[Histonet] RE: Parathyroid staining

[Tony Henwood]

Dorothy,

A 0.5% aqueous Toluidine blue will suffice.
Stain either air-dried or alcohol fixed frozen sections for 1-2 minutes, rinse in water, dehydrate quickly, clear and mount.
That's it!

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L
Sent: Friday, 24 December 2010 4:08 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Parathyroid staining

Does anyone use Toluidine blue to stain parathyroids when they come in fresh for a frozen section?  One of our pathologist heard of this and I am not familiar with it.  I have heard of it with the Paragon stain, but not certain what percentage would be needed if only Toluidine blue was used.

Thanks ahead of time and Happy Holidays!!

Dorothy Webb, HT



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