From jkiernan <@t> uwo.ca Mon Feb 1 00:08:11 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Feb 1 00:08:16 2010 Subject: [Histonet] Klotz fixative Message-ID: A Google search for KLOTZ FIXATIVE brings up many hits, with indications that "Klotz solution" is an embalming fluid for gross anatomy. That's not the same as as a fixative. Follow up the links and see if they match what you need. Embalming fluids do not necessarily provide good histological fixation. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Perry, Margaret" Date: Friday, January 29, 2010 16:00 Subject: [Histonet] Klotz fixative To: "histonet@lists.utsouthwestern.edu" > > Do you know of anyone that sells Klotz fixative? We use it > in a pre vet class but would like to eliminate the > choloralhydrate since it is such a hassel due to it's being a > controlled substance. Is there a substitute fixative that would > retain the properties of Klotz? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Mon Feb 1 07:23:52 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Feb 1 07:23:59 2010 Subject: [Histonet] Questions for a Pathology Candidate Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46E33@nmdamailsvr.nmda.ad.nmsu.edu> I have pondered and edited the answers I've gotten from many of you and, in response to some very pointed suggestions that EVERYTHING be shared on Histonet to further communication and professional "bonding", here are the questions for the Hypothetical Interview of a Pathologist: 1. What is the role of the histologist to you in your personal profession position? 2. Do you consider the histologist a part of the professional staff? 3. How much time have you spent in a histology lab, observing or performing histology tasks? Have you processed (cassetting to staining) samples to be aware of the effects of collection? 4. Have you performed routine H&E, special stains and IHC? 5. What are your favorite special stains? 6. How would you handle a suggestion or comment from a histologist regarding technique? 7. How would you address a problem with a section or a stain with the histologist? 8. What would a histologist from a former workplace say about you? 9. What is your communication style? 10. What are your TAT expectations - surgicals, special stains, IHC, autopsies/necropsies, etc.? 11. What, in general, are your expectations of the histology laboratory? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From flnails <@t> texaschildrens.org Mon Feb 1 07:44:52 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon Feb 1 07:45:20 2010 Subject: [Histonet] TEXAS SOCIETY FOR HISTOTECHNOLOGY 2010 MEETING In-Reply-To: <8CC6FF062AFC0C9-26F0-D6A2@webmail-d008.sysops.aol.com> References: <8CC6FF062AFC0C9-26F0-D6A2@webmail-d008.sysops.aol.com> Message-ID: Please forward me a copy. Thanks, -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kdwyer3322@aol.com Sent: Saturday, January 30, 2010 12:23 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] TEXAS SOCIETY FOR HISTOTECHNOLOGY 2010 MEETING All Histonetters: The program is complete for the Texas Society for Histotechnology 2010 State Meeting in Houston Texas. If you would like an electronic copy of the program please contact me via this e-mail. All meeting and workshop information should be posted on the website shortly. Regards, TSH Convention Committee Texas Society for Histotechnology, Inc. 33rdAnnual Symposium/Convention "Histology: A Kaleidoscope of Change Omni Houston Hotel Westside 13210 Katy Freeway Houston, Texas 77079 (281) 558-8338 Room Rate: $125.00/night Workshops $45.00 for 1/2 day _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From gvdobbin <@t> ihis.org Mon Feb 1 08:18:45 2010 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Feb 1 08:19:16 2010 Subject: [Histonet] Klotz fixative Message-ID: The benefit of Klotz is it preserves tissues/organs while not altering the color and texture "much". Therefore good for demonstration labs. We used to store teaching specimens in Klotz in the walk-in cooler (+ 4C) to aid in the preservation even further. It is not adequate for long-term storage as far as I am aware. I have no experience with the affects on histological preparations. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> John Kiernan 2/1/2010 2:08 AM >>> A Google search for KLOTZ FIXATIVE brings up many hits, with indications that "Klotz solution" is an embalming fluid for gross anatomy. That's not the same as as a fixative. Follow up the links and see if they match what you need. Embalming fluids do not necessarily provide good histological fixation. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Perry, Margaret" Date: Friday, January 29, 2010 16:00 Subject: [Histonet] Klotz fixative To: "histonet@lists.utsouthwestern.edu" > > Do you know of anyone that sells Klotz fixative? We use it > in a pre vet class but would like to eliminate the > choloralhydrate since it is such a hassel due to it's being a > controlled substance. Is there a substitute fixative that would > retain the properties of Klotz? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From EdieL <@t> fmchealth.org Mon Feb 1 08:23:18 2010 From: EdieL <@t> fmchealth.org (Edie Lehman) Date: Mon Feb 1 08:23:26 2010 Subject: [Histonet] Steel microtome knives Message-ID: <37BC1F8B840D1F40A11EE5F7F362E5CE0466B226E5@EX07.fmchealth.org> While doing some major cleaning, we found 14 of the old steel microtome knives (the kind that need to be sharpened), all in cases. Also 5 honing plates. Is anyone interested in these? Pay for shipping and they are yours! Edie Lehman MT(ASCP) Anatomical Pathology Supervisor EdieL@fmchealth.org (740)687-8807 * Please consider the environment before printing this email Fairfield Medical Center People you know. Care you trust. Visit us at http://www.fmchealth.org or our online store at http://fairfield.thehospitalstore.com "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From Cathy.Crumpton <@t> tuality.org Mon Feb 1 10:39:44 2010 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Mon Feb 1 10:40:18 2010 Subject: [Histonet] Tape/Film Coverslips vs. Glass Message-ID: Hello all, We mig to get feedb and tape coverslips histology and cytology?&nbs and most of the responses were dated about the tape systems since then? receiving your input. Thanks, Cath From gayle.callis <@t> bresnan.net Mon Feb 1 11:19:06 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Feb 1 11:19:25 2010 Subject: [Histonet] Comparison of AEC chromogens Message-ID: <000401caa362$a9e46300$fdad2900$@callis@bresnan.net> Dear All, How does DAKO AEC+ compare to the new immPACT peroxidase substrate (AEC) from Vector in terms of sensitivity, or as Dr. van der Loos would say, efficiency? Is your primary antibody concentration comparatively the same with both of these AEC products? We have enjoyed excellent results with AEC+ over the years or with van der Loos AEC recipe- allowing low antibody concentrations with our murine CD markers. We are not interested in DAB since we prefer a tinctorial red color of the AEC(cells) with blue(nuclei/tissue) for contrast. We are always interested in trying new chromogen kits available on the market. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From srishan <@t> mail.holyname.org Mon Feb 1 12:11:20 2010 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Mon Feb 1 12:11:47 2010 Subject: [Histonet] RUO AND ASR ANTIBODIES Message-ID: Ventana Users, Need some advise regarding the RUO and ASR antibodies. Is anyone out there in a hospital setting using RUO and ASR reagents with the Ventana XT? If so, is it necessary to add a disclaimer on the reports? Are they chargable? Are you using IVD antibodies from other vendors? The antibodies under consideration are p63 and mammoglobin. Thanks in advance!! Nirmala Srishan Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare , 2009 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From talulahgosh <@t> gmail.com Mon Feb 1 12:17:56 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Feb 1 12:18:00 2010 Subject: [Histonet] fluorescence with xylene dehydration Message-ID: Hello Has anyone used a xylene dehydration at the end of fluorescent staining? I've searched the histonet archives for this answer but the answers were mixed. I thought I'd ask again to see if there were any new opinions. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum From Maxim_71 <@t> mail.ru Mon Feb 1 13:46:38 2010 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Mon Feb 1 13:46:48 2010 Subject: [Histonet] Microtomist's Vade-Mecum on the Web Message-ID: <735154512.20100201224638@mail.ru> Dear Histonetters! You can read online many old excellent microtechnique textbooks here: www.archive.org. A. Boiles-Lee (1904) "Microtomisits Vade-mecum" free available here: http://www.archive.org/details/microtomistsvade00leea You can save it as pdf. It was very important for me, because I have not a possibility to buy it. Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From jkiernan <@t> uwo.ca Mon Feb 1 14:20:34 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Feb 1 14:20:42 2010 Subject: [Histonet] fluorescence with xylene dehydration Message-ID: Yes, I've done this with fluorescein- and rhodamine-labelled protein tracers and lectins. The fluorescence is still there years later, in slides stored in boxes at room temperature. Permanent mounting media are also OK for preparations stained with fluorescent antibodies. See Espada J, Juarranz A, Galaz S, Canete M, Villanueva A, Pecheco M & Stockert JC (2005) "Non-aqueous permanent mounting for immunofluorescence microscopy" Histochem. Cell Biol. 123: 329-334. A Scopus search shows 7 papers citing this one (2006-2009), all in good journals Alcohol dehydration of the sections coagulates the fluorescently labelled protein in situ. After clearing, it's important to use a non-fluorescent resinous mounting medium. In my experience DPX (polystyrene based) is OK and so are at least two of the poly(methylmethacrylate) media, Entellan and Cytoseal. Canada balsam is unsuitable because it fluoresces enough to interfere with interpretation of the slides. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Emily Sours Date: Monday, February 1, 2010 13:19 Subject: [Histonet] fluorescence with xylene dehydration To: histonet@lists.utsouthwestern.edu > Hello > > Has anyone used a xylene dehydration at the end of fluorescent > staining?I've searched the histonet archives for this answer but > the answers were > mixed. I thought I'd ask again to see if there were any > new opinions. > > Emily > > > > ...the thrill of being close to that hidden knowledge. That's > the way I feel > when I read Nabokov. Encrypted within his words, encoded > indecipherably,ambiguously, is the equivalent of the secret of > lightning. Something akin to > the secret code of higher human consciousness, the DNA, the > genome of > genius. > -Ron Rosenbaum > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley <@t> burnham.org Mon Feb 1 14:32:50 2010 From: jshelley <@t> burnham.org (John Shelley) Date: Mon Feb 1 14:32:56 2010 Subject: [Histonet] New Mouse Project Message-ID: Hi Histonetters, I was just asked to start up a new project and was wondering if anyone out there has used these markers for mouse and where do you purchase them from and what type of protocol are you using to get them to work. Here is the list of antibodies any help will be greatly appreciated: CD3, CD4, CD5, CD8, CD10, CD11b, CD20, F4/80 Kind Regards! John From KPercival <@t> wyeth.com Mon Feb 1 14:36:33 2010 From: KPercival <@t> wyeth.com (Percival Karen) Date: Mon Feb 1 14:40:21 2010 Subject: [Histonet] New Mouse Project In-Reply-To: References: Message-ID: <4B66F50202000011001F82E6@gv01a67m.gv.us.pri.wyeth.com> John - FFPE or frozen? I have protocols for most of those. karen >>> John Shelley 2/1/2010 3:32 PM >>> Hi Histonetters, I was just asked to start up a new project and was wondering if anyone out there has used these markers for mouse and where do you purchase them from and what type of protocol are you using to get them to work. Here is the list of antibodies any help will be greatly appreciated: CD3, CD4, CD5, CD8, CD10, CD11b, CD20, F4/80 Kind Regards! John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brian <@t> prometheushealthcare.com Mon Feb 1 15:24:41 2010 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Mon Feb 1 15:24:48 2010 Subject: [Histonet] Day Shift with sign on in Long Island Message-ID: <01af01caa384$f7faf4b0$e7f0de10$@com> Currently working on a day shift in Syosset, NY- competitive pay, sign on , excellent benefits and insurance. Please let me know if you may be interested! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From gayle.callis <@t> bresnan.net Mon Feb 1 15:31:55 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Feb 1 15:32:11 2010 Subject: [Histonet] fluorescence with xylene dehydration In-Reply-To: References: Message-ID: <000501caa385$fb5af320$f210d960$@callis@bresnan.net> It also may depend on the fluorophore you are using (you did not say???) John Kiernan's reply was excellent but if you are using Alexa or Dylight dyes, you should contact the technical services at Molecular Probes for Alexa and whoever makes the Dylight fluorophore since Histonet replies are mixed. Jackson Immunoresearch now supplies antibodies, etc conjugated to various Dylights, and their technical services are excellent for helping you. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Monday, February 01, 2010 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fluorescence with xylene dehydration Hello Has anyone used a xylene dehydration at the end of fluorescent staining? I've searched the histonet archives for this answer but the answers were mixed. I thought I'd ask again to see if there were any new opinions. Emily __________ Information from ESET Smart Security, version of virus signature database 4824 (20100201) __________ The message was checked by ESET Smart Security. http://www.eset.com From srepucci <@t> cellsignal.com Mon Feb 1 15:36:08 2010 From: srepucci <@t> cellsignal.com (Stephen Repucci) Date: Mon Feb 1 15:36:14 2010 Subject: [Histonet] Research Associate job opportunity (Danvers, MA) Message-ID: <8C2B0AEE-49D5-41E9-B148-0464FE8BC06C@cellsignal.com> Cell Signaling Technology, Inc. (CST) is a worldwide leader in the development and commercialization of antibodies and assays for pathway analysis, as well as novel discovery technologies such as PhosphoScan?. We are committed to developing innovative new research tools to help define the mechanisms underlying cell function and disease, thereby broadly accelerating progress in biomedical research and medicine. We are seeking a Research Associate in the Immunohistochemistry Group to assist with the validation of antibodies for use in Immunohistochemistry. Responsibilities will include immunohistochemical analysis on paraffin-embedded tissues and cells, but may evolve as the company continues to grow. Additional responsibilities include protocol optimization/assay development, discovery research, specialized staining projects, and may also include product development. The candidate should be very well organized with exquisite attention to detail and capable of multi- tasking and working independently in a fast paced, dynamic and high- volume testing environment. The ideal candidate will have an MS or BS in a life science with at least two years of general laboratory experience. IHC experience is preferred. For more information please contact me or apply on-line at: https://home.eease.com/recruit/?id=484960 Stephen Repucci srepucci@cellsignal.com 978-867-2318 From gayle.callis <@t> bresnan.net Mon Feb 1 16:08:54 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Feb 1 16:09:11 2010 Subject: [Histonet] Microtomist's Vade-Mecum on the Web In-Reply-To: <735154512.20100201224638@mail.ru> References: <735154512.20100201224638@mail.ru> Message-ID: <000b01caa38b$25d44390$717ccab0$@callis@bresnan.net> Thank you, Maxim Another excellent and still very useful book is the original Animal Tissue Techniques by Gretchen Humason, 1962 edition. Books like this are found in the Biodiversity Heritage Library if you want to narrow your search. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Monday, February 01, 2010 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtomist's Vade-Mecum on the Web Dear Histonetters! You can read online many old excellent microtechnique textbooks here: www.archive.org. A. Boiles-Lee (1904) "Microtomisits Vade-mecum" free available here: http://www.archive.org/details/microtomistsvade00leea You can save it as pdf. It was very important for me, because I have not a possibility to buy it. Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 4824 (20100201) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4825 (20100201) __________ The message was checked by ESET Smart Security. http://www.eset.com From jsantiago <@t> bellsouth.net Mon Feb 1 20:29:02 2010 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Mon Feb 1 20:29:12 2010 Subject: [Histonet] Mississippi Society For Histotechnology Meeting Message-ID: <003f01caa3af$7e9fb300$7bdf1900$@net> Homecoming Mississippi - A New Beginning The program for the upcoming meeting in Mississippi is now available. To access and register online, please visit http://www.nsh.org/content/mississippi-society-histotechnology-homecoming-ev ent Jerry Santiago, BS, HTL(ASCP)QIHC NSH Region III Director From jsantiago <@t> bellsouth.net Mon Feb 1 20:43:31 2010 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Mon Feb 1 20:43:39 2010 Subject: [Histonet] Link correction for the Mississippi meeting Message-ID: <006001caa3b1$84d941d0$8e8bc570$@net> The correct link to download a program and/or register online for the Mississippi Society for Histotechnology Meeting is: http://www.nsh.org/content/mississippi-society-histotechnology-homecoming-ev ent From jsantiago <@t> bellsouth.net Mon Feb 1 20:51:12 2010 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Mon Feb 1 20:51:22 2010 Subject: [Histonet] Mississippi Homecoming Message-ID: <006501caa3b2$974e2eb0$c5ea8c10$@net> Homecoming Mississippi - A New Beginning The program for the upcoming meeting in Mississippi is now available. To access and register online, please visit http://www.nsh.org/content/mississippi-society-histotechnology-homecoming-ev ent Jerry Santiago, BS, HTL(ASCP)QIHC NSH Region III Director From jkiernan <@t> uwo.ca Tue Feb 2 00:21:25 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Feb 2 00:21:32 2010 Subject: [Histonet] Research Associate job opportunity (Danvers, MA) In-Reply-To: <8C2B0AEE-49D5-41E9-B148-0464FE8BC06C@cellsignal.com> References: <8C2B0AEE-49D5-41E9-B148-0464FE8BC06C@cellsignal.com> Message-ID: Why doesn't your job ad say what the salary is? ----- Original Message ----- From: Stephen Repucci Date: Monday, February 1, 2010 16:37 Subject: [Histonet] Research Associate job opportunity (Danvers, MA) To: histonet@lists.utsouthwestern.edu > Cell Signaling Technology, Inc. (CST) is a worldwide leader in > the > development and commercialization of antibodies and assays for > pathway > analysis, as well as novel discovery technologies such as > PhosphoScan?. We are committed to developing innovative new > research > tools to help define the mechanisms underlying cell function > and > disease, thereby broadly accelerating progress in biomedical > research > and medicine. > > We are seeking a Research Associate in the Immunohistochemistry > Group > to assist with the validation of antibodies for use in > Immunohistochemistry. Responsibilities will include > immunohistochemical analysis on paraffin-embedded tissues and > cells, > but may evolve as the company continues to grow. > Additional > responsibilities include protocol optimization/assay > development, > discovery research, specialized staining projects, and may > also > include product development. The candidate should be very > well > organized with exquisite attention to detail and capable of > multi- > tasking and working independently in a fast paced, dynamic and > high- > volume testing environment. The ideal candidate will have an MS > or BS > in a life science with at least two years of general > laboratory > experience. IHC experience is preferred. > > For more information please contact me or apply on-line at: > https://home.eease.com/recruit/?id=484960 > > Stephen Repucci > srepucci@cellsignal.com > 978-867-2318 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Tue Feb 2 05:34:04 2010 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Tue Feb 2 05:37:41 2010 Subject: [Histonet] staining questions Message-ID: Good morning! I will be staining vessels for proteoglycans using Alcian Blue. I was going to use the 2.5pH protocol with a Nuclear Fast Red counterstain. Does anyone have any other suggestion? The investigator does not want Movats. Also, I normally use PTAH as my fibrin stain for clots but was thinking that there may be something better. I found the Fraser-Lendrum but the protocol stated that Zenkers was the best fixative and that is out of the question. So again, any suggestions? Thank you in advance. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From settembr <@t> umdnj.edu Tue Feb 2 07:54:07 2010 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Feb 2 07:54:22 2010 Subject: [Histonet]Vendor for Surfactant Apoprotein Message-ID: Looking for a reliable antibody for immunohistochemistry to run Surfactant Apoprotein on human formalin fixed paraffin embedded tissue? Can you tell me your vendor, please? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Feb 2 07:59:47 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Feb 2 08:02:07 2010 Subject: [Histonet]Vendor for Surfactant Apoprotein In-Reply-To: References: Message-ID: Try Biocare. It works well for us. catalog number CM 275 Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Settembre [settembr@umdnj.edu] Sent: Tuesday, February 02, 2010 8:54 AM To: Betsy Molinari; histonet@lists.utsouthwestern.edu Cc: Catherine Susan Delia; Neena Mirani Subject: [Histonet]Vendor for Surfactant Apoprotein Looking for a reliable antibody for immunohistochemistry to run Surfactant Apoprotein on human formalin fixed paraffin embedded tissue? Can you tell me your vendor, please? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Tue Feb 2 08:46:16 2010 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Tue Feb 2 09:17:59 2010 Subject: FW: [Histonet] Research Associate job opportunity (Danvers, MA) Message-ID: <385004FA0C9C435481D909885CC4CDA4@IBLS.GLA.AC.UK> John, Its science, we do it for love, money is not an issue. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: 02 February 2010 06:21 To: Stephen Repucci Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Research Associate job opportunity (Danvers, MA) Why doesn't your job ad say what the salary is? ----- Original Message ----- From: Stephen Repucci Date: Monday, February 1, 2010 16:37 Subject: [Histonet] Research Associate job opportunity (Danvers, MA) To: histonet@lists.utsouthwestern.edu > Cell Signaling Technology, Inc. (CST) is a worldwide leader in > the > development and commercialization of antibodies and assays for > pathway > analysis, as well as novel discovery technologies such as > PhosphoScanR. We are committed to developing innovative new > research > tools to help define the mechanisms underlying cell function > and > disease, thereby broadly accelerating progress in biomedical > research > and medicine. > > We are seeking a Research Associate in the Immunohistochemistry > Group > to assist with the validation of antibodies for use in > Immunohistochemistry. Responsibilities will include > immunohistochemical analysis on paraffin-embedded tissues and > cells, > but may evolve as the company continues to grow. > Additional > responsibilities include protocol optimization/assay > development, > discovery research, specialized staining projects, and may > also > include product development. The candidate should be very > well > organized with exquisite attention to detail and capable of > multi- > tasking and working independently in a fast paced, dynamic and > high- > volume testing environment. The ideal candidate will have an MS > or BS > in a life science with at least two years of general > laboratory > experience. IHC experience is preferred. > > For more information please contact me or apply on-line at: > https://home.eease.com/recruit/?id=484960 > > Stephen Repucci > srepucci@cellsignal.com > 978-867-2318 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Tue Feb 2 09:24:29 2010 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Feb 2 09:24:36 2010 Subject: [Histonet] Alcohol fixation and immuno Message-ID: <379A927A452F3D43A3C8705F4E67905F0EB9935899@EX05.net.ucsf.edu> Good morning everyone, We occasionally receive specimens that were fixed in alcohol but processed on our machine, which starts in formalin. In our typical antigen retrieval we either use a 95 degree waterbath for an hour or enzyme for 5 minutes. On alcohol fixed tissues this chews up the tissue but we get staining. If we repeat the stains with no retrieval to try to minimize the damage, we get very weak or no staining at all (in a general sense - I'm not speaking about any particular antibody). Is this because we have a small degree of formalin fixation because of the formalin step on the processor? Enough to require some retrieval but not as much as formalin fixed tissue? Has anyone else run into this problem? If so, how did you solve it? Thank you for you help! Erin Martin UCSF Dermatopathology From jsantiago <@t> bellsouth.net Tue Feb 2 09:33:47 2010 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Tue Feb 2 09:40:32 2010 Subject: [Histonet] Mississippi Society for Histotechnology Message-ID: <477257.61268.qm@web180407.mail.gq1.yahoo.com> Dear Histonetters, I am posting the link for the Mississippi meeting. It seems to show as an incomplete link on the histonet. If the link breaks again, plase copy and paste the entire link in your url. If you need a program to be e-mailed or mailed to you, send me an e-mail with the request. http://nsh.org/content/mississippi-society-histotechnology-homecoming-event Jerry Santiago, BS, HTL(ASCP)QIHC NSH Region III Director Shands Jacksonville W: 904-244-6149 From CIngles <@t> uwhealth.org Tue Feb 2 10:51:47 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Feb 2 10:52:52 2010 Subject: [Histonet] Research Associate job opportunity (Danvers, MA) References: <385004FA0C9C435481D909885CC4CDA4@IBLS.GLA.AC.UK> Message-ID: Ian: Love doesn't pay the bills these days. ;) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ian Montgomery Sent: Tue 2/2/2010 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Research Associate job opportunity (Danvers, MA) John, Its science, we do it for love, money is not an issue. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: 02 February 2010 06:21 To: Stephen Repucci Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Research Associate job opportunity (Danvers, MA) Why doesn't your job ad say what the salary is? ----- Original Message ----- From: Stephen Repucci Date: Monday, February 1, 2010 16:37 Subject: [Histonet] Research Associate job opportunity (Danvers, MA) To: histonet@lists.utsouthwestern.edu > Cell Signaling Technology, Inc. (CST) is a worldwide leader in > the > development and commercialization of antibodies and assays for > pathway > analysis, as well as novel discovery technologies such as > PhosphoScanR. We are committed to developing innovative new > research > tools to help define the mechanisms underlying cell function > and > disease, thereby broadly accelerating progress in biomedical > research > and medicine. > > We are seeking a Research Associate in the Immunohistochemistry > Group > to assist with the validation of antibodies for use in > Immunohistochemistry. Responsibilities will include > immunohistochemical analysis on paraffin-embedded tissues and > cells, > but may evolve as the company continues to grow. > Additional > responsibilities include protocol optimization/assay > development, > discovery research, specialized staining projects, and may > also > include product development. The candidate should be very > well > organized with exquisite attention to detail and capable of > multi- > tasking and working independently in a fast paced, dynamic and > high- > volume testing environment. The ideal candidate will have an MS > or BS > in a life science with at least two years of general > laboratory > experience. IHC experience is preferred. > > For more information please contact me or apply on-line at: > https://home.eease.com/recruit/?id=484960 > > Stephen Repucci > srepucci@cellsignal.com > 978-867-2318 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Feb 2 11:25:40 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Feb 2 11:25:49 2010 Subject: [Histonet] Research Associate job opportunity (Danvers, MA) In-Reply-To: Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF779@UWHC-MAIL01.uwhis.hosp.wisc.edu> Touch? Claire. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, February 02, 2010 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Research Associate job opportunity (Danvers, MA) Ian: Love doesn't pay the bills these days. ;) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ian Montgomery Sent: Tue 2/2/2010 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Research Associate job opportunity (Danvers, MA) John, Its science, we do it for love, money is not an issue. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: 02 February 2010 06:21 To: Stephen Repucci Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Research Associate job opportunity (Danvers, MA) Why doesn't your job ad say what the salary is? ----- Original Message ----- From: Stephen Repucci Date: Monday, February 1, 2010 16:37 Subject: [Histonet] Research Associate job opportunity (Danvers, MA) To: histonet@lists.utsouthwestern.edu > Cell Signaling Technology, Inc. (CST) is a worldwide leader in the > development and commercialization of antibodies and assays for > pathway > analysis, as well as novel discovery technologies such as > PhosphoScanR. We are committed to developing innovative new > research > tools to help define the mechanisms underlying cell function > and > disease, thereby broadly accelerating progress in biomedical > research > and medicine. > > We are seeking a Research Associate in the Immunohistochemistry Group > to assist with the validation of antibodies for use in > Immunohistochemistry. Responsibilities will include > immunohistochemical analysis on paraffin-embedded tissues and > cells, > but may evolve as the company continues to grow. > Additional > responsibilities include protocol optimization/assay > development, > discovery research, specialized staining projects, and may > also > include product development. The candidate should be very > well > organized with exquisite attention to detail and capable of > multi- > tasking and working independently in a fast paced, dynamic and > high- > volume testing environment. The ideal candidate will have an MS > or BS > in a life science with at least two years of general > laboratory > experience. IHC experience is preferred. > > For more information please contact me or apply on-line at: > https://home.eease.com/recruit/?id=484960 > > Stephen Repucci > srepucci@cellsignal.com > 978-867-2318 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 2 12:03:17 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 2 12:03:21 2010 Subject: [Histonet] Alcohol fixation and immuno In-Reply-To: <379A927A452F3D43A3C8705F4E67905F0EB9935899@EX05.net.ucsf.edu> Message-ID: <897381.26918.qm@web65712.mail.ac4.yahoo.com> Exactly! Your tissue suffer (that is the appropriate word) a double fixation, probably?partial in? both, but the formalin step will cross link the proteins requiring epitope retrieval. Ren? J. --- On Tue, 2/2/10, Martin, Erin wrote: From: Martin, Erin Subject: [Histonet] Alcohol fixation and immuno To: "histonet" Date: Tuesday, February 2, 2010, 10:24 AM Good morning everyone, We occasionally receive specimens that were fixed in alcohol but processed on our machine, which starts in formalin.? In our typical antigen retrieval we either use a 95 degree waterbath for an hour or enzyme for 5 minutes.? On alcohol fixed tissues this chews up the tissue but we get staining.? If we repeat the stains with no retrieval to try to minimize the damage, we get very weak or no staining at all (in a general sense - I'm not speaking about any particular antibody).? Is this because we have a small degree of formalin fixation because of the formalin step on the processor?? Enough to require some retrieval but not as much as formalin fixed tissue?? Has anyone else run into this problem?? If so, how did you solve it? Thank you for you help! Erin Martin UCSF Dermatopathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Tue Feb 2 12:50:54 2010 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Tue Feb 2 12:51:08 2010 Subject: [Histonet] IHC protocol for vimentin in animal tissue Message-ID: Does anyone out there have an IHC protocol, for Vimentin, in animal tissues, that has been working for them on the Ventana Benchmark XT? I would appreciate any suggestions at this point. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From AHutton <@t> dh.org Tue Feb 2 12:52:25 2010 From: AHutton <@t> dh.org (Hutton, Allison) Date: Tue Feb 2 12:54:09 2010 Subject: [Histonet] Formula 83 Message-ID: <38A56C4F4630D348A50B3720409270870744FF19@dhmail.dhorg.org> For those of you who are using Formula 83, could you please contact me off line. We have completed our demo but still have a few questions. Thanks in advance, Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital 595 W. State St Doylestown, PA 18901 215-345-2264 ahutton@dh.org From shive003 <@t> umn.edu Tue Feb 2 13:16:05 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Feb 2 13:16:08 2010 Subject: [Histonet] IHC protocol for vimentin in animal tissue References: Message-ID: <425A2D4A129B40A3A8A432C390340F0D@auxs.umn.edu> Lynn, I don't know anything about the Ventana Benchmark XT requirements, but I have never had any problem with Vimentin on a Dako Autostainer and also done manually on all sorts of animal tissue - with the exception of mouse. The V9 clone doesn't work on mouse, in my experience. (It'll work on frogs, however; go figure.) My protocol uses Dako Target Retrieval in a Biocare Decloaking Chamber for antigen retrieval, 45 min in primary antibody, 30 min in EnVision+/HRP goat anti-mouse IgG, and 10 minutes in AEC. Very strong staining. Hope you get the specific answers you need from others. Jan Shivers UMN Vet Diag Lab ----- Original Message ----- From: "Burton, Lynn" To: Sent: Tuesday, February 02, 2010 12:50 PM Subject: [Histonet] IHC protocol for vimentin in animal tissue Does anyone out there have an IHC protocol, for Vimentin, in animal tissues, that has been working for them on the Ventana Benchmark XT? I would appreciate any suggestions at this point. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Tue Feb 2 14:03:27 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Feb 2 14:03:49 2010 Subject: [Histonet] Alcohol fixation and immuno In-Reply-To: <897381.26918.qm@web65712.mail.ac4.yahoo.com> References: <379A927A452F3D43A3C8705F4E67905F0EB9935899@EX05.net.ucsf.edu> <897381.26918.qm@web65712.mail.ac4.yahoo.com> Message-ID: <4B683EBF.59CD.00EE.0@hurleymc.com> Normally, we used regular charged slides, but we found some slides from Surgipath to help with those "hard to stay on" type tissues. They are called "Apex", Superior Adhesive Slides. Product # 00084. They are frosted on one end, and come in different colors. That product # is Pink. They are not always 100%, but nothing is! A challenging but wonderful field! hope this helps, Lynette >>> Rene J Buesa 2/2/2010 1:03 PM >>> Exactly! Your tissue suffer (that is the appropriate word) a double fixation, probably partial in both, but the formalin step will cross link the proteins requiring epitope retrieval. Ren? J. --- On Tue, 2/2/10, Martin, Erin wrote: From: Martin, Erin Subject: [Histonet] Alcohol fixation and immuno To: "histonet" Date: Tuesday, February 2, 2010, 10:24 AM Good morning everyone, We occasionally receive specimens that were fixed in alcohol but processed on our machine, which starts in formalin. In our typical antigen retrieval we either use a 95 degree waterbath for an hour or enzyme for 5 minutes. On alcohol fixed tissues this chews up the tissue but we get staining. If we repeat the stains with no retrieval to try to minimize the damage, we get very weak or no staining at all (in a general sense - I'm not speaking about any particular antibody). Is this because we have a small degree of formalin fixation because of the formalin step on the processor? Enough to require some retrieval but not as much as formalin fixed tissue? Has anyone else run into this problem? If so, how did you solve it? Thank you for you help! Erin Martin UCSF Dermatopathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Tue Feb 2 14:38:46 2010 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Tue Feb 2 14:39:10 2010 Subject: [Histonet] Betsy - stain/vessels Message-ID: <002f01caa447$b7bcafb0$c5d76880@vetmed.wisc.edu> Betsy, For the Alcian Blue, consider using 1% Alcohol based Eosin for 30 seconds as the counter-stain. I have used the Lendrum's Stain ( on teeth) without Zenker's fixation with favorable results! Vicki From mtitford <@t> aol.com Tue Feb 2 14:52:32 2010 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Tue Feb 2 14:53:21 2010 Subject: [Histonet] Microarray questions Message-ID: <8CC7260C6798B26-4BC8-1634@webmail-d094.sysops.aol.com> We are thinking of making our own microarray blocks instead of purchasing someone elses slides. Can someone: 1) Recommend a punch to use? 2) Which size diameter tip is best? (for general use) 3) are the tips re-usable, or are they single use? I attended April Watanabes's workshop on microarray preparation at the Fort Lauderdale meeting but the equipment discussed in that workshop was Cadillac, where I need more "life in the trenches" type equipment. Thank you in advance. Michael Titford USA Pathology Mobile AL USA From amrithraj_nair <@t> merck.com Tue Feb 2 15:52:41 2010 From: amrithraj_nair <@t> merck.com (Nair, Amrithraj M) Date: Tue Feb 2 15:52:46 2010 Subject: [Histonet] a-2 microglobulin rat Message-ID: I saw that you were successful with HYB339-01 for IHC of a2-microglobulin on FFPE sections of Rat kidney. Would you be kind to share the Antigen retrieval and the staining protocol. Thanks, Amrith ----------------------------------------------------- Amrithraj M. Nair B.V.Sc. & A.H., Ph.D h Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From laura_conner <@t> hotmail.com Tue Feb 2 16:28:18 2010 From: laura_conner <@t> hotmail.com (Laura Conner) Date: Tue Feb 2 16:28:23 2010 Subject: [Histonet] eye fixative recipe ROP Message-ID: Hi everybody. I've been a lurker to this list for awhile and have already learned so much. I'm a total newbie to histology and have much to learn and am so happy to learn from all of you who have so much experience. I'm wondering if anybody has a fixative recipe for eye globes cut into half? I'm wondering if there's a fixative that will reduce retina detachment? Thanks in advance for any help! Laura _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/ From saby_joseph_a <@t> yahoo.com Tue Feb 2 17:27:39 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Tue Feb 2 17:27:44 2010 Subject: [Histonet] staining questions In-Reply-To: References: Message-ID: <801100.74937.qm@web113818.mail.gq1.yahoo.com> Betsy- When staining for proteoglycans, a stain usually associated with Alcian Blue would be PAS.? Stain with Alcian Blue first, then perform the PAS.? A light hematoxylin usually completes the stain. Joe Saby, BA HT ________________________________ From: "Molinari, Betsy" To: histonet@lists.utsouthwestern.edu Sent: Tue, February 2, 2010 6:34:04 AM Subject: [Histonet] staining questions Good morning! I will be staining vessels for proteoglycans using Alcian Blue. I was going to use the 2.5pH? protocol with a Nuclear Fast Red counterstain. Does anyone have any other suggestion? The investigator does not want Movats. Also, I normally use PTAH as my fibrin stain for clots but was thinking that there may be something better. I found the Fraser-Lendrum but the protocol stated that Zenkers was the best fixative and that is out of the question. So again, any suggestions? Thank you in advance. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ppinchr <@t> yahoo.com Tue Feb 2 18:08:43 2010 From: ppinchr <@t> yahoo.com (RP) Date: Tue Feb 2 18:08:47 2010 Subject: [Histonet] microwave processor Message-ID: <308162.92401.qm@web37102.mail.mud.yahoo.com> Hello All, ? I have a dilemma and was hoping you guys could help me. I just took on a part time job in a small GI lab and the previous tech working there left without no one knowing how to use the microwave processor and there's no procedure. I was wondering if anyone has used the Lab pulse Microwave Tissue Processor H 2850 by EBSciences and if there is a procedure for small BX's. I see nothing but magnets on the side and there is no letters or numbers indicating where the magnets are placed. Also I've never used one before. From Joyce.Kwan <@t> elan.com Tue Feb 2 20:19:50 2010 From: Joyce.Kwan <@t> elan.com (Kwan, Joyce) Date: Tue Feb 2 20:19:58 2010 Subject: [Histonet] M-1 embedding matrix question Message-ID: <64320CF42E347D4E8A1EC3D2CB26B6D514370D@PHXEXCMB-PN03.ecorp.egn> Hello Histonetters, I am using Shandon M-1 embedding matrix for cryosectioning perfused, cryo-protected spinal cord. The embedding media allows me to section without the curling you frequently get with OCT compound. One thing I am noticing, however, is the M-1 media does not dissolve very well, even in several washes in water/PBS, and dehydrations in ethanol and xylene. Consequently, the M-1 residue stains along with the histological stain. OCT dissolves cleanly. Have any of you experienced this, or have any suggestions on how to avoid this problem? The M-1 media is a new bottle (will not expire until Dec, 2011). Thank you in advanced for your suggestions. Best regards, Joyce Kwan Elan Pharmaceuticals South San Francisco, CA ******************************************************** This communication and any files transmitted with it may contain information that is confidential, privileged and exempt from disclosure under applicable law. It is intended solely for the use of the individual or entity to which it is addressed. If you are not the intended recipient, you are hereby notified that any use, dissemination or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender. Thank you for your co-operation. ******************************************************** From lscott <@t> sfcn.org Tue Feb 2 22:09:45 2010 From: lscott <@t> sfcn.org (Scott Hendricksen) Date: Tue Feb 2 22:09:50 2010 Subject: [Histonet] Tips for drying slides before H&E stain. Message-ID: <6FE94FC0-EAF3-4701-A9F5-DA4147053503@sfcn.org> Hi, Our lab just received a slide drying oven, and we are trying to figure out what's a good temperature for the slides to be heated and for how long. We are mainly doing H&E slides. The way we are currently drying them is under a small fan, then heat them up, and back to the fan. Then finally heating them up and off to the stainer. Just putting them in the slide drying oven for 10 min at 80C melts the paraffin, but leaves some water on the bottom of some sections. Any suggestions to get the slides dry asap ? Also is the oven much help to your labs? Thanks ! Scott Hendricksen HT(ASCP) From lscott <@t> sfcn.org Tue Feb 2 22:15:46 2010 From: lscott <@t> sfcn.org (Scott Hendricksen) Date: Tue Feb 2 22:15:52 2010 Subject: [Histonet] Eosin leaching out of sections Message-ID: <2A0766BB-A879-4A90-B38B-A0C02FEB52BE@sfcn.org> Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) From AnthonyH <@t> chw.edu.au Tue Feb 2 22:19:17 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Feb 2 22:19:29 2010 Subject: [Histonet] Tips for drying slides before H&E stain. In-Reply-To: <6FE94FC0-EAF3-4701-A9F5-DA4147053503@sfcn.org> Message-ID: I would suggest 65oC for 20-25 minutes. High temperature drying will affect IPX results and, especially with under-fixed tissues, adversely affect H&E staining. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: Wednesday, 3 February 2010 3:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Tips for drying slides before H&E stain. Hi, Our lab just received a slide drying oven, and we are trying to figure out what's a good temperature for the slides to be heated and for how long. We are mainly doing H&E slides. The way we are currently drying them is under a small fan, then heat them up, and back to the fan. Then finally heating them up and off to the stainer. Just putting them in the slide drying oven for 10 min at 80C melts the paraffin, but leaves some water on the bottom of some sections. Any suggestions to get the slides dry asap ? Also is the oven much help to your labs? Thanks ! Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From histonet.nospam <@t> vneubert.com Wed Feb 3 01:08:18 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Feb 3 01:08:23 2010 Subject: [Histonet] Tips for drying slides before H&E stain. In-Reply-To: <6FE94FC0-EAF3-4701-A9F5-DA4147053503@sfcn.org> References: <6FE94FC0-EAF3-4701-A9F5-DA4147053503@sfcn.org> Message-ID: <4B6920E2.9060805@vneubert.com> 58?C for 45-60min, slides in an upright position. From jaylundgren <@t> gmail.com Wed Feb 3 05:06:34 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Feb 3 05:06:40 2010 Subject: [Histonet] Alcohol fixation and immuno In-Reply-To: <4B683EBF.59CD.00EE.0@hurleymc.com> References: <379A927A452F3D43A3C8705F4E67905F0EB9935899@EX05.net.ucsf.edu> <897381.26918.qm@web65712.mail.ac4.yahoo.com> <4B683EBF.59CD.00EE.0@hurleymc.com> Message-ID: You did not specify which enzyme you were using, but I am assuming proteinase K. If an hour's retrieval is too much, and no retrieval is too little, I would suggest running some (alcohol fixed) controls at 15, 30, and 45 minutes in the 95C, or 1,2,3, and 4 minutes in the enzyme. Evaluate the slides with a pathologist for staining vs. section adhesion. Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Wed Feb 3 05:33:49 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Wed Feb 3 05:34:24 2010 Subject: [Histonet] microwave processor In-Reply-To: <308162.92401.qm@web37102.mail.mud.yahoo.com> References: <308162.92401.qm@web37102.mail.mud.yahoo.com> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8CBD3569D@EXC-MBX3.cfs.le.ac.uk> I cannot help you with your specific enquiry, re the microwave processor, but gently suggest you learn from this experience and keep a comprehensive record of methods/techniques used in your laboratory, good luck anyway........ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RP Sent: 03 February 2010 00:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processor Hello All, ? I have a dilemma and was hoping you guys could help me. I just took on a part time job in a small GI lab and the previous tech working there left without no one knowing how to use the microwave processor and there's no procedure. I was wondering if anyone has used the Lab pulse Microwave Tissue Processor H 2850 by EBSciences and if there is a procedure for small BX's. I see nothing but magnets on the side and there is no letters or numbers indicating where the magnets are placed. Also I've never used one before. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kjones <@t> upei.ca Wed Feb 3 06:24:37 2010 From: Kjones <@t> upei.ca (Kathleen Jones) Date: Wed Feb 3 06:24:48 2010 Subject: [Histonet] IHC protocol for vimentin in animal tissue Message-ID: <4B6932C50200008B0001DE7D@grpwise.novell.upei.ca> Hello Lynn I work in a veterinary institution and use a Benchmark. Our vimentin works very well. Our protocol is stdCC1,16m. Good luck! Kathy Kathleen Jones Research Technician Pathology/Microbiology AVC - UPEI (902)566-0595 From lscott <@t> sfcn.org Wed Feb 3 07:48:37 2010 From: lscott <@t> sfcn.org (Scott Hendricksen) Date: Wed Feb 3 07:48:43 2010 Subject: [Histonet] Eosin leaching out of sections Message-ID: Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) From noreply <@t> badoo.com Wed Feb 3 07:59:39 2010 From: noreply <@t> badoo.com (Badoo) Date: Wed Feb 3 07:59:44 2010 Subject: [Histonet] =?utf-8?q?Veronica_Cede=C3=B1o_te_ha_dejado_un_mensaj?= =?utf-8?b?ZS4uLg==?= Message-ID: Veronica Cede?o te ha dejado un mensaje... El mensaje y la persona que lo envi? solo te ser? mostrado a ti y borrarlo en cualquier momento. Puedes responder a trav?s del sistema de intercambio de mensajes. Para descubrir qui?n te escribi?, sigue el siguiente link: http://us1.badoo.com/01107496637/in/9.VDNlA6QFg/?lang_id=7 M?s gente que tambi?n te est? esperando: FRANKLIN XAVIER (Portoviejo, Ecuador) Kritos beauty (Portoviejo, Ecuador) Xavier (Portoviejo, Ecuador) http://us1.badoo.com/01107496637/in/9.VDNlA6QFg/?lang_id=7 Si al pulsar el enlace de este mensaje no funciona, copia y p?galo en la barra de tu navegador. Este email es parte del procedimiento del sistema para el env?o de mensajes enviados por Veronica Cede?o. Si has recibido este mensaje por error, ignora este email. Tras un corto periodo de tiempo, ser? eliminado del sistema. ?Div?rtete! El equipo de Badoo Has recibido este email porque un usuario de Badoo te ha dejado un mensaje en Badoo. Este mensaje es autom?tico. Las respuestas a este mensaje no estan controladas y no ser?n contestadas. Si no quieres recibir m?s mensajes de Badoo, h?znoslo saber: http://us1.badoo.com/impersonation.phtml?lang_id=7&mail_code=21&email=histonet%40lists.utsouthwestern.edu&secret=&invite_id=411714&user_id=1107496637 From lscott <@t> sfcn.org Wed Feb 3 08:02:21 2010 From: lscott <@t> sfcn.org (Scott Hendricksen) Date: Wed Feb 3 08:02:26 2010 Subject: [Histonet] Eosin leaching out of sections References: Message-ID: <777CB212-5D41-4EC3-B7E3-7F7294B60A52@sfcn.org> > Hi again, > > Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? > > Thanks, > > Scott Hendricksen HT(ASCP) From Janet.Bonner <@t> FLHOSP.ORG Wed Feb 3 08:10:04 2010 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed Feb 3 08:11:07 2010 Subject: [Histonet] Eosin leaching out of sections References: <777CB212-5D41-4EC3-B7E3-7F7294B60A52@sfcn.org> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2CA3@fhosxchmb006.ADVENTISTCORP.NET> Your xylene and/or alcohols may need changing. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Scott Hendricksen Sent: Wed 2/3/2010 9:02 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections > Hi again, > > Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? > > Thanks, > > Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From cpyse <@t> x-celllab.com Wed Feb 3 08:21:15 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Feb 3 08:23:44 2010 Subject: [Histonet] Tape/Film Coverslips vs. Glass In-Reply-To: References: Message-ID: <000001caa4dc$25705680$70510380$@com> Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy.Crumpton@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?&nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plambert <@t> wisc.edu Wed Feb 3 09:12:25 2010 From: plambert <@t> wisc.edu (Paul Lambert) Date: Wed Feb 3 09:12:34 2010 Subject: [Histonet] thermal printer microscope labels Message-ID: <506BFD4C-63B0-4DC7-9411-B3B40EF18E44@wisc.edu> Does anyone have experience with printed plastic labels for microscope slides that are claimed to be resistant to various solvents and chemical used in histolology? If so what product do you use? One company (General Data) apparently has a special version of their label that is compatible with heat-based antigen unmasking steps... Any experience there as well? Appreciate the feedback Thanks Paul F. Lambert, Ph.D. Professor of Oncology McArdle Laboratory for Cancer Research University of Wisconsin School of Medicine and Public Health 1400 University Ave. Madison WI 54706 USA tel: 608-262-8533 fax: 608-262-2824 email: plambert@wisc.edu From sjkitten <@t> live.com Wed Feb 3 09:54:02 2010 From: sjkitten <@t> live.com (S R) Date: Wed Feb 3 09:54:09 2010 Subject: [Histonet] Microtome PM Message-ID: Hello Everyone. Does anyone know if there is anyone in the northeast that does microtome pm's? The microtome is a Microm Hm 315. I do not think it has ever had a pm and it is starting to act up. Thanks in Advanced. Sammy _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/ From mucram11 <@t> comcast.net Wed Feb 3 10:15:15 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Wed Feb 3 10:15:19 2010 Subject: [Histonet] Tape/Film Coverslips vs. Glass In-Reply-To: <000001caa4dc$25705680$70510380$@com> Message-ID: <1730070787.402131265213715614.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Glass is generally better for photomicrotomy also.? Pam Marcum UAMS ----- Original Message ----- From: "Cynthia Pyse" To: "Cathy Crumpton" , histonet@lists.utsouthwestern.edu Sent: Wednesday, February 3, 2010 8:21:15 AM GMT -06:00 US/Canada Central Subject: RE: [Histonet] Tape/Film Coverslips vs. Glass Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy.Crumpton@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass ?? Hello all, ?? We mig=t be getting a new coverslipper and the pathologists asked me ?? to ?get feedb=ck from my fellow histotechs that have used both glass ?? and ?tape ?coverslips=. ? Which ?type ?is ?most prefered for general ?? histology ?and ?cytology?&nbs=p; I did some research in the archives ?? and ?most ?of ?the ?responses ?were dated=004. ?Has anything changed ?? about ?the ?tape ?systems ?since ?then? ? =I ?would be interested in ?? receiving your input. ?? ?? Thanks, ?? Cath= ?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Wed Feb 3 10:16:15 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Wed Feb 3 10:16:20 2010 Subject: [Histonet] Microtome PM In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1384B3E6CC@NADCWPMSGCMS03.hca.corpad.net> Sammy, Try Michael Dietrich at Southeast Pathology Instrument Services, Inc at 843-588-2559. I don't know how far north he is willing to go but he is the best in the business!!!!! You can tell him Wanda said so!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of S R Sent: Wednesday, February 03, 2010 10:54 AM To: histo net Subject: [Histonet] Microtome PM Hello Everyone. Does anyone know if there is anyone in the northeast that does microtome pm's? The microtome is a Microm Hm 315. I do not think it has ever had a pm and it is starting to act up. Thanks in Advanced. Sammy _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Wed Feb 3 10:11:35 2010 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Feb 3 10:21:04 2010 Subject: FW: [Histonet] Eosin leaching out of sections Message-ID: Scott, Did you mount from water or a clearing agent? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Feb 3 10:09:03 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Feb 3 10:27:46 2010 Subject: [Histonet] Microtome PM In-Reply-To: Message-ID: You can contact Belair in NJ at 800-783-9424 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of S R Sent: Wednesday, February 03, 2010 10:54 AM To: histo net Subject: [Histonet] Microtome PM Hello Everyone. Does anyone know if there is anyone in the northeast that does microtome pm's? The microtome is a Microm Hm 315. I do not think it has ever had a pm and it is starting to act up. Thanks in Advanced. Sammy _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Dorothy.L.Webb <@t> HealthPartners.Com Wed Feb 3 10:34:09 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Feb 3 10:34:17 2010 Subject: [Histonet] labels Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C56FA39976@HPEMX3.HealthPartners.int> We use the labels from General Data through Dako and are happy with the printing and the resistance to solutions. They adhere well and go through all types of staining from H&E to IHC and are placed in the slide dryer. We have treid both the flapless labels and those with the protective "flaps". Dorotohy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From mike <@t> pathview.com Wed Feb 3 11:54:38 2010 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Feb 3 11:55:14 2010 Subject: [Histonet] thermal printer microscope labels In-Reply-To: <506BFD4C-63B0-4DC7-9411-B3B40EF18E44@wisc.edu> References: <506BFD4C-63B0-4DC7-9411-B3B40EF18E44@wisc.edu> Message-ID: <03b801caa4f9$f9f1dd30$edd59790$@com> The key thing to note with slide labels is that it's not JUST the label. It's the combination of the label, ribbon, and printer. As an LIS vendor, I've heard very, very good things about General Data, and I do know that GD will sell you the label, ribbon, printer and even the bar code scanner. If you have any further questions, feel free to email me. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Lambert Sent: Wednesday, February 03, 2010 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermal printer microscope labels Does anyone have experience with printed plastic labels for microscope slides that are claimed to be resistant to various solvents and chemical used in histolology? If so what product do you use? One company (General Data) apparently has a special version of their label that is compatible with heat-based antigen unmasking steps... Any experience there as well? Appreciate the feedback Thanks Paul F. Lambert, Ph.D. Professor of Oncology McArdle Laboratory for Cancer Research University of Wisconsin School of Medicine and Public Health 1400 University Ave. Madison WI 54706 USA tel: 608-262-8533 fax: 608-262-2824 email: plambert@wisc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Wed Feb 3 11:59:45 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Feb 3 11:59:49 2010 Subject: [Histonet] thermal printer microscope labels In-Reply-To: <03b801caa4f9$f9f1dd30$edd59790$@com> References: <506BFD4C-63B0-4DC7-9411-B3B40EF18E44@wisc.edu> <03b801caa4f9$f9f1dd30$edd59790$@com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D836CF@EMAIL.archildrens.org> Just remember that some thermal labels will fade over time. Be sure your label will still be legible in 10 years. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, February 03, 2010 11:55 AM To: 'Paul Lambert'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] thermal printer microscope labels The key thing to note with slide labels is that it's not JUST the label. It's the combination of the label, ribbon, and printer. As an LIS vendor, I've heard very, very good things about General Data, and I do know that GD will sell you the label, ribbon, printer and even the bar code scanner. If you have any further questions, feel free to email me. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Lambert Sent: Wednesday, February 03, 2010 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermal printer microscope labels Does anyone have experience with printed plastic labels for microscope slides that are claimed to be resistant to various solvents and chemical used in histolology? If so what product do you use? One company (General Data) apparently has a special version of their label that is compatible with heat-based antigen unmasking steps... Any experience there as well? Appreciate the feedback Thanks Paul F. Lambert, Ph.D. Professor of Oncology McArdle Laboratory for Cancer Research University of Wisconsin School of Medicine and Public Health 1400 University Ave. Madison WI 54706 USA tel: 608-262-8533 fax: 608-262-2824 email: plambert@wisc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From david.w.anderson <@t> Vanderbilt.Edu Wed Feb 3 12:10:24 2010 From: david.w.anderson <@t> Vanderbilt.Edu (Anderson, David W) Date: Wed Feb 3 12:10:30 2010 Subject: [Histonet] GI Biopsies Message-ID: <47299A40AEED044DA3255843A948CBCD03251A6F5A@ITS-HCWNEM02.ds.Vanderbilt.edu> We are having an issue with our GI Biopsy H&E Stains. On our GI Biopsies, we currently cut eight slides, staining levels 2, 5 and 8 with H&E. The pathologist are noticing that the level two slide looks great but then levels 5 and 8 will have what they call "Blue Blobs" on the tissue sections. They tend to look like formalin salt crystals. Does anyone else experience this? Do any of you have a resolution to this problem? Thank you so much for your help with this matter. David Anderson,HT(ASCP) From Sharon.Davis-Devine <@t> carle.com Wed Feb 3 12:13:09 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Feb 3 12:13:55 2010 Subject: [Histonet] P63 Message-ID: <50003EC02E2CEA4583BEB3CD08EAC1E090DDEE@EXCHANGEBE2.carle.com> Do any of you Ventana users have a P63 that is not an RUO? If so, where do you get it from? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From mpence <@t> grhs.net Wed Feb 3 12:20:52 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Feb 3 12:21:01 2010 Subject: [Histonet] P63 In-Reply-To: <50003EC02E2CEA4583BEB3CD08EAC1E090DDEE@EXCHANGEBE2.carle.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D37@is-e2k3.grhs.net> >From Cell Marque -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, February 03, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P63 Do any of you Ventana users have a P63 that is not an RUO? If so, where do you get it from? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SDrew <@t> uwhealth.org Wed Feb 3 12:21:56 2010 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Wed Feb 3 12:22:16 2010 Subject: [Histonet] P63 In-Reply-To: <50003EC02E2CEA4583BEB3CD08EAC1E090DDEE@EXCHANGEBE2.carle.com> Message-ID: <738A7878143FF74BB77436E255743C1A0100049E@UWHC-MAIL03.uwhis.hosp.wisc.edu> We use an IVD p63 (cat# CM163) from BioCare Medical. Contact me if you'd like further info. Sally Ann Drew, MT(ASCP) IHC/ISH Clinical & Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison, WI 53792 (608)265-6596 Fax:(608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, February 03, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P63 Do any of you Ventana users have a P63 that is not an RUO? If so, where do you get it from? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Wed Feb 3 12:49:28 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Feb 3 12:49:39 2010 Subject: [Histonet] RE: GI Biopsies In-Reply-To: <47299A40AEED044DA3255843A948CBCD03251A6F5A@ITS-HCWNEM02.ds.Vanderbilt.edu> References: <47299A40AEED044DA3255843A948CBCD03251A6F5A@ITS-HCWNEM02.ds.Vanderbilt.edu> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE46FADC06@EXCHMBC2.ad.ah.local> What kind of stainer do you have? Jan, Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anderson, David W Sent: Wednesday, February 03, 2010 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI Biopsies We are having an issue with our GI Biopsy H&E Stains. On our GI Biopsies, we currently cut eight slides, staining levels 2, 5 and 8 with H&E. The pathologist are noticing that the level two slide looks great but then levels 5 and 8 will have what they call "Blue Blobs" on the tissue sections. They tend to look like formalin salt crystals. Does anyone else experience this? Do any of you have a resolution to this problem? Thank you so much for your help with this matter. David Anderson,HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Joyce.Cline <@t> wchsys.org Wed Feb 3 13:01:50 2010 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Wed Feb 3 13:07:01 2010 Subject: [Histonet] Eosin leaching out of sections In-Reply-To: References: Message-ID: Humidity has played a large part in our slides leaching Eosin. We have a dehumidifier in our staining room and we also use H2Blue Beads in our Xylene substitute. The beads absorb water from the substitute and help prevent leaching of the stain. The beads are ordered from Amercian Mastertech. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From trathborne <@t> somerset-healthcare.com Wed Feb 3 13:11:00 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Feb 3 13:11:09 2010 Subject: [Histonet] Eosin leaching out of sections In-Reply-To: Message-ID: Where do you get the beads from? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joyce Cline Sent: Wednesday, February 03, 2010 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Eosin leaching out of sections Humidity has played a large part in our slides leaching Eosin. We have a dehumidifier in our staining room and we also use H2Blue Beads in our Xylene substitute. The beads absorb water from the substitute and help prevent leaching of the stain. The beads are ordered from Amercian Mastertech. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Sharon.Davis-Devine <@t> carle.com Wed Feb 3 13:28:07 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Feb 3 13:28:50 2010 Subject: [Histonet] P63 RUO Message-ID: <50003EC02E2CEA4583BEB3CD08EAC1E090DDF1@EXCHANGEBE2.carle.com> Another question for the group of experts. Is it true that you cannot bill for antibodies that are classified as a RUO for Ventana? Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Feb 3 14:03:50 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Feb 3 14:04:46 2010 Subject: [Histonet] RE: P63 RUO In-Reply-To: <50003EC02E2CEA4583BEB3CD08EAC1E090DDF1@EXCHANGEBE2.carle.com> References: <50003EC02E2CEA4583BEB3CD08EAC1E090DDF1@EXCHANGEBE2.carle.com> Message-ID: It doesn't matter what machine you run them on or if you do them by hand. You cannot bill for anything that is RUO. ASR and IVD's muct be properly validated before you bill for them as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine [Sharon.Davis-Devine@carle.com] Sent: Wednesday, February 03, 2010 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P63 RUO Another question for the group of experts. Is it true that you cannot bill for antibodies that are classified as a RUO for Ventana? Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Wed Feb 3 15:20:03 2010 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Feb 3 15:20:24 2010 Subject: [Histonet] Microarray info thanks! Message-ID: <8CC732DC90E018F-1D5C-2A59@webmail-m095.sysops.aol.com> Thank you everyone for all the information about microarray equipment. As usual on the Histonet, everyone was quick with the information, generous with their time, and informative. Michael Titford USA Pathology Mobile AL USA From pathmaster <@t> yahoo.com Wed Feb 3 16:04:20 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Wed Feb 3 16:04:23 2010 Subject: [Histonet] Drying ovens, eosin fading, microtome PM, eyeballs Message-ID: <333638.6266.qm@web111109.mail.gq1.yahoo.com> Our routine sections,? picked up on regular slides with no adhesive in the water bath, ?dry at 65C for 15 minutes in the oven on our Leica stainer XL, we never lose sections. Sections for IHC and specials go on Plus slides and dry in an oven also at 65C for at least 1 hour.? Then they get the same ?Leica XL oven drying cycle before deceration. ? Fading eosin usually means incomplete dehydration, trace water in the xylene etc. ? Belair does all our PM's, microtomes, cryostats, they've kept our 30 year old VIP running despite the fact that Sakura no longer supports the oldest models (like ours). ? Eyeballs should not be opened until they have been fixed for at least two days intact in 10% buffered formalin. Then, using a brand new? blade, slice off the two callottes, proceeding from cornea posteriorly to the optic nerve, ?from a central section which includes the optic nerve and the pupil. Some retinal separation is inevitable, this technique will minimize it. ? Jeffrey Silverman, HT HTL QIHC Pathologists' Assistant Southside Hospital NSLIJHS Bay Shore, New York USA ? ? From MSHERWOOD <@t> PARTNERS.ORG Wed Feb 3 16:10:38 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Feb 3 16:10:42 2010 Subject: [Histonet] TRAP assay - acid phosphatase Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23FDC@PHSXMB30.partners.org> Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on mouse tibia for IHC. If so, 1) can you do it on paraffin and/or frozen sections? 2) if you do it on frozens, do you decalcify first? 3) what type of blade do you use for sectioning on the cryostat? 3) what procedure do you follow after sectioning: do you fix slides (i.e. acetone, etc.) Thank you. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From ayreskyle <@t> yahoo.com Wed Feb 3 16:47:47 2010 From: ayreskyle <@t> yahoo.com (kyle ayres) Date: Wed Feb 3 16:47:52 2010 Subject: [Histonet] slide drying Message-ID: <47296.74380.qm@web31907.mail.mud.yahoo.com> Place the slides in every other slot of basket and dry for 15-20 min at 69 celcius, let cool in front of fan for 1-2 min. You are able to combine baskets at this step. ? Kyle HT BS Nacogdoches Memorial ? ? ? Our lab just received a slide drying oven, and we are trying to figure out what's a good temperature for the slides to be heated and for how long. We are mainly doing H&E slides. The way we are currently drying them is under a small fan, then heat them up, and back to the fan. Then finally heating them up and off to the stainer. Just putting them in the slide drying oven for 10 min at 80C melts the paraffin, but leaves some water on the bottom of some sections. Any suggestions to get the slides dry asap ?? Also is the oven much help to your labs? Thanks ! Scott Hendricksen HT(ASCP) From liz <@t> premierlab.com Wed Feb 3 16:48:31 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Feb 3 16:49:21 2010 Subject: [Histonet] TRAP assay - acid phosphatase References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23FDC@PHSXMB30.partners.org> Message-ID: We have used it on FFPE EDTA decaled mouse bone, with a little bit of fiddling with the protocol it works pretty good Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sherwood, Margaret Sent: Wed 2/3/2010 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TRAP assay - acid phosphatase Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on mouse tibia for IHC. If so, 1) can you do it on paraffin and/or frozen sections? 2) if you do it on frozens, do you decalcify first? 3) what type of blade do you use for sectioning on the cryostat? 3) what procedure do you follow after sectioning: do you fix slides (i.e. acetone, etc.) Thank you. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sondra.Delaney <@t> Integris-Health.com Wed Feb 3 17:13:43 2010 From: Sondra.Delaney <@t> Integris-Health.com (Delaney, Sondra L) Date: Wed Feb 3 17:13:49 2010 Subject: [Histonet] FW: FFPE Microwave processed biopsies Message-ID: <70F29E1F984F6D45A07F071AFAAFC6D308C1AFCA63@INTEGRISMAIL.corp.integris-health.com> -----Original Message----- From: Chad Miller [mailto:cmiller@labmd.org] Sent: Wednesday, February 03, 2010 4:46 PM To: Delaney, Sondra L Cc: Chad Miller Subject: FFPE Microwave processed biopsies Microtomy Issue: Our lab has recently switched from using GTF (Glycol Tissue Fixative) as a primary fixative to10%NBF for prostate biopsies. I am experiencing issues with wrinkles, to varying degrees, along the length of the biopsies especially around the lumens of glandular structures. Sections are taken at 3 micrometers with zero visible compression/wrinkling/washboarding etc... of the tissue. When the ribbon is placed on the water bath (47-50 C) the tissue seems to contract and wrinkling appears although, only in the tissue....similar to the coils of a compressed slinky. I have adjusted the water bath temp. increasingly higher until my wax can no longer stand the temp and dissipates around the tissue. This has not cured the wrinkling problem but has shown some improvement. Prostate biopsies that have been fixed in GTF and processed identically to the formalin fixed biopsies are simply perfect in every way. Specimens are embedded using Polyfin (TBS, M.P. 55C) and kept cool for sectioning on ice trays. Sections are collected on charged Histobond slides. Wrinkles appear whether processing solutions are brand new or have been used for previous runs. If anyone has any suggestions ideas or experiences I would sincerely appreciate them. I have included processing times below. Specimen Type: Prostate Needle Core Biopsy Processor: Milestone Histos 5 (Microwave) Specimen Fixative: 10%NBF Specimen Prep: Aligned in linear fashion and sandwiched between sponges to maintain orientation. Max Block Volume: 110 blocks/run Offline Prep: 1. 70% ETOH Rinse (Manual @ 5minutes)--(Changed every 450 blks) 2. 95% ETOH Rinse (Manual @ 5minutes)--(Changed every 450 blks) 3. 100% ETOH Rinse (Manual @ 5minutes)--(Changed every 450 blks) Histos 5 Processor: (0-55blk processing protocol) 1. 100% ETOH (15 minutes @ 65 C)--(Changed every 220 blks) 2. Isopropyl (16 minutes @ 68 C)--(Changed every 220 blks) 3. Vacuum Step (2 minutes ambient) 4. Paraffin (Paraplast M.P.56 C) (23 minutes @ 68 C with Vacuum)-(Changed every 450 blks) This e-mail may contain identifiable health information that is subject to protection under state and federal law. This information is intended to be for the use of the individual named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited and may be punishable by law. If you have received this electronic transmission in error, please notify us immediately by electronic mail (reply). From collette2 <@t> mail.llnl.gov Wed Feb 3 18:11:13 2010 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Wed Feb 3 18:11:02 2010 Subject: [Histonet] autofluorescence experiment Message-ID: Hello, All, I am working on doing some IF stains with bone samples (lucky me!). I am having a difficult time sometimes to assess the antibody since the autofluorescence gets in the way. I am using undecalcified, FFPE sections (late embryo and neonate mouse bones). Without treatment, I see autofluorescence everywhere, but most frustrating is the red blood cells and the mineralized matrix. It took me a while to get samples that are properly fixed, section well, and stay on the slides, so I am not particularly jazzed about messing with the fixation protocol. Thus, I conducted an experiment today of several published and voodoo methods to reduce autofluorescence with samples that did NOT undergo the IF protocol : no treatment photobleaching, fluorescent light box, up to 2 weeks photobleaching, UV crosslinking light, 2 inches from source, 24h 10mM copper sulfate in 50mM ammonium acetate, pH 5.0 0.25% (v/v) NH3 in 70% ethanol (in water) 0.25% (v/v) NH4OH in 70% ethanol (in water) (since it was unclear from the published reference what the source of ammonia was, and I have made this mistake before on some other thing) 0.3% (w/v) Sudan Black in 70% ethanol (in water) I found that the most effective treatment in my hands is Sudan Black for cell-based autofluorescence, but it did not seem to impact the autofluorescence from the mineralized matrix at all, while copper sulfate had significant impact on the autofluorescence in mineralized bone, but did not quench cell-based autofluorescence as well as the Sudan Black (it had an even but incomplete impact over the entire section). I have tried Sudan Black on my slides before, and have found that it did not seem to interfere significantly with the antibody signal. I modified the Sudan Black protocol to eliminate the "goopies" and "chunkies" resulting on my slides from previous attempts, and am happy with the results- a light, even stain. My question to all you chemistry folks: Is there some reason why copper sulfate treatment followed by washing and subsequent Sudan Black treatment (and more washing) cannot or should not be used? I tried them together on my unstained slides, they look pretty darn fabulous. Just striving for clean data and beautiful pictures. Thanks again for all your help, Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley From soofias2 <@t> yahoo.com Wed Feb 3 19:42:18 2010 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Wed Feb 3 19:42:24 2010 Subject: [Histonet] Derm Lab In-Reply-To: <6c3840890912300640p70132627w9befa4fcca15117f@mail.gmail.com> Message-ID: <692051.1111.qm@web112503.mail.gq1.yahoo.com> Raj, If you answer Eric's email I may help you? too, because I set up a highly complex testing lab at dermatology starting from scratch with an empty room when they were starting the remodeling the room. The room?may ?have ?already been equipped with ventilation, plumbing and electrical but they still had to put outlets and design the working are and bench surface. Soofia --- On Wed, 12/30/09, Eric Sulkosky wrote: From: Eric Sulkosky Subject: [Histonet] Derm Lab To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 30, 2009, 8:40 AM RAJ, What tips are you looking for? Are you starting from scratch with an empty room or has the room already been equipped with ventilation, plumbing and electrical? Eric _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From soofias2 <@t> yahoo.com Thu Feb 4 00:11:03 2010 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Thu Feb 4 00:11:10 2010 Subject: [Histonet] Frozen Sections Slides per Day Question Message-ID: <552462.75622.qm@web112516.mail.gq1.yahoo.com> Hi dear histology experts, I will greatly appreciate if somebody can let me know the estimated number of slides (with 3 sections per slide) per day on average a lab technician can cut (of frozen tissue). ? I am a lab technician?( not a histotech)?and?work alone in?a highly complex testing specialzed low volume dermatology lab.My job description?includes 30 % of immunohistochemistry related dutites. ?I cut skin frozen sections and do immunohistochemistry? manually for several years?. My routiene panel?consists of?12?antibodies for ?T-cells??surface markers. Ocassionally??I?add another panel of 8 antibodies for B-Cells. I am very slow in cutting sections and strugle a lot to get good sections. If I spend entire day (?8 hours)?just ?cutting? 3-4 section on each slide with slow speed. What? number of?slides should be considered as efficent cutting? What number of blocks (12 slides for each block with 3-4 sections) should I finish in one day? ? Help me please if you can. Thanks. Soofia ? From lloyd.3 <@t> osu.edu Thu Feb 4 04:48:38 2010 From: lloyd.3 <@t> osu.edu (Mary Lloyd) Date: Thu Feb 4 04:48:42 2010 Subject: [Histonet] mailers Message-ID: <04A71EFE05FE5F4DAA05D0500FD0597002A014F1@Distal.dentnet.dent.ohio-state.edu> We have been using a round double mailer with a metal insert from Fisher Scientific. The size for the outside cardboard mailer is 2 3/8" by 5 3/4 inch. Fisher has discontinued this mailer. Does anyone know a reasonable company that would carry these mailers. I found a company that has a similar product that is larger, but our cost for mailing will increase to mail the containers. I would appreciate any help. Thanks From KPercival <@t> wyeth.com Thu Feb 4 08:15:19 2010 From: KPercival <@t> wyeth.com (Percival Karen) Date: Thu Feb 4 08:15:36 2010 Subject: [Histonet] Tape/Film Coverslips vs. Glass In-Reply-To: <000001caa4dc$25705680$70510380$@com> References: <000001caa4dc$25705680$70510380$@com> Message-ID: <4B6A902702000011001F84DC@gv01a67m.gv.us.pri.wyeth.com> I've used both over the years, and I love the tape coverslippers. The slides dry very quickly because there is no mounting media step. The tape sticks to the slide via a chemical reaction between the xylene and the tape. One thing to remember with tape, you cannot use recycled xylene as your last xylene step before coverslipping. If I remember correctly, you cannot use xylene substitutes either. It's been a while, so that may have changed. The tape coverslips are easily removed with acetone. The pathologists loved it because they received their slides immediately. We didn't have to allow drying time before handing them in. I didn't find any tissue fading over time, either. Just my opinion. Good luck. >>> "Cynthia Pyse" 2/3/2010 9:21 AM >>> Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy.Crumpton@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?&nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgrow <@t> bmnet.com Thu Feb 4 09:05:30 2010 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Thu Feb 4 09:05:34 2010 Subject: [Histonet] Re: Eosin leaching out of sections In-Reply-To: Message-ID: Hi Scott, Your discription of eosin leaching out after a few days is classic for the possibility of bleach contamination on your staining containers. There are multiple possibilities of other causes, but this could be a simple cause, easily solved. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) _______________________________________________ From MSHERWOOD <@t> PARTNERS.ORG Thu Feb 4 09:19:43 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Feb 4 09:19:48 2010 Subject: [Histonet] TRAP Assay and IHC Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23FDE@PHSXMB30.partners.org> I want to thank everyone who responded to my inquiry re: TRAP Assay. I don't think I made my request that clear re: IHC. We want to do both TRAP Assay and IHC (separately, of course) on mouse tibia. We would like to use frozen sections of mouse tibia for IHC. Our initial try to cut tibia on the cryostat has resulted in less than optimal sections. It appears that the tissue is lifting out of the section. In re: IHC on frozens - 1) do you treat the tibia before embedding in OCT? 2) what knife do you cut with on the cryostat? 3) do you fix slides with cold acetone before the IHC procedure (we ususally do)? Thanks again! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rfields <@t> gidocs.net Thu Feb 4 09:18:54 2010 From: rfields <@t> gidocs.net (Rosa Fields) Date: Thu Feb 4 09:20:30 2010 Subject: [Histonet] Tape/Film Coverslips vs. Glass References: <000001caa4dc$25705680$70510380$@com> <4B6A902702000011001F84DC@gv01a67m.gv.us.pri.wyeth.com> Message-ID: <07732CE52EC3174AB891DE1C62DB4D8FC7B6A3@GIEXCHANGE.gidocs.net> The tape cover slippers are quicker, easier to manage the slides, but the cover slips pop off over time; the glass cover slippers are a bit temperamental; the slides take longer to dry, and the quality is better, and the cover slip stays on. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Percival Karen Sent: Thursday, February 04, 2010 8:15 AM To: histonet@lists.utsouthwestern.edu; Cathy.Crumpton@tuality.org; Pyse Cynthia Subject: RE: [Histonet] Tape/Film Coverslips vs. Glass I've used both over the years, and I love the tape coverslippers. The slides dry very quickly because there is no mounting media step. The tape sticks to the slide via a chemical reaction between the xylene and the tape. One thing to remember with tape, you cannot use recycled xylene as your last xylene step before coverslipping. If I remember correctly, you cannot use xylene substitutes either. It's been a while, so that may have changed. The tape coverslips are easily removed with acetone. The pathologists loved it because they received their slides immediately. We didn't have to allow drying time before handing them in. I didn't find any tissue fading over time, either. Just my opinion. Good luck. >>> "Cynthia Pyse" 2/3/2010 9:21 AM >>> Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy.Crumpton@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?&nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From judith_pardue <@t> memorial.org Thu Feb 4 09:38:38 2010 From: judith_pardue <@t> memorial.org (Pardue, Judith) Date: Thu Feb 4 09:38:54 2010 Subject: [Histonet] waterbaths Message-ID: Does anyone know if a waterbath temp is set to low ( 37C degrees) if the staining will be effected by this. Judith Pardue Memorial Healthcare System Chattanooga,Tn. judith_pardue@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From MadaryJ <@t> MedImmune.com Thu Feb 4 09:45:39 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Thu Feb 4 09:45:52 2010 Subject: [Histonet] trap fixation and decal my 2 cents In-Reply-To: References: Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1301268C9F@MD1EV002.medimmune.com> The one thing I do recall was to cold decal in EDTA after cold fixation. I think it helped avoid some inconsistent staining, at least in our hands. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, February 04, 2010 10:27 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. GI Biopsies (Anderson, David W) 2. P63 (Sharon.Davis-Devine) 3. RE: P63 (Mike Pence) 4. RE: P63 (Drew Sally A) 5. RE: GI Biopsies (Mahoney,Janice A) 6. RE: Eosin leaching out of sections (Joyce Cline) 7. RE: Eosin leaching out of sections (Rathborne, Toni) 8. P63 RUO (Sharon.Davis-Devine) 9. RE: P63 RUO (McMahon, Loralee A) 10. Microarray info thanks! (mtitford@aol.com) 11. Drying ovens, eosin fading, microtome PM, eyeballs (Jeffrey Silverman) 12. Re: TRAP assay - acid phosphatase (Sherwood, Margaret ) 13. slide drying (kyle ayres) 14. RE: TRAP assay - acid phosphatase (Liz Chlipala) 15. FW: FFPE Microwave processed biopsies (Delaney, Sondra L) 16. autofluorescence experiment (Nicole Collette) 17. Re: Derm Lab (soofia siddiqui) 18. Frozen Sections Slides per Day Question (soofia siddiqui) 19. mailers (Mary Lloyd) 20. RE: Tape/Film Coverslips vs. Glass (Percival Karen) 21. Re: Eosin leaching out of sections (rgrow@bmnet.com) 22. Re: TRAP Assay and IHC (Sherwood, Margaret ) 23. RE: Tape/Film Coverslips vs. Glass (Rosa Fields) ---------------------------------------------------------------------- Message: 1 Date: Wed, 3 Feb 2010 12:10:24 -0600 From: "Anderson, David W" Subject: [Histonet] GI Biopsies To: "histonet@lists.utsouthwestern.edu" Message-ID: <47299A40AEED044DA3255843A948CBCD03251A6F5A@ITS-HCWNEM02.ds.Vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" We are having an issue with our GI Biopsy H&E Stains. On our GI Biopsies, we currently cut eight slides, staining levels 2, 5 and 8 with H&E. The pathologist are noticing that the level two slide looks great but then levels 5 and 8 will have what they call "Blue Blobs" on the tissue sections. They tend to look like formalin salt crystals. Does anyone else experience this? Do any of you have a resolution to this problem? Thank you so much for your help with this matter. David Anderson,HT(ASCP) ------------------------------ Message: 2 Date: Wed, 3 Feb 2010 12:13:09 -0600 From: "Sharon.Davis-Devine" Subject: [Histonet] P63 To: Message-ID: <50003EC02E2CEA4583BEB3CD08EAC1E090DDEE@EXCHANGEBE2.carle.com> Content-Type: text/plain; charset="us-ascii" Do any of you Ventana users have a P63 that is not an RUO? If so, where do you get it from? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com ------------------------------ Message: 3 Date: Wed, 3 Feb 2010 12:20:52 -0600 From: "Mike Pence" Subject: RE: [Histonet] P63 To: "Sharon.Davis-Devine" , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D37@is-e2k3.grhs.net> Content-Type: text/plain; charset="us-ascii" >From Cell Marque -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, February 03, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P63 Do any of you Ventana users have a P63 that is not an RUO? If so, where do you get it from? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 3 Feb 2010 12:21:56 -0600 From: "Drew Sally A" Subject: RE: [Histonet] P63 To: "Histonet" Message-ID: <738A7878143FF74BB77436E255743C1A0100049E@UWHC-MAIL03.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="US-ASCII" We use an IVD p63 (cat# CM163) from BioCare Medical. Contact me if you'd like further info. Sally Ann Drew, MT(ASCP) IHC/ISH Clinical & Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison, WI 53792 (608)265-6596 Fax:(608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, February 03, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P63 Do any of you Ventana users have a P63 that is not an RUO? If so, where do you get it from? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 3 Feb 2010 12:49:28 -0600 From: "Mahoney,Janice A" Subject: [Histonet] RE: GI Biopsies To: "'Anderson, David W'" , "histonet@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE46FADC06@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" What kind of stainer do you have? Jan, Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anderson, David W Sent: Wednesday, February 03, 2010 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI Biopsies We are having an issue with our GI Biopsy H&E Stains. On our GI Biopsies, we currently cut eight slides, staining levels 2, 5 and 8 with H&E. The pathologist are noticing that the level two slide looks great but then levels 5 and 8 will have what they call "Blue Blobs" on the tissue sections. They tend to look like formalin salt crystals. Does anyone else experience this? Do any of you have a resolution to this problem? Thank you so much for your help with this matter. David Anderson,HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 6 Date: Wed, 3 Feb 2010 14:01:50 -0500 From: Joyce Cline Subject: RE: [Histonet] Eosin leaching out of sections To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Humidity has played a large part in our slides leaching Eosin. We have a dehumidifier in our staining room and we also use H2Blue Beads in our Xylene substitute. The beads absorb water from the substitute and help prevent leaching of the stain. The beads are ordered from Amercian Mastertech. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 7 Date: Wed, 3 Feb 2010 14:11:00 -0500 From: "Rathborne, Toni" Subject: RE: [Histonet] Eosin leaching out of sections To: "Joyce Cline" , Message-ID: Content-Type: text/plain; charset="utf-8" Where do you get the beads from? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joyce Cline Sent: Wednesday, February 03, 2010 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Eosin leaching out of sections Humidity has played a large part in our slides leaching Eosin. We have a dehumidifier in our staining room and we also use H2Blue Beads in our Xylene substitute. The beads absorb water from the substitute and help prevent leaching of the stain. The beads are ordered from Amercian Mastertech. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 8 Date: Wed, 3 Feb 2010 13:28:07 -0600 From: "Sharon.Davis-Devine" Subject: [Histonet] P63 RUO To: Message-ID: <50003EC02E2CEA4583BEB3CD08EAC1E090DDF1@EXCHANGEBE2.carle.com> Content-Type: text/plain; charset="us-ascii" Another question for the group of experts. Is it true that you cannot bill for antibodies that are classified as a RUO for Ventana? Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com ------------------------------ Message: 9 Date: Wed, 3 Feb 2010 15:03:50 -0500 From: "McMahon, Loralee A" Subject: [Histonet] RE: P63 RUO To: "Sharon.Davis-Devine" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" It doesn't matter what machine you run them on or if you do them by hand. You cannot bill for anything that is RUO. ASR and IVD's muct be properly validated before you bill for them as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine [Sharon.Davis-Devine@carle.com] Sent: Wednesday, February 03, 2010 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P63 RUO Another question for the group of experts. Is it true that you cannot bill for antibodies that are classified as a RUO for Ventana? Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 03 Feb 2010 16:20:03 -0500 From: mtitford@aol.com Subject: [Histonet] Microarray info thanks! To: Histonet@pathology.swmed.edu Message-ID: <8CC732DC90E018F-1D5C-2A59@webmail-m095.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Thank you everyone for all the information about microarray equipment. As usual on the Histonet, everyone was quick with the information, generous with their time, and informative. Michael Titford USA Pathology Mobile AL USA ------------------------------ Message: 11 Date: Wed, 3 Feb 2010 14:04:20 -0800 (PST) From: Jeffrey Silverman Subject: [Histonet] Drying ovens, eosin fading, microtome PM, eyeballs To: Histonet@lists.utsouthwestern.edu Message-ID: <333638.6266.qm@web111109.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Our routine sections,? picked up on regular slides with no adhesive in the water bath, ?dry at 65C for 15 minutes in the oven on our Leica stainer XL, we never lose sections. Sections for IHC and specials go on Plus slides and dry in an oven also at 65C for at least 1 hour.? Then they get the same ?Leica XL oven drying cycle before deceration. ? Fading eosin usually means incomplete dehydration, trace water in the xylene etc. ? Belair does all our PM's, microtomes, cryostats, they've kept our 30 year old VIP running despite the fact that Sakura no longer supports the oldest models (like ours). ? Eyeballs should not be opened until they have been fixed for at least two days intact in 10% buffered formalin. Then, using a brand new? blade, slice off the two callottes, proceeding from cornea posteriorly to the optic nerve, ?from a central section which includes the optic nerve and the pupil. Some retinal separation is inevitable, this technique will minimize it. ? Jeffrey Silverman, HT HTL QIHC Pathologists' Assistant Southside Hospital NSLIJHS Bay Shore, New York USA ? ? ------------------------------ Message: 12 Date: Wed, 3 Feb 2010 17:10:38 -0500 From: "Sherwood, Margaret " Subject: Re: [Histonet] TRAP assay - acid phosphatase To: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23FDC@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on mouse tibia for IHC. If so, 1) can you do it on paraffin and/or frozen sections? 2) if you do it on frozens, do you decalcify first? 3) what type of blade do you use for sectioning on the cryostat? 3) what procedure do you follow after sectioning: do you fix slides (i.e. acetone, etc.) Thank you. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 13 Date: Wed, 3 Feb 2010 14:47:47 -0800 (PST) From: kyle ayres Subject: [Histonet] slide drying To: Histonet@lists.utsouthwestern.edu Message-ID: <47296.74380.qm@web31907.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Place the slides in every other slot of basket and dry for 15-20 min at 69 celcius, let cool in front of fan for 1-2 min. You are able to combine baskets at this step. ? Kyle HT BS Nacogdoches Memorial ? ? ? Our lab just received a slide drying oven, and we are trying to figure out what's a good temperature for the slides to be heated and for how long. We are mainly doing H&E slides. The way we are currently drying them is under a small fan, then heat them up, and back to the fan. Then finally heating them up and off to the stainer. Just putting them in the slide drying oven for 10 min at 80C melts the paraffin, but leaves some water on the bottom of some sections. Any suggestions to get the slides dry asap ?? Also is the oven much help to your labs? Thanks ! Scott Hendricksen HT(ASCP) ------------------------------ Message: 14 Date: Wed, 3 Feb 2010 15:48:31 -0700 From: "Liz Chlipala" Subject: RE: [Histonet] TRAP assay - acid phosphatase To: "Sherwood, Margaret " , Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have used it on FFPE EDTA decaled mouse bone, with a little bit of fiddling with the protocol it works pretty good Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sherwood, Margaret Sent: Wed 2/3/2010 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TRAP assay - acid phosphatase Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on mouse tibia for IHC. If so, 1) can you do it on paraffin and/or frozen sections? 2) if you do it on frozens, do you decalcify first? 3) what type of blade do you use for sectioning on the cryostat? 3) what procedure do you follow after sectioning: do you fix slides (i.e. acetone, etc.) Thank you. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 3 Feb 2010 17:13:43 -0600 From: "Delaney, Sondra L" Subject: [Histonet] FW: FFPE Microwave processed biopsies To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <70F29E1F984F6D45A07F071AFAAFC6D308C1AFCA63@INTEGRISMAIL.corp.integris-health.com> Content-Type: text/plain; charset="us-ascii" -----Original Message----- From: Chad Miller [mailto:cmiller@labmd.org] Sent: Wednesday, February 03, 2010 4:46 PM To: Delaney, Sondra L Cc: Chad Miller Subject: FFPE Microwave processed biopsies Microtomy Issue: Our lab has recently switched from using GTF (Glycol Tissue Fixative) as a primary fixative to10%NBF for prostate biopsies. I am experiencing issues with wrinkles, to varying degrees, along the length of the biopsies especially around the lumens of glandular structures. Sections are taken at 3 micrometers with zero visible compression/wrinkling/washboarding etc... of the tissue. When the ribbon is placed on the water bath (47-50 C) the tissue seems to contract and wrinkling appears although, only in the tissue....similar to the coils of a compressed slinky. I have adjusted the water bath temp. increasingly higher until my wax can no longer stand the temp and dissipates around the tissue. This has not cured the wrinkling problem but has shown some improvement. Prostate biopsies that have been fixed in GTF and processed identically to the formalin fixed biopsies are simply perfect in every way. Specimens are embedded using Polyfin (TBS, M.P. 55C) and kept cool for sectioning on ice trays. Sections are collected on charged Histobond slides. Wrinkles appear whether processing solutions are brand new or have been used for previous runs. If anyone has any suggestions ideas or experiences I would sincerely appreciate them. I have included processing times below. Specimen Type: Prostate Needle Core Biopsy Processor: Milestone Histos 5 (Microwave) Specimen Fixative: 10%NBF Specimen Prep: Aligned in linear fashion and sandwiched between sponges to maintain orientation. Max Block Volume: 110 blocks/run Offline Prep: 1. 70% ETOH Rinse (Manual @ 5minutes)--(Changed every 450 blks) 2. 95% ETOH Rinse (Manual @ 5minutes)--(Changed every 450 blks) 3. 100% ETOH Rinse (Manual @ 5minutes)--(Changed every 450 blks) Histos 5 Processor: (0-55blk processing protocol) 1. 100% ETOH (15 minutes @ 65 C)--(Changed every 220 blks) 2. Isopropyl (16 minutes @ 68 C)--(Changed every 220 blks) 3. Vacuum Step (2 minutes ambient) 4. Paraffin (Paraplast M.P.56 C) (23 minutes @ 68 C with Vacuum)-(Changed every 450 blks) This e-mail may contain identifiable health information that is subject to protection under state and federal law. This information is intended to be for the use of the individual named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited and may be punishable by law. If you have received this electronic transmission in error, please notify us immediately by electronic mail (reply). ------------------------------ Message: 16 Date: Wed, 3 Feb 2010 16:11:13 -0800 From: Nicole Collette Subject: [Histonet] autofluorescence experiment To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Hello, All, I am working on doing some IF stains with bone samples (lucky me!). I am having a difficult time sometimes to assess the antibody since the autofluorescence gets in the way. I am using undecalcified, FFPE sections (late embryo and neonate mouse bones). Without treatment, I see autofluorescence everywhere, but most frustrating is the red blood cells and the mineralized matrix. It took me a while to get samples that are properly fixed, section well, and stay on the slides, so I am not particularly jazzed about messing with the fixation protocol. Thus, I conducted an experiment today of several published and voodoo methods to reduce autofluorescence with samples that did NOT undergo the IF protocol : no treatment photobleaching, fluorescent light box, up to 2 weeks photobleaching, UV crosslinking light, 2 inches from source, 24h 10mM copper sulfate in 50mM ammonium acetate, pH 5.0 0.25% (v/v) NH3 in 70% ethanol (in water) 0.25% (v/v) NH4OH in 70% ethanol (in water) (since it was unclear from the published reference what the source of ammonia was, and I have made this mistake before on some other thing) 0.3% (w/v) Sudan Black in 70% ethanol (in water) I found that the most effective treatment in my hands is Sudan Black for cell-based autofluorescence, but it did not seem to impact the autofluorescence from the mineralized matrix at all, while copper sulfate had significant impact on the autofluorescence in mineralized bone, but did not quench cell-based autofluorescence as well as the Sudan Black (it had an even but incomplete impact over the entire section). I have tried Sudan Black on my slides before, and have found that it did not seem to interfere significantly with the antibody signal. I modified the Sudan Black protocol to eliminate the "goopies" and "chunkies" resulting on my slides from previous attempts, and am happy with the results- a light, even stain. My question to all you chemistry folks: Is there some reason why copper sulfate treatment followed by washing and subsequent Sudan Black treatment (and more washing) cannot or should not be used? I tried them together on my unstained slides, they look pretty darn fabulous. Just striving for clean data and beautiful pictures. Thanks again for all your help, Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley ------------------------------ Message: 17 Date: Wed, 3 Feb 2010 17:42:18 -0800 (PST) From: soofia siddiqui Subject: Re: [Histonet] Derm Lab To: histonet@lists.utsouthwestern.edu, Eric Sulkosky Message-ID: <692051.1111.qm@web112503.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Raj, If you answer Eric's email I may help you? too, because I set up a highly complex testing lab at dermatology starting from scratch with an empty room when they were starting the remodeling the room. The room?may ?have ?already been equipped with ventilation, plumbing and electrical but they still had to put outlets and design the working are and bench surface. Soofia --- On Wed, 12/30/09, Eric Sulkosky wrote: From: Eric Sulkosky Subject: [Histonet] Derm Lab To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 30, 2009, 8:40 AM RAJ, What tips are you looking for? Are you starting from scratch with an empty room or has the room already been equipped with ventilation, plumbing and electrical? Eric _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 3 Feb 2010 22:11:03 -0800 (PST) From: soofia siddiqui Subject: [Histonet] Frozen Sections Slides per Day Question To: Histonet@lists.utsouthwestern.edu Message-ID: <552462.75622.qm@web112516.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi dear histology experts, I will greatly appreciate if somebody can let me know the estimated number of slides (with 3 sections per slide) per day on average a lab technician can cut (of frozen tissue). ? I am a lab technician?( not a histotech)?and?work alone in?a highly complex testing specialzed low volume dermatology lab.My job description?includes 30 % of immunohistochemistry related dutites. ?I cut skin frozen sections and do immunohistochemistry? manually for several years?. My routiene panel?consists of?12?antibodies for ?T-cells??surface markers. Ocassionally??I?add another panel of 8 antibodies for B-Cells. I am very slow in cutting sections and strugle a lot to get good sections. If I spend entire day (?8 hours)?just ?cutting? 3-4 section on each slide with slow speed. What? number of?slides should be considered as efficent cutting? What number of blocks (12 slides for each block with 3-4 sections) should I finish in one day? ? Help me please if you can. Thanks. Soofia ? ------------------------------ Message: 19 Date: Thu, 4 Feb 2010 05:48:38 -0500 From: "Mary Lloyd" Subject: [Histonet] mailers To: Message-ID: <04A71EFE05FE5F4DAA05D0500FD0597002A014F1@Distal.dentnet.dent.ohio-state.edu> Content-Type: text/plain; charset="us-ascii" We have been using a round double mailer with a metal insert from Fisher Scientific. The size for the outside cardboard mailer is 2 3/8" by 5 3/4 inch. Fisher has discontinued this mailer. Does anyone know a reasonable company that would carry these mailers. I found a company that has a similar product that is larger, but our cost for mailing will increase to mail the containers. I would appreciate any help. Thanks ------------------------------ Message: 20 Date: Thu, 04 Feb 2010 09:15:19 -0500 From: "Percival Karen" Subject: RE: [Histonet] Tape/Film Coverslips vs. Glass To: , , "Pyse Cynthia" Message-ID: <4B6A902702000011001F84DC@gv01a67m.gv.us.pri.wyeth.com> Content-Type: text/plain; charset=US-ASCII I've used both over the years, and I love the tape coverslippers. The slides dry very quickly because there is no mounting media step. The tape sticks to the slide via a chemical reaction between the xylene and the tape. One thing to remember with tape, you cannot use recycled xylene as your last xylene step before coverslipping. If I remember correctly, you cannot use xylene substitutes either. It's been a while, so that may have changed. The tape coverslips are easily removed with acetone. The pathologists loved it because they received their slides immediately. We didn't have to allow drying time before handing them in. I didn't find any tissue fading over time, either. Just my opinion. Good luck. >>> "Cynthia Pyse" 2/3/2010 9:21 AM >>> Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy.Crumpton@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?&nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Thu, 4 Feb 2010 10:05:30 -0500 From: rgrow@bmnet.com Subject: [Histonet] Re: Eosin leaching out of sections To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Scott, Your discription of eosin leaching out after a few days is classic for the possibility of bleach contamination on your staining containers. There are multiple possibilities of other causes, but this could be a simple cause, easily solved. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) _______________________________________________ ------------------------------ Message: 22 Date: Thu, 4 Feb 2010 10:19:43 -0500 From: "Sherwood, Margaret " Subject: Re: [Histonet] TRAP Assay and IHC To: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23FDE@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" I want to thank everyone who responded to my inquiry re: TRAP Assay. I don't think I made my request that clear re: IHC. We want to do both TRAP Assay and IHC (separately, of course) on mouse tibia. We would like to use frozen sections of mouse tibia for IHC. Our initial try to cut tibia on the cryostat has resulted in less than optimal sections. It appears that the tissue is lifting out of the section. In re: IHC on frozens - 1) do you treat the tibia before embedding in OCT? 2) what knife do you cut with on the cryostat? 3) do you fix slides with cold acetone before the IHC procedure (we ususally do)? Thanks again! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 23 Date: Thu, 4 Feb 2010 09:18:54 -0600 From: "Rosa Fields" Subject: RE: [Histonet] Tape/Film Coverslips vs. Glass To: "Percival Karen" , , , "Pyse Cynthia" Message-ID: <07732CE52EC3174AB891DE1C62DB4D8FC7B6A3@GIEXCHANGE.gidocs.net> Content-Type: text/plain; charset="iso-8859-1" The tape cover slippers are quicker, easier to manage the slides, but the cover slips pop off over time; the glass cover slippers are a bit temperamental; the slides take longer to dry, and the quality is better, and the cover slip stays on. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Percival Karen Sent: Thursday, February 04, 2010 8:15 AM To: histonet@lists.utsouthwestern.edu; Cathy.Crumpton@tuality.org; Pyse Cynthia Subject: RE: [Histonet] Tape/Film Coverslips vs. Glass I've used both over the years, and I love the tape coverslippers. The slides dry very quickly because there is no mounting media step. The tape sticks to the slide via a chemical reaction between the xylene and the tape. One thing to remember with tape, you cannot use recycled xylene as your last xylene step before coverslipping. If I remember correctly, you cannot use xylene substitutes either. It's been a while, so that may have changed. The tape coverslips are easily removed with acetone. The pathologists loved it because they received their slides immediately. We didn't have to allow drying time before handing them in. I didn't find any tissue fading over time, either. Just my opinion. Good luck. >>> "Cynthia Pyse" 2/3/2010 9:21 AM >>> Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy.Crumpton@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?&nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 5 *************************************** From Edina.Paal <@t> va.gov Thu Feb 4 09:54:30 2010 From: Edina.Paal <@t> va.gov (Paal, Edina E.) Date: Thu Feb 4 09:56:51 2010 Subject: [Histonet] BioCare immunostainer Message-ID: <7193324353F58A41A2B8BCD0957387F905327DB9@VHAV05MSGA1.v05.med.va.gov> We are at the verge of replacing our old immunostainer. We saw an excellent introduction to BioCare but I would like to hear from real people using it. Anything that makes you happy or unhappy? All information would be appreciated. Edina Paal, M.D. Pathologist VA Medical Center, Washington DC 50 Irving St, N.W., Rm GB205 Washington, DC 20422 Tel: (202) 518-4619 Fax: (202) 745-8284 From godsgalnow <@t> aol.com Thu Feb 4 10:04:19 2010 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Feb 4 10:03:57 2010 Subject: [Histonet] BioCare immunostainer Message-ID: <1752661513-1265299432-cardhu_decombobulator_blackberry.rim.net-782823543-@bda112.bisx.prod.on.blackberry> I have 3 of them in our lab and we use them like crazy, plus the support from the company is great. Roxanne ------Original Message------ From: Paal, Edina E. Sender: histonet-bounces@lists.utsouthwestern.edu To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] BioCare immunostainer Sent: Feb 4, 2010 10:54 AM We are at the verge of replacing our old immunostainer. We saw an excellent introduction to BioCare but I would like to hear from real people using it. Anything that makes you happy or unhappy? All information would be appreciated. Edina Paal, M.D. Pathologist VA Medical Center, Washington DC 50 Irving St, N.W., Rm GB205 Washington, DC 20422 Tel: (202) 518-4619 Fax: (202) 745-8284 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent from my Verizon Wireless BlackBerry From lblazek <@t> digestivespecialists.com Thu Feb 4 10:26:52 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Feb 4 10:27:19 2010 Subject: [Histonet] RE: BioCare immunostainer In-Reply-To: <7193324353F58A41A2B8BCD0957387F905327DB9@VHAV05MSGA1.v05.med.va.gov> References: <7193324353F58A41A2B8BCD0957387F905327DB9@VHAV05MSGA1.v05.med.va.gov> Message-ID: <5A2BD13465E061429D6455C8D6B40E390E976D9146@IBMB7Exchange.digestivespecialists.com> I have had BioCare's immunostainer the Intellipath for over a year now and am very happy with it. If there is anything I can answer for you feel free to contact me directly. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paal, Edina E. Sent: Thursday, February 04, 2010 10:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] BioCare immunostainer We are at the verge of replacing our old immunostainer. We saw an excellent introduction to BioCare but I would like to hear from real people using it. Anything that makes you happy or unhappy? All information would be appreciated. Edina Paal, M.D. Pathologist VA Medical Center, Washington DC 50 Irving St, N.W., Rm GB205 Washington, DC 20422 Tel: (202) 518-4619 Fax: (202) 745-8284 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kelleydurden <@t> pathology.ufl.edu Thu Feb 4 10:41:02 2010 From: kelleydurden <@t> pathology.ufl.edu (Durden, Kelley) Date: Thu Feb 4 10:42:33 2010 Subject: [Histonet] Superfrost plus gold slides Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE576EB368@HSC-CMS01.ad.ufl.edu> I've used superfrost plus gold slides - for frozen and paraffin - and they don't seem any better than the standard superfrost slides. Are you warming your slides in the oven before deparaffinizing? What temp and how long? We warm ours for 15 minutes in 56 degree oven. While in the military funds weren't available for special slides so we used gelatin in our water bath and never had anything fall off. Another option anyway. Amount of gelatin depends on gelatin "bloom." Not sure about the specs on that - quite a few years ago. Hope it helps Kelley From kelleydurden <@t> pathology.ufl.edu Thu Feb 4 11:03:14 2010 From: kelleydurden <@t> pathology.ufl.edu (Durden, Kelley) Date: Thu Feb 4 11:03:44 2010 Subject: [Histonet] ASCP Certified HT moving to Florida Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE576EB36A@HSC-CMS01.ad.ufl.edu> A list of VA jobs - a lot of info listed and will automatically update you as seldom or as often as you'd like. AmericaJob.com jobs@americajob.com Kelley Durden Senior Laboratory Technician Pathology, Immunology and Laboratory Medicine Molecular Pathology Core 1600 SW Archer Road PO Box 100275 Gainesville, FL 32610 (352) 273-7751 From kristenhinkle <@t> yahoo.com Thu Feb 4 10:49:39 2010 From: kristenhinkle <@t> yahoo.com (Kristen Reynolds) Date: Thu Feb 4 11:04:02 2010 Subject: [Histonet] Looking for job opportunity Message-ID: <427160.66100.qm@web50002.mail.re2.yahoo.com> Histonetters, I'm looking to find a position as a histology supervisor or histotech. I have 8 years of histo experience and 2 years supervisory experience in the clinical setting. If you know of any opportunities in the areas of Denver, Kansas City, Omaha/Lincoln, Cedar Rapids, Milwaukee/Madison please let me know. Thanks for your help! Kristen Reynolds kristenhinkle@yahoo.com From rjbuesa <@t> yahoo.com Thu Feb 4 11:13:12 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 4 11:13:25 2010 Subject: [Histonet] waterbaths In-Reply-To: Message-ID: <638784.13456.qm@web65702.mail.ac4.yahoo.com> The staining is an "after water bath" event not influenced by it. High temp. in the WB (above 55?C) will affect the integrity of the section, but not its staining. Ren? J. --- On Thu, 2/4/10, Pardue, Judith wrote: From: Pardue, Judith Subject: [Histonet] waterbaths To: histonet@lists.utsouthwestern.edu Date: Thursday, February 4, 2010, 10:38 AM Does anyone know if a waterbath temp is set to low ( 37C degrees) if the staining will be effected by this. Judith Pardue Memorial Healthcare System Chattanooga,Tn. judith_pardue@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential.? If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited.? If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kelleydurden <@t> pathology.ufl.edu Thu Feb 4 11:15:16 2010 From: kelleydurden <@t> pathology.ufl.edu (Durden, Kelley) Date: Thu Feb 4 11:19:48 2010 Subject: [Histonet] Experience with Tissue Tek VIP6 processor Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE576EB36B@HSC-CMS01.ad.ufl.edu> To Kathy & Kim, Just went out to CA for Sakura training on the VIP. We love our VIP 6 processor and the training they sent me for was phenomenal. We purchased this model and became somewhat familiar with it on our own. Then I got to go for the training and learned so much more. It has so many user friendly features. Lots of capabilities to reduce exposure to xylene, etc. "Solution Manager" so solutions never run low. In unit Bulk reservoir to store additional EtOH and xylene. No problems whatsoever. Spoke with others that went for training and their experiences were all positive. I've already recommended this unit to another lab here in Florida and would recommend it to anyone else. Small volume, large volume, minute specimens, large specimens, sensitive tissue, hearty tissue - it doesn't matter. This processor can accommodate them all. Hope this helps! Kelley From jkiernan <@t> uwo.ca Thu Feb 4 11:25:31 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Feb 4 11:25:36 2010 Subject: [Histonet] autofluorescence experiment Message-ID: I don't know any argument against using both copper sulphate and Sudan black B, and you have shown that the combination reduces the autofluorescence of bone. It's interesting that both are applied after immunofluorescent staining and are reported to cause some reduction of the desired fluorescence (Schnell et al 1999 J. Histochem. Cytochem. 47:719-730; Baschong et al 2001 J. Histochem. Cytochem. 449:1565-1571). Earlier chemical methods for suppressing autofluorescence involved pre-treatment of sections with either a heavy metal compound or a non-fluorescent dye. 0.2% osmium tetroxide for 5 mins is very effective; needs hours in slowly running tap water to wash it out before fluorescent staining. The dye Direct blue 1 (CI 24410), also known as Chicago sky blue B, Niagara blue 6B and pontamine sky blue, was recommended by Cowen et al 1985 Histochemistry 82:205-208 and Kutvolgyi et al 2006 Biotech. Histochem. 81:4-6. Cowen et al used a 0.05% solution of the dye in PBS with 1% DMSO. I haven't treid it myself. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicole Collette Date: Wednesday, February 3, 2010 19:12 Subject: [Histonet] autofluorescence experiment To: Histonet@lists.utsouthwestern.edu > Hello, All, > > I am working on doing some IF stains with bone samples (lucky > me!). I > am having a difficult time sometimes to assess the antibody > since the > autofluorescence gets in the way. I am using undecalcified, FFPE > sections (late embryo and neonate mouse bones). Without > treatment, I > see autofluorescence everywhere, but most frustrating is the red > blood cells and the mineralized matrix. It took me a while to > get > samples that are properly fixed, section well, and stay on the > slides, so I am not particularly jazzed about messing with the > fixation protocol. Thus, I conducted an experiment today of > several > published and voodoo methods to reduce autofluorescence with > samples > that did NOT undergo the IF protocol : > > no treatment > photobleaching, fluorescent light box, up to 2 weeks > photobleaching, UV crosslinking light, 2 inches from source, 24h > 10mM copper sulfate in 50mM ammonium acetate, pH 5.0 > 0.25% (v/v) NH3 in 70% ethanol (in water) > 0.25% (v/v) NH4OH in 70% ethanol (in water) (since it was > unclear > from the published reference what the source of ammonia was, and > I > have made this mistake before on some other thing) > 0.3% (w/v) Sudan Black in 70% ethanol (in water) > > I found that the most effective treatment in my hands is Sudan > Black > for cell-based autofluorescence, but it did not seem to impact > the > autofluorescence from the mineralized matrix at all, while > copper > sulfate had significant impact on the autofluorescence in > mineralized > bone, but did not quench cell-based autofluorescence as well as > the > Sudan Black (it had an even but incomplete impact over the > entire > section). I have tried Sudan Black on my slides before, and have > found that it did not seem to interfere significantly with the > antibody signal. I modified the Sudan Black protocol to > eliminate the > "goopies" and "chunkies" resulting on my slides from previous > attempts, and am happy with the results- a light, even stain. > > My question to all you chemistry folks: Is there some reason why > copper sulfate treatment followed by washing and subsequent > Sudan > Black treatment (and more washing) cannot or should not be used? > I > tried them together on my unstained slides, they look pretty > darn > fabulous. Just striving for clean data and beautiful pictures. > > Thanks again for all your help, > Sincerely, > > Nicole Collette > Lawrence Livermore National Lab/ UC Berkeley > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rick.Garnhart <@t> memorialhealthsystem.com Thu Feb 4 11:46:12 2010 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Thu Feb 4 11:46:17 2010 Subject: [Histonet] RE:slide Labels In-Reply-To: Message-ID: We are using a thermo transfer slide label from Electronic Imaging Materials. We use them through HIER and Microwave Fungus procedure without difficulty. They are reasonability priced and are xylene and chemical resistant. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From kelleydurden <@t> pathology.ufl.edu Thu Feb 4 11:45:56 2010 From: kelleydurden <@t> pathology.ufl.edu (Durden, Kelley) Date: Thu Feb 4 11:46:26 2010 Subject: [Histonet] xylene alternatives Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE576EB36C@HSC-CMS01.ad.ufl.edu> Nothing works as well as xylene. We have tried several different substitutes and nothing has provided consistent results. Xylene has a slight tolerance for water. Unlike xylene substitutes. If any water is present in the substitute before coverslipping the results are disastrous. So unless you have a very small volume or change your pre xylene alcohols several times a day you may end up with sub standard results. Plus substitutes are more expensive - generally. Kelley From Rick.Garnhart <@t> memorialhealthsystem.com Thu Feb 4 11:47:08 2010 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Thu Feb 4 11:47:16 2010 Subject: [Histonet] Slide Drying In-Reply-To: Message-ID: Anyone out there using microwave to dry H+E slides? How Long and what staining racks. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From jaylundgren <@t> gmail.com Thu Feb 4 11:53:59 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Feb 4 11:54:07 2010 Subject: [Histonet] xylene alternatives In-Reply-To: <92E6B93E0A3D544C87DDDE33E7608AAE576EB36C@HSC-CMS01.ad.ufl.edu> References: <92E6B93E0A3D544C87DDDE33E7608AAE576EB36C@HSC-CMS01.ad.ufl.edu> Message-ID: When you add in the cost of disposing of your xylene, or the cost of distilling it (including tech time), xylene substitutes come closer to the cost of xylene. Jay A. Lundgren M.S., HTL (ASCP) On Thu, Feb 4, 2010 at 12:45 PM, Durden, Kelley < kelleydurden@pathology.ufl.edu> wrote: > Nothing works as well as xylene. We have tried several different > substitutes and nothing has provided consistent results. Xylene has a > slight tolerance for water. Unlike xylene substitutes. If any water is > present in the substitute before coverslipping the results are disastrous. > So unless you have a very small volume or change your pre xylene alcohols > several times a day you may end up with sub standard results. Plus > substitutes are more expensive - generally. > > Kelley > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From trathborne <@t> somerset-healthcare.com Thu Feb 4 11:59:55 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Feb 4 12:00:27 2010 Subject: [Histonet] xylene alternatives In-Reply-To: Message-ID: Why? They should both be disposed of in the same way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jay Lundgren Sent: Thursday, February 04, 2010 12:54 PM To: Durden, Kelley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] xylene alternatives When you add in the cost of disposing of your xylene, or the cost of distilling it (including tech time), xylene substitutes come closer to the cost of xylene. Jay A. Lundgren M.S., HTL (ASCP) On Thu, Feb 4, 2010 at 12:45 PM, Durden, Kelley < kelleydurden@pathology.ufl.edu> wrote: > Nothing works as well as xylene. We have tried several different > substitutes and nothing has provided consistent results. Xylene has a > slight tolerance for water. Unlike xylene substitutes. If any water is > present in the substitute before coverslipping the results are disastrous. > So unless you have a very small volume or change your pre xylene alcohols > several times a day you may end up with sub standard results. Plus > substitutes are more expensive - generally. > > Kelley > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From anonwums1 <@t> gmail.com Thu Feb 4 12:02:37 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Feb 4 12:02:44 2010 Subject: [Histonet] TRAP Assay and IHC In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23FDE@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23FDE@PHSXMB30.partners.org> Message-ID: <858249121002041002j8a6f0c9ubf4b6b2526fe491@mail.gmail.com> I'm by no means an expert for cutting frozen mouse bones, but I've done it. It's certainly more difficult than other tissues, but you can get decent sections with lots of practice and patience. When I've gotten it to work, the bones were fixed in formalin or PFA, decalcified in EDTA, cryoprotected in 30% sucrose, and then snap frozen in OCT using a beaker of 2-methylbutane sitting in an ice bucket of liquid nitrogen. The knives we use are nothing special. I don't post-fix after IHC and usually the sections stick to Superfrost Plus Slides if you let them dry completely before staining. I'm not entirely sure what you mean by the tissue lifting out of the section. Sometimes, the marrow can spill out of the bones or the decalcified bone can fall off and either pull away from the endosteum or even completely fall off and splay across the section. More often, the periosteum will pull away from the OCT a bit. I've found the best way to avoid this is to flatten the section with a paintbrush the best you can, and then quickly and firmly plant the slide onto the section. I hold the slide on each between my index fingers and thumbs and slowly lower it until it almost touches. Then I press down rapidly so the entire section should get pressed onto the slide as quickly as possible. If you go slowly and allow the section to pull itself up onto the slide in increments, you'll often get falling off. I hope that helps, Adam On Thu, Feb 4, 2010 at 9:19 AM, Sherwood, Margaret wrote: > I want to thank everyone who responded to my inquiry re: TRAP Assay. I > don't > think I made my request that clear re: IHC. We want to do both TRAP Assay > and > IHC (separately, of course) on mouse tibia. > > We would like to use frozen sections of mouse tibia for IHC. Our initial > try to > cut tibia on the cryostat has resulted in less than optimal sections. It > appears that the tissue is lifting out of the section. > In re: IHC on frozens - 1) do you treat the tibia before embedding in > OCT? > 2) what knife do you cut with on the cryostat? 3) do you fix slides with > cold > acetone before the IHC procedure (we ususally do)? > > > Thanks again! > > Peggy > > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDW 214) > Massachusetts General Hospital > 55 Fruit Street > Boston, Massachusetts 02114-2696 > 617-724-4839 (voice mail) > 617-726-6983 (lab) > 617-726-1206 (fax) > msherwood@partners.org > > > > The information in this e-mail is intended only for the person to whom it > is > addressed. If you believe this e-mail was sent to you in error and the > e-mail > contains patient information, please contact the Partners Compliance > HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in > error > but does not contain patient information, please contact the sender and > properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gmartin <@t> marshallmedical.org Thu Feb 4 12:19:49 2010 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Thu Feb 4 12:19:56 2010 Subject: [Histonet] Bone Marrow clot Message-ID: <6ED9D4252F278841A0593D3D788AF24C07B5412E@mailsvr.MARSHMED.local> We have recently been receiving bone marrow specimens where the syringe has been heparinized before the aspiration is retrieved. We are having problem getting the clot sections to stay on the slide. Could the heparinization be causing this problem, and if so, is there a solution? Thank you Gary From cory.collins <@t> DHAT.com Thu Feb 4 12:28:14 2010 From: cory.collins <@t> DHAT.com (Cory Collins) Date: Thu Feb 4 12:28:20 2010 Subject: [Histonet] Pathology Laboratory Manager Opening in Houston, Texas Message-ID: SightLine is expanding their service lines and is seeking a pathology laboratory manager who will be responsible for the pathology laboratory services from the design and construction of the new facility, equipment purchasing, hiring staff, policies and procedures, certification and licensure. The ideal candidate must have managed laboratory staff and have been through CLIA certification. They must also have the ability to step-in as a laboratory technician should the situation demanded it. The new facility will be located on South Main near the Reliant Stadium in Houston, Texas Reports to Vice President of Operations. Education: Bachelor Degree in Science with appropriate on the job training. Please indicate your interest by sending emails with resumes attached to hr@sightlinehealth.com From tjay30 <@t> yahoo.com Thu Feb 4 12:28:27 2010 From: tjay30 <@t> yahoo.com (Timothy Jay) Date: Thu Feb 4 12:28:30 2010 Subject: [Histonet] GI, Uro, or Derm Path Lab Set Up Message-ID: <657193.86909.qm@web34305.mail.mud.yahoo.com> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tjay30@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. ? Timothy Garcia-Jay, MHA From blebl2 <@t> lsuhsc.edu Thu Feb 4 12:35:31 2010 From: blebl2 <@t> lsuhsc.edu (Leblanc, Barbara Ann) Date: Thu Feb 4 12:35:52 2010 Subject: [Histonet] CPT code 88313 on non-gynecological cases Message-ID: <751F3180611527449849CB9B710A7BA6FAF5C3@UMC-EXCH04.master.lsuhsc.edu> Hello to all: On non-gyn cases which have smears stained with Diff-Quik, are you charging 88313 (special stain) for the Diff-Quik. Our hospital coder insists that we can and should be charging this on all non-gyn cases when a Diff-Quik stain is performed, besides the routine normal charges. Thanks for your input. Barbara LSUMC Histology Lafayette, LA From Wanda.Smith <@t> HCAhealthcare.com Thu Feb 4 12:39:58 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Thu Feb 4 12:40:06 2010 Subject: [Histonet] RE: CPT code 88313 on non-gynecological cases In-Reply-To: <751F3180611527449849CB9B710A7BA6FAF5C3@UMC-EXCH04.master.lsuhsc.edu> References: <751F3180611527449849CB9B710A7BA6FAF5C3@UMC-EXCH04.master.lsuhsc.edu> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1384BA2AD2@NADCWPMSGCMS03.hca.corpad.net> Yes, that is correct for Diff Quik stains on Non-gyn specimens. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leblanc, Barbara Ann Sent: Thursday, February 04, 2010 1:36 PM To: histonet@pathology.swmed.edu Subject: [Histonet] CPT code 88313 on non-gynecological cases Hello to all: On non-gyn cases which have smears stained with Diff-Quik, are you charging 88313 (special stain) for the Diff-Quik. Our hospital coder insists that we can and should be charging this on all non-gyn cases when a Diff-Quik stain is performed, besides the routine normal charges. Thanks for your input. Barbara LSUMC Histology Lafayette, LA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KPercival <@t> wyeth.com Thu Feb 4 12:57:48 2010 From: KPercival <@t> wyeth.com (Percival Karen) Date: Thu Feb 4 12:57:59 2010 Subject: [Histonet] Bone Marrow clot In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C07B5412E@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C07B5412E@mailsvr.MARSHMED.local> Message-ID: <4B6AD25C02000011001F856A@gv01a67m.gv.us.pri.wyeth.com> Gary, absolutely the heparin is the problem. We (histologist) would participate in the bone marrow procedure to make slides immediately with the unheparinized sample. If someone is making those slides for you, they need to use an unheparinized specimen. Of course, that makes the task of slide preparation more difficult, as the time is very limited. It helps to have an experienced person performing that procedure. Karen >>> "Martin, Gary" 2/4/2010 1:19 PM >>> We have recently been receiving bone marrow specimens where the syringe has been heparinized before the aspiration is retrieved. We are having problem getting the clot sections to stay on the slide. Could the heparinization be causing this problem, and if so, is there a solution? Thank you Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlschneider <@t> gmail.com Thu Feb 4 13:19:10 2010 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Thu Feb 4 13:19:16 2010 Subject: [Histonet] RE: CPT code 88313 on non-gynecological cases In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1384BA2AD2@NADCWPMSGCMS03.hca.corpad.net> References: <751F3180611527449849CB9B710A7BA6FAF5C3@UMC-EXCH04.master.lsuhsc.edu> <9E2D36CE2D7CBA4A94D9B22E8328A3BA1384BA2AD2@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <1085e7001002041119p154d8953hf0e373aa6d216561@mail.gmail.com> Can you cite some authority or reference on this? I'm very interested in the answer. This issue came up a year or so ago in our practice. It was determined that we couldn't charge extra for the Diff-Quik, but I'm not sure I understood why not. I was trained to interpret all thyroid FNA's with both the usual Pap stain, as well as a DQ, so it's not at all a trivial number of cases. My colleagues seemed OK with interpreting them with Pap stain only, since we'd have to eat the cost on the DQ. Or do we? If we can bill 88313 for the DQ on thyroid FNA's, then we will probably go back to doing it. Dan Schneider On Thu, Feb 4, 2010 at 12:39 PM, Smith Wanda wrote: > Yes, that is correct for Diff Quik stains on Non-gyn specimens. > Wanda > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error > and that any use, dissemination, distribution, forwarding, printing, or > copying of this email or any attached files is strictly prohibited. If you > have received this email in error, please immediately purge it and all > attachments and notify the sender by reply email or contact the sender at > the number listed. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leblanc, Barbara > Ann > Sent: Thursday, February 04, 2010 1:36 PM > To: histonet@pathology.swmed.edu > Subject: [Histonet] CPT code 88313 on non-gynecological cases > > Hello to all: > > On non-gyn cases which have smears stained with Diff-Quik, are you charging > 88313 (special stain) for the Diff-Quik. Our hospital coder insists that we > can and should be charging this on all non-gyn cases when a Diff-Quik stain > is performed, besides the routine normal charges. > Thanks for your input. > > Barbara > LSUMC Histology > Lafayette, LA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Lynne.Bell <@t> cvmc.org Thu Feb 4 13:58:43 2010 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Thu Feb 4 13:58:49 2010 Subject: [Histonet] RE: CPT code 88313 on non-gynecological cases In-Reply-To: <1085e7001002041119p154d8953hf0e373aa6d216561@mail.gmail.com> Message-ID: I would be very interested in a reference on this as well. We do use Diff-Quik stains for air-dried FNA slides. It would be great if we could charge for them!! Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From annigyg <@t> gmail.com Thu Feb 4 14:09:40 2010 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Feb 4 14:09:49 2010 Subject: [Histonet] RE: CPT code 88313 on non-gynecological cases In-Reply-To: References: <1085e7001002041119p154d8953hf0e373aa6d216561@mail.gmail.com> Message-ID: you are billing for the final report and if in your lab you determine that this involves a PAP and a DQ on all your NGs then that is all included in the cost the same is true of an 88305 surgical which includes 3/4 H&Es as decided by the lab - its the final report which is being charged for 88313/88312's are for the add-on specials just my 5c worth AbuDhabiAnnie On 4 February 2010 21:58, Bell, Lynne wrote: > I would be very interested in a reference on this as well. We do use > Diff-Quik stains for air-dried FNA slides. It would be great if we could > charge for them!! > > Lynne A. Bell, HT (ASCP) > Technical Specialist, Histology > Central Vermont Medical Center > 130 Fisher Road > Barre, VT 05641 > 802-371-4923 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From lpaveli1 <@t> hurleymc.com Thu Feb 4 14:18:46 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Feb 4 14:19:06 2010 Subject: [Histonet] Bone Marrow clot In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C07B5412E@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C07B5412E@mailsvr.MARSHMED.local> Message-ID: <4B6AE555.59CD.00EE.0@hurleymc.com> One of our physicians also uses this method of heparin when obtaining their BM's. To help it clot, we make up a solution of formaldehyde and methanol. Using the same syringe, pull some of the formal methanol into the syringe and let it sit.....say 10 minutes to give it time to clot. Then, take the plunger out of the syringe and spin it down in the centrifuge to compact the specimen. It usually turns out to be a larger specimen than the usual clot, but the method works well. The pathologists' don't have any problems with this method either. (yea!) Below is the solution of formal methanol that we use. 1 part 37% formaldehyde 9 parts 100% methanol hope this helps! Lynette From Rcartun <@t> harthosp.org Thu Feb 4 14:28:56 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Feb 4 14:29:04 2010 Subject: [Histonet] GI, Uro, or Derm Path Lab Set Up In-Reply-To: <657193.86909.qm@web34305.mail.mud.yahoo.com> References: <657193.86909.qm@web34305.mail.mud.yahoo.com> Message-ID: <4B6AE7B7.7400.0077.1@harthosp.org> We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are "Cherry-picking" the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Timothy Jay 2/4/2010 1:28 PM >>> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tjay30@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. Timothy Garcia-Jay, MHA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Feb 4 14:35:16 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Feb 4 14:35:21 2010 Subject: [Histonet] Bone Marrow clot In-Reply-To: <4B6AE555.59CD.00EE.0@hurleymc.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D3B@is-e2k3.grhs.net> Nice tip! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, February 04, 2010 2:19 PM To: histonet@lists.utsouthwestern.edu; Gary Martin Subject: Re: [Histonet] Bone Marrow clot One of our physicians also uses this method of heparin when obtaining their BM's. To help it clot, we make up a solution of formaldehyde and methanol. Using the same syringe, pull some of the formal methanol into the syringe and let it sit.....say 10 minutes to give it time to clot. Then, take the plunger out of the syringe and spin it down in the centrifuge to compact the specimen. It usually turns out to be a larger specimen than the usual clot, but the method works well. The pathologists' don't have any problems with this method either. (yea!) Below is the solution of formal methanol that we use. 1 part 37% formaldehyde 9 parts 100% methanol hope this helps! Lynette _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 4 14:42:26 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 4 14:42:30 2010 Subject: [Histonet] Slide Drying In-Reply-To: Message-ID: <751174.69348.qm@web65713.mail.ac4.yahoo.com> You should not "dry" slides in the MW. The paraffin in the sections is "transparent" to microwaves and what you are going to do is locally heat (and I mean heat) the remaining water underneath the sections but you are not going to really melt the paraffin. There is no real good substitute for a convection oven to dry the sections. Ren? J. --- On Thu, 2/4/10, Rick.Garnhart@memorialhealthsystem.com wrote: From: Rick.Garnhart@memorialhealthsystem.com Subject: [Histonet] Slide Drying To: histonet@lists.utsouthwestern.edu Date: Thursday, February 4, 2010, 12:47 PM Anyone out there using microwave to dry H+E slides? How Long and what staining racks. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph:? 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 4 14:44:33 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 4 14:44:37 2010 Subject: [Histonet] xylene alternatives In-Reply-To: Message-ID: <319708.85446.qm@web65707.mail.ac4.yahoo.com> There is no better alternative than not using xylene or any of its "so called" substitutes to get the best infiltration possible with the lowest infiltration gradient possible. Ren? J. --- On Thu, 2/4/10, Jay Lundgren wrote: From: Jay Lundgren Subject: Re: [Histonet] xylene alternatives To: "Durden, Kelley" Cc: "histonet@lists.utsouthwestern.edu" Date: Thursday, February 4, 2010, 12:53 PM When you add in the cost of disposing of your xylene, or the cost of distilling it (including tech time), xylene substitutes come closer to the cost of xylene. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Jay A. Lundgren M.S., HTL (ASCP) On Thu, Feb 4, 2010 at 12:45 PM, Durden, Kelley < kelleydurden@pathology.ufl.edu> wrote: > Nothing works as well as xylene.? We have tried several different > substitutes and nothing has provided consistent results.? Xylene has a > slight tolerance for water.? Unlike xylene substitutes.? If any water is > present in the substitute before coverslipping the results are disastrous. >? So unless you have a very small volume or change your pre xylene alcohols > several times a day you may end up with sub standard results.? Plus > substitutes are more expensive - generally. > > Kelley > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SFinley <@t> providencehealth.bc.ca Thu Feb 4 14:47:10 2010 From: SFinley <@t> providencehealth.bc.ca (Finley, Sue [PH]) Date: Thu Feb 4 14:47:20 2010 Subject: [Histonet] xylene alternatives In-Reply-To: <319708.85446.qm@web65707.mail.ac4.yahoo.com> References: <319708.85446.qm@web65707.mail.ac4.yahoo.com> Message-ID: <7D627F9CD9AE514087E447A9716D4D358AE7E625@vchexmbp14.vch.ca> Why not look at processing that excludes the use of xylene all together ie milestone pathos tissue processor. I am not a sales rep only a very happy end user Regards sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 04, 2010 12:45 PM To: KelleyDurden; Jay Lundgren Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] xylene alternatives There is no better alternative than not using xylene or any of its "so called" substitutes to get the best infiltration possible with the lowest infiltration gradient possible. Ren? J. --- On Thu, 2/4/10, Jay Lundgren wrote: From: Jay Lundgren Subject: Re: [Histonet] xylene alternatives To: "Durden, Kelley" Cc: "histonet@lists.utsouthwestern.edu" Date: Thursday, February 4, 2010, 12:53 PM When you add in the cost of disposing of your xylene, or the cost of distilling it (including tech time), xylene substitutes come closer to the cost of xylene. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Jay A. Lundgren M.S., HTL (ASCP) On Thu, Feb 4, 2010 at 12:45 PM, Durden, Kelley < kelleydurden@pathology.ufl.edu> wrote: > Nothing works as well as xylene.? We have tried several different > substitutes and nothing has provided consistent results.? Xylene has a > slight tolerance for water.? Unlike xylene substitutes.? If any water is > present in the substitute before coverslipping the results are disastrous. >? So unless you have a very small volume or change your pre xylene alcohols > several times a day you may end up with sub standard results.? Plus > substitutes are more expensive - generally. > > Kelley > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Thu Feb 4 14:56:53 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Feb 4 14:57:12 2010 Subject: [Histonet] GI, Uro, or Derm Path Lab Set Up In-Reply-To: <4B6AE7B7.7400.0077.1@harthosp.org> References: <657193.86909.qm@web34305.mail.mud.yahoo.com> <4B6AE7B7.7400.0077.1@harthosp.org> Message-ID: Especially when these physician hire unqualified people to run these labs and flood the histonet with their uneducated questions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 04, 2010 2:29 PM To: histonet@lists.utsouthwestern.edu; Timothy Jay Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are "Cherry-picking" the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Timothy Jay 2/4/2010 1:28 PM >>> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tjay30@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. Timothy Garcia-Jay, MHA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From araniqkslvr <@t> yahoo.com Thu Feb 4 16:03:48 2010 From: araniqkslvr <@t> yahoo.com (Paula) Date: Thu Feb 4 16:04:52 2010 Subject: [Histonet] Looking for Position in NC Message-ID: <715237.37609.qm@web30304.mail.mud.yahoo.com> Hello, I recently moved to NC and I'm looking for a histo positon. Entry level tech or assistant. I can work 1st or 3rd shift. I am a registered HT but need to update my experience. I am in Zebulon, NC. I have priced books and am willing to update my schooling, but cannot take classes for a year, as in state tuition requirements require you to be here for a year before a person can obtain in-state status. Paula From azdudley <@t> hotmail.com Thu Feb 4 16:17:50 2010 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu Feb 4 16:17:53 2010 Subject: [Histonet] cleaning cryostat Message-ID: we are coming up for our cap inspection and I need to write the procedure over for cleaning the cryostat once a week?????? I think it is overkill to take it down every week but that is what it says. what is everyone else doing and what do you use to disinfect it? we now every week wipe it with 70% alc. but we do not take it all down. I know we have had things on about this before but just wanted to update. thanks so much anita dudley providence hosp mobile alabama _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/ From KMB01 <@t> grh.org Thu Feb 4 16:55:58 2010 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Thu Feb 4 16:56:03 2010 Subject: [Histonet] cleaning cryostat In-Reply-To: Message-ID: I would like this information also. This is the first year we are CAP so any help I can get would be appreciated. Thanks so much have a great Friday and Week end. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Thursday, February 04, 2010 2:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning cryostat we are coming up for our cap inspection and I need to write the procedure over for cleaning the cryostat once a week?????? I think it is overkill to take it down every week but that is what it says. what is everyone else doing and what do you use to disinfect it? we now every week wipe it with 70% alc. but we do not take it all down. I know we have had things on about this before but just wanted to update. thanks so much anita dudley providence hosp mobile alabama _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Thu Feb 4 18:01:49 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Feb 4 18:01:55 2010 Subject: [Histonet] GI, Uro, or Derm Path Lab Set Up References: <657193.86909.qm@web34305.mail.mud.yahoo.com> <4B6AE7B7.7400.0077.1@harthosp.org> Message-ID: <90354A475B420441B2A0396E5008D49692BEFF@copc-sbs.COPC.local> I am in total agreement with both Richard and Felton here. Sorry Timothy if you are reading this but to me the bottom line is patient care. And in the end set-ups like this hurt patients. These "labs" and I'm using the term loosely are put in place to line the pockets of a small number of people at the top of a pyramid. I guess someone will always work to put things like this together if there is a demand. But please realize, if this is what you're involved in, the reality is... ~ Questionable facilities - Cramming professional, OSHA legal and ergonomically correct lab operations into small office spaces is a poor idea at best. Proper plumbing, ventilation and lab space are serious considerations and fall by the wayside when these operations are put together. ~ Qualified personnel - All of us posting on this list understand the fact that qualified, competent Histology Lab personnel are in demand and difficult to find. The wage for qualified, competent technical expertise does not fit into the plans of the originators of these operations. ~ Proper equipment and instruments - While the aim of these operations is limited in scope, basic functional equipment and instruments are required. In the overall scheme of things, pathology is still a bargain. However, money must be spent to properly equip a competent working lab with modern instruments. I could go on but that makes the basic point. And Timothy, lest you think that I'm shooting from the hip I assure I am not. Like most people on this list, I am a serious, diligent professional. I have interviewed people from "pod" labs that have worked under horrible conditions. I am also aware of a "pod" lab trying to get off the ground that shouldn't have an ice cube's chance in hell. The plans for this lab do not have proper plumbing, ventilation or space and there is no equipment. While a position has been advertised I cannot think of a single tech, worth his or her salt, that would consider associating themselves with such an ill-conceived venture. One last point - don't forget Timothy, that one day you may be that patient. Or it may be your mother, sister, daughter, wife, son, friend...I'll stop there. Every block, every slide, every piece of tissue that I or any of my staff encounter is a precious patient...a human life. So trying to dumb everything down, cut corners and make a small number of people wealthy in the process is an irresponsible and utterly ridiculous risk. The professionals in our field have worked hard for a good number of years to come out of the basement spaces and after-thought little nooks and crannies and other poor facilities to do the highly skilled work this profession demands. I for one will never abide going backwards and I don't believe people of good conscience and humanity will either. Sincerely yours, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Thursday, February 04, 2010 12:57 PM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu; Timothy Jay Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Especially when these physician hire unqualified people to run these labs and flood the histonet with their uneducated questions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 04, 2010 2:29 PM To: histonet@lists.utsouthwestern.edu; Timothy Jay Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are "Cherry-picking" the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Timothy Jay 2/4/2010 1:28 PM >>> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tjay30@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. Timothy Garcia-Jay, MHA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sprice2003 <@t> gmail.com Thu Feb 4 18:48:23 2010 From: sprice2003 <@t> gmail.com (Sally Price) Date: Thu Feb 4 18:48:28 2010 Subject: [Histonet] RE: GI, Uro, or Derm Path Lab Set Up Message-ID: ...and another thing -- since when is it OK to post such blatant solicitations on the HistoNet? other vendors are chastised for this on a regular basis! Sally From rsrichmond <@t> gmail.com Thu Feb 4 20:14:50 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Feb 4 20:14:54 2010 Subject: [Histonet] Formula 83 Message-ID: Allison Hutton, HTL(ASCP)cm, Lead Tech Histology, Doylestown Hospital, Doylestown, Pennsylvania asks: >>For those of you who are using Formula 83, could you please contact me off line? We have completed our demo but still have a few questions.<< Formula 83, offered by CBG Biotech Ltd., is a napthenic (cycloalkane) hydrocarbon used as a xylene substitute. It differs in structure from the aliphatic hydrocarbons such as Richard Allan's Clear-rite 3. It's in use in a lab I'm working in at the moment. I have two reservations about it. The first is that, unlike the aliphatics, it has a definite odor, which could be objectionable in a confined space. The second reservation: Formula 83 has a flash point of only 7 degrees Fahrenheit, lower than xylene (78 degrees) and other aliphatics, some of which have flash points as high as 144 F. The fire hazard here is worth considering. Formula 83 has been discussed before on Histonet, and the posts are worth looking up. When purchasing a new aliphatic, there are several important considerations. Your purchasing people need to understand that they can't change the xylene substitute on you, just because something a bit cheaper comes along. If you recycle, every different xylene substitute has a different distillation routine. It's important to ask everyone in the lab - in particular, the cytotechnologist is likely to have very strong opinions about the solvents they use in their ascending sequence before coverslipping. Bob Richmond Samurai Pathologist Knoxville TN From CIngles <@t> uwhealth.org Thu Feb 4 22:44:24 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Feb 4 22:49:16 2010 Subject: [Histonet] cleaning cryostat References: Message-ID: I work in a Mohs Lab. (high output frozen skin) We wipe out the cryostat with 95% ETOH and clear out the shavings every day we use our machines. We normally tear down and completely defrost our cryostats every other month or so, depending on humidity and frost build up. (and available time to have a cryostat down.) We are not inspected by CAP, but JCAHO and CLIA who are at least as stringent. Claire ________________________________ I would like this information also. This is the first year we are CAP so any help I can get would be appreciated. Thanks so much have a great Friday and Week end. Kathy Gorham H.T. From lscott <@t> sfcn.org Fri Feb 5 00:24:06 2010 From: lscott <@t> sfcn.org (Scott Hendricksen) Date: Fri Feb 5 00:24:15 2010 Subject: [Histonet] What slides do you use? Message-ID: Hi, I was wondering what microscope slides you use. For the last couple of years I have noticed that a lot of the different brand slides are dirty. We have used Erie brand slides for many years, and they are the best quality I can find. Now even a lot of the Erie slides are dirty and we end up throwing a few away. Any suggestions for good clean slides at a reasonable price? Thanks again ! Scott Hendricksen HT(ASCP) From lscott <@t> sfcn.org Fri Feb 5 00:35:33 2010 From: lscott <@t> sfcn.org (Scott Hendricksen) Date: Fri Feb 5 00:35:43 2010 Subject: [Histonet] New slide drier recommendations please Message-ID: <7A5A7B7C-59A0-4AA1-BDBA-09EAD2C11F75@sfcn.org> Hi, Does anyone have a suggestion for a forced air slide drier? We were looking at possibly the TBS SD-II-120. We have already tried a slide oven but it takes too long for the slides to dry, it was lacking a fan to circulate the air. Thanks, Scott Hendricksen HT(ASCP) From foreightl <@t> gmail.com Fri Feb 5 00:47:22 2010 From: foreightl <@t> gmail.com (Pat Laurie) Date: Fri Feb 5 00:53:22 2010 Subject: [Histonet] BioCare immunostainer In-Reply-To: <7193324353F58A41A2B8BCD0957387F905327DB9@VHAV05MSGA1.v05.med.va.gov> References: <7193324353F58A41A2B8BCD0957387F905327DB9@VHAV05MSGA1.v05.med.va.gov> Message-ID: We have an intellipath. It is a great machine. They have several unique innovations which makes customizing the work much easier. It depends what you need from the machine, but this had many innovations which helped our IHC department. It is also a completely open system which is becoming more and more difficult to find nowdays. On Thu, Feb 4, 2010 at 7:54 AM, Paal, Edina E. wrote: > We are at the verge of replacing our old immunostainer. We saw an > excellent introduction to BioCare but I would like to hear from real > people using it. Anything that makes you happy or unhappy? All > information would be appreciated. > > > Edina Paal, M.D. > Pathologist > VA Medical Center, Washington DC > 50 Irving St, N.W., Rm GB205 > Washington, DC 20422 > > Tel: (202) 518-4619 > Fax: (202) 745-8284 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com From foreightl <@t> gmail.com Fri Feb 5 02:12:43 2010 From: foreightl <@t> gmail.com (Pat Laurie) Date: Fri Feb 5 02:12:54 2010 Subject: [Histonet] Slide baking before IHC Message-ID: Histonet, I have heard rather anecdotally that if you bake slides at high temps (75 degrees to 80 degrees) before IHC for a long period of time (several hours to days), you may affect antigenicity for some antibodies. Has there been any study done about this? Also, what if it is a high temp (around 75 degrees C) for just 20 minutes? Or a low temperature for a long period of time? Thanks in advance for your input. -- Patrick Laurie HT(ASCP)QIHC plaurie@cellnetix.com From jaylundgren <@t> gmail.com Fri Feb 5 05:59:24 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Feb 5 05:59:31 2010 Subject: [Histonet] RE: GI, Uro, or Derm Path Lab Set Up In-Reply-To: References: Message-ID: These pod labs are a curse and make it that much harder for HTs and HTLs to get good paying, respected positions. Hospitals and reference labs are losing their biopsy workload to GI, GU, and Derm practices. Thomas is right. Some of these consultants leave copies of their credentials on the wall, and the practice then trains a histo monkey to turn out the work for $10./hr. When an inspection rolls around, oops, the HT is "on vacation", and the histo monkey goes back to sweeping the floors or filing, or whatever they were doing before, for the duration of the inspection. Pod labs are based on pure greed, and are part of the reason for spiralling health care costs in the U.S. Any histopathology technician or technologist that works for these consultants should realize that they are cutting their own throat in the long run. I notice that Timothy lists a Masters of Health Administration, but NOT an HT or HTL. Obviously he is familiar with health care economics, but that's not the whole picture. Money should never be the primary motivation in the practice of medicine. Sincerely, Jay A. Lundgren MS, HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Teri.Hallada <@t> midmichigan.org Fri Feb 5 11:20:01 2010 From: Teri.Hallada <@t> midmichigan.org (Teri.Hallada@midmichigan.org) Date: Fri Feb 5 11:20:16 2010 Subject: [Histonet] Temperature and Humidity Monitoring System Message-ID: <8839B08E3ED7364E8CBBD53882C984D5141029B6@MAILSRV01.midmichigan.net> Anyone recently price one of these for a small to medium lab? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From Timothy.Morken <@t> ucsfmedctr.org Fri Feb 5 11:23:34 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Feb 5 11:23:50 2010 Subject: [Histonet] Slide baking before IHC In-Reply-To: References: Message-ID: <1AAF670737F193429070841C6B2ADD4C0121DF474F@EXMBMCB15.ucsfmedicalcenter.org> Here is the reference: Effect of Slide Drying at 80Deg C on Immunohistochemistry A.F. Henwood J Histotechnology, VOl. 28, no. 1, March 2005 If you are an NSH member you can email the NSH office and ask for a pdf reprint. The author compares heating the slides at 80C for seven hours to one hour at 65C. Some antigens were adversely affected, some were not. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Friday, February 05, 2010 12:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide baking before IHC Histonet, I have heard rather anecdotally that if you bake slides at high temps (75 degrees to 80 degrees) before IHC for a long period of time (several hours to days), you may affect antigenicity for some antibodies. Has there been any study done about this? Also, what if it is a high temp (around 75 degrees C) for just 20 minutes? Or a low temperature for a long period of time? Thanks in advance for your input. -- Patrick Laurie HT(ASCP)QIHC plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Feb 5 11:31:46 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Feb 5 11:31:54 2010 Subject: [Histonet] Temperature and Humidity Monitoring System In-Reply-To: <8839B08E3ED7364E8CBBD53882C984D5141029B6@MAILSRV01.midmichigan.net> Message-ID: VWR has thermometers that also measure humidity - these are traceable, that's what we use to record daily temps and humidity. We have them in each of the rooms in the lab. The catalog number is 62344-734 they cost about $30.00 each. You can have them recalibrated yearly if you want to but sometimes it's just cheaper to purchase new ones before the calibration expires. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Friday, February 05, 2010 10:20 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Temperature and Humidity Monitoring System Anyone recently price one of these for a small to medium lab? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjay30 <@t> yahoo.com Fri Feb 5 12:16:58 2010 From: tjay30 <@t> yahoo.com (Timothy Jay) Date: Fri Feb 5 12:17:02 2010 Subject: [Histonet] in office path labs Message-ID: <395920.686.qm@web34305.mail.mud.yahoo.com> I completely understand where everyone is coming from on this issue. I was not trying to solicit work as there have been numerous inquires on the listserve about setting up an in-office path lab. I am sorry I offended you. I will not get into an unnecessary and unprofessional debate in this forum but I will state that although the in-office path labs are not popular amongst the pathology profession they are here and will continue to proliferate until the government states otherwise. Having said that, I can honestly say that I will only work w/ physicians that are committed to quality and safety. The labs are carefully and thoughtfully planned in terms of plumbing, electrical, and venting. I hire only? experienced and licensed histotechs, pay a great wage, and the labs I build are typically much?larger than what most techs are used to working in, plus my labs are all either on the first or second floors of building and all have windows. They are not pod labs. Lastly, the equipment I purchase for these labs is of great quality from national vendors (e.g. Leica, Sakura, ThermoFisher, etc). In terms of pathologists I partner with local pathologists and patholgists from large reference labs (e.g. Caris, GI Pathology, etc). I'm sorry that there is a lot of animosity towards those building these labs and I'm sure it makes no difference to you but I take great pride in what I do and will not compromise quality or safety to earn a buck. Thank you for your time and I wish everone well. ? Tim From baza0013 <@t> umn.edu Fri Feb 5 12:54:01 2010 From: baza0013 <@t> umn.edu (baza0013@umn.edu) Date: Fri Feb 5 12:54:07 2010 Subject: [Histonet] Sperm Hy-liter use? Message-ID: Hi Histonetters, The lab I just joined uses a sperm hyliter kit for the detection for the quantitative count of sperm in samples... however they seem to be getting a lot of non specific binding/background. Are there any of you out there that have a specific method for the detection of human sperm via immunoflourescence? Kits, protocols, suggestions are welcome! Thanks! Adam baza0013@umn.edu From Norm.Burnham <@t> propath.com Fri Feb 5 13:01:21 2010 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Fri Feb 5 13:01:26 2010 Subject: [Histonet] in office path labs In-Reply-To: <395920.686.qm@web34305.mail.mud.yahoo.com> References: <395920.686.qm@web34305.mail.mud.yahoo.com> Message-ID: <82C7248978CB50469FD6BA68EBBEFE6702D775D9@exchange.propathlab.com> Tim, Interesting -- please enlighten us as to what the minimum acceptable accreditation requirement you have established for your lab facilities? CAP, CLIA, COLA, or are you taking advantage of the in-office exemption from such standards? ____________________________________ Norm Burnham, MBA, MT(ASCP), CLS Director of Anatomic Laboratory Operations ProPath - The Leader in Pathology Services 8267 Elmbrook Drive, Suite 100 Dallas, TX 75247 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell Email: norm.burnham@propath.com To learn more about ProPath, please visit www.ProPath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timothy Jay Sent: Friday, February 05, 2010 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] in office path labs I completely understand where everyone is coming from on this issue. I was not trying to solicit work as there have been numerous inquires on the listserve about setting up an in-office path lab. I am sorry I offended you. I will not get into an unnecessary and unprofessional debate in this forum but I will state that although the in-office path labs are not popular amongst the pathology profession they are here and will continue to proliferate until the government states otherwise. Having said that, I can honestly say that I will only work w/ physicians that are committed to quality and safety. The labs are carefully and thoughtfully planned in terms of plumbing, electrical, and venting. I hire only? experienced and licensed histotechs, pay a great wage, and the labs I build are typically much?larger than what most techs are used to working in, plus my labs are all either on the first or second floors of building and all have windows. They are not pod labs. Lastly, the equipment I purchase for these labs is of great quality from national vendors (e.g. Leica, Sakura, ThermoFisher, etc). In terms of pathologists I partner with local pathologists and patholgists from large reference labs (e.g. Caris, GI Pathology, etc). I'm sorry that there is a lot of animosity towards those building these labs and I'm sure it makes no difference to you but I take great pride in what I do and will not compromise quality or safety to earn a buck. Thank you for your time and I wish everone well. ? Tim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Fri Feb 5 13:09:45 2010 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Feb 5 13:08:05 2010 Subject: [Histonet] GI, Uro, or Derm Path Lab Set Up In-Reply-To: <90354A475B420441B2A0396E5008D49692BEFF@copc-sbs.COPC.local> References: <657193.86909.qm@web34305.mail.mud.yahoo.com> <4B6AE7B7.7400.0077.1@harthosp.org> <90354A475B420441B2A0396E5008D49692BEFF@copc-sbs.COPC.local> Message-ID: <002201caa696$c7e26a50$0f01a8c0@biopath.local> And just remember to those techs out there accepting jobs like these because they are offering salaries that are way above the salary range, that the investors will want their money back at some point. And, if and when the pod lab is suffering financially, there go the investors, then the business and there goes your job. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Thursday, February 04, 2010 4:02 PM To: Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up I am in total agreement with both Richard and Felton here. Sorry Timothy if you are reading this but to me the bottom line is patient care. And in the end set-ups like this hurt patients. These "labs" and I'm using the term loosely are put in place to line the pockets of a small number of people at the top of a pyramid. I guess someone will always work to put things like this together if there is a demand. But please realize, if this is what you're involved in, the reality is... ~ Questionable facilities - Cramming professional, OSHA legal and ergonomically correct lab operations into small office spaces is a poor idea at best. Proper plumbing, ventilation and lab space are serious considerations and fall by the wayside when these operations are put together. ~ Qualified personnel - All of us posting on this list understand the fact that qualified, competent Histology Lab personnel are in demand and difficult to find. The wage for qualified, competent technical expertise does not fit into the plans of the originators of these operations. ~ Proper equipment and instruments - While the aim of these operations is limited in scope, basic functional equipment and instruments are required. In the overall scheme of things, pathology is still a bargain. However, money must be spent to properly equip a competent working lab with modern instruments. I could go on but that makes the basic point. And Timothy, lest you think that I'm shooting from the hip I assure I am not. Like most people on this list, I am a serious, diligent professional. I have interviewed people from "pod" labs that have worked under horrible conditions. I am also aware of a "pod" lab trying to get off the ground that shouldn't have an ice cube's chance in hell. The plans for this lab do not have proper plumbing, ventilation or space and there is no equipment. While a position has been advertised I cannot think of a single tech, worth his or her salt, that would consider associating themselves with such an ill-conceived venture. One last point - don't forget Timothy, that one day you may be that patient. Or it may be your mother, sister, daughter, wife, son, friend...I'll stop there. Every block, every slide, every piece of tissue that I or any of my staff encounter is a precious patient...a human life. So trying to dumb everything down, cut corners and make a small number of people wealthy in the process is an irresponsible and utterly ridiculous risk. The professionals in our field have worked hard for a good number of years to come out of the basement spaces and after-thought little nooks and crannies and other poor facilities to do the highly skilled work this profession demands. I for one will never abide going backwards and I don't believe people of good conscience and humanity will either. Sincerely yours, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Thursday, February 04, 2010 12:57 PM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu; Timothy Jay Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Especially when these physician hire unqualified people to run these labs and flood the histonet with their uneducated questions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 04, 2010 2:29 PM To: histonet@lists.utsouthwestern.edu; Timothy Jay Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are "Cherry-picking" the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Timothy Jay 2/4/2010 1:28 PM >>> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tjay30@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. Timothy Garcia-Jay, MHA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From baustin <@t> cbgbiotech.com Fri Feb 5 13:24:43 2010 From: baustin <@t> cbgbiotech.com (Beth Austin) Date: Fri Feb 5 13:31:29 2010 Subject: [Histonet] Formula 83 Message-ID: Mr. Richmond, I was contacted off site and asked to clarify something you posted to the Histonet. I hope you do not mind. The flashpoint of Formula 83 is not 7 degrees Fahrenheit. It is 7 degrees Celsius, or 45 degrees Fahrenheit. Also please note that the auto ignite temperature is 482 degrees Fahrenheit. Respectfully, Beth Austin-Sell CBG Biotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: GI, Uro, or Derm Path Lab Set Up (Sally Price) 2. Formula 83 (Robert Richmond) 3. RE: cleaning cryostat (Ingles Claire ) 4. What slides do you use? (Scott Hendricksen) 5. New slide drier recommendations please (Scott Hendricksen) 6. Re: BioCare immunostainer (Pat Laurie) 7. Slide baking before IHC (Pat Laurie) 8. Re: RE: GI, Uro, or Derm Path Lab Set Up (Jay Lundgren) 9. Temperature and Humidity Monitoring System (Teri.Hallada@midmichigan.org) 10. RE: Slide baking before IHC (Morken, Tim) 11. RE: Temperature and Humidity Monitoring System (Liz Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Thu, 4 Feb 2010 19:48:23 -0500 From: Sally Price Subject: [Histonet] RE: GI, Uro, or Derm Path Lab Set Up To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 ...and another thing -- since when is it OK to post such blatant solicitations on the HistoNet? other vendors are chastised for this on a regular basis! Sally ------------------------------ Message: 2 Date: Thu, 4 Feb 2010 21:14:50 -0500 From: Robert Richmond Subject: [Histonet] Formula 83 To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Allison Hutton, HTL(ASCP)cm, Lead Tech Histology, Doylestown Hospital, Doylestown, Pennsylvania asks: >>For those of you who are using Formula 83, could you please contact me off line? We have completed our demo but still have a few questions.<< Formula 83, offered by CBG Biotech Ltd., is a napthenic (cycloalkane) hydrocarbon used as a xylene substitute. It differs in structure from the aliphatic hydrocarbons such as Richard Allan's Clear-rite 3. It's in use in a lab I'm working in at the moment. I have two reservations about it. The first is that, unlike the aliphatics, it has a definite odor, which could be objectionable in a confined space. The second reservation: Formula 83 has a flash point of only 7 degrees Fahrenheit, lower than xylene (78 degrees) and other aliphatics, some of which have flash points as high as 144 F. The fire hazard here is worth considering. Formula 83 has been discussed before on Histonet, and the posts are worth looking up. When purchasing a new aliphatic, there are several important considerations. Your purchasing people need to understand that they can't change the xylene substitute on you, just because something a bit cheaper comes along. If you recycle, every different xylene substitute has a different distillation routine. It's important to ask everyone in the lab - in particular, the cytotechnologist is likely to have very strong opinions about the solvents they use in their ascending sequence before coverslipping. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Thu, 4 Feb 2010 22:44:24 -0600 From: "Ingles Claire " Subject: RE: [Histonet] cleaning cryostat To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I work in a Mohs Lab. (high output frozen skin) We wipe out the cryostat with 95% ETOH and clear out the shavings every day we use our machines. We normally tear down and completely defrost our cryostats every other month or so, depending on humidity and frost build up. (and available time to have a cryostat down.) We are not inspected by CAP, but JCAHO and CLIA who are at least as stringent. Claire ________________________________ I would like this information also. This is the first year we are CAP so any help I can get would be appreciated. Thanks so much have a great Friday and Week end. Kathy Gorham H.T. ------------------------------ Message: 4 Date: Thu, 4 Feb 2010 23:24:06 -0700 From: Scott Hendricksen Subject: [Histonet] What slides do you use? To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi, I was wondering what microscope slides you use. For the last couple of years I have noticed that a lot of the different brand slides are dirty. We have used Erie brand slides for many years, and they are the best quality I can find. Now even a lot of the Erie slides are dirty and we end up throwing a few away. Any suggestions for good clean slides at a reasonable price? Thanks again ! Scott Hendricksen HT(ASCP) ------------------------------ Message: 5 Date: Thu, 4 Feb 2010 23:35:33 -0700 From: Scott Hendricksen Subject: [Histonet] New slide drier recommendations please To: Histonet@lists.utsouthwestern.edu Message-ID: <7A5A7B7C-59A0-4AA1-BDBA-09EAD2C11F75@sfcn.org> Content-Type: text/plain; charset=us-ascii Hi, Does anyone have a suggestion for a forced air slide drier? We were looking at possibly the TBS SD-II-120. We have already tried a slide oven but it takes too long for the slides to dry, it was lacking a fan to circulate the air. Thanks, Scott Hendricksen HT(ASCP) ------------------------------ Message: 6 Date: Thu, 4 Feb 2010 22:47:22 -0800 From: Pat Laurie Subject: Re: [Histonet] BioCare immunostainer To: "Paal, Edina E." Cc: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We have an intellipath. It is a great machine. They have several unique innovations which makes customizing the work much easier. It depends what you need from the machine, but this had many innovations which helped our IHC department. It is also a completely open system which is becoming more and more difficult to find nowdays. On Thu, Feb 4, 2010 at 7:54 AM, Paal, Edina E. wrote: > We are at the verge of replacing our old immunostainer. We saw an > excellent introduction to BioCare but I would like to hear from real > people using it. Anything that makes you happy or unhappy? All > information would be appreciated. > > > Edina Paal, M.D. > Pathologist > VA Medical Center, Washington DC > 50 Irving St, N.W., Rm GB205 > Washington, DC 20422 > > Tel: (202) 518-4619 > Fax: (202) 745-8284 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com ------------------------------ Message: 7 Date: Fri, 5 Feb 2010 00:12:43 -0800 From: Pat Laurie Subject: [Histonet] Slide baking before IHC To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Histonet, I have heard rather anecdotally that if you bake slides at high temps (75 degrees to 80 degrees) before IHC for a long period of time (several hours to days), you may affect antigenicity for some antibodies. Has there been any study done about this? Also, what if it is a high temp (around 75 degrees C) for just 20 minutes? Or a low temperature for a long period of time? Thanks in advance for your input. -- Patrick Laurie HT(ASCP)QIHC plaurie@cellnetix.com ------------------------------ Message: 8 Date: Fri, 5 Feb 2010 06:59:24 -0500 From: Jay Lundgren Subject: Re: [Histonet] RE: GI, Uro, or Derm Path Lab Set Up To: Sally Price Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 These pod labs are a curse and make it that much harder for HTs and HTLs to get good paying, respected positions. Hospitals and reference labs are losing their biopsy workload to GI, GU, and Derm practices. Thomas is right. Some of these consultants leave copies of their credentials on the wall, and the practice then trains a histo monkey to turn out the work for $10./hr. When an inspection rolls around, oops, the HT is "on vacation", and the histo monkey goes back to sweeping the floors or filing, or whatever they were doing before, for the duration of the inspection. Pod labs are based on pure greed, and are part of the reason for spiralling health care costs in the U.S. Any histopathology technician or technologist that works for these consultants should realize that they are cutting their own throat in the long run. I notice that Timothy lists a Masters of Health Administration, but NOT an HT or HTL. Obviously he is familiar with health care economics, but that's not the whole picture. Money should never be the primary motivation in the practice of medicine. Sincerely, Jay A. Lundgren MS, HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Fri, 5 Feb 2010 12:20:01 -0500 From: Teri.Hallada@midmichigan.org Subject: [Histonet] Temperature and Humidity Monitoring System To: histonet@pathology.swmed.edu Message-ID: <8839B08E3ED7364E8CBBD53882C984D5141029B6@MAILSRV01.midmichigan.net> Content-Type: text/plain; charset=us-ascii Anyone recently price one of these for a small to medium lab? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. ------------------------------ Message: 10 Date: Fri, 5 Feb 2010 09:23:34 -0800 From: "Morken, Tim" Subject: RE: [Histonet] Slide baking before IHC To: "Pat Laurie" , "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4C0121DF474F@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Here is the reference: Effect of Slide Drying at 80Deg C on Immunohistochemistry A.F. Henwood J Histotechnology, VOl. 28, no. 1, March 2005 If you are an NSH member you can email the NSH office and ask for a pdf reprint. The author compares heating the slides at 80C for seven hours to one hour at 65C. Some antigens were adversely affected, some were not. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Friday, February 05, 2010 12:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide baking before IHC Histonet, I have heard rather anecdotally that if you bake slides at high temps (75 degrees to 80 degrees) before IHC for a long period of time (several hours to days), you may affect antigenicity for some antibodies. Has there been any study done about this? Also, what if it is a high temp (around 75 degrees C) for just 20 minutes? Or a low temperature for a long period of time? Thanks in advance for your input. -- Patrick Laurie HT(ASCP)QIHC plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 5 Feb 2010 10:31:46 -0700 From: "Liz Chlipala" Subject: RE: [Histonet] Temperature and Humidity Monitoring System To: , Message-ID: Content-Type: text/plain; charset="us-ascii" VWR has thermometers that also measure humidity - these are traceable, that's what we use to record daily temps and humidity. We have them in each of the rooms in the lab. The catalog number is 62344-734 they cost about $30.00 each. You can have them recalibrated yearly if you want to but sometimes it's just cheaper to purchase new ones before the calibration expires. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Friday, February 05, 2010 10:20 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Temperature and Humidity Monitoring System Anyone recently price one of these for a small to medium lab? Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 8 *************************************** From lblazek <@t> digestivespecialists.com Fri Feb 5 14:32:37 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Feb 5 14:32:59 2010 Subject: [Histonet] GI, Uro, or Derm Path Lab Set Up In-Reply-To: <90354A475B420441B2A0396E5008D49692BEFF@copc-sbs.COPC.local> References: <657193.86909.qm@web34305.mail.mud.yahoo.com> <4B6AE7B7.7400.0077.1@harthosp.org> <90354A475B420441B2A0396E5008D49692BEFF@copc-sbs.COPC.local> Message-ID: <5A2BD13465E061429D6455C8D6B40E390E976D9155@IBMB7Exchange.digestivespecialists.com> Dear all that are blatantly lumping all private or office specific GI, GU or Derm labs into one lump. It is very uneducated and juvenile to vent in such an unprofessional manner. You are not very well informed and/or ticked off at losing revenue from your hospital facility. Our facility complies with and exceeds all standards set by CAP, CLIA, OSHA and COLA. We are inspected at the required intervals by both COLA and CLIA. We do the required air quality validations. All of the staff is certified and participates in annual continuing education. Our main goal is patient care. Our pathologist interact with our clinicians on a regular basis. Our Quality Improvement, Quality Assessment and Quality Management is excellent. We have received the Laboratory Excellence Award from COLA and high praise by CLIA. All of our equipment is state of the art and regular maintenance is performed. There isn't a member of our team that would not either themselves or refer a member of their family to our facility. I don't think there is an employee here that wants to leave here and go back to the hospital facility and have to deal with the ever increasing drive to put out more and more work in less and less time. Our goal here is quality not quantity. This lab may not reflect all private labs but the ones I'm familiar with live by the same standards that we do. Do I sign my name or not..... oh what the heck.... Linda and staff! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Thursday, February 04, 2010 7:02 PM To: Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up I am in total agreement with both Richard and Felton here. Sorry Timothy if you are reading this but to me the bottom line is patient care. And in the end set-ups like this hurt patients. These "labs" and I'm using the term loosely are put in place to line the pockets of a small number of people at the top of a pyramid. I guess someone will always work to put things like this together if there is a demand. But please realize, if this is what you're involved in, the reality is... ~ Questionable facilities - Cramming professional, OSHA legal and ergonomically correct lab operations into small office spaces is a poor idea at best. Proper plumbing, ventilation and lab space are serious considerations and fall by the wayside when these operations are put together. ~ Qualified personnel - All of us posting on this list understand the fact that qualified, competent Histology Lab personnel are in demand and difficult to find. The wage for qualified, competent technical expertise does not fit into the plans of the originators of these operations. ~ Proper equipment and instruments - While the aim of these operations is limited in scope, basic functional equipment and instruments are required. In the overall scheme of things, pathology is still a bargain. However, money must be spent to properly equip a competent working lab with modern instruments. I could go on but that makes the basic point. And Timothy, lest you think that I'm shooting from the hip I assure I am not. Like most people on this list, I am a serious, diligent professional. I have interviewed people from "pod" labs that have worked under horrible conditions. I am also aware of a "pod" lab trying to get off the ground that shouldn't have an ice cube's chance in hell. The plans for this lab do not have proper plumbing, ventilation or space and there is no equipment. While a position has been advertised I cannot think of a single tech, worth his or her salt, that would consider associating themselves with such an ill-conceived venture. One last point - don't forget Timothy, that one day you may be that patient. Or it may be your mother, sister, daughter, wife, son, friend...I'll stop there. Every block, every slide, every piece of tissue that I or any of my staff encounter is a precious patient...a human life. So trying to dumb everything down, cut corners and make a small number of people wealthy in the process is an irresponsible and utterly ridiculous risk. The professionals in our field have worked hard for a good number of years to come out of the basement spaces and after-thought little nooks and crannies and other poor facilities to do the highly skilled work this profession demands. I for one will never abide going backwards and I don't believe people of good conscience and humanity will either. Sincerely yours, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Thursday, February 04, 2010 12:57 PM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu; Timothy Jay Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Especially when these physician hire unqualified people to run these labs and flood the histonet with their uneducated questions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 04, 2010 2:29 PM To: histonet@lists.utsouthwestern.edu; Timothy Jay Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are "Cherry-picking" the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Timothy Jay 2/4/2010 1:28 PM >>> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tjay30@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. Timothy Garcia-Jay, MHA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Fri Feb 5 15:14:49 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Feb 5 15:15:12 2010 Subject: [Histonet] GI, Uro, or Derm Path Lab Set Up In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390E976D9155@IBMB7Exchange.digestivespecialists.com> References: <657193.86909.qm@web34305.mail.mud.yahoo.com> <4B6AE7B7.7400.0077.1@harthosp.org> <90354A475B420441B2A0396E5008D49692BEFF@copc-sbs.COPC.local> <5A2BD13465E061429D6455C8D6B40E390E976D9155@IBMB7Exchange.digestivespecialists.com> Message-ID: Since you have a pathologist on staff that qualifies you as a reference lab and you are required to meet the same standards as a hospital. So really they were not talking about you Linda -----Original Message----- From: Blazek, Linda [mailto:lblazek@digestivespecialists.com] Sent: Friday, February 05, 2010 2:33 PM To: 'Thomas Jasper'; Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Dear all that are blatantly lumping all private or office specific GI, GU or Derm labs into one lump. It is very uneducated and juvenile to vent in such an unprofessional manner. You are not very well informed and/or ticked off at losing revenue from your hospital facility. Our facility complies with and exceeds all standards set by CAP, CLIA, OSHA and COLA. We are inspected at the required intervals by both COLA and CLIA. We do the required air quality validations. All of the staff is certified and participates in annual continuing education. Our main goal is patient care. Our pathologist interact with our clinicians on a regular basis. Our Quality Improvement, Quality Assessment and Quality Management is excellent. We have received the Laboratory Excellence Award from COLA and high praise by CLIA. All of our equipment is state of the art and regular maintenance is performed. There isn't a member of our team that would not either themselves or refer a member of their family to our facility. I don't think there is an employee here that wants to leave here and go back to the hospital facility and have to deal with the ever increasing drive to put out more and more work in less and less time. Our goal here is quality not quantity. This lab may not reflect all private labs but the ones I'm familiar with live by the same standards that we do. Do I sign my name or not..... oh what the heck.... Linda and staff! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Thursday, February 04, 2010 7:02 PM To: Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up I am in total agreement with both Richard and Felton here. Sorry Timothy if you are reading this but to me the bottom line is patient care. And in the end set-ups like this hurt patients. These "labs" and I'm using the term loosely are put in place to line the pockets of a small number of people at the top of a pyramid. I guess someone will always work to put things like this together if there is a demand. But please realize, if this is what you're involved in, the reality is... ~ Questionable facilities - Cramming professional, OSHA legal and ergonomically correct lab operations into small office spaces is a poor idea at best. Proper plumbing, ventilation and lab space are serious considerations and fall by the wayside when these operations are put together. ~ Qualified personnel - All of us posting on this list understand the fact that qualified, competent Histology Lab personnel are in demand and difficult to find. The wage for qualified, competent technical expertise does not fit into the plans of the originators of these operations. ~ Proper equipment and instruments - While the aim of these operations is limited in scope, basic functional equipment and instruments are required. In the overall scheme of things, pathology is still a bargain. However, money must be spent to properly equip a competent working lab with modern instruments. I could go on but that makes the basic point. And Timothy, lest you think that I'm shooting from the hip I assure I am not. Like most people on this list, I am a serious, diligent professional. I have interviewed people from "pod" labs that have worked under horrible conditions. I am also aware of a "pod" lab trying to get off the ground that shouldn't have an ice cube's chance in hell. The plans for this lab do not have proper plumbing, ventilation or space and there is no equipment. While a position has been advertised I cannot think of a single tech, worth his or her salt, that would consider associating themselves with such an ill-conceived venture. One last point - don't forget Timothy, that one day you may be that patient. Or it may be your mother, sister, daughter, wife, son, friend...I'll stop there. Every block, every slide, every piece of tissue that I or any of my staff encounter is a precious patient...a human life. So trying to dumb everything down, cut corners and make a small number of people wealthy in the process is an irresponsible and utterly ridiculous risk. The professionals in our field have worked hard for a good number of years to come out of the basement spaces and after-thought little nooks and crannies and other poor facilities to do the highly skilled work this profession demands. I for one will never abide going backwards and I don't believe people of good conscience and humanity will either. Sincerely yours, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Thursday, February 04, 2010 12:57 PM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu; Timothy Jay Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Especially when these physician hire unqualified people to run these labs and flood the histonet with their uneducated questions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 04, 2010 2:29 PM To: histonet@lists.utsouthwestern.edu; Timothy Jay Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are "Cherry-picking" the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Timothy Jay 2/4/2010 1:28 PM >>> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tjay30@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. Timothy Garcia-Jay, MHA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From tjasper <@t> copc.net Fri Feb 5 15:40:33 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Feb 5 15:40:37 2010 Subject: FW: [Histonet] GI, Uro, or Derm Path Lab Set Up Message-ID: <90354A475B420441B2A0396E5008D496731AF0@copc-sbs.COPC.local> For all. tj -----Original Message----- From: Thomas Jasper Sent: Friday, February 05, 2010 1:34 PM To: 'Blazek, Linda' Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Dear Linda, I have received private responses similar to yours. Seeing that you've posted for everyone I am compelled to respond. Let me start out by saying my issue, complaint, concern - whatever you want to call it obviously is not aimed at an operation such as yours. As a matter of fact my service is a stand-alone Histo/Cyto practice. We are full service, accredited and located 2 blocks from our main med center. Our situation is like a path department in a hospital, minus the politics, etc. I'm saying this because I am not against, private, above board, accredited operations, which are run ethically and free of ulterior motives. I apologize if anyone took offense, but I think it should be clear if your not what I've described, then your not it. Secondly, it was not my intention to vent in an uneducated, juvenile and unprofessional manner. Quite the opposite as a matter of fact. Here's what I know about so called "pod" labs, which by the way does not lump everyone into one group. I interviewed a candidate 2 years ago that came from a "pod" lab. I was appalled as she described the working conditions, facilities, lack of equipment and support she received with this service. Improper ventilation and plumbing, inadequate space, under-qualified assistance, unreal expectations from those in charge. This was an unethical practice at best and I'm at a loss to understand how it was allowed to legally operate. Through professional contacts I've been made aware of more and more "fly-by-night" ventures which are based on a lucrative financial reward for a few at the top. This is at the expense of facility and technical support. Along with this comes patient care risk. Nine months ago I lost an excellent tech to a "pod" lab. Within 2 weeks he was calling and e-mailing back, regretful of ever getting involved with this operation. He was lied to, overworked, underpaid and totally mis-lead by a poorly conceived and financially unbalanced venture. Fortunately, he will be rejoining us next week and he basically cannot get away from these people quickly enough. Now in our area plans for a "pod" lab are in the works. This facility does not have the space, ventilation, plumbing or staff. The group behind this venture has a history of upsetting behavior in the medical community. Let's just say they're controversial at times. As I mentioned previously they've advertised for a tech but I have no idea how or what they think they're going to pull off. I'm sorry but there are a ton of red flags here. The only reason I can think of that these operations are allowed to exist is that they skirt regulations somehow. And this has been alluded to by others posting on this list. My point is that I personally cannot abide subpar operations, that exist only to line the pockets of unscrupulous parties. Which in turn potentially leaves patients and well meaning staff in it's wake. If this is not you, great, more power to you and carry on. If this is what you're involved in consider yourself put on notice and remember karma can be a real bitch. Hopefully clear and not misunderstood, Thomas Jasper -----Original Message----- From: Blazek, Linda [mailto:lblazek@digestivespecialists.com] Sent: Friday, February 05, 2010 12:33 PM To: Thomas Jasper; Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Dear all that are blatantly lumping all private or office specific GI, GU or Derm labs into one lump. It is very uneducated and juvenile to vent in such an unprofessional manner. You are not very well informed and/or ticked off at losing revenue from your hospital facility. Our facility complies with and exceeds all standards set by CAP, CLIA, OSHA and COLA. We are inspected at the required intervals by both COLA and CLIA. We do the required air quality validations. All of the staff is certified and participates in annual continuing education. Our main goal is patient care. Our pathologist interact with our clinicians on a regular basis. Our Quality Improvement, Quality Assessment and Quality Management is excellent. We have received the Laboratory Excellence Award from COLA and high praise by CLIA. All of our equipment is state of the art and regular maintenance is performed. There isn't a member of our team that would not either themselves or refer a member of their family to our facility. I don't think there is an employee here that wants to leave here and go back to the hospital facility and have to deal with the ever increasing drive to put out more and more work in less and less time. Our goal here is quality not quantity. This lab may not reflect all private labs but the ones I'm familiar with live by the same standards that we do. Do I sign my name or not..... oh what the heck.... Linda and staff! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Thursday, February 04, 2010 7:02 PM To: Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up I am in total agreement with both Richard and Felton here. Sorry Timothy if you are reading this but to me the bottom line is patient care. And in the end set-ups like this hurt patients. These "labs" and I'm using the term loosely are put in place to line the pockets of a small number of people at the top of a pyramid. I guess someone will always work to put things like this together if there is a demand. But please realize, if this is what you're involved in, the reality is... ~ Questionable facilities - Cramming professional, OSHA legal and ergonomically correct lab operations into small office spaces is a poor idea at best. Proper plumbing, ventilation and lab space are serious considerations and fall by the wayside when these operations are put together. ~ Qualified personnel - All of us posting on this list understand the fact that qualified, competent Histology Lab personnel are in demand and difficult to find. The wage for qualified, competent technical expertise does not fit into the plans of the originators of these operations. ~ Proper equipment and instruments - While the aim of these operations is limited in scope, basic functional equipment and instruments are required. In the overall scheme of things, pathology is still a bargain. However, money must be spent to properly equip a competent working lab with modern instruments. I could go on but that makes the basic point. And Timothy, lest you think that I'm shooting from the hip I assure I am not. Like most people on this list, I am a serious, diligent professional. I have interviewed people from "pod" labs that have worked under horrible conditions. I am also aware of a "pod" lab trying to get off the ground that shouldn't have an ice cube's chance in hell. The plans for this lab do not have proper plumbing, ventilation or space and there is no equipment. While a position has been advertised I cannot think of a single tech, worth his or her salt, that would consider associating themselves with such an ill-conceived venture. One last point - don't forget Timothy, that one day you may be that patient. Or it may be your mother, sister, daughter, wife, son, friend...I'll stop there. Every block, every slide, every piece of tissue that I or any of my staff encounter is a precious patient...a human life. So trying to dumb everything down, cut corners and make a small number of people wealthy in the process is an irresponsible and utterly ridiculous risk. The professionals in our field have worked hard for a good number of years to come out of the basement spaces and after-thought little nooks and crannies and other poor facilities to do the highly skilled work this profession demands. I for one will never abide going backwards and I don't believe people of good conscience and humanity will either. Sincerely yours, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Thursday, February 04, 2010 12:57 PM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu; Timothy Jay Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Especially when these physician hire unqualified people to run these labs and flood the histonet with their uneducated questions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 04, 2010 2:29 PM To: histonet@lists.utsouthwestern.edu; Timothy Jay Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are "Cherry-picking" the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Timothy Jay 2/4/2010 1:28 PM >>> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tjay30@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. Timothy Garcia-Jay, MHA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kimberly.Marshall <@t> ahss.org Fri Feb 5 15:41:05 2010 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Fri Feb 5 15:41:17 2010 Subject: [Histonet] Radioactive tissue Message-ID: Hello out there Getting ready for our first CAP soon and notice they question the handling of tissue that may contain radioactive material. I am going to talk to the x-ray folks but wanted some input from the Histo-net. Such as how long you need to keep it in the lead lined container? Can histo add formalin before putting it in the container? Thanks in advance for your input. And thanks so much for all the folks that helped me with my Wright's Giemsa issue. It's a work on progress but feel I will get there to the "Perfect" color someday. Have a great weekend. Kimberly ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From BoozerKA <@t> ah.org Fri Feb 5 15:42:45 2010 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Fri Feb 5 15:43:04 2010 Subject: [Histonet] Billing code for kidney core adequacy Message-ID: <4B6C2054.4AA8.00C0.0@ah.org> Could someone please clarify the correct billing CPT codes for a pathologist who looks at a kidney biopsy for adequacy before being sent off to referral for EM, IF and LM. We were using 88172 but are now being told to use 88333 and 88334. Thank you, Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org From Wanda.Smith <@t> HCAhealthcare.com Fri Feb 5 15:47:46 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Fri Feb 5 15:47:51 2010 Subject: [Histonet] Billing code for kidney core adequacy In-Reply-To: <4B6C2054.4AA8.00C0.0@ah.org> References: <4B6C2054.4AA8.00C0.0@ah.org> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1384C57D28@NADCWPMSGCMS03.hca.corpad.net> We use 88329 Pathology consultation during surgery. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, February 05, 2010 4:43 PM To: Histonet Subject: [Histonet] Billing code for kidney core adequacy Could someone please clarify the correct billing CPT codes for a pathologist who looks at a kidney biopsy for adequacy before being sent off to referral for EM, IF and LM. We were using 88172 but are now being told to use 88333 and 88334. Thank you, Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anonwums1 <@t> gmail.com Fri Feb 5 16:38:05 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Fri Feb 5 16:38:14 2010 Subject: [Histonet] Alternatives to BioQuant for Bone Histomorphometry Message-ID: <858249121002051438h85948f4h896f6676fad94f3d@mail.gmail.com> Hi all, I am looking for an alternative program to BioQuant for bone histomorphometry. We need to quantify the number of osteoblasts / osteoclasts per bone surface area as well as the percent surface area occupied by those cells. We have a computer with BioQuant on it available, but we find the software to be incredibly clunky and often nearly impossible to use. Based on my limited attempts to use it, it very well might rank as one of the worst user interfaces I've ever seen, and I was trained in computer science and have seen my fair share of horrible software (I'm looking at you, Lotus Notes). Anyhow, any suggestions on a (preferably cheap / free) replacement for doing simple analysis or how to make BioQuant less painful would be very helpful. Thanks, Adam From mpence <@t> grhs.net Fri Feb 5 16:47:31 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Feb 5 16:47:38 2010 Subject: [Histonet] GI, Uro, or Derm Path Lab Set Up In-Reply-To: <90354A475B420441B2A0396E5008D496731AF0@copc-sbs.COPC.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D3E@is-e2k3.grhs.net> 'consider yourself put on notice and remember karma can be a real bitch.' Thomas - was this really called for! I think not. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Friday, February 05, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] GI, Uro, or Derm Path Lab Set Up For all. tj -----Original Message----- From: Thomas Jasper Sent: Friday, February 05, 2010 1:34 PM To: 'Blazek, Linda' Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Dear Linda, I have received private responses similar to yours. Seeing that you've posted for everyone I am compelled to respond. Let me start out by saying my issue, complaint, concern - whatever you want to call it obviously is not aimed at an operation such as yours. As a matter of fact my service is a stand-alone Histo/Cyto practice. We are full service, accredited and located 2 blocks from our main med center. Our situation is like a path department in a hospital, minus the politics, etc. I'm saying this because I am not against, private, above board, accredited operations, which are run ethically and free of ulterior motives. I apologize if anyone took offense, but I think it should be clear if your not what I've described, then your not it. Secondly, it was not my intention to vent in an uneducated, juvenile and unprofessional manner. Quite the opposite as a matter of fact. Here's what I know about so called "pod" labs, which by the way does not lump everyone into one group. I interviewed a candidate 2 years ago that came from a "pod" lab. I was appalled as she described the working conditions, facilities, lack of equipment and support she received with this service. Improper ventilation and plumbing, inadequate space, under-qualified assistance, unreal expectations from those in charge. This was an unethical practice at best and I'm at a loss to understand how it was allowed to legally operate. Through professional contacts I've been made aware of more and more "fly-by-night" ventures which are based on a lucrative financial reward for a few at the top. This is at the expense of facility and technical support. Along with this comes patient care risk. Nine months ago I lost an excellent tech to a "pod" lab. Within 2 weeks he was calling and e-mailing back, regretful of ever getting involved with this operation. He was lied to, overworked, underpaid and totally mis-lead by a poorly conceived and financially unbalanced venture. Fortunately, he will be rejoining us next week and he basically cannot get away from these people quickly enough. Now in our area plans for a "pod" lab are in the works. This facility does not have the space, ventilation, plumbing or staff. The group behind this venture has a history of upsetting behavior in the medical community. Let's just say they're controversial at times. As I mentioned previously they've advertised for a tech but I have no idea how or what they think they're going to pull off. I'm sorry but there are a ton of red flags here. The only reason I can think of that these operations are allowed to exist is that they skirt regulations somehow. And this has been alluded to by others posting on this list. My point is that I personally cannot abide subpar operations, that exist only to line the pockets of unscrupulous parties. Which in turn potentially leaves patients and well meaning staff in it's wake. If this is not you, great, more power to you and carry on. If this is what you're involved in consider yourself put on notice and remember karma can be a real bitch. Hopefully clear and not misunderstood, Thomas Jasper -----Original Message----- From: Blazek, Linda [mailto:lblazek@digestivespecialists.com] Sent: Friday, February 05, 2010 12:33 PM To: Thomas Jasper; Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Dear all that are blatantly lumping all private or office specific GI, GU or Derm labs into one lump. It is very uneducated and juvenile to vent in such an unprofessional manner. You are not very well informed and/or ticked off at losing revenue from your hospital facility. Our facility complies with and exceeds all standards set by CAP, CLIA, OSHA and COLA. We are inspected at the required intervals by both COLA and CLIA. We do the required air quality validations. All of the staff is certified and participates in annual continuing education. Our main goal is patient care. Our pathologist interact with our clinicians on a regular basis. Our Quality Improvement, Quality Assessment and Quality Management is excellent. We have received the Laboratory Excellence Award from COLA and high praise by CLIA. All of our equipment is state of the art and regular maintenance is performed. There isn't a member of our team that would not either themselves or refer a member of their family to our facility. I don't think there is an employee here that wants to leave here and go back to the hospital facility and have to deal with the ever increasing drive to put out more and more work in less and less time. Our goal here is quality not quantity. This lab may not reflect all private labs but the ones I'm familiar with live by the same standards that we do. Do I sign my name or not..... oh what the heck.... Linda and staff! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Thursday, February 04, 2010 7:02 PM To: Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up I am in total agreement with both Richard and Felton here. Sorry Timothy if you are reading this but to me the bottom line is patient care. And in the end set-ups like this hurt patients. These "labs" and I'm using the term loosely are put in place to line the pockets of a small number of people at the top of a pyramid. I guess someone will always work to put things like this together if there is a demand. But please realize, if this is what you're involved in, the reality is... ~ Questionable facilities - Cramming professional, OSHA legal and ergonomically correct lab operations into small office spaces is a poor idea at best. Proper plumbing, ventilation and lab space are serious considerations and fall by the wayside when these operations are put together. ~ Qualified personnel - All of us posting on this list understand the fact that qualified, competent Histology Lab personnel are in demand and difficult to find. The wage for qualified, competent technical expertise does not fit into the plans of the originators of these operations. ~ Proper equipment and instruments - While the aim of these operations is limited in scope, basic functional equipment and instruments are required. In the overall scheme of things, pathology is still a bargain. However, money must be spent to properly equip a competent working lab with modern instruments. I could go on but that makes the basic point. And Timothy, lest you think that I'm shooting from the hip I assure I am not. Like most people on this list, I am a serious, diligent professional. I have interviewed people from "pod" labs that have worked under horrible conditions. I am also aware of a "pod" lab trying to get off the ground that shouldn't have an ice cube's chance in hell. The plans for this lab do not have proper plumbing, ventilation or space and there is no equipment. While a position has been advertised I cannot think of a single tech, worth his or her salt, that would consider associating themselves with such an ill-conceived venture. One last point - don't forget Timothy, that one day you may be that patient. Or it may be your mother, sister, daughter, wife, son, friend...I'll stop there. Every block, every slide, every piece of tissue that I or any of my staff encounter is a precious patient...a human life. So trying to dumb everything down, cut corners and make a small number of people wealthy in the process is an irresponsible and utterly ridiculous risk. The professionals in our field have worked hard for a good number of years to come out of the basement spaces and after-thought little nooks and crannies and other poor facilities to do the highly skilled work this profession demands. I for one will never abide going backwards and I don't believe people of good conscience and humanity will either. Sincerely yours, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Thursday, February 04, 2010 12:57 PM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu; Timothy Jay Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Especially when these physician hire unqualified people to run these labs and flood the histonet with their uneducated questions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 04, 2010 2:29 PM To: histonet@lists.utsouthwestern.edu; Timothy Jay Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are "Cherry-picking" the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Timothy Jay 2/4/2010 1:28 PM >>> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tjay30@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. Timothy Garcia-Jay, MHA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jeff.Rinker <@t> providence.org Fri Feb 5 18:09:43 2010 From: Jeff.Rinker <@t> providence.org (Rinker, Jeff A.) Date: Fri Feb 5 18:13:40 2010 Subject: [Histonet] Microwave processors Message-ID: I am looking for information on the sakura microwave processor. We are considering getting one, but before that I would like to hear some opinion on the system(good or bad). I would also like to hear how it effected the work flow . Thank you Jeff Lead HT (ASCP) Sacred Heart Med Center From tahseen <@t> brain.net.pk Sat Feb 6 03:38:38 2010 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Sat Feb 6 03:38:46 2010 Subject: [Histonet] Frozen Sections Slides per Day Question In-Reply-To: <552462.75622.qm@web112516.mail.gq1.yahoo.com> References: <552462.75622.qm@web112516.mail.gq1.yahoo.com> Message-ID: <27023.202.125.145.178.1265449118.squirrel@brain.net.pk> Dear Soofia Sissiqui, 25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units code # 5019 entire day ( 8 hours 480 minte) Muhammad Tahseen Histology Supervisor SKMTH&RC Lahore Pakistan > Hi dear histology experts, > I will greatly appreciate if somebody can let me know the estimated number > of slides (with 3 sections per slide) per day on average a lab technician > can cut (of frozen tissue). > ? > I am a lab technician?( not a histotech)?and?work alone in?a highly > complex testing specialzed low volume dermatology lab.My job > description?includes 30 % of immunohistochemistry related dutites. ?I cut > skin frozen sections and do immunohistochemistry? manually for several > years?. My routiene panel?consists of?12?antibodies for ?T-cells??surface > markers. Ocassionally??I?add another panel of 8 antibodies for B-Cells. > I am very slow in cutting sections and strugle a lot to get good sections. > If I spend entire day (?8 hours)?just ?cutting? 3-4 section on each slide > with slow speed. What? number of?slides should be considered as efficent > cutting? > What number of blocks (12 slides for each block with 3-4 sections) should > I finish in one day? > ? > Help me please if you can. Thanks. Soofia > ? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Barry.R.Rittman <@t> uth.tmc.edu Sat Feb 6 07:03:35 2010 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Sat Feb 6 07:03:42 2010 Subject: [Histonet] Frozen Sections Slides per Day Question In-Reply-To: <27023.202.125.145.178.1265449118.squirrel@brain.net.pk> References: <552462.75622.qm@web112516.mail.gq1.yahoo.com>, <27023.202.125.145.178.1265449118.squirrel@brain.net.pk> Message-ID: <75A0543E23D3A7458012D9E02EDBEC00098CEE30F8@UTHCMS1.uthouston.edu> While I no longer cut frozen sections, I did so for several years. The number of blocks and sections you cut depends on so many factors including the type of tissue sectioned, changing knife and roll plate positions, time to seal and store blocks after cutting, your expertise etc. So that, depending on circumstances, the number can vary considerably from lab to lab. What mostly concerned me about your email was that you would be doing this all day. There are so many injuries that can be caused by repetitive tasks in histology, most commonly cutting of frozen and paraffin wax sections. Apart from this is the potential for errors that occur with continuous repetitive tasks and increases with the length of time period in which these are done.. I would recommend that multitasking with some other tasks alternating with the frozen sections Just my opinion. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tahseen@brain.net.pk [tahseen@brain.net.pk] Sent: Saturday, February 06, 2010 3:38 AM To: soofia siddiqui Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen Sections Slides per Day Question Dear Soofia Sissiqui, 25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units code # 5019 entire day ( 8 hours 480 minte) Muhammad Tahseen Histology Supervisor SKMTH&RC Lahore Pakistan > Hi dear histology experts, > I will greatly appreciate if somebody can let me know the estimated number > of slides (with 3 sections per slide) per day on average a lab technician > can cut (of frozen tissue). > > I am a lab technician ( not a histotech) and work alone in a highly > complex testing specialzed low volume dermatology lab.My job > description includes 30 % of immunohistochemistry related dutites. I cut > skin frozen sections and do immunohistochemistry manually for several > years . My routiene panel consists of 12 antibodies for T-cells surface > markers. Ocassionally I add another panel of 8 antibodies for B-Cells. > I am very slow in cutting sections and strugle a lot to get good sections. > If I spend entire day ( 8 hours) just cutting 3-4 section on each slide > with slow speed. What number of slides should be considered as efficent > cutting? > What number of blocks (12 slides for each block with 3-4 sections) should > I finish in one day? > > Help me please if you can. Thanks. Soofia > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Feb 6 09:16:09 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 6 09:16:14 2010 Subject: [Histonet] Microwave processors In-Reply-To: Message-ID: <14262.66735.qm@web65711.mail.ac4.yahoo.com> Under separate cover I am sending some information answering your answers. Ren? J. --- On Fri, 2/5/10, Rinker, Jeff A. wrote: From: Rinker, Jeff A. Subject: [Histonet] Microwave processors To: Histonet@lists.utsouthwestern.edu Date: Friday, February 5, 2010, 7:09 PM I am looking for information on the sakura microwave processor. We are considering getting one, but before that I would like to hear some opinion on the system(good or bad). I would also like to hear how it effected the work flow . Thank you Jeff Lead HT (ASCP) Sacred Heart Med Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Feb 6 09:19:02 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 6 09:19:08 2010 Subject: [Histonet] Frozen Sections Slides per Day Question In-Reply-To: <75A0543E23D3A7458012D9E02EDBEC00098CEE30F8@UTHCMS1.uthouston.edu> Message-ID: <783841.82216.qm@web65710.mail.ac4.yahoo.com> FS standard is 15 minutes/case. Ren? J. --- On Sat, 2/6/10, Rittman, Barry R wrote: From: Rittman, Barry R Subject: RE: [Histonet] Frozen Sections Slides per Day Question To: "tahseen@brain.net.pk" , "soofia siddiqui" Cc: "histonet@lists.utsouthwestern.edu" Date: Saturday, February 6, 2010, 8:03 AM While I no longer cut frozen sections, I did so for several years. The number of blocks and sections you cut depends on so many factors including the type of tissue sectioned,? changing knife and roll plate positions, time to seal and store blocks after cutting, your expertise? etc. So that, depending on circumstances, the number can vary considerably from lab to lab. What mostly concerned me about your email was that you would be doing this all day. There are so many injuries that can be caused by repetitive tasks in histology, most commonly cutting of frozen and paraffin wax sections. Apart from this is the potential for errors that occur with continuous repetitive tasks and increases with the length of time period in which these are done.. I would recommend that multitasking with some other tasks alternating with the frozen sections Just my opinion. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tahseen@brain.net.pk [tahseen@brain.net.pk] Sent: Saturday, February 06, 2010 3:38 AM To: soofia siddiqui Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen Sections Slides per Day Question Dear Soofia Sissiqui, 25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units code # 5019 entire day ( 8 hours 480 minte) Muhammad Tahseen Histology Supervisor SKMTH&RC Lahore Pakistan > Hi dear histology experts, > I will greatly appreciate if somebody can let me know the estimated number > of slides (with 3 sections per slide) per day on average a lab technician > can cut (of frozen tissue). > > I am a lab technician ( not a histotech) and work alone in a highly > complex testing specialzed low volume dermatology lab.My job > description includes 30 % of immunohistochemistry related dutites.? I cut > skin frozen sections and do immunohistochemistry? manually for several > years . My routiene panel consists of 12 antibodies for? T-cells? surface > markers. Ocassionally? I add another panel of 8 antibodies for B-Cells. > I am very slow in cutting sections and strugle a lot to get good sections. > If I spend entire day ( 8 hours) just? cutting? 3-4 section on each slide > with slow speed. What? number of slides should be considered as efficent > cutting? > What number of blocks (12 slides for each block with 3-4 sections) should > I finish in one day? > > Help me please if you can. Thanks. Soofia > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiegowan <@t> msn.com Sat Feb 6 10:20:34 2010 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Sat Feb 6 10:20:40 2010 Subject: [Histonet] Microwave processors In-Reply-To: References: Message-ID: Please include me on this as well. I have done quite a bit of research but am interested in what anyone has to say. Thanks, Christie > Date: Fri, 5 Feb 2010 16:09:43 -0800 > From: Jeff.Rinker@providence.org > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Microwave processors > > I am looking for information on the sakura microwave processor. We are considering getting one, but before that I would like to hear some opinion on the system(good or bad). I would also like to hear how it effected the work flow . > Thank you > Jeff Lead HT (ASCP) > Sacred Heart Med Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Sat Feb 6 11:41:52 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sat Feb 6 11:42:01 2010 Subject: [Histonet] Alternatives to BioQuant for Bone Histomorphometry In-Reply-To: <858249121002051438h85948f4h896f6676fad94f3d@mail.gmail.com> Message-ID: Adam I'm not sure if there is anything free out there that will do what you want to do. You might want to try Image J or NIH Image those are free. We perform this type of analysis in Image Pro, but that software is going to run you about $4000.00. Also you have to know how to "script" or program code to create a custom code that will work for your application. I think that image pro is written in visual basic, so if you know visual basic you could program your own code. Since you have a computer science background you might be able to do this, possibly even with the BioQuant software. You need to see if you can get a library from them. This will be lists of code, each will perform a specific task, so you can essentially "script" custom code. That's how we approach custom analysis here. Custom code development can take some time, depending upon how much QC/QA you want to perform to make sure that your code is capturing the data correctly. Plus I would not say that the type of analysis you are attempting to perform is simple. I'm going to forward your e-mail to our image analysis guy to see what he has to say, he is more of an expert than I am on programming code since he is the one who does it for us. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam . Sent: Friday, February 05, 2010 3:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alternatives to BioQuant for Bone Histomorphometry Hi all, I am looking for an alternative program to BioQuant for bone histomorphometry. We need to quantify the number of osteoblasts / osteoclasts per bone surface area as well as the percent surface area occupied by those cells. We have a computer with BioQuant on it available, but we find the software to be incredibly clunky and often nearly impossible to use. Based on my limited attempts to use it, it very well might rank as one of the worst user interfaces I've ever seen, and I was trained in computer science and have seen my fair share of horrible software (I'm looking at you, Lotus Notes). Anyhow, any suggestions on a (preferably cheap / free) replacement for doing simple analysis or how to make BioQuant less painful would be very helpful. Thanks, Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michaelbackhus <@t> aol.com Sun Feb 7 08:58:47 2010 From: michaelbackhus <@t> aol.com (Mike) Date: Sun Feb 7 08:59:03 2010 Subject: [Histonet] We use the microwave processor and we love it we have about 5 . Works great fir small tissue not large. The only issue is when it breaks it has to be sent to the shop they send you a loaner but you are with out one for a few days so you must have a back up. Mike ht Message-ID: <161C3F48-48A1-4173-A950-EC6B9EA50591@aol.com> Sent from my iPhone From pruegg <@t> ihctech.net Sun Feb 7 09:55:22 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Feb 7 09:56:07 2010 Subject: [Histonet] Slide baking before IHC In-Reply-To: <1AAF670737F193429070841C6B2ADD4C0121DF474F@EXMBMCB15.ucsfmedicalcenter.org> References: <1AAF670737F193429070841C6B2ADD4C0121DF474F@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <7B30AD8C22FB469EB4BF70989584020F@prueggihctechlt> I concur with Tim, this paper is a good reference for slide drying temps, as with everything else there is no rule that works for every antigen, I play it safe and try very hard to air dry draining until there is no water left on or under the section, and then I bake at temps not above 60dc for most things, but when it comes to bone and cartilage, I drain and air dry them and then lay them flat on heat plate at 45 dc overnight when possible. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, February 05, 2010 10:24 AM To: Pat Laurie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide baking before IHC Here is the reference: Effect of Slide Drying at 80Deg C on Immunohistochemistry A.F. Henwood J Histotechnology, VOl. 28, no. 1, March 2005 If you are an NSH member you can email the NSH office and ask for a pdf reprint. The author compares heating the slides at 80C for seven hours to one hour at 65C. Some antigens were adversely affected, some were not. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Friday, February 05, 2010 12:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide baking before IHC Histonet, I have heard rather anecdotally that if you bake slides at high temps (75 degrees to 80 degrees) before IHC for a long period of time (several hours to days), you may affect antigenicity for some antibodies. Has there been any study done about this? Also, what if it is a high temp (around 75 degrees C) for just 20 minutes? Or a low temperature for a long period of time? Thanks in advance for your input. -- Patrick Laurie HT(ASCP)QIHC plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Feb 7 10:01:51 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Feb 7 10:02:35 2010 Subject: [Histonet] GI, Uro, or Derm Path Lab Set Up In-Reply-To: References: <657193.86909.qm@web34305.mail.mud.yahoo.com><4B6AE7B7.7400.0077.1@harthosp.org> Message-ID: <5F4DE71EC794490C9A8681680BAD31D0@prueggihctechlt> The one's who flood histonet with questions are better than the ones running these labs that we never hear from because they don't even know what histology is much less that histonet exists. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Thursday, February 04, 2010 1:57 PM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu; Timothy Jay Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Especially when these physician hire unqualified people to run these labs and flood the histonet with their uneducated questions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 04, 2010 2:29 PM To: histonet@lists.utsouthwestern.edu; Timothy Jay Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are "Cherry-picking" the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Timothy Jay 2/4/2010 1:28 PM >>> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tjay30@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. Timothy Garcia-Jay, MHA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Feb 7 10:15:56 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Feb 7 10:16:35 2010 Subject: SPAM-LOW: Re: [Histonet] autofluorescence experiment In-Reply-To: References: Message-ID: <4F42B29E0417484ABE444429B029E20B@prueggihctechlt> In my experience the auto fluorescence from formalin fixation is green seen with the fitc filter so when I am doing IF on formalin fixed tissues I try to use a different colormetric label such as Texas Red, that way even though the bone and red cells are autofluoresing it won't be seen with my TR filter. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Thursday, February 04, 2010 10:26 AM To: Histonet@lists.utsouthwestern.edu; Nicole Collette Subject: SPAM-LOW: Re: [Histonet] autofluorescence experiment I don't know any argument against using both copper sulphate and Sudan black B, and you have shown that the combination reduces the autofluorescence of bone. It's interesting that both are applied after immunofluorescent staining and are reported to cause some reduction of the desired fluorescence (Schnell et al 1999 J. Histochem. Cytochem. 47:719-730; Baschong et al 2001 J. Histochem. Cytochem. 449:1565-1571). Earlier chemical methods for suppressing autofluorescence involved pre-treatment of sections with either a heavy metal compound or a non-fluorescent dye. 0.2% osmium tetroxide for 5 mins is very effective; needs hours in slowly running tap water to wash it out before fluorescent staining. The dye Direct blue 1 (CI 24410), also known as Chicago sky blue B, Niagara blue 6B and pontamine sky blue, was recommended by Cowen et al 1985 Histochemistry 82:205-208 and Kutvolgyi et al 2006 Biotech. Histochem. 81:4-6. Cowen et al used a 0.05% solution of the dye in PBS with 1% DMSO. I haven't treid it myself. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicole Collette Date: Wednesday, February 3, 2010 19:12 Subject: [Histonet] autofluorescence experiment To: Histonet@lists.utsouthwestern.edu > Hello, All, > > I am working on doing some IF stains with bone samples (lucky > me!). I > am having a difficult time sometimes to assess the antibody > since the > autofluorescence gets in the way. I am using undecalcified, FFPE > sections (late embryo and neonate mouse bones). Without > treatment, I > see autofluorescence everywhere, but most frustrating is the red > blood cells and the mineralized matrix. It took me a while to > get > samples that are properly fixed, section well, and stay on the > slides, so I am not particularly jazzed about messing with the > fixation protocol. Thus, I conducted an experiment today of > several > published and voodoo methods to reduce autofluorescence with > samples > that did NOT undergo the IF protocol : > > no treatment > photobleaching, fluorescent light box, up to 2 weeks > photobleaching, UV crosslinking light, 2 inches from source, 24h > 10mM copper sulfate in 50mM ammonium acetate, pH 5.0 > 0.25% (v/v) NH3 in 70% ethanol (in water) > 0.25% (v/v) NH4OH in 70% ethanol (in water) (since it was > unclear > from the published reference what the source of ammonia was, and > I > have made this mistake before on some other thing) > 0.3% (w/v) Sudan Black in 70% ethanol (in water) > > I found that the most effective treatment in my hands is Sudan > Black > for cell-based autofluorescence, but it did not seem to impact > the > autofluorescence from the mineralized matrix at all, while > copper > sulfate had significant impact on the autofluorescence in > mineralized > bone, but did not quench cell-based autofluorescence as well as > the > Sudan Black (it had an even but incomplete impact over the > entire > section). I have tried Sudan Black on my slides before, and have > found that it did not seem to interfere significantly with the > antibody signal. I modified the Sudan Black protocol to > eliminate the > "goopies" and "chunkies" resulting on my slides from previous > attempts, and am happy with the results- a light, even stain. > > My question to all you chemistry folks: Is there some reason why > copper sulfate treatment followed by washing and subsequent > Sudan > Black treatment (and more washing) cannot or should not be used? > I > tried them together on my unstained slides, they look pretty > darn > fabulous. Just striving for clean data and beautiful pictures. > > Thanks again for all your help, > Sincerely, > > Nicole Collette > Lawrence Livermore National Lab/ UC Berkeley > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Feb 7 10:24:35 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Feb 7 10:25:15 2010 Subject: [Histonet] TRAP assay - acid phosphatase In-Reply-To: References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23FDC@PHSXMB30.partners.org> Message-ID: <8B857727EA5340B1B93C5F4B9076D568@prueggihctechlt> I use TRAP enzyme histochemical assay from Sigma for osteoclasts on formalin fixed EDTA decaled Bone paraffin sections, like Liz mentioned as well as undecalcified frozen bone sections cut with a permanent tungsten carbide blade in the cryostat using the Instrumedics tape transfer system. My frozen bone sections are also formalin fixed and sucrose infiltrated before freezing. I do not fix the frozen sections because they are already fixed in formalin. One thing I do with that assay that may not be mentioned in the protocol which is mainly for smears, is that I incubate at 37dc for at least an hour and sometimes for 4 hours or more on paraffin or frozen tape sections. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, February 03, 2010 3:49 PM To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] TRAP assay - acid phosphatase We have used it on FFPE EDTA decaled mouse bone, with a little bit of fiddling with the protocol it works pretty good Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sherwood, Margaret Sent: Wed 2/3/2010 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TRAP assay - acid phosphatase Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on mouse tibia for IHC. If so, 1) can you do it on paraffin and/or frozen sections? 2) if you do it on frozens, do you decalcify first? 3) what type of blade do you use for sectioning on the cryostat? 3) what procedure do you follow after sectioning: do you fix slides (i.e. acetone, etc.) Thank you. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Feb 7 10:48:46 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Feb 7 10:49:27 2010 Subject: SPAM-LOW: [Histonet] Re : KP Markers In-Reply-To: <738912.31896.qm@web30206.mail.mud.yahoo.com> References: <738912.31896.qm@web30206.mail.mud.yahoo.com> Message-ID: <4F268DA564C74653A288BEC184389340@prueggihctechlt> Yea we just got ours but they were on BO for a few months. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Gutierrez Sent: Friday, January 29, 2010 7:42 AM To: Histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re : KP Markers KP?Marker pens are sold?by Mercedes Medical. I use them and I really like them.?The?box I ordered is still on back order so you want to order as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Feb 7 11:01:15 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Feb 7 11:01:56 2010 Subject: SPAM-LOW: [Histonet] Re: Histonet Digest, Vol 74, Issue 34 In-Reply-To: <828544.67370.qm@web23205.mail.ird.yahoo.com> References: <828544.67370.qm@web23205.mail.ird.yahoo.com> Message-ID: What I do is make a cell block. If you can get the spheroids into suspension in a test tube, you could fix in formalin, spin them down in a centrifuge and then take of the supernatant, add warmed histogel and resuspend in the histogel, spin it down to get your spheroid in the bottom of the tube, then let the histogel cool so it will harden, you can then tease the hardened plug out of the tube, wrap it in paper and process into paraffin, keep track of the bottom where your cells should be and embed them down, they should be on the surface of your paraffin tissue block for sectioning. Another way is if you can see the spheroids, after fixing, move them with fine forceps, you could fill an embedding mold with warm liquid histogel, then place the spheroid in the mold, cool the histogel til it hardens, then pop it out of the mold and wrap in paper and process into paraffin as the cell block above. We get HISTOGEL from Richard Allan. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rhonda Henshall-Powell Sent: Thursday, January 28, 2010 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: Histonet Digest, Vol 74, Issue 34 Dear Nick, When I was growing and harvesting 3D cell cultures (spheroids grown in matrigel) we would remove the culture medium, draw up the gel in a needle-less syringe and place in to OCT embedding medium (Tissue Tek) before freezing in Liquid Nitrogen. I would then cut 5-10um sections and perform IHC or immunofluorescence. Hope this helps - but it looks like you have a lot of good suggestions already. Best Regards, Rhonda Rhonda Henshall-Powell, Ph.D. > > ------------------------------ > > Message: 2 > Date: Wed, 27 Jan 2010 10:25:42 -0800 (PST) > From: Nicholas David Evans > > Dear all, > > I was hoping someone might be able to offer me some advice > on embedding and sectioning cell cultures. > > In short we are growing cells which form 3D dome-like > structures on tissue culture plastic. Does anyone have any > experience or advice to offer on embedding the cultures in > situ before sectioning? I have seen various methods in the > literature, which often use Epon to embed the material > followed by sawing away the plastic, but if anyone can offer > some tips on other possible (easier) ways of doing it, or > can refer me to some useful literature, I'd be very > grateful. > > I would like to have simple 10um sections at 90 degrees to > the substrate, which I can use for IHC. > > Best wishes > Nick > > > > ------------------------------ > > Message: 3 > Date: Wed, 27 Jan 2010 15:19:36 -0500 > From: Geoff McAuliffe > > Hi Nick: > > You can use the Epon substitutes such as EmBed 812. Fix, > osmicate, > dehydrate as usual, but omit the proplylene oxide as it > will react with > the plastic dish. Epon substitutes will mix with ethanol. I > used 2:1 > then 1:1 then 1:2 ratios of absolute ethanol to "Epon" with > catalyst > added with agitation. Then several changes of pure Epon and > polymerize. > Yes, you will have to saw away the plastic, if you try to > section the > Epon-plastic combo the two will separate. > How much easier do you want it to be? > > Geoff > ------------------------------ > > Message: 5 > Date: Wed, 27 Jan 2010 15:39:05 -0500 > From: "Sherwood, Margaret " > Subject: RE: [HISTONET] embedding cell cultures > Nick, > > I am assuming that your 3D cells only grow on > plastic.? They make plastic cover > slips which, if your cells attach to them and grow, might > make the embedding > much easier.? You would follow the same method as > stated, but then you could > invert the coverslips on a beem capsule and separate the > coverslip from capsule > with liquid nitrogen.? However, I have never done it > with plastic coverslips > (only glass), so not sure if they would easily separate > from capsule with liquid > nitrogen. If anyone else has done so, please weigh in. > > Peggy??? > > ------------------------------ > Message: 6 > Date: Wed, 27 Jan 2010 15:44:37 -0500 > From: Peggy Bisher > Subject: Re: [HISTONET] embedding cell cultures > > I have done just what you are talking about using Aclar. It > is a plastic > embedding film (purchased from EMS). It works great for > us. > > Margaret E. Bisher > Electron Microscopy & Histology Core Facility Manager > Department of Molecular Biology > Princeton University > Moffett Laboratory, Room 113 > Princeton, New Jersey > Office: (609) 258-7026 > Fax: (609) 258-8468 > mbisher@princeton.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From napoli <@t> siscom.net Sun Feb 7 13:09:20 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Sun Feb 7 13:09:27 2010 Subject: [Histonet] out-of-hospital environment labs Message-ID: <4b6f0fe0.36b.6960.378380979@siscom.net> Here are the plain facts for anyone whose paycheck they are defending inside hospital-based environments: All labs in the USA are owned by some kind of PRIVATE interest. Get used to it. For all those histology and lab "EXPERTS" out there who think they know what they are talking about regarding out-of-hospital labs, PLEASE, FOR PITY SAKE, enlighten all of us "UNTRAINED" histo professionals as to how the MBA and Venture capital $ organizations that run hospitals and PROFIT off of Technical Components are any different from anyone else who wants to build or own a lab? You are really only repeating the lamentations of the privately contracted Pathology group entities and the Hospital MBA $ PEOPLE who go ahead and divide up the "spoils" you are so quick to blame board-certified clinicians (some of whom are fellowed in their respective fields) for claiming. The real truth of the matter is that until you REALLY know what you are talking about, you just seem ignorant. I have shown these posts to a number of pathologists and dermatologists and they LAUGHED! Here's what you should do to validate your points criticizing out-of-hospital labs: (please do this, because I think you have the brass) 1) go to your pathology group (the docs) and ask to see a complete breakdown of how they are paid vs how much work is coming in the door. Many are privately contracted groups. 2) pay a visit to your hospital administration department and ask them to show you their "books" and how much they profit from lab tests (including, but not necessarily limited to the technical component on histo tests; or ask them about their financial arrangements with the CORPORATE lab they have in house that pays most histotech checks and contracts with the physician group. 3) learn a little more about the differences between many (not all) general pathologists and clinician dermatopathologists.n Sounds like some of you "haven't a clue." MOST OF ALL: why not act a little more maturely. Some of you are very mature in your comments, some are just petty. I will debate anyone on this. Please fire away. I have DEEP resources to counter anything you say. I will also not hesitate to start lamenting the problems with hospital labs. They aren't God's gift to patient care across the board either. I DO have the persepective because I have worked in hospitals (military and civilian)and have also worked the out-of-hospital lab world. Thank you to all of you who are engaged in "building-up" our excellent community of histology professionals. For those of you who want to beat people up and look a fool, then please find your way somewhere else. This is a professional forum, not your own bully pulpit. SO...next time someone wants to "paint with a broad brushstroke" and demean or belittle jr techs out there or cast dipersions on highly-qualified medical professionals, please remember that you, too, may be living in a glass house. Regards, Andrew Burgeson Histotechnologist From napoli <@t> siscom.net Sun Feb 7 13:32:04 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Sun Feb 7 13:32:08 2010 Subject: [Histonet] Clarification on "private labs" Message-ID: <4b6f1534.2e7.71e2.239166332@siscom.net> When referring to all labs in the USA being "privately-owned," I am, of course, excluding government facilities. BUT...even those facilities employ people who make $ working in this field and so have some interest in the discussions. Also, due to the fact that MEDICARE is such a big factor in US medical reimbursements, anyone with a Medicare ID who gets paid by the government is, in a sense, a "government" provider. So in this sense, the system is mixed. My post refers specifically to non-government labs. (with the understanding that most everyone bills medicare) AB From ccpath <@t> gmail.com Sun Feb 7 14:04:55 2010 From: ccpath <@t> gmail.com (j k) Date: Sun Feb 7 14:05:00 2010 Subject: [Histonet] out-of-hospital environment labs In-Reply-To: <4b6f0fe0.36b.6960.378380979@siscom.net> References: <4b6f0fe0.36b.6960.378380979@siscom.net> Message-ID: It's called 'o-v-e-r-u-t-i-l-i-z-a-t-i-o-n'.... http://www.ascp.org/pdf/Advocacy/Self-Referral-Policy-Statement-09.aspx "Studies by the U.S. Department of Health and Human Services (HHS) and other government agencies have shown that referrals to entities in which physicians have a financial relationship encourage excessive use of services." "The OIG audits reveal an alarming increase in the utilization of anatomic pathology services once these group practices were able to capture the pathology-related revenues." jim On 2/7/10, Andrew Burgeson wrote: > > > For all those histology and lab "EXPERTS" out there who > think they know what they are talking about regarding > out-of-hospital labs, PLEASE, FOR PITY SAKE, enlighten all > of us "UNTRAINED" histo professionals as to how the MBA and > Venture capital $ organizations that run hospitals and > PROFIT off of Technical Components are any different from > anyone else who wants to build or own a lab? From dlschneider <@t> gmail.com Sun Feb 7 15:03:39 2010 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Sun Feb 7 15:03:56 2010 Subject: [Histonet] Clarification on pod labs In-Reply-To: <4b6f1534.2e7.71e2.239166332@siscom.net> References: <4b6f1534.2e7.71e2.239166332@siscom.net> Message-ID: <9CB689AA-869E-44C8-8D69-51C6ED72F423@gmail.com> What we have here is market distortion by a payment scheme that doesn't reflect the real costs. You have to ask yourself "why would a urologist/dermatologist/gastroenterologist want to mess with a histology lab?" Follow the money. Processing biopsies, I.e. the technical component of CPT code 88305, is ridiculously profitable. It's reimbursement is way out of proportion to the actual costs involved, which is why these clinicians are willing to invest in small inefficient in-office labs -- they're still going to clean up. Cha Ching! Hospital based pathologists rely on the profitable biopsy business to make up for the time and resources devoted to less profitable sides of their work, for example resection specimens ( CPT code 88307's and 88309's)that are reimbursed more than a biopsy but not proportionate to the cost of processing them ( complex grossing requring more time and expertise from a PA or often a pathologist, as well as many blocks to process, embed, and cut, and more time at the scope reviewing these many slides.). When someone else cherry picks the biopsies, hospital labs suffer. But you say, it's just business, it's the American way. But it's only that way because of fat reimbursement for the TC on 88305. Cut that significantly, and all these in-office labs become liabilities, not profit centers. The urologists/derms/GI's will then close their labs, and their histotechs will get kicked to the curb. Given the healthcare climate in this country, and the fact that everybody knows the technical component on 88305 is relatively rich, how likely do you think that is? Dan Schneider (Obligatory Disclosure: I a Hospital Based Pathologist.) Sent from my iPhone On Feb 7, 2010, at 1:32 PM, "Andrew Burgeson" wrote: > When referring to all labs in the USA being > "privately-owned," I am, of course, excluding government > facilities. BUT...even those facilities employ people who > make $ working in this field and so have some interest in > the discussions. > > Also, due to the fact that MEDICARE is such a big factor in > US medical reimbursements, anyone with a Medicare ID who > gets paid by the government is, in a sense, a "government" > provider. So in this sense, the system is mixed. > > My post refers specifically to non-government labs. (with > the understanding that most everyone bills medicare) > > AB > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john <@t> imebinc.com Sun Feb 7 15:41:53 2010 From: john <@t> imebinc.com (John O'Brien) Date: Sun Feb 7 15:36:44 2010 Subject: [Histonet] RE: Histonet Digest, Vol 75, Issue 11 Message-ID: <001b01caa83e$5d4852a0$4a01a8c0@EXECUTIVE01> I may have a folder of old cartoon from year past. J -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. We use the microwave processor and we love it we have about 5 . Works great fir small tissue not large. The only issue is when it breaks it has to be sent to the shop they send you a loaner but you are with out one for a few days so you must have a back up. Mike ht (Mike) 2. RE: Slide baking before IHC (Patsy Ruegg) 3. RE: GI, Uro, or Derm Path Lab Set Up (Patsy Ruegg) 4. RE: SPAM-LOW: Re: [Histonet] autofluorescence experiment (Patsy Ruegg) 5. RE: TRAP assay - acid phosphatase (Patsy Ruegg) 6. RE: SPAM-LOW: [Histonet] Re : KP Markers (Patsy Ruegg) 7. RE: SPAM-LOW: [Histonet] Re: Histonet Digest, Vol 74, Issue 34 (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Sun, 7 Feb 2010 09:58:47 -0500 From: Mike Subject: [Histonet] We use the microwave processor and we love it we have about 5 . Works great fir small tissue not large. The only issue is when it breaks it has to be sent to the shop they send you a loaner but you are with out one for a few days so you must have a back up. Mike ht To: "Histonet@lists.utsouthwestern.edu" Message-ID: <161C3F48-48A1-4173-A950-EC6B9EA50591@aol.com> Content-Type: text/plain; charset=us-ascii; format=flowed Sent from my iPhone ------------------------------ Message: 2 Date: Sun, 7 Feb 2010 08:55:22 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] Slide baking before IHC To: "'Morken, Tim'" , "'Pat Laurie'" , Message-ID: <7B30AD8C22FB469EB4BF70989584020F@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" I concur with Tim, this paper is a good reference for slide drying temps, as with everything else there is no rule that works for every antigen, I play it safe and try very hard to air dry draining until there is no water left on or under the section, and then I bake at temps not above 60dc for most things, but when it comes to bone and cartilage, I drain and air dry them and then lay them flat on heat plate at 45 dc overnight when possible. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, February 05, 2010 10:24 AM To: Pat Laurie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide baking before IHC Here is the reference: Effect of Slide Drying at 80Deg C on Immunohistochemistry A.F. Henwood J Histotechnology, VOl. 28, no. 1, March 2005 If you are an NSH member you can email the NSH office and ask for a pdf reprint. The author compares heating the slides at 80C for seven hours to one hour at 65C. Some antigens were adversely affected, some were not. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Friday, February 05, 2010 12:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide baking before IHC Histonet, I have heard rather anecdotally that if you bake slides at high temps (75 degrees to 80 degrees) before IHC for a long period of time (several hours to days), you may affect antigenicity for some antibodies. Has there been any study done about this? Also, what if it is a high temp (around 75 degrees C) for just 20 minutes? Or a low temperature for a long period of time? Thanks in advance for your input. -- Patrick Laurie HT(ASCP)QIHC plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sun, 7 Feb 2010 09:01:51 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up To: "'Nails, Felton'" , "'Richard Cartun'" , , "'Timothy Jay'" Message-ID: <5F4DE71EC794490C9A8681680BAD31D0@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" The one's who flood histonet with questions are better than the ones running these labs that we never hear from because they don't even know what histology is much less that histonet exists. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Thursday, February 04, 2010 1:57 PM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu; Timothy Jay Subject: RE: [Histonet] GI, Uro, or Derm Path Lab Set Up Especially when these physician hire unqualified people to run these labs and flood the histonet with their uneducated questions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 04, 2010 2:29 PM To: histonet@lists.utsouthwestern.edu; Timothy Jay Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up We don't need anymore pathology laboratories. What we need is support of existing laboratories, especially hospital-based labs. GI and GU physicians are "Cherry-picking" the technical revenue that should be going to hospital labs. Let's reform health care; make it more efficient and less expensive. We don't need to be putting more money in clinicians' pockets. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Timothy Jay 2/4/2010 1:28 PM >>> For those needing help putting an in-office path lab together whether you are GI, Urology, or Derm please send me an email at tjay30@yahoo.com or call me at 775-830-1591. I have a consulting business that specializes in putting these labs together. References provided upon request. Timothy Garcia-Jay, MHA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ---- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================================================================== ==== == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sun, 7 Feb 2010 09:15:56 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: Re: [Histonet] autofluorescence experiment To: "'John Kiernan'" , , "'Nicole Collette'" Message-ID: <4F42B29E0417484ABE444429B029E20B@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" In my experience the auto fluorescence from formalin fixation is green seen with the fitc filter so when I am doing IF on formalin fixed tissues I try to use a different colormetric label such as Texas Red, that way even though the bone and red cells are autofluoresing it won't be seen with my TR filter. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Thursday, February 04, 2010 10:26 AM To: Histonet@lists.utsouthwestern.edu; Nicole Collette Subject: SPAM-LOW: Re: [Histonet] autofluorescence experiment I don't know any argument against using both copper sulphate and Sudan black B, and you have shown that the combination reduces the autofluorescence of bone. It's interesting that both are applied after immunofluorescent staining and are reported to cause some reduction of the desired fluorescence (Schnell et al 1999 J. Histochem. Cytochem. 47:719-730; Baschong et al 2001 J. Histochem. Cytochem. 449:1565-1571). Earlier chemical methods for suppressing autofluorescence involved pre-treatment of sections with either a heavy metal compound or a non-fluorescent dye. 0.2% osmium tetroxide for 5 mins is very effective; needs hours in slowly running tap water to wash it out before fluorescent staining. The dye Direct blue 1 (CI 24410), also known as Chicago sky blue B, Niagara blue 6B and pontamine sky blue, was recommended by Cowen et al 1985 Histochemistry 82:205-208 and Kutvolgyi et al 2006 Biotech. Histochem. 81:4-6. Cowen et al used a 0.05% solution of the dye in PBS with 1% DMSO. I haven't treid it myself. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicole Collette Date: Wednesday, February 3, 2010 19:12 Subject: [Histonet] autofluorescence experiment To: Histonet@lists.utsouthwestern.edu > Hello, All, > > I am working on doing some IF stains with bone samples (lucky > me!). I > am having a difficult time sometimes to assess the antibody > since the > autofluorescence gets in the way. I am using undecalcified, FFPE > sections (late embryo and neonate mouse bones). Without > treatment, I > see autofluorescence everywhere, but most frustrating is the red > blood cells and the mineralized matrix. It took me a while to > get > samples that are properly fixed, section well, and stay on the > slides, so I am not particularly jazzed about messing with the > fixation protocol. Thus, I conducted an experiment today of > several > published and voodoo methods to reduce autofluorescence with > samples > that did NOT undergo the IF protocol : > > no treatment > photobleaching, fluorescent light box, up to 2 weeks photobleaching, > UV crosslinking light, 2 inches from source, 24h 10mM copper sulfate > in 50mM ammonium acetate, pH 5.0 0.25% (v/v) NH3 in 70% ethanol (in > water) 0.25% (v/v) NH4OH in 70% ethanol (in water) (since it was > unclear > from the published reference what the source of ammonia was, and > I > have made this mistake before on some other thing) > 0.3% (w/v) Sudan Black in 70% ethanol (in water) > > I found that the most effective treatment in my hands is Sudan > Black > for cell-based autofluorescence, but it did not seem to impact > the > autofluorescence from the mineralized matrix at all, while > copper > sulfate had significant impact on the autofluorescence in > mineralized > bone, but did not quench cell-based autofluorescence as well as > the > Sudan Black (it had an even but incomplete impact over the > entire > section). I have tried Sudan Black on my slides before, and have > found that it did not seem to interfere significantly with the > antibody signal. I modified the Sudan Black protocol to > eliminate the > "goopies" and "chunkies" resulting on my slides from previous > attempts, and am happy with the results- a light, even stain. > > My question to all you chemistry folks: Is there some reason why > copper sulfate treatment followed by washing and subsequent > Sudan > Black treatment (and more washing) cannot or should not be used? > I > tried them together on my unstained slides, they look pretty > darn > fabulous. Just striving for clean data and beautiful pictures. > > Thanks again for all your help, > Sincerely, > > Nicole Collette > Lawrence Livermore National Lab/ UC Berkeley > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Sun, 7 Feb 2010 09:24:35 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] TRAP assay - acid phosphatase To: "'Liz Chlipala'" , "'Sherwood, Margaret '" , Message-ID: <8B857727EA5340B1B93C5F4B9076D568@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" I use TRAP enzyme histochemical assay from Sigma for osteoclasts on formalin fixed EDTA decaled Bone paraffin sections, like Liz mentioned as well as undecalcified frozen bone sections cut with a permanent tungsten carbide blade in the cryostat using the Instrumedics tape transfer system. My frozen bone sections are also formalin fixed and sucrose infiltrated before freezing. I do not fix the frozen sections because they are already fixed in formalin. One thing I do with that assay that may not be mentioned in the protocol which is mainly for smears, is that I incubate at 37dc for at least an hour and sometimes for 4 hours or more on paraffin or frozen tape sections. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, February 03, 2010 3:49 PM To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] TRAP assay - acid phosphatase We have used it on FFPE EDTA decaled mouse bone, with a little bit of fiddling with the protocol it works pretty good Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sherwood, Margaret Sent: Wed 2/3/2010 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TRAP assay - acid phosphatase Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on mouse tibia for IHC. If so, 1) can you do it on paraffin and/or frozen sections? 2) if you do it on frozens, do you decalcify first? 3) what type of blade do you use for sectioning on the cryostat? 3) what procedure do you follow after sectioning: do you fix slides (i.e. acetone, etc.) Thank you. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Sun, 7 Feb 2010 09:48:46 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] Re : KP Markers To: "'Vanessa Gutierrez'" , Message-ID: <4F268DA564C74653A288BEC184389340@prueggihctechlt> Content-Type: text/plain; charset="iso-8859-1" Yea we just got ours but they were on BO for a few months. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Gutierrez Sent: Friday, January 29, 2010 7:42 AM To: Histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re : KP Markers KP?Marker pens are sold?by Mercedes Medical. I use them and I really like them.?The?box I ordered is still on back order so you want to order as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Sun, 7 Feb 2010 10:01:15 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] Re: Histonet Digest, Vol 74, Issue 34 To: "'Rhonda Henshall-Powell'" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" What I do is make a cell block. If you can get the spheroids into suspension in a test tube, you could fix in formalin, spin them down in a centrifuge and then take of the supernatant, add warmed histogel and resuspend in the histogel, spin it down to get your spheroid in the bottom of the tube, then let the histogel cool so it will harden, you can then tease the hardened plug out of the tube, wrap it in paper and process into paraffin, keep track of the bottom where your cells should be and embed them down, they should be on the surface of your paraffin tissue block for sectioning. Another way is if you can see the spheroids, after fixing, move them with fine forceps, you could fill an embedding mold with warm liquid histogel, then place the spheroid in the mold, cool the histogel til it hardens, then pop it out of the mold and wrap in paper and process into paraffin as the cell block above. We get HISTOGEL from Richard Allan. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rhonda Henshall-Powell Sent: Thursday, January 28, 2010 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: Histonet Digest, Vol 74, Issue 34 Dear Nick, When I was growing and harvesting 3D cell cultures (spheroids grown in matrigel) we would remove the culture medium, draw up the gel in a needle-less syringe and place in to OCT embedding medium (Tissue Tek) before freezing in Liquid Nitrogen. I would then cut 5-10um sections and perform IHC or immunofluorescence. Hope this helps - but it looks like you have a lot of good suggestions already. Best Regards, Rhonda Rhonda Henshall-Powell, Ph.D. > > ------------------------------ > > Message: 2 > Date: Wed, 27 Jan 2010 10:25:42 -0800 (PST) > From: Nicholas David Evans > > Dear all, > > I was hoping someone might be able to offer me some advice > on embedding and sectioning cell cultures. > > In short we are growing cells which form 3D dome-like structures on > tissue culture plastic. Does anyone have any experience or advice to > offer on embedding the cultures in situ before sectioning? I have seen > various methods in the literature, which often use Epon to embed the > material followed by sawing away the plastic, but if anyone can offer > some tips on other possible (easier) ways of doing it, or > can refer me to some useful literature, I'd be very > grateful. > > I would like to have simple 10um sections at 90 degrees to the > substrate, which I can use for IHC. > > Best wishes > Nick > > > > ------------------------------ > > Message: 3 > Date: Wed, 27 Jan 2010 15:19:36 -0500 > From: Geoff McAuliffe > > Hi Nick: > > You can use the Epon substitutes such as EmBed 812. Fix, osmicate, > dehydrate as usual, but omit the proplylene oxide as it > will react with > the plastic dish. Epon substitutes will mix with ethanol. I > used 2:1 > then 1:1 then 1:2 ratios of absolute ethanol to "Epon" with > catalyst > added with agitation. Then several changes of pure Epon and > polymerize. > Yes, you will have to saw away the plastic, if you try to > section the > Epon-plastic combo the two will separate. > How much easier do you want it to be? > > Geoff > ------------------------------ > > Message: 5 > Date: Wed, 27 Jan 2010 15:39:05 -0500 > From: "Sherwood, Margaret " > Subject: RE: [HISTONET] embedding cell cultures > Nick, > > I am assuming that your 3D cells only grow on > plastic.? They make plastic cover > slips which, if your cells attach to them and grow, might make the > embedding much easier.? You would follow the same method as > stated, but then you could > invert the coverslips on a beem capsule and separate the > coverslip from capsule > with liquid nitrogen.? However, I have never done it > with plastic coverslips > (only glass), so not sure if they would easily separate > from capsule with liquid > nitrogen. If anyone else has done so, please weigh in. > > Peggy > > ------------------------------ > Message: 6 > Date: Wed, 27 Jan 2010 15:44:37 -0500 > From: Peggy Bisher > Subject: Re: [HISTONET] embedding cell cultures > > I have done just what you are talking about using Aclar. It is a > plastic embedding film (purchased from EMS). It works great for > us. > > Margaret E. Bisher > Electron Microscopy & Histology Core Facility Manager Department of > Molecular Biology Princeton University > Moffett Laboratory, Room 113 > Princeton, New Jersey > Office: (609) 258-7026 > Fax: (609) 258-8468 > mbisher@princeton.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 11 **************************************** From napoli <@t> siscom.net Mon Feb 8 00:43:22 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Mon Feb 8 00:43:28 2010 Subject: [Histonet] Out-of-hospital labs comment on OVERUTILIZATION of "hyphens" Message-ID: <4b6fb28a.205.44e2.1029737477@siscom.net> I think its pretty obvious that we are all talking about o-v-e-r-u-t-i-l-i-z-a-t-i-o-n, just as the "hyphen" is so overutilized in your post! Please tell me ALL about how dermatologists overutilize. What is the metric? How are GI and Urol and dermatology the same? I would like to show some dermatologists I know. There are other polarities to this debate... other "facets,"if you will. What do you have to say about "choice?" What do you have to say about clinico-pathologic correlations? Dermatologists are very concerned about who reads their labs. Very. If you do not believe me, then ask a few? Some hospital groups have dermatologist/dermatopathologists that are GREAT!!!!! But not all, unfortunately. (Some of the best ones I know of are in hospital settings...and get lots of derm work! World class dermpaths) Also, unlike GI, for example, IS THERE AN AVERAGE biopsy rate? One of the biggest problems with this whole line of discussion is that people are aggregating dermatology/dermatopathology with GI and Urology. THEY-ARE-ALL-DIFFERENT. I would like to point out that there are more named diseases in dermatology than any other system or organ....by far.50 pages of CPTs. One mole, one nail, one seb k? Should the dermatologist leave off areas of concern because people are going to say that they are overutilizing? Why havent the hospital groups complained to the clinicians that THEY serve when "too many" (whatever that means) specimens come in? (Hospital labs even do slide preparation for local clinician dermatopathologists sometimes.) What say you in that instance? There are ALL kinds of permutations to this stuff. It is not all biopsies, either...lots of surgicals. We could get into MOHS surgeons vs plastic surgeons and how in bed they are seen to be with hospital labs if you like, but that will take a lot more time. Maybe you should ask some of them why they choose to manage melanomas? Many dermatologists keep labs in house because they feel they can better diagnose and consequently serve the patient. Also, in case you are unaware, dermatology residencies are typically very heavy in histology, due to the fact that there is a significant pathology component on the clinical board test. This makes many clinical dermatologists quite savvy with dermpath. This is yet another distinction. Often clinical dermatopathologists who train clinician residents gain their loyalty and confidence, resulting in getting their work. Melanocytic and inflammatory skin cases are typically more difficult to diagnose, and so these groups often either hire a fellowed dermpath who can read that stuff, or they have to find someone willing to read hard cases and that can be difficult. I am confident that dermatologists are quite capable of deciding who they should send their lab work to and that they are capable of getting precision reports based on their needs and their decisions. I know because I have seen it and I have heard it time and time again. I have also seen many initial bx reports from general path labs that are totally misdiagnosed, and the patient's life potentially saved because the dermatologist and dermpath correctly diagnosed the lesion as MM. What about that? Next time a primary care physician wants to take a mole off you or your loved one, think twice and then think even harder about where that bx will be tested. I know where I would want it to go. Furthermore, there are many types of physicians out there (predominately some family practice and other primary care practitioners)other than dermatologists who improperly excise dangerous melanocytic proliferations, most often by not excising deeply enough, and causing the histologic results on re-excision to be difficult to interpret due to the base of the original biopsy site having too many lymphocytes present to classify the disease and measure its depth. If you care...as it seems you genuinely DO...I think you are a good person. I am sure you are ethical and try to do the very best for the patient and to protect the physicians (perhaps you are one)from unnecessary liability. Your comment is taken in and has validity, but I believe requires clarification. Many dermatologists I am certain would find that to be an insult and a gross misrepresentation of what they do. Best wishes kind sir. Regards, AB From jkiernan <@t> uwo.ca Mon Feb 8 01:39:20 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Feb 8 01:39:24 2010 Subject: [Histonet] We use the microwave processor and we love it we have about 5 . Works great fir small tissue not large. The only issue is when it breaks it has to be sent to the shop they send you a loaner but you are with out one for a few days so you must have a back up. Mike ht In-Reply-To: <161C3F48-48A1-4173-A950-EC6B9EA50591@aol.com> References: <161C3F48-48A1-4173-A950-EC6B9EA50591@aol.com> Message-ID: Next time, please put your message in the body of the email, instead of all in the Subject line? Thanks in advance, John Kiernan London, Canada - - - - - - ----- Original Message ----- From: Mike Date: Sunday, February 7, 2010 10:00 Subject: [Histonet] We use the microwave processor and we love it we have about 5 . Works great fir small tissue not large. The only issue is when it breaks it has to be sent to the shop they send you a loaner but you are with out one for a few days so you must have a back up. Mike ht To: "Histonet@lists.utsouthwestern.edu" > > > Sent from my iPhone > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kjones <@t> upei.ca Mon Feb 8 06:21:46 2010 From: Kjones <@t> upei.ca (Kathleen Jones) Date: Mon Feb 8 06:21:55 2010 Subject: [Histonet] Alternatives to BioQuant for Bone Histomorphometry In-Reply-To: <858249121002051438h85948f4h896f6676fad94f3d@mail.gmail.com> References: <858249121002051438h85948f4h896f6676fad94f3d@mail.gmail.com> Message-ID: <4B6FC99A0200008B0001E1C7@grpwise.novell.upei.ca> Hi Adam Have you checked out ImageJ? It's a free downloadable program that I have used for alveolar morphometry. Fairly user friendly, more so than BioQuant, although not quite as thorough. Good Luck Kathy Kathleen Jones Research Technician Pathology/Microbiology AVC - UPEI (902)566-0595 >>> "Adam ." 2/5/2010 6:38 PM >>> Hi all, I am looking for an alternative program to BioQuant for bone histomorphometry. We need to quantify the number of osteoblasts / osteoclasts per bone surface area as well as the percent surface area occupied by those cells. We have a computer with BioQuant on it available, but we find the software to be incredibly clunky and often nearly impossible to use. Based on my limited attempts to use it, it very well might rank as one of the worst user interfaces I've ever seen, and I was trained in computer science and have seen my fair share of horrible software (I'm looking at you, Lotus Notes). Anyhow, any suggestions on a (preferably cheap / free) replacement for doing simple analysis or how to make BioQuant less painful would be very helpful. Thanks, Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Mon Feb 8 06:45:05 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Feb 8 06:45:19 2010 Subject: [Histonet] Alternatives to BioQuant for Bone Histomorphometry In-Reply-To: <858249121002051438h85948f4h896f6676fad94f3d@mail.gmail.com> References: <858249121002051438h85948f4h896f6676fad94f3d@mail.gmail.com> Message-ID: Adam, What stain are you using for your quantitation and are you trying to perform measurements via the "thresholding" feature? Jack On Feb 5, 2010, at 4:38 PM, "Adam ." wrote: > Hi all, > > I am looking for an alternative program to BioQuant for bone > histomorphometry. We need to quantify the number of osteoblasts / > osteoclasts per bone surface area as well as the percent surface area > occupied by those cells. We have a computer with BioQuant on it > available, > but we find the software to be incredibly clunky and often nearly > impossible > to use. Based on my limited attempts to use it, it very well might > rank as > one of the worst user interfaces I've ever seen, and I was trained in > computer science and have seen my fair share of horrible software (I'm > looking at you, Lotus Notes). > > Anyhow, any suggestions on a (preferably cheap / free) replacement > for doing > simple analysis or how to make BioQuant less painful would be very > helpful. > > Thanks, > Adam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Vickroy.Jim <@t> mhsil.com Mon Feb 8 07:49:47 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Feb 8 07:49:57 2010 Subject: [Histonet] looking for microcassettes Message-ID: <24A4826E8EF0964D86BC5317306F58A54256F5A05E@mmc-mail.ad.mhsil.com> We have a Surgipath cassette printer and are currently using Surgipath cassettes. We are adding a microwave tissue processor and would like to use some biopsy cassettes, however some of the ones I've tried so far do not print on the printer. I do not think there is much adjustment possible in the printer so I need to find some cassettes that work in the printer. I am told Surgipath has a biopsy cassette and will try their's. Has anybody else found another cassette to work? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From anonwums1 <@t> gmail.com Mon Feb 8 08:09:09 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Mon Feb 8 08:09:17 2010 Subject: [Histonet] Alternatives to BioQuant for Bone Histomorphometry In-Reply-To: References: <858249121002051438h85948f4h896f6676fad94f3d@mail.gmail.com> Message-ID: <858249121002080609q1c236114qdf0566155b64e58c@mail.gmail.com> I am planning on using IHC using DAB stain to label the cells of interest (a subpopulation of bone cells) counterstained with hematoxylin. My coworker wants to do similar things with TRAP stained osteoclasts. The way I was trained how to do this would be turn off thresholding completely (the guy said it doesn't work well enough), outline the contours of the bone, and then outline the perimeter lined by the cells of interest, and finally click on each cell of interest. It seemed to me that doing this in BioQuant was needlessly complex and involved constantly loading and resetting these vectors each time, and if you forgot to do that, you could do a whole bunch of work and have BioQuant either compute the parameters completely wrong or simply discard what you did. Adam On Mon, Feb 8, 2010 at 6:45 AM, Jack Ratliff wrote: > Adam, > > What stain are you using for your quantitation and are you trying to > perform measurements via the "thresholding" feature? > > > Jack > > > On Feb 5, 2010, at 4:38 PM, "Adam ." wrote: > > Hi all, >> >> I am looking for an alternative program to BioQuant for bone >> histomorphometry. We need to quantify the number of osteoblasts / >> osteoclasts per bone surface area as well as the percent surface area >> occupied by those cells. We have a computer with BioQuant on it available, >> but we find the software to be incredibly clunky and often nearly >> impossible >> to use. Based on my limited attempts to use it, it very well might rank as >> one of the worst user interfaces I've ever seen, and I was trained in >> computer science and have seen my fair share of horrible software (I'm >> looking at you, Lotus Notes). >> >> Anyhow, any suggestions on a (preferably cheap / free) replacement for >> doing >> simple analysis or how to make BioQuant less painful would be very >> helpful. >> >> Thanks, >> Adam >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> From soofias2 <@t> yahoo.com Mon Feb 8 10:31:41 2010 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Mon Feb 8 10:58:33 2010 Subject: [Histonet] Frozen Sections Slides per Day Question In-Reply-To: <783841.82216.qm@web65710.mail.ac4.yahoo.com> Message-ID: <735076.56429.qm@web112518.mail.gq1.yahoo.com> Hi all, Thank you so much! for all of your suggestions. ? ?Rene, How many slides per?case, if FS standard is 15 min/case? ? Thanks again. ? Soofia --- On Sat, 2/6/10, Rene J Buesa wrote: From: Rene J Buesa Subject: RE: [Histonet] Frozen Sections Slides per Day Question To: "tahseen@brain.net.pk" , "soofia siddiqui" , "Barry RRittman" Cc: "histonet@lists.utsouthwestern.edu" Date: Saturday, February 6, 2010, 9:19 AM FS standard is 15 minutes/case. Ren? J. --- On Sat, 2/6/10, Rittman, Barry R wrote: From: Rittman, Barry R Subject: RE: [Histonet] Frozen Sections Slides per Day Question To: "tahseen@brain.net.pk" , "soofia siddiqui" Cc: "histonet@lists.utsouthwestern.edu" Date: Saturday, February 6, 2010, 8:03 AM While I no longer cut frozen sections, I did so for several years. The number of blocks and sections you cut depends on so many factors including the type of tissue sectioned,? changing knife and roll plate positions, time to seal and store blocks after cutting, your expertise? etc. So that, depending on circumstances, the number can vary considerably from lab to lab. What mostly concerned me about your email was that you would be doing this all day. There are so many injuries that can be caused by repetitive tasks in histology, most commonly cutting of frozen and paraffin wax sections. Apart from this is the potential for errors that occur with continuous repetitive tasks and increases with the length of time period in which these are done.. I would recommend that multitasking with some other tasks alternating with the frozen sections Just my opinion. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tahseen@brain.net.pk [tahseen@brain.net.pk] Sent: Saturday, February 06, 2010 3:38 AM To: soofia siddiqui Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen Sections Slides per Day Question Dear Soofia Sissiqui, 25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units code # 5019 entire day ( 8 hours 480 minte) Muhammad Tahseen Histology Supervisor SKMTH&RC Lahore Pakistan > Hi dear histology experts, > I will greatly appreciate if somebody can let me know the estimated number > of slides (with 3 sections per slide) per day on average a lab technician > can cut (of frozen tissue). > > I am a lab technician ( not a histotech) and work alone in a highly > complex testing specialzed low volume dermatology lab.My job > description includes 30 % of immunohistochemistry related dutites.? I cut > skin frozen sections and do immunohistochemistry? manually for several > years . My routiene panel consists of 12 antibodies for? T-cells? surface > markers. Ocassionally? I add another panel of 8 antibodies for B-Cells. > I am very slow in cutting sections and strugle a lot to get good sections. > If I spend entire day ( 8 hours) just? cutting? 3-4 section on each slide > with slow speed. What? number of slides should be considered as efficent > cutting? > What number of blocks (12 slides for each block with 3-4 sections) should > I finish in one day? > > Help me please if you can. Thanks. Soofia > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Feb 8 12:12:39 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Feb 8 12:12:44 2010 Subject: [Histonet] H&E for urates Message-ID: What modifications are made to the H&E stain to demonstrate urates? Thank you, Jennifer From vavalos <@t> allergydermatology.com Mon Feb 8 12:13:20 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Mon Feb 8 12:13:24 2010 Subject: [Histonet] SLIDE LABELS Message-ID: <000001caa8ea$658f3c60$30adb520$@com> Where does everyone buy slide labels from? Am interested in printing out our own instead of getting them pre printed. Looking for 8 ? x11 sheet of 15/16 x 15/16 labels. White and colored. Thanks in advance for any suggestions. Vanessa Avalos Histology Fax: 602-277-2134 From rjbuesa <@t> yahoo.com Mon Feb 8 11:57:01 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 8 12:20:53 2010 Subject: [Histonet] Frozen Sections Slides per Day Question In-Reply-To: <735076.56429.qm@web112518.mail.gq1.yahoo.com> Message-ID: <486857.89664.qm@web65708.mail.ac4.yahoo.com> The "norm" is?2 (max. 3) slides per case. Your figure of 25 FS in 8 hours = 19.2 min/FS but you do a lot more that just 2-3 slides/case. Ren? J --- On Mon, 2/8/10, soofia siddiqui wrote: From: soofia siddiqui Subject: RE: [Histonet] Frozen Sections Slides per Day Question To: "tahseen@brain.net.pk" , "Barry RRittman" , "Rene J Buesa" Cc: "histonet@lists.utsouthwestern.edu" Date: Monday, February 8, 2010, 11:31 AM Hi all, Thank you so much! for all of your suggestions. ? ?Rene, How many slides per?case, if FS standard is 15 min/case? ? Thanks again. ? Soofia --- On Sat, 2/6/10, Rene J Buesa wrote: From: Rene J Buesa Subject: RE: [Histonet] Frozen Sections Slides per Day Question To: "tahseen@brain.net.pk" , "soofia siddiqui" , "Barry RRittman" Cc: "histonet@lists.utsouthwestern.edu" Date: Saturday, February 6, 2010, 9:19 AM FS standard is 15 minutes/case. Ren? J. --- On Sat, 2/6/10, Rittman, Barry R wrote: From: Rittman, Barry R Subject: RE: [Histonet] Frozen Sections Slides per Day Question To: "tahseen@brain.net.pk" , "soofia siddiqui" Cc: "histonet@lists.utsouthwestern.edu" Date: Saturday, February 6, 2010, 8:03 AM While I no longer cut frozen sections, I did so for several years. The number of blocks and sections you cut depends on so many factors including the type of tissue sectioned,? changing knife and roll plate positions, time to seal and store blocks after cutting, your expertise? etc. So that, depending on circumstances, the number can vary considerably from lab to lab. What mostly concerned me about your email was that you would be doing this all day. There are so many injuries that can be caused by repetitive tasks in histology, most commonly cutting of frozen and paraffin wax sections. Apart from this is the potential for errors that occur with continuous repetitive tasks and increases with the length of time period in which these are done.. I would recommend that multitasking with some other tasks alternating with the frozen sections Just my opinion. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tahseen@brain.net.pk [tahseen@brain.net.pk] Sent: Saturday, February 06, 2010 3:38 AM To: soofia siddiqui Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen Sections Slides per Day Question Dear Soofia Sissiqui, 25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units code # 5019 entire day ( 8 hours 480 minte) Muhammad Tahseen Histology Supervisor SKMTH&RC Lahore Pakistan > Hi dear histology experts, > I will greatly appreciate if somebody can let me know the estimated number > of slides (with 3 sections per slide) per day on average a lab technician > can cut (of frozen tissue). > > I am a lab technician ( not a histotech) and work alone in a highly > complex testing specialzed low volume dermatology lab.My job > description includes 30 % of immunohistochemistry related dutites.? I cut > skin frozen sections and do immunohistochemistry? manually for several > years . My routiene panel consists of 12 antibodies for? T-cells? surface > markers. Ocassionally? I add another panel of 8 antibodies for B-Cells. > I am very slow in cutting sections and strugle a lot to get good sections. > If I spend entire day ( 8 hours) just? cutting? 3-4 section on each slide > with slow speed. What? number of slides should be considered as efficent > cutting? > What number of blocks (12 slides for each block with 3-4 sections) should > I finish in one day? > > Help me please if you can. Thanks. Soofia > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Mon Feb 8 12:20:42 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Mon Feb 8 12:20:56 2010 Subject: [Histonet] SLIDE LABELS In-Reply-To: <000001caa8ea$658f3c60$30adb520$@com> References: <000001caa8ea$658f3c60$30adb520$@com> Message-ID: St. John's Company 1-800-435-4242 Jayne Rutledge The labels are high quality but no where near as expensive as American Mastertech... in fact, they are 30% less!!! They have lots of other supplies and get back within minutes if they are on the other line. Jayne is very sweet too! Good luck... I swear by these labels.... Maria Katleba HT (ASCP) MS Pathology Dept. Mgr Queen of the valley Medical Center Napa CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Monday, February 08, 2010 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SLIDE LABELS Where does everyone buy slide labels from? Am interested in printing out our own instead of getting them pre printed. Looking for 8 ? x11 sheet of 15/16 x 15/16 labels. White and colored. Thanks in advance for any suggestions. Vanessa Avalos Histology Fax: 602-277-2134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Timothy.Morken <@t> ucsfmedctr.org Mon Feb 8 12:42:04 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Mon Feb 8 12:42:19 2010 Subject: [Histonet] Slide baking before IHC In-Reply-To: <7B30AD8C22FB469EB4BF70989584020F@prueggihctechlt> References: <1AAF670737F193429070841C6B2ADD4C0121DF474F@EXMBMCB15.ucsfmedicalcenter.org> <7B30AD8C22FB469EB4BF70989584020F@prueggihctechlt> Message-ID: <1AAF670737F193429070841C6B2ADD4C0121DF4BF9@EXMBMCB15.ucsfmedicalcenter.org> Much of the information on slide drying has to be gleaned from books and is mostly the personal experience of various authors. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Sunday, February 07, 2010 7:55 AM To: Morken, Tim; 'Pat Laurie'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide baking before IHC I concur with Tim, this paper is a good reference for slide drying temps, as with everything else there is no rule that works for every antigen, I play it safe and try very hard to air dry draining until there is no water left on or under the section, and then I bake at temps not above 60dc for most things, but when it comes to bone and cartilage, I drain and air dry them and then lay them flat on heat plate at 45 dc overnight when possible. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, February 05, 2010 10:24 AM To: Pat Laurie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide baking before IHC Here is the reference: Effect of Slide Drying at 80Deg C on Immunohistochemistry A.F. Henwood J Histotechnology, VOl. 28, no. 1, March 2005 If you are an NSH member you can email the NSH office and ask for a pdf reprint. The author compares heating the slides at 80C for seven hours to one hour at 65C. Some antigens were adversely affected, some were not. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Friday, February 05, 2010 12:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide baking before IHC Histonet, I have heard rather anecdotally that if you bake slides at high temps (75 degrees to 80 degrees) before IHC for a long period of time (several hours to days), you may affect antigenicity for some antibodies. Has there been any study done about this? Also, what if it is a high temp (around 75 degrees C) for just 20 minutes? Or a low temperature for a long period of time? Thanks in advance for your input. -- Patrick Laurie HT(ASCP)QIHC plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nathanael <@t> bioquant.com Mon Feb 8 13:01:36 2010 From: nathanael <@t> bioquant.com (Nathanael Reveal) Date: Mon Feb 8 13:02:03 2010 Subject: [Histonet] Re: Alternatives to BioQuant for Bone Histomorphometry Message-ID: Hi Adam, Sorry the BIOQUANT is causing so much frustration for you! It certainly sounds like the protocol that was suggested to you isn't best tool for your work. Could I ask you to send me an image of the tissue you need to analyze? The thresholding tools tend to work best with non-demineralized tissue stained with one of the trichrome stains. You're right the thresholding isn't great with H&E on TRAP. There are some fairly straightforward manual tracing protocols that might do better as in: choose the Bone Surface array and trace it on the image. Choose the Oc.N array and click the osteoclasts in the image. To help document a more relevant protocol, we can put together a custom training video for you kind of like the one here: http://download.bioquant.com/digital-pathology I'd be glad to handle the support details off the list if you like. Best, Nathanael --- Nathanael Reveal, President BIOQUANT Image Analysis Corporation 5611 Ohio Avenue, Nashville, TN 37209 phone: 615-350-7866, toll free: 800-221-0549, fax: 615-350-7282 email: nathanael@bioquant.com, web: www.bioquant.com My life is an indivisible whole, and all my activities run into one another and they have their rise in my insatiable love of humanity. Mohandas K. Gandhi --QUOTE-- Message: 12 Date: Fri, 5 Feb 2010 16:38:05 -0600 From: "Adam ." Subject: [Histonet] Alternatives to BioQuant for Bone Histomorphometry To: histonet@lists.utsouthwestern.edu Message-ID: <858249121002051438h85948f4h896f6676fad94f3d@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi all, I am looking for an alternative program to BioQuant for bone histomorphometry. We need to quantify the number of osteoblasts / osteoclasts per bone surface area as well as the percent surface area occupied by those cells. We have a computer with BioQuant on it available, but we find the software to be incredibly clunky and often nearly impossible to use. Based on my limited attempts to use it, it very well might rank as one of the worst user interfaces I've ever seen, and I was trained in computer science and have seen my fair share of horrible software (I'm looking at you, Lotus Notes). Anyhow, any suggestions on a (preferably cheap / free) replacement for doing simple analysis or how to make BioQuant less painful would be very helpful. Thanks, Adam From rjr6 <@t> psu.edu Mon Feb 8 13:06:49 2010 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Mon Feb 8 13:07:13 2010 Subject: [Histonet] SLIDE LABELS In-Reply-To: <000001caa8ea$658f3c60$30adb520$@com> References: <000001caa8ea$658f3c60$30adb520$@com> Message-ID: I like the ones that Diversified Biotech has. The place I used to get them no longer has slide labels. I called Diversified Biotech and they sent me a sample to see if it will work and I am happy with it. 800-796-9199 the item number is MISL-1000 Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Monday, February 08, 2010 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SLIDE LABELS Where does everyone buy slide labels from? Am interested in printing out our own instead of getting them pre printed. Looking for 8 ? x11 sheet of 15/16 x 15/16 labels. White and colored. Thanks in advance for any suggestions. Vanessa Avalos Histology Fax: 602-277-2134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mturner <@t> carisdx.com Mon Feb 8 13:07:23 2010 From: mturner <@t> carisdx.com (Turner, Mark) Date: Mon Feb 8 13:07:28 2010 Subject: [Histonet] Slide Drying Before IHC Message-ID: A little twist on this topic I would like a few opinions on. We just received 2 new drying ovens that are gravity convection versus the older two which use a fan to circulate air. In the past I have used both and noticed no appreciable difference, even though this constitutes a process inconsistency. Has anyone noticed a difference in final results due to the different methods of drying? Mark Turner, HT(ASCP) QIHC Supervisor IHC Target Now MPI Caris Life Sciences 445 N. 5th Street Phoenix, AZ 85004 Cell: 602-309-5084 Direct 602-358-8913 Fax: 602-358-8919 mturner@carisdx.com From JMacDonald <@t> mtsac.edu Mon Feb 8 13:42:19 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Feb 8 13:42:22 2010 Subject: [Histonet] gurney cover Message-ID: Does anyone know of a transparent plastic cover that goes over a gurney?. We use cadavers in the anatomy lab and would like to display the cadaver but prevent students from touching and evaporation during practical exams. Thank you, Jennifer From liz <@t> premierlab.com Mon Feb 8 13:53:31 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Feb 8 13:53:38 2010 Subject: [Histonet] gurney cover In-Reply-To: Message-ID: I'm not sure of a plastic transparent one, but MOPEC sells the covers. This goes way back in the early 90's but I think they have a person that makes them for them, so they may possibly be able to make you one that is transparent. Its worth a try and a phone call. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, February 08, 2010 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] gurney cover Does anyone know of a transparent plastic cover that goes over a gurney?. We use cadavers in the anatomy lab and would like to display the cadaver but prevent students from touching and evaporation during practical exams. Thank you, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Mon Feb 8 13:57:14 2010 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Feb 8 13:57:24 2010 Subject: [Histonet] gurney cover In-Reply-To: References: Message-ID: <4B706C9A.2030906@umdnj.edu> clear plastic sheeting (available at hardware stores for painting jobs, tarps, etc) might be an easier-to-find and more cost-effective alternative? Liz Chlipala wrote: > I'm not sure of a plastic transparent one, but MOPEC sells the covers. > This goes way back in the early 90's but I think they have a person that > makes them for them, so they may possibly be able to make you one that > is transparent. Its worth a try and a phone call. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, Colorado 80308 > office (303) 682-3949 > fax (303) 682-9060 > www.premierlab.com > > > Ship to Address: > 1567 Skyway Drive, Unit E > Longmont, Colorado 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer > MacDonald > Sent: Monday, February 08, 2010 12:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] gurney cover > > Does anyone know of a transparent plastic cover that goes over a > gurney?. > We use cadavers in the anatomy lab and would like to display the cadaver > > but prevent students from touching and evaporation during practical > exams. > Thank you, > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From raj <@t> bluemarble.net Mon Feb 8 14:44:43 2010 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Mon Feb 8 14:45:09 2010 Subject: [Histonet] pc labs Message-ID: <73D43CD36A454BBCA9B10239536259BB@CHURCH> Sorry if I have caused all of this confusion. First I am very disappointed in some of my fellow HT or HTL. I have worked in histology long enough to realize their are money hungry doctors in hospital settings and private labs. Money is their first concern. Also I have experienced it doesn't matter what the histology dept. education is HT, HTL, BS or PHD. Just because those initials are behind your name does not give one the knowledge or experience to be qualified to be in a histology lab.I am one that is in favor to see the need for education, but that does not always mean the tech has the knowledge. I have also seen some techs that have a hard time passing the written section of the ASCP exam. Sometimes they just have a hard time with testing. As for me I don't want to work for any Doctor in a hospital or private lab that their concern is only on the money. I will only associate my name with the best (this being quality patient care). I think we have all seen medicine is not what it use to be. Insurance companies and the government have made changes. Do we agree with it all NO. As for private labs you are not well educated on them, the private lab has regulations that they must meet and are CLIA inspected some are CAP and JACO. I do realized there are labs out there that are meat markets. I sent this request because I thought this was the intent of the Histnet, sharing valuable information and experiences. Sorry if this offend some of you. Thanks raj From estellamireles <@t> gmail.com Mon Feb 8 15:41:43 2010 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Mon Feb 8 15:41:49 2010 Subject: [Histonet] Histology Job - Houston (East Suburb) Message-ID: FT Histo Job. Routine Work. Heavy Bx's Frozens, Special Stains Day Shift. Weekends off. Contact Herman Hospital Website - Northeast Location Further Info 281 540 6275 From louise.renton <@t> gmail.com Tue Feb 9 00:40:41 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Feb 9 00:40:47 2010 Subject: [Histonet] Alternatives to BioQuant for Bone Histomorphometry In-Reply-To: <4B6FC99A0200008B0001E1C7@grpwise.novell.upei.ca> References: <858249121002051438h85948f4h896f6676fad94f3d@mail.gmail.com> <4B6FC99A0200008B0001E1C7@grpwise.novell.upei.ca> Message-ID: I use a system called analySIS (for Windows)- marketed here by Olympus. We use this for percentage bone calculations, linear measurements and touch counting. The data is immediately put into a spread sheet and you have an automatic stats facility On Mon, Feb 8, 2010 at 2:21 PM, Kathleen Jones wrote: > Hi Adam > > Have you checked out ImageJ? It's a free downloadable program that I > have used for alveolar morphometry. Fairly user friendly, more so than > BioQuant, although not quite as thorough. > > Good Luck > Kathy > > > Kathleen Jones > Research Technician > Pathology/Microbiology > AVC - UPEI > (902)566-0595 > > > >>> "Adam ." 2/5/2010 6:38 PM >>> > Hi all, > > I am looking for an alternative program to BioQuant for bone > histomorphometry. We need to quantify the number of osteoblasts / > osteoclasts per bone surface area as well as the percent surface area > occupied by those cells. We have a computer with BioQuant on it > available, > but we find the software to be incredibly clunky and often nearly > impossible > to use. Based on my limited attempts to use it, it very well might rank > as > one of the worst user interfaces I've ever seen, and I was trained in > computer science and have seen my fair share of horrible software (I'm > looking at you, Lotus Notes). > > Anyhow, any suggestions on a (preferably cheap / free) replacement for > doing > simple analysis or how to make BioQuant less painful would be very > helpful. > > Thanks, > Adam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Dorothy.L.Webb <@t> HealthPartners.Com Tue Feb 9 12:12:02 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Feb 9 12:12:16 2010 Subject: [Histonet] Slide labels Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C57696D6B7@HPEMX3.HealthPartners.int> Shamrock and Coridian are 2 very good companies to buy labels from and they will work with any size you need!! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From 41dmb41 <@t> gmail.com Tue Feb 9 12:22:16 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Tue Feb 9 12:22:32 2010 Subject: [Histonet] Dako Rep Message-ID: Does anyone have the name and contact info for the Dako rep in the Atlanta area? Thanks, Drew Sent from my iPhone From alyssa <@t> alliedsearchpartners.com Tue Feb 9 12:31:25 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Feb 9 12:31:29 2010 Subject: [Histonet] Question-HPD March 10th Message-ID: Hello, I was wondering if there is anyone out there who knows of any events or celebrations for Histotechnology Professonals Day on March 10th in or around the Orlando, FL area?? --Alyssa Peterson From JWeems <@t> sjha.org Tue Feb 9 12:31:25 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Feb 9 12:33:03 2010 Subject: [Histonet] Dako Rep In-Reply-To: References: Message-ID: Here ya go Caren.Miears@dako.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, February 09, 2010 13:22 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Rep Does anyone have the name and contact info for the Dako rep in the Atlanta area? Thanks, Drew Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From nancy_schmitt <@t> pa-ucl.com Tue Feb 9 12:38:14 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Feb 9 12:42:20 2010 Subject: [Histonet] light staining Message-ID: <737BD0BF52F0744B96B74B61756AC064416583C542@hestia.ad.pa-ucl.com> Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? Thanks for your help Nancy Schmitt United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From DKBoyd <@t> chs.net Tue Feb 9 12:50:16 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Feb 9 12:49:08 2010 Subject: [Histonet] light staining In-Reply-To: <737BD0BF52F0744B96B74B61756AC064416583C542@hestia.ad.pa-ucl.com> Message-ID: Do you use city water in your wash stations? Have you had more snow/ice than usual? If so the Water Tx Plant is putting more chlorine in the water than usual. This will affect you H&E. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From histonet.nospam <@t> vneubert.com Tue Feb 9 12:50:24 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Tue Feb 9 12:54:36 2010 Subject: [Histonet] light staining In-Reply-To: <737BD0BF52F0744B96B74B61756AC064416583C542@hestia.ad.pa-ucl.com> References: <737BD0BF52F0744B96B74B61756AC064416583C542@hestia.ad.pa-ucl.com> Message-ID: <4B71AE70.6070602@vneubert.com> Check pH of your washing water. pH too low will result in not staining at all. I'd clean the stainer as well. > Hello Histonetters > > We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? > > Thanks for your help > Nancy Schmitt > United Clinical Laboratories > Dubuque, IA > > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mtighe <@t> trudeauinstitute.org Tue Feb 9 13:57:15 2010 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Tue Feb 9 13:57:29 2010 Subject: [Histonet] Liver tissue Message-ID: <4B71779D.26E4.00EE.0@trudeauinstitute.org> I am having trouble cutting FFPE liver sections. I have embedded the tissue manually going from fomalin to water to increasing ethanol to xylene and to paraffin. I can get good sections by soaking the block with ice cold water before taking a few sections but then have to re-soak in order to take more sections. If I do not soak the tissue on the chuck the liver crumbles and falls out of the paraffin. The soaking and sectioning is very time consuming. Does anyone have an idea what might be the problem? Thanks for any help!! Mike From Allison_Scott <@t> hchd.tmc.edu Tue Feb 9 14:36:00 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Tue Feb 9 14:36:04 2010 Subject: [Histonet] Progressive counseling Message-ID: <1872B4A455B7974391609AD8034C79FC8BD6BC@LBEXCH01.hchd.local> Hello to all in histoland. I had to come out of the box for this question. What types of criteria do you have for verbal and written counseling, as pertaining to histology functions. Any help in this will be helpful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From histonet.nospam <@t> vneubert.com Tue Feb 9 14:38:37 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Tue Feb 9 14:39:08 2010 Subject: [Histonet] Liver tissue In-Reply-To: <4B71779D.26E4.00EE.0@trudeauinstitute.org> References: <4B71779D.26E4.00EE.0@trudeauinstitute.org> Message-ID: <4B71C7CD.5040506@vneubert.com> Longer dehydration, longer paraffination is what I would suggest. Splintering occured when I used freezing spray and the tissue got too cold. Try to increase humidity while sectioning (gently breathe on the block while sectioning). Should help against crumbling. Good luck, post your dehydration schedule please! > I am having trouble cutting FFPE liver sections. I have embedded the tissue manually going from fomalin to water to increasing ethanol to xylene and to paraffin. I can get good sections by soaking the block with ice cold water before taking a few sections but then have to re-soak in order to take more sections. If I do not soak the tissue on the chuck the liver crumbles and falls out of the paraffin. The soaking and sectioning is very time consuming. Does anyone have an idea what might be the problem? > > Thanks for any help!! > Mike > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Tue Feb 9 14:45:46 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 9 14:51:07 2010 Subject: [Histonet] Progressive counseling In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD6BC@LBEXCH01.hchd.local> Message-ID: <274980.78549.qm@web65706.mail.ac4.yahoo.com> The criteria is simple = whenever what is described in the Competencies or Standards of Performance is not met by the histotech. 1 verbal ? 2nd verbal ? written counseling. Ren? J. --- On Tue, 2/9/10, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Progressive counseling To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 9, 2010, 3:36 PM Hello to all in histoland.? I had? to come out of the box for this question.? What types of criteria do you have for verbal and? written counseling, as pertaining to histology functions.? Any help in this will be helpful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cehickm2 <@t> olemiss.edu Tue Feb 9 15:04:11 2010 From: cehickm2 <@t> olemiss.edu (Cammi Thornton) Date: Tue Feb 9 15:04:24 2010 Subject: [Histonet] Gill sectioning tips? Message-ID: <20100209210309.C26CF56285@umavas4.olemiss.edu> Hi everyone, We have been trying to section gills lately and have come across quite a few problems. Does anybody have any helpful hints for fixing/sectioning gills? We are going to try to separate each individual arch but the fish we use are very small so we don't know how that will go. Thanks, Cammi Cammi Hickman Thornton Research & Development Chemist University of Mississippi School of Pharmacy Department of Pharmacology 200 Old Power Plant University, Mississippi 38677 Phone - 662-915-7612, Fax - 662-915-5148 From rjbuesa <@t> yahoo.com Tue Feb 9 15:27:52 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 9 15:27:56 2010 Subject: [Histonet] Gill sectioning tips? In-Reply-To: <20100209210309.C26CF56285@umavas4.olemiss.edu> Message-ID: <668604.62942.qm@web65707.mail.ac4.yahoo.com> If with "small" you refer to "juvenile" is different to an "small adult". Both will have supporting material for the gill arches but in "juveniles" they should be easy to section after a good infiltration and using an adequate paraffin wax to meet the tissue resistance. If you are talking about a small adult, the situation is different because an adult will have more compact support material for the arches. One solution would be metacrylate infiltration, and another softening of the support tissue before processing. Ren? J. --- On Tue, 2/9/10, Cammi Thornton wrote: From: Cammi Thornton Subject: [Histonet] Gill sectioning tips? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 9, 2010, 4:04 PM Hi everyone, We have been trying to section gills lately and have come across quite a few problems.? Does anybody have any helpful hints for fixing/sectioning gills?? We are going to try to separate each individual arch but the fish we use are very small so we don't know how that will go. Thanks, Cammi Cammi Hickman Thornton Research & Development Chemist University of Mississippi School of Pharmacy Department of Pharmacology 200 Old Power Plant University, Mississippi 38677 Phone - 662-915-7612, Fax - 662-915-5148 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raj <@t> bluemarble.net Tue Feb 9 17:28:08 2010 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Tue Feb 9 17:28:33 2010 Subject: [Histonet] Crystal indentification Message-ID: <61A1DF3777FF4186A194D645BC153B5C@CHURCH> Question, when doing a smear for crystal indentification (Gout) are you retaining the slide and for how long. How are you preserving it? Thank raj From AnthonyH <@t> chw.edu.au Tue Feb 9 18:23:40 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Feb 9 18:23:47 2010 Subject: [Histonet] light staining In-Reply-To: <737BD0BF52F0744B96B74B61756AC064416583C542@hestia.ad.pa-ucl.com> Message-ID: I would have a look at the haematoxylin stained section before you differentiate. Does it look OK? If it does then do not differentiate, blue only. (also someone has not accidently put acid in the blueing solution? Check with apH papers). You might try extending the Hx time. If it still looks pale after the eosin, then the acid in the eosin is continuing the differentiation process. Extend your Hx staining time & decrease the eosin time. If it is a young Hx then you might find that the staining improves with time (ie it "ripens"). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, 10 February 2010 5:38 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] light staining Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? Thanks for your help Nancy Schmitt United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From CIngles <@t> uwhealth.org Tue Feb 9 19:16:04 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Feb 9 19:21:09 2010 Subject: [Histonet] Mohs FYI Message-ID: Hey gang: Here with some good news for once. I found out today that we WON our appeal to JCAHO over our tissue retaining time. They are giving Mohs labs an OK not to have to retain our tissue!! I guess it is 'processed' tissue instead of 'gross' tissue. Retaining it doesn't does not help patient care as it is not fixed and deteriorates (and there are already slides of it anyway.) So all you fellow Mohs techs can start breathing (and getting rid of your tissue) again. Claire From macveigh <@t> usc.edu Tue Feb 9 19:47:08 2010 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Tue Feb 9 19:47:11 2010 Subject: [Histonet] Re: Liver Tissue Message-ID: <002401caa9f2$f49e5f40$5c237d80@DFS66DD1> Soak the faced block in room temp. water for few minutes. This will speed the intake of water. I can usually cut it at room temp if I am careful. If your paraffin is soft then you will need to soak it in room temp water first and then put ice on it and cut it. Pick up the ribbon and place on room temp dist water. While you are picking up and stretching the sections, put piece of cotton soaked in water on the face of the block as it is attached to the microtome. This way it will be soaking in place and you won't be loosing nice sections while adjusting the angle for the next ribbon. By the time you pick up all good sections, the block will be ready to be cut again. If you have good size liver, you can soak it for much longer - 5-10 min, before you start sectioning. This way the water will penetrate dipper and you will be able to easily get a very long ribbon. Good luck Michelle Aloni BS HTL Research specialist USC Keck School of Medicine, LA CA From cpyse <@t> x-celllab.com Wed Feb 10 09:41:50 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Feb 10 09:44:29 2010 Subject: [Histonet] light staining In-Reply-To: <737BD0BF52F0744B96B74B61756AC064416583C542@hestia.ad.pa-ucl.com> References: <737BD0BF52F0744B96B74B61756AC064416583C542@hestia.ad.pa-ucl.com> Message-ID: <000001caaa67$8ff916a0$afeb43e0$@com> Check your decolorizer if you are using one. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, February 09, 2010 1:38 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] light staining Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? Thanks for your help Nancy Schmitt United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Feb 10 10:40:20 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Feb 10 10:40:27 2010 Subject: [Histonet] Gill sectioning tips? Message-ID: Are your specimens from cartilaginous or bony fishes? Ages ago I worked with head and "neck" specimens of quite big (10 cm) goldfishes (bony) in a study of optic nerve regeneration. Decalcification after adequate formaldehyde fixation permitted easy cutting of near-serial paraffin sections containing bone, brain and incidentally gills, skin (which has bony scales) and other entertaining things not seen in sections of small people or other little mammals. I don't know how to soften really tough cartilage. Does anyone? John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Cammi Thornton Date: Tuesday, February 9, 2010 16:05 Subject: [Histonet] Gill sectioning tips? To: histonet@lists.utsouthwestern.edu > Hi everyone, > We have been trying to section gills lately and have come across > quite a few problems. Does anybody have any helpful hints > for > fixing/sectioning gills? We are going to try to separate > each > individual arch but the fish we use are very small so we don't > know > how that will go. > Thanks, > Cammi > > Cammi Hickman Thornton > Research & Development Chemist > University of Mississippi > School of Pharmacy > Department of Pharmacology > 200 Old Power Plant > University, Mississippi 38677 > Phone - 662-915-7612, Fax - 662-915-5148 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From STapper <@t> smdc.org Wed Feb 10 10:54:04 2010 From: STapper <@t> smdc.org (Tapper, Sheila J.) Date: Wed Feb 10 10:54:10 2010 Subject: [Histonet] Peloris Processor Users Message-ID: Could you please share your experiences with me? We have had this processor in our facility since 2007, but I am curious about other facilities experiences. I thank you in advance, Sheila Tapper HT(ASCP) Anatomic Pathology Supervisor SMDC Clinical Laboratory 407 East First Street Duluth, MN 55804 This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From NSEARCY <@t> swmail.sw.org Wed Feb 10 11:58:21 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Wed Feb 10 11:58:34 2010 Subject: [Histonet] Xylene Recycler Message-ID: <4B729F5C.5D38.00EF.0@swmail.sw.org> Anyone using the new CBG instrument? Am having some issues with "clogged lines" - which we never had with the older model. Really don't want to change processes - which is what they are asking us to do. Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From hisham.sys <@t> gmail.com Wed Feb 10 12:15:35 2010 From: hisham.sys <@t> gmail.com (Hisham Mohammed) Date: Wed Feb 10 12:15:43 2010 Subject: [Histonet] problem with subbed slides Message-ID: <3b7d8d521002101015t2d08a94cn6e1267670909d7e@mail.gmail.com> dear histonetters following gelatin subbing of big glass slides we observed numerous white patches in almost the entire batch of slides subbed. what could be the remedy to remove these white patches. waiting for a practical solution. with regards hisham mohammed senior research centre national brain research centre From DKnutson <@t> primecare.org Wed Feb 10 12:16:50 2010 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Wed Feb 10 12:17:45 2010 Subject: [Histonet] Peloris processor Message-ID: <4F0B7161A6CD524FAD8017D52E1553400D2B6A58@exchangent> We are also interested in other's experiences with the Peloris processor. We are considering a rapid tissue processor and would like to hear from others who have rapid tissue processors. What type do you have? And please share your pros and cons. Thank you very much for your help. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org From j_amiri <@t> modares.ac.ir Wed Feb 10 13:28:29 2010 From: j_amiri <@t> modares.ac.ir (Jamshid Amiri Moghaddam) Date: Wed Feb 10 13:27:00 2010 Subject: [Histonet] RE: Liver Tissue Message-ID: <000001caaa87$39b1f030$ad15d090$@ac.ir> I have problem like Mike, my tissue samples were fixed one year ago in Boine and reserved in 70% ethanol. tissues are too hard. I decreased dehydration time and could take few good section, but after coloration, I couldn't take good photo from those. The sections (diameter: 4 micron) are absorbed too color and the photos was became sombrous. these tissue are corrupt? Does anyone have an idea, how can I solve the problem? Thanks for any help!! Jamshid Today's Topics: 1. Slide labels (Webb, Dorothy L) 2. Dako Rep (Drew Meyer) 3. Question-HPD March 10th (Alyssa Peterson) 4. RE: Dako Rep (Weems, Joyce) 5. light staining (Nancy Schmitt) 6. Re: light staining (DKBoyd@chs.net) 7. Re: light staining (V. Neubert) 8. Liver tissue (Mike Tighe) 9. Progressive counseling (Scott, Allison D) 10. Re: Liver tissue (V. Neubert) 11. Re: Progressive counseling (Rene J Buesa) 12. Gill sectioning tips? (Cammi Thornton) 13. Re: Gill sectioning tips? (Rene J Buesa) 14. Crystal indentification (Rebecca Johnson) 15. RE: light staining (Tony Henwood) 16. Mohs FYI (Ingles Claire ) 17. Re: Liver Tissue (Michelle MacVeigh-Aloni) 18. RE: light staining (Cynthia Pyse) 19. Re: Gill sectioning tips? (John Kiernan) 20. Peloris Processor Users (Tapper, Sheila J.) 21. Xylene Recycler (Nita Searcy) ---------------------------------------------------------------------- Message: 1 Date: Tue, 9 Feb 2010 12:12:02 -0600 From: "Webb, Dorothy L" Subject: [Histonet] Slide labels To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C57696D6B7@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Shamrock and Coridian are 2 very good companies to buy labels from and they will work with any size you need!! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 2 Date: Tue, 9 Feb 2010 13:22:16 -0500 From: Drew Meyer <41dmb41@gmail.com> Subject: [Histonet] Dako Rep To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes Does anyone have the name and contact info for the Dako rep in the Atlanta area? Thanks, Drew Sent from my iPhone ------------------------------ Message: 3 Date: Tue, 9 Feb 2010 13:31:25 -0500 From: Alyssa Peterson Subject: [Histonet] Question-HPD March 10th To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, I was wondering if there is anyone out there who knows of any events or celebrations for Histotechnology Professonals Day on March 10th in or around the Orlando, FL area?? --Alyssa Peterson ------------------------------ Message: 4 Date: Tue, 9 Feb 2010 13:31:25 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] Dako Rep To: "Drew Meyer" <41dmb41@gmail.com>, Message-ID: Content-Type: text/plain; charset="us-ascii" Here ya go Caren.Miears@dako.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, February 09, 2010 13:22 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Rep Does anyone have the name and contact info for the Dako rep in the Atlanta area? Thanks, Drew Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 5 Date: Tue, 9 Feb 2010 12:38:14 -0600 From: Nancy Schmitt Subject: [Histonet] light staining To: "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: <737BD0BF52F0744B96B74B61756AC064416583C542@hestia.ad.pa-ucl.com> Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? Thanks for your help Nancy Schmitt United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 6 Date: Tue, 9 Feb 2010 13:50:16 -0500 From: DKBoyd@chs.net Subject: Re: [Histonet] light staining To: Nancy Schmitt Cc: "Histonet \(histonet@lists.utsouthwestern.edu\)" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Do you use city water in your wash stations? Have you had more snow/ice than usual? If so the Water Tx Plant is putting more chlorine in the water than usual. This will affect you H&E. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 7 Date: Tue, 09 Feb 2010 19:50:24 +0100 From: "V. Neubert" Subject: Re: [Histonet] light staining To: histonet@lists.utsouthwestern.edu Message-ID: <4B71AE70.6070602@vneubert.com> Content-Type: text/plain; charset=ISO-8859-1 Check pH of your washing water. pH too low will result in not staining at all. I'd clean the stainer as well. > Hello Histonetters > > We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? > > Thanks for your help > Nancy Schmitt > United Clinical Laboratories > Dubuque, IA > > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Tue, 09 Feb 2010 14:57:15 -0500 From: "Mike Tighe" Subject: [Histonet] Liver tissue To: Message-ID: <4B71779D.26E4.00EE.0@trudeauinstitute.org> Content-Type: text/plain; charset=US-ASCII I am having trouble cutting FFPE liver sections. I have embedded the tissue manually going from fomalin to water to increasing ethanol to xylene and to paraffin. I can get good sections by soaking the block with ice cold water before taking a few sections but then have to re-soak in order to take more sections. If I do not soak the tissue on the chuck the liver crumbles and falls out of the paraffin. The soaking and sectioning is very time consuming. Does anyone have an idea what might be the problem? Thanks for any help!! Mike ------------------------------ Message: 9 Date: Tue, 9 Feb 2010 14:36:00 -0600 From: "Scott, Allison D" Subject: [Histonet] Progressive counseling To: Message-ID: <1872B4A455B7974391609AD8034C79FC8BD6BC@LBEXCH01.hchd.local> Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. I had to come out of the box for this question. What types of criteria do you have for verbal and written counseling, as pertaining to histology functions. Any help in this will be helpful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 10 Date: Tue, 09 Feb 2010 21:38:37 +0100 From: "V. Neubert" Subject: Re: [Histonet] Liver tissue To: histonet@lists.utsouthwestern.edu Message-ID: <4B71C7CD.5040506@vneubert.com> Content-Type: text/plain; charset=ISO-8859-1 Longer dehydration, longer paraffination is what I would suggest. Splintering occured when I used freezing spray and the tissue got too cold. Try to increase humidity while sectioning (gently breathe on the block while sectioning). Should help against crumbling. Good luck, post your dehydration schedule please! > I am having trouble cutting FFPE liver sections. I have embedded the tissue manually going from fomalin to water to increasing ethanol to xylene and to paraffin. I can get good sections by soaking the block with ice cold water before taking a few sections but then have to re-soak in order to take more sections. If I do not soak the tissue on the chuck the liver crumbles and falls out of the paraffin. The soaking and sectioning is very time consuming. Does anyone have an idea what might be the problem? > > Thanks for any help!! > Mike > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 11 Date: Tue, 9 Feb 2010 12:45:46 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Progressive counseling To: histonet@lists.utsouthwestern.edu, Allison DScott Message-ID: <274980.78549.qm@web65706.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 The criteria is simple = whenever what is described in the Competencies or Standards of Performance is not met by the histotech. 1 verbal b 2nd verbal b written counseling. RenC) J. --- On Tue, 2/9/10, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Progressive counseling To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 9, 2010, 3:36 PM Hello to all in histoland.B I hadB to come out of the box for this question.B What types of criteria do you have for verbal andB written counseling, as pertaining to histology functions.B Any help in this will be helpful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.B This e-mail may also be confidential and/or privileged under Texas law.B The e-mail is for the use of only the individual or entity named above.B If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 09 Feb 2010 15:04:11 -0600 From: Cammi Thornton Subject: [Histonet] Gill sectioning tips? To: histonet@lists.utsouthwestern.edu Message-ID: <20100209210309.C26CF56285@umavas4.olemiss.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Hi everyone, We have been trying to section gills lately and have come across quite a few problems. Does anybody have any helpful hints for fixing/sectioning gills? We are going to try to separate each individual arch but the fish we use are very small so we don't know how that will go. Thanks, Cammi Cammi Hickman Thornton Research & Development Chemist University of Mississippi School of Pharmacy Department of Pharmacology 200 Old Power Plant University, Mississippi 38677 Phone - 662-915-7612, Fax - 662-915-5148 ------------------------------ Message: 13 Date: Tue, 9 Feb 2010 13:27:52 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Gill sectioning tips? To: histonet@lists.utsouthwestern.edu, Cammi Thornton Message-ID: <668604.62942.qm@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If with "small" you refer to "juvenile" is different to an "small adult". Both will have supporting material for the gill arches but in "juveniles" they should be easy to section after a good infiltration and using an adequate paraffin wax to meet the tissue resistance. If you are talking about a small adult, the situation is different because an adult will have more compact support material for the arches. One solution would be metacrylate infiltration, and another softening of the support tissue before processing. Reni J. --- On Tue, 2/9/10, Cammi Thornton wrote: From: Cammi Thornton Subject: [Histonet] Gill sectioning tips? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 9, 2010, 4:04 PM Hi everyone, We have been trying to section gills lately and have come across quite a few problems. Does anybody have any helpful hints for fixing/sectioning gills? We are going to try to separate each individual arch but the fish we use are very small so we don't know how that will go. Thanks, Cammi Cammi Hickman Thornton Research & Development Chemist University of Mississippi School of Pharmacy Department of Pharmacology 200 Old Power Plant University, Mississippi 38677 Phone - 662-915-7612, Fax - 662-915-5148 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 9 Feb 2010 18:28:08 -0500 From: "Rebecca Johnson" Subject: [Histonet] Crystal indentification To: "histonet" Message-ID: <61A1DF3777FF4186A194D645BC153B5C@CHURCH> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Question, when doing a smear for crystal indentification (Gout) are you retaining the slide and for how long. How are you preserving it? Thank raj ------------------------------ Message: 15 Date: Wed, 10 Feb 2010 11:23:40 +1100 From: "Tony Henwood" Subject: RE: [Histonet] light staining To: "Nancy Schmitt" , Message-ID: Content-Type: text/plain; charset="us-ascii" I would have a look at the haematoxylin stained section before you differentiate. Does it look OK? If it does then do not differentiate, blue only. (also someone has not accidently put acid in the blueing solution? Check with apH papers). You might try extending the Hx time. If it still looks pale after the eosin, then the acid in the eosin is continuing the differentiation process. Extend your Hx staining time & decrease the eosin time. If it is a young Hx then you might find that the staining improves with time (ie it "ripens"). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, 10 February 2010 5:38 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] light staining Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? Thanks for your help Nancy Schmitt United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 16 Date: Tue, 9 Feb 2010 19:16:04 -0600 From: "Ingles Claire " Subject: [Histonet] Mohs FYI To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hey gang: Here with some good news for once. I found out today that we WON our appeal to JCAHO over our tissue retaining time. They are giving Mohs labs an OK not to have to retain our tissue!! I guess it is 'processed' tissue instead of 'gross' tissue. Retaining it doesn't does not help patient care as it is not fixed and deteriorates (and there are already slides of it anyway.) So all you fellow Mohs techs can start breathing (and getting rid of your tissue) again. Claire ------------------------------ Message: 17 Date: Tue, 9 Feb 2010 17:47:08 -0800 From: "Michelle MacVeigh-Aloni" Subject: [Histonet] Re: Liver Tissue To: Message-ID: <002401caa9f2$f49e5f40$5c237d80@DFS66DD1> Content-Type: text/plain; charset="iso-8859-1" Soak the faced block in room temp. water for few minutes. This will speed the intake of water. I can usually cut it at room temp if I am careful. If your paraffin is soft then you will need to soak it in room temp water first and then put ice on it and cut it. Pick up the ribbon and place on room temp dist water. While you are picking up and stretching the sections, put piece of cotton soaked in water on the face of the block as it is attached to the microtome. This way it will be soaking in place and you won't be loosing nice sections while adjusting the angle for the next ribbon. By the time you pick up all good sections, the block will be ready to be cut again. If you have good size liver, you can soak it for much longer - 5-10 min, before you start sectioning. This way the water will penetrate dipper and you will be able to easily get a very long ribbon. Good luck Michelle Aloni BS HTL Research specialist USC Keck School of Medicine, LA CA ------------------------------ Message: 18 Date: Wed, 10 Feb 2010 10:41:50 -0500 From: "Cynthia Pyse" Subject: RE: [Histonet] light staining To: "'Nancy Schmitt'" , "'Histonet'" Message-ID: <000001caaa67$8ff916a0$afeb43e0$@com> Content-Type: text/plain; charset="us-ascii" Check your decolorizer if you are using one. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, February 09, 2010 1:38 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] light staining Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? Thanks for your help Nancy Schmitt United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 10 Feb 2010 11:40:20 -0500 From: John Kiernan Subject: Re: [Histonet] Gill sectioning tips? To: Cammi Thornton Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; CHARSET=US-ASCII Are your specimens from cartilaginous or bony fishes? Ages ago I worked with head and "neck" specimens of quite big (10 cm) goldfishes (bony) in a study of optic nerve regeneration. Decalcification after adequate formaldehyde fixation permitted easy cutting of near-serial paraffin sections containing bone, brain and incidentally gills, skin (which has bony scales) and other entertaining things not seen in sections of small people or other little mammals. I don't know how to soften really tough cartilage. Does anyone? John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Cammi Thornton Date: Tuesday, February 9, 2010 16:05 Subject: [Histonet] Gill sectioning tips? To: histonet@lists.utsouthwestern.edu > Hi everyone, > We have been trying to section gills lately and have come across > quite a few problems. Does anybody have any helpful hints > for > fixing/sectioning gills? We are going to try to separate > each > individual arch but the fish we use are very small so we don't > know > how that will go. > Thanks, > Cammi > > Cammi Hickman Thornton > Research & Development Chemist > University of Mississippi > School of Pharmacy > Department of Pharmacology > 200 Old Power Plant > University, Mississippi 38677 > Phone - 662-915-7612, Fax - 662-915-5148 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 10 Feb 2010 10:54:04 -0600 From: "Tapper, Sheila J." Subject: [Histonet] Peloris Processor Users To: Message-ID: Content-Type: text/plain; charset="us-ascii" Could you please share your experiences with me? We have had this processor in our facility since 2007, but I am curious about other facilities experiences. I thank you in advance, Sheila Tapper HT(ASCP) Anatomic Pathology Supervisor SMDC Clinical Laboratory 407 East First Street Duluth, MN 55804 This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. ------------------------------ Message: 21 Date: Wed, 10 Feb 2010 11:58:21 -0600 From: "Nita Searcy" Subject: [Histonet] Xylene Recycler To: Cc: Patricia Webster Message-ID: <4B729F5C.5D38.00EF.0@swmail.sw.org> Content-Type: text/plain; charset="us-ascii" Anyone using the new CBG instrument? Am having some issues with "clogged lines" - which we never had with the older model. Really don't want to change processes - which is what they are asking us to do. Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 14 **************************************** __________ Information from ESET NOD32 Antivirus, version of virus signature database 4854 (20100210) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __________ Information from ESET NOD32 Antivirus, version of virus signature database 4855 (20100210) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com From gayle.callis <@t> bresnan.net Wed Feb 10 15:45:49 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Feb 10 15:46:06 2010 Subject: [Histonet] RE: Fish gills Message-ID: <000701caaa9a$6a425ce0$3ec716a0$@callis@bresnan.net> We had superb sections of fish gills after GMA embedding - 2 to 3 um thick sections cut with glass knives, and an H&E stain. The gills were separated but if your samples are very small, no more than 1 mm or so thick, GMA should work. Our departmental photographer won a major scientific photographic contest with her photomicrographs of these gills showing parasites attached to the gills. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 From ccrowder <@t> vetmed.lsu.edu Wed Feb 10 15:44:20 2010 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Wed Feb 10 15:47:50 2010 Subject: [Histonet] Fish Gills Message-ID: Cammi - Our lab processes thousands of fish specimens yearly. Fish of all ages. If the fish is initially fixed in Davidson's there should be no need for decalcification. Older, larger fish, just like mammals, may take further softening. If you have specimens already processed that don't cut well, after facing off soak them in Downy for an hour or so. The first sections should be really good. If you have any questions, contact me directly. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From raj <@t> bluemarble.net Wed Feb 10 17:10:42 2010 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Wed Feb 10 17:11:14 2010 Subject: [Histonet] Crystal identification Message-ID: <5F0F4428AEEA404BBE37CFBAD9F63DAD@CHURCH> Are any of you out there in histoland doing smears for crystal identification (Gout). If so how long are you retaining the slides and how do you preserve the slides? Thanks raj From adesupo2002 <@t> hotmail.com Wed Feb 10 19:15:56 2010 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Wed Feb 10 19:16:01 2010 Subject: [Histonet] LAMBDA AND KAPPA STAINING PROBLEMS Message-ID: Hi, We are having overstaining problems with both our Kappa and Lambda stains using our recently purchased Benchmark XT. Pls, I will appreciate it, if you guys at histoland could offer some suggestions as to taking care of this problem. Thanking you all for your usual cooperation. Adesuyi A, BS,HT(ASCP), HTL(ASCP) _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/ From louise.renton <@t> gmail.com Thu Feb 11 00:49:38 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Feb 11 00:49:44 2010 Subject: [Histonet] problem with subbed slides In-Reply-To: <3b7d8d521002101015t2d08a94cn6e1267670909d7e@mail.gmail.com> References: <3b7d8d521002101015t2d08a94cn6e1267670909d7e@mail.gmail.com> Message-ID: Just for interest's sake could this be some fungal growth? Try staining a slide with H/E and look under the microscope. how to fix? perhaps acid digestion and resubbing? On Wed, Feb 10, 2010 at 8:15 PM, Hisham Mohammed wrote: > dear histonetters > > following gelatin subbing of big glass slides we observed numerous white > patches in almost the entire batch of slides subbed. > > what could be the remedy to remove these white patches. > > waiting for a practical solution. > > with regards > > hisham mohammed > senior research centre > national brain research centre > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From paintedsplashes <@t> yahoo.com Thu Feb 11 03:33:55 2010 From: paintedsplashes <@t> yahoo.com (Jeanne Clark) Date: Thu Feb 11 03:34:00 2010 Subject: [Histonet] Please post on Histonet...... Message-ID: <988528.67513.qm@web30704.mail.mud.yahoo.com> Attention Histotechs: A full time night shift position is available for a ?qualified, licensed HT interested in relocating to the beautiful mountains of western North Carolina in Asheville.? ??Mission Hospital is a leader in the health care industry and we continue to seek enthusiastic and talented individuals to join our team. We value our employees and work hard to create an environment that is supportive, respectful and diverse; we are committed to being the best." If you feel you would like to join our team of exceptional caregivers, please log onto www.missionhospitals.org and fill out an application now! ? Jeanne Clark, HT/MLT (ASCP) Pathology Manager Mission Hospital -St. Joseph Campus 428 Biltmore Avenue Asheville, NC 28801 828-213-5169 office 828-207-3218 pager 423-612-1213 cell ? ? 600 Skyloft Dr. Unit 203 Asheville, NC 28801 423-612-1213 cell 828-213-5169 office ? ? From JCBRITTON <@t> Cheshire-Med.COM Thu Feb 11 07:24:09 2010 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Thu Feb 11 07:24:11 2010 Subject: [Histonet] Xylene Recycler In-Reply-To: <4B729F5C.5D38.00EF.0@swmail.sw.org> References: <4B729F5C.5D38.00EF.0@swmail.sw.org> Message-ID: We resolved this issue by placing a filter/screen in our waste to be recycled carboy. After you clean out the excess lint and Schmutz from the line you should be all set! Welcome! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 603-354-5454 ext.2454 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Wednesday, February 10, 2010 12:58 PM To: histonet@lists.utsouthwestern.edu Cc: Patricia Webster Subject: [Histonet] Xylene Recycler Anyone using the new CBG instrument? Am having some issues with "clogged lines" - which we never had with the older model. Really don't want to change processes - which is what they are asking us to do. Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From BoozerKA <@t> ah.org Thu Feb 11 08:00:52 2010 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Thu Feb 11 08:01:32 2010 Subject: [Histonet] LAMBDA AND KAPPA STAINING PROBLEMS In-Reply-To: References: Message-ID: <4B739D13.4AA8.00C0.0@ah.org> I had the same problem and ended up getting an "Option" reagent to work with a different protocol, in addition to getting new antibody lots for both the Kappa and Lambda, but the new protocol only helped a little bit. The tech said we might have to find other antibodies, that their's is just too sensitive. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org >>> ADESUPO ADESUYI 02/10/2010 17:15 >>> Hi, We are having overstaining problems with both our Kappa and Lambda stains using our recently purchased Benchmark XT. Pls, I will appreciate it, if you guys at histoland could offer some suggestions as to taking care of this problem. Thanking you all for your usual cooperation. Adesuyi A, BS,HT(ASCP), HTL(ASCP) _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Thu Feb 11 08:27:02 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Feb 11 08:25:51 2010 Subject: [Histonet] Peripheral Blood Smears Message-ID: We have had this discussion many times in our institute and would like some outside thoughts. Is there a charge/cpt code that is acceptable for the technical aspect of a peripheral smear? Hematology doesn't stain our slides. They make the smear and we accession, stain and coverslip, etc. But the pathologist is the only one actually interpreting the smear. Thanks. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From mpence <@t> grhs.net Thu Feb 11 08:43:55 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Feb 11 08:44:00 2010 Subject: [Histonet] Peripheral Blood Smears In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D53@is-e2k3.grhs.net> You're a Histology Dept.! Get the peripheral smears out of your dept. Peripheral smears belong in Hematology. Make then make something work. This is what I did. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: Thursday, February 11, 2010 8:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peripheral Blood Smears We have had this discussion many times in our institute and would like some outside thoughts. Is there a charge/cpt code that is acceptable for the technical aspect of a peripheral smear? Hematology doesn't stain our slides. They make the smear and we accession, stain and coverslip, etc. But the pathologist is the only one actually interpreting the smear. Thanks. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Thu Feb 11 08:49:27 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Feb 11 08:49:30 2010 Subject: [Histonet] Peloris processor In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553400D2B6A58@exchangent> Message-ID: <274603.8331.qm@web54206.mail.re2.yahoo.com> We have 2 systems in our lab. We process biopsies (sent to us in B-Fix)?on a 2 hour run and they are beautiful. We use 10% NBF, alcohol, xylene, the routine reagents, nothing special except our biopsies?and are processed in formalin, etc. ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! --- On Wed, 2/10/10, Knutson, Deanne wrote: From: Knutson, Deanne Subject: [Histonet] Peloris processor To: "'histonet@lists.utsouthwestern.edu'" Date: Wednesday, February 10, 2010, 11:16 AM We are also interested in other's experiences with the Peloris processor. We are considering a rapid tissue processor and would like to hear from others who have rapid tissue processors.? What type do you have? And please share your pros and cons.? Thank you very much for your help. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlschneider <@t> gmail.com Thu Feb 11 08:55:46 2010 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Thu Feb 11 08:55:50 2010 Subject: [Histonet] Peripheral Blood Smears In-Reply-To: References: Message-ID: <1085e7001002110655w4c20642bk23c25d0e11e86d47@mail.gmail.com> On Thu, Feb 11, 2010 at 8:27 AM, wrote: > Hematology doesn't stain our > slides. Why not? From flnails <@t> texaschildrens.org Thu Feb 11 08:57:09 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Feb 11 08:57:21 2010 Subject: [Histonet] Peripheral Blood Smears In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3D53@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3D53@is-e2k3.grhs.net> Message-ID: I have been fighting this battle for years and still have not been successful. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, February 11, 2010 8:44 AM To: DKBoyd@chs.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Peripheral Blood Smears You're a Histology Dept.! Get the peripheral smears out of your dept. Peripheral smears belong in Hematology. Make then make something work. This is what I did. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: Thursday, February 11, 2010 8:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peripheral Blood Smears We have had this discussion many times in our institute and would like some outside thoughts. Is there a charge/cpt code that is acceptable for the technical aspect of a peripheral smear? Hematology doesn't stain our slides. They make the smear and we accession, stain and coverslip, etc. But the pathologist is the only one actually interpreting the smear. Thanks. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From DKBoyd <@t> chs.net Thu Feb 11 09:02:08 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Feb 11 09:01:01 2010 Subject: [Histonet] Peripheral Blood Smears In-Reply-To: <1085e7001002110655w4c20642bk23c25d0e11e86d47@mail.gmail.com> Message-ID: I would love to get them out of histology, but the pathologist want us to stain them. They are not happy with the hematology stain. Also the report is generated through Histology when it is for pathologist review. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Daniel Schneider Sent by: histonet-bounces@lists.utsouthwestern.edu 02/11/2010 09:57 AM To DKBoyd@chs.net cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Peripheral Blood Smears On Thu, Feb 11, 2010 at 8:27 AM, wrote: > Hematology doesn't stain our > slides. Why not? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From rachel.elliott <@t> thermofisher.com Thu Feb 11 09:03:21 2010 From: rachel.elliott <@t> thermofisher.com (Elliott, Rachel A.) Date: Thu Feb 11 09:04:02 2010 Subject: [Histonet] Peripheral Blood Smears In-Reply-To: Message-ID: <7B380BF8E6354F4994F20E095D43F171EF20D2C5@USPHO-MXVS01.amer.thermo.com> What stain does your hematology department do? Which one are you running? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: Thursday, February 11, 2010 10:02 AM To: Daniel Schneider Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Peripheral Blood Smears I would love to get them out of histology, but the pathologist want us to stain them. They are not happy with the hematology stain. Also the report is generated through Histology when it is for pathologist review. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Daniel Schneider Sent by: histonet-bounces@lists.utsouthwestern.edu 02/11/2010 09:57 AM To DKBoyd@chs.net cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Peripheral Blood Smears On Thu, Feb 11, 2010 at 8:27 AM, wrote: > Hematology doesn't stain our > slides. Why not? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Feb 11 09:05:32 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Feb 11 09:05:37 2010 Subject: [Histonet] Peripheral Blood Smears In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D54@is-e2k3.grhs.net> Show your Pathologist that by moving the peripheral smears back to Hematology, they will be able to correlate the smear with the rest of the patients blood work. Have your Lab LIS person work with Hematology and build a template to attach the smear report to the rest of the patients blood work. The reason your Pathologist want/have this in histology is because the dictate the smear and the only area that has dictation in the lab is Histology. It is a mind set they have. -----Original Message----- From: Nails, Felton [mailto:flnails@texaschildrens.org] Sent: Thursday, February 11, 2010 8:57 AM To: Mike Pence; DKBoyd@chs.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Peripheral Blood Smears I have been fighting this battle for years and still have not been successful. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, February 11, 2010 8:44 AM To: DKBoyd@chs.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Peripheral Blood Smears You're a Histology Dept.! Get the peripheral smears out of your dept. Peripheral smears belong in Hematology. Make then make something work. This is what I did. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: Thursday, February 11, 2010 8:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peripheral Blood Smears We have had this discussion many times in our institute and would like some outside thoughts. Is there a charge/cpt code that is acceptable for the technical aspect of a peripheral smear? Hematology doesn't stain our slides. They make the smear and we accession, stain and coverslip, etc. But the pathologist is the only one actually interpreting the smear. Thanks. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================================================================== ====== From dlschneider <@t> gmail.com Thu Feb 11 09:08:43 2010 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Thu Feb 11 09:08:48 2010 Subject: [Histonet] Peripheral Blood Smears In-Reply-To: References: <1085e7001002110655w4c20642bk23c25d0e11e86d47@mail.gmail.com> Message-ID: <1085e7001002110708s1840d84frdc8fced258084c8d@mail.gmail.com> It seems odd to me that Histology is producing a better Wright Giemsa than Hematology can. It's their bread and butter. Who is the pathologist over Hematology? Perhaps y'all can have a little pow-wow, and help Hematology improve their stain. (Is it that your pathologists want a manual stain and hematology is using an automated stainer and the docs don't like the way it looks? Automated stainers can be tweaked. Besides, hematology should be able to do manual stains.) On Thu, Feb 11, 2010 at 9:02 AM, wrote: > > I would love to get them out of histology, but the pathologist want us to > stain them. They are not happy with the hematology stain. Also the report > is generated through Histology when it is for pathologist review. > > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: > 804-765-5582 l dkboyd@chs.net > > > > > > > *Daniel Schneider * > Sent by: histonet-bounces@lists.utsouthwestern.edu > > 02/11/2010 09:57 AM > To > DKBoyd@chs.net > cc > histonet@lists.utsouthwestern.edu > Subject > Re: [Histonet] Peripheral Blood Smears > > > > > On Thu, Feb 11, 2010 at 8:27 AM, wrote: > > > Hematology doesn't stain our > > slides. > > > Why not? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please notify > the sender immediately and delete the material from your computer. Do not > deliver, distribute or copy this message and do not disclose its contents or > take any action in reliance on the information it contains. From DKBoyd <@t> chs.net Thu Feb 11 09:24:48 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Feb 11 09:23:39 2010 Subject: [Histonet] Peripheral Blood Smears In-Reply-To: <7B380BF8E6354F4994F20E095D43F171EF20D2C5@USPHO-MXVS01.amer.thermo.com> Message-ID: We are running a Romanowsky stain which is the same as our hematology department (same stain, same reagents and stainer). Perhaps I should mention, we also assist with and stain all bone marrows as well. We are a relatively small hospital (260 beds) we have 3 pathologist and everyone has a say in what goes down. All three perfer that we do the staining, assisting etc. Of course, that could be a compliment to our department. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Elliott, Rachel A." Sent by: histonet-bounces@lists.utsouthwestern.edu 02/11/2010 10:05 AM To "DKBoyd@chs.net" , Daniel Schneider cc "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Subject RE: [Histonet] Peripheral Blood Smears What stain does your hematology department do? Which one are you running? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: Thursday, February 11, 2010 10:02 AM To: Daniel Schneider Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Peripheral Blood Smears I would love to get them out of histology, but the pathologist want us to stain them. They are not happy with the hematology stain. Also the report is generated through Histology when it is for pathologist review. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Daniel Schneider Sent by: histonet-bounces@lists.utsouthwestern.edu 02/11/2010 09:57 AM To DKBoyd@chs.net cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Peripheral Blood Smears On Thu, Feb 11, 2010 at 8:27 AM, wrote: > Hematology doesn't stain our > slides. Why not? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Johnathon.Myers <@t> USOncology.com Thu Feb 11 09:44:29 2010 From: Johnathon.Myers <@t> USOncology.com (Myers, Johnathon) Date: Thu Feb 11 09:44:35 2010 Subject: [Histonet] LIS Message-ID: Can anyone recommend a lab information system that would function well in a histology lab? Thanks, John The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only. Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From DKBoyd <@t> chs.net Thu Feb 11 10:02:00 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Feb 11 10:00:52 2010 Subject: [Histonet] LIS In-Reply-To: Message-ID: We use Impac (PowerPath). It is great for histology/cytology. It has great QC potential. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Myers, Johnathon" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/11/2010 10:46 AM To cc Subject [Histonet] LIS Can anyone recommend a lab information system that would function well in a histology lab? Thanks, John The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only. Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From mpence <@t> grhs.net Thu Feb 11 10:28:16 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Feb 11 10:28:19 2010 Subject: [Histonet] LIS In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D56@is-e2k3.grhs.net> CoPath Plus is the Gold Standard as far as I am concerned. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Myers, Johnathon Sent: Thursday, February 11, 2010 9:44 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS Can anyone recommend a lab information system that would function well in a histology lab? Thanks, John The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only. Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mturner <@t> carisdx.com Thu Feb 11 10:46:38 2010 From: mturner <@t> carisdx.com (Turner, Mark) Date: Thu Feb 11 10:46:45 2010 Subject: [Histonet] Microwave Validation & Certification Message-ID: Does anyone know of companies which do testing and validation of microwaves in the lab? Mark Turner, HT(ASCP) QIHC Supervisor IHC Target Now MPI Caris Life Sciences 445 N. 5th Street Phoenix, AZ 85004 Cell: 602-309-5084 Direct 602-358-8913 Fax: 602-358-8919 mturner@carisdx.com From relia1 <@t> earthlink.net Thu Feb 11 10:52:33 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Feb 11 10:52:42 2010 Subject: [Histonet] RELIA Solutions Histology Jobs Alert 2/11/2010 Message-ID: Hi Histonetters!! I hope everybody is staying warm. When you get a chance take a break get a cup of cocoa and please take a look at my current openings. Here are the current openings: HISTOLOGY/PATHOLOGY MANAGEMENT NY- Orange/Rockland County Histology Supervisor MA - Cape Cod Pathology Supervisor OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA - Central CA - Pathology Supervisor CA - Los Angeles -Histology Supervisor HISTOTECHS FL - Miami- Histotech growing private lab GA _- Grossing Histotechnologist night shift MD - Histotech - Hospital NY-Orange/Rockand County-Brand New Lab NYS license req PATHOLOGIST'S ASSISTANTS NC - Charlotte PA grad from NAACLES program required MA - Boston Hospital environment FL - Miami - growing private lab MARKETING REP - HISTO/CYTO products Pacific NW region All of my clients provide excellent benefits a competitive salary and relocation assistance. For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542 Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net < mailto:relia1@earthlink.net> << http://home.earthlink.net/~relia1>> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Reuel.Cornelia <@t> tsrh.org Thu Feb 11 11:05:08 2010 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu Feb 11 11:06:06 2010 Subject: [Histonet] Slide for bone tissue Message-ID: <4B73E464020000C50006FCCA@mail.TSRH.ORG> Dear histonetters, I need to know an available slides for bone tissue especcally with cartillage. I have done a positively charged slides and did a subbed slides but it fails on our special staining and immunohistochem. Please help us. Does APES slides will help resolved this problem? Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From JWeems <@t> sjha.org Thu Feb 11 11:08:05 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Feb 11 11:08:02 2010 Subject: [Histonet] LIS In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3D56@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3D56@is-e2k3.grhs.net> Message-ID: <2A606F30FD3EF848831D7B765726CDFDF4E4@ITSSSXM01V5.one.ads.che.org> I just heard of a new web based system http://www.webpathlab.com/ that you might want to check out. Not sure how it works with workload and management reports, etc. but a pathologist friend of mine uses in her private lab and is very satisfied with it for the reporting and billing side. Best, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, February 11, 2010 11:28 To: Myers, Johnathon; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] LIS CoPath Plus is the Gold Standard as far as I am concerned. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Myers, Johnathon Sent: Thursday, February 11, 2010 9:44 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS Can anyone recommend a lab information system that would function well in a histology lab? Thanks, John The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only. Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From POWELL_SA <@t> mercer.edu Thu Feb 11 11:42:20 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Feb 11 11:42:27 2010 Subject: [Histonet] Slide for bone tissue In-Reply-To: <4B73E464020000C50006FCCA@mail.TSRH.ORG> References: <4B73E464020000C50006FCCA@mail.TSRH.ORG> Message-ID: <9BF995BC0E47744E9673A41486E24EE2242A1816CD@MERCERMAIL.MercerU.local> When I was doing immunos, for difficult tissues like bone and bloody specimens, I used Sta-On from Surgipath and regular frosted slides. I had problems with the charged/subbed slides washing as well. Sta-On did the trick. I now use it for my autopsies that I cut for the GBI/Medical Examiner and it works well. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia Sent: Thursday, February 11, 2010 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide for bone tissue Dear histonetters, I need to know an available slides for bone tissue especcally with cartillage. I have done a positively charged slides and did a subbed slides but it fails on our special staining and immunohistochem. Please help us. Does APES slides will help resolved this problem? Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ogv_histech <@t> yahoo.com Thu Feb 11 11:56:33 2010 From: ogv_histech <@t> yahoo.com (oscar gonzalez) Date: Thu Feb 11 11:56:38 2010 Subject: [Histonet] Imaging protocol Message-ID: <891450.40500.qm@web53506.mail.re2.yahoo.com> Does anyone have a protocol/procedure to validate imaging analysis? From tanaforjesus <@t> yahoo.com Thu Feb 11 12:22:19 2010 From: tanaforjesus <@t> yahoo.com (Tana Badalamenti) Date: Thu Feb 11 12:22:23 2010 Subject: [Histonet] static Message-ID: <579204.6664.qm@web111416.mail.gq1.yahoo.com> Pathologist say that they SEE static on H@E slides. Does anyone know what that is and how to remedy it? Thanks, Tana Southern Pathology, Covington, La. From liz <@t> premierlab.com Thu Feb 11 12:51:26 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Feb 11 12:51:40 2010 Subject: [Histonet] static In-Reply-To: <579204.6664.qm@web111416.mail.gq1.yahoo.com> Message-ID: Why don't you ask them to sit down at scope and show you what they are seeing? That's the best way to approach any staining or sectioning issues. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tana Badalamenti Sent: Thursday, February 11, 2010 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] static Pathologist say that they SEE static on H@E slides. Does anyone know what that is and how to remedy it? Thanks, Tana Southern Pathology, Covington, La. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Fawn.Bomar <@t> HalifaxRegional.com Thu Feb 11 13:06:56 2010 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Thu Feb 11 13:07:16 2010 Subject: [Histonet] Help... Message-ID: Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From liz <@t> premierlab.com Thu Feb 11 13:28:27 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Feb 11 13:28:30 2010 Subject: [Histonet] Help... In-Reply-To: Message-ID: Fawn CAP has specific quidelines, its right there in the questions, did you do what that question is asking and have you documented what was done. They tell you right in the check sheet what needs to be completed. Test validation must be performed on a minimum of 25 cases (they recommend 25- 100) and they tell you how to validate too. There is also an article that you can get on the CAP website on IHC standardization. I have a pdf of it here if you need it. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Thursday, February 11, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help... Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Feb 11 13:41:00 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Feb 11 13:41:10 2010 Subject: [Histonet] KRAS/EGFR testing Message-ID: <4B7416FB.7400.0077.1@harthosp.org> Does anyone know of a lab that does KRAS/EGFR molecular testing on alcohol-fixed cytology smears for patient care? Thanks! Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From Wanda.Smith <@t> HCAhealthcare.com Thu Feb 11 13:50:37 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Thu Feb 11 13:50:37 2010 Subject: [Histonet] KRAS/EGFR testing In-Reply-To: <4B7416FB.7400.0077.1@harthosp.org> References: <4B7416FB.7400.0077.1@harthosp.org> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1384DA12DD@NADCWPMSGCMS03.hca.corpad.net> You may want to check with Genzyme Genetics Lab with this question. My rep is Elissa Quinn ph #919-793-3300. Genzyme is where we send out KRAS and EGFR tissue block specimens . Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 11, 2010 2:41 PM To: Histonet Subject: [Histonet] KRAS/EGFR testing Does anyone know of a lab that does KRAS/EGFR molecular testing on alcohol-fixed cytology smears for patient care? Thanks! Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Feb 11 14:00:51 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Feb 11 14:00:54 2010 Subject: [Histonet] KRAS/EGFR testing In-Reply-To: <4B7416FB.7400.0077.1@harthosp.org> References: <4B7416FB.7400.0077.1@harthosp.org> Message-ID: <2A606F30FD3EF848831D7B765726CDFDF51A@ITSSSXM01V5.one.ads.che.org> Try Clarient - 888-443-3311. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 11, 2010 14:41 To: Histonet Subject: [Histonet] KRAS/EGFR testing Does anyone know of a lab that does KRAS/EGFR molecular testing on alcohol-fixed cytology smears for patient care? Thanks! Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Thu Feb 11 15:13:38 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 11 15:13:43 2010 Subject: [Histonet] static In-Reply-To: <579204.6664.qm@web111416.mail.gq1.yahoo.com> Message-ID: <74022.6535.qm@web65707.mail.ac4.yahoo.com> Either your pathologist is pulling your leg of s/he does not know what s/he is talking about. "Static", as in "electrostatic" energy, is just that, a manifestation of energy INVISIBLE to the human eye in itself althoug you can see its effects in how some objects behave. Let your pathologist SHOW you that "visible static" energy, and let me know. It is always interesting to learn something new, although I am sure your will "see" nothing at all! Ren? J. --- On Thu, 2/11/10, Tana Badalamenti wrote: From: Tana Badalamenti Subject: [Histonet] static To: histonet@lists.utsouthwestern.edu Date: Thursday, February 11, 2010, 1:22 PM Pathologist say that they SEE static on H@E slides. Does anyone know what that is and how to remedy it? Thanks, Tana Southern Pathology, Covington, La. ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 11 15:14:44 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 11 15:14:47 2010 Subject: [Histonet] Help... In-Reply-To: Message-ID: <549744.9350.qm@web65706.mail.ac4.yahoo.com> And what did you do? Ren? J. --- On Thu, 2/11/10, Fawn Bomar wrote: From: Fawn Bomar Subject: [Histonet] Help... To: "histonet@lists.utsouthwestern.edu" Date: Thursday, February 11, 2010, 2:06 PM Does anyone have a protocol or procedure for validating the Her2 antibody?? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged.? It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKnutson <@t> primecare.org Thu Feb 11 16:14:52 2010 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Thu Feb 11 16:15:48 2010 Subject: [Histonet] Cryostats Message-ID: <4F0B7161A6CD524FAD8017D52E1553400D2B6A6E@exchangent> Dear Histonetters, Does anyone know if there is some type of UV light kit that is available to be put into older model cryostats that would help meet the CAP standard for disinfecting weekly? Would appreciate hearing if there is! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org From lscott <@t> sfcn.org Fri Feb 12 07:27:45 2010 From: lscott <@t> sfcn.org (Scott Hendricksen) Date: Fri Feb 12 07:27:54 2010 Subject: [Histonet] Does anyone use Rainbow Frost slides from IMEB ? Message-ID: <56673E24-AF24-44BB-A77A-CE8DB336906A@sfcn.org> Hi, We have been looking for a better, cleaner slide to use in our lab. We tried some samples from IMEB out in California that were cleaner than the Erie slides we have been using forever. Does anyone use the Rainbow Frost slides ? Any suggestions on quality slides? Also what is the typical price per gross for good, clean, consistent slides? These are on sale for $7.20 per gross. Thanks for all of the suggestions in advance, Scott Hendricksen HT(ASCP) From jaylundgren <@t> gmail.com Fri Feb 12 09:25:29 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Feb 12 09:25:37 2010 Subject: [Histonet] Distance learning? Message-ID: A few years ago I remember hearing about a NAACLS approved, distance learning Histotechnology program. They were using the Internet and teleconferencing to train students. Is anyone still doing this, and could you tell me where? Thanks for your time, Jay A. Lundgren, M.S., HTL (ASCP) From JWeems <@t> sjha.org Fri Feb 12 09:46:02 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Feb 12 09:44:44 2010 Subject: [Histonet] Distance learning? In-Reply-To: References: Message-ID: <2A606F30FD3EF848831D7B765726CDFDF5AE@ITSSSXM01V5.one.ads.che.org> Darton College in Albany GA has an Associates Degree program. http://www.study-online.net/schools/darton-college/13439.htm Contact: Carl Sagasser, BS, HT(ASCP) Educational Coordinator Histotechnology Program Darton College 2400 Gillionville Road Albany, GA 31701 P: (229) 317-6974 F: (229) 317-6682 There is also a program at Indiana University - http://www.indiana.edu/~bltindy/ahlt/histotech/cert.html Glenda Hood Hoye developed that program but she is no longer there. The web site above will give you some contact numbers if you want to check on it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Friday, February 12, 2010 10:25 To: histonet Subject: [Histonet] Distance learning? A few years ago I remember hearing about a NAACLS approved, distance learning Histotechnology program. They were using the Internet and teleconferencing to train students. Is anyone still doing this, and could you tell me where? Thanks for your time, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From flnails <@t> texaschildrens.org Fri Feb 12 09:52:03 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Feb 12 09:52:21 2010 Subject: [Histonet] Ultralight In-Reply-To: <2A606F30FD3EF848831D7B765726CDFDF5AE@ITSSSXM01V5.one.ads.che.org> References: <2A606F30FD3EF848831D7B765726CDFDF5AE@ITSSSXM01V5.one.ads.che.org> Message-ID: Have anyone in Histoland converted from 10% NBF to the Ultralight Histology system of processing? I think it is marketed by Pacific Southwest Lab Equipment -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, February 12, 2010 9:46 AM To: Jay Lundgren; histonet Subject: RE: [Histonet] Distance learning? Darton College in Albany GA has an Associates Degree program. http://www.study-online.net/schools/darton-college/13439.htm Contact: Carl Sagasser, BS, HT(ASCP) Educational Coordinator Histotechnology Program Darton College 2400 Gillionville Road Albany, GA 31701 P: (229) 317-6974 F: (229) 317-6682 There is also a program at Indiana University - http://www.indiana.edu/~bltindy/ahlt/histotech/cert.html Glenda Hood Hoye developed that program but she is no longer there. The web site above will give you some contact numbers if you want to check on it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Friday, February 12, 2010 10:25 To: histonet Subject: [Histonet] Distance learning? A few years ago I remember hearing about a NAACLS approved, distance learning Histotechnology program. They were using the Internet and teleconferencing to train students. Is anyone still doing this, and could you tell me where? Thanks for your time, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From mpence <@t> grhs.net Fri Feb 12 09:53:18 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Feb 12 09:53:35 2010 Subject: [Histonet] fixation times for breast cases Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D5C@is-e2k3.grhs.net> Happy Friday to All: I have started seeing things written that states that the time of fixation in 10% neutral buffered formalin will be changing to 72 hrs for breast cases and this will include not only HER2,but also ER/PR guidelines and these should be releases in 2010. Just wondering if anyone has any insight of when this may be coming. Having techs come in on the weekend is starting to get to be a problem and this 72 hour change would make everything better! Here is one of the articles I have seen. http://cme.medscape.com/viewarticle/713825 Just wondering if anyone knows, Mike ANP.22998 Phase I N/A YES NO If the laboratory assesses HER2 protein over-expression by immunohistochemistry (IHC) or HER2 gene amplification by fluorescence in situ hybridization (FISH), does the laboratory have a documented procedure for ensuring appropriate length of fixation of specimens tested? NOTE: Specimens subject to HER2 testing should be fixed in 10% neutral buffered formalin for at least 6 hours and no longer than 48 hours. While core biopsies must not be fixed for less than 1 hour, it is recommended that such specimens have the same fixation as larger specimens (i.e., 6 hours minimum). It is recommended that time of fixation be recorded and included in the report if available. While the maximum fixation time of 48 hours is not an exclusion criterion for HER2 testing, laboratories should qualify any negative results for specimens fixed longer than 48 hours. For cases with negative results by IHC, consideration should be given to performing confirmatory analysis by FISH. Laboratories testing specimens obtained from another institution should have a policy that addresses time of fixation. Information on time of fixation may be obtained by appropriate questions on the laboratory's requisition form. The time of fixation should be recorded in the final report. From nursekarla <@t> comcast.net Fri Feb 12 10:21:39 2010 From: nursekarla <@t> comcast.net (Karla Szeszol) Date: Fri Feb 12 10:21:52 2010 Subject: [Histonet] (no subject) Message-ID: Hello Histoworld, I am a histotechnology student and I was wondering if someone would please help me. As part of our program it is required that we become familiar with the process laboratories go through to become CAP accredited. As part of our Policy and Procedure class, I am trying to find information on the topics of "Legal" and "Correlation". Which CAP questions apply to these topics? What are the processes laboratories use to satisfy the CAP questions for accreditation. It would help if I had some examples how different kinds of facilities (research, hospital and industry) comply with CAP requirements. Thank you for your help. See you in Histoworld. Sincerely, Karla Bradshaw nursekarla@comcast.net 847-669=5044 home 847-207=6945 cell From rjbuesa <@t> yahoo.com Fri Feb 12 10:38:34 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 12 10:38:39 2010 Subject: [Histonet] Distance learning? In-Reply-To: Message-ID: <670263.73997.qm@web65715.mail.ac4.yahoo.com> Indiana University Ren? K. --- On Fri, 2/12/10, Jay Lundgren wrote: From: Jay Lundgren Subject: [Histonet] Distance learning? To: "histonet" Date: Friday, February 12, 2010, 10:25 AM ? A few years ago I remember hearing about a NAACLS approved, distance learning Histotechnology program.? They were using the Internet and teleconferencing to train students.? Is anyone still doing this, and could you tell me where? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???Thanks for your time, ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Fri Feb 12 11:32:51 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Fri Feb 12 11:33:20 2010 Subject: [Histonet] Hypothetical question Message-ID: Out of curiosity, how many Histotechs would like to work in a lab where you have an average of 100-150 blocks per day, a hand full of special stains at most which are done on the Dako artisan, IHC done on the Ventana XT and less than a hand full of cytology preps. You might have 0-5 frozen sections per day as well . You would even have help from one or two other technologist. You could work 4/10 hour days or 5/8 hour days. Do any of you think this sounds like too much work? Maybe this hypothetical lab is located in the beautiful area of Pensacola Fl? All of this is Hypothetical... <<<< Again, I'm just curious :-) Thanks Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From Maxim_71 <@t> mail.ru Fri Feb 12 11:34:04 2010 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Fri Feb 12 11:34:09 2010 Subject: [Histonet] Antibodies for rabbit tussues Message-ID: <76456560.20100212203404@mail.ru> Dear Hisonetters! I need in some advise. One of colleagues of mine asked: We need to study CD3, CD4, CD8, CD20, CD34, CD68 and Collagen IV IHC-markers on the rabbit tissues. We tried poly Dako ab's with EnVision system with high background staining. Histophine (mouse) detection system was not react. Please, anyone help for us which antibodies, detection system, manufacturers and vendors need to use? Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From NLinke <@t> mednet.ucla.edu Fri Feb 12 11:46:16 2010 From: NLinke <@t> mednet.ucla.edu (Linke, Noelle) Date: Fri Feb 12 11:46:23 2010 Subject: [Histonet] Hypothetical question In-Reply-To: References: Message-ID: <0C96F0BFE078D74C91A1C541D24A6AE4011ABE664C@EMGMB1.ad.medctr.ucla.edu> Hahahaha....cranky staff?? I'm not a big fan of Florida....too muggy, makes my hair crazy. No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services UCLA Department of Pathology and Laboratory Medicine Phone: 310-825-7397 Fax: 310-983-3289 nlinke@mednet.ucla.edu Pager: 97471 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Friday, February 12, 2010 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hypothetical question Out of curiosity, how many Histotechs would like to work in a lab where you have an average of 100-150 blocks per day, a hand full of special stains at most which are done on the Dako artisan, IHC done on the Ventana XT and less than a hand full of cytology preps. You might have 0-5 frozen sections per day as well . You would even have help from one or two other technologist. You could work 4/10 hour days or 5/8 hour days. Do any of you think this sounds like too much work? Maybe this hypothetical lab is located in the beautiful area of Pensacola Fl? All of this is Hypothetical... <<<< Again, I'm just curious :-) Thanks Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From DKnutson <@t> primecare.org Fri Feb 12 11:53:00 2010 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Fri Feb 12 11:53:42 2010 Subject: [Histonet] UV light in cryostats Message-ID: <4F0B7161A6CD524FAD8017D52E1553400D2B6A7B@exchangent> Hello Everyone, Does anyone know if the UV lighting that can now be purchased with new cryostats will fulfill the CAP disinfection standard? Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknutson@primecare.org From histosearch <@t> gmail.com Fri Feb 12 11:57:11 2010 From: histosearch <@t> gmail.com (HistoLab) Date: Fri Feb 12 11:57:18 2010 Subject: [Histonet] Does anyone use Rainbow Frost slides from IMEB ? Message-ID: <521c6d261002120957i53e63da3q284182c60925d9e9@mail.gmail.com> Scott, I would be happy to send you samples of our colored end slides or charged slides. Please let me know if you are interested. Regards, Matt Gorilla Scientific 1-866-435-4977 www.GorillaScientific.com Hi, We have been looking for a better, cleaner slide to use in our lab. We tried some samples from IMEB out in California that were cleaner than the Erie slides we have been using forever. Does anyone use the Rainbow Frost slides ? Any suggestions on quality slides? Also what is the typical price per gross for good, clean, consistent slides? These are on sale for $7.20 per gross. Thanks for all of the suggestions in advance, Scott Hendricksen HT(ASCP) From KPercival <@t> wyeth.com Fri Feb 12 12:00:07 2010 From: KPercival <@t> wyeth.com (Percival Karen) Date: Fri Feb 12 12:00:20 2010 Subject: [Histonet] Does anyone use Rainbow Frost slides from IMEB ? In-Reply-To: <521c6d261002120957i53e63da3q284182c60925d9e9@mail.gmail.com> References: <521c6d261002120957i53e63da3q284182c60925d9e9@mail.gmail.com> Message-ID: <4B7550D702000011001F89DF@gv01a67m.gv.us.pri.wyeth.com> On that note, we don't have a problem with clean slides, but the coverslips are dusty as can be. Any suggestions out there? Karen >>> HistoLab 2/12/2010 12:57 PM >>> Scott, I would be happy to send you samples of our colored end slides or charged slides. Please let me know if you are interested. Regards, Matt Gorilla Scientific 1-866-435-4977 www.GorillaScientific.com Hi, We have been looking for a better, cleaner slide to use in our lab. We tried some samples from IMEB out in California that were cleaner than the Erie slides we have been using forever. Does anyone use the Rainbow Frost slides ? Any suggestions on quality slides? Also what is the typical price per gross for good, clean, consistent slides? These are on sale for $7.20 per gross. Thanks for all of the suggestions in advance, Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From estellamireles <@t> gmail.com Fri Feb 12 12:03:43 2010 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Fri Feb 12 12:03:50 2010 Subject: [Histonet] FT Job in Houston (Near Bush Airport) Message-ID: We have a FT job opening for Hermann Hospital Northeast. Routine Histo work. Heavy bx's. Weekends off. Please contact the Hermann Hospital Website. Need more info - 281 540 6275 Thanks Stella From sweething63 <@t> msn.com Fri Feb 12 13:11:35 2010 From: sweething63 <@t> msn.com (R J VAZQUEZ) Date: Fri Feb 12 13:11:40 2010 Subject: [Histonet] Does anyone use Rainbow Frost slides from IMEB ? In-Reply-To: <521c6d261002120957i53e63da3q284182c60925d9e9@mail.gmail.com> References: <521c6d261002120957i53e63da3q284182c60925d9e9@mail.gmail.com> Message-ID: Scott, Rex Johnson of Creative Waste has a great deal on rainbow colored end slides, with a vast section to choose from. His phone number is 1(888)795-8300. He is based out of Portland Oregon, but I am sure he can send them to you. Robyn > Date: Fri, 12 Feb 2010 12:57:11 -0500 > From: histosearch@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Does anyone use Rainbow Frost slides from IMEB ? > > Scott, > > I would be happy to send you samples of our colored end slides or > charged slides. Please let me know if you are interested. > > Regards, > > Matt > Gorilla Scientific > 1-866-435-4977 > www.GorillaScientific.com > > Hi, > > We have been looking for a better, cleaner slide to use in our lab. > We tried some samples from IMEB out in California that were cleaner > than the Erie slides we have been using forever. Does anyone use the > Rainbow Frost slides ? Any suggestions on quality slides? Also what is > the typical price per gross for good, clean, consistent slides? These > are on sale for $7.20 per gross. > > Thanks for all of the suggestions in advance, > > Scott Hendricksen HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Fri Feb 12 13:51:50 2010 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Feb 12 13:56:54 2010 Subject: [Histonet] Re: Does anyone use Rainbow Frost slides from IMEB Message-ID: <11D9615B89C10747B1C985966A63D7CA2C5C88B1F6@KCL-MAIL04.kclad.ds.kcl.ac.uk> I may have missed your explanatory post so, my apologies. How do you know that your slides are "dirty" ? Thanks, carl From Janet.Bonner <@t> FLHOSP.ORG Fri Feb 12 15:00:21 2010 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Feb 12 15:01:01 2010 Subject: [Histonet] Hypothetical question References: <0C96F0BFE078D74C91A1C541D24A6AE4011ABE664C@EMGMB1.ad.medctr.ucla.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2CC5@fhosxchmb006.ADVENTISTCORP.NET> Uh....Not today. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Linke, Noelle Sent: Fri 2/12/2010 12:46 PM To: Kim.Donadio@bhcpns.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Hypothetical question Hahahaha....cranky staff?? I'm not a big fan of Florida....too muggy, makes my hair crazy. No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services UCLA Department of Pathology and Laboratory Medicine Phone: 310-825-7397 Fax: 310-983-3289 nlinke@mednet.ucla.edu Pager: 97471 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Friday, February 12, 2010 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hypothetical question Out of curiosity, how many Histotechs would like to work in a lab where you have an average of 100-150 blocks per day, a hand full of special stains at most which are done on the Dako artisan, IHC done on the Ventana XT and less than a hand full of cytology preps. You might have 0-5 frozen sections per day as well . You would even have help from one or two other technologist. You could work 4/10 hour days or 5/8 hour days. Do any of you think this sounds like too much work? Maybe this hypothetical lab is located in the beautiful area of Pensacola Fl? All of this is Hypothetical... <<<< Again, I'm just curious :-) Thanks Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From jfi786 <@t> aol.com Fri Feb 12 19:22:01 2010 From: jfi786 <@t> aol.com (jfi786@aol.com) Date: Fri Feb 12 19:22:22 2010 Subject: [Histonet] QIHC Message-ID: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj From rjbuesa <@t> yahoo.com Sat Feb 13 09:21:28 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 13 09:21:33 2010 Subject: [Histonet] QIHC In-Reply-To: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> Message-ID: <253066.94334.qm@web65702.mail.ac4.yahoo.com> Contact the NSH because I am sure they will be able to help you. Ren? J. --- On Fri, 2/12/10, jfi786@aol.com wrote: From: jfi786@aol.com Subject: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu Date: Friday, February 12, 2010, 8:22 PM Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Feb 13 17:01:28 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Feb 13 17:02:08 2010 Subject: SPAM-LOW: [Histonet] QIHC In-Reply-To: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> References: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> Message-ID: <90CDE91BC9404B16A1967D9FB61CD218@prueggihctechlt> If you are a member of NSH you can join the NSH IHC Resource Group online at www.ihcrg.org , we have references list on our website for taking the QIHC and presentations to help with that as well. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jfi786@aol.com Sent: Friday, February 12, 2010 6:22 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] QIHC Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdwyer3322 <@t> aol.com Sun Feb 14 11:05:45 2010 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Feb 14 11:06:14 2010 Subject: [Histonet] Texas Society for Histotechnology District I Message-ID: <8CC7BAF15210205-F2C-14768@webmail-d086.sysops.aol.com> Histonetters: The Texas Society for Histotechnology District I, DFW area, is planning a day of workshops on March 6, 2010 from 8:00am- 4:00pm. If you are interested in attending please see contact information below: DISTRICT I MEETING SATURDAY, MARCH 6, 2010 8:00-4:00 Immunohistochemical Studies in Cytologic Diagnosis Presented By :Dr. Carol Adair, Fran Hamlin And Mau Tran, Baylor Medical Center Dallas , Tx Instrument and Method Validation Presented By: Kathleen Dwyer, Ameripath Laboratories, Dallas, Tx And Debbie Sienna, Ventana Medical Systems, Tucson, AZ Antibody Production and Detection Chemistry Presented By: Traci DeGeer, Ventana Medical Systems, Tucson, Az LOCATION: CARIS DIAGNOSTICS 5566 MacArthur Blvd. Irving, Tx New Location of Caris Diagnostics BREAKFAST PROVIDED BY: SAKURA FINETEK, Mack McKinney LUNCH PROVIDED BY: VENTANA MEDICAL SYSTEMS, John Hull FREE CEU CREDITS RSVP By: March 4th via e mail to Matt Shertzer mshertzer@carisdx.com, Questions Please call Meredith Hale 214-596-2219 Or Brenda Brummell 214-596-2271 From donh7 <@t> earthlink.net Mon Feb 15 00:02:27 2010 From: donh7 <@t> earthlink.net (Don Hammer) Date: Mon Feb 15 00:03:01 2010 Subject: [Histonet] Hi to all............. Message-ID: <281414D805204848BA1CC3D07AB02D96@Don> Hi Histology Professionals, I just finished reading the recent issue of "NSH In Action", noting a reference to "Histonet" so thought to drop in to see how this very important, long lasting exchange of information is going. Congrats Linda with thanks to the UTSouthwestern for supporting the site. (14 years now I believe) Perhaps there are a few colleagues still here, continuing to offer assistance from their wealth of experience and knowledge, from back in my Laboratory days (or was that Daze?) :>) It's been near 12 years since retiring, wouldn't give it up for anything, but often think of you all who are in the Profession and now preparing for "Histotechnology Professionals Day" Such a great opportunity to blow your own horn a bit while making the general public aware of your support in the various facets of Medicine. Don't forget to celebrate all you do on a daily basis. I'm completely blown away at how NSH has grown from our Founding Meeting 36 years ago with it's extensive Symposium/Convention, Educational Materials, Journal of Histotechnology, Summer Symposium's, Teleconferences, Specialty Forums, Awards, Positions with or on Boards of National Organizations, just to highlight a few. When viewing the Website containing so much info for Members it's hard to believe much of it was kept on Card files and File drawers manned by one person in the Office. What's difficult to understand is the total Membership is nearly the same number. Well just sending some thoughts. Think I figured out how to post a comment, now I need to go figure out how to view the daily posts on "Histonet" It's been so long since doing that. Don Hammer, Retired Guy " I can't remember the last time I wasn't at least kind of tired." "I totally take back all those times I didn't want to nap when I was younger" From JPeters <@t> bostwicklaboratories.com Mon Feb 15 07:24:41 2010 From: JPeters <@t> bostwicklaboratories.com (Justin Peters) Date: Mon Feb 15 07:24:46 2010 Subject: [Histonet] IHC TBS wash buffer Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F0ECA0BC3@mail1.BOSTWICK.COM> Can anyone tell me what an acceptable pH range for IHC TBS wash buffer is? From jsantiago <@t> bellsouth.net Mon Feb 15 07:29:08 2010 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Mon Feb 15 07:29:12 2010 Subject: [Histonet] Florida Society for Histotechnology-Region III Meeting Message-ID: <967631.97107.qm@web180415.mail.gq1.yahoo.com> Dear Histonetters, The program and online registration for the Florida Society for Histotechnology Meeting is now available at www.fshgroup.org. You can also go directly to the online registration process by visiting www.fshmeeting.com. Sincerely, Jerry Santiago, BS, HTL(ASCP)QIHC NSH Region III Director Shands Jacksonville T: 904-244-6149 E-mail: santij1@bellsouth.net From WBENTON <@t> umm.edu Mon Feb 15 08:13:14 2010 From: WBENTON <@t> umm.edu (Walter Benton) Date: Mon Feb 15 08:11:43 2010 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: <4B790FC5.D886.00F4.3@umm.edu> I recently took and passed the examination . I used the Dako book, material from Ethel Macrea's presentation, NSH study guide and material from the other references on the list. Ethel can be reached at emacrea@swskinpath.com She is a wonderful resource. Message: 6 Date: Fri, 12 Feb 2010 20:22:01 -0500 From: jfi786@aol.com Subject: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu Message-ID: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From Fawn.Bomar <@t> HalifaxRegional.com Mon Feb 15 08:24:26 2010 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Mon Feb 15 08:29:01 2010 Subject: [Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17 In-Reply-To: <208330218E165F45AB6307B787F5EA4761CD14115F@RAD-VMSRVEXV2.rad.wustl.edu> References: <208330218E165F45AB6307B787F5EA4761CD14115F@RAD-VMSRVEXV2.rad.wustl.edu> Message-ID: Hi everyone, Since I received this e-mail from Lynne about a question that I asked, I decided it was probably in my best interest not to ask any more questions in general. Thank you to everyone that helped verify that my validation process was indeed up to par! I will stay on the histonet in hopes that I can help others but I will keep my own questions to myself Fawn ________________________________________ From: Jones, Lynne [jonesly@mir.wustl.edu] Sent: Friday, February 12, 2010 5:48 PM To: Fawn Bomar Subject: FYI - Histonet Digest, Vol 75, Issue 17 Hello - I was curious about the facility where you worked, so I looked up Halifax Regional, but it seems congratulations are in order, since Gwen Wade just posted a Press release indicating that the lab passed CAP accreditation. (Though I'm not sure your inquiry about a fundamental process in a histology lab would inspire confidence.) HistoNet is a fabulous resource, but there are some questions you may want to ask using a less traceable e-mail account. HistoNet archives are readily accessible, even to the general public. I do understand the panic and second-guessing that hits right before the inspectors appear, but you obviously had all the I's dotted and t's crossed. The next time will be easier. :) Take care, Lynne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet- ------------------------------ Message: 3 Date: Thu, 11 Feb 2010 14:06:56 -0500 From: "Fawn Bomar" Subject: [Histonet] Help... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 4 Date: Thu, 11 Feb 2010 12:28:27 -0700 From: "Liz Chlipala" Subject: RE: [Histonet] Help... To: "Fawn Bomar" , Message-ID: Content-Type: text/plain; charset="us-ascii" Fawn CAP has specific quidelines, its right there in the questions, did you do what that question is asking and have you documented what was done. They tell you right in the check sheet what needs to be completed. Test validation must be performed on a minimum of 25 cases (they recommend 25- 100) and they tell you how to validate too. There is also an article that you can get on the CAP website on IHC standardization. I have a pdf of it here if you need it. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Thursday, February 11, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help... Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 17 **************************************** The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From Fawn.Bomar <@t> HalifaxRegional.com Mon Feb 15 08:29:45 2010 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Mon Feb 15 08:30:46 2010 Subject: [Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17 In-Reply-To: References: <208330218E165F45AB6307B787F5EA4761CD14115F@RAD-VMSRVEXV2.rad.wustl.edu>, Message-ID: One more thing.... This was my reply to Lynn and her response to my question. Fawn ________________________________________ From: Fawn Bomar Sent: Monday, February 15, 2010 9:24 AM To: Jones, Lynne Subject: RE: FYI - Histonet Digest, Vol 75, Issue 17 Hi Lynne, My inquiry about the Her2 validation was to get overall suggestions on how different labs interpreted how to go about the validation process. I said that we had a process but since I was new to the clinical aspect and to immuno's in general, I wanted to make sure that we were up to par with everyone else or if I needed to make adjustments to make our validation better. I was making sure that we met or exceeded expectations and I felt that everyone could offer an opinion. There should be no doubt in confidence in our accreditation...there should be some comfort in knowing that I am making sure that we are doing the best work that can be done. Sincerely Fawn ________________________________________ From: Jones, Lynne [jonesly@mir.wustl.edu] Sent: Friday, February 12, 2010 5:48 PM To: Fawn Bomar Subject: FYI - Histonet Digest, Vol 75, Issue 17 Hello - I was curious about the facility where you worked, so I looked up Halifax Regional, but it seems congratulations are in order, since Gwen Wade just posted a Press release indicating that the lab passed CAP accreditation. (Though I'm not sure your inquiry about a fundamental process in a histology lab would inspire confidence.) HistoNet is a fabulous resource, but there are some questions you may want to ask using a less traceable e-mail account. HistoNet archives are readily accessible, even to the general public. I do understand the panic and second-guessing that hits right before the inspectors appear, but you obviously had all the I's dotted and t's crossed. The next time will be easier. :) Take care, Lynne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet- ------------------------------ Message: 3 Date: Thu, 11 Feb 2010 14:06:56 -0500 From: "Fawn Bomar" Subject: [Histonet] Help... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 4 Date: Thu, 11 Feb 2010 12:28:27 -0700 From: "Liz Chlipala" Subject: RE: [Histonet] Help... To: "Fawn Bomar" , Message-ID: Content-Type: text/plain; charset="us-ascii" Fawn CAP has specific quidelines, its right there in the questions, did you do what that question is asking and have you documented what was done. They tell you right in the check sheet what needs to be completed. Test validation must be performed on a minimum of 25 cases (they recommend 25- 100) and they tell you how to validate too. There is also an article that you can get on the CAP website on IHC standardization. I have a pdf of it here if you need it. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Thursday, February 11, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help... Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 17 **************************************** The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From histonet.nospam <@t> vneubert.com Mon Feb 15 08:55:00 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Mon Feb 15 08:55:07 2010 Subject: [Histonet] IHC TBS wash buffer In-Reply-To: <24D22DE9E488AA43BF92A4389F2DDB1F0ECA0BC3@mail1.BOSTWICK.COM> References: <24D22DE9E488AA43BF92A4389F2DDB1F0ECA0BC3@mail1.BOSTWICK.COM> Message-ID: <4B796044.9080301@vneubert.com> 7,6 ;) > Can anyone tell me what an acceptable pH range for IHC TBS wash buffer > is? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JPeters <@t> bostwicklaboratories.com Mon Feb 15 09:45:35 2010 From: JPeters <@t> bostwicklaboratories.com (Justin Peters) Date: Mon Feb 15 09:45:41 2010 Subject: [Histonet] November 2009 copy of ASCP Lab Medicine journal Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F0ECA0E04@mail1.BOSTWICK.COM> Does anyone have a copy of the November 2009 ASCP Lab Medicine journal that they wouldn't mind parting with? I have a colleague who's wife had a paper published in that edition and he would like to have a copy. Thanks! Justin Peters, HTL (ASCP) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 From rjbuesa <@t> yahoo.com Mon Feb 15 09:50:18 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 15 09:50:21 2010 Subject: [Histonet] November 2009 copy of ASCP Lab Medicine journal In-Reply-To: <24D22DE9E488AA43BF92A4389F2DDB1F0ECA0E04@mail1.BOSTWICK.COM> Message-ID: <288692.27975.qm@web65708.mail.ac4.yahoo.com> Just out of curiosity: isn't your colleague's wife entitled to a copy of the issue where she published the article? Ren? J. --- On Mon, 2/15/10, Justin Peters wrote: From: Justin Peters Subject: [Histonet] November 2009 copy of ASCP Lab Medicine journal To: histonet@lists.utsouthwestern.edu Date: Monday, February 15, 2010, 10:45 AM Does anyone have a copy of the November 2009 ASCP Lab Medicine journal that they wouldn't mind parting with?? I have a colleague who's wife had a paper published in that edition and he would like to have a copy. Thanks! Justin Peters, HTL (ASCP) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone:? ? (804) 967-9225 ext. 1831 Cell:? ? ? ? (804) 822-6084 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john <@t> imebinc.com Mon Feb 15 12:07:05 2010 From: john <@t> imebinc.com (John O'Brien) Date: Mon Feb 15 12:01:32 2010 Subject: [Histonet] RE: Histonet Digest, Vol 75, Issue 18 Message-ID: <01b001caae69$afb40490$4a01a8c0@EXECUTIVE01> Tery, Have you address this question fro Histo Net.? Let me Know John -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Does anyone use Rainbow Frost slides from IMEB ? (Percival Karen) 2. FT Job in Houston (Near Bush Airport) (Stella Mireles) 3. RE: Does anyone use Rainbow Frost slides from IMEB ? (R J VAZQUEZ) 4. Re: Does anyone use Rainbow Frost slides from IMEB (Hobbs, Carl) 5. RE: Hypothetical question (Bonner, Janet) 6. QIHC (jfi786@aol.com) 7. Re: QIHC (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Fri, 12 Feb 2010 13:00:07 -0500 From: "Percival Karen" Subject: Re: [Histonet] Does anyone use Rainbow Frost slides from IMEB ? To: "HistoLab" , Message-ID: <4B7550D702000011001F89DF@gv01a67m.gv.us.pri.wyeth.com> Content-Type: text/plain; charset=US-ASCII On that note, we don't have a problem with clean slides, but the coverslips are dusty as can be. Any suggestions out there? Karen >>> HistoLab 2/12/2010 12:57 PM >>> Scott, I would be happy to send you samples of our colored end slides or charged slides. Please let me know if you are interested. Regards, Matt Gorilla Scientific 1-866-435-4977 www.GorillaScientific.com Hi, We have been looking for a better, cleaner slide to use in our lab. We tried some samples from IMEB out in California that were cleaner than the Erie slides we have been using forever. Does anyone use the Rainbow Frost slides ? Any suggestions on quality slides? Also what is the typical price per gross for good, clean, consistent slides? These are on sale for $7.20 per gross. Thanks for all of the suggestions in advance, Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Fri, 12 Feb 2010 12:03:43 -0600 From: Stella Mireles Subject: [Histonet] FT Job in Houston (Near Bush Airport) To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We have a FT job opening for Hermann Hospital Northeast. Routine Histo work. Heavy bx's. Weekends off. Please contact the Hermann Hospital Website. Need more info - 281 540 6275 Thanks Stella ------------------------------ Message: 3 Date: Fri, 12 Feb 2010 11:11:35 -0800 From: R J VAZQUEZ Subject: RE: [Histonet] Does anyone use Rainbow Frost slides from IMEB ? To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Scott, Rex Johnson of Creative Waste has a great deal on rainbow colored end slides, with a vast section to choose from. His phone number is 1(888)795-8300. He is based out of Portland Oregon, but I am sure he can send them to you. Robyn > Date: Fri, 12 Feb 2010 12:57:11 -0500 > From: histosearch@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Does anyone use Rainbow Frost slides from IMEB ? > > Scott, > > I would be happy to send you samples of our colored end slides or > charged slides. Please let me know if you are interested. > > Regards, > > Matt > Gorilla Scientific > 1-866-435-4977 > www.GorillaScientific.com > > Hi, > > We have been looking for a better, cleaner slide to use in our lab. We > tried some samples from IMEB out in California that were cleaner than > the Erie slides we have been using forever. Does anyone use the > Rainbow Frost slides ? Any suggestions on quality slides? Also what is > the typical price per gross for good, clean, consistent slides? These > are on sale for $7.20 per gross. > > Thanks for all of the suggestions in advance, > > Scott Hendricksen HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 12 Feb 2010 19:51:50 +0000 From: "Hobbs, Carl" Subject: [Histonet] Re: Does anyone use Rainbow Frost slides from IMEB To: "histonet@lists.utsouthwestern.edu" Message-ID: <11D9615B89C10747B1C985966A63D7CA2C5C88B1F6@KCL-MAIL04.kclad.ds.kcl.ac.u k> Content-Type: text/plain; charset="us-ascii" I may have missed your explanatory post so, my apologies. How do you know that your slides are "dirty" ? Thanks, carl ------------------------------ Message: 5 Date: Fri, 12 Feb 2010 16:00:21 -0500 From: "Bonner, Janet" Subject: RE: [Histonet] Hypothetical question To: "Linke, Noelle" , Kim.Donadio@bhcpns.org, histonet@lists.utsouthwestern.edu Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2CC5@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 Uh....Not today. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Linke, Noelle Sent: Fri 2/12/2010 12:46 PM To: Kim.Donadio@bhcpns.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Hypothetical question Hahahaha....cranky staff?? I'm not a big fan of Florida....too muggy, makes my hair crazy. No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services UCLA Department of Pathology and Laboratory Medicine Phone: 310-825-7397 Fax: 310-983-3289 nlinke@mednet.ucla.edu Pager: 97471 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Friday, February 12, 2010 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hypothetical question Out of curiosity, how many Histotechs would like to work in a lab where you have an average of 100-150 blocks per day, a hand full of special stains at most which are done on the Dako artisan, IHC done on the Ventana XT and less than a hand full of cytology preps. You might have 0-5 frozen sections per day as well . You would even have help from one or two other technologist. You could work 4/10 hour days or 5/8 hour days. Do any of you think this sounds like too much work? Maybe this hypothetical lab is located in the beautiful area of Pensacola Fl? All of this is Hypothetical... <<<< Again, I'm just curious :-) Thanks Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 6 Date: Fri, 12 Feb 2010 20:22:01 -0500 From: jfi786@aol.com Subject: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu Message-ID: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj ------------------------------ Message: 7 Date: Sat, 13 Feb 2010 07:21:28 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu, jfi786@aol.com Message-ID: <253066.94334.qm@web65702.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Contact the NSH because I am sure they will be able to help you. Ren? J. --- On Fri, 2/12/10, jfi786@aol.com wrote: From: jfi786@aol.com Subject: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu Date: Friday, February 12, 2010, 8:22 PM Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 18 **************************************** From nancy.troiano <@t> yale.edu Mon Feb 15 12:29:06 2010 From: nancy.troiano <@t> yale.edu (Nancy Troiano) Date: Mon Feb 15 12:29:16 2010 Subject: [Histonet] Region I 2010 Symposium In-Reply-To: <201002151800.o1FI0xDB023716@mr6.its.yale.edu> References: <201002151800.o1FI0xDB023716@mr6.its.yale.edu> Message-ID: <000001caae6c$c327d8f0$9fd48482@yu.yale.edu> The Region I symposium (New England and New York) will be held on April 23,24, 2010 in Mystic Connecticut. Check out the course listings/registration at www.connhisto.org. and join us! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, February 15, 2010 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Hi to all............. (Don Hammer) 2. IHC TBS wash buffer (Justin Peters) 3. Florida Society for Histotechnology-Region III Meeting (Jerry Santiago) 4. QIHC (Walter Benton) 5. FW: FYI - Histonet Digest, Vol 75, Issue 17 (Fawn Bomar) 6. FW: FYI - Histonet Digest, Vol 75, Issue 17 (Fawn Bomar) 7. Re: IHC TBS wash buffer (V. Neubert) 8. November 2009 copy of ASCP Lab Medicine journal (Justin Peters) 9. Re: November 2009 copy of ASCP Lab Medicine journal (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sun, 14 Feb 2010 22:02:27 -0800 From: "Don Hammer" Subject: [Histonet] Hi to all............. To: "histonet" Message-ID: <281414D805204848BA1CC3D07AB02D96@Don> Content-Type: text/plain; charset="iso-8859-1" Hi Histology Professionals, I just finished reading the recent issue of "NSH In Action", noting a reference to "Histonet" so thought to drop in to see how this very important, long lasting exchange of information is going. Congrats Linda with thanks to the UTSouthwestern for supporting the site. (14 years now I believe) Perhaps there are a few colleagues still here, continuing to offer assistance from their wealth of experience and knowledge, from back in my Laboratory days (or was that Daze?) :>) It's been near 12 years since retiring, wouldn't give it up for anything, but often think of you all who are in the Profession and now preparing for "Histotechnology Professionals Day" Such a great opportunity to blow your own horn a bit while making the general public aware of your support in the various facets of Medicine. Don't forget to celebrate all you do on a daily basis. I'm completely blown away at how NSH has grown from our Founding Meeting 36 years ago with it's extensive Symposium/Convention, Educational Materials, Journal of Histotechnology, Summer Symposium's, Teleconferences, Specialty Forums, Awards, Positions with or on Boards of National Organizations, just to highlight a few. When viewing the Website containing so much info for Members it's hard to believe much of it was kept on Card files and File drawers manned by one person in the Office. What's difficult to understand is the total Membership is nearly the same number. Well just sending some thoughts. Think I figured out how to post a comment, now I need to go figure out how to view the daily posts on "Histonet" It's been so long since doing that. Don Hammer, Retired Guy " I can't remember the last time I wasn't at least kind of tired." "I totally take back all those times I didn't want to nap when I was younger" ------------------------------ Message: 2 Date: Mon, 15 Feb 2010 08:24:41 -0500 From: "Justin Peters" Subject: [Histonet] IHC TBS wash buffer To: Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F0ECA0BC3@mail1.BOSTWICK.COM> Content-Type: text/plain; charset="us-ascii" Can anyone tell me what an acceptable pH range for IHC TBS wash buffer is? ------------------------------ Message: 3 Date: Mon, 15 Feb 2010 05:29:08 -0800 (PST) From: Jerry Santiago Subject: [Histonet] Florida Society for Histotechnology-Region III Meeting To: histonet@lists.utsouthwestern.edu Message-ID: <967631.97107.qm@web180415.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii Dear Histonetters, The program and online registration for the Florida Society for Histotechnology Meeting is now available at www.fshgroup.org. You can also go directly to the online registration process by visiting www.fshmeeting.com. Sincerely, Jerry Santiago, BS, HTL(ASCP)QIHC NSH Region III Director Shands Jacksonville T: 904-244-6149 E-mail: santij1@bellsouth.net ------------------------------ Message: 4 Date: Mon, 15 Feb 2010 09:13:14 -0500 From: "Walter Benton" Subject: [Histonet] QIHC To: Message-ID: <4B790FC5.D886.00F4.3@umm.edu> Content-Type: text/plain; charset=US-ASCII I recently took and passed the examination . I used the Dako book, material from Ethel Macrea's presentation, NSH study guide and material from the other references on the list. Ethel can be reached at emacrea@swskinpath.com She is a wonderful resource. Message: 6 Date: Fri, 12 Feb 2010 20:22:01 -0500 From: jfi786@aol.com Subject: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu Message-ID: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 5 Date: Mon, 15 Feb 2010 09:24:26 -0500 From: "Fawn Bomar" Subject: [Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii Hi everyone, Since I received this e-mail from Lynne about a question that I asked, I decided it was probably in my best interest not to ask any more questions in general. Thank you to everyone that helped verify that my validation process was indeed up to par! I will stay on the histonet in hopes that I can help others but I will keep my own questions to myself Fawn ________________________________________ From: Jones, Lynne [jonesly@mir.wustl.edu] Sent: Friday, February 12, 2010 5:48 PM To: Fawn Bomar Subject: FYI - Histonet Digest, Vol 75, Issue 17 Hello - I was curious about the facility where you worked, so I looked up Halifax Regional, but it seems congratulations are in order, since Gwen Wade just posted a Press release indicating that the lab passed CAP accreditation. (Though I'm not sure your inquiry about a fundamental process in a histology lab would inspire confidence.) HistoNet is a fabulous resource, but there are some questions you may want to ask using a less traceable e-mail account. HistoNet archives are readily accessible, even to the general public. I do understand the panic and second-guessing that hits right before the inspectors appear, but you obviously had all the I's dotted and t's crossed. The next time will be easier. :) Take care, Lynne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet- ------------------------------ Message: 3 Date: Thu, 11 Feb 2010 14:06:56 -0500 From: "Fawn Bomar" Subject: [Histonet] Help... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 4 Date: Thu, 11 Feb 2010 12:28:27 -0700 From: "Liz Chlipala" Subject: RE: [Histonet] Help... To: "Fawn Bomar" , Message-ID: Content-Type: text/plain; charset="us-ascii" Fawn CAP has specific quidelines, its right there in the questions, did you do what that question is asking and have you documented what was done. They tell you right in the check sheet what needs to be completed. Test validation must be performed on a minimum of 25 cases (they recommend 25- 100) and they tell you how to validate too. There is also an article that you can get on the CAP website on IHC standardization. I have a pdf of it here if you need it. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Thursday, February 11, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help... Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 17 **************************************** The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. ------------------------------ Message: 6 Date: Mon, 15 Feb 2010 09:29:45 -0500 From: "Fawn Bomar" Subject: [Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii One more thing.... This was my reply to Lynn and her response to my question. Fawn ________________________________________ From: Fawn Bomar Sent: Monday, February 15, 2010 9:24 AM To: Jones, Lynne Subject: RE: FYI - Histonet Digest, Vol 75, Issue 17 Hi Lynne, My inquiry about the Her2 validation was to get overall suggestions on how different labs interpreted how to go about the validation process. I said that we had a process but since I was new to the clinical aspect and to immuno's in general, I wanted to make sure that we were up to par with everyone else or if I needed to make adjustments to make our validation better. I was making sure that we met or exceeded expectations and I felt that everyone could offer an opinion. There should be no doubt in confidence in our accreditation...there should be some comfort in knowing that I am making sure that we are doing the best work that can be done. Sincerely Fawn ________________________________________ From: Jones, Lynne [jonesly@mir.wustl.edu] Sent: Friday, February 12, 2010 5:48 PM To: Fawn Bomar Subject: FYI - Histonet Digest, Vol 75, Issue 17 Hello - I was curious about the facility where you worked, so I looked up Halifax Regional, but it seems congratulations are in order, since Gwen Wade just posted a Press release indicating that the lab passed CAP accreditation. (Though I'm not sure your inquiry about a fundamental process in a histology lab would inspire confidence.) HistoNet is a fabulous resource, but there are some questions you may want to ask using a less traceable e-mail account. HistoNet archives are readily accessible, even to the general public. I do understand the panic and second-guessing that hits right before the inspectors appear, but you obviously had all the I's dotted and t's crossed. The next time will be easier. :) Take care, Lynne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet- ------------------------------ Message: 3 Date: Thu, 11 Feb 2010 14:06:56 -0500 From: "Fawn Bomar" Subject: [Histonet] Help... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 4 Date: Thu, 11 Feb 2010 12:28:27 -0700 From: "Liz Chlipala" Subject: RE: [Histonet] Help... To: "Fawn Bomar" , Message-ID: Content-Type: text/plain; charset="us-ascii" Fawn CAP has specific quidelines, its right there in the questions, did you do what that question is asking and have you documented what was done. They tell you right in the check sheet what needs to be completed. Test validation must be performed on a minimum of 25 cases (they recommend 25- 100) and they tell you how to validate too. There is also an article that you can get on the CAP website on IHC standardization. I have a pdf of it here if you need it. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Thursday, February 11, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help... Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 17 **************************************** The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. ------------------------------ Message: 7 Date: Mon, 15 Feb 2010 15:55:00 +0100 From: "V. Neubert" Subject: Re: [Histonet] IHC TBS wash buffer To: histonet@lists.utsouthwestern.edu Message-ID: <4B796044.9080301@vneubert.com> Content-Type: text/plain; charset=ISO-8859-1 7,6 ;) > Can anyone tell me what an acceptable pH range for IHC TBS wash buffer > is? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Mon, 15 Feb 2010 10:45:35 -0500 From: "Justin Peters" Subject: [Histonet] November 2009 copy of ASCP Lab Medicine journal To: Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F0ECA0E04@mail1.BOSTWICK.COM> Content-Type: text/plain; charset="us-ascii" Does anyone have a copy of the November 2009 ASCP Lab Medicine journal that they wouldn't mind parting with? I have a colleague who's wife had a paper published in that edition and he would like to have a copy. Thanks! Justin Peters, HTL (ASCP) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 ------------------------------ Message: 9 Date: Mon, 15 Feb 2010 07:50:18 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] November 2009 copy of ASCP Lab Medicine journal To: histonet@lists.utsouthwestern.edu, Justin Peters Message-ID: <288692.27975.qm@web65708.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Just out of curiosity: isn't your colleague's wife entitled to a copy of the issue where she published the article? Reni J. --- On Mon, 2/15/10, Justin Peters wrote: From: Justin Peters Subject: [Histonet] November 2009 copy of ASCP Lab Medicine journal To: histonet@lists.utsouthwestern.edu Date: Monday, February 15, 2010, 10:45 AM Does anyone have a copy of the November 2009 ASCP Lab Medicine journal that they wouldn't mind parting with? I have a colleague who's wife had a paper published in that edition and he would like to have a copy. Thanks! Justin Peters, HTL (ASCP) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 20 **************************************** From beth.millerman <@t> stiefel.com Mon Feb 15 13:19:23 2010 From: beth.millerman <@t> stiefel.com (Beth Millerman) Date: Mon Feb 15 13:19:35 2010 Subject: [Histonet] Ultralight Message-ID: Felton, My lab is Dermatology research and processes skin samples only. I have tried the UltraLight Fixative by Pacific Southwest and found that it works quite nicely . My H&E are picture perfect, and my receptors on my IHC's stain well. I use it because it doesn't seem to be as harsh or drying as 10% Formalin. Beth Millerman/SWC Scientist Stiefel Laboratories, Inc,, a GSK company Direct Dial; 650-739-2982 From flnails <@t> texaschildrens.org Mon Feb 15 13:40:46 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon Feb 15 13:41:01 2010 Subject: [Histonet] Ultralight In-Reply-To: References: Message-ID: Thanks Beth, I have not had a lot feedback on this question. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Millerman Sent: Monday, February 15, 2010 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ultralight Felton, My lab is Dermatology research and processes skin samples only. I have tried the UltraLight Fixative by Pacific Southwest and found that it works quite nicely . My H&E are picture perfect, and my receptors on my IHC's stain well. I use it because it doesn't seem to be as harsh or drying as 10% Formalin. Beth Millerman/SWC Scientist Stiefel Laboratories, Inc,, a GSK company Direct Dial; 650-739-2982 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From erweber <@t> maxhealth.com Mon Feb 15 13:55:12 2010 From: erweber <@t> maxhealth.com (Eric Weber) Date: Mon Feb 15 13:55:24 2010 Subject: [Histonet] Contact Histo Tech Needed w/ VAMC Experience Message-ID: <9D12D4EF30176D4F839EB47F3D0E843612AB7873@exbk2.maxhealth.com> My name is Eric Weber and I am a recruiter for Maxim Government Services. We are one of the leading providers of contract medical personnel to the Veteran Affairs Medical Centers. I'm currently recruiting for a Histo Tech for a long term (6 months) contract in Southern California. This contract is full time and at a Veteran Affairs Medical Center. Our client has requested candidates with previous VAMC experience. "Vetpro" credentialed is strongly preferred. Please contact me if you have previous VAMC experience and would be interested in a contract assignment in Southern California. Travel candidates will be considered. Thank You, Eric Weber Maxim Government Services 7227 Lee DeForest Dr Columbia, MD 21046 phone: (410) 910-4942 toll free: (866) 260-9142 fax:(410) 953-8358 erweber@maxhealth.com CONFIDENTIALITY NOTE: This e-mail message contains confidential, privileged or otherwise protected information intended solely for the addressee. Please do not read, copy, or disseminate it unless you are the intended addressee. If you have received it in error, please call us (collect) at (410) 910-1500 and ask to speak with the message sender. Also, we would appreciate your forwarding the message back to us and deleting it from your system. Thank you. This e-mail and all other electronic (including voice) communications from the sender's firm are for informational purposes only. No such communication is intended by the sender to constitute either an electronic record or an electronic signature or to constitute any agreement by the sender to conduct a transaction by electronic means. Any such intention or agreement is hereby expressly disclaimed unless otherwise specifically indicated. From Laura.Miller <@t> leica-microsystems.com Mon Feb 15 16:02:13 2010 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Mon Feb 15 16:02:23 2010 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 02/15/2010 and will not return until 02/16/2010. I am on vacation today. I will respond to you tomorrow when I return. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From j_amiri <@t> modares.ac.ir Tue Feb 16 07:03:34 2010 From: j_amiri <@t> modares.ac.ir (Jamshid Amiri Moghaddam) Date: Tue Feb 16 07:01:52 2010 Subject: [Histonet] SEM for Spermatozoa Message-ID: <001601caaf08$726596e0$5730c4a0$@ac.ir> Hi every body We want to scan fish spermatozoa By SEM. But I don't know the method for dehydration after Post fixation with osmium tetra oxide? By ethanol series or aceton? I thanks to all who can help me in SEM for Spermatozoa. Regards: Jamshid -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Hi to all............. (Don Hammer) 2. IHC TBS wash buffer (Justin Peters) 3. Florida Society for Histotechnology-Region III Meeting (Jerry Santiago) 4. QIHC (Walter Benton) 5. FW: FYI - Histonet Digest, Vol 75, Issue 17 (Fawn Bomar) 6. FW: FYI - Histonet Digest, Vol 75, Issue 17 (Fawn Bomar) 7. Re: IHC TBS wash buffer (V. Neubert) 8. November 2009 copy of ASCP Lab Medicine journal (Justin Peters) 9. Re: November 2009 copy of ASCP Lab Medicine journal (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sun, 14 Feb 2010 22:02:27 -0800 From: "Don Hammer" Subject: [Histonet] Hi to all............. To: "histonet" Message-ID: <281414D805204848BA1CC3D07AB02D96@Don> Content-Type: text/plain; charset="iso-8859-1" Hi Histology Professionals, I just finished reading the recent issue of "NSH In Action", noting a reference to "Histonet" so thought to drop in to see how this very important, long lasting exchange of information is going. Congrats Linda with thanks to the UTSouthwestern for supporting the site. (14 years now I believe) Perhaps there are a few colleagues still here, continuing to offer assistance from their wealth of experience and knowledge, from back in my Laboratory days (or was that Daze?) :>) It's been near 12 years since retiring, wouldn't give it up for anything, but often think of you all who are in the Profession and now preparing for "Histotechnology Professionals Day" Such a great opportunity to blow your own horn a bit while making the general public aware of your support in the various facets of Medicine. Don't forget to celebrate all you do on a daily basis. I'm completely blown away at how NSH has grown from our Founding Meeting 36 years ago with it's extensive Symposium/Convention, Educational Materials, Journal of Histotechnology, Summer Symposium's, Teleconferences, Specialty Forums, Awards, Positions with or on Boards of National Organizations, just to highlight a few. When viewing the Website containing so much info for Members it's hard to believe much of it was kept on Card files and File drawers manned by one person in the Office. What's difficult to understand is the total Membership is nearly the same number. Well just sending some thoughts. Think I figured out how to post a comment, now I need to go figure out how to view the daily posts on "Histonet" It's been so long since doing that. Don Hammer, Retired Guy " I can't remember the last time I wasn't at least kind of tired." "I totally take back all those times I didn't want to nap when I was younger" ------------------------------ Message: 2 Date: Mon, 15 Feb 2010 08:24:41 -0500 From: "Justin Peters" Subject: [Histonet] IHC TBS wash buffer To: Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F0ECA0BC3@mail1.BOSTWICK.COM> Content-Type: text/plain; charset="us-ascii" Can anyone tell me what an acceptable pH range for IHC TBS wash buffer is? ------------------------------ Message: 3 Date: Mon, 15 Feb 2010 05:29:08 -0800 (PST) From: Jerry Santiago Subject: [Histonet] Florida Society for Histotechnology-Region III Meeting To: histonet@lists.utsouthwestern.edu Message-ID: <967631.97107.qm@web180415.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii Dear Histonetters, The program and online registration for the Florida Society for Histotechnology Meeting is now available at www.fshgroup.org. You can also go directly to the online registration process by visiting www.fshmeeting.com. Sincerely, Jerry Santiago, BS, HTL(ASCP)QIHC NSH Region III Director Shands Jacksonville T: 904-244-6149 E-mail: santij1@bellsouth.net ------------------------------ Message: 4 Date: Mon, 15 Feb 2010 09:13:14 -0500 From: "Walter Benton" Subject: [Histonet] QIHC To: Message-ID: <4B790FC5.D886.00F4.3@umm.edu> Content-Type: text/plain; charset=US-ASCII I recently took and passed the examination . I used the Dako book, material from Ethel Macrea's presentation, NSH study guide and material from the other references on the list. Ethel can be reached at emacrea@swskinpath.com She is a wonderful resource. Message: 6 Date: Fri, 12 Feb 2010 20:22:01 -0500 From: jfi786@aol.com Subject: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu Message-ID: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 5 Date: Mon, 15 Feb 2010 09:24:26 -0500 From: "Fawn Bomar" Subject: [Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii Hi everyone, Since I received this e-mail from Lynne about a question that I asked, I decided it was probably in my best interest not to ask any more questions in general. Thank you to everyone that helped verify that my validation process was indeed up to par! I will stay on the histonet in hopes that I can help others but I will keep my own questions to myself Fawn ________________________________________ From: Jones, Lynne [jonesly@mir.wustl.edu] Sent: Friday, February 12, 2010 5:48 PM To: Fawn Bomar Subject: FYI - Histonet Digest, Vol 75, Issue 17 Hello - I was curious about the facility where you worked, so I looked up Halifax Regional, but it seems congratulations are in order, since Gwen Wade just posted a Press release indicating that the lab passed CAP accreditation. (Though I'm not sure your inquiry about a fundamental process in a histology lab would inspire confidence.) HistoNet is a fabulous resource, but there are some questions you may want to ask using a less traceable e-mail account. HistoNet archives are readily accessible, even to the general public. I do understand the panic and second-guessing that hits right before the inspectors appear, but you obviously had all the I's dotted and t's crossed. The next time will be easier. :) Take care, Lynne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet- ------------------------------ Message: 3 Date: Thu, 11 Feb 2010 14:06:56 -0500 From: "Fawn Bomar" Subject: [Histonet] Help... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 4 Date: Thu, 11 Feb 2010 12:28:27 -0700 From: "Liz Chlipala" Subject: RE: [Histonet] Help... To: "Fawn Bomar" , Message-ID: Content-Type: text/plain; charset="us-ascii" Fawn CAP has specific quidelines, its right there in the questions, did you do what that question is asking and have you documented what was done. They tell you right in the check sheet what needs to be completed. Test validation must be performed on a minimum of 25 cases (they recommend 25- 100) and they tell you how to validate too. There is also an article that you can get on the CAP website on IHC standardization. I have a pdf of it here if you need it. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Thursday, February 11, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help... Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 17 **************************************** The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. ------------------------------ Message: 6 Date: Mon, 15 Feb 2010 09:29:45 -0500 From: "Fawn Bomar" Subject: [Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii One more thing.... This was my reply to Lynn and her response to my question. Fawn ________________________________________ From: Fawn Bomar Sent: Monday, February 15, 2010 9:24 AM To: Jones, Lynne Subject: RE: FYI - Histonet Digest, Vol 75, Issue 17 Hi Lynne, My inquiry about the Her2 validation was to get overall suggestions on how different labs interpreted how to go about the validation process. I said that we had a process but since I was new to the clinical aspect and to immuno's in general, I wanted to make sure that we were up to par with everyone else or if I needed to make adjustments to make our validation better. I was making sure that we met or exceeded expectations and I felt that everyone could offer an opinion. There should be no doubt in confidence in our accreditation...there should be some comfort in knowing that I am making sure that we are doing the best work that can be done. Sincerely Fawn ________________________________________ From: Jones, Lynne [jonesly@mir.wustl.edu] Sent: Friday, February 12, 2010 5:48 PM To: Fawn Bomar Subject: FYI - Histonet Digest, Vol 75, Issue 17 Hello - I was curious about the facility where you worked, so I looked up Halifax Regional, but it seems congratulations are in order, since Gwen Wade just posted a Press release indicating that the lab passed CAP accreditation. (Though I'm not sure your inquiry about a fundamental process in a histology lab would inspire confidence.) HistoNet is a fabulous resource, but there are some questions you may want to ask using a less traceable e-mail account. HistoNet archives are readily accessible, even to the general public. I do understand the panic and second-guessing that hits right before the inspectors appear, but you obviously had all the I's dotted and t's crossed. The next time will be easier. :) Take care, Lynne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet- ------------------------------ Message: 3 Date: Thu, 11 Feb 2010 14:06:56 -0500 From: "Fawn Bomar" Subject: [Histonet] Help... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 4 Date: Thu, 11 Feb 2010 12:28:27 -0700 From: "Liz Chlipala" Subject: RE: [Histonet] Help... To: "Fawn Bomar" , Message-ID: Content-Type: text/plain; charset="us-ascii" Fawn CAP has specific quidelines, its right there in the questions, did you do what that question is asking and have you documented what was done. They tell you right in the check sheet what needs to be completed. Test validation must be performed on a minimum of 25 cases (they recommend 25- 100) and they tell you how to validate too. There is also an article that you can get on the CAP website on IHC standardization. I have a pdf of it here if you need it. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Thursday, February 11, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help... Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 17 **************************************** The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. ------------------------------ Message: 7 Date: Mon, 15 Feb 2010 15:55:00 +0100 From: "V. Neubert" Subject: Re: [Histonet] IHC TBS wash buffer To: histonet@lists.utsouthwestern.edu Message-ID: <4B796044.9080301@vneubert.com> Content-Type: text/plain; charset=ISO-8859-1 7,6 ;) > Can anyone tell me what an acceptable pH range for IHC TBS wash buffer > is? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Mon, 15 Feb 2010 10:45:35 -0500 From: "Justin Peters" Subject: [Histonet] November 2009 copy of ASCP Lab Medicine journal To: Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F0ECA0E04@mail1.BOSTWICK.COM> Content-Type: text/plain; charset="us-ascii" Does anyone have a copy of the November 2009 ASCP Lab Medicine journal that they wouldn't mind parting with? I have a colleague who's wife had a paper published in that edition and he would like to have a copy. Thanks! Justin Peters, HTL (ASCP) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 ------------------------------ Message: 9 Date: Mon, 15 Feb 2010 07:50:18 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] November 2009 copy of ASCP Lab Medicine journal To: histonet@lists.utsouthwestern.edu, Justin Peters Message-ID: <288692.27975.qm@web65708.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Just out of curiosity: isn't your colleague's wife entitled to a copy of the issue where she published the article? Reni J. --- On Mon, 2/15/10, Justin Peters wrote: From: Justin Peters Subject: [Histonet] November 2009 copy of ASCP Lab Medicine journal To: histonet@lists.utsouthwestern.edu Date: Monday, February 15, 2010, 10:45 AM Does anyone have a copy of the November 2009 ASCP Lab Medicine journal that they wouldn't mind parting with? I have a colleague who's wife had a paper published in that edition and he would like to have a copy. Thanks! Justin Peters, HTL (ASCP) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 20 **************************************** __________ Information from ESET NOD32 Antivirus, version of virus signature database 4869 (20100215) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __________ Information from ESET NOD32 Antivirus, version of virus signature database 4869 (20100215) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com From histonetalias <@t> gmail.com Tue Feb 16 09:32:26 2010 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Tue Feb 16 09:32:31 2010 Subject: [Histonet] Hypothetical question In-Reply-To: References: Message-ID: <4b6c85511002160732h62c0af61yb4d1d6d49eb46509@mail.gmail.com> Ha Ha Kim! Are you recruiting? I think if you are used to cutting 50 blocks then 100 will be "too much". Most tech are used to their certain workload and if it increases or if they go to a bigger lab it will take some adjustment. On Fri, Feb 12, 2010 at 12:32 PM, wrote: > Out of curiosity, how many Histotechs would like to work in a lab where > you have an average of 100-150 blocks per day, a hand full of special > stains at most which are done on the Dako artisan, IHC done on the Ventana > XT and less than a hand full of cytology preps. You might have 0-5 frozen > sections per day as well > . > You would even have help from one or two other technologist. You could > work 4/10 hour days or 5/8 hour days. > > Do any of you think this sounds like too much work? > > Maybe this hypothetical lab is located in the beautiful area of Pensacola > Fl? > > All of this is Hypothetical... <<<< > > Again, I'm just curious :-) > > Thanks > > > > Kim Donadio > Pathology Supervisor > Baptist Hospital > 1000 W Moreno St. > Pensacola FL 32501 > Phone (850) 469-7718 > Fax (850) 434-4996 > > ----------------------------------------- > All electronic data transmissions originating from or sent to > Baptist Health Care Corporation (BHC) are subject to monitoring. > This message along with any attached data, are the confidential and > proprietary communications of BHC and are intended to be received > only by the individual or individuals to whom the message has been > addressed. If the reader of this message is not the intended > recipient, please take notice that any use, copying, printing, > forwarding or distribution of this message, in any form, is > strictly prohibited and may violate State or Federal Law. If you > have received this transmission in error, please delete or destroy > all copies of this message. For questions, contact the BHC Privacy > Officer at (850) 434-4472. Rev.10/07. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From histonetalias <@t> gmail.com Tue Feb 16 09:42:56 2010 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Tue Feb 16 09:43:01 2010 Subject: [Histonet] Michelle Rederick where did you go? Message-ID: <4b6c85511002160742x435997aaib1a120a73f122528@mail.gmail.com> *Does anyone have Michelle Rederick's contact info? She was working for Milestone.* -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From Kim.Donadio <@t> bhcpns.org Tue Feb 16 10:40:26 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Feb 16 10:40:50 2010 Subject: [Histonet] Hypothetical question In-Reply-To: <4b6c85511002160732h62c0af61yb4d1d6d49eb46509@mail.gmail.com> Message-ID: Me? Recruit, Never. I'm just inherently curious. I have to say though I'm glad I'm not, didn't get to many replies but I did find out why my hair is always a mess < wink> Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Histonet Alias 02/16/2010 09:32 AM To Kim.Donadio@bhcpns.org cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Hypothetical question Ha Ha Kim! Are you recruiting? I think if you are used to cutting 50 blocks then 100 will be "too much". Most tech are used to their certain workload and if it increases or if they go to a bigger lab it will take some adjustment. On Fri, Feb 12, 2010 at 12:32 PM, wrote: Out of curiosity, how many Histotechs would like to work in a lab where you have an average of 100-150 blocks per day, a hand full of special stains at most which are done on the Dako artisan, IHC done on the Ventana XT and less than a hand full of cytology preps. You might have 0-5 frozen sections per day as well . You would even have help from one or two other technologist. You could work 4/10 hour days or 5/8 hour days. Do any of you think this sounds like too much work? Maybe this hypothetical lab is located in the beautiful area of Pensacola Fl? All of this is Hypothetical... <<<< Again, I'm just curious :-) Thanks Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From ktuttle <@t> umm.edu Tue Feb 16 11:16:04 2010 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Tue Feb 16 11:16:14 2010 Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems Message-ID: <4B7A8C70.90CE.001A.3@umm.edu> Does anyone know who to contact to get this instrument serviced? It seems the company is no longer in business. The phone numbers I have dont work, and the website is no longer there. I found a company online who repairs them, but they are in Massachusetts, I am in Maryland so that would be my last resort. Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From katherine-walters <@t> uiowa.edu Tue Feb 16 11:33:54 2010 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Tue Feb 16 11:34:56 2010 Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems In-Reply-To: <4B7A8C70.90CE.001A.3@umm.edu> References: <4B7A8C70.90CE.001A.3@umm.edu> Message-ID: Molecular Devices bought them. Here is their website: http://www.moleculardevices.com/pages/instruments/microgenomics.html Hope this helps, Kathy Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walters@uiowa.edu www.uiowa.edu/~cemrf -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Tuesday, February 16, 2010 11:16 AM To: histonet Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems Does anyone know who to contact to get this instrument serviced? It seems the company is no longer in business. The phone numbers I have dont work, and the website is no longer there. I found a company online who repairs them, but they are in Massachusetts, I am in Maryland so that would be my last resort. Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Tue Feb 16 11:37:24 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Feb 16 11:39:57 2010 Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems In-Reply-To: References: <4B7A8C70.90CE.001A.3@umm.edu> Message-ID: <4B7AD7D4.1030400@pathology.washington.edu> And they have since been bought by.... *Danaher* Completes Acquisition of *AB SCIEX* and *Molecular Devices Corporation* (MDCC ) 2/1/2010 WASHINGTON, Feb. 1 /PRNewswire-FirstCall/ -- Danaher Corporation announced today that it has completed the previously announced acquisition of AB SCIEX and Molecular Devices. AB SCIEX is a leading designer and manufacturer of mass spectrometers, highly sensitive and sophisticated instruments used by researchers and clinicians to identify and quantify specific molecules in complex samples. Molecular Devices supplies high-performance bio-analytical instrumentation systems and consumables that accelerate and improve research productivity and effectiveness in life science research and drug discovery. Danaher Corporation Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Walters, Katherine S wrote: > Molecular Devices bought them. Here is their website: > http://www.moleculardevices.com/pages/instruments/microgenomics.html > > Hope this helps, > Kathy > > > > Katherine Walters > Histology Director > Central Microscopy Research Facilities > 85 Eckstein Medical Research Building > University of Iowa > Iowa City, Iowa 52242-1101 > > phone: # 319-335-8142 > fax: # 319-384-4469 > > katherine-walters@uiowa.edu > www.uiowa.edu/~cemrf > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly > Tuttle > Sent: Tuesday, February 16, 2010 11:16 AM > To: histonet > Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems > > Does anyone know who to contact to get this instrument serviced? It > seems the company is no longer in business. The phone numbers I have > dont work, and the website is no longer there. I found a company online > who repairs them, but they are in Massachusetts, I am in Maryland so > that would be my last resort. Thanks > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > Please consider the environment before printing this e-mail. > > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal law > protect the confidentiality of this information. If the reader of this > message is not the intended recipient; you are prohibited from using, > disclosing, reproducing or distributing this information; you should > immediately notify the sender by telephone or e-mail and delete this > e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From katherine-walters <@t> uiowa.edu Tue Feb 16 11:45:01 2010 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Tue Feb 16 11:45:58 2010 Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems In-Reply-To: <4B7AD7D4.1030400@pathology.washington.edu> References: <4B7A8C70.90CE.001A.3@umm.edu> <4B7AD7D4.1030400@pathology.washington.edu> Message-ID: GEEZ hard to keep track! Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walters@uiowa.edu www.uiowa.edu/~cemrf -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Tuesday, February 16, 2010 11:37 AM To: Walters, Katherine S Cc: Kimberly Tuttle; histonet Subject: Re: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems And they have since been bought by.... *Danaher* Completes Acquisition of *AB SCIEX* and *Molecular Devices Corporation* (MDCC ) 2/1/2010 WASHINGTON, Feb. 1 /PRNewswire-FirstCall/ -- Danaher Corporation announced today that it has completed the previously announced acquisition of AB SCIEX and Molecular Devices. AB SCIEX is a leading designer and manufacturer of mass spectrometers, highly sensitive and sophisticated instruments used by researchers and clinicians to identify and quantify specific molecules in complex samples. Molecular Devices supplies high-performance bio-analytical instrumentation systems and consumables that accelerate and improve research productivity and effectiveness in life science research and drug discovery. Danaher Corporation Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Walters, Katherine S wrote: > Molecular Devices bought them. Here is their website: > http://www.moleculardevices.com/pages/instruments/microgenomics.html > > Hope this helps, > Kathy > > > > Katherine Walters > Histology Director > Central Microscopy Research Facilities > 85 Eckstein Medical Research Building > University of Iowa > Iowa City, Iowa 52242-1101 > > phone: # 319-335-8142 > fax: # 319-384-4469 > > katherine-walters@uiowa.edu > www.uiowa.edu/~cemrf > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly > Tuttle > Sent: Tuesday, February 16, 2010 11:16 AM > To: histonet > Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems > > Does anyone know who to contact to get this instrument serviced? It > seems the company is no longer in business. The phone numbers I have > dont work, and the website is no longer there. I found a company online > who repairs them, but they are in Massachusetts, I am in Maryland so > that would be my last resort. Thanks > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > Please consider the environment before printing this e-mail. > > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal law > protect the confidentiality of this information. If the reader of this > message is not the intended recipient; you are prohibited from using, > disclosing, reproducing or distributing this information; you should > immediately notify the sender by telephone or e-mail and delete this > e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From KPercival <@t> wyeth.com Tue Feb 16 11:57:11 2010 From: KPercival <@t> wyeth.com (Percival Karen) Date: Tue Feb 16 11:57:24 2010 Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems In-Reply-To: <4B7A8C70.90CE.001A.3@umm.edu> References: <4B7A8C70.90CE.001A.3@umm.edu> Message-ID: <4B7A962702000011001F8B66@gv01a67m.gv.us.pri.wyeth.com> KImberly, I have an Arcturus Veritas LCM instrument. Contact Molecular Devices at 1 -800-635-5577. I believe they took over Arcturus business and supply matters, although Arcturus still manufactures the instrument. You can also access them online at www.moleculardevices.com Karen >>> "Kimberly Tuttle" 2/16/2010 12:16 PM >>> Does anyone know who to contact to get this instrument serviced? It seems the company is no longer in business. The phone numbers I have dont work, and the website is no longer there. I found a company online who repairs them, but they are in Massachusetts, I am in Maryland so that would be my last resort. Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Tue Feb 16 12:18:16 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Feb 16 12:18:21 2010 Subject: [Histonet] GSH membership/meeting Message-ID: <9BF995BC0E47744E9673A41486E24EE2242A273BFD@MERCERMAIL.MercerU.local> Hi Netters, Georgia histotechs and anyone else interested in coming to Georgia for a great meeting. I am reminding all about the GSH meeting March 26-27th at the Evergreen Marriott Convention Resort, Stone Mountain, Georgia. The deadline for receiving the member rates for the meeting was yesterday, Feb 15th, but we are extending the time to Feb 25th for those Georgians and neighboring states who have let time slip by. Join by the 15th and get the discounted rates for our meeting. To join online and for complete information, program, registration form and the hotel link directly to the Marriott reservations site go to our web site at www.histosearch.com/gsh and click on membership and then go to the symposium page to print out the forms. The deadline for registering for the meeting without a late fee is March 15th, but you need to be a member by the 25th of Feb. See you there. Shirley A. Powell, HT(ASCP)HTL, QIHC GSH Secretary Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From POWELL_SA <@t> mercer.edu Tue Feb 16 12:22:57 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Feb 16 12:23:01 2010 Subject: [Histonet] RE: GSH membership/meeting In-Reply-To: <9BF995BC0E47744E9673A41486E24EE2242A273BFD@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE2242A273BFD@MERCERMAIL.MercerU.local> Message-ID: <9BF995BC0E47744E9673A41486E24EE2242A273C14@MERCERMAIL.MercerU.local> Correction of dates, the meeting begins on Friday the 26th and goes through the 28th, not the 27th. Sorry. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, February 16, 2010 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GSH membership/meeting Hi Netters, Georgia histotechs and anyone else interested in coming to Georgia for a great meeting. I am reminding all about the GSH meeting March 26-27th at the Evergreen Marriott Convention Resort, Stone Mountain, Georgia. The deadline for receiving the member rates for the meeting was yesterday, Feb 15th, but we are extending the time to Feb 25th for those Georgians and neighboring states who have let time slip by. Join by the 15th and get the discounted rates for our meeting. To join online and for complete information, program, registration form and the hotel link directly to the Marriott reservations site go to our web site at www.histosearch.com/gsh and click on membership and then go to the symposium page to print out the forms. The deadline for registering for the meeting without a late fee is March 15th, but you need to be a member by the 25th of Feb. See you there. Shirley A. Powell, HT(ASCP)HTL, QIHC GSH Secretary Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sareenprashant <@t> hotmail.com Tue Feb 16 13:52:20 2010 From: sareenprashant <@t> hotmail.com (prashant sareen) Date: Tue Feb 16 13:52:26 2010 Subject: [Histonet] RE: Histonet Digest, Vol 75, Issue 22 In-Reply-To: References: Message-ID: Hey Kimberly, The company is Dolbey Jamison which does the repair and service for our company. Thanks Prashant Bioreliance 301-610-2297 > From: histonet-request@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 75, Issue 22 > To: histonet@lists.utsouthwestern.edu > Date: Tue, 16 Feb 2010 10:00:12 -0800 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Arcturus Laser Capture Microdissection (LCM) Systems > (Victor Tobias) > 2. RE: Arcturus Laser Capture Microdissection (LCM) Systems > (Walters, Katherine S) > 3. Re: Arcturus Laser Capture Microdissection (LCM) Systems > (Percival Karen) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 16 Feb 2010 09:37:24 -0800 > From: Victor Tobias > Subject: Re: [Histonet] Arcturus Laser Capture Microdissection (LCM) > Systems > To: "Walters, Katherine S" > Cc: histonet > Message-ID: <4B7AD7D4.1030400@pathology.washington.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > And they have since been bought by.... > > *Danaher* Completes Acquisition > of *AB SCIEX* and *Molecular > Devices Corporation* (MDCC > ) > 2/1/2010 > > WASHINGTON, Feb. 1 /PRNewswire-FirstCall/ -- Danaher Corporation > announced today that it has completed the previously announced > acquisition of AB SCIEX and Molecular Devices. AB SCIEX is a leading > designer and manufacturer of mass spectrometers, highly sensitive and > sophisticated instruments used by researchers and clinicians to identify > and quantify specific molecules in complex samples. Molecular Devices > supplies high-performance bio-analytical instrumentation systems and > consumables that accelerate and improve research productivity and > effectiveness in life science research and drug discovery. > > Danaher Corporation > > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > Walters, Katherine S wrote: > > Molecular Devices bought them. Here is their website: > > http://www.moleculardevices.com/pages/instruments/microgenomics.html > > > > Hope this helps, > > Kathy > > > > > > > > Katherine Walters > > Histology Director > > Central Microscopy Research Facilities > > 85 Eckstein Medical Research Building > > University of Iowa > > Iowa City, Iowa 52242-1101 > > > > phone: # 319-335-8142 > > fax: # 319-384-4469 > > > > katherine-walters@uiowa.edu > > www.uiowa.edu/~cemrf > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly > > Tuttle > > Sent: Tuesday, February 16, 2010 11:16 AM > > To: histonet > > Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems > > > > Does anyone know who to contact to get this instrument serviced? It > > seems the company is no longer in business. The phone numbers I have > > dont work, and the website is no longer there. I found a company online > > who repairs them, but they are in Massachusetts, I am in Maryland so > > that would be my last resort. Thanks > > > > Kimberly C. Tuttle HT (ASCP) > > Pathology Biorepository and Research Core > > University of Maryland > > Room NBW58, UMMC > > 22 S. Greene St > > Baltimore, MD 21201 > > (410) 328-5524 > > (410) 328-5508 fax > > Please consider the environment before printing this e-mail. > > > > > > > > > > This e-mail and any accompanying attachments may be privileged, > > confidential, contain protected health information about an identified > > patient or be otherwise protected from disclosure. State and federal law > > protect the confidentiality of this information. If the reader of this > > message is not the intended recipient; you are prohibited from using, > > disclosing, reproducing or distributing this information; you should > > immediately notify the sender by telephone or e-mail and delete this > > e-mail. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 16 Feb 2010 11:45:01 -0600 > From: "Walters, Katherine S" > Subject: RE: [Histonet] Arcturus Laser Capture Microdissection (LCM) > Systems > To: "Victor Tobias" > Cc: histonet > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > GEEZ hard to keep track! > > Katherine Walters > Histology Director > Central Microscopy Research Facilities > 85 Eckstein Medical Research Building > University of Iowa > Iowa City, Iowa 52242-1101 > > phone: # 319-335-8142 > fax: # 319-384-4469 > > katherine-walters@uiowa.edu > www.uiowa.edu/~cemrf > > > > > -----Original Message----- > From: Victor Tobias [mailto:victor@pathology.washington.edu] > Sent: Tuesday, February 16, 2010 11:37 AM > To: Walters, Katherine S > Cc: Kimberly Tuttle; histonet > Subject: Re: [Histonet] Arcturus Laser Capture Microdissection (LCM) > Systems > > And they have since been bought by.... > > *Danaher* Completes Acquisition > > of *AB SCIEX* and *Molecular > Devices Corporation* (MDCC > ) > 2/1/2010 > > WASHINGTON, Feb. 1 /PRNewswire-FirstCall/ -- Danaher Corporation > announced today that it has completed the previously announced > acquisition of AB SCIEX and Molecular Devices. AB SCIEX is a leading > designer and manufacturer of mass spectrometers, highly sensitive and > sophisticated instruments used by researchers and clinicians to identify > > and quantify specific molecules in complex samples. Molecular Devices > supplies high-performance bio-analytical instrumentation systems and > consumables that accelerate and improve research productivity and > effectiveness in life science research and drug discovery. > > Danaher Corporation > > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > Walters, Katherine S wrote: > > Molecular Devices bought them. Here is their website: > > http://www.moleculardevices.com/pages/instruments/microgenomics.html > > > > Hope this helps, > > Kathy > > > > > > > > Katherine Walters > > Histology Director > > Central Microscopy Research Facilities > > 85 Eckstein Medical Research Building > > University of Iowa > > Iowa City, Iowa 52242-1101 > > > > phone: # 319-335-8142 > > fax: # 319-384-4469 > > > > katherine-walters@uiowa.edu > > www.uiowa.edu/~cemrf > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Kimberly > > Tuttle > > Sent: Tuesday, February 16, 2010 11:16 AM > > To: histonet > > Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) > Systems > > > > Does anyone know who to contact to get this instrument serviced? It > > seems the company is no longer in business. The phone numbers I have > > dont work, and the website is no longer there. I found a company > online > > who repairs them, but they are in Massachusetts, I am in Maryland so > > that would be my last resort. Thanks > > > > Kimberly C. Tuttle HT (ASCP) > > Pathology Biorepository and Research Core > > University of Maryland > > Room NBW58, UMMC > > 22 S. Greene St > > Baltimore, MD 21201 > > (410) 328-5524 > > (410) 328-5508 fax > > Please consider the environment before printing this e-mail. > > > > > > > > > > This e-mail and any accompanying attachments may be privileged, > > confidential, contain protected health information about an identified > > patient or be otherwise protected from disclosure. State and federal > law > > protect the confidentiality of this information. If the reader of this > > message is not the intended recipient; you are prohibited from using, > > disclosing, reproducing or distributing this information; you should > > immediately notify the sender by telephone or e-mail and delete this > > e-mail. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 16 Feb 2010 12:57:11 -0500 > From: "Percival Karen" > Subject: Re: [Histonet] Arcturus Laser Capture Microdissection (LCM) > Systems > To: "histonet" , "Tuttle Kimberly" > > Message-ID: <4B7A962702000011001F8B66@gv01a67m.gv.us.pri.wyeth.com> > Content-Type: text/plain; charset=US-ASCII > > KImberly, > I have an Arcturus Veritas LCM instrument. Contact Molecular Devices at 1 -800-635-5577. I believe they took over Arcturus business and supply matters, although Arcturus still manufactures the instrument. You can also access them online at www.moleculardevices.com > > Karen > > >>> "Kimberly Tuttle" 2/16/2010 12:16 PM >>> > > Does anyone know who to contact to get this instrument serviced? It seems the company is no longer in business. The phone numbers I have dont work, and the website is no longer there. I found a company online who repairs them, but they are in Massachusetts, I am in Maryland so that would be my last resort. Thanks > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > Please consider the environment before printing this e-mail. > > > > > This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 75, Issue 22 > **************************************** From arvidsonkristen <@t> yahoo.com Tue Feb 16 14:02:01 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Feb 16 14:02:03 2010 Subject: [Histonet] Looking to sell xtra inventory Message-ID: <369256.56103.qm@web65706.mail.ac4.yahoo.com> Hello Histonetters, ? I have recently discovered that I have 11 extra boxes of microtome blades we do not use any more.? They are brand new (boxes are sealed) Surgipath low profile Triple Facet blades.? Any takers? From naje1972 <@t> yahoo.com Tue Feb 16 14:56:12 2010 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Tue Feb 16 14:56:16 2010 Subject: [Histonet] Rent-A-Histotech Message-ID: <15849.36080.qm@web110602.mail.gq1.yahoo.com> A group of freelance Histologists seeking temporary assignments. We will come into your hospital, research facility, or/industry that uses histologic techniques and provide histological services, such as, tissue processing,sectioning,and staining. We are experienced histologists well versed in routine staining, special stains,and IHC. We will come into your laboratory when your technologist is vacationing or is sick. We are not a recruiting agency. We are registered Histologists seeking temporary employment. Let us help you with your staffing issues. We have 25-32 years in the field of histology and other related duties. We can be contacted at 312-919-9887. We can also be contacted by e-mail at adminstator@histotechniquesinc.com/cynthiahaynes@histotechniquesinc.com We are located in the Chicago, Illinois area. Thank you CHJames HistoTechniques Inc. From jkiernan <@t> uwo.ca Tue Feb 16 16:00:49 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Feb 16 16:00:55 2010 Subject: [Histonet] Ultralight. Also bigger issues Message-ID: If you don't know what the fixative is, can you justify using it for diagnosis? What will you say when the coroner asks you, "How did you choose which fixative to use?" Will you say "A salesman told me it was better than NBF" or will you say "I used it because our lab has a few books and we checked out the relevant references, discussed the issues and made a decision."? The web site http://www.psl-equip.com/ultralight.php mentions two fixatives: "UL Formalin Fixative" and "UL Zinc Formalin Fixative", with no mention of ingredients other than formaldehyde. They don't even say whether the "formalin fixative" is aqueous, neutral, buffered, alcoholic, etc. Four coloured micrographs illustrate the page. They are pleasing to the eye, but their labels will not inspire confidence in any potential customers who have looked down a microscope and seen smooth muscle (which never has cross-striations), one or more oocytes ("human ova"), or axons in the cerebellar cortex (which are beautiful to behold but cannot be seen at all with H&E). The text alongside the photos insults the intelligence of anyone who might buy for a clinical or research lab. Example: "UltraLight HistologyTM Pristine Artifact Preventer. This is an additive for hematoxylin and eosin stains, which enhances color and extends shelf life up to two months ($$$ saving!!). This product is made to thermodynamically mop up excess formaldehyde (which can induce artifacts which destroy detail) and will improve staining." This is the language of the snake oil salesman. John Kiernan Professor, Dept of Anatomy & Cell Biology University of Western Ontario London, Canada = = = = = = = = ----- Original Message ----- From: Beth Millerman Date: Monday, February 15, 2010 14:20 Subject: [Histonet] Ultralight To: histonet@lists.utsouthwestern.edu > > Felton, > > My lab is Dermatology research and processes skin samples > only. I have > tried the UltraLight Fixative by Pacific Southwest and found > that it works > quite nicely . My H&E are picture perfect, and my > receptors on my IHC's > stain well. I use it because it doesn't seem to be as harsh or > drying as > 10% Formalin. > > Beth Millerman/SWC > Scientist > Stiefel Laboratories, Inc,, a GSK company > Direct Dial; 650-739-2982 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 16 16:11:48 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 16 16:11:56 2010 Subject: [Histonet] Ultralight. Also bigger issues In-Reply-To: Message-ID: <18145.44158.qm@web65716.mail.ac4.yahoo.com> John, you are SO RIGHT! Ren? J. --- On Tue, 2/16/10, John Kiernan wrote: From: John Kiernan Subject: Re: [Histonet] Ultralight. Also bigger issues To: "Beth Millerman" Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, February 16, 2010, 5:00 PM If you don't know what the fixative is, can you justify using it for diagnosis? What will you say when the coroner asks you, "How did you choose which fixative to use?" Will you say "A salesman told me it was better than NBF" or will you say "I used it because our lab has a few books and we checked out the relevant references, discussed the issues and made a decision."? The web site http://www.psl-equip.com/ultralight.php mentions two fixatives: "UL Formalin Fixative" and "UL Zinc Formalin Fixative", with no mention of ingredients other than formaldehyde. They don't even say whether the "formalin fixative" is aqueous, neutral, buffered, alcoholic, etc. Four coloured micrographs illustrate the page. They are pleasing to the eye, but their labels will not inspire confidence in any potential customers who have looked down a microscope and seen smooth muscle (which never has cross-striations), one or more oocytes ("human ova"), or axons in the cerebellar cortex (which are beautiful to behold but cannot be seen at all with H&E). The text alongside the photos insults the intelligence of anyone who might buy for a clinical or research lab. Example: "UltraLight HistologyTM Pristine Artifact Preventer. This is an additive for hematoxylin and eosin stains, which enhances color and extends shelf life up to two months ($$$ saving!!). This product is made to thermodynamically mop up excess formaldehyde (which can induce artifacts which destroy detail) and will improve staining." This is the language of the snake oil salesman. John Kiernan Professor, Dept of Anatomy & Cell Biology University of Western Ontario London, Canada = = = = = = = = ----- Original Message ----- From: Beth Millerman Date: Monday, February 15, 2010 14:20 Subject: [Histonet] Ultralight To: histonet@lists.utsouthwestern.edu > > Felton, > > My lab is Dermatology research and processes skin samples > only. I have > tried the UltraLight Fixative by Pacific Southwest and found > that it works > quite nicely . My H&E are picture perfect, and my > receptors on my IHC's > stain well. I use it because it doesn't seem to be as harsh or > drying as > 10% Formalin. > > Beth Millerman/SWC > Scientist > Stiefel Laboratories, Inc,, a GSK company > Direct Dial; 650-739-2982 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kcai <@t> prosci-inc.com Tue Feb 16 18:37:52 2010 From: kcai <@t> prosci-inc.com (Karen Cai) Date: Tue Feb 16 18:37:55 2010 Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF In-Reply-To: <661401.32335.qm@web65710.mail.ac4.yahoo.com> Message-ID: <000001caaf69$71b38de0$9201a8c0@mic> Hello, Thank you very much for the help. I tried some methods and found that fixation plays major role in my case. 4% PFA followed with ETOH fixation improves. Just want to share my experience here. Best, Karen -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Tuesday, January 12, 2010 12:40 PM To: histonet@lists.utsouthwestern.edu; C.M. van der Loos Cc: kcai@prosci-inc.com; cforster@umn.edu Subject: RE: Eliminating the edge effect in IHC/IF Hi Dr. van der Loos: I had also experienced that artifact caused by less than necessary reagents, but that happens mostly when IHC is done manually, or when the autostainer delivers less amount than required or programmed. IF we assume that the colleague with the question did the IHC manually, your explanation can be accepted, otherwise, poor fixation is a valid cause to the problem. Ren? J. --- On Tue, 1/12/10, C.M. van der Loos wrote: From: C.M. van der Loos Subject: RE: Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Cc: kcai@prosci-inc.com, rjbuesa@yahoo.com, cforster@umn.edu Date: Tuesday, January 12, 2010, 2:54 PM Hi all, We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy. One of my explanations is that the immuno reagents tend to stick to the edges of the tissue section, especially when no Tween20 is involved. As a result the outer edges might become a little dry during incubation times and cause darker mostly non-specific staining. Always try to cover a section completely, including a rim around the section. However, to be honest, I am sure my explanation is certainly not always appropriate. Anyone else???? Cheers, Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 From: Rene J Buesa Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren? J.. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax > www.prosci-inc.com From kcai <@t> prosci-inc.com Tue Feb 16 18:41:11 2010 From: kcai <@t> prosci-inc.com (Karen Cai) Date: Tue Feb 16 18:41:11 2010 Subject: [Histonet] Paraffin tissue slide quality control Message-ID: <000501caaf69$e7d6ee90$9201a8c0@mic> Hello, I am wondering whether anybody having any experience of running the Paraffin tissue slide quality control in order to provide good tissue section? Any important factors? How to avoid wrinkles and folds in the tissue sections? We do get problems on this. Thanks in advance, Best, Karen From mrederick <@t> gmail.com Tue Feb 16 19:00:58 2010 From: mrederick <@t> gmail.com (Michelle Rederick) Date: Tue Feb 16 19:01:02 2010 Subject: [Histonet] Michelle Rederick where did you go? In-Reply-To: <4b6c85511002160742x435997aaib1a120a73f122528@mail.gmail.com> References: <4b6c85511002160742x435997aaib1a120a73f122528@mail.gmail.com> Message-ID: Hello fellow Histonetters! Unfortunately, I am no longer with Milestone but if you should need assistance regarding Milestone's family of products, please contact Jes Strong at 866-955-5300 jes@milestonemed.com. I will continue to be a part of the Histonet family so feel free to contact me at mrederick@gmail.com or 727-204-1910. Best Regards, Michelle On Tue, Feb 16, 2010 at 10:42 AM, Histonet Alias wrote: > *Does anyone have Michelle Rederick's contact info? She was working for > Milestone.* > > > > -- > Al Ias HT(ASCP) > Histology Manager > Pathology Laboratory > United States > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWeems <@t> sjha.org Tue Feb 16 19:06:23 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Feb 16 19:07:38 2010 Subject: [Histonet] Hi to all............. In-Reply-To: <281414D805204848BA1CC3D07AB02D96@Don> References: <281414D805204848BA1CC3D07AB02D96@Don> Message-ID: <27648A6C5BC9B145813DF5182F83AB45CC59@ITSSSXM01V1.one.ads.che.org> Hello Don!! It has been a long time. Good to hear you're enjoying your retirement. Please check in with us now and then. Don't wait so long next time! Take care, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Don Hammer Sent: Monday, February 15, 2010 01:02 To: histonet Subject: [Histonet] Hi to all............. Hi Histology Professionals, I just finished reading the recent issue of "NSH In Action", noting a reference to "Histonet" so thought to drop in to see how this very important, long lasting exchange of information is going. Congrats Linda with thanks to the UTSouthwestern for supporting the site. (14 years now I believe) Perhaps there are a few colleagues still here, continuing to offer assistance from their wealth of experience and knowledge, from back in my Laboratory days (or was that Daze?) :>) It's been near 12 years since retiring, wouldn't give it up for anything, but often think of you all who are in the Profession and now preparing for "Histotechnology Professionals Day" Such a great opportunity to blow your own horn a bit while making the general public aware of your support in the various facets of Medicine. Don't forget to celebrate all you do on a daily basis. I'm completely blown away at how NSH has grown from our Founding Meeting 36 years ago with it's extensive Symposium/Convention, Educational Materials, Journal of Histotechnology, Summer Symposium's, Teleconferences, Specialty Forums, Awards, Positions with or on Boards of National Organizations, just to highlight a few. When viewing the Website containing so much info for Members it's hard to believe much of it was kept on Card files and File drawers manned by one person in the Office. What's difficult to understand is the total Membership is nearly the same number. Well just sending some thoughts. Think I figured out how to post a comment, now I need to go figure out how to view the daily posts on "Histonet" It's been so long since doing that. Don Hammer, Retired Guy " I can't remember the last time I wasn't at least kind of tired." "I totally take back all those times I didn't want to nap when I was younger" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From wingman320 <@t> hotmail.com Tue Feb 16 23:47:00 2010 From: wingman320 <@t> hotmail.com (Harlem Kaputnik) Date: Tue Feb 16 23:47:05 2010 Subject: [Histonet] Paraffin tissue slide quality control In-Reply-To: <000501caaf69$e7d6ee90$9201a8c0@mic> References: <000501caaf69$e7d6ee90$9201a8c0@mic> Message-ID: A good tech will know how to cut good, wrinkle free sections. Making a good section is technique making the ribbon, transferring on the water bath, letting the tissue expand... when to pick it up, when to leave it on the water longer, etc. Some tissues take longer to become wrinkle free, such as intestines, lumens, etc. > From: kcai@prosci-inc.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 16 Feb 2010 16:41:11 -0800 > Subject: [Histonet] Paraffin tissue slide quality control > > Hello, > I am wondering whether anybody having any experience of running the > Paraffin tissue slide quality control in order to provide good tissue > section? Any important factors? How to avoid wrinkles and folds in the > tissue sections? We do get problems on this. > > Thanks in advance, > > Best, > Karen > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/ From MSHERWOOD <@t> PARTNERS.ORG Wed Feb 17 08:49:29 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Feb 17 08:49:53 2010 Subject: [Histonet] Stain for Smooth Muscle Cells Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24039@PHSXMB30.partners.org> Hi all, What stain do most people use to demonstrate smooth muscle cells? I know there are various choices....We are looking at sheep lung. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From jsantiago <@t> bellsouth.net Wed Feb 17 09:04:36 2010 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Wed Feb 17 09:04:41 2010 Subject: [Histonet] Mississippi Society for Histotechnology Message-ID: <57329.27880.qm@web180407.mail.gq1.yahoo.com> Reminder of the upcoming Mississippi Society for Histotechnology Homecoming Event Dates: March 12-14, 2010 Place: Beau Rivage Hotel & Casino, Biloxi, MS Contact: Jerry Santiago at jsantiago@bellsouth.net To register for the meeting, go to the NSH website, then state meetings. Rooms are going quickly, reserve soon. From shive003 <@t> umn.edu Wed Feb 17 09:10:10 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Feb 17 09:10:14 2010 Subject: [Histonet] Stain for Smooth Muscle Cells References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24039@PHSXMB30.partners.org> Message-ID: <98DBD2D680B94660A9D88EFF69B64FCC@auxs.umn.edu> Smooth Muscle Actin; Dako; cat. # M0851, clone 1A4 Requires HIER antigen retrieval (I use a Biocare Decloaking Chamber with Dako Target Retrieval Solution) Strong staining on sheep tissue Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Sherwood, Margaret " To: Sent: Wednesday, February 17, 2010 8:49 AM Subject: Re: [Histonet] Stain for Smooth Muscle Cells Hi all, What stain do most people use to demonstrate smooth muscle cells? I know there are various choices....We are looking at sheep lung. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Feb 17 09:56:06 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Feb 17 09:56:38 2010 Subject: [Histonet] Special stain for Smooth Muscle Cells Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2403A@PHSXMB30.partners.org> Thanks to all who responded, but I was not specific. I am sorry for the confusion. What special stain would you use (i.e. Masson Trichrome, PTAH, etc.) (not IHC) Thanks, again. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rjbuesa <@t> yahoo.com Wed Feb 17 10:22:13 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 17 10:22:17 2010 Subject: [Histonet] Special stain for Smooth Muscle Cells In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2403A@PHSXMB30.partners.org> Message-ID: <424031.13818.qm@web65712.mail.ac4.yahoo.com> Neubert's (1940) is recommended, but a good Masson's trichrome, although not specific for smooth muscle, will show them. It will up to you to recognize the smooth fibers for their location in the subject tissue, and their microscopic characteristics. Uterus will be a good comparison control. Ren? J. --- On Wed, 2/17/10, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] Special stain for Smooth Muscle Cells To: histonet@lists.utsouthwestern.edu Date: Wednesday, February 17, 2010, 10:56 AM Thanks to all who responded, but I was not specific.? I am sorry for the confusion.? What special stain would you use (i.e. Masson Trichrome, PTAH, etc.) (not IHC) Thanks, again. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Feb 17 10:25:36 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Feb 17 10:23:29 2010 Subject: [Histonet] Special stain for Smooth Muscle Cells In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2403A@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2403A@PHSXMB30.partners.org> Message-ID: <4B7C1880.6070003@umdnj.edu> Milligan's Trichrome is reputed to be excellent. I have not used it but colleagues have. Milligan, M. "A trichrome stain for formalin fixed tissue". Am J. Clin. Path., Technical Section 10:184-185, 1946. If you have a copy of Humason's /Animal Tissue Techniques/ the method is there. Geoff Sherwood, Margaret wrote: > Thanks to all who responded, but I was not specific. I am sorry for the > confusion. What special stain would you use (i.e. Masson Trichrome, PTAH, etc.) > (not IHC) > > Thanks, again. > > Peggy > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDW 214) > Massachusetts General Hospital > 55 Fruit Street > Boston, Massachusetts 02114-2696 > 617-724-4839 (voice mail) > 617-726-6983 (lab) > 617-726-1206 (fax) > msherwood@partners.org > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Leiferman <@t> ortho.wisc.edu Wed Feb 17 11:29:11 2010 From: Leiferman <@t> ortho.wisc.edu (Leiferman, Ellen) Date: Wed Feb 17 11:29:19 2010 Subject: [Histonet] IHC Question Message-ID: <117F1D9D-1D6F-42A7-9322-213DB5867298@ortho.wisc.edu> Hi All I?m interested in performing IHC on a mouse ligament. Given their minuscule size, in lieu of paraffin or cryostat embedding and sectioning, we would like to embed in plastic and section at 1 micrometer. Things we are interested in detecting include collagen type 1 and III, CD31, Arginase 1 and iNOS---to name a few. Is there a plastic out there that is recommended for Immuno work? I have performed a brief literature search and have come across Immunobed, JV-4 as well as LR White and Lowicryl. Is anyone familiar with any of these and whether I would have any success? I am familiar with Epon-Araldite embedding and sectioning with glass knives, but have never used any of the above plastics. Thanks for any info From MSHERWOOD <@t> PARTNERS.ORG Wed Feb 17 11:44:51 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Feb 17 11:45:25 2010 Subject: [Histonet] Smooth Muscle Stain Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2403E@PHSXMB30.partners.org> Thanks to all who responded again! We thought Trichrome (Masson)- have used it in the past, but, as you know, there is no "specific" stain just for muscle. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From mpowers <@t> dpspa.com Wed Feb 17 12:35:02 2010 From: mpowers <@t> dpspa.com (Marian Powers) Date: Wed Feb 17 12:35:09 2010 Subject: [Histonet] inform HPV III Family 16 Probe (B) Message-ID: <5d7de0e61002171035n6fb76296nb0f5ee99fdee4c55@mail.gmail.com> Hi: Any one out there running HPV 16 insitu on the Benchmark XT. I need some help with which detection kit to use? Thanks, -- Marian L. Powers, HT(ASCP) Manager, Technical Operations Doctors Pathology Services 1253 College Park Drive Dover, DE 19904 302-677-0000 From TJJ <@t> stowers.org Wed Feb 17 12:54:52 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Wed Feb 17 12:54:59 2010 Subject: [Histonet] Re: IHC Question Message-ID: Dear Ellen, You wrote: "I'm interested in performing IHC on a mouse ligament. Given their minuscule size, in lieu of paraffin or cryostat embedding and sectioning, we would like to embed in plastic and section at 1 micrometer. Things we are interested in detecting include collagen type 1 and III, CD31, Arginase 1 and iNOS---to name a few. Is there a plastic out there that is recommended for Immuno work? I have performed a brief literature search and have come across Immunobed, JV-4 as well as LR White and Lowicryl. Is anyone familiar with any of these and whether I would have any success? I am familiar with Epon-Araldite embedding and sectioning with glass knives, but have never used any of the above plastics. Thanks for any info" I think you have a typo, it shoudl be JB-4 and not JV-4. JB-4 is not suitable for IHC. Any Methyl methacrylate or polymethyl methacrylate formulas should work for you. In your list, Immunobed, LR White, and Lowicryl would all be suitable. Sectioning and mounting these are different from usual mounting methods. You will need to wet the block face with alcohol for each section. You will put the cut section on a glass slide flooded with alcohol and stretch it, pulling out wrinkles. It will need to be covered with a plastic film, rolled with a roller, and put into a press of some kind and dried overnight. It helps to watch someone else do this first before you try doing it on your own. We have limited experience with this. Perhaps one of the hard tissue folks will chime in here with some great resource to help you. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From marktarango <@t> gmail.com Wed Feb 17 12:58:08 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Feb 17 12:58:12 2010 Subject: [Histonet] inform HPV III Family 16 Probe (B) In-Reply-To: <5d7de0e61002171035n6fb76296nb0f5ee99fdee4c55@mail.gmail.com> References: <5d7de0e61002171035n6fb76296nb0f5ee99fdee4c55@mail.gmail.com> Message-ID: <5b6eb13e1002171058y46fb7d54vdb20298e8df8fbbb@mail.gmail.com> The inform HPV16 is a DNP labeled probe. You should use the ISH Iview plus detection kit for staining. Mark Tarango HT(ASCP)QIHC On Wed, Feb 17, 2010 at 10:35 AM, Marian Powers wrote: > Hi: Any one out there running HPV 16 insitu on the Benchmark XT. I need > some help with which detection kit to use? > > Thanks, > > > > -- > Marian L. Powers, HT(ASCP) > Manager, Technical Operations > > Doctors Pathology Services > 1253 College Park Drive > Dover, DE 19904 > 302-677-0000 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Pat.Bell <@t> ucdenver.edu Wed Feb 17 13:41:11 2010 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Wed Feb 17 13:41:16 2010 Subject: [Histonet] QIHC In-Reply-To: <4B790FC5.D886.00F4.3@umm.edu> References: <4B790FC5.D886.00F4.3@umm.edu> Message-ID: <64DB27005E2FD3439E88502D7A5C91218F584E208E@CORTEZ.ucdenver.pvt> I tried to email Ethel at this email address and was told that it was not a working email address and was "undeliverable". Also, what NSH study guide did you use? Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Monday, February 15, 2010 7:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC I recently took and passed the examination . I used the Dako book, material from Ethel Macrea's presentation, NSH study guide and material from the other references on the list. Ethel can be reached at emacrea@swskinpath.com She is a wonderful resource. Message: 6 Date: Fri, 12 Feb 2010 20:22:01 -0500 From: jfi786@aol.com Subject: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu Message-ID: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Feb 17 14:24:34 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Feb 17 14:24:45 2010 Subject: [Histonet] QIHC In-Reply-To: <64DB27005E2FD3439E88502D7A5C91218F584E208E@CORTEZ.ucdenver.pvt> Message-ID: Pat, The link below will take you to the Self-Assessment Books by the NSH. #6 covers Immunohistochemistry. It is $15 for NSH members and $35 for non members. You will also find other resources available at the NSH website. Histotechnology: A Self Instructional Text, 3rd Edition (Carson and Hladik) has an updated chapter (chpt 12) on immunochemistry. Also keep your eye open for state meetings that are offering QIHC review, or the NSH symposium in the summer or fall. The California Society has their meeting in May and Ethel Macrae is presenting "Preparing for the QIHC. The program will be available shortly at http://www.californiahistology.org/ The ASCP also has some on-line courses related to IHC. http://www.ascp.org/LongDescriptions/e-HistoLogic-Online-Histology-Education.aspx http://www.nsh.org/content/preparing-certification-exams Jennifer MacDonald "Bell, Pat" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/17/2010 11:45 AM To 'Walter Benton' , "histonet@lists.utsouthwestern.edu" cc Subject RE: [Histonet] QIHC I tried to email Ethel at this email address and was told that it was not a working email address and was "undeliverable". Also, what NSH study guide did you use? Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Monday, February 15, 2010 7:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC I recently took and passed the examination . I used the Dako book, material from Ethel Macrea's presentation, NSH study guide and material from the other references on the list. Ethel can be reached at emacrea@swskinpath.com She is a wonderful resource. Message: 6 Date: Fri, 12 Feb 2010 20:22:01 -0500 From: jfi786@aol.com Subject: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu Message-ID: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Montina.VanMeter <@t> pbrc.edu Wed Feb 17 14:33:10 2010 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Wed Feb 17 14:46:00 2010 Subject: [Histonet] QIHC In-Reply-To: <64DB27005E2FD3439E88502D7A5C91218F584E208E@CORTEZ.ucdenver.pvt> References: <4B790FC5.D886.00F4.3@umm.edu> <64DB27005E2FD3439E88502D7A5C91218F584E208E@CORTEZ.ucdenver.pvt> Message-ID: <4FE7FB862E90E448AE32388E759220E501FE4BA9@pbrcas31.pbrc.edu> Here is Ethel's contact information: Ethel R. Macrea, HT(ASCP)QIHC Ventana Medical Systems HC 3 Box 1023, 22191 S. Branch Road Tucson, AZ 85739 UNITED STATES Phone: (520)219-8471 Fax: (502)219-8454 Email: dawnbrok@earthlink.net Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Pat Sent: Wednesday, February 17, 2010 1:41 PM To: 'Walter Benton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] QIHC I tried to email Ethel at this email address and was told that it was not a working email address and was "undeliverable". Also, what NSH study guide did you use? Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Monday, February 15, 2010 7:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC I recently took and passed the examination . I used the Dako book, material from Ethel Macrea's presentation, NSH study guide and material from the other references on the list. Ethel can be reached at emacrea@swskinpath.com She is a wonderful resource. Message: 6 Date: Fri, 12 Feb 2010 20:22:01 -0500 From: jfi786@aol.com Subject: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu Message-ID: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Bell <@t> ucdenver.edu Wed Feb 17 15:05:02 2010 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Wed Feb 17 15:05:07 2010 Subject: [Histonet] QIHC In-Reply-To: <4FE7FB862E90E448AE32388E759220E501FE4BA9@pbrcas31.pbrc.edu> References: <4B790FC5.D886.00F4.3@umm.edu> <64DB27005E2FD3439E88502D7A5C91218F584E208E@CORTEZ.ucdenver.pvt> <4FE7FB862E90E448AE32388E759220E501FE4BA9@pbrcas31.pbrc.edu> Message-ID: <64DB27005E2FD3439E88502D7A5C91218F584E2094@CORTEZ.ucdenver.pvt> Thank you to everyone for all of your help. I appreciate it very much. Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: Montina Van Meter [mailto:Montina.VanMeter@pbrc.edu] Sent: Wednesday, February 17, 2010 1:33 PM To: Bell, Pat; Walter Benton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] QIHC Here is Ethel's contact information: Ethel R. Macrea, HT(ASCP)QIHC Ventana Medical Systems HC 3 Box 1023, 22191 S. Branch Road Tucson, AZ 85739 UNITED STATES Phone: (520)219-8471 Fax: (502)219-8454 Email: dawnbrok@earthlink.net Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Pat Sent: Wednesday, February 17, 2010 1:41 PM To: 'Walter Benton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] QIHC I tried to email Ethel at this email address and was told that it was not a working email address and was "undeliverable". Also, what NSH study guide did you use? Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Monday, February 15, 2010 7:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC I recently took and passed the examination . I used the Dako book, material from Ethel Macrea's presentation, NSH study guide and material from the other references on the list. Ethel can be reached at emacrea@swskinpath.com She is a wonderful resource. Message: 6 Date: Fri, 12 Feb 2010 20:22:01 -0500 From: jfi786@aol.com Subject: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu Message-ID: <8CC7A6213F16820-47FC-8816@webmail-m018.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Feb 18 08:05:13 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Feb 18 08:05:21 2010 Subject: [Histonet] reticulin stain Gomori Message-ID: <5CCBC005A7C240C6B75181BB8F0F18F1@dielangs.at> Hi all! Today I was asked if I knew the chemistry behind the ammonia-iron-sulfat NH4Fe(SO4)2 . 12H2O reaction in the reticulin stain of Gomori. I couldn't help so I forward the question to the histonet comunity. I hope, someone has a suggestion for me. Gudrun Lang From mtitford <@t> aol.com Thu Feb 18 11:58:21 2010 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Feb 18 11:58:42 2010 Subject: [Histonet] Gomori Reticulum Message-ID: <8CC7EDB17B7B811-2FE0-217D@webmail-d077.sysops.aol.com> Gudrun asks about how the Gomori retic procedure works: It is my understanding that the iron alum (ferric ammonium sulphate) acts as a sensitizer. The iron combines with the aldehyde formed in the reticulum from the previous oxidation step to create an aldehyde-metal complex. In the third step with the ammoniacal silver nitrate, the silver combines with this complex, but is not visible. Treatment with formlin makes it visible.Toning with gold chloride stabilises the silver, and thiosulphate removes excess silver remaining in the tissue. We all like to say "Reticulin" but "Reticulum" is the correct word. Michael Titford USA Pathology Mobile AL USA = From cbarone <@t> NEMOURS.ORG Thu Feb 18 12:23:32 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Thu Feb 18 12:23:36 2010 Subject: [Histonet] DRGs Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7A0D@wlmmsx01.nemours.org> Histonetter's...we received a "boat-load" of mouse DRGs that had been prepared in histogel and are cutting...well..not so good. We normally do DRGs from FS and get beautiful results. We have used histogel before with other small sample and have never had issues... not sure if it is the Histogel or DRG's (fixed in 10% NBF and then transferred to 70% before placed into the histogel).is the issue..I seem to remember that histogel requires formalin and wonder if the transfer to 70% is causing our problem ...but, obviously there is not much room for error with such tiny- tiny samples and they are already process and in paraffin? I am not quite sure how twe can improve the ones that came in histogel, and were processed to paraffin a paraffin block....any idea's? any experience? any anything? Thx- ASAP! cbarone@nemours.org From portera <@t> msu.edu Thu Feb 18 12:30:59 2010 From: portera <@t> msu.edu (Amy Porter) Date: Thu Feb 18 12:36:05 2010 Subject: [Histonet] DRGs References: <37E4BAC017F57141AF64FAA5AEB04CE8033A7A0D@wlmmsx01.nemours.org> Message-ID: <06B1298240A445D7BA88BB4A86DAB781@histolab> We normally do CG's and DRG's in Frozen format as well and have had difficulty working with histogel and low percetages of ethanol. The histogel doesn't really bond with the samples very well when taken from an low % ethanol for us. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Barone, Carol " To: Sent: Thursday, February 18, 2010 1:23 PM Subject: [Histonet] DRGs Histonetter's...we received a "boat-load" of mouse DRGs that had been prepared in histogel and are cutting...well..not so good. We normally do DRGs from FS and get beautiful results. We have used histogel before with other small sample and have never had issues... not sure if it is the Histogel or DRG's (fixed in 10% NBF and then transferred to 70% before placed into the histogel).is the issue..I seem to remember that histogel requires formalin and wonder if the transfer to 70% is causing our problem ...but, obviously there is not much room for error with such tiny- tiny samples and they are already process and in paraffin? I am not quite sure how twe can improve the ones that came in histogel, and were processed to paraffin a paraffin block....any idea's? any experience? any anything? Thx- ASAP! cbarone@nemours.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jennifer.Bull <@t> northwestpathology.com Thu Feb 18 13:35:29 2010 From: Jennifer.Bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Thu Feb 18 13:35:44 2010 Subject: [Histonet] Parvovirus Control Slides Message-ID: <85760CECEC18444BB95F26D5E88DAEAA227F91F391@hinet2.hinet.org> Does anyone out there have a resource for unstained slides or tissue infected with parvovirus? Thanks Jennifer Bull Northwest Pathology Bellingham, WA 98225 w: 360-734-2800 x503 jennifer.bull@nwpathology.com mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- From portera <@t> msu.edu Thu Feb 18 13:52:20 2010 From: portera <@t> msu.edu (Amy Porter) Date: Thu Feb 18 13:52:26 2010 Subject: [Histonet] Parvovirus Control Slides References: <85760CECEC18444BB95F26D5E88DAEAA227F91F391@hinet2.hinet.org> Message-ID: <945956848F934590B434FD6AE7DFC074@histolab> Jennifer - could you contact me off list for what your actual need is. Thanks - Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Bull, Jennifer L." To: Sent: Thursday, February 18, 2010 2:35 PM Subject: [Histonet] Parvovirus Control Slides Does anyone out there have a resource for unstained slides or tissue infected with parvovirus? Thanks Jennifer Bull Northwest Pathology Bellingham, WA 98225 w: 360-734-2800 x503 jennifer.bull@nwpathology.com mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bitesizellama <@t> gmail.com Thu Feb 18 17:00:53 2010 From: bitesizellama <@t> gmail.com (Mauricio Avigdor) Date: Thu Feb 18 17:00:57 2010 Subject: [Histonet] IF staining on peritoneal macrophages Message-ID: <1b2831cd1002181500r2b6e1066s51c002b619c6fa28@mail.gmail.com> Greetings all, I am trying to do immunofluorescence on peritoneal macrophages. I am having a couple of issues that I was hoping one of you could help me resolve. Firstly, I am having uneven results with the Cytospin. Cells tend to get washed off the slides during rinses. Does anyone have tips on how to make cells stick a little better to Cytoslides? Secondly, I have not yet found a satisfactory method for fixation. In order to prevent loss of cells when dipping the slides into fixatives, I am having to air dry the slides before I can do anything to them. I tried the Shandon Collection Fluid (ethanol, isopropanol, carbowax) with great success, but it killed the fluorescence. I am leaning towards spinning the lavage fluid until I get a pellet and then resuspending in PBS with a little BSA added. I hope this makes cells stick well enough that I can put the slides in formalin or acetone. Any thoughts are appreciated! From anonwums1 <@t> gmail.com Thu Feb 18 18:41:25 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Feb 18 18:41:32 2010 Subject: [Histonet] IF staining on peritoneal macrophages In-Reply-To: <1b2831cd1002181500r2b6e1066s51c002b619c6fa28@mail.gmail.com> References: <1b2831cd1002181500r2b6e1066s51c002b619c6fa28@mail.gmail.com> Message-ID: <858249121002181641n17ecfe79j7545e9bef7097504@mail.gmail.com> The best way I know to get cells to stick is to first cytospin liquid with some protein (BSA or serum) onto the filters to pre-wet them. After that's done, cytospin your cells. I've gotten this to work with fairly rare FACS sorted cells. I've also found that the smaller volume the cells are in, the better your cytospin. Ideally is 100 - 200 uL. For FACS sorted cells, this means you want to sort into as small a volume as possible. For lavage cells, that may mean spinning them down first, although for FACS cells, the cells are fragile and don't like being centrifuged. I've gotten cells to stick to the slides by circling the cells with a PAP pen and then just putting fixative inside PAP while the slides are lying horizontally. Adam On Thu, Feb 18, 2010 at 5:00 PM, Mauricio Avigdor wrote: > Greetings all, > > I am trying to do immunofluorescence on peritoneal macrophages. I am having > a couple of issues that I was hoping one of you could help me resolve. > > Firstly, I am having uneven results with the Cytospin. Cells tend to get > washed off the slides during rinses. Does anyone have tips on how to make > cells stick a little better to Cytoslides? Secondly, I have not yet found a > satisfactory method for fixation. In order to prevent loss of cells when > dipping the slides into fixatives, I am having to air dry the slides before > I can do anything to them. I tried the Shandon Collection Fluid (ethanol, > isopropanol, carbowax) with great success, but it killed the fluorescence. > > I am leaning towards spinning the lavage fluid until I get a pellet and > then > resuspending in PBS with a little BSA added. I hope this makes cells stick > well enough that I can put the slides in formalin or acetone. > > Any thoughts are appreciated! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaylundgren <@t> gmail.com Thu Feb 18 23:27:54 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Feb 18 23:28:02 2010 Subject: [Histonet] IF staining on peritoneal macrophages In-Reply-To: <1b2831cd1002181500r2b6e1066s51c002b619c6fa28@mail.gmail.com> References: <1b2831cd1002181500r2b6e1066s51c002b619c6fa28@mail.gmail.com> Message-ID: Mauricio, Sorry if this is too obvious, but are you using plus slides? I'm not sure what you mean by "cytoslides". I always use plus slides with the Cytospin, and they stay on through H&E, Pap, and DQ, although I'm not trying IF on them. Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Fri Feb 19 07:30:53 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Feb 19 07:31:41 2010 Subject: [Histonet] FITC for IgG 1,2,3,4 Message-ID: <61135F0455D33347B5AAE209B903A30433DEB6B4@EXCHVS2.medctr.ad.wfubmc.edu> I have a request from a pathologist for these antibodies. I have been unable to locate a vendor in my usual places. Is anyone doing these antibodies, and if so, could you let me know a vendor (s) to check out? Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 From bitesizellama <@t> gmail.com Fri Feb 19 09:47:43 2010 From: bitesizellama <@t> gmail.com (Mauricio Avigdor) Date: Fri Feb 19 09:47:48 2010 Subject: [Histonet] IF staining on peritoneal macrophages Message-ID: <1b2831cd1002190747v5a6e77d4weabb4c40497cb706@mail.gmail.com> Thank you Adam and Jay for your replies. Cytoslides are the pre-marked slides for use with the Cytospin - http://www.thermo.com/com/cda/product/detail/0,1055,21035,00.html Adam, do you have a recommended concentration of BSA for this? Also, do you air dry the slides prior to fixation? Do you air dry afterwards? Thanks again for your help. Mauricio From anonwums1 <@t> gmail.com Fri Feb 19 10:31:20 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Fri Feb 19 10:31:36 2010 Subject: [Histonet] IF staining on peritoneal macrophages In-Reply-To: <1b2831cd1002190747v5a6e77d4weabb4c40497cb706@mail.gmail.com> References: <1b2831cd1002190747v5a6e77d4weabb4c40497cb706@mail.gmail.com> Message-ID: <858249121002190831q463de525l7c2dbee4f30f8007@mail.gmail.com> When I did it, I think I used our standard sort buffer, which is 0.2% BSA in PBS pH 7.4. I've also read that you can use anything with serum, typically 5-10%. The key seems to be prewetting the membranes with something with protein. I usually cytospin them and briefly (30 seconds) air dry them until there is no obvious liquid, before I added the fixative. I managed to get usable (but not ideal) cytospins from as few as 1000 cells. Let me know if it works, Adam On Fri, Feb 19, 2010 at 9:47 AM, Mauricio Avigdor wrote: > Thank you Adam and Jay for your replies. > > Cytoslides are the pre-marked slides for use with the Cytospin - > http://www.thermo.com/com/cda/product/detail/0,1055,21035,00.html > > Adam, do you have a recommended concentration of BSA for this? Also, do you > air dry the slides prior to fixation? Do you air dry afterwards? Thanks > again for your help. > > Mauricio > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wlecorch <@t> rwjuhh.edu Fri Feb 19 12:17:51 2010 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Fri Feb 19 12:17:58 2010 Subject: [Histonet] IF staining on peritoneal macrophages Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71A4AB78B43@HAMEXMBA.rwjham.local> You may want to try using the "NuView" Cyto prep technique from QC sciences it is a good alternative to a Cytospin From Dorothy.L.Webb <@t> HealthPartners.Com Fri Feb 19 12:29:01 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Feb 19 12:29:08 2010 Subject: [Histonet] EDTA Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C57696D718@HPEMX3.HealthPartners.int> I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. Thank you ahead of time for your advice! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From rsrichmond <@t> gmail.com Fri Feb 19 13:07:37 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Feb 19 13:07:44 2010 Subject: [Histonet] Re: DRGs Message-ID: DRG's for mice? Do mice have Medicare now? I hope the pending health care reform legislation addresses this issue - it's no wonder costs are spiraling out of sight! I have the feeling that a mouse DRG might be something other than "Diagnosis Related Group", the coding scheme that has determined Medicare (and many other insurers') payments to hospitals for close to thirty years now. Histonet includes many people from diverse disciplines. It's better not to assume that any but the commonest abbreviations (H & E and not too many more) will be understood by every Histonetter. Bob Richmond Samurai Pathologist Knoxville TN (I mean Tennessee) From rjbuesa <@t> yahoo.com Fri Feb 19 15:03:27 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 19 15:03:32 2010 Subject: [Histonet] EDTA In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43C57696D718@HPEMX3.HealthPartners.int> Message-ID: <796184.91241.qm@web65714.mail.ac4.yahoo.com> EDTA (which stands for Ethylene-diamino-tratacetic-acid) is a chelating agent and the method of choice to decalcify BM biopsies. The thing is that it should be used alone, and not combined with any acid, as you state (not even formic acid). By itself will produce an extremely gentle decalcification that will be completely suitable for IHC studies. Ren? J. --- On Fri, 2/19/10, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] EDTA To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, February 19, 2010, 1:29 PM I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix.? I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. Thank you ahead of time for your advice! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jphistology <@t> gmail.com Fri Feb 19 15:21:09 2010 From: jphistology <@t> gmail.com (Joao Pessoa) Date: Fri Feb 19 15:21:14 2010 Subject: [Histonet] Tissue repository Message-ID: <9e4d12d1002191321o6a98e4f6o481f75b6c603c393@mail.gmail.com> Dear histonet, Does anyone know where I can find a tissue repository to exchange tissue blocks? For example, I have some extra melanoma FFPE tissue blocks but would like some normal FFPE tonsil tissue blocks. I do not wish to buy blocks, but rather trade. Please let me know if there is such a place. Thanks, Joao Histo Tech From rjbuesa <@t> yahoo.com Fri Feb 19 15:25:07 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 19 15:25:12 2010 Subject: [Histonet] Tissue repository In-Reply-To: <9e4d12d1002191321o6a98e4f6o481f75b6c603c393@mail.gmail.com> Message-ID: <464358.67154.qm@web65702.mail.ac4.yahoo.com> Contact the NSH that I think offers such a service. Ren? J. --- On Fri, 2/19/10, Joao Pessoa wrote: From: Joao Pessoa Subject: [Histonet] Tissue repository To: histonet@lists.utsouthwestern.edu Date: Friday, February 19, 2010, 4:21 PM Dear histonet, Does anyone know where I can find a tissue repository to exchange tissue blocks?? For example, I have some extra melanoma FFPE tissue blocks but would like some normal FFPE tonsil tissue blocks.? I do not wish to buy blocks, but rather trade.? Please let me know if there is such a place. Thanks, Joao Histo Tech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pathrm35 <@t> comcast.net Fri Feb 19 16:03:50 2010 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Fri Feb 19 16:03:52 2010 Subject: [Histonet] seeking histology position Message-ID: <114145463.6366871266617030451.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Fellow techs, I am currently seeking?a F/T position as a bench tech. I am ASCP certified and Florida licensed w/ a BS degree.?Relocation and temp. positions are a possibility. Please feel free to pass this e mail along if you know of anyone looking for a tech. Thanks in advance Ron Martin From gayle.callis <@t> bresnan.net Fri Feb 19 16:03:46 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Feb 19 16:03:55 2010 Subject: [Histonet] Re: EDTA Message-ID: <000101cab1af$698cf8c0$3ca6ea40$@callis@bresnan.net> You wrote: I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. **************************************************************************** **************************************************************************** ** The purpose of EDTA in an acid decalcifier is probably NOT performing the actual removal of calcium (or very little) from the bone but chelating calcium ionized from bone by the acid that then (the calcium) settles to the bottom of the container. I doubt very much that you will be dealing with EDTA but with formic acid decalcification. People who have developed acid decalcifying solutions, with either formic acid or hydrochloric acid tout that the bone section looks very good, better that if EDTA is not present in the solution. I never tested this claim in our bone studies, but it might be something to try, an acid solution containing EDTA and one without EDTA (keeping the same kind of acid in the study). The acid is going to ionize the calcium from bone at a faster rate than the EDTA can chelate the calcium. But most importantly, EDTA does not work in a low pH environment. The chemistry of how EDTA acts at different pHs is well documented by chemists, and found in book chapters on EDTA. My physical chemist spouse supplied me with such a chapter. The pH of formic acid is what, pH 3 or so, and if so, EDTA only begins to chelate calcium around pH 4, and when fully protonated at pH 8, decalcifies faster at that pH than at pH 4, or below pH7. The working pH for most EDTA solutions is commonly 7 to 7.6, but when the pH becomes more alkaline ( and 8 is going into alkaline range) then alkaline sensitive protein linkages can be damaged. EDTA, correctly written out by Rene Buesa e.g. ethylene diaminatetraacetiic acid comes in several molecular weights depending on whether is it EDTA without attached sodium, EDTA disodium and EDTA tetrasodium. Tetrasodium is very soluble in water or PBS, but has a very alkaline pH that requires adjustment down to pH 7 to 7.6 or that high pH will damage alkaline sensitive protein linkages. EDTA and disodium EDTA are not as soluble, usually no more than a 10% solution, requiring heat or addition of sodium hydroxide in order to dissolve. EDTA is also expensive, not affected by heat up to 60C but only if the bone is totally fixed with NBF. I would not advise using 60C during lengthy EDTA decalcification, and possible damage to heat labile antigens. There are publications on using EDTA to decalcify fresh bone samples, then snap freeze, cut frozen sections, then acetone fix the sections for murine CD markers compromised by both NBF and acid decalcification. EDTA mixtures are found in Histonet Archives, histotechnology books and on websites e.g. IHCworld, as a chelating agent, it is a very slow decalcifier. More advantages of EDTA are - no damage to antigens, nucleic acid staining, nor other tissue components. It does affect the enzyme histochemical stain for alkaline phosphatase, and magnesium ions (chelated by EDTA) must be replaced in the staining solution. Most clinical laboratories prefer to use formic acid or hydrochloric acid decalcifying solutions instead of EDTA when rapid diagnosis is needed. If you try EDTA alone, rinse the bone well (after decalcification) with running tap water since residual, excess EDTA in tissue precipitates in presence of alcohol, and the ppt makes the tissue difficult to section. Endpoint determinations for complete removal of calcium with EDTA cannot be done chemically, although there is a weight gain/weight loss method that we use routinely that works or use an Xray machine, Faxitron. You can buy EDTA mixtures from Poly Scientific and some other suppliers, or make it up yourself. Otherwise, try the formic acid with EDTA, it may work very well for you while not sacrificing speed for diagnosis. Formic acid will be less damaging to antigens than hydrochloric acid, and is a popular decalcifier because it is gentler than HCl. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 From Barry.R.Rittman <@t> uth.tmc.edu Fri Feb 19 16:20:25 2010 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Feb 19 16:20:30 2010 Subject: [Histonet] Re: EDTA In-Reply-To: <000101cab1af$698cf8c0$3ca6ea40$%callis@bresnan.net> References: <000101cab1af$698cf8c0$3ca6ea40$%callis@bresnan.net> Message-ID: <75A0543E23D3A7458012D9E02EDBEC00098CEE3112@UTHCMS1.uthouston.edu> I agree with Gayle and Rene I would not even use a mixture of the two. Formic acid demineralization will work fine, the theory of using EDTA with it makes no sense. EDTA demineralization usually uses the disodium salt. EDTA by itself is not very soluble but its sodium salts are. It can be present as mono-, di-, tri- or tetra-sodium salts depending on the pH. At pH around 11 there are 4 groups that can be replaced with for example calcium. Usually the disodium salt of EDTA is used as the tri and tetrasodium salts are only present in very alkaline solutions that tend to cause tissue maceration. At acidic pH the EDTA will only work slowly and have one group replaceable as the monosodium EDTA. There are several good formulae for using EDTA and formic acid in separate formulae, depending on your specific needs.. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis [gayle.callis@bresnan.net] Sent: Friday, February 19, 2010 4:03 PM To: 'Histonet' Subject: [Histonet] Re: EDTA You wrote: I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. **************************************************************************** **************************************************************************** ** The purpose of EDTA in an acid decalcifier is probably NOT performing the actual removal of calcium (or very little) from the bone but chelating calcium ionized from bone by the acid that then (the calcium) settles to the bottom of the container. I doubt very much that you will be dealing with EDTA but with formic acid decalcification. People who have developed acid decalcifying solutions, with either formic acid or hydrochloric acid tout that the bone section looks very good, better that if EDTA is not present in the solution. I never tested this claim in our bone studies, but it might be something to try, an acid solution containing EDTA and one without EDTA (keeping the same kind of acid in the study). The acid is going to ionize the calcium from bone at a faster rate than the EDTA can chelate the calcium. But most importantly, EDTA does not work in a low pH environment. The chemistry of how EDTA acts at different pHs is well documented by chemists, and found in book chapters on EDTA. My physical chemist spouse supplied me with such a chapter. The pH of formic acid is what, pH 3 or so, and if so, EDTA only begins to chelate calcium around pH 4, and when fully protonated at pH 8, decalcifies faster at that pH than at pH 4, or below pH7. The working pH for most EDTA solutions is commonly 7 to 7.6, but when the pH becomes more alkaline ( and 8 is going into alkaline range) then alkaline sensitive protein linkages can be damaged. EDTA, correctly written out by Rene Buesa e.g. ethylene diaminatetraacetiic acid comes in several molecular weights depending on whether is it EDTA without attached sodium, EDTA disodium and EDTA tetrasodium. Tetrasodium is very soluble in water or PBS, but has a very alkaline pH that requires adjustment down to pH 7 to 7.6 or that high pH will damage alkaline sensitive protein linkages. EDTA and disodium EDTA are not as soluble, usually no more than a 10% solution, requiring heat or addition of sodium hydroxide in order to dissolve. EDTA is also expensive, not affected by heat up to 60C but only if the bone is totally fixed with NBF. I would not advise using 60C during lengthy EDTA decalcification, and possible damage to heat labile antigens. There are publications on using EDTA to decalcify fresh bone samples, then snap freeze, cut frozen sections, then acetone fix the sections for murine CD markers compromised by both NBF and acid decalcification. EDTA mixtures are found in Histonet Archives, histotechnology books and on websites e.g. IHCworld, as a chelating agent, it is a very slow decalcifier. More advantages of EDTA are - no damage to antigens, nucleic acid staining, nor other tissue components. It does affect the enzyme histochemical stain for alkaline phosphatase, and magnesium ions (chelated by EDTA) must be replaced in the staining solution. Most clinical laboratories prefer to use formic acid or hydrochloric acid decalcifying solutions instead of EDTA when rapid diagnosis is needed. If you try EDTA alone, rinse the bone well (after decalcification) with running tap water since residual, excess EDTA in tissue precipitates in presence of alcohol, and the ppt makes the tissue difficult to section. Endpoint determinations for complete removal of calcium with EDTA cannot be done chemically, although there is a weight gain/weight loss method that we use routinely that works or use an Xray machine, Faxitron. You can buy EDTA mixtures from Poly Scientific and some other suppliers, or make it up yourself. Otherwise, try the formic acid with EDTA, it may work very well for you while not sacrificing speed for diagnosis. Formic acid will be less damaging to antigens than hydrochloric acid, and is a popular decalcifier because it is gentler than HCl. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Feb 19 17:32:13 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Feb 19 17:32:23 2010 Subject: [Histonet] Re: EDTA In-Reply-To: <75A0543E23D3A7458012D9E02EDBEC00098CEE3112@UTHCMS1.uthouston.edu> References: <000101cab1af$698cf8c0$3ca6ea40$%callis@bresnan.net> <75A0543E23D3A7458012D9E02EDBEC00098CEE3112@UTHCMS1.uthouston.edu> Message-ID: <000301cab1bb$c4e91210$4ebb3630$@callis@bresnan.net> We use EDTA tetra-sodium salt, pH 7.6 but the pH is adjusted down with glacial acetic acid using continuous stirring and a pH electrode in the solution (titration with the acid). This is a recipe from a Dr. Webster (Webb) S. S. Jee publication. We like this since this 14% EDTA dissolves immediately in water or PBS compared disodium EDTA, and at a higher concentration = more molecules of EDTA available. We are very careful to NOT use this 14% tetrasodium EDTA at it original pH of around 10 or more. Decalcification is still very slow, but a recent bone project required EDTA decalcification (so we thought!) to protect antigens never stained before in our lab. After trying both the EDTA and 10% formic acid, we found the antigen to be very robust after formic acid decalcification too. Lesson learned, don't put off a pilot study to test IHC after acid decalcification. The formic acid would have been faster and cheaper. Gayle Callis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Friday, February 19, 2010 3:20 PM To: 'Histonet' Subject: RE: [Histonet] Re: EDTA I agree with Gayle and Rene I would not even use a mixture of the two. Formic acid demineralization will work fine, the theory of using EDTA with it makes no sense. EDTA demineralization usually uses the disodium salt. EDTA by itself is not very soluble but its sodium salts are. It can be present as mono-, di-, tri- or tetra-sodium salts depending on the pH. At pH around 11 there are 4 groups that can be replaced with for example calcium. Usually the disodium salt of EDTA is used as the tri and tetrasodium salts are only present in very alkaline solutions that tend to cause tissue maceration. At acidic pH the EDTA will only work slowly and have one group replaceable as the monosodium EDTA. There are several good formulae for using EDTA and formic acid in separate formulae, depending on your specific needs.. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis [gayle.callis@bresnan.net] Sent: Friday, February 19, 2010 4:03 PM To: 'Histonet' Subject: [Histonet] Re: EDTA You wrote: I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. **************************************************************************** **************************************************************************** ** The purpose of EDTA in an acid decalcifier is probably NOT performing the actual removal of calcium (or very little) from the bone but chelating calcium ionized from bone by the acid that then (the calcium) settles to the bottom of the container. I doubt very much that you will be dealing with EDTA but with formic acid decalcification. People who have developed acid decalcifying solutions, with either formic acid or hydrochloric acid tout that the bone section looks very good, better that if EDTA is not present in the solution. I never tested this claim in our bone studies, but it might be something to try, an acid solution containing EDTA and one without EDTA (keeping the same kind of acid in the study). The acid is going to ionize the calcium from bone at a faster rate than the EDTA can chelate the calcium. But most importantly, EDTA does not work in a low pH environment. The chemistry of how EDTA acts at different pHs is well documented by chemists, and found in book chapters on EDTA. My physical chemist spouse supplied me with such a chapter. The pH of formic acid is what, pH 3 or so, and if so, EDTA only begins to chelate calcium around pH 4, and when fully protonated at pH 8, decalcifies faster at that pH than at pH 4, or below pH7. The working pH for most EDTA solutions is commonly 7 to 7.6, but when the pH becomes more alkaline ( and 8 is going into alkaline range) then alkaline sensitive protein linkages can be damaged. EDTA, correctly written out by Rene Buesa e.g. ethylene diaminatetraacetiic acid comes in several molecular weights depending on whether is it EDTA without attached sodium, EDTA disodium and EDTA tetrasodium. Tetrasodium is very soluble in water or PBS, but has a very alkaline pH that requires adjustment down to pH 7 to 7.6 or that high pH will damage alkaline sensitive protein linkages. EDTA and disodium EDTA are not as soluble, usually no more than a 10% solution, requiring heat or addition of sodium hydroxide in order to dissolve. EDTA is also expensive, not affected by heat up to 60C but only if the bone is totally fixed with NBF. I would not advise using 60C during lengthy EDTA decalcification, and possible damage to heat labile antigens. There are publications on using EDTA to decalcify fresh bone samples, then snap freeze, cut frozen sections, then acetone fix the sections for murine CD markers compromised by both NBF and acid decalcification. EDTA mixtures are found in Histonet Archives, histotechnology books and on websites e.g. IHCworld, as a chelating agent, it is a very slow decalcifier. More advantages of EDTA are - no damage to antigens, nucleic acid staining, nor other tissue components. It does affect the enzyme histochemical stain for alkaline phosphatase, and magnesium ions (chelated by EDTA) must be replaced in the staining solution. Most clinical laboratories prefer to use formic acid or hydrochloric acid decalcifying solutions instead of EDTA when rapid diagnosis is needed. If you try EDTA alone, rinse the bone well (after decalcification) with running tap water since residual, excess EDTA in tissue precipitates in presence of alcohol, and the ppt makes the tissue difficult to section. Endpoint determinations for complete removal of calcium with EDTA cannot be done chemically, although there is a weight gain/weight loss method that we use routinely that works or use an Xray machine, Faxitron. You can buy EDTA mixtures from Poly Scientific and some other suppliers, or make it up yourself. Otherwise, try the formic acid with EDTA, it may work very well for you while not sacrificing speed for diagnosis. Formic acid will be less damaging to antigens than hydrochloric acid, and is a popular decalcifier because it is gentler than HCl. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 4881 (20100219) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4881 (20100219) __________ The message was checked by ESET Smart Security. http://www.eset.com From jphistology <@t> gmail.com Fri Feb 19 22:46:28 2010 From: jphistology <@t> gmail.com (Joao Pessoa) Date: Fri Feb 19 22:46:34 2010 Subject: [Histonet] Distance learning? In-Reply-To: References: Message-ID: <9e4d12d1002192046h29bc5cfbo48367b74d9a36bf9@mail.gmail.com> You should try Harford Community College. I know several histo techs who have completed histo tech training through the Harford on-line program, and the practical or technical portions in their own lab (employer must approve). http://www.harford.edu/cet/histotech/default.asp Thanks, Joao Histo Tech On Fri, Feb 12, 2010 at 7:25 AM, Jay Lundgren wrote: > A few years ago I remember hearing about a NAACLS approved, distance > learning Histotechnology program. They were using the Internet and > teleconferencing to train students. Is anyone still doing this, and could > you tell me where? > > Thanks for your time, > > Jay A. > Lundgren, M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkiernan <@t> uwo.ca Sat Feb 20 01:40:54 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Feb 20 01:40:59 2010 Subject: [Histonet] Re: DRGs In-Reply-To: References: Message-ID: Good on yer, Bob! There are indeed far too many abbreviations. Anything not in the Abbreviations appendix of an ordinary dictionary (volume = one litre or less, as on the average family's shelf) should be explained. In this case my interpretation was DRG = dorsal root ganglia. Dissecting them out of rats is a difficult job even for the easiest one (C2), and a rat is 10 times as big as a mouse! The only easy-to-remove rat or mouse sensory ganglion is the trigeminal. Cheers, John John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Robert Richmond Date: Friday, February 19, 2010 14:08 Subject: [Histonet] Re: DRGs To: histonet@lists.utsouthwestern.edu > DRG's for mice? Do mice have Medicare now? I hope the pending health > care reform legislation addresses this issue - it's no wonder costs > are spiraling out of sight! > > I have the feeling that a mouse DRG might be something other than > "Diagnosis Related Group", the coding scheme that has determined > Medicare (and many other insurers') payments to hospitals for > close to > thirty years now. > > Histonet includes many people from diverse disciplines. It's better > not to assume that any but the commonest abbreviations (H & E > and not > too many more) will be understood by every Histonetter. > > Bob Richmond > Samurai Pathologist > Knoxville TN (I mean Tennessee) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shehnazster <@t> gmail.com Sat Feb 20 07:54:32 2010 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Sat Feb 20 07:54:40 2010 Subject: [Histonet] shandon's formal fixx and cytoblock kit Message-ID: <4fe9f16e1002200554r1ff45e23q3a1ba567c24cb8e7@mail.gmail.com> Hi Histonetters Could someone kindly share their views on the shandon's formal fixx (concentrate) and cytoblock kit for cell block preparation in cytology? Has it been effective for cellular preservation and cell capture from inadeduate samples? Thanking you in advance. S .Khan Dept of Cytology University of Witwatersrand Johannesburg South Africa From sohail_e <@t> yahoo.com Sat Feb 20 12:14:22 2010 From: sohail_e <@t> yahoo.com (Sohail Ejaz) Date: Sat Feb 20 12:14:26 2010 Subject: [Histonet] Staining Brain Sections with NeuN Message-ID: <320418.93672.qm@web39503.mail.mud.yahoo.com> Dear Friends I am staining brain sections (30 micro meter) with NeuN antibody to evaluate neuronal loss after stroke in Rat. I am using under mentioned protocol which worked fine during first attempt but afterward i am not getting any signal. Please have a look at the protocol and guide me where i am wrong. ? Quench the free floating sections (10% methanol and 10% H2O2 in distilled water) for 5 min. Wash the sections in Trizma Buffer Saline (TBS) (pH7.4)??.3 X 5 min. Block the sections in 3% normal horse serum with TBS containing 0.2% Triton X-100 (TXTBS) for 1 h at room temperature (RT). Incubate the sections overnight at (RT) with primary antibody (NeuN, monoclonal: dilution 1:1000; MAB377, Chemicon International) in TXTBS containing 1% NHS. Wash the sections with TBS ??.3 X 5 min. Incubate the sections for 3 h (RT) with biotinylated anti-mouse IgG (rat- absorbed: dilution 1:200; Vector) secondary antibody in TXTBS with 1% NHS Wash with TBS ??.3 X 5 min. Incubate the sections with a streptavidin-biotinylated horseradish peroxidase complex (VECTASTAIN Elite ABC Kit) for 2 h (RT).Wash the sections in TBS ??.3 X 5 min. Wash the sections with PBS Incubate the sections with DAB Peroxidase substrate kit. Remove excess stain by thorough washing in PBS Mount the sections onto 1% gelatin-coated slides and air-dry overnight (RT) From burch007 <@t> mc.duke.edu Sat Feb 20 17:05:08 2010 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Sat Feb 20 17:05:16 2010 Subject: [Histonet] North Carolina Histology Meeting Message-ID: To members and friends of the NCSHT: &nbs On behalf of the North Carolina Society of Histopathology Technologist Meeting, April 8 th < < justify Th passion for o gain a wealth of knowledge but to take the time to v laboratory products and instrumentation on the market today. hope you make time t for the NCSHT Spring Meeting, April 8 th - forward to seeing you in RTP! Warmest Regards, The Officers of NCSHT < Lisa Gates or Jim &n President: &nb bsp; L p; & [1]lisa.d.gates@gsk.com &nb [2]burch007@mc.duke.edu References 1. file://localhost/tmp/3D"mai 2. ="mailto:burch007@mc.duke.edu" From jkiernan <@t> uwo.ca Sat Feb 20 17:22:44 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Feb 20 17:22:49 2010 Subject: [Histonet] Reticulin stain Gomori Message-ID: This is a difficult one! Ferric ammonium sulphate (iron alum) isn't the only metal salt used as a "sensitizer" between the permanganate oxidation and the ammoniacal silver. Other methods use a uranyl salt or silver nitrate (cf Bielschowsky and its variants), and probably there are others. In some methods this step is omitted. The trouble is that calling the iron alum or other solution a "sensitizer" isn't really saying anything. One could speculate on possible chemical reactions, especially for a ferric salt, but speculations need experimental testing. The two best papers that I know on mechanisms involved in these methods are: Velican C & Velican D 1970. Acta Anat. 77: 540-559 and Puchtler H & Waldrop FS 1978 Histochemistry 57: 177-187. The second of these includes experimental work and extensive literature review and concludes that ". . . silver impregnation technics for reticulum fibers have no chemical significance and cannot be considered as histochemical technics for "reticulin" or type III collagen". Note the different usage of "reticulum fibers" (histology) and "reticulin" (from late 19th century biochemistry). The Velicans provided evidence supporting the detection of aldehydes produced by oxidation of carbohydrate components associated with reticulum fibres and basement membranes. If anyone knows of histochemical investigations in this field more recent than 1978, please let us all know. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Gudrun Lang Date: Thursday, February 18, 2010 9:07 Subject: [Histonet] reticulin stain Gomori To: histonet@lists.utsouthwestern.edu > Hi all! > > Today I was asked if I knew the chemistry behind the ammonia- > iron-sulfat > NH4Fe(SO4)2 . 12H2O reaction in the reticulin stain of Gomori. > > I couldn't help so I forward the question to the histonet comunity. > > I hope, someone has a suggestion for me. > > > > Gudrun Lang > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From o.isaac24 <@t> yahoo.com Sat Feb 20 20:38:41 2010 From: o.isaac24 <@t> yahoo.com (Isaac O) Date: Sat Feb 20 20:38:45 2010 Subject: [Histonet] CRYOSTAT DECONTAMINATION Message-ID: <449670.69198.qm@web111615.mail.gq1.yahoo.com> Hi, ??? I will appreciate it, if you guys could share your procedure for Cryostat Decontamination with me. Thanking you all for your anticipated cooperation. ?Isaac. From raj <@t> bluemarble.net Sat Feb 20 21:13:54 2010 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Sat Feb 20 21:14:00 2010 Subject: [Histonet] Mohs Message-ID: Are any of you using the Sakura Prisma Stainer for H&E and Mohs staining? Thanks raj From pruegg <@t> ihctech.net Sun Feb 21 14:16:18 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Feb 21 14:17:00 2010 Subject: [Histonet] DRGs In-Reply-To: <37E4BAC017F57141AF64FAA5AEB04CE8033A7A0D@wlmmsx01.nemours.org> References: <37E4BAC017F57141AF64FAA5AEB04CE8033A7A0D@wlmmsx01.nemours.org> Message-ID: <5D5525F1820E4623866C27CB41D2DB1F@prueggihctechlt> Carol, I have had problems with cutting samples setup in histogel before but I can't figure out why, the problem is inconsistent, sometimes the samples are fine and other times it acts like the histogel prevented the infiltration of processing reagents into the tissue. I have even cut the histogel away (it has done it's job of keeping the small tissue oriented) and reprocessed the tissue with some good results. Melt the block and cut the histogel away with a razor blade, it just surrounds the tissue and does not really infiltrate it, then gently squeeze the melted paraffin out while on a hot embedding plate between paper towel, no need to remove paraffin with xylene, just re cassette the tissue keeping track of the orientation and put it back thru the processor. Some theories I have for why histogel embedded samples cut well sometimes and not others include: 1. Maybe the histogel got too cold during the setup process, I try to make sure the histogel is completely liquid (warmed) when I put it in the mold with the tissue and then let them cool together, I think sometimes if I am preparing a bunch of samples the last ones start to setup before I put the tissue in the mold with the histogel, but I have not done a scientific study of this. 2. I think that it might be better to go into formalin rather than 70% alcohol after preparing the histogel embedded block as the alcohol may harden the histogel too much too quickly preventing infiltration? Again, just a theory not tested in a controlled way?? Sometimes my samples are already in alcohol and sometimes they are in formalin so I have been putting them back into what they came in. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Thursday, February 18, 2010 11:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DRGs Importance: High Histonetter's...we received a "boat-load" of mouse DRGs that had been prepared in histogel and are cutting...well..not so good. We normally do DRGs from FS and get beautiful results. We have used histogel before with other small sample and have never had issues... not sure if it is the Histogel or DRG's (fixed in 10% NBF and then transferred to 70% before placed into the histogel).is the issue..I seem to remember that histogel requires formalin and wonder if the transfer to 70% is causing our problem ...but, obviously there is not much room for error with such tiny- tiny samples and they are already process and in paraffin? I am not quite sure how twe can improve the ones that came in histogel, and were processed to paraffin a paraffin block....any idea's? any experience? any anything? Thx- ASAP! cbarone@nemours.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathmaster <@t> yahoo.com Sun Feb 21 14:19:33 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Sun Feb 21 14:19:38 2010 Subject: [Histonet] Cryostat decon- what a pain in the butt Message-ID: <501976.56369.qm@web111113.mail.gq1.yahoo.com> CAP is moving to more rigorous cryostat decontamnation methods - mandating a weekly shutdown and wet chemical disinfection with a tuberculocidal agent for machines used regularly. Now, if a lab is doing 1-3 frozens a week, is that used regularly? We must lobby the CAP for more sensible and practical guidlines. The old wipe down with 70% ETOH without bringing the machine will become non-compliant and useful only as an interim measure. ? By the way, the UV lamps do not satisfy the CAP standard, I believe. ? Our system has gone to a commercially available tuberculocidal, virucidal, and broad spectrum bacteriocidal moistened wipes the name of which I will post tomorrow when I get to the job. ? Here is a skeletonized basic procedure form what CAP will require: ? 1: Remove all utensils and brush out and collect section debris disposing of this according to regulated medical waste protocol (red bag). 2. Bring the instrument to room temperature. 3: Wipe all working surfaces with the tuberculocidal wipes, visibly moistening all surfaces. Surface must remain wet for 2 minutes. Use multiple wipes as needed. Instruments can be similarly disinfected. 4: Carefully dry all surfaces with gauze. Dispose of all wipes and gauze as biohazardous. 5: Dry and lubricate the microtome as per manufacturer's instructions. 6. Turn on crystat and bring to working temperature. 7. Document procedure on your maintenance log. 8. Look forward to doing it again next week. ? ? Look, this is a necessary procedure, but weekly??? Perhaps some workload- based formula or an alert system- like pathologists alert the lab when? a case with granulomas or caseating necrosis is sectioned.? Every lab will have to bring a tech in on weekends or at night,?to do this, or have two cryostats to compensate for the fully one working day most machines will have to be down to be cleaned in this manner. ? Thoughts? ? Jeff Silverman ? ? From wingman320 <@t> hotmail.com Sun Feb 21 16:39:12 2010 From: wingman320 <@t> hotmail.com (Harlem Kaputnik) Date: Sun Feb 21 16:39:16 2010 Subject: [Histonet] Tissue repository In-Reply-To: <9e4d12d1002191321o6a98e4f6o481f75b6c603c393@mail.gmail.com> References: <9e4d12d1002191321o6a98e4f6o481f75b6c603c393@mail.gmail.com> Message-ID: Contact Michael Beske, St. Mary's Hospital, Rhinelander, Wisconsin. www.ministryhealth.org, tel: 715-361-2000. Mike has a control block exchange program. He may be able to help you. > Date: Fri, 19 Feb 2010 13:21:09 -0800 > From: jphistology@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Tissue repository > > Dear histonet, > > Does anyone know where I can find a tissue repository to exchange tissue > blocks? For example, I have some extra melanoma FFPE tissue blocks but > would like some normal FFPE tonsil tissue blocks. I do not wish to buy > blocks, but rather trade. Please let me know if there is such a place. > > Thanks, > > Joao > Histo Tech > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/ From wingman320 <@t> hotmail.com Sun Feb 21 16:42:55 2010 From: wingman320 <@t> hotmail.com (Harlem Kaputnik) Date: Sun Feb 21 16:43:03 2010 Subject: [Histonet] Staining Brain Sections with NeuN In-Reply-To: <320418.93672.qm@web39503.mail.mud.yahoo.com> References: <320418.93672.qm@web39503.mail.mud.yahoo.com> Message-ID: What did your control tissue tell you.... as in staining of the positive control, and your negative positive control as well? > Date: Sat, 20 Feb 2010 10:14:22 -0800 > From: sohail_e@yahoo.com > To: histonet@lists.utsouthwestern.edu > CC: histonet-request@lists.utsouthwestern.edu; histonet-owner@lists.utsouthwestern.edu > Subject: [Histonet] Staining Brain Sections with NeuN > > Dear Friends > > I am staining brain sections (30 micro meter) with NeuN antibody to evaluate neuronal loss after stroke in Rat. > > I am using under mentioned protocol which worked fine during first attempt but afterward i am not getting any signal. > > Please have a look at the protocol and guide me where i am wrong. > > > > > > > Quench > the free floating sections (10% methanol and 10% H2O2 in distilled water) > for 5 min. > > > > Wash > the sections in Trizma Buffer Saline (TBS) (pH7.4)??.3 X 5 min. > > > > Block > the sections in 3% normal horse serum with TBS containing 0.2% Triton X-100 > (TXTBS) for 1 h at room temperature (RT). > > > > Incubate > the sections overnight at (RT) with primary antibody (NeuN, monoclonal: > dilution 1:1000; MAB377, Chemicon International) in TXTBS containing 1% > NHS. > > > > Wash > the sections with TBS ??.3 X 5 min. > > > > Incubate > the sections for 3 h (RT) with biotinylated anti-mouse IgG (rat- absorbed: > dilution 1:200; Vector) secondary antibody in TXTBS with 1% NHS > > > > Wash > with TBS ??.3 X 5 min. > > > > Incubate the sections with a > streptavidin-biotinylated horseradish peroxidase complex (VECTASTAIN Elite > ABC Kit) for 2 h (RT).Wash > the sections in TBS ??.3 X 5 min. > > > > Wash > the sections with PBS > > > > Incubate > the sections with DAB Peroxidase substrate kit. > > > > Remove > excess stain by thorough washing in PBS > > > > Mount > the sections onto 1% gelatin-coated slides and air-dry overnight (RT) > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/ From wingman320 <@t> hotmail.com Sun Feb 21 16:48:29 2010 From: wingman320 <@t> hotmail.com (Harlem Kaputnik) Date: Sun Feb 21 16:48:35 2010 Subject: [Histonet] Distance learning? In-Reply-To: <9e4d12d1002192046h29bc5cfbo48367b74d9a36bf9@mail.gmail.com> References: , <9e4d12d1002192046h29bc5cfbo48367b74d9a36bf9@mail.gmail.com> Message-ID: Look into the schooling carefully. The training will help you, but will your employer acknowledge it with compensation?? Today, many employers don't care if they have good quality technicians, they will hire anyone. This way they don't have to pay for someone with knowledge. If you choose to go back to school, choose a profession that will will appreciate your schooling and respect your knowledge accordingly with fair compensation. > Date: Fri, 19 Feb 2010 20:46:28 -0800 > From: jphistology@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Distance learning? > > You should try Harford Community College. I know several histo techs who > have completed histo tech training through the Harford on-line program, and > the practical or technical portions in their own lab (employer must > approve). > > http://www.harford.edu/cet/histotech/default.asp > > Thanks, > > Joao > Histo Tech > > On Fri, Feb 12, 2010 at 7:25 AM, Jay Lundgren wrote: > > > A few years ago I remember hearing about a NAACLS approved, distance > > learning Histotechnology program. They were using the Internet and > > teleconferencing to train students. Is anyone still doing this, and could > > you tell me where? > > > > Thanks for your time, > > > > Jay A. > > Lundgren, M.S., HTL (ASCP) > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/ From cbarone <@t> NEMOURS.ORG Sun Feb 21 19:19:04 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Sun Feb 21 19:19:11 2010 Subject: [Histonet] RE: Histonet Digest, Vol 75, Issue 26 In-Reply-To: References: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7A2E@wlmmsx01.nemours.org> DRG's = Dorsal Root Ganglion... To clarify....healthcare is safe from these. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, February 20, 2010 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IF staining on peritoneal macrophages (Lecorchick, William) 2. EDTA (Webb, Dorothy L) 3. Re: DRGs (Robert Richmond) 4. Re: EDTA (Rene J Buesa) 5. Tissue repository (Joao Pessoa) 6. Re: Tissue repository (Rene J Buesa) 7. seeking histology position (Pathrm35@comcast.net) 8. Re: EDTA (gayle callis) 9. RE: Re: EDTA (Rittman, Barry R) 10. RE: Re: EDTA (gayle callis) 11. Re: Distance learning? (Joao Pessoa) 12. Re: Re: DRGs (John Kiernan) 13. shandon's formal fixx and cytoblock kit (shehnaz khan) ---------------------------------------------------------------------- Message: 1 Date: Fri, 19 Feb 2010 13:17:51 -0500 From: "Lecorchick, William" Subject: [Histonet] IF staining on peritoneal macrophages To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71A4AB78B43@HAMEXMBA.rwjham.local> Content-Type: text/plain; charset="us-ascii" You may want to try using the "NuView" Cyto prep technique from QC sciences it is a good alternative to a Cytospin ------------------------------ Message: 2 Date: Fri, 19 Feb 2010 12:29:01 -0600 From: "Webb, Dorothy L" Subject: [Histonet] EDTA To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C57696D718@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. Thank you ahead of time for your advice! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 3 Date: Fri, 19 Feb 2010 14:07:37 -0500 From: Robert Richmond Subject: [Histonet] Re: DRGs To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 DRG's for mice? Do mice have Medicare now? I hope the pending health care reform legislation addresses this issue - it's no wonder costs are spiraling out of sight! I have the feeling that a mouse DRG might be something other than "Diagnosis Related Group", the coding scheme that has determined Medicare (and many other insurers') payments to hospitals for close to thirty years now. Histonet includes many people from diverse disciplines. It's better not to assume that any but the commonest abbreviations (H & E and not too many more) will be understood by every Histonetter. Bob Richmond Samurai Pathologist Knoxville TN (I mean Tennessee) ------------------------------ Message: 4 Date: Fri, 19 Feb 2010 13:03:27 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] EDTA To: "'histonet@lists.utsouthwestern.edu'" , Dorothy LWebb Message-ID: <796184.91241.qm@web65714.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 EDTA (which stands for Ethylene-diamino-tratacetic-acid) is a chelating agent and the method of choice to decalcify BM biopsies. The thing is that it should be used alone, and not combined with any acid, as you state (not even formic acid). By itself will produce an extremely gentle decalcification that will be completely suitable for IHC studies. Ren? J. --- On Fri, 2/19/10, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] EDTA To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, February 19, 2010, 1:29 PM I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix.? I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. Thank you ahead of time for your advice! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 19 Feb 2010 13:21:09 -0800 From: Joao Pessoa Subject: [Histonet] Tissue repository To: histonet@lists.utsouthwestern.edu Message-ID: <9e4d12d1002191321o6a98e4f6o481f75b6c603c393@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Dear histonet, Does anyone know where I can find a tissue repository to exchange tissue blocks? For example, I have some extra melanoma FFPE tissue blocks but would like some normal FFPE tonsil tissue blocks. I do not wish to buy blocks, but rather trade. Please let me know if there is such a place. Thanks, Joao Histo Tech ------------------------------ Message: 6 Date: Fri, 19 Feb 2010 13:25:07 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Tissue repository To: histonet@lists.utsouthwestern.edu, Joao Pessoa Message-ID: <464358.67154.qm@web65702.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Contact the NSH that I think offers such a service. Ren? J. --- On Fri, 2/19/10, Joao Pessoa wrote: From: Joao Pessoa Subject: [Histonet] Tissue repository To: histonet@lists.utsouthwestern.edu Date: Friday, February 19, 2010, 4:21 PM Dear histonet, Does anyone know where I can find a tissue repository to exchange tissue blocks?? For example, I have some extra melanoma FFPE tissue blocks but would like some normal FFPE tonsil tissue blocks.? I do not wish to buy blocks, but rather trade.? Please let me know if there is such a place. Thanks, Joao Histo Tech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 19 Feb 2010 22:03:50 +0000 (UTC) From: Pathrm35@comcast.net Subject: [Histonet] seeking histology position To: histonet@lists.utsouthwestern.edu Message-ID: <114145463.6366871266617030451.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 Fellow techs, I am currently seeking??a F/T position as a bench tech. I am ASCP certified and Florida licensed w/ a BS degree.??Relocation and temp. positions are a possibility. Please feel free to pass this e mail along if you know of anyone looking for a tech. Thanks in advance Ron Martin ------------------------------ Message: 8 Date: Fri, 19 Feb 2010 15:03:46 -0700 From: "gayle callis" Subject: [Histonet] Re: EDTA To: "'Histonet'" Message-ID: <000101cab1af$698cf8c0$3ca6ea40$@callis@bresnan.net> Content-Type: text/plain; charset="us-ascii" You wrote: I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. **************************************************************************** **************************************************************************** ** The purpose of EDTA in an acid decalcifier is probably NOT performing the actual removal of calcium (or very little) from the bone but chelating calcium ionized from bone by the acid that then (the calcium) settles to the bottom of the container. I doubt very much that you will be dealing with EDTA but with formic acid decalcification. People who have developed acid decalcifying solutions, with either formic acid or hydrochloric acid tout that the bone section looks very good, better that if EDTA is not present in the solution. I never tested this claim in our bone studies, but it might be something to try, an acid solution containing EDTA and one without EDTA (keeping the same kind of acid in the study). The acid is going to ionize the calcium from bone at a faster rate than the EDTA can chelate the calcium. But most importantly, EDTA does not work in a low pH environment. The chemistry of how EDTA acts at different pHs is well documented by chemists, and found in book chapters on EDTA. My physical chemist spouse supplied me with such a chapter. The pH of formic acid is what, pH 3 or so, and if so, EDTA only begins to chelate calcium around pH 4, and when fully protonated at pH 8, decalcifies faster at that pH than at pH 4, or below pH7. The working pH for most EDTA solutions is commonly 7 to 7.6, but when the pH becomes more alkaline ( and 8 is going into alkaline range) then alkaline sensitive protein linkages can be damaged. EDTA, correctly written out by Rene Buesa e.g. ethylene diaminatetraacetiic acid comes in several molecular weights depending on whether is it EDTA without attached sodium, EDTA disodium and EDTA tetrasodium. Tetrasodium is very soluble in water or PBS, but has a very alkaline pH that requires adjustment down to pH 7 to 7.6 or that high pH will damage alkaline sensitive protein linkages. EDTA and disodium EDTA are not as soluble, usually no more than a 10% solution, requiring heat or addition of sodium hydroxide in order to dissolve. EDTA is also expensive, not affected by heat up to 60C but only if the bone is totally fixed with NBF. I would not advise using 60C during lengthy EDTA decalcification, and possible damage to heat labile antigens. There are publications on using EDTA to decalcify fresh bone samples, then snap freeze, cut frozen sections, then acetone fix the sections for murine CD markers compromised by both NBF and acid decalcification. EDTA mixtures are found in Histonet Archives, histotechnology books and on websites e.g. IHCworld, as a chelating agent, it is a very slow decalcifier. More advantages of EDTA are - no damage to antigens, nucleic acid staining, nor other tissue components. It does affect the enzyme histochemical stain for alkaline phosphatase, and magnesium ions (chelated by EDTA) must be replaced in the staining solution. Most clinical laboratories prefer to use formic acid or hydrochloric acid decalcifying solutions instead of EDTA when rapid diagnosis is needed. If you try EDTA alone, rinse the bone well (after decalcification) with running tap water since residual, excess EDTA in tissue precipitates in presence of alcohol, and the ppt makes the tissue difficult to section. Endpoint determinations for complete removal of calcium with EDTA cannot be done chemically, although there is a weight gain/weight loss method that we use routinely that works or use an Xray machine, Faxitron. You can buy EDTA mixtures from Poly Scientific and some other suppliers, or make it up yourself. Otherwise, try the formic acid with EDTA, it may work very well for you while not sacrificing speed for diagnosis. Formic acid will be less damaging to antigens than hydrochloric acid, and is a popular decalcifier because it is gentler than HCl. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ------------------------------ Message: 9 Date: Fri, 19 Feb 2010 16:20:25 -0600 From: "Rittman, Barry R" Subject: RE: [Histonet] Re: EDTA To: 'Histonet' Message-ID: <75A0543E23D3A7458012D9E02EDBEC00098CEE3112@UTHCMS1.uthouston.edu> Content-Type: text/plain; charset="us-ascii" I agree with Gayle and Rene I would not even use a mixture of the two. Formic acid demineralization will work fine, the theory of using EDTA with it makes no sense. EDTA demineralization usually uses the disodium salt. EDTA by itself is not very soluble but its sodium salts are. It can be present as mono-, di-, tri- or tetra-sodium salts depending on the pH. At pH around 11 there are 4 groups that can be replaced with for example calcium. Usually the disodium salt of EDTA is used as the tri and tetrasodium salts are only present in very alkaline solutions that tend to cause tissue maceration. At acidic pH the EDTA will only work slowly and have one group replaceable as the monosodium EDTA. There are several good formulae for using EDTA and formic acid in separate formulae, depending on your specific needs.. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis [gayle.callis@bresnan.net] Sent: Friday, February 19, 2010 4:03 PM To: 'Histonet' Subject: [Histonet] Re: EDTA You wrote: I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. **************************************************************************** **************************************************************************** ** The purpose of EDTA in an acid decalcifier is probably NOT performing the actual removal of calcium (or very little) from the bone but chelating calcium ionized from bone by the acid that then (the calcium) settles to the bottom of the container. I doubt very much that you will be dealing with EDTA but with formic acid decalcification. People who have developed acid decalcifying solutions, with either formic acid or hydrochloric acid tout that the bone section looks very good, better that if EDTA is not present in the solution. I never tested this claim in our bone studies, but it might be something to try, an acid solution containing EDTA and one without EDTA (keeping the same kind of acid in the study). The acid is going to ionize the calcium from bone at a faster rate than the EDTA can chelate the calcium. But most importantly, EDTA does not work in a low pH environment. The chemistry of how EDTA acts at different pHs is well documented by chemists, and found in book chapters on EDTA. My physical chemist spouse supplied me with such a chapter. The pH of formic acid is what, pH 3 or so, and if so, EDTA only begins to chelate calcium around pH 4, and when fully protonated at pH 8, decalcifies faster at that pH than at pH 4, or below pH7. The working pH for most EDTA solutions is commonly 7 to 7.6, but when the pH becomes more alkaline ( and 8 is going into alkaline range) then alkaline sensitive protein linkages can be damaged. EDTA, correctly written out by Rene Buesa e.g. ethylene diaminatetraacetiic acid comes in several molecular weights depending on whether is it EDTA without attached sodium, EDTA disodium and EDTA tetrasodium. Tetrasodium is very soluble in water or PBS, but has a very alkaline pH that requires adjustment down to pH 7 to 7.6 or that high pH will damage alkaline sensitive protein linkages. EDTA and disodium EDTA are not as soluble, usually no more than a 10% solution, requiring heat or addition of sodium hydroxide in order to dissolve. EDTA is also expensive, not affected by heat up to 60C but only if the bone is totally fixed with NBF. I would not advise using 60C during lengthy EDTA decalcification, and possible damage to heat labile antigens. There are publications on using EDTA to decalcify fresh bone samples, then snap freeze, cut frozen sections, then acetone fix the sections for murine CD markers compromised by both NBF and acid decalcification. EDTA mixtures are found in Histonet Archives, histotechnology books and on websites e.g. IHCworld, as a chelating agent, it is a very slow decalcifier. More advantages of EDTA are - no damage to antigens, nucleic acid staining, nor other tissue components. It does affect the enzyme histochemical stain for alkaline phosphatase, and magnesium ions (chelated by EDTA) must be replaced in the staining solution. Most clinical laboratories prefer to use formic acid or hydrochloric acid decalcifying solutions instead of EDTA when rapid diagnosis is needed. If you try EDTA alone, rinse the bone well (after decalcification) with running tap water since residual, excess EDTA in tissue precipitates in presence of alcohol, and the ppt makes the tissue difficult to section. Endpoint determinations for complete removal of calcium with EDTA cannot be done chemically, although there is a weight gain/weight loss method that we use routinely that works or use an Xray machine, Faxitron. You can buy EDTA mixtures from Poly Scientific and some other suppliers, or make it up yourself. Otherwise, try the formic acid with EDTA, it may work very well for you while not sacrificing speed for diagnosis. Formic acid will be less damaging to antigens than hydrochloric acid, and is a popular decalcifier because it is gentler than HCl. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 19 Feb 2010 16:32:13 -0700 From: "gayle callis" Subject: RE: [Histonet] Re: EDTA To: "'Rittman, Barry R'" , "'Histonet'" Message-ID: <000301cab1bb$c4e91210$4ebb3630$@callis@bresnan.net> Content-Type: text/plain; charset="us-ascii" We use EDTA tetra-sodium salt, pH 7.6 but the pH is adjusted down with glacial acetic acid using continuous stirring and a pH electrode in the solution (titration with the acid). This is a recipe from a Dr. Webster (Webb) S. S. Jee publication. We like this since this 14% EDTA dissolves immediately in water or PBS compared disodium EDTA, and at a higher concentration = more molecules of EDTA available. We are very careful to NOT use this 14% tetrasodium EDTA at it original pH of around 10 or more. Decalcification is still very slow, but a recent bone project required EDTA decalcification (so we thought!) to protect antigens never stained before in our lab. After trying both the EDTA and 10% formic acid, we found the antigen to be very robust after formic acid decalcification too. Lesson learned, don't put off a pilot study to test IHC after acid decalcification. The formic acid would have been faster and cheaper. Gayle Callis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Friday, February 19, 2010 3:20 PM To: 'Histonet' Subject: RE: [Histonet] Re: EDTA I agree with Gayle and Rene I would not even use a mixture of the two. Formic acid demineralization will work fine, the theory of using EDTA with it makes no sense. EDTA demineralization usually uses the disodium salt. EDTA by itself is not very soluble but its sodium salts are. It can be present as mono-, di-, tri- or tetra-sodium salts depending on the pH. At pH around 11 there are 4 groups that can be replaced with for example calcium. Usually the disodium salt of EDTA is used as the tri and tetrasodium salts are only present in very alkaline solutions that tend to cause tissue maceration. At acidic pH the EDTA will only work slowly and have one group replaceable as the monosodium EDTA. There are several good formulae for using EDTA and formic acid in separate formulae, depending on your specific needs.. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis [gayle.callis@bresnan.net] Sent: Friday, February 19, 2010 4:03 PM To: 'Histonet' Subject: [Histonet] Re: EDTA You wrote: I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. **************************************************************************** **************************************************************************** ** The purpose of EDTA in an acid decalcifier is probably NOT performing the actual removal of calcium (or very little) from the bone but chelating calcium ionized from bone by the acid that then (the calcium) settles to the bottom of the container. I doubt very much that you will be dealing with EDTA but with formic acid decalcification. People who have developed acid decalcifying solutions, with either formic acid or hydrochloric acid tout that the bone section looks very good, better that if EDTA is not present in the solution. I never tested this claim in our bone studies, but it might be something to try, an acid solution containing EDTA and one without EDTA (keeping the same kind of acid in the study). The acid is going to ionize the calcium from bone at a faster rate than the EDTA can chelate the calcium. But most importantly, EDTA does not work in a low pH environment. The chemistry of how EDTA acts at different pHs is well documented by chemists, and found in book chapters on EDTA. My physical chemist spouse supplied me with such a chapter. The pH of formic acid is what, pH 3 or so, and if so, EDTA only begins to chelate calcium around pH 4, and when fully protonated at pH 8, decalcifies faster at that pH than at pH 4, or below pH7. The working pH for most EDTA solutions is commonly 7 to 7.6, but when the pH becomes more alkaline ( and 8 is going into alkaline range) then alkaline sensitive protein linkages can be damaged. EDTA, correctly written out by Rene Buesa e.g. ethylene diaminatetraacetiic acid comes in several molecular weights depending on whether is it EDTA without attached sodium, EDTA disodium and EDTA tetrasodium. Tetrasodium is very soluble in water or PBS, but has a very alkaline pH that requires adjustment down to pH 7 to 7.6 or that high pH will damage alkaline sensitive protein linkages. EDTA and disodium EDTA are not as soluble, usually no more than a 10% solution, requiring heat or addition of sodium hydroxide in order to dissolve. EDTA is also expensive, not affected by heat up to 60C but only if the bone is totally fixed with NBF. I would not advise using 60C during lengthy EDTA decalcification, and possible damage to heat labile antigens. There are publications on using EDTA to decalcify fresh bone samples, then snap freeze, cut frozen sections, then acetone fix the sections for murine CD markers compromised by both NBF and acid decalcification. EDTA mixtures are found in Histonet Archives, histotechnology books and on websites e.g. IHCworld, as a chelating agent, it is a very slow decalcifier. More advantages of EDTA are - no damage to antigens, nucleic acid staining, nor other tissue components. It does affect the enzyme histochemical stain for alkaline phosphatase, and magnesium ions (chelated by EDTA) must be replaced in the staining solution. Most clinical laboratories prefer to use formic acid or hydrochloric acid decalcifying solutions instead of EDTA when rapid diagnosis is needed. If you try EDTA alone, rinse the bone well (after decalcification) with running tap water since residual, excess EDTA in tissue precipitates in presence of alcohol, and the ppt makes the tissue difficult to section. Endpoint determinations for complete removal of calcium with EDTA cannot be done chemically, although there is a weight gain/weight loss method that we use routinely that works or use an Xray machine, Faxitron. You can buy EDTA mixtures from Poly Scientific and some other suppliers, or make it up yourself. Otherwise, try the formic acid with EDTA, it may work very well for you while not sacrificing speed for diagnosis. Formic acid will be less damaging to antigens than hydrochloric acid, and is a popular decalcifier because it is gentler than HCl. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 4881 (20100219) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4881 (20100219) __________ The message was checked by ESET Smart Security. http://www.eset.com ------------------------------ Message: 11 Date: Fri, 19 Feb 2010 20:46:28 -0800 From: Joao Pessoa Subject: Re: [Histonet] Distance learning? To: histonet@lists.utsouthwestern.edu Message-ID: <9e4d12d1002192046h29bc5cfbo48367b74d9a36bf9@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 You should try Harford Community College. I know several histo techs who have completed histo tech training through the Harford on-line program, and the practical or technical portions in their own lab (employer must approve). http://www.harford.edu/cet/histotech/default.asp Thanks, Joao Histo Tech On Fri, Feb 12, 2010 at 7:25 AM, Jay Lundgren wrote: > A few years ago I remember hearing about a NAACLS approved, distance > learning Histotechnology program. They were using the Internet and > teleconferencing to train students. Is anyone still doing this, and > could you tell me where? > > Thanks for your > time, > > Jay A. > Lundgren, M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Sat, 20 Feb 2010 02:40:54 -0500 From: John Kiernan Subject: Re: [Histonet] Re: DRGs To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; CHARSET=US-ASCII Good on yer, Bob! There are indeed far too many abbreviations. Anything not in the Abbreviations appendix of an ordinary dictionary (volume = one litre or less, as on the average family's shelf) should be explained. In this case my interpretation was DRG = dorsal root ganglia. Dissecting them out of rats is a difficult job even for the easiest one (C2), and a rat is 10 times as big as a mouse! The only easy-to-remove rat or mouse sensory ganglion is the trigeminal. Cheers, John John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Robert Richmond Date: Friday, February 19, 2010 14:08 Subject: [Histonet] Re: DRGs To: histonet@lists.utsouthwestern.edu > DRG's for mice? Do mice have Medicare now? I hope the pending health > care reform legislation addresses this issue - it's no wonder costs > are spiraling out of sight! > > I have the feeling that a mouse DRG might be something other than > "Diagnosis Related Group", the coding scheme that has determined > Medicare (and many other insurers') payments to hospitals for close to > thirty years now. > > Histonet includes many people from diverse disciplines. It's better > not to assume that any but the commonest abbreviations (H & E and not > too many more) will be understood by every Histonetter. > > Bob Richmond > Samurai Pathologist > Knoxville TN (I mean Tennessee) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Sat, 20 Feb 2010 15:54:32 +0200 From: shehnaz khan Subject: [Histonet] shandon's formal fixx and cytoblock kit To: histonet@lists.utsouthwestern.edu Message-ID: <4fe9f16e1002200554r1ff45e23q3a1ba567c24cb8e7@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters Could someone kindly share their views on the shandon's formal fixx (concentrate) and cytoblock kit for cell block preparation in cytology? Has it been effective for cellular preservation and cell capture from inadeduate samples? Thanking you in advance. S .Khan Dept of Cytology University of Witwatersrand Johannesburg South Africa ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 26 **************************************** From pedro.louro <@t> spcorp.com Mon Feb 22 08:15:15 2010 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Mon Feb 22 08:15:24 2010 Subject: [Histonet] Region II Meeting Announcement....Atlantic City Message-ID: SAVE THE DATE! The NJSH is working in partnership with the State Societies of PA, DE, MD, and VA to bring our members the next Region II Meeting. It will be held June 10-12, 2010 at the Clarion Hotel & Convention Center in Atlantic City West, New Jersey. Featured Speaker: Peggy Wenk Director of the HT/HTL Program William Beaumont Hospital in Royal Oak, MI In 2007 Peggy received the NSH Histotechnologist of the Year Award, is currently the Teleconference Coordinator, and over the years has held many other positions supporting NSH. She is an outstanding example of someone committed to the education of histologists nationwide! Peggy will be presenting on Friday and Saturday. Other great topics from basic to advanced including: MOHS Troubleshooting Histology Stains Validation, QC & Troubleshooting IHC Bone Biology and Histomorphometry Prostate Cancer IHC HT Exam Prep LEAN Four Short Seminar Series focused on: VIR, Clinical, Management and Safety Additional topics will be added soon! This year we hope to bring you the biggest vendor exhibit to date. Over 60 vendors were invited to participate. The exhibit will be open Friday at 10AM and will run through Saturday until 4PM. Plan to attend Friday evening's reception with the vendors too. FYI: Rooms will be available at a reduced rate of $89 Single/Double Occupancy for Thursday 6/10 and Friday 6/11. Guests receive complimentary Full American Breakfast, free parking, and free shuttle to the casinos. Look for detailed meeting and registration information in April! Pedro Louro, MBA, QIHC Vice President and Co-Chair Membership Committee New Jersey Society for Histotechnology (NJSH) Ph. 908-473-4501 ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From WesterM <@t> MedImmune.com Mon Feb 22 08:37:21 2010 From: WesterM <@t> MedImmune.com (Wester, Martha) Date: Mon Feb 22 08:37:29 2010 Subject: [Histonet] RE: cryostat decontamination In-Reply-To: References: Message-ID: Hi Issac- Our cryostat decon/defrost procedure goes something like this: Remove glass door to cryochamber by pulling up and gently sliding out of track. Allow microtome and chamber to come to room temp. Wipe down exposed surfaces of cryochamber and microtome with 1% bleach or bleach alternative. Wipe dry with paper towels. Spray exposed surfaces with water and wipe dry. Remove cryostat accessories from cryochamber, spraying each (except the brushes) with 1% bleach, then rinsing under RO water. Wipe accessories dry and place in chemical fume hood. Spray thoroughly with absolute alcohol and allow to air dry completely. Disassemble and remove microtome from cryochamber according to manufacturer's instructions found in the owner's manual. Spray microtome and all disassembled parts with 1% bleach and wipe dry with paper towels. Spray with reverse osmosis water and wipe dry with paper towels. Repeat water spray and wipe dry. Place microtome and parts in chemical fume hood. Spray disassembled parts thoroughly with absolute alcohol and allow to air dry completely. Repeat procedure with cryochamber: wipe down with 1% bleach, wipe dry; spray with reverse osmosis water, wipe dry; spray thoroughly with absolute alcohol and allow to air dry completely. Once both cryochamber and microtome are completely dry, reassemble unit according to manufacturer's instructions. Turn main power switch of cryostat on and allow cryostat to reach optimal temperature before cutting sections. If cryostat indicates it needs further drying, turn unit off, use hair dryer to eliminate any moisture and turn on again. Each tech typically cleans up after their turn cutting, by disposing of any shaving, wiping (assembled) knife assembly specimen head, and the areas that collected shavings with 70%ethanol. The external handle of the door and any external control areas used to operate the microtome (ie any areas that gloved hands may have touched) get wiped with a bleach alternative. This is a procedure that our EHS people feel comfortable with given the nature of samples we handle on a daily basis. (and it also wins us points with the manufacturer's Field tech that does our yearly maintenance :) ) Best of luck! ~~~~~~~~~~~~~~~~~ Martha Wester Pathology/Lab Manager MedImmune, LLC. One MedImmune Way Gaithersburg, MD 20878 ***************************************** Message: 4 Date: Sat, 20 Feb 2010 18:38:41 -0800 (PST) From: Isaac O Subject: [Histonet] CRYOSTAT DECONTAMINATION To: histonet@lists.utsouthwestern.edu Message-ID: <449670.69198.qm@web111615.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi, ??? I will appreciate it, if you guys could share your procedure for Cryostat Decontamination with me. Thanking you all for your anticipated cooperation. ?Isaac. **************************************** From algranth <@t> email.arizona.edu Mon Feb 22 08:40:43 2010 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Mon Feb 22 08:40:50 2010 Subject: [Histonet] DRGs In-Reply-To: <37E4BAC017F57141AF64FAA5AEB04CE8033A7A0D@wlmmsx01.nemours.org> References: <37E4BAC017F57141AF64FAA5AEB04CE8033A7A0D@wlmmsx01.nemours.org> Message-ID: Hi Carol, I have used histogel for these kinds of samples and also other small, thin tissues like insect antennae and insect GI tracts and midguts. Since I get all my projects already fixed in whatever fixative the investigator chooses, rinsed and placed in 70% ETOH the histogel never touches formalin. I don't use formalin on my processor but start in 70%. I've never had a problem with the histogel. We just put the sample in the histogel flat and stand it up (turn 90?) when embedding in paraffin. I use tissue prep for embedding. If you don't want to use histogel you could try to put the drg's on GN Metricel membrane disc filters. We do this with a lot of the samples I receive, actually I have the investigators or their techs do this. The tissue sticks to the membrane and orientation is a dream. The membrane presents no problem when sectioning. You can get it from VWR. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Feb 18, 2010, at 11:23 AM, Barone, Carol wrote: > Histonetter's...we received a "boat-load" of mouse DRGs that had been > prepared in histogel and are cutting...well..not so good. > We normally do DRGs from FS and get beautiful results. > > We have used histogel before with other small sample and have never > had > issues... not sure if it is the Histogel or DRG's (fixed in 10% NBF > and > then transferred to 70% before placed into the histogel).is the > issue..I > seem to remember that histogel requires formalin and wonder if the > transfer to 70% is causing our problem ...but, obviously there is not > much room for error with such tiny- tiny samples and they are already > process and in paraffin? > > I am not quite sure how twe can improve the ones that came in > histogel, > and were processed to paraffin a paraffin block....any idea's? any > experience? any anything? Thx- ASAP! > > cbarone@nemours.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken <@t> ucsfmedctr.org Mon Feb 22 10:23:17 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Mon Feb 22 10:23:33 2010 Subject: [Histonet] Cryostat decon- what a pain in the butt In-Reply-To: <501976.56369.qm@web111113.mail.gq1.yahoo.com> References: <501976.56369.qm@web111113.mail.gq1.yahoo.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C0121FF535D@EXMBMCB15.ucsfmedicalcenter.org> Jeff, Do they give any references for the effectiveness of their proposed method? Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Sunday, February 21, 2010 12:20 PM To: o.isaac24@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat decon- what a pain in the butt CAP is moving to more rigorous cryostat decontamnation methods - mandating a weekly shutdown and wet chemical disinfection with a tuberculocidal agent for machines used regularly. Now, if a lab is doing 1-3 frozens a week, is that used regularly? We must lobby the CAP for more sensible and practical guidlines. The old wipe down with 70% ETOH without bringing the machine will become non-compliant and useful only as an interim measure. ? By the way, the UV lamps do not satisfy the CAP standard, I believe. ? Our system has gone to a commercially available tuberculocidal, virucidal, and broad spectrum bacteriocidal moistened wipes the name of which I will post tomorrow when I get to the job. ? Here is a skeletonized basic procedure form what CAP will require: ? 1: Remove all utensils and brush out and collect section debris disposing of this according to regulated medical waste protocol (red bag). 2. Bring the instrument to room temperature. 3: Wipe all working surfaces with the tuberculocidal wipes, visibly moistening all surfaces. Surface must remain wet for 2 minutes. Use multiple wipes as needed. Instruments can be similarly disinfected. 4: Carefully dry all surfaces with gauze. Dispose of all wipes and gauze as biohazardous. 5: Dry and lubricate the microtome as per manufacturer's instructions. 6. Turn on crystat and bring to working temperature. 7. Document procedure on your maintenance log. 8. Look forward to doing it again next week. ? ? Look, this is a necessary procedure, but weekly??? Perhaps some workload- based formula or an alert system- like pathologists alert the lab when? a case with granulomas or caseating necrosis is sectioned.? Every lab will have to bring a tech in on weekends or at night,?to do this, or have two cryostats to compensate for the fully one working day most machines will have to be down to be cleaned in this manner. ? Thoughts? ? Jeff Silverman ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Mon Feb 22 10:32:02 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Feb 22 10:32:05 2010 Subject: [Histonet] Cryostat decon- what a pain in the butt In-Reply-To: <1AAF670737F193429070841C6B2ADD4C0121FF535D@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <1140521359.79991266856322045.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Jeff, Can you give the reference for the CAP "adjustment" on decon for crystats please?? We have two working cryostats and average 6 to 10 cases (not specimens) a day.? This would effectively shut us down for frozens on some days.? We have third one that is currently in for repair. Thanks, Pam Marcum UAMS Anatomic Patholgoy Manager ----- Original Message ----- From: "Tim Morken" To: "Jeffrey Silverman" , "o isaac24" Cc: histonet@lists.utsouthwestern.edu Sent: Monday, February 22, 2010 10:23:17 AM GMT -06:00 US/Canada Central Subject: RE: [Histonet] Cryostat decon- what a pain in the butt Jeff, Do they give any references for the effectiveness of their proposed method? Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Sunday, February 21, 2010 12:20 PM To: o.isaac24@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat decon- what a pain in the butt CAP is moving to more rigorous cryostat decontamnation methods - mandating a weekly shutdown and wet chemical disinfection with a tuberculocidal agent for machines used regularly. Now, if a lab is doing 1-3 frozens a week, is that used regularly? We must lobby the CAP for more sensible and practical guidlines. The old wipe down with 70% ETOH without bringing the machine will become non-compliant and useful only as an interim measure. ? By the way, the UV lamps do not satisfy the CAP standard, I believe. ? Our system has gone to a commercially available tuberculocidal, virucidal, and broad spectrum bacteriocidal moistened wipes the name of which I will post tomorrow when I get to the job. ? Here is a skeletonized basic procedure form what CAP will require: ? 1: Remove all utensils and brush out and collect section debris disposing of this according to regulated medical waste protocol (red bag). 2. Bring the instrument to room temperature. 3: Wipe all working surfaces with the tuberculocidal wipes, visibly moistening all surfaces. Surface must remain wet for 2 minutes. Use multiple wipes as needed. Instruments can be similarly disinfected. 4: Carefully dry all surfaces with gauze. Dispose of all wipes and gauze as biohazardous. 5: Dry and lubricate the microtome as per manufacturer's instructions. 6. Turn on crystat and bring to working temperature. 7. Document procedure on your maintenance log. 8. Look forward to doing it again next week. ? ? Look, this is a necessary procedure, but weekly??? Perhaps some workload- based formula or an alert system- like pathologists alert the lab when? a case with granulomas or caseating necrosis is sectioned.? Every lab will have to bring a tech in on weekends or at night,?to do this, or have two cryostats to compensate for the fully one working day most machines will have to be down to be cleaned in this manner. ? Thoughts? ? Jeff Silverman ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Mon Feb 22 11:01:22 2010 From: portera <@t> msu.edu (Amy Porter) Date: Mon Feb 22 11:01:29 2010 Subject: [Histonet] DRGs In-Reply-To: References: <37E4BAC017F57141AF64FAA5AEB04CE8033A7A0D@wlmmsx01.nemours.org> Message-ID: <001901cab3e0$a9301fb0$fb905f10$@edu> I think it is strange that we are all doing similar techniques and wind up with different outcomes using the histogel. I would be curious how many of us are using the equipment sold with the histogel for warming and cooling opposed to any of us who don't. we did not purchase the equipment and I wonder sometimes if warming the histogel using other means causes some type of breakdown / and do any of you repeatedly reheat the same tube once it has been warmed and resolidified?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Monday, February 22, 2010 9:41 AM To: HISTONET Subject: Re: [Histonet] DRGs Importance: High Hi Carol, I have used histogel for these kinds of samples and also other small, thin tissues like insect antennae and insect GI tracts and midguts. Since I get all my projects already fixed in whatever fixative the investigator chooses, rinsed and placed in 70% ETOH the histogel never touches formalin. I don't use formalin on my processor but start in 70%. I've never had a problem with the histogel. We just put the sample in the histogel flat and stand it up (turn 90?) when embedding in paraffin. I use tissue prep for embedding. If you don't want to use histogel you could try to put the drg's on GN Metricel membrane disc filters. We do this with a lot of the samples I receive, actually I have the investigators or their techs do this. The tissue sticks to the membrane and orientation is a dream. The membrane presents no problem when sectioning. You can get it from VWR. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Feb 18, 2010, at 11:23 AM, Barone, Carol wrote: > Histonetter's...we received a "boat-load" of mouse DRGs that had been > prepared in histogel and are cutting...well..not so good. > We normally do DRGs from FS and get beautiful results. > > We have used histogel before with other small sample and have never > had > issues... not sure if it is the Histogel or DRG's (fixed in 10% NBF > and > then transferred to 70% before placed into the histogel).is the > issue..I > seem to remember that histogel requires formalin and wonder if the > transfer to 70% is causing our problem ...but, obviously there is not > much room for error with such tiny- tiny samples and they are already > process and in paraffin? > > I am not quite sure how twe can improve the ones that came in > histogel, > and were processed to paraffin a paraffin block....any idea's? any > experience? any anything? Thx- ASAP! > > cbarone@nemours.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dunatrsd <@t> sbcglobal.net Mon Feb 22 11:35:10 2010 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Mon Feb 22 11:35:16 2010 Subject: [Histonet] DRGs In-Reply-To: <001901cab3e0$a9301fb0$fb905f10$@edu> References: <37E4BAC017F57141AF64FAA5AEB04CE8033A7A0D@wlmmsx01.nemours.org> <001901cab3e0$a9301fb0$fb905f10$@edu> Message-ID: <946040.14027.qm@web83916.mail.sp1.yahoo.com> I also think that it is strange of the way Histogel processes. I have posted on the Histonet previously about this exact problem. I worked with Jennifer Hofecker when she was at Vanderbilt U.(sent her my Histogel and she sent me hers) and ended up with perfectly processed Histogel blocks at our facility and hers. I processed a couple of blocks last week and they were just terrible. No change in the processing schedule, or the way Histogel was liquefied (placed in hot water that was heated in the microwave). Prior to the last two blocks, I must have processed at least a dozen blocks without any problems. There was an incident where?I placed two histogels in the same cassete. One processed beautifuly and the other was all shrunken and dried up. I do not liquefy the entire tube, rather I scoop out the approximate amount that I need and transfer to another tube to heat up. If there is anyone out there in Histoland that has not had any issues with the Histogel, can you please post your procedure on liquefying the Histogel, method of cooling/solidifying and processing schedule? The only thing that I do that is not exact is I do not know the temp of my hot water when?i place the Histogel to liquefy. I basically have to wait several minutes for the gel to melt and?I use it immediately. Any new information or solution from anyone, would be greatly appreciated. Thank you Dusko Trajkovic ________________________________ From: Amy Porter To: Andrea Grantham ; HISTONET Sent: Mon, February 22, 2010 9:01:22 AM Subject: RE: [Histonet] DRGs I think it is strange that we are all doing similar techniques and wind up with different outcomes using the histogel.? I would be curious how many of us are using the equipment sold with the histogel for warming and cooling opposed to any of us who don't.? we did not purchase the equipment and I wonder sometimes if warming the histogel using other means causes some type of breakdown / and do any of you repeatedly reheat the same tube once it has been warmed and resolidified?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Monday, February 22, 2010 9:41 AM To: HISTONET Subject: Re: [Histonet] DRGs Importance: High Hi Carol, I have used histogel for these kinds of samples and also other small,? thin tissues like insect antennae and insect GI tracts and midguts.? Since I get all my projects already fixed in whatever fixative the? investigator chooses, rinsed and placed in 70% ETOH the histogel never? touches formalin. I don't use formalin on my processor but start in? 70%. I've never had a problem with the histogel. We just put the? sample in the histogel flat and stand it up (turn 90?) when embedding? in paraffin. I use tissue prep for embedding. If you don't want to use histogel you could try to put the drg's on GN? Metricel membrane disc filters. We do this with a lot of the samples I? receive, actually I have the investigators or their techs do this. The? tissue sticks to the membrane and orientation is a dream. The membrane? presents no problem when sectioning. You can get it from VWR. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415? ? Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" -? Paula Sicurello P Please consider the environment before printing this email. On Feb 18, 2010, at 11:23 AM, Barone, Carol wrote: > Histonetter's...we received a "boat-load" of mouse DRGs that had been > prepared in histogel and are cutting...well..not so good. > We normally do DRGs from FS and get beautiful results. > > We have used histogel before with other small sample and have never? > had > issues...? not sure if it is the Histogel or DRG's (fixed in 10% NBF? > and > then transferred to 70% before placed into the histogel).is the? > issue..I > seem to remember that histogel requires formalin and wonder if the > transfer to 70% is causing our problem ...but, obviously there is not > much room for error with such tiny- tiny samples and they are already > process and in paraffin? > > I am not quite sure how twe can improve the ones that came in? > histogel, > and were processed to paraffin a paraffin block....any idea's? any > experience? any anything? Thx- ASAP! > > cbarone@nemours.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWilkinson <@t> adclinic.com Mon Feb 22 11:55:05 2010 From: JWilkinson <@t> adclinic.com (Wilkinson, Joyce E) Date: Mon Feb 22 11:55:14 2010 Subject: [Histonet] Fw: Nail softner Message-ID: Hi Rene, Could you please fax me your procedure for softening nails, you said it's the best and we do a lot of nails. Thanks in advance. Joyce Wilkinson Fax # 512.9013938 From rsrichmond <@t> gmail.com Mon Feb 22 11:57:22 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Feb 22 11:57:25 2010 Subject: [Histonet] Re: cryostat decontamination Message-ID: Jeff Silverman notes that >>CAP is moving to more rigorous cryostat decontamination methods - mandating a weekly shutdown and wet chemical disinfection with a tuberculocidal agent for machines used regularly.<< Cryostats will have to be brought up to room temperature for this procedure. This weekly decontamination routine would take an extraordinary amount of time. In many labs the pathologist would probably be called on to do it, and would probably refuse. I think that a lot of labs will drop CAP accreditation over this issue, unless JCAHO mandates the same routine. I don't know what I would consider an appropriate routine here. It's been a good many years since I cut a frozen section and found granulomatous inflammation suspicious for tuberculosis. (Though I had a paraffin-section case like that last week - don't have the special stain reports yet.) I clearly remember - when I was a resident in one of America's most tuberculous cities forty years ago - a right colon resection for what turned out on frozen section to be not cancer, but tuberculosis involving the lymph nodes around the cecum. I promptly downed the cryostat (no problem, since we had four of them) and had it up to room temperature and was wiping away when the chief (Bill Shelley, of blest memory) came by and observed "Boy, do you still believe in the germ theory of disease?" Bob Richmond Samurai Pathologist Knoxville TN From Albert.Santiago <@t> uphs.upenn.edu Mon Feb 22 12:08:32 2010 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Mon Feb 22 12:08:42 2010 Subject: [Histonet] Nail Softeners Message-ID: Hello Rene, we also do a lot of nails. I was hoping could fax me a copy of your protocol as well. Thank you .....215-662-6539 -Fax Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6008/6539-office 215-662-6150-fax The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From hermeneus21 <@t> gmail.com Mon Feb 22 12:40:39 2010 From: hermeneus21 <@t> gmail.com (Wisam Barkho) Date: Mon Feb 22 12:41:03 2010 Subject: [Histonet] tumor bearing perfusion Message-ID: <152539f21002221040v67277242k3fea41b7dda7ecae@mail.gmail.com> Hello, We are working on an oncology project that requires the administration of fluorescent dyes in tumor bearing mice. The dyes are allowed to circulate and then tumors are collected, homogenized and centrifuged for fluorescent reading. Specifically, we are looking at the dyes in the interstitial fluid and we cannot have any contamination from blood. We are getting a lot of background fluorescence and we think it may be coming from blood in the tissue. We want to perfuse the animal before tumor collection but we think that if we perfusing with saline will affect the interstitial fluid as well. Has anyone tried to perfuse with something that has high molecular weight? Any advice, tips on this? From leiker <@t> buffalo.edu Mon Feb 22 12:55:31 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Feb 22 12:56:19 2010 Subject: [Histonet] tumor bearing perfusion In-Reply-To: <152539f21002221040v67277242k3fea41b7dda7ecae@mail.gmail.com> References: <152539f21002221040v67277242k3fea41b7dda7ecae@mail.gmail.com> Message-ID: <24DEF85587447759FF9933F9@CDYwxp1931.ad.med.buffalo.edu> This sounds a lot like what I did for my master's project. What I believe I did to correct for the autofluorescence from RBCs was to have a group of control tumor-bearing mice perfused with saline only, take readings as you would with the dye samples, and in the analysis subtract out the saline readings as background. In theory you should have a similar amount of blood in your saline samples as in your dye samples, if your groups are large enough to account for mouse-to-mouse (or tumor-to-tumor) variability (which I found tended to be rather large...). Regards, Merced Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. --On Monday, February 22, 2010 10:40 AM -0800 Wisam Barkho wrote: > Hello, > > We are working on an oncology project that requires the administration of > fluorescent dyes in tumor bearing mice. The dyes are allowed to circulate > and then tumors are collected, homogenized and centrifuged for fluorescent > reading. Specifically, we are looking at the dyes in the interstitial > fluid and we cannot have any contamination from blood. We are getting a > lot of background fluorescence and we think it may be coming from blood > in the tissue. We want to perfuse the animal before tumor collection but > we think that if we perfusing with saline will affect the interstitial > fluid as well. Has anyone tried to perfuse with something that has high > molecular weight? Any advice, tips on this? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From andreahooper <@t> rocketmail.com Mon Feb 22 13:03:10 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Mon Feb 22 13:03:22 2010 Subject: [Histonet] Re: EDTA In-Reply-To: <000301cab1bb$c4e91210$4ebb3630$@callis@bresnan.net> Message-ID: <458208.28260.qm@web113109.mail.gq1.yahoo.com> For many years doing IHC on mouse bones,?I have?used 10% EDTA, just pHed up?to where it goes into solution, with great results for frozen section immunofluorescence. It takes 5 days. For FFPE, we use Richard Allan Decal solution which is HCl and EDTA. Also works great and only?takes 1 hour for murine bones. I don't use the acid decal on frozen sections as I find the autofluorescence is too much and I have better antigen preservation with the EDTA for IF. ? Andrea Hooper ? --- On Fri, 2/19/10, gayle callis wrote: From: gayle callis Subject: RE: [Histonet] Re: EDTA To: "'Rittman, Barry R'" , "'Histonet'" Date: Friday, February 19, 2010, 11:32 PM We use EDTA tetra-sodium salt, pH 7.6 but the pH is adjusted down with glacial acetic acid using continuous stirring and a pH electrode in the solution (titration with the acid). This is a recipe from a Dr. Webster (Webb) S. S. Jee publication. We like this since this 14% EDTA dissolves immediately in water or PBS compared disodium EDTA, and at a higher concentration = more molecules of EDTA available.? We are very careful to NOT use this 14% tetrasodium EDTA at it original pH of around 10 or more.? Decalcification is still very slow, but a recent bone project required EDTA decalcification (so we thought!) to protect antigens never stained before in our lab.? After trying both the EDTA and 10% formic acid, we found the antigen to be very robust after formic acid decalcification too. Lesson learned, don't put off a pilot study to test IHC after acid decalcification. The formic acid would have been faster and cheaper.? ? Gayle Callis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Friday, February 19, 2010 3:20 PM To: 'Histonet' Subject: RE: [Histonet] Re: EDTA I agree with Gayle and Rene I would not even use a mixture of the two. Formic acid demineralization will work fine, the theory of using EDTA with it makes no sense. EDTA demineralization usually uses the disodium salt. EDTA by itself is not very soluble but its sodium salts are. It can be present as mono-, di-, tri- or tetra-sodium salts depending on the pH. At pH around 11 there are 4 groups that can be replaced with for example calcium. Usually the disodium salt of EDTA is used as the tri and tetrasodium salts are only present in very alkaline solutions that tend to cause tissue maceration. At acidic pH the EDTA will only work slowly and have one group replaceable as the monosodium EDTA. There are several good formulae for using EDTA and formic acid in separate formulae, depending on your specific needs.. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis [gayle.callis@bresnan.net] Sent: Friday, February 19, 2010 4:03 PM To: 'Histonet' Subject: [Histonet] Re: EDTA You wrote: I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix.? I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. **************************************************************************** **************************************************************************** ** The purpose of EDTA in an acid decalcifier is probably NOT performing the actual removal of calcium (or very little)? from the bone but chelating calcium ionized from bone by the acid that then (the calcium) settles to the bottom of the container.? I doubt very much that you will be dealing with EDTA but with formic acid decalcification.???People who have developed acid decalcifying solutions, with either formic acid or hydrochloric acid tout that the bone section looks very good, better that if EDTA is not present in the solution.? I never tested this claim in our bone studies, but it might be something to try, an acid solution containing EDTA and one without EDTA (keeping the same kind of acid in the study). The acid is going to ionize the calcium from bone at a faster rate than the EDTA can chelate the calcium.? But most importantly, EDTA does not work in a low pH environment.? The chemistry of how EDTA acts at different pHs is well documented by chemists, and found in book chapters on EDTA.? My physical chemist spouse supplied me with such a chapter.? The pH of formic acid is what, pH 3 or so, and if so, EDTA only begins to chelate calcium around pH 4, and when fully protonated at pH 8, decalcifies faster at that pH than at pH 4, or below pH7.? The working pH for most EDTA solutions is commonly 7 to 7.6, but when the pH becomes more alkaline ( and 8 is going into alkaline range)? then alkaline sensitive protein linkages can be damaged. EDTA, correctly written out by Rene Buesa e.g. ethylene diaminatetraacetiic acid comes in several molecular weights depending on whether is it EDTA without attached sodium, EDTA disodium and EDTA tetrasodium.? Tetrasodium is very soluble in water or PBS, but has a very alkaline pH that requires adjustment down to pH 7 to 7.6 or that high pH will damage alkaline sensitive protein linkages.? EDTA and disodium EDTA are not as soluble, usually no more than a 10% solution, requiring heat or addition of sodium hydroxide in order to dissolve.? EDTA is also expensive,? not affected by heat up to 60C but only if the bone is totally fixed with NBF.? I would not advise using 60C during lengthy EDTA decalcification, and possible damage to heat labile antigens.? There are publications on using EDTA to decalcify fresh bone samples, then snap freeze, cut frozen sections, then acetone fix the sections for murine CD markers compromised by both NBF and acid decalcification. EDTA mixtures are found in Histonet Archives,? histotechnology books and on websites e.g. IHCworld, as a chelating agent, it is a very slow decalcifier. More advantages of EDTA are - no damage to antigens, nucleic acid staining, nor other tissue components.? It does affect the enzyme histochemical stain for alkaline phosphatase, and magnesium ions (chelated by EDTA) must be replaced in the staining solution.? Most clinical laboratories prefer to use formic acid or hydrochloric acid decalcifying solutions instead of EDTA when rapid diagnosis is needed. If you try EDTA alone, rinse the bone well (after decalcification) with running tap water since residual, excess EDTA in tissue precipitates in presence of alcohol, and the ppt makes the tissue difficult to section. Endpoint determinations for complete removal of calcium with EDTA cannot be done chemically, although there is a weight gain/weight loss method that we use routinely that works or use an Xray machine, Faxitron.? You can buy EDTA mixtures from Poly Scientific and some other suppliers, or make it up yourself. Otherwise, try the formic acid with EDTA, it may work very well for you while not sacrificing speed for diagnosis. Formic acid will be less damaging to antigens than hydrochloric acid, and is a popular decalcifier because it is gentler than HCl. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 4881 (20100219) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4881 (20100219) __________ The message was checked by ESET Smart Security. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Mon Feb 22 13:07:43 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Mon Feb 22 13:07:48 2010 Subject: [Histonet] IF staining on peritoneal macrophages In-Reply-To: <1b2831cd1002181500r2b6e1066s51c002b619c6fa28@mail.gmail.com> Message-ID: <976333.22064.qm@web113112.mail.gq1.yahoo.com> After cytospinning I always dry my slides thoroughly before moving on to a IHC or IF step. I also use Superfrost Plus slides or silane coated slides to increase adherance. I would do that then try various fixatives. ? You can make a cell pellet as you suggest. Decide ahead of time if you want to fix before embedding (ie: is your antigen preserved in PFA). If so, fix in suspension then spin and resuspend in a small volume of 2% agarose (or Histogel). Then you can embed in OCT for frozen sectioning or wax for FFPE. ? - Andrea Hooper --- On Thu, 2/18/10, Mauricio Avigdor wrote: From: Mauricio Avigdor Subject: [Histonet] IF staining on peritoneal macrophages To: histonet@lists.utsouthwestern.edu Date: Thursday, February 18, 2010, 11:00 PM Greetings all, I am trying to do immunofluorescence on peritoneal macrophages. I am having a couple of issues that I was hoping one of you could help me resolve. Firstly, I am having uneven results with the Cytospin. Cells tend to get washed off the slides during rinses. Does anyone have tips on how to make cells stick a little better to Cytoslides? Secondly, I have not yet found a satisfactory method for fixation. In order to prevent loss of cells when dipping the slides into fixatives, I am having to air dry the slides before I can do anything to them. I tried the Shandon Collection Fluid (ethanol, isopropanol, carbowax) with great success, but it killed the fluorescence. I am leaning towards spinning the lavage fluid until I get a pellet and then resuspending in PBS with a little BSA added. I hope this makes cells stick well enough that I can put the slides in formalin or acetone. Any thoughts are appreciated! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Mon Feb 22 13:37:58 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Feb 22 13:38:03 2010 Subject: [Histonet] frozen sections of cartilage Message-ID: <215543.49688.qm@web50302.mail.re2.yahoo.com> Hi Everyone, Any tips for keeping frozen sections of cartilage from falling off the slides?during?IHC staining?? We should not have to do HIER, but they still fall of the slides quite easily. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From gvdobbin <@t> ihis.org Mon Feb 22 14:12:53 2010 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Feb 22 14:13:01 2010 Subject: [Histonet] glycogen degeneration??? Message-ID: Hi folks, Has anyone experienced glycogen disappearing from previously "excellent" control blocks of liver. A glycogen-laden liver should be consistent throughout should it not? (ie we can't cut through it can we??). Or is oxidation a possible culprit here (not heard of it)? Really interested to get some feedback on this one. Thanks so much, Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From azdudley <@t> hotmail.com Mon Feb 22 14:21:45 2010 From: azdudley <@t> hotmail.com (anita dudley) Date: Mon Feb 22 14:21:50 2010 Subject: [Histonet] cryostat decontamination Message-ID: thank you all for posting about the cryostat decontamination, I have not revised my procedure and we are due for an inspection . I think this is just over kill, our pathologist says we have to do it if cap says. (they also made me throw away all of the dry drys that they said were outdated? now I think they have changed that, I thought that was crazy also.) the way we do it here is if the path thinks it should be down they will tell us. they try to just do smears if it is a know infectious case. I have another question for those who use ventana's benchmark, or I guess anybody that is doing imunos. are you using a universal negative control? or positive and negative with most every case? thanks for your input. everyone have a good day. anita dudley providence hosp mobile alabama _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/ From NMargaryan <@t> childrensmemorial.org Mon Feb 22 14:47:04 2010 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Feb 22 14:49:24 2010 Subject: [Histonet] Bleach in autostainer cost problems? Message-ID: Hi Histonetters, Have you ever had the problem I am having now: light or totally negative staining after service and cleaning the machine with bleach? To explain it better: I have immuno-autostainer from TermoFisher that was working well before I had service from the company. After a representative from the company serviced the machine and cleaned it with bleach, my staining starts to become very light or totally negative on some slides which were running all together on the same day. Unfortunately, I did not realize it immediately and only after several stainings when I expect to see strong staining instead of light like 1+. First of all, I thought it's one of the reagents or my antibody. Then, I checked on the positive slides and finally I came to decision that this is a bleach that was used to clean machine. I can't get in touch with rep almost a week. If you are familiar with this kind of problem, please give me any suggestion. Naira From rjbuesa <@t> yahoo.com Mon Feb 22 14:51:38 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 22 14:51:41 2010 Subject: [Histonet] frozen sections of cartilage In-Reply-To: <215543.49688.qm@web50302.mail.re2.yahoo.com> Message-ID: <264755.21242.qm@web65705.mail.ac4.yahoo.com> You should aim at obtaining?4 objectives: 1- extremely thin sections (as thin as you are able to produce); 2- absolutely wrinkle free; 3- using positivey charged slides, and 4- let the sections air dry completely before starting the IHC Ren? J. --- On Mon, 2/22/10, Kim Merriam wrote: From: Kim Merriam Subject: [Histonet] frozen sections of cartilage To: "Histonet" Date: Monday, February 22, 2010, 2:37 PM Hi Everyone, Any tips for keeping frozen sections of cartilage from falling off the slides?during?IHC staining?? We should not have to do HIER, but they still fall of the slides quite easily. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Feb 22 14:53:28 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 22 14:53:33 2010 Subject: [Histonet] glycogen degeneration??? In-Reply-To: Message-ID: <216121.47818.qm@web65713.mail.ac4.yahoo.com> Air oxidation is the most likely culprit. Will specially show in sections kept at room temperature for more than 3 weeks (as for epitope signal). Ren? J. --- On Mon, 2/22/10, Greg Dobbin wrote: From: Greg Dobbin Subject: [Histonet] glycogen degeneration??? To: Histonet@lists.utsouthwestern.edu Date: Monday, February 22, 2010, 3:12 PM Hi folks, Has anyone experienced glycogen disappearing from previously "excellent" control blocks of liver. A glycogen-laden liver should be consistent throughout should it not? (ie we can't cut through it can we??). Or is oxidation a possible culprit here (not heard of it)? Really interested to get some feedback on this one. Thanks so much, Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE? ? C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Feb 22 14:56:57 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 22 14:57:02 2010 Subject: [Histonet] Bleach in autostainer cost problems? In-Reply-To: Message-ID: <400535.4501.qm@web65704.mail.ac4.yahoo.com> Bleach is the harshest enemy of any biological reaction and should be avoided as much as possible or absolutely eliminated after is used, otherwise can ruin the best staining protocol. Ren? J. --- On Mon, 2/22/10, Margaryan, Naira wrote: From: Margaryan, Naira Subject: [Histonet] Bleach in autostainer cost problems? To: "histonet@lists.utsouthwestern.edu" Date: Monday, February 22, 2010, 3:47 PM Hi Histonetters, Have you ever had the problem I am having now: light or totally negative staining after service and cleaning the machine with bleach? To explain it better: I have immuno-autostainer from TermoFisher that was working well before I had service from the company. After a representative from the company serviced the machine and cleaned it with bleach, my staining starts to become very light or totally negative on some slides which were running all together on the same day. Unfortunately, I did not realize it immediately and only after several stainings when I expect to see strong staining instead of light like 1+. First of all, I thought it's one of the reagents or my antibody. Then, I checked on the positive slides and finally I came to decision that this is a bleach that was used to clean machine. I can't get in touch with rep almost a week. If you are familiar with this kind of problem, please give me any suggestion. Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jenna.nesler <@t> yahoo.com Mon Feb 22 14:57:18 2010 From: jenna.nesler <@t> yahoo.com (Jennifer Nesler) Date: Mon Feb 22 14:57:22 2010 Subject: [Histonet] histo supervisor Message-ID: <675379.10685.qm@web111603.mail.gq1.yahoo.com> Hello! Does anyone know who the current histology lab supervisor is at Central DuPage Hospital in IL? (the person in charge of hiring?) Thanks! From mcauliff <@t> umdnj.edu Mon Feb 22 15:03:02 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Feb 22 15:00:52 2010 Subject: [Histonet] glycogen degeneration??? In-Reply-To: References: Message-ID: <4B82F106.5070807@umdnj.edu> In addition to Rene's comments, fixation may be an issue. Deeper parts of the block may not be as well fixed as the more superficial parts so the farther into the block you go ....... :-( Geoff Greg Dobbin wrote: > Hi folks, > Has anyone experienced glycogen disappearing from previously > "excellent" control blocks of liver. A glycogen-laden liver should be > consistent throughout should it not? (ie we can't cut through it can > we??). Or is oxidation a possible culprit here (not heard of it)? Really > interested to get some feedback on this one. > Thanks so much, > Greg > > Greg Dobbin, R.T. > Chief Technologist, Anatomic Pathology > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > "I find that the harder I work, the > more luck I seem to have." > - Thomas Jefferson > > > ------------------------- > Statement of Confidentiality > This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Margaret.Perry <@t> sdstate.edu Mon Feb 22 15:08:31 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Feb 22 15:08:38 2010 Subject: [Histonet] looking for mouse antibodies Message-ID: Are there good mouse antibodies that will work on dog and cat for the following: Factor 8 GFAP Mycobacterium sp S100a From andreahooper <@t> rocketmail.com Mon Feb 22 15:17:52 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Mon Feb 22 15:18:01 2010 Subject: [Histonet] IHC on N-cadherin, CD146, CD144,ELF97 TRAcP. In-Reply-To: Message-ID: <652088.63114.qm@web113101.mail.gq1.yahoo.com> Hi Julia, ? I am sorry it's taken me months to reply to this email and hopefully it's not too late. I am only catching up on Histonet messages recently. ? I stain for VE-cadherin, CD144 in murine bone routinely. R&D Systems goat anti-mouse VE-cadherin works very well in paraffin and frozen murine bone. ? If doing paraffin, I remove femurs/tibias from mice and fix in 4% PFA overnight at 4 deg C (can also fix 2-4h at RT). I decal in Richard Allan decal solution for 1h, sometimes 1.5h if femurs are especially large and remain hard after 1h. Wash well in dH20 and process into paraffin. I antigen retrieve in DAKO Target Retrieval Solution for 10 min at 95-99 degC in veggie steamer with 10 min cool down. ? Frozen sections for immunofluorescence?are fixed in 4% PFA as above.?Then decal in 10% EDTA for 5 days. ?30% sucrose protect overnight at 4 deg C and snap freeze in OCT. ? It works well with both protocols. ? For both frozens and paraffin, primary is at 2-4 ug/ml followed by very specific biotinylated secondary against goat IgG (Jackson Immunoresearch, minimally cross reactive to other species). Then use strep-HRP (Jackson IR)?and DAB+ (DAKO) to develop. Please let me know if you need more details. ? Best, Andrea Hooper ? --- On Thu, 11/5/09, julia hough wrote: From: julia hough Subject: [Histonet] IHC on N-cadherin, CD146, CD144,ELF97 TRAcP. To: histonet@lists.utsouthwestern.edu Date: Thursday, November 5, 2009, 3:38 PM Dear histonetters. I am trying to perform several new IHC stains on mouse tibia. However, I am having trouble finding literature on some of them. I would like antibodies that work on mouse tissue/bone that are preferably conjugated to a fluorchrome. If anyone has any potentially protocols or information where I could get primary antibodies unconjugated/conjugated for the following that would be lovely: N-cadherin, CD146, CD144,ELF97 TRAcP. Thanks for your time. Julia ??? ???????? ?????? ??? ? _________________________________________________________________ New Windows 7: Simplify what you do everyday. Find the right PC for you. http://www.microsoft.com/uk/windows/buy/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Mon Feb 22 15:21:40 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Mon Feb 22 15:21:44 2010 Subject: [Histonet] EDTA In-Reply-To: <796184.91241.qm@web65714.mail.ac4.yahoo.com> Message-ID: <724175.12920.qm@web113110.mail.gq1.yahoo.com> Although what you say is true, it is worth noting that there are some antigens/antibodies more amenable to acid decal. I have experienced this myself several times recently?and been burned by thinking EDTA will always give better IHC results. At least for murine bone marrow vasculature antigens, this appears to be the case for some antibodies.? Unfortunately as with most things in histology, there is always exceptions to rules of thumb! --- On Fri, 2/19/10, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] EDTA To: "'histonet@lists.utsouthwestern.edu'" , "Dorothy LWebb" Date: Friday, February 19, 2010, 9:03 PM EDTA (which stands for Ethylene-diamino-tratacetic-acid) is a chelating agent and the method of choice to decalcify BM biopsies. The thing is that it should be used alone, and not combined with any acid, as you state (not even formic acid). By itself will produce an extremely gentle decalcification that will be completely suitable for IHC studies. Ren? J. --- On Fri, 2/19/10, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] EDTA To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, February 19, 2010, 1:29 PM I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix.? I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. Thank you ahead of time for your advice! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Reuel.Cornelia <@t> tsrh.org Mon Feb 22 15:36:24 2010 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Mon Feb 22 15:36:54 2010 Subject: [Histonet] Bone tissue Message-ID: <4B82A478020000C500070ECC@mail.TSRH.ORG> We have a difficulty cutting metatarsal bone . It seems that our sections are so dried up. I was thinking that our dehydration have something to do with this which we have placed it in a wrong processing procedure for our large bone. The tissue is 4 mm thick and 1-2 cm in length and width and was dehydrated in 70% - 4 hrs, 80%-4 hrs,95% -4 hrs and 2 changes of 100% 3 hrs each, paraffin is 4 hrs each 2 changes. The tissue was decalcified in 14% EDTA. When we start cutting them it is so brittle and we could not even create a section. I have surfaced decal it and also place in a softener Mollifex some of it work but some does not work. Please help us remedy this tissue. Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From rjbuesa <@t> yahoo.com Mon Feb 22 15:42:36 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 22 15:42:41 2010 Subject: [Histonet] Bone tissue In-Reply-To: <4B82A478020000C500070ECC@mail.TSRH.ORG> Message-ID: <647472.39750.qm@web65702.mail.ac4.yahoo.com> First of all, Mollifex or any other alkaline substance will do nothing "useful" to the bone. I tend to think that you processed the bone before it was completely decalcified and that is the cause for an?incomplete infiltration and a subsequent difficult sectioning. Ren? J. --- On Mon, 2/22/10, Reuel Cornelia wrote: From: Reuel Cornelia Subject: [Histonet] Bone tissue To: histonet@lists.utsouthwestern.edu Date: Monday, February 22, 2010, 4:36 PM We have a difficulty cutting metatarsal bone . It seems that our sections are so dried up. I was thinking that our dehydration have something to do with this which we have placed it in a wrong processing procedure for our large bone. The tissue is 4 mm thick and 1-2 cm in length and width and? was dehydrated in 70% - 4 hrs, 80%-4 hrs,95% -4 hrs and 2 changes of 100% 3 hrs each, paraffin is 4 hrs each 2 changes. The tissue was decalcified in 14% EDTA. When we start cutting them it is so brittle and we could not even create a section. I have surfaced decal it and also place in a softener Mollifex some of it work but some does not work. Please help us remedy this tissue. Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From esther.peters <@t> verizon.net Mon Feb 22 16:36:39 2010 From: esther.peters <@t> verizon.net (Esther Peters) Date: Mon Feb 22 16:36:43 2010 Subject: [Histonet] DRGs and Histogel In-Reply-To: <946040.14027.qm@web83916.mail.sp1.yahoo.com> References: <37E4BAC017F57141AF64FAA5AEB04CE8033A7A0D@wlmmsx01.nemours.org> <001901cab3e0$a9301fb0$fb905f10$@edu> <946040.14027.qm@web83916.mail.sp1.yahoo.com> Message-ID: <4B8306F7.5040800@verizon.net> I have had the exact same results, one piece processing well and another in the same cassette shrinking and hardening, when using 1.5-2% low melting point agarose (NuSieve) instead of Histogel. I was thinking of changing to Histogel, but then this thread started last summer and now I see issues continue. I would love to know the best way to do this also! Esther Peters, Ph.D. George Mason University dusko trajkovic wrote: I also think that it is strange of the way Histogel processes. I have posted on the Histonet previously about this exact problem. I worked with Jennifer Hofec ker when she was at Vanderbilt U.(sent her my Histogel and she sent me hers) an d ended up with perfectly processed Histogel blocks at our facility and hers. I processed a couple of blocks last week and they were just terrible. No change in the processing schedule, or the way Histogel was liquefied (placed in hot wa ter that was heated in the microwave). Prior to the last two blocks, I must hav e processed at least a dozen blocks without any problems. There was an incident where I placed two histogels in the same cassete. One processed beautifuly and the other was all shrunken and dried up. I do not liquefy the entire tube, rather I scoop out the approximate amount tha t I need and transfer to another tube to heat up. If there is anyone out there in Histoland that has not had any issues with the Histogel, can you please post your procedure on liquefying the Histogel, method of cooling/solidifying and p rocessing schedule? The only thing that I do that is not exact is I do not know the temp of my hot water when i place the Histogel to liquefy. I basically hav e to wait several minutes for the gel to melt and I use it immediately. Any new information or solution from anyone, would be greatly appreciated. Thank you Dusko Trajkovic ________________________________ From: Amy Porter [1] To: Andrea Grantham [2]; HISTONET [3] Sent: Mon, February 22, 2010 9:01:22 AM Subject: RE: [Histonet] DRGs I think it is strange that we are all doing similar techniques and wind up with different outcomes using the histogel. I would be curious how many of us are using the equipment sold with the histogel for warming and cooling opposed to any of us who don't. we did not purchase the equipment and I wonder sometimes if warming the histogel using other means causes some type of breakdown / and do any of you repeatedly reheat the same tube once it has been warmed and resolidified?? -----Original Message----- From: [4]histonet-bounces@lists.utsouthwestern.edu [[5]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Monday, February 22, 2010 9:41 AM To: HISTONET Subject: Re: [Histonet] DRGs Importance: High Hi Carol, I have used histogel for these kinds of samples and also other small, thin tissues like insect antennae and insect GI tracts and midguts. Since I get all my projects already fixed in whatever fixative the investigator chooses, rinsed and placed in 70% ETOH the histogel never touches formalin. I don't use formalin on my processor but start in 70%. I've never had a problem with the histogel. We just put the sample in the histogel flat and stand it up (turn 90?) when embedding in paraffin. I use tissue prep for embedding. If you don't want to use histogel you could try to put the drg's on GN Metricel membrane disc filters. We do this with a lot of the samples I receive, actually I have the investigators or their techs do this. The tissue sticks to the membrane and orientation is a dream. The membrane presents no problem when sectioning. You can get it from VWR. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 [6]algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Feb 18, 2010, at 11:23 AM, Barone, Carol wrote: Histonetter's...we received a "boat-load" of mouse DRGs that had been prepared in histogel and are cutting...well..not so good. We normally do DRGs from FS and get beautiful results. We have used histogel before with other small sample and have never had issues... not sure if it is the Histogel or DRG's (fixed in 10% NBF and then transferred to 70% before placed into the histogel).is the issue..I seem to remember that histogel requires formalin and wonder if the transfer to 70% is causing our problem ...but, obviously there is not much room for error with such tiny- tiny samples and they are already process and in paraffin? I am not quite sure how twe can improve the ones that came in histogel, and were processed to paraffin a paraffin block....any idea's? any experience? any anything? Thx- ASAP! [7]cbarone@nemours.org _______________________________________________ Histonet mailing list [8]Histonet@lists.utsouthwestern.edu [9]http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [10]Histonet@lists.utsouthwestern.edu [11]http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [12]Histonet@lists.utsouthwestern.edu [13]http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [14]Histonet@lists.utsouthwestern.edu [15]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. mailto:portera@msu.edu 2. mailto:algranth@email.arizona.edu 3. mailto:histonet@lists.utsouthwestern.edu 4. mailto:histonet-bounces@lists.utsouthwestern.edu 5. mailto:histonet-bounces@lists.utsouthwestern.edu 6. mailto:algranth@email.arizona.edu 7. mailto:cbarone@nemours.org 8. mailto:Histonet@lists.utsouthwestern.edu 9. http://lists.utsouthwestern.edu/mailman/listinfo/histonet 10. mailto:Histonet@lists.utsouthwestern.edu 11. http://lists.utsouthwestern.edu/mailman/listinfo/histonet 12. mailto:Histonet@lists.utsouthwestern.edu 13. http://lists.utsouthwestern.edu/mailman/listinfo/histonet 14. mailto:Histonet@lists.utsouthwestern.edu 15. http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vaughn.Fierke <@t> va.gov Mon Feb 22 17:01:08 2010 From: Vaughn.Fierke <@t> va.gov (Fierke, Vaughn ) Date: Mon Feb 22 17:01:12 2010 Subject: [Histonet] Work Load Units Message-ID: Looking for a good system that works in recording Work Load Units. I've inquired to CAP; they do not have any material available at this time nor recommendations. I've looked at CPT codes but they only reflect billable services in pathology; descriptions are fairly general and cannot be broken down into tasks of the tech. Looked in the Histonet archives; found information not too current. Thanks for any information. Vaughn From NMitchell <@t> smgnj.com Mon Feb 22 17:09:39 2010 From: NMitchell <@t> smgnj.com (Mitchell, Nancy) Date: Mon Feb 22 17:09:47 2010 Subject: [Histonet] Softening nails Message-ID: Rene- Can you share your nail softening technique with all of us? Merci. This e-mail may contain confidential or privileged information. If you are not the intended recipient, please advise the sender by reply e-mail and then delete this e-mail immediately. From tkngflght <@t> yahoo.com Mon Feb 22 17:11:51 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Feb 22 17:11:55 2010 Subject: [Histonet] best of the nail procedures Message-ID: <216045.7759.qm@web50907.mail.re2.yahoo.com> The general idea is the extreme base breaks the links in keratin.? I tried all of Rene's variations and selected 20% KOH. ? Well fixed nails (NBF) Soak in 20% KOH for 30-60 minutes (checking at 10 minute intervals) until soft and pliable. (if you let it go to long it turns into mush) Rinse well. Process Embed Cut and stain...PASF works beautifully. ? ? May want to run a parallel on split specimens before switching entirely to acclimate. (identifying?the end point without having bunches of your specimens turn to mush). ? Cheryl ? From Rick.Garnhart <@t> memorialhealthsystem.com Mon Feb 22 17:12:20 2010 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Mon Feb 22 17:12:23 2010 Subject: [Histonet] PPE's embedding and cutting In-Reply-To: Message-ID: Anyone in histology land required to wear all PPE's to embed and cut? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From liz <@t> premierlab.com Mon Feb 22 17:20:28 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Feb 22 17:20:32 2010 Subject: [Histonet] PPE's embedding and cutting In-Reply-To: Message-ID: We wear gloves to section, not necessarily for safety reasons but to eliminate squamous cells on the slides. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rick.Garnhart@memorialhealthsystem.com Sent: Monday, February 22, 2010 4:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PPE's embedding and cutting Anyone in histology land required to wear all PPE's to embed and cut? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Mon Feb 22 17:48:51 2010 From: portera <@t> msu.edu (Amy Porter) Date: Mon Feb 22 17:48:57 2010 Subject: [Histonet] PPE's embedding and cutting In-Reply-To: References: Message-ID: <000001cab419$9626aea0$c2740be0$@edu> Usually only if you are working with prions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rick.Garnhart@memorialhealthsystem.com Sent: Monday, February 22, 2010 6:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PPE's embedding and cutting Anyone in histology land required to wear all PPE's to embed and cut? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.reilly <@t> jcu.edu.au Mon Feb 22 17:56:15 2010 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Mon Feb 22 17:56:24 2010 Subject: [Histonet] Conference in Australia Message-ID: <9D8F229159A6408E8C4A0CEBDFA5F3BD@ad.jcu.edu.au> Dear Histonetters, The Histotechnology Group of Queensland is holding their conference in conjunction with AIMS (Australian Institute of Medical Scientists) on the weekend of June 11,12, 13. 2010 in Townsville, in tropical Queensland, Australia. This is our winter, but in the tropics winter means sunny days and average maximum temperatures of about 26C (74F) Please explore this website for more detail and note that there is still a "call for abstracts" current. http://aims.iamevents.com.au/index.php Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 From pverbrugghe <@t> meddent.uwa.edu.au Mon Feb 22 20:41:33 2010 From: pverbrugghe <@t> meddent.uwa.edu.au (Phebe Verbrugghe) Date: Mon Feb 22 20:41:40 2010 Subject: [Histonet] Double labeling with antibodies that need different fixatives Message-ID: <83FCA9E681495540A80ECBBAEF28B074F32A38@cooper.meddent.uwa.edu.au> Hi all, I would like to do an immunofluorescent double labeling with two antibodies but 1 antibody works on acetone fixed frozen tissue but not on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) and the other one works on formalin fixed paraffin embedded tissue but not on acetone fixed frozen tissue. Is there any way I could still do a double labeling and how? Also, does anyone have experience with zinc fixative? If my antibody works on formalin fixed tissue is it likely to also work on zinc fixed tissue? Thank you very much in advance, Phebe From anonwums1 <@t> gmail.com Mon Feb 22 20:51:37 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Mon Feb 22 20:51:43 2010 Subject: [Histonet] Double labeling with antibodies that need different fixatives In-Reply-To: <83FCA9E681495540A80ECBBAEF28B074F32A38@cooper.meddent.uwa.edu.au> References: <83FCA9E681495540A80ECBBAEF28B074F32A38@cooper.meddent.uwa.edu.au> Message-ID: <858249121002221851v210c3f9dsc2c3ffc2a8039776@mail.gmail.com> Although I haven't tried it myself, others have gotten CD31 from BD to work on FFPE tissue using the tyramide amplification system on zinc buffered formalin fixed sections. I've generally had good luck with zinc buffered formalin myself for many antigens so it may work for your other one. See http://www.ncbi.nlm.nih.gov/pubmed/19052548 Just to be clear, they used zinc buffered formalin, which isn't the same thing as zinc fixative. Adam On Mon, Feb 22, 2010 at 8:41 PM, Phebe Verbrugghe < pverbrugghe@meddent.uwa.edu.au> wrote: > > Hi all, > > I would like to do an immunofluorescent double labeling with two > antibodies but 1 antibody works on acetone fixed frozen tissue but not > on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) > and the other one works on formalin fixed paraffin embedded tissue but > not on acetone fixed frozen tissue. Is there any way I could still do a > double labeling and how? > Also, does anyone have experience with zinc fixative? If my antibody > works on formalin fixed tissue is it likely to also work on zinc fixed > tissue? > > Thank you very much in advance, > > Phebe > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tifei <@t> foxmail.com Mon Feb 22 21:09:11 2010 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Mon Feb 22 21:39:56 2010 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIERvdWJsZSBsYWJlbGluZyB3aXRoIGFudGlib2RpZXMgdGhhdCBuZWVkIGRpZmZlcmVudGZpeGF0aXZlcw==?= References: <83FCA9E681495540A80ECBBAEF28B074F32A38@cooper.meddent.uwa.edu.au>, <858249121002221851v210c3f9dsc2c3ffc2a8039776@mail.gmail.com> Message-ID: <201002231109101359724@foxmail.com> what is the other marker than CD31? May you let us know? 2010-02-23 TF ???? Adam . ????? 2010-02-23 10:55:18 ???? Phebe Verbrugghe ??? histonet ??? Re: [Histonet] Double labeling with antibodies that need differentfixatives Although I haven't tried it myself, others have gotten CD31 from BD to work on FFPE tissue using the tyramide amplification system on zinc buffered formalin fixed sections. I've generally had good luck with zinc buffered formalin myself for many antigens so it may work for your other one. See http://www.ncbi.nlm.nih.gov/pubmed/19052548 Just to be clear, they used zinc buffered formalin, which isn't the same thing as zinc fixative. Adam On Mon, Feb 22, 2010 at 8:41 PM, Phebe Verbrugghe < pverbrugghe@meddent.uwa.edu.au> wrote: > > Hi all, > > I would like to do an immunofluorescent double labeling with two > antibodies but 1 antibody works on acetone fixed frozen tissue but not > on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) > and the other one works on formalin fixed paraffin embedded tissue but > not on acetone fixed frozen tissue. Is there any way I could still do a > double labeling and how? > Also, does anyone have experience with zinc fixative? If my antibody > works on formalin fixed tissue is it likely to also work on zinc fixed > tissue? > > Thank you very much in advance, > > Phebe > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pverbrugghe <@t> meddent.uwa.edu.au Mon Feb 22 21:45:48 2010 From: pverbrugghe <@t> meddent.uwa.edu.au (Phebe Verbrugghe) Date: Mon Feb 22 21:45:54 2010 Subject: [Histonet] Double labeling with antibodies that need different fixatives In-Reply-To: <858249121002221851v210c3f9dsc2c3ffc2a8039776@mail.gmail.com> References: <83FCA9E681495540A80ECBBAEF28B074F32A38@cooper.meddent.uwa.edu.au> <858249121002221851v210c3f9dsc2c3ffc2a8039776@mail.gmail.com> Message-ID: <83FCA9E681495540A80ECBBAEF28B074F32A3A@cooper.meddent.uwa.edu.au> Hello Adam, Thank you very much for this very useful information! Do you know whether this would also work on tissue fixed with formalin instead of zinc buffered formalin by any chance? Also, could you give me the recipe for the zinc formalin and can I use a standard tissue processor for embedding in paraffin or should I use a specific protocol manually and if so, which? Thanks! Phebe ________________________________ From: Adam . [mailto:anonwums1@gmail.com] Sent: Tuesday, 23 February 2010 10:52 AM To: Phebe Verbrugghe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives Although I haven't tried it myself, others have gotten CD31 from BD to work on FFPE tissue using the tyramide amplification system on zinc buffered formalin fixed sections. I've generally had good luck with zinc buffered formalin myself for many antigens so it may work for your other one. See http://www.ncbi.nlm.nih.gov/pubmed/19052548 Just to be clear, they used zinc buffered formalin, which isn't the same thing as zinc fixative. Adam On Mon, Feb 22, 2010 at 8:41 PM, Phebe Verbrugghe wrote: Hi all, I would like to do an immunofluorescent double labeling with two antibodies but 1 antibody works on acetone fixed frozen tissue but not on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) and the other one works on formalin fixed paraffin embedded tissue but not on acetone fixed frozen tissue. Is there any way I could still do a double labeling and how? Also, does anyone have experience with zinc fixative? If my antibody works on formalin fixed tissue is it likely to also work on zinc fixed tissue? Thank you very much in advance, Phebe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anonwums1 <@t> gmail.com Mon Feb 22 22:00:31 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Mon Feb 22 22:00:36 2010 Subject: [Histonet] Double labeling with antibodies that need different fixatives In-Reply-To: <83FCA9E681495540A80ECBBAEF28B074F32A3A@cooper.meddent.uwa.edu.au> References: <83FCA9E681495540A80ECBBAEF28B074F32A38@cooper.meddent.uwa.edu.au> <858249121002221851v210c3f9dsc2c3ffc2a8039776@mail.gmail.com> <83FCA9E681495540A80ECBBAEF28B074F32A3A@cooper.meddent.uwa.edu.au> Message-ID: <858249121002222000tf00dadel10fc736700e97b57@mail.gmail.com> I have no idea if it would work with regular formalin. In my experience, many antigens work as well in zinc buffered formalin without any antigen retrieval as regular formalin with antigen retrieval. But really, you just have to try it yourself. On antibodies I've gotten this to work, I use commercially available zinc buffered formalin which comes in gallon jugs for around $50. We don't do any special processing. We plop our sample (bones) in zinc formalin overnight at 4C, decalcify in EDTA or formic acid (only necessary for bones), and then embed just like any other sample. Adam On Mon, Feb 22, 2010 at 9:45 PM, Phebe Verbrugghe < pverbrugghe@meddent.uwa.edu.au> wrote: > Hello Adam, > > Thank you very much for this very useful information! > Do you know whether this would also work on tissue fixed with formalin > instead of zinc buffered formalin by any chance? > Also, could you give me the recipe for the zinc formalin and can I use a > standard tissue processor for embedding in paraffin or should I use a > specific protocol manually and if so, which? > > Thanks! > > Phebe > > ------------------------------ > *From:* Adam . [mailto:anonwums1@gmail.com] > *Sent:* Tuesday, 23 February 2010 10:52 AM > *To:* Phebe Verbrugghe > *Cc:* histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Double labeling with antibodies that need > different fixatives > > Although I haven't tried it myself, others have gotten CD31 from BD to work > on FFPE tissue using the tyramide amplification system on zinc buffered > formalin fixed sections. I've generally had good luck with zinc buffered > formalin myself for many antigens so it may work for your other one. > > See http://www.ncbi.nlm.nih.gov/pubmed/19052548 > > Just to be clear, they used zinc buffered formalin, which isn't the same > thing as zinc fixative. > > Adam > > On Mon, Feb 22, 2010 at 8:41 PM, Phebe Verbrugghe < > pverbrugghe@meddent.uwa.edu.au> wrote: > >> >> Hi all, >> >> I would like to do an immunofluorescent double labeling with two >> antibodies but 1 antibody works on acetone fixed frozen tissue but not >> on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) >> and the other one works on formalin fixed paraffin embedded tissue but >> not on acetone fixed frozen tissue. Is there any way I could still do a >> double labeling and how? >> Also, does anyone have experience with zinc fixative? If my antibody >> works on formalin fixed tissue is it likely to also work on zinc fixed >> tissue? >> >> Thank you very much in advance, >> >> Phebe >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From pverbrugghe <@t> meddent.uwa.edu.au Mon Feb 22 22:09:48 2010 From: pverbrugghe <@t> meddent.uwa.edu.au (Phebe Verbrugghe) Date: Mon Feb 22 22:09:56 2010 Subject: [Histonet] Double labeling with antibodies that need different fixatives In-Reply-To: <858249121002222000tf00dadel10fc736700e97b57@mail.gmail.com> References: <83FCA9E681495540A80ECBBAEF28B074F32A38@cooper.meddent.uwa.edu.au> <858249121002221851v210c3f9dsc2c3ffc2a8039776@mail.gmail.com> <83FCA9E681495540A80ECBBAEF28B074F32A3A@cooper.meddent.uwa.edu.au> <858249121002222000tf00dadel10fc736700e97b57@mail.gmail.com> Message-ID: <83FCA9E681495540A80ECBBAEF28B074F32A3C@cooper.meddent.uwa.edu.au> Hi Adam, Thanks a lot, might just give it a go on just formalin fixed tissue first. Phebe ________________________________ From: Adam . [mailto:anonwums1@gmail.com] Sent: Tuesday, 23 February 2010 12:01 PM To: Phebe Verbrugghe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives I have no idea if it would work with regular formalin. In my experience, many antigens work as well in zinc buffered formalin without any antigen retrieval as regular formalin with antigen retrieval. But really, you just have to try it yourself. On antibodies I've gotten this to work, I use commercially available zinc buffered formalin which comes in gallon jugs for around $50. We don't do any special processing. We plop our sample (bones) in zinc formalin overnight at 4C, decalcify in EDTA or formic acid (only necessary for bones), and then embed just like any other sample. Adam On Mon, Feb 22, 2010 at 9:45 PM, Phebe Verbrugghe wrote: Hello Adam, Thank you very much for this very useful information! Do you know whether this would also work on tissue fixed with formalin instead of zinc buffered formalin by any chance? Also, could you give me the recipe for the zinc formalin and can I use a standard tissue processor for embedding in paraffin or should I use a specific protocol manually and if so, which? Thanks! Phebe ________________________________ From: Adam . [mailto:anonwums1@gmail.com] Sent: Tuesday, 23 February 2010 10:52 AM To: Phebe Verbrugghe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives Although I haven't tried it myself, others have gotten CD31 from BD to work on FFPE tissue using the tyramide amplification system on zinc buffered formalin fixed sections. I've generally had good luck with zinc buffered formalin myself for many antigens so it may work for your other one. See http://www.ncbi.nlm.nih.gov/pubmed/19052548 Just to be clear, they used zinc buffered formalin, which isn't the same thing as zinc fixative. Adam On Mon, Feb 22, 2010 at 8:41 PM, Phebe Verbrugghe wrote: Hi all, I would like to do an immunofluorescent double labeling with two antibodies but 1 antibody works on acetone fixed frozen tissue but not on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) and the other one works on formalin fixed paraffin embedded tissue but not on acetone fixed frozen tissue. Is there any way I could still do a double labeling and how? Also, does anyone have experience with zinc fixative? If my antibody works on formalin fixed tissue is it likely to also work on zinc fixed tissue? Thank you very much in advance, Phebe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Mon Feb 22 22:47:17 2010 From: tifei <@t> foxmail.com (TF) Date: Mon Feb 22 22:47:45 2010 Subject: [Histonet] Double labeling with antibodies that need differentfixatives References: <83FCA9E681495540A80ECBBAEF28B074F32A38@cooper.meddent.uwa.edu.au>, <858249121002221851v210c3f9dsc2c3ffc2a8039776@mail.gmail.com>, <83FCA9E681495540A80ECBBAEF28B074F32A3A@cooper.meddent.uwa.edu.au> Message-ID: <201002231247163123759@foxmail.com> RnJvbToiQW5hdGVjaCBMdGQuIiA8YW5hdGVjaEBuZXQtbGluay5uZXQ+DQpUbzpIaXN0b05ldEBQ YXRob2xvZ3kuc3dtZWQuZWR1DQpSZXBseS1UbzoNCkRhdGU6TW9uLCAyMSBKdW4gMTk5OSAxMjox MjoxOSAtMDQwMA0KQ29udGVudC1UeXBlOnRleHQvcGxhaW47IGNoYXJzZXQ9InVzLWFzY2lpIg0K DQoNCg0KQSBjb21wcmVoZW5zaXZlIHJldmlldyBvZiB6aW5jIGZvcm1hbGluIHVwIHRvIDE5OTIg 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eQ0KYW50aWJvZHkNCndvcmtzIG9uIGZvcm1hbGluIGZpeGVkIHRpc3N1ZSBpcyBpdCBsaWtlbHkg dG8gYWxzbyB3b3JrIG9uIHppbmMNCmZpeGVkDQp0aXNzdWU/DQpUaGFuayB5b3UgdmVyeSBtdWNo IGluIGFkdmFuY2UsDQpQaGViZQ0KX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX18NCkhpc3RvbmV0IG1haWxpbmcgbGlzdA0KSGlzdG9uZXRAbGlzdHMudXRzb3V0 aHdlc3Rlcm4uZWR1DQpodHRwOi8vbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1L21haWxtYW4vbGlz dGluZm8vaGlzdG9uZXQNCl9fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fDQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0 ZXJuLmVkdQ0KaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZv L2hpc3RvbmV0DQo= From Tony_Reilly <@t> health.qld.gov.au Mon Feb 22 23:47:13 2010 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Mon Feb 22 23:50:39 2010 Subject: [Histonet] Double labeling with antibodies that need differentfixatives In-Reply-To: <83FCA9E681495540A80ECBBAEF28B074F32A3C@cooper.meddent.uwa.edu.au> References: <83FCA9E681495540A80ECBBAEF28B074F32A38@cooper.meddent.uwa.edu.au> <858249121002221851v210c3f9dsc2c3ffc2a8039776@mail.gmail.com> <83FCA9E681495540A80ECBBAEF28B074F32A3A@cooper.meddent.uwa.edu.au> <858249121002222000tf00dadel10fc736700e97b57@mail.gmail.com> <83FCA9E681495540A80ECBBAEF28B074F32A3C@cooper.meddent.uwa.edu.au> Message-ID: <4B83F880.471C.0039.0@health.qld.gov.au> Hi Phebe The first place to look when working up Antibodies is the Specification sheet from the manufacturer. If you look at the BD sheet for their CD31 Immunohistochemistry is recommended for Zinc fixed paraffin sections and acetone fixed frozeb sections but not recommended for formalin fixed paraffin sections. regards Tony Tony Reilly Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Phebe Verbrugghe" 23/02/2010 2:09 pm >>> Hi Adam, Thanks a lot, might just give it a go on just formalin fixed tissue first. Phebe ________________________________ From: Adam . [mailto:anonwums1@gmail.com] Sent: Tuesday, 23 February 2010 12:01 PM To: Phebe Verbrugghe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives I have no idea if it would work with regular formalin. In my experience, many antigens work as well in zinc buffered formalin without any antigen retrieval as regular formalin with antigen retrieval. But really, you just have to try it yourself. On antibodies I've gotten this to work, I use commercially available zinc buffered formalin which comes in gallon jugs for around $50. We don't do any special processing. We plop our sample (bones) in zinc formalin overnight at 4C, decalcify in EDTA or formic acid (only necessary for bones), and then embed just like any other sample. Adam On Mon, Feb 22, 2010 at 9:45 PM, Phebe Verbrugghe wrote: Hello Adam, Thank you very much for this very useful information! Do you know whether this would also work on tissue fixed with formalin instead of zinc buffered formalin by any chance? Also, could you give me the recipe for the zinc formalin and can I use a standard tissue processor for embedding in paraffin or should I use a specific protocol manually and if so, which? Thanks! Phebe ________________________________ From: Adam . [mailto:anonwums1@gmail.com] Sent: Tuesday, 23 February 2010 10:52 AM To: Phebe Verbrugghe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives Although I haven't tried it myself, others have gotten CD31 from BD to work on FFPE tissue using the tyramide amplification system on zinc buffered formalin fixed sections. I've generally had good luck with zinc buffered formalin myself for many antigens so it may work for your other one. See http://www.ncbi.nlm.nih.gov/pubmed/19052548 Just to be clear, they used zinc buffered formalin, which isn't the same thing as zinc fixative. Adam On Mon, Feb 22, 2010 at 8:41 PM, Phebe Verbrugghe wrote: Hi all, I would like to do an immunofluorescent double labeling with two antibodies but 1 antibody works on acetone fixed frozen tissue but not on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) and the other one works on formalin fixed paraffin embedded tissue but not on acetone fixed frozen tissue. Is there any way I could still do a double labeling and how? Also, does anyone have experience with zinc fixative? If my antibody works on formalin fixed tissue is it likely to also work on zinc fixed tissue? Thank you very much in advance, Phebe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From Tony_Reilly <@t> health.qld.gov.au Tue Feb 23 00:00:39 2010 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Tue Feb 23 00:17:19 2010 Subject: [Histonet] Double labeling with antibodies that need differentfixatives In-Reply-To: <83FCA9E681495540A80ECBBAEF28B074F32A3C@cooper.meddent.uwa.edu.au> References: <83FCA9E681495540A80ECBBAEF28B074F32A38@cooper.meddent.uwa.edu.au> <858249121002221851v210c3f9dsc2c3ffc2a8039776@mail.gmail.com> <83FCA9E681495540A80ECBBAEF28B074F32A3A@cooper.meddent.uwa.edu.au> <858249121002222000tf00dadel10fc736700e97b57@mail.gmail.com> <83FCA9E681495540A80ECBBAEF28B074F32A3C@cooper.meddent.uwa.edu.au> Message-ID: <4B83FBA6.471C.0039.0@health.qld.gov.au> Hi Phebe The first place to look when working up Antibodies is the Specification sheet from the manufacturer. If you look at the BD sheet for their CD31 Immunohistochemistry is recommended for Zinc fixed paraffin sections and acetone fixed frozeb sections but not recommended for formalin fixed paraffin sections. regards Tony Tony Reilly Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Phebe Verbrugghe" 23/02/2010 2:09 pm >>> Hi Adam, Thanks a lot, might just give it a go on just formalin fixed tissue first. Phebe ________________________________ From: Adam . [mailto:anonwums1@gmail.com] Sent: Tuesday, 23 February 2010 12:01 PM To: Phebe Verbrugghe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives I have no idea if it would work with regular formalin. In my experience, many antigens work as well in zinc buffered formalin without any antigen retrieval as regular formalin with antigen retrieval. But really, you just have to try it yourself. On antibodies I've gotten this to work, I use commercially available zinc buffered formalin which comes in gallon jugs for around $50. We don't do any special processing. We plop our sample (bones) in zinc formalin overnight at 4C, decalcify in EDTA or formic acid (only necessary for bones), and then embed just like any other sample. Adam On Mon, Feb 22, 2010 at 9:45 PM, Phebe Verbrugghe wrote: Hello Adam, Thank you very much for this very useful information! Do you know whether this would also work on tissue fixed with formalin instead of zinc buffered formalin by any chance? Also, could you give me the recipe for the zinc formalin and can I use a standard tissue processor for embedding in paraffin or should I use a specific protocol manually and if so, which? Thanks! Phebe ________________________________ From: Adam . [mailto:anonwums1@gmail.com] Sent: Tuesday, 23 February 2010 10:52 AM To: Phebe Verbrugghe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives Although I haven't tried it myself, others have gotten CD31 from BD to work on FFPE tissue using the tyramide amplification system on zinc buffered formalin fixed sections. I've generally had good luck with zinc buffered formalin myself for many antigens so it may work for your other one. See http://www.ncbi.nlm.nih.gov/pubmed/19052548 Just to be clear, they used zinc buffered formalin, which isn't the same thing as zinc fixative. Adam On Mon, Feb 22, 2010 at 8:41 PM, Phebe Verbrugghe wrote: Hi all, I would like to do an immunofluorescent double labeling with two antibodies but 1 antibody works on acetone fixed frozen tissue but not on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) and the other one works on formalin fixed paraffin embedded tissue but not on acetone fixed frozen tissue. Is there any way I could still do a double labeling and how? Also, does anyone have experience with zinc fixative? If my antibody works on formalin fixed tissue is it likely to also work on zinc fixed tissue? Thank you very much in advance, Phebe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From ree3 <@t> leicester.ac.uk Tue Feb 23 03:37:45 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Tue Feb 23 03:37:52 2010 Subject: [Histonet] FW: mouse prions Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8CBD35735@EXC-MBX3.cfs.le.ac.uk> Anybody out there handling/processing/sectioning mouse tissue containing mouse prions, if so, what safety precautions do you take?. Thanks Richard Edwards From JSilverman <@t> NSHS.edu Tue Feb 23 08:23:39 2010 From: JSilverman <@t> NSHS.edu (Silverman, Jeffrey) Date: Tue Feb 23 08:23:56 2010 Subject: [Histonet] New cryostat decontamination CAP statndard Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE2BF9BCF@SYKECHXVS01.nslijhs.net> Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP.12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. --------------------------------------------------------------------------------- The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. From rjbuesa <@t> yahoo.com Tue Feb 23 08:47:26 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 23 08:47:33 2010 Subject: [Histonet] Work Load Units In-Reply-To: Message-ID: <918372.18539.qm@web65711.mail.ac4.yahoo.com> What do you exactly mean by "Work Load Units"? You should know how many cases and blocks you process by day, week or year, and you also know how many hours your staff uses to complete the tasks derived from those cases and blocks. The total work completed between the staff members completing them during a given amount of time will allow you to calculate productivity. If you are referring to which to select, the case?is relevant? for the pathologists, the slide for the cytotech and the block for the histotech because they refer to what the usually handle. Ren? J. --- On Mon, 2/22/10, Fierke, Vaughn wrote: From: Fierke, Vaughn Subject: [Histonet] Work Load Units To: histonet@lists.utsouthwestern.edu Date: Monday, February 22, 2010, 6:01 PM Looking for a good system that works in recording Work Load Units. I've inquired to CAP; they do not have any material available at this time nor recommendations. I've looked at CPT codes but they only reflect billable services in pathology; descriptions are fairly general and cannot be broken down into tasks of the tech. Looked in the Histonet archives; found information not too current. Thanks for any information. Vaughn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 23 08:52:54 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 23 08:52:59 2010 Subject: [Histonet] PPE's embedding and cutting In-Reply-To: Message-ID: <663253.5809.qm@web65716.mail.ac4.yahoo.com> No! Neither to wear gloves when sectioning. Both are unwarranted requirements that interfere with the histotech's productivity. Ren? J. --- On Mon, 2/22/10, Rick.Garnhart@memorialhealthsystem.com wrote: From: Rick.Garnhart@memorialhealthsystem.com Subject: [Histonet] PPE's embedding and cutting To: histonet@lists.utsouthwestern.edu Date: Monday, February 22, 2010, 6:12 PM Anyone in histology land required to wear all PPE's to embed and cut? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph:? 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Tue Feb 23 09:05:58 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Tue Feb 23 09:06:02 2010 Subject: [Histonet] New cryostat decontamination CAP statndard In-Reply-To: <83F4D81747A7094DAFE3AE87151ECB941CE2BF9BCF@SYKECHXVS01.nslijhs.net> Message-ID: <654447032.153941266937558654.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Thank you Jeffery.? I am not sure how we get to CAP on this however; it makes no sense for a really busy lab unless they want to give us enough cryostats to make up for the problem this will cause.? Is this something NSH might decide to help with?? It may be time to bombard them (CAP) with questions and report the issues it will cause with patient care.? None of us want to use a contaminated cryostat but we also know how to decontaminate without being in danger and stopping surgery for frozens . Pam Marcum UAMS - Anatomic Pathology ----- Original Message ----- From: "Jeffrey Silverman " < JSilverman @ NSHS . edu > To: histonet @lists. utsouthwestern . edu Cc: "Jeffrey Silverman " < JSilverman @ NSHS . edu > Sent: Tuesday, February 23, 2010 8:23:39 AM GMT -06:00 US/Canada Central Subject: [ Histonet ] New cryostat decontamination CAP statndard Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand ?both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP .12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. --------------------------------------------------------------------------------- The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet From mpence <@t> grhs.net Tue Feb 23 09:18:20 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Feb 23 09:18:26 2010 Subject: [Histonet] New cryostat decontamination CAP statndard In-Reply-To: <654447032.153941266937558654.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3D73@is-e2k3.grhs.net> The way I read this is if I don't use my cryostat 7 days a week, 365 days a year, then I don't use it daily and therefore I will set my own routine decontamination schedule. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Tuesday, February 23, 2010 9:06 AM To: Jeffrey Silverman Cc: histonet@lists.utsouthwestern.edu; Jeffrey Silverman Subject: Re: [Histonet] New cryostat decontamination CAP statndard Thank you Jeffery.? I am not sure how we get to CAP on this however; it makes no sense for a really busy lab unless they want to give us enough cryostats to make up for the problem this will cause.? Is this something NSH might decide to help with?? It may be time to bombard them (CAP) with questions and report the issues it will cause with patient care.? None of us want to use a contaminated cryostat but we also know how to decontaminate without being in danger and stopping surgery for frozens . Pam Marcum UAMS - Anatomic Pathology ----- Original Message ----- From: "Jeffrey Silverman " < JSilverman @ NSHS . edu > To: histonet @lists. utsouthwestern . edu Cc: "Jeffrey Silverman " < JSilverman @ NSHS . edu > Sent: Tuesday, February 23, 2010 8:23:39 AM GMT -06:00 US/Canada Central Subject: [ Histonet ] New cryostat decontamination CAP statndard Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand ?both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP .12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. --------------------------------------------------------------------------------- The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 23 09:33:42 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 23 09:33:51 2010 Subject: [Histonet] New cryostat decontamination CAP statndard In-Reply-To: <654447032.153941266937558654.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <993341.75275.qm@web65706.mail.ac4.yahoo.com> Please remember that from time to time CAP inspectors sit to ponder "what something new can we add to our list so it appears that we are not using always the same old one?". And from time to time the come up with requirements like this. You will never change them and the requirements will keep piling up one on top of the other. Caveat: sometimes the changes are warranted (only some). Ren? J. ? --- On Tue, 2/23/10, Pamela Marcum wrote: From: Pamela Marcum Subject: Re: [Histonet] New cryostat decontamination CAP statndard To: "Jeffrey Silverman" Cc: histonet@lists.utsouthwestern.edu, "Jeffrey Silverman" Date: Tuesday, February 23, 2010, 10:05 AM Thank you Jeffery.? I am not sure how we get to CAP on this however; it makes no sense for a really busy lab unless they want to give us enough cryostats to make up for the problem this will cause.? Is this something NSH might decide to help with?? It may be time to bombard them (CAP) with questions and report the issues it will cause with patient care.? None of us want to use a contaminated cryostat but we also know how to decontaminate without being in danger and stopping surgery for frozens . Pam Marcum UAMS - Anatomic Pathology ----- Original Message ----- From: "Jeffrey Silverman " < JSilverman @ NSHS . edu > To: histonet @lists. utsouthwestern . edu Cc: "Jeffrey Silverman " < JSilverman @ NSHS . edu > Sent: Tuesday, February 23, 2010 8:23:39 AM GMT -06:00 US/Canada Central Subject: [ Histonet ] New cryostat decontamination CAP statndard Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand ?both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP .12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. --------------------------------------------------------------------------------- The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Feb 23 09:36:26 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Feb 23 09:36:32 2010 Subject: [Histonet] Work Load Units In-Reply-To: <918372.18539.qm@web65711.mail.ac4.yahoo.com> References: <918372.18539.qm@web65711.mail.ac4.yahoo.com> Message-ID: <27648A6C5BC9B145813DF5182F83AB450410ED@ITSSSXM01V1.one.ads.che.org> Unfortunately, many facilities are only counting billable tests and all the recuts, sendouts, and work done pulling cases for conferences cannot be counted. It is a nightmare that needs to be addressed!! My 2 cents...j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 23, 2010 09:47 To: histonet@lists.utsouthwestern.edu; VaughnFierke Subject: Re: [Histonet] Work Load Units What do you exactly mean by "Work Load Units"? You should know how many cases and blocks you process by day, week or year, and you also know how many hours your staff uses to complete the tasks derived from those cases and blocks. The total work completed between the staff members completing them during a given amount of time will allow you to calculate productivity. If you are referring to which to select, the case?is relevant? for the pathologists, the slide for the cytotech and the block for the histotech because they refer to what the usually handle. Ren? J. --- On Mon, 2/22/10, Fierke, Vaughn wrote: From: Fierke, Vaughn Subject: [Histonet] Work Load Units To: histonet@lists.utsouthwestern.edu Date: Monday, February 22, 2010, 6:01 PM Looking for a good system that works in recording Work Load Units. I've inquired to CAP; they do not have any material available at this time nor recommendations. I've looked at CPT codes but they only reflect billable services in pathology; descriptions are fairly general and cannot be broken down into tasks of the tech. Looked in the Histonet archives; found information not too current. Thanks for any information. Vaughn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From madelinegi <@t> yahoo.com Tue Feb 23 10:08:41 2010 From: madelinegi <@t> yahoo.com (Madeline Gi) Date: Tue Feb 23 10:08:44 2010 Subject: [Histonet] (no subject) Message-ID: <791734.89284.qm@web59810.mail.ac4.yahoo.com> madelinegi@yahoo.com ? Madeline Rotger Milanese H.T. 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com From JEllin <@t> yumaregional.org Tue Feb 23 10:27:40 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Feb 23 10:27:49 2010 Subject: [Histonet] New cryostat decontamination CAP statndard In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3D73@is-e2k3.grhs.net> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C606E@EXCHANGECLUSTER.yumaregional.local> To me the key word in here is interval. If a regularly scheduled interval is set, this could be daily , weekly, monthly or quarterly. Obviously there are going to be the times when a contaminant is there and the machine has to be brought down, but also I do not see the mention of the UV light. We have a Leica that has an internal UV button. This is pressed everyday as well as normal daily maintenance. Once a month our cryostat is brought down for decontamination. This is part of the normal maintenance. I agree that is the CAP wants this to happen, it needs to be clearly defined within the question and not up to interpretation. Jesus Ellin HT/PA ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, February 23, 2010 8:18 AM To: Pamela Marcum; Jeffrey Silverman Cc: histonet@lists.utsouthwestern.edu; Jeffrey Silverman Subject: RE: [Histonet] New cryostat decontamination CAP statndard The way I read this is if I don't use my cryostat 7 days a week, 365 days a year, then I don't use it daily and therefore I will set my own routine decontamination schedule. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Tuesday, February 23, 2010 9:06 AM To: Jeffrey Silverman Cc: histonet@lists.utsouthwestern.edu; Jeffrey Silverman Subject: Re: [Histonet] New cryostat decontamination CAP statndard Thank you Jeffery.? I am not sure how we get to CAP on this however; it makes no sense for a really busy lab unless they want to give us enough cryostats to make up for the problem this will cause.? Is this something NSH might decide to help with?? It may be time to bombard them (CAP) with questions and report the issues it will cause with patient care.? None of us want to use a contaminated cryostat but we also know how to decontaminate without being in danger and stopping surgery for frozens . Pam Marcum UAMS - Anatomic Pathology ----- Original Message ----- From: "Jeffrey Silverman " < JSilverman @ NSHS . edu > To: histonet @lists. utsouthwestern . edu Cc: "Jeffrey Silverman " < JSilverman @ NSHS . edu > Sent: Tuesday, February 23, 2010 8:23:39 AM GMT -06:00 US/Canada Central Subject: [ Histonet ] New cryostat decontamination CAP statndard Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand ?both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP .12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. --------------------------------------------------------------------------------- The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From jclark <@t> pcnm.com Tue Feb 23 10:54:11 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Tue Feb 23 10:54:16 2010 Subject: [Histonet] Workload units Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C012066E4@mail.pcnm.com> I am a Canadian trained tech and back home we used to have to record workload units that had to be reported to the Ministry of Health. The Ministry set a standard of one workload unit per minute and a study was conducted to calculate the amount of time it took a tech to complete a task/test and so all tests were assigned a workload value. For example, an H&E was worth one test and seven units. So Rene is correct in how to come up with your values, but don't forget the non testing tasks that also need to be carried out such as doing your QC, filing blocks, changing the processors etc. All these things take time, but are not considered a 'test'. We would use our workload units to validate to administration our need for more staff. The justification was that if it took one minute to generate one workload unit, than a tech could not safely do more than 60 units per hour. When the workload units became more than what the number of your staff should be producing for a set period of time, than you were risking patient safety. Until ASCP or an equivalent lab governing body come up with the definition of a workload unit, coming up with your own might be a moot point. Joanne Clark, HT Histology Supervisor Path Consultants of New Mexico Roswell ------------------------------ Message: 1 Date: Mon, 22 Feb 2010 15:01:08 -0800 From: "Fierke, Vaughn " Subject: [Histonet] Work Load Units To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Looking for a good system that works in recording Work Load Units. I've inquired to CAP; they do not have any material available at this time nor recommendations. I've looked at CPT codes but they only reflect billable services in pathology; descriptions are fairly general and cannot be broken down into tasks of the tech. Looked in the Histonet archives; found information not too current. Thanks for any information. Vaughn From kelleydurden <@t> pathology.ufl.edu Tue Feb 23 11:23:31 2010 From: kelleydurden <@t> pathology.ufl.edu (Durden, Kelley) Date: Tue Feb 23 11:24:11 2010 Subject: [Histonet] seeking histology position - Ron Martin Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE57B9C06C@HSC-CMS01.ad.ufl.edu> Go to https://jobs.ufl.edu/applicants/jsp/shared/frameset/Frameset.jsp?time=1266864723194 You'll have to create a user ID and login. UF will have a posting in the next week or so. ASCP license desired, FL DOH license is a plus. Also the Gainesville Veterans hospital has a position available - you can apply in person but I am not sure where to apply online. I hope you are successful in your job search! Kelley From JSilverman <@t> NSHS.edu Tue Feb 23 11:35:55 2010 From: JSilverman <@t> NSHS.edu (Silverman, Jeffrey) Date: Tue Feb 23 11:36:13 2010 Subject: [Histonet] New cryostat decontamination CAP statndard In-Reply-To: <4B838B41.4347.0054.0@ah.org> References: <83F4D81747A7094DAFE3AE87151ECB941CE2BF9BCF@SYKECHXVS01.nslijhs.net> <4B838B41.4347.0054.0@ah.org> Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE2BF9BD0@SYKECHXVS01.nslijhs.net> Folks: Please read the checklist notes carefully- I don't think for most of us this is open to interpretation. The standard note says: MUST be defrosted and decontaminated with a tuberculocidal WEEKLY for machines used daily. It's not open to interpretation if you do frozens daily. Tiny institutions that do one or two frozens a week may have some leeway, but I suspect most of us need to do it weekly to comply with the standard. And the UV lights are not tuberculocidal and do not reach all surfaces. See this link from the Leica instruction manual: http://www.well.ox.ac.uk/_asset/file/leica-disinifection-2.pdf. A partial quote: Please note that ALL visible contamination must be removed from the chamber before using the UV C light and that the germicidal effect of the radiation is restricted to directly illuminated areas. Exposure to UV irradiation cannot replace chemical disinfection. 5. For more extensive disinfection, bring the cryostat to room temperature and wipe the exposed surfaces with an EPA approved tuberculocidal disinfectant. The EPA publishes a list of approved disinfectants. Follow the directions written on the containers. Another reference re: UV decon: from -- http://www.unmc.edu/media/ibc/manual/biosafety_manual_march2008_final_compressed.doc#engineering Radiation Gamma and X-ray are two principal types of ionizing radiation used in sterilization. Their application is mainly centered on the sterilization of prepackaged medical devices. Ultraviolet (UV) radiation is a practical method for inactivating viruses, Mycoplasma, bacteria and fungi. UV radiation is successfully used in the destruction of airborne microorganisms; UV light sterilizing capabilities are limited on surfaces because of its lack of penetrating power. Finally, here's an article on biosafety in pathology labs from CAP Today where they reiterate the weekly requirement for cryostat decontamination at room temperature. http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=cap_today%2F0909%2F0909b_taking_stock.html&_state=maximized&_pageLabel=cntvwr I can just hear rhe sounds of numerous CAP "dings" being registered. Oh, the humanity. :-) Jeff Silverman -----Original Message----- From: Behnaz Sohrab [mailto:SohrabB1@ah.org] Sent: Tuesday, February 23, 2010 11:01 AM To: Silverman, Jeffrey Subject: Re: [Histonet] New cryostat decontamination CAP statndard I think we can establish our own QC definition for " ROUTINE" as they are requesting, it could be once a month or any other routine that works for your lab usage and size? Behnaz >>> "Silverman, Jeffrey" 2/23/2010 6:23 AM >>> Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP.12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. --------------------------------------------------------------------------------- The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------------------- The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. From k.fd <@t> live.com Tue Feb 23 12:53:19 2010 From: k.fd <@t> live.com (Karen Doty) Date: Tue Feb 23 12:53:25 2010 Subject: [Histonet] DRGs histogel In-Reply-To: <946040.14027.qm@web83916.mail.sp1.yahoo.com> References: <37E4BAC017F57141AF64FAA5AEB04CE8033A7A0D@wlmmsx01.nemours.org>, , <001901cab3e0$a9301fb0$fb905f10$@edu>, <946040.14027.qm@web83916.mail.sp1.yahoo.com> Message-ID: I think dehydration during processing can effect the outcome with histogel. I use it frequently to help orient small samples with very good results almost always. One time, I was trying to arrange several samples in the same block and the histogel got too cold and set up when I was partly finished. I put it all in our oven hoping to remelt it. When I took it out, it had become a hard thin sheet, sort of like dried paint or glue. The only thing I could think of that had happened, was that since it was in a shape with a lot of surface area, it had dried out instead of melting. I wanted to save the sample that was in it, so I added some water. Thankfully, the histogel became gel like again and I was able to find the sample which I transfered to fresh histogel. The reconstituted histogel seemed to be the same as regular histogel, excep it lost its pink color. The samples also were ok. I've never had any real problems cutting histogel (and hope I don't), but if I get one that is a little crumbly, I soak it extra long and that helps. I think that maybe when there are inconsistent results with histogel, maybe something happens during processing so it becomes dried out or overly dehydrated. I have used it both out of formalin and out of 70%. I've also embedded it both wet and dry without problems. Sometimes it is whiter than others. Karen Doty > Date: Mon, 22 Feb 2010 09:35:10 -0800 > From: dunatrsd@sbcglobal.net > To: portera@msu.edu; algranth@email.arizona.edu; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] DRGs > CC: > > I also think that it is strange of the way Histogel processes. I have posted on the Histonet previously about this exact problem. I worked with Jennifer Hofecker when she was at Vanderbilt U.(sent her my Histogel and she sent me hers) and ended up with perfectly processed Histogel blocks at our facility and hers. I processed a couple of blocks last week and they were just terrible. No change in the processing schedule, or the way Histogel was liquefied (placed in hot water that was heated in the microwave). Prior to the last two blocks, I must have processed at least a dozen blocks without any problems. There was an incident where I placed two histogels in the same cassete. One processed beautifuly and the other was all shrunken and dried up. > I do not liquefy the entire tube, rather I scoop out the approximate amount that I need and transfer to another tube to heat up. If there is anyone out there in Histoland that has not had any issues with the Histogel, can you please post your procedure on liquefying the Histogel, method of cooling/solidifying and processing schedule? The only thing that I do that is not exact is I do not know the temp of my hot water when i place the Histogel to liquefy. I basically have to wait several minutes for the gel to melt and I use it immediately. > Any new information or solution from anyone, would be greatly appreciated. > Thank you > Dusko Trajkovic > > > > ________________________________ > From: Amy Porter > To: Andrea Grantham ; HISTONET > Sent: Mon, February 22, 2010 9:01:22 AM > Subject: RE: [Histonet] DRGs > > I think it is strange that we are all doing similar techniques and wind up > with different outcomes using the histogel. I would be curious how many of > us are using the equipment sold with the histogel for warming and cooling > opposed to any of us who don't. we did not purchase the equipment and I > wonder sometimes if warming the histogel using other means causes some type > of breakdown / and do any of you repeatedly reheat the same tube once it has > been warmed and resolidified?? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea > Grantham > Sent: Monday, February 22, 2010 9:41 AM > To: HISTONET > Subject: Re: [Histonet] DRGs > Importance: High > > Hi Carol, > I have used histogel for these kinds of samples and also other small, > thin tissues like insect antennae and insect GI tracts and midguts. > Since I get all my projects already fixed in whatever fixative the > investigator chooses, rinsed and placed in 70% ETOH the histogel never > touches formalin. I don't use formalin on my processor but start in > 70%. I've never had a problem with the histogel. We just put the > sample in the histogel flat and stand it up (turn 90?) when embedding > in paraffin. I use tissue prep for embedding. > If you don't want to use histogel you could try to put the drg's on GN > Metricel membrane disc filters. We do this with a lot of the samples I > receive, actually I have the investigators or their techs do this. The > tissue sticks to the membrane and orientation is a dream. The membrane > presents no problem when sectioning. You can get it from VWR. > > Andi > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cell Biology and Anatomy > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > "happy slicing and dicing and may all your stains work perfectly" - > Paula Sicurello > P Please consider the environment before printing this email. > > > > > On Feb 18, 2010, at 11:23 AM, Barone, Carol wrote: > > > Histonetter's...we received a "boat-load" of mouse DRGs that had been > > prepared in histogel and are cutting...well..not so good. > > We normally do DRGs from FS and get beautiful results. > > > > We have used histogel before with other small sample and have never > > had > > issues... not sure if it is the Histogel or DRG's (fixed in 10% NBF > > and > > then transferred to 70% before placed into the histogel).is the > > issue..I > > seem to remember that histogel requires formalin and wonder if the > > transfer to 70% is causing our problem ...but, obviously there is not > > much room for error with such tiny- tiny samples and they are already > > process and in paraffin? > > > > I am not quite sure how twe can improve the ones that came in > > histogel, > > and were processed to paraffin a paraffin block....any idea's? any > > experience? any anything? Thx- ASAP! > > > > cbarone@nemours.org > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/ From jkiernan <@t> uwo.ca Tue Feb 23 12:53:51 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Feb 23 12:53:55 2010 Subject: Fwd: Re: [Histonet] Bone tissue Message-ID: Just for the record, Mollifex isn't alkaline. It is probably either Baker's 1941 mixture (see below) or the following (parts by volume): 95% ethanol 952 Glycerol 32 Acetone 80 "Liquid phenol" 8 - - - "Liquid phenol" is probably liquefied phenol, USP, which contains about 10% water and at least 89% phenol (w/w), or something similar. For more information and discussion, see Maria Wynnchuk (1992) Minimizing artifacts in tissue processing: Part 1. Importance of softening agents. J. Histotechnol. 15(4): 321-323. This paper also discusses effects of softening faced blocks on H&E staining. John R Baker (1941) used 9 parts 60% ethanol and 1 part glycerol (J. Roy. Microsc. Soc. 61: 75), whereas R. C. Pearlman and Buell C. Cole (1951) sang the praises of 1% dishwashing detergent and similar solutions (Stain Technol. 26(2): 115-118). Manfred Gabe (Histological Techniques, Paris, 1976, a great book) favoured soaking faced paraffin blocks in cold water. For what it's worth, I have found water helpful when sectioning decalcified rats' heads. With all these well documented and inexpensive softening liquids it should never be necessary to resort to a proprietary product whose composition is kept secret from the buyer and user. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- > From: Rene J Buesa > Date: Monday, February 22, 2010 16:43 > Subject: Re: [Histonet] Bone tissue > To: histonet@lists.utsouthwestern.edu, Reuel Cornelia > > > First of all, Mollifex or any other alkaline substance will do > > nothing "useful" to the bone. > > I tend to think that you processed the bone before it was > > completely decalcified and that is the cause for an incomplete > > infiltration and a subsequent difficult sectioning. > > Ren? J. > > > > --- On Mon, 2/22/10, Reuel Cornelia > > wrote: > > > > From: Reuel Cornelia > > Subject: [Histonet] Bone tissue > > To: histonet@lists.utsouthwestern.edu > > Date: Monday, February 22, 2010, 4:36 PM > > > > > > We have a difficulty cutting metatarsal bone . It seems that our > > sections are so dried up. I was thinking that our dehydration > > have something to do with this which we have placed it in a > > wrong processing procedure for our large bone. The tissue is 4 > > mm thick and 1-2 cm in length and width and was dehydrated in > > 70% - 4 hrs, 80%-4 hrs,95% -4 hrs and 2 changes of 100% 3 hrs > > each, paraffin is 4 hrs each 2 changes. The tissue was > > decalcified in 14% EDTA. When we start cutting them it is so > > brittle and we could not even create a section. I have surfaced > > decal it and also place in a softener Mollifex some of it work > > but some does not work. Please help us remedy this tissue. Thank you. > > > > > > > > > > Reuel Cornelia, BS MT, AMT > > Cellular Pathology > > Texas Scottish Rite Hospital for Children > > 2222 Welborn Street > > Dallas, TX 75219 > > Tel: 214-559-7766 > > fax: 214-559-7768 From andreahooper <@t> rocketmail.com Tue Feb 23 13:06:23 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Tue Feb 23 13:06:28 2010 Subject: [Histonet] Double labeling with antibodies that need different fixatives In-Reply-To: <83FCA9E681495540A80ECBBAEF28B074F32A3C@cooper.meddent.uwa.edu.au> Message-ID: <456951.88103.qm@web113108.mail.gq1.yahoo.com> Hi Phebe, ? In my experience that CD31 clone you refer to doesn't work well mainly b/c of paraffin embedding - ?in combination with PFA/formalin fixation. On PFA/formalin fixed frozen samples it works just fine with a trypsin antigen retrieval step. I find it's the paraffin that is the real killer. Try Biocare's anti-CD31, that works on FFPE. Or use another marker of endothelium (if that's what you are using CD31 for). ? Alternatively, are you sure your second antibody only works in FFPE formalin fixed tissue? Because it may well work on formalin fixed frozen tissue, then you can use both on that instead (just remember to do the trypsin digestion). Or use fresh snap frozen tissue postfixed in PFA for 10 minutes then no antigen retrieval necessary. ? Good luck! Andrea Hooper --- Phebe Verbrugghe wrote: ??????? Hi all, ??? ??? ??? ??? I would like to do an immunofluorescent double labeling with two ??? ??? antibodies but 1 antibody works on acetone fixed frozen tissue but not ??? ??? on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) ??? ??? and the other one works on formalin fixed paraffin embedded tissue but ??? ??? not on acetone fixed frozen tissue. Is there any way I could still do a ??? ??? double labeling and how? ??? ??? Also, does anyone have experience with zinc fixative? If my antibody ??? ??? works on formalin fixed tissue is it likely to also work on zinc fixed ??? ??? tissue? ??? ??? ??? ??? Thank you very much in advance, ??? ??? ??? ??? Phebe ??????? From SohrabB1 <@t> ah.org Tue Feb 23 14:23:01 2010 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Tue Feb 23 14:23:21 2010 Subject: [Histonet] QA Report Message-ID: <4B83C8A5.4347.0054.0@ah.org> I was wondering if any one has special form that they provide to QA committee meeting in their hospital? This separate from all qc done in the lab!. Thanks,behnaz From leahcox27 <@t> yahoo.com Tue Feb 23 14:32:52 2010 From: leahcox27 <@t> yahoo.com (Leah Cox) Date: Tue Feb 23 14:32:56 2010 Subject: [Histonet] Any travel positions available? Message-ID: <474412.76472.qm@web112608.mail.gq1.yahoo.com> Has anyone heard of any travel or contract positions? From anonwums1 <@t> gmail.com Tue Feb 23 15:57:44 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Tue Feb 23 15:57:49 2010 Subject: [Histonet] Double labeling with antibodies that need different fixatives In-Reply-To: <456951.88103.qm@web113108.mail.gq1.yahoo.com> References: <83FCA9E681495540A80ECBBAEF28B074F32A3C@cooper.meddent.uwa.edu.au> <456951.88103.qm@web113108.mail.gq1.yahoo.com> Message-ID: <858249121002231357r649b6d2s575c43bdee3eb3c0@mail.gmail.com> On a related topic, does anyone know if the Biocare rat CD31 clone works for immunofluorescence on FFPE sections using a directly conjugated secondary antibody? Thanks, Adam On Tue, Feb 23, 2010 at 1:06 PM, Andrea T. Hooper < andreahooper@rocketmail.com> wrote: > Hi Phebe, > > In my experience that CD31 clone you refer to doesn't work well mainly b/c > of paraffin embedding - in combination with PFA/formalin fixation. On > PFA/formalin fixed frozen samples it works just fine with a trypsin antigen > retrieval step. I find it's the paraffin that is the real killer. Try > Biocare's anti-CD31, that works on FFPE. Or use another marker of > endothelium (if that's what you are using CD31 for). > > Alternatively, are you sure your second antibody only works in FFPE > formalin fixed tissue? Because it may well work on formalin fixed frozen > tissue, then you can use both on that instead (just remember to do the > trypsin digestion). Or use fresh snap frozen tissue postfixed in PFA for 10 > minutes then no antigen retrieval necessary. > > Good luck! > Andrea Hooper > > > --- Phebe Verbrugghe wrote: > > > Hi all, > > I would like to do an immunofluorescent double labeling > with two > antibodies but 1 antibody works on acetone fixed frozen > tissue but not > on formalin fixed paraffin embedded tissue (CD31 BD > pharmingen 553370) > and the other one works on formalin fixed paraffin > embedded tissue but > not on acetone fixed frozen tissue. Is there any way I > could still do a > double labeling and how? > Also, does anyone have experience with zinc fixative? If > my antibody > works on formalin fixed tissue is it likely to also work > on zinc fixed > tissue? > > Thank you very much in advance, > > Phebe > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From napoli <@t> siscom.net Tue Feb 23 18:01:09 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Tue Feb 23 18:01:13 2010 Subject: [Histonet] Leica Knife Holder CM1510 Message-ID: <4b846c45.152.509a.1294878881@siscom.net> If anyone is in need of a CM 1510 leica cryostat knife holder for low profile blades, please contact me. Willing to part with this still functional item at a reasonable cost. FOR LOW PROFILE BLADES. Good price for back-up or replacement for Mohs or other enterprise. Not an easy item to find used. From Kristopher.Kalleberg <@t> unilever.com Wed Feb 24 08:18:57 2010 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Wed Feb 24 08:19:06 2010 Subject: [Histonet] fontana Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C07C4C4E8@NTRSEVS30002.s3.ms.unilever.com> Does anyone know of anything other than melanin that Fontana Masson would stain? Also, does anyone know of a stain that would label un/saturated fatty acids? Not sure if this is even possible. Thanks in advance. Kris Kalleberg From CIngles <@t> uwhealth.org Wed Feb 24 10:36:02 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Feb 24 10:37:51 2010 Subject: [Histonet] NSH teleconference Message-ID: Would the person responsible for sending out NSH teleconference material please contact me. I have not recieved the materials for today. Claire Ingles UW Hospital and Clinics Madison WI From leahcox27 <@t> yahoo.com Wed Feb 24 10:41:53 2010 From: leahcox27 <@t> yahoo.com (Leah Cox) Date: Wed Feb 24 10:41:56 2010 Subject: [Histonet] Travel positions? Message-ID: <790956.75882.qm@web112613.mail.gq1.yahoo.com> Does anyone know of any travel positions? I have traveled in the past for a couple of years and I have 7 years experience all together. I am willing to go anywhere. Thanks Leah Cox From Valerie.Hannen <@t> parrishmed.com Wed Feb 24 10:48:07 2010 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Wed Feb 24 10:48:11 2010 Subject: [Histonet] Bcl-2 Message-ID: <5680DA93771F0C48954CC8D38425E72401AB3500@ISMAIL.parrishmed.local> Hi everyone, I am hoping you all can help me solve a problem that I am having with my Bcl-2 stain. I can get it to stain Follicular Lymphoma tissues, but for the life of me can not get normal tonsil tissue to stain. I use a Retrieval solution with a pH of 8, use Avidin / Biotin solutions, incubate in the primary for 60 minutes. The tonsil tissues that I am testing are inhouse fixed and processed tissues. We use Zinc Formalin for our fixation. Now that I think of it, I am also having trouble getting a purchased control of Lymphoma tissue to stain. The kit that we are using is the Histostain + kit, and we do our Immuno's manually. What am I doing wrong or what am I not doing? Thanks in advance to any and all replies. Valerie Hannen, Histotechnologist Parrish Medical Center Titusville, Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From andreahooper <@t> rocketmail.com Wed Feb 24 10:54:06 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Wed Feb 24 10:54:11 2010 Subject: [Histonet] Double labeling with antibodies that need different fixatives In-Reply-To: <858249121002231357r649b6d2s575c43bdee3eb3c0@mail.gmail.com> Message-ID: <344783.60535.qm@web113108.mail.gq1.yahoo.com> I haven't tried it directly but I don't see why not. If it's an amplification problem you can always amplify the fluorescence. That being said I am really not a huge fan of IF on FFPE especially when it comes to vessels as many vessels have high autofluorescence not to mention the autofluorescence from RBCs. I am afraid of false positives. --- On Tue, 2/23/10, Adam . wrote: From: Adam . Subject: Re: [Histonet] Double labeling with antibodies that need different fixatives To: "Andrea T. Hooper" Cc: "Phebe Verbrugghe" , histonet@lists.utsouthwestern.edu Date: Tuesday, February 23, 2010, 9:57 PM On a related topic, does anyone know if the Biocare rat CD31 clone works for immunofluorescence on FFPE sections using a directly conjugated secondary antibody? Thanks, Adam On Tue, Feb 23, 2010 at 1:06 PM, Andrea T. Hooper wrote: Hi Phebe, ? In my experience that CD31 clone you refer to doesn't work well mainly b/c of paraffin embedding - ?in combination with PFA/formalin fixation. On PFA/formalin fixed frozen samples it works just fine with a trypsin antigen retrieval step. I find it's the paraffin that is the real killer. Try Biocare's anti-CD31, that works on FFPE. Or use another marker of endothelium (if that's what you are using CD31 for). ? Alternatively, are you sure your second antibody only works in FFPE formalin fixed tissue? Because it may well work on formalin fixed frozen tissue, then you can use both on that instead (just remember to do the trypsin digestion). Or use fresh snap frozen tissue postfixed in PFA for 10 minutes then no antigen retrieval necessary. ? Good luck! Andrea Hooper --- Phebe Verbrugghe wrote: ??????? Hi all, ??? ??? ??? ??? I would like to do an immunofluorescent double labeling with two ??? ??? antibodies but 1 antibody works on acetone fixed frozen tissue but not ??? ??? on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) ??? ??? and the other one works on formalin fixed paraffin embedded tissue but ??? ??? not on acetone fixed frozen tissue. Is there any way I could still do a ??? ??? double labeling and how? ??? ??? Also, does anyone have experience with zinc fixative? If my antibody ??? ??? works on formalin fixed tissue is it likely to also work on zinc fixed ??? ??? tissue? ??? ??? ??? ??? Thank you very much in advance, ??? ??? ??? ??? Phebe ??????? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Feb 24 10:59:45 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Feb 24 10:59:50 2010 Subject: [Histonet] Tris Buffer Preservative Message-ID: <582736991002240859l54e7d3f2if0eeed92f51e3f1f@mail.gmail.com> Hi, I have been making up my own tris buffer for immunohistochemistry. It has been working swimmingly, with one exception. I have been doing some work on Salmonella, and apparently the researcher has been seeing bacteria in her immunofluorescent slides that she says are moving and seem to be viable. She wanted to culture all my reagents. So the primary and secondary antibody diluents (commercially purchased) came up clean, but the TBS ended up with a pretty healthy growing colony. Now since I don't do IHC overnight in an incubator, I don't think this is necessarily a catastrophy. (No one else has noticed this) It does seem to warrant further investigation though. So for the folks that make their own solutions up, what do you use as a preservative for your buffers and how much do you use. I haven't seen anything in any of the recipes I have found. I was thinking Sodium Azide, but it is really hazardous, and Wikipedia says it is actually explosive ( http://en.wikipedia.org/wiki/Sodium_azide). Biocare Medical's data sheet says they use less than 0.25% procyclin. (thanks for not hiding the ingredients Biocare, I love you for that!) Has anyone tried that? Any other suggestions would be welcome. Thanks, Amos From cpyse <@t> x-celllab.com Wed Feb 24 11:05:26 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Feb 24 11:08:31 2010 Subject: [Histonet] fontana In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C07C4C4E8@NTRSEVS30002.s3.ms.unilever.com> References: <0E6BC087F70F9C47ACFF2C203D6E329C07C4C4E8@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <000001cab573$8f7b2800$ae717800$@com> Fontana-Masson also stains for Argentaffin granuals. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Wednesday, February 24, 2010 9:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fontana Does anyone know of anything other than melanin that Fontana Masson would stain? Also, does anyone know of a stain that would label un/saturated fatty acids? Not sure if this is even possible. Thanks in advance. Kris Kalleberg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 24 11:10:57 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 24 11:11:00 2010 Subject: [Histonet] fontana In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C07C4C4E8@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <321397.55585.qm@web65707.mail.ac4.yahoo.com> Any histological structure able to reduce silver into a black precipitate (argentaffin reaction) will be stained with the Fontana-Masson procedure. Ren? J. --- On Wed, 2/24/10, Kalleberg, Kristopher wrote: From: Kalleberg, Kristopher Subject: [Histonet] fontana To: histonet@lists.utsouthwestern.edu Date: Wednesday, February 24, 2010, 9:18 AM Does anyone know of anything other than melanin that Fontana Masson would stain?? Also, does anyone know of a stain that would label un/saturated fatty acids?? Not sure if this is even possible.? Thanks in advance. Kris Kalleberg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 24 11:14:37 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 24 11:14:41 2010 Subject: [Histonet] Bcl-2 In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB3500@ISMAIL.parrishmed.local> Message-ID: <419199.31608.qm@web65705.mail.ac4.yahoo.com> I always did HIER at pH6 for BCL-2, diluted? at 1:10 (DAKO Ab) during 30 min incubarion. FFPE tonsil as control. Ren? J. --- On Wed, 2/24/10, Hannen, Valerie wrote: From: Hannen, Valerie Subject: [Histonet] Bcl-2 To: histonet@lists.utsouthwestern.edu Date: Wednesday, February 24, 2010, 11:48 AM Hi everyone, I am hoping you all can help me solve a problem that I am having with my Bcl-2 stain. I can get it to stain Follicular Lymphoma tissues, but for the life of me can not get normal tonsil tissue to stain.? I use a Retrieval solution with a pH of 8, use Avidin / Biotin solutions, incubate in the primary for 60 minutes. The tonsil tissues that I am testing are inhouse fixed and processed tissues. We use Zinc Formalin for our fixation. Now that I think of it, I am also having trouble getting a purchased control of Lymphoma tissue to stain.? The kit that we are using is the Histostain + kit, and we do our Immuno's manually. What am I doing wrong or what am I not doing?? Thanks in advance to any and all replies. Valerie Hannen, Histotechnologist Parrish Medical Center Titusville, Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 24 11:16:33 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 24 11:16:38 2010 Subject: [Histonet] Tris Buffer Preservative In-Reply-To: <582736991002240859l54e7d3f2if0eeed92f51e3f1f@mail.gmail.com> Message-ID: <522269.95631.qm@web65712.mail.ac4.yahoo.com> I never used preservatives but I also never stored the buffer more than 5 days. Ren? J. --- On Wed, 2/24/10, Amos Brooks wrote: From: Amos Brooks Subject: [Histonet] Tris Buffer Preservative To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, February 24, 2010, 11:59 AM Hi, ???I have been making up my own tris buffer for immunohistochemistry. It has been working swimmingly, with one exception. I have been doing some work on Salmonella, and apparently the researcher has been seeing bacteria in her immunofluorescent slides that she says are moving and seem to be viable. She wanted to culture all my reagents. So the primary and secondary antibody diluents (commercially purchased) came up clean, but the TBS ended up with a pretty healthy growing colony. Now since I don't do IHC overnight in an incubator, I don't think this is necessarily a catastrophy. (No one else has noticed this) It does seem to warrant further investigation though. So for the folks that make their own solutions up, what do you use as a preservative for your buffers and how much do you use. I haven't seen anything in any of the recipes I have found. I was thinking Sodium Azide, but it is really hazardous, and Wikipedia says it is actually explosive ( http://en.wikipedia.org/wiki/Sodium_azide). Biocare Medical's data sheet says they use less than 0.25% procyclin. (thanks for not hiding the ingredients Biocare, I love you for that!) Has anyone tried that? Any other suggestions would be welcome. Thanks, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Wed Feb 24 11:22:33 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Feb 24 11:22:49 2010 Subject: [Histonet] Sirius Red In-Reply-To: <321397.55585.qm@web65707.mail.ac4.yahoo.com> References: <0E6BC087F70F9C47ACFF2C203D6E329C07C4C4E8@NTRSEVS30002.s3.ms.unilever.com> <321397.55585.qm@web65707.mail.ac4.yahoo.com> Message-ID: Is there a good Sirius Red procedure used to stain collagen And where can I find the powder _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From histonetalias <@t> gmail.com Wed Feb 24 11:32:02 2010 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Wed Feb 24 11:32:07 2010 Subject: [Histonet] Sirius Red In-Reply-To: References: <0E6BC087F70F9C47ACFF2C203D6E329C07C4C4E8@NTRSEVS30002.s3.ms.unilever.com> <321397.55585.qm@web65707.mail.ac4.yahoo.com> Message-ID: <4b6c85511002240932u2ef3b1b1mc748a0285c56385e@mail.gmail.com> Here is a procedure that I found in the archives. http://www.histosearch.com/histonet/Jul01A/Siriusredcollagenprocedur.html And here is another... Sirius Red Stain Picro-sirius red Sirius Red F3B (CI 35780) Aldrich 36,554-8 synonym Direct Red 80 0.5gm Saturated aqueous Picric Acid 500ml [add a small amount of solid picric acid to ensure saturation: 1.2~1.3% (W/V)] Expiration date minimum 3 years Acidified Water Acetic Acid (glacial) 5 ml Water (DI or tap) 1 liter ? Fix frozen sections 30 minutes in Neutral Buffered Formalin(For paraffin sections deparaffinize and bring slides to water) ? Wash sections in 3 changes of water ? Drain sections well and place into Picro-Sirius solution 2 min ? Wash sections in 2 changes of acidified water(5min) ? Dehydrate in 3 changes of 100% ethanol (5min) ? Clear in xylene and mount in permanent mounting media (5min).b I found the powder here.... http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=43665|FLUKA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC On Wed, Feb 24, 2010 at 12:22 PM, Nails, Felton wrote: > Is there a good Sirius Red procedure used to stain collagen > And where can I find the powder > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ============================================================ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From jkiernan <@t> uwo.ca Wed Feb 24 11:35:56 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Feb 24 11:36:03 2010 Subject: [Histonet] Sirius Red In-Reply-To: References: <0E6BC087F70F9C47ACFF2C203D6E329C07C4C4E8@NTRSEVS30002.s3.ms.unilever.com> <321397.55585.qm@web65707.mail.ac4.yahoo.com> Message-ID: You'll find brief instructions for the picro-sirius red method for collagen in nearly every histological techniques book published since about 1970. A Google search for "sirius red vendor" brings up 484 hits, though not all are for vendors. It's important to get the correct dye (C.I. 35780, Direct red 80). For a thorough discussion of how to use the method intelligently, see L. Junqueira et al 1979. Histochem. J. 11: 447-455. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Nails, Felton" Date: Wednesday, February 24, 2010 12:23 Subject: [Histonet] Sirius Red To: "histonet@lists.utsouthwestern.edu" > Is there a good Sirius Red procedure used to stain collagen > And where can I find the powder > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ============================================================ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Wed Feb 24 11:55:52 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Feb 24 11:56:16 2010 Subject: [Histonet] Sirius Red In-Reply-To: References: <0E6BC087F70F9C47ACFF2C203D6E329C07C4C4E8@NTRSEVS30002.s3.ms.unilever.com> <321397.55585.qm@web65707.mail.ac4.yahoo.com> Message-ID: Thanks for everyone's help ________________________________ From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: Wednesday, February 24, 2010 11:36 AM To: Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Sirius Red You'll find brief instructions for the picro-sirius red method for collagen in nearly every histological techniques book published since about 1970. A Google search for "sirius red vendor" brings up 484 hits, though not all are for vendors. It's important to get the correct dye (C.I. 35780, Direct red 80). For a thorough discussion of how to use the method intelligently, see L. Junqueira et al 1979. Histochem. J. 11: 447-455. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Nails, Felton" Date: Wednesday, February 24, 2010 12:23 Subject: [Histonet] Sirius Red To: "histonet@lists.utsouthwestern.edu" > Is there a good Sirius Red procedure used to stain collagen > And where can I find the powder > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ============================================================ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From algranth <@t> email.arizona.edu Wed Feb 24 11:59:20 2010 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Wed Feb 24 11:59:26 2010 Subject: [Histonet] Sirius Red In-Reply-To: References: <0E6BC087F70F9C47ACFF2C203D6E329C07C4C4E8@NTRSEVS30002.s3.ms.unilever.com> <321397.55585.qm@web65707.mail.ac4.yahoo.com> Message-ID: <3F3E5D6A-61CD-4486-B1B7-3EB6B7113F7F@email.arizona.edu> I use the Puchtler's Picro-Sirius Red Stain that I got from Stains File online. This stain works beautifully and the collagen is really pretty when using polorized light microscopy. The Sirius Red that I use was here in the lab so I didn't have to buy it but it is Sirius Red F3B. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Feb 24, 2010, at 10:22 AM, Nails, Felton wrote: > Is there a good Sirius Red procedure used to stain collagen > And where can I find the powder > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ============================================================ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Wed Feb 24 12:05:22 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Feb 24 12:05:34 2010 Subject: AW: [Histonet] Bcl-2 In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB3500@ISMAIL.parrishmed.local> References: <5680DA93771F0C48954CC8D38425E72401AB3500@ISMAIL.parrishmed.local> Message-ID: Valerie, I would look at the fixation time of the tonsils and on their storage time. If the tonsils are fixed much longer than your lymphoma specimens a harsher pretreatment is necessary. If the control-tonsils are stored for long time the surface could suffer from oxidation. Do you have fresh tonsil for testing? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Hannen, Valerie Gesendet: Mittwoch, 24. Februar 2010 17:48 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bcl-2 Hi everyone, I am hoping you all can help me solve a problem that I am having with my Bcl-2 stain. I can get it to stain Follicular Lymphoma tissues, but for the life of me can not get normal tonsil tissue to stain. I use a Retrieval solution with a pH of 8, use Avidin / Biotin solutions, incubate in the primary for 60 minutes. The tonsil tissues that I am testing are inhouse fixed and processed tissues. We use Zinc Formalin for our fixation. Now that I think of it, I am also having trouble getting a purchased control of Lymphoma tissue to stain. The kit that we are using is the Histostain + kit, and we do our Immuno's manually. What am I doing wrong or what am I not doing? Thanks in advance to any and all replies. Valerie Hannen, Histotechnologist Parrish Medical Center Titusville, Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Feb 24 12:07:35 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Feb 24 12:07:55 2010 Subject: AW: [Histonet] fontana In-Reply-To: <000001cab573$8f7b2800$ae717800$@com> References: <0E6BC087F70F9C47ACFF2C203D6E329C07C4C4E8@NTRSEVS30002.s3.ms.unilever.com> <000001cab573$8f7b2800$ae717800$@com> Message-ID: Kris, Formalin pigment would give false positive staining with Fontana Masson. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cynthia Pyse Gesendet: Mittwoch, 24. Februar 2010 18:05 An: 'Kalleberg, Kristopher'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] fontana Fontana-Masson also stains for Argentaffin granuals. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Wednesday, February 24, 2010 9:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fontana Does anyone know of anything other than melanin that Fontana Masson would stain? Also, does anyone know of a stain that would label un/saturated fatty acids? Not sure if this is even possible. Thanks in advance. Kris Kalleberg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Feb 24 12:11:58 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Feb 24 12:12:02 2010 Subject: [Histonet] 1. Fontana. 2. Fatty acids Message-ID: 1. The list of argentaffin materials includes melanin, granules of some neuroendocrine cells (including chromaffin cells, carcinoid tumours etc), lipofuscin and formalin pigment. There was a special issue of the Journal of Histolotechnology devoted to silver staining (Vol 19, No, 3, Sept 1996). Detailed instructions for two methods for melanin are given on pp.274-275 in a paper by Winsome Garvey. 2. Almost all staining methods for fat and other lipids depend on the presence of unsaturated acyl groups. For free fatty acids (i.e. not esterified) there are methods using Nile blue (CI 51180) and there's also Holczinger's method using copper acetate and dithiooxamide. Every histochemistry book gives instructions for these methods and discusses their limitations. Pearse (4th ed Vol 2) is the most thorough, but there's an excellent little 68-page paperback, "Lipid Histochemistry" by Olga Bayliss High (Oxford Univ Press and Royal Microscopical Society, 1984). It's probably out of print now, but a Google search for "ISBN 0198564058" brings up plenty of 2nd-hand copies. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Kalleberg, Kristopher" Date: Wednesday, February 24, 2010 9:20 Subject: [Histonet] fontana To: histonet@lists.utsouthwestern.edu > Does anyone know of anything other than melanin that Fontana Masson > would stain? Also, does anyone know of a stain that would label > un/saturated fatty acids? Not sure if this is even > possible. Thanks in > advance. > > Kris Kalleberg > _______________________________________________ From Allison_Scott <@t> hchd.tmc.edu Wed Feb 24 12:24:33 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Feb 24 12:24:39 2010 Subject: [Histonet] Paraffin Accumulation on floor Message-ID: <1872B4A455B7974391609AD8034C79FC8BD6CD@LBEXCH01.hchd.local> How is everyone dealing with the accumulation and tracking of paraffin problems. We have mats on the floor where we cut and embed. We also have the mats that you walk on and it takes the paraffin off of the bottom of your shoes and you peel off a layer a day. Our medical director wants us to find a way to keep the floors clear, so that no one will slip and fall. I have never seen a histololgy lab that did not have a paraffin tracking problem. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From ndevans <@t> stanford.edu Wed Feb 24 12:33:57 2010 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Wed Feb 24 12:34:04 2010 Subject: [Histonet] M.O.M frozen section background Message-ID: <1424461716.84681267036437219.JavaMail.root@zm06.stanford.edu> Hi All, Does anyone have any tips on reducing background staining when attempting to detect mouse monoclonal antibodies on mouse tissue? We currently use Vector's M.O.M kit - it works great on PPFE tissue with nice specific staining after pepsin unmasking, but in frozen tissue the background is much higher than the signal. I routinely use 4% PFA pre-fix, then 3% H2O2 in MeOH for 3 mins, then all the blocks that come with teh kit. I am using several different cytokeratin Abs on 10 um frozen mouse sections. The only thing that I could think to try was to dehydrate the tissues, rehydrate and try again with the unmasking, but if anyone has a better idea or commonly deals with this problem, please let me know. Best wishes Nick From jqb7 <@t> cdc.gov Wed Feb 24 12:34:42 2010 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Feb 24 12:34:53 2010 Subject: [Histonet] Paraffin Accumulation on floor In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD6CD@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC8BD6CD@LBEXCH01.hchd.local> Message-ID: <68510B12184E45498EABD4CB6F3868FE1EF36F@LTA3VS001.ees.hhs.gov> We have a long-handled scraper and use it periodically. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, February 24, 2010 1:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Accumulation on floor How is everyone dealing with the accumulation and tracking of paraffin problems. We have mats on the floor where we cut and embed. We also have the mats that you walk on and it takes the paraffin off of the bottom of your shoes and you peel off a layer a day. Our medical director wants us to find a way to keep the floors clear, so that no one will slip and fall. I have never seen a histololgy lab that did not have a paraffin tracking problem. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Feb 24 12:39:51 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Feb 24 12:39:56 2010 Subject: [Histonet] Paraffin Accumulation on floor In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD6CD@LBEXCH01.hchd.local> Message-ID: We also use the long-handled scraper. Our greatest problem though, was the hallway outside of Histology where non-technical staff would be walking in heels. We eventually had that hall carpeted, which has been a great improvement. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scott, Allison D Sent: Wednesday, February 24, 2010 1:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Accumulation on floor How is everyone dealing with the accumulation and tracking of paraffin problems. We have mats on the floor where we cut and embed. We also have the mats that you walk on and it takes the paraffin off of the bottom of your shoes and you peel off a layer a day. Our medical director wants us to find a way to keep the floors clear, so that no one will slip and fall. I have never seen a histololgy lab that did not have a paraffin tracking problem. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From tpodawiltz <@t> lrgh.org Wed Feb 24 12:39:32 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Feb 24 12:44:13 2010 Subject: [Histonet] Paraffin Accumulation on floor In-Reply-To: <68510B12184E45498EABD4CB6F3868FE1EF36F@LTA3VS001.ees.hhs.gov> References: <1872B4A455B7974391609AD8034C79FC8BD6CD@LBEXCH01.hchd.local>, <68510B12184E45498EABD4CB6F3868FE1EF36F@LTA3VS001.ees.hhs.gov> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5B1FACD@LRGHEXVS1.practice.lrgh.org> We just bought everyone skates. Seriously, we have a scraper that we use, along with the various mats. What we just did was lay the traction strips on the floor outside of the Histology lab. That was the area were most people, myself included have slipped and fallen. Ironically, we found no paraffin on the floor. Which is why the traction strips were but on the floor. The other observation that we made was that most of the people that slipped were wearing dress shoes, not idustrial/nursing style foot wear. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) [jqb7@cdc.gov] Sent: Wednesday, February 24, 2010 1:34 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Accumulation on floor We have a long-handled scraper and use it periodically. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, February 24, 2010 1:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Accumulation on floor How is everyone dealing with the accumulation and tracking of paraffin problems. We have mats on the floor where we cut and embed. We also have the mats that you walk on and it takes the paraffin off of the bottom of your shoes and you peel off a layer a day. Our medical director wants us to find a way to keep the floors clear, so that no one will slip and fall. I have never seen a histololgy lab that did not have a paraffin tracking problem. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From srishan <@t> mail.holyname.org Wed Feb 24 13:22:46 2010 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Wed Feb 24 13:23:20 2010 Subject: [Histonet] disinfectant for ventana xt decon process Message-ID: Hi All, I am trying to get AMPHYL disinfectant for the decon procedure for ventana XT but heard that the product is discontnued by the manufacturers. If anyone is using a substitute for this, please give me the vendor and the part number. Thanks Nirmala Srishan Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare , 2009 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From collette2 <@t> mail.llnl.gov Wed Feb 24 13:24:15 2010 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Wed Feb 24 13:23:46 2010 Subject: [Histonet] Tris Buffer Preservative In-Reply-To: <582736991002240859l54e7d3f2if0eeed92f51e3f1f@mail.gmail.com> References: <582736991002240859l54e7d3f2if0eeed92f51e3f1f@mail.gmail.com> Message-ID: Hello, Amos, It is pretty straightforward to make fresh, why would you want to store it? We use sterile 1M Tris, pH 7.4, and 4M NaCl as stocks, they are concentrated enough that nothing obvious grows in them, dilute the volume you need, pH if needed, (add the Tween if needed), and store in the fridge for a single experiment. Alternatively, you can make a sterile 10X salt stock, dilute that as needed to make the math easier. Throw out the leftover diluted solution so nobody else tries to use it. In my experience, TBS(T) will ALWAYS get cloudy when left to sit, I wouldn't risk the contamination. However, I'm not in a high-throughput lab, so this works for me (I'm a one-pair-of-hands histology lab). Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley At 11:59 AM -0500 2/24/10, Amos Brooks wrote: >Hi, > I have been making up my own tris buffer for immunohistochemistry. It has >been working swimmingly, with one exception. I have been doing some work on >Salmonella, and apparently the researcher has been seeing bacteria in her >immunofluorescent slides that she says are moving and seem to be viable. She >wanted to culture all my reagents. So the primary and secondary antibody >diluents (commercially purchased) came up clean, but the TBS ended up with a >pretty healthy growing colony. Now since I don't do IHC overnight in an >incubator, I don't think this is necessarily a catastrophy. (No one else has >noticed this) It does seem to warrant further investigation though. So for >the folks that make their own solutions up, what do you use as a >preservative for your buffers and how much do you use. I haven't seen >anything in any of the recipes I have found. I was thinking Sodium Azide, >but it is really hazardous, and Wikipedia says it is actually explosive ( >http://*en.wikipedia.org/wiki/Sodium_azide). Biocare Medical's data sheet >says they use less than 0.25% procyclin. (thanks for not hiding the >ingredients Biocare, I love you for that!) Has anyone tried that? Any other >suggestions would be welcome. > >Thanks, >Amos >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://*lists.utsouthwestern.edu/mailman/listinfo/histonet From dcojita <@t> tampabay.rr.com Wed Feb 24 13:42:42 2010 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Wed Feb 24 13:42:46 2010 Subject: [Histonet] FISH PROBES Message-ID: Does anyone know of a reliable company to purchase FISH probes from (other than Abbott)? From leiker <@t> buffalo.edu Wed Feb 24 14:08:23 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Feb 24 14:08:30 2010 Subject: [Histonet] FISH PROBES In-Reply-To: References: Message-ID: IDLabs works great for us. Cambio is referenced a lot in the literature, but their Y-chromosome porcine probes didn't work for us. --On Wednesday, February 24, 2010 2:42 PM -0500 dcojita@tampabay.rr.com wrote: > Does anyone know of a reliable company to purchase FISH probes from (other > than Abbott)? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From andreahooper <@t> rocketmail.com Wed Feb 24 14:52:52 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Wed Feb 24 14:52:57 2010 Subject: [Histonet] M.O.M frozen section background In-Reply-To: <1424461716.84681267036437219.JavaMail.root@zm06.stanford.edu> Message-ID: <56189.99037.qm@web113108.mail.gq1.yahoo.com> What types of mouse tissue are you working with? Are they fresh frozen with post-fixation or fixed frozen? Do you block biotin? Do you have an isotype control and?a no primary control?? I am thinking biotin's the likely culprit as the kit doesn't come with the very necessary biotin blocking reagents ... ? Andrea Hooper --- On Wed, 2/24/10, Nicholas David Evans wrote: From: Nicholas David Evans Subject: [Histonet] M.O.M frozen section background To: "histonet" Date: Wednesday, February 24, 2010, 6:33 PM Hi All, Does anyone have any tips on reducing background staining when attempting to detect mouse monoclonal antibodies on mouse tissue? We currently use Vector's M.O.M kit? - it works great on PPFE tissue with nice specific staining after pepsin unmasking, but in frozen tissue the background is much higher than the signal. I routinely use 4% PFA pre-fix, then 3% H2O2 in MeOH for 3 mins, then all the blocks that come with teh kit. I am using several different cytokeratin Abs on 10 um frozen mouse sections. The only thing that I could think to try was to dehydrate the tissues, rehydrate and try again with the unmasking, but if anyone has a better idea or commonly deals with this problem, please let me know. Best wishes Nick _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Wed Feb 24 14:55:30 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Wed Feb 24 14:56:13 2010 Subject: [Histonet] Double labeling with antibodies that need differentfixatives In-Reply-To: <201002231109101359724@foxmail.com> Message-ID: <538881.34148.qm@web113110.mail.gq1.yahoo.com> Not sure if you were asking me or not and it's a broad answer but VE-cadherin is a good one - the best overall marker of endothelium. vWF is another one that works well for most tumors. If staining mouse organs, if you let me know what tissue you are staining I can recommend the best specific marker of that tissue's vasculature. ? Andrea Hooper --- On Tue, 2/23/10, TF wrote: From: TF Subject: Re: Re: [Histonet] Double labeling with antibodies that need differentfixatives To: "Adam ." , "Phebe Verbrugghe" Cc: "histonet" Date: Tuesday, February 23, 2010, 3:09 AM what is the other marker than CD31? May you let us know? 2010-02-23 TF From GenieJacobs <@t> texashealth.org Wed Feb 24 15:12:14 2010 From: GenieJacobs <@t> texashealth.org (Jacobs, Genie) Date: Wed Feb 24 15:12:27 2010 Subject: [Histonet] job Message-ID: <7D894CD1537BE943BBF97DEDAFD8B4F454787FDC@PHDEXMB03.txhealth.org> Looking for part-time histotech Private lab in Plano Tx. 972 981 3108 The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From laurie.colbert <@t> huntingtonhospital.com Wed Feb 24 15:54:53 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Feb 24 15:54:58 2010 Subject: [Histonet] Snap Freezing Tissue Message-ID: <57BE698966D5C54EAE8612E8941D7683080979D6@EXCHANGE3.huntingtonhospital.com> I was wondering how others are snap freezing tissue? We have been using a histobath (a refrigerator device that cools isopentane for freezing tissue), but it has died. I would love to have another Histobath, but they are no longer carried by the vendor that I originally bought mine from. Does anyone know where I can get either a new or refurbished Histobath (vendor calls are welcome)? I'm also interested in other freezing techniques. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 From andreahooper <@t> rocketmail.com Wed Feb 24 16:13:41 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Wed Feb 24 16:13:46 2010 Subject: [Histonet] Snap Freezing Tissue In-Reply-To: <57BE698966D5C54EAE8612E8941D7683080979D6@EXCHANGE3.huntingtonhospital.com> Message-ID: <91441.76477.qm@web113105.mail.gq1.yahoo.com> I have used the "Gayle Callis" method of freezing tissue for histological applications for years with great success :)?The tissues look fantastic. ? Essentially float out a metal pan (like the ones you sterilize surgical tools in) on a liquid nitrogen bath (in a styrofoam container or large dewar). Place fresh or cryoprotected tissue into cryomold (I like the Tissue Tek ones mainly) and add sufficient OCT. Place mold onto metal tray and close lid of dewar or styrofoam container. Let freeze. When frozen wrap in foil and place into bitran bag, place into -80 deg C for long-term storage. ? If snap freezing tissue for RNA/protein?extraction I use the classic isopentane cooling method. ? Andrea T. Hooper --- On Wed, 2/24/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Snap Freezing Tissue To: histonet@lists.utsouthwestern.edu Cc: "Steve Pike" Date: Wednesday, February 24, 2010, 9:54 PM I was wondering how others are snap freezing tissue?? We have been using a histobath (a refrigerator device that cools isopentane for freezing tissue), but it has died.? I would love to have another Histobath, but they are no longer carried by the vendor that I originally bought mine from.? Does anyone know where I can get either a new or refurbished Histobath (vendor calls are welcome)?? I'm also interested in other freezing techniques. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anonwums1 <@t> gmail.com Wed Feb 24 16:31:58 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Wed Feb 24 16:32:04 2010 Subject: [Histonet] Snap Freezing Tissue In-Reply-To: <91441.76477.qm@web113105.mail.gq1.yahoo.com> References: <57BE698966D5C54EAE8612E8941D7683080979D6@EXCHANGE3.huntingtonhospital.com> <91441.76477.qm@web113105.mail.gq1.yahoo.com> Message-ID: <858249121002241431g1452b8fbr1c665e30f8ec2d08@mail.gmail.com> I learned to freeze tissue by taking a beaker and filling it with 2-methylbutane and then placing that beaker in a liquid nitrogen bath. You then take place your cryomold, hold it using some long forceps, and place it on the top of the 2-methylbutane. It will freeze the entire thing in about 10 seconds. Once it's frozen, you dip the entire mold into the 2-methylbutane. Seems to work well for me. Adam On Wed, Feb 24, 2010 at 4:13 PM, Andrea T. Hooper < andreahooper@rocketmail.com> wrote: > I have used the "Gayle Callis" method of freezing tissue for histological > applications for years with great success :) The tissues look fantastic. > > Essentially float out a metal pan (like the ones you sterilize surgical > tools in) on a liquid nitrogen bath (in a styrofoam container or large > dewar). Place fresh or cryoprotected tissue into cryomold (I like the Tissue > Tek ones mainly) and add sufficient OCT. Place mold onto metal tray and > close lid of dewar or styrofoam container. Let freeze. When frozen wrap in > foil and place into bitran bag, place into -80 deg C for long-term storage. > > If snap freezing tissue for RNA/protein extraction I use the classic > isopentane cooling method. > > Andrea T. Hooper > > > --- On Wed, 2/24/10, Laurie Colbert > wrote: > > > From: Laurie Colbert > Subject: [Histonet] Snap Freezing Tissue > To: histonet@lists.utsouthwestern.edu > Cc: "Steve Pike" > Date: Wednesday, February 24, 2010, 9:54 PM > > > I was wondering how others are snap freezing tissue? We have been using > a histobath (a refrigerator device that cools isopentane for freezing > tissue), but it has died. I would love to have another Histobath, but > they are no longer carried by the vendor that I originally bought mine > from. Does anyone know where I can get either a new or refurbished > Histobath (vendor calls are welcome)? I'm also interested in other > freezing techniques. > > > > Laurie Colbert > > Huntington Hospital > > Pasadena, CA > > (626) 397-8620 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Traczyk7 <@t> aol.com Wed Feb 24 18:57:02 2010 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Wed Feb 24 18:57:29 2010 Subject: [Histonet] Snap Freezing Tissue Message-ID: <3dad8.4ff1940c.38b724de@aol.com> Contact Jim Mullen at Hacker Instruments (800) 442-2537. He has a demo CliniRF that I'm sure you can get at a good price. Dorothy Dorothy Traczyk MTA Histology LLC In a message dated 2/24/2010 4:55:55 P.M. Eastern Standard Time, laurie.colbert@huntingtonhospital.com writes: I was wondering how others are snap freezing tissue? We have been using a histobath (a refrigerator device that cools isopentane for freezing tissue), but it has died. I would love to have another Histobath, but they are no longer carried by the vendor that I originally bought mine from. Does anyone know where I can get either a new or refurbished Histobath (vendor calls are welcome)? I'm also interested in other freezing techniques. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ndevans <@t> stanford.edu Wed Feb 24 19:00:48 2010 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Wed Feb 24 19:00:55 2010 Subject: [Histonet] M.O.M frozen section background In-Reply-To: References: Message-ID: <9F4D22CDA7A44D7FB753895AC18FDDB8@DellDesktop2> You made me worried there for a minute, Charles - I've spent a lot of time cryosectioning rather than using PPFE! But luckily dehydrating, rehydrating and post-fix seemed to completely get rid of the background I was experiencing. No idea why it works, but it did. (We cryoprotect In sucrose, then embed in methanol in dry ice). Best wishes Nick _____ From: Charles.Scouten@leica-microsystems.com [mailto:Charles.Scouten@leica-microsystems.com] Sent: Wednesday, February 24, 2010 2:03 PM To: ndevans@stanford.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] M.O.M frozen section background You must be using a stain for a protein or molecule present in cytosol. The frozen sections are different that the paraffin because the freezing process was too slow. Ice crystals formed, and broke membranes. Cytosol leaked into the intracellular space, and stained there. Background. Snap freeze in less than 3 seconds to solid, and the crystals cannot form. Full Immersion in isopentane (flammable) mixed into a slurry with dry ice would do it. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas David Evans Sent: Wednesday, February 24, 2010 12:34 PM To: histonet Subject: [Histonet] M.O.M frozen section background Hi All, Does anyone have any tips on reducing background staining when attempting to detect mouse monoclonal antibodies on mouse tissue? We currently use Vector's M.O.M kit - it works great on PPFE tissue with nice specific staining after pepsin unmasking, but in frozen tissue the background is much higher than the signal. I routinely use 4% PFA pre-fix, then 3% H2O2 in MeOH for 3 mins, then all the blocks that come with teh kit. I am using several different cytokeratin Abs on 10 um frozen mouse sections. The only thing that I could think to try was to dehydrate the tissues, rehydrate and try again with the unmasking, but if anyone has a better idea or commonly deals with this problem, please let me know. Best wishes Nick _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From vhlwong <@t> yahoo.com Wed Feb 24 19:34:06 2010 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Wed Feb 24 19:34:09 2010 Subject: [Histonet] Decalcification and processing of large bone Message-ID: <347173.34928.qm@web52706.mail.re2.yahoo.com> Dear all, ? I am going to decal and process?PIG femur and lumbar vertebrates.? I only have experience on soft tissues.? Anyone can suggest any protocols in decalcification and processing?? Many thanks in advance. ? Cheers, Victor From jaylundgren <@t> gmail.com Thu Feb 25 01:32:12 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Feb 25 01:32:20 2010 Subject: [Histonet] Paraffin Accumulation on floor In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323D5B1FACD@LRGHEXVS1.practice.lrgh.org> References: <1872B4A455B7974391609AD8034C79FC8BD6CD@LBEXCH01.hchd.local> <68510B12184E45498EABD4CB6F3868FE1EF36F@LTA3VS001.ees.hhs.gov> <38667E7FB77ECD4E91BFAEB8D986386323D5B1FACD@LRGHEXVS1.practice.lrgh.org> Message-ID: I have noticed that most slips happen in the junctions between the paraffin covered floors and " uncoated" floors, such as doorways to outside halls. The change in traction does it. Also, hard soled shoes are fine, as long as they have a build up of paraffin on them. If a visitor (or pathologist) comes into the lab that doesn't have paraffin residue on the bottom of their shoes, watch out. In Texas, during the summer, walking outside will melt the paraffin off the bottom of my shoes, and the next day is slippery for a little while. Anyway, have each tech responsible for cleaning the floors in their cutting or embedding area each day, and make it part of their evaluation. Long handled scrapers are good (Mercedes Medical carries one), but my personal favorite weapon is the long edge of a glass slide (use with caution, broken glass hazard, only for experts). Those sticky, peel off mats in doorways are cool. If you have a custodial staff, have them strip your floors once a month. Waxing the floors afterward is a matter of taste, IMHO, it makes the floor more slippery than an unwaxed floor. I think mats and rugs add their own trip and fall hazards, except maybe a cushion mat in front of a grossing station, if your grosser grosses standing up. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Andrew.Prior <@t> Smith-Nephew.com Thu Feb 25 03:27:21 2010 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Thu Feb 25 03:27:32 2010 Subject: [Histonet] Re: Decalcification and processing of large bone Message-ID: <6C18ADDF244BF8439412C063019CFFEC0CD0A8E4@EHS021.wound.san> I work with bone samples trimmed to approx 35mmx35mmx4mm. Our general procedure is: Fixation: untrimmed samples stored in 10% NBF @4?C until trimming. Slices placed in fresh 10% NBF for at least 12h. Decalcification Benchtop - 10% formic acid for 10 days (change soln every 3 days), rinse for minimum 1-2 hours. Then 10% EDTA solution for 2-3 weeks (change weekly). The time in formic acid is limited at 10 days to prevent excess damage to tissue/loss of nuclear staining. The EDTA is a chelating agent and is slower at decalcifying tissue but does little damage. Microwave processor - 3 days in 10% formic acid @35?C, rinse as above, then 3 days in 10% EDTA @35?C. For both methods I recommend placing a layer of empty cassettes at the bottom of you container to allow solution to circulate under samples. After the EDTA step, it is VITAL that samples are rinsed for at least 3 hours to remove any remainng salts. These precipitate during processing and make sectioning very difficult. You may need to leave samples longer in EDTA depending on their size. There are various methods in text books for determining decalcification end point. We have a MicroCT scanner so just do a quick xray scan to check our samples. Processing. We have a Sakura Tissue Tek 5. Samples processed for 5 hours each through 50%, 70%, 90% and 3x100% Ethanol/IMS. Then 4 hours x3 changes for chloroform, then 2 hours x4 changes of wax. Sectioning Make sure block are well chilled. Some samples may need soaking in water to get good sections. We trim in samples then place face down on a melting ice block whilst trimming in the other samples. This means our samples soak and chill at the same time. If you get small areas of mineral left, you can perform surface decal with 10% fromic acid. Hope this points you in the right direction. If you can, try and go to this years NSH convention. Lots of very helpful people there, especially the Hard Tissue Committee. Best of luck. Andrew Prior Histologist Smith &Nephew Research Centre York Science Park UK Andrew.Prior@smith-nephew.com >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> ------------------------------ Message: 21 Date: Wed, 24 Feb 2010 17:34:06 -0800 (PST) From: Victor Wong Subject: [Histonet] Decalcification and processing of large bone To: Histonet@lists.utsouthwestern.edu Message-ID: <347173.34928.qm@web52706.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear all, I am going to decal and process PIG femur and lumbar vertebrates. I only have experience on soft tissues. Anyone can suggest any protocols in decalcification and processing? Many thanks in advance. Cheers, Victor >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From histonet.nospam <@t> vneubert.com Thu Feb 25 04:06:03 2010 From: histonet.nospam <@t> vneubert.com (histonet.nospam@vneubert.com) Date: Thu Feb 25 04:14:41 2010 Subject: [Histonet] Re: [ Histonet ] Tris Buffer Preservative In-Reply-To: <582736991002240859l54e7d3f2if0eeed92f51e3f1f@mail.gmail.com> Message-ID: <20100225100603.323BDC289B23@dd15630.kasserver.com> I used to sterilze my TBS by autoclaving it. For aliquots I used TBS with 0,1% sodium acide. I never had any problems with bacteria; my 10x TBS concentrate was fine for weeks. ----- Original Message ----- From: amosbrooks@gmail.com To: histonet@lists.utsouthwestern.edu Date: 24.02.2010 17:59:45 Subject: [Histonet] Tris Buffer Preservative > Hi, > ?? I have been making up my own tris buffer for immunohistochemistry. It has > been working swimmingly, with one exception. I have been doing some work on > Salmonella, and apparently the researcher has been seeing bacteria in her > immunofluorescent slides that she says are moving and seem to be viable. She > wanted to culture all my reagents. So the primary and secondary antibody > diluents (commercially purchased) came up clean, but the TBS ended up with a > pretty healthy growing colony. Now since I don't do IHC overnight in an > incubator, I don't think this is necessarily a catastrophy. (No one else has > noticed this) It does seem to warrant further investigation though. So for > the folks that make their own solutions up, what do you use as a > preservative for your buffers and how much do you use. I haven't seen > anything in any of the recipes I have found. I was thinking Sodium Azide, > but it is really hazardous, and Wikipedia says it is actually explosive ( > http://en.wikipedia.org/wiki/Sodium_azide). Biocare Medical's data sheet > says they use less than 0.25% procyclin. (thanks for not hiding the > ingredients Biocare, I love you for that!) Has anyone tried that? Any other > suggestions would be welcome. > > Thanks, > Amos > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From abright <@t> brightinstruments.com Thu Feb 25 05:21:29 2010 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Feb 25 05:21:35 2010 Subject: [Histonet] Snap Freezing Tissue In-Reply-To: <57BE698966D5C54EAE8612E8941D7683080979D6@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683080979D6@EXCHANGE3.huntingtonhospital.com> Message-ID: <3EFBB875DEE1994FB040A0B099F3AC8A0AD0C4@BRIGHT-SBS.Bright.local> Dear Laurie, We manufacture the Clini-RF Rapid Freezer =80 degs. C with options for a range of freezing methods. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: 24 February 2010 21:55 To: histonet@lists.utsouthwestern.edu Cc: Steve Pike Subject: [Histonet] Snap Freezing Tissue I was wondering how others are snap freezing tissue? We have been using a histobath (a refrigerator device that cools isopentane for freezing tissue), but it has died. I would love to have another Histobath, but they are no longer carried by the vendor that I originally bought mine from. Does anyone know where I can get either a new or refurbished Histobath (vendor calls are welcome)? I'm also interested in other freezing techniques. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 755353) is spam: Spam: http://94.126.232.8/canit/b.php?i=755353&m=c4db6e71fbd0&c=s Not spam: http://94.126.232.8/canit/b.php?i=755353&m=c4db6e71fbd0&c=n Forget vote: http://94.126.232.8/canit/b.php?i=755353&m=c4db6e71fbd0&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS From histonet.nospam <@t> vneubert.com Thu Feb 25 07:34:39 2010 From: histonet.nospam <@t> vneubert.com (histonet.nospam@vneubert.com) Date: Thu Feb 25 07:44:52 2010 Subject: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides In-Reply-To: <49D4E3E2.2010708@vneubert.com> Message-ID: <20100225133440.0AD53C289B2F@dd15630.kasserver.com> Hello Histonet, it has been a while (~10 months) since I posted a problem about uneven immuno-staining with specimen showing unstained circles after manual staining with HRP-polymere/DAB method; complete mail see below, response mails see Histonet archive (via website). Links to pictures I took: http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg The problem occured suddenly, without having changed any reagents or methods. Things I changed to avoid the unstained spots: *Adding 0,05% Tween 20 to TBS *Blocking peroxidase in coplin jar, not mounted in racks *Lowering antibody concentration which temporarily produced better results. After reviewing a big number of slides it showed up that most of the tissue affected was lung, liver and kidney which mostly means a lot of blood in the tissue when fixation in formalin starts. Erythrocytes, granulocytes and macrophages show a lot of endogenous and pseudoendogenous peroxidase activity. This is how it's done since then: Slides are taken from racks into a big coplin jar with 3% H2O2 diluted in distilled water (demineralized H2O). A slow magnetic stirrer on the bottom of the jar keeps the solution floating around the tissue, removing any O2 bubble that might appear. Slides then are remounted and rinsed a lot with TBS-T. Thank you for all your help, though it's a little late... V. Neubert, Germany ----- Original Message ----- From: histonet.nospam@vneubert.com To: histonet@lists.utsouthwestern.edu Date: 02.04.2009 18:12:18 Subject: [Histonet] Strange circles in IHC slides [...]> http://img12.imageshack.us/img12/8513/ts0402162049.jpg > http://img13.imageshack.us/img13/6514/ts0402162104.jpg [...]> > So, has ever anyone experienced sth. like this? > My conjugate control (every step except the antibody) was fine, nothing > to be seen about DAB and no circles at all. > > I used Shandon single-use coverplates, sterile buffer, fresh antibody > aliquots. Any idea? > > Thanks, > > V. Neubert From mjwilliams <@t> scilogex.com Thu Feb 25 07:55:18 2010 From: mjwilliams <@t> scilogex.com (Michael Williams) Date: Thu Feb 25 07:55:28 2010 Subject: [Histonet] Snap Freezing Tissue In-Reply-To: <57BE698966D5C54EAE8612E8941D7683080979D6@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683080979D6@EXCHANGE3.huntingtonhospital.com> Message-ID: <001901cab622$2a751560$7f5f4020$@com> Hi Laurie, We distribute Bright's Clini-RF. We hear good reports about using 3MT NovecT Engineered Fluid HFE-7100 which is inert and has an excellent environmental, health and safety profile. Regards, Michael Williams mjwilliams@scilogex.com SCILOGEX, LLC TF: (877) SCILOGEX T: (860) 828-5614 F: (860) 828-5389 www.SCILOGEX.com -----Original Message----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Wednesday, February 24, 2010 4:55 PM To: histonet@lists.utsouthwestern.edu Cc: Steve Pike Subject: [Histonet] Snap Freezing Tissue I was wondering how others are snap freezing tissue? We have been using a histobath (a refrigerator device that cools isopentane for freezing tissue), but it has died. I would love to have another Histobath, but they are no longer carried by the vendor that I originally bought mine from. Does anyone know where I can get either a new or refurbished Histobath (vendor calls are welcome)? I'm also interested in other freezing techniques. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 From andreahooper <@t> rocketmail.com Thu Feb 25 08:38:43 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Thu Feb 25 08:38:46 2010 Subject: [Histonet] Snap Freezing Tissue In-Reply-To: <858249121002241431g1452b8fbr1c665e30f8ec2d08@mail.gmail.com> Message-ID: <629761.2303.qm@web113109.mail.gq1.yahoo.com> I like this method too, and I would say it's one of the best. The only issue is high throughput. When freezing 100+ samples the floating tray becomes much more user friendly and believe it or not, the results are just as good. --- On Wed, 2/24/10, Adam . wrote: From: Adam . Subject: Re: [Histonet] Snap Freezing Tissue To: "Andrea T. Hooper" Cc: histonet@lists.utsouthwestern.edu, "Laurie Colbert" , "Steve Pike" Date: Wednesday, February 24, 2010, 10:31 PM I learned to freeze tissue by taking a beaker and filling it with 2-methylbutane and then placing that beaker in a liquid nitrogen bath. You then take place your cryomold, hold it using some long forceps, and place it on the top of the 2-methylbutane. It will freeze the entire thing in about 10 seconds. Once it's frozen, you dip the entire mold into the 2-methylbutane. Seems to work well for me. Adam From Ronald.Houston <@t> nationwidechildrens.org Thu Feb 25 08:42:13 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Feb 25 08:42:24 2010 Subject: [Histonet] Snap Freezing Tissue In-Reply-To: <001901cab622$2a751560$7f5f4020$@com> References: <57BE698966D5C54EAE8612E8941D7683080979D6@EXCHANGE3.huntingtonhospital.com> <001901cab622$2a751560$7f5f4020$@com> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B646118@chi2k3ms01.columbuschildrens.net> Who supplies the 3MT Novec Engineered Fluid HFE-7100? Thanks Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Williams Sent: Thursday, February 25, 2010 8:55 AM To: 'Laurie Colbert' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Snap Freezing Tissue Hi Laurie, We distribute Bright's Clini-RF. We hear good reports about using 3MT NovecT Engineered Fluid HFE-7100 which is inert and has an excellent environmental, health and safety profile. Regards, Michael Williams mjwilliams@scilogex.com SCILOGEX, LLC TF: (877) SCILOGEX T: (860) 828-5614 F: (860) 828-5389 www.SCILOGEX.com -----Original Message----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Wednesday, February 24, 2010 4:55 PM To: histonet@lists.utsouthwestern.edu Cc: Steve Pike Subject: [Histonet] Snap Freezing Tissue I was wondering how others are snap freezing tissue? We have been using a histobath (a refrigerator device that cools isopentane for freezing tissue), but it has died. I would love to have another Histobath, but they are no longer carried by the vendor that I originally bought mine from. Does anyone know where I can get either a new or refurbished Histobath (vendor calls are welcome)? I'm also interested in other freezing techniques. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From mjwilliams <@t> scilogex.com Thu Feb 25 09:12:43 2010 From: mjwilliams <@t> scilogex.com (Michael Williams) Date: Thu Feb 25 09:12:52 2010 Subject: [Histonet] Snap Freezing Tissue In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB21B646118@chi2k3ms01.columbuschildrens.net> References: <57BE698966D5C54EAE8612E8941D7683080979D6@EXCHANGE3.huntingtonhospital.com> <001901cab622$2a751560$7f5f4020$@com> <979FF5962E234F45B06CF0DB7C1AABB21B646118@chi2k3ms01.columbuschildrens.net> Message-ID: <001d01cab62c$fb236ea0$f16a4be0$@com> It can be purchased directly from 3M. You may also find some local suppliers. Michael Williams mjwilliams@scilogex.com SCILOGEX, LLC TF: (877) SCILOGEX T: (860) 828-5614 F: (860) 828-5389 www.SCILOGEX.com -----Original Message----- From: Houston, Ronald [mailto:Ronald.Houston@nationwidechildrens.org] Sent: Thursday, February 25, 2010 9:42 AM To: Michael Williams; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Snap Freezing Tissue Who supplies the 3MT Novec Engineered Fluid HFE-7100? Thanks Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Williams Sent: Thursday, February 25, 2010 8:55 AM To: 'Laurie Colbert' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Snap Freezing Tissue Hi Laurie, We distribute Bright's Clini-RF. We hear good reports about using 3MT NovecT Engineered Fluid HFE-7100 which is inert and has an excellent environmental, health and safety profile. Regards, Michael Williams mjwilliams@scilogex.com SCILOGEX, LLC TF: (877) SCILOGEX T: (860) 828-5614 F: (860) 828-5389 www.SCILOGEX.com -----Original Message----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Wednesday, February 24, 2010 4:55 PM To: histonet@lists.utsouthwestern.edu Cc: Steve Pike Subject: [Histonet] Snap Freezing Tissue I was wondering how others are snap freezing tissue? We have been using a histobath (a refrigerator device that cools isopentane for freezing tissue), but it has died. I would love to have another Histobath, but they are no longer carried by the vendor that I originally bought mine from. Does anyone know where I can get either a new or refurbished Histobath (vendor calls are welcome)? I'm also interested in other freezing techniques. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From rjbuesa <@t> yahoo.com Thu Feb 25 09:42:00 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 25 09:42:05 2010 Subject: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides In-Reply-To: <20100225133440.0AD53C289B2F@dd15630.kasserver.com> Message-ID: <376044.2908.qm@web65703.mail.ac4.yahoo.com> To me it seems that the sections after being picked from the water bath were not completely drained and the dewaxing process was incomplete in a way that the "round" areas kept certain amount of paraffin wax that prevented the reagents reactions. The fact that the areas are round are an?indication that water was involved, since water always leave a round imprint, due to its surface tension. I would suggest that you dewax the sections with a 2% aq. solution of dish washer soap. Dewaxing with xylene?sections containing water will be incomplete because it does not mix completely with water?but the detergent will mix with the water and will better remove the paraffin.Ren? J. ? --- On Thu, 2/25/10, histonet.nospam@vneubert.com wrote: From: histonet.nospam@vneubert.com Subject:>[Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides To: histonet@lists.utsouthwestern.edu Date: Thursday, February 25, 2010, 8:34 AM Hello Histonet, it has been a while (~10 months) since I posted a problem about uneven immuno-staining with specimen showing unstained circles after manual staining with HRP-polymere/DAB method; complete mail see below, response mails see Histonet archive (via website). Links to pictures I took: http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg The problem occured suddenly, without having changed any reagents or methods. Things I changed to avoid the unstained spots: *Adding 0,05% Tween 20 to TBS *Blocking peroxidase in coplin jar, not mounted in racks *Lowering antibody concentration which temporarily produced better results. After reviewing a big number of slides it showed up that most of the tissue affected was lung, liver and kidney which mostly means a lot of blood in the tissue when fixation in formalin starts. Erythrocytes, granulocytes and macrophages show a lot of endogenous and pseudoendogenous peroxidase activity. This is how it's done since then: Slides are taken from racks into a big coplin jar with 3% H2O2 diluted in distilled water (demineralized H2O). A slow magnetic stirrer on the bottom of the jar keeps the solution floating around the tissue, removing any O2 bubble that might appear. Slides then are remounted and rinsed a lot with TBS-T. Thank you for all your help, though it's a little late... V. Neubert, Germany ----- Original Message ----- From: histonet.nospam@vneubert.com To: histonet@lists.utsouthwestern.edu Date: 02.04.2009 18:12:18 Subject: [Histonet] Strange circles in IHC slides [...]> http://img12.imageshack.us/img12/8513/ts0402162049.jpg > http://img13.imageshack.us/img13/6514/ts0402162104.jpg [...]> > So, has ever anyone experienced sth. like this? > My conjugate control (every step except the antibody) was fine, nothing > to be seen about DAB and no circles at all. > > I used Shandon single-use coverplates, sterile buffer, fresh antibody > aliquots. Any idea? > > Thanks, > > V. Neubert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fraino3089 <@t> aol.com Thu Feb 25 10:21:04 2010 From: fraino3089 <@t> aol.com (fraino3089@aol.com) Date: Thu Feb 25 10:21:22 2010 Subject: [Histonet] Histology position at Indianapolis VA Medical center Message-ID: <8CC844DA9920339-453C-7967@webmail-d001.sysops.aol.com> Hi, There is a great hospital based Histotechnologists position now available in the great midwestern city of Indianapolis, Indiana. Follow the link provided: http://jobsearch.usajobs.gov/search.aspx?q=KB-10-SRC-320328&where=&brd=3876&vw=b&FedEmp=N&FedPub=Y&x=55&y=14 Sincerely, Nick Frain, AP supervisor Roudebush VA Medical Center Indianapolis, IN 46202 O-317-988-2217 From leiker <@t> buffalo.edu Thu Feb 25 10:42:49 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Feb 25 10:42:56 2010 Subject: [Histonet] Snap Freezing Tissue In-Reply-To: <001d01cab62c$fb236ea0$f16a4be0$@com> References: <57BE698966D5C54EAE8612E8941D7683080979D6@EXCHANGE3.huntingtonhospital.com> <001901cab622$2a751560$7f5f4020$@com> <979FF5962E234F45B06CF0DB7C1AABB21B646118@chi2k3ms01.columbuschildrens.net> <001d01cab62c$fb236ea0$f16a4be0$@com> Message-ID: <5875A35AA81CDD2A67ADD1EB@CDYwxp1931.ad.med.buffalo.edu> In response to the "interested in other freezing techniques" part of Laurie's query: We snap freeze by throwing a cyrovial of tissue (or a tissue piece wrapped in foil) into some liquid nitrogen for a couple minutes (vials will float, foil-wrapped pieces will sink). For controlled freezing of OCT-embedded tissues, I've frozen isopentane by pouring some in a liquid-nitrogen proof shallow container (such as a pipet-tip box lid) and floating it on the liquid nitrogen til frozen. I guess this works if you're processing a relatively small number of samples at a time, which, if you work in a hospital, sounds like you may not be. Additionally, we've made alcohol-dry ice slurries in styrofoam containers in which to freeze OCT-embedded tissues. Regards, Merced --On Thursday, February 25, 2010 10:12 AM -0500 Michael W illiams wrote: > It can be purchased directly from 3M. You may also find some local > suppliers. > > Michael Williams > mjwilliams@scilogex.com > SCILOGEX, LLC > TF: (877) SCILOGEX > T: (860) 828-5614 > F: (860) 828-5389 > www.SCILOGEX.com > > > -----Original Message----- > From: Houston, Ronald [mailto:Ronald.Houston@nationwidechildrens.org] > Sent: Thursday, February 25, 2010 9:42 AM > To: Michael Williams; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Snap Freezing Tissue > > Who supplies the 3MT Novec Engineered Fluid HFE-7100? > Thanks > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael > Williams > Sent: Thursday, February 25, 2010 8:55 AM > To: 'Laurie Colbert' > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Snap Freezing Tissue > > Hi Laurie, > > We distribute Bright's Clini-RF. We hear good reports about using 3MT > NovecT > Engineered Fluid HFE-7100 which is inert and has an excellent > environmental, > health and safety profile. > > Regards, > > Michael Williams > mjwilliams@scilogex.com > SCILOGEX, LLC > TF: (877) SCILOGEX > T: (860) 828-5614 > F: (860) 828-5389 > www.SCILOGEX.com > > > -----Original Message----- > From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] > Sent: Wednesday, February 24, 2010 4:55 PM > To: histonet@lists.utsouthwestern.edu > Cc: Steve Pike > Subject: [Histonet] Snap Freezing Tissue > > I was wondering how others are snap freezing tissue? We have been using > a histobath (a refrigerator device that cools isopentane for freezing > tissue), but it has died. I would love to have another Histobath, but > they are no longer carried by the vendor that I originally bought mine > from. Does anyone know where I can get either a new or refurbished > Histobath (vendor calls are welcome)? I'm also interested in other > freezing techniques. > > > > Laurie Colbert > > Huntington Hospital > > Pasadena, CA > > (626) 397-8620 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From ndevans <@t> stanford.edu Thu Feb 25 11:28:16 2010 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Thu Feb 25 11:28:23 2010 Subject: [Histonet] M.O.M frozen section background In-Reply-To: References: Message-ID: Sorry, embed in OCT in a mould sitting in a slurry. Thanks for the useful advice everyone. nick _____ From: Charles.Scouten@leica-microsystems.com [mailto:Charles.Scouten@leica-microsystems.com] Sent: Thursday, February 25, 2010 9:26 AM To: ndevans@stanford.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] M.O.M frozen section background I would say that dehydrating and rehydrating worked by washing away the leaked cytosol, but that you did not get as strong a stain inside the cells as if you had cut with a vibrating microtome. But I would agree that sucrose cryoprotection, then immersion in a slurry with dry ice would snap freeze as fast as it can be done, but wait, methanol? That will penetrate the tissue, and has its own coagulant fixative effect, no? Did you immersion freeze in methanol? Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: Nicholas David Evans [mailto:Nicholas David Evans ] Sent: Wednesday, February 24, 2010 7:01 PM To: ; Subject: RE: [Histonet] M.O.M frozen section background You made me worried there for a minute, Charles - I've spent a lot of time cryosectioning rather than using PPFE! But luckily dehydrating, rehydrating and post-fix seemed to completely get rid of the background I was experiencing. No idea why it works, but it did. (We cryoprotect In sucrose, then embed in methanol in dry ice). Best wishes Nick _____ From: Charles.Scouten@leica-microsystems.com [mailto:Charles.Scouten@leica-microsystems.com] Sent: Wednesday, February 24, 2010 2:03 PM To: ndevans@stanford.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] M.O.M frozen section background You must be using a stain for a protein or molecule present in cytosol. The frozen sections are different that the paraffin because the freezing process was too slow. Ice crystals formed, and broke membranes. Cytosol leaked into the intracellular space, and stained there. Background. Snap freeze in less than 3 seconds to solid, and the crystals cannot form. Full Immersion in isopentane (flammable) mixed into a slurry with dry ice would do it. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas David Evans Sent: Wednesday, February 24, 2010 12:34 PM To: histonet Subject: [Histonet] M.O.M frozen section background Hi All, Does anyone have any tips on reducing background staining when attempting to detect mouse monoclonal antibodies on mouse tissue? We currently use Vector's M.O.M kit - it works great on PPFE tissue with nice specific staining after pepsin unmasking, but in frozen tissue the background is much higher than the signal. I routinely use 4% PFA pre-fix, then 3% H2O2 in MeOH for 3 mins, then all the blocks that come with teh kit. I am using several different cytokeratin Abs on 10 um frozen mouse sections. The only thing that I could think to try was to dehydrate the tissues, rehydrate and try again with the unmasking, but if anyone has a better idea or commonly deals with this problem, please let me know. Best wishes Nick _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From thisisann <@t> aol.com Thu Feb 25 12:59:23 2010 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Thu Feb 25 12:59:55 2010 Subject: [Histonet] NBS/NIST Thermometers Message-ID: <8CC8463C76E2CBF-55EC-12CB@webmail-m063.sysops.aol.com> Does anyone know how frequently you have to certify your NIST Thermometers (the thermometer you use to calibrate all of the thermometers in the laboratory), including a reference. The certifiicate for my NIST Thermometer does not document the next time it needs to be calibrated. Thank you, Ann Angelo From jhabecke <@t> fhcrc.org Thu Feb 25 13:13:38 2010 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Thu Feb 25 13:13:54 2010 Subject: [Histonet] Control slides for Flag-Tag IHC Message-ID: <040346FA7309BD439C327F97D4C4D69B072C778B@ISIS.fhcrc.org> Folks, I am looking for a control for some Flag-tag IHC I am doing on FFPE tissue. I was wondering if someone could share some slides off of a block of flag transfected cells. THANKS!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org From JVanFleet <@t> dow.com Thu Feb 25 14:44:21 2010 From: JVanFleet <@t> dow.com (Van Fleet, Jonilyn (J)) Date: Thu Feb 25 14:44:39 2010 Subject: [Histonet] Control tissue for Fluoro Jade Message-ID: <11DEE9B567C45C42AEC620F3B836D53C0325E4C4@USMDLMDOWX027.dow.com> Hello Histonet, I am looking for a positive control for the Fluoro Jade Stain consisting of rodent brain tissue demonstrating neuronal necrosis in the hippocampus caused by administration of trimethyltin. Does anyone have access to a stock of controls or can anyone tell me who to contact. I have contacted the AFIP and NSH but neither were able to help. Any information would be greatly appreciated. Thank-you, Jonilyn Van Fleet, HT (ASCP) The Dow Chemical Company 989-636-3539 jvanfleet@dow.com From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Feb 25 15:32:35 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Feb 25 15:33:02 2010 Subject: [Histonet] NBS/NIST Thermometers In-Reply-To: <8CC8463C76E2CBF-55EC-12CB@webmail-m063.sysops.aol.com> References: <8CC8463C76E2CBF-55EC-12CB@webmail-m063.sysops.aol.com> Message-ID: I am in NY State and they just told me yesterday that we need to do it annually. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com [thisisann@aol.com] Sent: Thursday, February 25, 2010 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NBS/NIST Thermometers Does anyone know how frequently you have to certify your NIST Thermometers (the thermometer you use to calibrate all of the thermometers in the laboratory), including a reference. The certifiicate for my NIST Thermometer does not document the next time it needs to be calibrated. Thank you, Ann Angelo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Feb 25 15:50:42 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Feb 25 15:50:49 2010 Subject: [Histonet] Re: Snap Freezing Tissue Message-ID: In the last two or three years I and others have posted a number of times on Histonet about the Histobath (which is no longer in manufacture), Bright's Clini-RF, and 3M's NovecT Engineered Fluid HFE-7100. You can access these posts in the Archives, through Google if you wish. Bob Richmond Samurai Pathologist Knoxville TN From Janice.Mahoney <@t> alegent.org Thu Feb 25 15:55:10 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Thu Feb 25 15:55:18 2010 Subject: [Histonet] voice recognition Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C49F24@EXCHMBC2.ad.ah.local> Hello, Can anyone using voice recognition with Cerner Millennium tell me what system they are using and how they like it? Thanks, Jan Mahoney Omaha, NE ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From hana444 <@t> gmail.com Thu Feb 25 16:15:40 2010 From: hana444 <@t> gmail.com (Hana Peter) Date: Thu Feb 25 16:15:46 2010 Subject: [Histonet] haematoxylin Message-ID: <4B86F68C.70003@gmail.com> Hello everyone! Does anyone know where I can get more information about haematoxylin-haematein-oxyhaematein chemistry? I have a problem with my Harris haematoxylin modification (sodium iodate as oxidant). When I use a smaller amount of sodium iodate (about 0,1g per 1g of haematoxylin) I have a huge problem with precipitation. I don't know if this is because of the unoxidized haematoxylin in staining solution or because of something else. Any help or suggestions are welcomed! Thanks in advance! Peter From jkiernan <@t> uwo.ca Thu Feb 25 22:39:27 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Feb 25 22:39:32 2010 Subject: [Histonet] haematoxylin In-Reply-To: <4B86F68C.70003@gmail.com> References: <4B86F68C.70003@gmail.com> Message-ID: 0.1G should give you an approximately half-oxidized hamatoxylin solution along the lines of Baker's haematal-8 or haematal-16, or Gill's haemalum. It should keep well. Precipitation must be due to something else you're doing. For the chemistry a good starting point is Conn's Biological Stains (10th ed, 2002). for information, see http://biologicalstaincommission.org and click on "Publications". The current issue of Biotechnic & Histochemistry (Feb. 2010) is a special issue devoted to haematoxylin (Vol 85 No. 1). Last year the same journal (Vol 84 No. 4, pp. 159-177) carried an 18-page paper, "Nuclear staining with alum hematoxylin" by Bryan Llewellyn, which includes recipes for scores of haemalum mixtures and has much other interesting information. You can review the contents of the journal at http://informahealthcare.com/loi/bih. If your institution's library subscribes to the Informa Healthcare package of journals, you'll be able to download PDF files of articles - they go right back to 1925. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Hana Peter Date: Thursday, February 25, 2010 17:16 Subject: [Histonet] haematoxylin To: histonet@lists.utsouthwestern.edu > Hello everyone! > Does anyone know where I can get more information about > haematoxylin-haematein-oxyhaematein chemistry? > > I have a problem with my Harris haematoxylin modification > (sodium iodate as oxidant). When I use a smaller amount of > sodium iodate (about 0,1g per 1g of haematoxylin) I have a huge > problem with precipitation. I don't know if this is because of > the unoxidized haematoxylin in staining solution or because of > something else. > > Any help or suggestions are welcomed! > Thanks in advance! > Peter > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shehnazster <@t> gmail.com Fri Feb 26 05:30:41 2010 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Fri Feb 26 05:30:46 2010 Subject: [Histonet] Re: shandon's formal fixx and cytoblock kit In-Reply-To: <4fe9f16e1002200554r1ff45e23q3a1ba567c24cb8e7@mail.gmail.com> References: <4fe9f16e1002200554r1ff45e23q3a1ba567c24cb8e7@mail.gmail.com> Message-ID: <4fe9f16e1002260330j668fa3fdn2f20537f01a75745@mail.gmail.com> On Sat, Feb 20, 2010 at 3:54 PM, shehnaz khan wrote: > Hi Histonetters > > Could someone kindly share their views on the shandon's formal fixx > (concentrate) and cytoblock kit for cell block preparation in cytology? Has > it been effective for cellular preservation and cell capture from inadeduate > samples? > > Thanking you in advance. > > S .Khan > Dept of Cytology > University of Witwatersrand > Johannesburg > South Africa > From W.E.J.Hoekert <@t> olvg.nl Fri Feb 26 06:44:51 2010 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Fri Feb 26 06:49:52 2010 Subject: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides References: <376044.2908.qm@web65703.mail.ac4.yahoo.com> Message-ID: <1190CB05C44B13409483514729C2FC360C0A98@PAIT42.olvg.nl> Are you sure that you don't introduce air bubbles when you put your slides into the coverplates? The antibody will not touch the tissue if there is an air bubble. Willem Hoekert ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Rene J Buesa Verzonden: do 25-2-2010 16:42 Aan: histonet@lists.utsouthwestern.edu; histonet.nospam@vneubert.com Onderwerp: Re: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides To me it seems that the sections after being picked from the water bath were not completely drained and the dewaxing process was incomplete in a way that the "round" areas kept certain amount of paraffin wax that prevented the reagents reactions. The fact that the areas are round are an indication that water was involved, since water always leave a round imprint, due to its surface tension. I would suggest that you dewax the sections with a 2% aq. solution of dish washer soap. Dewaxing with xylene sections containing water will be incomplete because it does not mix completely with water but the detergent will mix with the water and will better remove the paraffin.Ren? J. --- On Thu, 2/25/10, histonet.nospam@vneubert.com wrote: From: histonet.nospam@vneubert.com Subject:>[Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides To: histonet@lists.utsouthwestern.edu Date: Thursday, February 25, 2010, 8:34 AM Hello Histonet, it has been a while (~10 months) since I posted a problem about uneven immuno-staining with specimen showing unstained circles after manual staining with HRP-polymere/DAB method; complete mail see below, response mails see Histonet archive (via website). Links to pictures I took: http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg The problem occured suddenly, without having changed any reagents or methods. Things I changed to avoid the unstained spots: *Adding 0,05% Tween 20 to TBS *Blocking peroxidase in coplin jar, not mounted in racks *Lowering antibody concentration which temporarily produced better results. After reviewing a big number of slides it showed up that most of the tissue affected was lung, liver and kidney which mostly means a lot of blood in the tissue when fixation in formalin starts. Erythrocytes, granulocytes and macrophages show a lot of endogenous and pseudoendogenous peroxidase activity. This is how it's done since then: Slides are taken from racks into a big coplin jar with 3% H2O2 diluted in distilled water (demineralized H2O). A slow magnetic stirrer on the bottom of the jar keeps the solution floating around the tissue, removing any O2 bubble that might appear. Slides then are remounted and rinsed a lot with TBS-T. Thank you for all your help, though it's a little late... V. Neubert, Germany ----- Original Message ----- From: histonet.nospam@vneubert.com To: histonet@lists.utsouthwestern.edu Date: 02.04.2009 18:12:18 Subject: [Histonet] Strange circles in IHC slides [...]> http://img12.imageshack.us/img12/8513/ts0402162049.jpg > http://img13.imageshack.us/img13/6514/ts0402162104.jpg [...]> > So, has ever anyone experienced sth. like this? > My conjugate control (every step except the antibody) was fine, nothing > to be seen about DAB and no circles at all. > > I used Shandon single-use coverplates, sterile buffer, fresh antibody > aliquots. Any idea? > > Thanks, > > V. Neubert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From christiegowan <@t> msn.com Fri Feb 26 08:20:34 2010 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Feb 26 08:20:40 2010 Subject: [Histonet] Fire in the lab Message-ID: Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if this type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) From DKBoyd <@t> chs.net Fri Feb 26 08:50:08 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Feb 26 08:50:17 2010 Subject: [Histonet] Fire in the lab In-Reply-To: Message-ID: Not exactly the same, but very similar. We had an automatic stainer by the sink and one of the techs was washing glassware, the stainer was running. The water apparently splashed on the wiring and a fire broke out. We jumped into action. Just as we had been in-service. You are correct what a mess to clean up! Fire extinquishers are wonderful but extremely messy. We had totally taken care of the situation by the time the fire department got here. We actually got accolades for preventing a much larger fire. It was determined that there was some exposed wires on the stainer. A good lesson for all. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net CHRISTIE GOWAN Sent by: histonet-bounces@lists.utsouthwestern.edu 02/26/2010 09:21 AM To cc Subject [Histonet] Fire in the lab Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if this type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From alexandra.meinl <@t> gmail.com Fri Feb 26 09:36:30 2010 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Fri Feb 26 09:36:35 2010 Subject: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides In-Reply-To: <1190CB05C44B13409483514729C2FC360C0A98@PAIT42.olvg.nl> References: <376044.2908.qm@web65703.mail.ac4.yahoo.com> <1190CB05C44B13409483514729C2FC360C0A98@PAIT42.olvg.nl> Message-ID: Hello, I'm glad that you already solved the problem your way. I didn't read your first post, but we had exactly the same problem (and we're also using cover plates). This artifact is very likely caused by tiny air bubbles which are trapped under the cover plate. The crucial step is when you drip a little buffer onto the plate in order to get your slide in proper position. You get much lesser air bubbles if a) no detergent is used and b) the PBS or TBS is at room temperature and not cold (which isn't good anyway). We don't use detergents anymore (on coverplates). Alexandra Meinl ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.meinl@trauma.lbg.ac.at From plucas <@t> biopath.org Fri Feb 26 10:23:31 2010 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Feb 26 10:21:28 2010 Subject: [Histonet] Saturday Tech Needed Orange County California Message-ID: <001201cab700$0987acd0$0f01a8c0@biopath.local> Hello histotechs- please email me if you're interested in working a few hours on Saturday mornings. We are flexible, and have another Saturday tech that can fill in if you can not work on certain dates. I'll give you more info privately. I'm only seeking histotechs who have at least 2 years experience embedding and cutting surgical/biopsy cases in a hospital or private lab setting. Thanks, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA From Vickroy.Jim <@t> mhsil.com Fri Feb 26 11:03:25 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Feb 26 11:03:32 2010 Subject: [Histonet] ruo antibodies Message-ID: <24A4826E8EF0964D86BC5317306F58A54257908393@mmc-mail.ad.mhsil.com> Our new CAP checklist does not mention the use of RUO antibodies anymore. This was under the question using ASR antibodies in the past. I believe the requirement was that if we wanted to use an RUO antibody we had to have a disclaimer similar to the ASR disclaimer but we also had to have a statement stating that we had searched for an IVD or ASR antibody. Does anyone know if this is still the practice or am I missing something? I do know of course than when using either an ASR or RUO antibody we have to establish and verify the performance. Any thoughts about the RUO(s)? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From rosensn <@t> live.com Fri Feb 26 11:57:50 2010 From: rosensn <@t> live.com (Stephanie Rosenwinkel) Date: Fri Feb 26 11:57:58 2010 Subject: [Histonet] Fire in the lab Message-ID: A few years back, I also had a fire break out while cleaning off the weighing instrument. There was left over powders of other chemicals on there and my gauze started on fire. So needless to say, please clean up after yourselves! It was Scary, very scary! I tried to yell for help but nothing would come out of my mouth, so I called 911. It was a lesson learned the hard way, for sure. Steph HT(ASCP) > To: christiegowan@msn.com > From: DKBoyd@chs.net > Date: Fri, 26 Feb 2010 09:50:08 -0500 > Subject: Re: [Histonet] Fire in the lab > CC: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > Not exactly the same, but very similar. We had an automatic stainer by > the sink and one of the techs was washing glassware, the stainer was > running. The water apparently splashed on the wiring and a fire broke > out. We jumped into action. Just as we had been in-service. You are > correct what a mess to clean up! Fire extinquishers are wonderful but > extremely messy. We had totally taken care of the situation by the time > the fire department got here. We actually got accolades for preventing a > much larger fire. It was determined that there was some exposed wires on > the stainer. > A good lesson for all. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: > 804-765-5582 l dkboyd@chs.net > > > > > > > > CHRISTIE GOWAN > Sent by: histonet-bounces@lists.utsouthwestern.edu > 02/26/2010 09:21 AM > > To > > cc > > Subject > [Histonet] Fire in the lab > > > > > > > > > > Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old paraffin > pot. One of my techs came in on Sunday to embed some tissues, went into > the processor room and smelled something burning. He noticed our old > paraffin pot had charred looking labels on the outside so he went over, > opened the lid and poof!!! the pot went up in flames. The thermostat had > gone haywire and heated the paraffin to flash point. Opening the lid gave > it the oxygen it needed to ignite. He triggered the alarm, made the > appropriate call and then put it out with an extinguisher. Of course it > kept re-igniting because he could not get behind it to pull the plug. The > fire dept finally was able to get it pulled out and unplugged. Needless to > say the tech was shaken and the room was a mess. I applaud his courage and > am not sure I would have done the same. There was enough xylene and > alcohol on the 4 processors to cause quite an explosion but everything > else was in a flammable cabinet. I was wondering if this type of thing had > ever happened to anyone else?? Needless to say, we have de-comissioned all > old paraffin pots and will order only those with over temp safety > features. I guess I just wanted to remind everyone that fires can happen > in the lab and do probably more often than we hear about. This was the > first time for me and I have been in this business for over 20 years. Take > care and be safe. > > Christie Gowan HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft?s powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/ From JWeems <@t> sjha.org Fri Feb 26 12:47:04 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Feb 26 12:47:16 2010 Subject: [Histonet] Fire in the lab In-Reply-To: References: Message-ID: <27648A6C5BC9B145813DF5182F83AB45052BFA@ITSSSXM01V1.one.ads.che.org> I did the same thing several years ago - caught my uniform on fire but ran to the sink and all was well in a minute - techs ran to my rescue. But then one of the gang said... "Grab the marshmallows, the supervisor's on fire!"... Gotta love em... Happy Friday!! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephanie Rosenwinkel Sent: Friday, February 26, 2010 12:58 To: dkboyd@chs.net; christiegowan@msn.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Fire in the lab A few years back, I also had a fire break out while cleaning off the weighing instrument. There was left over powders of other chemicals on there and my gauze started on fire. So needless to say, please clean up after yourselves! It was Scary, very scary! I tried to yell for help but nothing would come out of my mouth, so I called 911. It was a lesson learned the hard way, for sure. Steph HT(ASCP) > To: christiegowan@msn.com > From: DKBoyd@chs.net > Date: Fri, 26 Feb 2010 09:50:08 -0500 > Subject: Re: [Histonet] Fire in the lab > CC: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > > Not exactly the same, but very similar. We had an automatic stainer > by the sink and one of the techs was washing glassware, the stainer > was running. The water apparently splashed on the wiring and a fire broke > out. We jumped into action. Just as we had been in-service. You are > correct what a mess to clean up! Fire extinquishers are wonderful but > extremely messy. We had totally taken care of the situation by the > time the fire department got here. We actually got accolades for > preventing a much larger fire. It was determined that there was some > exposed wires on the stainer. > A good lesson for all. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional > Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l > T: 804-765-5050 l F: > 804-765-5582 l dkboyd@chs.net > > > > > > > > CHRISTIE GOWAN Sent by: > histonet-bounces@lists.utsouthwestern.edu > 02/26/2010 09:21 AM > > To > > cc > > Subject > [Histonet] Fire in the lab > > > > > > > > > > Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old > paraffin pot. One of my techs came in on Sunday to embed some tissues, > went into the processor room and smelled something burning. He noticed > our old paraffin pot had charred looking labels on the outside so he > went over, opened the lid and poof!!! the pot went up in flames. The > thermostat had gone haywire and heated the paraffin to flash point. > Opening the lid gave it the oxygen it needed to ignite. He triggered > the alarm, made the appropriate call and then put it out with an > extinguisher. Of course it kept re-igniting because he could not get > behind it to pull the plug. The fire dept finally was able to get it > pulled out and unplugged. Needless to say the tech was shaken and the > room was a mess. I applaud his courage and am not sure I would have > done the same. There was enough xylene and alcohol on the 4 processors > to cause quite an explosion but everything else was in a flammable > cabinet. I was wondering if this type of thing had ever happened to > anyone else?? Needless to say, we have de-comissioned all old paraffin > pots and will order only those with over temp safety features. I guess > I just wanted to remind everyone that fires can happen in the lab and > do probably more often than we hear about. This was the first time for > me and I have been in this business for over 20 years. Take care and be safe. > > Christie Gowan HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ---------------------------------------------------------------------- > ---- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your > computer. Do not deliver, distribute or copy this message and do not > disclose its contents or take any action in reliance on the > information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From j_amiri <@t> modares.ac.ir Fri Feb 26 13:48:20 2010 From: j_amiri <@t> modares.ac.ir (Jamshid Amiri Moghaddam) Date: Fri Feb 26 13:46:20 2010 Subject: [Histonet] special coloration for fish gill chloride cells In-Reply-To: <20100226181610.B7C24263BA@spamfilter.modares.ac.ir> References: <20100226181610.B7C24263BA@spamfilter.modares.ac.ir> Message-ID: <000001cab71c$a6584350$f308c9f0$@ac.ir> Hello friends I have some gill tissues for chloride cells localization. Do you know a method for chloride cells coloration except immunohistochemical using NKA antibody??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, February 26, 2010 9:46 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. NBS/NIST Thermometers (thisisann@aol.com) 2. Control slides for Flag-Tag IHC (Randolph-Habecker, Julie) 3. Control tissue for Fluoro Jade (Van Fleet, Jonilyn (J)) 4. RE: NBS/NIST Thermometers (McMahon, Loralee A) 5. Re: Snap Freezing Tissue (Robert Richmond) 6. voice recognition (Mahoney,Janice A) 7. haematoxylin (Hana Peter) 8. Re: haematoxylin (John Kiernan) 9. Re: shandon's formal fixx and cytoblock kit (shehnaz khan) 10. RE: [ SOLVED ][ Histonet ] Strange circles in IHC slides (Hoekert, W.E.J.) 11. Fire in the lab (CHRISTIE GOWAN) 12. Re: Fire in the lab (DKBoyd@chs.net) 13. Re: [ SOLVED ][ Histonet ] Strange circles in IHC slides (Alexandra Meinl) 14. Saturday Tech Needed Orange County California (Paula Lucas) 15. ruo antibodies (Vickroy, Jim) 16. RE: Fire in the lab (Stephanie Rosenwinkel) ---------------------------------------------------------------------- Message: 1 Date: Thu, 25 Feb 2010 13:59:23 -0500 From: thisisann@aol.com Subject: [Histonet] NBS/NIST Thermometers To: histonet@lists.utsouthwestern.edu Message-ID: <8CC8463C76E2CBF-55EC-12CB@webmail-m063.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Does anyone know how frequently you have to certify your NIST Thermometers (the thermometer you use to calibrate all of the thermometers in the laboratory), including a reference. The certifiicate for my NIST Thermometer does not document the next time it needs to be calibrated. Thank you, Ann Angelo ------------------------------ Message: 2 Date: Thu, 25 Feb 2010 11:13:38 -0800 From: "Randolph-Habecker, Julie" Subject: [Histonet] Control slides for Flag-Tag IHC To: Message-ID: <040346FA7309BD439C327F97D4C4D69B072C778B@ISIS.fhcrc.org> Content-Type: text/plain; charset="us-ascii" Folks, I am looking for a control for some Flag-tag IHC I am doing on FFPE tissue. I was wondering if someone could share some slides off of a block of flag transfected cells. THANKS!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org ------------------------------ Message: 3 Date: Thu, 25 Feb 2010 15:44:21 -0500 From: "Van Fleet, Jonilyn (J)" Subject: [Histonet] Control tissue for Fluoro Jade To: Message-ID: <11DEE9B567C45C42AEC620F3B836D53C0325E4C4@USMDLMDOWX027.dow.com> Content-Type: text/plain; charset="us-ascii" Hello Histonet, I am looking for a positive control for the Fluoro Jade Stain consisting of rodent brain tissue demonstrating neuronal necrosis in the hippocampus caused by administration of trimethyltin. Does anyone have access to a stock of controls or can anyone tell me who to contact. I have contacted the AFIP and NSH but neither were able to help. Any information would be greatly appreciated. Thank-you, Jonilyn Van Fleet, HT (ASCP) The Dow Chemical Company 989-636-3539 jvanfleet@dow.com ------------------------------ Message: 4 Date: Thu, 25 Feb 2010 16:32:35 -0500 From: "McMahon, Loralee A" Subject: RE: [Histonet] NBS/NIST Thermometers To: "thisisann@aol.com" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am in NY State and they just told me yesterday that we need to do it annually. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com [thisisann@aol.com] Sent: Thursday, February 25, 2010 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NBS/NIST Thermometers Does anyone know how frequently you have to certify your NIST Thermometers (the thermometer you use to calibrate all of the thermometers in the laboratory), including a reference. The certifiicate for my NIST Thermometer does not document the next time it needs to be calibrated. Thank you, Ann Angelo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 25 Feb 2010 16:50:42 -0500 From: Robert Richmond Subject: [Histonet] Re: Snap Freezing Tissue To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 In the last two or three years I and others have posted a number of times on Histonet about the Histobath (which is no longer in manufacture), Bright's Clini-RF, and 3M's NovecT Engineered Fluid HFE-7100. You can access these posts in the Archives, through Google if you wish. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 6 Date: Thu, 25 Feb 2010 15:55:10 -0600 From: "Mahoney,Janice A" Subject: [Histonet] voice recognition To: "histonet@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C49F24@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Hello, Can anyone using voice recognition with Cerner Millennium tell me what system they are using and how they like it? Thanks, Jan Mahoney Omaha, NE ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 7 Date: Thu, 25 Feb 2010 23:15:40 +0100 From: Hana Peter Subject: [Histonet] haematoxylin To: histonet@lists.utsouthwestern.edu Message-ID: <4B86F68C.70003@gmail.com> Content-Type: text/plain; charset=ISO-8859-2; format=flowed Hello everyone! Does anyone know where I can get more information about haematoxylin-haematein-oxyhaematein chemistry? I have a problem with my Harris haematoxylin modification (sodium iodate as oxidant). When I use a smaller amount of sodium iodate (about 0,1g per 1g of haematoxylin) I have a huge problem with precipitation. I don't know if this is because of the unoxidized haematoxylin in staining solution or because of something else. Any help or suggestions are welcomed! Thanks in advance! Peter ------------------------------ Message: 8 Date: Thu, 25 Feb 2010 23:39:27 -0500 From: John Kiernan Subject: Re: [Histonet] haematoxylin To: Hana Peter Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; CHARSET=US-ASCII 0.1G should give you an approximately half-oxidized hamatoxylin solution along the lines of Baker's haematal-8 or haematal-16, or Gill's haemalum. It should keep well. Precipitation must be due to something else you're doing. For the chemistry a good starting point is Conn's Biological Stains (10th ed, 2002). for information, see http://biologicalstaincommission.org and click on "Publications". The current issue of Biotechnic & Histochemistry (Feb. 2010) is a special issue devoted to haematoxylin (Vol 85 No. 1). Last year the same journal (Vol 84 No. 4, pp. 159-177) carried an 18-page paper, "Nuclear staining with alum hematoxylin" by Bryan Llewellyn, which includes recipes for scores of haemalum mixtures and has much other interesting information. You can review the contents of the journal at http://informahealthcare.com/loi/bih. If your institution's library subscribes to the Informa Healthcare package of journals, you'll be able to download PDF files of articles - they go right back to 1925. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Hana Peter Date: Thursday, February 25, 2010 17:16 Subject: [Histonet] haematoxylin To: histonet@lists.utsouthwestern.edu > Hello everyone! > Does anyone know where I can get more information about > haematoxylin-haematein-oxyhaematein chemistry? > > I have a problem with my Harris haematoxylin modification > (sodium iodate as oxidant). When I use a smaller amount of > sodium iodate (about 0,1g per 1g of haematoxylin) I have a huge > problem with precipitation. I don't know if this is because of > the unoxidized haematoxylin in staining solution or because of > something else. > > Any help or suggestions are welcomed! > Thanks in advance! > Peter > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 26 Feb 2010 13:30:41 +0200 From: shehnaz khan Subject: [Histonet] Re: shandon's formal fixx and cytoblock kit To: histonet@lists.utsouthwestern.edu Message-ID: <4fe9f16e1002260330j668fa3fdn2f20537f01a75745@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 On Sat, Feb 20, 2010 at 3:54 PM, shehnaz khan wrote: > Hi Histonetters > > Could someone kindly share their views on the shandon's formal fixx > (concentrate) and cytoblock kit for cell block preparation in cytology? Has > it been effective for cellular preservation and cell capture from inadeduate > samples? > > Thanking you in advance. > > S .Khan > Dept of Cytology > University of Witwatersrand > Johannesburg > South Africa > ------------------------------ Message: 10 Date: Fri, 26 Feb 2010 13:44:51 +0100 From: "Hoekert, W.E.J." Subject: RE: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides To: "Rene J Buesa" , , Message-ID: <1190CB05C44B13409483514729C2FC360C0A98@PAIT42.olvg.nl> Content-Type: text/plain; charset="iso-8859-1" Are you sure that you don't introduce air bubbles when you put your slides into the coverplates? The antibody will not touch the tissue if there is an air bubble. Willem Hoekert ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Rene J Buesa Verzonden: do 25-2-2010 16:42 Aan: histonet@lists.utsouthwestern.edu; histonet.nospam@vneubert.com Onderwerp: Re: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides To me it seems that the sections after being picked from the water bath were not completely drained and the dewaxing process was incomplete in a way that the "round" areas kept certain amount of paraffin wax that prevented the reagents reactions. The fact that the areas are round are an indication that water was involved, since water always leave a round imprint, due to its surface tension. I would suggest that you dewax the sections with a 2% aq. solution of dish washer soap. Dewaxing with xylene sections containing water will be incomplete because it does not mix completely with water but the detergent will mix with the water and will better remove the paraffin.Reni J. --- On Thu, 2/25/10, histonet.nospam@vneubert.com wrote: From: histonet.nospam@vneubert.com Subject:>[Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides To: histonet@lists.utsouthwestern.edu Date: Thursday, February 25, 2010, 8:34 AM Hello Histonet, it has been a while (~10 months) since I posted a problem about uneven immuno-staining with specimen showing unstained circles after manual staining with HRP-polymere/DAB method; complete mail see below, response mails see Histonet archive (via website). Links to pictures I took: http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg The problem occured suddenly, without having changed any reagents or methods. Things I changed to avoid the unstained spots: *Adding 0,05% Tween 20 to TBS *Blocking peroxidase in coplin jar, not mounted in racks *Lowering antibody concentration which temporarily produced better results. After reviewing a big number of slides it showed up that most of the tissue affected was lung, liver and kidney which mostly means a lot of blood in the tissue when fixation in formalin starts. Erythrocytes, granulocytes and macrophages show a lot of endogenous and pseudoendogenous peroxidase activity. This is how it's done since then: Slides are taken from racks into a big coplin jar with 3% H2O2 diluted in distilled water (demineralized H2O). A slow magnetic stirrer on the bottom of the jar keeps the solution floating around the tissue, removing any O2 bubble that might appear. Slides then are remounted and rinsed a lot with TBS-T. Thank you for all your help, though it's a little late... V. Neubert, Germany ----- Original Message ----- From: histonet.nospam@vneubert.com To: histonet@lists.utsouthwestern.edu Date: 02.04.2009 18:12:18 Subject: [Histonet] Strange circles in IHC slides [...]> http://img12.imageshack.us/img12/8513/ts0402162049.jpg > http://img13.imageshack.us/img13/6514/ts0402162104.jpg [...]> > So, has ever anyone experienced sth. like this? > My conjugate control (every step except the antibody) was fine, nothing > to be seen about DAB and no circles at all. > > I used Shandon single-use coverplates, sterile buffer, fresh antibody > aliquots. Any idea? > > Thanks, > > V. Neubert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ------------------------------ Message: 11 Date: Fri, 26 Feb 2010 14:20:34 +0000 From: CHRISTIE GOWAN Subject: [Histonet] Fire in the lab To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if thi s type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) ------------------------------ Message: 12 Date: Fri, 26 Feb 2010 09:50:08 -0500 From: DKBoyd@chs.net Subject: Re: [Histonet] Fire in the lab To: CHRISTIE GOWAN Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Not exactly the same, but very similar. We had an automatic stainer by the sink and one of the techs was washing glassware, the stainer was running. The water apparently splashed on the wiring and a fire broke out. We jumped into action. Just as we had been in-service. You are correct what a mess to clean up! Fire extinquishers are wonderful but extremely messy. We had totally taken care of the situation by the time the fire department got here. We actually got accolades for preventing a much larger fire. It was determined that there was some exposed wires on the stainer. A good lesson for all. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net CHRISTIE GOWAN Sent by: histonet-bounces@lists.utsouthwestern.edu 02/26/2010 09:21 AM To cc Subject [Histonet] Fire in the lab Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if this type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 13 Date: Fri, 26 Feb 2010 16:36:30 +0100 From: Alexandra Meinl Subject: Re: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides To: histonet@lists.utsouthwestern.edu, histonet.nospam@vneubert.com Message-ID: Content-Type: text/plain; charset=UTF-8 Hello, I'm glad that you already solved the problem your way. I didn't read your first post, but we had exactly the same problem (and we're also using cover plates). This artifact is very likely caused by tiny air bubbles which are trapped under the cover plate. The crucial step is when you drip a little buffer onto the plate in order to get your slide in proper position. You get much lesser air bubbles if a) no detergent is used and b) the PBS or TBS is at room temperature and not cold (which isn't good anyway). We don't use detergents anymore (on coverplates). Alexandra Meinl ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.meinl@trauma.lbg.ac.at ------------------------------ Message: 14 Date: Fri, 26 Feb 2010 08:23:31 -0800 From: "Paula Lucas" Subject: [Histonet] Saturday Tech Needed Orange County California To: Message-ID: <001201cab700$0987acd0$0f01a8c0@biopath.local> Content-Type: text/plain; charset="us-ascii" Hello histotechs- please email me if you're interested in working a few hours on Saturday mornings. We are flexible, and have another Saturday tech that can fill in if you can not work on certain dates. I'll give you more info privately. I'm only seeking histotechs who have at least 2 years experience embedding and cutting surgical/biopsy cases in a hospital or private lab setting. Thanks, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA ------------------------------ Message: 15 Date: Fri, 26 Feb 2010 11:03:25 -0600 From: "Vickroy, Jim" Subject: [Histonet] ruo antibodies To: "Histonet@lists.utsouthwestern.edu" Message-ID: <24A4826E8EF0964D86BC5317306F58A54257908393@mmc-mail.ad.mhsil.com> Content-Type: text/plain; charset="us-ascii" Our new CAP checklist does not mention the use of RUO antibodies anymore. This was under the question using ASR antibodies in the past. I believe the requirement was that if we wanted to use an RUO antibody we had to have a disclaimer similar to the ASR disclaimer but we also had to have a statement stating that we had searched for an IVD or ASR antibody. Does anyone know if this is still the practice or am I missing something? I do know of course than when using either an ASR or RUO antibody we have to establish and verify the performance. Any thoughts about the RUO(s)? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 16 Date: Fri, 26 Feb 2010 11:57:50 -0600 From: Stephanie Rosenwinkel Subject: RE: [Histonet] Fire in the lab To: , Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="Windows-1252" A few years back, I also had a fire break out while cleaning off the weighing instrument. There was left over powders of other chemicals on there and my gauze started on fire. So needless to say, please clean up after yourselves! It was Scary, very scary! I tried to yell for help but nothing would come out of my mouth, so I called 911. It was a lesson learned the hard way, for sure. Steph HT(ASCP) > To: christiegowan@msn.com > From: DKBoyd@chs.net > Date: Fri, 26 Feb 2010 09:50:08 -0500 > Subject: Re: [Histonet] Fire in the lab > CC: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > Not exactly the same, but very similar. We had an automatic stainer by > the sink and one of the techs was washing glassware, the stainer was > running. The water apparently splashed on the wiring and a fire broke > out. We jumped into action. Just as we had been in-service. You are > correct what a mess to clean up! Fire extinquishers are wonderful but > extremely messy. We had totally taken care of the situation by the time > the fire department got here. We actually got accolades for preventing a > much larger fire. It was determined that there was some exposed wires on > the stainer. > A good lesson for all. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: > 804-765-5582 l dkboyd@chs.net > > > > > > > > CHRISTIE GOWAN > Sent by: histonet-bounces@lists.utsouthwestern.edu > 02/26/2010 09:21 AM > > To > > cc > > Subject > [Histonet] Fire in the lab > > > > > > > > > > Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old paraffin > pot. One of my techs came in on Sunday to embed some tissues, went into > the processor room and smelled something burning. He noticed our old > paraffin pot had charred looking labels on the outside so he went over, > opened the lid and poof!!! the pot went up in flames. The thermostat had > gone haywire and heated the paraffin to flash point. Opening the lid gave > it the oxygen it needed to ignite. He triggered the alarm, made the > appropriate call and then put it out with an extinguisher. Of course it > kept re-igniting because he could not get behind it to pull the plug. The > fire dept finally was able to get it pulled out and unplugged. Needless to > say the tech was shaken and the room was a mess. I applaud his courage and > am not sure I would have done the same. There was enough xylene and > alcohol on the 4 processors to cause quite an explosion but everything > else was in a flammable cabinet. I was wondering if this type of thing had > ever happened to anyone else?? Needless to say, we have de-comissioned all > old paraffin pots and will order only those with over temp safety > features. I guess I just wanted to remind everyone that fires can happen > in the lab and do probably more often than we hear about. This was the > first time for me and I have been in this business for over 20 years. Take > care and be safe. > > Christie Gowan HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsofts powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 37 **************************************** From POWELL_SA <@t> mercer.edu Fri Feb 26 13:59:42 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Feb 26 13:59:48 2010 Subject: [Histonet] Fire in the lab In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE2242A3E5782@MERCERMAIL.MercerU.local> One of those chemicals could have been chromic acid which will ignite when alcohol is introduced. One of my techs cleaned up the counter with alcohol after measuring chromic acid and we all ran for the extinguisher. She just stood there and shook her hands and stared at the flames. This was right next to all the alcohols, xylenes and stains for the H&E, we did not have automation back then. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephanie Rosenwinkel Sent: Friday, February 26, 2010 12:58 PM To: dkboyd@chs.net; christiegowan@msn.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Fire in the lab A few years back, I also had a fire break out while cleaning off the weighing instrument. There was left over powders of other chemicals on there and my gauze started on fire. So needless to say, please clean up after yourselves! It was Scary, very scary! I tried to yell for help but nothing would come out of my mouth, so I called 911. It was a lesson learned the hard way, for sure. Steph HT(ASCP) > To: christiegowan@msn.com > From: DKBoyd@chs.net > Date: Fri, 26 Feb 2010 09:50:08 -0500 > Subject: Re: [Histonet] Fire in the lab > CC: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > Not exactly the same, but very similar. We had an automatic stainer by > the sink and one of the techs was washing glassware, the stainer was > running. The water apparently splashed on the wiring and a fire broke > out. We jumped into action. Just as we had been in-service. You are > correct what a mess to clean up! Fire extinquishers are wonderful but > extremely messy. We had totally taken care of the situation by the time > the fire department got here. We actually got accolades for preventing a > much larger fire. It was determined that there was some exposed wires on > the stainer. > A good lesson for all. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: > 804-765-5582 l dkboyd@chs.net > > > > > > > > CHRISTIE GOWAN > Sent by: histonet-bounces@lists.utsouthwestern.edu > 02/26/2010 09:21 AM > > To > > cc > > Subject > [Histonet] Fire in the lab > > > > > > > > > > Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old paraffin > pot. One of my techs came in on Sunday to embed some tissues, went into > the processor room and smelled something burning. He noticed our old > paraffin pot had charred looking labels on the outside so he went over, > opened the lid and poof!!! the pot went up in flames. The thermostat had > gone haywire and heated the paraffin to flash point. Opening the lid gave > it the oxygen it needed to ignite. He triggered the alarm, made the > appropriate call and then put it out with an extinguisher. Of course it > kept re-igniting because he could not get behind it to pull the plug. The > fire dept finally was able to get it pulled out and unplugged. Needless to > say the tech was shaken and the room was a mess. I applaud his courage and > am not sure I would have done the same. There was enough xylene and > alcohol on the 4 processors to cause quite an explosion but everything > else was in a flammable cabinet. I was wondering if this type of thing had > ever happened to anyone else?? Needless to say, we have de-comissioned all > old paraffin pots and will order only those with over temp safety > features. I guess I just wanted to remind everyone that fires can happen > in the lab and do probably more often than we hear about. This was the > first time for me and I have been in this business for over 20 years. Take > care and be safe. > > Christie Gowan HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Fri Feb 26 14:10:49 2010 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Fri Feb 26 14:10:54 2010 Subject: [Histonet] Crystal/Mount Message-ID: I know this has been asked before. Where can I purchase Crystal/Mount mounting media? Thanks Ruth N.I.H. From rjbuesa <@t> yahoo.com Fri Feb 26 14:28:18 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 26 14:28:23 2010 Subject: [Histonet] Crystal/Mount In-Reply-To: Message-ID: <93234.99967.qm@web65703.mail.ac4.yahoo.com> Why you just don't Google it? Ren? J. --- On Fri, 2/26/10, Yaskovich, Ruth A (NIH/NIDCR) [E] wrote: From: Yaskovich, Ruth A (NIH/NIDCR) [E] Subject: [Histonet] Crystal/Mount To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 26, 2010, 3:10 PM I know this has been asked before. Where can I purchase Crystal/Mount mounting media? Thanks Ruth N.I.H. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Fri Feb 26 14:38:29 2010 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Fri Feb 26 14:38:50 2010 Subject: [Histonet] SOP for Room Temp./Humidity Monitors Message-ID: <8CC853AC9B86DFF-2C60-647@webmail-d077.sysops.aol.com> Does anyone have an SOP for monitoring room temp and humidity they can share with me? Thank you, Ann From dmccaig <@t> ckha.on.ca Fri Feb 26 14:45:35 2010 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Feb 26 14:45:43 2010 Subject: [Histonet] minimum fixation time for needle core prostate biopsies Message-ID: Is there a minimum time for prostate biopsies to be fixed. Does fixation times affect PIN4's? Diana From cparsons <@t> vt.edu Fri Feb 26 14:50:17 2010 From: cparsons <@t> vt.edu (Parsons, Catherine) Date: Fri Feb 26 14:50:20 2010 Subject: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines Message-ID: I work in a small research lab, and we are shopping for a new tissue embedding machine. Our current unit is a very old AutoTechnicon carousel style system. Space is limited, and we do not have large numbers or constant flow of samples to embed, so we are happy to stay with the carousel style. I would be very interested to hear some reviews of some of the modern models in terms of quality and reliability, as well as ease of use. I have literature on the Leica TP1020 and Thermo Shandon Citadel. Does anyone have others to suggest? Thanks, Cathy From cparsons <@t> vt.edu Fri Feb 26 15:15:54 2010 From: cparsons <@t> vt.edu (Parsons, Catherine) Date: Fri Feb 26 15:15:59 2010 Subject: FW: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines Message-ID: Thanks for catching my typo..., we are looking for a carousel style tissue processor. Cathy From: Richard Yeo [mailto:ryeo@wchosp.org] Sent: Friday, February 26, 2010 4:04 PM To: Parsons, Catherine Subject: RE: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines You are talking about processors. Embedding centers are not carousel design. Do you need info on embedding stations or processors? Rich Y ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Parsons, Catherine Sent: Fri 2/26/2010 3:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines I work in a small research lab, and we are shopping for a new tissue embedding machine. Our current unit is a very old AutoTechnicon carousel style system. Space is limited, and we do not have large numbers or constant flow of samples to embed, so we are happy to stay with the carousel style. I would be very interested to hear some reviews of some of the modern models in terms of quality and reliability, as well as ease of use. I have literature on the Leica TP1020 and Thermo Shandon Citadel. Does anyone have others to suggest? Thanks, Cathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGE AND CONFIDENTIALITY NOTICE The information in this electronic mail is intended for the named recipients only. It may contain privileged and confidential material and may be protected under law by the Health Insurance Portability and Accountability Act. Any use of this information by anyone other than the intended receiver is prohibited. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately by replying to this electronic e-mail. Please delete it from your computer. Thank you From rjbuesa <@t> yahoo.com Fri Feb 26 15:31:32 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 26 15:31:37 2010 Subject: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines In-Reply-To: Message-ID: <359923.43944.qm@web65716.mail.ac4.yahoo.com> The Leica TP1020 is a good and reliable instrument. Ren? J. --- On Fri, 2/26/10, Parsons, Catherine wrote: From: Parsons, Catherine Subject: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 26, 2010, 3:50 PM I work in a small research lab, and we are shopping for a new tissue embedding machine.? Our current unit is a very old AutoTechnicon carousel style system.? Space is limited, and we do not have large numbers or constant flow of samples to embed, so we are happy to stay with the carousel style.? I would be very interested to hear some reviews of some of the modern models in terms of quality and reliability, as well as ease of use.? I have literature on the Leica TP1020 and Thermo Shandon Citadel.? Does anyone have others to suggest? Thanks, Cathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 26 15:33:02 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 26 15:33:09 2010 Subject: [Histonet] minimum fixation time for needle core prostate biopsies In-Reply-To: Message-ID: <612684.12545.qm@web65714.mail.ac4.yahoo.com> A poor fixation will affect all the cell components. You may have a result but if the fixation is incomplete, the results will not indicate the real amount. At least 8 hours of fixation will be required. Ren? J. --- On Fri, 2/26/10, Diana McCaig wrote: From: Diana McCaig Subject: [Histonet] minimum fixation time for needle core prostate biopsies To: histonet@lists.utsouthwestern.edu Date: Friday, February 26, 2010, 3:45 PM Is there a minimum time for prostate biopsies to be fixed.? Does fixation times affect PIN4's? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Feb 26 16:46:26 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Feb 26 16:46:41 2010 Subject: [Histonet] Crystal/Mount In-Reply-To: <93234.99967.qm@web65703.mail.ac4.yahoo.com> References: <93234.99967.qm@web65703.mail.ac4.yahoo.com> Message-ID: <471D11E9F3F1410187E36FC3EB9DED02@JoePC> Thermo Fisher or whatever they are called this week has it Joe ----- Original Message ----- From: "Rene J Buesa" To: ; " Ruth A (NIH/NIDCR) [E]Yaskovich" Sent: Friday, February 26, 2010 2:28 PM Subject: Re: [Histonet] Crystal/Mount Why you just don't Google it? Ren? J. --- On Fri, 2/26/10, Yaskovich, Ruth A (NIH/NIDCR) [E] wrote: From: Yaskovich, Ruth A (NIH/NIDCR) [E] Subject: [Histonet] Crystal/Mount To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 26, 2010, 3:10 PM I know this has been asked before. Where can I purchase Crystal/Mount mounting media? Thanks Ruth N.I.H. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Feb 26 16:51:16 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Feb 26 16:51:27 2010 Subject: [Histonet] Fire in the lab In-Reply-To: References: Message-ID: Once upon a time in a far away land, we used to boil our embedding molds in boiling soapy water, over an open Bunsen burner, followed by an alcohol rinse then air dry. One time the fire alarm was activated and we had to evacuate the hospital. We were out there quit awhile. When we received the all clear to go back into the hospital, I was the first one back in the lab and the fire department was there, looking into our pot that had boiled out and was smoking up the lab. This wasn't the cause of the first alarm, but it did set off the second. Joe ----- Original Message ----- From: "CHRISTIE GOWAN" To: Sent: Friday, February 26, 2010 8:20 AM Subject: [Histonet] Fire in the lab Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if this type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Sat Feb 27 01:10:42 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Feb 27 01:10:46 2010 Subject: [Histonet] Crystal/Mount In-Reply-To: <93234.99967.qm@web65703.mail.ac4.yahoo.com> References: <93234.99967.qm@web65703.mail.ac4.yahoo.com> Message-ID: You're quite right Ren? . Googling "crystalmount" brings up a helpful item (for a competing mounting medium) at the top of the heap, with detailed instructions for use. The third and fifth hits are also helpful. Unfortunately these are followed by hundreds of items, most having nothing to do with microtechnique! The refractive index of CrystalMount is very low (1.35) but it increases with drying to something close to that of a regular resinous mountant (>1.5). See http://www.proscitech.com.au It is unfortunate that the compositions of most resinous mounting media are trade secrets. It's possible (in principle) to make up your own Canada balsam (very expensive) or DPX (might not work; bought DPX is sometimes NBG!). There are plenty of aqueous mountants that can easily be made in any lab or kitchen, some with refractive indices >1.5. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Rene J Buesa rjbuesa@yahoo.com > Why you just don't Google it? > Ren? J. > > --- On Fri, 2/26/10, Yaskovich, Ruth A (NIH/NIDCR) [E] > wrote: > > > From: Yaskovich, Ruth A (NIH/NIDCR) [E] > Subject: [Histonet] Crystal/Mount > > I know this has been asked before. Where can I purchase > Crystal/Mount mounting media? > Thanks > Ruth N.I.H. From Anna.Inman <@t> stmarygj.org Sat Feb 27 09:34:40 2010 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Sat Feb 27 09:34:59 2010 Subject: [Histonet] patient ID Message-ID: <2925AE271EAAD440AF48FCCEB8002D091443DD5B@smgmail01.smgj.sclhs.net> I know this gets discussed frequently but we are still struggling with the new CAP requirement of 2 patient identifiers at the time of collection. Most of our workload is generated from out patient (clinics, physician/clinician offices, etc) - we have sent letters and offered educational sessions to discuss the importance of this but the question is what to do if we do not get compliance??? I haven't, yet, heard back from the CAP but am wondering how everyone else is addressing this issue? Thank you for your feedback :-) Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From louise.renton <@t> gmail.com Sat Feb 27 12:51:00 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Sat Feb 27 12:51:05 2010 Subject: [Histonet] Fire in the lab In-Reply-To: References: Message-ID: hey, I once set my hair on fire in the lab...singed off my eyebrows and burnt my nostril hair. It took several days for the burnt hair smell to get out of my nose!!. This too was in a time long long ago in another time dimension On Sat, Feb 27, 2010 at 12:51 AM, Joe Nocito wrote: > Once upon a time in a far away land, we used to boil our embedding molds in > boiling soapy water, over an open Bunsen burner, followed by an alcohol > rinse then air dry. One time the fire alarm was activated and we had to > evacuate the hospital. We were out there quit awhile. When we received the > all clear to go back into the hospital, I was the first one back in the lab > and the fire department was there, looking into our pot that had boiled out > and was smoking up the lab. This wasn't the cause of the first alarm, but it > did set off the second. > > Joe > ----- Original Message ----- From: "CHRISTIE GOWAN" > > > To: > Sent: Friday, February 26, 2010 8:20 AM > > Subject: [Histonet] Fire in the lab > > > > > > Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old paraffin > pot. One of my techs came in on Sunday to embed some tissues, went into the > processor room and smelled something burning. He noticed our old paraffin > pot had charred looking labels on the outside so he went over, opened the > lid and poof!!! the pot went up in flames. The thermostat had gone haywire > and heated the paraffin to flash point. Opening the lid gave it the oxygen > it needed to ignite. He triggered the alarm, made the appropriate call and > then put it out with an extinguisher. Of course it kept re-igniting because > he could not get behind it to pull the plug. The fire dept finally was able > to get it pulled out and unplugged. Needless to say the tech was shaken and > the room was a mess. I applaud his courage and am not sure I would have done > the same. There was enough xylene and alcohol on the 4 processors to cause > quite an explosion but everything else was in a flammable cabinet. I was > wondering if this type of thing had ever happened to anyone else?? Needless > to say, we have de-comissioned all old paraffin pots and will order only > those with over temp safety features. I guess I just wanted to remind > everyone that fires can happen in the lab and do probably more often than we > hear about. This was the first time for me and I have been in this business > for over 20 years. Take care and be safe. > > Christie Gowan HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From pruegg <@t> ihctech.net Sat Feb 27 13:47:01 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Feb 27 13:47:46 2010 Subject: SPAM-LOW: [Histonet] ruo antibodies In-Reply-To: <24A4826E8EF0964D86BC5317306F58A54257908393@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A54257908393@mmc-mail.ad.mhsil.com> Message-ID: <5E6E0AB0ED28430797B6300065C77E6D@prueggihctechlt> Yea we were just looking at this yesterday. There is no question on the CAP check list about using RUO's anymore, but it is still in the discussion section with ASR's and it is stated as you mentioned to include a disclaimer and statement that you did try to find an IVD or ASR antibody. Our question was about billing the patient for RUO use. CAP does not really address billing issues like that, but CMS does as far as we could tell, and we have been told that if you bill for RUO's CMS could come back and deny payment of the claim and perhaps all bills from your lab. We were wondering if anyone is billing patients for RUO antibodies, but of course if you are you may not want to say so to bring attention to yourself. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Friday, February 26, 2010 10:03 AM To: Histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] ruo antibodies Our new CAP checklist does not mention the use of RUO antibodies anymore. This was under the question using ASR antibodies in the past. I believe the requirement was that if we wanted to use an RUO antibody we had to have a disclaimer similar to the ASR disclaimer but we also had to have a statement stating that we had searched for an IVD or ASR antibody. Does anyone know if this is still the practice or am I missing something? I do know of course than when using either an ASR or RUO antibody we have to establish and verify the performance. Any thoughts about the RUO(s)? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathmaster <@t> yahoo.com Sat Feb 27 14:30:35 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Sat Feb 27 14:30:40 2010 Subject: [Histonet] Fire in the lab Message-ID: <442926.94178.qm@web111115.mail.gq1.yahoo.com> Here's mine. Had a per diem tech who?didn't like the electric forceps warmer.? She worked over Saturdays alone and brought in an alcohol lamp to heat her forceps and then knocked it over spilling flaming alcohol all over the thermal and dispensing consoles. Then, even worse, she poured water all over both plugged- in electic appliances. We got away with just melted facade?face plates on both instruments. ? Jeff Silverman From relia1 <@t> earthlink.net Sat Feb 27 21:12:50 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Sat Feb 27 21:12:55 2010 Subject: [Histonet] RELIA Histology Careers Bulletin 2/28/10 Are you into Facebook? MySpace? Twitter? or Linkedin? Message-ID: Hi Histonetters!! Facebook, Myspace, Linkedin, Twitter; Have you joined the Social Networking Craze? If so then you know how much fun it can be reconnecting with old friends, making new ones. If you do have an account on one of these networks I would love to connect with you. Please shoot me back an e-mail with your id on the site or an invitation to connect/friend/follow and I will respond right away. If that was like a foreign language to you and you would like some help using any of these sites please let me know. I would be happy to help you join in. I also wanted to tell you about my current job openings. All of these jobs are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance. Need help with your resume or following up after the interview or negotiating salary? Give me a call or shoot me an e-mail. I would be happy to help out. This is a complimentary service of RELIA of course. Here is a list of my current open positions HISTOLOGY/PATHOLOGY MANAGEMENT NY- Orange/Rockland County Histology Supervisor MA - Cape Cod Pathology Supervisor OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA - Central CA - Pathology Supervisor HISTOTECHS FL-Miami Histotechnologist needed for growing private lab GA-Grossing Histotechnologist Night Shift Great Shift diff MA-Boston - Immunohistochemistry Specialist MA-Boston - Histotechs day and evening shift NY-Orange/Rockland County Brand New Lab NYS license or Elig 2nd shift. CA-Los Angeles Histotechnologist afternoon shift PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC - Charlotte PA grad from NAACLES program required FL- Miami Growing private lab MARKETING AND SALES Marketing Rep - Histo/Cyto Products Pacific NW Region CYTOLOGY PA-Pittsburgh Cytology Supervisor If you or any of your friends would like more information on any of the positions listed or help with a job search in another area please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember it never hurts to look. Hope to hear from you soon. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia From tkngflght <@t> yahoo.com Sun Feb 28 19:14:17 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sun Feb 28 19:14:24 2010 Subject: [Histonet] Fire in the lab Message-ID: <595933.44936.qm@web50901.mail.re2.yahoo.com> Amazing that your tech had the presence of mind to do ALL of those things! ? Used to work with a couple of old school techs back in the days when folks smoked in the lab.? One tech would embed with a xylene soaked rag to wipe the plate and a cigarette hanging out of her mouth.? She also used an open bunsen burner to keep her forceps hot....cannot tell you how many times she set fire to that rag...used her coffee to put it out most times... ? As Louise says--in another time and dimension. ? Cheryl --- On Sun, 2/28/10, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 39 To: histonet@lists.utsouthwestern.edu Date: Sunday, February 28, 2010, 10:01 AM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. Re: Fire in the lab (louise renton) ???2. RE: SPAM-LOW:? [Histonet] ruo antibodies (Patsy Ruegg) ???3. Fire in the lab (Jeffrey Silverman) ???4. RELIA Histology Careers Bulletin 2/28/10 Are you into ? ? ? Facebook? MySpace? Twitter? or Linkedin? (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Sat, 27 Feb 2010 20:51:00 +0200 From: louise renton Subject: Re: [Histonet] Fire in the lab To: Histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 hey, I once set my hair on fire in the lab...singed off my eyebrows and burnt my nostril hair. It took several days for the burnt hair smell to get out of my nose!!. This too was in a time long long ago in another time dimension On Sat, Feb 27, 2010 at 12:51 AM, Joe Nocito wrote: > Once upon a time in a far away land, we used to boil our embedding molds in > boiling soapy water, over an open Bunsen burner, followed by an alcohol > rinse then air dry. One time the fire alarm was activated and we had to > evacuate the hospital. We were out there quit awhile. When we received the > all clear to go back into the hospital, I was the first one back in the lab > and the fire department was there, looking into our pot that had boiled out > and was smoking up the lab. This wasn't the cause of the first alarm, but it > did set off the second. > > Joe > ----- Original Message ----- From: "CHRISTIE GOWAN" > > > To: > Sent: Friday, February 26, 2010 8:20 AM > > Subject: [Histonet] Fire in the lab > > > > > >? Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old paraffin > pot. One of my techs came in on Sunday to embed some tissues, went into the > processor room and smelled something burning. He noticed our old paraffin > pot had charred looking labels on the outside so he went over, opened the > lid and poof!!! the pot went up in flames. The thermostat had gone haywire > and heated the paraffin to flash point. Opening the lid gave it the oxygen > it needed to ignite. He triggered the alarm, made the appropriate call and > then put it out with an extinguisher. Of course it kept re-igniting because > he could not get behind it to pull the plug. The fire dept finally was able > to get it pulled out and unplugged. Needless to say the tech was shaken and > the room was a mess. I applaud his courage and am not sure I would have done > the same. There was enough xylene and alcohol on the 4 processors to cause > quite an explosion but everything else was in a flammable cabinet. I was > wondering if this type of thing had ever happened to anyone else?? Needless > to say, we have de-comissioned all old paraffin pots and will order only > those with over temp safety features. I guess I just wanted to remind > everyone that fires can happen in the lab and do probably more often than we > hear about. This was the first time for me and I have been in this business > for over 20 years. Take care and be safe. > > Christie Gowan HT (ASCP) >? ? ? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 2 Date: Sat, 27 Feb 2010 12:47:01 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW:? [Histonet] ruo antibodies To: "'Vickroy, Jim'" , ??? Message-ID: <5E6E0AB0ED28430797B6300065C77E6D@prueggihctechlt> Content-Type: text/plain;??? charset="us-ascii" Yea we were just looking at this yesterday.? There is no question on the CAP check list about using RUO's anymore, but it is still in the discussion section with ASR's and it is stated as you mentioned to include a disclaimer and statement that you did try to find an IVD or ASR antibody.? Our question was about billing the patient for RUO use.? CAP does not really address billing issues like that, but CMS does as far as we could tell, and we have been told that if you bill for RUO's CMS could come back and deny payment of the claim and perhaps all bills from your lab. We were wondering if anyone is billing patients for RUO antibodies, but of course if you are you may not want to say so to bring attention to yourself. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Friday, February 26, 2010 10:03 AM To: Histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] ruo antibodies Our new CAP checklist does not mention the use of RUO antibodies anymore. This was under the question using ASR antibodies in the past.???I believe the requirement was that if we wanted to use an RUO antibody we had to have a disclaimer similar to the ASR disclaimer but we also had to have a statement stating that we had searched for an IVD or ASR antibody.? Does anyone know if this is still the practice or am I missing something????I do know of course than when using either an ASR or RUO antibody we have to establish and verify the performance.???Any thoughts about the RUO(s)? James Vickroy BS, HT(ASCP) Surgical? and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sat, 27 Feb 2010 12:30:35 -0800 (PST) From: Jeffrey Silverman Subject: [Histonet] Fire in the lab To: histonet@lists.utsouthwestern.edu Message-ID: <442926.94178.qm@web111115.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Here's mine. Had a per diem tech who?didn't like the electric forceps warmer.? She worked over Saturdays alone and brought in an alcohol lamp to heat her forceps and then knocked it over spilling flaming alcohol all over the thermal and dispensing consoles. Then, even worse, she poured water all over both plugged- in electic appliances. We got away with just melted facade?face plates on both instruments. ? Jeff Silverman ------------------------------ Message: 4 Date: Sat, 27 Feb 2010 22:12:50 -0500 From: "Pam Barker" Subject: [Histonet] RELIA Histology Careers Bulletin 2/28/10 Are you ??? into??? Facebook? MySpace? Twitter? or Linkedin? To: "'Histonet'" Message-ID: Content-Type: text/plain;??? charset="us-ascii" Hi Histonetters!! Facebook, Myspace, Linkedin, Twitter;? Have you joined the Social Networking Craze? If so then you know how much fun it can be reconnecting with old friends, making new ones.? If you do have an account on one of these networks I would love to connect with you.? Please shoot me back an e-mail with your id on the site or an invitation to connect/friend/follow and I will respond right away. If that was like a foreign language to you and you would like some help using any of these sites please let me know.? I would be happy to help you join in. I also wanted to tell you about my current job openings.? All of these jobs are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance.? Need help with your resume or following up after the interview or negotiating salary?? Give me a call or shoot me an e-mail.? I would be happy to help out.? This is a complimentary service of RELIA of course. Here is a list of my current open positions HISTOLOGY/PATHOLOGY? MANAGEMENT NY- Orange/Rockland County Histology Supervisor MA - Cape Cod Pathology Supervisor OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA - Central CA - Pathology Supervisor HISTOTECHS FL-Miami Histotechnologist needed for growing private lab GA-Grossing Histotechnologist Night Shift Great Shift diff MA-Boston - Immunohistochemistry Specialist MA-Boston - Histotechs day and evening shift NY-Orange/Rockland County Brand New Lab NYS license or Elig 2nd shift. CA-Los Angeles Histotechnologist afternoon shift PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC - Charlotte PA grad from NAACLES program required FL- Miami Growing private lab MARKETING AND SALES Marketing Rep - Histo/Cyto Products Pacific NW Region CYTOLOGY PA-Pittsburgh Cytology Supervisor If you or any of your friends would like more information on any of the positions listed or help with a job search in another area please contact me.? I would be more than happy to assist you.? You can reach me at 866-607-3542 or? relia1@earthlink.net Remember it never hurts to look. Hope to hear from you soon. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? ? (407)353-5070 FAX:? ? (407)678-2788 Toll Free: (866)607-3542 e-mail:? relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 39 ****************************************