[Histonet] Decalcification

[Liz Chlipala]

Try surface decaling the samples in the block.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sherwood, Margaret 
Sent: Wednesday, December 08, 2010 11:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Decalcification

We have small arteries that were FFPE and sectioned that should have
been
decacified before processing.  (The investigator did not indicate as
such when
submitting them).  Cutting was extremely difficult and the sections, as
you can
imagine, had terrible knife marks and chatter.  Of course, they are
important
(for a paper)!  

Is there any way tissue, that has been embedded, can be decalcified at
this
point?  Or some other way to treat the block so that we can get better
sections?

Thanks for all your help.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org 




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