[Histonet] Small biopsy specimens

[Sebree Linda A]

We've always only used hematoxylin where ever I've worked.  I haven't
embedded in eons so can't tell you how visible those specks of tissue
are. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sharon.Davis-Devine
Sent: Wednesday, December 01, 2010 12:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Small biopsy specimens

We have been using eosin to stain our small biopsies prior to tissue
processing so that they are easier to see when embedding.  Recently one
of our PA's started using Hematoxylin instead of the eosin, because that
is what they used where she trained. Upon further investigation the
manufacturers of both of our tissue processors discourage the use of
eosin because it is very hard on the working parts of the machines and
requires more PM's to prevent damage.  So we are considering switching
to using Heme instead.  Do any of you out there use Heme on your biopsy
specimens?  Do your embedders find it easier to see these tiny tissues
when embedding?  Any pros or cons greatly appreciated.  Thanks.

Sharon Davis-Devine, CT (ASCP)
Cytology-Histology  Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
sharon.davis-devine <@t> carle.com

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