[Histonet] Question from one of our researchers

[Adam .]

While not a cell membrane marker, you can often use differential
interference contrast (DIC) microscopy. The microscope I use has a special
setting that lets you add DIC without any additional staining. In the mouse
bone marrow paraffin sections, it lets you see most edges of most cells.

Adam

On Wed, Dec 1, 2010 at 11:19 AM, Johnson, Teri <TJJ <@t> stowers.org> wrote:

> Can someone give me ideas to pass along to one of our researchers? Of
> course adhesion molecule antibodies are the first thought, but not for
> heterogeneous cell populations. So I was wondering maybe an antibody
> cocktail? Are there any lectins that might show this? Thanks!
>
> "I am wondering what kind of cell membrane marker is recommended to stain
> tissue section for imaging (going to process the image with 3D Imaris
> software).
> Cells are mouse tissue section in paraffin and very heterogeneous. To see
> cell-cell boundary clearly."
>
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