[Histonet] immersion fixation and perfusion fixation

[ANJUM PARKAR]

Hi all,
Does anyone have a protocol for whole rat brain immersion fixation using 4%
Paraformaldehyde for IHC where no transcardial perfusion has been performed? I
am specifically interested in knowing how long does the brain need to be
immersed for a good complete fixation before transferring to sucrose. Also, I
would like a protocol for rat brain tissue transcardial perfusion fixation down
to freezing to sectioning if possible to compare with my current protocol.
Thanks!