmVuIENhaSANCrOty82j uiANCtb3zOKjuiBSZTogW0hpc3RvbmV0XSBFbGltaW5hdGluZyB0aGUgZWRnZSBlZmZlY3QgaW4g SUhDL0lGIA0KVXN1YWxseSB0aGF0IGlzIHRoZSByZXN1bHQgb2YgaW5jb21wbGV0ZSBmaXhhdGlv bi4gQ2hlY2sgeW91ciBmaXhhdGlvbiBwcm90b2NvbC4NClJlbqimIEouDQotLS0gT24gTW9uLCAx LzExLzEwLCBLYXJlbiBDYWkgPGtjYWlAcHJvc2NpLWluYy5jb20+IHdyb3RlOg0KRnJvbTogS2Fy ZW4gQ2FpIDxrY2FpQHByb3NjaS1pbmMuY29tPg0KU3ViamVjdDogW0hpc3RvbmV0XSBFbGltaW5h dGluZyB0aGUgZWRnZSBlZmZlY3QgaW4gSUhDL0lGDQpUbzogaGlzdG9uZXRAbGlzdHMudXRzb3V0 aHdlc3Rlcm4uZWR1DQpEYXRlOiBNb25kYXksIEphbnVhcnkgMTEsIDIwMTAsIDI6MDAgUE0NCkhp LA0KSSBoYXZlIGEgcXVlc3Rpb24gZm9yIHRoZSBnZW5lcm91cyBpbnB1dC4gV2hlbiBJIGRvIHRo ZSBJSEMgb3IgSUYsIGl0DQpzZWVtcyB2ZXJ5IGNvbW1vbiB0aGF0IHRoZSBpbnRlbnNpdHkgb2Yg dGhlIGVkZ2UgYXJlYSBvZiB0aGUgdGlzc3VlIGlzDQphbHdheXMgc3Ryb25nZXIgdGhhbiB0aGUg Y2VudHJhbCB0aXNzdWUgcGFydC4gSXMgaXQgcG9zc2libGUgdG8NCmVsaW1pbmF0ZSB0aGlzIGFu ZCBtYWtlIHRoZSBzdGFpbmluZyBldmVubHkgZGlzdHJpYnV0ZWQgYXJvdW5kIHRoZSB3aG9sZQ0K dGlzc3VlIHNlY3Rpb24/DQpZb3VyIGtpbmQgaGVscCBpcyBncmVhdGx5IGFwcHJlY2lhdGVkLA0K VGhhbmtzIGluIGFkdmFuY2UsDQpCZXN0LA0KS2FyZW4NCkthcmVuIENhaQ0KUmVzZWFyY2ggU2Np ZW50aXN0DQpQcm9zY2kgSW5jb3Jwb3JhdGVkDQooODU4KSA1MTMtMjYzOCB4IDIwNA0KKDg1OCkg NTEzLTI2OTIgRmF4DQo8aHR0cDovL3d3dy5wcm9zY2ktaW5jLmNvbT4gd3d3LnByb3NjaS1pbmMu Y29tDQpfX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fXw0KSGlz dG9uZXQgbWFpbGluZyBsaXN0DQpIaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0 dHA6Ly9saXN0cy51dHNvdXRod2VzdGVybi5lZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0K ICAgICAgDQpfX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fXw0K SGlzdG9uZXQgbWFpbGluZyBsaXN0DQpIaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUN Cmh0dHA6Ly9saXN0cy51dHNvdXRod2VzdGVybi5lZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25l dA0K From V.Tilston <@t> liverpool.ac.uk Fri Jan 15 04:40:06 2010 From: V.Tilston <@t> liverpool.ac.uk (Tilston, Valerie) Date: Fri Jan 15 04:42:16 2010 Subject: [Histonet] Nuclei staining Message-ID: Hi, I'm trying to find a stain for nuclei only (without a high background stain) so that I am able to count the number of nuclei present in paraffin fixed livers. I appreciate that I could do DAPI immunofluorescence or IHC etc but does anyone know of any basic histology stains/protocols that give good results? I've already tried haematoxyin, nuclear fast red, carbol fuschin & Weigerts A+B..... Many thanks, Val From kmerriam2003 <@t> yahoo.com Fri Jan 15 08:09:51 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Jan 15 08:09:55 2010 Subject: [Histonet] requirements for certification In-Reply-To: <5F7CC9B788911848A79BC83453A3D6530360F0F208@Tomlinson.hti.com> References: <5F7CC9B788911848A79BC83453A3D6530360F0F208@Tomlinson.hti.com> Message-ID: <668773.31394.qm@web50305.mail.re2.yahoo.com> Hi Jen, I am HT certified and have spent my entire career working in research/veterinary pathology.? I received my HT?years ago when I was working for a?preclinical contract lab, I had one of our board-certified veterinary pathologists sign the form for me (I work for a biotech company now and did the same thing when I took my QIHC a couple of years ago).??It looks like you have the proper education and experience, I think all you?would need is for a board-certified pathologist?(either DVM or MD) to sign the form that they are familiar with your work and you should be good to go. ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Jennifer Anderson To: "histonet@lists.utsouthwestern.edu" Sent: Wed, January 13, 2010 4:50:53 PM Subject: [Histonet] requirements for certification Hello. I'm thinking about getting my HTL certification.? I've been a bench scientist in both academia and biotech for 21 years, which has included pre-clinical animal research and subsequent histological applications.? I've set up an histology lab and have a lot of IHC but only basic staining (mostly H&E), as well as loads of animal tissue processing and some human tissues.? I have a liberal arts degree (an AB) with a biology major and a chemistry minor.? We have both a DVM and a few MD's on site, but for pre-clinical and clinical research consult.? I would appreciate any insight on getting certified through an online program, how many hours is required, and what kind of mentorship is necessary. Thank you for your insights and time! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Fri Jan 15 08:29:44 2010 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Jan 15 08:29:57 2010 Subject: [Histonet] Unsubscribe In-Reply-To: <0396DFB0-9DEC-421D-8FB7-08197D68F2FD@udd.cl> References: <0396DFB0-9DEC-421D-8FB7-08197D68F2FD@udd.cl> Message-ID: <4B507BD8.5000908@umdnj.edu> > Unsubscribe Impossible... sorry, you're stuck on here FOREVER! ;) From carrolpb <@t> umdnj.edu Fri Jan 15 08:31:25 2010 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Jan 15 08:31:33 2010 Subject: [Histonet] Unsuscribe In-Reply-To: References: <0D913AE8FD19114CA45F61FD97636CD8D62A41@MUNICH> Message-ID: <4B507C3D.2050409@umdnj.edu> At least we've progressed from people asking to "unscribe"! Hahahaha... Emily Sours wrote: > MY BRAIN JUST EXPLODED. > USE. > THE. > LINK. > AT. > THE. > BOTTOM. > OF. > HISTONET. > EMAILS. > > Emily > > ...the thrill of being close to that hidden knowledge. That's the way I feel > when I read Nabokov. Encrypted within his words, encoded indecipherably, > ambiguously, is the equivalent of the secret of lightning. Something akin to > the secret code of higher human consciousness, the DNA, the genome of > genius. > -Ron Rosenbaum > > > On Thu, Jan 14, 2010 at 3:56 PM, Lorena Benedetti > wrote: > > >> Unsuscribe >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From cmiller <@t> physlab.com Fri Jan 15 09:05:47 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Jan 15 09:05:55 2010 Subject: [Histonet] Eosin evaporation. Message-ID: I recently purchased a leica refurbished autostainer, Before we hand stained our by hand. Now I notice the eosin is getting very low very fast, we have to replenish almost everyday. Before a bottle of Richard Allen Eosin would last the week or at least six days. Any idea's? Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Janice.Mahoney <@t> alegent.org Fri Jan 15 09:10:11 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Jan 15 09:10:26 2010 Subject: [Histonet] RE: Eosin evaporation. In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A55AF@EXCHMBC2.ad.ah.local> Hi Cheryl, Maybe the auto stainer does not drain the rack a well or it could just be a matter of the dipping and dunking that is causing more evaporation. Jan Omaha, N?E -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, January 15, 2010 9:06 AM To: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Eosin evaporation. I recently purchased a leica refurbished autostainer, Before we hand stained our by hand. Now I notice the eosin is getting very low very fast, we have to replenish almost everyday. Before a bottle of Richard Allen Eosin would last the week or at least six days. Any idea's? Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From sbreeden <@t> nmda.nmsu.edu Fri Jan 15 09:22:25 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jan 15 09:22:29 2010 Subject: [Histonet] RE: Eosin evaporation. In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A55AF@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A55AF@EXCHMBC2.ad.ah.local> Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46D9C@nmdamailsvr.nmda.ad.nmsu.edu> Eosin is alcohol-based, thus the evaporation. The containers on my autostainer get covered as soon as the last run is finished and that slows evaporation somewhat but I do replenish as needed. I like my eosin level in the container a little lower so we don't have pink-stained frosted ends, but that's just me! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From mdsmeltzer <@t> gmail.com Fri Jan 15 09:24:47 2010 From: mdsmeltzer <@t> gmail.com (Michael Smeltzer) Date: Fri Jan 15 09:24:54 2010 Subject: [Histonet] Fabric Softener sheets In-Reply-To: References: Message-ID: <9684e87c1001150724n71854c94jd1285e32267a8e91@mail.gmail.com> We placed a humidifier right next to our cryostat and kept movement by the cryostat to a minimum to preserve the "shroud of humidity". Worked like a charm when neither dryer sheets, grounding, a beaker of ethanol, or light breathing into the chamber helped. On Wed, Jan 13, 2010 at 4:34 PM, wrote: > Hello all, > > > When using a dryer sheet in the cryostat to reduce static- > has anyone experienced an increased amount of artifact with fluorescein > stained slides??? > We will occasionally get a slide that has lots of fluorescent fibers all > over the section! > I vaguely remember being told that dryer sheets are not good for labs that > cut cryostat sections for either muscle histochemistries /or kidney > sections for immunofluorescent staining... > > I think using a small container of alcohol placed into the cryostat will > also help reduce static- > These dry months kill us!!!! > > Thanks in advance > > Theresa > Theresa R Dobersztyn HT (ASCP) > Senior Technologist-Histology/Electron Microscopy Labs > Dept. of Pathology and Laboratory Medicine > Phone: 330-543-8279 > Fax: 330-543-3226 > tdobersztyn@chmca.org > > > Akron Children's Hospital - Proud winner of the NorthCoast 99 "Best > Workplace" award! > > ************************************************************************* > This electronic mail transmission, including any attached files, may > contain confidential and/or privileged information for the sole use of the > intended recipient(s). It is not intended for transmission to, or receipt > by, any unauthorized parties. Any review, use, distribution, dissemination, > downloading, copying or disclosure by others is strictly > prohibited. If you are not the intended recipient (or authorized to receive > information for the intended recipient), you are hereby notified that you > have received this transmission in error. If you have received this > transmission in error, please delete it, as well as any copies, from your > system without copying it, and notify the sender by reply e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MSHERWOOD <@t> PARTNERS.ORG Fri Jan 15 09:52:50 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Jan 15 09:52:55 2010 Subject: [Histonet] RE: Eosin evaporation. In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46D9C@nmdamailsvr.nmda.ad.nmsu.edu> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A55AF@EXCHMBC2.ad.ah.local> <4D14F0FC9316DD41972D5F03C070908B02E46D9C@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F3F@PHSXMB30.partners.org> I agree Sally; we cover as soon as the run is over and had to adjust the level of our H & E since the frosted end of the slides were getting stained as well. We also try to limit the sections to the bottom half or third of the sldie. Peg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, January 15, 2010 10:22 AM To: Mahoney,Janice A; Cheri Miller; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Eosin evaporation. Eosin is alcohol-based, thus the evaporation. The containers on my autostainer get covered as soon as the last run is finished and that slows evaporation somewhat but I do replenish as needed. I like my eosin level in the container a little lower so we don't have pink-stained frosted ends, but that's just me! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Kim <@t> ncpath.com Fri Jan 15 10:05:17 2010 From: Kim <@t> ncpath.com (Kimmie Rabe) Date: Fri Jan 15 10:04:34 2010 Subject: [Histonet] Experience with Tissue Tek VIP6 processor Message-ID: Our lab is in the market for a new processor. Does anyone have experience with the Tissue Tek VIP 6? And does anyone have experience using it xylene-free? Our histology supervisor would love to talk with you. Kim Rabe, M.D. North Central Pathology, P.A. From cmiller <@t> physlab.com Fri Jan 15 10:14:30 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Jan 15 10:14:36 2010 Subject: [Histonet] RE: Eosin evaporation. In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F3F@PHSXMB30.partners.org> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A55AF@EXCHMBC2.ad.ah.local> <4D14F0FC9316DD41972D5F03C070908B02E46D9C@nmdamailsvr.nmda.ad.nmsu.edu> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F3F@PHSXMB30.partners.org> Message-ID: We do this as well, that is why I am stumped. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] Sent: Friday, January 15, 2010 9:53 AM To: Breeden, Sara; Mahoney,Janice A; Cheri Miller; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Eosin evaporation. I agree Sally; we cover as soon as the run is over and had to adjust the level of our H & E since the frosted end of the slides were getting stained as well. We also try to limit the sections to the bottom half or third of the sldie. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From micropathlabs <@t> yahoo.com Fri Jan 15 10:22:32 2010 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Fri Jan 15 10:22:37 2010 Subject: [Histonet] RE: Eosin evaporation. In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A55AF@EXCHMBC2.ad.ah.local> <4D14F0FC9316DD41972D5F03C070908B02E46D9C@nmdamailsvr.nmda.ad.nmsu.edu> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F3F@PHSXMB30.partners.org> Message-ID: <368423.1101.qm@web57803.mail.re3.yahoo.com> Cheri, How close on your stain line is your eosin in relation to the oven? It's possible if it nearby, the oven is evaporating your eosin more rapidly than expected. Just a thought. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ________________________________ From: Cheri Miller To: "Sherwood, Margaret" ; "Breeden, Sara" ; "Mahoney, Janice A" ; histonet ; "histonet-bounces@lists.utsouthwestern.edu" Sent: Fri, January 15, 2010 11:14:30 AM Subject: RE: [Histonet] RE: Eosin evaporation. We do this as well, that is why I am stumped. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] Sent: Friday, January 15, 2010 9:53 AM To: Breeden, Sara; Mahoney,Janice A; Cheri Miller; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Eosin evaporation. I agree Sally; we cover as soon as the run is over and had to adjust the level of our H & E since the frosted end of the slides were getting stained as well. We also try to limit the sections to the bottom half or third of the sldie. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.? If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.? Any dissemination, distribution, or copying of this e-mail is strictly prohibited.? If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Fri Jan 15 10:26:25 2010 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Jan 15 10:29:09 2010 Subject: [Histonet] Eosin evaporation. In-Reply-To: <368423.1101.qm@web57803.mail.re3.yahoo.com> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A55AF@EXCHMBC2.ad.ah.local> <4D14F0FC9316DD41972D5F03C070908B02E46D9C@nmdamailsvr.nmda.ad.nmsu.edu> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F3F@PHSXMB30.partners.org> <368423.1101.qm@web57803.mail.re3.yahoo.com> Message-ID: <287069.77456.qm@web1112.biz.mail.sk1.yahoo.com> During the winter months the air is drier, especially with central heat. My tap water is sometimes as if it has been refrigerated too. Paula ________________________________ From: Sheila Haas To: Cheri Miller ; "Sherwood, Margaret" ; "Breeden, Sara" ; "Mahoney, Janice A" ; histonet ; "histonet-bounces@lists.utsouthwestern.edu" Sent: Fri, January 15, 2010 10:22:32 AM Subject: Re: [Histonet] RE: Eosin evaporation. Cheri, How close on your stain line is your eosin in relation to the oven? It's possible if it nearby, the oven is evaporating your eosin more rapidly than expected. Just a thought. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ________________________________ From: Cheri Miller To: "Sherwood, Margaret" ; "Breeden, Sara" ; "Mahoney, Janice A" ; histonet ; "histonet-bounces@lists.utsouthwestern.edu" Sent: Fri, January 15, 2010 11:14:30 AM Subject: RE: [Histonet] RE: Eosin evaporation. We do this as well, that is why I am stumped. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] Sent: Friday, January 15, 2010 9:53 AM To: Breeden, Sara; Mahoney,Janice A; Cheri Miller; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Eosin evaporation. I agree Sally; we cover as soon as the run is over and had to adjust the level of our H & E since the frosted end of the slides were getting stained as well. We also try to limit the sections to the bottom half or third of the sldie. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.? If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.? Any dissemination, distribution, or copying of this e-mail is strictly prohibited.? If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Fri Jan 15 09:09:56 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Jan 15 10:41:58 2010 Subject: [Histonet] Eosin evaporation. In-Reply-To: Message-ID: Are you keeping the lid to the stainer closed at all times, and are you covering each reagent container overnight? It should have come with a lid for each container. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Cheri Miller Sent: Friday, January 15, 2010 10:06 AM To: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Eosin evaporation. I recently purchased a leica refurbished autostainer, Before we hand stained our by hand. Now I notice the eosin is getting very low very fast, we have to replenish almost everyday. Before a bottle of Richard Allen Eosin would last the week or at least six days. Any idea's? Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From trathborne <@t> somerset-healthcare.com Fri Jan 15 10:42:34 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Jan 15 10:42:45 2010 Subject: [Histonet] MGMT mutation analysis Message-ID: We have a physician request for an MGMT mutation analysis on a paraffin block of a lung resection, previously diagnosed as malignant melanoma. I'm looking for a lab that performs this test. Reference labs feel free to reply. Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From jkiernan <@t> uwo.ca Fri Jan 15 10:58:27 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Jan 15 10:58:32 2010 Subject: [Histonet] Nuclei staining In-Reply-To: References: Message-ID: With the methods you list, you should easily be able to get selective nuclear staining with haemalum ("haematoxylin") or with Weigert's haematoxylin. The other traditional method, which is specific for DNA, is the Feulgen reaction. All these methods are in every book of staining techniques John Kiernan. Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Tilston, Valerie" Date: Friday, January 15, 2010 6:08 Subject: [Histonet] Nuclei staining To: "'histonet@lists.utsouthwestern.edu'" > Hi, > > I'm trying to find a stain for nuclei only (without a high > background stain) so that I am able to count the number of > nuclei present in paraffin fixed livers. I appreciate that > I could do DAPI immunofluorescence or IHC etc but does anyone > know of any basic histology stains/protocols that give good > results? I've already tried haematoxyin, nuclear fast red, > carbol fuschin & Weigerts A+B..... > > Many thanks, > > Val > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KMB01 <@t> grh.org Fri Jan 15 11:05:02 2010 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Fri Jan 15 11:06:52 2010 Subject: [Histonet] Experience with Tissue Tek VIP6 processor In-Reply-To: Message-ID: I would like that information also please. Kathy Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimmie Rabe Sent: Friday, January 15, 2010 8:05 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Experience with Tissue Tek VIP6 processor Our lab is in the market for a new processor. Does anyone have experience with the Tissue Tek VIP 6? And does anyone have experience using it xylene-free? Our histology supervisor would love to talk with you. Kim Rabe, M.D. North Central Pathology, P.A. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lhotaks <@t> mcmaster.ca Fri Jan 15 11:11:36 2010 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Fri Jan 15 11:11:58 2010 Subject: [Histonet] Mitochodrial IHC marker in FFPE skeletal muscle Message-ID: Hi all, I need to do a STAT3 IHC on mouse skeletal muscle. It should be showing up in mitochondria. Before purchasing the antibody I am wondering if the mitochodria are well enough preserved in FFPE tissue to show the distinct mitochondrial pattern. Is there a reliable mitochondrial "marker" (or a good mitochondrial protein antibody) that works on FFPE tissue? Thanks for your help, Sarka Lhotak McMaster university, Hamilton, Ontario From Lynne.Bell <@t> cvmc.org Fri Jan 15 11:34:28 2010 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Fri Jan 15 11:34:33 2010 Subject: [Histonet] RE: Experience with Tissue Tek VIP6 processor In-Reply-To: Message-ID: We just installed a Tissue Tek VIP6 processor. So far I love it! Please feel free to email me with any questions. I know that the Histonet is a great resource - they helped lead me to purchasing this unit. Lynne Lynne A. Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From rjbuesa <@t> yahoo.com Fri Jan 15 11:53:02 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 15 11:53:07 2010 Subject: [Histonet] Eosin evaporation. In-Reply-To: Message-ID: <724376.85230.qm@web65707.mail.ac4.yahoo.com> Before going home, cover the container Ren? J. --- On Fri, 1/15/10, Cheri Miller wrote: From: Cheri Miller Subject: [Histonet] Eosin evaporation. To: "histonet" , "histonet-bounces@lists..utsouthwestern.edu" Date: Friday, January 15, 2010, 10:05 AM I recently purchased a leica refurbished autostainer, Before we hand stained our by hand. Now I notice the eosin is getting very low very fast, we have to replenish almost everyday. Before a bottle of Richard Allen Eosin would last the week or at least six days. Any idea's? Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.? If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.? Any dissemination, distribution, or copying of this e-mail is strictly prohibited.? If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Fri Jan 15 12:04:08 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Jan 15 12:04:13 2010 Subject: [Histonet] re; eosin Message-ID: I have had some great suggestions from many of you, the problem is I am doing literally all of them. My question is on the Leica XL isn't the instrument supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why it is keeping a lot of carryover because it isn't vibrating. Also the alcohol after eosin is pretty pink after only a few racks. Tell me what you think.......thanks, Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rjbuesa <@t> yahoo.com Fri Jan 15 12:13:05 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 15 12:13:10 2010 Subject: [Histonet] re; eosin In-Reply-To: Message-ID: <869188.6516.qm@web65704.mail.ac4.yahoo.com> I think that you should talk with the person who sold you this refurbished instrument and present your problem. That will be more expeditious. Ren? J. --- On Fri, 1/15/10, Cheri Miller wrote: From: Cheri Miller Subject: [Histonet] re; eosin To: "histonet" Date: Friday, January 15, 2010, 1:04 PM I have had some great suggestions from many of you, the problem is I am doing literally all of them. My question is on the Leica XL isn't? the instrument supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why it is keeping a lot of carryover because it isn't vibrating. Also the alcohol after eosin is pretty pink after only a few racks. Tell me what you think.......thanks, Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.? If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.? Any dissemination, distribution, or copying of this e-mail is strictly prohibited.? If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Fri Jan 15 13:00:23 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Jan 15 13:00:29 2010 Subject: [Histonet] re; eosin In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F4A@PHSXMB30.partners.org> I never noticed if ours shakes to rid the excess, but I do know the 95% alcohol after eosin is pink but even when we stained manually, we had that as well. What we do with the XL is rotate the 2nd 95% to that position and put fresh 95% in the 2nd position. However, we only do that daily, not after each run. Even manually, we made that switch daily, not after each run. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, January 15, 2010 1:04 PM To: histonet Subject: [Histonet] re; eosin I have had some great suggestions from many of you, the problem is I am doing literally all of them. My question is on the Leica XL isn't the instrument supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why it is keeping a lot of carryover because it isn't vibrating. Also the alcohol after eosin is pretty pink after only a few racks. Tell me what you think.......thanks, Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From laurie.colbert <@t> huntingtonhospital.com Fri Jan 15 13:30:07 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Jan 15 13:30:12 2010 Subject: [Histonet] Lu Ann Aderson Message-ID: <57BE698966D5C54EAE8612E8941D768307AE40E4@EXCHANGE3.huntingtonhospital.com> LuAnn, If you are out there somewhere, would you mind giving me a call regarding a CJD case? If anyone knows how I can reach LuAnn, I would appreciate her email address or phone number. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 From lpjones <@t> srhs-pa.org Fri Jan 15 13:39:22 2010 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Fri Jan 15 13:39:25 2010 Subject: [Histonet] Poorly Designed Stain Bottles Message-ID: <4AE8039AEA096143B965CBC6D092166801F90F02F7@EXCH2007.srhs-pa.org> We have had continuing problems when pouring some stain reagents out of the gallon jugs in which they are sold. We have all ruined shoes and scrubs from the splashing as the stain comes out of the bottle. I thought I had seen some kind of no-splash spout adapter in the Marketlab catalog, but haven't been able to find anything. Does anyone know if there is such an adapter and/or where we can get one? Any suggestions or help appreciated. Thanks! Laura From kimtournear <@t> yahoo.com Fri Jan 15 13:47:05 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Jan 15 13:47:09 2010 Subject: [Histonet] MGMT mutation analysis In-Reply-To: Message-ID: <187073.60007.qm@web54208.mail.re2.yahoo.com> Contact Gwen at Lab Corp in Phoenix, AZ at 602454-8088...she should be able to help.... ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! --- On Fri, 1/15/10, Rathborne, Toni wrote: From: Rathborne, Toni Subject: [Histonet] MGMT mutation analysis To: histonet@lists.utsouthwestern.edu Date: Friday, January 15, 2010, 9:42 AM We have a physician request for an MGMT mutation analysis on a paraffin block of a lung resection, previously diagnosed as malignant melanoma. I'm looking for a lab that performs this test. Reference labs feel free to reply. Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Fri Jan 15 13:47:31 2010 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Jan 15 13:49:49 2010 Subject: [Histonet] Re: Mitochodrial IHC marker in FFPE skeletal muscle Message-ID: <11D9615B89C10747B1C985966A63D7CA2C434CE92F@KCL-MAIL04.kclad.ds.kcl.ac.uk> I have not yet found an anti mitochondrial Ab that works in mouse FFPE tissues, so I am very interested. However, the protein that Chemicon's MAB1273 is directed against survives....it has worked very well for me in human FFPE sections after Citric acid HIER ( see here for images: http://www.immunoportal.com/index.php ) At light microscopic level, no mitochondrial architecture is visible: rather, a series of dots are seen. STAT3 in mitochondria? Good luck. Sincerely, carl From histonet <@t> pathologyarts.com Fri Jan 15 13:58:17 2010 From: histonet <@t> pathologyarts.com (Histonet) Date: Fri Jan 15 13:54:44 2010 Subject: [Histonet] need tech with experience Message-ID: beautiful southern california with all our pollution and traffic has a potential opening for an experience tech at a high volume fast paced reference lab. we focus highly on quality and consistency and need to have people with the ability to cut and embed fast and still maintain a high level of quality. start time is approx. midnight, maybe as late as 2 a.m. we are currently working mon. through sat. so we might need a tue. through sat. schedule. reply if interested, curt From gayle.callis <@t> bresnan.net Fri Jan 15 14:51:32 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jan 15 14:51:41 2010 Subject: [Histonet] Re: VIP6 Processor Message-ID: <000601ca9624$85f8bae0$91ea30a0$@callis@bresnan.net> We have just replaced a VIP 1000 K series with a VIP6 and are absolutely delighted with the instrument. We had to shorten our routine tissue processing when over dehydration occurred, but this was due to the machine being more efficient compared to the older model. A minor adjustment only. We have not been disappointed, plus it is easy to operate, and has many excellent features (keeps records, 50 processing schedules possible). The latter has allowed each research laboratory to have their own processing schedule to fit their exact needs - custom processing at its best. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From gayle.callis <@t> bresnan.net Fri Jan 15 15:08:17 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jan 15 15:08:26 2010 Subject: [Histonet] RE: Dispensing stains from gallon jugs Message-ID: <000001ca9626$dd465c60$97d31520$@callis@bresnan.net> You wrote: We have had continuing problems when pouring some stain reagents out of the gallon jugs in which they are sold. We have all ruined shoes and scrubs from the splashing as the stain comes out of the bottle. ____________________________________________________ Not sure if you can find a spout adaptor. The ones I have used never work very well, still drip and are messy. You did not say if you use a funnel to help direct the reagent into the stain containers. Be sure it is a liquid funnel, one with tapered end as compared to a powder funnel (wide end for introducing powders/solids to containers). Liquid funnel should direct the stain without extra splashing, and introduce the stain to the side of the funnel.. You may want or might have a ring stand to hold the funnel. Some funnels have extra long ends. Also, there is an old trick taught in chemistry labs - use a very large serological pipette (25 or 50 ml size) and pour your stain so it flows down the pipette. Directing the flow into the container prevents splashing and spills. A plastic turkey baster found in any grocery store with a large diameter tube (remove the bulb) will also do the job - then wash and reuse. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From MadaryJ <@t> MedImmune.com Fri Jan 15 16:36:09 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Jan 15 16:36:15 2010 Subject: [Histonet] Leica 6 xylene free Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1301097996@MD1EV002.medimmune.com> Do you mean clearant free or xylene free? I use Hemo de and so far it has been fine. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From NKlemme <@t> sakuraus.com Fri Jan 15 17:33:15 2010 From: NKlemme <@t> sakuraus.com (Nancy Klemme) Date: Fri Jan 15 17:34:53 2010 Subject: [Histonet] RE: Experience with Tissue Tek VIP6 processor In-Reply-To: References: Message-ID: <782E3A02C2EB2347BEA6DEA69DC7AB8669D9A43C3F@sfamail.SAKURAUS.LOCAL> Hello, Dr. Rabe: I working on my 40th year as a histologist and remember hearing or reading of tissue processing by going from isopropanol directly into the paraffin step that had taken place in the 1950's or '60's. I'm not sending this as a declaration of my age, but, rather as a reference that xylene-free processing has been around for a long, long time, even though most of us used a clearing step between the dehydrant and the paraffin steps. ANY tissue processor (human or mechanical) can be used for this technique because the key elements are the chemicals that the tissues are being exposed to. Because attachments cannot be included, as a private eMail, I will send you a White Paper that Sakura published last year using this technique with the VIP6 that you're considering. Anyone reading this may obtain it from your Sakura Sales Manager or simply send me a return eMail. I'll also request to have it placed on our company web-site. As with any change in specimen handling procedure, you will want to document your validation of this technical change. Whatever instrument you ultimately choose to purchase, I send my kind regards. Nancy Klemme Edu. Svcs. Dir. / Sakura Finetek USA, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimmie Rabe Sent: Friday, January 15, 2010 8:05 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Experience with Tissue Tek VIP6 processor Our lab is in the market for a new processor. Does anyone have experience with the Tissue Tek VIP 6? And does anyone have experience using it xylene-free? Our histology supervisor would love to talk with you. Kim Rabe, M.D. North Central Pathology, P.A. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yan.liang <@t> mdsinc.com Fri Jan 15 17:47:38 2010 From: yan.liang <@t> mdsinc.com (Liang, Yan) Date: Fri Jan 15 17:48:28 2010 Subject: [Histonet] Golder's trichrome stain Message-ID: <42D66A529F6965498107BC4A6EF63FC70400D7E1@CATOM-MDMAPUWDE.mds.mdsinc.com> We have trouble to stain bone paraffin section with Golder's trichrome stain. Our lab use stain for over ten years for plastic and paraffin section on bone. The stain is stable. Only this time, our some mineralized bone of the section was stained with red and green color mix. We checked reagents and changed every reagent, prepared new solutions, still the same. Does anyone have experience with the red bone stain? Thank you Yan Liang MD PhD MDS Pharma This e-mail and any files transmitted with it may contain privileged and/or confidential information and may be read or used only by the intended recipient. If you are not the intended recipient of the e-mail or any of its attachments, please be advised that you have received this e-mail in error and any use, dissemination, distribution, forwarding, printing or copying of this e-mail or any attached files is strictly prohibited. If you have received this e-mail in error, please immediately purge it and all attachments and notify the sender by reply e-mail or contact the sender at the number listed. From tkngflght <@t> yahoo.com Sat Jan 16 08:06:14 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sat Jan 16 08:06:24 2010 Subject: [Histonet] top off eosin with alcohol Message-ID: <340144.66618.qm@web50901.mail.re2.yahoo.com> when eosin evaporates, the dye concentration increases and adding more eosin will increase your stain strength. Top with a good quality (not recycled) alcohol to maintain the level.? ? This is assuming the level drops due to evaporation, not carry-over. ? Cheryl ? ? From pathmaster <@t> yahoo.com Sat Jan 16 14:50:07 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Sat Jan 16 14:50:11 2010 Subject: [Histonet] (no subject) Message-ID: <755228.93996.qm@web111115.mail.gq1.yahoo.com> Cheryl, You can set the length of time the slide rack remains over the stating dish to drain before moving on, also I believe there is also a shake function to aid in preventing carryover. ? I've also noticed on my XL that the eosin evaporates faster that any of the other dishes. We just top off with 95% as needed. ? Jeff Silverman Southside Hopsital Bay Shore, New York From amosbrooks <@t> gmail.com Sat Jan 16 22:23:19 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Jan 16 22:23:25 2010 Subject: [Histonet] Re: Mitochodrial IHC marker in FFPE skeletal Message-ID: <582736991001162023n242fedcaia9bd7782396bd18c@mail.gmail.com> Hi, Regarding mitochondrial IHC SDHB works well in paraffin sections. I used the one from AbCam. Amos Date: Fri, 15 Jan 2010 19:47:31 +0000 From: "Hobbs, Carl" Subject: [Histonet] Re: Mitochodrial IHC marker in FFPE skeletal muscle To: "histonet@lists.utsouthwestern.edu" Message-ID: < 11D9615B89C10747B1C985966A63D7CA2C434CE92F@KCL-MAIL04.kclad.ds.kcl.ac.uk> Content-Type: text/plain; charset="us-ascii" I have not yet found an anti mitochondrial Ab that works in mouse FFPE tissues, so I am very interested. However, the protein that Chemicon's MAB1273 is directed against survives....it has worked very well for me in human FFPE sections after Citric acid HIER ( see here for images: http://www.immunoportal.com/index.php ) At light microscopic level, no mitochondrial architecture is visible: rather, a series of dots are seen. STAT3 in mitochondria? Good luck. Sincerely, carl From Ken_Marissael <@t> vwr.com Sun Jan 17 15:06:29 2010 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Sun Jan 17 15:06:35 2010 Subject: [Histonet] Ken Marissael/VWRI is at the VWR National Sales Meeting Message-ID: I will be out of the office starting 01/16/2010 and will not return until 01/21/2010. I will be away at the VWR National Sales Meeting beginning Monday 1/18 and will return on Thursday 1/21. I will have my Blackberry, and access to e-mail in the evenings. If you need immediate help, please contact Customer Service at 1-800-932-5000. From tifei <@t> foxmail.com Sun Jan 17 20:41:40 2010 From: tifei <@t> foxmail.com (TF) Date: Sun Jan 17 20:41:53 2010 Subject: [Histonet] 0.1 M PB & 0.01M PBS Message-ID: <201001181041396453729@foxmail.com> We use 0.1 PB for tissue fixation (4% PFA), cryo-preservation (30% sucrose) then go 0.01 PBS for IHC though these are routines here, I wonder anyone can specify on the use of two different solutions, especially why? 2010-01-18 TF From anonwums1 <@t> gmail.com Sun Jan 17 21:02:22 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Sun Jan 17 21:02:27 2010 Subject: [Histonet] 0.1 M PB & 0.01M PBS In-Reply-To: <201001181041396453729@foxmail.com> References: <201001181041396453729@foxmail.com> Message-ID: <858249121001171902p22dde563m64e30605451d0aa7@mail.gmail.com> I've used 1X PBS for everything and it seems to work. Adam On Sun, Jan 17, 2010 at 8:41 PM, TF wrote: > We use 0.1 PB for tissue fixation (4% PFA), cryo-preservation (30% sucrose) > then go 0.01 PBS for IHC > > though these are routines here, I wonder anyone can specify on the use of > two different solutions, especially why? > > > 2010-01-18 > > > > TF > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tifei <@t> foxmail.com Sun Jan 17 21:08:30 2010 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Sun Jan 17 21:23:02 2010 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIDAuMSBNIFBCICYgMC4wMU0gUEJT?= References: <201001181041396453729@foxmail.com>, <858249121001171902p22dde563m64e30605451d0aa7@mail.gmail.com> Message-ID: <201001181108201852066@foxmail.com> For longer term preservation of brain sectins in floating state...0.01M PBS is not as good as 0.1 M PB.. 2010-01-18 TF ???? Adam . ????? 2010-01-18 11:02:26 ???? tifei ??? Histonet@lists.utsouthwestern.edu ??? Re: [Histonet] 0.1 M PB & 0.01M PBS I've used 1X PBS for everything and it seems to work. Adam On Sun, Jan 17, 2010 at 8:41 PM, TF wrote: We use 0.1 PB for tissue fixation (4% PFA), cryo-preservation (30% sucrose) then go 0.01 PBS for IHC though these are routines here, I wonder anyone can specify on the use of two different solutions, especially why? 2010-01-18 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjkitten <@t> live.com Mon Jan 18 06:45:15 2010 From: sjkitten <@t> live.com (S R) Date: Mon Jan 18 06:45:24 2010 Subject: [Histonet] Formaldehyde Testing Message-ID: Happy Monday Histonetters: I am trying to find a source that tells you how often you should be testing formaldehyde and xylene. Thanks in advanced Sammy _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/196390710/direct/01/ From thecitan <@t> yahoo.com Mon Jan 18 07:01:56 2010 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Mon Jan 18 07:02:03 2010 Subject: [Histonet] Tissue fall off and small specimen "cooked" Message-ID: <140126781-1263819717-cardhu_decombobulator_blackberry.rim.net-27856769-@bda950.bisx.prod.on.blackberry> I've had a strange occurrence in my derm lab. with all procedures staying the same (processor chemicals and autostainers have not been rotated yet) my tissue keeps falling off the slides. This is happening more to the smaller specimens. Also the doctors are mentioning that the smaller specimens look "cooked" which I'm guessing is shrinkage. I have a few theories and am going to test a few things - but I wanted to see if anyone out there had any suggestions as to the cause. Any suggestions would be appreciated! Thanks. Sent from my Verizon Wireless BlackBerry From chesarato <@t> hotmail.com Mon Jan 18 08:04:14 2010 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Mon Jan 18 08:05:29 2010 Subject: [Histonet] How Have You Been Lately? Message-ID: Hello Dear, How are you doing these days? You know, I bought one new pair of Nike shoes on one great online shop www.OkIsell.com. They are so amazing! They provide a lot of other brand new casual shoes, sport shoes, high heels, ugg boots, such as Prada, Christian Louboutin, Gucci, Lascote, Puma, and so on. The styles are the most popular ones for the 2010 year. Have to say it is a great and specialize online shoes store. Hope you can find some great things there too www.OkIsell.com.! Give my regards to your family then _________________________________________________________________ ?Quer?s chatear en todos lados con tu celu? ?Registrate a SMS Messenger! http://www.somosmessengersiempre.com/?ocid=TWLH From cmiller <@t> physlab.com Mon Jan 18 08:28:30 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Jan 18 08:28:37 2010 Subject: [Histonet] re; eosin Message-ID: Thanks for all your help. It makes sense that it would evaporate faster on the Leica as opposed to hand staining. The eosin on the Leica is uncovered for 6 plus hours a day when hand staining it was covered except when we were staining a rack. I will add 100 alcohol as needed. Again thanks for all your help. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cmiller <@t> physlab.com Mon Jan 18 08:33:32 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Jan 18 08:33:40 2010 Subject: [Histonet] CAP regs Message-ID: Does CAP require an ASCP certification for a supervisor or manager to run a lab or would a BA in science be accepted. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From mpence <@t> grhs.net Mon Jan 18 08:37:09 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jan 18 08:37:15 2010 Subject: [Histonet] top off eosin with alcohol In-Reply-To: <340144.66618.qm@web50901.mail.re2.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D09@is-e2k3.grhs.net> I always top with the next alcohol in the line and then rotate the alcohols. This refreshes the eosin and gives you a cleaner line. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Saturday, January 16, 2010 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] top off eosin with alcohol when eosin evaporates, the dye concentration increases and adding more eosin will increase your stain strength. Top with a good quality (not recycled) alcohol to maintain the level.? ? This is assuming the level drops due to evaporation, not carry-over. ? Cheryl ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgribbins <@t> wittenberg.edu Mon Jan 18 09:58:34 2010 From: kgribbins <@t> wittenberg.edu (Kevin M. Gribbins) Date: Mon Jan 18 09:58:42 2010 Subject: [Histonet] SPI Long Knife Maker Manual Message-ID: Does anyone have a manual or know where I could get a hold of a manual that shows how to make glass knifes using a SPI long knife maker. Thanks, Kevin Gribbins, Ph.D. Associate Professor Biology Department Wittenberg University PO Box 720 Springfield OH, 45501-0720 Phone: 937-327-6478 Fax: 937-327-6487 email: kgribbins@wittenberg.edu From DKBoyd <@t> chs.net Mon Jan 18 10:59:10 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Mon Jan 18 10:58:35 2010 Subject: [Histonet] CAP question Message-ID: We are no longer CAP inspected but Joint Commission. Could someone tell me what the CAP standard for breast biopsies states about formalin fixation? ie: 24 hrs, 36 hrs etc. Thanks. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From rmweber113 <@t> comcast.net Mon Jan 18 11:11:23 2010 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Mon Jan 18 11:11:25 2010 Subject: [Histonet] fumehood In-Reply-To: <2024095019.387271263833796168.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Message-ID: <1536325487.394651263834683174.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Hi,??? Does anyone know a good tabletop fumehood for grossing with formalin??? I was thinking of one with 60f/sec to 110f/sec.? Does that meet the regulations? Marilynn Weber H.T.(ASCP)QIHC From cliffberger <@t> mac.com Mon Jan 18 11:39:33 2010 From: cliffberger <@t> mac.com (Cliff Berger) Date: Mon Jan 18 11:39:41 2010 Subject: [Histonet] fumehood In-Reply-To: <1536325487.394651263834683174.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Message-ID: You should look at: http://www.airfiltronix.com/ On 1/18/10 12:11 PM, "rmweber113@comcast.net" wrote: > > Hi,??? Does anyone know a good tabletop fumehood for grossing with formalin??? > I was thinking of one with 60f/sec to 110f/sec.? Does that meet the > regulations? > > Marilynn Weber H.T.(ASCP)QIHC > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Mon Jan 18 11:49:27 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jan 18 11:49:31 2010 Subject: [Histonet] CAP question In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D0C@is-e2k3.grhs.net> Here it is from CAP: ANP.22998 Phase I N/A YES NO If the laboratory assesses HER2 protein over-expression by immunohistochemistry (IHC) or HER2 gene amplification by fluorescence in situ hybridization (FISH), does the laboratory have a documented procedure for ensuring appropriate length of fixation of specimens tested? NOTE: Specimens subject to HER2 testing should be fixed in 10% neutral buffered formalin for at least 6 hours and no longer than 48 hours. While core biopsies must not be fixed for less than 1 hour, it is recommended that such specimens have the same fixation as larger specimens (i.e., 6 hours minimum). It is recommended that time of fixation be recorded and included in the report if available. While the maximum fixation time of 48 hours is not an exclusion criterion for HER2 testing, laboratories should qualify any negative results for specimens fixed longer than 48 hours. For cases with negative results by IHC, consideration should be given to performing confirmatory analysis by FISH. Laboratories testing specimens obtained from another institution should have a policy that addresses time of fixation. Information on time of fixation may be obtained by appropriate questions on the laboratory's requisition form. The time of fixation should be recorded in the final report. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: Monday, January 18, 2010 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question We are no longer CAP inspected but Joint Commission. Could someone tell me what the CAP standard for breast biopsies states about formalin fixation? ie: 24 hrs, 36 hrs etc. Thanks. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Albert.Santiago <@t> uphs.upenn.edu Mon Jan 18 12:22:20 2010 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Mon Jan 18 12:22:26 2010 Subject: [Histonet] job openings Message-ID: Hello fellow histonetters. Our derm lab in Philadelphia (Univ.of Pennsylvania.Hosp.) is in search of a few good histotechs. If interested, please contact me for detailed information. Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6008/6539-office 215-662-6150-fax The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From laurie.colbert <@t> huntingtonhospital.com Mon Jan 18 13:25:27 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Jan 18 13:25:31 2010 Subject: [Histonet] Teleconferences Message-ID: <57BE698966D5C54EAE8612E8941D768307CD8716@EXCHANGE3.huntingtonhospital.com> Is there anyone in the Pasadena, CA area that will be showing the NSH teleconferences at their facility this year and would be willing to allow some of our employees to come watch them to receive CEU's? Thank you, Laurie Colbert Huntington Hospital Pasadena, CA From gvdobbin <@t> ihis.org Mon Jan 18 13:29:06 2010 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Jan 18 13:29:26 2010 Subject: [Histonet] Advice from Canadian Labs Message-ID: Hello Canadian Colleagues, I am wondering what other clinical institutions in Canada are doing with regard to retention of hardcopy consult reports. Here in prince Edward Island we now have an electronic patient health record province-wide so we no longer have hardcopy surgical reports filed in the lab. Consult reports received from reference laboratories are being scanned into the patient's report and checked and verified for ledgibility. Any section that does not scan clear enough to read easily is edited to match the hardcopy. So in our lab, the patient report and any associated consult reports will be stored indefinately electronically. The CAP guidelines (which we refer to but are not held to) suggest "Surgical Consultation" reports should be kept indefinitely. I think therefore, we are meeting the expectation here, but how long should I retain the hardcopy of these consultation reports, 2 years? 20 years?? What are others doing in this regard? Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From arvidsonkristen <@t> yahoo.com Mon Jan 18 13:39:40 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Mon Jan 18 13:39:44 2010 Subject: [Histonet] Leica Special Stainer Message-ID: <248791.39720.qm@web65716.mail.ac4.yahoo.com> Has anyone used the Leica Special Stainer? From mucram11 <@t> comcast.net Mon Jan 18 14:00:15 2010 From: mucram11 <@t> comcast.net (mucram11@comcast.net) Date: Mon Jan 18 14:00:26 2010 Subject: [Histonet] Advice from Canadian Labs In-Reply-To: References: Message-ID: <404206507-1263844816-cardhu_decombobulator_blackberry.rim.net-1341861708-@bda860.bisx.prod.on.blackberry> It is s good question and since we are all moving that way I hope any advanced answers will be posted to all of us. PEI is a beautiful place!! Pam Marcum UAMS Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Greg Dobbin" Date: Mon, 18 Jan 2010 15:29:06 To: Subject: [Histonet] Advice from Canadian Labs Hello Canadian Colleagues, I am wondering what other clinical institutions in Canada are doing with regard to retention of hardcopy consult reports. Here in prince Edward Island we now have an electronic patient health record province-wide so we no longer have hardcopy surgical reports filed in the lab. Consult reports received from reference laboratories are being scanned into the patient's report and checked and verified for ledgibility. Any section that does not scan clear enough to read easily is edited to match the hardcopy. So in our lab, the patient report and any associated consult reports will be stored indefinately electronically. The CAP guidelines (which we refer to but are not held to) suggest "Surgical Consultation" reports should be kept indefinitely. I think therefore, we are meeting the expectation here, but how long should I retain the hardcopy of these consultation reports, 2 years? 20 years?? What are others doing in this regard? Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathmaster <@t> yahoo.com Mon Jan 18 16:18:43 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Mon Jan 18 16:18:47 2010 Subject: [Histonet] CAP standard for formalin and xyelene testing Message-ID: <354373.98171.qm@web111112.mail.gq1.yahoo.com> CAP standard says formaldehyde testing should be done for each employee as an eight hour exposure and a short term exposure limit. If two tests done at least one week apart are under action levels, there is no need for further ongoing monitoring unless new process, employee, or other factor that might increase exposure is introduced. Or if an employee feels they have a problem and requests it. So I guess each employee is tested twice and then that's it unless you over the limit. ? Xylene is similar- once you have two tests under action levels, there is no need for ongoing monitoring unless things change as above. ? Jeffrey Silverman HT HTL QIHC (ASCP) Pathologists' Assistant- Lab Safety Officer Southside Hospital NSLIJHS ?Bay Shore, NY USA From saby_joseph_a <@t> yahoo.com Mon Jan 18 16:33:39 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Mon Jan 18 16:33:47 2010 Subject: [Histonet] (no subject) Message-ID: <51989.86902.qm@web113810.mail.gq1.yahoo.com> Robert- The artifact you describe is almost always due to varying combinations of?two issues: ??? 1) overprocessing the biopsies, making them tough, and ??? 2) too agressive facing of the blocks.? Thick facing sections cause cracks deep in the tough tissue. I have worked through these issues in the past, but patience is required.? Soak and chill the blocks repeatedly while facing in at normal sectioning thickness.? You will need to work through the area of the biopsies that have the cracks forced into their structure.? With luck, you will have enough good tissue deeper in the block to provide a good section. Good luck! Joe Saby, BA HT ________________________________ From: "Moody, Robert" To: "histonet@lists.utsouthwestern.edu" Sent: Wed, January 13, 2010 11:08:53 PM Subject: [Histonet] RE: Histonet Digest, Vol 74, Issue 12 Hi, All we are having problems with chatter in our biopsies their usually on the edge of tissue is the a problem with the cutting or in the handling of the tissue after it is removed from the patient like the biopsies being left out to dry or not put in formalin what are some of your experience with this.. Robert Moody HT ASCP From alisha <@t> ka-recruiting.com Mon Jan 18 18:09:50 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Mon Jan 18 18:09:09 2010 Subject: [Histonet] Histology Supervisor Job Opportunity Message-ID: <1355811554.1263859790030.JavaMail.cfservice@webserver60> Dear Histonet Subscribers, Would you consider relocating if the employer were willing to pay for your relocation? Are you interested in learning about other job opportunities for free? Are you interested in learning what other laboratory professionals are making in different areas of the country? Are you looking for better hours, or higher pay, or to be given the opportunity to work your way up the career ladder? If you answered yes to any of these questions, please contact me to learn about other career opportunities. I am the founder of K.A. Recruiting, a healthcare recruiting company that specializes in your industry. I have clients across the United States that hire me to find them talented lab professionals, like yourself. My service is completely FREE to the job seeker. Not only is my service free, I will also be able to tell you about "hidden jobs" that may never be posted on websites and I will be able to guide you every step of the way through the interview process (helping you to secure the job of your dreams and assist with the salary negotiation process, ensuring that you will be paid the highest rate possible for your experience level). Please take a moment to read through the highlighted opportunity below, as well as the other job opportunities I am working on currently on. Highlighted Job Opportunity! One particular client I am working with is looking for a Histology Supervisor for a lab in Las Vegas, NV. This lab is looking for someone with experience, HT(ASCP) or HTL(ASCP) certified, and someone who either lives in or is willing to relocate to Las Vegas, NV. Las Vegas, Nevada is perhaps one of the most exciting places in the entire country. While most people consider the casino portal to be more of a tourism capital than a long term residency, there are plenty of people who have found an excellent home in this city. Sunshine days usually occur 300 days out of the year, and there are very little rainy days. My client is offering an excellent compensation package, relocation assistance, and full benefits. Below is a list of some of the other great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Opportunities: Histotechs/Cytotechs CT - Histology Operations Manager Southern CA - Histology Supervisor New York City - Surgical Pathology and Histology Supervisor New York City - Histotech 3rd shift Las Vegas, NV - Histotech 3rd shift GA - Histotech 1st shift OK - Histotech 1st shift (with opportunity to be promoted to supervisor) Long Island, NY - Cytotech PA - Cytology Supervisor CT - Cytotech Palm Springs, CA - Histotech - 1st shift Pathologist's Assistant NV - Pathologist's Assistant 2nd shift If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Happy 2010! Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com * Please note change in address and phone number From kmerriam2003 <@t> yahoo.com Tue Jan 19 07:04:55 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Jan 19 07:05:00 2010 Subject: [Histonet] fumehood In-Reply-To: <1536325487.394651263834683174.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> References: <1536325487.394651263834683174.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Message-ID: <882246.98033.qm@web50302.mail.re2.yahoo.com> Surgipath makes a nice tabletop?fume hood,?we have one in our lab and?it?works quite well. http://www.surgipath.com/us-en/Products/equipment-accessories/fume-hood Good luck! Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "rmweber113@comcast.net" To: histonet@lists.utsouthwestern.edu Sent: Mon, January 18, 2010 12:11:23 PM Subject: [Histonet] fumehood Hi,??? Does anyone know a good tabletop fumehood for grossing with formalin??? I was thinking of one with 60f/sec to 110f/sec.? Does that meet the regulations? Marilynn Weber H.T.(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Tue Jan 19 08:15:39 2010 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Jan 19 08:16:03 2010 Subject: [Histonet] Advice from Canadian Labs Message-ID: Hey Pam, No problem, I will. Nice to hear someone south of our border has heard of Canada's smallest province!! :-) Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> 1/18/2010 4:00 PM >>> It is s good question and since we are all moving that way I hope any advanced answers will be posted to all of us. PEI is a beautiful place!! Pam Marcum UAMS Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Greg Dobbin" Date: Mon, 18 Jan 2010 15:29:06 To: Subject: [Histonet] Advice from Canadian Labs Hello Canadian Colleagues, I am wondering what other clinical institutions in Canada are doing with regard to retention of hardcopy consult reports. Here in prince Edward Island we now have an electronic patient health record province-wide so we no longer have hardcopy surgical reports filed in the lab. Consult reports received from reference laboratories are being scanned into the patient's report and checked and verified for ledgibility. Any section that does not scan clear enough to read easily is edited to match the hardcopy. So in our lab, the patient report and any associated consult reports will be stored indefinately electronically. The CAP guidelines (which we refer to but are not held to) suggest "Surgical Consultation" reports should be kept indefinitely. I think therefore, we are meeting the expectation here, but how long should I retain the hardcopy of these consultation reports, 2 years? 20 years?? What are others doing in this regard? Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From Janice.Mahoney <@t> alegent.org Tue Jan 19 08:15:51 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Jan 19 08:16:31 2010 Subject: [Histonet] CAP standard for formalin and xyelene testing In-Reply-To: <354373.98171.qm@web111112.mail.gq1.yahoo.com> References: <354373.98171.qm@web111112.mail.gq1.yahoo.com> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A55C9@EXCHMBC2.ad.ah.local> My understanding is that it is the job, not the employee that must be monitored. So if you have three people using the same process of changing the stainer, only one of those people needs to be tested. Please correct me if I am wrong. Thanks, Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Monday, January 18, 2010 4:19 PM To: sjkitten@live.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP standard for formalin and xyelene testing CAP standard says formaldehyde testing should be done for each employee as an eight hour exposure and a short term exposure limit. If two tests done at least one week apart are under action levels, there is no need for further ongoing monitoring unless new process, employee, or other factor that might increase exposure is introduced. Or if an employee feels they have a problem and requests it. So I guess each employee is tested twice and then that's it unless you over the limit. Xylene is similar- once you have two tests under action levels, there is no need for ongoing monitoring unless things change as above. Jeffrey Silverman HT HTL QIHC (ASCP) Pathologists' Assistant- Lab Safety Officer Southside Hospital NSLIJHS Bay Shore, NY USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From KPercival <@t> wyeth.com Tue Jan 19 08:47:30 2010 From: KPercival <@t> wyeth.com (Percival Karen) Date: Tue Jan 19 08:47:38 2010 Subject: [Histonet] CAP standard for formalin and xyelene testing In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A55C9@EXCHMBC2.ad.ah.local> References: <354373.98171.qm@web111112.mail.gq1.yahoo.com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A55C9@EXCHMBC2.ad.ah.local> Message-ID: <4B557FB202000011001F7C27@gv01a67m.gv.us.pri.wyeth.com> Some time ago, I had tested each one of my staff doing the same procedures for both formaldehyde and xylene. Procedures included, autostaining, processor changes, staining rack changes, recycling, and disposal procedures. Everyone except for one person was within normal limits. This one person was tested several times over, and his/her results continued to be outside of the acceptable range for both long and short-term exposure limits. Finally, we had to physically observe this person doing his/her job for both the short and long-term exposure tests. Long story short, this person was simply working too quickly and was not being as cautious as s/he should have been. Splashing occurred all too frequently. Although s/he followed the same procedures and protocols for all of his/her activities as the other staff members, his/her level of carelessness caused his/her monitor badge readings to constantly be above the acceptable limits. Test everyone. Karen Percival, BS, HT Research Scientist II Pfizer Research DSRD 1 Burtt Road G3025 Andover, MA 01810 888-577-1500 x 4058 kpercival @wyeth.com >>> "Mahoney,Janice A" 1/19/2010 9:15 AM >>> My understanding is that it is the job, not the employee that must be monitored. So if you have three people using the same process of changing the stainer, only one of those people needs to be tested. Please correct me if I am wrong. Thanks, Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Monday, January 18, 2010 4:19 PM To: sjkitten@live.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP standard for formalin and xyelene testing CAP standard says formaldehyde testing should be done for each employee as an eight hour exposure and a short term exposure limit. If two tests done at least one week apart are under action levels, there is no need for further ongoing monitoring unless new process, employee, or other factor that might increase exposure is introduced. Or if an employee feels they have a problem and requests it. So I guess each employee is tested twice and then that's it unless you over the limit. Xylene is similar- once you have two tests under action levels, there is no need for ongoing monitoring unless things change as above. Jeffrey Silverman HT HTL QIHC (ASCP) Pathologists' Assistant- Lab Safety Officer Southside Hospital NSLIJHS Bay Shore, NY USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Tue Jan 19 08:55:35 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Jan 19 08:55:55 2010 Subject: [Histonet] CAP standard for formalin and xyelene testing In-Reply-To: <4B557FB202000011001F7C27@gv01a67m.gv.us.pri.wyeth.com> Message-ID: Jan, You are correct. It is the task. Must like when doing proficiency testing, rotate employees on all of these task. While I agree with Karen that it is probably best practice to do all of them, it is not required. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Percival Karen" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/19/2010 08:47 AM To "Mahoney Janice A" , "sjkitten@live.com" , "Silverman' 'Jeffrey" cc "histonet@lists.utsouthwestern.edu" Subject RE: [Histonet] CAP standard for formalin and xyelene testing Some time ago, I had tested each one of my staff doing the same procedures for both formaldehyde and xylene. Procedures included, autostaining, processor changes, staining rack changes, recycling, and disposal procedures. Everyone except for one person was within normal limits. This one person was tested several times over, and his/her results continued to be outside of the acceptable range for both long and short-term exposure limits. Finally, we had to physically observe this person doing his/her job for both the short and long-term exposure tests. Long story short, this person was simply working too quickly and was not being as cautious as s/he should have been. Splashing occurred all too frequently. Although s/he followed the same procedures and protocols for all of his/her activities as the other staff members, his/her level of carelessness caused his/her monitor badge readings to constantly be above the acceptable limits. Test everyone. Karen Percival, BS, HT Research Scientist II Pfizer Research DSRD 1 Burtt Road G3025 Andover, MA 01810 888-577-1500 x 4058 kpercival @wyeth.com >>> "Mahoney,Janice A" 1/19/2010 9:15 AM >>> My understanding is that it is the job, not the employee that must be monitored. So if you have three people using the same process of changing the stainer, only one of those people needs to be tested. Please correct me if I am wrong. Thanks, Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Monday, January 18, 2010 4:19 PM To: sjkitten@live.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP standard for formalin and xyelene testing CAP standard says formaldehyde testing should be done for each employee as an eight hour exposure and a short term exposure limit. If two tests done at least one week apart are under action levels, there is no need for further ongoing monitoring unless new process, employee, or other factor that might increase exposure is introduced. Or if an employee feels they have a problem and requests it. So I guess each employee is tested twice and then that's it unless you over the limit. Xylene is similar- once you have two tests under action levels, there is no need for ongoing monitoring unless things change as above. Jeffrey Silverman HT HTL QIHC (ASCP) Pathologists' Assistant- Lab Safety Officer Southside Hospital NSLIJHS Bay Shore, NY USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From thecitan <@t> yahoo.com Tue Jan 19 09:32:59 2010 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Tue Jan 19 09:33:02 2010 Subject: [Histonet] Need serviceman for tbs processor in southern California. Message-ID: <1682942973-1263915177-cardhu_decombobulator_blackberry.rim.net-1315351854-@bda950.bisx.prod.on.blackberry> Anyone have contact info for a local serviceman that can do PM and regular maintenance on a TBS ATP1? Sent from my Verizon Wireless BlackBerry From alisha <@t> ka-recruiting.com Tue Jan 19 10:25:43 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Jan 19 10:25:06 2010 Subject: [Histonet] Las Vegas Histology Supervisor Job Message-ID: <420710856.1263918343463.JavaMail.cfservice@webserver57> Dear Histonet Subscribers, Would you consider relocating if the employer were willing to pay for your relocation? Are you interested in learning about other job opportunities for free? Are you interested in learning what other laboratory professionals are making in different areas of the country? Are you looking for better hours, or higher pay, or to be given the opportunity to work your way up the career ladder? If you answered yes to any of these questions, please contact me to learn about other career opportunities. I am the founder of K.A. Recruiting, a healthcare recruiting company that specializes in your industry. I have clients across the United States that hire me to find them talented lab professionals, like yourself. My service is completely FREE to the job seeker. Not only is my service free, I will also be able to tell you about "hidden jobs" that may never be posted on websites and I will be able to guide you every step of the way through the interview process (helping you to secure the job of your dreams and assist with the salary negotiation process, ensuring that you will be paid the highest rate possible for your experience level). Please take a moment to read through the highlighted opportunity below, as well as the other job opportunities I am working on currently on. Highlighted Job Opportunity! One particular client I am working with is looking for a Histology Supervisor for a lab in Las Vegas, NV. This lab is looking for someone with experience, HT(ASCP) or HTL(ASCP) certified, and someone who either lives in or is willing to relocate to Las Vegas, NV. Las Vegas, Nevada is perhaps one of the most exciting places in the entire country. While most people consider the casino portal to be more of a tourism capital than a long term residency, there are plenty of people who have found an excellent home in this city. Sunshine days usually occur 300 days out of the year, and there are very little rainy days. My client is offering an excellent compensation package, relocation assistance, and full benefits. Below is a list of some of the other great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Opportunities: Histotechs/Cytotechs CT - Histology Operations Manager Southern CA - Histology Supervisor New York City - Surgical Pathology and Histology Supervisor New York City - Histotech 3rd shift Las Vegas, NV - Histotech 3rd shift GA - Histotech 1st shift OK - Histotech 1st shift (with opportunity to be promoted to supervisor) Long Island, NY - Cytotech PA - Cytology Supervisor CT - Cytotech Palm Springs, CA - Histotech - 1st shift If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Happy 2010! Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com * Please note change in address and phone number From greenjumpyone <@t> hotmail.com Tue Jan 19 10:57:00 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Tue Jan 19 10:57:07 2010 Subject: [Histonet] Downtime Procedure In-Reply-To: References: Message-ID: Does anyone have specific downtime procedures for when the Anatomic Pathology area computer goes down? The AP area in my facility does not have a downtime procedure and we ran into an issue yesterday when the computer system went down. We processed, on the fly, as it were, but I now recognize our deficiency in not having a formal procedure. Can anyone provide any examples for me to work off of, so that I don't have to recreate the wheel? Thanks for any help! _________________________________________________________________ Hotmail: Trusted email with Microsoft?s powerful SPAM protection. http://clk.atdmt.com/GBL/go/196390706/direct/01/ From trathborne <@t> somerset-healthcare.com Tue Jan 19 11:04:41 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jan 19 11:04:47 2010 Subject: [Histonet] MGMT Message-ID: Thank you all, for your suggestions for who does this test. Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From janderson <@t> halozyme.com Tue Jan 19 11:31:19 2010 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Tue Jan 19 11:32:13 2010 Subject: [Histonet] Thank You! and text search Message-ID: <5F7CC9B788911848A79BC83453A3D6530360FD826D@Tomlinson.hti.com> Dear Histonetters, Thank you so so much for replying to my request for information regarding HT qualifications. I look forward studying, learning, and taking the exam (and putting those 2 or 3 letters after my name). Does anyone know where I can buy a copy of the Lee Luna text? Thanks again for your continued help! All the best, Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. From Timothy.Morken <@t> ucsfmedctr.org Tue Jan 19 11:46:30 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Tue Jan 19 11:46:38 2010 Subject: [Histonet] RE: Thank You! and text search In-Reply-To: <5F7CC9B788911848A79BC83453A3D6530360FD826D@Tomlinson.hti.com> References: <5F7CC9B788911848A79BC83453A3D6530360FD826D@Tomlinson.hti.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C01219EBC47@EXMBMCB15.ucsfmedicalcenter.org> Good luck! But I suggest not getting the Lee Luna text. Unless they have a new edition with corrections, the original edition, which I have, does not have a table of contents and has extensive page number errors in the index making much of the information unavailable. Luna also had an unfortunate habit of putting information in random "thoughts" that do not make it easy to find any particular bit of information you may want. All in all one of the most disappointing books I have ever bought considering his contributions to Histotechnology. The books by Carson and Bancroft will serve you much better. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Tuesday, January 19, 2010 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thank You! and text search Dear Histonetters, Thank you so so much for replying to my request for information regarding HT qualifications. I look forward studying, learning, and taking the exam (and putting those 2 or 3 letters after my name). Does anyone know where I can buy a copy of the Lee Luna text? Thanks again for your continued help! All the best, Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From greenjumpyone <@t> hotmail.com Tue Jan 19 11:58:12 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Tue Jan 19 11:58:16 2010 Subject: [Histonet] RE: Eosin and Leica XL stainer In-Reply-To: References: Message-ID: Cheryl, Yes, the Lecia XL is supposed to vibrate when it lifts the racks. If it's not doing that, you need to call and ask for some assistance. It sounds to me like there is something either turned off or broken in the setup. Regarding the eosin getting the alcohol pink, that happens to us all the time. We go from eosin to 95%, 3-100% and then 3-xylenes before coverslipping. It doesn't seem to affect the quality of our staining. hopper _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/196390709/direct/01/ From tkngflght <@t> yahoo.com Tue Jan 19 12:00:38 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Jan 19 12:00:42 2010 Subject: [Histonet] PM and repair vendors - need contact info Message-ID: <261116.34054.qm@web50903.mail.re2.yahoo.com> Hi All! ? Again I need help from you sages of histology. ? I offered to find a selection of repair and PM vendors for a laboratory.? National reach is preferred, but if you have a stellar local group -- please share?? I'd like to know of your experience with them and how they came to be your favorite group. ? We've had a few very negative experiences lately and I figured I didn't have to?suffer through a bad repair when I have you guys! ? Thanks for your generosity! ? Cheryl Kerry, HT(ASCP) ? ? From rjbuesa <@t> yahoo.com Tue Jan 19 15:37:35 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 19 15:37:39 2010 Subject: [Histonet] CAP standard for formalin and xyelene testing In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A55C9@EXCHMBC2.ad.ah.local> Message-ID: <232857.24614.qm@web65710.mail.ac4.yahoo.com> Not all histotechs work in the same way, so I always monitored the job (as Janice points out) but also the staff member. I remember having different results for staff for the same task. We used to monitor the whole staff (21) during a whole year, meaning that each month there was somebody being tested. We monitores for formalin and xylene, until we stop using xylene. We also had an outside company to monitor the whole lab during one single day. Ren? J. --- On Tue, 1/19/10, Mahoney,Janice A wrote: From: Mahoney,Janice A Subject: RE: [Histonet] CAP standard for formalin and xyelene testing To: "'Jeffrey Silverman'" , "sjkitten@live.com" Cc: "histonet@lists.utsouthwestern.edu" Date: Tuesday, January 19, 2010, 9:15 AM My understanding is that it is the job, not the employee that must be monitored.? So if you have three people using the same process of changing the stainer, only one of those people needs to be tested. Please correct me if I am wrong. Thanks, Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Monday, January 18, 2010 4:19 PM To: sjkitten@live.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP standard for formalin and xyelene testing CAP standard says formaldehyde testing should be done for each employee as an eight hour exposure and a short term exposure limit. If two tests done at least one week apart are under action levels, there is no need for further ongoing monitoring unless new process, employee, or other factor that might increase exposure is introduced. Or if an employee feels they have a problem and requests it. So I guess each employee is tested twice and then that's it unless you over the limit. Xylene is similar- once you have two tests under action levels, there is no need for ongoing monitoring unless things change as above. Jeffrey Silverman HT HTL QIHC (ASCP) Pathologists' Assistant- Lab Safety Officer Southside Hospital NSLIJHS Bay Shore, NY USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees.? Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.? If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.? Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening.? Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.? Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Tue Jan 19 15:58:28 2010 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Jan 19 15:58:41 2010 Subject: [Histonet] CAP standard for formalin and xyelene testing In-Reply-To: <232857.24614.qm@web65710.mail.ac4.yahoo.com> Message-ID: We also monitored by task, and by employee with badges. But, even though we've never been over threshold limits, we have an outside company come in once per year. Its a precautionary step lest the least procedural change slip by us. This also acts as an insurance for those that might seek to find fault with our monitoring. As we all know, you can smell xylene long before it becomes dangerous. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Tuesday, January 19, 2010 16:38 To: 'Jeffrey Silverman'; sjkitten@live.com; Janice AMahoney Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP standard for formalin and xyelene testing Not all histotechs work in the same way, so I always monitored the job (as Janice points out) but also the staff member. I remember having different results for staff for the same task. We used to monitor the whole staff (21) during a whole year, meaning that each month there was somebody being tested. We monitores for formalin and xylene, until we stop using xylene. We also had an outside company to monitor the whole lab during one single day. Ren? J. --- On Tue, 1/19/10, Mahoney,Janice A wrote: From: Mahoney,Janice A Subject: RE: [Histonet] CAP standard for formalin and xyelene testing To: "'Jeffrey Silverman'" , "sjkitten@live.com" Cc: "histonet@lists.utsouthwestern.edu" Date: Tuesday, January 19, 2010, 9:15 AM My understanding is that it is the job, not the employee that must be monitored.? So if you have three people using the same process of changing the stainer, only one of those people needs to be tested. Please correct me if I am wrong. Thanks, Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Monday, January 18, 2010 4:19 PM To: sjkitten@live.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP standard for formalin and xyelene testing CAP standard says formaldehyde testing should be done for each employee as an eight hour exposure and a short term exposure limit. If two tests done at least one week apart are under action levels, there is no need for further ongoing monitoring unless new process, employee, or other factor that might increase exposure is introduced. Or if an employee feels they have a problem and requests it. So I guess each employee is tested twice and then that's it unless you over the limit. Xylene is similar- once you have two tests under action levels, there is no need for ongoing monitoring unless things change as above. Jeffrey Silverman HT HTL QIHC (ASCP) Pathologists' Assistant- Lab Safety Officer Southside Hospital NSLIJHS Bay Shore, NY USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees.? Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.? If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.? Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening.? Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.? Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From mariawoodruff <@t> hotmail.com Tue Jan 19 17:27:45 2010 From: mariawoodruff <@t> hotmail.com (maria woodruff) Date: Tue Jan 19 17:27:49 2010 Subject: [Histonet] First time user question - Marmed Saw question Message-ID: I'm a first time user of this site but wondered if anyone could please help. I need to purchase a Marmed Bone Saw for our labs here in Australia and am having NO luck whatsoever getting a reply via email from Marmed. I used their saw in Singapore and found it excellent .... but wondered if anyone has any other recommendations as I am frustrated by their lack of customer service. I will be cutting bones up to a max of 3 inches wide and have a budget up to $1000. Many thanks Mia _________________________________________________________________ Send us your Hotmail stories and be featured in our newsletter http://clk.atdmt.com/UKM/go/195013117/direct/01/ From kc <@t> ka-recruiting.com Tue Jan 19 18:07:25 2010 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Tue Jan 19 18:07:33 2010 Subject: [Histonet] Updated Histology Jobs! Message-ID: <1377611543.1263946045122.JavaMail.cfservice@webserver53> Dear Histonet Subscribers, I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. Below is a list of some of the other Histology opportunities we are currently working on. Histotechs/Cytotechs: ****Las Vegas, NV - Histology Supervisor **** New Opening!!! Connecticut- Histology Operations Manager Southern CA - Histology Supervisor New York City - Surgical Pathology and Histology Supervisor New York City - Histotech 3rd shift Las Vegas, NV - Histotech 3rd shift Central Georgia - Histotech 1st shift Oklahoma - Histotech 1st shift (with opportunity to be promoted to supervisor) Long Island, NY - Cytotech New York City - Cytotech Pennsylvania - Cytology Supervisor Connecticut ? Cytotech If you're interested in learning more about any of these opportunities then please email me a resume and let me know how best to get in touch with you. If none of these are a fit please let me know what you'd be interested in and where you're looking so I can tailor a search for you. With the New Year upon us many of our clients have fresh hiring budgets and will be looking to add people over the next several months. We work on positions at all levels and cover the entire US. To view some additional opportunities please visit our website at www.ka-recruiting.com . Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From anonwums1 <@t> gmail.com Tue Jan 19 20:17:42 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Tue Jan 19 20:17:47 2010 Subject: [Histonet] Lineage tracing in bone Message-ID: <858249121001191817x4a91dceduc597e7a88bed02aa@mail.gmail.com> Hi all, As my thesis project moves forward, my commitee has asked me to do some lineage tracing in mouse bone. For those of you not familiar with this, you mate a mouse expressing a Cre recombinase to another mouse expressing a reporter gene with a premature stop codon that can be floxed out. The reporter is usually lacZ or GFP. The Cre will remove the stop codon and allow expression of your reporter in the cells the cre is expressed in (and all its progeny). Here's the problem: I don't have access to a tape transfer system (yet) so I have to use fixed decalcified bones. I would prefer to use paraffin embedded sections due to the improved morphology. To make things more complicated. I probably will even have to do colocalization with other antibodies. So here are the options that I am familiar with for reporter strains: 1) lacZ reporters (http://jaxmice.jax.org/strain/003474.html) I worry that the fixation or embedding will destroy the lacZ. It's pretty common in developmental biology to see people use these for unfixed tissue to directly assay lacZ. There was some discussion in the archives ( http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-February/035626.html) on a method to embed in paraffin and preserve lacZ but it seems a bit complicated. Has anyone successfully used an anti-lacZ antibody to do this in bone? 2) eGFP reporters (http://jaxmice.jax.org/strain/004077.html) Jackson's website notes that this isn't suitable for immunohistochemistry because expression is too low. Since bone is so autofluorescent, I really doubt I'd ever see it. I do have a chicken anti-GFP antibody, which seems to work really great in paraffin sections with some mouse strains (col2.3-GFP) but doesn't seem to work with others (CX3CR1-GFP) when I come in with a Dylight488 anti-chicken. This seems to occur even though they seem to have similar brightness when assayed by FACS... I'm not sure why. Has anyone used an anti-GFP antibody for lineage tracing in bone or other fixed tissues? If you're aware of any other strains or methods to accomplish this, I would greatly appreciate your suggestions. There is a small chance that I may get access to a Cryojane at some point in the future, and I would also welcome comments on how feasible this would be using that system. Thanks, Adam From pruegg <@t> ihctech.net Tue Jan 19 20:36:42 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jan 19 20:37:26 2010 Subject: [Histonet] RE: Thank You! and text search In-Reply-To: <1AAF670737F193429070841C6B2ADD4C01219EBC47@EXMBMCB15.ucsfmedicalcenter.org> References: <5F7CC9B788911848A79BC83453A3D6530360FD826D@Tomlinson.hti.com> <1AAF670737F193429070841C6B2ADD4C01219EBC47@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: I agree Tim, it is impossible to just quickly look something up in Lee Luna's book because there is no indexing, it is a shame because he has some great pictures but if you can't use it then it doesn't do you much good. Freida Carson's new edition is excellent. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Tuesday, January 19, 2010 10:47 AM To: Jennifer Anderson; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Thank You! and text search Good luck! But I suggest not getting the Lee Luna text. Unless they have a new edition with corrections, the original edition, which I have, does not have a table of contents and has extensive page number errors in the index making much of the information unavailable. Luna also had an unfortunate habit of putting information in random "thoughts" that do not make it easy to find any particular bit of information you may want. All in all one of the most disappointing books I have ever bought considering his contributions to Histotechnology. The books by Carson and Bancroft will serve you much better. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Tuesday, January 19, 2010 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thank You! and text search Dear Histonetters, Thank you so so much for replying to my request for information regarding HT qualifications. I look forward studying, learning, and taking the exam (and putting those 2 or 3 letters after my name). Does anyone know where I can buy a copy of the Lee Luna text? Thanks again for your continued help! All the best, Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfitz <@t> 007group.com Tue Jan 19 21:31:37 2010 From: cfitz <@t> 007group.com (Cathy) Date: Tue Jan 19 21:31:46 2010 Subject: [Histonet] Downtime Procedure In-Reply-To: References: Message-ID: <0EFE1E0C6B584343936B794DDBED0F94@your8ba846406f> It would be good to hear any responses to this question. Last October our entire facility's computer system was down for 2 1/2 days. We do have downtime log sheets that we implemented but no formal procedure. Management is now looking at how the crash was handled and for ways to improve things. The downtime log sheets were very useful but I'm sure there is much more that we could be doing. Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Tuesday, January 19, 2010 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Downtime Procedure Does anyone have specific downtime procedures for when the Anatomic Pathology area computer goes down? The AP area in my facility does not have a downtime procedure and we ran into an issue yesterday when the computer system went down. We processed, on the fly, as it were, but I now recognize our deficiency in not having a formal procedure. Can anyone provide any examples for me to work off of, so that I don't have to recreate the wheel? Thanks for any help! _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/196390706/direct/01/____________________________ ___________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Wed Jan 20 07:34:29 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Jan 20 07:36:56 2010 Subject: [Histonet] cassettes for microwave tissue processing Message-ID: <24A4826E8EF0964D86BC5317306F58A542559DE5F4@mmc-mail.ad.mhsil.com> We are expecting our new microwave tissue processor in less than a month. Currently we are using biopsy pads for a lot of our small specimens for regular processing. I know the use of biopsy pads is highly discouraged on the microwave tissue processors, and vendors have suggested using biopsy bags for our small biopsies when they need microwave processing. I am also considering some biopsy cassettes and would like some feedback on other's experiences. Obviously there are many types of biopsy cassettes on the market, some with compartments, some with micromesh, some with just small holes. I would like some feedback on what has worked best for microwave users. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Sharon.Davis-Devine <@t> carle.com Wed Jan 20 07:49:09 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Jan 20 07:49:53 2010 Subject: [Histonet] Slide writer Message-ID: <50003EC02E2CEA4583BEB3CD08EAC1E090DD87@EXCHANGEBE2.carle.com> Hello Histonetters! I know this has been discussed before but at the time we did not need this instrument so I didn't pay much attention to the posts. So here is my request. We need a new slide writer. We currently have a Thermo Electron Microwriter which must be a lemon because we have had nothing but problems with it. We want to replace this instrument with one that we can count on. So please all you labs out there that have a slide writer let me know what kind of slide writer you use and how it has performed for you. Thanks so much. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From KPercival <@t> wyeth.com Wed Jan 20 07:54:52 2010 From: KPercival <@t> wyeth.com (Percival Karen) Date: Wed Jan 20 07:55:10 2010 Subject: [Histonet] Downtime Procedure In-Reply-To: <0EFE1E0C6B584343936B794DDBED0F94@your8ba846406f> References: <0EFE1E0C6B584343936B794DDBED0F94@your8ba846406f> Message-ID: <4B56C4DC02000011001F7CBB@gv01a67m.gv.us.pri.wyeth.com> At my former place of employment, I had created a downtime procedure to manage computer downtime for my staff which consisted of histotechs, cytotechs, admin staff, and a PA (grossing). I currently do not have a copy of that procedure, but I will try to get it a copy of it from a former colleague who may still have access to it. Having a computer downtime procedure in place worked out really well. Everyone knew what to do, and panic and confusion were minimized, and getting the information back into the computer when the system came back up was organized and quick. We were able to continue with specimen processing, and the pathologists even received their slides on time, for the most part. It actually took quite a while to create the procedure but was well worth the time spent. Mostly it was simply spelling out detail by detail how to manually perform the tasks that would keep processess moving along as much as possible, especially those that did not need a computer; such as, accessioning specimens with "paper numbers", processing fluids and paps, gross dictation, I think, was done on an tape recorder, etc. Anyway, I'll try to get a copy of the procedure. Sorry I don't currently have it. Karen Percival, BS, HT Research Scientist II Pfizer Research DSRD 1 Burtt Road G3025 Andover, MA 01810 888-577-1500 x 4058 kpercival @wyeth.com >>> "Cathy" 1/19/2010 10:31 PM >>> It would be good to hear any responses to this question. Last October our entire facility's computer system was down for 2 1/2 days. We do have downtime log sheets that we implemented but no formal procedure. Management is now looking at how the crash was handled and for ways to improve things. The downtime log sheets were very useful but I'm sure there is much more that we could be doing. Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Tuesday, January 19, 2010 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Downtime Procedure Does anyone have specific downtime procedures for when the Anatomic Pathology area computer goes down? The AP area in my facility does not have a downtime procedure and we ran into an issue yesterday when the computer system went down. We processed, on the fly, as it were, but I now recognize our deficiency in not having a formal procedure. Can anyone provide any examples for me to work off of, so that I don't have to recreate the wheel? Thanks for any help! _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/196390706/direct/01/____________________________ ___________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Wed Jan 20 09:02:56 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Jan 20 09:03:44 2010 Subject: [Histonet] HT/HTL vacancy Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B645FC3@chi2k3ms01.columbuschildrens.net> We have a vacancy for an HT/HTL in our Anatomic Pathology department. The ideal candidate will have the opportunity to perform all duties in pathology, including but not limited to tissue processing (both traditional and microwave) routine microtomy, cryotomy, H&E staining, special stains, IHC, IF, ISH and EM. There is also the opportunity to get involved in test development and research. No regular weekend or holiday work (on-call). Relocation assistance available Team player a MUST. For more information please contact me, or apply on-line at https://www.healthcaresource.com/columbus/index.cfm?fuseaction=search.jo bDetails&template=dsp_job_details.cfm&cJobId=878955 Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From rfields <@t> gidocs.net Wed Jan 20 09:08:36 2010 From: rfields <@t> gidocs.net (Rosa Fields) Date: Wed Jan 20 09:10:00 2010 Subject: [Histonet] cassettes for microwave tissue processing References: <24A4826E8EF0964D86BC5317306F58A542559DE5F4@mmc-mail.ad.mhsil.com> Message-ID: <07732CE52EC3174AB891DE1C62DB4D8FC7B67F@GIEXCHANGE.gidocs.net> I would stay away from the micromesh/screen cassettes, they can trap air bubbles. We microwave process all of our specimens in the McCormick Microflow 2 cassettes; (I guess now Lecia/Surgipath).. we get them from either Stat Lab or VWR. These cassettes are wonderful, they don't trap any air so they don't float! http://www.mccormickscientific.com/microflow.asp?PCID=838 Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, January 20, 2010 7:34 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cassettes for microwave tissue processing We are expecting our new microwave tissue processor in less than a month. Currently we are using biopsy pads for a lot of our small specimens for regular processing. I know the use of biopsy pads is highly discouraged on the microwave tissue processors, and vendors have suggested using biopsy bags for our small biopsies when they need microwave processing. I am also considering some biopsy cassettes and would like some feedback on other's experiences. Obviously there are many types of biopsy cassettes on the market, some with compartments, some with micromesh, some with just small holes. I would like some feedback on what has worked best for microwave users. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jan 20 09:13:04 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Jan 20 09:13:15 2010 Subject: [Histonet] Job opening Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C56FA3990E@HPEMX3.HealthPartners.int> Regions Hospital is a full-service, private hospital and a Level I Adult and Pediatric trauma center. Employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and "Best Care, Best Experience", has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. Regions Hospital Histology Department is currently seeking professional, self-motivated, quality and team-focused Histotechnicians to join our team. As our ideal candidate, you are HT ASCP certified or eligible and have 1 year of hospital laboratory experience as a Histotechnician. As a Histotechnician at Regions Hospital, you will join an innovative and collaborative group of professionals in a supportive work environment. You will work in a department that has 13 Pathologists, uses barcoding technology, performs special and immunohistochemistry staining, and is embarking on a laboratory expansion. You will support Regions Hospital and HealthPartners network of clinics processing all types of surgical pathology specimens. As part of our team, you will share in the responsibility for quality assurance and participate in continuing education projects for the department. The Twin Cities is the fastest growing urban center in the Midwest. Living here you'll enjoy one of the nation's most economically robust regions, situated among scenic forests, rivers, lakes, parks, and golf courses. The Twin Cities region is home of two thriving downtowns, internationally acclaimed orchestras and museums, and five major league sports teams. For consideration, please apply at www.regionshospital.com under career opportunities Job ID #'s: 16358, 16360, 16376 -EOE Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From laurie.colbert <@t> huntingtonhospital.com Wed Jan 20 09:19:56 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Jan 20 09:20:02 2010 Subject: [Histonet] Slide writer Message-ID: <57BE698966D5C54EAE8612E8941D768307CD8906@EXCHANGE3.huntingtonhospital.com> Hi Sharon, We have 5 Slidemates from Fisher. These units were previously manufactured/marketed by Accuplace and were called PSLIM's. We have a unit set up at each microtome. Our cassettes are barcoded, and we simply scan the barcode on the cassette, tell the unit how many slides we want, and our slide is ready before the section is cut and laid out on the waterbath. This method allows positive identification, because you are printing the slide at the time you are cutting the block - no batch printing. We have had issues with the quality of the print on the slides and some jamming issues, but Accuplace and Fisher have addressed these issues. I love the units despites these occasional setbacks and highly recommend them. Laurie Colbert Huntington Hospital Pasadena, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, January 20, 2010 5:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide writer Hello Histonetters! I know this has been discussed before but at the time we did not need this instrument so I didn't pay much attention to the posts. So here is my request. We need a new slide writer. We currently have a Thermo Electron Microwriter which must be a lemon because we have had nothing but problems with it. We want to replace this instrument with one that we can count on. So please all you labs out there that have a slide writer let me know what kind of slide writer you use and how it has performed for you. Thanks so much. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Wed Jan 20 09:37:10 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Jan 20 09:36:45 2010 Subject: [Histonet] Histotech Job Opportunity in Virginia Message-ID: <1348838174.1264001830002.JavaMail.cfservice@webserver54> Dear Histonet Subscribers, Would you consider relocating if the employer were willing to pay for your relocation? Are you interested in learning about other job opportunities for free? Are you interested in learning what other laboratory professionals are making in different areas of the country? Are you looking for better hours, or higher pay, or to be given the opportunity to work your way up the career ladder? If you answered yes to any of these questions, please contact me to learn about other career opportunities. I am the founder of K.A. Recruiting, a healthcare recruiting company that specializes in your industry. I have clients across the United States that hire me to find them talented lab professionals, like yourself. My service is completely FREE to the job seeker. Not only is my service free, I will also be able to tell you about "hidden jobs" that may never be posted on websites and I will be able to guide you every step of the way through the interview process (helping you to secure the job of your dreams and assist with the salary negotiation process, ensuring that you will be paid the highest rate possible for your experience level). Please take a moment to read through the highlighted opportunity below, as well as the other job opportunities I am working on currently on. Highlighted Job Opportunity! One particular client I am working with is one of the top hospital systems in the country. This hospital in Northern Virginia is looking for an evening shift histotech. It's a 450 bed non-profit hospital, that is newly renovated and had very modern facilities. Its south of DC and North of Richmond, VA. The school system is outstanding - standardized test scores are far above the national average. This position has a very competitive base salary, great benefits, and an unparalled retirement package. Below is a list of some of the other great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Opportunities: Histotechs/Cytotechs CT - Histology Operations Manager Southern CA - Histology Supervisor New York City - Surgical Pathology and Histology Supervisor New York City - Histotech 3rd shift Las Vegas, NV - Histotech 3rd shift Las Vegas, NV - Histology Supervisor GA - Histotech 1st shift OK - Histotech 1st shift (with opportunity to be promoted to supervisor) Long Island, NY - Cytotech PA - Cytology Supervisor CT - Cytotech Palm Springs, CA - Histotech - 1st shift If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Happy 2010! Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com * Please note change in address and phone number From relia1 <@t> earthlink.net Wed Jan 20 10:06:58 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jan 20 10:07:06 2010 Subject: [Histonet] RELIA Solutions Histology Careers Bulletin 01/20/2010 Message-ID: Hi Histonetters!! Happy New Year! What?s your New Year?s Resolution? Of course I am going to try hard to eat right and exercise. But My Resolution is to help even more histo techs find the right opportunity in the right place at the right time. In 2009 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get better salaries, shifts and benefits in one of the most challenging economic climates in decades!. In 2010 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately their quality of life. Whatever you want to do Wherever you want to be. Let Me Help! I offer over 25 years of experience as a recruiter and over 7 years of experience working exclusively in the nationwide permanent placement of histology professionals. Take Advantage of RELIA?s Career Coaching Program. Free of Charge RELIA offers: Assistance with updating creating or critiquing your resume Tips on interviewing Encouragement and Assistance during the course of your job search Responsiveness ? I will respond to your e-mails/phone calls within 24 hours or less. Troubleshooting ? if your job search is stalled or you can?t get in the company you are interested in. Personalized Job Search ? customized to your experience, wants and needs. Complete Confidentiality - Your career and my coaching is a relationship of trust. I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. Here are some of my current openings: HISTOLOGY/PATHOLOGY MANAGEMENT FL ? Palm Beaches Histology Supervisor/Lead Tech NY- Orange/Rockland County Histology Supervisor MA ? Cape Cod Pathology Supervisor OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA ? Central CA ? Pathology Supervisor CA ? Los Angeles ?Histology Supervisor HISTOTECHS FL-Orlando Electron Microscopy Specialist FL- Palm Beaches-Lead Histotech private Lab FL ? Pensacola- Histotech private lab GA - Immunohistochemistry Tech-private lab 2nd shift MD ? Histotech - Hospital MA ? Boston Histotech day and evening shifts MA ? Boston ? Immunohistochemistry Specialist TX ? Austin ?Night Shift Histotech TX ? Fort Worth MOHS Tech part time NY-Orange/Rockland County-Brand New Lab NYS license req PATHOLOGY/PATHOLOGIST ASSISTANT NC ? Charlotte PA grad from NAACLES program required MA ? Boston Hospital environment If you or any of your friends would like more information on RELIA?S Career Coaching, about any of the positions listed or would like to talk about a future job search please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember it never hurts to look Hope to hear from you soon. Thanks-Pam Thank You! Pam M. Barker President RELIA 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia From greenjumpyone <@t> hotmail.com Wed Jan 20 10:14:46 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Wed Jan 20 10:14:51 2010 Subject: [Histonet] flammable liquid storage In-Reply-To: References: Message-ID: I was told that we are allowed to have 2 gallons of flammable liquid contained within a flammable cabinet per 100 sq. ft. My question is the following: Is that square footage for the *entire* clinical laboratory or *only* the histology area of the lab? My inclination is to say the entire clinical lab, not just histo, but I need to be certain. I am referring to the AHCA NFPA 99 item 11.7.2 for the state of Florida. The following is the exact wording: 11.7.2.3.2 The total volume of CLass I, II, and IIIA liquids, including those contained in approved storage cabinets and safety cans, shall not exceed 7.57 L (2 gal) per 9.29 m2 (100ft2) (sorry, I couldn't do a superscript!) Can anyone offer any help on this? Thanks! hopper _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/196390710/direct/01/ From SGoodacre <@t> chw.org Wed Jan 20 10:19:57 2010 From: SGoodacre <@t> chw.org (Goodacre, Suzanne) Date: Wed Jan 20 10:20:03 2010 Subject: [Histonet] EBER ISH problems Message-ID: Hello, Our Histo lab currently uses the Leica Microsystems Bond instrument for EBER ISH. We noticed that eosinophils readily pick up the DAB detection reagents that we use. I've attempted to alter the EBER ISH protocol on the BOND so that the eosinophils don't take on the detection reagents but I'm not having much luck. Does anyone have any tips on how to prevent or avoid the problem? Suzanne Goodacre Research Technologist Micro Molecular Lab Children's Hospital of Wisconsin 414-266-2709 From rjbuesa <@t> yahoo.com Wed Jan 20 10:25:40 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 20 10:25:44 2010 Subject: [Histonet] flammable liquid storage In-Reply-To: Message-ID: <159238.23875.qm@web65701.mail.ac4.yahoo.com> The regulation is 1 gallon per every 24 square feet of laboratory. Ren? J. --- On Wed, 1/20/10, Green JumpyOne wrote: From: Green JumpyOne Subject: [Histonet] flammable liquid storage To: histonet@lists.utsouthwestern.edu Date: Wednesday, January 20, 2010, 11:14 AM I was told that we are allowed to have 2 gallons of flammable liquid contained within a flammable cabinet per 100 sq. ft.? My question is the following:? Is that square footage for the *entire* clinical laboratory or *only* the histology area of the lab?? My inclination is to say the entire clinical lab, not just histo, but I need to be certain. I am referring to the AHCA NFPA 99 item 11.7.2 for the state of Florida.? The following is the exact wording: 11.7.2.3.2? The total volume of CLass I, II, and IIIA liquids, including those contained in approved storage cabinets and safety cans, shall not exceed 7.57 L (2 gal) per 9.29 m2 (100ft2) (sorry, I couldn't do a superscript!) Can anyone offer any help on this? Thanks! hopper ??? ???????? ?????? ??? ? _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/196390710/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Wed Jan 20 10:49:01 2010 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Wed Jan 20 10:49:42 2010 Subject: [Histonet] Diamond Blade Band Saw (s) Message-ID: <002601ca99f0$7820b290$c5d76880@vetmed.wisc.edu> Marmed Inc. has a good website. They custom build the saws, however I do not know how far they distribute. I have seen this small saw or one very similar distributed by others ( sometimes with a name change). Feel free to contact me if you need more information. Vicki From JCBRITTON <@t> Cheshire-Med.COM Wed Jan 20 11:15:27 2010 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Wed Jan 20 11:15:33 2010 Subject: [Histonet] EBER ISH problems In-Reply-To: References: Message-ID: Try switching your DAB kit to Refined Red, this helped us. We also run a negative RNA and positive RNA on the patient also. Josie Britton HT Cheshire Medical Center Keene, NH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goodacre, Suzanne Sent: Wednesday, January 20, 2010 11:20 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] EBER ISH problems Hello, Our Histo lab currently uses the Leica Microsystems Bond instrument for EBER ISH. We noticed that eosinophils readily pick up the DAB detection reagents that we use. I've attempted to alter the EBER ISH protocol on the BOND so that the eosinophils don't take on the detection reagents but I'm not having much luck. Does anyone have any tips on how to prevent or avoid the problem? Suzanne Goodacre Research Technologist Micro Molecular Lab Children's Hospital of Wisconsin 414-266-2709 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From MDiCarlo <@t> KaleidaHealth.Org Wed Jan 20 11:47:18 2010 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Wed Jan 20 11:47:25 2010 Subject: [Histonet] bone-freezing versus formalin fixing Message-ID: <1B73766A27A1554CB2729B6432E8130101D4F2D3@KALEXMB04.KaleidaHealth.org> Hello, I work for two orthopedic surgeons and one would like his whole bone frozen at -80 degrees Celsius and the other one would like it fixed in 10% buffered formalin before slicing on a band saw. I have to photograph and x-ray upon receiving them which isn't always the same day of surgery. The bone which is frozen is better for picture taking but on occasion when I formalin fix a bone, my one boss says there is much better nuclear detail (no muddy appearance of nuclei) and no freezing artifact. I use 10% formic acid for decaling and x-ray for end point decalcification. The formalin penetrates the whole piece of bone slowly from the outside working inward and the inside is still unfixed by the time we slice it which I think would cause the cells to autolyze. Can anyone recommend which is the better way to go so I can make the best H&E slides possible? I appreciate your advice. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From pkarlisch <@t> hmc.psu.edu Wed Jan 20 11:52:08 2010 From: pkarlisch <@t> hmc.psu.edu (Patricia Karlisch) Date: Wed Jan 20 11:52:44 2010 Subject: [Histonet] RE: Billing for Research Only Antibodies Message-ID: <4B56FC78.07B7.008C.1@hmc.psu.edu> Hi Histonetters: How do you handle antibodies that are not yet IVD and are still RUO. I understand the disclaimer on the reports but does that make the test billable? Do any of you charge for the IHC stain clinically. We have 3 IHC stains that are RUO, one of which is SV40T. We are not billing for this test, but we do more than 200/year. Thanks in advance, Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From kc <@t> ka-recruiting.com Wed Jan 20 12:12:02 2010 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Wed Jan 20 12:10:50 2010 Subject: [Histonet] Updated Histology Jobs! Message-ID: <392637612.1264011122488.JavaMail.cfservice@webserver54> Good afternoon Histoneters, I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. Below is a list of some of the other Histology opportunities we are currently working on. Histotechs/Cytotechs: ****Northern, VA - Histotechnologist 2nd shift **** New Opening!!! ****Las Vegas, NV - Histology Supervisor **** New Opening!!! Connecticut- Histology Operations Manager Southern CA - Histology Supervisor New York City - Surgical Pathology and Histology Supervisor New York City - Histotech 3rd shift Las Vegas, NV - Histotech 3rd shift Central Georgia - Histotech 1st shift Oklahoma - Histotech 1st shift (with opportunity to be promoted to supervisor) Long Island, NY - Cytotech New York City - Cytotech Pennsylvania - Cytology Supervisor Connecticut ? Cytotech If you're interested in learning more about any of these opportunities then please email me a resume and let me know how best to get in touch with you. If none of these are a fit please let me know what you'd be interested in and where you're looking so I can tailor a search for you. With the New Year upon us many of our clients have fresh hiring budgets and will be looking to add people over the next several months. We work on positions at all levels and cover the entire US. To view some additional opportunities please visit our website at www.ka-recruiting.com . Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From jeanie.meyers <@t> trumbulllabs.com Wed Jan 20 13:17:32 2010 From: jeanie.meyers <@t> trumbulllabs.com (Jeanie Meyers) Date: Wed Jan 20 13:16:04 2010 Subject: [Histonet] (no subject) Message-ID: <201001201916.o0KJG1uZ008060@mail45.atl.registeredsite.com> Does anyone know and be willing to share how much companies are typically willing to pay for histology blocks, slides or cases used exclusively for research purposes? Jeanie Meyers, Laboratory Manager Trumbull Laboratories, LLC 7550 Wolf River Boulevard, Suite 200 Germantown, Tennessee 38138 (901)542-6806 (901)507-2633 Fax CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Herrick.James <@t> mayo.edu Wed Jan 20 17:29:34 2010 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Wed Jan 20 17:29:38 2010 Subject: [Histonet] Question on Tissue Processing Message-ID: <7267A64D75F58241B577876D8A885631015A991E@msgebe41> Hello all, We have recently received a Leica TP1020 tissue processor and will be setting it up very soon. Our specimens will be embedded in MMA. Would anyone have any protocols that have worked well with rat and mouse femurs/tibias, as well as for larger specimens (e.g. goat and sheep spines, goat jaws, etc.)? Any information would be greatly appreciated. Thanks again. Jim Herrick Senior Research Technologist Department of Orthopedics Bone Histomorphometry Core Lab Phone: (507) 255-5946 fax: (507) 266-9451 Email: herrick.james@mayo.edu ______________________ Mayo Clinic 200 First Street SW Rochester, MN 55905 www.mayoclinic.org From amosbrooks <@t> gmail.com Wed Jan 20 18:39:03 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Jan 20 18:39:08 2010 Subject: [Histonet] cassettes for microwave tissue processing Message-ID: <582736991001201639wb8d5907xc59671526529bc1c@mail.gmail.com> Hi James, Biopsy cassettes can be good to hold tiny specimens, but some of them are just terrible. The ones that are like a nylon bag float when you put them in the rack to load the processor until they finally take on enough solution to sink. They have about the same reagent carry over as the biopsy pads because the solutions just don't drain out of them well. And it is really difficult to remove an air bubble during embedding. You actually end up having to over fill the mold to keep any bubbles out. The ones that are just plastic with tiny holes are much better. Amos Message: 9 Date: Wed, 20 Jan 2010 07:34:29 -0600 From: "Vickroy, Jim" Subject: [Histonet] cassettes for microwave tissue processing To: "Histonet@lists.utsouthwestern.edu" Message-ID: <24A4826E8EF0964D86BC5317306F58A542559DE5F4@mmc-mail.ad.mhsil.com> Content-Type: text/plain; charset="us-ascii" We are expecting our new microwave tissue processor in less than a month. Currently we are using biopsy pads for a lot of our small specimens for regular processing. I know the use of biopsy pads is highly discouraged on the microwave tissue processors, and vendors have suggested using biopsy bags for our small biopsies when they need microwave processing. I am also considering some biopsy cassettes and would like some feedback on other's experiences. Obviously there are many types of biopsy cassettes on the market, some with compartments, some with micromesh, some with just small holes. I would like some feedback on what has worked best for microwave users. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 From sheila_adey <@t> hotmail.com Thu Jan 21 04:29:20 2010 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Jan 21 04:29:24 2010 Subject: [Histonet] Looking for the Dako rep for south western Ontario Message-ID: I'm looking for the Dako rep in South western Ontario, Cda. Thanks. Sheila Adey _________________________________________________________________ From pruegg <@t> ihctech.net Thu Jan 21 07:41:06 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Jan 21 07:41:50 2010 Subject: [Histonet] Slide writer In-Reply-To: <50003EC02E2CEA4583BEB3CD08EAC1E090DD87@EXCHANGEBE2.carle.com> References: <50003EC02E2CEA4583BEB3CD08EAC1E090DD87@EXCHANGEBE2.carle.com> Message-ID: <0BA1412CBA284B528A9233134F84A7F5@prueggihctechlt> In our new Derm Path lab we went with Leica for slide labeling and block labeling, we designed our own computer program with bar coding and other identifiers and the labelers plugged right into our system, they are not cheap but we really like them. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, January 20, 2010 6:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide writer Hello Histonetters! I know this has been discussed before but at the time we did not need this instrument so I didn't pay much attention to the posts. So here is my request. We need a new slide writer. We currently have a Thermo Electron Microwriter which must be a lemon because we have had nothing but problems with it. We want to replace this instrument with one that we can count on. So please all you labs out there that have a slide writer let me know what kind of slide writer you use and how it has performed for you. Thanks so much. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtaylor <@t> meriter.com Thu Jan 21 07:56:37 2010 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu Jan 21 07:56:43 2010 Subject: [Histonet] CISH - hybridizer Message-ID: <466B666475DE6547BBB0641E540A4BB506451DCD2D@EXVS1.meriter.com> If you do not have heat on your IHC stainer, what instrument do you use for denaturing and hybridization? Does anyone have experience using the MJ Research PTC -100 with in situ slide block from Pegasus Scentific? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Jan 21 08:09:02 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Jan 21 08:09:24 2010 Subject: [Histonet] RE: CISH - hybridizer In-Reply-To: <466B666475DE6547BBB0641E540A4BB506451DCD2D@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB506451DCD2D@EXVS1.meriter.com> Message-ID: Dako has a Hybridizer that holds 20 slides. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean [jtaylor@meriter.com] Sent: Thursday, January 21, 2010 8:56 AM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] CISH - hybridizer If you do not have heat on your IHC stainer, what instrument do you use for denaturing and hybridization? Does anyone have experience using the MJ Research PTC -100 with in situ slide block from Pegasus Scentific? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From beraldofelipe <@t> yahoo.com.br Thu Jan 21 08:26:35 2010 From: beraldofelipe <@t> yahoo.com.br (Felipe Beraldo) Date: Thu Jan 21 08:26:38 2010 Subject: [Histonet] anti rat mast cell protease Message-ID: <572856.1189.qm@web32202.mail.mud.yahoo.com> hello. does anybody has experience using santacruz anti-rat mast cell protease [immunofluorescence] in paraffin embedded tissues? i?m aware that it works fine with frozen tissues but don?t know if it works on paraffin. thanks in advance. felipe beraldo laboratory of nephrology state university of campinas - UNICAMP, Brazil ____________________________________________________________________________________ Veja quais s?o os assuntos do momento no Yahoo! +Buscados http://br.maisbuscados.yahoo.com From Aubrey <@t> nsh.org Thu Jan 21 08:44:23 2010 From: Aubrey <@t> nsh.org (Aubrey Wanner) Date: Thu Jan 21 08:44:29 2010 Subject: [Histonet] NSH One Day VIR Forum Opens Registration Message-ID: Registration is now open for the National Society for Histotechnology One Day Forum in Veterinary, Industry & Research Histology. The Event is scheduled for Saturday, March 27, 2010 in Bethesda, MD, 8:15 am ? 4:45 pm. Registration Fees Member: $119 Non Member: $159 Sessions Include: ? Animal Techniques: Rodent Necropsy ? Traveling the Information Highway: Accessing Literature to Enhance Knowledge of Veterinary Histotechnology ? IHC Cross-reactivity of Human Antibodies in Animal Species ? Decalcification Techniques ? Where do we begin? Working Up a New Antibody in Animal Tissue Sections Speakers include: ? Barbara Munch, BS, LATG, GlaxoSmithKline, Research Triangle Park, NC ? Gayle M. Callis, BS, HT/HTL(ASCP)MT, Bozeman MT ? Denise Long Woodward, MS, HT/HTL(ASCP)QIHC, University of Connecticut, Mansfield Center, CT ? Diane L. Sterchi, MS, HT(ASCP)HTL, Covance, New Palestine, IN ? Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC, Premier Laboratory, LLC, Longmont, CO For registration forms and complete program information please visit the NSH website, www.nsh.org. Aubrey M.J. Wanner Meeting Manager National Society for Histotechnology 10320 Little Patuxent Parkway | Suite 804 Columbia, MD 21044 Main | 443.535.4060 Direct | 443.535.4065 Fax | 443.535.4055 http://www.nsh.org/ Looking for continuing education? Visit the NSH website. ??Please consider the environment before printing this email. From Nikki.Wahlberg <@t> bsci.com Thu Jan 21 09:04:17 2010 From: Nikki.Wahlberg <@t> bsci.com (Wahlberg, Nikki) Date: Thu Jan 21 09:04:18 2010 Subject: [Histonet] Biopsy Processing Schedule Message-ID: <5BF2F9FB24C0DC499CD3C4BB6F7B4481037F90DE@MAPMAIL02.bsci.bossci.com> Hello Histonet, I was wondering if anyone can share their Biopsy Processing Schedule with me? We are a research lab and for the first time we will be processing biopsy specimens. We are concerned that our current processing schedule will dry out the tissue too much. Your help is greatly appreciated. Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. From mpence <@t> grhs.net Thu Jan 21 09:09:52 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jan 21 09:09:56 2010 Subject: [Histonet] Biopsy Processing Schedule In-Reply-To: <5BF2F9FB24C0DC499CD3C4BB6F7B4481037F90DE@MAPMAIL02.bsci.bossci.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D16@is-e2k3.grhs.net> You will need to tell us what type of a tissue processor you will be using? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Thursday, January 21, 2010 9:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy Processing Schedule Hello Histonet, I was wondering if anyone can share their Biopsy Processing Schedule with me? We are a research lab and for the first time we will be processing biopsy specimens. We are concerned that our current processing schedule will dry out the tissue too much. Your help is greatly appreciated. Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nikki.Wahlberg <@t> bsci.com Thu Jan 21 09:11:49 2010 From: Nikki.Wahlberg <@t> bsci.com (Wahlberg, Nikki) Date: Thu Jan 21 09:11:49 2010 Subject: FW: [Histonet] Biopsy Processing Schedule Message-ID: <5BF2F9FB24C0DC499CD3C4BB6F7B4481037F90E0@MAPMAIL02.bsci.bossci.com> Oh Sorry, We have a Leica ASP300. Nikki -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Thursday, January 21, 2010 9:10 AM To: Wahlberg, Nikki; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Biopsy Processing Schedule You will need to tell us what type of a tissue processor you will be using? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Thursday, January 21, 2010 9:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy Processing Schedule Hello Histonet, I was wondering if anyone can share their Biopsy Processing Schedule with me? We are a research lab and for the first time we will be processing biopsy specimens. We are concerned that our current processing schedule will dry out the tissue too much. Your help is greatly appreciated. Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ogv_histech <@t> yahoo.com Thu Jan 21 10:36:54 2010 From: ogv_histech <@t> yahoo.com (oscar gonzalez) Date: Thu Jan 21 10:36:59 2010 Subject: [Histonet] Over baked slides Message-ID: <389042.37720.qm@web53504.mail.re2.yahoo.com> Our drying oven misfunctioned over the weekend, heating up to 200 degrees. Needless to say, our tissue in the?slides that were in the oven got fried. Does anyone know or has a recipe for us to "rescue" some of these slides? From FUNKM <@t> mercyhealth.com Thu Jan 21 10:59:41 2010 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Thu Jan 21 10:59:51 2010 Subject: [Histonet] Leica rep Message-ID: <4B58339D.9B87.00AC.0@mercyhealth.com> Hello, does anyone know who the Leica rep in the midwest states Iowa area ? Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 From FUNKM <@t> mercyhealth.com Thu Jan 21 11:28:28 2010 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Thu Jan 21 11:28:36 2010 Subject: [Histonet] Tumor slides Message-ID: <4B583A5C.9B87.00AC.0@mercyhealth.com> Hello, How are folks working there tumor case slides. We have Tumor review each week and we are working with 5 pathologist that all have different styles of work around for reading cases. One of our time issues are looking and pulling slides for review. Slides drying,slides or in there office. I would appreciate any help as we are like all folks smaller workgroup, faster TNT and looking at how we might change this process. This process with need to be tech driving as our pathologist pretty set in there style of process. any help thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 From mpence <@t> grhs.net Thu Jan 21 11:35:03 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jan 21 11:35:08 2010 Subject: [Histonet] Leica rep In-Reply-To: <4B58339D.9B87.00AC.0@mercyhealth.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D17@is-e2k3.grhs.net> Well, I can tell you this. The reps do monitor this site. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Thursday, January 21, 2010 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica rep Hello, does anyone know who the Leica rep in the midwest states Iowa area ? Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Thu Jan 21 11:55:04 2010 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Jan 21 11:55:55 2010 Subject: [Histonet] Movats on mouse aortas Message-ID: Hi, I have had a problem with "splotchy" elastin staining on certain sections of mouse aorta. I use the Modified Movats and do not use a microwave. But from this one lab some areas of the elastic fibers will retain the hematolylin after differentiation while some areas lose it completely, all in the same section. They fix in 10%NBF and the processing gets done at their lab and I receive the unstained slides. My control does not have this problem. Any ideas? Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From HornHV <@t> archildrens.org Thu Jan 21 11:57:42 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jan 21 11:57:46 2010 Subject: [Histonet] Tumor slides In-Reply-To: <4B583A5C.9B87.00AC.0@mercyhealth.com> References: <4B583A5C.9B87.00AC.0@mercyhealth.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8367D@EMAIL.archildrens.org> Our pathology resident takes care of this for us, along with our transcriptionists who pull slides. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Thursday, January 21, 2010 11:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tumor slides Hello, How are folks working there tumor case slides. We have Tumor review each week and we are working with 5 pathologist that all have different styles of work around for reading cases. One of our time issues are looking and pulling slides for review. Slides drying,slides or in there office. I would appreciate any help as we are like all folks smaller workgroup, faster TNT and looking at how we might change this process. This process with need to be tech driving as our pathologist pretty set in there style of process. any help thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From cmiller <@t> physlab.com Thu Jan 21 12:05:14 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Jan 21 12:05:20 2010 Subject: [Histonet] Reliable Bio repair/ refurbish contact in the Omaha area Message-ID: I am in need of a Reliable Bio repair/ refurbish contact in the Omaha area. I am less than satisfied with my current one, I have a 15 year history with them, but sadly I question their quality and integrity. The poor service is so blatant it is now become a reflection of me in my superior's eyes. I am in need of a refurbished cryostat and bio support for my equipment. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From CIngles <@t> uwhealth.org Thu Jan 21 12:11:15 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Jan 21 12:11:19 2010 Subject: [Histonet] tissue retention times Message-ID: OK, everybody crack open your JCAHO requirement manuals... We are a MOHS lab and were surveyed by Joint Commission about 2 months ago. During the survey, we were cited because we only retain our physical tissue specimens for 24 hours, as they remain fresh and are not fixed. We throw the tissue the following afternoon vs. retaining for the "1 week after microscopic sections are examined and reports are reviewed and signed." (Standard #QC.2.120) Has anyone else (specifically a Mohs lab) been cited for this? We are currently trying to appeal, but have been denied so far. I am having a teleconference with the JCAHO powers that be to try and get the appeal going again, but I'm not the lawyer type. The letter states that their interpretation is that even if the tissue is not regarded as 'gross' tissue, any 'useable' tissue must be retained for a minimum of a week after the case is signed off on.I am thinking on focusing on the 'useable' bit as related to diagnostic value of delayed retained fresh tissue processing i.e. freeze artifact, tissue degredation, inability to perform ancillary testing, etc. Also, what is the definition of 'reviewed and signed reports'? Does this mean when the case is finalized, or just the tissue sections in question? We sometimes have patients that go a month + between Mohs procedures on the same positive lesion. We will probably have to get a -80 freezer for this if the appeal doesn't go through. Not to mention the fact that some of our cases are not resolved for months. We would have constantly search when cases are finalized to know when we can dispose of the tissue. FYI, the letter also states that the JCAHO standard is more stringent than the CLIA standard, so I'm not even really going to use it in arguments. HELP! Claire Ingles UW Hospital & Clinics Madison WI From rjbuesa <@t> yahoo.com Thu Jan 21 12:12:48 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 21 12:12:52 2010 Subject: [Histonet] Over baked slides In-Reply-To: <389042.37720.qm@web53504.mail.re2.yahoo.com> Message-ID: <440592.63574.qm@web65701.mail.ac4.yahoo.com> They are "dead" as an over fried fish or a burnt cookie. Cut them again, they will be essentially worthless, no matter what you do. Ren? J. --- On Thu, 1/21/10, oscar gonzalez wrote: From: oscar gonzalez Subject: [Histonet] Over baked slides To: histonet@lists.utsouthwestern.edu Date: Thursday, January 21, 2010, 11:36 AM Our drying oven misfunctioned over the weekend, heating up to 200 degrees. Needless to say, our tissue in the?slides that were in the oven got fried. Does anyone know or has a recipe for us to "rescue" some of these slides? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Kolman <@t> va.gov Thu Jan 21 12:17:32 2010 From: Kim.Kolman <@t> va.gov (Kolman, Kimberly D.) Date: Thu Jan 21 12:17:36 2010 Subject: [Histonet] tissue retention times In-Reply-To: References: Message-ID: <9C32F30B6662D74A8419DDDB7E66656A0251789C@VHAV15MSGA1.v15.med.va.gov> We are a small Mohs operation, but we just process and paraffin block all our remaining specimens and file them with the rest of our pathology cases. This worked for CAP anyway. Kim Kolman, HT, (ASCP) VA Eastern Kansas Health Care System Leavenworth, Ks 66048 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Thursday, January 21, 2010 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue retention times OK, everybody crack open your JCAHO requirement manuals... We are a MOHS lab and were surveyed by Joint Commission about 2 months ago. During the survey, we were cited because we only retain our physical tissue specimens for 24 hours, as they remain fresh and are not fixed. We throw the tissue the following afternoon vs. retaining for the "1 week after microscopic sections are examined and reports are reviewed and signed." (Standard #QC.2.120) Has anyone else (specifically a Mohs lab) been cited for this? We are currently trying to appeal, but have been denied so far. I am having a teleconference with the JCAHO powers that be to try and get the appeal going again, but I'm not the lawyer type. The letter states that their interpretation is that even if the tissue is not regarded as 'gross' tissue, any 'useable' tissue must be retained for a minimum of a week after the case is signed off on.I am thinking on focusing on the 'useable' bit as related to diagnostic value of delayed retained fresh tissue processing i.e. freeze artifact, tissue degredation, inability to perform ancillary testing, etc. Also, what is the definition of 'reviewed and signed reports'? Does this mean when the case is finalized, or just the tissue sections in question? We sometimes have patients that go a month + between Mohs procedures on the same positive lesion. We will probably have to get a -80 freezer for this if the appeal doesn't go through. Not to mention the fact that some of our cases are not resolved for months. We would have constantly search when cases are finalized to know when we can dispose of the tissue. FYI, the letter also states that the JCAHO standard is more stringent than the CLIA standard, so I'm not even really going to use it in arguments. HELP! Claire Ingles UW Hospital & Clinics Madison WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laura.Miller <@t> leica-microsystems.com Thu Jan 21 12:51:30 2010 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Thu Jan 21 12:51:50 2010 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 01/21/2010 and will not return until 01/22/2010. I am on vacation today. I will respond to you tomorrow when I return. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From greenjumpyone <@t> hotmail.com Thu Jan 21 12:54:35 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Thu Jan 21 12:54:39 2010 Subject: [Histonet] Microwave Tissue Processing In-Reply-To: References: Message-ID: We are getting a microwave tissue processor and the question has come up as to whether or not a lab aide is allowed, per Florida requirements, to run the processors. These processors are semi-automatic and require involvement by staff to go from one step to the next. Does anyone have any experience or knowledge about this type of situation? Thanks! hopper _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/196390708/direct/01/ From cmiller <@t> physlab.com Thu Jan 21 13:11:14 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Jan 21 13:11:20 2010 Subject: [Histonet] tissue retention times In-Reply-To: <9C32F30B6662D74A8419DDDB7E66656A0251789C@VHAV15MSGA1.v15.med.va.gov> References: <9C32F30B6662D74A8419DDDB7E66656A0251789C@VHAV15MSGA1.v15.med.va.gov> Message-ID: We do the same. We aren't a MOHs operation but we do many frozen cases and we process the tissue, cut a permanent processed slide of the frozen sections and then retain them for 10 years along with our other blocks. Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kolman, Kimberly D. Sent: Thursday, January 21, 2010 12:18 PM To: Ingles Claire ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] tissue retention times PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From jreichensperger <@t> siumed.edu Thu Jan 21 13:13:33 2010 From: jreichensperger <@t> siumed.edu (Joel Reichensperger) Date: Thu Jan 21 13:13:40 2010 Subject: [Histonet] Tendon processing question Message-ID: <4B58A75D.9080204@siumed.edu> We are looking for the best way to process tendon (specifically rabbit) that has been severed and then repaired. Currently they are removing the tendon, rinsing it in PBS and then putting it in a cassette in formalin. The problem I am having is that there appears to be some calcification or some other hardening at the area of the repair which is causing the sections to shred when they are cut. We are thinking about using a decalcification agent, but not sure if one is better than another for this type of tissue. We intend to due IHC on the slides once they are cut. Does anyone have any experience with this problem and would be willing to help me out? Thanks, Joel Reichensperger -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) From napoli <@t> siscom.net Thu Jan 21 13:31:50 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Thu Jan 21 13:31:53 2010 Subject: [Histonet] biopsy cassettes Message-ID: <4b58aba6.f.49e6.1720904679@siscom.net> For tiny or friable fragments of skin or keratinaceous aggregate debris or mucoid specimens, one method is to use pieces of "hair curler" wraps to place the tissue in and then place the specimen in regular cassette or in between sponges in cassette. You wont lose your specimen and it will process well. Those wraps are available at drug stores or grocery stores. They are cheap. They are GREAT for tiny derm biopsies >2mm in greatest dimension Regards, Andrew Burgeson histotechnologist From liz <@t> premierlab.com Thu Jan 21 13:44:36 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jan 21 13:44:41 2010 Subject: [Histonet] Tendon processing question In-Reply-To: <4B58A75D.9080204@siumed.edu> Message-ID: I would use formic acid as a decalcification agent, it works well with IHC. We have processed quite a few tendons and we find that you also need a longer processing cycle, 1 - 1.5 hours per station. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger Sent: Thursday, January 21, 2010 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tendon processing question We are looking for the best way to process tendon (specifically rabbit) that has been severed and then repaired. Currently they are removing the tendon, rinsing it in PBS and then putting it in a cassette in formalin. The problem I am having is that there appears to be some calcification or some other hardening at the area of the repair which is causing the sections to shred when they are cut. We are thinking about using a decalcification agent, but not sure if one is better than another for this type of tissue. We intend to due IHC on the slides once they are cut. Does anyone have any experience with this problem and would be willing to help me out? Thanks, Joel Reichensperger -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AJohnson <@t> aipathology.com Thu Jan 21 13:46:52 2010 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Thu Jan 21 13:46:58 2010 Subject: [Histonet] Chemical outdates Message-ID: <704247D5A09D004C9E6B115138D1703A1C4B0B@hpserv001.aipathology.local> Hello Histonetters, We just got inspected by CAP and it was recommended that we put expiration dates on our chemicals once we open them. What kind of time frame do any of you other labs do in regards to this? Thanks for your input, Amylin Johnson From flnails <@t> texaschildrens.org Thu Jan 21 13:50:09 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Jan 21 13:51:53 2010 Subject: [Histonet] RE: Reliable Bio repair/ refurbish contact in the Omaha area In-Reply-To: References: Message-ID: Have you tried Tom Buck with Hawkeye biomedical -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, January 21, 2010 12:05 PM To: histonet Subject: [Histonet] Reliable Bio repair/ refurbish contact in the Omaha area I am in need of a Reliable Bio repair/ refurbish contact in the Omaha area. I am less than satisfied with my current one, I have a 15 year history with them, but sadly I question their quality and integrity. The poor service is so blatant it is now become a reflection of me in my superior's eyes. I am in need of a refurbished cryostat and bio support for my equipment. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From MSHERWOOD <@t> PARTNERS.ORG Thu Jan 21 14:02:53 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Jan 21 14:03:01 2010 Subject: [Histonet] Tendon processing question In-Reply-To: References: <4B58A75D.9080204@siumed.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F6C@PHSXMB30.partners.org> Liz, In that regard, we just received mice tibia in formalin for IHC. We need to do decalcification. Do you recommend formic acid (could you give us the procedure)and how long in the formic acid? We do very little decal and have had problems in the past with it being incomplete. Any help would be greatly appreciated! Thanks! Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, January 21, 2010 2:45 PM To: Joel Reichensperger; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tendon processing question I would use formic acid as a decalcification agent, it works well with IHC. We have processed quite a few tendons and we find that you also need a longer processing cycle, 1 - 1.5 hours per station. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger Sent: Thursday, January 21, 2010 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tendon processing question We are looking for the best way to process tendon (specifically rabbit) that has been severed and then repaired. Currently they are removing the tendon, rinsing it in PBS and then putting it in a cassette in formalin. The problem I am having is that there appears to be some calcification or some other hardening at the area of the repair which is causing the sections to shred when they are cut. We are thinking about using a decalcification agent, but not sure if one is better than another for this type of tissue. We intend to due IHC on the slides once they are cut. Does anyone have any experience with this problem and would be willing to help me out? Thanks, Joel Reichensperger -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From pruegg <@t> ihctech.net Thu Jan 21 14:37:49 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Jan 21 14:38:35 2010 Subject: [Histonet] Slide writer In-Reply-To: <63EA0607835FBA4689CEA9EA8B48269202B8675D@usctmx1141.merck.com> References: <50003EC02E2CEA4583BEB3CD08EAC1E090DD87@EXCHANGEBE2.carle.com> <0BA1412CBA284B528A9233134F84A7F5@prueggihctechlt> <63EA0607835FBA4689CEA9EA8B48269202B8675D@usctmx1141.merck.com> Message-ID: <5F5E76315D5C4F09AC0605911C84996D@Patsyoffice> It writes directly on the slide. Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Connolly, Brett M [mailto:brett_connolly@merck.com] Sent: Thursday, January 21, 2010 7:06 AM To: Patsy Ruegg Subject: RE: [Histonet] Slide writer Hi Patsy, Does the Leica slide labeler write directly on the frosted slide or does it print out sticky labels? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, January 21, 2010 8:41 AM To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide writer In our new Derm Path lab we went with Leica for slide labeling and block labeling, we designed our own computer program with bar coding and other identifiers and the labelers plugged right into our system, they are not cheap but we really like them. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, January 20, 2010 6:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide writer Hello Histonetters! I know this has been discussed before but at the time we did not need this instrument so I didn't pay much attention to the posts. So here is my request. We need a new slide writer. We currently have a Thermo Electron Microwriter which must be a lemon because we have had nothing but problems with it. We want to replace this instrument with one that we can count on. So please all you labs out there that have a slide writer let me know what kind of slide writer you use and how it has performed for you. Thanks so much. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From amylee779 <@t> yahoo.com Thu Jan 21 16:19:35 2010 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Thu Jan 21 16:19:38 2010 Subject: [Histonet] To detect injected mouse antibody inside mouse tissue Message-ID: <986552.34322.qm@web38008.mail.mud.yahoo.com> Hello, ? I have a question regarding detecting injected mouse antibody in mouse tissue by IHC. Now I have mouse liver paraffin section. This mouse was injected mouse antibody. I was asked to locate this antibody. This is a mouse chimeric antibody. Variable regions from human antibody was combined with mouse IgG2a heavy chain and kappa light chain constant regions. I am thinking using rabbit anti-human IgG, F(ab) fragment specific. Do you think it's doable or you have any good suggestion? When this mouse antibody bind to antigen inside liver through this human?variable region, my F(ab) fragment specific antibody can still bind to it? ? Hope I describ my questions clearly. ? Thank you in advance for any help! ? Amy From traczyk7 <@t> aol.com Thu Jan 21 17:05:03 2010 From: traczyk7 <@t> aol.com (traczyk7@aol.com) Date: Thu Jan 21 17:05:37 2010 Subject: [Histonet] Glyoxol vs Formalin Message-ID: <146cc.87c0bf3.388a379f@aol.com> What changes should one expect to do when changing from a glyoxol fixative to formalin? Client has been successfully processing prostate biopsies on a Milestone RHS for several years. They are now looking to switch to formalin in anticipation of the need for future molecular studies. The nuclear detail on their trial run formalin fixed (4 hours pre-mw) is not good. Do you think a formalin-alcohol mixture would give them results closer to what they got with Glyoxol? Thanks in advance. Dorothy Dorothy Traczyk MTA Histology PO Box 602 Point Pleasant, NJ 08742 T: 732-899-2912 F: 732-899-5469 dorothy@mtahistology.com From koellingr <@t> comcast.net Thu Jan 21 17:19:08 2010 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Jan 21 17:19:11 2010 Subject: [Histonet] To detect injected mouse antibody inside mouse tissue In-Reply-To: <1644688440.13001811264115448522.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: <1102621174.13005391264115948112.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Amy, In my opinion the answer is, Yes, certainly possible.? Also in my opinion the answer is No, probably impossible.? Even with a Yahoo address I'm assuming that this is a research/biotech model and project.? In a previous life, when?quietly gagged, I went about this on numerable occassions.? Sometimes extraordinarily successfully and sometimes complete failures.? Done at both light IHC level and with EM IHC.? Depends on your specific antibody molecule, how many target receptors on a given cell, pharmacokinetics of the injected antibody, making sure it gets to target and in sufficient quantity, not sequestered inappropriately, how long animal sacrificed post-injection.? Probably 5 other variables, each with nuances so just impossible to answer generally in this venue for your specific problem.? There are several papers on the subect if you look them up but they are specific procedures for a very specific model.? If successful, results are spectacular.? More often than not, you spend months on something that in retrospect was probably not doable in the first place after you sit and really think it through.? I once got bitten to the tune of several months work and at the end we found out something about the glycosylation of the antibody that would never allow us to localize it appropriately so we had been searching for something we could never find by IHC. Ray Raymond Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Amy Lee" To: "histonet" Sent: Thursday, January 21, 2010 2:19:35 PM GMT -08:00 US/Canada Pacific Subject: [Histonet] To detect injected mouse antibody inside mouse tissue Hello, ? I have a question regarding detecting injected mouse antibody in mouse tissue by IHC. Now I have mouse liver paraffin section. This mouse was injected mouse antibody. I was asked to locate this antibody. This is a mouse chimeric antibody. Variable regions from human antibody was combined with mouse IgG2a heavy chain and kappa light chain constant regions. I am thinking using rabbit anti-human IgG, F(ab) fragment specific. Do you think it's doable or you have any good suggestion? When this mouse antibody bind to antigen inside liver through this human?variable region, my F(ab) fragment specific antibody can still bind to it? ? Hope I describ my questions clearly. ? Thank you in advance for any help! ? Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sue.reilly <@t> jcu.edu.au Thu Jan 21 17:35:51 2010 From: sue.reilly <@t> jcu.edu.au (Sue Reilly) Date: Thu Jan 21 17:36:05 2010 Subject: [Histonet] tamponated formaldehyde In-Reply-To: <201001220421.AEP44224@jcu.edu.au> References: <201001220421.AEP44224@jcu.edu.au> Message-ID: <201001212335.HCZ95113@jcu.edu.au> >Hi; >Can someone please explain to me what is meant by tamponated formaldehyde ? Thanks Sue Sue Reilly Histechnologist. Histology Unit. School of Marine & Tropical Biology. James Cook University,Townsville. QLD. 4811. AUSTRALIA P (07) 4781 4181 F (07) 4725 1570 E sue.reilly@jcu.edu.au www.jcu.edu.au Location: Building DB 028, Room 106. JCU CRICOS Provider Code: 00117J Important Notice: The contents of this email transmission, including any attachments, are intended solely for the named addressee and are confidential; any unauthorised use, reproduction or storage of the contents and any attachments is expressly prohibited. If you have received this transmission in error please delete it and any attachments from your system immediately and advise the sender by return email or telephone. James Cook University does not warrant that this email and any attachments are error or virus free. From Timothy.Morken <@t> ucsfmedctr.org Thu Jan 21 17:54:57 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Jan 21 17:55:05 2010 Subject: [Histonet] tamponated formaldehyde In-Reply-To: <201001212335.HCZ95113@jcu.edu.au> References: <201001220421.AEP44224@jcu.edu.au> <201001212335.HCZ95113@jcu.edu.au> Message-ID: <1AAF670737F193429070841C6B2ADD4C0121B26D45@EXMBMCB15.ucsfmedicalcenter.org> They mean "buffered." It's a poor translation from Italian/Spanish/portugese (BTW, found this answer on Histonet!!) Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Reilly Sent: Thursday, January 21, 2010 3:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tamponated formaldehyde >Hi; >Can someone please explain to me what is meant by tamponated formaldehyde ? Thanks Sue Sue Reilly Histechnologist. Histology Unit. School of Marine & Tropical Biology. James Cook University,Townsville. QLD. 4811. AUSTRALIA P (07) 4781 4181 F (07) 4725 1570 E sue.reilly@jcu.edu.au www.jcu.edu.au Location: Building DB 028, Room 106. JCU CRICOS Provider Code: 00117J Important Notice: The contents of this email transmission, including any attachments, are intended solely for the named addressee and are confidential; any unauthorised use, reproduction or storage of the contents and any attachments is expressly prohibited. If you have received this transmission in error please delete it and any attachments from your system immediately and advise the sender by return email or telephone. James Cook University does not warrant that this email and any attachments are error or virus free. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DixonM <@t> vetmed.ufl.edu Fri Jan 22 07:31:55 2010 From: DixonM <@t> vetmed.ufl.edu (MaryAnn Dixon) Date: Fri Jan 22 07:32:00 2010 Subject: [Histonet] xylene alternatives Message-ID: <530D827EC657DE418C3572ADD63FCDC30151240D@EXGVMCNETWORK.vetmed.ufl.edu> Hi Histonetters, I would like to start using a xylene alternative in my stain line but are unfamiliar with the latest products. I've heard of slidebrite and propar only. All opinions and suggestions are greatly appreciated. Thanks! MaryAnn MaryAnn Dixon BS, HT (ASCP)cm Biological Scientist Surgical Oncology UF College of Veterinary Medicine Phone (352) 294-4516 From LSebree <@t> uwhealth.org Fri Jan 22 08:10:10 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Jan 22 08:12:31 2010 Subject: [Histonet] Reference lab for "Pro Ex C" Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF748@UWHC-MAIL01.uwhis.hosp.wisc.edu> Hello histonet, We are in immediate need of a reference lab that performs Pro Ex C on human FFPE tissue. Thank you, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From rjbuesa <@t> yahoo.com Fri Jan 22 08:43:55 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 22 08:43:59 2010 Subject: [Histonet] tamponated formaldehyde In-Reply-To: <201001212335.HCZ95113@jcu.edu.au> Message-ID: <483585.42451.qm@web65703.mail.ac4.yahoo.com> Sue: In Spanish, French and Italian a buffer solution is called a "tampon" solution. So "tamponated", which is a word nonexistent in English, "wants" to mean a "buffered" solution. Ren? J. --- On Thu, 1/21/10, Sue Reilly wrote: From: Sue Reilly Subject: [Histonet] tamponated formaldehyde To: histonet@lists.utsouthwestern.edu Date: Thursday, January 21, 2010, 6:35 PM > Hi; > Can someone please explain to me what is meant by tamponated formaldehyde ? Thanks Sue Sue Reilly Histechnologist. Histology Unit. School of Marine & Tropical Biology. James Cook University,Townsville. QLD. 4811. AUSTRALIA P (07) 4781 4181 F (07) 4725 1570 E sue.reilly@jcu.edu.au www.jcu.edu.au Location: Building DB 028, Room 106. JCU CRICOS Provider Code: 00117J Important Notice: The contents of this email transmission, including any attachments, are intended solely for the named addressee and are confidential; any unauthorised use, reproduction or storage of the contents and any attachments is expressly prohibited. If you have received this transmission in error please delete it and any attachments from your system immediately and advise the sender by return email or telephone. James Cook University does not warrant that this email and any attachments are error or virus free. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From remy.lopez <@t> applieddiagnostics.com Fri Jan 22 10:31:06 2010 From: remy.lopez <@t> applieddiagnostics.com (Remy Lopez) Date: Fri Jan 22 10:31:00 2010 Subject: [Histonet] FISH Qualifications/Training Message-ID: <000301ca9b80$4d55aca0$e80105e0$@lopez@applieddiagnostics.com> Good Morning! Does anyone know what are the requirements or qualifications for people to be able to read HER 2 FISH slides? Does it have to be a pathologist? Are there any courses/training available for this? Thanks Remy From rjbuesa <@t> yahoo.com Fri Jan 22 10:56:51 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 22 10:56:56 2010 Subject: [Histonet] FISH Qualifications/Training In-Reply-To: <000301ca9b80$4d55aca0$e80105e0$@lopez@applieddiagnostics.com> Message-ID: <894050.9685.qm@web65705.mail.ac4.yahoo.com> Usually counting is done by a trained histotechnologist. Some companies, like Abbott, have training/certification programs. A pathologist always has to interprete the results, and sign the case. Ren? J. ? ? --- On Fri, 1/22/10, Remy Lopez wrote: From: Remy Lopez Subject: [Histonet] FISH Qualifications/Training To: histonet@lists.utsouthwestern.edu Date: Friday, January 22, 2010, 11:31 AM Good Morning! Does anyone know what are the requirements or qualifications for people to be able to read HER 2 FISH slides?? Does it have to be a pathologist? Are there any courses/training available for this? Thanks Remy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Fri Jan 22 12:36:05 2010 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Fri Jan 22 12:37:16 2010 Subject: [Histonet] Joel - re: tendons Message-ID: <001001ca9b91$c1d315c0$c5d76880@vetmed.wisc.edu> Joel: Increase all stations of processing time, as Liz suggested. For large sections of tendon, or when looking at length with both tendon attachments, I process by hand and soak in paraffin changes over-night (oven). Unless you have a bone attachment, decalcification should be unnecessary. Tendons are challenging. The smaller the tissue size, the easier to work with and to get thinner, better quality sections. Vicki From cmiller <@t> physlab.com Fri Jan 22 12:47:39 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Jan 22 12:47:46 2010 Subject: [Histonet] re;Biomedical repair Message-ID: Thanks to all of you who responded. I had no idea I would catch so many peoples attention, especially Vendors (yikes). I will think twice before I send another one for sure. I have heard good things about Hawkeye Biomedical from many of you and they are currently working with me on getting what I need. Have a great weekend!!! Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From liz <@t> premierlab.com Fri Jan 22 12:49:45 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jan 22 12:49:50 2010 Subject: [Histonet] Joel - re: tendons In-Reply-To: <001001ca9b91$c1d315c0$c5d76880@vetmed.wisc.edu> Message-ID: Vicki is correct for larger samples I process manually with graded alcohols - overnight for each step till I get to the last 100% alcohol, I do 3 100% for larger samples (we process large 2 x 3 tissue both soft tissue and bone routinely). You might think that these tissues will be over processed but we have never really experienced that. What you don't want to have is an under processed sample. I have a processing cycle on the processor that's 6 hours per station, for the last alcohol, xylenes and paraffins (I also use 3 xylenes and 4 paraffin stations). Vicki is correct - tendon can be challenging. The reason we manually process is that we can't tie up our tissue processor that much for these samples. We only have one. But we have lots of different processing cycles for all types of tissue. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vicki Kalscheur Sent: Friday, January 22, 2010 11:36 AM To: Histonet Discussion Subject: [Histonet] Joel - re: tendons Joel: Increase all stations of processing time, as Liz suggested. For large sections of tendon, or when looking at length with both tendon attachments, I process by hand and soak in paraffin changes over-night (oven). Unless you have a bone attachment, decalcification should be unnecessary. Tendons are challenging. The smaller the tissue size, the easier to work with and to get thinner, better quality sections. Vicki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa.ribeiro <@t> brinegroup.com Fri Jan 22 14:56:27 2010 From: melissa.ribeiro <@t> brinegroup.com (Melissa Ribeiro) Date: Fri Jan 22 14:56:33 2010 Subject: [Histonet] Histology Manager job opportunity Message-ID: <43904A2EECEAB54D8A023931049FEA4C218D17@brin-sbs01.brinegroup.local> My client, a 300-bed hospital in Southeastern Connecticut has an immediate opening for a HISTOLOGY MANAGER. The Histology Manager is responsible for managing and supervising 2 full-time Histology staff members, and ensuring that the department runs effectively. Additional responsibilities include: - Managing department budget - Hiring/firing personnel, conducting employee reviews - Participating in intra-departmental QA/QC meetings - Ensuring compliance with all department reviews - Troubleshooting and resolving all technical issues Requirements - Bachelors degree in Biology or related field - Minimum 8 years of experience in a Histology Laboratory - HT or HTL (ASCP) certification - Management experience from a Clinical hospital-based or reference lab This facility will pay for interviewing expenses and also provide relocation assistance. Interested candidates, please contact MELISSA RIBEIRO PASSOS at 781-272-3400 ext.228 or via email at mribeiro@brinegroup.com Melissa Ribeiro Passos Healthcare Division Brine Group Staffing Solutions 20 Mall Road, Suite 225 Burlington, MA 01803 mribeiro@brinegroup.com Ph. (781) 272-3400 ext. 228 Fax (781) 494-3401 From JPeters <@t> bostwicklaboratories.com Fri Jan 22 15:13:25 2010 From: JPeters <@t> bostwicklaboratories.com (Justin Peters) Date: Fri Jan 22 15:13:30 2010 Subject: [Histonet] IHC stain for TFEB Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F09F45894@mail1.BOSTWICK.COM> Does anyone know any reference labs that would be able to perform an IHC stain for TFEB? Justin From Kimberly.Marshall <@t> ahss.org Fri Jan 22 15:26:03 2010 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Fri Jan 22 15:26:18 2010 Subject: [Histonet] Wright-Giemsa stain Message-ID: I am from a small Hospital where due to the size, Histology does the Bone Marrow's. We go to the procedure, make the smears, as well as do the Wright-Giemsa stain. I am having a hard time getting the Wright's stain dark enough for my Pathologist. I have added stain time, checked the pH of my stain, tried different rinse's and buffers, but still not getting it dark enough. I do go back to the stain for 45 mins or more and that will get it at least close to what they want. Is there anyone out there that can give me some advise on this stain? Thanks in advance for your time Kimberly ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From gu.lang <@t> gmx.at Sat Jan 23 03:27:54 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Jan 23 03:28:03 2010 Subject: AW: [Histonet] FISH Qualifications/Training In-Reply-To: <000301ca9b80$4d55aca0$e80105e0$@lopez@applieddiagnostics.com> References: <000301ca9b80$4d55aca0$e80105e0$@lopez@applieddiagnostics.com> Message-ID: Here it depends on the cases and the type of probes, that have to be read. In some pathology departments in Austria Her2neu is counted by histotechnologists, formerly trained by pathologists. Hematology probes for translocations in lymphomas, or FISH in difficult tissues like sarcomas are read by pathologists. Because of the time consuming work there's a trend, that more histotechnologists should be trained for counting. But in the end the doctor has to sign the report. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Remy Lopez Gesendet: Freitag, 22. J?nner 2010 17:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH Qualifications/Training Good Morning! Does anyone know what are the requirements or qualifications for people to be able to read HER 2 FISH slides? Does it have to be a pathologist? Are there any courses/training available for this? Thanks Remy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Jan 23 14:26:33 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Jan 23 14:26:40 2010 Subject: [Histonet] Re: Wright-Giemsa stain Message-ID: Kimberly Marshall asks: >>I am from a small hospital where due to the size, Histology does the Bone Marrows. We go to the procedure, make the smears, as well as do the Wright-Giemsa stain. I am having a hard time getting the Wright's stain dark enough for my pathologist. I have added stain time, checked the pH of my stain, tried different rinses and buffers, but still not getting it dark enough. I do go back to the stain for 45 minutes or more and that will get it at least close to what they want. Is there anyone out there that can give me some advise on this stain?<< There are many possibilities. First thing I'd do is get a new bottle of stain, preferably from a different source. Very small amounts of water contaminating the stock bottle are ruinous. Bob Richmond Samurai Pathologist Knoxville TN From liz <@t> premierlab.com Sat Jan 23 14:32:00 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sat Jan 23 14:32:04 2010 Subject: [Histonet] Re: Wright-Giemsa stain In-Reply-To: Message-ID: One other thing to be aware of is any formaldehyde fumes, you need to keep the smear samples away from formalin as much as possible. If the bone marrow smears come in contact with formalin fumes they will not stain properly. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Saturday, January 23, 2010 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Wright-Giemsa stain Kimberly Marshall asks: >>I am from a small hospital where due to the size, Histology does the Bone Marrows. We go to the procedure, make the smears, as well as do the Wright-Giemsa stain. I am having a hard time getting the Wright's stain dark enough for my pathologist. I have added stain time, checked the pH of my stain, tried different rinses and buffers, but still not getting it dark enough. I do go back to the stain for 45 minutes or more and that will get it at least close to what they want. Is there anyone out there that can give me some advise on this stain?<< There are many possibilities. First thing I'd do is get a new bottle of stain, preferably from a different source. Very small amounts of water contaminating the stock bottle are ruinous. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kc <@t> ka-recruiting.com Sat Jan 23 15:00:58 2010 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Sat Jan 23 15:01:05 2010 Subject: [Histonet] New Histology Jobs! Message-ID: <1631942468.1264280458045.JavaMail.cfservice@webserver51> Hi Histoneters, Are you interested in hearing about new job opportunities? I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. Below is a list of some of the other Histology opportunities we are currently working on. Histotechs/Cytotechs: ****Northern, VA - Histotechnologist 2nd shift **** New Opening!!! ****Las Vegas, NV - Histology Supervisor **** New Opening!!! Connecticut- Histology Operations Manager Southern CA - Histology Supervisor New York City - Surgical Pathology and Histology Supervisor New York City - Histotech 3rd shift Las Vegas, NV - Histotech 3rd shift Central Georgia - Histotech 1st shift Oklahoma - Histotech 1st shift (with opportunity to be promoted to supervisor) Long Island, NY - Cytotech New York City - Cytotech Pennsylvania - Cytology Supervisor Connecticut ? Cytotech If you're interested in learning more about any of these opportunities then please email me a resume and let me know how best to get in touch with you. If none of these are a fit please let me know what you'd be interested in and where you're looking so I can tailor a search for you. With the New Year upon us many of our clients have fresh hiring budgets and will be looking to add people over the next several months. We work on positions at all levels and cover the entire US. To view some additional opportunities please visit our website at www.ka-recruiting.com . Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From estellamireles <@t> gmail.com Sat Jan 23 18:26:23 2010 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Sat Jan 23 18:26:29 2010 Subject: [Histonet] PT Histology Job Houston, Tx (nights) Message-ID: A group of endo. doctors are looking for one or two histotechs to gross, process, cut and stain tissues at their private lab, located in West Houston. The job will start late afternoon. If interested contact me . From pathmaster <@t> yahoo.com Sun Jan 24 12:38:45 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Sun Jan 24 12:38:50 2010 Subject: [Histonet] What's wrong with Diff Quick? Message-ID: <42168.1077.qm@web111110.mail.gq1.yahoo.com> Why mess with persnickety Wright Giemsa stains. We stain all our bone marrows, lymph node touch preps etc with Diff Quick- the same set up we use for our H pylori biopsies minus the fixative step. Can someone tell me the difference if there is any and why anyone would mess with Wright Giemsa when Diff Quick (or others like it) are so easy? Our docs are just fine with this. Another alternative we used to do was put the BM smear? on the stainer in Hematology. Worked fine too. ? Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant Southside Hospital NSLIJHS From cfrmd1 <@t> gmail.com Sun Jan 24 12:44:03 2010 From: cfrmd1 <@t> gmail.com (Carlos Rodriguez, MD) Date: Sun Jan 24 12:44:11 2010 Subject: [Histonet] Histotech hourly rate in Scottsdale, AZ Message-ID: <65c42edc1001241044k3221db1l1b61a3ef47d7e2ce@mail.gmail.com> Dear histonet.org members- Could anyone please give me an approximate hourly wage for a part-time dermpath histotech in Scottsdale, AZ? The setting would be a private practice dermatology clinic with an in-house lab. The histotech would be cutting and staining skin specimens only (shave and punch biopsies & small excisions) a few days/week. Thanks very much! Carlos Rodriguez, MD From KPercival <@t> wyeth.com Mon Jan 25 07:46:10 2010 From: KPercival <@t> wyeth.com (Percival Karen) Date: Mon Jan 25 07:46:20 2010 Subject: [Histonet] Wright-Giemsa stain In-Reply-To: References: Message-ID: <4B5D5A5202000011001F7EC4@gv01a67m.gv.us.pri.wyeth.com> Kim, how are you fixing the smear? Make sure to use acetone-free methanol. Are you using a manufactured stain kit? If so, which one? Karen Andover MA >>> "Marshall, Kimberly" 1/22/2010 4:26 PM >>> I am from a small Hospital where due to the size, Histology does the Bone Marrow's. We go to the procedure, make the smears, as well as do the Wright-Giemsa stain. I am having a hard time getting the Wright's stain dark enough for my Pathologist. I have added stain time, checked the pH of my stain, tried different rinse's and buffers, but still not getting it dark enough. I do go back to the stain for 45 mins or more and that will get it at least close to what they want. Is there anyone out there that can give me some advise on this stain? Thanks in advance for your time Kimberly ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Mon Jan 25 08:58:19 2010 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Jan 25 08:58:25 2010 Subject: [Histonet] Artisan Message-ID: <4B5D6B3B.2B7F.00C9.0@geisinger.edu> Anyone know the approx. price of Dakos Artisan? Cost per slide? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From jkiernan <@t> uwo.ca Mon Jan 25 10:02:38 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Jan 25 10:02:48 2010 Subject: [Histonet] Wright-Giemsa stain Message-ID: Using a combination of two traditional blood stains such as Giemsa's and Wright's seems an antiquated approach. According to G. Clark's "Staining Procedures" (3rd ed, 1973, p.131) it dates from Artur Pappenheim (1912). It is certainly irrational to use in succession two stains that ought to be closely similar. The staining mechanism for this group of methods has been pretty well understood for more than 30 years. All the desired colours can be obtained using a solution containing just two dyes (azure B and eosin Y) that are available as pure compounds. There are simple staining methods (eg the "standardized Romanowsky-Giemsa method" in Boon & Drijver's "Routine Cytological Staining Techniques", 1986) that use only the two pure dyes. Good eosin Y with high purity, has been around for years. Pure azure B (made by direct synthesis and sold as azure B isothiocyanate) is expensive. It is not the same as certified azure B, which is made by partial demethylation of methylene blue and also contains some azure A and methylene blue (see Penney et al. 2002 Biotech. Histochem. 77: 237-275.). Certified dyes are powders that have been tested by an independent non-profit organization, the Biological Stain Commission (BSC) and are used by vendors of solutions. In addition to methylene blue, azures A, B & C, methylene violet (Bernthsen) and eosins Y and B, the BSC also certifies mixed dye powders for some of the traditional blood stains: Giemsa's, Wright's, Jenner's and Macneal's tetrachrome. Ideally, a standardized Romanowsky-Giemsa method with pure azure B and eosin Y should be used, as described by Wittekind & Kretschmer 1987 Histochem. J. 19: 399-401. It is cheaper to use a traditional blood stain, which always contains various unnecessary thiazine dyes in addition to azure B, provided that the stock solution has been made from certified ingredients. The BSC does not certify staining solutions, but the vendors of solutions should state that their products were made using certified dyes. For rational troubleshooting, see Horobin and Walter 1987 "Understanding Romanowsky-Giemsa staining" Histochemistry 86: 331-336 or the book "Troubleshooting Histology Stains" by RW Horobin & JD Bancroft (1998). John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Marshall, Kimberly" Date: Friday, January 22, 2010 16:27 Subject: [Histonet] Wright-Giemsa stain To: histonet@lists.utsouthwestern.edu > I am from a small Hospital where due to the size, Histology does the > Bone Marrow's. We go to the procedure, make the smears, as > well as do > the Wright-Giemsa stain. I am having a hard time > getting the Wright's > stain dark enough for my Pathologist. I have added > stain time, checked > the pH of my stain, tried different rinse's and buffers, but > still not > getting it dark enough. I do go back to the stain for 45 > mins or more > and that will get it at least close to what they want. Is > there anyone > out there that can give me some advise on this > stain? Thanks in > advance for your time > > Kimberly From DKBoyd <@t> chs.net Mon Jan 25 10:11:13 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Mon Jan 25 10:10:30 2010 Subject: [Histonet] Wright-Giemsa stain In-Reply-To: Message-ID: We also do the bone marrow assisting/staining etc. We stain our smears on a Wescor AeroSpray slide stainer. We use the Rapid program which is a 5 min program. But, we have to stain them twice to get the correct intensity that our pathologist prefer. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Marshall, Kimberly" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/22/2010 04:27 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Wright-Giemsa stain I am from a small Hospital where due to the size, Histology does the Bone Marrow's. We go to the procedure, make the smears, as well as do the Wright-Giemsa stain. I am having a hard time getting the Wright's stain dark enough for my Pathologist. I have added stain time, checked the pH of my stain, tried different rinse's and buffers, but still not getting it dark enough. I do go back to the stain for 45 mins or more and that will get it at least close to what they want. Is there anyone out there that can give me some advise on this stain? Thanks in advance for your time Kimberly ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From schaundrawalton <@t> yahoo.com Mon Jan 25 10:26:05 2010 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Mon Jan 25 10:26:08 2010 Subject: [Histonet] PAS on Bone Marrows Message-ID: <162947.47056.qm@web58905.mail.re1.yahoo.com> One of my pathologists is requesting that we define a protocol to start staining bone marrow aspirate smears with PAS.? Is anyone out there doing this??Can you share a protocol (we use the Ventana Nexus special stainer)?? What are you using for controls in this situation? ? Thanks for your help, Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL From rjbuesa <@t> yahoo.com Mon Jan 25 10:43:05 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 25 10:43:09 2010 Subject: [Histonet] PAS on Bone Marrows In-Reply-To: <162947.47056.qm@web58905.mail.re1.yahoo.com> Message-ID: <682391.76660.qm@web65713.mail.ac4.yahoo.com> Try the same protocol you use in the stainer for BM core biopsy. Ren? J. --- On Mon, 1/25/10, Schaundra Walton wrote: From: Schaundra Walton Subject: [Histonet] PAS on Bone Marrows To: "Histonet" Date: Monday, January 25, 2010, 11:26 AM One of my pathologists is requesting that we define a protocol to start staining bone marrow aspirate smears with PAS.? Is anyone out there doing this??Can you share a protocol (we use the Ventana Nexus special stainer)?? What are you using for controls in this situation? ? Thanks for your help, Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Mon Jan 25 11:05:17 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Mon Jan 25 11:05:35 2010 Subject: [Histonet] tissue retention times In-Reply-To: Message-ID: We are not a MOHS lab. I know many laws have changed since I worked in one, this being one of them I think. I would not go as far as to getting a -80 freezer for this. If you cant send them out to another lab for processing or do it there, both which are very costly. I might try saving the samples in the small 20 ml 10% buffered formalin bottles. Not sure what your laws are there about the disposal of formalin but here we can neutralize it and toss it. So this might be a cost efficient way for you to meet this requirement without processing your samples. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Ingles Claire " Sent by: histonet-bounces@lists.utsouthwestern.edu 01/21/2010 12:11 PM To cc Subject [Histonet] tissue retention times OK, everybody crack open your JCAHO requirement manuals... We are a MOHS lab and were surveyed by Joint Commission about 2 months ago. During the survey, we were cited because we only retain our physical tissue specimens for 24 hours, as they remain fresh and are not fixed. We throw the tissue the following afternoon vs. retaining for the "1 week after microscopic sections are examined and reports are reviewed and signed." (Standard #QC.2.120) Has anyone else (specifically a Mohs lab) been cited for this? We are currently trying to appeal, but have been denied so far. I am having a teleconference with the JCAHO powers that be to try and get the appeal going again, but I'm not the lawyer type. The letter states that their interpretation is that even if the tissue is not regarded as 'gross' tissue, any 'useable' tissue must be retained for a minimum of a week after the case is signed off on.I am thinking on focusing on the 'useable' bit as related to diagnostic value of delayed retained fresh tissue processing i.e. freeze artifact, tissue degredation, inability to perform ancillary testing, etc. Also, what is the definition of 'reviewed and signed reports'? Does this mean when the case is finalized, or just the tissue sections in question? We sometimes have patients that go a month + between Mohs procedures on the same positive lesion. We will probably have to get a -80 freezer for this if the appeal doesn't go through. Not to mention the fact that some of our cases are not resolved for months. We would have constantly search when cases are finalized to know when we can dispose of the tissue. FYI, the letter also states that the JCAHO standard is more stringent than the CLIA standard, so I'm not even really going to use it in arguments. HELP! Claire Ingles UW Hospital & Clinics Madison WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From Sandra.Harrison3 <@t> va.gov Mon Jan 25 12:53:43 2010 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Mon Jan 25 12:53:38 2010 Subject: [Histonet] Chemical outdates In-Reply-To: <704247D5A09D004C9E6B115138D1703A1C4B0B@hpserv001.aipathology.local> References: <704247D5A09D004C9E6B115138D1703A1C4B0B@hpserv001.aipathology.local> Message-ID: If there are no expiration dates on the chemical: For chemicals distributed by Fisher, look at the Lot #. The first 2 numbers are the year it was manufactured. In general, chemicals are considered expired 3-5 years after the year they were manufactured. You can also check the certificate that comes when the chemical is delivered. It should have an expiration date or at least a year of manufacture. Sandy Harrison -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Thursday, January 21, 2010 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chemical outdates Hello Histonetters, We just got inspected by CAP and it was recommended that we put expiration dates on our chemicals once we open them. What kind of time frame do any of you other labs do in regards to this? Thanks for your input, Amylin Johnson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NLinke <@t> mednet.ucla.edu Mon Jan 25 13:05:01 2010 From: NLinke <@t> mednet.ucla.edu (Linke, Noelle) Date: Mon Jan 25 13:05:35 2010 Subject: [Histonet] H&E stainer/coverslippers Message-ID: <0C96F0BFE078D74C91A1C541D24A6AE49CB92CA3@EMGMB1.ad.medctr.ucla.edu> Hi all, Is anyone using a slide stainer and coverslipper combo which is NOT Sakura that they are pleased with? The new Sakura coverslipper is enormous and too big for the needs of the smaller lab we are setting up. Thank you! Noelle No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services UCLA Department of Pathology and Laboratory Medicine Phone: 310-825-7397 Fax: 310-983-3289 nlinke@mednet.ucla.edu Pager: 97471 ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From aevans <@t> wellspan.org Mon Jan 25 13:41:33 2010 From: aevans <@t> wellspan.org (Evans, Andria B) Date: Mon Jan 25 13:41:40 2010 Subject: [Histonet] Mucicarmine Stains Message-ID: We are having some issues with our Muci stain being pale. I would like to know what everyone in Histoland is using for the Muci Stain. Thanks! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From contact <@t> excaliburpathology.com Mon Jan 25 13:47:28 2010 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Jan 25 13:51:14 2010 Subject: [Histonet] Crystal Mount Alternative Message-ID: <288021.68424.qm@web1116.biz.mail.sk1.yahoo.com> Hello, is anyone using the Crystal Mount alternative? Is it as good as the original? Paula Pierce, BA, HTL(ASCP)HT President Excalibur Pathology 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From godsgalnow <@t> aol.com Mon Jan 25 14:12:51 2010 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Jan 25 14:12:43 2010 Subject: [Histonet] Mucicarmine Stains In-Reply-To: References: Message-ID: <531434241-1264450358-cardhu_decombobulator_blackberry.rim.net-1830481601-@bda112.bisx.prod.on.blackberry> We use Biocare Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Evans, Andria B" Date: Mon, 25 Jan 2010 14:41:33 To: Subject: [Histonet] Mucicarmine Stains We are having some issues with our Muci stain being pale. I would like to know what everyone in Histoland is using for the Muci Stain. Thanks! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Mon Jan 25 14:21:36 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Jan 25 14:21:52 2010 Subject: [Histonet] Mucicarmine Stains In-Reply-To: <531434241-1264450358-cardhu_decombobulator_blackberry.rim.net-1830481601-@bda112.bisx.prod.on.blackberry> References: <531434241-1264450358-cardhu_decombobulator_blackberry.rim.net-1830481601-@bda112.bisx.prod.on.blackberry> Message-ID: <4B5DB700.59CD.00EE.0@hurleymc.com> We search and tested and searched some more. Then Anatech came up with a wonderful replacement called: EZ Mucicarmine - it is ready to use and stains mucin like the good 'ol days .........30 yrs ago!! Very bright carmine staining. Hope this helped, Lynette From asmith <@t> mail.barry.edu Mon Jan 25 14:33:07 2010 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Mon Jan 25 14:35:20 2010 Subject: [Histonet] RE: Mucicarmine Stains In-Reply-To: References: Message-ID: <70AC46F12D9EF2438760A84225B6BC100509B4C5@EX2010-01.barrynet.barry.edu> I use Sheehan and Hrapchak's formula and stain 1/2 hour at 45 degrees C. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B Sent: Monday, January 25, 2010 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mucicarmine Stains We are having some issues with our Muci stain being pale. I would like to know what everyone in Histoland is using for the Muci Stain. Thanks! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Mon Jan 25 15:35:53 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Jan 25 15:36:00 2010 Subject: [Histonet] Georgia Society for Histotechnology Meeting in March Message-ID: <9BF995BC0E47744E9673A41486E24EE22429DAB57A@MERCERMAIL.MercerU.local> Hi Fellow Netters, Our program and registration form is now finalized and up on our website at www.histosearch.com/gsh. GSH invites you to Georgia where we hope soon the weather will be warmer, and to take advantage of our meeting and a chance to take in the sights, sounds and southern hospitality Georgia has to offer. The 38th annual GSH meeting will be held at the Evergreen Marriott Convention Resort, Stone Mountain, Georgia on March 26-28, 2010. Please call for reservations now at 888-670-2250. Be sure to tell them you are attending the GSH meeting. The Marriott address is 4021 Lakeview Drive, Stone Mountain, GA 30083. Visit their hotel here http://www.marriott.com/hotels/hotel-photos/ATLEG/?mktcmp=w_regionsite_atleg_x. Room rates begin at $99. Plan your family vacation now. Come to Georgia and experience the famous Southern Hospitality and all the Atlanta area attractions. Plus our meeting is two and a half weeks after the new Annual Histotechnology Professionals Day, which gives us a chance to educate communities about our contribution to their lives. Also check out what our vendors have to offer us as histology professionals. If you have questions or trouble downloading the program and registration forms from the website, let me know. Shirley A. Powell, HT(ASCP)HTL, QIHC GSH Secretary Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From jpastor1 <@t> nycap.rr.com Mon Jan 25 17:29:03 2010 From: jpastor1 <@t> nycap.rr.com (Joseph N. Pastore) Date: Mon Jan 25 17:29:12 2010 Subject: [Histonet] Histology Position Message-ID: <1AC38C0D7E894684B374D16BD728A020@DC3X31G1> Am retiring from the Albany VAMC and am looking for a supv or tech position in a Histo Lab in upstate NY or Vermont. From rsrichmond <@t> gmail.com Mon Jan 25 19:49:45 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Jan 25 19:49:50 2010 Subject: [Histonet] Re: Diff-Quik Message-ID: Thanks, John Kiernan, for your explanation of Romanovsky stains. "Diff-Quik" (please note the spelling) is the trademarked name of a staining sequence consisting of a fixative, eosin (Diff-Quik I), and an azure (Diff-Quik II), done in that order in three separate containers. I'm not sure who the trademark presently belongs to - it seems to change with the phases of the Moon. There are a number of generic equivalents, which in my personal experience all work as well as trademark Diff-Quik. For most ordinary pathology services, it isn't worthwhile to try to brew your own. I don't think I've seen bone marrow stained with such a sequence. Proper staining of bone marrows requires that the histotechnologist examine the slides under a microscope, a practice too many find abhorrent. Bob Richmond Samurai Pathoogist Knoxville TN From aevans <@t> wellspan.org Tue Jan 26 06:48:57 2010 From: aevans <@t> wellspan.org (Evans, Andria B) Date: Tue Jan 26 06:49:06 2010 Subject: [Histonet] Sticky paraffin Message-ID: We have been having some issues with sticky paraffin. We have tried all different kinds or paraffin and have come to the conclusion that it isn't the paraffin, but i'm thinking it might be another factor causing this. This does not happen everyday, but sporadic. Does anyone have any suggestions on something we can do to reduce the stickiness? Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From cmiller <@t> physlab.com Tue Jan 26 08:03:32 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Jan 26 08:03:38 2010 Subject: [Histonet] Re: Diff-Quik In-Reply-To: References: Message-ID: Every slide I stain, special stains, IHC or otherwise I check under the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, January 25, 2010 7:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Diff-Quik Thanks, John Kiernan, for your explanation of Romanovsky stains. "Diff-Quik" (please note the spelling) is the trademarked name of a staining sequence consisting of a fixative, eosin (Diff-Quik I), and an azure (Diff-Quik II), done in that order in three separate containers. I'm not sure who the trademark presently belongs to - it seems to change with the phases of the Moon. There are a number of generic equivalents, which in my personal experience all work as well as trademark Diff-Quik. For most ordinary pathology services, it isn't worthwhile to try to brew your own. I don't think I've seen bone marrow stained with such a sequence. Proper staining of bone marrows requires that the histotechnologist examine the slides under a microscope, a practice too many find abhorrent. Bob Richmond Samurai Pathoogist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From algranth <@t> email.arizona.edu Tue Jan 26 08:41:38 2010 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Tue Jan 26 08:41:46 2010 Subject: [Histonet] Sticky paraffin In-Reply-To: References: Message-ID: <72C57331-E4CD-434C-AB9E-04CF11698DBF@email.arizona.edu> Can you explain exactly what you mean by "sticky"? Where is it sticky? At embedding or cutting or both? Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Jan 26, 2010, at 5:48 AM, Evans, Andria B wrote: > We have been having some issues with sticky paraffin. We have tried > all different kinds or paraffin and have come to the conclusion that > it isn't the paraffin, but i'm thinking it might be another factor > causing this. This does not happen everyday, but sporadic. Does > anyone have any suggestions on something we can do to reduce the > stickiness? > > Andria B Evans, HTL(ASCP)CM > Anatomic Pathology > York Hospital > 1001 S. George Street > York, PA 17405 > > "You can learn a lot more from listening than you can from talking. > Find someone with whom you don't agree in the slightest and ask them > to explain themselves at length. Then take a seat, shut your mouth, > and don't argue back. It's physically impossible to listen with > your mouth open." -John Moe > > "Maturity is accepting imperfections." > > CONFIDENTIALITY NOTICE: > > This email may contain confidential health information that is > legally privileged. This information is intended for the use of the > named recipient(s). The authorized recipient of this information is > prohibited from disclosing this information to any party unless > required to do so by law or regulation and is required to destroy > the information after its stated need has been fulfilled. If you > are not the intended recipient, you are hereby notified that any > disclosure, copying, distribution, or action taken in reliance on > the contents of this email is strictly prohibited. If you receive > this e-mail message in error, please notify the sender immediately > to arrange disposition of the information. . > > > ______________________________________________________________________ > This e-mail has been scanned by MCI Managed Email Content Service, > using Skeptic(tm) technology powered by MessageLabs. For more > information on MCI's Managed Email Content Service, visit http://www.mci.com > . > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From trathborne <@t> somerset-healthcare.com Tue Jan 26 09:01:07 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jan 26 09:01:13 2010 Subject: [Histonet] H&E stainer/coverslippers In-Reply-To: <0C96F0BFE078D74C91A1C541D24A6AE49CB92CA3@EMGMB1.ad.medctr.ucla.edu> Message-ID: We have the Leica ST5020 and CV5030. Size was also a key reason for our decision. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Linke, Noelle Sent: Monday, January 25, 2010 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E stainer/coverslippers Hi all, Is anyone using a slide stainer and coverslipper combo which is NOT Sakura that they are pleased with? The new Sakura coverslipper is enormous and too big for the needs of the smaller lab we are setting up. Thank you! Noelle No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services UCLA Department of Pathology and Laboratory Medicine Phone: 310-825-7397 Fax: 310-983-3289 nlinke@mednet.ucla.edu Pager: 97471 ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From rgenest <@t> uwaterloo.ca Tue Jan 26 09:36:14 2010 From: rgenest <@t> uwaterloo.ca (Reno Genest) Date: Tue Jan 26 09:36:25 2010 Subject: [Histonet] Simple dye for cornea and sclera. Message-ID: <20100126103614.202699rzlx2eurcc@www.nexusmail.uwaterloo.ca> Hello everyone, I am trying to reconstruct the 3D geometry of the cornea and sclera of a chicken eye. I embedded and froze an eye using OCT and CO2 liquid withdrawal and sliced it using a microtome. I took pictures from the top of the eye (not the sections but what is left after slicing) using a digital camera. The problem is that the sclera and cornea are difficult to see. Is there a dye I could use before taking a picture that can be applied in one step and do not require a lengthy protocol? I cannot afford to wash the eye with tap water because it is still frozen on the microtome. Also, is anyone aware of a dye that would permeate through the sclera and cornea so that I could just dip the eye in before slicing it? Thank you. Reno Genest MSc. candidate Mechanical engineering University of Waterloo From Debra.Ortiz <@t> uchospitals.edu Tue Jan 26 09:36:21 2010 From: Debra.Ortiz <@t> uchospitals.edu (Debra.Ortiz@uchospitals.edu) Date: Tue Jan 26 09:36:43 2010 Subject: [Histonet] Bone marrow processing on Peloris Message-ID: <5392DB699B157E4C8E65B3779634BD7D018479CB@uchmbx04-hpk03s.UCHAD.uchospitals.edu> Good morning, I am currently looking at the possibility of running bone marrow cores and clots on our Peloris. Has anyone had success with this, and if so would you be willing to share your protocol? We use xylene on our runs. Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** From mwich <@t> 7thwavelabs.com Tue Jan 26 10:14:26 2010 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Tue Jan 26 10:14:31 2010 Subject: [Histonet] removing trigeminal nerve and ganglion Message-ID: <62A8156F8071C8439080D626DF8C33A6CEB398@wave-mail.7thwave.local> Does anyone have experience/a good method for removing trigeminal nerve and ganglion from rat intact? Any advice would be helpful. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From rjbuesa <@t> yahoo.com Tue Jan 26 10:20:43 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 26 10:20:51 2010 Subject: [Histonet] Sticky paraffin In-Reply-To: Message-ID: <842569.47389.qm@web65715.mail.ac4.yahoo.com> If you carry-on an excess of xylene (or whatever clearing agent you are using) into the paraffin this problem may occur. Ren? J. --- On Tue, 1/26/10, Evans, Andria B wrote: From: Evans, Andria B Subject: [Histonet] Sticky paraffin To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 26, 2010, 7:48 AM We have been having some issues with sticky paraffin.? We have tried all different kinds or paraffin and have come to the conclusion that it isn't the paraffin, but i'm thinking it might be another factor causing this.? This does not happen everyday, but sporadic.? Does anyone have any suggestions on something we can do to reduce the stickiness? Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA? 17405 "You can learn a lot more from listening than you can from talking.? Find someone with whom you don't agree in the slightest and ask them to explain themselves at length.? Then take a seat, shut your mouth, and don't argue back.? It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE:? This email may contain confidential health information that is legally privileged.? This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited.? If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email? Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 26 10:24:58 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 26 10:25:02 2010 Subject: [Histonet] Simple dye for cornea and sclera. In-Reply-To: <20100126103614.202699rzlx2eurcc@www.nexusmail.uwaterloo.ca> Message-ID: <364347.89593.qm@web65711.mail.ac4.yahoo.com> IF you can get hold of it, a phase microscope will be the best solution. You could also try to use "oblique" illumination by off-centering the condenser diaphragm. Ren? J. --- On Tue, 1/26/10, Reno Genest wrote: From: Reno Genest Subject: [Histonet] Simple dye for cornea and sclera. To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 26, 2010, 10:36 AM Hello everyone, I am trying to reconstruct the 3D geometry of the cornea and sclera of a chicken eye. I embedded and froze an eye using OCT and CO2 liquid withdrawal and sliced it using a microtome. I took pictures from the top of the eye (not the sections but what is left after slicing) using a digital camera. The problem is that the sclera and cornea are difficult to see. Is there a dye I could use before taking a picture that can be applied in one step and do not require a lengthy protocol? I cannot afford to wash the eye with tap water because it is still frozen on the microtome. Also, is anyone aware of a dye that would permeate through the sclera and cornea so that I could just dip the eye in before slicing it? Thank you. Reno Genest MSc. candidate Mechanical engineering University of Waterloo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Tue Jan 26 10:26:00 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Jan 26 10:26:06 2010 Subject: [Histonet] H&E stainer/coverslippers In-Reply-To: References: <0C96F0BFE078D74C91A1C541D24A6AE49CB92CA3@EMGMB1.ad.medctr.ucla.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F87@PHSXMB30.partners.org> As I mentioned in a previous email, we purchased refurbished Leica 5030 and Leica XL stainer, not only for price, but size as well. Happy with both. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 26, 2010 10:01 AM To: Linke, Noelle; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E stainer/coverslippers We have the Leica ST5020 and CV5030. Size was also a key reason for our decision. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Linke, Noelle Sent: Monday, January 25, 2010 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E stainer/coverslippers Hi all, Is anyone using a slide stainer and coverslipper combo which is NOT Sakura that they are pleased with? The new Sakura coverslipper is enormous and too big for the needs of the smaller lab we are setting up. Thank you! Noelle No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services UCLA Department of Pathology and Laboratory Medicine Phone: 310-825-7397 Fax: 310-983-3289 nlinke@mednet.ucla.edu Pager: 97471 ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rjbuesa <@t> yahoo.com Tue Jan 26 10:28:48 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 26 10:28:52 2010 Subject: [Histonet] Bone marrow processing on Peloris In-Reply-To: <5392DB699B157E4C8E65B3779634BD7D018479CB@uchmbx04-hpk03s.UCHAD.uchospitals.edu> Message-ID: <244951.4057.qm@web65703.mail.ac4.yahoo.com> A good tissue processor can handle those specimens without the need of any protocol modification. Ren? J. --- On Tue, 1/26/10, Debra.Ortiz@uchospitals.edu wrote: From: Debra.Ortiz@uchospitals.edu Subject: [Histonet] Bone marrow processing on Peloris To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 26, 2010, 10:36 AM Good morning, I am currently looking at the possibility of running bone marrow cores and clots on our Peloris. Has anyone had success with this, and if so would you be willing to share your protocol? We use xylene on our runs. Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kfineout <@t> hotmail.com Tue Jan 26 10:55:20 2010 From: kfineout <@t> hotmail.com (Kelly Larson) Date: Tue Jan 26 10:55:25 2010 Subject: [Histonet] How Have You Been Lately? Message-ID: Hello Dear, How are you doing these days? You know, I bought one new pair of Nike shoes on one great online shop www.HOYOY.com. They are so amazing! They provide a lot of other brand new casual shoes, sport shoes, high heels, ugg boots, such as Prada, Christian Louboutin, Gucci, Lascote, Puma, and so on. The styles are the most popular ones for the 2010 year. Have to say it is a great and specialize online shoes store. Hope you can find some great things there too www.HOYOY.com.! Give my regards to your family then From sharon.willman <@t> bms.com Tue Jan 26 11:11:18 2010 From: sharon.willman <@t> bms.com (Willman, Sharon) Date: Tue Jan 26 11:11:49 2010 Subject: [Histonet] Histology Special Stains for Macrophages Message-ID: <4E17A1A86498044BA9E35001C8B7C6038391973F@ushpwbmsmmp008.one.ads.bms.com> Hi, We are needing to do a special stain for macrophages. What is the most common stain for that? Does anyone do a Sudan Black, Alcian Blue or Van Gieson for macrophages? Any information would be appreciated. Thanks in advance. Sharon ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From sjromey <@t> bellsouth.net Tue Jan 26 11:17:04 2010 From: sjromey <@t> bellsouth.net (Shirley Romey) Date: Tue Jan 26 11:17:09 2010 Subject: Fw: [Histonet] How Have You Been Lately? Message-ID: <579719.61468.qm@web180609.mail.sp1.yahoo.com> spam is getting through ----- Forwarded Message ---- From: Kelly Larson To: histonet@lists.utsouthwestern.edu Sent: Tue, January 26, 2010 11:55:20 AM Subject: [Histonet] How Have You Been Lately? Hello Dear, How are you doing these days? You know, I bought one new pair of Nike shoes on one great online shop www.HOYOY.com. They are so amazing! They provide a lot of other brand new casual shoes, sport shoes, high heels, ugg boots, such as Prada, Christian Louboutin, Gucci, Lascote, Puma, and so on. The styles are the most popular ones for the 2010 year. Have to say it is a great and specialize online shoes store. Hope you can find some great things there too www.HOYOY.com.! Give my regards to your family then ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 26 11:47:54 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 26 11:48:04 2010 Subject: [Histonet] Histology Special Stains for Macrophages In-Reply-To: <4E17A1A86498044BA9E35001C8B7C6038391973F@ushpwbmsmmp008.one.ads.bms.com> Message-ID: <673375.69966.qm@web65714.mail.ac4.yahoo.com> Giemsa will stain them very well. Ren? J. --- On Tue, 1/26/10, Willman, Sharon wrote: From: Willman, Sharon Subject: [Histonet] Histology Special Stains for Macrophages To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, January 26, 2010, 12:11 PM Hi, We are needing to do a special stain for macrophages.? What is the most common stain for that?? Does anyone do a Sudan Black, Alcian Blue or Van Gieson for macrophages?? Any information would be appreciated. Thanks in advance. Sharon ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Tue Jan 26 13:09:51 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jan 26 13:11:28 2010 Subject: [Histonet] Coverslipping Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46DC7@nmdamailsvr.nmda.ad.nmsu.edu> Do any of my Esteemed Colleagues have a photo somewhere of someone hand-coverslipping? I need a picture of a "normal" hand coverslipping (putting coverslip ON slide instead of slide ON coverslip). Just one photo is all I need. I'm trying to describe in words how to do this and it's not easy! Thanks in advance for your Digital Donation (and I mean "electronic" digital, so don't give me any flak). If you can email it, I can pass it along. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From trathborne <@t> somerset-healthcare.com Tue Jan 26 13:15:42 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jan 26 13:15:48 2010 Subject: [Histonet] Peloris and Bond service Message-ID: We're looking into service agreements for these instruments. Can anyone who currently uses them help me decide which Leica service contract would be best? How often do you experience problems with these instruments that you need to have service for them? What is your volume? Could those service calls have been avoided if you had a/another pm done? Thank you in advance! Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From BDUE <@t> PARTNERS.ORG Tue Jan 26 17:05:40 2010 From: BDUE <@t> PARTNERS.ORG (Due, Brice) Date: Tue Jan 26 17:05:46 2010 Subject: [Histonet] 20% NaOH Nail pre-Treatment... Questions Message-ID: Hello Joe, first thanks so much for sharing your expertise here on Histonet. I work in the main histo lab at Mass General Hosp. in Boston and I have interested the powers that be in your NaOH protocol. I initially used your NaOH idea to attach an "impossible" nail to slides by floating 4um section on 20% NaOH for 10-15min. (The transfers between regular water bath and NaOH bath (and back to rinse) were surprisingly easy because the concentrated NaOH prevents adhesion to even the stickiest adhesive slides.) Anyhow, I have some questions for you about your lab's implementation. I found your most complete post at http://lists.utsouthwestern.edu/pipermail/histonet/2008-October/040078.html But I am concerned about the safety of 20% NaOH in routine use. Would you mind posting or emailing me any SOPs you have that address the safety issues? Specifically both the histology lab and the "grossing" labs here use RDO decal solutions at every bench. So there is/will be concern about the proper handling of 20% NaOH around RDO... by overworked PAs and residents, etc. etc. Feel free to tell me your safety almost-horror stories so we can (try to) prevent them from occuring here. I've seen that some people use 10% NaOH instead. Have you experimented with this? Any reasons you stick with 20% beyond the usual "it ain't broke, so..."? Have you (or anyone on Histonet) ever tried to find the minimum effective concentration? Increasing the pre-soak time isn't an issue here since I've been assured that nail turnaround times are non-critical. Along these lines, do you or anyone have any histochemical references for the reactions that are taking place? With this info it might be possible to predict the minimum effective pH. It would also be nice to have references for the SOP. (I know about the coverslip "crushes" some derm clinicians do with fresh scrapings. Alternately, do you know of any histochemical references for this?) Thank you so much for sharing your time and info! And if I happen to answer any of my own questions I will be sure to post what I learn. Sincerely, -brice Massachusetts General Hospital Surgical Pathology, Histology The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rsrichmond <@t> gmail.com Tue Jan 26 19:38:36 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Jan 26 19:38:40 2010 Subject: [Histonet] Re: Diff-Quik In-Reply-To: References: Message-ID: I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under the scope...I have taught all my techs to do the same, other than batches of ?H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. ?I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN From AnthonyH <@t> chw.edu.au Tue Jan 26 19:55:11 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jan 26 19:55:25 2010 Subject: [Histonet] Re: Diff-Quik In-Reply-To: Message-ID: Robert, I agree with your comments. There are some labs that never look at their slides (the "factories") but there are others (and this number in my experience is increasing) where the technologists often solve the problems before the slides leave the lab. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, 27 January 2010 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under > the scope...I have taught all my techs to do the same, other than > batches of ?H&E and then we check the 1st slide in each rack. I know > this to be a common procedure with many histology professionals. The > attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. ?I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jkiernan <@t> uwo.ca Tue Jan 26 23:22:53 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Jan 26 23:22:58 2010 Subject: [Histonet] Histology Special Stains for Macrophages In-Reply-To: <4E17A1A86498044BA9E35001C8B7C6038391973F@ushpwbmsmmp008.one.ads.bms.com> References: <4E17A1A86498044BA9E35001C8B7C6038391973F@ushpwbmsmmp008.one.ads.bms.com> Message-ID: None of the methods mentioned in the enquiry are stains for macrophages. Research workers who never took Histology 101 often stain cells of the monocyte/macrophage lineage immunohistochemically (IHC), using very expensive primary antibodies and fairly expensive kits to amplify and detect the binding sites. IHC is necessary if you must find every macrophage, including a tissue's recent monocyte immigrants that haven't yet done any work. Macrophages that have been busily eating blood are full of brown granules that don't need any staining. Ordinary people recognize macrophages by their appearance in sections stained with a well done H&E or with one of the many Romanowsky-Giemsa blood stains. With the latter it's important to get the pH right - much lower for sections of formaldehyde-fixed objects (pH4 to 5) than for methanol-fixed films or smears (traditionally 6.8). It's all very well explained in RD Lillie & GM Fullmer's "Histopathologic Technic..." (4th ed 1976, pp.193-197; the 3rd edition, 1965, is OK too) and also in more recent textbooks including those by Bancroft & Gamble, Geoffrey Brown, Freida Carson and, of course Yours truly. John Kiernan Anatomy, UWO London, Canada http://publish.uwo.ca/~jkiernan/bookfind.htm = = = ----- Original Message ----- From: "Willman, Sharon" Date: Tuesday, January 26, 2010 12:12 Subject: [Histonet] Histology Special Stains for Macrophages To: "histonet@lists.utsouthwestern.edu" > Hi, > We are needing to do a special stain for macrophages. What > is the most common stain for that? Does anyone do a Sudan > Black, Alcian Blue or Van Gieson for macrophages? Any > information would be appreciated. > Thanks in advance. > Sharon > > > ________________________________ > This message (including any attachments) may contain > confidential, proprietary, privileged and/or private > information. The information is intended to be for the use of > the individual or entity designated above. If you are not the > intended recipient of this message, please notify the sender > immediately, and delete the message and any attachments. Any > disclosure, reproduction, distribution or other use of this > message or any attachments by an individual or entity other than > the intended recipient is prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Jan 26 23:56:28 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Jan 26 23:56:32 2010 Subject: [Histonet] removing trigeminal nerve and ganglion In-Reply-To: <62A8156F8071C8439080D626DF8C33A6CEB398@wave-mail.7thwave.local> References: <62A8156F8071C8439080D626DF8C33A6CEB398@wave-mail.7thwave.local> Message-ID: Why don't you say who and where you are? Someone must have shown you how to take out a rat's brain. This involves cutting the roots of all the cranial nerves and the pituitary stalk. You are then looking at the base of the skull, with the overlying dura mater. The pituitary gland is in the midline, with a white centre (posterior lobe) and dark pink parts laterally (anterior lobe). There is a broad white band on each side, extending caudally from the pituitary. This comprises the trigeminal ganglion, the extradural parts of its roots and the the nerve about to branch into its three divisions. With an acutely pointed scalpel, cut down on either side and below this white object lateral and posterior to the pituitary gland. Remove and fix the excavated object. This is the biggest and easiest sensory ganglion to dissect out of a rat. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Michele Wich Date: Tuesday, January 26, 2010 11:15 Subject: [Histonet] removing trigeminal nerve and ganglion To: histonet@lists.utsouthwestern.edu > Does anyone have experience/a good method for removing > trigeminal nerve > and ganglion from rat intact? Any advice would be helpful. > From jkiernan <@t> uwo.ca Wed Jan 27 00:06:56 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Jan 27 00:06:59 2010 Subject: [Histonet] Coverslipping In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46DC7@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46DC7@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Is it "normal" to put the mounting medium on the slide and then apply a coverslip from above? What's wrong with putting the mounting medium on the supine coverslip and then bringing the slide down from above? I prefer the latter method for preparations thinner than 50um. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Breeden, Sara" Date: Tuesday, January 26, 2010 14:13 Subject: [Histonet] Coverslipping To: histonet > Do any of my Esteemed Colleagues have a photo somewhere of someone > hand-coverslipping? I need a picture of a "normal" hand > coverslipping(putting coverslip ON slide instead of slide ON > coverslip). Just one > photo is all I need. I'm trying to describe in words how > to do this and > it's not easy! Thanks in advance for your Digital Donation > (and I mean > "electronic" digital, so don't give me any flak). If you > can email it, > I can pass it along. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Wed Jan 27 07:15:54 2010 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Jan 27 07:16:21 2010 Subject: [Histonet] Coverslipping Message-ID: <8CC6D69FD7A5401-2174-11362@webmail-m099.sysops.aol.com> Before the days of automatic coverslippers, I have seen coverslipping done in large quantities from above and from below. I guess it depends what you are comfortable with. In either event, you want to get a "squeegee" effect so no air bubbles are trapped in the mountant. You need more control when coverslipping thick sections, or whole mounts. With Canada balsem, there was less problem with drying back, but that is all history now. One technologist I saw at a London hospital would coverslip and then dip the whole slide in xylene and then wipe it off . It worked surprisingly well. Mike Titford USA Pathology Mobile AL USA From sbreeden <@t> nmda.nmsu.edu Wed Jan 27 07:30:00 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jan 27 07:30:05 2010 Subject: [Histonet] Coverslipping Photo Thanks Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46DDE@nmdamailsvr.nmda.ad.nmsu.edu> Thank you to everyone that sent pictures of hand-coverslipping! I've sent them on to the tech that needed some tutoring and I believe they've been very helpful. This Histonet thing - what a bonus!! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From arvidsonkristen <@t> yahoo.com Wed Jan 27 09:06:28 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Wed Jan 27 09:06:32 2010 Subject: [Histonet] Dako vs Ventana Message-ID: <768327.23434.qm@web65709.mail.ac4.yahoo.com> Hello all, I am trying to get a feel for some of the special stainers out there.? I came to the conclusion that it will be either Dako or Ventana.? Opinions?? Any other machines on the market that people love?? Thanks. From lblazek <@t> digestivespecialists.com Wed Jan 27 09:11:48 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jan 27 09:15:58 2010 Subject: [Histonet] Dako vs Ventana In-Reply-To: <768327.23434.qm@web65709.mail.ac4.yahoo.com> References: <768327.23434.qm@web65709.mail.ac4.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390E976D90C2@IBMB7Exchange.digestivespecialists.com> I love the Intellipath from BioCare. I've had mine for over a year. It's a fully open system, it has the ability to continually add more slides to a run or start a STAT run. You can run multiplex staining. The technical support is excellent. It bench top size is a plus also. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Wednesday, January 27, 2010 10:06 AM To: histonet Subject: [Histonet] Dako vs Ventana Hello all, I am trying to get a feel for some of the special stainers out there.? I came to the conclusion that it will be either Dako or Ventana.? Opinions?? Any other machines on the market that people love?? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Jan 27 09:16:06 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Jan 27 09:16:10 2010 Subject: [Histonet] Hamamatsu Nanozoomer Message-ID: <411421.91202.qm@web50304.mail.re2.yahoo.com> Hi?Everyone, I was wondering if any of you folks had?experience with the?Hamamatsu Nanozoomer slide scanner.? We recently learned?about it and are considering getting one instead of an?Aperio slide scanner.? We plan to use Visiomorph image analysis software, so all we really need is the scanner and not all of the fancy software that these companies sell to go with their instruments. The other labs at my company have Aperio systems and the images from the Nanozoomer would need?to compatible or made to be compatabile for this to be a viable option for us (for cross-site slide conferencing purposes).? We are interested in it because this instrument can be adapted for fluorescence (with Aperio, you need to purchase 2 separate instruments; one for light microscope and one for fluorescence). Any insights would be greatly appreciated. Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From marktarango <@t> gmail.com Wed Jan 27 09:18:44 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jan 27 09:18:49 2010 Subject: [Histonet] Dako vs Ventana In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390E976D90C2@IBMB7Exchange.digestivespecialists.com> References: <768327.23434.qm@web65709.mail.ac4.yahoo.com> <5A2BD13465E061429D6455C8D6B40E390E976D90C2@IBMB7Exchange.digestivespecialists.com> Message-ID: <5b6eb13e1001270718k712a5987ned3c92c31c443092@mail.gmail.com> Are you really running special stains on the Intellipath? Mark Tarango On Wed, Jan 27, 2010 at 7:11 AM, Blazek, Linda < lblazek@digestivespecialists.com> wrote: > I love the Intellipath from BioCare. I've had mine for over a year. It's a > fully open system, it has the ability to continually add more slides to a > run or start a STAT run. You can run multiplex staining. The technical > support is excellent. It bench top size is a plus also. > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson > Sent: Wednesday, January 27, 2010 10:06 AM > To: histonet > Subject: [Histonet] Dako vs Ventana > > Hello all, > I am trying to get a feel for some of the special stainers out there. I > came to the conclusion that it will be either Dako or Ventana. Opinions? > Any other machines on the market that people love? Thanks. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kmerriam2003 <@t> yahoo.com Wed Jan 27 09:23:12 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Jan 27 09:23:15 2010 Subject: [Histonet] Dianova rat anti-mouse CD31 Message-ID: <990075.43781.qm@web50308.mail.re2.yahoo.com> Hi All, I know there was some buzz a while back about this antibody.? I have tested it on mouse FFPE xenografts, and I must say, it looks very promising.? I compared 2?pieces of?the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was?fixed in NBF.? The NBF-fixed tumor actually looks better! A couple of other folks that are working at?the west coast sites of my company have had similar results.? We are all very encouraged. I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From lhadley <@t> iupui.edu Wed Jan 27 09:28:34 2010 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Wed Jan 27 09:28:39 2010 Subject: [Histonet] RE: [IHCRG] Dianova rat anti-mouse CD31 In-Reply-To: <990075.43781.qm@web50308.mail.re2.yahoo.com> References: <990075.43781.qm@web50308.mail.re2.yahoo.com> Message-ID: <5E6A94F8037F4F49B738F5B6AD16952215EC2BEC2D@iu-mssg-mbx09.ads.iu.edu> We are having good results also. Lee Ann Baldridge IUSM Indpls., IN. ________________________________ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Kim Merriam Sent: Wednesday, January 27, 2010 10:23 AM To: Histonet; ihcrg@googlegroups.com Subject: [IHCRG] Dianova rat anti-mouse CD31 Hi All, I know there was some buzz a while back about this antibody. I have tested it on mouse FFPE xenografts, and I must say, it looks very promising. I compared 2 pieces of the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was fixed in NBF. The NBF-fixed tumor actually looks better! A couple of other folks that are working at the west coast sites of my company have had similar results. We are all very encouraged. I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From Kim.Donadio <@t> bhcpns.org Wed Jan 27 09:52:58 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Jan 27 09:53:26 2010 Subject: [Histonet] Re: Diff-Quik In-Reply-To: Message-ID: I'm going to have to agree with Cheryl on the comment. This may be your experience but I can tell you my techs always look at their stains before they send it on to the Pathologist. It is a requirement that they understand what they are looking at in order to know if it worked. Each of them are also trained to know all tissues microscopically and all stain components microscopically. That is after all the purpose of being a Histologist. I am going out on a limb here and I normally don't, but you are digging yourself in to a rather rude hole to insult so many professional Histologist. Just saying............. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 01/26/2010 07:38 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From mpence <@t> grhs.net Wed Jan 27 10:09:20 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jan 27 10:09:27 2010 Subject: [Histonet] Re: Diff-Quik In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D20@is-e2k3.grhs.net> Give it a rest! Dr. Richmond 'sort of apologized' for his comments. He does explain his views and why he stated them. That should be the end of it! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, January 27, 2010 9:53 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Diff-Quik I'm going to have to agree with Cheryl on the comment. This may be your experience but I can tell you my techs always look at their stains before they send it on to the Pathologist. It is a requirement that they understand what they are looking at in order to know if it worked. Each of them are also trained to know all tissues microscopically and all stain components microscopically. That is after all the purpose of being a Histologist. I am going out on a limb here and I normally don't, but you are digging yourself in to a rather rude hole to insult so many professional Histologist. Just saying............. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 01/26/2010 07:38 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under > the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Wed Jan 27 10:11:00 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Jan 27 10:11:47 2010 Subject: [Histonet] Dako vs Ventana In-Reply-To: <768327.23434.qm@web65709.mail.ac4.yahoo.com> References: <768327.23434.qm@web65709.mail.ac4.yahoo.com> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A5621@EXCHMBC2.ad.ah.local> We find the Ventana very easy and it takes a very short time to load/set up. We did demo the DAKO and my techs felt it was too easy to make errors and took too long to get a run going. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Wednesday, January 27, 2010 9:06 AM To: histonet Subject: [Histonet] Dako vs Ventana Hello all, I am trying to get a feel for some of the special stainers out there. I came to the conclusion that it will be either Dako or Ventana. Opinions? Any other machines on the market that people love? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Kim.Donadio <@t> bhcpns.org Wed Jan 27 10:17:18 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Jan 27 10:17:39 2010 Subject: [Histonet] Re: Diff-Quik In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3D20@is-e2k3.grhs.net> Message-ID: Thanks for your profound attitude. A "sort of apology" is like a boat that "sort of floats" . I am surprised at the unprofessional comments that get sent to my desk from here every day. Not sure if it is really worth it. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Mike Pence" 01/27/2010 10:09 AM To , "Robert Richmond" cc , Subject RE: [Histonet] Re: Diff-Quik Give it a rest! Dr. Richmond 'sort of apologized' for his comments. He does explain his views and why he stated them. That should be the end of it! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, January 27, 2010 9:53 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Diff-Quik I'm going to have to agree with Cheryl on the comment. This may be your experience but I can tell you my techs always look at their stains before they send it on to the Pathologist. It is a requirement that they understand what they are looking at in order to know if it worked. Each of them are also trained to know all tissues microscopically and all stain components microscopically. That is after all the purpose of being a Histologist. I am going out on a limb here and I normally don't, but you are digging yourself in to a rather rude hole to insult so many professional Histologist. Just saying............. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 01/26/2010 07:38 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under > the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cory.collins <@t> DHAT.com Wed Jan 27 10:25:16 2010 From: cory.collins <@t> DHAT.com (Cory Collins) Date: Wed Jan 27 10:25:23 2010 Subject: [Histonet] Pathology Laboratory Manager Opening in Houston, Texas Message-ID: SightLine is expanding their service lines and is seeking a pathology laboratory manager who will be responsible for the pathology laboratory services from the design and construction of the new facility, equipment purchasing, hiring staff, policies and procedures, certification and licensure. The ideal candidate must have managed laboratory staff and have been through CLIA certification. They must also have the ability to step-in as a laboratory technician should the situation demanded it. The new facility will be located on South Main near the Reliant Stadium in Houston, Texas Reports to Vice President of Operations. Education: Bachelor Degree in Science with appropriate on the job training. Please indicate your interest by sending emails with resumes attached to hr@sightlinehealth.com From rfields <@t> gidocs.net Wed Jan 27 10:24:54 2010 From: rfields <@t> gidocs.net (Rosa Fields) Date: Wed Jan 27 10:26:23 2010 Subject: [Histonet] Dako vs Ventana References: <768327.23434.qm@web65709.mail.ac4.yahoo.com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A5621@EXCHMBC2.ad.ah.local> Message-ID: <07732CE52EC3174AB891DE1C62DB4D8FC7B68F@GIEXCHANGE.gidocs.net> I agree with Jan, I just got a Ventana for Christmas (thanks Santa).. ok, just a coincidence of timing, but I really like it. It is very simple to set up and walk away from. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, January 27, 2010 10:11 AM To: 'kristen arvidson'; histonet Subject: RE: [Histonet] Dako vs Ventana We find the Ventana very easy and it takes a very short time to load/set up. We did demo the DAKO and my techs felt it was too easy to make errors and took too long to get a run going. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Wednesday, January 27, 2010 9:06 AM To: histonet Subject: [Histonet] Dako vs Ventana Hello all, I am trying to get a feel for some of the special stainers out there. I came to the conclusion that it will be either Dako or Ventana. Opinions? Any other machines on the market that people love? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Wed Jan 27 10:35:31 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Jan 27 10:36:06 2010 Subject: [Histonet] Re: Diff-Quik In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3D20@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3D20@is-e2k3.grhs.net> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A5622@EXCHMBC2.ad.ah.local> I find this an interesting subject. I have worked with many techs over my 30+ year career and have found that about half the techs could care less about looking at slides at the scope. When I first came to my present facility and put a scope on the special stain counter some of the techs thought I was "full of myself" acting like a Pathologist. I would never suppose to compare myself with a Pathologist but I'm personally not going to turn in a special stain that I am not sure worked properly. I think the major problem is that those techs that don't care to "look", don't know what they are looking at. Dr. Richmond is correct, most Pathologists will not take the time to teach techs. That is why we have to teach each other and make good use of the Pathologist who are willing. Workshops at the NSH that deal with tx id and special stains are usually packed full. There is a real need out there for this basic training. Like others, we look at and document that our controls worked before sending slides to the Pathologists. It was a struggle with a few people who are still not sure of themselves but they keep trying. We, as professionals, need to keep teaching our own. Support your National and local societies, and if you know something, mentor somebody who needs the help. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 27, 2010 10:09 AM To: Kim.Donadio@bhcpns.org; Robert Richmond Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Diff-Quik Give it a rest! Dr. Richmond 'sort of apologized' for his comments. He does explain his views and why he stated them. That should be the end of it! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, January 27, 2010 9:53 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Diff-Quik I'm going to have to agree with Cheryl on the comment. This may be your experience but I can tell you my techs always look at their stains before they send it on to the Pathologist. It is a requirement that they understand what they are looking at in order to know if it worked. Each of them are also trained to know all tissues microscopically and all stain components microscopically. That is after all the purpose of being a Histologist. I am going out on a limb here and I normally don't, but you are digging yourself in to a rather rude hole to insult so many professional Histologist. Just saying............. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 01/26/2010 07:38 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under > the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Kim.Donadio <@t> bhcpns.org Wed Jan 27 10:51:55 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Jan 27 10:52:24 2010 Subject: [Histonet] Re: Diff-Quik In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A5622@EXCHMBC2.ad.ah.local> Message-ID: All of my techs( including myself ) and just about every tech I know in Florida went to college and took Histology and Histopathology from a (NAACLS) approved program. I guess it depends on where you live. Here and for most of this state ( from what I have seen ) for the techs to look at their work is the norm, it is expected. Different areas, diffrent expectations, no need for rudness or to belittle, lessons learned. Hope all have a great week! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Mahoney,Janice A" 01/27/2010 10:35 AM To 'Mike Pence' , "Kim.Donadio@bhcpns.org" , Robert Richmond cc "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Subject RE: [Histonet] Re: Diff-Quik I find this an interesting subject. I have worked with many techs over my 30+ year career and have found that about half the techs could care less about looking at slides at the scope. When I first came to my present facility and put a scope on the special stain counter some of the techs thought I was "full of myself" acting like a Pathologist. I would never suppose to compare myself with a Pathologist but I'm personally not going to turn in a special stain that I am not sure worked properly. I think the major problem is that those techs that don't care to "look", don't know what they are looking at. Dr. Richmond is correct, most Pathologists will not take the time to teach techs. That is why we have to teach each other and make good use of the Pathologist who are willing. Workshops at the NSH that deal with tx id and special stains are usually packed full. There is a real need out there for this basic training. Like others, we look at and document that our controls worked before sending slides to the Pathologists. It was a struggle with a few people who are still not sure of themselves but they keep trying. We, as professionals, need to keep teaching our own. Support your National and local societies, and if you know something, mentor somebody who needs the help. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 27, 2010 10:09 AM To: Kim.Donadio@bhcpns.org; Robert Richmond Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Diff-Quik Give it a rest! Dr. Richmond 'sort of apologized' for his comments. He does explain his views and why he stated them. That should be the end of it! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, January 27, 2010 9:53 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Diff-Quik I'm going to have to agree with Cheryl on the comment. This may be your experience but I can tell you my techs always look at their stains before they send it on to the Pathologist. It is a requirement that they understand what they are looking at in order to know if it worked. Each of them are also trained to know all tissues microscopically and all stain components microscopically. That is after all the purpose of being a Histologist. I am going out on a limb here and I normally don't, but you are digging yourself in to a rather rude hole to insult so many professional Histologist. Just saying............. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 01/26/2010 07:38 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under > the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From liz <@t> premierlab.com Wed Jan 27 11:08:55 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jan 27 11:09:00 2010 Subject: [Histonet] RE: [IHCRG] Hamamatsu Nanozoomer References: <411421.91202.qm@web50304.mail.re2.yahoo.com> Message-ID: Kim We have an Aperio Scanner. I'm not quite sure what you are asking but this is what I think from your e-mail. Aperio scans create .svs files these files are considered a modified tiff file. When aperio scans the file is placed into the spectrum database - from there you can create case or project files, etc. These images can be viewed via imagescope. There is no issue with placing other images that have not been generated on the scanscope into the spectrum database, you can upload any image into the spectrum database we have done this in the past. These images can also be viewed with imagescope we have only uploaded single magnification tiff files into the aperio spectrum database. The Hamamatsu Nanozoomer I believe also comes with a database and some viewing sofware. Are you asking if you can view the HN images in the spectrum database? To be honest I'm not sure if you can view the images completed with image scope I would ask your sales rep. I'm sure you are aware that both of these scanners essentially create virtual slides. Not just an image of a slide at one magnification. The viewing software allows you to view the images at different magnifications. I'm not that familar with Visiomorph its the Olympus product and doesn't Olympus sell the HN scanner? I would also think that that the database that comes with the HN scanner should be able to accept other images and should have some conferencing capabilities, I know that the aperio does. I'm not sure if I answered your questions, but I would ask the sales reps from both aperio and Olympus about compatability. With repects to analysis I think that the Visiomorph software will be able to analyze a image generated from the scanscope. If you are working with an HN scanner and the visiomorph software I'm assuming that you will be able to perform batch analysis on selected images from the olympus database. You have to find out if you can run batch analysis out of either of the databases with the Visiomorph software - if you have lots of slides to analyze then setting them up one at a time is too labor intensive. You can call me if you want to, I'm at a conference but will be back in the lab tomorrow (303) 682-3949 Liz ________________________________ From: ihcrg@googlegroups.com on behalf of Kim Merriam Sent: Wed 1/27/2010 8:16 AM To: Histonet; ihcrg@googlegroups.com Subject: [IHCRG] Hamamatsu Nanozoomer Hi Everyone, I was wondering if any of you folks had experience with the Hamamatsu Nanozoomer slide scanner. We recently learned about it and are considering getting one instead of an Aperio slide scanner. We plan to use Visiomorph image analysis software, so all we really need is the scanner and not all of the fancy software that these companies sell to go with their instruments. The other labs at my company have Aperio systems and the images from the Nanozoomer would need to compatible or made to be compatabile for this to be a viable option for us (for cross-site slide conferencing purposes). We are interested in it because this instrument can be adapted for fluorescence (with Aperio, you need to purchase 2 separate instruments; one for light microscope and one for fluorescence). Any insights would be greatly appreciated. Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From Theresa.Oeler <@t> nsabp.org Wed Jan 27 11:49:42 2010 From: Theresa.Oeler <@t> nsabp.org (Oeler, Theresa) Date: Wed Jan 27 11:49:59 2010 Subject: [Histonet] RE: Histonet Digest, Vol 74, Issue 32 In-Reply-To: <20100127164614.7FFDEBAE5E@Draconis.nsabp.org> References: <20100127164614.7FFDEBAE5E@Draconis.nsabp.org> Message-ID: <3143A79C6CDE654183334C7FD94C1B7D02E391A7@mercury.NSABP.org> Hi all Have you looked into the BioImagene Concerto scanner? This scanner can provide dark feld and brightfield image analysis in addition to the telepathology, frozen section collaboration capability, education archival, and the works. This scanner, from what I hear is already available or will be available in March. The rate of scan, the image analysis and software package (virtuoso software package) is quite amazing! And there is NOTHING else in the marketplace Check out the website: bioimagene.com For more details T Oeler No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. Theresa A Oeler, BS Senior Research Histologist NSABP Pathology Laboratory Federal North Building 1307 Federal Street, Suite 303 Pittsburgh, PA 15212 412/359-8931 Of. 412/359-3239 Fx. theresa.oeler@nsabp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, January 27, 2010 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 74, Issue 32 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Coverslipping (Breeden, Sara) 2. Peloris and Bond service (Rathborne, Toni) 3. 20% NaOH Nail pre-Treatment... Questions (Due, Brice) 4. Re: Diff-Quik (Robert Richmond) 5. RE: Re: Diff-Quik (Tony Henwood) 6. Re: Histology Special Stains for Macrophages (John Kiernan) 7. Re: removing trigeminal nerve and ganglion (John Kiernan) 8. Re: Coverslipping (John Kiernan) 9. Coverslipping (mtitford@aol.com) 10. Coverslipping Photo Thanks (Breeden, Sara) 11. Dako vs Ventana (kristen arvidson) 12. RE: Dako vs Ventana (Blazek, Linda) 13. Hamamatsu Nanozoomer (Kim Merriam) 14. Re: Dako vs Ventana (Mark Tarango) 15. Dianova rat anti-mouse CD31 (Kim Merriam) 16. RE: [IHCRG] Dianova rat anti-mouse CD31 (Baldridge, Lee Ann) 17. Re: Re: Diff-Quik (Kim.Donadio@bhcpns.org) 18. RE: Re: Diff-Quik (Mike Pence) 19. RE: Dako vs Ventana (Mahoney,Janice A) 20. RE: Re: Diff-Quik (Kim.Donadio@bhcpns.org) 21. Pathology Laboratory Manager Opening in Houston, Texas (Cory Collins) ---------------------------------------------------------------------- Message: 1 Date: Tue, 26 Jan 2010 12:09:51 -0700 From: "Breeden, Sara" Subject: [Histonet] Coverslipping To: "histonet" Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46DC7@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Do any of my Esteemed Colleagues have a photo somewhere of someone hand-coverslipping? I need a picture of a "normal" hand coverslipping (putting coverslip ON slide instead of slide ON coverslip). Just one photo is all I need. I'm trying to describe in words how to do this and it's not easy! Thanks in advance for your Digital Donation (and I mean "electronic" digital, so don't give me any flak). If you can email it, I can pass it along. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 2 Date: Tue, 26 Jan 2010 14:15:42 -0500 From: "Rathborne, Toni" Subject: [Histonet] Peloris and Bond service To: Message-ID: Content-Type: text/plain; charset="utf-8" We're looking into service agreements for these instruments. Can anyone who currently uses them help me decide which Leica service contract would be best? How often do you experience problems with these instruments that you need to have service for them? What is your volume? Could those service calls have been avoided if you had a/another pm done? Thank you in advance! Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 3 Date: Tue, 26 Jan 2010 18:05:40 -0500 From: "Due, Brice" Subject: [Histonet] 20% NaOH Nail pre-Treatment... Questions To: "Joe \"The Toe\" Nocito" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Joe, first thanks so much for sharing your expertise here on Histonet. I work in the main histo lab at Mass General Hosp. in Boston and I have interested the powers that be in your NaOH protocol. I initially used your NaOH idea to attach an "impossible" nail to slides by floating 4um section on 20% NaOH for 10-15min. (The transfers between regular water bath and NaOH bath (and back to rinse) were surprisingly easy because the concentrated NaOH prevents adhesion to even the stickiest adhesive slides.) Anyhow, I have some questions for you about your lab's implementation. I found your most complete post at http://lists.utsouthwestern.edu/pipermail/histonet/2008-October/040078.html But I am concerned about the safety of 20% NaOH in routine use. Would you mind posting or emailing me any SOPs you have that address the safety issues? Specifically both the histology lab and the "grossing" labs here use RDO decal solutions at every bench. So there is/will be concern about the proper handling of 20% NaOH around RDO... by overworked PAs and residents, etc. etc. Feel free to tell me your safety almost-horror stories so we can (try to) prevent them from occuring here. I've seen that some people use 10% NaOH instead. Have you experimented with this? Any reasons you stick with 20% beyond the usual "it ain't broke, so..."? Have you (or anyone on Histonet) ever tried to find the minimum effective concentration? Increasing the pre-soak time isn't an issue here since I've been assured that nail turnaround times are non-critical. Along these lines, do you or anyone have any histochemical references for the reactions that are taking place? With this info it might be possible to predict the minimum effective pH. It would also be nice to have references for the SOP. (I know about the coverslip "crushes" some derm clinicians do with fresh scrapings. Alternately, do you know of any histochemical references for this?) Thank you so much for sharing your time and info! And if I happen to answer any of my own questions I will be sure to post what I learn. Sincerely, -brice Massachusetts General Hospital Surgical Pathology, Histology The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 4 Date: Tue, 26 Jan 2010 20:38:36 -0500 From: Robert Richmond Subject: [Histonet] Re: Diff-Quik To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under the scope...I have taught all my techs to do the same, other than batches of ?H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. ?I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN ------------------------------ Message: 5 Date: Wed, 27 Jan 2010 12:55:11 +1100 From: "Tony Henwood" Subject: RE: [Histonet] Re: Diff-Quik To: "Robert Richmond" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Robert, I agree with your comments. There are some labs that never look at their slides (the "factories") but there are others (and this number in my experience is increasing) where the technologists often solve the problems before the slides leave the lab. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, 27 January 2010 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under > the scope...I have taught all my techs to do the same, other than > batches of ?H&E and then we check the 1st slide in each rack. I know > this to be a common procedure with many histology professionals. The > attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. ?I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 6 Date: Wed, 27 Jan 2010 00:22:53 -0500 From: John Kiernan Subject: Re: [Histonet] Histology Special Stains for Macrophages To: "Willman, Sharon" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; CHARSET=US-ASCII None of the methods mentioned in the enquiry are stains for macrophages. Research workers who never took Histology 101 often stain cells of the monocyte/macrophage lineage immunohistochemically (IHC), using very expensive primary antibodies and fairly expensive kits to amplify and detect the binding sites. IHC is necessary if you must find every macrophage, including a tissue's recent monocyte immigrants that haven't yet done any work. Macrophages that have been busily eating blood are full of brown granules that don't need any staining. Ordinary people recognize macrophages by their appearance in sections stained with a well done H&E or with one of the many Romanowsky-Giemsa blood stains. With the latter it's important to get the pH right - much lower for sections of formaldehyde-fixed objects (pH4 to 5) than for methanol-fixed films or smears (traditionally 6.8). It's all very well explained in RD Lillie & GM Fullmer's "Histopathologic Technic..." (4th ed 1976, pp.193-197; the 3rd edition, 1965, is OK too) and also in more recent textbooks including those by Bancroft & Gamble, Geoffrey Brown, Freida Carson and, of course Yours truly. John Kiernan Anatomy, UWO London, Canada http://publish.uwo.ca/~jkiernan/bookfind.htm = = = ----- Original Message ----- From: "Willman, Sharon" Date: Tuesday, January 26, 2010 12:12 Subject: [Histonet] Histology Special Stains for Macrophages To: "histonet@lists.utsouthwestern.edu" > Hi, > We are needing to do a special stain for macrophages. What > is the most common stain for that? Does anyone do a Sudan > Black, Alcian Blue or Van Gieson for macrophages? Any > information would be appreciated. > Thanks in advance. > Sharon > > > ________________________________ > This message (including any attachments) may contain > confidential, proprietary, privileged and/or private > information. The information is intended to be for the use of > the individual or entity designated above. If you are not the > intended recipient of this message, please notify the sender > immediately, and delete the message and any attachments. Any > disclosure, reproduction, distribution or other use of this > message or any attachments by an individual or entity other than > the intended recipient is prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 27 Jan 2010 00:56:28 -0500 From: John Kiernan Subject: Re: [Histonet] removing trigeminal nerve and ganglion To: Michele Wich Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; CHARSET=US-ASCII Why don't you say who and where you are? Someone must have shown you how to take out a rat's brain. This involves cutting the roots of all the cranial nerves and the pituitary stalk. You are then looking at the base of the skull, with the overlying dura mater. The pituitary gland is in the midline, with a white centre (posterior lobe) and dark pink parts laterally (anterior lobe). There is a broad white band on each side, extending caudally from the pituitary. This comprises the trigeminal ganglion, the extradural parts of its roots and the the nerve about to branch into its three divisions. With an acutely pointed scalpel, cut down on either side and below this white object lateral and posterior to the pituitary gland. Remove and fix the excavated object. This is the biggest and easiest sensory ganglion to dissect out of a rat. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Michele Wich Date: Tuesday, January 26, 2010 11:15 Subject: [Histonet] removing trigeminal nerve and ganglion To: histonet@lists.utsouthwestern.edu > Does anyone have experience/a good method for removing > trigeminal nerve > and ganglion from rat intact? Any advice would be helpful. > ------------------------------ Message: 8 Date: Wed, 27 Jan 2010 01:06:56 -0500 From: John Kiernan Subject: Re: [Histonet] Coverslipping To: "Breeden, Sara" Cc: histonet Message-ID: Content-Type: text/plain; CHARSET=US-ASCII Is it "normal" to put the mounting medium on the slide and then apply a coverslip from above? What's wrong with putting the mounting medium on the supine coverslip and then bringing the slide down from above? I prefer the latter method for preparations thinner than 50um. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Breeden, Sara" Date: Tuesday, January 26, 2010 14:13 Subject: [Histonet] Coverslipping To: histonet > Do any of my Esteemed Colleagues have a photo somewhere of someone > hand-coverslipping? I need a picture of a "normal" hand > coverslipping(putting coverslip ON slide instead of slide ON > coverslip). Just one > photo is all I need. I'm trying to describe in words how > to do this and > it's not easy! Thanks in advance for your Digital Donation > (and I mean > "electronic" digital, so don't give me any flak). If you > can email it, > I can pass it along. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 27 Jan 2010 08:15:54 -0500 From: mtitford@aol.com Subject: [Histonet] Coverslipping To: Histonet@pathology.swmed.edu Message-ID: <8CC6D69FD7A5401-2174-11362@webmail-m099.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Before the days of automatic coverslippers, I have seen coverslipping done in large quantities from above and from below. I guess it depends what you are comfortable with. In either event, you want to get a "squeegee" effect so no air bubbles are trapped in the mountant. You need more control when coverslipping thick sections, or whole mounts. With Canada balsem, there was less problem with drying back, but that is all history now. One technologist I saw at a London hospital would coverslip and then dip the whole slide in xylene and then wipe it off . It worked surprisingly well. Mike Titford USA Pathology Mobile AL USA ------------------------------ Message: 10 Date: Wed, 27 Jan 2010 06:30:00 -0700 From: "Breeden, Sara" Subject: [Histonet] Coverslipping Photo Thanks To: "histonet" Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46DDE@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Thank you to everyone that sent pictures of hand-coverslipping! I've sent them on to the tech that needed some tutoring and I believe they've been very helpful. This Histonet thing - what a bonus!! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 11 Date: Wed, 27 Jan 2010 07:06:28 -0800 (PST) From: kristen arvidson Subject: [Histonet] Dako vs Ventana To: histonet Message-ID: <768327.23434.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello all, I am trying to get a feel for some of the special stainers out there.? I came to the conclusion that it will be either Dako or Ventana.? Opinions?? Any other machines on the market that people love?? Thanks. ------------------------------ Message: 12 Date: Wed, 27 Jan 2010 10:11:48 -0500 From: "Blazek, Linda" Subject: RE: [Histonet] Dako vs Ventana To: 'kristen arvidson' , histonet Message-ID: <5A2BD13465E061429D6455C8D6B40E390E976D90C2@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="iso-8859-1" I love the Intellipath from BioCare. I've had mine for over a year. It's a fully open system, it has the ability to continually add more slides to a run or start a STAT run. You can run multiplex staining. The technical support is excellent. It bench top size is a plus also. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Wednesday, January 27, 2010 10:06 AM To: histonet Subject: [Histonet] Dako vs Ventana Hello all, I am trying to get a feel for some of the special stainers out there.? I came to the conclusion that it will be either Dako or Ventana.? Opinions?? Any other machines on the market that people love?? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 27 Jan 2010 07:16:06 -0800 (PST) From: Kim Merriam Subject: [Histonet] Hamamatsu Nanozoomer To: Histonet , ihcrg@googlegroups.com Message-ID: <411421.91202.qm@web50304.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi?Everyone, I was wondering if any of you folks had?experience with the?Hamamatsu Nanozoomer slide scanner.? We recently learned?about it and are considering getting one instead of an?Aperio slide scanner.? We plan to use Visiomorph image analysis software, so all we really need is the scanner and not all of the fancy software that these companies sell to go with their instruments. The other labs at my company have Aperio systems and the images from the Nanozoomer would need?to compatible or made to be compatabile for this to be a viable option for us (for cross-site slide conferencing purposes).? We are interested in it because this instrument can be adapted for fluorescence (with Aperio, you need to purchase 2 separate instruments; one for light microscope and one for fluorescence). Any insights would be greatly appreciated. Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ------------------------------ Message: 14 Date: Wed, 27 Jan 2010 07:18:44 -0800 From: Mark Tarango Subject: Re: [Histonet] Dako vs Ventana To: "Blazek, Linda" Cc: histonet Message-ID: <5b6eb13e1001270718k712a5987ned3c92c31c443092@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Are you really running special stains on the Intellipath? Mark Tarango On Wed, Jan 27, 2010 at 7:11 AM, Blazek, Linda < lblazek@digestivespecialists.com> wrote: > I love the Intellipath from BioCare. I've had mine for over a year. It's a > fully open system, it has the ability to continually add more slides to a > run or start a STAT run. You can run multiplex staining. The technical > support is excellent. It bench top size is a plus also. > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson > Sent: Wednesday, January 27, 2010 10:06 AM > To: histonet > Subject: [Histonet] Dako vs Ventana > > Hello all, > I am trying to get a feel for some of the special stainers out there. I > came to the conclusion that it will be either Dako or Ventana. Opinions? > Any other machines on the market that people love? Thanks. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 15 Date: Wed, 27 Jan 2010 07:23:12 -0800 (PST) From: Kim Merriam Subject: [Histonet] Dianova rat anti-mouse CD31 To: Histonet , ihcrg@googlegroups.com Message-ID: <990075.43781.qm@web50308.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All, I know there was some buzz a while back about this antibody.? I have tested it on mouse FFPE xenografts, and I must say, it looks very promising.? I compared 2?pieces of?the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was?fixed in NBF.? The NBF-fixed tumor actually looks better! A couple of other folks that are working at?the west coast sites of my company have had similar results.? We are all very encouraged. I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ------------------------------ Message: 16 Date: Wed, 27 Jan 2010 10:28:34 -0500 From: "Baldridge, Lee Ann" Subject: [Histonet] RE: [IHCRG] Dianova rat anti-mouse CD31 To: "kmerriam2003@yahoo.com" , Histonet , "ihcrg@googlegroups.com" Message-ID: <5E6A94F8037F4F49B738F5B6AD16952215EC2BEC2D@iu-mssg-mbx09.ads.iu.edu> Content-Type: text/plain; charset="us-ascii" We are having good results also. Lee Ann Baldridge IUSM Indpls., IN. ________________________________ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Kim Merriam Sent: Wednesday, January 27, 2010 10:23 AM To: Histonet; ihcrg@googlegroups.com Subject: [IHCRG] Dianova rat anti-mouse CD31 Hi All, I know there was some buzz a while back about this antibody. I have tested it on mouse FFPE xenografts, and I must say, it looks very promising. I compared 2 pieces of the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was fixed in NBF. The NBF-fixed tumor actually looks better! A couple of other folks that are working at the west coast sites of my company have had similar results. We are all very encouraged. I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ------------------------------ Message: 17 Date: Wed, 27 Jan 2010 09:52:58 -0600 From: Kim.Donadio@bhcpns.org Subject: Re: [Histonet] Re: Diff-Quik To: Robert Richmond Cc: "histonet@lists.utsouthwestern.edu" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I'm going to have to agree with Cheryl on the comment. This may be your experience but I can tell you my techs always look at their stains before they send it on to the Pathologist. It is a requirement that they understand what they are looking at in order to know if it worked. Each of them are also trained to know all tissues microscopically and all stain components microscopically. That is after all the purpose of being a Histologist. I am going out on a limb here and I normally don't, but you are digging yourself in to a rather rude hole to insult so many professional Histologist. Just saying............. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 01/26/2010 07:38 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 18 Date: Wed, 27 Jan 2010 10:09:20 -0600 From: "Mike Pence" Subject: RE: [Histonet] Re: Diff-Quik To: , "Robert Richmond" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D20@is-e2k3.grhs.net> Content-Type: text/plain; charset="us-ascii" Give it a rest! Dr. Richmond 'sort of apologized' for his comments. He does explain his views and why he stated them. That should be the end of it! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, January 27, 2010 9:53 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Diff-Quik I'm going to have to agree with Cheryl on the comment. This may be your experience but I can tell you my techs always look at their stains before they send it on to the Pathologist. It is a requirement that they understand what they are looking at in order to know if it worked. Each of them are also trained to know all tissues microscopically and all stain components microscopically. That is after all the purpose of being a Histologist. I am going out on a limb here and I normally don't, but you are digging yourself in to a rather rude hole to insult so many professional Histologist. Just saying............. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 01/26/2010 07:38 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under > the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 27 Jan 2010 10:11:00 -0600 From: "Mahoney,Janice A" Subject: RE: [Histonet] Dako vs Ventana To: 'kristen arvidson' , histonet Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A5621@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="iso-8859-1" We find the Ventana very easy and it takes a very short time to load/set up. We did demo the DAKO and my techs felt it was too easy to make errors and took too long to get a run going. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Wednesday, January 27, 2010 9:06 AM To: histonet Subject: [Histonet] Dako vs Ventana Hello all, I am trying to get a feel for some of the special stainers out there. I came to the conclusion that it will be either Dako or Ventana. Opinions? Any other machines on the market that people love? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. 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Thank you for your cooperation. ------------------------------ Message: 20 Date: Wed, 27 Jan 2010 10:17:18 -0600 From: Kim.Donadio@bhcpns.org Subject: RE: [Histonet] Re: Diff-Quik To: "Mike Pence" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu, Robert Richmond Message-ID: Content-Type: text/plain; charset="US-ASCII" Thanks for your profound attitude. A "sort of apology" is like a boat that "sort of floats" . I am surprised at the unprofessional comments that get sent to my desk from here every day. Not sure if it is really worth it. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Mike Pence" 01/27/2010 10:09 AM To , "Robert Richmond" cc , Subject RE: [Histonet] Re: Diff-Quik Give it a rest! Dr. Richmond 'sort of apologized' for his comments. He does explain his views and why he stated them. That should be the end of it! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, January 27, 2010 9:53 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Diff-Quik I'm going to have to agree with Cheryl on the comment. This may be your experience but I can tell you my techs always look at their stains before they send it on to the Pathologist. It is a requirement that they understand what they are looking at in order to know if it worked. Each of them are also trained to know all tissues microscopically and all stain components microscopically. That is after all the purpose of being a Histologist. I am going out on a limb here and I normally don't, but you are digging yourself in to a rather rude hole to insult so many professional Histologist. Just saying............. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 01/26/2010 07:38 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under > the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. 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Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 27 Jan 2010 10:25:16 -0600 From: "Cory Collins" Subject: [Histonet] Pathology Laboratory Manager Opening in Houston, Texas To: Message-ID: Content-Type: text/plain; charset="us-ascii" SightLine is expanding their service lines and is seeking a pathology laboratory manager who will be responsible for the pathology laboratory services from the design and construction of the new facility, equipment purchasing, hiring staff, policies and procedures, certification and licensure. The ideal candidate must have managed laboratory staff and have been through CLIA certification. They must also have the ability to step-in as a laboratory technician should the situation demanded it. The new facility will be located on South Main near the Reliant Stadium in Houston, Texas Reports to Vice President of Operations. Education: Bachelor Degree in Science with appropriate on the job training. Please indicate your interest by sending emails with resumes attached to hr@sightlinehealth.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 74, Issue 32 **************************************** From jkiernan <@t> uwo.ca Wed Jan 27 12:09:17 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Jan 27 12:09:21 2010 Subject: [Histonet] Coverslipping In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46DD9@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46DC7@nmdamailsvr.nmda.ad.nmsu.edu> <4D14F0FC9316DD41972D5F03C070908B02E46DD9@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: You're right of course, about the way you are taught being correct! My justification for coverslipping thicker objects by the slide-on-table method is that it's easier to control the amount of mounting medium, so that there's more of it over any holes and between the object and where the edge of the coverslip will be. If a slide with a thick object on it is lowered onto a coverslip with a slug of mountant on it, there will often be bubbles, and even more often bays of air forming as the solvent evaporates. (My thick objects have mostly been whole-mounts of layers dissected from rat or mouse intestine, containing the myenteric plexus sandwiched between the longitudinal and circular smooth muscle layers. These rectangular specimens can be up to 2X3cm, and they sometimes try to curl up into cylinders again, even after staining, dehydration and clearing.) John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Breeden, Sara" Date: Wednesday, January 27, 2010 7:58 Subject: RE: [Histonet] Coverslipping To: John Kiernan jkiernan@uwo.ca > It?s just that I was taught the ?place the coverslip ON the slide?, not ?place the slide ON the coverslip? and as one knows, the way you are taught is the way you think is correct. I?ve done it this way for so long that it would cause my fingers to have major emotional problems! It?s like the pronunciation of ?tomato? or ?potato?? I would think that the ?place the slide ON the coverslip? method would be more acceptable in, say, Australia (because the country is on the ?bottom? of the world? J > > What is your justification for the ?backward? way of coverslipping for sections less than 50 um? > > Sally Breeden, HT(ASCP) > NM Dept. of Agriculture > Veterinary Diagnostic Services > PO Box 4700 > Albuquerque, NM 87106 > 505-841-2576 > From ndevans <@t> stanford.edu Wed Jan 27 12:25:42 2010 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Wed Jan 27 12:25:47 2010 Subject: [HISTONET] embedding cell cultures In-Reply-To: <86091537.2476571264616180491.JavaMail.root@zm06.stanford.edu> Message-ID: <1776573639.2479441264616742541.JavaMail.root@zm06.stanford.edu> Dear all, I was hoping someone might be able to offer me some advice on embedding and sectioning cell cultures. In short we are growing cells which form 3D dome-like structures on tissue culture plastic. Does anyone have any experience or advice to offer on embedding the cultures in situ before sectioning? I have seen various methods in the literature, which often use Epon to embed the material followed by sawing away the plastic, but if anyone can offer some tips on other possible (easier) ways of doing it, or can refer me to some useful literature, I'd be very grateful. I would like to have simple 10um sections at 90 degrees to the substrate, which I can use for IHC. Best wishes Nick From mcauliff <@t> umdnj.edu Wed Jan 27 14:19:36 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Jan 27 14:17:29 2010 Subject: [HISTONET] embedding cell cultures In-Reply-To: <1776573639.2479441264616742541.JavaMail.root@zm06.stanford.edu> References: <1776573639.2479441264616742541.JavaMail.root@zm06.stanford.edu> Message-ID: <4B609FD8.7030303@umdnj.edu> Hi Nick: You can use the Epon substitutes such as EmBed 812. Fix, osmicate, dehydrate as usual, but omit the proplylene oxide as it will react with the plastic dish. Epon substitutes will mix with ethanol. I used 2:1 then 1:1 then 1:2 ratios of absolute ethanol to "Epon" with catalyst added with agitation. Then several changes of pure Epon and polymerize. Yes, you will have to saw away the plastic, if you try to section the Epon-plastic combo the two will separate. How much easier do you want it to be? Geoff Nicholas David Evans wrote: > Dear all, > > I was hoping someone might be able to offer me some advice on embedding and sectioning cell cultures. > > In short we are growing cells which form 3D dome-like structures on tissue culture plastic. Does anyone have any experience or advice to offer on embedding the cultures in situ before sectioning? I have seen various methods in the literature, which often use Epon to embed the material followed by sawing away the plastic, but if anyone can offer some tips on other possible (easier) ways of doing it, or can refer me to some useful literature, I'd be very grateful. > > I would like to have simple 10um sections at 90 degrees to the substrate, which I can use for IHC. > > Best wishes > Nick > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From victor <@t> pathology.washington.edu Wed Jan 27 14:27:04 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Jan 27 14:28:08 2010 Subject: [Histonet] Leica Bond 3 Message-ID: <4B60A198.9050709@pathology.washington.edu> We are currently demoing the Bond 3. Has anyone else tried this unit? Sounds like the closest competitor is Biocare and I would be interested in any comments. Considering our current volume and workflow, I don't see the Bond as being able to keep up. I don't work directly in the Immuno lab, but I have a Histology background. Thanks Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From MSHERWOOD <@t> PARTNERS.ORG Wed Jan 27 14:39:05 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jan 27 14:39:11 2010 Subject: [HISTONET] embedding cell cultures In-Reply-To: <4B609FD8.7030303@umdnj.edu> References: <1776573639.2479441264616742541.JavaMail.root@zm06.stanford.edu> <4B609FD8.7030303@umdnj.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F94@PHSXMB30.partners.org> Nick, I am assuming that your 3D cells only grow on plastic. They make plastic cover slips which, if your cells attach to them and grow, might make the embedding much easier. You would follow the same method as stated, but then you could invert the coverslips on a beem capsule and separate the coverslip from capsule with liquid nitrogen. However, I have never done it with plastic coverslips (only glass), so not sure if they would easily separate from capsule with liquid nitrogen. If anyone else has done so, please weigh in. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Wednesday, January 27, 2010 3:20 PM To: Nicholas David Evans Cc: histonet@lists.utsouthwestern.edu Subject: Re: [HISTONET] embedding cell cultures Hi Nick: You can use the Epon substitutes such as EmBed 812. Fix, osmicate, dehydrate as usual, but omit the proplylene oxide as it will react with the plastic dish. Epon substitutes will mix with ethanol. I used 2:1 then 1:1 then 1:2 ratios of absolute ethanol to "Epon" with catalyst added with agitation. Then several changes of pure Epon and polymerize. Yes, you will have to saw away the plastic, if you try to section the Epon-plastic combo the two will separate. How much easier do you want it to be? Geoff Nicholas David Evans wrote: > Dear all, > > I was hoping someone might be able to offer me some advice on embedding and sectioning cell cultures. > > In short we are growing cells which form 3D dome-like structures on tissue culture plastic. Does anyone have any experience or advice to offer on embedding the cultures in situ before sectioning? I have seen various methods in the literature, which often use Epon to embed the material followed by sawing away the plastic, but if anyone can offer some tips on other possible (easier) ways of doing it, or can refer me to some useful literature, I'd be very grateful. > > I would like to have simple 10um sections at 90 degrees to the substrate, which I can use for IHC. > > Best wishes > Nick > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From mbisher <@t> Princeton.EDU Wed Jan 27 14:44:37 2010 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Wed Jan 27 14:44:47 2010 Subject: [HISTONET] embedding cell cultures In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23F94@PHSXMB30.partners.org> Message-ID: I have done just what you are talking about using Aclar. It is a plastic embedding film (purchased from EMS). It works great for us. Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu On 1/27/10 3:39 PM, "Sherwood, Margaret" wrote: > Nick, > > I am assuming that your 3D cells only grow on plastic. They make plastic > cover > slips which, if your cells attach to them and grow, might make the embedding > much easier. You would follow the same method as stated, but then you could > invert the coverslips on a beem capsule and separate the coverslip from > capsule > with liquid nitrogen. However, I have never done it with plastic coverslips > (only glass), so not sure if they would easily separate from capsule with > liquid > nitrogen. If anyone else has done so, please weigh in. > > Peggy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff > McAuliffe > Sent: Wednesday, January 27, 2010 3:20 PM > To: Nicholas David Evans > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [HISTONET] embedding cell cultures > > Hi Nick: > > You can use the Epon substitutes such as EmBed 812. Fix, osmicate, > dehydrate as usual, but omit the proplylene oxide as it will react with > the plastic dish. Epon substitutes will mix with ethanol. I used 2:1 > then 1:1 then 1:2 ratios of absolute ethanol to "Epon" with catalyst > added with agitation. Then several changes of pure Epon and polymerize. > Yes, you will have to saw away the plastic, if you try to section the > Epon-plastic combo the two will separate. > How much easier do you want it to be? > > Geoff > > Nicholas David Evans wrote: >> Dear all, >> >> I was hoping someone might be able to offer me some advice on embedding and > sectioning cell cultures. >> >> In short we are growing cells which form 3D dome-like structures on tissue > culture plastic. Does anyone have any experience or advice to offer on > embedding > the cultures in situ before sectioning? I have seen various methods in the > literature, which often use Epon to embed the material followed by sawing away > the plastic, but if anyone can offer some tips on other possible (easier) ways > of doing it, or can refer me to some useful literature, I'd be very grateful. >> >> I would like to have simple 10um sections at 90 degrees to the substrate, > which I can use for IHC. >> >> Best wishes >> Nick >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > From rjbuesa <@t> yahoo.com Wed Jan 27 14:48:37 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 27 14:48:42 2010 Subject: [Histonet] 20% NaOH Nail pre-Treatment... Questions In-Reply-To: Message-ID: <853736.98667.qm@web65715.mail.ac4.yahoo.com> 20% NaOH is too strong. Under separate cover I am sending another procedure. Ren? J. --- On Tue, 1/26/10, Due, Brice wrote: From: Due, Brice Subject: [Histonet] 20% NaOH Nail pre-Treatment... Questions To: "Joe "The Toe" Nocito" Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, January 26, 2010, 6:05 PM Hello Joe, first thanks so much for sharing your expertise here on Histonet. I work in the main histo lab at Mass General Hosp. in Boston and I have interested the powers that be in your NaOH protocol. I initially used your NaOH idea to attach an "impossible" nail to slides by floating 4um section on 20% NaOH for 10-15min. (The transfers between regular water bath and NaOH bath (and back to rinse) were surprisingly easy because the concentrated NaOH prevents adhesion to even the stickiest adhesive slides.) Anyhow, I have some questions for you about your lab's implementation. I found your most complete post at http://lists.utsouthwestern.edu/pipermail/histonet/2008-October/040078.html But I am concerned about the safety of 20% NaOH in routine use. Would you mind posting or emailing me any SOPs you have that address the safety issues? Specifically both the histology lab and the "grossing" labs here use RDO decal solutions at every bench. So there is/will be concern about the proper handling of 20% NaOH around RDO... by overworked PAs and residents, etc. etc. Feel free to tell me your safety almost-horror stories so we can (try to) prevent them from occuring here. I've seen that some people use 10% NaOH instead. Have you experimented with this? Any reasons you stick with 20% beyond the usual "it ain't broke, so..."? Have you (or anyone on Histonet) ever tried to find the minimum effective concentration? Increasing the pre-soak time isn't an issue here since I've been assured that nail turnaround times are non-critical. Along these lines, do you or anyone have any histochemical references for the reactions that are taking place? With this info it might be possible to predict the minimum effective pH. It would also be nice to have references for the SOP. (I know about the coverslip "crushes" some derm clinicians do with fresh scrapings. Alternately, do you know of any histochemical references for this?) Thank you so much for sharing your time and info! And if I happen to answer any of my own questions I will be sure to post what I learn. Sincerely, -brice Massachusetts General Hospital Surgical Pathology, Histology The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 27 14:57:17 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 27 14:57:22 2010 Subject: [Histonet] Dako vs Ventana In-Reply-To: <768327.23434.qm@web65709.mail.ac4.yahoo.com> Message-ID: <382848.10373.qm@web65716.mail.ac4.yahoo.com> Dako Ren? J. --- On Wed, 1/27/10, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] Dako vs Ventana To: "histonet" Date: Wednesday, January 27, 2010, 10:06 AM Hello all, I am trying to get a feel for some of the special stainers out there.? I came to the conclusion that it will be either Dako or Ventana.? Opinions?? Any other machines on the market that people love?? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Jan 27 14:59:22 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jan 27 14:59:32 2010 Subject: [Histonet] Leica Bond 3 In-Reply-To: <4B60A198.9050709@pathology.washington.edu> References: <4B60A198.9050709@pathology.washington.edu> Message-ID: <4B6062D9.7400.0077.1@harthosp.org> We have a Bond III and we love it. I don't usually use the word "love" for an instrument, but, in this case, it applies. We do multiple runs on it every day. We were a pilot site for Leica-Microsystems and at the end of the test period I told them that I didn't want this instrument to leave. It is now part of our operation (we also have 5 Bond Max instruments). Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Victor Tobias 1/27/2010 3:27 PM >>> We are currently demoing the Bond 3. Has anyone else tried this unit? Sounds like the closest competitor is Biocare and I would be interested in any comments. Considering our current volume and workflow, I don't see the Bond as being able to keep up. I don't work directly in the Immuno lab, but I have a Histology background. Thanks Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisdx.com Wed Jan 27 14:59:36 2010 From: mhale <@t> carisdx.com (Hale, Meredith) Date: Wed Jan 27 14:59:39 2010 Subject: [Histonet] Dako vs Ventana Message-ID: <6F33D8418806044682A391273399860F019246D6@s-irv-ex301.PathologyPartners.intranet> Ventana works great !!! ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet ; kristen arvidson Sent: Wed Jan 27 14:57:17 2010 Subject: Re: [Histonet] Dako vs Ventana Dako Ren? J. --- On Wed, 1/27/10, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] Dako vs Ventana To: "histonet" Date: Wednesday, January 27, 2010, 10:06 AM Hello all, I am trying to get a feel for some of the special stainers out there.? I came to the conclusion that it will be either Dako or Ventana.? Opinions?? Any other machines on the market that people love?? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 27 15:02:50 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 27 15:02:57 2010 Subject: [Histonet] Re: Diff-Quik In-Reply-To: Message-ID: <941502.74110.qm@web65704.mail.ac4.yahoo.com> If not sure about the worthiness, in-subscribe (you can do it yourself). Ren? J. --- On Wed, 1/27/10, Kim.Donadio@bhcpns.org wrote: From: Kim.Donadio@bhcpns.org Subject: RE: [Histonet] Re: Diff-Quik To: "Mike Pence" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu, "Robert Richmond" Date: Wednesday, January 27, 2010, 11:17 AM Thanks for your profound attitude. A "sort of apology" is like a boat that "sort of floats" . I am surprised at the unprofessional comments that get sent to my desk from here every day. Not sure if it is really worth it. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Mike Pence" 01/27/2010 10:09 AM To , "Robert Richmond" cc , Subject RE: [Histonet] Re: Diff-Quik Give it a rest!? Dr. Richmond 'sort of apologized' for his comments. He does explain his views and why he stated them. That should be the end of it! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, January 27, 2010 9:53 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Diff-Quik I'm going to have to agree with Cheryl on the comment. This may be your experience but I can tell you my techs always look at their stains before they send it on to the Pathologist. It is a requirement that they understand what they are looking at in order to know if it worked. Each of them are also trained to know all tissues microscopically and all stain components microscopically. That is after all the purpose of being a Histologist. I am going out on a limb here and I normally don't, but you are digging yourself in to a rather rude hole to insult so many professional Histologist. Just saying............. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 01/26/2010 07:38 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under > the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers.? I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. 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Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Jan 27 15:10:25 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jan 27 15:10:31 2010 Subject: [Histonet] Leica Bond 3 In-Reply-To: <4B6062D9.7400.0077.1@harthosp.org> Message-ID: How long have you had the Bond III? During this time, how many service calls have you had for it? I put out a post a few days ago about service packages for the Bond and the Peloris. No one has responded about the Bond yet. Does this mean that they have been problem free? I did have a few responses from Peloris users who had many service calls. Are all of them prone to problems? I'd like to hear from users who have the Peloris and have not had problems with it. Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Wednesday, January 27, 2010 3:59 PM To: Histonet; Victor Tobias Subject: Re: [Histonet] Leica Bond 3 We have a Bond III and we love it. I don't usually use the word "love" for an instrument, but, in this case, it applies. We do multiple runs on it every day. We were a pilot site for Leica-Microsystems and at the end of the test period I told them that I didn't want this instrument to leave. It is now part of our operation (we also have 5 Bond Max instruments). Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Victor Tobias 1/27/2010 3:27 PM >>> We are currently demoing the Bond 3. Has anyone else tried this unit? Sounds like the closest competitor is Biocare and I would be interested in any comments. Considering our current volume and workflow, I don't see the Bond as being able to keep up. I don't work directly in the Immuno lab, but I have a Histology background. Thanks Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. 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From Barbara.Crill <@t> LPNT.net Wed Jan 27 15:20:24 2010 From: Barbara.Crill <@t> LPNT.net (Crill Antoinette) Date: Wed Jan 27 15:20:28 2010 Subject: [Histonet] HISTO SAFETY GLOVES Message-ID: <7DA79EBDBD92BF408EF392413737878D390B85FA09@NADCWPMSGCMS01.hca.corpad.net> Is anyone using Tplus gloves as part of their procedure for changing the cryostat or microtome blades? ANTOINETTE CRILL, MBA,CT(ASCP) From eberledelphine <@t> hotmail.com Wed Jan 27 15:27:30 2010 From: eberledelphine <@t> hotmail.com (delphine eberle) Date: Wed Jan 27 15:27:34 2010 Subject: [Histonet] Stains for Macrophages for Laser Capture Microdissection purpose In-Reply-To: References: <4E17A1A86498044BA9E35001C8B7C6038391973F@ushpwbmsmmp008.one.ads.bms.com>, Message-ID: Hi, I have another question following Macrophage staining. I am setting up a Laser Capture Microdissection protocol to dissect macrophages from atherosclerosis lesions (non fixed frozen sections) and extract RNA for gene expression purpose. I am looking at a quick staining (less than 30min otherwise RNA is degradated) for macrophages in that context? Any suggestions? Thanks a lot, Delphine Delphine Eberl? PhD UCSF Department of Vascular Surgery eberledelphine@hotmail.com > From: jkiernan@uwo.ca > To: sharon.willman@bms.com > Date: Wed, 27 Jan 2010 00:22:53 -0500 > Subject: Re: [Histonet] Histology Special Stains for Macrophages > CC: histonet@lists.utsouthwestern.edu > > None of the methods mentioned in the enquiry are stains for macrophages. Research workers who never took Histology 101 often stain cells of the monocyte/macrophage lineage immunohistochemically (IHC), using very expensive primary antibodies and fairly expensive kits to amplify and detect the binding sites. IHC is necessary if you must find every macrophage, including a tissue's recent monocyte immigrants that haven't yet done any work. Macrophages that have been busily eating blood are full of brown granules that don't need any staining. > > Ordinary people recognize macrophages by their appearance in sections stained with a well done H&E or with one of the many Romanowsky-Giemsa blood stains. With the latter it's important to get the pH right - much lower for sections of formaldehyde-fixed objects (pH4 to 5) than for methanol-fixed films or smears (traditionally 6.8). It's all very well explained in RD Lillie & GM Fullmer's "Histopathologic Technic..." (4th ed 1976, pp.193-197; the 3rd edition, 1965, is OK too) and also in more recent textbooks including those by Bancroft & Gamble, Geoffrey Brown, Freida Carson and, of course > Yours truly. > > John Kiernan > Anatomy, UWO > London, Canada > http://publish.uwo.ca/~jkiernan/bookfind.htm > = = = > ----- Original Message ----- > From: "Willman, Sharon" > Date: Tuesday, January 26, 2010 12:12 > Subject: [Histonet] Histology Special Stains for Macrophages > To: "histonet@lists.utsouthwestern.edu" > > > Hi, > > We are needing to do a special stain for macrophages. What > > is the most common stain for that? Does anyone do a Sudan > > Black, Alcian Blue or Van Gieson for macrophages? Any > > information would be appreciated. > > Thanks in advance. > > Sharon > > > > > > ________________________________ > > This message (including any attachments) may contain > > confidential, proprietary, privileged and/or private > > information. The information is intended to be for the use of > > the individual or entity designated above. If you are not the > > intended recipient of this message, please notify the sender > > immediately, and delete the message and any attachments. Any > > disclosure, reproduction, distribution or other use of this > > message or any attachments by an individual or entity other than > > the intended recipient is prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ T?l?chargez Internet Explorer 8 et surfez sans laisser de trace ! http://clk.atdmt.com/FRM/go/182932252/direct/01/ From rjbuesa <@t> yahoo.com Wed Jan 27 15:51:30 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 27 15:51:35 2010 Subject: [Histonet] Stains for Macrophages for Laser Capture Microdissection purpose In-Reply-To: Message-ID: <635443.45626.qm@web65716.mail.ac4.yahoo.com> Toluidine blue. Ren? J. --- On Wed, 1/27/10, delphine eberle wrote: From: delphine eberle Subject: [Histonet] Stains for Macrophages for Laser Capture Microdissection purpose To: jkiernan@uwo.ca, sharon.willman@bms.com Cc: histonet@lists.utsouthwestern.edu Date: Wednesday, January 27, 2010, 4:27 PM Hi, I have another question following Macrophage staining. I am setting up a Laser Capture Microdissection protocol to dissect macrophages from atherosclerosis lesions (non fixed frozen sections) and extract RNA for gene expression purpose. I am looking at a quick staining (less than 30min otherwise RNA is degradated) for macrophages in that context? Any suggestions? Thanks a lot, Delphine Delphine Eberl? PhD UCSF Department of Vascular Surgery eberledelphine@hotmail.com > From: jkiernan@uwo.ca > To: sharon.willman@bms.com > Date: Wed, 27 Jan 2010 00:22:53 -0500 > Subject: Re: [Histonet] Histology Special Stains for Macrophages > CC: histonet@lists.utsouthwestern.edu > > None of the methods mentioned in the enquiry are stains for macrophages. Research workers who never took Histology 101 often stain cells of the monocyte/macrophage lineage immunohistochemically (IHC), using very expensive primary antibodies and fairly expensive kits to amplify and detect the binding sites. IHC is necessary if you must find every macrophage, including a tissue's recent monocyte immigrants that haven't yet done any work. Macrophages that have been busily eating blood are full of brown granules that don't need any staining. > > Ordinary people recognize macrophages by their appearance in sections stained with a well done H&E or with one of the many Romanowsky-Giemsa blood stains. With the latter it's important to get the pH right - much lower for sections of formaldehyde-fixed objects (pH4 to 5) than for methanol-fixed films or smears (traditionally 6.8). It's all very well explained in RD Lillie & GM Fullmer's "Histopathologic Technic..." (4th ed 1976, pp.193-197; the 3rd edition, 1965, is OK too) and also in more recent textbooks including those by Bancroft & Gamble, Geoffrey Brown, Freida Carson and, of course > Yours truly. > > John Kiernan > Anatomy, UWO > London, Canada > http://publish.uwo.ca/~jkiernan/bookfind.htm > = = = > ----- Original Message ----- > From: "Willman, Sharon" > Date: Tuesday, January 26, 2010 12:12 > Subject: [Histonet] Histology Special Stains for Macrophages > To: "histonet@lists.utsouthwestern.edu" > > > Hi, > > We are needing to do a special stain for macrophages. What > > is the most common stain for that? Does anyone do a Sudan > > Black, Alcian Blue or Van Gieson for macrophages? Any > > information would be appreciated. > > Thanks in advance. > > Sharon > > > > > > ________________________________ > > This message (including any attachments) may contain > > confidential, proprietary, privileged and/or private > > information. The information is intended to be for the use of > > the individual or entity designated above. If you are not the > > intended recipient of this message, please notify the sender > > immediately, and delete the message and any attachments. Any > > disclosure, reproduction, distribution or other use of this > > message or any attachments by an individual or entity other than > > the intended recipient is prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ???????? ?????? ??? ? _________________________________________________________________ T?l?chargez Internet Explorer 8 et surfez sans laisser de trace ! http://clk.atdmt.com/FRM/go/182932252/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Wed Jan 27 15:57:16 2010 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Jan 27 15:57:51 2010 Subject: [Histonet] Re: Diff-Quik In-Reply-To: References: Message-ID: <26938660D7E848FAA055BCB0A76DF8BB@BryanPC> I have worked in several laboratories, both in Canada and England. I trained there and was taught by technologists, not pathologists, to check the staining of all my slides, which I have always done. I finds that even when asked, most pathologists do not return poorly stained slides, yet how can we know of deficiencies if they do not? I worked in several histology labs over my career and slides were checked in all but two. I was able to introduce it in one of those, but not in the other due to technologist resistance (we've always done it that way). It was the technologists who resisted it. I don't see why Dr. Richmond needs to apologise. He merely drew attention to his work experiences in numerous histology labs and decried what is, in his experience, a common practice. In other words, he spoke about real, actual events. Why does being truthful require an apology? Are our egos so fragile that we can't take valid criticism as a profession nor as individuals? He certainly did not accuse those who got upset of failing to check slides, so why take umbrage? Noting and decrying poor histological practices should be a concern for everyone. Surely we are concerned about service to patients not about our egos. Bryan Llewellyn From BDUE <@t> PARTNERS.ORG Wed Jan 27 16:14:58 2010 From: BDUE <@t> PARTNERS.ORG (Due, Brice) Date: Wed Jan 27 16:15:02 2010 Subject: [Histonet] 20% NaOH Nail pre-Treatment... Questions In-Reply-To: <853736.98667.qm@web65715.mail.ac4.yahoo.com> References: <853736.98667.qm@web65715.mail.ac4.yahoo.com> Message-ID: Hello Rene, thank you very much for the paper you sent me! I will read it tomorrow. > 20% NaOH is too strong. Actually, floating 4um nail sections on 20% NaOH does work. Very well. We just validated it today and the attending/staff derm path people are very happy with the results. We will continue to "float" difficult nails until we implement and validate a pre-soak protocol in the grossing lab. The key to making foatation work well is to re-float the sections on a regular water bath for several minutes to rinse out the NaOH. Residual NaOH will prevent adhesion to even the stickiest slides. My guess is the keratin network is crosslinked in many ways, and the NaOH treatment is degrading only some of them. What I have observed with 4um sections is they flatten out and "spread" while floating on NaOH -- very similar to how nail clippings swell up when soaked in 20% NaOH. Of course this can go too far and leave you with mush, but 4um sections can certainly withstand 20% NaOH (warm ~40C, not RT) for at least 15min. The derm pathologist said the morphology looked good. There's always more than one way... :) Thanks again for the paper! -brice ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, January 27, 2010 3:49 PM To: Joe The Toe Nocito; Due, Brice Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 20% NaOH Nail pre-Treatment... Questions 20% NaOH is too strong. Under separate cover I am sending another procedure. Ren? J. --- On Tue, 1/26/10, Due, Brice wrote: From: Due, Brice Subject: [Histonet] 20% NaOH Nail pre-Treatment... Questions To: "Joe "The Toe" Nocito" Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, January 26, 2010, 6:05 PM Hello Joe, first thanks so much for sharing your expertise here on Histonet. I work in the main histo lab at Mass General Hosp. in Boston and I have interested the powers that be in your NaOH protocol. I initially used your NaOH idea to attach an "impossible" nail to slides by floating 4um section on 20% NaOH for 10-15min. (The transfers between regular water bath and NaOH bath (and back to rinse) were surprisingly easy because the concentrated NaOH prevents adhesion to even the stickiest adhesive slides.) Anyhow, I have some questions for you about your lab's implementation. I found your most complete post at http://lists.utsouthwestern.edu/pipermail/histonet/2008-October/040078.html But I am concerned about the safety of 20% NaOH in routine use. Would you mind posting or emailing me any SOPs you have that address the safety issues? Specifically both the histology lab and the "grossing" labs here use RDO decal solutions at every bench. So there is/will be concern about the proper handling of 20% NaOH around RDO... by overworked PAs and residents, etc. etc. Feel free to tell me your safety almost-horror stories so we can (try to) prevent them from occuring here. I've seen that some people use 10% NaOH instead. Have you experimented with this? Any reasons you stick with 20% beyond the usual "it ain't broke, so..."? Have you (or anyone on Histonet) ever tried to find the minimum effective concentration? Increasing the pre-soak time isn't an issue here since I've been assured that nail turnaround times are non-critical. Along these lines, do you or anyone have any histochemical references for the reactions that are taking place? With this info it might be possible to predict the minimum effective pH. It would also be nice to have references for the SOP. (I know about the coverslip "crushes" some derm clinicians do with fresh scrapings. Alternately, do you know of any histochemical references for this?) Thank you so much for sharing your time and info! And if I happen to answer any of my own questions I will be sure to post what I learn. Sincerely, -brice Massachusetts General Hospital Surgical Pathology, Histology The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NLinke <@t> mednet.ucla.edu Wed Jan 27 16:25:12 2010 From: NLinke <@t> mednet.ucla.edu (Linke, Noelle) Date: Wed Jan 27 16:25:18 2010 Subject: [Histonet] Ventana Ultra users Message-ID: <0C96F0BFE078D74C91A1C541D24A6AE49CB9308A@EMGMB1.ad.medctr.ucla.edu> Hi all, Could those of you who use Ultras in your labs, please contact me offline? I am taking an unofficial survey..... Thank you, Noelle No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services UCLA Department of Pathology and Laboratory Medicine Phone: 310-825-7397 Fax: 310-983-3289 nlinke@mednet.ucla.edu Pager: 97471 ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From CIngles <@t> uwhealth.org Wed Jan 27 17:01:14 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Jan 27 17:03:18 2010 Subject: [Histonet] tissue retention...again Message-ID: Hey there: I would like to take a quick poll of all techs doing Mohs sections out there. I would like to know what you do with your tissue after being frozen sectioned, how long you keep it, how you store it, etc. Thanks Claire From pathmaster <@t> yahoo.com Wed Jan 27 18:38:08 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Wed Jan 27 18:38:11 2010 Subject: [Histonet] Diff Quik Message-ID: <185716.25257.qm@web111114.mail.gq1.yahoo.com> Dr Richmond, ? Wow- what a tangent. When I asked if anyone has compared the bothersome Romanowski stains and any of the?ready to use, practically?idiot proof Diff-Quik preparations, I didn't expect to ? Not to pile on or anything, I feel for you. I am sorry your are forced to toil away in numerous uncooperative?backwater minimalist labs, but if you have trouble in that arena (BM's), ?you should try the Diff Quik for bone marrows, it's so easy even a pathologist can do it. LOL ? Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant and provacateur who looks at every stain everyday and shows his techs, who hunger for knowlege, new things every day ? From jkiernan <@t> uwo.ca Wed Jan 27 21:41:15 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Jan 27 21:41:20 2010 Subject: [Histonet] Re: Stains for Macrophages for Laser Capture Microdissection purpose Message-ID: How about phase contrast optics and identifying the cells by their shapes? Non-specific addition of colour to a section of unfixed tissue probably will not show the cells any more clearly. I have taken the liberty of including a colleague, Dr Paul Walton, in this reply. He does laser capture microdissection and may have something to suggest. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: delphine eberle Date: Wednesday, January 27, 2010 16:27 Subject: Stains for Macrophages for Laser Capture Microdissection purpose To: jkiernan@uwo.ca, sharon.willman@bms.com Cc: histonet@lists.utsouthwestern.edu > Hi, > > I have another question following Macrophage staining. > I am setting up a Laser Capture Microdissection protocol to dissect macrophages from atherosclerosis lesions (non fixed frozen sections) and extract RNA for gene expression purpose. > I am looking at a quick staining (less than 30min otherwise RNA is degradated) for macrophages in that context? Any suggestions? > > Thanks a lot, > Delphine > > Delphine Eberl? PhD > UCSF Department of Vascular Surgery > eberledelphine@hotmail.com > > > From: jkiernan@uwo.ca > > To: sharon.willman@bms.com > > Date: Wed, 27 Jan 2010 00:22:53 -0500 > > Subject: Re: [Histonet] Histology Special Stains for Macrophages > > CC: histonet@lists.utsouthwestern.edu > > > > None of the methods mentioned in the enquiry are stains for macrophages. Research workers who never took Histology 101 often stain cells of the monocyte/macrophage lineage immunohistochemically (IHC), using very expensive primary antibodies and fairly expensive kits to amplify and detect the binding sites. IHC is necessary if you must find every macrophage, including a tissue's recent monocyte immigrants that haven't yet done any work. Macrophages that have been busily eating blood are full of brown granules that don't need any staining. > > > > Ordinary people recognize macrophages by their appearance in sections stained with a well done H&E or with one of the many Romanowsky-Giemsa blood stains. With the latter it's important to get the pH right - much lower for sections of formaldehyde-fixed objects (pH4 to 5) than for methanol-fixed films or smears (traditionally 6.8). It's all very well explained in RD Lillie & GM Fullmer's "Histopathologic Technic..." (4th ed 1976, pp.193-197; the 3rd edition, 1965, is OK too) and also in more recent textbooks including those by Bancroft & Gamble, Geoffrey Brown, Freida Carson and, of course > > Yours truly. > > > > John Kiernan > > Anatomy, UWO > > London, Canada > > http://publish.uwo.ca/~jkiernan/bookfind.htm > > = = = > > ----- Original Message ----- > > From: "Willman, Sharon" > > Date: Tuesday, January 26, 2010 12:12 > > Subject: [Histonet] Histology Special Stains for Macrophages > > To: "histonet@lists.utsouthwestern.edu" > > > > > Hi, > > > We are needing to do a special stain for macrophages. What > > > is the most common stain for that? Does anyone do a Sudan > > > Black, Alcian Blue or Van Gieson for macrophages? Any > > > information would be appreciated. > > > Thanks in advance. > > > Sharon > > > > > > > > > ________________________________ > > > This message (including any attachments) may contain > > > confidential, proprietary, privileged and/or private > > > information. The information is intended to be for the use of > > > the individual or entity designated above. If you are not the > > > intended recipient of this message, please notify the sender > > > immediately, and delete the message and any attachments. Any > > > disclosure, reproduction, distribution or other use of this > > > message or any attachments by an individual or entity other than > > > the intended recipient is prohibited. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Vous cherchez l'int?grale des clips de Michael Jackson ? Bing ! Trouvez ! From rsrichmond <@t> gmail.com Thu Jan 28 07:41:44 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Jan 28 07:41:48 2010 Subject: [Histonet] Microtomist's Vade-Mecum on the Web Message-ID: Arthur Bolles Lee's The Microtomist's Vade-Mecum, 7th edition (1913) is available online on Google Books, along with some other editions: http://books.google.com/books?id=4m5MAAAAMAAJ&printsec=frontcover&dq=arthur+bolles+lee&ei=BpJhS96xKJTIzASV9vnNBQ&cd=1#v=onepage&q=&f=false This marvelous old histology recipe book was a great rarity before the Web, though it's actually now available from Amazon. It took me several years to find a copy in 1976 - found one in a consignment of old books discarded from the library of a long-defunct college. A note about the title: Vade-Mecum (pronounced something like voddy-make-um) - literally "come with me" (think of "Quo Vadis) is an old word for a practical handbook of something. Bob Richmond Samurai Pathologist Knoxville TN From KPercival <@t> wyeth.com Thu Jan 28 07:57:08 2010 From: KPercival <@t> wyeth.com (Percival Karen) Date: Thu Jan 28 07:57:24 2010 Subject: [Histonet] Re: Stains for Macrophages for Laser CaptureMicrodissection purpose In-Reply-To: References: Message-ID: <4B61516402000011001F80E9@gv01a67m.gv.us.pri.wyeth.com> Delphine, I think John is right about identifying the cells by shape. I do LCM, and I perform a rapid hematoxylin stain just for orientation purposes. The total time from fixation to air drying is 7 minutes. Any more time than that, and the RNA is too degraded to get good results from downstream applications. You could substitute toluidine blue for the hematoxylin in this case. Are you really looking for a (no greater than) 30 minute (aqueous) stain? Seems way too long for good quality and quantity RNA. I would be interested in hearing what you usually do for staining for LCM purposes and what your RNA results have been. Karen Karen Percival, BS, HT Research Scientist II Pfizer Research DSRD 1 Burtt Road G3025 Andover, MA 01810 888-577-1500 x 4058 kpercival @wyeth.com >>> John Kiernan 1/27/2010 10:41 PM >>> How about phase contrast optics and identifying the cells by their shapes? Non-specific addition of colour to a section of unfixed tissue probably will not show the cells any more clearly. I have taken the liberty of including a colleague, Dr Paul Walton, in this reply. He does laser capture microdissection and may have something to suggest. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: delphine eberle Date: Wednesday, January 27, 2010 16:27 Subject: Stains for Macrophages for Laser Capture Microdissection purpose To: jkiernan@uwo.ca, sharon.willman@bms.com Cc: histonet@lists.utsouthwestern.edu > Hi, > > I have another question following Macrophage staining. > I am setting up a Laser Capture Microdissection protocol to dissect macrophages from atherosclerosis lesions (non fixed frozen sections) and extract RNA for gene expression purpose. > I am looking at a quick staining (less than 30min otherwise RNA is degradated) for macrophages in that context? Any suggestions? > > Thanks a lot, > Delphine > > Delphine Eberl? PhD > UCSF Department of Vascular Surgery > eberledelphine@hotmail.com > > > From: jkiernan@uwo.ca > > To: sharon.willman@bms.com > > Date: Wed, 27 Jan 2010 00:22:53 -0500 > > Subject: Re: [Histonet] Histology Special Stains for Macrophages > > CC: histonet@lists.utsouthwestern.edu > > > > None of the methods mentioned in the enquiry are stains for macrophages. Research workers who never took Histology 101 often stain cells of the monocyte/macrophage lineage immunohistochemically (IHC), using very expensive primary antibodies and fairly expensive kits to amplify and detect the binding sites. IHC is necessary if you must find every macrophage, including a tissue's recent monocyte immigrants that haven't yet done any work. Macrophages that have been busily eating blood are full of brown granules that don't need any staining. > > > > Ordinary people recognize macrophages by their appearance in sections stained with a well done H&E or with one of the many Romanowsky-Giemsa blood stains. With the latter it's important to get the pH right - much lower for sections of formaldehyde-fixed objects (pH4 to 5) than for methanol-fixed films or smears (traditionally 6.8). It's all very well explained in RD Lillie & GM Fullmer's "Histopathologic Technic..." (4th ed 1976, pp.193-197; the 3rd edition, 1965, is OK too) and also in more recent textbooks including those by Bancroft & Gamble, Geoffrey Brown, Freida Carson and, of course > > Yours truly. > > > > John Kiernan > > Anatomy, UWO > > London, Canada > > http://publish.uwo.ca/~jkiernan/bookfind.htm > > = = = > > ----- Original Message ----- > > From: "Willman, Sharon" > > Date: Tuesday, January 26, 2010 12:12 > > Subject: [Histonet] Histology Special Stains for Macrophages > > To: "histonet@lists.utsouthwestern.edu" > > > > > Hi, > > > We are needing to do a special stain for macrophages. What > > > is the most common stain for that? Does anyone do a Sudan > > > Black, Alcian Blue or Van Gieson for macrophages? Any > > > information would be appreciated. > > > Thanks in advance. > > > Sharon > > > > > > > > > ________________________________ > > > This message (including any attachments) may contain > > > confidential, proprietary, privileged and/or private > > > information. The information is intended to be for the use of > > > the individual or entity designated above. If you are not the > > > intended recipient of this message, please notify the sender > > > immediately, and delete the message and any attachments. Any > > > disclosure, reproduction, distribution or other use of this > > > message or any attachments by an individual or entity other than > > > the intended recipient is prohibited. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Vous cherchez l'int?grale des clips de Michael Jackson ? Bing ! Trouvez ! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Thu Jan 28 08:38:31 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Jan 28 08:38:37 2010 Subject: [Histonet] Re: [IHCRG] Hamamatsu Nanozoomer In-Reply-To: References: <102966.22174.qm@web50306.mail.re2.yahoo.com> Message-ID: <976386.90905.qm@web50304.mail.re2.yahoo.com> I would like to thank everyone that responded to my questions about these scanners.??These answers have been very valuable to my boss and I and will be taken into account when we decide on which system to purchase! ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: James Watson To: Kim Merriam Cc: "Joel.Duckworth@OLYMPUS.COM" Sent: Thu, January 28, 2010 9:15:52 AM Subject: RE: [IHCRG] Hamamatsu Nanozoomer Kim, ? I use the nanozoomer a lot,? we have? scanned about 45,000 images in 3 years. I bought it because it does both fluorescence and transmitted light very well.? The aperio scanner is good and the software can view both types of scans.? The Nanzoomer software has improved greatly in the past 3 years thanks to Olympus and the service has been excellent.? I will send you some images and more information when I get to work.? I have CC?ed my sales rep,? he will make sure someone gets in touch with you today.?? The cost is about the same as a single instrument from Aperio (unless aperio changed their pricing). ? Jamie ? From:Kim Merriam [mailto:kmerriam2003@yahoo.com] Sent: Thursday, January 28, 2010 4:44 AM To: James Watson Subject: Re: [IHCRG] Hamamatsu Nanozoomer ? Thanks Jamie.??When I was at Novartis/NIBRI, I used the Aperio?quite a bit (I am at Amgen now, but I remember meeting you a while back at one of the NSH meetings).? Anyway,?the other Amgen sites use the Aperio, but we are intrigued?by the possibility of buying one machine that can do light and IF imaging, also the Z-stack feature is a nice ad-on (although I am not sure how much we would really use it).? Based on the information I have gathered, I would be able to pull the images taken from the Nanozoomer and put them on our Aperio server for cross-site conferences. ? We were actually ready to purchase the Aperio next week, but then my boss found out about the Nanozoomer, which seems to be a better instrument, but I have no idea how much it costs, I cant get anyone from Hamamatsu or Olympus to call me back. ? Kim ? ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ? ? ________________________________ From:James Watson To: "kmerriam2003@yahoo.com" Sent: Thu, January 28, 2010 1:08:16 AM Subject: FW: [IHCRG] Hamamatsu Nanozoomer Kim, Contact me,? I have a Nanozoomer and also know a lot about the Aperio since their headquarters is near here and I have been to their office many times and they have been here. Jamie James Watson HT? ASCP Facilities Manager of Histology GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, January 27, 2010 9:09 AM To: kmerriam2003@yahoo.com; Histonet; ihcrg@googlegroups.com Subject: [Histonet] RE: [IHCRG] Hamamatsu Nanozoomer Kim We have an Aperio Scanner.? I'm not quite sure what you are asking but this is what I think from your e-mail.? Aperio scans create .svs files these files are considered a modified tiff file.? When aperio scans the file is placed into the spectrum database - from there you can create case or project files, etc.? These images can be viewed via imagescope.? There is no issue with placing other images that have not been generated on the scanscope into the spectrum database, you can upload any image into the spectrum database we have done this in the past.? These images can also be viewed with imagescope we have only uploaded single magnification tiff files into the aperio spectrum database.? The Hamamatsu Nanozoomer I believe also comes with a database and some viewing sofware.? Are you asking if you can view the HN images in the spectrum database?? To be honest I'm not sure if you can view the images completed with image scope I would ask your sales rep.? I'm sure you are aware that both of these scanners essentially create virtual slides.? Not just an image of a slide at one magnification.? The viewing software allows you to view the images at different magnifications. I'm not that familar with Visiomorph its the Olympus product and doesn't Olympus sell the HN scanner?? I would also think that that the database that comes with the HN scanner should be able to accept other images and should have some conferencing capabilities, I know that the aperio does.? I'm not sure if I answered your questions, but I would ask the sales reps from both aperio and Olympus about compatability.? With repects to analysis I think that the Visiomorph software will be able to analyze a image generated from the scanscope.? If you are working with an HN scanner and the visiomorph software I'm assuming that you will be able to perform batch analysis on selected images from the olympus database.? You have to find out if you can run batch analysis out of either of the databases with the Visiomorph software - if you have lots of slides to analyze then setting them up one at a time is too labor intensive.? You can call me if you want to, I'm at a conference but will be back in the lab tomorrow? (303) 682-3949 Liz ________________________________ From: ihcrg@googlegroups.com on behalf of Kim Merriam Sent: Wed 1/27/2010 8:16 AM To: Histonet; ihcrg@googlegroups.com Subject: [IHCRG] Hamamatsu Nanozoomer Hi Everyone, I was wondering if any of you folks had experience with the Hamamatsu Nanozoomer slide scanner.? We recently learned about it and are considering getting one instead of an Aperio slide scanner.? We plan to use Visiomorph image analysis software, so all we really need is the scanner and not all of the fancy software that these companies sell to go with their instruments. The other labs at my company have Aperio systems and the images from the Nanozoomer would need to compatible or made to be compatabile for this to be a viable option for us (for cross-site slide conferencing purposes).? We are interested in it because this instrument can be adapted for fluorescence (with Aperio, you need to purchase 2 separate instruments; one for light microscope and one for fluorescence). Any insights would be greatly appreciated. Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Thu Jan 28 08:41:45 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Jan 28 08:41:51 2010 Subject: [Histonet] Re: Diff-Quik Message-ID: Dear Colleagues, At the risk of beating a dead horse, I would like to thank Bryan Llewellyn for so eloquently and succinctly echoing my own thoughts on the matter that Dr. Richmond commented on which has drawn criticism. For those who may have missed it, I'll include it below my own meager response. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO Date: Wed, 27 Jan 2010 13:57:16 -0800 From: "Bryan Llewellyn" Subject: Re: [Histonet] Re: Diff-Quik To: "Histonet" Message-ID: <26938660D7E848FAA055BCB0A76DF8BB@BryanPC> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I have worked in several laboratories, both in Canada and England. I trained there and was taught by technologists, not pathologists, to check the staining of all my slides, which I have always done. I finds that even when asked, most pathologists do not return poorly stained slides, yet how can we know of deficiencies if they do not? I worked in several histology labs over my career and slides were checked in all but two. I was able to introduce it in one of those, but not in the other due to technologist resistance (we've always done it that way). It was the technologists who resisted it. I don't see why Dr. Richmond needs to apologise. He merely drew attention to his work experiences in numerous histology labs and decried what is, in his experience, a common practice. In other words, he spoke about real, actual events. Why does being truthful require an apology? Are our egos so fragile that we can't take valid criticism as a profession nor as individuals? He certainly did not accuse those who got upset of failing to check slides, so why take umbrage? Noting and decrying poor histological practices should be a concern for everyone. Surely we are concerned about service to patients not about our egos. Bryan Llewellyn From kmerriam2003 <@t> yahoo.com Thu Jan 28 08:45:31 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Jan 28 08:45:35 2010 Subject: [Histonet] Fw: [IHCRG] Dianova rat anti-mouse CD31 In-Reply-To: <785BBF0C5F49CE41BA74460A43A08F0216650B6ED0@DCPWVMBXC0VS3.mdanderson.edu> References: <990075.43781.qm@web50308.mail.re2.yahoo.com> <785BBF0C5F49CE41BA74460A43A08F0216650B6ED0@DCPWVMBXC0VS3.mdanderson.edu> Message-ID: <434012.5249.qm@web50307.mail.re2.yahoo.com> Hi - someone emailed me about my methodology, but I cant find the email, so here it is: ? I tried it @ 1:10, 1:25 and 1:50.? All seem viable?dilutions for the?FFPE xenografts. I used BIocare DIVA (EDTA) HIER in decloaking chamber, primary?ab for 2 hours @ RT, Biocare Rat-on-Mouse HRP polymer?kit (15 minutes each) + DAB.??The path labs (for my company) that are in California and Seattle?used the same retreival but different detection methods and got similar results. Kim From:ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Kim Merriam Sent: Wednesday, January 27, 2010 9:23 AM To: Histonet; ihcrg@googlegroups.com Subject: [IHCRG] Dianova rat anti-mouse CD31 ? Hi All, ? I know there was some buzz a while back about this antibody.? I have tested it on mouse FFPE xenografts, and I must say, it looks very promising.? I compared 2?pieces of?the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was?fixed in NBF.? The NBF-fixed tumor actually looks better! ? A couple of other folks that are working at?the west coast sites of my company have had similar results.? We are all very encouraged. ? I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone. ? Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From JWatson <@t> gnf.org Thu Jan 28 09:18:32 2010 From: JWatson <@t> gnf.org (James Watson) Date: Thu Jan 28 09:18:39 2010 Subject: [Histonet] RE: [IHCRG] Dianova rat anti-mouse CD31 In-Reply-To: <434012.5249.qm@web50307.mail.re2.yahoo.com> References: <990075.43781.qm@web50308.mail.re2.yahoo.com> <785BBF0C5F49CE41BA74460A43A08F0216650B6ED0@DCPWVMBXC0VS3.mdanderson.edu> <434012.5249.qm@web50307.mail.re2.yahoo.com> Message-ID: I just got some of this antibody in an will be setting it up on the Ventana Discovery Thank you for the information on your protocol. I will let you know how it goes. Jamie James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Kim Merriam Sent: Thursday, January 28, 2010 6:46 AM To: Histonet; ihcrg@googlegroups.com Subject: Fw: [IHCRG] Dianova rat anti-mouse CD31 Hi - someone emailed me about my methodology, but I cant find the email, so here it is: I tried it @ 1:10, 1:25 and 1:50. All seem viable dilutions for the FFPE xenografts. I used BIocare DIVA (EDTA) HIER in decloaking chamber, primary ab for 2 hours @ RT, Biocare Rat-on-Mouse HRP polymer kit (15 minutes each) + DAB. The path labs (for my company) that are in California and Seattle used the same retreival but different detection methods and got similar results. Kim From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Kim Merriam Sent: Wednesday, January 27, 2010 9:23 AM To: Histonet; ihcrg@googlegroups.com Subject: [IHCRG] Dianova rat anti-mouse CD31 Hi All, I know there was some buzz a while back about this antibody. I have tested it on mouse FFPE xenografts, and I must say, it looks very promising. I compared 2 pieces of the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was fixed in NBF. The NBF-fixed tumor actually looks better! A couple of other folks that are working at the west coast sites of my company have had similar results. We are all very encouraged. I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From lilbullrider00 <@t> yahoo.com Thu Jan 28 09:34:11 2010 From: lilbullrider00 <@t> yahoo.com (brent hart) Date: Thu Jan 28 09:34:17 2010 Subject: [Histonet] Kp Marker Plus Slide Pen Message-ID: <413526.77013.qm@web53404.mail.re2.yahoo.com> Good morning fellow Histotechs, ? Does anyone know of a supplier for the KP Maker Plus slide pens? Any information on this would be very helpful thanks as I can not seem to locate a vendor. From leiker <@t> buffalo.edu Thu Jan 28 09:37:04 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Jan 28 09:37:12 2010 Subject: [Histonet] Kp Marker Plus Slide Pen In-Reply-To: <413526.77013.qm@web53404.mail.re2.yahoo.com> References: <413526.77013.qm@web53404.mail.re2.yahoo.com> Message-ID: <1818850FA5E8823FD04D24BF@CDYwxp1931.ad.med.buffalo.edu> 1st hit on Google: I forgot where I got ours from, but it was a different supplier, so there's more than 1 out there. Regards, Merced --On Thursday, January 28, 2010 7:34 AM -0800 brent hart wrote: > Good morning fellow Histotechs, > ? > Does anyone know of a supplier for the KP Maker Plus slide pens? Any > information on this would be very helpful thanks as I can not seem to > locate a vendor. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From lblazek <@t> digestivespecialists.com Thu Jan 28 09:39:29 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jan 28 09:39:59 2010 Subject: [Histonet] Kp Marker Plus Slide Pen In-Reply-To: <413526.77013.qm@web53404.mail.re2.yahoo.com> References: <413526.77013.qm@web53404.mail.re2.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390E976D90D9@IBMB7Exchange.digestivespecialists.com> Mercedes Medical -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of brent hart Sent: Thursday, January 28, 2010 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kp Marker Plus Slide Pen Good morning fellow Histotechs, ? Does anyone know of a supplier for the KP Maker Plus slide pens? Any information on this would be very helpful thanks as I can not seem to locate a vendor. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Thu Jan 28 09:56:55 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Jan 28 09:57:01 2010 Subject: [Histonet] Fwd: Re: Stains for Macrophages for Laser Capture Microdissection purpose Message-ID: ----- Original Message ----- From: Christopher Pin Date: Thursday, January 28, 2010 9:22 Subject: Re: Stains for Macrophages for Laser Capture Microdissection purpose To: Martin Sandig , John Kiernan , Paul Walton > Hi all, > > Thanks for including me in these discussions. I am no expert on macrophage histology but I know something about laser capture microdissection. The key to this method for RNA isolation is not so much in the timing but rather in the solutions complete lack of RNAse. We have worked with pancreatic tissue and been able to dissect of cells for RNA isolation. Likely one approach would be to do H&E for identification. Before you went to the trouble of doing LCM, you could take some unimportant tissue, scrape the tissue off the slide after fixation and then isolate RNA just to verify the integrity of the RNA following staining. > > The other thing that you can do is fix the slides as if you are doing in situ hybridization before you do any histology. > > I know this isn?t that helpful but from my experience it is not the time but rather the quality of the solutions that makes all the difference. > > Chris > > > On 1/28/10 9:01 AM, "Martin Sandig" wrote: > > Hi: > I do not do laser capture at all. > For identification purposes, perhaps it would be possible to load the macrophages with an injected dye (they eat that stuff depending on the tissue under investigation) and then capture them. In atherosclerotic lesions they are loaded with lipids (foam cells) and perhaps with an excellent microscope they may be visible before capture. > Chris Pin (cpin@uwo.ca) may have some experience with laser micro-dissection. > Cheers, > Martin > > > Martin Sandig, PhD > Associate Professor > Associate Chair, Undergraduate Affairs > Department of Anatomy and Cell Biology > Schulich School of Medicine and Dentistry > University of Western Ontario > Dental Sciences Building, Room 00075 > Phone: 519 661 2111 86815 > Fax: 519 850 25662 > http://www.uwo.ca/anatomy/department/sandigm/msandig.html > > > > >>> Paul Walton 28/01/2010 8:24 am >>> > John, > I have forwarded this message on to Martin, who does laser capture. Alas, I am the microinjection fellow. > Paul > > Begin forwarded message: > > From: John Kiernan > Date: January 27, 2010 10:41:15 PM EST (CA) > To: delphine eberle > Cc: sharon.willman@bms.com, histonet@lists.utsouthwestern.edu, pwalton@uwo.ca > Subject: Re: Stains for Macrophages for Laser Capture Microdissection purpose > > How about phase contrast optics and identifying the cells by their shapes? Non-specific addition of colour to a section of unfixed tissue probably will not show the cells any more clearly. I have taken the liberty of including a colleague, Dr Paul Walton, in this reply. He does laser capture microdissection and may have something to suggest. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: delphine eberle > Date: Wednesday, January 27, 2010 16:27 > Subject: Stains for Macrophages for Laser Capture Microdissection purpose > To: jkiernan@uwo.ca, sharon.willman@bms.com > Cc: histonet@lists.utsouthwestern.edu > > Hi, > > > > I have another question following Macrophage staining. > > I am setting up a Laser Capture Microdissection protocol to dissect macrophages from atherosclerosis lesions (non fixed frozen sections) and extract RNA for gene expression purpose. > > I am looking at a quick staining (less than 30min otherwise RNA is degradated) for macrophages in that context? Any suggestions? > > > > Thanks a lot, > > Delphine > > > > Delphine Eberl? PhD > > UCSF Department of Vascular Surgery > > eberledelphine@hotmail.com > > > > > From: jkiernan@uwo.ca > > > To: sharon.willman@bms.com > > > Date: Wed, 27 Jan 2010 00:22:53 -0500 > > > Subject: Re: [Histonet] Histology Special Stains for Macrophages > > > CC: histonet@lists.utsouthwestern.edu > > > > > > None of the methods mentioned in the enquiry are stains for macrophages. Research workers who never took Histology 101 often stain cells of the monocyte/macrophage lineage immunohistochemically (IHC), using very expensive primary antibodies and fairly expensive kits to amplify and detect the binding sites. IHC is necessary if you must find every macrophage, including a tissue's recent monocyte immigrants that haven't yet done any work. Macrophages that have been busily eating blood are full of brown granules that don't need any staining. > > > > > > Ordinary people recognize macrophages by their appearance in sections stained with a well done H&E or with one of the many Romanowsky-Giemsa blood stains. With the latter it's important to get the pH right - much lower for sections of formaldehyde-fixed objects (pH4 to 5) than for methanol-fixed films or smears (traditionally 6.8). It's all very well explained in RD Lillie & GM Fullmer's "Histopathologic Technic..." (4th ed 1976, pp.193-197; the 3rd edition, 1965, is OK too) and also in more recent textbooks including those by Bancroft & Gamble, Geoffrey Brown, Freida Carson and, of course > > > Yours truly. > > > > > > John Kiernan > > > Anatomy, UWO > > > London, Canada > > > http://publish.uwo.ca/~jkiernan/bookfind.htm > > > = = = > > > ----- Original Message ----- > > > From: "Willman, Sharon" > > > Date: Tuesday, January 26, 2010 12:12 > > > Subject: [Histonet] Histology Special Stains for Macrophages > > > To: "histonet@lists.utsouthwestern.edu" > > > > > > > Hi, > > > > We are needing to do a special stain for macrophages. What > > > > is the most common stain for that? Does anyone do a Sudan > > > > Black, Alcian Blue or Van Gieson for macrophages? Any > > > > information would be appreciated. > > > > Thanks in advance. > > > > Sharon > > > > > > > > > > > > ________________________________ > > > > This message (including any attachments) may contain > > > > confidential, proprietary, privileged and/or private > > > > information. The information is intended to be for the use of > > > > the individual or entity designated above. If you are not the > > > > intended recipient of this message, please notify the sender > > > > immediately, and delete the message and any attachments. Any > > > > disclosure, reproduction, distribution or other use of this > > > > message or any attachments by an individual or entity other than > > > > the intended recipient is prohibited. > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Vous cherchez l'int?grale des clips de Michael Jackson ? Bing ! Trouvez ! > > Paul Albert Walton, Ph.D. > Department of Anatomy and Cell Biology > #474 Medical Sciences Building > The University of Western Ontario > London, Ontario, CANADA N6A 5C1 > (519) 661-2111 x86825 > fax (519) 661-3936 > ------------------------- > > > > > > > > Christopher Pin, Ph.D. > Associate Professor > Depts. Of Paediatrics and Physiology & > Pharmacology > Schulich School of Medicine and Dentistry > University of Western Ontario > Scientist, Children's Health Research Institute From leiker <@t> buffalo.edu Thu Jan 28 10:13:58 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Jan 28 10:14:03 2010 Subject: [Histonet] Kp Marker Plus Slide Pen In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390E976D90D9@IBMB7Exchange.digestivespecialists.com> References: <413526.77013.qm@web53404.mail.re2.yahoo.com> <5A2BD13465E061429D6455C8D6B40E390E976D90D9@IBMB7Exchange.digestivespecialists.com> Message-ID: <113FCB366CA3DE24468A7C0F@CDYwxp1931.ad.med.buffalo.edu> Yeah, that's it - that's where we got ours from. Plus the Mercedes Medical rep called to remind me after seeing my post, so he does monitor the Histonet... ;-) --On Thursday, January 28, 2010 10:39 AM -0500 "Blazek, Linda" wrote: > Mercedes Medical > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of brent hart > Sent: Thursday, January 28, 2010 10:34 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Kp Marker Plus Slide Pen > > Good morning fellow Histotechs, > ? > Does anyone know of a supplier for the KP Maker Plus slide pens? Any > information on this would be very helpful thanks as I can not seem to > locate a vendor. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From jfish <@t> gladstone.ucsf.edu Thu Jan 28 10:23:05 2010 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Thu Jan 28 10:23:14 2010 Subject: [HISTONET] embedding cell cultures In-Reply-To: <4B609FD8.7030303@umdnj.edu> References: <1776573639.2479441264616742541.JavaMail.root@zm06.stanford.edu> <4B609FD8.7030303@umdnj.edu> Message-ID: Hi Nick, If you can, grow the cells on thermanox cover slips (NUNC). Then process them as normal for embedding in Eponate 12. I use acetonitrile in place of the propylene oxide to avoid damaging the coverslip. When doing the final embedding and polymerization, invert the coverslip over a drop of resin on a square of ACLAR sheeting. This forms a "sandwich" of your cell layer. After polymerization you separte the resin layer from the ACLAR and the coverslip (very easy! No sawing!) and "re-embed" one or more layers on their side in a resin block. Now, section away. You will get beautiful sections of your cells at a nice 90 degree angle. The best part of doing it this way is that you can put the embedded cell layer in the light microscope and select any area of interest before re-embedding and sectioning. Good luck! Jo Dee ~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 Nicholas David Evans wrote: > Dear all, > > I was hoping someone might be able to offer me some advice on embedding and sectioning cell cultures. > > In short we are growing cells which form 3D dome-like structures on tissue culture plastic. Does anyone have any experience or advice to offer on embedding the cultures in situ before sectioning? I have seen various methods in the literature, which often use Epon to embed the material followed by sawing away the plastic, but if anyone can offer some tips on other possible (easier) ways of doing it, or can refer me to some useful literature, I'd be very grateful. > > I would like to have simple 10um sections at 90 degrees to the substrate, which I can use for IHC. > > Best wishes > Nick > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Thu Jan 28 10:55:39 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Jan 28 10:58:37 2010 Subject: [Histonet] Kp Marker Plus Slide Pen In-Reply-To: <113FCB366CA3DE24468A7C0F@CDYwxp1931.ad.med.buffalo.edu> References: <413526.77013.qm@web53404.mail.re2.yahoo.com> <5A2BD13465E061429D6455C8D6B40E390E976D90D9@IBMB7Exchange.digestivespecialists.com> <113FCB366CA3DE24468A7C0F@CDYwxp1931.ad.med.buffalo.edu> Message-ID: These are the BEST pens I have ever bought for Histology! They last way longer plus the writing is very black and the colour stays... even when you reprocess a block!!!! Yes... I mark my kids school supplies with them too! Mercedes Medical 1-800-331-2716 www.MercedesMedical.com These are all I ever buy anymore... no one comes close. Maria Katleba HT (ASCP) MS Queen of the Valley Medical Center Napa CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Thursday, January 28, 2010 8:14 AM To: Blazek, Linda; 'brent hart'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kp Marker Plus Slide Pen Yeah, that's it - that's where we got ours from. Plus the Mercedes Medical rep called to remind me after seeing my post, so he does monitor the Histonet... ;-) --On Thursday, January 28, 2010 10:39 AM -0500 "Blazek, Linda" wrote: > Mercedes Medical > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of brent hart > Sent: Thursday, January 28, 2010 10:34 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Kp Marker Plus Slide Pen > > Good morning fellow Histotechs, > > Does anyone know of a supplier for the KP Maker Plus slide pens? Any > information on this would be very helpful thanks as I can not seem to > locate a vendor. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From lblazek <@t> digestivespecialists.com Thu Jan 28 11:00:15 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jan 28 11:00:45 2010 Subject: [Histonet] Kp Marker Plus Slide Pen In-Reply-To: References: <413526.77013.qm@web53404.mail.re2.yahoo.com> <5A2BD13465E061429D6455C8D6B40E390E976D90D9@IBMB7Exchange.digestivespecialists.com> <113FCB366CA3DE24468A7C0F@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E390E976D90DE@IBMB7Exchange.digestivespecialists.com> They last so long! That's why it's easy to forget where they came from! -----Original Message----- From: Maria Katleba [mailto:Maria.Katleba@stjoe.org] Sent: Thursday, January 28, 2010 11:56 AM To: Merced M Leiker; Blazek, Linda; 'brent hart'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kp Marker Plus Slide Pen These are the BEST pens I have ever bought for Histology! They last way longer plus the writing is very black and the colour stays... even when you reprocess a block!!!! Yes... I mark my kids school supplies with them too! Mercedes Medical 1-800-331-2716 www.MercedesMedical.com These are all I ever buy anymore... no one comes close. Maria Katleba HT (ASCP) MS Queen of the Valley Medical Center Napa CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Thursday, January 28, 2010 8:14 AM To: Blazek, Linda; 'brent hart'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kp Marker Plus Slide Pen Yeah, that's it - that's where we got ours from. Plus the Mercedes Medical rep called to remind me after seeing my post, so he does monitor the Histonet... ;-) --On Thursday, January 28, 2010 10:39 AM -0500 "Blazek, Linda" wrote: > Mercedes Medical > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of brent hart > Sent: Thursday, January 28, 2010 10:34 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Kp Marker Plus Slide Pen > > Good morning fellow Histotechs, > > Does anyone know of a supplier for the KP Maker Plus slide pens? Any > information on this would be very helpful thanks as I can not seem to > locate a vendor. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From kryan <@t> nfderm.com Thu Jan 28 11:17:50 2010 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Thu Jan 28 11:17:55 2010 Subject: [Histonet] I am no longer receiving messages from the histonet Message-ID: Kaye Ryan North Florida Dermatology Associates Histology Lab Supervisor (904) 398-0547 Ext. 2004 From pruegg <@t> ihctech.net Thu Jan 28 11:39:14 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Jan 28 11:40:31 2010 Subject: [Histonet] RE: [IHCRG] Dianova rat anti-mouse CD31 In-Reply-To: References: <990075.43781.qm@web50308.mail.re2.yahoo.com> <785BBF0C5F49CE41BA74460A43A08F0216650B6ED0@DCPWVMBXC0VS3.mdanderson.edu> <434012.5249.qm@web50307.mail.re2.yahoo.com> Message-ID: <5B29573BBC734F1FB7BBD2E040FA6A4F@Patsyoffice> Thank you Kim, that does sound very promising, I still have not gotten around to trying my sample of this, your work will be very helpful when I do. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of James Watson Sent: Thursday, January 28, 2010 8:19 AM To: 'kmerriam2003@yahoo.com'; Histonet; ihcrg@googlegroups.com Subject: RE: [IHCRG] Dianova rat anti-mouse CD31 I just got some of this antibody in an will be setting it up on the Ventana Discovery Thank you for the information on your protocol. I will let you know how it goes. Jamie James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Kim Merriam Sent: Thursday, January 28, 2010 6:46 AM To: Histonet; ihcrg@googlegroups.com Subject: Fw: [IHCRG] Dianova rat anti-mouse CD31 Hi - someone emailed me about my methodology, but I cant find the email, so here it is: I tried it @ 1:10, 1:25 and 1:50. All seem viable dilutions for the FFPE xenografts. I used BIocare DIVA (EDTA) HIER in decloaking chamber, primary ab for 2 hours @ RT, Biocare Rat-on-Mouse HRP polymer kit (15 minutes each) + DAB. The path labs (for my company) that are in California and Seattle used the same retreival but different detection methods and got similar results. Kim From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Kim Merriam Sent: Wednesday, January 27, 2010 9:23 AM To: Histonet; ihcrg@googlegroups.com Subject: [IHCRG] Dianova rat anti-mouse CD31 Hi All, I know there was some buzz a while back about this antibody. I have tested it on mouse FFPE xenografts, and I must say, it looks very promising. I compared 2 pieces of the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was fixed in NBF. The NBF-fixed tumor actually looks better! A couple of other folks that are working at the west coast sites of my company have had similar results. We are all very encouraged. I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From POWELL_SA <@t> mercer.edu Thu Jan 28 12:35:48 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Jan 28 12:35:56 2010 Subject: [Histonet] Ga.Society For Histotechnology Reservation Link Message-ID: <9BF995BC0E47744E9673A41486E24EE22429F09B78@MERCERMAIL.MercerU.local> Hi All, I wanted to pass this link from the hotel on to you guys who will be attending the Georgia Society for Histotechnology meeting March 26-28 this year. This goes straight to the hotel and already has our code entered for you to reserve your rooms at the Evergreen Marriott Conference Resort. For the complete program and registration form go to www.histosearch.com/gsh and go to the symposium page. You will find the hotel link there too. See you there. Shirley Powell GSH Secretary Subject: Ga.Society For Histotechnology Reservation Link Simply cut and paste the link below and include with your electronic correspondence to facilitate the reservation process. Your guests will be directed to the property's home page with the code already entered in the appropriate field. All they need to do is enter their arrival date to begin the reservation process. Evergreen Marriott Conference Resort >> [http://www.marriott.com/Images/Brands/MCC/Logos/MCC_logowhitefield_120x60.gif] http://www.marriott.com/hotels/travel/atleg?groupCode=gshgsha&app=resvlink&fromDate=3/26/10&toDate=3/27/10 From rlhenshall_powell <@t> yahoo.co.uk Thu Jan 28 13:08:33 2010 From: rlhenshall_powell <@t> yahoo.co.uk (Rhonda Henshall-Powell) Date: Thu Jan 28 13:08:38 2010 Subject: [Histonet] Re: Histonet Digest, Vol 74, Issue 34 Message-ID: <828544.67370.qm@web23205.mail.ird.yahoo.com> Dear Nick, When I was growing and harvesting 3D cell cultures (spheroids grown in matrigel) we would remove the culture medium, draw up the gel in a needle-less syringe and place in to OCT embedding medium (Tissue Tek) before freezing in Liquid Nitrogen. I would then cut 5-10um sections and perform IHC or immunofluorescence. Hope this helps - but it looks like you have a lot of good suggestions already. Best Regards, Rhonda Rhonda Henshall-Powell, Ph.D. > > ------------------------------ > > Message: 2 > Date: Wed, 27 Jan 2010 10:25:42 -0800 (PST) > From: Nicholas David Evans > > Dear all, > > I was hoping someone might be able to offer me some advice > on embedding and sectioning cell cultures. > > In short we are growing cells which form 3D dome-like > structures on tissue culture plastic. Does anyone have any > experience or advice to offer on embedding the cultures in > situ before sectioning? I have seen various methods in the > literature, which often use Epon to embed the material > followed by sawing away the plastic, but if anyone can offer > some tips on other possible (easier) ways of doing it, or > can refer me to some useful literature, I'd be very > grateful. > > I would like to have simple 10um sections at 90 degrees to > the substrate, which I can use for IHC. > > Best wishes > Nick > > > > ------------------------------ > > Message: 3 > Date: Wed, 27 Jan 2010 15:19:36 -0500 > From: Geoff McAuliffe > > Hi Nick: > > You can use the Epon substitutes such as EmBed 812. Fix, > osmicate, > dehydrate as usual, but omit the proplylene oxide as it > will react with > the plastic dish. Epon substitutes will mix with ethanol. I > used 2:1 > then 1:1 then 1:2 ratios of absolute ethanol to "Epon" with > catalyst > added with agitation. Then several changes of pure Epon and > polymerize. > Yes, you will have to saw away the plastic, if you try to > section the > Epon-plastic combo the two will separate. > How much easier do you want it to be? > > Geoff > ------------------------------ > > Message: 5 > Date: Wed, 27 Jan 2010 15:39:05 -0500 > From: "Sherwood, Margaret " > Subject: RE: [HISTONET] embedding cell cultures > Nick, > > I am assuming that your 3D cells only grow on > plastic.? They make plastic cover > slips which, if your cells attach to them and grow, might > make the embedding > much easier.? You would follow the same method as > stated, but then you could > invert the coverslips on a beem capsule and separate the > coverslip from capsule > with liquid nitrogen.? However, I have never done it > with plastic coverslips > (only glass), so not sure if they would easily separate > from capsule with liquid > nitrogen. If anyone else has done so, please weigh in. > > Peggy??? > > ------------------------------ > Message: 6 > Date: Wed, 27 Jan 2010 15:44:37 -0500 > From: Peggy Bisher > Subject: Re: [HISTONET] embedding cell cultures > > I have done just what you are talking about using Aclar. It > is a plastic > embedding film (purchased from EMS). It works great for > us. > > Margaret E. Bisher > Electron Microscopy & Histology Core Facility Manager > Department of Molecular Biology > Princeton University > Moffett Laboratory, Room 113 > Princeton, New Jersey > Office: (609) 258-7026 > Fax: (609) 258-8468 > mbisher@princeton.edu From Margaret.Perry <@t> sdstate.edu Thu Jan 28 15:00:12 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Jan 28 15:00:25 2010 Subject: [Histonet] need help with stain In-Reply-To: <20100128174233.B79C12174D7@barracuda.sdstate.edu> References: <20100128174233.B79C12174D7@barracuda.sdstate.edu> Message-ID: A researcher wants to measure the length of the intestinal crypts and asked for a suggestion on what type of stain to use. I am thinking of using a phloxine/tartrazine stain and do a double stain with a GMS to show the basement membrane. What is your opinion on this? Do you have other suggestions that would work better? Hope you are warmer than here in SD! Thanks for the help. Margaret Perry South Dakota State University From rjbuesa <@t> yahoo.com Thu Jan 28 15:21:17 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 28 15:21:22 2010 Subject: [Histonet] need help with stain In-Reply-To: Message-ID: <877399.36977.qm@web65714.mail.ac4.yahoo.com> Use mucicarmin stain. The crypt mucus will be evident and? their sizes. Ren? J. --- On Thu, 1/28/10, Perry, Margaret wrote: From: Perry, Margaret Subject: [Histonet] need help with stain To: "histonet@lists.utsouthwestern.edu" Date: Thursday, January 28, 2010, 4:00 PM A researcher wants to measure the length of the intestinal crypts and asked for a suggestion on what type of stain to use.? I am thinking of using a phloxine/tartrazine stain and do a double stain with a GMS to show the basement membrane.? What is your opinion on this? Do you have other suggestions that would work better? Hope you are warmer than here in SD! Thanks for the help. Margaret Perry South Dakota State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thomas.crowell <@t> novartis.com Thu Jan 28 15:24:40 2010 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Thu Jan 28 15:26:49 2010 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 01/28/2010 and will not return until 02/02/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From JMcCormick <@t> schosp.org Thu Jan 28 15:29:09 2010 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Thu Jan 28 15:29:16 2010 Subject: [SPAM-HC] - [Histonet] Microtomist's Vade-Mecum on the Web - Email found in subject In-Reply-To: References: Message-ID: <0CA58D7DD405854899D129A03276B1334439313EA3@EXCHCCRMB.schosp.org> Samuri......... The website www.scienceheritagelimited.com has available 8 books that are reprints of historically important "history of microscopy and histotechnology books" included is the 1885 edition of Arthur Bolles Lee's "The Microtomist's Vade-Mecum". It's amazing how little (relatively) things have changed. The only thing missing in the 1885 edition is the use of formalin as a tissue fixative. This did not come about until 1892 and undoubtedly appears in his later editions. Good reading, jim J.B.McCormick, M.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, January 28, 2010 7:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] Microtomist's Vade-Mecum on the Web - Email found in subject Arthur Bolles Lee's The Microtomist's Vade-Mecum, 7th edition (1913) is available online on Google Books, along with some other editions: http://books.google.com/books?id=4m5MAAAAMAAJ&printsec=frontcover&dq=arthur+bolles+lee&ei=BpJhS96xKJTIzASV9vnNBQ&cd=1#v=onepage&q=&f=false This marvelous old histology recipe book was a great rarity before the Web, though it's actually now available from Amazon. It took me several years to find a copy in 1976 - found one in a consignment of old books discarded from the library of a long-defunct college. A note about the title: Vade-Mecum (pronounced something like voddy-make-um) - literally "come with me" (think of "Quo Vadis) is an old word for a practical handbook of something. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From AnthonyH <@t> chw.edu.au Thu Jan 28 16:21:55 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jan 28 16:22:07 2010 Subject: [Histonet] Re: Diff-Quik In-Reply-To: Message-ID: Please don't take it personally. If we are trying our best to meet our own high expectations then we do not have to worry. I believe that most of us do what Robert expects. This is supported by my experiences at the recent NSH meeting in Birmingham Alabama. I was privileged to meet some of the most dedicated professionals around and believe that our profession is heading in the right direction. Robert's comments should be heeded and pondered. I definitely did. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Thursday, 28 January 2010 2:53 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Diff-Quik I'm going to have to agree with Cheryl on the comment. This may be your experience but I can tell you my techs always look at their stains before they send it on to the Pathologist. It is a requirement that they understand what they are looking at in order to know if it worked. Each of them are also trained to know all tissues microscopically and all stain components microscopically. That is after all the purpose of being a Histologist. I am going out on a limb here and I normally don't, but you are digging yourself in to a rather rude hole to insult so many professional Histologist. Just saying............. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 01/26/2010 07:38 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Re: Diff-Quik I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before. I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope. The more quality assurance paperwork I have to do, the worse the slides. The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services. Did Edwards Deming live in vain? Bob Richmond Samurai Pathologist Knoxville TN ************************************* On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller wrote: > Every slide I stain, special stains, IHC or otherwise I check under > the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Monday, January 25, 2010 7:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Diff-Quik > > Thanks, John Kiernan, for your explanation of Romanovsky stains. > > "Diff-Quik" (please note the spelling) is the trademarked name of a > staining sequence consisting of a fixative, eosin (Diff-Quik I), and > an azure (Diff-Quik II), done in that order in three separate > containers. I'm not sure who the trademark presently belongs to - it > seems to change with the phases of the Moon. > > There are a number of generic equivalents, which in my personal > experience all work as well as trademark Diff-Quik. For most ordinary > pathology services, it isn't worthwhile to try to brew your own. > > I don't think I've seen bone marrow stained with such a sequence. > Proper staining of bone marrows requires that the histotechnologist > examine the slides under a microscope, a practice too many find > abhorrent. > > Bob Richmond > Samurai Pathologist > Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From pathmaster <@t> yahoo.com Thu Jan 28 16:53:25 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Thu Jan 28 16:53:28 2010 Subject: [Histonet] A blast from the past.... Message-ID: <276280.46298.qm@web111112.mail.gq1.yahoo.com> Dr Richmond, ? Thanks so much for that link to Vade Mecum. I've never before seen the book, but it has brought me back to my childhood, when I found a similar histotechnique book, ?Michael Guyer's Animal Micrology,?in an antique shop my mom was browsing, some 44 years ago. This book was referenced all over that book, and in Gray's Microtomist's Formulary and Guide which I obtained later as my interest in histotechnique grew during high school, an interest kindled by that book? and resulting in a rewarding and fascinating career. I feel like a kid again. Very cool. ? It's amazing how much mercury they slung around back then. Hell, I made my own Zenker's fluid in high school from those books, bet the waste went down the drain too. ? Jeff Silverman? From greenjumpyone <@t> hotmail.com Thu Jan 28 18:38:59 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Thu Jan 28 18:39:03 2010 Subject: [Histonet] (no subject) Message-ID: http://www.nesle2005.republika.pl/T6vJjAE12x.html _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/196390707/direct/01/ From JMacDonald <@t> mtsac.edu Fri Jan 29 00:03:34 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Jan 29 00:03:40 2010 Subject: [Histonet] honing compound Message-ID: A colleague from a neighboring university is trying to find both fine and coarse honing compound for his knife sharpener. Does anyone have a good source? Thank you, Jennifer MacDonald From mpence <@t> grhs.net Fri Jan 29 08:13:30 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Jan 29 08:13:35 2010 Subject: [Histonet] A blast from the past.... In-Reply-To: <276280.46298.qm@web111112.mail.gq1.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D28@is-e2k3.grhs.net> How true!.... Can you believe the things we use to put down the drain or in the trash? "Out of sight, out of mind". Today a high chemistry lab would be shut down and the teacher sent to jail for some of the things they taught us back then. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Thursday, January 28, 2010 4:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A blast from the past.... Dr Richmond, ? Thanks so much for that link to Vade Mecum. I've never before seen the book, but it has brought me back to my childhood, when I found a similar histotechnique book, ?Michael Guyer's Animal Micrology,?in an antique shop my mom was browsing, some 44 years ago. This book was referenced all over that book, and in Gray's Microtomist's Formulary and Guide which I obtained later as my interest in histotechnique grew during high school, an interest kindled by that book? and resulting in a rewarding and fascinating career. I feel like a kid again. Very cool. ? It's amazing how much mercury they slung around back then. Hell, I made my own Zenker's fluid in high school from those books, bet the waste went down the drain too. ? Jeff Silverman? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vygutz <@t> yahoo.com Fri Jan 29 08:41:34 2010 From: vygutz <@t> yahoo.com (Vanessa Gutierrez) Date: Fri Jan 29 08:41:38 2010 Subject: [Histonet] Re : KP Markers Message-ID: <738912.31896.qm@web30206.mail.mud.yahoo.com> KP?Marker pens are sold?by Mercedes Medical. I use them and I really like them.?The?box I ordered is still on back order so you want to order as soon as possible. From Vickroy.Jim <@t> mhsil.com Fri Jan 29 09:21:49 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Jan 29 09:21:53 2010 Subject: [Histonet] b-5 Message-ID: <24A4826E8EF0964D86BC5317306F58A54256B2E3FA@mmc-mail.ad.mhsil.com> Someone remind me. (senior moment). Years ago we stopped using B-5 fixative in our lab. If I remember right we were given a date by CAP to either stop using a mercury product or present a plan on when we would stop using it. Am I correct and if not are some people still using B-5? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From sbreeden <@t> nmda.nmsu.edu Fri Jan 29 09:37:24 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jan 29 09:37:29 2010 Subject: [Histonet] Friday Hour of Fun Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46E12@nmdamailsvr.nmda.ad.nmsu.edu> I have a Hypothetical Situation for which I would like your input. If you - as a histologist - were to be involved in the interview of a potential staff pathologist, what questions would you ask this candidate? Think of the practical and logical issues, but also the unique and pertinent secondary Things You'd Like to Know. I would like input from techs AND pathologists. If you were a pathologist in an interview situation, what questions would you think appropriate? If you're a tech, what would you like to know about this candidate before he begins to orbit your universe? Please write me off Histonet - not for any other reason than it's likely that everyone will not be interested in this subject. Thank you again and I can't wait to see what answers I will get! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From JWeems <@t> sjha.org Fri Jan 29 09:42:08 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Jan 29 09:42:13 2010 Subject: [Histonet] b-5 In-Reply-To: <24A4826E8EF0964D86BC5317306F58A54256B2E3FA@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A54256B2E3FA@mmc-mail.ad.mhsil.com> Message-ID: Shhhhhhhhhhhhhhhhh, if I understand correctly, they are not legal. It was several years ago that we were mandated to stop. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Friday, January 29, 2010 10:22 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] b-5 Someone remind me. (senior moment). Years ago we stopped using B-5 fixative in our lab. If I remember right we were given a date by CAP to either stop using a mercury product or present a plan on when we would stop using it. Am I correct and if not are some people still using B-5? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From kblack <@t> digestivehlth.com Fri Jan 29 10:04:28 2010 From: kblack <@t> digestivehlth.com (Konni Black) Date: Fri Jan 29 10:04:36 2010 Subject: [Histonet] job opening Message-ID: We are looking for a fulltime histologist for a new lab in Olympia, WA. Planned opening in March with great new equipment and lab space. The ideal candidate will have 3 to 5 years experience and must be able to work alone. Flexible work schedule. . Contact Konni Black kblack@digestivehlth.com 253-503-2560 From Sharon.Davis-Devine <@t> carle.com Fri Jan 29 10:16:16 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Fri Jan 29 10:16:21 2010 Subject: [Histonet] Decontamination of a Cryostat Message-ID: <50003EC02E2CEA4583BEB3CD08EAC1E090DDD5@EXCHANGEBE2.carle.com> This issue seems to be rearing its ugly head in our lab once again. What is the correct procedure for decontaminating a cryostat after let's say a specimen from an HIV patient for a frozen was cut on it? We have a couple of different cryostats, one of them defrosts quickly and the other one ices over and defrosts very slowly. Our PA's assist with 98% of the frozens and will use either one of the cryostats. Our pathologists, who perform frozens by themselves on a rare occasion, prefer the cryostat that defrosts very slowly. Of course the cryostat that was contaminated was down awaiting decontamination when a pathologist had to perform a frozen. We are now being told that we need to replace the cryostat that the pathologist doesn't like to use. So have any of you had any issues like this and if you have how did you handle it? Are there any decontamination procedures available for a quick turnaround? Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From Jan.Minshew <@t> leica-microsystems.com Fri Jan 29 10:34:29 2010 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Fri Jan 29 10:34:48 2010 Subject: [Histonet] Decontamination of a Cryostat In-Reply-To: <50003EC02E2CEA4583BEB3CD08EAC1E090DDD5@EXCHANGEBE2.carle.com> Message-ID: Hi Sharon, I copied and pasted a chemical disinfection procedure for cryostats that I created for Leica's sales and service employees and customers. It references Leica cryostats but it can be used for any cryostat--the basic principles for chemical disinfection are the same. Since we can't put attachments on the histonet, I am hoping that you will be able to read the copy without problem. If any of you have trouble, please let me know and I can send it to you off line as an attachment. Chemical Disinfection of a Cryostat According to CAP regulations, a cryostat should be defrosted and decontaminated with a tuberculocidal disinfectant at a time interval appropriate for the institution (once a week for instruments used daily). The regulations also state that the cryostat must be clearly marked as contaminated if a frozen section is performed on tissue from a patient known or suspected to be positive for HIV, hepatitis B or C, SARS-related coronavirus, prion disease such as Creutzfeldt-Jakob disease, or mycobacterial or systemic fungal disease. It must then be decontaminated before further use. ? Wear Personal Protective Equipment (PPE): Personal Protective Equipment, such as gowns, puncture and penetration resistant gloves and eye protection must be worn when performing cryostat disinfection procedures. ? Cryostat Preparation Remove used blades/knives from their holder. Although not a requirement, steel mesh gloves should be worn when changing knife blades. Dispose of blades according to the regulations of the institution or disinfect knives before reusing by soaking in disinfecting solution. Remove all debris and utensils (pencils, forceps, brushes, gauze, etc) from the chamber. Debris must be removed because organic material (blood and proteins) may contain high concentrations of microorganisms and could possibly inactivate the chemical disinfectant or prevent access to contaminated surfaces. It should be treated as biohazardous waste and disposed of according to the policies and procedures of the institution. 70% ethyl or reagent alcohol can be used to clean the cryostat and provide some disinfection capabilities. The germicidal activity of ethyl alcohol is most effective in the 70% range because it can penetrate tubercle bacteria and it has an advantage over isopropyl alcohol of being able to kill hydrophilic viruses. ? Chemical Disinfection In order to disinfect a cryostat using a chemical disinfectant, the instrument MUST be at room temperature before the process is started. Turn off and unplug the instrument before beginning the disinfection process. (Once the cryostat has reached room temperature, do not turn the handwheel until it has been returned to cold temperature.) Chemical Disinfection of a Cryostat continued: Do not create aerosols by spraying disinfectant (or anything else) in an open cryostat chamber. Pour disinfectants onto surfaces or absorbent disposable towels and allow them to remain in contact with contaminated surfaces for the length of time specified in the instructions of the individual agents. Use a tuberculocidal disinfectant that is non-corrosive. The EPA maintains a list of Antimicrobial Chemical/ Registration Number Indexes on their website, http://www.epa.gov/oppad001/chemregindex.htm), and it is updated regularly. From this link you can find agents effective against bloodborne pathogens such as Mycobacterium tuberculosis, human HIV-1 virus, and Hepatitis B or Hepatitis C virus. It is critical to remember that NONE of these solutions have been tested at low temperatures and can only be used at room temperature. Any disposable material used in the disinfection process must be disposed of in accordance with the policies and procedures of the institution. ? Following Disinfection After the disinfection procedure is complete, the cryostat and all of the accessories must be thoroughly dried and lubricated before being put back into service at cold temperatures. Absolute ethyl alcohol can be used to remove excess moisture from surfaces. Accessories with multiple parts, such as the disposable blade holder, knife holder and their respective bases, must be taken apart and dried thoroughly. Plug the instrument back in and turn it on. Use only the lubricants that are recommended by the manufacturer and only in the recommended amounts. Lightly lubricate any moving parts. To lubricate the specimen arm, extend it all the way forward. Apply ONE DROP of lubricant to the barrel and spread it around with your gloved finger. Move the arm back to the home position. The liquid waste container should be emptied in accordance with the policies and procedures of the institution. Before replacing in the instrument, add a small amount of liquid bleach to the empty container. For optimum sectioning, allow the instrument to cool long enough to allow the metal microtome parts to reach the cold temperature setting. The CM1850 requires no less than 3 hours and the CM1950 needs 5 hours to cool down from ambient temperature (20?C) to -25?C and 8 hours to cool from 20?C to -35?C. Kind regards, Jan Minshew Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 Office: 847.405.7051 Cell: 847.970.8468 Fax: 847.405.6560 www.leica-microsystems.com Click Here for this month's special offers! http://www.leica-microsystems.com/bsdspecial "Sharon.Davis-Dev ine" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Decontamination of a Cryostat 01/29/2010 10:16 AM This issue seems to be rearing its ugly head in our lab once again. What is the correct procedure for decontaminating a cryostat after let's say a specimen from an HIV patient for a frozen was cut on it? We have a couple of different cryostats, one of them defrosts quickly and the other one ices over and defrosts very slowly. Our PA's assist with 98% of the frozens and will use either one of the cryostats. Our pathologists, who perform frozens by themselves on a rare occasion, prefer the cryostat that defrosts very slowly. Of course the cryostat that was contaminated was down awaiting decontamination when a pathologist had to perform a frozen. We are now being told that we need to replace the cryostat that the pathologist doesn't like to use. So have any of you had any issues like this and if you have how did you handle it? Are there any decontamination procedures available for a quick turnaround? Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Janice.Mahoney <@t> alegent.org Fri Jan 29 10:44:04 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Jan 29 10:45:08 2010 Subject: [Histonet] RE: Friday Hour of Fun In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46E12@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46E12@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A562C@EXCHMBC2.ad.ah.local> I'd ask how he sees his relationship with the histotechnologists, histo supervisor, etc. I'd ask him what his expectation is regarding TAT of slides/special stains/IHC/rectus. If there is more than one Pathologist, I'd ask how they would collaborate to decide on standard work. For example, will you cut one two or three slides on GI bxs. (in other words will he accept that you will not do specific things for individual Pathologists, that they will decide on standard work). these are a few thing off the top of my head. Good luck, it will be interesting to see what others come up with. Jan Mahoney Omaha, N?E -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, January 29, 2010 9:37 AM To: histonet Subject: [Histonet] Friday Hour of Fun I have a Hypothetical Situation for which I would like your input. If you - as a histologist - were to be involved in the interview of a potential staff pathologist, what questions would you ask this candidate? Think of the practical and logical issues, but also the unique and pertinent secondary Things You'd Like to Know. I would like input from techs AND pathologists. If you were a pathologist in an interview situation, what questions would you think appropriate? If you're a tech, what would you like to know about this candidate before he begins to orbit your universe? Please write me off Histonet - not for any other reason than it's likely that everyone will not be interested in this subject. Thank you again and I can't wait to see what answers I will get! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Ronald.Houston <@t> nationwidechildrens.org Fri Jan 29 10:45:00 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Jan 29 10:45:42 2010 Subject: [Histonet] Kaposi's sarcoma block Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B646032@chi2k3ms01.columbuschildrens.net> Can anyone spare a block of Kaposi's sarcoma? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Timothy.Morken <@t> ucsfmedctr.org Fri Jan 29 10:45:45 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Jan 29 10:46:05 2010 Subject: [Histonet] RE: Decontamination of a Cryostat In-Reply-To: <50003EC02E2CEA4583BEB3CD08EAC1E090DDD5@EXCHANGEBE2.carle.com> References: <50003EC02E2CEA4583BEB3CD08EAC1E090DDD5@EXCHANGEBE2.carle.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C0121C3B1D8@EXMBMCB15.ucsfmedicalcenter.org> Cryostat decontamination references: Decontamination for HIV Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J Histotechnol, V9, No.1, p.29 (March 1986) Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective against HIV). College of American Pathologists, Commentary on accreditation questionaire: 1) Decontaminate regularly with 70% alcohol. 2) Defrost, remove trimmings and decontaminate with a tuberculocidal disinfectant, preferably weekly for instruments used daily 3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline M29-A. Wayne, PA, NCCLS, 1997 http://www.cap.org/HTML/checklist_html/cklst08p.html NCCLS site: http://www.nccls.org/ Department of Agriculture for Northern Ireland, Safe Working and the Prevention of Infection in Clinical Laboratories, Health Services Advisory Committee. Recommends formalin fuming for 24-48 hours at room temperature. Followed by ammonia for one hour (presumably as a neutralizer). Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill Livingstone, 1994. Recommends defrosting , washing with 70% alcohol, complete cleaning. (no references given). For those who like to read a lot: APIC Guideline for Selection and Use of Disinfectants, Association for Professionals in Infection Control (APIC), American Journal of Infection Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in use and the pros and cons of each. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Friday, January 29, 2010 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decontamination of a Cryostat This issue seems to be rearing its ugly head in our lab once again. What is the correct procedure for decontaminating a cryostat after let's say a specimen from an HIV patient for a frozen was cut on it? We have a couple of different cryostats, one of them defrosts quickly and the other one ices over and defrosts very slowly. Our PA's assist with 98% of the frozens and will use either one of the cryostats. Our pathologists, who perform frozens by themselves on a rare occasion, prefer the cryostat that defrosts very slowly. Of course the cryostat that was contaminated was down awaiting decontamination when a pathologist had to perform a frozen. We are now being told that we need to replace the cryostat that the pathologist doesn't like to use. So have any of you had any issues like this and if you have how did you handle it? Are there any decontamination procedures available for a quick turnaround? Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cory.collins <@t> DHAT.com Fri Jan 29 10:52:26 2010 From: cory.collins <@t> DHAT.com (Cory Collins) Date: Fri Jan 29 10:52:30 2010 Subject: [Histonet] Pathology Laboratory Manager Opening in Houston, Texas Message-ID: SightLine is expanding their service lines and is seeking a pathology laboratory manager who will be responsible for the pathology laboratory services from the design and construction of the new facility, equipment purchasing, hiring staff, policies and procedures, certification and licensure. The ideal candidate must have managed laboratory staff and have been through CLIA certification. They must also have the ability to step-in as a laboratory technician should the situation demanded it. The new facility will be located on South Main near the Reliant Stadium in Houston, Texas Reports to Vice President of Operations. Education: Bachelor Degree in Science with appropriate on the job training. Please indicate your interest by sending emails with resumes attached to hr@sightlinehealth.com From melissa.mazan <@t> tufts.edu Fri Jan 29 11:01:25 2010 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Fri Jan 29 11:01:35 2010 Subject: [Histonet] TUNEL In-Reply-To: <201001291647.o0TGltSp028233@mail-proofpoint-2a> References: <201001291647.o0TGltSp028233@mail-proofpoint-2a> Message-ID: <4B631465.5040600@tufts.edu> Hi all, I am trying to do an apoptosis assay on mouse lung tissue (FFPE), using the Trevigen (formerly Roche) TACs TdT system (fluorescent). I am generating a positive control using nuclease provided by the kit - and get a beautiful, clean, strong signal. Problem is, when I do the same assay using the actual tissues, I get no staining whatsoever. I have included tissues that should have apoptotic cells, including hyperoxia-damaged lung tissue, neonatal tissue, and post-pneumonectomy tissue. When I contact the company, they tell me that if my positive controls are working, then the assay is working, and there is no way to optomize it further. I have already tried cytonin v. Prot K, and Mn2+ v. Co2+, with no improvement. Biologically, the hyperoxia tissues ought to be full of apoptotic cells, and all the others should have at least some. Does anyone have a good protocol or a better kit to suggest? Thanks! Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Ga.Society For Histotechnology Reservation Link > (Shirley A. Powell) > 2. Re: Histonet Digest, Vol 74, Issue 34 (Rhonda Henshall-Powell) > 3. need help with stain (Perry, Margaret) > 4. Re: need help with stain (Rene J Buesa) > 5. Thomas Crowell is out of the office. (thomas.crowell@novartis.com) > 6. RE: [SPAM-HC] - [Histonet] Microtomist's Vade-Mecum on the > Web - Email found in subject (McCormick, James) > 7. RE: Re: Diff-Quik (Tony Henwood) > 8. A blast from the past.... (Jeffrey Silverman) > 9. (no subject) (Green JumpyOne) > 10. honing compound (Jennifer MacDonald) > 11. RE: A blast from the past.... (Mike Pence) > 12. Re : KP Markers (Vanessa Gutierrez) > 13. b-5 (Vickroy, Jim) > 14. Friday Hour of Fun (Breeden, Sara) > 15. RE: b-5 (Weems, Joyce) > 16. job opening (Konni Black) > 17. Decontamination of a Cryostat (Sharon.Davis-Devine) > 18. Re: Decontamination of a Cryostat > (Jan.Minshew@leica-microsystems.com) > 19. RE: Friday Hour of Fun (Mahoney,Janice A) > 20. Kaposi's sarcoma block (Houston, Ronald) > 21. RE: Decontamination of a Cryostat (Morken, Tim) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 28 Jan 2010 13:35:48 -0500 > From: "Shirley A. Powell" > Subject: [Histonet] Ga.Society For Histotechnology Reservation Link > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <9BF995BC0E47744E9673A41486E24EE22429F09B78@MERCERMAIL.MercerU.local> > Content-Type: text/plain; charset="us-ascii" > > Hi All, > > I wanted to pass this link from the hotel on to you guys who will be attending the Georgia Society for Histotechnology meeting March 26-28 this year. This goes straight to the hotel and already has our code entered for you to reserve your rooms at the Evergreen Marriott Conference Resort. For the complete program and registration form go to www.histosearch.com/gsh and go to the symposium page. You will find the hotel link there too. > > See you there. > > Shirley Powell > GSH Secretary > > > > Subject: Ga.Society For Histotechnology Reservation Link > > Simply cut and paste the link below and include with your electronic correspondence to facilitate the reservation process. Your guests will be directed to the property's home page with the code already entered in the appropriate field. All they need to do is enter their arrival date to begin the reservation process. > > > > > > Evergreen Marriott Conference Resort >> > > > > > > [http://www.marriott.com/Images/Brands/MCC/Logos/MCC_logowhitefield_120x60.gif] > > > > > > http://www.marriott.com/hotels/travel/atleg?groupCode=gshgsha&app=resvlink&fromDate=3/26/10&toDate=3/27/10 > > > > > > > > > > > > > > > ------------------------------ > > Message: 2 > Date: Thu, 28 Jan 2010 19:08:33 +0000 (GMT) > From: Rhonda Henshall-Powell > Subject: [Histonet] Re: Histonet Digest, Vol 74, Issue 34 > To: histonet@lists.utsouthwestern.edu > Message-ID: <828544.67370.qm@web23205.mail.ird.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Dear Nick, > > When I was growing and harvesting 3D cell cultures (spheroids grown in matrigel) we would remove the culture medium, draw up the gel in a needle-less syringe and place in to OCT embedding medium (Tissue Tek) before freezing in Liquid Nitrogen. I would then cut 5-10um sections and perform IHC or immunofluorescence. > > Hope this helps - but it looks like you have a lot of good suggestions already. > > Best Regards, > Rhonda > > Rhonda Henshall-Powell, Ph.D. > > >> ------------------------------ >> >> Message: 2 >> Date: Wed, 27 Jan 2010 10:25:42 -0800 (PST) >> From: Nicholas David Evans >> >> Dear all, >> >> I was hoping someone might be able to offer me some advice >> on embedding and sectioning cell cultures. >> >> In short we are growing cells which form 3D dome-like >> structures on tissue culture plastic. Does anyone have any >> experience or advice to offer on embedding the cultures in >> situ before sectioning? I have seen various methods in the >> literature, which often use Epon to embed the material >> followed by sawing away the plastic, but if anyone can offer >> some tips on other possible (easier) ways of doing it, or >> can refer me to some useful literature, I'd be very >> grateful. >> >> I would like to have simple 10um sections at 90 degrees to >> the substrate, which I can use for IHC. >> >> Best wishes >> Nick >> >> >> >> ------------------------------ >> >> Message: 3 >> Date: Wed, 27 Jan 2010 15:19:36 -0500 >> From: Geoff McAuliffe >> >> Hi Nick: >> >> You can use the Epon substitutes such as EmBed 812. Fix, >> osmicate, >> dehydrate as usual, but omit the proplylene oxide as it >> will react with >> the plastic dish. Epon substitutes will mix with ethanol. I >> used 2:1 >> then 1:1 then 1:2 ratios of absolute ethanol to "Epon" with >> catalyst >> added with agitation. Then several changes of pure Epon and >> polymerize. >> Yes, you will have to saw away the plastic, if you try to >> section the >> Epon-plastic combo the two will separate. >> How much easier do you want it to be? >> >> Geoff >> ------------------------------ >> >> Message: 5 >> Date: Wed, 27 Jan 2010 15:39:05 -0500 >> From: "Sherwood, Margaret " >> Subject: RE: [HISTONET] embedding cell cultures >> > > >> Nick, >> >> I am assuming that your 3D cells only grow on >> plastic. They make plastic cover >> slips which, if your cells attach to them and grow, might >> make the embedding >> much easier. You would follow the same method as >> stated, but then you could >> invert the coverslips on a beem capsule and separate the >> coverslip from capsule >> with liquid nitrogen. However, I have never done it >> with plastic coverslips >> (only glass), so not sure if they would easily separate >> from capsule with liquid >> nitrogen. If anyone else has done so, please weigh in. >> >> Peggy >> >> ------------------------------ >> Message: 6 >> Date: Wed, 27 Jan 2010 15:44:37 -0500 >> From: Peggy Bisher >> Subject: Re: [HISTONET] embedding cell cultures >> >> I have done just what you are talking about using Aclar. It >> is a plastic >> embedding film (purchased from EMS). It works great for >> us. >> >> Margaret E. Bisher >> Electron Microscopy & Histology Core Facility Manager >> Department of Molecular Biology >> Princeton University >> Moffett Laboratory, Room 113 >> Princeton, New Jersey >> Office: (609) 258-7026 >> Fax: (609) 258-8468 >> mbisher@princeton.edu >> > > > > > > > ------------------------------ > > Message: 3 > Date: Thu, 28 Jan 2010 15:00:12 -0600 > From: "Perry, Margaret" > Subject: [Histonet] need help with stain > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > A researcher wants to measure the length of the intestinal crypts and asked for a suggestion on what type of stain to use. I am thinking of using a phloxine/tartrazine stain and do a double stain with a GMS to show the basement membrane. What is your opinion on this? > Do you have other suggestions that would work better? > Hope you are warmer than here in SD! > Thanks for the help. > Margaret Perry > South Dakota State University > > > > > > > > > > > > > > > > > > > > > > ------------------------------ > > Message: 4 > Date: Thu, 28 Jan 2010 13:21:17 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] need help with stain > To: "histonet@lists.utsouthwestern.edu" > , MargaretPerry > > Message-ID: <877399.36977.qm@web65714.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Use mucicarmin stain. The crypt mucus will be evident and their sizes. > Ren? J. > > --- On Thu, 1/28/10, Perry, Margaret wrote: > > > From: Perry, Margaret > Subject: [Histonet] need help with stain > To: "histonet@lists.utsouthwestern.edu" > Date: Thursday, January 28, 2010, 4:00 PM > > > A researcher wants to measure the length of the intestinal crypts and asked for a suggestion on what type of stain to use. I am thinking of using a phloxine/tartrazine stain and do a double stain with a GMS to show the basement membrane. What is your opinion on this? > Do you have other suggestions that would work better? > Hope you are warmer than here in SD! > Thanks for the help. > Margaret Perry > South Dakota State University > > > > > > > > > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 5 > Date: Thu, 28 Jan 2010 16:24:40 -0500 > From: thomas.crowell@novartis.com > Subject: [Histonet] Thomas Crowell is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 01/28/2010 and will not return until > 02/02/2010. > > Please contact Kelly Miner at 617-871-5122 if you have any questions > regarding clinical trial samples. > > ------------------------------ > > Message: 6 > Date: Thu, 28 Jan 2010 15:29:09 -0600 > From: "McCormick, James" > Subject: RE: [SPAM-HC] - [Histonet] Microtomist's Vade-Mecum on the > Web - Email found in subject > To: Robert Richmond , > "Histonet@lists.utsouthwestern.edu" > > Message-ID: > <0CA58D7DD405854899D129A03276B1334439313EA3@EXCHCCRMB.schosp.org> > Content-Type: text/plain; charset="us-ascii" > > Samuri......... > The website www.scienceheritagelimited.com has available 8 books that are reprints of historically important "history of microscopy and histotechnology books" included is the 1885 edition of Arthur Bolles Lee's "The Microtomist's Vade-Mecum". It's amazing how little (relatively) things have changed. The only thing missing in the 1885 edition is the use of formalin as a tissue fixative. This did not come about until 1892 and undoubtedly appears in his later editions. > Good reading, > > jim > J.B.McCormick, M.D. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Thursday, January 28, 2010 7:42 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [SPAM-HC] - [Histonet] Microtomist's Vade-Mecum on the Web - Email found in subject > > Arthur Bolles Lee's The Microtomist's Vade-Mecum, 7th edition (1913) > is available online on Google Books, along with some other editions: > > http://books.google.com/books?id=4m5MAAAAMAAJ&printsec=frontcover&dq=arthur+bolles+lee&ei=BpJhS96xKJTIzASV9vnNBQ&cd=1#v=onepage&q=&f=false > > This marvelous old histology recipe book was a great rarity before the > Web, though it's actually now available from Amazon. It took me > several years to find a copy in 1976 - found one in a consignment of > old books discarded from the library of a long-defunct college. > > A note about the title: Vade-Mecum (pronounced something like > voddy-make-um) - literally "come with me" (think of "Quo Vadis) is an > old word for a practical handbook of something. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > > Thank you for your cooperation. > > > > ------------------------------ > > Message: 7 > Date: Fri, 29 Jan 2010 09:21:55 +1100 > From: "Tony Henwood" > Subject: RE: [Histonet] Re: Diff-Quik > To: , "Robert Richmond" > Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Please don't take it personally. > > If we are trying our best to meet our own high expectations then we do > not have to worry. > I believe that most of us do what Robert expects. This is supported by > my experiences at the recent NSH meeting in Birmingham Alabama. I was > privileged to meet some of the most dedicated professionals around and > believe that our profession is heading in the right direction. > > Robert's comments should be heeded and pondered. I definitely did. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Kim.Donadio@bhcpns.org > Sent: Thursday, 28 January 2010 2:53 AM > To: Robert Richmond > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re: Diff-Quik > > > I'm going to have to agree with Cheryl on the comment. This may be your > experience but I can tell you my techs always look at their stains > before > they send it on to the Pathologist. It is a requirement that they > understand what they are looking at in order to know if it worked. Each > of > them are also trained to know all tissues microscopically and all stain > components microscopically. That is after all the purpose of being a > Histologist. > > I am going out on a limb here and I normally don't, but you are digging > yourself in to a rather rude hole to insult so many professional > Histologist. > > Just saying............. > > > Kim Donadio > Pathology Supervisor > Baptist Hospital > 1000 W Moreno St. > Pensacola FL 32501 > Phone (850) 469-7718 > Fax (850) 434-4996 > > > > Robert Richmond > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/26/2010 07:38 PM > > To > "histonet@lists.utsouthwestern.edu" > cc > > Subject > [Histonet] Re: Diff-Quik > > > > > > > I sort of apologize for this ill-natured comment, which long-term > readers of Histonet know I've made before. > > I do locum tenens work, mostly in rather small pathology services - I've > worked in perhaps 60 of them in my life. Only rarely do I observe that a > histotech ever looks at a slide. I've just acquired a new client with > particularly difficult slides. The tech doesn't even have a microscope. > > The more quality assurance paperwork I have to do, the worse the slides. > > The lack of feedback from pathologist to technologist is a really > widespread and serious problem. Most pathologists are completely > unwilling to take the time to do it, and the usage has never established > itself. It would be much easier if we had double headed microscopes, > which seem to be prohibited in small pathology services. > > Did Edwards Deming live in vain? > > Bob Richmond > Samurai Pathologist > Knoxville TN > ************************************* > On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller > wrote: > >> Every slide I stain, special stains, IHC or otherwise I check under >> the >> > scope...I have taught all my techs to do the same, other than batches of > > H&E and then we check the 1st slide in each rack. I know this to be a > common procedure with many histology professionals. The attitude can be > left in your lab please. Thank you > >> Cheryl Miller HT ASCP CM >> Histology Supervisor >> Physicians Laboratory Services >> Omaha, NE. 402 731 4148 >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert > Richmond > >> Sent: Monday, January 25, 2010 7:50 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Re: Diff-Quik >> >> Thanks, John Kiernan, for your explanation of Romanovsky stains. >> >> "Diff-Quik" (please note the spelling) is the trademarked name of a >> staining sequence consisting of a fixative, eosin (Diff-Quik I), and >> an azure (Diff-Quik II), done in that order in three separate >> containers. I'm not sure who the trademark presently belongs to - it >> seems to change with the phases of the Moon. >> >> There are a number of generic equivalents, which in my personal >> experience all work as well as trademark Diff-Quik. For most ordinary >> pathology services, it isn't worthwhile to try to brew your own. >> >> I don't think I've seen bone marrow stained with such a sequence. >> Proper staining of bone marrows requires that the histotechnologist >> examine the slides under a microscope, a practice too many find >> abhorrent. >> >> Bob Richmond >> Samurai Pathologist >> Knoxville TN >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----------------------------------------- > All electronic data transmissions originating from or sent to Baptist > Health Care Corporation (BHC) are subject to monitoring. This message > along with any attached data, are the confidential and proprietary > communications of BHC and are intended to be received only by the > individual or individuals to whom the message has been addressed. If the > reader of this message is not the intended recipient, please take notice > that any use, copying, printing, forwarding or distribution of this > message, in any form, is strictly prohibited and may violate State or > Federal Law. If you have received this transmission in error, please > delete or destroy all copies of this message. For questions, contact > the BHC Privacy Officer at (850) 434-4472. Rev.10/07. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 8 > Date: Thu, 28 Jan 2010 14:53:25 -0800 (PST) > From: Jeffrey Silverman > Subject: [Histonet] A blast from the past.... > To: histonet@lists.utsouthwestern.edu > Message-ID: <276280.46298.qm@web111112.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Dr Richmond, > > Thanks so much for that link to Vade Mecum. I've never before seen the book, but it has brought me back to my childhood, when I found a similar histotechnique book, Michael Guyer's Animal Micrology, in an antique shop my mom was browsing, some 44 years ago. This book was referenced all over that book, and in Gray's Microtomist's Formulary and Guide which I obtained later as my interest in histotechnique grew during high school, an interest kindled by that book and resulting in a rewarding and fascinating career. I feel like a kid again. Very cool. > > It's amazing how much mercury they slung around back then. Hell, I made my own Zenker's fluid in high school from those books, bet the waste went down the drain too. > > Jeff Silverman > > ------------------------------ > > Message: 9 > Date: Thu, 28 Jan 2010 16:38:59 -0800 > From: Green JumpyOne > Subject: [Histonet] (no subject) > To: , , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > http://www.nesle2005.republika.pl/T6vJjAE12x.html > _________________________________________________________________ > Hotmail: Trusted email with powerful SPAM protection. > http://clk.atdmt.com/GBL/go/196390707/direct/01/ > > > ------------------------------ > > Message: 10 > Date: Thu, 28 Jan 2010 22:03:34 -0800 > From: Jennifer MacDonald > Subject: [Histonet] honing compound > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > A colleague from a neighboring university is trying to find both fine and > coarse honing compound for his knife sharpener. Does anyone have a good > source? > > Thank you, > Jennifer MacDonald > > ------------------------------ > > Message: 11 > Date: Fri, 29 Jan 2010 08:13:30 -0600 > From: "Mike Pence" > Subject: RE: [Histonet] A blast from the past.... > To: "Jeffrey Silverman" , > > Message-ID: > <661949901A768E4F9CC16D8AF8F2838C017A3D28@is-e2k3.grhs.net> > Content-Type: text/plain; charset="iso-8859-1" > > How true!.... Can you believe the things we use to put down the drain or in the trash? "Out of sight, out of mind". Today a high chemistry lab would be shut down and the teacher sent to jail for some of the things they taught us back then. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman > Sent: Thursday, January 28, 2010 4:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] A blast from the past.... > > > Dr Richmond, > > Thanks so much for that link to Vade Mecum. I've never before seen the book, but it has brought me back to my childhood, when I found a similar histotechnique book, Michael Guyer's Animal Micrology, in an antique shop my mom was browsing, some 44 years ago. This book was referenced all over that book, and in Gray's Microtomist's Formulary and Guide which I obtained later as my interest in histotechnique grew during high school, an interest kindled by that book and resulting in a rewarding and fascinating career. I feel like a kid again. Very cool. > > It's amazing how much mercury they slung around back then. Hell, I made my own Zenker's fluid in high school from those books, bet the waste went down the drain too. > > Jeff Silverman > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 12 > Date: Fri, 29 Jan 2010 06:41:34 -0800 (PST) > From: Vanessa Gutierrez > Subject: [Histonet] Re : KP Markers > To: Histonet@lists.utsouthwestern.edu > Message-ID: <738912.31896.qm@web30206.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > KP Marker pens are sold by Mercedes Medical. I use them and I really like them. The box I ordered is still on back order so you want to order as soon as possible. > > > > > ------------------------------ > > Message: 13 > Date: Fri, 29 Jan 2010 09:21:49 -0600 > From: "Vickroy, Jim" > Subject: [Histonet] b-5 > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > <24A4826E8EF0964D86BC5317306F58A54256B2E3FA@mmc-mail.ad.mhsil.com> > Content-Type: text/plain; charset="us-ascii" > > > Someone remind me. (senior moment). Years ago we stopped using B-5 fixative in our lab. If I remember right we were given a date by CAP to either stop using a mercury product or present a plan on when we would stop using it. Am I correct and if not are some people still using B-5? > > > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > > > ------------------------------ > > Message: 14 > Date: Fri, 29 Jan 2010 08:37:24 -0700 > From: "Breeden, Sara" > Subject: [Histonet] Friday Hour of Fun > To: "histonet" > Message-ID: > <4D14F0FC9316DD41972D5F03C070908B02E46E12@nmdamailsvr.nmda.ad.nmsu.edu> > > Content-Type: text/plain; charset="us-ascii" > > I have a Hypothetical Situation for which I would like your input. If > you - as a histologist - were to be involved in the interview of a > potential staff pathologist, what questions would you ask this > candidate? Think of the practical and logical issues, but also the > unique and pertinent secondary Things You'd Like to Know. I would like > input from techs AND pathologists. If you were a pathologist in an > interview situation, what questions would you think appropriate? If > you're a tech, what would you like to know about this candidate before > he begins to orbit your universe? > > > > Please write me off Histonet - not for any other reason than it's likely > that everyone will not be interested in this subject. Thank you again > and I can't wait to see what answers I will get! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > > > ------------------------------ > > Message: 15 > Date: Fri, 29 Jan 2010 10:42:08 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] b-5 > To: "Vickroy, Jim" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Shhhhhhhhhhhhhhhhh, if I understand correctly, they are not legal. It > was several years ago that we were mandated to stop. j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, > Jim > Sent: Friday, January 29, 2010 10:22 > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] b-5 > > > Someone remind me. (senior moment). Years ago we stopped using B-5 > fixative in our lab. If I remember right we were given a date by CAP to > either stop using a mercury product or present a plan on when we would > stop using it. Am I correct and if not are some people still using > B-5? > > > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor Memorial Medical > Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential > information intended for a specific individual and purpose, and is > protected by law. If you are not the intended recipient, you should > delete this message. Any disclosure, copying, or distribution of this > message, or the taking of any action based on it, is strictly > prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > > > ------------------------------ > > Message: 16 > Date: Fri, 29 Jan 2010 08:04:28 -0800 > From: "Konni Black" > Subject: [Histonet] job opening > To: "histonet" > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > We are looking for a fulltime histologist for a new lab in Olympia, WA. Planned opening in March with great new equipment and lab space. The ideal candidate will have 3 to 5 years experience and must be able to work alone. Flexible work schedule. . > > Contact Konni Black > kblack@digestivehlth.com > 253-503-2560 > > > > ------------------------------ > > Message: 17 > Date: Fri, 29 Jan 2010 10:16:16 -0600 > From: "Sharon.Davis-Devine" > Subject: [Histonet] Decontamination of a Cryostat > To: > Message-ID: > <50003EC02E2CEA4583BEB3CD08EAC1E090DDD5@EXCHANGEBE2.carle.com> > Content-Type: text/plain; charset="us-ascii" > > This issue seems to be rearing its ugly head in our lab once again. > What is the correct procedure for decontaminating a cryostat after let's > say a specimen from an HIV patient for a frozen was cut on it? We have > a couple of different cryostats, one of them defrosts quickly and the > other one ices over and defrosts very slowly. Our PA's assist with 98% > of the frozens and will use either one of the cryostats. Our > pathologists, who perform frozens by themselves on a rare occasion, > prefer the cryostat that defrosts very slowly. Of course the cryostat > that was contaminated was down awaiting decontamination when a > pathologist had to perform a frozen. We are now being told that we need > to replace the cryostat that the pathologist doesn't like to use. So > have any of you had any issues like this and if you have how did you > handle it? Are there any decontamination procedures available for a > quick turnaround? Any help or suggestions will be greatly appreciated. > Thanks. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology-Histology Supervisor > > Carle Foundation Hospital > > Laboratory and Pathology Services > > 611 West Park Street > > Urbana, Illinois 61801 > > 217-383-3572 > > sharon.davis-devine@carle.com > > > > > > ------------------------------ > > Message: 18 > Date: Fri, 29 Jan 2010 10:34:29 -0600 > From: Jan.Minshew@leica-microsystems.com > Subject: Re: [Histonet] Decontamination of a Cryostat > To: "Sharon.Davis-Devine" > Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=ISO-8859-1 > > > Hi Sharon, > > I copied and pasted a chemical disinfection procedure for cryostats that I > created for Leica's sales and service employees and customers. It > references Leica cryostats but it can be used for any cryostat--the basic > principles for chemical disinfection are the same. Since we can't put > attachments on the histonet, I am hoping that you will be able to read the > copy without problem. If any of you have trouble, please let me know and I > can send it to you off line as an attachment. > > > Chemical Disinfection of a Cryostat > > According to CAP regulations, a cryostat should be defrosted and > decontaminated with a tuberculocidal disinfectant at a time interval > appropriate for the institution (once a week for instruments used daily). > The regulations also state that the cryostat must be clearly marked as > contaminated if a frozen section is performed on tissue from a patient > known or suspected to be positive for HIV, hepatitis B or C, SARS-related > coronavirus, prion disease such as Creutzfeldt-Jakob disease, or > mycobacterial or systemic fungal disease. It must then be decontaminated > before further use. > > ? Wear Personal Protective Equipment (PPE): > Personal Protective Equipment, such as gowns, puncture and penetration > resistant gloves and eye protection must be worn when performing cryostat > disinfection procedures. > > ? Cryostat Preparation > Remove used blades/knives from their holder. Although not a requirement, > steel mesh gloves should be worn when changing knife blades. Dispose of > blades according to the regulations of the institution or disinfect > knives before reusing by soaking in disinfecting solution. Remove all > debris and utensils (pencils, forceps, brushes, gauze, etc) from the > chamber. Debris must be removed because organic material (blood and > proteins) may contain high concentrations of microorganisms and could > possibly inactivate the chemical disinfectant or prevent access to > contaminated surfaces. It should be treated as biohazardous waste and > disposed of according to the policies and procedures of the institution. > > 70% ethyl or reagent alcohol can be used to clean the cryostat and > provide some disinfection capabilities. The germicidal activity of ethyl > alcohol is most effective in the 70% range because it can penetrate > tubercle bacteria and it has an advantage over isopropyl alcohol of being > able to kill hydrophilic viruses. > > ? Chemical Disinfection > In order to disinfect a cryostat using a chemical disinfectant, the > instrument MUST be at room temperature before the process is started. > Turn off and unplug the instrument before beginning the disinfection > process. (Once the cryostat has reached room temperature, do not turn > the handwheel until it has been returned to cold temperature.) > > Chemical Disinfection of a Cryostat continued: > > Do not create aerosols by spraying disinfectant (or anything else) in an > open cryostat chamber. Pour disinfectants onto surfaces or absorbent > disposable towels and allow them to remain in contact with contaminated > surfaces for the length of time specified in the instructions of the > individual agents. Use a tuberculocidal disinfectant that is > non-corrosive. The EPA maintains a list of Antimicrobial Chemical/ > Registration Number Indexes on their website, > http://www.epa.gov/oppad001/chemregindex.htm), and it is updated > regularly. From this link you can find agents effective against > bloodborne pathogens such as Mycobacterium tuberculosis, human HIV-1 > virus, and Hepatitis B or Hepatitis C virus. It is critical to remember > that NONE of these solutions have been tested at low temperatures and can > only be used at room temperature. > > Any disposable material used in the disinfection process must be disposed > of in accordance with the policies and procedures of the institution. > > ? Following Disinfection > After the disinfection procedure is complete, the cryostat and all of the > accessories must be thoroughly dried and lubricated before being put back > into service at cold temperatures. Absolute ethyl alcohol can be used to > remove excess moisture from surfaces. Accessories with multiple parts, > such as the disposable blade holder, knife holder and their respective > bases, must be taken apart and dried thoroughly. > > Plug the instrument back in and turn it on. Use only the lubricants that > are recommended by the manufacturer and only in the recommended amounts. > Lightly lubricate any moving parts. To lubricate the specimen arm, > extend it all the way forward. Apply ONE DROP of lubricant to the barrel > and spread it around with your gloved finger. Move the arm back to the > home position. > > The liquid waste container should be emptied in accordance with the > policies and procedures of the institution. Before replacing in the > instrument, add a small amount of liquid bleach to the empty container. > > For optimum sectioning, allow the instrument to cool long enough to allow > the metal microtome parts to reach the cold temperature setting. The > CM1850 requires no less than 3 hours and the CM1950 needs 5 hours to cool > down from ambient temperature (20?C) to -25?C and 8 hours to cool from > 20?C to -35?C. > > > > > > > > Kind regards, > > Jan Minshew > Marketing Manager > Leica Microsystems > Biosystems Division > 2345 Waukegan Road > Bannockburn, IL 60015 > > Office: 847.405.7051 > Cell: 847.970.8468 > Fax: 847.405.6560 > > www.leica-microsystems.com > > > Click Here for this month's special offers! > http://www.leica-microsystems.com/bsdspecial > > > > > "Sharon.Davis-Dev > ine" > ine@carle.com> > Sent by: cc > histonet-bounces@ > lists.utsouthwest Subject > ern.edu [Histonet] Decontamination of a > Cryostat > > 01/29/2010 10:16 > AM > > > > > > > > This issue seems to be rearing its ugly head in our lab once again. > What is the correct procedure for decontaminating a cryostat after let's > say a specimen from an HIV patient for a frozen was cut on it? We have > a couple of different cryostats, one of them defrosts quickly and the > other one ices over and defrosts very slowly. Our PA's assist with 98% > of the frozens and will use either one of the cryostats. Our > pathologists, who perform frozens by themselves on a rare occasion, > prefer the cryostat that defrosts very slowly. Of course the cryostat > that was contaminated was down awaiting decontamination when a > pathologist had to perform a frozen. We are now being told that we need > to replace the cryostat that the pathologist doesn't like to use. So > have any of you had any issues like this and if you have how did you > handle it? Are there any decontamination procedures available for a > quick turnaround? Any help or suggestions will be greatly appreciated. > Thanks. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology-Histology Supervisor > > Carle Foundation Hospital > > Laboratory and Pathology Services > > 611 West Park Street > > Urbana, Illinois 61801 > > 217-383-3572 > > sharon.davis-devine@carle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > > > > ------------------------------ > > Message: 19 > Date: Fri, 29 Jan 2010 10:44:04 -0600 > From: "Mahoney,Janice A" > Subject: [Histonet] RE: Friday Hour of Fun > To: "'Breeden, Sara'" , histonet > > Message-ID: > <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A562C@EXCHMBC2.ad.ah.local> > Content-Type: text/plain; charset="iso-8859-1" > > I'd ask how he sees his relationship with the histotechnologists, histo supervisor, etc. > I'd ask him what his expectation is regarding TAT of slides/special stains/IHC/rectus. > If there is more than one Pathologist, I'd ask how they would collaborate to decide on standard work. For example, will you cut one two or three slides on GI bxs. (in other words will he accept that you will not do specific things for individual Pathologists, that they will decide on standard work). > these are a few thing off the top of my head. > Good luck, it will be interesting to see what others come up with. > Jan Mahoney > Omaha, N?E > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Friday, January 29, 2010 9:37 AM > To: histonet > Subject: [Histonet] Friday Hour of Fun > > I have a Hypothetical Situation for which I would like your input. If > you - as a histologist - were to be involved in the interview of a > potential staff pathologist, what questions would you ask this > candidate? Think of the practical and logical issues, but also the > unique and pertinent secondary Things You'd Like to Know. I would like > input from techs AND pathologists. If you were a pathologist in an > interview situation, what questions would you think appropriate? If > you're a tech, what would you like to know about this candidate before > he begins to orbit your universe? > > > > Please write me off Histonet - not for any other reason than it's likely > that everyone will not be interested in this subject. Thank you again > and I can't wait to see what answers I will get! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. > > > > > ------------------------------ > > Message: 20 > Date: Fri, 29 Jan 2010 11:45:00 -0500 > From: "Houston, Ronald" > Subject: [Histonet] Kaposi's sarcoma block > To: , > Message-ID: > <979FF5962E234F45B06CF0DB7C1AABB21B646032@chi2k3ms01.columbuschildrens.net> > > Content-Type: text/plain; charset="us-ascii" > > Can anyone spare a block of Kaposi's sarcoma? > > Thanks > > > > Ronnie Houston, MS HT(ASCP)QIHC > > Anatomic Pathology Manager > > ChildLab, a Division of Nationwide Children's Hospital > > www.childlab.com > > 700 Children's Drive > > Columbus, OH 43205 > > (P) 614-722-5450 > > (F) 614-722-2899 > > ronald.houston@nationwidechildrens.org > > www.NationwideChildrens.org > > > > "One person with passion is better than forty people merely interested." > ~ E.M. Forster > > > > > > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > > ------------------------------ > > Message: 21 > Date: Fri, 29 Jan 2010 08:45:45 -0800 > From: "Morken, Tim" > Subject: [Histonet] RE: Decontamination of a Cryostat > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1AAF670737F193429070841C6B2ADD4C0121C3B1D8@EXMBMCB15.ucsfmedicalcenter.org> > > Content-Type: text/plain; charset=us-ascii > > Cryostat decontamination references: > > > Decontamination for HIV > Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J Histotechnol, V9, No.1, p.29 (March 1986) > Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective against HIV). > > > College of American Pathologists, Commentary on accreditation questionaire: > 1) Decontaminate regularly with 70% alcohol. > 2) Defrost, remove trimmings and decontaminate with a tuberculocidal disinfectant, preferably weekly for instruments used daily > 3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline M29-A. Wayne, PA, NCCLS, 1997 > http://www.cap.org/HTML/checklist_html/cklst08p.html > > NCCLS site: http://www.nccls.org/ > > > > Department of Agriculture for Northern Ireland, Safe Working and the Prevention of Infection in Clinical Laboratories, Health Services Advisory Committee. > Recommends formalin fuming for 24-48 hours at room temperature. Followed by ammonia for one hour (presumably as a neutralizer). > > Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill Livingstone, 1994. > Recommends defrosting , washing with 70% alcohol, complete cleaning. (no references given). > > For those who like to read a lot: > APIC Guideline for Selection and Use of Disinfectants, Association for Professionals in Infection Control (APIC), American Journal of Infection Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in use and the pros and cons of each. > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > San Francisco, CA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine > Sent: Friday, January 29, 2010 8:16 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Decontamination of a Cryostat > > This issue seems to be rearing its ugly head in our lab once again. > What is the correct procedure for decontaminating a cryostat after let's > say a specimen from an HIV patient for a frozen was cut on it? We have > a couple of different cryostats, one of them defrosts quickly and the > other one ices over and defrosts very slowly. Our PA's assist with 98% > of the frozens and will use either one of the cryostats. Our > pathologists, who perform frozens by themselves on a rare occasion, > prefer the cryostat that defrosts very slowly. Of course the cryostat > that was contaminated was down awaiting decontamination when a > pathologist had to perform a frozen. We are now being told that we need > to replace the cryostat that the pathologist doesn't like to use. So > have any of you had any issues like this and if you have how did you > handle it? Are there any decontamination procedures available for a > quick turnaround? Any help or suggestions will be greatly appreciated. > Thanks. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology-Histology Supervisor > > Carle Foundation Hospital > > Laboratory and Pathology Services > > 611 West Park Street > > Urbana, Illinois 61801 > > 217-383-3572 > > sharon.davis-devine@carle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 74, Issue 36 > **************************************** > -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cummings School of Veterinary Medicine 200 Westborough Road North Grafton,MA 01536 tel: 508-839-5395 fax: 508-839-7903 email: melissa.mazan@tufts.edu From cbrya <@t> lexclin.com Fri Jan 29 11:01:34 2010 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Fri Jan 29 11:01:39 2010 Subject: [Histonet] RE: Decontamination of a Cryostat In-Reply-To: <1AAF670737F193429070841C6B2ADD4C0121C3B1D8@EXMBMCB15.ucsfmedicalcenter.org> References: <50003EC02E2CEA4583BEB3CD08EAC1E090DDD5@EXCHANGEBE2.carle.com> <1AAF670737F193429070841C6B2ADD4C0121C3B1D8@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF013BBC50F7@EXCHANGESB> What about instruments that have a built-in defrost and disinfect process with formalin? Is that sufficient to meet CAP's requirements? We do it weekly at our lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, January 29, 2010 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Decontamination of a Cryostat Cryostat decontamination references: Decontamination for HIV Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J Histotechnol, V9, No.1, p.29 (March 1986) Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective against HIV). College of American Pathologists, Commentary on accreditation questionaire: 1) Decontaminate regularly with 70% alcohol. 2) Defrost, remove trimmings and decontaminate with a tuberculocidal disinfectant, preferably weekly for instruments used daily 3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline M29-A. Wayne, PA, NCCLS, 1997 http://www.cap.org/HTML/checklist_html/cklst08p.html NCCLS site: http://www.nccls.org/ Department of Agriculture for Northern Ireland, Safe Working and the Prevention of Infection in Clinical Laboratories, Health Services Advisory Committee. Recommends formalin fuming for 24-48 hours at room temperature. Followed by ammonia for one hour (presumably as a neutralizer). Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill Livingstone, 1994. Recommends defrosting , washing with 70% alcohol, complete cleaning. (no references given). For those who like to read a lot: APIC Guideline for Selection and Use of Disinfectants, Association for Professionals in Infection Control (APIC), American Journal of Infection Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in use and the pros and cons of each. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Friday, January 29, 2010 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decontamination of a Cryostat This issue seems to be rearing its ugly head in our lab once again. What is the correct procedure for decontaminating a cryostat after let's say a specimen from an HIV patient for a frozen was cut on it? We have a couple of different cryostats, one of them defrosts quickly and the other one ices over and defrosts very slowly. Our PA's assist with 98% of the frozens and will use either one of the cryostats. Our pathologists, who perform frozens by themselves on a rare occasion, prefer the cryostat that defrosts very slowly. Of course the cryostat that was contaminated was down awaiting decontamination when a pathologist had to perform a frozen. We are now being told that we need to replace the cryostat that the pathologist doesn't like to use. So have any of you had any issues like this and if you have how did you handle it? Are there any decontamination procedures available for a quick turnaround? Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From kryan <@t> nfderm.com Fri Jan 29 11:29:20 2010 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Fri Jan 29 11:29:25 2010 Subject: [Histonet] looking for Marvin Hanna Message-ID: Does anyone have contact information for him? Thanks, Kaye Ryan North Florida Dermatology Associates Histology Lab Supervisor (904) 398-0547 Ext. 2004 From lhotaks <@t> mcmaster.ca Fri Jan 29 12:37:20 2010 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Fri Jan 29 12:38:09 2010 Subject: [Histonet] TUNEL Message-ID: Hi Melissa, I use the Trevigen system with great success on mouse tissue using biotinylated dNTPs, Streptavidin HRP and NovaREd. It should work just as well with fluorescence. Mouse thymus is a great positive control. My protocol is as follows: -Xylenes, ethanols, water. -Proteinase K 30 minutes at 37C -Endog. peroxidase quenching: 45 ml methanol+5ml 30% H2O2, 5 min. -1x labeling buffer, 5 min -Reaction mixture: 1x labeling b. 50ul + TdTdNTP mix 1ul+ 50xCo2+ 1ul + TdT enzyme 1ul. 1hr at 37C. -1x Stop buffer, 5 min RT Then I use the Streptavidin HRP and Nova Red that I use for IHC, exactly the same way. I found that the Endog. peroxidase quenching step was ESSENTIAL for this reaction to work, i.e. probably doing more than just quenching, without that there was NO reaction at all. Hope this helps somewhat, don't hesitate to contact me if you have more questions. The beauty of the Trevigen system is that you can buy individual reagents, no need to be paying big bucks for a full kit. (No commercial interest in Trevigen :), just a happy customer). Sarka Lhotak McMaster University, Hamilton From NKonop <@t> chw.org Fri Jan 29 13:01:40 2010 From: NKonop <@t> chw.org (Konop, Nicole) Date: Fri Jan 29 13:01:46 2010 Subject: [Histonet] Processing brain biopsies on Leica Peloris Message-ID: I'm looking for feedback on processing and processing programs for brain biopsy cases using the Peloris. My main focus is pediatric brain biopsy cases. We are in the stages of justifying a new processor and I need feedback regarding brain biopsy turn around time on brain biopsies. Any feedback regarding this issue is appreciated. Thanks! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)266-2524 Histology Department From ean.moody <@t> mercedesmedical.com Fri Jan 29 13:47:31 2010 From: ean.moody <@t> mercedesmedical.com (Ean Moody) Date: Fri Jan 29 13:47:39 2010 Subject: [Histonet] Re: Histonet Digest, Vol 74, Issue 36 In-Reply-To: Message-ID: Mercedes Medical has also launched a couple new domains just for KP Pens, since people have such a hard time finding them. www.kpmarker.com www.kppen.com If anyone else asks, you can send them there! -- Ean On 1/29/10 11:53 AM, "histonet-request@lists.utsouthwestern.edu" wrote: > Message: 12 > Date: Fri, 29 Jan 2010 06:41:34 -0800 (PST) > From: Vanessa Gutierrez > Subject: [Histonet] Re : KP Markers > To: Histonet@lists.utsouthwestern.edu > Message-ID: <738912.31896.qm@web30206.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > KP?Marker pens are sold?by Mercedes Medical. I use them and I really like > them.?The?box I ordered is still on back order so you want to order as soon as > possible. From sbreeden <@t> nmda.nmsu.edu Fri Jan 29 13:53:39 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jan 29 13:54:00 2010 Subject: [Histonet] Hypothetical Pathologist Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46E29@nmdamailsvr.nmda.ad.nmsu.edu> Thank you to all who have responded to my Hypothetical Pathologist Interview scenario! I have accumulated a nice bunch of Hypothetical Questions and I have until Tuesday, Feb. 2 at 3:00 pm NM time to finish my project - I would like very much to hear from you until then. After I work over the list and fine-tune it for the Hypothetical Circumstances, I would be happy to send you a copy of the list of questions if you are interested. If so, please contact me off-list. I AM looking for pathologist's input as well. Answers will not be cited to any individual. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From rjbuesa <@t> yahoo.com Fri Jan 29 14:51:32 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 29 14:51:47 2010 Subject: [Histonet] Friday Hour of Fun In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46E12@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <370836.76696.qm@web65703.mail.ac4.yahoo.com> Sara: Even when you suggested to answer you directly I always prefer to answer to the list so anybody can agree/disagree/expand. ? I don't see the point in asking a pathologist specific?"things histology" because they usually do not train on this matters, but I would like to know: ? 1- what s/he does not know and I would find out by asking what s/he knows, especially with regards to the time required to complete histology tasks, 2- I would like to know if s/he is willing to be open with me and tell me when I do something wrong or with below average quality (for his/her standards), and 3- let him/her know that the least s/he can do is letting me know when I do something that s/he considers of good quality. ? That would establish a professional relationship that is the essence of the pathologist/histotechnologist relationship. Anything else are personality issues that would never be solved by Q/A session with the PT. Ren? J.? ? ? --- On Fri, 1/29/10, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Friday Hour of Fun To: "histonet" Date: Friday, January 29, 2010, 10:37 AM I have a Hypothetical Situation for which I would like your input.? If you - as a histologist - were to be involved in the interview of a potential staff pathologist, what questions would you ask this candidate?? Think of the practical and logical issues, but also the unique and pertinent secondary Things You'd Like to Know.? I would like input from techs AND pathologists.? If you were a pathologist in an interview situation, what questions would you think appropriate?? If you're a tech, what would you like to know about this candidate before he begins to orbit your universe? Please write me off Histonet - not for any other reason than it's likely that everyone will not be interested in this subject.? Thank you again and I can't wait to see what answers I will get! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Fri Jan 29 14:59:51 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Jan 29 15:00:02 2010 Subject: [Histonet] Klotz fixative In-Reply-To: <20100129180050.BBAFE7711F5@barracuda.sdstate.edu> References: <20100129180050.BBAFE7711F5@barracuda.sdstate.edu> Message-ID: Do you know of anyone that sells Klotz fixative? We use it in a pre vet class but would like to eliminate the choloralhydrate since it is such a hassel due to it's being a controlled substance. Is there a substitute fixative that would retain the properties of Klotz? From kc <@t> ka-recruiting.com Fri Jan 29 17:50:49 2010 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Fri Jan 29 17:50:56 2010 Subject: [Histonet] Histology Jobs!!!! Message-ID: <1523839748.1264809049088.JavaMail.cfservice@webserver54> Hi Histoneters, Are you interested in hearing about new job opportunities? I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. Below is a list of some of the other Histology opportunities we are currently working on. Histotechs/Cytotechs: ****Northern, VA - Histotechnologist 2nd shift **** New Opening!!! ****Las Vegas, NV - Histology Supervisor **** New Opening!!! Connecticut- Histology Operations Manager Southern CA - Histology Supervisor New York City - Surgical Pathology and Histology Supervisor New York City - Histotech 3rd shift Las Vegas, NV - Histotech 3rd shift Central Georgia - Histotech 1st shift Oklahoma - Histotech 1st shift (with opportunity to be promoted to supervisor) Long Island, NY - Cytotech New York City - Cytotech Pennsylvania - Cytology Supervisor Connecticut ? Cytotech If you're interested in learning more about any of these opportunities then please email me a resume and let me know how best to get in touch with you. If none of these are a fit please let me know what you'd be interested in and where you're looking so I can tailor a search for you. With the New Year upon us many of our clients have fresh hiring budgets and will be looking to add people over the next several months. We work on positions at all levels and cover the entire US. To view some additional opportunities please visit our website at www.ka-recruiting.com . Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From jkiernan <@t> uwo.ca Sat Jan 30 00:23:37 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Jan 30 00:23:42 2010 Subject: [Histonet] Friday Hour of Fun. Answer to ,,, In-Reply-To: <370836.76696.qm@web65703.mail.ac4.yahoo.com> References: <4D14F0FC9316DD41972D5F03C070908B02E46E12@nmdamailsvr.nmda.ad.nmsu.edu> <370836.76696.qm@web65703.mail.ac4.yahoo.com> Message-ID: YES cubed and in spades! The whole idea of Histonet is that everyone can read the questions, replies, opinions and discussion. Well said, Rene Buesa! John Kiernan Anatomy, UWO London, Canada = = = Rene J Buesa rjbuesa@yahoo.com wrote: > Even when you suggested to answer you directly I always prefer > to answer to the list so anybody can agree/disagree/expand. From kdwyer3322 <@t> aol.com Sat Jan 30 12:22:59 2010 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sat Jan 30 12:23:20 2010 Subject: [Histonet] TEXAS SOCIETY FOR HISTOTECHNOLOGY 2010 MEETING Message-ID: <8CC6FF062AFC0C9-26F0-D6A2@webmail-d008.sysops.aol.com> All Histonetters: The program is complete for the Texas Society for Histotechnology 2010 State Meeting in Houston Texas. If you would like an electronic copy of the program please contact me via this e-mail. All meeting and workshop information should be posted on the website shortly. Regards, TSH Convention Committee Texas Society for Histotechnology, Inc. 33rdAnnual Symposium/Convention ?Histology: A Kaleidoscope of Change Omni Houston Hotel Westside 13210 Katy Freeway Houston, Texas 77079 (281) 558-8338 Room Rate: $125.00/night Workshops $45.00 for 1/2 day From kdwyer3322 <@t> aol.com Sat Jan 30 12:24:39 2010 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sat Jan 30 12:24:52 2010 Subject: [Histonet] TEXAS SOCIETY FOR HISTOTECHNOLOGY 2010 MEETING Message-ID: <8CC6FF09E5F73FC-26F0-D707@webmail-d008.sysops.aol.com> All Histonetters: The program is complete for the Texas Society for Histotechnology 2010 State Meeting in Houston Texas. If you would like an electronic copy of the program please contact me via this e-mail. All meeting and workshop information should be posted on the website shortly. Regards, TSH Convention Committee Texas Society for Histotechnology, Inc. 33rdAnnual Symposium/Convention ?Histology: A Kaleidoscope of Change Omni Houston Hotel Westside 13210 Katy Freeway Houston, Texas 77079 (281) 558-8338 Room Rate: $125.00/night Workshops $45.00 for 1/2 day From aazath <@t> hotmail.com Sat Jan 30 23:05:30 2010 From: aazath <@t> hotmail.com (Aazath Raj) Date: Sat Jan 30 23:05:35 2010 Subject: [Histonet] Fading of Histology slides In-Reply-To: References: Message-ID: Dear Colleagues , I am an Histopathology Technologist .We use DPX (merck) as the mounting medium for the the light microscopy slides. Particularly H&E slides gets faded very fast with min 3 -4 month. Is there any other alternative other than gum dammar which we are not getting now a days in the market. Can any one suggest me with some way to avoid the fading of H&E slides. With regards, Aazathraj.P Technical Officer, Department of Histopathology and Cytology Apollo Hospitals-Chennai India _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. https://signup.live.com/signup.aspx?id=60969 From max_histo_00 <@t> yahoo.it Sun Jan 31 02:44:06 2010 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Sun Jan 31 02:44:21 2010 Subject: R: [Histonet] honing compound In-Reply-To: Message-ID: <360211.50549.qm@web23305.mail.ird.yahoo.com> Hi, Jennifer, try these: http://stores.shop.ebay.it/SharkDesigns-uk-Bushcraft__W0QQ_armrsZ1 http://stores.shop.ebay.it/Sandpaper-and-Computer-and-Parts__W0QQ_armrsZ1 They are both coming from GB. I bought from them an Arkansas stone, a strop and its paste, and some sandpaper sheets with thin and thinest grid up to 1 micron. Best Regards, Massimo Tosi ================================================= In ricordo di "Nice": "E Argo, che aveva visto Odisseo dopo vent?anni, fu preso dal Fato della nera morte." Omero, Odissea, XVII, 290-327 --- Ven 29/1/10, Jennifer MacDonald ha scritto: Da: Jennifer MacDonald Oggetto: [Histonet] honing compound A: histonet@lists.utsouthwestern.edu Data: Venerd? 29 gennaio 2010, 07:03 A colleague from a neighboring university is trying to find both fine and coarse honing compound for his knife sharpener.? Does anyone have a good source? Thank you, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Sun Jan 31 13:01:46 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Sun Jan 31 13:01:51 2010 Subject: [Histonet] Pathologist Assistant Job Opportunity in TN Message-ID: <1759022405.1264964505856.JavaMail.cfservice@webserver53> Dear Pathologist Assistants, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Laboratory Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory positions across the country. Our clients typically assist with relocation expenses. One particular client I am working with is one of the top hospital systems in the country. This hospital in Tennessee is looking for a Pathologist Assistant for their Pathology Department. My client is looking for someone who is PA(ASCP) certified and has at least 1 year experience as a pathologist assistant. This position has a very competitive base salary, great benefits, and an unparalleled retirement package. My client will also assist with relocation expenses. If you're interested in learning more about this opportunity or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Laboratory positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From rjbuesa <@t> yahoo.com Sun Jan 31 14:13:15 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jan 31 14:13:20 2010 Subject: [Histonet] Fading of Histology slides In-Reply-To: Message-ID: <417860.69066.qm@web65715.mail.ac4.yahoo.com> Almost any kind of mounting medium can present some fading IF the slides are not properly prepared before coverslipping or IF the medium is more acid that it should or IF the slides are exposed to strong light. You have to consider all these 3 factors before trying to switch to a different mounting medium as a solution to your problem. Oven drying your sections before coverslipping eliminates many of these fading conditions. Ren? J. ? --- On Sun, 1/31/10, Aazath Raj wrote: From: Aazath Raj Subject: [Histonet] Fading of Histology slides To: histonet@lists.utsouthwestern.edu Date: Sunday, January 31, 2010, 12:05 AM Dear Colleagues , ? ? ? ? ? ? ? ? ? ? I am an Histopathology Technologist .We use DPX (merck) as the mounting medium for the the light microscopy slides. Particularly H&E slides gets faded very fast with min 3 -4 month. Is there any other alternative other than gum dammar which we are not getting now a days in the market. Can any one suggest me with some way to avoid the fading of H&E slides. With regards, Aazathraj.P Technical Officer, Department of Histopathology and Cytology Apollo Hospitals-Chennai India ??? ???????? ?????? ??? ? _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. https://signup.live.com/signup.aspx?id=60969_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet