[Histonet] RE: positive slides

[Clare Thornton]

We tried the Surgipath slides, unfortunately, they led to even more inconsistent staining than the superfrost plus.  We haven't had any issues with the superfrost plus slides since early October.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (HorizonNB)
Sent: Tuesday, December 07, 2010 8:25 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: positive slides 

 

The VWR slides (superfrost catalogue #48311-703) are the only slide that is validated to be used on the Ventana platforms.  The problem with these slides is that they are hydrophobic.  They do hold onto the tissue really well, but when you dip them in a water bath or pipette fluid on them while they are sitting on the stainer, you will notice that the fluid pools and does not spread evenly over the entire slide.  This is causing problems with consistency in staining.  Another site in my area has had problems with their FISH staining as a result of these slides as well.  I would like to be able to tell you that Ventana has been able to provide an alternative for this problem, but I can't. *sigh*

Now, if you're ambitious and have loads of time on your hands (sarcasm intended!), Surgipath does produce a charged slide that is hydrophilic.
They are called Apex Superior Adhesive Slides.  My rep sent me a couple of boxes to try, but with our volume of IHC staining and only one instrument, we just haven't been able to squeeze in a validation study.
If you try to do this, please let me know how you make out!

Good luck!

Randi Hayes
Histology Supervisor
Horizon Health Network - Moncton Zone
Moncton, NB  Canada
randi.hayes <@t> HorizoNB.ca
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