[Histonet] RE: Edge affect on TMA?

[Helen Fedor]

Here is a hint to keep your TMA from falling apart. After construction put the block face down on a glass slide a put in a 37degree C oven for 15-30 minutes (the paraffin should not be allowed to melt). Then take the block/slide out and gently compress with even pressure. (uneven pressure will cause the block to become misshapen). Cool the block at room temperature. You can repeat this process several times to help the cores adhere to the recipient block. The larger the core size, the more severe this problem is. One of the reasons that we choose not to use the 2.0 mm core is that this problem is more severe with this size core.

Hope that will help you.

Helen L. Fedor

Tissue Microarray Lab, Manager
Prostate Spore Lab, Manager
Johns Hopkins University
600 N. Wolfe St, | Marburg Room 406
Baltimore, MD | 21287-7065

410.614.1660





From: IRENA SREBOTNIK KIRBIS [mailto:irena.kirbis <@t> hotmail.com]
Sent: Wednesday, December 22, 2010 1:54 AM
To: Helen Fedor; settembr <@t> umdnj.edu; ahutton <@t> dh.org; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Edge affect on TMA?

how to prevent spots coming off during sectioning TM's? we do sometimes facing problems sectioning TM's, they're just falling apart?
thanks for any hints
Irena

> From: hfedor <@t> jhmi.edu
> To: settembr <@t> umdnj.edu; AHutton <@t> dh.org; histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 21 Dec 2010 16:13:31 -0500
> CC:
> Subject: [Histonet] RE: Edge affect on TMA?
>
> Hello, We do not usually see that. If we do its very rare because I can't think of any examples off hand. The usual problems are the spots came off or shifted/smushed, not an edge effect.
>
> Helen
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Settembre, Dana
> Sent: Tuesday, December 21, 2010 3:00 PM
> To: 'Hutton, Allison'; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Edge affect on TMA?
>
> Does anyone experience edge affect on TMA?
> Dana Settembre
>
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