[Histonet] paraformaldehyde vapour

[Yak-Nam Wang]

Hello,

In a protocol to stain mucin in oesophageal tissue, it calls for "post-fix
in paraformaldehyde vapour at 37 deg C". The protocol is a modified Alcian
blue/PAS staining technique. Can someone share insight into how to fix in
paraformaldehyde vapour? That is, how much paraformaldehye powder is needed
per container area/# of slides. Do I just place powder at the bottom of a
dish with the slides and place in at 37 deg C?

Thank you for your help
Yak-Nam Wang


University of Washington
APL
Seattle, WA 98105