[Histonet] Topic 5: Tissue Processor Selection
Maglione, Marilou (Muscato)
mmaglion <@t> unch.unc.edu
Tue Dec 28 12:52:48 CST 2010
Hi Histonetters,
I thought I'd weigh in on the question about tissue processors...I've been a 'lurker' and have enjoyed everyone's helpful posts. Thanks!
We have had VIP's for many years, with dependable service. But through our efforts to make our lab Lean and more efficient, we've added 3 Peloris processors over the past 2 years. The tissue quality is excellent and the processing times are less than half that of our VIP's. We process fatty tissues in 5.5 hours now and most rushes in 45 min. Each Peloris can function as two separate processors, so for a small lab one retort could be used for rushes while a full-length program is running in the other. We also use less reagent because the Peloris tells us when to change the solutions, helping us avoid extreme variations in reagent quality and wasting reagents that are still good. Our pathologists have been very happy with the quality and the TAT and everyone finds it really easy to use.
Marilou Maglione CT (ASCP), HTL
UNC Healthcare
Chapel Hill, NC
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, December 28, 2010 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 85, Issue 26
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Today's Topics:
1. RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding
procedure manual - Email found in subject (Kim.Donadio <@t> bhcpns.org)
2. Chuck Churukian's Address (Akemi Allison)
3. Use of ammonia water (Sharon.Davis-Devine)
4. What New Tissue Processor Should we get ? (Parker, Helayne)
5. RE: What New Tissue Processor Should we get ? (Nails, Felton)
6. Listing (M.N.)
7. RE: Use of ammonia water (Susan.Walzer <@t> HCAHealthcare.com)
8. RE: Use of ammonia water (Bartlett, Jeanine (CDC/OID/NCEZID))
9. RE: MPB antibody (Settembre, Dana)
10. bouin solution (wassan alkadhumi)
11. AW: [Histonet] bouin solution (Gudrun Lang)
12. Re: Use of ammonia water (Victoria Baker)
----------------------------------------------------------------------
Message: 1
Date: Mon, 27 Dec 2010 14:28:24 -0600
From: Kim.Donadio <@t> bhcpns.org
Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding
procedure manual - Email found in subject
To: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF15A439EE.16C8EA1C-ON86257806.00702344-86257806.007076F0 <@t> bhcpns.org>
Content-Type: text/plain; charset="US-ASCII"
Do others document separately the original creation, training, competency,
implementation of procedures/processes and then continual yearly training,
competency and review of those processes? Are you using a Quality
Management System? How about Electronic system to manage?
Yes, to all of those. We document original training and then all
competencies are part of their yearly eval. We also have the QMS
electronic policy/procedure Data base that sends each individual reminders
when they need to read a update or sign off on the yearly requirement that
they have read and understand the material.
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996
WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/23/2010 10:49 AM
To
<rcazares <@t> schosp.org>, histonet <histonet <@t> lists.utsouthwestern.edu>
cc
Subject
RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual -
Email found in subject
Thanks, I am aware of Peggy's form. We have similar hard copy forms now,
but we are moving to an electronic system. It will be much more manageable
for 125 employees.
When you state in your form the employee is competent, do you have
documentation to support that there was a competency evaluation? We only
state the employee has read and understands. We have separate
documentation for training and competency.
I love this type of discussion string. There are so many different
processes out in Histoland.
Do others document separately the original creation, training, competency,
implementation of procedures/processes and then continual yearly training,
competency and review of those processes? Are you using a Quality
Management System? How about Electronic system to manage?
William DeSalvo, B.S., HTL(ASCP)
> From: RCazares <@t> schosp.org
> To: wdesalvo.cac <@t> hotmail.com
> Date: Thu, 23 Dec 2010 10:17:48 -0600
> Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure
manual - Email found in subject
>
> Hello histonetters,
>
> When I implement a new procedure in my lab, besides the routine yearly
sign-off sheet that accompanies the procedure, there an additional sheet
that states
>
> " The following employees have reviewed this procedure and by signing
below are acknowledging that they understand and are competent in
performing the procedure:"
>
> Below that statement is a box with signature and date lines for all the
employees using this procedure to sign. This makes each employee
responsible for reading and understanding the procedure. This I got from
one of Peggy Wenk's workshops (Thanks Peggy!!). I can send a copy to
anyone who'd like one, just contact me directly.
>
>
> Ruth Cazares, HT (ASCP)
> Histology Supervisor
> Department of Pathology
> Swedish Covenant Hospital
> Chicago, IL
>
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
> Sent: Thursday, December 23, 2010 7:27 AM
> To: bsullivan <@t> shorememorial.org; Victor Tobias
> Cc: histonet; histonet-bounces <@t> lists.utsouthwestern.edu
> Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure
manual - Email found in subject
>
>
> Beatrice,
> How do you show proof to CAP that your employees that use the procedures
and perform the tasks described in the procedures have reviewed and
understand the procedure when there is a new one during your cycle year
and before it is implemented?
>
> CAP has changed the way they inspect and they are now looking for how
whether the employees performing a task described in the procedure
understands the procedure and is performing exactly as the procedure
describes. They are not as concerned that the Supervisor and management
knows about the procedures, they want to see how informed, trained and
competent the bench employee is.
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>
>
> > To: victor <@t> pathology.washington.edu
> > From: BSullivan <@t> shorememorial.org
> > Date: Thu, 23 Dec 2010 07:53:22 -0500
> > Subject: Re: [Histonet] CAP Question regarding procedure manual
> > CC: Histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
> >
> > Victor,
> > To my knowledge all you need is proof that the staff reviewed the
manuals.
> > We accomplish this by a sign off sheet in the front of each manual we
use.
> > The Supervisor, or designee, needs to review and sign off on each
procedure
> > in each manual.
> >
> > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
> > AP Supervisor
> > Shore Memorial Hospital
> > 609-653-3590
> >
> >
> > Speak only well of people and you need never whisper
> >
> >
> >
> > Victor Tobias
> > <victor <@t> pathology
> > .washington.edu> To
> > Sent by: Histonet
> > histonet-bounces@ <Histonet <@t> lists.utsouthwestern.edu>
> > lists.utsouthwest cc
> > ern.edu
> > Subject
> > [Histonet] CAP Question regarding
> > 12/22/2010 06:16 procedure manual
> > PM
> >
> >
> >
> >
> >
> >
> >
> >
> >
> > Is there a requirement to have a signature of every staff member on a
> > procedure if they perform that procedure in a manual? Wouldn't one
> > signature on a cover page suffice that you have read and understand
what
> > is in the manual?
> >
> > Victor
> >
> > --
> > Victor Tobias
> > Clinical Applications Analyst
> > University of Washington Medical Center
> > Dept of Pathology Room BB220
> > 1959 NE Pacific
> > Seattle, WA 98195
> > victor <@t> pathology.washington.edu
> > 206-598-2792
> > 206-598-7659 Fax
> > =================================================
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> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > _______________________________________________
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> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
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Message: 2
Date: Mon, 27 Dec 2010 12:57:31 -0800 (PST)
From: Akemi Allison <akemiat3377 <@t> yahoo.com>
Subject: [Histonet] Chuck Churukian's Address
To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <563441.88990.qm <@t> web113805.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi All,
I just spoke with Chuck a few minutes ago and his spirits are up. He is working
with a physical therapist this afternoon. He gave me permission to post his
address, and would love to hear from those of you who would like to send him
cards. I think it would really cheer him up.
His address is:
34 Gailhaven Court
Rochester, NY 14618
Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3377 <@t> yahoo.com
------------------------------
Message: 3
Date: Mon, 27 Dec 2010 14:58:20 -0600
From: Sharon.Davis-Devine <Sharon.Davis-Devine <@t> carle.com>
Subject: [Histonet] Use of ammonia water
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE4513DAAA85804D9A9FBAC9C1EE7A668EDBEE75 <@t> EXCHANGECCABE.carle.com>
Content-Type: text/plain; charset="us-ascii"
Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks.
Sharon Davis-Devine, CT (ASCP)
Cytology-Histology Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
sharon.davis-devine <@t> carle.com
------------------------------
Message: 4
Date: Mon, 27 Dec 2010 15:15:44 -0600
From: "Parker, Helayne" <HParker <@t> Skaggs.Net>
Subject: [Histonet] What New Tissue Processor Should we get ?
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<930EB2E8DF68C544873EDD2A3D5F506004700E6898 <@t> email1.skaggs.net>
Content-Type: text/plain; charset="us-ascii"
Hi All,
If given the choice- what would be the best Tissue Processor to get for a small Path Lab. We have always had VIPs. Ours is about 16 y/o and we need to replace it and use it as a back up or for small biopsies. I was looking at the new VIPs and our medical repair people are very happy w/ the VIPs in the past. I am looking at VIP 5 and 6. Do not need anything too fancy. Just want a workhorse that has little or no issues and is user friendly.
Any input is much appreciate on these or other products - private emails great !
Thanks,
Helayne Parker
hparker <@t> skaggs.net
CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you.
------------------------------
Message: 5
Date: Mon, 27 Dec 2010 15:19:37 -0600
From: "Nails, Felton" <flnails <@t> texaschildrens.org>
Subject: [Histonet] RE: What New Tissue Processor Should we get ?
To: "'Parker, Helayne'" <HParker <@t> Skaggs.Net>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C1FE5960057C084CA389CE977790629090652BDB <@t> TCDMSG01.ad.TexasChildrensHospital.org>
Content-Type: text/plain; charset=us-ascii
As have you, I have also had great service from my VIP's and plan on purchasing the VIP 6.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Parker, Helayne
Sent: Monday, December 27, 2010 3:16 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] What New Tissue Processor Should we get ?
Hi All,
If given the choice- what would be the best Tissue Processor to get for a small Path Lab. We have always had VIPs. Ours is about 16 y/o and we need to replace it and use it as a back up or for small biopsies. I was looking at the new VIPs and our medical repair people are very happy w/ the VIPs in the past. I am looking at VIP 5 and 6. Do not need anything too fancy. Just want a workhorse that has little or no issues and is user friendly.
Any input is much appreciate on these or other products - private emails great !
Thanks,
Helayne Parker
hparker <@t> skaggs.net
CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you.
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------------------------------
Message: 6
Date: Mon, 27 Dec 2010 22:04:46 -0800
From: "M.N." <mnglegal <@t> gmail.com>
Subject: [Histonet] Listing
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTinK1Sosw5ScH7KNHL7ajFxMVep__S5Dv1h0UiZD <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi - I am looking for someone in the LA area (ideally someone who works in a
lab or hospital setting) to teach me how to process biopsies from start to
finish, and to cut paraffin-embedded sections. I would compensate you for
your time, of course. Please contact me with your availability - I'd like
to get started soon. Thanks!
------------------------------
Message: 7
Date: Tue, 28 Dec 2010 01:57:56 -0600
From: <Susan.Walzer <@t> HCAHealthcare.com>
Subject: [Histonet] RE: Use of ammonia water
To: <Sharon.Davis-Devine <@t> carle.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4BF03F5404EBDE409AF9232DA74B9DED2B3D09D7CA <@t> FWDCWPMSGCMS09.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
We have always used water with liquid soap and some ammonia to soften blocks. It has had no effect on IHC.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine
Sent: Monday, December 27, 2010 3:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Use of ammonia water
Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks.
Sharon Davis-Devine, CT (ASCP)
Cytology-Histology Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
sharon.davis-devine <@t> carle.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 28 Dec 2010 05:51:55 -0500
From: "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] Use of ammonia water
To: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<E129883C1CDAD2438D2680E08E0C2A0DAFC306 <@t> LTA3VS022.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii
I use it on particularly difficult blocks and have seen no ill effects.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sharon.Davis-Devine
Sent: Monday, December 27, 2010 3:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Use of ammonia water
Happy Holidays Histonetters, I have a question for you. Do any of you
use ammonia water to soften the blocks before cutting? If so, does this
have any effect on IHC staining? Any help will be greatly appreciated.
Thanks.
Sharon Davis-Devine, CT (ASCP)
Cytology-Histology Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
sharon.davis-devine <@t> carle.com
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Tue, 28 Dec 2010 09:40:19 -0500
From: "Settembre, Dana" <settembr <@t> umdnj.edu>
Subject: [Histonet] RE: MPB antibody
To: "'Taylor, Jean'" <jtaylor <@t> meriter.com>, "'ihcrg <@t> googlegroups.com'"
<ihcrg <@t> googlegroups.com>, "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B64B947688FB794A8C191D19F22927C875BE700A <@t> UMDEXMBX02.core.umdnj.edu>
Content-Type: text/plain; charset="us-ascii"
Hi Jean,
I use Leica's MBP which the get from NovoCastra cat. NCL-MBP
I use it on formalin fixed paraffin embedded human tissue on my Dako.
I use Dako's Target Ret. Soln in a steamer for 40 minutes
Incubate 15 min with it at the dilution of 1:25.
Then I use Dako LSAB2 kit.
It works fine.
Good luck,
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taylor, Jean
Sent: Thursday, December 23, 2010 2:14 PM
To: 'ihcrg <@t> googlegroups.com'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] MPB antibody
Hi Everyone,
Has anyone worked with the MBP (human eosinophilic major basic protein) antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation.
Thanks,
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
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------------------------------
Message: 10
Date: Tue, 28 Dec 2010 06:49:54 -0800 (PST)
From: wassan alkadhumi <w_alkadhumi <@t> yahoo.com>
Subject: [Histonet] bouin solution
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <604286.3701.qm <@t> web45206.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear Histonet
I have question concerning bouin solution, we use it in masson trichrome before
starting the stain. we incubate the slides trimmed at 3 micron in bouin solution
for 18 hr in room temp which is not controlled. Then we wash the slides using
running water first and then distilled water, but according to what
i red, when using bouin solution,the tissue should be washed with (50-70)%
alcohol to prevent deterioration of staining in time. now i should mention that
in the our masson trichrome procedure there is no washing with alcohol but only
with water.
what should i do? In the book its mentioned that we have to wash with alcohol if
the tissue is not processed yet, is that why the alcohol step is missing in
masson tri procedure?and why will the stain deteriorate if some of the bouin
solution stayed in the tissue?
The main fixative that is used in our lab for all grooses and biopsy is 10%
formalin (except bone marrow biopsy). bouin solution is used after processing of
the tissue and deparaffinization of the sections is finished.
Thanks
Wassan
Histotechnician
Shorh hospital
North of Iraq
------------------------------
Message: 11
Date: Tue, 28 Dec 2010 17:42:52 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] bouin solution
To: "'wassan alkadhumi'" <w_alkadhumi <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2CFC6E180EFB469AA061237DC32D690B <@t> dielangs.at>
Content-Type: text/plain; charset="iso-8859-1"
We incubate the trichrome-slides after deparaffination for 2 hours in 56°C,
then rinse in warm tapwater until the slides are colourless. Then another
rinse in distilled water, because I don't want carry over from tapwater into
the weigert's iron hematoxylin. ...
If you let bouins in the tissue, it acts like a stain. Think of vanGieson
staining solution (also called picrofuchsin).
It sounds like the processing after bouin-fixation and the trichrom staining
are mixed up. Or the protocols of trichrom-staining of NBF-fixed and
bouin-fixed tissue are mixed up.
Gudrun
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von wassan
alkadhumi
Gesendet: Dienstag, 28. Dezember 2010 15:50
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] bouin solution
Dear Histonet
I have question concerning bouin solution, we use it in masson trichrome
before
starting the stain. we incubate the slides trimmed at 3 micron in bouin
solution
for 18 hr in room temp which is not controlled. Then we wash the
slides using
running water first and then distilled water, but according to what
i red, when using bouin solution,the tissue should be washed with (50-70)%
alcohol to prevent deterioration of staining in time. now i should
mention that
in the our masson trichrome procedure there is no washing with alcohol but
only
with water.
what should i do? In the book its mentioned that we have to wash with
alcohol if
the tissue is not processed yet, is that why the alcohol step is missing in
masson tri procedure?and why will the stain deteriorate if some of the bouin
solution stayed in the tissue?
The main fixative that is used in our lab for all grooses and biopsy is 10%
formalin (except bone marrow biopsy). bouin solution is used after
processing of
the tissue and deparaffinization of the sections is finished.
Thanks
Wassan
Histotechnician
Shorh hospital
North of Iraq
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Message: 12
Date: Tue, 28 Dec 2010 12:47:13 -0500
From: Victoria Baker <bakevictoria <@t> gmail.com>
Subject: Re: [Histonet] Use of ammonia water
To: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<AANLkTimche31m+13x0BUSNN+j_7UkHUMZGCHgLk1DSGM <@t> mail.gmail.com>
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Hi Sharon,
I use a 1.5% solution for bone marrow cases and bloody tissue. It does also
work well for GI. I've never had an issue with it affecting any of my IHC.
An alternative though (when I can't get my hands on ammonium hydroxide) is
to put these blocks in warm water (I'm careful about the waterbath because
of X-contamination issues) for about 30-45 seconds and this works just as
well. I stopped using any surface decal solution or Nair as I did see some
differences with the expression of certain markers - I did not see any false
negs, but the staining itself was not as intense it looked like.
Vikki
On Mon, Dec 27, 2010 at 3:58 PM, Sharon.Davis-Devine <
Sharon.Davis-Devine <@t> carle.com> wrote:
> Happy Holidays Histonetters, I have a question for you. Do any of you use
> ammonia water to soften the blocks before cutting? If so, does this have any
> effect on IHC staining? Any help will be greatly appreciated. Thanks.
>
> Sharon Davis-Devine, CT (ASCP)
> Cytology-Histology Supervisor
> Carle Foundation Hospital
> Laboratory and Pathology Services
> 611 West Park Street
> Urbana, Illinois 61801
> 217-383-3572
> sharon.davis-devine <@t> carle.com
>
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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End of Histonet Digest, Vol 85, Issue 26
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