[Histonet] need ur help

[wassan alkadhumi]

Hi all
Hope u r all doing well, am an Iraqi histotech working in both 
histopathology and IHC department in Iraq. One part of what i do is IHC 
(manual) i have no problem with, the other part is what i have problems with, 
Renals and Livers, we do regular H &E stain , PAS and Masson Tirchrome and all 
of them were text book quality until ten days ago, the problem is the Stains 
even the H&E are not Fine, its smugish. And the most important stain  for the 
histopathologist is the Trichrome which it should be fine and delacate and its 
not. I checked the procedures and stain preparations and i doing it the 
correctly , I made new solutions and did prolonged deparaffinization 10 min each 
step (3 xylenes,  2 100%ETOH,  one 90%ETOH, one 70%ETOH) and there was no 
difference. I should mention that those problems happened with only Renal and 
Liver biopsies the H&E and PAS on other types of tissues are working just 
great. the one thing that changed before 10 days ago is the wether it became 
really cold and our lab temp. is not controlled.  Am going crazy please help me
Thank u a lot 

Wassan
Histotech.
Shorsh hospital