From Kari.Breal <@t> alexian.net Wed Dec 1 07:11:09 2010 From: Kari.Breal <@t> alexian.net (Breal, Kari) Date: Wed Dec 1 07:11:48 2010 Subject: [Histonet] Mohs H&E QC Message-ID: What is the easiest way to be compliant with the CLIA guideline of a daily H&E positive and negative control slide in a Mohs lab? Would a slide with a known positive and a known negative precut and stored in the freezer, then one run with the first case be acceptable? Anyone performing Mohs, please let me know how you are handling this. Thanks, Kari CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mwgfrindall <@t> gmail.com Wed Dec 1 07:51:17 2010 From: mwgfrindall <@t> gmail.com (Matthew Frindall) Date: Wed Dec 1 07:51:20 2010 Subject: [Histonet] used equipment Message-ID: Hello All, I'm an amateur microscopist, mostly interested in botanical specimens. I recently acquired an American Optical A820 microtome. It appears to be in good working condition but is missing the blade. I am considering purchasing a used blade or a used disposable blade holder from Ebay or a lab surplus website. I realise the condition of this material may not be optimal and unfortunately the sellers generally do not know the history of these items. My question is - should I be worried about safety? If there is a chance this material came from pathology labs, do I need worry about any potential prions/viruses etc? Thanks in advance, Matthew From jshelley <@t> sanfordburnham.org Wed Dec 1 07:54:31 2010 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Wed Dec 1 07:54:37 2010 Subject: [Histonet] RE: Mohs H&E QC In-Reply-To: References: Message-ID: Hi Kari, I worked doing Mohs for over 10 years and the way that we handled the issue of a quality control slide is that typically you can find some positive and negative areas within the first patient sample. We would take the very first sample and section for the control slide and run it down to ensure that the stainer was set-up correctly and that the solutions were still viable. If you have any other questions please feel free to contact me. Thanks!!! Kind Regards! ? John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breal, Kari Sent: Wednesday, December 01, 2010 8:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mohs H&E QC What is the easiest way to be compliant with the CLIA guideline of a daily H&E positive and negative control slide in a Mohs lab? Would a slide with a known positive and a known negative precut and stored in the freezer, then one run with the first case be acceptable? Anyone performing Mohs, please let me know how you are handling this. Thanks, Kari CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From king.laurie <@t> marshfieldclinic.org Wed Dec 1 08:01:16 2010 From: king.laurie <@t> marshfieldclinic.org (King, Laurie) Date: Wed Dec 1 08:01:34 2010 Subject: [Histonet] sentinel nodes Message-ID: <201012011401.oB1E1ELH011256@spamfilt> Good morning all, I've been having a strange artifact on sentinel nodes, in only one lab of the two where I perform frozen sectioning services. This artifact does not show up any any other tissue type either. The node is usually almost totally covered with a bubbling, like the tissue is not laying flat. There can be intermittent unaffected areas. These aren't round bubbles, more rectangular and almost orderly in their pattern. I can do nodes in the other lab and they are fine. A colleague here was wondering if the tissue came in saline or some other liquid, which it hasn't. Any ideas? Laurie King HT (ASCP ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From sgoebel <@t> mirnarx.com Wed Dec 1 08:35:17 2010 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Dec 1 08:35:21 2010 Subject: [Histonet] sentinel nodes In-Reply-To: <201012011401.oB1E1ELH011256@spamfilt> References: <201012011401.oB1E1ELH011256@spamfilt> Message-ID: Crystals maybe? How cold is the cryostat? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of King, Laurie Sent: Wednesday, December 01, 2010 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sentinel nodes Good morning all, I've been having a strange artifact on sentinel nodes, in only one lab of the two where I perform frozen sectioning services. This artifact does not show up any any other tissue type either. The node is usually almost totally covered with a bubbling, like the tissue is not laying flat. There can be intermittent unaffected areas. These aren't round bubbles, more rectangular and almost orderly in their pattern. I can do nodes in the other lab and they are fine. A colleague here was wondering if the tissue came in saline or some other liquid, which it hasn't. Any ideas? Laurie King HT (ASCP ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From af46 <@t> buffalo.edu Wed Dec 1 09:13:00 2010 From: af46 <@t> buffalo.edu (Annette Featherstone) Date: Wed Dec 1 09:14:56 2010 Subject: [Histonet] The Milestone PATHOS Message-ID: Has anyone used or is currently using The Milestone PATHOS for tissue processing? What are your results, pros and cons? Would this be good for breast biopsies? Thanks Annette Featherstone From scorpionrider <@t> cox.net Wed Dec 1 09:49:30 2010 From: scorpionrider <@t> cox.net (Mark Turner) Date: Wed Dec 1 09:49:33 2010 Subject: [Histonet] c-erbB2 In-Reply-To: <95927.80782.qm@web82303.mail.mud.yahoo.com> Message-ID: <20101201104930.O23LV.263405.imail@fed1rmwml38> Yes. What's up with that? Mark ---- Cindy Bulmer wrote: > Hello Histoland, > ? > Has anyone seen any "B-9 ducts" staining positive with the c-erbB2 Oncoprotein > (Rabbit, conc.) # A0485 from Dako? > ? > Thank you, > Cindy > > Cynthia Bulmer HT(ASCP)QIHC > IHC Supervisor, CTPL > Waco, TX > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barb.Richmond <@t> avera.org Wed Dec 1 09:55:58 2010 From: Barb.Richmond <@t> avera.org (Barbara Richmond) Date: Wed Dec 1 09:56:30 2010 Subject: [Histonet] Special Stain Automation Message-ID: <0B820C359DEAB540A73BF7A96D0ED80302883E80@ahdc169n1.averamail.net> We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. From trathborne <@t> somerset-healthcare.com Wed Dec 1 10:03:11 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Dec 1 10:03:38 2010 Subject: [Histonet] H. PYLORI STAIN Message-ID: Hi, I was wondering if anyone has used the Poly Scientific "Rapid Staining Method for H. Pylori in Gastric Biopsies" staining kit. Is it easy to use? Is the stain crisp and easy to read? Any feedback would be appreciated. Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From flnails <@t> texaschildrens.org Wed Dec 1 10:06:26 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Dec 1 10:06:42 2010 Subject: [Histonet] RE: Special Stain Automation In-Reply-To: <0B820C359DEAB540A73BF7A96D0ED80302883E80@ahdc169n1.averamail.net> References: <0B820C359DEAB540A73BF7A96D0ED80302883E80@ahdc169n1.averamail.net> Message-ID: The problem with the Nexus is that you can not do silver and AFB on the same run. We use the Dako artisan with very consistent results and it is very easy to use. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From JWeems <@t> sjha.org Wed Dec 1 10:26:57 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Dec 1 10:27:02 2010 Subject: [Histonet] RE: Special Stain Automation In-Reply-To: <0B820C359DEAB540A73BF7A96D0ED80302883E80@ahdc169n1.averamail.net> References: <0B820C359DEAB540A73BF7A96D0ED80302883E80@ahdc169n1.averamail.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044A0FBC88@CHEXCMS10.one.ads.che.org> I recommend the Artisan from DAKO. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 10:56 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sfeher <@t> CMC-NH.ORG Wed Dec 1 10:29:32 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Dec 1 10:29:39 2010 Subject: [Histonet] Special Stain Automation In-Reply-To: <0B820C359DEAB540A73BF7A96D0ED80302883E80@ahdc169n1.averamail.net> References: <0B820C359DEAB540A73BF7A96D0ED80302883E80@ahdc169n1.averamail.net> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC24386955@exchange.cmc-nh.org> Barbara, We are using the Dako Artisan Link for our special stains and love it. We are getting set to interface the Link to our LIS (SoftPath). This will give us the capability to use the barcodes printed on our slides to bring up the pathologist order directly on the Artisan. We also do a fair number of GMS stains on Non-gyn Thin Prep slides for respiratory specimens looking for Pneumocystis. I would recommend getting a demo from the Dako rep. Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Wed Dec 1 11:19:23 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Wed Dec 1 11:19:31 2010 Subject: [Histonet] Question from one of our researchers Message-ID: Can someone give me ideas to pass along to one of our researchers? Of course adhesion molecule antibodies are the first thought, but not for heterogeneous cell populations. So I was wondering maybe an antibody cocktail? Are there any lectins that might show this? Thanks! "I am wondering what kind of cell membrane marker is recommended to stain tissue section for imaging (going to process the image with 3D Imaris software). Cells are mouse tissue section in paraffin and very heterogeneous. To see cell-cell boundary clearly." From anonwums1 <@t> gmail.com Wed Dec 1 11:24:33 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Wed Dec 1 11:24:38 2010 Subject: [Histonet] Question from one of our researchers In-Reply-To: References: Message-ID: While not a cell membrane marker, you can often use differential interference contrast (DIC) microscopy. The microscope I use has a special setting that lets you add DIC without any additional staining. In the mouse bone marrow paraffin sections, it lets you see most edges of most cells. Adam On Wed, Dec 1, 2010 at 11:19 AM, Johnson, Teri wrote: > Can someone give me ideas to pass along to one of our researchers? Of > course adhesion molecule antibodies are the first thought, but not for > heterogeneous cell populations. So I was wondering maybe an antibody > cocktail? Are there any lectins that might show this? Thanks! > > "I am wondering what kind of cell membrane marker is recommended to stain > tissue section for imaging (going to process the image with 3D Imaris > software). > Cells are mouse tissue section in paraffin and very heterogeneous. To see > cell-cell boundary clearly." > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Sharon.Davis-Devine <@t> carle.com Wed Dec 1 12:01:58 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Dec 1 12:02:04 2010 Subject: [Histonet] Small biopsy specimens Message-ID: We have been using eosin to stain our small biopsies prior to tissue processing so that they are easier to see when embedding. Recently one of our PA's started using Hematoxylin instead of the eosin, because that is what they used where she trained. Upon further investigation the manufacturers of both of our tissue processors discourage the use of eosin because it is very hard on the working parts of the machines and requires more PM's to prevent damage. So we are considering switching to using Heme instead. Do any of you out there use Heme on your biopsy specimens? Do your embedders find it easier to see these tiny tissues when embedding? Any pros or cons greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From LSebree <@t> uwhealth.org Wed Dec 1 12:18:36 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Dec 1 12:18:40 2010 Subject: [Histonet] Small biopsy specimens In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E273839A0FB@UWHC-MAIL01.uwhis.hosp.wisc.edu> We've always only used hematoxylin where ever I've worked. I haven't embedded in eons so can't tell you how visible those specks of tissue are. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, December 01, 2010 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Small biopsy specimens We have been using eosin to stain our small biopsies prior to tissue processing so that they are easier to see when embedding. Recently one of our PA's started using Hematoxylin instead of the eosin, because that is what they used where she trained. Upon further investigation the manufacturers of both of our tissue processors discourage the use of eosin because it is very hard on the working parts of the machines and requires more PM's to prevent damage. So we are considering switching to using Heme instead. Do any of you out there use Heme on your biopsy specimens? Do your embedders find it easier to see these tiny tissues when embedding? Any pros or cons greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Wed Dec 1 12:29:36 2010 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Dec 1 12:29:46 2010 Subject: [Histonet] RE: Special Stain Automation In-Reply-To: References: <0B820C359DEAB540A73BF7A96D0ED80302883E80@ahdc169n1.averamail.net> Message-ID: <4CF64DC0.2B7F.00C9.1@geisinger.edu> Just wondering how many people hate the Artisan's plastic well things that you have to clip on the slides?? >>> "Nails, Felton" 12/1/2010 11:06 AM >>> The problem with the Nexus is that you can not do silver and AFB on the same run. We use the Dako artisan with very consistent results and it is very easy to use. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Barb.Richmond <@t> avera.org Wed Dec 1 12:45:07 2010 From: Barb.Richmond <@t> avera.org (Barbara Richmond) Date: Wed Dec 1 12:47:04 2010 Subject: [Histonet] Special stain Automation Message-ID: <0B820C359DEAB540A73BF7A96D0ED80302883E88@ahdc169n1.averamail.net> Does anyone have experience with the Gemini ES from Thermo Shandon? ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. From zodiac29 <@t> comcast.net Wed Dec 1 12:57:14 2010 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Wed Dec 1 12:57:16 2010 Subject: [Histonet] positive slides Message-ID: <2087366044.31612.1291229834319.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Hello, Can anyone recommend a brand of positive slides that are good for IHC. We are having problems with tissue falling off of the slides. Thank You Jenny From Ronald.Houston <@t> nationwidechildrens.org Wed Dec 1 13:18:41 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Dec 1 13:19:34 2010 Subject: [Histonet] RE: Special Stain Automation In-Reply-To: <4CF64DC0.2B7F.00C9.1@geisinger.edu> References: <0B820C359DEAB540A73BF7A96D0ED80302883E80@ahdc169n1.averamail.net> <4CF64DC0.2B7F.00C9.1@geisinger.edu> Message-ID: Don't have a problem with them at all Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, December 01, 2010 1:30 PM To: 'Barbara Richmond'; histonet@lists.utsouthwestern.edu; Felton Nails Subject: [Histonet] RE: Special Stain Automation Just wondering how many people hate the Artisan's plastic well things that you have to clip on the slides?? >>> "Nails, Felton" 12/1/2010 11:06 AM >>> The problem with the Nexus is that you can not do silver and AFB on the same run. We use the Dako artisan with very consistent results and it is very easy to use. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From vjp2105 <@t> columbia.edu Wed Dec 1 13:58:05 2010 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Wed Dec 1 13:58:12 2010 Subject: [Histonet] positive slides In-Reply-To: <2087366044.31612.1291229834319.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: We use VWR Superfrost Slides Cat # 48311-703 and they are great, no lifting sections. On 12/1/10 1:57 PM, "zodiac29@comcast.net" wrote: Hello, Can anyone recommend a brand of positive slides that are good for IHC. We are having problems with tissue falling off of the slides. Thank You Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jfray80 <@t> hotmail.com Wed Dec 1 14:01:19 2010 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Wed Dec 1 14:01:23 2010 Subject: [Histonet] Shurwave Microwave Message-ID: Histonetters , does anyone have a protocol for large polyps 0.5 cm and larger using the TBS Shurwave Microwave Processor. Thanks Histojoe From mhale <@t> carisls.com Wed Dec 1 14:20:09 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Wed Dec 1 14:20:13 2010 Subject: [Histonet] HT Position Message-ID: <6F33D8418806044682A391273399860F0623AA84@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From Cathy.Crumpton <@t> tuality.org Wed Dec 1 14:52:14 2010 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Wed Dec 1 14:52:28 2010 Subject: [Histonet] Part time postition for histology lab assistant Message-ID: We have a part time with benefits histology lab assistant positio open in Hillsboro, OR. Go to [1]www.Tuality.org for more information if interested. W pathologists and handle the acc department. The hours are M-F 12-5 pm. Cathy Crumpton HT(ASCP), Histology Lead Tu Hillsboro, OR 97123 (503)681-1292 < References 1. 3D"http://www.Tuality.org"/ From histopathy <@t> hotmail.com Wed Dec 1 16:19:55 2010 From: histopathy <@t> hotmail.com (histopathy@hotmail.com) Date: Wed Dec 1 16:19:59 2010 Subject: [Histonet] High temp/paraffin oven Message-ID: <620679936-1291241995-cardhu_decombobulator_blackberry.rim.net-1122798500-@bda811.bisx.prod.on.blackberry> Hello to all, Any idea why my middle paraffin oven would be out of range, but the othet two on opposite sides are not? Histopathy Sent on the Sprint? Now Network from my BlackBerry? From Patel.Payal <@t> synthes.com Wed Dec 1 16:20:46 2010 From: Patel.Payal <@t> synthes.com (Patel.Payal@synthes.com) Date: Wed Dec 1 16:20:52 2010 Subject: [Histonet] Technovit 7200 VLC Message-ID: Hello Histonetters! Hope everyone had a nice Thanksgiving. I am using Technovit 7200 VLC kit to embed undecalcified 5-8mm, (varies per sample), thick sections of vertebral samples. If anyone has experience with infiltration times and length of time under yellow and blue light and would not mind sharing that knowledge, I would greatly appreciate it! Thank you, Payal From amosbrooks <@t> gmail.com Wed Dec 1 12:07:40 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Dec 1 17:07:56 2010 Subject: [Histonet] Hellerstrom & Hellman anyone? Message-ID: <201012011307.41020.amosbrooks@gmail.com> Hi, I just did a Hellerstrom & Hellman alcoholic silver stain for pancreatic D- cells. I followed the method described by Bancroft & Stevens. When the slides were placed into the reducing solution they instantly precipitated into a toasty mess, the solution turned milky brown and the slides (even around the sections) picked up a lot of silver precipitate. If anyone has done this of could offer suggestions about it, I'd really like to trouble shoot the stain a bit. A bit of background about the stain that I thought I should add. According to the published method I used a 10% silver solution in ETOH with 100ul of 1M nitric acid. The pH of the solution was 5.0. The reducing solution was alcoholic formalin with 5% pyrogallic acid. I thought the silver solution seemed really concentrated (It hardly even disolved). Incubating such a solution overnight at 37 deg C (as indicated in the method) seemed a bit much. Has anyone heard of modifications of this stain? There is no sense reinventing the wheel, but I certainly don't want to repeat it knowing that it will innevitably do the same thing again unless there is something glaringly obvious that I did wrong. Thanks, Amos From collette2 <@t> llnl.gov Wed Dec 1 18:47:56 2010 From: collette2 <@t> llnl.gov (Collette, Nicole M.) Date: Wed Dec 1 18:49:30 2010 Subject: [Histonet] Question from one of our researchers In-Reply-To: Message-ID: Hi, Teri, How about this? It also gives a brief blurb in the description about commonly used alternatives, if this isn't your thing. I've never tried it, so I can't vouch for it myself, but Invitrogen does make some pretty darn good stuff... http://probes.invitrogen.com/media/pis/mp10045.pdf Sincerely, Nicole Collette Lawrence Livermore National Lab On 12/1/10 9:19 AM, "Johnson, Teri" wrote: > Can someone give me ideas to pass along to one of our researchers? Of course > adhesion molecule antibodies are the first thought, but not for heterogeneous > cell populations. So I was wondering maybe an antibody cocktail? Are there any > lectins that might show this? Thanks! > > "I am wondering what kind of cell membrane marker is recommended to stain > tissue section for imaging (going to process the image with 3D Imaris > software). > Cells are mouse tissue section in paraffin and very heterogeneous. To see > cell-cell boundary clearly." > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Dec 1 20:56:31 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Dec 1 20:57:32 2010 Subject: [Histonet] High temp/paraffin oven References: <620679936-1291241995-cardhu_decombobulator_blackberry.rim.net-1122798500-@bda811.bisx.prod.on.blackberry> Message-ID: Not as much room/air flow for vents to dissipate extra heat? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of histopathy@hotmail.com Sent: Wed 12/1/2010 4:19 PM To: Histonet Subject: [Histonet] High temp/paraffin oven Hello to all, Any idea why my middle paraffin oven would be out of range, but the othet two on opposite sides are not? Histopathy Sent on the Sprint? Now Network from my BlackBerry? From CIngles <@t> uwhealth.org Wed Dec 1 20:58:20 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Dec 1 20:58:51 2010 Subject: [Histonet] Hellerstrom & Hellman anyone? References: <201012011307.41020.amosbrooks@gmail.com> Message-ID: Did you acid clean your glassware before hand? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Amos Brooks Sent: Wed 12/1/2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hellerstrom & Hellman anyone? Hi, I just did a Hellerstrom & Hellman alcoholic silver stain for pancreatic D- cells. I followed the method described by Bancroft & Stevens. When the slides were placed into the reducing solution they instantly precipitated into a toasty mess, the solution turned milky brown and the slides (even around the sections) picked up a lot of silver precipitate. If anyone has done this of could offer suggestions about it, I'd really like to trouble shoot the stain a bit. A bit of background about the stain that I thought I should add. According to the published method I used a 10% silver solution in ETOH with 100ul of 1M nitric acid. The pH of the solution was 5.0. The reducing solution was alcoholic formalin with 5% pyrogallic acid. I thought the silver solution seemed really concentrated (It hardly even disolved). Incubating such a solution overnight at 37 deg C (as indicated in the method) seemed a bit much. Has anyone heard of modifications of this stain? There is no sense reinventing the wheel, but I certainly don't want to repeat it knowing that it will innevitably do the same thing again unless there is something glaringly obvious that I did wrong. Thanks, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Dec 2 05:13:57 2010 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Thu Dec 2 05:16:34 2010 Subject: [Histonet] Special Stain Automation In-Reply-To: <0B820C359DEAB540A73BF7A96D0ED80302883E80@ahdc169n1.averamail.net> References: <0B820C359DEAB540A73BF7A96D0ED80302883E80@ahdc169n1.averamail.net> Message-ID: We get great silvers with our Dako Artisan. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Thu Dec 2 08:20:37 2010 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Thu Dec 2 08:20:48 2010 Subject: [Histonet] Protocol for immunofluorescence staining Message-ID: <5373.203.135.35.66.1291299637.squirrel@brain.net.pk> Dear All, We are looking protocol for immunofluorescence staining Fibrinogen/FITC and positive control, negative control tissue. Muhammad Tahseen SKMCH&RC Lahore,Pakistan From alyssa <@t> alliedsearchpartners.com Thu Dec 2 09:08:07 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Dec 2 09:08:13 2010 Subject: [Histonet] Dermatology Billing Message-ID: Hi Histoland, My apologies if this is a little off subject, but since it has to do with Dermatology I thought I would give it a shot. A large billing company in Casselberry, FL (just outside of Orlando, FL) is looking for a Manager for National Billing Service. I wanted to do them a favor and see if thier are any qualified candidates for this in Histoland. Please feel free to contact me at anytime and pass this along to anyone you might know who is interested. My contact information is below :-) *Manager for National Billing Service (Dermatology)* *Location: Casselberry, Florida (Orlando)* *Employment Type: Full-time* *Salary Range: Commensurate with experience* *Benefits: Health, vision, dental, ST/LT Disability, 401K, Vacation* ? Management experience with billing service or very large medical practice required ? BS degree or five+ years of billing service-related experience required ? Dermatology experience is a plus ? Solid leadership skills with emphasis on team-building and performance accountability ? Strong organizational skills ? Strong financial background ? Customer service oriented geared towards quality and excellence ? Poised for company growth ? Responsibilities will include o Managing approximately 40+ employees o Correspondence and conversations with physicians and medical office staff o Monthly presentations to internal management ? Analyze financial status and trends ? Analyze billing service statistical data ? Audit and evaluate for quality and performance o Short and long-term goal administration o Monthly presentations to clients ? Analyze financial status and trends ? Analyze practice statistical data ? Summarize practice issues, concerns and problem resolution ? Address service quality and employee performance ? Maintain positive and open lines of communication o Weekly meetings with department supervisors ? Accountability for work ? Problem troubleshooting ? Organizational compliance and overview ? Focus on efficiency o Travel required Responsible to CEO and Owner Please let me know if you or anyone you know may be interested. They can contact me with their resume at Alyssa@alliedsearchpartners.com -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From rjbuesa <@t> yahoo.com Thu Dec 2 09:14:32 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 2 09:14:35 2010 Subject: [Histonet] High temp/paraffin oven In-Reply-To: <620679936-1291241995-cardhu_decombobulator_blackberry.rim.net-1122798500-@bda811.bisx.prod.on.blackberry> Message-ID: <318466.43579.qm@web65708.mail.ac4.yahoo.com> That is a thermostat problem. Had it check out. Ren? J. --- On Wed, 12/1/10, histopathy@hotmail.com wrote: From: histopathy@hotmail.com Subject: [Histonet] High temp/paraffin oven To: "Histonet" Date: Wednesday, December 1, 2010, 5:19 PM Hello to all, Any idea why my middle paraffin oven would be out of range, but the othet two on opposite sides are not? Histopathy Sent on the Sprint? Now Network from my BlackBerry? -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu Dec 2 09:41:42 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Dec 2 09:41:47 2010 Subject: [Histonet] Light Green Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E474B8@nmdamailsvr.nmda.ad.nmsu.edu> Why does my light green counter stain just stain the edges of the sections??? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From relia1 <@t> earthlink.net Thu Dec 2 11:49:36 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Dec 2 11:49:35 2010 Subject: [Histonet] RELIA Special Job Alert Histology opportunities in NY Message-ID: Hi Histonetters!! I hope you had a great Thanksgiving. I have several clients that are in need of histo techs and supervisors in New York so I thought I would put the word out. At this point since it is the end of the year my clients are willing to let you call the shots on how you would like to proceed i.e. interview now or after the holidays. Of course these are all permanent full time positions offering excellent salaries, benefits and relocation assistance. * State of NY license or elig.* Here is a list of my current histo tech and supervisory positions: Orange/Rockland County NY - Manager of QA-Private Pathology Lab Rochester, NY - Histotech with Dermpath experience Orange/Rockland County, NY - Day shift Histotechs If you or anyone you know is interested call me toll free at 866-607-3542 or e-mail me at relia1@earthlink.net Thanks and Have a Great Day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From histo20 <@t> hotmail.com Thu Dec 2 12:18:32 2010 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Thu Dec 2 12:18:37 2010 Subject: [Histonet] Competencies Message-ID: When a Histotech leaves their place of employment - does anyone know if their competencies need to be kept for any period of time or can the competencies be disgarded after their departure? Thanks for your help! Paula Wilder St. Joseph Medical Center From christina.thurby <@t> bms.com Thu Dec 2 12:38:23 2010 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Thu Dec 2 12:38:28 2010 Subject: [Histonet] Question about acid phosphatase staining on FFPE and control tissue Message-ID: Hi, We are going to try acid phosphatase staining on FFPE cardiac tissue (I have a couple of papers where this has been performed with success). My question for any of you with expertise in this area is what type of tissue should I use as a Method Development control? Would there be acid phosphatase activity in virtually any normal muscle tissue (ie the epitheloid cell lineage) or possibly just choose prostate? Just curious if any of you out there may have a good suggestion for the control tissue. Thanks, Kristie Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From christina.thurby <@t> bms.com Thu Dec 2 13:26:55 2010 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Thu Dec 2 13:27:01 2010 Subject: [Histonet] RE: Question about acid phosphatase staining on FFPE and control tissue In-Reply-To: <0ff0b30a-6d55-47c6-a216-0034e33ee00a@ushpwbmsmhp002.one.ads.bms.com> References: <0ff0b30a-6d55-47c6-a216-0034e33ee00a@ushpwbmsmhp002.one.ads.bms.com> Message-ID: Hi, We are going to try acid phosphatase staining on FFPE cardiac tissue (I have a couple of papers where this has been performed with success). My question for any of you with expertise in this area is what type of tissue should I use as a Method Development control? Would there be acid phosphatase activity in virtually any normal muscle tissue (ie the epitheloid cell lineage) or possibly just choose prostate? Just curious if any of you out there may have a good suggestion for the control tissue. Thanks, Kristie Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From mhale <@t> carisls.com Thu Dec 2 13:28:13 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Thu Dec 2 13:28:17 2010 Subject: [Histonet] HT Position TN Message-ID: <6F33D8418806044682A391273399860F062846FB@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From mw <@t> personifysearch.com Thu Dec 2 15:33:10 2010 From: mw <@t> personifysearch.com (Matt Ward) Date: Thu Dec 2 15:33:16 2010 Subject: [Histonet] New Histology Field Technical Role Based in AZ/TX/Southern CA Message-ID: <2cf5cbc66f74ecede72ddd7217c9ac46@mail.gmail.com> Good Afternoon Everyone! We have had a world leader open a new Field Technical Histology position that will cover the Pacific Region. The best place to live for this position would be Phoenix, Los Angeles, Or Dallas, and it will focus on Core Histology. This is perfect for a Histotech that is looking for an opportunity to get out of the lab in into the field with a growing company that offers promotion. Please contact me directly by sending me a copy of your resume to mw@personifysearch.com. Thanks! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From dbeil1 <@t> hotmail.com Thu Dec 2 16:14:03 2010 From: dbeil1 <@t> hotmail.com (Damaris Beil) Date: Thu Dec 2 16:14:06 2010 Subject: [Histonet] TBS laboratory equipment Message-ID: Hello everyone, I 'd like to know if anyone is using the TBS the ATP processor, Shur/Stain H&E stainer, and TEC embedding center, manual and automated microtomes. Any feedback would be greatly appreciated. Thanks in advance, Damaris E. Beil, HT, ASCPcm,HTL Associates in Dermatology Histology Laboratory (407) 846-7546 ext. 3307 From jnocito <@t> satx.rr.com Thu Dec 2 16:52:02 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Dec 2 16:52:08 2010 Subject: [Histonet] Hemoglobin A Message-ID: Howdy histoland, does anyone know where to get Hemoglobin A? Dako doesn't have it any more and we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc, etc, etc, Biogenex. No luck. Thanks Joe From raestask <@t> grics.net Thu Dec 2 18:58:44 2010 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Thu Dec 2 18:58:58 2010 Subject: [Histonet] Contact information Message-ID: Could anyone send me the contact information for Charlie Doerner? Rae Ann Staskiewicz From kc <@t> ka-recruiting.com Fri Dec 3 07:37:19 2010 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Fri Dec 3 07:37:25 2010 Subject: [Histonet] Lab Manager Position Message-ID: <842000845.1291383439260.JavaMail.cfservice@SL4APP1> Dear Histonet Community, I am a one of the founders of a healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Laboratory Manager opening at a Dermpath Lab. My client is a Pre-IPO Pathology company who is quickly establishing itself as an industry leader. I'm contacting you to see if you'd be interested in pursuing the Lab Manager position they have open in their Dermpath Lab in Long Island, NY. This state of the art lab s looking for someone with prior management experience to run and manage a lab consisting of 10 FTE's. This Manager will be responsible for overseeing the day to day operations of the lab. My clients offers one of the best compensation & benefits package around including relocation assistance when necessary. For consideration you must have a Bachelor's Degree, be HT or HTL (ASCP) certified and licensed in the State of NY. A minimum of 2 years of prior supervisory or management experience is preferred. If you are interested in learning more about this position, please call or email me at kc@ka-recruiting.com Please contact me if you're interested in learning more about this position or in exploring some of the other numerous job openings we have available across the country. Have a great weekend. KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From mhale <@t> carisls.com Fri Dec 3 09:03:31 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri Dec 3 09:03:37 2010 Subject: [Histonet] Greta HT Position Message-ID: <6F33D8418806044682A391273399860F062DE78D@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From dbeil1 <@t> hotmail.com Fri Dec 3 09:48:04 2010 From: dbeil1 <@t> hotmail.com (Damaris Beil) Date: Fri Dec 3 09:48:10 2010 Subject: [Histonet] TBS Histology Equipment Message-ID: Hello everyone, I was wondering if any of you would be able to give me feedback on the TBS ATP processor, TEC embedding center, SHUR/Stain H&E stainer, microtomes and other piece of instrument from TBS/SHUR. Thank You, Damaris E. Beil, HT, ASCPcm,HTL Associates in Dermatology Histology Laboratory (407) 846-7546 ext. 3307 From dbeil1 <@t> hotmail.com Fri Dec 3 12:30:49 2010 From: dbeil1 <@t> hotmail.com (Damaris Beil) Date: Fri Dec 3 12:30:54 2010 Subject: [Histonet] FW: TBS Histology Equipment In-Reply-To: References: Message-ID: Damaris E. Beil, HT,ASCPcm, HTL Associates in Dermatology Histology Laboratory (407) 846-7546 ext. 3307 From: dbeil1@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: TBS Histology Equipment Date: Fri, 3 Dec 2010 10:48:04 -0500 Hello everyone, I was wondering if any of you would be able to give me feedback on the TBS ATP processor, TEC embedding center, SHUR/Stain H&E stainer, microtomes and other piece of instrument from TBS/SHUR. Thank You, Damaris E. Beil, HT, ASCPcm,HTL Associates in Dermatology Histology Laboratory (407) 846-7546 ext. 3307 From akbitting <@t> geisinger.edu Fri Dec 3 13:02:59 2010 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Dec 3 13:03:08 2010 Subject: [Histonet] Changing solutions on VIP processors. Message-ID: <4CF8F893.2B7F.00C9.1@geisinger.edu> Hello All, I'm involved in a debate with a colleague about how frequently the tissue processors need to be changed. We do roughly 1000 blocks a day on various runs, utilizing 7 tissue processors. About 100 of those are microwave processed, so I'm subtracting those from the equation. So 900 blocks (mixed tissues, no breast) divided by 6 conventional processors is 150 blocks each. I think it's poor reagent management to change a VIP after 150 blocks. Does anyone disagree with me? If these were processing very fatty tissue, I might think differently, but that's not the case. All opinions are welcome. Thanks, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From akemiat3377 <@t> yahoo.com Fri Dec 3 14:26:39 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Dec 3 14:26:43 2010 Subject: [Histonet] Eosin in Tissue Processor Message-ID: <374906.66310.qm@web113814.mail.gq1.yahoo.com> Happy Friday Everyone, I guess I am having a old persons momment!? I forgot how much eosin and what % to put in the last 100% of the Tissue Processor to show-up small transparent bx's.? Can one of you wonderful people tell me please.? I tissue processor is a VIP 5. Thanks a bunch! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com From Allison_Scott <@t> hchd.tmc.edu Fri Dec 3 16:09:36 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Dec 3 16:09:40 2010 Subject: [Histonet] Workload Recording Message-ID: <1872B4A455B7974391609AD8034C79FC026DFD22@LBEXCH01.hchd.local> Hello to all in histoland. Does anyone keep a log of how many blocks that their techs cut. My boss asked me do I keep this type of information. Anyone doing workload recording? Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From akemiat3377 <@t> yahoo.com Fri Dec 3 18:39:12 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Dec 3 18:39:31 2010 Subject: [Histonet] Eosin in Tissue Processor Message-ID: <69EEA8DB-7A28-410D-AA23-C4E4A8877243@yahoo.com> Happy Friday Everyone, I am sending this inquiry again. For some reason this post did not show I my histonet emaiI. I guess I am having a old persons momment! I forgot how much eosin and what % to put in the last 100% of the Tissue Processor to show-up small transparent bx's. Can one of you wonderful people tell me please. The tissue processor is a VIP 5. Thanks a bunch! Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From akemiat3377 <@t> yahoo.com Fri Dec 3 18:53:31 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Dec 3 18:53:36 2010 Subject: [Histonet] Problems with histonet Message-ID: <884837.79871.qm@web113812.mail.gq1.yahoo.com> I just made 2 posts today and they are not showing up. Is any one having problems too? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com From histotech <@t> imagesbyhopper.com Sat Dec 4 07:39:55 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Dec 4 07:39:57 2010 Subject: [Histonet] Test - please disregard In-Reply-To: Message-ID: <6A77F54DB40549CB8EED8A2E81C13DCE@hopperPC> This is a test to see if my email is working properly. Please disregard. Thanks! From axp969 <@t> psu.edu Sun Dec 5 14:31:17 2010 From: axp969 <@t> psu.edu (ANJUM PARKAR) Date: Sun Dec 5 14:31:23 2010 Subject: [Histonet] immersion fixation and perfusion fixation Message-ID: <1291581077l.663766l.0l@psu.edu> Hi all, Does anyone have a protocol for whole rat brain immersion fixation using 4% Paraformaldehyde for IHC where no transcardial perfusion has been performed? I am specifically interested in knowing how long does the brain need to be immersed for a good complete fixation before transferring to sucrose. Also, I would like a protocol for rat brain tissue transcardial perfusion fixation down to freezing to sectioning if possible to compare with my current protocol. Thanks! From alisha <@t> ka-recruiting.com Mon Dec 6 09:35:46 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Mon Dec 6 09:35:38 2010 Subject: [Histonet] Great Histology Supervisor Job in Ohio Message-ID: <830182997.1291649745837.JavaMail.cfservice@sl4app3> Hi Histonet Members, I am currently working on a Histology Supervisor position in Ohio. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From Herrick.James <@t> mayo.edu Mon Dec 6 09:36:57 2010 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Mon Dec 6 09:37:03 2010 Subject: [Histonet] Plastic discoloration Message-ID: <7267A64D75F58241B577876D8A885631038FE39B@msgebe41> Good morning everyone, I just have a quick question in regards to GMA turning white following embedding. Does anyone have any experience on why our plastic is losing its clarity and turning a whitish color? This doesn't seem to have any impact on staining but it would be nice to know the reason. Our polymerization is carried out in a vacuum chamber (nitrogen is used) and BPO is used as the initiator and JB4 as the accelerator. If any on you have any answers, your help would be greatly appreciated. Have a great day!! Jim From sgoebel <@t> mirnarx.com Mon Dec 6 09:48:07 2010 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Dec 6 09:48:11 2010 Subject: [Histonet] Test Message-ID: Just testing. Trying to see if my email is working Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From histopathy <@t> hotmail.com Mon Dec 6 12:53:40 2010 From: histopathy <@t> hotmail.com (Pamela Gholston) Date: Mon Dec 6 12:58:32 2010 Subject: [Histonet] Manual Copper Stain Message-ID: Does anyone have a manual copper stain procedure to share? Thank you,Histopathy From relia1 <@t> earthlink.net Mon Dec 6 13:42:22 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Dec 6 13:42:28 2010 Subject: [Histonet] RELIA Histology Job Alert 12/06/10 Histotech needed in Coastal Texas - Private Pathology Lab ASCP or eligible. Can you help? Message-ID: Hi Histonetters!! I hope everybody is having a great day. I am working with a client on a new position that I wanted to tell you all about. My client is a private pathology lab located in Coastal Texas. This is a dayshift (early morning) full time permanent position. It is M-F with occasional Saturdays. My client offers a competitive pay, benefits and relocation assistance. Requirements: ASCP or elig. 1-2 yrs plus Routine Histology experience (including hand staining) IHC is a plus. This is an immediate opening but my client is willing to consider candidates who prefer to start work after the holidays as well. If you or anyone you know might be interested please contact me - Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. *Thanks for your time and Have a great day!* Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From relia1 <@t> earthlink.net Mon Dec 6 14:16:17 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Dec 6 14:16:20 2010 Subject: [Histonet] RELIA Histology Job Alert 12/06/10 Histotech needed in Coastal Texas - Private Pathology Lab ASCP or eligible. Can you help? Message-ID: Hi Histonetters!! I hope everybody is having a great day. I am working with a client on a new position that I wanted to tell you all about. My client is a private pathology lab located in Coastal Texas. This is a dayshift (early morning) full time permanent position. It is M-F with occasional Saturdays. My client offers a competitive pay, benefits and relocation assistance. Requirements: ASCP or elig. 1-2 yrs plus Routine Histology experience (including hand staining) IHC is a plus. This is an immediate opening but my client is willing to consider candidates who prefer to start work after the holidays as well. If you or anyone you know might be interested please contact me - Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. *Thanks for your time and Have a great day!* Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Timothy.Morken <@t> ucsfmedctr.org Mon Dec 6 14:34:57 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Mon Dec 6 14:35:14 2010 Subject: [Histonet] Competencies In-Reply-To: References: Message-ID: We keep ours for two years. We are under JC and that is how far back they go when checking. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Wilder Sent: Thursday, December 02, 2010 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Competencies When a Histotech leaves their place of employment - does anyone know if their competencies need to be kept for any period of time or can the competencies be disgarded after their departure? Thanks for your help! Paula Wilder St. Joseph Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Mon Dec 6 14:35:10 2010 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Dec 6 14:35:15 2010 Subject: [Histonet] Problems with histonet In-Reply-To: <884837.79871.qm@web113812.mail.gq1.yahoo.com> References: <884837.79871.qm@web113812.mail.gq1.yahoo.com> Message-ID: I haven't received anything since Wednesday, but my email is blowing up now with Histonet stuff! I thought it was just my computer =) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Friday, December 03, 2010 6:54 PM To: histonet Subject: [Histonet] Problems with histonet I just made 2 posts today and they are not showing up. Is any one having problems too? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Herrick.James <@t> mayo.edu Mon Dec 6 14:39:01 2010 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Mon Dec 6 14:39:06 2010 Subject: [Histonet] Problems with histonet In-Reply-To: <884837.79871.qm@web113812.mail.gq1.yahoo.com> References: <884837.79871.qm@web113812.mail.gq1.yahoo.com> Message-ID: <7267A64D75F58241B577876D8A885631038FE3A6@msgebe41> I made a post this morning as well, and it didn't show. However, I did receive a response. Jim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Friday, December 03, 2010 6:54 PM To: histonet Subject: [Histonet] Problems with histonet I just made 2 posts today and they are not showing up. Is any one having problems too? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Mon Dec 6 15:27:29 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Dec 6 15:27:36 2010 Subject: [Histonet] Veterinary SOPs Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E474DF@nmdamailsvr.nmda.ad.nmsu.edu> For those of you who work in a veterinary diagnostic lab where there is a BSL-2 and/or BSL-3 necropsy room, I could use some assistance with designing some SOPs for "maintenance" of those rooms. For example, what are your daily routines and/or procedures to ensure that those rooms are clean, temperatures checked, setup requirements met, and so forth. This is not writing pathologists' SOPs for specific necropsy procedures, but more the day-to-day operation and maintenance of the rooms in which a pathologist would work. I would appreciate any input and will share with those who ask. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From akemiat3377 <@t> yahoo.com Mon Dec 6 16:04:44 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Mon Dec 6 16:04:49 2010 Subject: [Histonet] Eosin in Tissue Processor Message-ID: <768155.14922.qm@web113817.mail.gq1.yahoo.com> Happy Monday Everyone! I guess I am having a old persons momment!? I forgot how much eosin and what % to put in the last 100% of the Tissue Processor to show-up small transparent bx's.? Can one of you wonderful people tell me please.? The processor I am using is a VIP 5. ? Thanks a Bunch! ?Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com From sfeher <@t> CMC-NH.ORG Mon Dec 6 16:15:40 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Dec 6 16:15:44 2010 Subject: [Histonet] Hemoglobin A In-Reply-To: References: Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC243869C4@exchange.cmc-nh.org> We just got some from BioGenex order number is AR021-5R. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, December 02, 2010 5:52 PM To: Histonet Subject: [Histonet] Hemoglobin A Howdy histoland, does anyone know where to get Hemoglobin A? Dako doesn't have it any more and we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc, etc, etc, Biogenex. No luck. Thanks Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Mon Dec 6 16:20:33 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Dec 6 16:20:36 2010 Subject: [Histonet] Workload Recording In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD22@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFD22@LBEXCH01.hchd.local> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC243869C5@exchange.cmc-nh.org> If you are set up for your techs to bar code scan their blocks before they cut, you can keep track of it that way. We have this set up in our LIS. Our techs scan at every station (embedding, cutting, staining, etc) so I can track specimens in addition to keeping statistics. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, December 03, 2010 5:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Workload Recording Hello to all in histoland. Does anyone keep a log of how many blocks that their techs cut. My boss asked me do I keep this type of information. Anyone doing workload recording? Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim.tournear <@t> yahoo.com Mon Dec 6 17:12:15 2010 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Mon Dec 6 17:12:20 2010 Subject: [Histonet] New Histology Field Technical Role Based in AZ/TX/Southern CA In-Reply-To: <2cf5cbc66f74ecede72ddd7217c9ac46@mail.gmail.com> Message-ID: <903203.15151.qm@web120214.mail.ne1.yahoo.com> This is currently my territory. I'm relocating to Florida,?so if anyone wants more details about the job, contact me at this address and we'll touch base....this job is fun.... ~Kim~? ? OU ROCKS!!!! ~Don't be afraid your life will end, be afraid it will never begin~ --- On Thu, 12/2/10, Matt Ward wrote: From: Matt Ward Subject: [Histonet] New Histology Field Technical Role Based in AZ/TX/Southern CA To: histonet@lists.utsouthwestern.edu Date: Thursday, December 2, 2010, 9:33 PM Good Afternoon Everyone! We have had a world leader open a new Field Technical Histology position that will cover the Pacific Region. The best place to live for this position would be Phoenix, Los Angeles, Or Dallas, and it will focus on Core Histology. This is perfect for a Histotech that is looking for an opportunity to get out of the lab in into the field with a growing company that offers promotion. Please contact me directly by sending me a copy of your resume to mw@personifysearch.com. Thanks! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Mon Dec 6 17:23:42 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Dec 6 17:24:08 2010 Subject: [Histonet] Hemoglobin A In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC243869C4@exchange.cmc-nh.org> References: <0908FC0A43B87A4FB60EDCCA06AABC243869C4@exchange.cmc-nh.org> Message-ID: <3703005A6B5548308A32F553BF046A7B@JoePC> thanks y'all for the info Joe ----- Original Message ----- From: "Feher, Stephen" To: "Joe Nocito" ; "Histonet" Sent: Monday, December 06, 2010 4:15 PM Subject: RE: [Histonet] Hemoglobin A We just got some from BioGenex order number is AR021-5R. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, December 02, 2010 5:52 PM To: Histonet Subject: [Histonet] Hemoglobin A Howdy histoland, does anyone know where to get Hemoglobin A? Dako doesn't have it any more and we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc, etc, etc, Biogenex. No luck. Thanks Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From axp969 <@t> psu.edu Mon Dec 6 17:45:04 2010 From: axp969 <@t> psu.edu (ANJUM PARKAR) Date: Mon Dec 6 17:45:11 2010 Subject: [Histonet] Problems with histonet In-Reply-To: 201012062035.oB6KZ8n54214884@tr10n05.aset.psu.edu References: <201012062035.oB6KZ8n54214884@tr10n05.aset.psu.edu> Message-ID: <1291679104l.1253468l.0l@psu.edu> I have the exact same problem. I had send out fur posts none showed up. Anjum On Mon, Dec 6, 2010 03:35 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. FW: TBS Histology Equipment (Damaris Beil) > 2. Changing solutions on VIP processors. (Angela Bitting) > 3. Eosin in Tissue Processor (Akemi Allison) > 4. Workload Recording (Scott, Allison D) > 5. Eosin in Tissue Processor (Akemi Allison) > 6. Problems with histonet (Akemi Allison) > 7. Test - please disregard (histotech@imagesbyhopper.com) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Fri, 3 Dec 2010 13:30:49 -0500 >From: Damaris Beil >Subject: [Histonet] FW: TBS Histology Equipment >To: histonet >Message-ID: >Content-Type: text/plain; charset="iso-8859-1" > > > > > > > > > >Damaris E. Beil, HT,ASCPcm, HTL >Associates in Dermatology >Histology Laboratory >(407) 846-7546 ext. 3307 > > > > > > >From: dbeil1@hotmail.com >To: histonet@lists.utsouthwestern.edu >Subject: TBS Histology Equipment >Date: Fri, 3 Dec 2010 10:48:04 -0500 > > > > >Hello everyone, > >I was wondering if any of you would be able to give me feedback on the TBS ATP >processor, TEC embedding center, SHUR/Stain H&E stainer, microtomes and >other piece of instrument from TBS/SHUR. > >Thank You, > > > > > >Damaris E. Beil, HT, ASCPcm,HTL > >Associates in Dermatology >Histology Laboratory >(407) 846-7546 ext. 3307 > > > > > > > > >------------------------------ > >Message: 2 >Date: Fri, 03 Dec 2010 14:02:59 -0500 >From: "Angela Bitting" >Subject: [Histonet] Changing solutions on VIP processors. >To: >Message-ID: <4CF8F893.2B7F.00C9.1@geisinger.edu> >Content-Type: text/plain; charset="us-ascii" > >Hello All, >I'm involved in a debate with a colleague about how frequently the tissue >processors need to be changed. >We do roughly 1000 blocks a day on various runs, utilizing 7 tissue processors. >About 100 of those are microwave processed, so I'm subtracting those from the >equation. > >So 900 blocks (mixed tissues, no breast) divided by 6 conventional >processors is 150 blocks each. > >I think it's poor reagent management to change a VIP after 150 blocks. Does >anyone disagree with me? >If these were processing very fatty tissue, I might think differently, but >that's not the case. > >All opinions are welcome. >Thanks, >Angie > >Angela Bitting, HT(ASCP), QIHC >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > >No trees were hurt in the sending of this email >However many electrons were severly inconvienienced! > > >IMPORTANT WARNING: The information in this message (and the documents >attached to it, if any) is confidential and may be legally privileged. It >is intended solely for the addressee. Access to this message by anyone else is >unauthorized. If you are not the intended recipient, any disclosure, copying, >distribution or any action taken, or omitted to be taken, in reliance on it is >prohibited and may be unlawful. If you have received this message in error, >please delete all electronic copies of this message (and the documents >attached to it, if any), destroy any hard copies you may have created and >notify me immediately by replying to this email. Thank you. >-------------- next part -------------- >BEGIN:VCARD >VERSION:2.1 >X-GWTYPE:USER >FN:Bitting, Angela >TEL;WORK:570-271-6844 >ORG:;Histology >EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu >N:Bitting;Angela >END:VCARD > > >------------------------------ > >Message: 3 >Date: Fri, 3 Dec 2010 12:26:39 -0800 (PST) >From: Akemi Allison >Subject: [Histonet] Eosin in Tissue Processor >To: histonet >Message-ID: <374906.66310.qm@web113814.mail.gq1.yahoo.com> >Content-Type: text/plain; charset=iso-8859-1 > >Happy Friday Everyone, > >I guess I am having a old persons momment!? I forgot how much eosin and what % >to put in the last 100% of the Tissue Processor to show-up small transparent >bx's.? Can one of you wonderful people tell me please.? I tissue processor is a >VIP 5. > >Thanks a bunch! >Akemi Allison BS, HT(ASCP)HTL >Director >Phoenix Lab Consulting >E-Mail: akemiat3377@yahoo.com > > > > >------------------------------ > >Message: 4 >Date: Fri, 3 Dec 2010 16:09:36 -0600 >From: "Scott, Allison D" >Subject: [Histonet] Workload Recording >To: >Message-ID: > <1872B4A455B7974391609AD8034C79FC026DFD22@LBEXCH01.hchd.local> >Content-Type: text/plain; charset="us-ascii" > >Hello to all in histoland. Does anyone keep a log of how many blocks >that their techs cut. My boss asked me do I keep this type of >information. Anyone doing workload recording? > >Allison Scott HT(ASCP) >Histology Supervisor >LBJ Hospital >CONFIDENTIALITY NOTICE: >If you have received this e-mail in error, please immediately notify the >sender by return e-mail and delete this e-mail and any attachments from >your computer system. > >To the extent the information in this e-mail and any attachments contain >protected health information as defined by the Health Insurance Portability >and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 >CFR Parts 160 and >164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or >privileged. This e-mail may also be confidential and/or privileged under >Texas law. The e-mail is for the use of only the individual or entity named >above. If you are not the intended recipient, or any authorized >representative of the intended recipient, you are hereby notified that any >review, dissemination or copying of this e-mail and its attachments is >strictly prohibited. > > > >------------------------------ > >Message: 5 >Date: Fri, 3 Dec 2010 16:39:12 -0800 >From: Akemi Allison >Subject: [Histonet] Eosin in Tissue Processor >To: Histonet >Message-ID: <69EEA8DB-7A28-410D-AA23-C4E4A8877243@yahoo.com> >Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > >Happy Friday Everyone, > >I am sending this inquiry again. For some reason this post did not >show I my histonet emaiI. I guess I am having a old persons >momment! I forgot how much eosin and what % to put in the last 100% >of the Tissue Processor to show-up small transparent bx's. Can one >of you wonderful people tell me please. The tissue processor is a >VIP 5. > >Thanks a bunch! > > >Akemi Allison BS, HT (ASCP) HTL >Director >Phoenix Lab Consulting >Tele: 408.335.9994 >E-Mail: akemiat3377@yahoo.com > > > >------------------------------ > >Message: 6 >Date: Fri, 3 Dec 2010 16:53:31 -0800 (PST) >From: Akemi Allison >Subject: [Histonet] Problems with histonet >To: histonet >Message-ID: <884837.79871.qm@web113812.mail.gq1.yahoo.com> >Content-Type: text/plain; charset=us-ascii > >I just made 2 posts today and they are not showing up. Is any one having >problems too? > Akemi Allison BS, HT(ASCP)HTL >Director >Phoenix Lab Consulting >E-Mail: akemiat3377@yahoo.com > > > > >------------------------------ > >Message: 7 >Date: Sat, 04 Dec 2010 08:39:55 -0500 >From: >Subject: [Histonet] Test - please disregard >To: >Message-ID: <6A77F54DB40549CB8EED8A2E81C13DCE@hopperPC> >Content-Type: text/plain; charset=us-ascii > >This is a test to see if my email is working properly. Please disregard. > >Thanks! > > > > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 85, Issue 4 >*************************************** > > > -- From muskakim <@t> yahoo.com Mon Dec 6 18:53:33 2010 From: muskakim <@t> yahoo.com (kimberly muska) Date: Mon Dec 6 18:53:38 2010 Subject: [Histonet] clearium Message-ID: <101151.99654.qm@web52004.mail.re2.yahoo.com> ??????????? Does anyone have any experience with the Surgipath product ?Clearium?? It is a mounting medium that clears and coverslips in ?one easy step? eliminating the need for xylene or xylene substitutes following staining. Does it decrease the shelf life of the slides? Is it very expensive? In my lab we use a microwave processor so if I used this product I would have no need for Xylene or substitutes at all. Thanks, Kim Ruth HT(ASCP) From amosbrooks <@t> gmail.com Mon Dec 6 15:36:21 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Dec 6 20:36:37 2010 Subject: [Histonet] Hellerstrom & Hellman anyone? Message-ID: <201012061636.22299.amosbrooks@gmail.com> Thanks, I did, with 50% nitric acid. I would have thought that if there was a problem with the glassware it would have shown up in the silver nitrate overnight. The silver solution was clear after incubating. The milky mess didn't happen until after washing in 95 ETOH then placing in the developing solution. Just to be sure though I'll make sure to clean the glassware longer than last time. I'll also run a couple of controls and take one out after an hour to reduce just it really is over impregnated. Thanks again, Amos Message: 16 Date: Wed, 1 Dec 2010 20:58:20 -0600 From: "Ingles Claire " Subject: RE: [Histonet] Hellerstrom & Hellman anyone? To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Did you acid clean your glassware before hand? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Amos Brooks Sent: Wed 12/1/2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hellerstrom & Hellman anyone? Hi, I just did a Hellerstrom & Hellman alcoholic silver stain for pancreatic D- cells. I followed the method described by Bancroft & Stevens. When the slides were placed into the reducing solution they instantly precipitated into a toasty mess, the solution turned milky brown and the slides (even around the sections) picked up a lot of silver precipitate. If anyone has done this of could offer suggestions about it, I'd really like to trouble shoot the stain a bit. A bit of background about the stain that I thought I should add. According to the published method I used a 10% silver solution in ETOH with 100ul of 1M nitric acid. The pH of the solution was 5.0. The reducing solution was alcoholic formalin with 5% pyrogallic acid. I thought the silver solution seemed really concentrated (It hardly even disolved). Incubating such a solution overnight at 37 deg C (as indicated in the method) seemed a bit much. Has anyone heard of modifications of this stain? There is no sense reinventing the wheel, but I certainly don't want to repeat it knowing that it will innevitably do the same thing again unless there is something glaringly obvious that I did wrong. Thanks, Amos From wdesalvo.cac <@t> hotmail.com Mon Dec 6 20:42:51 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Mon Dec 6 20:42:56 2010 Subject: [Histonet] Workload Recording In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD22@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFD22@LBEXCH01.hchd.local> Message-ID: There are now several commercial workload/work flow management systems available, if you are bar coded for your blocks and slides (Ventana, Thermo, Leica and Dako). These "automated " processes can simplify the capture of metrics for individuals, but not many have robust report functions. You could also use a manual system for capturing metrics, but this approach will require some additional effort over and above what is required for the automated systems. Both approaches will provide you a detailed look at what can be done, what is being done and what should be done at each step of your process and by whom. Productivity is a key management tool and with all the economy and industry pressures being applied, now being looked at closely for the Histology lab. I have been manually capturing workload metrics for accessioning, grossing, embedding and microtomy for 7+ years. It can be very useful when working with management to develop the correct staffing needs for the manual tasks in Histology. Workload recording allows you to better manage your process and employees. The data collected can be sliced and diced in many ways and I strongly suggest you collect on a daily basis. Each employee should know exactly what is expected of them for productivity and quality and you can only provide that direction when you have useful data. Once you have the data and create your reports, make sure you communicate with all employees regularly and continually. All that said, what are you trying to accomplish with creating workload metrics and/or what does your boss want to accomplish? It is important to understand that just capturing the workload metrics mean nothing without coupling quality standards. I believe that since we are very manual in our unit (case/specimen/block/slide) production, you must not just look at the production factor, you must apply a quality factor/standard, before setting any production standards. Quality must come first. last and always in your process development. Of course, that will mean you will need to take the next step and develop a process for capturing, trending and correcting all Mislabel, Procedural and Technical errors produced at each step of the process and ensure that the production metric and quality metric, when combined will lead and direct you to improved quality and measurable standards. I find that having the workload and quality metrics available provides valuable information for development of staff, training/competency documentation, SOP's and justifying equipment, consumables and reagent purchases. I learned from my CEO that "You cannot manage what you do not measure". I would be more than happy to discuss this process with you off line, just send me an e-mail to start the process. William DeSalvo, B.S., HTL(ASCP) wdesalvo.cac@hotmail.com > Date: Fri, 3 Dec 2010 16:09:36 -0600 > From: Allison_Scott@hchd.tmc.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Workload Recording > > Hello to all in histoland. Does anyone keep a log of how many blocks > that their techs cut. My boss asked me do I keep this type of > information. Anyone doing workload recording? > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gervaip <@t> aol.com Mon Dec 6 21:00:46 2010 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Mon Dec 6 21:01:10 2010 Subject: [Histonet] Greta HT Position Message-ID: <5e995.28574d7b.3a2efd5e@aol.com> sounds like a full time+ job In a message dated 12/6/2010 2:29:55 P.M. Central Standard Time, mhale@carisls.com writes: Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Mon Dec 6 21:05:21 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Mon Dec 6 21:05:26 2010 Subject: [Histonet] Changing solutions on VIP processors. In-Reply-To: <4CF8F893.2B7F.00C9.1@geisinger.edu> References: <4CF8F893.2B7F.00C9.1@geisinger.edu> Message-ID: I suggest to develop the best quality standard for processing, you must first develop or decide what the quality parameters will be that dictates a rotation or changing of a reagent. Have you determined what constitutes a "dirty" or unacceptable reagent? Create and run a test method validation protocol to define the quality parameters. Your particular mix of tissues types and the number of cassettes processed are important indicators, but what how do you determine when a reagent is unacceptable? Have you measured the particulate matter, concentration of solution, carryover from one reagent type the the next? Define the parameters first and then you can set the standard, for your lab and mix of tissues, that will provide consistent and quality results, each and every time. Make sure you include the pathologist, as he/she is a critical end point QC for your process. Make sure you are meeting your most important customer's needs. I believe that testing and then defining set standards will allow you to best manage the reagents used on the processors and the process, while providing the best and consistent quality. Making a guess for when the reagents should be changed for multiple processors or relying on anecdotal information from labs that do not gross the same as our lab, have the same daily tissue sample mix as you lab or have the same quality standards you have, can only lead to inconsistent and possibly wasteful use of reagents or unacceptable processed tissue samples. Use standardization and quality processes to lead you to better quality results. That is always the goal. William DeSalvo, B.S., HTL(ASCP) wdesalvo.cac@hotmail.com Date: Fri, 3 Dec 2010 14:02:59 -0500 From: akbitting@geisinger.edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Changing solutions on VIP processors. Hello All, I'm involved in a debate with a colleague about how frequently the tissue processors need to be changed. We do roughly 1000 blocks a day on various runs, utilizing 7 tissue processors. About 100 of those are microwave processed, so I'm subtracting those from the equation. So 900 blocks (mixed tissues, no breast) divided by 6 conventional processors is 150 blocks each. I think it's poor reagent management to change a VIP after 150 blocks. Does anyone disagree with me? If these were processing very fatty tissue, I might think differently, but that's not the case. All opinions are welcome. Thanks, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Mon Dec 6 21:14:31 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Mon Dec 6 21:14:36 2010 Subject: [Histonet] Competencies In-Reply-To: References: Message-ID: You should consider your own HR and all regulatory agencies requirements before discarding any employee file information. When we were last inspected by CAP, they wanted to see the files for all employees listed on our last submission, plus new additions and they wanted 2 years of data. Normally I would say at least two years fro any paperwork, but it is always best to ask your employee document specialists, HR. There are many different requirements for employee file documentation. If there was a corrective action issue, then we are required to keep files longer. If the employee was involved with formalin, xylene or other concentration testing, then the employee records for the testing results must be kept for 20 years. There are many different needs to consider before discarding employee files and HR and sometimes Risk Management will need to be involved and make the decision on discard. William DeSalvo, B.S., HTL(ASCP) > From: histo20@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 2 Dec 2010 18:18:32 +0000 > Subject: [Histonet] Competencies > > > When a Histotech leaves their place of employment - does anyone know if their competencies need to be kept for any period of time or can the competencies be disgarded after their departure? Thanks for your help! > > Paula Wilder > St. Joseph Medical Center _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> shorememorial.org Tue Dec 7 05:54:26 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Tue Dec 7 05:57:38 2010 Subject: [Histonet] Workload Recording In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD22@LBEXCH01.hchd.local> Message-ID: Allison, I do keep a record of our workload but not for each individual tech in the department. I do not think that this is a requirement. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Scott, Allison D" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Workload Recording 12/03/2010 05:09 PM Hello to all in histoland. Does anyone keep a log of how many blocks that their techs cut. My boss asked me do I keep this type of information. Anyone doing workload recording? Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From muskakim <@t> yahoo.com Tue Dec 7 06:36:09 2010 From: muskakim <@t> yahoo.com (kimberly muska) Date: Tue Dec 7 06:36:13 2010 Subject: [Histonet] clearium In-Reply-To: <101151.99654.qm@web52004.mail.re2.yahoo.com> Message-ID: <698173.83592.qm@web52001.mail.re2.yahoo.com> excuse me, I will still need xylen for the beginning of my staining process but my questions about cost and quality still apply --- On Mon, 12/6/10, kimberly muska wrote: From: kimberly muska Subject: [Histonet] clearium To: histonet@lists.utsouthwestern.edu Date: Monday, December 6, 2010, 7:53 PM ??????????? Does anyone have any experience with the Surgipath product ?Clearium?? It is a mounting medium that clears and coverslips in ?one easy step? eliminating the need for xylene or xylene substitutes following staining. Does it decrease the shelf life of the slides? Is it very expensive? In my lab we use a microwave processor so if I used this product I would have no need for Xylene or substitutes at all. Thanks, Kim Ruth HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Arlene.Armandi <@t> cshs.org Tue Dec 7 07:15:03 2010 From: Arlene.Armandi <@t> cshs.org (Armandi, Arlene) Date: Tue Dec 7 07:15:10 2010 Subject: [Histonet] Workload Recording In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD22@LBEXCH01.hchd.local> Message-ID: We have a "Workload Tally Sheet" that each employee completes daily, and turns in at the end of the day. If you are interested, I would be happy to send it to you. Arlene Armandi, HTL(ASCP) Histology Supervisor Cedars-Sinai Medical Center E-mail: arlene.armandi@cshs.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, December 03, 2010 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Workload Recording Hello to all in histoland. Does anyone keep a log of how many blocks that their techs cut. My boss asked me do I keep this type of information. Anyone doing workload recording? Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. From Randi.Hayes <@t> horizonnb.ca Tue Dec 7 07:25:17 2010 From: Randi.Hayes <@t> horizonnb.ca (Hayes, Randi (HorizonNB)) Date: Tue Dec 7 07:25:27 2010 Subject: [Histonet] Re: positive slides In-Reply-To: Message-ID: The VWR slides (superfrost catalogue #48311-703) are the only slide that is validated to be used on the Ventana platforms. The problem with these slides is that they are hydrophobic. They do hold onto the tissue really well, but when you dip them in a water bath or pipette fluid on them while they are sitting on the stainer, you will notice that the fluid pools and does not spread evenly over the entire slide. This is causing problems with consistency in staining. Another site in my area has had problems with their FISH staining as a result of these slides as well. I would like to be able to tell you that Ventana has been able to provide an alternative for this problem, but I can't. *sigh* Now, if you're ambitious and have loads of time on your hands (sarcasm intended!), Surgipath does produce a charged slide that is hydrophilic. They are called Apex Superior Adhesive Slides. My rep sent me a couple of boxes to try, but with our volume of IHC staining and only one instrument, we just haven't been able to squeeze in a validation study. If you try to do this, please let me know how you make out! Good luck! Randi Hayes Histology Supervisor Horizon Health Network - Moncton Zone Moncton, NB Canada randi.hayes@HorizoNB.ca ------- Horizon Health Network Disclaimer ------- This e-mail communication (including any or all attachments) is intended only for the use of the person or entity to which it is addressed and may contain confidential and/or privileged material. If you are not the intended recipient of this e-mail, any use, review, retransmission, distribution, dissemination, copying, printing, or other use of, or taking of any action in reliance upon this e-mail, is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete the original and any copy of this e-mail and any printout thereof, immediately. Your co-operation is appreciated. Le pr?sent courriel (y compris toute pi?ce jointe) s'adresse uniquement ? son destinataire, qu'il soit une personne ou un organisme, et pourrait comporter des renseignements privil?gi?s ou confidentiels. Si vous n'?tes pas le destinataire du courriel, il est interdit d'utiliser, de revoir, de retransmettre, de distribuer, de diss?miner, de copier ou d'imprimer ce courriel, d'agir en vous y fiant ou de vous en servir de toute autre fa?on. Si vous avez re?u le pr?sent courriel par erreur, pri?re de communiquer avec l'exp?diteur et d'?liminer l'original du courriel, ainsi que toute copie ?lectronique ou imprim?e de celui-ci, imm?diatement. Nous sommes reconnaissants de votre collaboration. From Ronald.Houston <@t> nationwidechildrens.org Tue Dec 7 07:32:08 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Dec 7 07:32:15 2010 Subject: [Histonet] Hemoglobin A In-Reply-To: References: Message-ID: Cell Marque does have a rabbit monoclonal antibody to Hemoglobin A, cat# 360R Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, December 02, 2010 5:52 PM To: Histonet Subject: [Histonet] Hemoglobin A Howdy histoland, does anyone know where to get Hemoglobin A? Dako doesn't have it any more and we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc, etc, etc, Biogenex. No luck. Thanks Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From mike <@t> pathview.com Tue Dec 7 07:45:46 2010 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Dec 7 07:46:28 2010 Subject: [Histonet] Workload Recording In-Reply-To: References: <1872B4A455B7974391609AD8034C79FC026DFD22@LBEXCH01.hchd.local> Message-ID: <003e01cb9615$16058050$421080f0$@pathview.com> Very well stated! If you're going to count 'units processed', be it specimen, blocks, or slides, those numbers lose their value if you're not somehow measuring quality issues as well. In addition to the 'middleware' solutions provided by the vendors mentioned, you need to make sure that your next LIS includes this functionality as well. Quality and metrics are important to the entire department and issues which occur in one area are often affected by actions that have occurred prior to or after the histology area. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, December 06, 2010 8:43 PM To: allison_scott@hchd.tmc.edu; histonet Subject: RE: [Histonet] Workload Recording There are now several commercial workload/work flow management systems available, if you are bar coded for your blocks and slides (Ventana, Thermo, Leica and Dako). These "automated " processes can simplify the capture of metrics for individuals, but not many have robust report functions. You could also use a manual system for capturing metrics, but this approach will require some additional effort over and above what is required for the automated systems. Both approaches will provide you a detailed look at what can be done, what is being done and what should be done at each step of your process and by whom. Productivity is a key management tool and with all the economy and industry pressures being applied, now being looked at closely for the Histology lab. I have been manually capturing workload metrics for accessioning, grossing, embedding and microtomy for 7+ years. It can be very useful when working with management to develop the correct staffing needs for the manual tasks in Histology. Workload recording allows you to better manage your process and employees. The data collected can be sliced and diced in many ways and I strongly suggest you collect on a daily basis. Each employee should know exactly what is expected of them for productivity and quality and you can only provide that direction when you have useful data. Once you have the data and create your reports, make sure you communicate with all employees regularly and continually. All that said, what are you trying to accomplish with creating workload metrics and/or what does your boss want to accomplish? It is important to understand that just capturing the workload metrics mean nothing without coupling quality standards. I believe that since we are very manual in our unit (case/specimen/block/slide) production, you must not just look at the production factor, you must apply a quality factor/standard, before setting any production standards. Quality must come first. last and always in your process development. Of course, that will mean you will need to take the next step and develop a process for capturing, trending and correcting all Mislabel, Procedural and Technical errors produced at each step of the process and ensure that the production metric and quality metric, when combined will lead and direct you to improved quality and measurable standards. I find that having the workload and quality metrics available provides valuable information for development of staff, training/competency documentation, SOP's and justifying equipment, consumables and reagent purchases. I learned from my CEO that "You cannot manage what you do not measure". I would be more than happy to discuss this process with you off line, just send me an e-mail to start the process. William DeSalvo, B.S., HTL(ASCP) wdesalvo.cac@hotmail.com > Date: Fri, 3 Dec 2010 16:09:36 -0600 > From: Allison_Scott@hchd.tmc.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Workload Recording > > Hello to all in histoland. Does anyone keep a log of how many blocks > that their techs cut. My boss asked me do I keep this type of > information. Anyone doing workload recording? > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify > the sender by return e-mail and delete this e-mail and any attachments > from your computer system. > > To the extent the information in this e-mail and any attachments > contain protected health information as defined by the Health > Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL > 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and > Safety Code, it is confidential and/or privileged. This e-mail may > also be confidential and/or privileged under Texas law. The e-mail is > for the use of only the individual or entity named above. If you are > not the intended recipient, or any authorized representative of the > intended recipient, you are hereby notified that any review, > dissemination or copying of this e-mail and its attachments is strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Tue Dec 7 08:12:36 2010 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Tue Dec 7 08:12:46 2010 Subject: [Histonet] RE: positive slides In-Reply-To: References: Message-ID: We tried the Surgipath slides, unfortunately, they led to even more inconsistent staining than the superfrost plus. We haven't had any issues with the superfrost plus slides since early October. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (HorizonNB) Sent: Tuesday, December 07, 2010 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: positive slides The VWR slides (superfrost catalogue #48311-703) are the only slide that is validated to be used on the Ventana platforms. The problem with these slides is that they are hydrophobic. They do hold onto the tissue really well, but when you dip them in a water bath or pipette fluid on them while they are sitting on the stainer, you will notice that the fluid pools and does not spread evenly over the entire slide. This is causing problems with consistency in staining. Another site in my area has had problems with their FISH staining as a result of these slides as well. I would like to be able to tell you that Ventana has been able to provide an alternative for this problem, but I can't. *sigh* Now, if you're ambitious and have loads of time on your hands (sarcasm intended!), Surgipath does produce a charged slide that is hydrophilic. They are called Apex Superior Adhesive Slides. My rep sent me a couple of boxes to try, but with our volume of IHC staining and only one instrument, we just haven't been able to squeeze in a validation study. If you try to do this, please let me know how you make out! Good luck! Randi Hayes Histology Supervisor Horizon Health Network - Moncton Zone Moncton, NB Canada randi.hayes@HorizoNB.ca ------- Horizon Health Network Disclaimer ------- This e-mail communication (including any or all attachments) is intended only for the use of the person or entity to which it is addressed and may contain confidential and/or privileged material. If you are not the intended recipient of this e-mail, any use, review, retransmission, distribution, dissemination, copying, printing, or other use of, or taking of any action in reliance upon this e-mail, is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete the original and any copy of this e-mail and any printout thereof, immediately. Your co-operation is appreciated. Le pr?sent courriel (y compris toute pi?ce jointe) s'adresse uniquement ? son destinataire, qu'il soit une personne ou un organisme, et pourrait comporter des renseignements privil?gi?s ou confidentiels. Si vous n'?tes pas le destinataire du courriel, il est interdit d'utiliser, de revoir, de retransmettre, de distribuer, de diss?miner, de copier ou d'imprimer ce courriel, d'agir en vous y fiant ou de vous en servir de toute autre fa?on. Si vous avez re?u le pr?sent courriel par erreur, pri?re de communiquer avec l'exp?diteur et d'?liminer l'original du courriel, ainsi que toute copie ?lectronique ou imprim?e de celui-ci, imm?diatement. Nous sommes reconnaissants de votre collaboration. From rmweber113 <@t> comcast.net Tue Dec 7 08:38:44 2010 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Tue Dec 7 08:39:17 2010 Subject: [Histonet] HT Manager in NJ Message-ID: <1324657341.334891.1291732724532.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> We are currently looking for a HT Manager in a newly developed urology practice laboratory in North Jersey.? Candidate must be ASCP certified with at 2 years experience .? This is a full time position Monday to Friday.? Offer a full benefit package.? Please send resume to fax number below or call. Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC ?phone 732 814-1170 fax 267 722-8308 From talulahgosh <@t> gmail.com Tue Dec 7 08:50:22 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Dec 7 08:50:50 2010 Subject: [Histonet] OT? protein translation music Message-ID: http://bit.ly/gk2iaa In the style of the newer science raps, here is a 70's version of protein translation. The explanation goes until 3:40, the music starts afterwards. Who knew protein translation was so groovy? I wish I had an mp3 of this!! Emily "It was more probable that Lord Aylesford was in India on account of his intrinsic merits, i.e. his ability to crawl about fashionable drawing rooms on all fours and his willingness during these excursions to be beaten about the rump by his fun-loving friends." -Ronald Persall, The Worm in the Bud, The World of Victorian Sexuality From 1dpeterson <@t> meriter.com Tue Dec 7 11:20:01 2010 From: 1dpeterson <@t> meriter.com (Peterson, Dan) Date: Tue Dec 7 11:23:13 2010 Subject: [Histonet] Histology position Message-ID: <466B666475DE6547BBB0641E540A4BB5083FB70337@EXVS1.meriter.com> Part-time position in Madison, WI .6 FTE Looking for someone to work early shift 4-5 am for approx 4-5 hrs daily If interested go to www.meriter.com for more information. Or you can contact me personally. Daniel R Peterson HT(ASCP) Histopathology Technical Specialist Meriter Laboratories (608) 417-6557 fax (608) 417-6343 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. From MLunetta <@t> luhcares.org Tue Dec 7 12:54:59 2010 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Tue Dec 7 12:59:13 2010 Subject: [Histonet] Manual Copper stain Message-ID: <4CFE2094020000A800050AF2@ns.luhcares.org> A. EQUIPMENT ? Coplin Jars ? Graduated Cylinders B. REAGENTS RHODANINE SATURATED SOLUTION (stock) p-dimethylaminobenxalrhodanine .3 gm Absolute alcohol 100 ml Let the solution sit to allow sediment to fall to the bottom. RHODANINE SATURATED SOLUTION (working) Rhodanine saturated solution 3 ml Distilled water 40 ml Do not shake stock solution before removing the needed quantity from the top of the solution. Mix the working solution when placing the slides into the coplin jar. DILUTED MAYERS HEMATOXYLIN SOLUTION Mayer*s hematoxylin & distilled water (50:50) 0.5% SODIUM BORATE (borax) AQUEOUS Sodium borate 0.5 gm Distilled water 100 ml C. TISSUE CONTROL Tissue positive for Copper 1. Deparaffinize and hydrate to water 2. Incubate in Rhodanine working solution at 67*C for 1 hour. 3. Wash well in several changes of distilled water. 4. Stain in diluted Mayers hematoxylin for 10 minutes. 5. Rinse with distilled water. 6. Quickly rinse in 0.5% sodium borate. 7. Rinse well with distilled water. 8. Dehydrate, clear and mount with synthetic media D. RESULTS Copper * Bright red to red yellow Nuclei * Light blue REFERENCE Modified for altitude from the Rhodanine Method for Copper: Theory and Practice of Histology Sheehan, Hrapchak, Second Edition, pg 230. Hope this helps, Matt Lunetta BS HT (ASCP) From schlea <@t> sage.edu Tue Dec 7 13:02:51 2010 From: schlea <@t> sage.edu (Anna Schleifstein) Date: Tue Dec 7 13:02:55 2010 Subject: [Histonet] Sale of histology equipment Message-ID: <2695353.66621291748571318.JavaMail.root@ccprodapp22> We are looking to sell several pieces of older histology equipment as follows: AO Microtome Knife Sharpener model 925. (Includes cover and 1 glass hone plate), Tissue Tek Microtome/Cryostat model 4553 (with knife)) and AO Microtome model 820 (includes Specimen Holder, knife and Standard Knife Holder). All items are in great working condition. Please contact me if you are interested. Anna Schleifstein SCA Bio Lab Coordinator Sage Colleges Albany NY 12208 518- 292-1748 schlea@sage.edu From ROrr <@t> northshore.org Tue Dec 7 13:12:15 2010 From: ROrr <@t> northshore.org (Orr, Rebecca) Date: Tue Dec 7 13:13:33 2010 Subject: [Histonet] positive charged slides In-Reply-To: <44b7991a-0624-4180-baa4-b72a71879cab@EXCHCAS01.enhnet.org> Message-ID: I've been vacillating about sharing my observations on this topic, mainly because I don't have a good answer for solving the problems that everyone is describing. I have had similar experiences. I have a validated slide that for me is the lesser of all evils. The slides that work the best for me, BUT STILL GIVE ME FITS are the super frost EXTENDED plus slides. Fisher number 22-034-979. These slides have an extended painted area. This allows for automated stainer labels to fit completely on the paint. I have found with the standard paint, that the labels extend over onto the glass and they just don't stick as well. These semi detached labels might or might not be factoring into the staining issues. So I rule that out. Also, I monitor the lot numbers. I have learned that these lot numbers can give the exact date of manufacture. The first numbers are the month, then day, then year. My boxes of slides also have the month and year of manufacture stamped right on the box.. My main problem with testing slides is that we go through lot numbers so quickly. If I order hordes of the same lot number, then are the slides that I open today as "fresh" as the slides I opened two months ago? I hate to have this attitude, but, then what's next, validating Kleenex we use, too? Do clinical labs have to validate every lot number of test tubes they use? The experts that manufacture these slides claim a specific expiration date. What criteria are they using to determine this date? Does the substance or charge on the slide deteriorate over a period of time, or all of a sudden on the expire date they just stop working? Ugh. I wonder if the scientists who put the chemistry onto these slides actually understand what the real world is going through. I have also observed different problems. Sometimes hydrophobic symptoms with patchy staining, sometimes tissue washing off for no explainable reason (ruled out poor processing). I use two different platforms, one where I immerse slides into a heated HIER bath and then lay flat on a rack for staining, and the other where the slides are placed onto the stainer and all steps are completed on the instrument. I can't pin point one thing these stainers could be doing that is contributing to the problem. The common item is the glass slide. Best I got! Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. From rparker <@t> monell.org Tue Dec 7 13:23:02 2010 From: rparker <@t> monell.org (Rocky Parker) Date: Tue Dec 7 13:29:27 2010 Subject: [Histonet] Reference book for fixatives and their properties Message-ID: Hi everyone, I seem to always need information on different fixatives (half-lives, storage, usage, physical properties, alternatives, etc.), and I wanted to know if any of you use a "bible" of sorts in your lab(s). I have a running tally in my head of problems I've had, things to avoid, etc. over the years. However, I don't have a good, physical resource for use in the lab. For instance, we recently had an issue with fixative mixtures and tissue distortion in addition to some shelf life questions that I'm assuming (?) would be covered in the type of reference I'm trying to find. If you know of a reference right off the top of your head that would have all of this info (and perhaps mention of sectioning techniques for TEM, LM, etc.), I would really appreciate that info. I want a book for my own reference, but I also want to purchase the same resource for our lab. Thanks, Rocky ----------------------------- Postdoctoral Fellow Monell Chemical Senses Center 3500 Market St. Philadelphia, PA 19104 From jengirl1014 <@t> yahoo.com Tue Dec 7 16:36:35 2010 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Tue Dec 7 16:36:38 2010 Subject: [Histonet] (no subject) Message-ID: <998166.65498.qm@web62307.mail.re1.yahoo.com> http://visiopharm-3d.eu/super.php?hotmailID=113 From jengirl1014 <@t> yahoo.com Tue Dec 7 16:45:07 2010 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Tue Dec 7 16:45:12 2010 Subject: [Histonet] (no subject) Message-ID: <87379.87267.qm@web62301.mail.re1.yahoo.com> http://auxilingua.org/super.php?PageID=869 From ccrowder <@t> vetmed.lsu.edu Tue Dec 7 22:05:03 2010 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Tue Dec 7 22:07:13 2010 Subject: [Histonet] Copper Stain Message-ID: Pamela - We stained copper with Uzman's method, manually. It uses rubeanic acid (dithiooxamide) solution. The original procedure takes 2 days, but we were able to modify it to take less time. I can send it to you personally if you want to try it. Copper shows as a fine black precipitate. One of the problems with the stain is the control. Copper leeches out of the tissue when left in formalin too long. So control tissues should be processed and embedded as soon as possible. Cheryl From Ronald.Houston <@t> nationwidechildrens.org Wed Dec 8 07:47:15 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Dec 8 07:47:25 2010 Subject: [Histonet] Copper Stain In-Reply-To: References: Message-ID: To all interested in staining copper, there is an article in the Eur J Histochem 2008; 52: 175-178, "Deparaffination time: a crucial point in histochemical detection of tissue copper" where they use Timm's method. They noticed that the time in xylene was critical to optimal staining. I have contacted them to find out if they noticed the same effect on either rhodanine or rubeanic acid demonstration, but haven't to date had a reply. I can provide the article if anyone is interested. Contact me off-line Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder Sent: Tuesday, December 07, 2010 11:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Copper Stain Pamela - We stained copper with Uzman's method, manually. It uses rubeanic acid (dithiooxamide) solution. The original procedure takes 2 days, but we were able to modify it to take less time. I can send it to you personally if you want to try it. Copper shows as a fine black precipitate. One of the problems with the stain is the control. Copper leeches out of the tissue when left in formalin too long. So control tissues should be processed and embedded as soon as possible. Cheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From MITCHELLJA <@t> email.chop.edu Wed Dec 8 08:51:45 2010 From: MITCHELLJA <@t> email.chop.edu (Mitchell, Janice A) Date: Wed Dec 8 08:52:33 2010 Subject: [Histonet] (no subject) Message-ID: Good Morning Histonetters, Does anyone have any experience with PAXgene tissue kit, a new formalin-less fixative that appears to preserve morphology, RNA, miRNA, and DNA. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Assistant Histology Supervisor Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) From robin_dean <@t> compbio.com Wed Dec 8 10:19:35 2010 From: robin_dean <@t> compbio.com (Robin Dean) Date: Wed Dec 8 10:22:29 2010 Subject: [Histonet] Reg: Beta-2 minroglobin IHC Message-ID: <000c01cb96f3$b4b4d3c0$1e1e7b40$@com> Hi all, We are trying to use beta-2 microglobulin IHC staining to ID human cells in goat joints that were engrafted with human cells some time ago and having nothing but trouble. Tissue was decalcified in immunodecal, formalin-fixed paraffin-embedded tissue. Beta-2 MG antibody is a polyclonal rabbit X human beta-2 antibody from Acris, and we are using DAKO Envision-HRP -con. secondary with DAB as chromogen. IgG isotype controls and DAB alone controls look clean. . Goat joints are falling off the charged slides with heat-induced citrate buffer antigen retrieval so went to enzymatic retrieval to keep them on the slide. o Does enzymatic antigen retrieval damage this epitope?? (previous successful uses of this antibody were with citrate antigen retrieval) o Any suggestions on how to keep sections on slides? Sections are kind of large, but pathologist wants that size. . Didn't get hardly any staining at 2.5 ug/ml of the beta-2MG antibody, but at 5 ug/ml everything stains including all of goat joints. . Are there other or better stains that are used for identifying human cells engrafted into other species (label uniformly most human cells)? I saw mention of some mitochondrial antgen antibodies but no specifics. . Does Beta-2 antibody label only human cells? Seems like there are homologous markers in other species that may label. Currently we aren't using any blocks except peroxidase block. Thought we might try a CAS block to see if that will prevent sticking? To non-human tissue. Any suggestions or help with these problems will be greatly appreciated. We have to get the stain to work and are running out of ideas. Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com From alisha <@t> ka-recruiting.com Wed Dec 8 10:51:31 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Dec 8 10:51:08 2010 Subject: [Histonet] GM of Pathology Operations in North Carolina Message-ID: <523114481.1291827091345.JavaMail.cfservice@SL4APP1> Dear Histonet Subscribers, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few histotechnician, histotechnologist, and cytotechnologist openings across the country. Our clients typically assist with relocation expenses. One particular client I am working with is looking for a General Manager of Anatomic Pathology Operations for an anatomic pathology lab in NC. This laboratory is known for its cutting edge technology, LIS system, and its automation. They are looking for someone with 10-20+ years experience in histology/pathology, preferably an HTL(ASCP) certification, at least 10+ years experience in management/operations, and someone who is a strong leader with excellent communication skills. This is a high level pathology position and offers an excellent compensation and benefits package. If interested, please email me your resume to alisha@ka-recruiting.com. Below is a list of some of the opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current HT and HTL Opportunities: 1. Histology Manager - MI 2. Histology Supervisor - NV 3. Histology Supervisor (must have HTL) - Atlanta, GA 4. Histology Supervisor - Central GA 5. Histotech - NYC, NY 6. Grossing Tech - NYC, NY 7. Histotech - IN 8. Histotech - SC 9. Histotech - MA 10. Pathologist's Assistant - NV 11. Pathologist's Assistant - Maine If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From liz <@t> premierlab.com Wed Dec 8 11:06:27 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Dec 8 11:02:59 2010 Subject: [Histonet] Reg: Beta-2 minroglobin IHC In-Reply-To: <000c01cb96f3$b4b4d3c0$1e1e7b40$@com> Message-ID: Robin Have you tried other retrieval methods, its best to normally try to stay away from HIER on bone but sometimes you have to use it. If you have not already tried other retrieval methods I would try no retrieval, proteinase K and then trypsin or pronase, my favorite is proteinase K. Since the samples have been decaled we have sometimes noticed in our hands that the nuclei can get "chewed up" a bit with enzyme retrieval so if you notice that you may need to back off on the time of retrieval, just as long as you do not loose staining intensity. If this antibody will not work with any other retrieval method then you will have to use HIER retrieval. Instead of retrieving at 95 or 100C or even higher I would go with 70C for several hours (2-3) you are going to have to pilot this. That's what we do here for any sample has a tendency to lift or distort when retrieved (bone, skin). You may then be able to run the slides on a stainer but again if you see tissue detachment you are going to have to run them by hand. We use a good superfrost plus slide such as histobond for bone and if we are running into problems with tissue lifting we let the unstained slides sit for a few days even a week before we stain and we find that that helps also. There is a human mitochondria antibody that we have used in the past in rat samples, but I can't remember what retrieval method works. There is also a human nuclear antibody which works well on frozen sections but can be tricky on paraffin, we have not had much success with it on paraffin sections. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin Dean Sent: Wednesday, December 08, 2010 9:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reg: Beta-2 minroglobin IHC Hi all, We are trying to use beta-2 microglobulin IHC staining to ID human cells in goat joints that were engrafted with human cells some time ago and having nothing but trouble. Tissue was decalcified in immunodecal, formalin-fixed paraffin-embedded tissue. Beta-2 MG antibody is a polyclonal rabbit X human beta-2 antibody from Acris, and we are using DAKO Envision-HRP -con. secondary with DAB as chromogen. IgG isotype controls and DAB alone controls look clean. . Goat joints are falling off the charged slides with heat-induced citrate buffer antigen retrieval so went to enzymatic retrieval to keep them on the slide. o Does enzymatic antigen retrieval damage this epitope?? (previous successful uses of this antibody were with citrate antigen retrieval) o Any suggestions on how to keep sections on slides? Sections are kind of large, but pathologist wants that size. . Didn't get hardly any staining at 2.5 ug/ml of the beta-2MG antibody, but at 5 ug/ml everything stains including all of goat joints. . Are there other or better stains that are used for identifying human cells engrafted into other species (label uniformly most human cells)? I saw mention of some mitochondrial antgen antibodies but no specifics. . Does Beta-2 antibody label only human cells? Seems like there are homologous markers in other species that may label. Currently we aren't using any blocks except peroxidase block. Thought we might try a CAS block to see if that will prevent sticking? To non-human tissue. Any suggestions or help with these problems will be greatly appreciated. We have to get the stain to work and are running out of ideas. Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Dec 8 12:40:36 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Dec 8 12:40:42 2010 Subject: [Histonet] Decalcification Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB529F@PHSXMB30.partners.org> We have small arteries that were FFPE and sectioned that should have been decacified before processing. (The investigator did not indicate as such when submitting them). Cutting was extremely difficult and the sections, as you can imagine, had terrible knife marks and chatter. Of course, they are important (for a paper)! Is there any way tissue, that has been embedded, can be decalcified at this point? Or some other way to treat the block so that we can get better sections? Thanks for all your help. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From liz <@t> premierlab.com Wed Dec 8 12:47:10 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Dec 8 12:43:41 2010 Subject: [Histonet] Decalcification In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB529F@PHSXMB30.partners.org> Message-ID: Try surface decaling the samples in the block. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, December 08, 2010 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Decalcification We have small arteries that were FFPE and sectioned that should have been decacified before processing. (The investigator did not indicate as such when submitting them). Cutting was extremely difficult and the sections, as you can imagine, had terrible knife marks and chatter. Of course, they are important (for a paper)! Is there any way tissue, that has been embedded, can be decalcified at this point? Or some other way to treat the block so that we can get better sections? Thanks for all your help. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Dec 8 12:45:04 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Dec 8 12:45:09 2010 Subject: [Histonet] Decalcification In-Reply-To: References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB529F@PHSXMB30.partners.org> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB52A0@PHSXMB30.partners.org> Could you explain in more detail the process, Liz? Thanks! Peggy -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Wednesday, December 08, 2010 1:47 PM To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Decalcification Try surface decaling the samples in the block. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, December 08, 2010 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Decalcification We have small arteries that were FFPE and sectioned that should have been decacified before processing. (The investigator did not indicate as such when submitting them). Cutting was extremely difficult and the sections, as you can imagine, had terrible knife marks and chatter. Of course, they are important (for a paper)! Is there any way tissue, that has been embedded, can be decalcified at this point? Or some other way to treat the block so that we can get better sections? Thanks for all your help. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From STACEY.LANGENBERG <@t> UCDENVER.EDU Wed Dec 8 13:09:50 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Wed Dec 8 13:10:04 2010 Subject: [Histonet] Decalcification In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB529F@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB529F@PHSXMB30.partners.org> Message-ID: <1556363874.1251161.1291835395252.JavaMail.rim@bda341.bisx.prod.on.blackberry> American master tech makes a great surface decal called Easy cut. Good luck Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: "Sherwood, Margaret " Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Wed, 8 Dec 2010 11:40:36 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Decalcification We have small arteries that were FFPE and sectioned that should have been decacified before processing. (The investigator did not indicate as such when submitting them). Cutting was extremely difficult and the sections, as you can imagine, had terrible knife marks and chatter. Of course, they are important (for a paper)! Is there any way tissue, that has been embedded, can be decalcified at this point? Or some other way to treat the block so that we can get better sections? Thanks for all your help. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Dec 8 15:25:37 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Dec 8 15:25:42 2010 Subject: [Histonet] Surface decal of blocks Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB52A4@PHSXMB30.partners.org> I want to thank the many people who responded to my inquiry re: decalcification of embedded tissues. The responses were most helpful, especially the tips people gave for dealing with the blocks. Our pathology department has RDO and I will use that. I will let you know how the sections come out. Thanks again and Happy Holidays! Peggy P.S. I always find the answers on this Listserver. There are so many experts out there in Histoland! Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From brett_connolly <@t> merck.com Wed Dec 8 16:03:24 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Dec 8 16:03:30 2010 Subject: [Histonet] IHC marker for osteosarcomas Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D7595E6C@usctmx1176.merck.com> Hi all, I need to id remnant human osteosarcoma tumors that were implanted as xenografts in mice and then treated with proprietary cmpds. I am thinking of using vimentin and alkaline phosphatase and would like any other suggestions. Many thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From traczyk7 <@t> aol.com Wed Dec 8 17:34:09 2010 From: traczyk7 <@t> aol.com (traczyk7@aol.com) Date: Wed Dec 8 17:34:14 2010 Subject: [Histonet] Pathology Grossing Manual Message-ID: <8CD65478C096F89-DEC-3B64@webmail-d056.sysops.aol.com> Amazon.com lists "Manual of Surgical Pathology Gross Room Procedures" by Juan Rosai. Of course it's not available. Does anyone know anything about this book? Is it worth tracking down? I need to update & expand an existing lab manual and thought it would be helpful. Any ideas would be greatly appreciated. Dorothy MTA Histology LLC PO Box 602 Point Pleasant, NJ 08742 T:732-899-2912 From szigcs <@t> bio.u-szeged.hu Thu Dec 9 02:04:44 2010 From: szigcs <@t> bio.u-szeged.hu (szigcs@bio.u-szeged.hu) Date: Thu Dec 9 02:04:56 2010 Subject: [Histonet] AChE fiber number measurement Message-ID: <20101209090444.6k1kn0rc0k48g44o@webmail.u-szeged.hu> Dear Histonetters! I would like to ask you to recommend us a reliable method to quantify the AChE fiber depletion in the rat cortex after saorin injection. We have a protocoll to clearly visualize the fine fiber pathways and its working well. We need a method to measure the fiber density, (crossing fiber number etc.) I read some protocolls (to draw a line and count the crossing fiber number). I have photoshop and image J software. I would like to achieve this with the aid of these softwares. Many thanks for any help. Best regards Csaba Szigeti ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From akemiat3377 <@t> yahoo.com Thu Dec 9 07:28:12 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Dec 9 07:28:17 2010 Subject: [Histonet] Robert Lott Message-ID: <39100E99-9422-48BD-9749-9455BF9A9FAE@yahoo.com> Good Morning Histoland! I would like to get a hold of Robert Lott. If any of you know his new work information, please let me know. Hey Bobby, if you see this please give me your new contact information. I have some questions for you. Thanks a bunch! Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From abeharry798 <@t> gmail.com Thu Dec 9 08:05:50 2010 From: abeharry798 <@t> gmail.com (Andrea) Date: Thu Dec 9 08:02:41 2010 Subject: [Histonet] ER staining in Tonsil. Message-ID: <353EC1E6-F70F-4D17-BAE1-8D28A629A2EB@gmail.com> Good Morning, Is it usual to get low ER staining in tonsil? From RAusti <@t> lsuhsc.edu Thu Dec 9 08:48:38 2010 From: RAusti <@t> lsuhsc.edu (Austin, Ronald) Date: Thu Dec 9 08:50:13 2010 Subject: [Histonet] EM protocol Message-ID: <77482FC73688964596597C82861BD1600B17DDE9@SH-EXCHBE1.master.lsuhsc.edu> Does anyone have a protocol to do electron microscopy on fingernails? We want to look at the ultrastructure of melanin. Ronald L. Austin Dept. of Pathology LSUHSC Shreveport, LA. rausti@lsuhsc.edu From alisha <@t> ka-recruiting.com Thu Dec 9 09:17:15 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Dec 9 09:16:59 2010 Subject: [Histonet] Laboratory Manager of DermPath Lab Opening in NY Message-ID: <911632552.1291907835455.JavaMail.cfservice@SL4APP4> Hi Histonet Members, I am currently working on a Laboratory Manager position in NY. the ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in dermatopathology. This job opportunity is with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From hfedor <@t> jhmi.edu Thu Dec 9 09:47:59 2010 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Dec 9 09:48:04 2010 Subject: [Histonet] Pathology Grossing Manual In-Reply-To: <8CD65478C096F89-DEC-3B64@webmail-d056.sysops.aol.com> References: <8CD65478C096F89-DEC-3B64@webmail-d056.sysops.aol.com> Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D31785596C46@JHEMTEXVS3.win.ad.jhu.edu> http://www.amazon.com/Surgical-Pathology-Dissection-Illustrated-ebook/dp/B000PY3QPM here is the one that we use. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of traczyk7@aol.com Sent: Wednesday, December 08, 2010 6:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology Grossing Manual Amazon.com lists "Manual of Surgical Pathology Gross Room Procedures" by Juan Rosai. Of course it's not available. Does anyone know anything about this book? Is it worth tracking down? I need to update & expand an existing lab manual and thought it would be helpful. Any ideas would be greatly appreciated. Dorothy MTA Histology LLC PO Box 602 Point Pleasant, NJ 08742 T:732-899-2912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sadey <@t> hotmail.ca Thu Dec 9 12:27:22 2010 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Thu Dec 9 12:27:29 2010 Subject: [Histonet] Cassette and slide labellers compatible with Medi tech Message-ID: Hello, Is anyone using a compatible slide labeller and cassette labeller with Medi tech? We would like it to be interfaced. Thanks in advance. Sheila From gupathlab <@t> gmail.com Thu Dec 9 15:50:53 2010 From: gupathlab <@t> gmail.com (lab operations) Date: Thu Dec 9 15:50:57 2010 Subject: [Histonet] Histology and Cytology Employment Message-ID: Looking for experienced histology technicians and cytotechnologists in Columbus, Ohio for new GU pathology laboratory. Excellent compensation with great benefits. Experience with IHC, FISH, and GU pathology a plus. Send resume or CV to careers@aksm.com. From W.E.J.Hoekert <@t> olvg.nl Fri Dec 10 03:23:19 2010 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Fri Dec 10 03:24:04 2010 Subject: [Histonet] ER staining in Tonsil. References: <353EC1E6-F70F-4D17-BAE1-8D28A629A2EB@gmail.com> Message-ID: <1190CB05C44B13409483514729C2FC360C0B45@PAIT42.olvg.nl> Yes, we see the same. We also see staining in follicles in appendix and colon. ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Andrea Verzonden: do 9-12-2010 15:05 Aan: Histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] ER staining in Tonsil. Good Morning, Is it usual to get low ER staining in tonsil? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From JSilverman <@t> NSHS.edu Fri Dec 10 07:35:39 2010 From: JSilverman <@t> NSHS.edu (Silverman, Jeffrey) Date: Fri Dec 10 07:35:50 2010 Subject: [Histonet] Artisan clips Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE41442F7@SYKECHXVS01.nslijhs.net> Why do you hate the clips? There is a knack to putting them on and removing them without breaking the slide, but it is easy to learn and easy to do. Is that your problem, breakage? Just hook the thing in the back, squeeze the tab in front and gently push down until it snaps in place. To remove, squeeze the tab in front and lift up. What makes me very unhappy though is Dako's impending cessation of IHC support for the Artisan platform. It's great being able to do IHC and routine specials together on the same run. IMHO they should tout that and the superb quality of the IHC and special stains. Jeff Silverman - Happy Artisan user for 11 years From dermpathsy <@t> gmail.com Fri Dec 10 08:30:44 2010 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Fri Dec 10 08:31:07 2010 Subject: [Histonet] Pathology Grossing Manual In-Reply-To: <8CD65478C096F89-DEC-3B64@webmail-d056.sysops.aol.com> References: <8CD65478C096F89-DEC-3B64@webmail-d056.sysops.aol.com> Message-ID: http://amzn.to/gzqCIs Sate On Wed, Dec 8, 2010 at 5:34 PM, wrote: > > Amazon.com lists "Manual of Surgical Pathology Gross Room Procedures" by > Juan Rosai. Of course it's not available. Does anyone know anything about > this book? Is it worth tracking down? I need to update & expand an > existing lab manual and thought it would be helpful. > Any ideas would be greatly appreciated. > Dorothy > -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From rjbuesa <@t> yahoo.com Fri Dec 10 10:00:16 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 10 10:00:19 2010 Subject: [Histonet] Pathology Grossing Manual In-Reply-To: Message-ID: <729784.34519.qm@web65701.mail.ac4.yahoo.com> Yes, Dr. Rosai's book is worth having. Ren? J. --- On Fri, 12/10/10, Sate Hamza wrote: From: Sate Hamza Subject: Re: [Histonet] Pathology Grossing Manual To: traczyk7@aol.com, histonet@lists.utsouthwestern.edu Date: Friday, December 10, 2010, 9:30 AM http://amzn.to/gzqCIs Sate On Wed, Dec 8, 2010 at 5:34 PM, wrote: > > Amazon.com lists "Manual of Surgical Pathology Gross Room Procedures" by > Juan Rosai.? Of course it's not available.? Does anyone know anything about > this book?? Is it worth tracking down?? I need to update & expand an > existing lab manual and thought it would be helpful. > Any ideas would be greatly appreciated. > Dorothy > -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Fri Dec 10 13:41:27 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Fri Dec 10 13:41:12 2010 Subject: [Histonet] Great Histology Supervisor position in OK Message-ID: <292049750.1292010087069.JavaMail.cfservice@sl4app3> Hi Histonet Members, I am currently working on a Histology Supervisor position in OK. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From gupathlab <@t> gmail.com Sat Dec 11 10:21:48 2010 From: gupathlab <@t> gmail.com (lab operations) Date: Sat Dec 11 10:21:51 2010 Subject: [Histonet] Histology and cytology Employment Columbus OH Message-ID: Looking for experienced histology technicians and cytotechnologists in Columbus, Ohio for new GU pathology laboratory. Excellent compensation with great benefits. Experience with IHC, FISH, and GU pathology a plus. Send resume or CV to careers@aksm.com From tkngflght <@t> yahoo.com Sat Dec 11 13:11:49 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sat Dec 11 13:11:53 2010 Subject: [Histonet] Mohs tech needed in Houston ASAP Message-ID: <535442.38675.qm@web50907.mail.re2.yahoo.com> Hi Guys- ? I need at least one experienced Mohs tech for a three-room surgery practice.? Schedule is full days on Tuesdays and Thursdays.? Willing to work with more than one tech if you can only do one day during the week. ? Urgent need--please respond with a resume and any questions.? ? Cheryl Kerry, HT(ASCP) 281.883.7704 cell (leave a message if I don't pick up and I'll call you back) From akemiat3377 <@t> yahoo.com Sat Dec 11 15:52:07 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Sat Dec 11 15:52:12 2010 Subject: [Histonet] Available positions at NIH Message-ID: Hi fellow histoner's, I was contacted by a Linked In group member who is a HR recruiter at NIH. I am not looking for employment currently, but told him I would post this on the histonet for him. Below is the information he sent. We have some exciting new job opprotunities with our SoBran contracts at the National Institutes of Health, here in Bethesda, MD. Please take a moment and check out our website (www.sobran-inc.com click "careers") and see if you or anyone you know might be interested in having a discussion. In particular, we have QA Manager, BAS Asst, ABSL3 Tech Coordinator, Histotechologist positions that we are actively recruiting for and would greatly appreciate any leads If you know anyone in a role like those (or are looking to get into a role like that), please have them visit our website--you can actually fill out an application online. Feel free to forward this email along to your friends/coworkers/ contacts---thanks for the "pay it forward" networking :) Take Care, Page D. Page Bradham, PHR HR Mgr with SoBran / NIAID at NIH Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From SohrabB1 <@t> ah.org Fri Dec 17 09:47:45 2010 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Fri Dec 17 09:48:08 2010 Subject: [Histonet] No Mail?? Message-ID: <4D0B15A0.4347.0054.1@ah.org> Any Problem? I have not received any email from Histo-net last few days ?? Behnaz From JWeems <@t> sjha.org Fri Dec 17 09:54:07 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Dec 17 09:54:11 2010 Subject: [Histonet] No Mail?? In-Reply-To: <4D0B15A0.4347.0054.1@ah.org> References: <4D0B15A0.4347.0054.1@ah.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332488@CHEXCMS10.one.ads.che.org> And I kept getting a returned msg yesterday that I was trying to post. I am experiencing withdrawal!!! :>)j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, December 17, 2010 10:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No Mail?? Any Problem? I have not received any email from Histo-net last few days ?? Behnaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From JWeems <@t> sjha.org Fri Dec 17 10:10:13 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Dec 17 10:10:18 2010 Subject: [Histonet] Physician Signatures Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> Is everyone concerned about getting a physician's signature on requisitions, or do you have a fool proof plan in place? Inquiring minds want to know....... TIA! How many of you have electronic ordering for AP? This from CodeMap Compliance that I get.. Signatures Required on Laboratory Requisitions Although many professional groups and organizations lobbied against the signature requirement, CMS is implementing this provision as part of the CY 2011 MPFS. Starting January 1, 2011, the ordering physician must sign all written or paper requisitions for laboratory tests and services. Clinical laboratories will have to scramble to revise requisitions to include a signature line for the ordering physician. However, those labs that receive test orders via electronic or web-based interfaces should not be affected by the signature requirement included in the 2011 MPFS. In the comments to the proposed 2011 MPFS, CMS states, "Our proposed policy does not concern electronic or telephonic requests, because we do not consider these types of requests to be requisitions. As we discussed previously, a requisition is the actual paperwork, such as a form that is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Sandra.Harrison3 <@t> va.gov Fri Dec 17 10:29:23 2010 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Dec 17 10:29:28 2010 Subject: [Histonet] No Mail?? In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164044B332488@CHEXCMS10.one.ads.che.org> References: <4D0B15A0.4347.0054.1@ah.org> <92AD9B20A6C38C4587A9FEBE3A30E164044B332488@CHEXCMS10.one.ads.che.org> Message-ID: Wow...........didn't realize how addicted to Histonet I'd become till this week! Sandy Harrison Histology Supervisor VA Medical Center Minneapolis, MN 55417 612-467-2449 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 9:54 AM To: Behnaz Sohrab; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] No Mail?? And I kept getting a returned msg yesterday that I was trying to post. I am experiencing withdrawal!!! :>)j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, December 17, 2010 10:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No Mail?? Any Problem? I have not received any email from Histo-net last few days ?? Behnaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Fri Dec 17 10:33:38 2010 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Fri Dec 17 10:33:55 2010 Subject: [Histonet] Grossing Hood Velocity Requirements Message-ID: <8CD6C1F4AAAC78E-D9C-4CB9@webmail-m081.sysops.aol.com> I am looking to purchase a grossing hood and am in need of the requirements for fpm's (feet per minute). Can anyone tell me where to find them? Thank you, Ann From AHutton <@t> dh.org Fri Dec 17 10:32:47 2010 From: AHutton <@t> dh.org (Hutton, Allison) Date: Fri Dec 17 10:34:51 2010 Subject: [Histonet] job opening in PA Message-ID: <38A56C4F4630D348A50B3720409270870E0FE287@dhmail.dhorg.org> There is an open position for a full time day shift (variable start times) histology technician at Doylestown Hospital in Doylestown, PA. Position is responsible for all aspects of the histology department including, but not limited to, embedding, sectioning, staining (specials and IHC are manual), accessioning, and some clerical duties. We are a community hospital with volumes of approximately 10,500 cases per year with about half of the workload consisting of GI specimens. HT(ASCP) or eligible. Experience preferred. To apply: www.dh.org Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital 595 W. State St Doylestown, PA 18901 215-345-2264 ahutton@dh.org From mward <@t> wfubmc.edu Fri Dec 17 10:37:26 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Dec 17 10:37:47 2010 Subject: [Histonet] RE: Physician Signatures In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> Message-ID: Like everyone else we are struggling with how to get everyone to comply. If anyone has any plans I think the whole group could benefit. Right now our institution is getting together to figure out a way to get the information out to the surgeons, etc but no concrete plans. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Physician Signatures Is everyone concerned about getting a physician's signature on requisitions, or do you have a fool proof plan in place? Inquiring minds want to know....... TIA! How many of you have electronic ordering for AP? This from CodeMap Compliance that I get.. Signatures Required on Laboratory Requisitions Although many professional groups and organizations lobbied against the signature requirement, CMS is implementing this provision as part of the CY 2011 MPFS. Starting January 1, 2011, the ordering physician must sign all written or paper requisitions for laboratory tests and services. Clinical laboratories will have to scramble to revise requisitions to include a signature line for the ordering physician. However, those labs that receive test orders via electronic or web-based interfaces should not be affected by the signature requirement included in the 2011 MPFS. In the comments to the proposed 2011 MPFS, CMS states, "Our proposed policy does not concern electronic or telephonic requests, because we do not consider these types of requests to be requisitions. As we discussed previously, a requisition is the actual paperwork, such as a form that is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Fri Dec 17 10:46:36 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Dec 17 10:46:40 2010 Subject: [Histonet] Physician Signatures In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> Message-ID: I work in an integrated health system that has 9 Hospitals and 100's of outreach clients. All our hospitals are using Computerized Physician Order Entry (CPOE) for all tests ordered and our outreach clients use Electronic Order Entry via interface to their system or our web based system or they have client specific paper requisitions that have always required physician signature. We will be able to continue business as usual moving forward in 2011. The federal government specifically and definitely wants everyone to use electronic ordering and reporting processes so that we can move forward to the Electronic Medical Record (EMR) and reduce repeats and waste in laboratory tests. William DeSalvo, B.S., HTL(ASCP) > From: JWeems@sjha.org > To: histonet@lists.utsouthwestern.edu > Date: Fri, 17 Dec 2010 11:10:13 -0500 > Subject: [Histonet] Physician Signatures > > > Is everyone concerned about getting a physician's signature on requisitions, or do you have a fool proof plan in place? Inquiring minds want to know....... TIA! > > How many of you have electronic ordering for AP? > > This from CodeMap Compliance that I get.. > > Signatures Required on Laboratory Requisitions > > Although many professional groups and organizations lobbied against the signature requirement, CMS is implementing this provision as part of the CY 2011 MPFS. Starting January 1, 2011, the ordering physician must sign all written or paper requisitions for laboratory tests and services. Clinical laboratories will have to scramble to revise requisitions to include a signature line for the ordering physician. > > However, those labs that receive test orders via electronic or web-based interfaces should not be affected by the signature requirement included in the 2011 MPFS. In the comments to the proposed 2011 MPFS, CMS states, > > "Our proposed policy does not concern electronic or telephonic requests, because we do not consider these types of requests to be requisitions. As we discussed previously, a requisition is the actual paperwork, such as a form that is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient. > > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Dec 17 10:53:11 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Dec 17 10:53:17 2010 Subject: [Histonet] Physician Signatures In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324A4@CHEXCMS10.one.ads.che.org> It's wonderful that you are ahead of the game. I have electronic orders from GIDU and Breast Health, but haven't been able to get the ORs on board yet. I am hoping this will be the push they need to comply. Sure would make it easier! How does your order work? Our electronic order generates a paper requisition. - one in the lab and one to send with the specimen. Surgery says they don't have good access to printers, et. Do you have multiple frozens on complicated cases that require a requisition with one? And one they can write the special instructions on, etc? Thanks for your response. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax ________________________________ From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Friday, December 17, 2010 11:47 To: Weems, Joyce; histonet Subject: RE: [Histonet] Physician Signatures I work in an integrated health system that has 9 Hospitals and 100's of outreach clients. All our hospitals are using Computerized Physician Order Entry (CPOE) for all tests ordered and our outreach clients use Electronic Order Entry via interface to their system or our web based system or they have client specific paper requisitions that have always required physician signature. We will be able to continue business as usual moving forward in 2011. The federal government specifically and definitely wants everyone to use electronic ordering and reporting processes so that we can move forward to the Electronic Medical Record (EMR) and reduce repeats and waste in laboratory tests. William DeSalvo, B.S., HTL(ASCP) > From: JWeems@sjha.org > To: histonet@lists.utsouthwestern.edu > Date: Fri, 17 Dec 2010 11:10:13 -0500 > Subject: [Histonet] Physician Signatures > > > Is everyone concerned about getting a physician's signature on requisitions, or do you have a fool proof plan in place? Inquiring minds want to know....... TIA! > > How many of you have electronic ordering for AP? > > This from CodeMap Compliance that I get.. > > Signatures Required on Laboratory Requisitions > > Although many professional groups and organizations lobbied against the signature requirement, CMS is implementing this provision as part of the CY 2011 MPFS. Starting January 1, 2011, the ordering physician must sign all written or paper requisitions for laboratory tests and services. Clinical laboratories will have to scramble to revise requisitions to include a signature line for the ordering physician. > > However, those labs that receive test orders via electronic or web-based interfaces should not be affected by the signature requirement included in the 2011 MPFS. In the comments to the proposed 2011 MPFS, CMS states, > > "Our proposed policy does not concern electronic or telephonic requests, because we do not consider these types of requests to be requisitions. As we discussed previously, a requisition is the actual paperwork, such as a form that is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient. > > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From nancy_schmitt <@t> pa-ucl.com Fri Dec 17 11:31:19 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Dec 17 11:31:37 2010 Subject: [Histonet] Cassette/slide labelers - again Message-ID: <737BD0BF52F0744B96B74B61756AC06443B36D49E8@hestia.ad.pa-ucl.com> Sorry to boot this dog around a bit more, BUT - We have Cerner CoPath plus and Dragon speak and are looking at Thermo and General Data. Do they interface smoothly? Do your cassettes get printed at accession or grossing? Are you using multiple colors of cassettes? What are you doing for slides that require levels or a mix of non-charged and charged slides? Thank you for any and all input! Nancy Schmitt, MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From JWeems <@t> sjha.org Fri Dec 17 11:36:21 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Dec 17 11:36:27 2010 Subject: [Histonet] RE: Cassette/slide labelers - again In-Reply-To: <737BD0BF52F0744B96B74B61756AC06443B36D49E8@hestia.ad.pa-ucl.com> References: <737BD0BF52F0744B96B74B61756AC06443B36D49E8@hestia.ad.pa-ucl.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324C6@CHEXCMS10.one.ads.che.org> We have GE Ultra - formerly Triple G. Cassettes are printed on our Sakura printer at accessioning (number is attached to the specimen) and more printed at grossing if needed. We use multiple colors of cassettes - the color is tied to the specimen of can be changed for decal. Slides are printed on our Sakura slide printer in batch by case type. Charged slides on in one hopper and printing is directed there for the necessary printing through the soft ware. It was painful getting the interface done, but once it was, it has been a good thing. Good luck, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, December 17, 2010 12:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette/slide labelers - again Sorry to boot this dog around a bit more, BUT - We have Cerner CoPath plus and Dragon speak and are looking at Thermo and General Data. Do they interface smoothly? Do your cassettes get printed at accession or grossing? Are you using multiple colors of cassettes? What are you doing for slides that require levels or a mix of non-charged and charged slides? Thank you for any and all input! Nancy Schmitt, MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From trathborne <@t> somerset-healthcare.com Fri Dec 17 11:39:28 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Dec 17 11:40:58 2010 Subject: [Histonet] RE: Cassette/slide labelers - again In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324C6@CHEXCMS10.one.ads.che.org> Message-ID: Do you find that the number of errors has decreased since you have implemented this? Our pathologists are hesitant because they worry too many pre-printed cassettes could get mixed up. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 12:36 PM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette/slide labelers - again We have GE Ultra - formerly Triple G. Cassettes are printed on our Sakura printer at accessioning (number is attached to the specimen) and more printed at grossing if needed. We use multiple colors of cassettes - the color is tied to the specimen of can be changed for decal. Slides are printed on our Sakura slide printer in batch by case type. Charged slides on in one hopper and printing is directed there for the necessary printing through the soft ware. It was painful getting the interface done, but once it was, it has been a good thing. Good luck, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, December 17, 2010 12:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette/slide labelers - again Sorry to boot this dog around a bit more, BUT - We have Cerner CoPath plus and Dragon speak and are looking at Thermo and General Data. Do they interface smoothly? Do your cassettes get printed at accession or grossing? Are you using multiple colors of cassettes? What are you doing for slides that require levels or a mix of non-charged and charged slides? Thank you for any and all input! Nancy Schmitt, MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Bryan.Watson <@t> parkview.com Fri Dec 17 11:43:13 2010 From: Bryan.Watson <@t> parkview.com (Bryan Watson) Date: Fri Dec 17 11:43:25 2010 Subject: [Histonet] Cap guidelines Message-ID: Hey, I have a question. I was wondering if there are what the CAP regulations are for grossing, and if cutting bones for diagnosis falls into the same category as grossing other tissues. In out lab we have a PA who grosses all of the specimens. However the HT's cut down bone sections for bones that have avascular necrosis, fracture, etc. . . as the pre surgical diagnosis. I am trying to find out if this practice would be ok in the eyes of CAP or if the PA should be doing that as well. Thank you, Bryan HT (ASCP) From JWeems <@t> sjha.org Fri Dec 17 11:43:21 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Dec 17 11:43:29 2010 Subject: [Histonet] RE: Cassette/slide labelers - again In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324C6@CHEXCMS10.one.ads.che.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324CB@CHEXCMS10.one.ads.che.org> I don't have documentation to say there's a decrease or increase, but we don't find it to be a problem at all. The cassettes are put on top of each container and seem to live there. We do have them bar-coded and in our ideal world, we would check, but our system doesn't work all the way through yet! -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Friday, December 17, 2010 12:39 To: Weems, Joyce; Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cassette/slide labelers - again Do you find that the number of errors has decreased since you have implemented this? Our pathologists are hesitant because they worry too many pre-printed cassettes could get mixed up. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 12:36 PM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette/slide labelers - again We have GE Ultra - formerly Triple G. Cassettes are printed on our Sakura printer at accessioning (number is attached to the specimen) and more printed at grossing if needed. We use multiple colors of cassettes - the color is tied to the specimen of can be changed for decal. Slides are printed on our Sakura slide printer in batch by case type. Charged slides on in one hopper and printing is directed there for the necessary printing through the soft ware. It was painful getting the interface done, but once it was, it has been a good thing. Good luck, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, December 17, 2010 12:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette/slide labelers - again Sorry to boot this dog around a bit more, BUT - We have Cerner CoPath plus and Dragon speak and are looking at Thermo and General Data. Do they interface smoothly? Do your cassettes get printed at accession or grossing? Are you using multiple colors of cassettes? What are you doing for slides that require levels or a mix of non-charged and charged slides? Thank you for any and all input! Nancy Schmitt, MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From JEllin <@t> yumaregional.org Fri Dec 17 11:46:15 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Dec 17 11:45:38 2010 Subject: [Histonet] Physician Signatures In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324A4@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> <92AD9B20A6C38C4587A9FEBE3A30E164044B3324A4@CHEXCMS10.one.ads.che.org> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C6ADC@EXCHANGECLUSTER.yumaregional.local> This is true about electronic ordering but there is a provision on what is acceptable for electronic order, just ordering in a system and spitting out requisitions does not comply, There needs to be password verification and review of this information by the clinician before it is ordered and sent to the laboratory performing the service when the requisition is created. They go on to say that orders and requisitions are different aspects and should not be confused. IF the orders are just received through an electronic interface then there is a difference. But if the specimen is received using any paper form then this needs to be signed by the clinician either electronically or manually. We are looking at this really closely, with using paper requisitions, even orders from the OR for frozens, molecular studies, interoperative work, all needs to be signed by the surgeon. Can you imagine the impact that this is going to cause. Our current solutions is to have maybe standing orders in place and move and look at process within the Main OR's, outpatient OR and clients. Once again a huge problem,, but Bill is right this will lead to more and more paperless enviroments. Jesus Ellin Yuma Regional Medical Center ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From JWeems <@t> sjha.org Fri Dec 17 11:55:15 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Dec 17 11:55:20 2010 Subject: [Histonet] Physician Signatures In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8021C6ADC@EXCHANGECLUSTER.yumaregional.local> References: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> <92AD9B20A6C38C4587A9FEBE3A30E164044B3324A4@CHEXCMS10.one.ads.che.org> <29BE166A2CF48D459853F8EC57CD37E8021C6ADC@EXCHANGECLUSTER.yumaregional.local> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324D1@CHEXCMS10.one.ads.che.org> I had that concern about our electronic orders, because it certainly is just spitting out a paper. We must do something! How can we make CMS hear us? ASCP just send an email that a hospital is OR says it is unjust. j -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, December 17, 2010 12:46 To: Weems, Joyce; WILLIAM DESALVO; histonet Subject: RE: [Histonet] Physician Signatures This is true about electronic ordering but there is a provision on what is acceptable for electronic order, just ordering in a system and spitting out requisitions does not comply, There needs to be password verification and review of this information by the clinician before it is ordered and sent to the laboratory performing the service when the requisition is created. They go on to say that orders and requisitions are different aspects and should not be confused. IF the orders are just received through an electronic interface then there is a difference. But if the specimen is received using any paper form then this needs to be signed by the clinician either electronically or manually. We are looking at this really closely, with using paper requisitions, even orders from the OR for frozens, molecular studies, interoperative work, all needs to be signed by the surgeon. Can you imagine the impact that this is going to cause. Our current solutions is to have maybe standing orders in place and move and look at process within the Main OR's, outpatient OR and clients. Once again a huge problem,, but Bill is right this will lead to more and more paperless enviroments. Jesus Ellin Yuma Regional Medical Center ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From HornHV <@t> archildrens.org Fri Dec 17 12:04:24 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Dec 17 12:04:28 2010 Subject: [Histonet] RE: Physician Signatures In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> Message-ID: <25A4DE08332B19499904459F00AAACB7194516A5FF@EVS1.archildrens.org> We have the physicians sign the reqs. The surgeons sign them before the req goes to the chart and that copy is stored electronically in the EMR. We get a copy in our lab. From our clinics the docs sign the reqs in clinic. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Physician Signatures Is everyone concerned about getting a physician's signature on requisitions, or do you have a fool proof plan in place? Inquiring minds want to know....... TIA! How many of you have electronic ordering for AP? This from CodeMap Compliance that I get.. Signatures Required on Laboratory Requisitions Although many professional groups and organizations lobbied against the signature requirement, CMS is implementing this provision as part of the CY 2011 MPFS. Starting January 1, 2011, the ordering physician must sign all written or paper requisitions for laboratory tests and services. Clinical laboratories will have to scramble to revise requisitions to include a signature line for the ordering physician. However, those labs that receive test orders via electronic or web-based interfaces should not be affected by the signature requirement included in the 2011 MPFS. In the comments to the proposed 2011 MPFS, CMS states, "Our proposed policy does not concern electronic or telephonic requests, because we do not consider these types of requests to be requisitions. As we discussed previously, a requisition is the actual paperwork, such as a form that is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From wdesalvo.cac <@t> hotmail.com Fri Dec 17 12:55:40 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Dec 17 12:55:45 2010 Subject: [Histonet] Physician Signatures In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8021C6ADC@EXCHANGECLUSTER.yumaregional.local> References: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> <92AD9B20A6C38C4587A9FEBE3A30E164044B3324A4@CHEXCMS10.one.ads.che.org>, <29BE166A2CF48D459853F8EC57CD37E8021C6ADC@EXCHANGECLUSTER.yumaregional.local> Message-ID: I previously did not go into to deep detail about security and compliance, but in our system all computer portals are password protected, orders are signed with electronic signature of the ordering person and when a hard copy paper form is printed, the ordering physician's electronic signature is applied. It is the same type of process and signature that is used by the pathologist to electronically sign off all pathology reports. I absolutely agree that you must control access to ordering and maintain integrity in the system. As you work through your process to develop a more electronic system, you will have the opportunity to build in and require proper controls. With those controls implemented, you can then become confident that the electronic process can and will meet your regulatory requirements. William DeSalvo, B.S., HTL(ASCP) > Subject: RE: [Histonet] Physician Signatures > Date: Fri, 17 Dec 2010 10:46:15 -0700 > From: JEllin@yumaregional.org > To: JWeems@sjha.org; wdesalvo.cac@hotmail.com; histonet@lists.utsouthwestern.edu > > This is true about electronic ordering but there is a provision on what > is acceptable for electronic order, just ordering in a system and > spitting out requisitions does not comply, There needs to be password > verification and review of this information by the clinician before it > is ordered and sent to the laboratory performing the service when the > requisition is created. They go on to say that orders and requisitions > are different aspects and should not be confused. IF the orders are just > received through an electronic interface then there is a difference. > But if the specimen is received using any paper form then this needs to > be signed by the clinician either electronically or manually. > > We are looking at this really closely, with using paper requisitions, > even orders from the OR for frozens, molecular studies, interoperative > work, all needs to be signed by the surgeon. Can you imagine the impact > that this is going to cause. > > Our current solutions is to have maybe standing orders in place and move > and look at process within the Main OR's, outpatient OR and clients. > Once again a huge problem,, but Bill is right this will lead to more and > more paperless enviroments. > > > Jesus Ellin > Yuma Regional Medical Center > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ From trathborne <@t> somerset-healthcare.com Fri Dec 17 13:06:04 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Dec 17 13:06:55 2010 Subject: [Histonet] Physician Signatures In-Reply-To: Message-ID: So how do the surgeons electronically order a test? Do they order it before the procedure? What if they discover something and need to add an additional specimen? Our orders are electronically entered in the OR during the procedure. The surgeon is scrubbed and performing the procedure while the circulating nurse places the order. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of WILLIAM DESALVO Sent: Friday, December 17, 2010 1:56 PM To: Jesus Ellin; jweems@sjha.org; histonet Subject: RE: [Histonet] Physician Signatures I previously did not go into to deep detail about security and compliance, but in our system all computer portals are password protected, orders are signed with electronic signature of the ordering person and when a hard copy paper form is printed, the ordering physician's electronic signature is applied. It is the same type of process and signature that is used by the pathologist to electronically sign off all pathology reports. I absolutely agree that you must control access to ordering and maintain integrity in the system. As you work through your process to develop a more electronic system, you will have the opportunity to build in and require proper controls. With those controls implemented, you can then become confident that the electronic process can and will meet your regulatory requirements. William DeSalvo, B.S., HTL(ASCP) > Subject: RE: [Histonet] Physician Signatures > Date: Fri, 17 Dec 2010 10:46:15 -0700 > From: JEllin@yumaregional.org > To: JWeems@sjha.org; wdesalvo.cac@hotmail.com; histonet@lists.utsouthwestern.edu > > This is true about electronic ordering but there is a provision on what > is acceptable for electronic order, just ordering in a system and > spitting out requisitions does not comply, There needs to be password > verification and review of this information by the clinician before it > is ordered and sent to the laboratory performing the service when the > requisition is created. They go on to say that orders and requisitions > are different aspects and should not be confused. IF the orders are just > received through an electronic interface then there is a difference. > But if the specimen is received using any paper form then this needs to > be signed by the clinician either electronically or manually. > > We are looking at this really closely, with using paper requisitions, > even orders from the OR for frozens, molecular studies, interoperative > work, all needs to be signed by the surgeon. Can you imagine the impact > that this is going to cause. > > Our current solutions is to have maybe standing orders in place and move > and look at process within the Main OR's, outpatient OR and clients. > Once again a huge problem,, but Bill is right this will lead to more and > more paperless enviroments. > > > Jesus Ellin > Yuma Regional Medical Center > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From mike <@t> pathview.com Fri Dec 17 13:12:53 2010 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Dec 17 13:13:34 2010 Subject: [Histonet] RE: Cassette/slide labelers - again In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324C6@CHEXCMS10.one.ads.che.org> Message-ID: <003e01cb9e1e$713be7f0$53b3b7d0$@pathview.com> I'm sorry to say, but I think you're missing a major point here. Ideally, the cassettes should print when the specimen is scanned at the grossing station. Only the cassettes for the specific specimen should print. In that manner, there are no pre-printed cassettes and no cassettes wrongly associated with the specimen. Unfortunately, not all systems work that way. If your system does not work that way, then 'yes' there is still an advantage to using machine imprinted cassettes, but you're not getting ALL the benefits. I guess, I tend to agree with your pathologists. Pre-printed anything can get mixed up. If you have to do that, you need to have some sort of manual or automated double check. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, December 17, 2010 11:39 AM To: Weems, Joyce; Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cassette/slide labelers - again Do you find that the number of errors has decreased since you have implemented this? Our pathologists are hesitant because they worry too many pre-printed cassettes could get mixed up. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 12:36 PM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette/slide labelers - again We have GE Ultra - formerly Triple G. Cassettes are printed on our Sakura printer at accessioning (number is attached to the specimen) and more printed at grossing if needed. We use multiple colors of cassettes - the color is tied to the specimen of can be changed for decal. Slides are printed on our Sakura slide printer in batch by case type. Charged slides on in one hopper and printing is directed there for the necessary printing through the soft ware. It was painful getting the interface done, but once it was, it has been a good thing. Good luck, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, December 17, 2010 12:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette/slide labelers - again Sorry to boot this dog around a bit more, BUT - We have Cerner CoPath plus and Dragon speak and are looking at Thermo and General Data. Do they interface smoothly? Do your cassettes get printed at accession or grossing? Are you using multiple colors of cassettes? What are you doing for slides that require levels or a mix of non-charged and charged slides? Thank you for any and all input! Nancy Schmitt, MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Bill.Tench <@t> pph.org Fri Dec 17 13:19:57 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Fri Dec 17 13:20:02 2010 Subject: [Histonet] cassette labelers, etc Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5919@MAIL1.pph.local> The response by M. Mihalik nails the problem. Anything that is batched (cassette labeling, slide labeling, case labeling) is known to be high risk for mistakes even though it may seem to be more "efficient." On-demand-labeling is the key to significantly reducing identification errors. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From wdesalvo.cac <@t> hotmail.com Fri Dec 17 13:38:12 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Dec 17 13:38:17 2010 Subject: [Histonet] Physician Signatures In-Reply-To: References: , Message-ID: They sign into the case prior to surgery, they have Physician Assistant (PA) assigned to the case, a nurse is assigned to the case and they then enter additional information into the system after they complete surgery. It meets requirements. In Pathology, many of your Frozen Section specimens arrive in the lab on verbal orders or nursing applying a signature and we have processed for years. I believe the key point that you have to wrap your head around is that the electronic orders by the surgeon are complete before there is any delivery of results, the signed and preliminary or final report by the pathologist and that meets regulatory requirements. I have more concern on the clinical side. Orders can be entered, tests performed and results delivered more easily than in Pathology. William DeSalvo, B.S., HTL(ASCP) > Subject: RE: [Histonet] Physician Signatures > Date: Fri, 17 Dec 2010 14:06:04 -0500 > From: trathborne@somerset-healthcare.com > To: wdesalvo.cac@hotmail.com; jellin@yumaregional.org; jweems@sjha.org; histonet@lists.utsouthwestern.edu > > So how do the surgeons electronically order a test? Do they order it before the procedure? What if they discover something and need to add an additional specimen? Our orders are electronically entered in the OR during the procedure. The surgeon is scrubbed and performing the procedure while the circulating nurse places the order. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of WILLIAM > DESALVO > Sent: Friday, December 17, 2010 1:56 PM > To: Jesus Ellin; jweems@sjha.org; histonet > Subject: RE: [Histonet] Physician Signatures > > > > I previously did not go into to deep detail about security and compliance, but in our system all computer portals are password protected, orders are signed with electronic signature of the ordering person and when a hard copy paper form is printed, the ordering physician's electronic signature is applied. It is the same type of process and signature that is used by the pathologist to electronically sign off all pathology reports. I absolutely agree that you must control access to ordering and maintain integrity in the system. As you work through your process to develop a more electronic system, you will have the opportunity to build in and require proper controls. With those controls implemented, you can then become confident that the electronic process can and will meet your regulatory requirements. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > Subject: RE: [Histonet] Physician Signatures > > Date: Fri, 17 Dec 2010 10:46:15 -0700 > > From: JEllin@yumaregional.org > > To: JWeems@sjha.org; wdesalvo.cac@hotmail.com; histonet@lists.utsouthwestern.edu > > > > This is true about electronic ordering but there is a provision on what > > is acceptable for electronic order, just ordering in a system and > > spitting out requisitions does not comply, There needs to be password > > verification and review of this information by the clinician before it > > is ordered and sent to the laboratory performing the service when the > > requisition is created. They go on to say that orders and requisitions > > are different aspects and should not be confused. IF the orders are just > > received through an electronic interface then there is a difference. > > But if the specimen is received using any paper form then this needs to > > be signed by the clinician either electronically or manually. > > > > We are looking at this really closely, with using paper requisitions, > > even orders from the OR for frozens, molecular studies, interoperative > > work, all needs to be signed by the surgeon. Can you imagine the impact > > that this is going to cause. > > > > Our current solutions is to have maybe standing orders in place and move > > and look at process within the Main OR's, outpatient OR and clients. > > Once again a huge problem,, but Bill is right this will lead to more and > > more paperless enviroments. > > > > > > Jesus Ellin > > Yuma Regional Medical Center > > > > ______________________________________________________________________ > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged > > or exempt from disclosure under applicable law. If you are > > not the intended recipient(s), you are notified that the > > dissemination, distribution, or copying of this message is > > strictly prohibited. If you receive this message in error, > > or are not the named recipient(s), please notify the sender > > at either the e-mail, fax, address, or telephone number > > listed above and delete this e-mail from your computer. > > Thank You. > > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. From k84as <@t> yahoo.com Fri Dec 17 14:40:34 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Dec 17 14:40:38 2010 Subject: [Histonet] elecron microscope needed Message-ID: <788621.16730.qm@web112610.mail.gq1.yahoo.com> hi all i miss you several days. we are medium research lab and looking forward to bring scanning & transmission electron microscope . so which manufacture or brand and model you recommend us and average price ?? any offers are welcomed to my mail? ? thanx in advance ? mohamed From sfeher <@t> CMC-NH.ORG Fri Dec 17 15:05:19 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Dec 17 15:05:23 2010 Subject: [Histonet] Grossing Hood Velocity Requirements In-Reply-To: <8CD6C1F4AAAC78E-D9C-4CB9@webmail-m081.sysops.aol.com> References: <8CD6C1F4AAAC78E-D9C-4CB9@webmail-m081.sysops.aol.com> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC24386AEE@exchange.cmc-nh.org> We purchased Thermo's Gross Lab Senior grossing stations. A rule of thumb is for these to pull at 450-500 CFM. If you purchase the hoods that include the fan motor, these are factory set to this. We hooked ours in to the hospital ventilation system so it was a bit trickier to achieve this velocity. We currently are pulling at 550 CFM per hood. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com Sent: Friday, December 17, 2010 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Hood Velocity Requirements I am looking to purchase a grossing hood and am in need of the requirements for fpm's (feet per minute). Can anyone tell me where to find them? Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Fri Dec 17 15:21:57 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Fri Dec 17 15:22:21 2010 Subject: [Histonet] Grossing Hood Velocity Requirements In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC24386AEE@exchange.cmc-nh.org> References: <8CD6C1F4AAAC78E-D9C-4CB9@webmail-m081.sysops.aol.com> <0908FC0A43B87A4FB60EDCCA06AABC24386AEE@exchange.cmc-nh.org> Message-ID: You should call Tony from Mopec... we got ours from him and he goes the extra mile... He was there at installation and did a few separate in-service training. He and his engineers even customized the CFM to give us greater suction! www.MOPEC.com Tony Carrillo (MOPEC) ABM57@hotmail.com 1-951-377-5955 1-800-377-5395 Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Friday, December 17, 2010 1:05 PM To: thisisann@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Grossing Hood Velocity Requirements We purchased Thermo's Gross Lab Senior grossing stations. A rule of thumb is for these to pull at 450-500 CFM. If you purchase the hoods that include the fan motor, these are factory set to this. We hooked ours in to the hospital ventilation system so it was a bit trickier to achieve this velocity. We currently are pulling at 550 CFM per hood. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com Sent: Friday, December 17, 2010 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Hood Velocity Requirements I am looking to purchase a grossing hood and am in need of the requirements for fpm's (feet per minute). Can anyone tell me where to find them? Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Laura.Miller <@t> leica-microsystems.com Fri Dec 17 15:49:54 2010 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Fri Dec 17 17:44:42 2010 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 12/17/2010 and will not return until 12/28/2010. I will be out of the country and I will have limited access to emails, I will try my best to answer as soon as possible. Have a safe and merry holiday season!!! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From histotech <@t> imagesbyhopper.com Fri Dec 17 19:27:10 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Dec 17 19:28:20 2010 Subject: [Histonet] No Mail?? In-Reply-To: References: <4D0B15A0.4347.0054.1@ah.org> <92AD9B20A6C38C4587A9FEBE3A30E164044B332488@CHEXCMS10.one.ads.che.org> Message-ID: <6A35793E-C2E9-45A3-A85E-16F281D32B24@imagesbyhopper.com> Oh man! The first time Histonet stopped working, I thought it was my email... but this time I knew better! And I too didn't realize how much I looked forward to the messages/thoughts/questions shared on this board. So histonet, what's been causing the problem? Michelle On Dec 17, 2010, at 11:29 AM, "Harrison, Sandra C." wrote: > Wow...........didn't realize how addicted to Histonet I'd become till > this week! > > Sandy Harrison > Histology Supervisor > VA Medical Center > Minneapolis, MN 55417 > 612-467-2449 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Friday, December 17, 2010 9:54 AM > To: Behnaz Sohrab; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] No Mail?? > > And I kept getting a returned msg yesterday that I was trying to post. I > am experiencing withdrawal!!! :>)j > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz > Sohrab > Sent: Friday, December 17, 2010 10:48 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] No Mail?? > > Any Problem? I have not received any email from Histo-net last few days > ?? > Behnaz > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sadey <@t> hotmail.ca Sat Dec 18 06:06:47 2010 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Sat Dec 18 06:06:52 2010 Subject: [Histonet] (no subject) Message-ID: Hello, Can anyone tell me if Dako still supports IHC's on the Artisan? If so, can it do a Pin 4? Thanks in advance. From lpwenk <@t> sbcglobal.net Sat Dec 18 06:26:20 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Dec 18 06:26:22 2010 Subject: [Histonet] EM cacodylate Message-ID: <55E1BC6D06D045AE919C3B31B4EBD284@HP2010> Need some help from Electron Microscopy people. What is the best substitute for cacodylate buffer (arsenic) when using on kidney and nerve biopsies? Least amount of protein clumping, little to now salt precipitate, good EM resolution? Thanks in advance. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From kathyenelson <@t> hotmail.com Sun Dec 19 00:25:54 2010 From: kathyenelson <@t> hotmail.com (Kathy Nelson) Date: Sun Dec 19 00:25:59 2010 Subject: [Histonet] Help Message-ID: Solutions for cracks in tissue microscopically esp. in tumors and BCC specimens. Thanks kathyenelson@hotmail.com From saby_joseph_a <@t> yahoo.com Sun Dec 19 10:09:32 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Sun Dec 19 10:09:38 2010 Subject: [Histonet] Help In-Reply-To: References: Message-ID: <178486.31609.qm@web114419.mail.gq1.yahoo.com> Kathy- What do these cracks look like?? Are they arranged in a parallel manner?? Or do they have the appearance of the cracks seen in dry mud? Parallel aligned cracks are often found in overprocessed small biospies.? These small samples become hard and brittle.? The impact of the tissue on the microtome blade during aggressive facing force these cracks deep into the tissue.? With care, patience and some luck you may be able to get beyond these cracks by soaking the blocks, facing with thin sections on a repeated basis.? What will determine whether you are successful will be whether there is enough depth to your biopsy to allow you to get beyond the facing artifact. If the cracks resemble dry earth, then we are looking at a fixation/processing issue.? These cracks do not appear in the tissue until the xylenes after slide staining.? If the tissues are not well fixed, then processing reagents will not be able to fully penetrate the tissues.? If possible,?I would suggest you perform retrims on your tissue and process them normally.? They should have had enough time to fix when you do your retrims, and they should be fine.? In a hospital setting, this may not be possible.? You can deparaffinze and rehydrate your tissue samples by running them through your processor's cleaning program.? Place them back in fixative for a while, then reprocess them.? I have also seen this artifcat in tissue when (due to a processor malfunction) tissue samples were exposed to high heat during processing.? Look for blood cells being "laked" in the larger blood vessels (blood cells look like a homogenous mass rather than being able to cell boundaries).? Sometimes there will be small round black precitate granules over the affected tissue areas.? You will need to determine the best course depending on the extent of the damage.? Extensive damage will probaly require retrims.? I hope this helps.? Please get back with me if yoiu have any further questions. Joe Saby, BA HT 37 years in histotechnology ________________________________ From: Kathy Nelson To: Histonet Sent: Sun, December 19, 2010 1:25:54 AM Subject: [Histonet] Help Solutions for cracks in tissue microscopically esp. in tumors and BCC specimens. Thanks kathyenelson@hotmail.com ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Dec 19 17:21:12 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Dec 19 17:21:55 2010 Subject: SPAM-LOW: [Histonet] (no subject) In-Reply-To: References: Message-ID: <7AC4D242C02A4CDEB3B188062E8080C8@prueggihctechlt> I thought the artisan was a special stains instrument not IHC. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Saturday, December 18, 2010 5:07 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] (no subject) Hello, Can anyone tell me if Dako still supports IHC's on the Artisan? If so, can it do a Pin 4? Thanks in advance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Mon Dec 20 07:31:11 2010 From: mw <@t> personifysearch.com (Matt Ward) Date: Mon Dec 20 07:31:15 2010 Subject: [Histonet] Histology Training Specialist Located in the Chicago Area Message-ID: <50a35554603011af32eec4a116abaae0@mail.gmail.com> Good Morning, One of our leading clients is looking for a Histology Training Specialist to work out of their corporate office in the Chicago area. Please contact me directly at, mw@personifysearch.com, or at 800.875.6188 ext. 103, to learn more. The position offers a very competitive compensation package and great career growth. Thanks! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com *Histology Training Specialist * *The Company:* Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. *The Opportunity:* The company currently has an opening for a Histology Training Specialist. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Base: Depends on Experience Other: Full benefits - 401k program/matching *Primary Responsibilities:* The primary responsibility of this role will be to provide technical phone support by answering questions, troubleshooting problems, logging and closing complaint files and escalating major issues to appropriate company personnel. This role will also provide technical training on specified products in the company's newly constructed state-of-the-art Customer Support Laboratory. Training programs are designed for small groups to ensure maximum customer learning and satisfaction. Additional Responsibilities: - Provide product and applications phone support to end-users, field personnel and dealers for all product lines - Log all calls into Customer Support Database - Participate in development of training materials and conduct classes and labs for customers, employees and others as needed - Serve as technical liaison to Customer Service/Field Service/Product Management departments *Education and Experience Required:* Ability to interact with various people in a calm and positive fashion and the ability to effectively communicate information to groups of participants is required. Experience with data entry, MS Office programs (PowerPoint, Lotus Notes, Word) is also required. HT/HTL/QIHC (ASCP) is helpful but not required. From Ronald.Houston <@t> nationwidechildrens.org Mon Dec 20 08:00:25 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Dec 20 08:00:34 2010 Subject: SPAM-LOW: [Histonet] (no subject) In-Reply-To: <7AC4D242C02A4CDEB3B188062E8080C8@prueggihctechlt> References: <7AC4D242C02A4CDEB3B188062E8080C8@prueggihctechlt> Message-ID: Patsy, It does have the capability to do IHC, but I think I had heard that Dako is no longer supporting this function (could be wrong though) Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Sunday, December 19, 2010 6:21 PM To: 'Sheila Adey'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] (no subject) I thought the artisan was a special stains instrument not IHC. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Saturday, December 18, 2010 5:07 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] (no subject) Hello, Can anyone tell me if Dako still supports IHC's on the Artisan? If so, can it do a Pin 4? Thanks in advance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From tahseen <@t> brain.net.pk Mon Dec 20 08:49:25 2010 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Mon Dec 20 08:49:32 2010 Subject: [Histonet] comparisons between Dako,Lieca,seroTec and CellMarque Message-ID: <28745.203.135.35.66.1292856565.squirrel@brain.net.pk> Dear All, We are using Dako antibodies and EnVision kit for IHC on paraffin sections from 15 years there are no problem with us. Now our MMD required comparisons between other companies like Lieca,Cell Marque and SeroTec. Would any one like to advise me? Muhammad Tahseen Shaukat Khanum Memorial Cancer Hospital & RC Lahore Pakistan. From Timothy.Morken <@t> ucsfmedctr.org Mon Dec 20 10:03:08 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Mon Dec 20 10:03:32 2010 Subject: [Histonet] EM cacodylate In-Reply-To: <55E1BC6D06D045AE919C3B31B4EBD284@HP2010> References: <55E1BC6D06D045AE919C3B31B4EBD284@HP2010> Message-ID: Peggy, Carson's modification of Millonig's buffer with formalin is great for kidney and any other general EM use for general pathology labs. I used it for many, many years when I worked in EM and it worked just as well as the specialized EM glutaraldehyde fixatives / buffers. Just look up "Carson millonig" and you'll find several commercial sources. Or, of course, look at Carson's book for the formula. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Saturday, December 18, 2010 4:26 AM To: Histonet Subject: [Histonet] EM cacodylate Need some help from Electron Microscopy people. What is the best substitute for cacodylate buffer (arsenic) when using on kidney and nerve biopsies? Least amount of protein clumping, little to now salt precipitate, good EM resolution? Thanks in advance. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Mon Dec 20 10:22:05 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Mon Dec 20 10:21:01 2010 Subject: [Histonet] Great Dermpath Lab Management Position in NY Message-ID: <55809412.1292862125070.JavaMail.cfservice@SL4APP1> Hi Histonet Members, I am currently working on a Laboratory Manager position in NY. the ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in dermatopathology. This job opportunity is with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From relia1 <@t> earthlink.net Mon Dec 20 10:26:00 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Dec 20 10:26:03 2010 Subject: [Histonet] RELIA Histology Job Alert 12/20/2010 Permanent Full time Dayshift position in Austin, TX Message-ID: <930B2DD4E6C5471D8F490699FC3BEC70@ownerf1abaad51> Hi Histonetters!! I hope everybody is having a great day and enjoying the holiday season. I have a new opportunity and I want to put the word out. I am working with a grea client of mine located in Austin, TX and they are in need of a permanent full time dayshift ASCP or eligible histotech. They need someone with at least 2 years of experience. My client offers excellent compensation, benefits and relocation assistance. They also offer stability, security and opportunity for advancement not to mention a great group of people and an excellent supervisor to work with. If you or anyone you know might be interested in taking a look at this opportunity either now or after the holidays please let me know. I can be reached toll free at 866-607-3542 or on my cell at 407-353-5070 or e-mail relia1@earthlink.net Thanks-Pam Happy Holidays!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From JWeems <@t> sjha.org Mon Dec 20 10:35:40 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Dec 20 10:35:46 2010 Subject: SPAM-LOW: [Histonet] (no subject) In-Reply-To: <7AC4D242C02A4CDEB3B188062E8080C8@prueggihctechlt> References: <7AC4D242C02A4CDEB3B188062E8080C8@prueggihctechlt> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B33266F@CHEXCMS10.one.ads.che.org> It started out as both, but I understand that DAKO is phasing out the support of IHC on the Artisan. We have it, but never used it for IHC. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Sunday, December 19, 2010 18:21 To: 'Sheila Adey'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] (no subject) I thought the artisan was a special stains instrument not IHC. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Saturday, December 18, 2010 5:07 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] (no subject) Hello, Can anyone tell me if Dako still supports IHC's on the Artisan? If so, can it do a Pin 4? Thanks in advance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From ree3 <@t> leicester.ac.uk Mon Dec 20 10:55:57 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Mon Dec 20 11:00:58 2010 Subject: [Histonet] Grocott stain kit Message-ID: <7722595275A4DD4FA225B92CDBF174A1012CD07AD669@EXC-MBX3.cfs.le.ac.uk> I am after a staining kit for the Grocott methenamine silver method for fungi, any recommendations out there?,any opinions of the Grocott method versus the PAS method?,many thanks. Cheers Richard Edwards University of Leicester From victoria.spoon <@t> bassett.org Mon Dec 20 11:06:38 2010 From: victoria.spoon <@t> bassett.org (Spoon, Victoria) Date: Mon Dec 20 11:06:43 2010 Subject: [Histonet] vip 3000 tissue teck tissue processor Message-ID: Is anyone interested in purchasing a vip 3000 tissue tek tissue processor. It runs fine. Vendors welcome to contact me. Victoria Spoon Anatomic Pathology Manager Bassett Medical Center Cooperstown NY 13326 victoria.spoon@bassett.org Tel(607) 547-6357 Fax(607) 547-3203 From w_alkadhumi <@t> yahoo.com Mon Dec 20 11:43:02 2010 From: w_alkadhumi <@t> yahoo.com (wassan alkadhumi) Date: Mon Dec 20 11:43:07 2010 Subject: [Histonet] need ur help Message-ID: <517559.68856.qm@web45209.mail.sp1.yahoo.com> Hi all Hope u r?all doing well, am an?Iraqi histotech working in both histopathology?and IHC department?in Iraq.?One part of what i do is IHC (manual)?i have no problem with,?the other part is what?i have problems with, Renals and Livers,?we do regular?H?&E stain?, PAS and Masson Tirchrome?and all of them?were text book quality until ten days ago, the problem is?the Stains even the H&E are not Fine, its smugish. And?the most important stain? for the histopathologist is the Trichrome?which it should be fine and delacate and its not.?I checked the procedures and stain preparations?and i doing it the correctly ,?I made new solutions and?did?prolonged deparaffinization 10 min each step (3 xylenes,??2?100%ETOH,??one 90%ETOH,?one?70%ETOH) and there was?no difference.?I should?mention that?those problems happened with only Renal and Liver biopsies the H&E and PAS on other types of tissues are working just great.?the?one thing that?changed before 10 days ago?is the wether it became really cold and our lab temp. is not controlled.??Am going crazy please help me Thank u a lot Wassan Histotech. Shorsh hospital From JWeems <@t> sjha.org Mon Dec 20 12:03:04 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Dec 20 12:03:11 2010 Subject: [Histonet] need ur help In-Reply-To: <517559.68856.qm@web45209.mail.sp1.yahoo.com> References: <517559.68856.qm@web45209.mail.sp1.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B3326C9@CHEXCMS10.one.ads.che.org> Could something have happened with the fixative for these two tissues? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wassan alkadhumi Sent: Monday, December 20, 2010 12:43 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need ur help Hi all Hope u r?all doing well, am an?Iraqi histotech working in both histopathology?and IHC department?in Iraq.?One part of what i do is IHC (manual)?i have no problem with,?the other part is what?i have problems with, Renals and Livers,?we do regular?H?&E stain?, PAS and Masson Tirchrome?and all of them?were text book quality until ten days ago, the problem is?the Stains even the H&E are not Fine, its smugish. And?the most important stain? for the histopathologist is the Trichrome?which it should be fine and delacate and its not.?I checked the procedures and stain preparations?and i doing it the correctly ,?I made new solutions and?did?prolonged deparaffinization 10 min each step (3 xylenes,??2?100%ETOH,??one 90%ETOH,?one?70%ETOH) and there was?no difference.?I should?mention that?those problems happened with only Renal and Liver biopsies the H&E and PAS on other types of tissues are working just great.?the?one thing that?changed before 10 days ago?is the wether it became really cold and our lab temp. is not controlled.??Am going crazy please help me Thank u a lot Wassan Histotech. Shorsh hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From john <@t> imebinc.com Mon Dec 20 12:21:50 2010 From: john <@t> imebinc.com (john@imebinc.com) Date: Mon Dec 20 12:21:53 2010 Subject: [Histonet] RE: Histonet Digest, Vol 85, Issue 13/united clinnical labs Message-ID: Margaret , Do you have United Clinical labs in our data base? It a note#11 on cassette markers John -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 85, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. No Mail?? (Behnaz Sohrab) 2. RE: No Mail?? (Weems, Joyce) 3. Physician Signatures (Weems, Joyce) 4. RE: No Mail?? (Harrison, Sandra C.) 5. Grossing Hood Velocity Requirements (thisisann@aol.com) 6. job opening in PA (Hutton, Allison) 7. RE: Physician Signatures (Martha Ward) 8. RE: Physician Signatures (WILLIAM DESALVO) 9. RE: Physician Signatures (Weems, Joyce) 10. Cassette/slide labelers - again (Nancy Schmitt) 11. RE: Cassette/slide labelers - again (Weems, Joyce) 12. RE: RE: Cassette/slide labelers - again (Rathborne, Toni) 13. Cap guidelines (Bryan Watson) 14. RE: RE: Cassette/slide labelers - again (Weems, Joyce) 15. RE: Physician Signatures (Jesus Ellin) 16. RE: Physician Signatures (Weems, Joyce) ---------------------------------------------------------------------- Message: 1 Date: Fri, 17 Dec 2010 07:47:45 -0800 From: "Behnaz Sohrab" Subject: [Histonet] No Mail?? To: Message-ID: <4D0B15A0.4347.0054.1@ah.org> Content-Type: text/plain; charset=US-ASCII Any Problem? I have not received any email from Histo-net last few days ?? Behnaz ------------------------------ Message: 2 Date: Fri, 17 Dec 2010 10:54:07 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] No Mail?? To: Behnaz Sohrab , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332488@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" And I kept getting a returned msg yesterday that I was trying to post. I am experiencing withdrawal!!! :>)j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, December 17, 2010 10:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No Mail?? Any Problem? I have not received any email from Histo-net last few days ?? Behnaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Fri, 17 Dec 2010 11:10:13 -0500 From: "Weems, Joyce" Subject: [Histonet] Physician Signatures To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332492@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Is everyone concerned about getting a physician's signature on requisitions, or do you have a fool proof plan in place? Inquiring minds want to know....... TIA! How many of you have electronic ordering for AP? This from CodeMap Compliance that I get.. Signatures Required on Laboratory Requisitions Although many professional groups and organizations lobbied against the signature requirement, CMS is implementing this provision as part of the CY 2011 MPFS. Starting January 1, 2011, the ordering physician must sign all written or paper requisitions for laboratory tests and services. Clinical laboratories will have to scramble to revise requisitions to include a signature line for the ordering physician. However, those labs that receive test orders via electronic or web-based interfaces should not be affected by the signature requirement included in the 2011 MPFS. In the comments to the proposed 2011 MPFS, CMS states, "Our proposed policy does not concern electronic or telephonic requests, because we do not consider these types of requests to be requisitions. As we discussed previously, a requisition is the actual paperwork, such as a form that is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Fri, 17 Dec 2010 10:29:23 -0600 From: "Harrison, Sandra C." Subject: RE: [Histonet] No Mail?? To: "Weems, Joyce" , Message-ID: Content-Type: text/plain; charset="us-ascii" Wow...........didn't realize how addicted to Histonet I'd become till this week! Sandy Harrison Histology Supervisor VA Medical Center Minneapolis, MN 55417 612-467-2449 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 9:54 AM To: Behnaz Sohrab; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] No Mail?? And I kept getting a returned msg yesterday that I was trying to post. I am experiencing withdrawal!!! :>)j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, December 17, 2010 10:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No Mail?? Any Problem? I have not received any email from Histo-net last few days ?? Behnaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 17 Dec 2010 11:33:38 -0500 From: thisisann@aol.com Subject: [Histonet] Grossing Hood Velocity Requirements To: histonet@lists.utsouthwestern.edu Message-ID: <8CD6C1F4AAAC78E-D9C-4CB9@webmail-m081.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I am looking to purchase a grossing hood and am in need of the requirements for fpm's (feet per minute). Can anyone tell me where to find them? Thank you, Ann ------------------------------ Message: 6 Date: Fri, 17 Dec 2010 11:32:47 -0500 From: "Hutton, Allison" Subject: [Histonet] job opening in PA To: Message-ID: <38A56C4F4630D348A50B3720409270870E0FE287@dhmail.dhorg.org> Content-Type: text/plain; charset="iso-8859-1" There is an open position for a full time day shift (variable start times) histology technician at Doylestown Hospital in Doylestown, PA. Position is responsible for all aspects of the histology department including, but not limited to, embedding, sectioning, staining (specials and IHC are manual), accessioning, and some clerical duties. We are a community hospital with volumes of approximately 10,500 cases per year with about half of the workload consisting of GI specimens. HT(ASCP) or eligible. Experience preferred. To apply: www.dh.org Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital 595 W. State St Doylestown, PA 18901 215-345-2264 ahutton@dh.org ------------------------------ Message: 7 Date: Fri, 17 Dec 2010 16:37:26 +0000 From: Martha Ward Subject: [Histonet] RE: Physician Signatures To: "'Weems, Joyce'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Like everyone else we are struggling with how to get everyone to comply. If anyone has any plans I think the whole group could benefit. Right now our institution is getting together to figure out a way to get the information out to the surgeons, etc but no concrete plans. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Physician Signatures Is everyone concerned about getting a physician's signature on requisitions, or do you have a fool proof plan in place? Inquiring minds want to know....... TIA! How many of you have electronic ordering for AP? This from CodeMap Compliance that I get.. Signatures Required on Laboratory Requisitions Although many professional groups and organizations lobbied against the signature requirement, CMS is implementing this provision as part of the CY 2011 MPFS. Starting January 1, 2011, the ordering physician must sign all written or paper requisitions for laboratory tests and services. Clinical laboratories will have to scramble to revise requisitions to include a signature line for the ordering physician. However, those labs that receive test orders via electronic or web-based interfaces should not be affected by the signature requirement included in the 2011 MPFS. In the comments to the proposed 2011 MPFS, CMS states, "Our proposed policy does not concern electronic or telephonic requests, because we do not consider these types of requests to be requisitions. As we discussed previously, a requisition is the actual paperwork, such as a form that is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 17 Dec 2010 09:46:36 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] Physician Signatures To: , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I work in an integrated health system that has 9 Hospitals and 100's of outreach clients. All our hospitals are using Computerized Physician Order Entry (CPOE) for all tests ordered and our outreach clients use Electronic Order Entry via interface to their system or our web based system or they have client specific paper requisitions that have always required physician signature. We will be able to continue business as usual moving forward in 2011. The federal government specifically and definitely wants everyone to use electronic ordering and reporting processes so that we can move forward to the Electronic Medical Record (EMR) and reduce repeats and waste in laboratory tests. William DeSalvo, B.S., HTL(ASCP) > From: JWeems@sjha.org > To: histonet@lists.utsouthwestern.edu > Date: Fri, 17 Dec 2010 11:10:13 -0500 > Subject: [Histonet] Physician Signatures > > > Is everyone concerned about getting a physician's signature on requisitions, or do you have a fool proof plan in place? Inquiring minds want to know....... TIA! > > How many of you have electronic ordering for AP? > > This from CodeMap Compliance that I get.. > > Signatures Required on Laboratory Requisitions > > Although many professional groups and organizations lobbied against the signature requirement, CMS is implementing this provision as part of the CY 2011 MPFS. Starting January 1, 2011, the ordering physician must sign all written or paper requisitions for laboratory tests and services. Clinical laboratories will have to scramble to revise requisitions to include a signature line for the ordering physician. > > However, those labs that receive test orders via electronic or > web-based interfaces should not be affected by the signature > requirement included in the 2011 MPFS. In the comments to the proposed > 2011 MPFS, CMS states, > > "Our proposed policy does not concern electronic or telephonic requests, because we do not consider these types of requests to be requisitions. As we discussed previously, a requisition is the actual paperwork, such as a form that is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient. > > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 17 Dec 2010 11:53:11 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] Physician Signatures To: WILLIAM DESALVO , histonet Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324A4@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" It's wonderful that you are ahead of the game. I have electronic orders from GIDU and Breast Health, but haven't been able to get the ORs on board yet. I am hoping this will be the push they need to comply. Sure would make it easier! How does your order work? Our electronic order generates a paper requisition. - one in the lab and one to send with the specimen. Surgery says they don't have good access to printers, et. Do you have multiple frozens on complicated cases that require a requisition with one? And one they can write the special instructions on, etc? Thanks for your response. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax ________________________________ From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Friday, December 17, 2010 11:47 To: Weems, Joyce; histonet Subject: RE: [Histonet] Physician Signatures I work in an integrated health system that has 9 Hospitals and 100's of outreach clients. All our hospitals are using Computerized Physician Order Entry (CPOE) for all tests ordered and our outreach clients use Electronic Order Entry via interface to their system or our web based system or they have client specific paper requisitions that have always required physician signature. We will be able to continue business as usual moving forward in 2011. The federal government specifically and definitely wants everyone to use electronic ordering and reporting processes so that we can move forward to the Electronic Medical Record (EMR) and reduce repeats and waste in laboratory tests. William DeSalvo, B.S., HTL(ASCP) > From: JWeems@sjha.org > To: histonet@lists.utsouthwestern.edu > Date: Fri, 17 Dec 2010 11:10:13 -0500 > Subject: [Histonet] Physician Signatures > > > Is everyone concerned about getting a physician's signature on requisitions, or do you have a fool proof plan in place? Inquiring minds want to know....... TIA! > > How many of you have electronic ordering for AP? > > This from CodeMap Compliance that I get.. > > Signatures Required on Laboratory Requisitions > > Although many professional groups and organizations lobbied against the signature requirement, CMS is implementing this provision as part of the CY 2011 MPFS. Starting January 1, 2011, the ordering physician must sign all written or paper requisitions for laboratory tests and services. Clinical laboratories will have to scramble to revise requisitions to include a signature line for the ordering physician. > > However, those labs that receive test orders via electronic or > web-based interfaces should not be affected by the signature > requirement included in the 2011 MPFS. In the comments to the proposed > 2011 MPFS, CMS states, > > "Our proposed policy does not concern electronic or telephonic requests, because we do not consider these types of requests to be requisitions. As we discussed previously, a requisition is the actual paperwork, such as a form that is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient. > > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 10 Date: Fri, 17 Dec 2010 11:31:19 -0600 From: Nancy Schmitt Subject: [Histonet] Cassette/slide labelers - again To: "histonet@lists.utsouthwestern.edu" Message-ID: <737BD0BF52F0744B96B74B61756AC06443B36D49E8@hestia.ad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Sorry to boot this dog around a bit more, BUT - We have Cerner CoPath plus and Dragon speak and are looking at Thermo and General Data. Do they interface smoothly? Do your cassettes get printed at accession or grossing? Are you using multiple colors of cassettes? What are you doing for slides that require levels or a mix of non-charged and charged slides? Thank you for any and all input! Nancy Schmitt, MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 11 Date: Fri, 17 Dec 2010 12:36:21 -0500 From: "Weems, Joyce" Subject: [Histonet] RE: Cassette/slide labelers - again To: Nancy Schmitt , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324C6@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We have GE Ultra - formerly Triple G. Cassettes are printed on our Sakura printer at accessioning (number is attached to the specimen) and more printed at grossing if needed. We use multiple colors of cassettes - the color is tied to the specimen of can be changed for decal. Slides are printed on our Sakura slide printer in batch by case type. Charged slides on in one hopper and printing is directed there for the necessary printing through the soft ware. It was painful getting the interface done, but once it was, it has been a good thing. Good luck, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, December 17, 2010 12:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette/slide labelers - again Sorry to boot this dog around a bit more, BUT - We have Cerner CoPath plus and Dragon speak and are looking at Thermo and General Data. Do they interface smoothly? Do your cassettes get printed at accession or grossing? Are you using multiple colors of cassettes? What are you doing for slides that require levels or a mix of non-charged and charged slides? Thank you for any and all input! Nancy Schmitt, MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 12 Date: Fri, 17 Dec 2010 12:39:28 -0500 From: "Rathborne, Toni" Subject: RE: [Histonet] RE: Cassette/slide labelers - again To: "Weems, Joyce" , "Nancy Schmitt" , Message-ID: Content-Type: text/plain; charset="utf-8" Do you find that the number of errors has decreased since you have implemented this? Our pathologists are hesitant because they worry too many pre-printed cassettes could get mixed up. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 12:36 PM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette/slide labelers - again We have GE Ultra - formerly Triple G. Cassettes are printed on our Sakura printer at accessioning (number is attached to the specimen) and more printed at grossing if needed. We use multiple colors of cassettes - the color is tied to the specimen of can be changed for decal. Slides are printed on our Sakura slide printer in batch by case type. Charged slides on in one hopper and printing is directed there for the necessary printing through the soft ware. It was painful getting the interface done, but once it was, it has been a good thing. Good luck, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, December 17, 2010 12:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette/slide labelers - again Sorry to boot this dog around a bit more, BUT - We have Cerner CoPath plus and Dragon speak and are looking at Thermo and General Data. Do they interface smoothly? Do your cassettes get printed at accession or grossing? Are you using multiple colors of cassettes? What are you doing for slides that require levels or a mix of non-charged and charged slides? Thank you for any and all input! Nancy Schmitt, MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 13 Date: Fri, 17 Dec 2010 12:43:13 -0500 From: Bryan Watson Subject: [Histonet] Cap guidelines To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hey, I have a question. I was wondering if there are what the CAP regulations are for grossing, and if cutting bones for diagnosis falls into the same category as grossing other tissues. In out lab we have a PA who grosses all of the specimens. However the HT's cut down bone sections for bones that have avascular necrosis, fracture, etc. . . as the pre surgical diagnosis. I am trying to find out if this practice would be ok in the eyes of CAP or if the PA should be doing that as well. Thank you, Bryan HT (ASCP) ------------------------------ Message: 14 Date: Fri, 17 Dec 2010 12:43:21 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] RE: Cassette/slide labelers - again To: "Rathborne, Toni" , Nancy Schmitt , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324CB@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I don't have documentation to say there's a decrease or increase, but we don't find it to be a problem at all. The cassettes are put on top of each container and seem to live there. We do have them bar-coded and in our ideal world, we would check, but our system doesn't work all the way through yet! -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Friday, December 17, 2010 12:39 To: Weems, Joyce; Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cassette/slide labelers - again Do you find that the number of errors has decreased since you have implemented this? Our pathologists are hesitant because they worry too many pre-printed cassettes could get mixed up. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 12:36 PM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette/slide labelers - again We have GE Ultra - formerly Triple G. Cassettes are printed on our Sakura printer at accessioning (number is attached to the specimen) and more printed at grossing if needed. We use multiple colors of cassettes - the color is tied to the specimen of can be changed for decal. Slides are printed on our Sakura slide printer in batch by case type. Charged slides on in one hopper and printing is directed there for the necessary printing through the soft ware. It was painful getting the interface done, but once it was, it has been a good thing. Good luck, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, December 17, 2010 12:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette/slide labelers - again Sorry to boot this dog around a bit more, BUT - We have Cerner CoPath plus and Dragon speak and are looking at Thermo and General Data. Do they interface smoothly? Do your cassettes get printed at accession or grossing? Are you using multiple colors of cassettes? What are you doing for slides that require levels or a mix of non-charged and charged slides? Thank you for any and all input! Nancy Schmitt, MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 15 Date: Fri, 17 Dec 2010 10:46:15 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] Physician Signatures To: "Weems, Joyce" , "WILLIAM DESALVO" , "histonet" Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C6ADC@EXCHANGECLUSTER.yumaregional.local > Content-Type: text/plain; charset="us-ascii" This is true about electronic ordering but there is a provision on what is acceptable for electronic order, just ordering in a system and spitting out requisitions does not comply, There needs to be password verification and review of this information by the clinician before it is ordered and sent to the laboratory performing the service when the requisition is created. They go on to say that orders and requisitions are different aspects and should not be confused. IF the orders are just received through an electronic interface then there is a difference. But if the specimen is received using any paper form then this needs to be signed by the clinician either electronically or manually. We are looking at this really closely, with using paper requisitions, even orders from the OR for frozens, molecular studies, interoperative work, all needs to be signed by the surgeon. Can you imagine the impact that this is going to cause. Our current solutions is to have maybe standing orders in place and move and look at process within the Main OR's, outpatient OR and clients. Once again a huge problem,, but Bill is right this will lead to more and more paperless enviroments. Jesus Ellin Yuma Regional Medical Center ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ ------------------------------ Message: 16 Date: Fri, 17 Dec 2010 12:55:15 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] Physician Signatures To: Jesus Ellin , WILLIAM DESALVO , histonet Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B3324D1@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I had that concern about our electronic orders, because it certainly is just spitting out a paper. We must do something! How can we make CMS hear us? ASCP just send an email that a hospital is OR says it is unjust. j -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, December 17, 2010 12:46 To: Weems, Joyce; WILLIAM DESALVO; histonet Subject: RE: [Histonet] Physician Signatures This is true about electronic ordering but there is a provision on what is acceptable for electronic order, just ordering in a system and spitting out requisitions does not comply, There needs to be password verification and review of this information by the clinician before it is ordered and sent to the laboratory performing the service when the requisition is created. They go on to say that orders and requisitions are different aspects and should not be confused. IF the orders are just received through an electronic interface then there is a difference. But if the specimen is received using any paper form then this needs to be signed by the clinician either electronically or manually. We are looking at this really closely, with using paper requisitions, even orders from the OR for frozens, molecular studies, interoperative work, all needs to be signed by the surgeon. Can you imagine the impact that this is going to cause. Our current solutions is to have maybe standing orders in place and move and look at process within the Main OR's, outpatient OR and clients. Once again a huge problem,, but Bill is right this will lead to more and more paperless enviroments. Jesus Ellin Yuma Regional Medical Center ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 85, Issue 13 **************************************** From AnthonyH <@t> chw.edu.au Mon Dec 20 15:30:15 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Dec 20 15:30:27 2010 Subject: [Histonet] need ur help In-Reply-To: <517559.68856.qm@web45209.mail.sp1.yahoo.com> References: <517559.68856.qm@web45209.mail.sp1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A7157026642@xmdb02.nch.kids> Check the fixative the biopsies are going into. Is it 10% neutral buffered formalin? Are they being fixed for long enough? Remember these are usually small, thin biopsies so they do not need extended processing so use the time to extend the fixation. You could try placing the biopsies in fixative in a warm oven (37-45oC) before loading onto the processor. Are they being allowed to dry before being placed in fixative? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wassan alkadhumi Sent: Tuesday, 21 December 2010 4:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need ur help Hi all Hope u r?all doing well, am an?Iraqi histotech working in both histopathology?and IHC department?in Iraq.?One part of what i do is IHC (manual)?i have no problem with,?the other part is what?i have problems with, Renals and Livers,?we do regular?H?&E stain?, PAS and Masson Tirchrome?and all of them?were text book quality until ten days ago, the problem is?the Stains even the H&E are not Fine, its smugish. And?the most important stain? for the histopathologist is the Trichrome?which it should be fine and delacate and its not.?I checked the procedures and stain preparations?and i doing it the correctly ,?I made new solutions and?did?prolonged deparaffinization 10 min each step (3 xylenes,??2?100%ETOH,??one 90%ETOH,?one?70%ETOH) and there was?no difference.?I should?mention that?those problems happened with only Renal and Liver biopsies the H&E and PAS on other types of tissues are working just great.?the?one thing that?changed before 10 days ago?is the wether it became really cold and our lab temp. is not controlled.??Am going crazy please help me Thank u a lot Wassan Histotech. Shorsh hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From gupathlab <@t> gmail.com Mon Dec 20 15:47:31 2010 From: gupathlab <@t> gmail.com (lab operations) Date: Mon Dec 20 15:47:33 2010 Subject: [Histonet] Columbus, Ohio employment Histotechs and cytotechs Message-ID: Looking for experienced histology technicians and cytotechnologists in Columbus, Ohio for new GU pathology laboratory. Excellent compensation with great benefits. Experience with IHC, FISH, and GU pathology a plus. Send resume or CV to careers@aksm.com From pruegg <@t> ihctech.net Mon Dec 20 16:09:26 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Dec 20 16:10:21 2010 Subject: SPAM-LOW: RE: [Histonet] need ur help In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157026642@xmdb02.nch.kids> References: <517559.68856.qm@web45209.mail.sp1.yahoo.com> <6D6BD1DE8A5571489398B392A38A7157026642@xmdb02.nch.kids> Message-ID: <2A9061E0156E4AB39302E2F5931CEE19@prueggihctechlt> Yea I would say make sure the samples are getting fixed long enough, fixation is also temp dependent so if it is colder now you might have to fix longer, try and fix your tissue, even biopsies for 24 hours if you can in 10% Neutral phosphate buffered formalin. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, December 20, 2010 2:30 PM To: 'wassan alkadhumi'; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] need ur help Check the fixative the biopsies are going into. Is it 10% neutral buffered formalin? Are they being fixed for long enough? Remember these are usually small, thin biopsies so they do not need extended processing so use the time to extend the fixation. You could try placing the biopsies in fixative in a warm oven (37-45oC) before loading onto the processor. Are they being allowed to dry before being placed in fixative? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wassan alkadhumi Sent: Tuesday, 21 December 2010 4:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need ur help Hi all Hope u r?all doing well, am an?Iraqi histotech working in both histopathology?and IHC department?in Iraq.?One part of what i do is IHC (manual)?i have no problem with,?the other part is what?i have problems with, Renals and Livers,?we do regular?H?&E stain?, PAS and Masson Tirchrome?and all of them?were text book quality until ten days ago, the problem is?the Stains even the H&E are not Fine, its smugish. And?the most important stain? for the histopathologist is the Trichrome?which it should be fine and delacate and its not.?I checked the procedures and stain preparations?and i doing it the correctly ,?I made new solutions and?did?prolonged deparaffinization 10 min each step (3 xylenes,??2?100%ETOH,??one 90%ETOH,?one?70%ETOH) and there was?no difference.?I should?mention that?those problems happened with only Renal and Liver biopsies the H&E and PAS on other types of tissues are working just great.?the?one thing that?changed before 10 days ago?is the wether it became really cold and our lab temp. is not controlled.??Am going crazy please help me Thank u a lot Wassan Histotech. Shorsh hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **************************************************************************** ***** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Dec 20 16:14:32 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Dec 20 16:14:47 2010 Subject: [Histonet] need ur help In-Reply-To: References: <6D6BD1DE8A5571489398B392A38A7157026642@xmdb02.nch.kids> Message-ID: <6D6BD1DE8A5571489398B392A38A71570278B5@xmdb02.nch.kids> Kim, Did you send this to Histonet & Wassan? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Kim.Donadio@bhcpns.org [mailto:Kim.Donadio@bhcpns.org] Sent: Tuesday, 21 December 2010 9:00 AM To: Tony Henwood Subject: RE: [Histonet] need ur help Are the tiny biopsies freezing after you put them in paraffin? I have noticed that when you use freeze spray( in your case, maybe the coldness?) and the paraffin cracks it also cracks the tissue and you can see it microscopically. Just a thought. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Tony Henwood > Sent by: histonet-bounces@lists.utsouthwestern.edu 12/20/2010 03:30 PM To "'wassan alkadhumi'" >, "histonet@lists.utsouthwestern.edu" > cc Subject RE: [Histonet] need ur help Check the fixative the biopsies are going into. Is it 10% neutral buffered formalin? Are they being fixed for long enough? Remember these are usually small, thin biopsies so they do not need extended processing so use the time to extend the fixation. You could try placing the biopsies in fixative in a warm oven (37-45oC) before loading onto the processor. Are they being allowed to dry before being placed in fixative? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wassan alkadhumi Sent: Tuesday, 21 December 2010 4:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need ur help Hi all Hope u r all doing well, am an Iraqi histotech working in both histopathology and IHC department in Iraq. One part of what i do is IHC (manual) i have no problem with, the other part is what i have problems with, Renals and Livers, we do regular H &E stain , PAS and Masson Tirchrome and all of them were text book quality until ten days ago, the problem is the Stains even the H&E are not Fine, its smugish. And the most important stain for the histopathologist is the Trichrome which it should be fine and delacate and its not. I checked the procedures and stain preparations and i doing it the correctly , I made new solutions and did prolonged deparaffinization 10 min each step (3 xylenes, 2 100%ETOH, one 90%ETOH, one 70%ETOH) and there was no difference. I should mention that those problems happened with only Renal and Liver biopsies the H&E and PAS on other types of tissues are working just great. the one thing that changed before 10 days ago is the wether it became really cold and our lab temp. is not controlled. Am going crazy please help me Thank u a lot Wassan Histotech. Shorsh hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From Rcartun <@t> harthosp.org Mon Dec 20 16:49:17 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Dec 20 16:49:25 2010 Subject: [Histonet] OR simulation laboratory question Message-ID: <4D0F971C.7400.0077.1@harthosp.org> One of our Vice Presidents contacted me recently about the use of porcine tissue (colon, gallbladder, etc.) to train house staff and surgeons on how to use surgical instruments in our new OR simulation laboratory. Other than the obvious requirement of biohazard disposal, are there any other guidelines or regulations that need to be followed? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From amosbrooks <@t> gmail.com Mon Dec 20 18:06:59 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Dec 20 18:07:11 2010 Subject: [Histonet] comparisons between.... In-Reply-To: <4d0f99c6.12ac960a.2038.ffffddaeSMTPIN_ADDED@mx.google.com> References: <4d0f99c6.12ac960a.2038.ffffddaeSMTPIN_ADDED@mx.google.com> Message-ID: <201012201906.59879.amosbrooks@gmail.com> Hi, I always say that "No one company can do it all". Not all antibodies are made by all companies. Not all antibodies made by different companies are the same. There is a lot of re-selling and re-packaging of antibodies. So some antibodies that are indeed the same are often marked up considerably. It would be easier to compare specific antibodies from different companies. If you start with a few antibodies then getting suggestions from various user groups like this one as to which antibody folks use and under what conditions. Short of that you might need to compare them by trying them yourself. Some companies offer samples or trial sizes. Ultimately, if done right, you would end up with antibodies purchased from at least three companies. So to start the volley: what is the most common antibody you run? Amos On Monday 20 December 2010 01:00:38 pm histonet- request@lists.utsouthwestern.edu wrote: > Message: 4 > Date: Mon, 20 Dec 2010 19:49:25 +0500 (PKT) > From: tahseen@brain.net.pk > Subject: [Histonet] comparisons between Dako,Lieca,seroTec and > ????????CellMarque > To: histonet@lists.utsouthwestern.edu > Message-ID: <28745.203.135.35.66.1292856565.squirrel@brain.net.pk> > Content-Type: text/plain;charset=iso-8859-1 > > Dear All, > We are using Dako antibodies and EnVision kit for IHC on paraffin sections > from 15 years there are no problem with us. Now our MMD required > comparisons between other companies like Lieca,Cell Marque and SeroTec. > Would any one like to advise me? > Muhammad Tahseen > Shaukat Khanum Memorial Cancer Hospital & RC > Lahore Pakistan. From gu.lang <@t> gmx.at Tue Dec 21 04:09:53 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Dec 21 04:12:40 2010 Subject: AW: [Histonet] comparisons between Dako,Lieca,seroTec and CellMarque In-Reply-To: <28745.203.135.35.66.1292856565.squirrel@brain.net.pk> References: <28745.203.135.35.66.1292856565.squirrel@brain.net.pk> Message-ID: <22C71CBAA404466096B1F9C4163D292A@dielangs.at> I think, if you want an exact answer it will lead into titration of all available antibody you have in your panel. Experiences of other labs may help, but in the "fine-tuning" everyone has to find his own protocol. That's very much afford. In my opinion it would be better to look first at the overall prices of a certain company and range the interesting companies for your personal purpose (shipping-time, technical support). It makes sense to compare antibodies in use with insufficient results to other vendors. Or if you look for new antibodies. But a well working test..? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von tahseen@brain.net.pk Gesendet: Montag, 20. Dezember 2010 15:49 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] comparisons between Dako,Lieca,seroTec and CellMarque Dear All, We are using Dako antibodies and EnVision kit for IHC on paraffin sections from 15 years there are no problem with us. Now our MMD required comparisons between other companies like Lieca,Cell Marque and SeroTec. Would any one like to advise me? Muhammad Tahseen Shaukat Khanum Memorial Cancer Hospital & RC Lahore Pakistan. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Dec 21 04:14:10 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Dec 21 04:14:24 2010 Subject: AW: [Histonet] need ur help In-Reply-To: <517559.68856.qm@web45209.mail.sp1.yahoo.com> References: <517559.68856.qm@web45209.mail.sp1.yahoo.com> Message-ID: <72E72E1EFA47486896DC5E9F545CD3E4@dielangs.at> Is there a new procedure while biopsies are aquired? New doctor, new assistant? Somebody who lets dry out the biopsies? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von wassan alkadhumi Gesendet: Montag, 20. Dezember 2010 18:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] need ur help Hi all Hope u r?all doing well, am an?Iraqi histotech working in both histopathology?and IHC department?in Iraq.?One part of what i do is IHC (manual)?i have no problem with,?the other part is what?i have problems with, Renals and Livers,?we do regular?H?&E stain?, PAS and Masson Tirchrome?and all of them?were text book quality until ten days ago, the problem is?the Stains even the H&E are not Fine, its smugish. And?the most important stain? for the histopathologist is the Trichrome?which it should be fine and delacate and its not.?I checked the procedures and stain preparations?and i doing it the correctly ,?I made new solutions and?did?prolonged deparaffinization 10 min each step (3 xylenes,??2?100%ETOH,??one 90%ETOH,?one?70%ETOH) and there was?no difference.?I should?mention that?those problems happened with only Renal and Liver biopsies the H&E and PAS on other types of tissues are working just great.?the?one thing that?changed before 10 days ago?is the wether it became really cold and our lab temp. is not controlled.??Am going crazy please help me Thank u a lot Wassan Histotech. Shorsh hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BBranton <@t> sarapath.com Tue Dec 21 07:36:07 2010 From: BBranton <@t> sarapath.com (Brian Branton) Date: Tue Dec 21 07:35:49 2010 Subject: [Histonet] I have two items for sale on eBay, and some other equipment Message-ID: Hello Histonetters, I have two items for sale on eBay if anyone is interested. 8,500 P5049-15 - 1 to 200uL Nonfiltered Pipetter Tips http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=200556833206 Ventana Medical Systems - Ebar SLS Printer http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=200555458833 I also have some equipment for sale on our web site, and if you mention that you saw this notice on the HistoNet. I will knock $500.00 off either the Dako Autostainer or Ventana NEXus, and $100.00 off the Epson printer. Sorry, I don't have any Sakura Storage Cabinets until sometime in January. http://www.sarapath.com/equipment/ Thanks for looking, and Happy Holidays Brian Branton Purchasing Agent SaraPath Diagnostics Sarasota Pathology Sarasota Professional Enterprises II (941) 362-8963 (941) 362-8964 FAX From histology <@t> medsurgpath.com Tue Dec 21 09:38:47 2010 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Tue Dec 21 09:38:50 2010 Subject: [Histonet] Manual for Cryocut 1800 Message-ID: <7936848d90a78fc4f44823d3c7aaa3f8.squirrel@webmail.integra.net> Hi everyone, I was wondering if anyone has a copy of a manual for a Reichert-Jung/Leica Cryocut 1800 Cryostat with a Reichert-Jung 2020 Rotary Microtome they would be willing to share. Please contact me if you can help me out, thank you! Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 From Kim.Donadio <@t> bhcpns.org Tue Dec 21 10:09:52 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Dec 21 10:10:07 2010 Subject: [Histonet] need ur help In-Reply-To: <6D6BD1DE8A5571489398B392A38A71570278B5@xmdb02.nch.kids> Message-ID: Thought I did. Thanks for making sure. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Tony Henwood 12/20/2010 04:14 PM To "'Kim.Donadio@bhcpns.org'" cc "'w_alkadhumi@yahoo.com'" , "histonet@lists.utsouthwestern.edu" Subject RE: [Histonet] need ur help Kim, Did you send this to Histonet & Wassan? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Kim.Donadio@bhcpns.org [mailto:Kim.Donadio@bhcpns.org] Sent: Tuesday, 21 December 2010 9:00 AM To: Tony Henwood Subject: RE: [Histonet] need ur help Are the tiny biopsies freezing after you put them in paraffin? I have noticed that when you use freeze spray( in your case, maybe the coldness?) and the paraffin cracks it also cracks the tissue and you can see it microscopically. Just a thought. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Tony Henwood Sent by: histonet-bounces@lists.utsouthwestern.edu 12/20/2010 03:30 PM To "'wassan alkadhumi'" , " histonet@lists.utsouthwestern.edu" cc Subject RE: [Histonet] need ur help Check the fixative the biopsies are going into. Is it 10% neutral buffered formalin? Are they being fixed for long enough? Remember these are usually small, thin biopsies so they do not need extended processing so use the time to extend the fixation. You could try placing the biopsies in fixative in a warm oven (37-45oC) before loading onto the processor. Are they being allowed to dry before being placed in fixative? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wassan alkadhumi Sent: Tuesday, 21 December 2010 4:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need ur help Hi all Hope u r all doing well, am an Iraqi histotech working in both histopathology and IHC department in Iraq. One part of what i do is IHC (manual) i have no problem with, the other part is what i have problems with, Renals and Livers, we do regular H &E stain , PAS and Masson Tirchrome and all of them were text book quality until ten days ago, the problem is the Stains even the H&E are not Fine, its smugish. And the most important stain for the histopathologist is the Trichrome which it should be fine and delacate and its not. I checked the procedures and stain preparations and i doing it the correctly , I made new solutions and did prolonged deparaffinization 10 min each step (3 xylenes, 2 100%ETOH, one 90%ETOH, one 70%ETOH) and there was no difference. I should mention that those problems happened with only Renal and Liver biopsies the H&E and PAS on other types of tissues are working just great. the one thing that changed before 10 days ago is the wether it became really cold and our lab temp. is not controlled. Am going crazy please help me Thank u a lot Wassan Histotech. Shorsh hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From alisha <@t> ka-recruiting.com Tue Dec 21 10:33:57 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Dec 21 10:33:56 2010 Subject: [Histonet] High Paying Job Opportunity for Pathologist Assistants or Histotechnologists Message-ID: <503449091.1292949237488.JavaMail.cfservice@SL4APP4> Hi Histonet Members, I am a healthcare recruiter, specifically focused on the laboratory/pathology space. I have openings all across the country and would be interested in speaking with anyone interested in searching for a new job. I am currently working on an amazing opportunity with a cancer diagnostics company. This company is looking to hire on Technical Support Specialists in several areas across the country. They currently are looking for people located anywhere West of the Mississippi River or in Atlanta, GA or in Nashville, TN or in New York City or in Philadelphia. The TSS would be responsible for training pathologists, surgeons, radiologists, and pathologist assistant's to ensure adequate sampling on breast cancer and colon cancer tissues. They are looking for someone with a Bachelor's Degree and 5+ years experience in tissue grossing/sampling, histology, or in a pathology or surgical capacity. The ideal candidate with be PA(ASCP) or HTL(ASCP) certified. This position requires extensive travel, paid for by the company. The compensation package is fantastic with a high base salary and up to a 20% bonus. If interested in learning more details, please email me at alisha@ka-recruiting.com. If you are not interested but know of someone you could recommend for this position, please pass on their contact information to me. We have a referral bonus for anyone you refer to me that I place into a new job. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From histology <@t> medsurgpath.com Tue Dec 21 12:56:01 2010 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Tue Dec 21 12:56:05 2010 Subject: [Histonet] Manual for Cryocut 1800 In-Reply-To: <7936848d90a78fc4f44823d3c7aaa3f8.squirrel@webmail.integra.net> References: <7936848d90a78fc4f44823d3c7aaa3f8.squirrel@webmail.integra.net> Message-ID: <8daa5c6014df206512ccf16ebcaae26c.squirrel@webmail.integra.net> Thank you for your quick responses! I received a copy right away and really appreciate the effort. Merry Christmas, Histonet! Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > Hi everyone, > I was wondering if anyone has a copy of a manual for a > Reichert-Jung/Leica Cryocut 1800 Cryostat with a Reichert-Jung 2020 Rotary > Microtome they would be willing to share. > Please contact me if you can help me out, thank you! > > Katelin Lester, HTL(ASCP) > MedSurg Pathology Associates, Inc. > (503)443-2157 > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From settembr <@t> umdnj.edu Tue Dec 21 14:00:00 2010 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Tue Dec 21 14:00:16 2010 Subject: [Histonet] Edge affect on TMA? In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE287@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870E0FE287@dhmail.dhorg.org> Message-ID: Does anyone experience edge affect on TMA? Dana Settembre From Erik.Dokken <@t> onassignment.com Tue Dec 21 15:07:36 2010 From: Erik.Dokken <@t> onassignment.com (Erik Dokken) Date: Tue Dec 21 15:07:44 2010 Subject: [Histonet] Contract Position in Seattle, WA In-Reply-To: <201012211807.oBLI4A69012000@smtp12.onasgn.net> References: <201012211807.oBLI4A69012000@smtp12.onasgn.net> Message-ID: On Assignment Healthcare has an immediate opportunity for a qualified Histo Tech in the Seattle area. This is a full time 4 month contract assignment with the opportunity to extend. Our client is a large Dermatology Practice in the Seattle Area. Duties and Responsibilities include: - Provide quality tissue preparations from grossing, processing, embedding, cutting and staining tissue specimens from all patient populations according to current department protocols. - Perform, document and review quality control to assure the accuracy and staining quality of the slides. - Effectively operate, maintain and troubleshoot all equipment related to tissue preparations and storage. - Recognize, correct, and report problems that would cause inaccurate or misleading results. - Use appropriate handling of hazardous chemicals used for tissue preparation. Requirements Include: - Associates Degree with ASCP or equivalent registry. - Graduate of an accredited Histology Program. - EPIC knowledge preferred. - 1 year of recent paid Histo Tech experience. If you or someone you know is interested in this position contact us today! Erik Dokken, On Assignment Healthcare, erik.dokken@onassignment.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, December 21, 2010 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 85, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. High Paying Job Opportunity for Pathologist Assistants or Histotechnologists (Alisha Dynan) ---------------------------------------------------------------------- Message: 1 Date: 21 Dec 2010 11:33:57 -0500 From: Alisha Dynan Subject: [Histonet] High Paying Job Opportunity for Pathologist Assistants or Histotechnologists To: histonet@lists.utsouthwestern.edu Message-ID: <503449091.1292949237488.JavaMail.cfservice@SL4APP4> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am a healthcare recruiter, specifically focused on the laboratory/pathology space. I have openings all across the country and would be interested in speaking with anyone interested in searching for a new job. I am currently working on an amazing opportunity with a cancer diagnostics company. This company is looking to hire on Technical Support Specialists in several areas across the country. They currently are looking for people located anywhere West of the Mississippi River or in Atlanta, GA or in Nashville, TN or in New York City or in Philadelphia. The TSS would be responsible for training pathologists, surgeons, radiologists, and pathologist assistant's to ensure adequate sampling on breast cancer and colon cancer tissues. They are looking for someone with a Bachelor's Degree and 5+ years experience in tissue grossing/sampling, histology, or in a pathology or surgical capacity. The ideal candidate with be PA(ASCP) or HTL(ASCP) certified. This position requires extensive travel, paid for by the company. The compensation package is fantastic with a high base salary and up to a 20% bonus. If interested in learning more details, please email me at alisha@ka-recruiting.com. If you are not interested but know of someone you could recommend for this position, please pass on their contact information to me. We have a referral bonus for anyone you refer to me that I place into a new job. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 85, Issue 18 **************************************** From ynwang <@t> u.washington.edu Tue Dec 21 15:07:35 2010 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Tue Dec 21 15:07:58 2010 Subject: [Histonet] paraformaldehyde vapour Message-ID: Hello, In a protocol to stain mucin in oesophageal tissue, it calls for "post-fix in paraformaldehyde vapour at 37 deg C". The protocol is a modified Alcian blue/PAS staining technique. Can someone share insight into how to fix in paraformaldehyde vapour? That is, how much paraformaldehye powder is needed per container area/# of slides. Do I just place powder at the bottom of a dish with the slides and place in at 37 deg C? Thank you for your help Yak-Nam Wang University of Washington APL Seattle, WA 98105 From hfedor <@t> jhmi.edu Tue Dec 21 15:13:31 2010 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Tue Dec 21 15:13:36 2010 Subject: [Histonet] RE: Edge affect on TMA? In-Reply-To: References: <38A56C4F4630D348A50B3720409270870E0FE287@dhmail.dhorg.org> Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D317858318A7@JHEMTEXVS3.win.ad.jhu.edu> Hello, We do not usually see that. If we do its very rare because I can't think of any examples off hand. The usual problems are the spots came off or shifted/smushed, not an edge effect. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Tuesday, December 21, 2010 3:00 PM To: 'Hutton, Allison'; histonet@lists.utsouthwestern.edu Subject: [Histonet] Edge affect on TMA? Does anyone experience edge affect on TMA? Dana Settembre _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laura.Miller <@t> leica-microsystems.com Tue Dec 21 15:28:57 2010 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Tue Dec 21 15:29:02 2010 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 12/21/2010 and will not return until 12/28/2010. I will be out of the office until 12-28. I will respond to your email whem I return. Have a safe and merry holiday season!!! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Nacaela.Johnson <@t> USONCOLOGY.COM Tue Dec 21 15:44:16 2010 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Tue Dec 21 15:44:20 2010 Subject: [Histonet] ISH Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D454@txhous1eb012.uson.usoncology.int> Would anyone care to share their ISH protocols? Especially ISH double staining with DAB and Refine Red. I use the Leica Bond Max. I am not having luck optimizing this protocol. Happy Holidays!! Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From irena.kirbis <@t> hotmail.com Wed Dec 22 00:53:40 2010 From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS) Date: Wed Dec 22 00:53:46 2010 Subject: [Histonet] RE: Edge affect on TMA? In-Reply-To: <3201CF51728F6048A24FA3AFFFEEF1D317858318A7@JHEMTEXVS3.win.ad.jhu.edu> References: <38A56C4F4630D348A50B3720409270870E0FE287@dhmail.dhorg.org>, , <3201CF51728F6048A24FA3AFFFEEF1D317858318A7@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: how to prevent spots coming off during sectioning TM's? we do sometimes facing problems sectioning TM's, they're just falling apart? thanks for any hints Irena > From: hfedor@jhmi.edu > To: settembr@umdnj.edu; AHutton@dh.org; histonet@lists.utsouthwestern.edu > Date: Tue, 21 Dec 2010 16:13:31 -0500 > CC: > Subject: [Histonet] RE: Edge affect on TMA? > > Hello, We do not usually see that. If we do its very rare because I can't think of any examples off hand. The usual problems are the spots came off or shifted/smushed, not an edge effect. > > Helen > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana > Sent: Tuesday, December 21, 2010 3:00 PM > To: 'Hutton, Allison'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] Edge affect on TMA? > > Does anyone experience edge affect on TMA? > Dana Settembre > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Wed Dec 22 06:45:40 2010 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Wed Dec 22 06:46:06 2010 Subject: [Histonet] ISH edge effect on TMA In-Reply-To: References: <38A56C4F4630D348A50B3720409270870E0FE287@dhmail.dhorg.org> <3201CF51728F6048A24FA3AFFFEEF1D317858318A7@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: Has anyone experience edge affect on TMA when doing ISH? Dana Settembre From hfedor <@t> jhmi.edu Wed Dec 22 07:52:40 2010 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Wed Dec 22 07:52:46 2010 Subject: [Histonet] RE: Edge affect on TMA? In-Reply-To: References: <38A56C4F4630D348A50B3720409270870E0FE287@dhmail.dhorg.org> <3201CF51728F6048A24FA3AFFFEEF1D317858318A7@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D31785831A3F@JHEMTEXVS3.win.ad.jhu.edu> Here is a hint to keep your TMA from falling apart. After construction put the block face down on a glass slide a put in a 37degree C oven for 15-30 minutes (the paraffin should not be allowed to melt). Then take the block/slide out and gently compress with even pressure. (uneven pressure will cause the block to become misshapen). Cool the block at room temperature. You can repeat this process several times to help the cores adhere to the recipient block. The larger the core size, the more severe this problem is. One of the reasons that we choose not to use the 2.0 mm core is that this problem is more severe with this size core. Hope that will help you. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 410.614.1660 From: IRENA SREBOTNIK KIRBIS [mailto:irena.kirbis@hotmail.com] Sent: Wednesday, December 22, 2010 1:54 AM To: Helen Fedor; settembr@umdnj.edu; ahutton@dh.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Edge affect on TMA? how to prevent spots coming off during sectioning TM's? we do sometimes facing problems sectioning TM's, they're just falling apart? thanks for any hints Irena > From: hfedor@jhmi.edu > To: settembr@umdnj.edu; AHutton@dh.org; histonet@lists.utsouthwestern.edu > Date: Tue, 21 Dec 2010 16:13:31 -0500 > CC: > Subject: [Histonet] RE: Edge affect on TMA? > > Hello, We do not usually see that. If we do its very rare because I can't think of any examples off hand. The usual problems are the spots came off or shifted/smushed, not an edge effect. > > Helen > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana > Sent: Tuesday, December 21, 2010 3:00 PM > To: 'Hutton, Allison'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] Edge affect on TMA? > > Does anyone experience edge affect on TMA? > Dana Settembre > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Dec 22 08:49:29 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Dec 22 08:50:11 2010 Subject: [Histonet] Happy Holidays from Pam Barker and RELIA Message-ID: <7E0F3C6C5FFC47188EA2B9C4B995766C@ownerf1abaad51> Happy Holidays Histonetters!! I hope you are enjoying a wonderful holiday season. I need your help. I need a really unique and yummy cookie recipe for a cookie swap. What would you make? Do you have a recipe to share? If you have a minute Iwould like to tell you about the jobs that I am working on. All my clients are willing to move now or wait until after the holidays to accommodate you. The labs and hospitals that I work with offer excellent compensation packages including great pay and benefits and relocation assistance. Here is a list of my current openings: MANAGEMENT: NH- Histology Supervisor Manchester, NH CA - Histology Lab Manager ? Central Valley of CA CO - Histology Supervisor ? Colorado Springs, CO LA - Histology Supervisor ? Baton Rouge, LA HISTOTECHNICIANS/HISTOTECHNOLOGISTS TX ? Austin ? Great lab and crew to work with. TN ? Nashville Histotech -night shifts avail. TN ? Chattanooga Grossing Histotech CA ? Northern CA-Inside Sales/Technical Support ? TEM exper required. NY- Orange/Rockland County Brand New Lab NYS license NY- Orange/Rockland County ? Immunohistochemistry Specialist NY ? Rochester Dermpath Histotech NYS license required FL ? Orlando FL Technologist license required. If you or anyone you know might be interested in hearing about any of these opportunities or are looking for a new position in another area please feel free to contact me or pass along my contact information to them. Now back to the cookies Whatcha Got? Thanks and again Happy Holidays to you and yours! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From wdesalvo.cac <@t> hotmail.com Wed Dec 22 09:44:34 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Dec 22 09:44:38 2010 Subject: [Histonet] CMS Postpones Deadline for Physician Signatures In-Reply-To: References: , , , Message-ID: I have just received notice that the CMS requirement for Physician Signature Deadline of Jan 1, 2011 has been postponed. http://www.cms.gov/ClinicalLabFeeSched/ CMS has decided that in the first quarter of 2011 they will be developing educational and outreach materials to help educate all of us that are affected by the policy change. Looks like we have a little more time to figure this new process out. William DeSalvo, B.S., HTL(ASCP) > From: wdesalvo.cac@hotmail.com > To: trathborne@somerset-healthcare.com; jellin@yumaregional.org; jweems@sjha.org; histonet@lists.utsouthwestern.edu > Date: Fri, 17 Dec 2010 12:38:12 -0700 > Subject: RE: [Histonet] Physician Signatures > CC: > > > They sign into the case prior to surgery, they have Physician Assistant (PA) assigned to the case, a nurse is assigned to the case and they then enter additional information into the system after they complete surgery. It meets requirements. In Pathology, many of your Frozen Section specimens arrive in the lab on verbal orders or nursing applying a signature and we have processed for years. I believe the key point that you have to wrap your head around is that the electronic orders by the surgeon are complete before there is any delivery of results, the signed and preliminary or final report by the pathologist and that meets regulatory requirements. I have more concern on the clinical side. Orders can be entered, tests performed and results delivered more easily than in Pathology. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > Subject: RE: [Histonet] Physician Signatures > > Date: Fri, 17 Dec 2010 14:06:04 -0500 > > From: trathborne@somerset-healthcare.com > > To: wdesalvo.cac@hotmail.com; jellin@yumaregional.org; jweems@sjha.org; histonet@lists.utsouthwestern.edu > > > > So how do the surgeons electronically order a test? Do they order it before the procedure? What if they discover something and need to add an additional specimen? Our orders are electronically entered in the OR during the procedure. The surgeon is scrubbed and performing the procedure while the circulating nurse places the order. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of WILLIAM > > DESALVO > > Sent: Friday, December 17, 2010 1:56 PM > > To: Jesus Ellin; jweems@sjha.org; histonet > > Subject: RE: [Histonet] Physician Signatures > > > > > > > > I previously did not go into to deep detail about security and compliance, but in our system all computer portals are password protected, orders are signed with electronic signature of the ordering person and when a hard copy paper form is printed, the ordering physician's electronic signature is applied. It is the same type of process and signature that is used by the pathologist to electronically sign off all pathology reports. I absolutely agree that you must control access to ordering and maintain integrity in the system. As you work through your process to develop a more electronic system, you will have the opportunity to build in and require proper controls. With those controls implemented, you can then become confident that the electronic process can and will meet your regulatory requirements. > > > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > > > > > > > Subject: RE: [Histonet] Physician Signatures > > > Date: Fri, 17 Dec 2010 10:46:15 -0700 > > > From: JEllin@yumaregional.org > > > To: JWeems@sjha.org; wdesalvo.cac@hotmail.com; histonet@lists.utsouthwestern.edu > > > > > > This is true about electronic ordering but there is a provision on what > > > is acceptable for electronic order, just ordering in a system and > > > spitting out requisitions does not comply, There needs to be password > > > verification and review of this information by the clinician before it > > > is ordered and sent to the laboratory performing the service when the > > > requisition is created. They go on to say that orders and requisitions > > > are different aspects and should not be confused. IF the orders are just > > > received through an electronic interface then there is a difference. > > > But if the specimen is received using any paper form then this needs to > > > be signed by the clinician either electronically or manually. > > > > > > We are looking at this really closely, with using paper requisitions, > > > even orders from the OR for frozens, molecular studies, interoperative > > > work, all needs to be signed by the surgeon. Can you imagine the impact > > > that this is going to cause. > > > > > > Our current solutions is to have maybe standing orders in place and move > > > and look at process within the Main OR's, outpatient OR and clients. > > > Once again a huge problem,, but Bill is right this will lead to more and > > > more paperless enviroments. > > > > > > > > > Jesus Ellin > > > Yuma Regional Medical Center > > > > > > ______________________________________________________________________ > > > This message is confidential, intended only for the named > > > recipient(s) and may contain information that is privileged > > > or exempt from disclosure under applicable law. If you are > > > not the intended recipient(s), you are notified that the > > > dissemination, distribution, or copying of this message is > > > strictly prohibited. If you receive this message in error, > > > or are not the named recipient(s), please notify the sender > > > at either the e-mail, fax, address, or telephone number > > > listed above and delete this e-mail from your computer. > > > Thank You. > > > ______________________________________________________________________ > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > CONFIDENTIALITY NOTICE > > This message and any included attachments are from Somerset Medical Center > > and are intended only for the addressee. The information contained in this > > message is confidential and may contain privileged, confidential, > > proprietary and/or trade secret information entitled to protection and/or > > exemption from disclosure under applicable law. Unauthorized forwarding, > > printing, copying, distribution, or use of such information is strictly > > prohibited and may be unlawful. If you are not the addressee, please > > promptly delete this message and notify the sender of the delivery error > > by e-mail or you may call Somerset Medical Center's computer Help Desk > > at 908-685-2200, ext. 4050. > > > > Be sure to visit Somerset Medical Center's Web site - > > www.somersetmedicalcenter.com - for the most up-to-date news, > > event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Mazan <@t> tufts.edu Wed Dec 22 09:58:48 2010 From: Melissa.Mazan <@t> tufts.edu (Mazan, Melissa) Date: Wed Dec 22 10:01:03 2010 Subject: [Histonet] long term storage of tissue Message-ID: <5E743323406EA847A2B1B38A3A2FF5CC04928A@TFTMEXDAG01MB03.tufts.ad.tufts.edu> Hi all, I have lungs that have been inflated with formalin/agar for a stereology study - mice had also been treated wtih BRDU. Problem is - took the mice down today and now find out that I cannot get them into paraffin for at least a week. Lungs are in formalin now - should I change them to 70% ethanol for long term (7-10 day) storage? Thanks! Melissa Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor, Director of Equine Sports Medicine Department of Clinical Sciences Cummings School of Veterinary Medicine Tufts University 200 Westborough Road North Grafton, MA, 01536 tel: 508-839-5395 email: melissa.mazan@tufts.edu From JEllin <@t> yumaregional.org Wed Dec 22 10:09:32 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Dec 22 10:12:09 2010 Subject: [Histonet] Re: CMS Postpones Deadline for Physician Signatures In-Reply-To: References: , , , Message-ID: Yes we received the update. But there is still questions about difference from electronic ordering CPOE vs review and verify correct entry before proccessing by a clinician. Even though it states that this does not address electronic orders. We feel that this can be a problem for the future. Sent via DROID one Verizon Wireless -----Original message----- From: WILLIAM DESALVO To: trathborne@somerset-healthcare.com, Jesus Ellin , jweems@sjha.org, histonet Sent: Wed, Dec 22, 2010 15:44:34 GMT+00:00 Subject: CMS Postpones Deadline for Physician Signatures I have just received notice that the CMS requirement for Physician Signature Deadline of Jan 1, 2011 has been postponed. http://www.cms.gov/ClinicalLabFeeSched/ CMS has decided that in the first quarter of 2011 they will be developing educational and outreach materials to help educate all of us that are affected by the policy change. Looks like we have a little more time to figure this new process out. William DeSalvo, B.S., HTL(ASCP) > From: wdesalvo.cac@hotmail.com > To: trathborne@somerset-healthcare.com; jellin@yumaregional.org; jweems@sjha.org; histonet@lists.utsouthwestern.edu > Date: Fri, 17 Dec 2010 12:38:12 -0700 > Subject: RE: [Histonet] Physician Signatures > CC: > > > They sign into the case prior to surgery, they have Physician Assistant (PA) assigned to the case, a nurse is assigned to the case and they then enter additional information into the system after they complete surgery. It meets requirements. In Pathology, many of your Frozen Section specimens arrive in the lab on verbal orders or nursing applying a signature and we have processed for years. I believe the key point that you have to wrap your head around is that the electronic orders by the surgeon are complete before there is any delivery of results, the signed and preliminary or final report by the pathologist and that meets regulatory requirements. I have more concern on the clinical side. Orders can be entered, tests performed and results delivered more easily than in Pathology. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > Subject: RE: [Histonet] Physician Signatures > > Date: Fri, 17 Dec 2010 14:06:04 -0500 > > From: trathborne@somerset-healthcare.com > > To: wdesalvo.cac@hotmail.com; jellin@yumaregional.org; jweems@sjha.org; histonet@lists.utsouthwestern.edu > > > > So how do the surgeons electronically order a test? Do they order it before the procedure? What if they discover something and need to add an additional specimen? Our orders are electronically entered in the OR during the procedure. The surgeon is scrubbed and performing the procedure while the circulating nurse places the order. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of WILLIAM > > DESALVO > > Sent: Friday, December 17, 2010 1:56 PM > > To: Jesus Ellin; jweems@sjha.org; histonet > > Subject: RE: [Histonet] Physician Signatures > > > > > > > > I previously did not go into to deep detail about security and compliance, but in our system all computer portals are password protected, orders are signed with electronic signature of the ordering person and when a hard copy paper form is printed, the ordering physician's electronic signature is applied. It is the same type of process and signature that is used by the pathologist to electronically sign off all pathology reports. I absolutely agree that you must control access to ordering and maintain integrity in the system. As you work through your process to develop a more electronic system, you will have the opportunity to build in and require proper controls. With those controls implemented, you can then become confident that the electronic process can and will meet your regulatory requirements. > > > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > > > > > > > Subject: RE: [Histonet] Physician Signatures > > > Date: Fri, 17 Dec 2010 10:46:15 -0700 > > > From: JEllin@yumaregional.org > > > To: JWeems@sjha.org; wdesalvo.cac@hotmail.com; histonet@lists.utsouthwestern.edu > > > > > > This is true about electronic ordering but there is a provision on what > > > is acceptable for electronic order, just ordering in a system and > > > spitting out requisitions does not comply, There needs to be password > > > verification and review of this information by the clinician before it > > > is ordered and sent to the laboratory performing the service when the > > > requisition is created. They go on to say that orders and requisitions > > > are different aspects and should not be confused. IF the orders are just > > > received through an electronic interface then there is a difference. > > > But if the specimen is received using any paper form then this needs to > > > be signed by the clinician either electronically or manually. > > > > > > We are looking at this really closely, with using paper requisitions, > > > even orders from the OR for frozens, molecular studies, interoperative > > > work, all needs to be signed by the surgeon. Can you imagine the impact > > > that this is going to cause. > > > > > > Our current solutions is to have maybe standing orders in place and move > > > and look at process within the Main OR's, outpatient OR and clients. > > > Once again a huge problem,, but Bill is right this will lead to more and > > > more paperless enviroments. > > > > > > > > > Jesus Ellin > > > Yuma Regional Medical Center > > > > > > ______________________________________________________________________ > > > This message is confidential, intended only for the named > > > recipient(s) and may contain information that is privileged > > > or exempt from disclosure under applicable law. If you are > > > not the intended recipient(s), you are notified that the > > > dissemination, distribution, or copying of this message is > > > strictly prohibited. If you receive this message in error, > > > or are not the named recipient(s), please notify the sender > > > at either the e-mail, fax, address, or telephone number > > > listed above and delete this e-mail from your computer. > > > Thank You. > > > ______________________________________________________________________ > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > CONFIDENTIALITY NOTICE > > This message and any included attachments are from Somerset Medical Center > > and are intended only for the addressee. The information contained in this > > message is confidential and may contain privileged, confidential, > > proprietary and/or trade secret information entitled to protection and/or > > exemption from disclosure under applicable law. Unauthorized forwarding, > > printing, copying, distribution, or use of such information is strictly > > prohibited and may be unlawful. If you are not the addressee, please > > promptly delete this message and notify the sender of the delivery error > > by e-mail or you may call Somerset Medical Center's computer Help Desk > > at 908-685-2200, ext. 4050. > > > > Be sure to visit Somerset Medical Center's Web site - > > www.somersetmedicalcenter.com - for the most up-to-date news, > > event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From JWeems <@t> sjha.org Wed Dec 22 10:24:22 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Dec 22 10:24:28 2010 Subject: [Histonet] RE: CMS Postpones Deadline for Physician Signatures In-Reply-To: References: , , , Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332AB1@CHEXCMS10.one.ads.che.org> Thanks for sharing!! ________________________________ From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Wednesday, December 22, 2010 10:45 To: trathborne@somerset-healthcare.com; Jesus Ellin; Weems, Joyce; histonet Subject: CMS Postpones Deadline for Physician Signatures I have just received notice that the CMS requirement for Physician Signature Deadline of Jan 1, 2011 has been postponed. http://www.cms.gov/ClinicalLabFeeSched/ CMS has decided that in the first quarter of 2011 they will be developing educational and outreach materials to help educate all of us that are affected by the policy change. Looks like we have a little more time to figure this new process out. William DeSalvo, B.S., HTL(ASCP) > From: wdesalvo.cac@hotmail.com > To: trathborne@somerset-healthcare.com; jellin@yumaregional.org; jweems@sjha.org; histonet@lists.utsouthwestern.edu > Date: Fri, 17 Dec 2010 12:38:12 -0700 > Subject: RE: [Histonet] Physician Signatures > CC: > > > They sign into the case prior to surgery, they have Physician Assistant (PA) assigned to the case, a nurse is assigned to the case and they then enter additional information into the system after they complete surgery. It meets requirements. In Pathology, many of your Frozen Section specimens arrive in the lab on verbal orders or nursing applying a signature and we have processed for years. I believe the key point that you have to wrap your head around is that the electronic orders by the surgeon are complete before there is any delivery of results, the signed and preliminary or final report by the pathologist and that meets regulatory requirements. I have more concern on the clinical side. Orders can be entered, tests performed and results delivered more easily than in Pathology. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > Subject: RE: [Histonet] Physician Signatures > > Date: Fri, 17 Dec 2010 14:06:04 -0500 > > From: trathborne@somerset-healthcare.com > > To: wdesalvo.cac@hotmail.com; jellin@yumaregional.org; jweems@sjha.org; histonet@lists.utsouthwestern.edu > > > > So how do the surgeons electronically order a test? Do they order it before the procedure? What if they discover something and need to add an additional specimen? Our orders are electronically entered in the OR during the procedure. The surgeon is scrubbed and performing the procedure while the circulating nurse places the order. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of WILLIAM > > DESALVO > > Sent: Friday, December 17, 2010 1:56 PM > > To: Jesus Ellin; jweems@sjha.org; histonet > > Subject: RE: [Histonet] Physician Signatures > > > > > > > > I previously did not go into to deep detail about security and compliance, but in our system all computer portals are password protected, orders are signed with electronic signature of the ordering person and when a hard copy paper form is printed, the ordering physician's electronic signature is applied. It is the same type of process and signature that is used by the pathologist to electronically sign off all pathology reports. I absolutely agree that you must control access to ordering and maintain integrity in the system. As you work through your process to develop a more electronic system, you will have the opportunity to build in and require proper controls. With those controls implemented, you can then become confident that the electronic process can and will meet your regulatory requirements. > > > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > > > > > > > Subject: RE: [Histonet] Physician Signatures > > > Date: Fri, 17 Dec 2010 10:46:15 -0700 > > > From: JEllin@yumaregional.org > > > To: JWeems@sjha.org; wdesalvo.cac@hotmail.com; histonet@lists.utsouthwestern.edu > > > > > > This is true about electronic ordering but there is a provision on what > > > is acceptable for electronic order, just ordering in a system and > > > spitting out requisitions does not comply, There needs to be password > > > verification and review of this information by the clinician before it > > > is ordered and sent to the laboratory performing the service when the > > > requisition is created. They go on to say that orders and requisitions > > > are different aspects and should not be confused. IF the orders are just > > > received through an electronic interface then there is a difference. > > > But if the specimen is received using any paper form then this needs to > > > be signed by the clinician either electronically or manually. > > > > > > We are looking at this really closely, with using paper requisitions, > > > even orders from the OR for frozens, molecular studies, interoperative > > > work, all needs to be signed by the surgeon. Can you imagine the impact > > > that this is going to cause. > > > > > > Our current solutions is to have maybe standing orders in place and move > > > and look at process within the Main OR's, outpatient OR and clients. > > > Once again a huge problem,, but Bill is right this will lead to more and > > > more paperless enviroments. > > > > > > > > > Jesus Ellin > > > Yuma Regional Medical Center > > > > > > ______________________________________________________________________ > > > This message is confidential, intended only for the named > > > recipient(s) and may contain information that is privileged > > > or exempt from disclosure under applicable law. If you are > > > not the intended recipient(s), you are notified that the > > > dissemination, distribution, or copying of this message is > > > strictly prohibited. If you receive this message in error, > > > or are not the named recipient(s), please notify the sender > > > at either the e-mail, fax, address, or telephone number > > > listed above and delete this e-mail from your computer. > > > Thank You. > > > ______________________________________________________________________ > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > CONFIDENTIALITY NOTICE > > This message and any included attachments are from Somerset Medical Center > > and are intended only for the addressee. The information contained in this > > message is confidential and may contain privileged, confidential, > > proprietary and/or trade secret information entitled to protection and/or > > exemption from disclosure under applicable law. Unauthorized forwarding, > > printing, copying, distribution, or use of such information is strictly > > prohibited and may be unlawful. If you are not the addressee, please > > promptly delete this message and notify the sender of the delivery error > > by e-mail or you may call Somerset Medical Center's computer Help Desk > > at 908-685-2200, ext. 4050. > > > > Be sure to visit Somerset Medical Center's Web site - > > www.somersetmedicalcenter.com - for the most up-to-date news, > > event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From alyssa <@t> alliedsearchpartners.com Wed Dec 22 10:25:59 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Dec 22 10:26:03 2010 Subject: [Histonet] Full Time Histo Opening in Ohio-Near Columbus Message-ID: *Position:* Histotechnician, Histotechnologist, Histology Technician, MOHS Technician *Location:* OH-About 25 Miles North of Columbus, Large Practice *Schedule:* Full Time, Direct (Permanent) Hire. Employee will be scheduled between 3-4 days when the MOHS surgeon is scheduled for MOHS. On the rare occasion he/she will be scheduled 2 days for MOHS surgery. On the days the MOHS surgeon is not doing MOHS, that time will be spent in the Dermpath Laboratory. Day Shift. *Environment: * *A* large comprehensive dermatology practice, established in 1980 who takes pride in providing high quality care and professional service. *Requirements:* - Ability to assist with CLIA documentation - Experience with both frozen sections & paraffin *preferred* - Candidates looking for long term employment - At least 2-5 years experience working with frozen sections for MOHS surgery & Paraffin. *Benefits:* * * Medical coverage that is paid 90% by the company, 10% by the employee. Dental and vision plans that can be obtained in addition Disability coverage that can be obtained in addition Company paid life insurance policy Paid Holidays Vacation Time 401K program that are always contributed to by doctors Sick time Personal Day(s) Uniform Reimbursement *To Apply:* * * To apply for this position please send resume to Alyssa@alliedsearchpartners.com , salary requirements, and availability to speak to one of our recruiters directly. Thank you!!! -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From wdesalvo.cac <@t> hotmail.com Wed Dec 22 10:40:05 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Dec 22 10:40:11 2010 Subject: [Histonet] RE: CMS Postpones Deadline for Physician Signatures In-Reply-To: References: , , , , Message-ID: I completely agree that both electronic and paper requisitions will require strict processes to ensure that either the hand written or electronic signature is secure and correct. Even after CMS "educates" us, their process and direction will probably be vague and will require all our best efforts. MERRY CHRISTMAs and have a WONDERFUL NEW YEAR!!! William DeSalvo, B.S., HTL(ASCP) Subject: Re: CMS Postpones Deadline for Physician Signatures Date: Wed, 22 Dec 2010 09:09:32 -0700 From: JEllin@yumaregional.org To: wdesalvo.cac@hotmail.com; trathborne@somerset-healthcare.com; jweems@sjha.org; histonet@lists.utsouthwestern.edu Yes we received the update. But there is still questions about difference from electronic ordering CPOE vs review and verify correct entry before proccessing by a clinician. Even though it states that this does not address electronic orders. We feel that this can be a problem for the future. Sent via DROID one Verizon Wireless -----Original message----- From: WILLIAM DESALVO To: trathborne@somerset-healthcare.com, Jesus Ellin , jweems@sjha.org, histonet Sent: Wed, Dec 22, 2010 15:44:34 GMT+00:00 Subject: CMS Postpones Deadline for Physician Signatures I have just received notice that the CMS requirement for Physician Signature Deadline of Jan 1, 2011 has been postponed. http://www.cms.gov/ClinicalLabFeeSched/ CMS has decided that in the first quarter of 2011 they will be developing educational and outreach materials to help educate all of us that are affected by the policy change. Looks like we have a little more time to figure this new process out. William DeSalvo, B.S., HTL(ASCP) > From: wdesalvo.cac@hotmail.com > To: trathborne@somerset-healthcare.com; jellin@yumaregional.org; jweems@sjha.org; histonet@lists.utsouthwestern.edu > Date: Fri, 17 Dec 2010 12:38:12 -0700 > Subject: RE: [Histonet] Physician Signatures > CC: > > > They sign into the case prior to surgery, they have Physician Assistant (PA) assigned to the case, a nurse is assigned to the case and they then enter additional information into the system after they complete surgery. It meets requirements. In Pathology, many of your Frozen Section specimens arrive in the lab on verbal orders or nursing applying a signature and we have processed for years. I believe the key point that you have to wrap your head around is that the electronic orders by the surgeon are complete before there is any delivery of results, the signed and preliminary or final report by the pathologist and that meets regulatory requirements. I have more concern on the clinical side. Orders can be entered, tests performed and results delivered more easily than in Pathology. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > Subject: RE: [Histonet] Physician Signatures > > Date: Fri, 17 Dec 2010 14:06:04 -0500 > > From: trathborne@somerset-healthcare.com > > To: wdesalvo.cac@hotmail.com; jellin@yumaregional.org; jweems@sjha.org; histonet@lists.utsouthwestern.edu > > > > So how do the surgeons electronically order a test? Do they order it before the procedure? What if they discover something and need to add an additional specimen? Our orders are electronically entered in the OR during the procedure. The surgeon is scrubbed and performing the procedure while the circulating nurse places the order. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of WILLIAM > > DESALVO > > Sent: Friday, December 17, 2010 1:56 PM > > To: Jesus Ellin; jweems@sjha.org; histonet > > Subject: RE: [Histonet] Physician Signatures > > > > > > > > I previously did not go into to deep detail about security and compliance, but in our system all computer portals are password protected, orders are signed with electronic signature of the ordering person and when a hard copy paper form is printed, the ordering physician's electronic signature is applied. It is the same type of process and signature that is used by the pathologist to electronically sign off all pathology reports. I absolutely agree that you must control access to ordering and maintain integrity in the system. As you work through your process to develop a more electronic system, you will have the opportunity to build in and require proper controls. With those controls implemented, you can then become confident that the electronic process can and will meet your regulatory requirements. > > > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > > > > > > > Subject: RE: [Histonet] Physician Signatures > > > Date: Fri, 17 Dec 2010 10:46:15 -0700 > > > From: JEllin@yumaregional.org > > > To: JWeems@sjha.org; wdesalvo.cac@hotmail.com; histonet@lists.utsouthwestern.edu > > > > > > This is true about electronic ordering but there is a provision on what > > > is acceptable for electronic order, just ordering in a system and > > > spitting out requisitions does not comply, There needs to be password > > > verification and review of this information by the clinician before it > > > is ordered and sent to the laboratory performing the service when the > > > requisition is created. They go on to say that orders and requisitions > > > are different aspects and should not be confused. IF the orders are just > > > received through an electronic interface then there is a difference. > > > But if the specimen is received using any paper form then this needs to > > > be signed by the clinician either electronically or manually. > > > > > > We are looking at this really closely, with using paper requisitions, > > > even orders from the OR for frozens, molecular studies, interoperative > > > work, all needs to be signed by the surgeon. Can you imagine the impact > > > that this is going to cause. > > > > > > Our current solutions is to have maybe standing orders in place and move > > > and look at process within the Main OR's, outpatient OR and clients. > > > Once again a huge problem,, but Bill is right this will lead to more and > > > more paperless enviroments. > > > > > > > > > Jesus Ellin > > > Yuma Regional Medical Center > > > > > > ______________________________________________________________________ > > > This message is confidential, intended only for the named > > > recipient(s) and may contain information that is privileged > > > or exempt from disclosure under applicable law. If you are > > > not the intended recipient(s), you are notified that the > > > dissemination, distribution, or copying of this message is > > > strictly prohibited. If you receive this message in error, > > > or are not the named recipient(s), please notify the sender > > > at either the e-mail, fax, address, or telephone number > > > listed above and delete this e-mail from your computer. > > > Thank You. > > > ______________________________________________________________________ > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > CONFIDENTIALITY NOTICE > > This message and any included attachments are from Somerset Medical Center > > and are intended only for the addressee. The information contained in this > > message is confidential and may contain privileged, confidential, > > proprietary and/or trade secret information entitled to protection and/or > > exemption from disclosure under applicable law. Unauthorized forwarding, > > printing, copying, distribution, or use of such information is strictly > > prohibited and may be unlawful. If you are not the addressee, please > > promptly delete this message and notify the sender of the delivery error > > by e-mail or you may call Somerset Medical Center's computer Help Desk > > at 908-685-2200, ext. 4050. > > > > Be sure to visit Somerset Medical Center's Web site - > > www.somersetmedicalcenter.com - for the most up-to-date news, > > event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From Rcartun <@t> harthosp.org Wed Dec 22 10:49:11 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Dec 22 10:49:19 2010 Subject: [Histonet] Fwd: Physician Signature Requirement Update Message-ID: <4D11E5B6.7400.0077.1@harthosp.org> FYI Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Denise Bologna 12/21/2010 5:27 PM >>> FYI >>> "CLMA" 12/21/2010 3:44 PM >>> ( http://www.clma.org/link.asp?ymlink=406069&finalurl=http%3A%2F%2Fwww%2Eclma%2Eorg ) Physician Signature Requirement Update Centers for Medicare & Medicaid Services (CMS) has issued a temporary (approximately three month) delay on the implementation of the Physician Signature Requirement. Please check back with CLMA ( http://www.clma.org/link.asp?ymlink=406069&finalurl=http%3A%2F%2Fwww%2Eclma%2Eorg ) often for updated information and read more ( http://www.clma.org/link.asp?ymlink=406069&finalurl=http%3A%2F%2Fwww%2Ecms%2Egov%2FClinicalLabFeeSched%2F ) at the CMS website. ( http://www.clma.org/link.asp?ymlink=406069&finalurl=http%3A%2F%2Fwww%2Eclma%2Eorg ) This email was sent to 'dbologn@clpct.com' from CLMA - Clinical Laboratory Management Association. If you wish to stop receiving email from us, you can simply remove yourself by visiting: http://clma.site-ym.com/general/opt.asp?e=dbologn@clpct.com From TJJ <@t> stowers.org Wed Dec 22 11:43:18 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Wed Dec 22 11:43:24 2010 Subject: [Histonet] Re: long term storage of tissue Message-ID: Melissa, I definitely would store them in 70% ethanol rather than leaving them in fixative for that time period. They should be fine for BrdU immunostaining after paraffin processing. Good luck with your studies! Teri From settembr <@t> umdnj.edu Wed Dec 22 12:18:22 2010 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Wed Dec 22 12:18:48 2010 Subject: [Histonet] Kathleen Dittrich, where are you? In-Reply-To: References: <38A56C4F4630D348A50B3720409270870E0FE287@dhmail.dhorg.org> <3201CF51728F6048A24FA3AFFFEEF1D317858318A7@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: Kathleen Dittrich, formerly from Dako. Where are you? Dana Settembre From Dorothy.L.Webb <@t> HealthPartners.Com Wed Dec 22 12:36:43 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Dec 22 12:36:50 2010 Subject: [Histonet] Physician signature Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43EA95058335@HPEMX3.HealthPartners.int> According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From JWeems <@t> sjha.org Wed Dec 22 12:38:30 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Dec 22 12:38:35 2010 Subject: [Histonet] RE: Physician signature In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43EA95058335@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43EA95058335@HPEMX3.HealthPartners.int> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332B22@CHEXCMS10.one.ads.che.org> It specifically mentions AP technical charges in the proposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, December 22, 2010 13:37 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Physician signature According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From thomas.crowell <@t> novartis.com Wed Dec 22 13:01:42 2010 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Wed Dec 22 13:01:51 2010 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 12/22/2010 and will not return until 01/03/2011. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From mpence <@t> grhs.net Wed Dec 22 13:25:20 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Dec 22 13:25:25 2010 Subject: [Histonet] RE: Physician signature In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164044B332B22@CHEXCMS10.one.ads.che.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974AD6@is-e2k3.grhs.net> And where would one go to see this proposal? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 12:39 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Physician signature It specifically mentions AP technical charges in the proposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, December 22, 2010 13:37 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Physician signature According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Dec 22 13:47:42 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Dec 22 13:47:46 2010 Subject: [Histonet] RE: Physician signature In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974AD6@is-e2k3.grhs.net> References: <92AD9B20A6C38C4587A9FEBE3A30E164044B332B22@CHEXCMS10.one.ads.che.org> <661949901A768E4F9CC16D8AF8F2838C03974AD6@is-e2k3.grhs.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332B65@CHEXCMS10.one.ads.che.org> The Federal Register is always a good place to find interesting reading. http://edocket.access.gpo.gov/2010/pdf/2010-15900.pdf Search for signature and you can find it...starts on page 40161. In the middle column on page 40162 charges for TC for AP are mentioned. consistent with the principle in ? 410.32(a) that a written order for diagnostic tests including those paid under the CLFS and those that are not paid under the CLFS (for example, that are paid under the PFS or under the OPPS), such as X-rays, MRIs, and the TC of physician pathology services, must be signed by the ordering physician or NPP. That is, the policy that signatures are not required on requisitions for clinical diagnostic laboratory tests paid based on the CLFS applies only to requisitions (as opposed to written orders) (74 FR 33642). Blah blah blah.... At the end they say "We believe that this policy would result in a less confusing process." Merry Christmas, Everyone! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, December 22, 2010 14:25 To: Weems, Joyce; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature And where would one go to see this proposal? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 12:39 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Physician signature It specifically mentions AP technical charges in the proposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, December 22, 2010 13:37 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Physician signature According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From alisha <@t> ka-recruiting.com Wed Dec 22 13:48:21 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Dec 22 13:48:20 2010 Subject: [Histonet] High Paying Job Opportunity - Live anywhere Message-ID: <1222998903.1293047301530.JavaMail.cfservice@sl4app2> Hi Histonet Members, I am a healthcare recruiter, specifically focused on the laboratory/pathology space. I have openings all across the country and would be interested in speaking with anyone interested in searching for a new job. I am currently working on an amazing opportunity with a cancer diagnostics company. This company is looking to hire on Technical Support Specialists in several areas across the country. They currently are looking for people located anywhere West of the Mississippi River or in Atlanta, GA or in Nashville, TN or in New York City or in Philadelphia. The TSS would be responsible for training pathologists, surgeons, radiologists, and pathologist assistant's to ensure adequate sampling on breast cancer and colon cancer tissues. They are looking for someone with a Bachelor's Degree and 5+ years experience in tissue grossing/sampling, histology, or in a pathology or surgical capacity. The ideal candidate with be PA(ASCP) or HTL(ASCP) certified. This position requires extensive travel, paid for by the company. The compensation package is fantastic with a high base salary and up to a 20% bonus. If interested in learning more details, please email me at alisha@ka-recruiting.com. If you are not interested but know of someone you could recommend for this position, please pass on their contact information to me. We have a referral bonus for anyone you refer to me that I place into a new job. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From mward <@t> wfubmc.edu Wed Dec 22 14:23:54 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Wed Dec 22 14:24:10 2010 Subject: [Histonet] Fisher MicroProbe manual staining system Message-ID: Does anyone have one of these systems that they would be willing to donate? One of our pathologists is going on a mission trip to Honduras and would like to take one of these down there with him. We used to have the set up but recently got rid of it. (Timing is everything!!) Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From Timothy.Morken <@t> ucsfmedctr.org Wed Dec 22 14:33:14 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Dec 22 14:33:30 2010 Subject: [Histonet] RE: Physician signature In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164044B332B65@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E164044B332B22@CHEXCMS10.one.ads.che.org> <661949901A768E4F9CC16D8AF8F2838C03974AD6@is-e2k3.grhs.net> <92AD9B20A6C38C4587A9FEBE3A30E164044B332B65@CHEXCMS10.one.ads.che.org> Message-ID: Joyce wrote: "The Federal Register is always a good place to find interesting reading" Gee, Joyce, all these years I've known you and I never realized you are a masochist!! But maybe the fact you are the go-to person for regulations should have been a clue!;) Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 11:48 AM To: Mike Pence; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature The Federal Register is always a good place to find interesting reading. http://edocket.access.gpo.gov/2010/pdf/2010-15900.pdf Search for signature and you can find it...starts on page 40161. In the middle column on page 40162 charges for TC for AP are mentioned. consistent with the principle in ? 410.32(a) that a written order for diagnostic tests including those paid under the CLFS and those that are not paid under the CLFS (for example, that are paid under the PFS or under the OPPS), such as X-rays, MRIs, and the TC of physician pathology services, must be signed by the ordering physician or NPP. That is, the policy that signatures are not required on requisitions for clinical diagnostic laboratory tests paid based on the CLFS applies only to requisitions (as opposed to written orders) (74 FR 33642). Blah blah blah.... At the end they say "We believe that this policy would result in a less confusing process." Merry Christmas, Everyone! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, December 22, 2010 14:25 To: Weems, Joyce; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature And where would one go to see this proposal? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 12:39 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Physician signature It specifically mentions AP technical charges in the proposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, December 22, 2010 13:37 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Physician signature According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley <@t> sanfordburnham.org Wed Dec 22 14:36:39 2010 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Wed Dec 22 14:36:44 2010 Subject: [Histonet] Frozen Specimen Handling Message-ID: Hi HistoLand, I have a question regarding frozen specimen handling. I have a group that gave me hearts, brown fat and white fat and there are some issues with the morphology of the tissue and also the cutting quality. Unfortunately I was part of the whole process from tissue harvesting to cutting and staining of specimens. We placed harvested tissue in a cryo mold with OCT and snap froze the block in LN2 and then placed on dry ice. I know sometimes the quick freeze can cause some effects but was wondering how others are addressing the freezing of tissue, especially fat. We have frozen fat this way before and did not have the same issues. Any help or suggestions would be great. Thanks!!! Kind Regards! John Shelley From JWeems <@t> sjha.org Wed Dec 22 14:43:19 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Dec 22 14:43:22 2010 Subject: [Histonet] RE: Physician signature In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E164044B332B22@CHEXCMS10.one.ads.che.org> <661949901A768E4F9CC16D8AF8F2838C03974AD6@is-e2k3.grhs.net> <92AD9B20A6C38C4587A9FEBE3A30E164044B332B65@CHEXCMS10.one.ads.che.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332B98@CHEXCMS10.one.ads.che.org> Oh dear, now you all know! You all ought to see where our tax dollars are going!! Such a convoluted mess. I don't know how the writer can understand what he/she is saying! We could save so may trees if they would just get to the point! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Wednesday, December 22, 2010 15:33 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature Joyce wrote: "The Federal Register is always a good place to find interesting reading" Gee, Joyce, all these years I've known you and I never realized you are a masochist!! But maybe the fact you are the go-to person for regulations should have been a clue!;) Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 11:48 AM To: Mike Pence; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature The Federal Register is always a good place to find interesting reading. http://edocket.access.gpo.gov/2010/pdf/2010-15900.pdf Search for signature and you can find it...starts on page 40161. In the middle column on page 40162 charges for TC for AP are mentioned. consistent with the principle in ? 410.32(a) that a written order for diagnostic tests including those paid under the CLFS and those that are not paid under the CLFS (for example, that are paid under the PFS or under the OPPS), such as X-rays, MRIs, and the TC of physician pathology services, must be signed by the ordering physician or NPP. That is, the policy that signatures are not required on requisitions for clinical diagnostic laboratory tests paid based on the CLFS applies only to requisitions (as opposed to written orders) (74 FR 33642). Blah blah blah.... At the end they say "We believe that this policy would result in a less confusing process." Merry Christmas, Everyone! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, December 22, 2010 14:25 To: Weems, Joyce; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature And where would one go to see this proposal? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 12:39 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Physician signature It specifically mentions AP technical charges in the proposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, December 22, 2010 13:37 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Physician signature According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From CThornton <@t> dahlchase.com Wed Dec 22 15:08:20 2010 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed Dec 22 15:08:25 2010 Subject: [Histonet] Symphony cover slipper Message-ID: Hello, Anyone else out there have issues with misaligned cover slips on the Symphony stainer? We have two stainers and there doesn't seem to be a pattern. Sometimes it's one stainer, sometimes the other, sometimes every tray, sometimes one out of ten trays. Usually the cover slip is off just enough to have a sharp edge and we need to fix it, which for any other Symphony owners you know that's a pain. Whenever they adjust the cover slipper, it will work well for a day or two then start acting up again. Is it just us? Thanks! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From 41dmb41 <@t> gmail.com Wed Dec 22 15:09:00 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Wed Dec 22 15:09:27 2010 Subject: [Histonet] VIP 1000 Help Message-ID: We've got the old workhorse, the VIP 1000, that we still use. During a move, the guide, which shows all the commands you can do with that magnet, got lost. Is there anyone out there that has this and could send or fax me a copy? It would be much appreciated! Thanks, Drew Meyer -- ~Love is like a booger. You keep picking at it until you get it, then wonder what to do with it. From liz <@t> premierlab.com Wed Dec 22 16:18:18 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Dec 22 16:15:35 2010 Subject: [Histonet] FW: ASCP Action Alert - Physician Signature Requirement _FYI References: Message-ID: FYI - saw alot about this today, just got this e-mail from ASCP Liz -----Original Message----- From: American Society for Clinical Pathology [mailto:ascp@site1.ascpmail.org] Sent: Wed 12/22/2010 2:42 PM To: Liz Chlipala Subject: ASCP Action Alert - Physician Signature Requirement Physician Signature Requirement Burdens Labs, Jeopardizes Patients ASCP urges you to act before the Jan. 3 deadline Recently, the Centers for Medicare and Medicaid Services (CMS) finalized its Medicare Physician Fee Schedule final rule for the 2011 calendar year. The rule includes a troubling policy change requiring a physician's or qualified nonphysician practitioner's (NPP) signature on requisitions for clinical diagnostic laboratory tests paid under the clinical laboratory fee schedule (effective Jan. 1, 2011). CMS defines a requisition as "the actual paperwork, such as a form, which is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient." Currently, a physician signature is required only on orders for laboratory services. ASCP believes the new rule could adversely affect patient care and complicate the provision of the laboratory services. In cases where a signature from the ordering physician or NPP is absent, laboratories could be left scrambling trying to obtain the signature. Late Breaking News Rule: Recently CMS has partially agreed to a delayed implementation request from ASCP and other members of the clinical laboratory coalition organizations. CMS will delay implementation of the rule for three months. During this period, ASCP will continue to pressure CMS to withdraw its new proposal. ASCP urges you to contact CMS and tell them of your opposition to the policy change. Contact CMS before the Jan. 3 Comment Deadline to maximize the effectiveness of your voice. Read more and take action today . . . Tell a friend: Not everyone receives ASCP's Action Alerts, so please forward this message to your peers and co-workers! 2010 American Society for Clinical Pathology 33 West Monroe Street, Suite 1600, Chicago, IL 60603 312.541.4999 ~ info@ascp.org From sgoebel <@t> mirnarx.com Wed Dec 22 16:52:13 2010 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Dec 22 16:52:17 2010 Subject: [Histonet] Frozen Specimen Handling In-Reply-To: References: Message-ID: Cutting frozen fat is virtually impossible. There are a few articles I have seen online (one is called something along the lines of "the fat demon" or something pun-tastic like that). Because fat is so oily it usually doesn't freeze at all. If you have a lot of fat in the tissue you are trying to freeze and not just a big hunk of fat (say an inguinal LN incased in fat), cutting the fatty tissue can be a challenge too. I have heard you can try and cut it thicker, but some doc's don't like that. Another thing we tried was fixing the fat for about 15 minutes in Pen-Fix (or some other alcoholic formalin mixture), if you have this much time then that can also help. Good Luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Wednesday, December 22, 2010 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Specimen Handling Hi HistoLand, I have a question regarding frozen specimen handling. I have a group that gave me hearts, brown fat and white fat and there are some issues with the morphology of the tissue and also the cutting quality. Unfortunately I was part of the whole process from tissue harvesting to cutting and staining of specimens. We placed harvested tissue in a cryo mold with OCT and snap froze the block in LN2 and then placed on dry ice. I know sometimes the quick freeze can cause some effects but was wondering how others are addressing the freezing of tissue, especially fat. We have frozen fat this way before and did not have the same issues. Any help or suggestions would be great. Thanks!!! Kind Regards! John Shelley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alineumann <@t> aol.com Wed Dec 22 17:06:19 2010 From: alineumann <@t> aol.com (alineumann@aol.com) Date: Wed Dec 22 17:06:29 2010 Subject: [Histonet] Used embedding center and microtome Message-ID: <8CD7043FA4CB24F-478-1572A@Webmail-d112.sysops.aol.com> Hello Histonetters, especially those in the Denver area, we have the following available: ? A functional?embedding center, Reichert Histostat 8034, purchased in 1/2002,?used,?for $1500, with manual.? Will sell for $700, or $1100 if shipping is required (ie, if you cannot pick it up from our Denver suburb location).. ? A functional microtome, Leitz 1512,??purchased used in 10/2004 for $1500.??We can sell for $700, or $850 if shipping is required.? Pay Pal and corporate checks accepted.? Please contact me at the email or phone below if interested.? Thank you, Alice Neumann MD Western Wyoming Pathology PC Pinnacle Pathology PC 9423 West Kentucky Place Lakewood, CO?? 80226 24 Hour Cell Phone:? 307-413-4092 alineumann@aol.com? ? ? From victor <@t> pathology.washington.edu Wed Dec 22 17:16:37 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Dec 22 17:17:03 2010 Subject: [Histonet] CAP Question regarding procedure manual Message-ID: <4D1286D5.9030207@pathology.washington.edu> Is there a requirement to have a signature of every staff member on a procedure if they perform that procedure in a manual? Wouldn't one signature on a cover page suffice that you have read and understand what is in the manual? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From JWeems <@t> sjha.org Wed Dec 22 17:34:54 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Dec 22 17:34:59 2010 Subject: [Histonet] CAP Question regarding procedure manual In-Reply-To: <4D1286D5.9030207@pathology.washington.edu> References: <4D1286D5.9030207@pathology.washington.edu> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332BCB@CHEXCMS10.one.ads.che.org> Please tell me that is good enough! We do that here - for each manual. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, December 22, 2010 18:17 To: Histonet Subject: [Histonet] CAP Question regarding procedure manual Is there a requirement to have a signature of every staff member on a procedure if they perform that procedure in a manual? Wouldn't one signature on a cover page suffice that you have read and understand what is in the manual? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From wdesalvo.cac <@t> hotmail.com Wed Dec 22 17:46:09 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Dec 22 17:46:13 2010 Subject: [Histonet] CAP Question regarding procedure manual In-Reply-To: <4D1286D5.9030207@pathology.washington.edu> References: <4D1286D5.9030207@pathology.washington.edu> Message-ID: You will need a signature for the manual and for each procedure that is implemented or amended during your cycle year. If your manual is very stable, a yearly signature page for the entire manual may suffice, but the inspector will note the date everyone signed the manual review page and then go through the manual to see if there are any implementation dates or procedure change dates after the manual review date. Each occurrence found one can be a deficiency. In my lab, it is not uncommon for new procedures to be written and implemented or existing procedures implemented during a cycle year and all these events require a signature by System Medical Director, Site Medical Director, Site Lab Director, System Technical Specialist (responsible for physically writing) and all employees affected. You must prove to CAP that all personnel know where the procedure manual is located, that they have reviewed the manual as a whole and that they have reviewed all additions implemented. We have employees sign each technical alert, procedure update/change and new procedures, along with a once a year review of all manuals. This type of signature trail is part of and right in line with quality management and document control. This process alone can be a daunting task and requires a lot or forethought. To better manage this process, we are moving to an electronic on-line, web based procedure manual with document control functions that tracks usage by employee and captures electronic signatures for each step of the creation, review and implementation process. Thankfully, this process is the responsibility of our Technical Specialist for Anatomic Pathology and our System Quality Department. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 22 Dec 2010 15:16:37 -0800 > From: victor@pathology.washington.edu > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] CAP Question regarding procedure manual > > Is there a requirement to have a signature of every staff member on a > procedure if they perform that procedure in a manual? Wouldn't one > signature on a cover page suffice that you have read and understand what > is in the manual? > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Wed Dec 22 17:57:52 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Dec 22 18:06:20 2010 Subject: [Histonet] CAP Question regarding procedure manual In-Reply-To: Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C6AEA@EXCHANGECLUSTER.yumaregional.local> Currently Victor we use to have the techs sign off on all the policies and procedures. As Bill stated this is a big pain. We are now all electonic and have gone live with I-Passport our document control software,, this has saved tons of time and effort. Electronic is the way to go especially for updates and new information. Users are notifed by email and it allows the supervisor to keep track of edits and much more. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, December 22, 2010 4:46 PM To: victor@pathology.washington.edu; histonet Subject: RE: [Histonet] CAP Question regarding procedure manual You will need a signature for the manual and for each procedure that is implemented or amended during your cycle year. If your manual is very stable, a yearly signature page for the entire manual may suffice, but the inspector will note the date everyone signed the manual review page and then go through the manual to see if there are any implementation dates or procedure change dates after the manual review date. Each occurrence found one can be a deficiency. In my lab, it is not uncommon for new procedures to be written and implemented or existing procedures implemented during a cycle year and all these events require a signature by System Medical Director, Site Medical Director, Site Lab Director, System Technical Specialist (responsible for physically writing) and all employees affected. You must prove to CAP that all personnel know where the procedure manual is located, that they have reviewed the manual as a whole and that they have reviewed all additions implemented. We have employees sign each technical alert, procedure update/change and new procedures, along with a once a year review of all manuals. This type of signature trail is part of and right in line with quality management and document control. This process alone can be a daunting task and requires a lot or forethought. To better manage this process, we are moving to an electronic on-line, web based procedure manual with document control functions that tracks usage by employee and captures electronic signatures for each step of the creation, review and implementation process. Thankfully, this process is the responsibility of our Technical Specialist for Anatomic Pathology and our System Quality Department. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 22 Dec 2010 15:16:37 -0800 > From: victor@pathology.washington.edu > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] CAP Question regarding procedure manual > > Is there a requirement to have a signature of every staff member on a > procedure if they perform that procedure in a manual? Wouldn't one > signature on a cover page suffice that you have read and understand > what is in the manual? > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From BSullivan <@t> shorememorial.org Thu Dec 23 06:53:22 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Thu Dec 23 06:56:36 2010 Subject: [Histonet] CAP Question regarding procedure manual In-Reply-To: <4D1286D5.9030207@pathology.washington.edu> Message-ID: Victor, To my knowledge all you need is proof that the staff reviewed the manuals. We accomplish this by a sign off sheet in the front of each manual we use. The Supervisor, or designee, needs to review and sign off on each procedure in each manual. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Victor Tobias To Sent by: Histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] CAP Question regarding 12/22/2010 06:16 procedure manual PM Is there a requirement to have a signature of every staff member on a procedure if they perform that procedure in a manual? Wouldn't one signature on a cover page suffice that you have read and understand what is in the manual? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Thu Dec 23 07:26:40 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu Dec 23 07:26:44 2010 Subject: [Histonet] CAP Question regarding procedure manual In-Reply-To: References: <4D1286D5.9030207@pathology.washington.edu>, Message-ID: Beatrice, How do you show proof to CAP that your employees that use the procedures and perform the tasks described in the procedures have reviewed and understand the procedure when there is a new one during your cycle year and before it is implemented? CAP has changed the way they inspect and they are now looking for how whether the employees performing a task described in the procedure understands the procedure and is performing exactly as the procedure describes. They are not as concerned that the Supervisor and management knows about the procedures, they want to see how informed, trained and competent the bench employee is. William DeSalvo, B.S., HTL(ASCP) > To: victor@pathology.washington.edu > From: BSullivan@shorememorial.org > Date: Thu, 23 Dec 2010 07:53:22 -0500 > Subject: Re: [Histonet] CAP Question regarding procedure manual > CC: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > Victor, > To my knowledge all you need is proof that the staff reviewed the manuals. > We accomplish this by a sign off sheet in the front of each manual we use. > The Supervisor, or designee, needs to review and sign off on each procedure > in each manual. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > Speak only well of people and you need never whisper > > > > Victor Tobias > .washington.edu> To > Sent by: Histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] CAP Question regarding > 12/22/2010 06:16 procedure manual > PM > > > > > > > > > > Is there a requirement to have a signature of every staff member on a > procedure if they perform that procedure in a manual? Wouldn't one > signature on a cover page suffice that you have read and understand what > is in the manual? > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vkline2344 <@t> comcast.net Thu Dec 23 08:14:01 2010 From: vkline2344 <@t> comcast.net (vkline2344) Date: Thu Dec 23 08:14:25 2010 Subject: [Histonet] Materials to prepare for the registry exam? Message-ID: <11FD5E30DE9042EE94C341A6A2CE9114@vicPC> I was curious if anyone has ever used the Flashcards based on the Carson book to prepare for the registry exam and if so, if you recommend them or not? I have the Board of Registry Study Guide, but was thinking of additional materials to have on hand for when I start to prepare for the registry exam. I'm currently in school and plan to graduate in June and would like to take my exam soon after. Thanks so much and Happy Holidays! Victoria From ROrr <@t> northshore.org Thu Dec 23 08:21:18 2010 From: ROrr <@t> northshore.org (Orr, Rebecca) Date: Thu Dec 23 08:21:22 2010 Subject: [Histonet] if you could pick your own floor surface... In-Reply-To: <7e1a87cb-ee1a-48b9-8a4e-8f76350caa6e@EXCHCAS02.enhnet.org> Message-ID: Hi Friends, What type of floor surface would you pick if you could design your own histology lab? I was walking through Costco the other night and noticed theirs is just concrete. Compensating for leg fatigue on the Assistants who have to stand, I'm thinking this type of surface might be easiest to keep clean. What do you think about just concrete? Would there need to be a paraffin scrape nightly? Would that be enough? If paraffin is tracked in the morning will this type of floor be slippy (Pittsburgh slang) by 3pm? What flooring do you have and what drawbacks and advantages do you see with it? Many thanks and no skids! Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ********* Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. From jsands <@t> innovishealth.com Thu Dec 23 08:35:32 2010 From: jsands <@t> innovishealth.com (Sands, Jenn) Date: Thu Dec 23 08:35:38 2010 Subject: [Histonet] Materials to prepare for the registry exam? In-Reply-To: <11FD5E30DE9042EE94C341A6A2CE9114@vicPC> References: <11FD5E30DE9042EE94C341A6A2CE9114@vicPC> Message-ID: <3F317E4D03ED254183E9FB1840E71F38015CCF55@Exchange01.dakcl.com> I used them to take my HTL exam. I found them quite helpful because not only do they give you the correct answer, but they explain why it is correct and why the others are not. Good Luck. Jenn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of vkline2344 Sent: Thursday, December 23, 2010 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Materials to prepare for the registry exam? I was curious if anyone has ever used the Flashcards based on the Carson book to prepare for the registry exam and if so, if you recommend them or not? I have the Board of Registry Study Guide, but was thinking of additional materials to have on hand for when I start to prepare for the registry exam. I'm currently in school and plan to graduate in June and would like to take my exam soon after. Thanks so much and Happy Holidays! Victoria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------ This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From BSullivan <@t> shorememorial.org Thu Dec 23 08:55:37 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Thu Dec 23 08:58:59 2010 Subject: [Histonet] CAP Question regarding procedure manual In-Reply-To: Message-ID: When there is a change or new procedure made part of the existing manual it is noted on a separate sheet designated for this. After this new or changed procedure is signed off by the Medical Director it is given to the rest of the department to read. This is initialed by each person on that sheet. These sheets are within the manuals. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper WILLIAM DESALVO To , 12/23/2010 08:26 Victor Tobias AM cc histonet , Subject RE: [Histonet] CAP Question regarding procedure manual Beatrice, How do you show proof to CAP that your employees that use the procedures and perform the tasks described in the procedures have reviewed and understand the procedure when there is a new one during your cycle year and before it is implemented? CAP has changed the way they inspect and they are now looking for how whether the employees performing a task described in the procedure understands the procedure and is performing exactly as the procedure describes. They are not as concerned that the Supervisor and management knows about the procedures, they want to see how informed, trained and competent the bench employee is. William DeSalvo, B.S., HTL(ASCP) > To: victor@pathology.washington.edu > From: BSullivan@shorememorial.org > Date: Thu, 23 Dec 2010 07:53:22 -0500 > Subject: Re: [Histonet] CAP Question regarding procedure manual > CC: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > Victor, > To my knowledge all you need is proof that the staff reviewed the manuals. > We accomplish this by a sign off sheet in the front of each manual we use. > The Supervisor, or designee, needs to review and sign off on each procedure > in each manual. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > Speak only well of people and you need never whisper > > > > Victor Tobias > .washington.edu> To > Sent by: Histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] CAP Question regarding > 12/22/2010 06:16 procedure manual > PM > > > > > > > > > > Is there a requirement to have a signature of every staff member on a > procedure if they perform that procedure in a manual? Wouldn't one > signature on a cover page suffice that you have read and understand what > is in the manual? > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Thu Dec 23 09:50:22 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Dec 23 09:50:25 2010 Subject: [Histonet] if you could pick your own floor surface... In-Reply-To: References: <7e1a87cb-ee1a-48b9-8a4e-8f76350caa6e@EXCHCAS02.enhnet.org> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC2466969F@exchange.cmc-nh.org> Hi Rebecca, We built a Pathology lab from scratch last year and this was a question that we explored when deciding on flooring materials. We ended up choosing poured epoxy (with rubberized compounds embedded) over concrete. The floor is seamless, wax and slip and stain resistant, and very easy to keep clean. We do a minimum of scrapping a the end of each day. At the end of a year, the floor has stood up extremely well and looks great. It costs a little more up front but you end up saving in the costs of keeping it clean and wax free and in replacement costs. It is estimated that we will not have to look at replacing it for at least 10 years. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Thursday, December 23, 2010 9:21 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] if you could pick your own floor surface... Hi Friends, What type of floor surface would you pick if you could design your own histology lab? I was walking through Costco the other night and noticed theirs is just concrete. Compensating for leg fatigue on the Assistants who have to stand, I'm thinking this type of surface might be easiest to keep clean. What do you think about just concrete? Would there need to be a paraffin scrape nightly? Would that be enough? If paraffin is tracked in the morning will this type of floor be slippy (Pittsburgh slang) by 3pm? What flooring do you have and what drawbacks and advantages do you see with it? Many thanks and no skids! Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ********* Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Thu Dec 23 10:49:53 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu Dec 23 10:49:58 2010 Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject In-Reply-To: <572D1F45B44B3D4096D554B4CB40639C032034E8BF@EXCHCCRMB.schosp.org> References: <4D1286D5.9030207@pathology.washington.edu>, , , <572D1F45B44B3D4096D554B4CB40639C032034E8BF@EXCHCCRMB.schosp.org> Message-ID: Thanks, I am aware of Peggy's form. We have similar hard copy forms now, but we are moving to an electronic system. It will be much more manageable for 125 employees. When you state in your form the employee is competent, do you have documentation to support that there was a competency evaluation? We only state the employee has read and understands. We have separate documentation for training and competency. I love this type of discussion string. There are so many different processes out in Histoland. Do others document separately the original creation, training, competency, implementation of procedures/processes and then continual yearly training, competency and review of those processes? Are you using a Quality Management System? How about Electronic system to manage? William DeSalvo, B.S., HTL(ASCP) > From: RCazares@schosp.org > To: wdesalvo.cac@hotmail.com > Date: Thu, 23 Dec 2010 10:17:48 -0600 > Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > Hello histonetters, > > When I implement a new procedure in my lab, besides the routine yearly sign-off sheet that accompanies the procedure, there an additional sheet that states > > " The following employees have reviewed this procedure and by signing below are acknowledging that they understand and are competent in performing the procedure:" > > Below that statement is a box with signature and date lines for all the employees using this procedure to sign. This makes each employee responsible for reading and understanding the procedure. This I got from one of Peggy Wenk's workshops (Thanks Peggy!!). I can send a copy to anyone who'd like one, just contact me directly. > > > Ruth Cazares, HT (ASCP) > Histology Supervisor > Department of Pathology > Swedish Covenant Hospital > Chicago, IL > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Thursday, December 23, 2010 7:27 AM > To: bsullivan@shorememorial.org; Victor Tobias > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > > Beatrice, > How do you show proof to CAP that your employees that use the procedures and perform the tasks described in the procedures have reviewed and understand the procedure when there is a new one during your cycle year and before it is implemented? > > CAP has changed the way they inspect and they are now looking for how whether the employees performing a task described in the procedure understands the procedure and is performing exactly as the procedure describes. They are not as concerned that the Supervisor and management knows about the procedures, they want to see how informed, trained and competent the bench employee is. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > To: victor@pathology.washington.edu > > From: BSullivan@shorememorial.org > > Date: Thu, 23 Dec 2010 07:53:22 -0500 > > Subject: Re: [Histonet] CAP Question regarding procedure manual > > CC: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > > > Victor, > > To my knowledge all you need is proof that the staff reviewed the manuals. > > We accomplish this by a sign off sheet in the front of each manual we use. > > The Supervisor, or designee, needs to review and sign off on each procedure > > in each manual. > > > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > > AP Supervisor > > Shore Memorial Hospital > > 609-653-3590 > > > > > > Speak only well of people and you need never whisper > > > > > > > > Victor Tobias > > > .washington.edu> To > > Sent by: Histonet > > histonet-bounces@ > > lists.utsouthwest cc > > ern.edu > > Subject > > [Histonet] CAP Question regarding > > 12/22/2010 06:16 procedure manual > > PM > > > > > > > > > > > > > > > > > > > > Is there a requirement to have a signature of every staff member on a > > procedure if they perform that procedure in a manual? Wouldn't one > > signature on a cover page suffice that you have read and understand what > > is in the manual? > > > > Victor > > > > -- > > Victor Tobias > > Clinical Applications Analyst > > University of Washington Medical Center > > Dept of Pathology Room BB220 > > 1959 NE Pacific > > Seattle, WA 98195 > > victor@pathology.washington.edu > > 206-598-2792 > > 206-598-7659 Fax > > ================================================= > > Privileged, confidential or patient identifiable information may be > > contained in this message. This information is meant only for the use > > of the intended recipients. If you are not the intended recipient, or > > if the message has been addressed to you in error, do not read, > > disclose, reproduce, distribute, disseminate or otherwise use this > > transmission. Instead, please notify the sender by reply e-mail, and > > then destroy all copies of the message and any attachments. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > > Thank you for your cooperation. From jaylundgren <@t> gmail.com Thu Dec 23 10:59:50 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Dec 23 10:59:54 2010 Subject: [Histonet] Used embedding center and microtome In-Reply-To: <8CD7043FA4CB24F-478-1572A@Webmail-d112.sysops.aol.com> References: <8CD7043FA4CB24F-478-1572A@Webmail-d112.sysops.aol.com> Message-ID: ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RCazares <@t> schosp.org Thu Dec 23 11:04:16 2010 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Thu Dec 23 11:04:23 2010 Subject: [SPAM-HC] - RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject - Email found in subject In-Reply-To: References: <4D1286D5.9030207@pathology.washington.edu>, , , <572D1F45B44B3D4096D554B4CB40639C032034E8BF@EXCHCCRMB.schosp.org> Message-ID: <572D1F45B44B3D4096D554B4CB40639C032034E8C1@EXCHCCRMB.schosp.org> Hi William, Our competency evaluation is done once a year as part of the employee's annual evaluation. I don't actually have each employee perform the procedure as part of the documentation, although................. hmmmm, maybe an in-service to discuss the new procedure with all employees? I agree with you about discussing things on Histonet, I have found it to be a lifesaver on more than one occasion (OK, so maybe that's a little dramatic, but you get the point) :) Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Thursday, December 23, 2010 10:50 AM To: Cazares, Ruth; histonet Subject: [SPAM-HC] - RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject - Email found in subject Thanks, I am aware of Peggy's form. We have similar hard copy forms now, but we are moving to an electronic system. It will be much more manageable for 125 employees. When you state in your form the employee is competent, do you have documentation to support that there was a competency evaluation? We only state the employee has read and understands. We have separate documentation for training and competency. I love this type of discussion string. There are so many different processes out in Histoland. Do others document separately the original creation, training, competency, implementation of procedures/processes and then continual yearly training, competency and review of those processes? Are you using a Quality Management System? How about Electronic system to manage? William DeSalvo, B.S., HTL(ASCP) > From: RCazares@schosp.org > To: wdesalvo.cac@hotmail.com > Date: Thu, 23 Dec 2010 10:17:48 -0600 > Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > Hello histonetters, > > When I implement a new procedure in my lab, besides the routine yearly sign-off sheet that accompanies the procedure, there an additional sheet that states > > " The following employees have reviewed this procedure and by signing below are acknowledging that they understand and are competent in performing the procedure:" > > Below that statement is a box with signature and date lines for all the employees using this procedure to sign. This makes each employee responsible for reading and understanding the procedure. This I got from one of Peggy Wenk's workshops (Thanks Peggy!!). I can send a copy to anyone who'd like one, just contact me directly. > > > Ruth Cazares, HT (ASCP) > Histology Supervisor > Department of Pathology > Swedish Covenant Hospital > Chicago, IL > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Thursday, December 23, 2010 7:27 AM > To: bsullivan@shorememorial.org; Victor Tobias > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > > Beatrice, > How do you show proof to CAP that your employees that use the procedures and perform the tasks described in the procedures have reviewed and understand the procedure when there is a new one during your cycle year and before it is implemented? > > CAP has changed the way they inspect and they are now looking for how whether the employees performing a task described in the procedure understands the procedure and is performing exactly as the procedure describes. They are not as concerned that the Supervisor and management knows about the procedures, they want to see how informed, trained and competent the bench employee is. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > To: victor@pathology.washington.edu > > From: BSullivan@shorememorial.org > > Date: Thu, 23 Dec 2010 07:53:22 -0500 > > Subject: Re: [Histonet] CAP Question regarding procedure manual > > CC: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > > > Victor, > > To my knowledge all you need is proof that the staff reviewed the manuals. > > We accomplish this by a sign off sheet in the front of each manual we use. > > The Supervisor, or designee, needs to review and sign off on each procedure > > in each manual. > > > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > > AP Supervisor > > Shore Memorial Hospital > > 609-653-3590 > > > > > > Speak only well of people and you need never whisper > > > > > > > > Victor Tobias > > > .washington.edu> To > > Sent by: Histonet > > histonet-bounces@ > > lists.utsouthwest cc > > ern.edu > > Subject > > [Histonet] CAP Question regarding > > 12/22/2010 06:16 procedure manual > > PM > > > > > > > > > > > > > > > > > > > > Is there a requirement to have a signature of every staff member on a > > procedure if they perform that procedure in a manual? Wouldn't one > > signature on a cover page suffice that you have read and understand what > > is in the manual? > > > > Victor > > > > -- > > Victor Tobias > > Clinical Applications Analyst > > University of Washington Medical Center > > Dept of Pathology Room BB220 > > 1959 NE Pacific > > Seattle, WA 98195 > > victor@pathology.washington.edu > > 206-598-2792 > > 206-598-7659 Fax > > ================================================= > > Privileged, confidential or patient identifiable information may be > > contained in this message. This information is meant only for the use > > of the intended recipients. If you are not the intended recipient, or > > if the message has been addressed to you in error, do not read, > > disclose, reproduce, distribute, disseminate or otherwise use this > > transmission. Instead, please notify the sender by reply e-mail, and > > then destroy all copies of the message and any attachments. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > > Thank you for your cooperation. ________________________________ *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From Dorothy.L.Webb <@t> HealthPartners.Com Thu Dec 23 11:08:27 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Dec 23 11:08:31 2010 Subject: [Histonet] Parathyroid staining Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43EA95058346@HPEMX3.HealthPartners.int> Does anyone use Toluidine blue to stain parathyroids when they come in fresh for a frozen section? One of our pathologist heard of this and I am not familiar with it. I have heard of it with the Paragon stain, but not certain what percentage would be needed if only Toluidine blue was used. Thanks ahead of time and Happy Holidays!! Dorothy Webb, HT ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From Erik.Dokken <@t> onassignment.com Thu Dec 23 12:20:16 2010 From: Erik.Dokken <@t> onassignment.com (Erik Dokken) Date: Thu Dec 23 12:20:18 2010 Subject: [Histonet] RE: Histonet Digest, Vol 85, Issue 22 In-Reply-To: <201012231808.oBNI85LC001478@smtp12.onasgn.net> References: <201012231808.oBNI85LC001478@smtp12.onasgn.net> Message-ID: On Assignment Healthcare has an immediate opportunity for a qualified Histo Tech in the Seattle area. This is a full time 4 month contract assignment with the opportunity to extend. Our client is a large Dermatology Practice in the Seattle Area. Duties and Responsibilities include: - Provide quality tissue preparations from grossing, processing, embedding, cutting and staining tissue specimens from all patient populations according to current department protocols. - Perform, document and review quality control to assure the accuracy and staining quality of the slides. - Effectively operate, maintain and troubleshoot all equipment related to tissue preparations and storage. - Recognize, correct, and report problems that would cause inaccurate or misleading results. - Use appropriate handling of hazardous chemicals used for tissue preparation. Requirements Include: - Associates Degree with ASCP or equivalent registry. - Graduate of an accredited Histology Program. - EPIC knowledge preferred. - 1 year of recent paid Histo Tech experience. If you or someone you know is interested in this position contact us today! Erik Dokken, On Assignment Healthcare, erik.dokken@onassignment.com People First -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, December 23, 2010 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 85, Issue 22 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CAP Question regarding procedure manual (WILLIAM DESALVO) 2. Materials to prepare for the registry exam? (vkline2344) 3. if you could pick your own floor surface... (Orr, Rebecca) 4. RE: Materials to prepare for the registry exam? (Sands, Jenn) 5. RE: CAP Question regarding procedure manual (BSullivan@shorememorial.org) 6. RE: if you could pick your own floor surface... (Feher, Stephen) 7. RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject (WILLIAM DESALVO) 8. Re: Used embedding center and microtome (Jay Lundgren) 9. RE: [SPAM-HC] - RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject - Email found in subject (Cazares, Ruth) 10. Parathyroid staining (Webb, Dorothy L) ---------------------------------------------------------------------- Message: 1 Date: Thu, 23 Dec 2010 06:26:40 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] CAP Question regarding procedure manual To: , Victor Tobias Cc: histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Beatrice, How do you show proof to CAP that your employees that use the procedures and perform the tasks described in the procedures have reviewed and understand the procedure when there is a new one during your cycle year and before it is implemented? CAP has changed the way they inspect and they are now looking for how whether the employees performing a task described in the procedure understands the procedure and is performing exactly as the procedure describes. They are not as concerned that the Supervisor and management knows about the procedures, they want to see how informed, trained and competent the bench employee is. William DeSalvo, B.S., HTL(ASCP) > To: victor@pathology.washington.edu > From: BSullivan@shorememorial.org > Date: Thu, 23 Dec 2010 07:53:22 -0500 > Subject: Re: [Histonet] CAP Question regarding procedure manual > CC: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > Victor, > To my knowledge all you need is proof that the staff reviewed the manuals. > We accomplish this by a sign off sheet in the front of each manual we use. > The Supervisor, or designee, needs to review and sign off on each procedure > in each manual. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > Speak only well of people and you need never whisper > > > > Victor Tobias > .washington.edu> To > Sent by: Histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] CAP Question regarding > 12/22/2010 06:16 procedure manual > PM > > > > > > > > > > Is there a requirement to have a signature of every staff member on a > procedure if they perform that procedure in a manual? Wouldn't one > signature on a cover page suffice that you have read and understand what > is in the manual? > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 23 Dec 2010 09:14:01 -0500 From: "vkline2344" Subject: [Histonet] Materials to prepare for the registry exam? To: Message-ID: <11FD5E30DE9042EE94C341A6A2CE9114@vicPC> Content-Type: text/plain; charset="iso-8859-1" I was curious if anyone has ever used the Flashcards based on the Carson book to prepare for the registry exam and if so, if you recommend them or not? I have the Board of Registry Study Guide, but was thinking of additional materials to have on hand for when I start to prepare for the registry exam. I'm currently in school and plan to graduate in June and would like to take my exam soon after. Thanks so much and Happy Holidays! Victoria ------------------------------ Message: 3 Date: Thu, 23 Dec 2010 08:21:18 -0600 From: "Orr, Rebecca" Subject: [Histonet] if you could pick your own floor surface... To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Friends, What type of floor surface would you pick if you could design your own histology lab? I was walking through Costco the other night and noticed theirs is just concrete. Compensating for leg fatigue on the Assistants who have to stand, I'm thinking this type of surface might be easiest to keep clean. What do you think about just concrete? Would there need to be a paraffin scrape nightly? Would that be enough? If paraffin is tracked in the morning will this type of floor be slippy (Pittsburgh slang) by 3pm? What flooring do you have and what drawbacks and advantages do you see with it? Many thanks and no skids! Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ********* Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ------------------------------ Message: 4 Date: Thu, 23 Dec 2010 08:35:32 -0600 From: "Sands, Jenn" Subject: RE: [Histonet] Materials to prepare for the registry exam? To: "vkline2344" , Message-ID: <3F317E4D03ED254183E9FB1840E71F38015CCF55@Exchange01.dakcl.com> Content-Type: text/plain; charset="us-ascii" I used them to take my HTL exam. I found them quite helpful because not only do they give you the correct answer, but they explain why it is correct and why the others are not. Good Luck. Jenn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of vkline2344 Sent: Thursday, December 23, 2010 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Materials to prepare for the registry exam? I was curious if anyone has ever used the Flashcards based on the Carson book to prepare for the registry exam and if so, if you recommend them or not? I have the Board of Registry Study Guide, but was thinking of additional materials to have on hand for when I start to prepare for the registry exam. I'm currently in school and plan to graduate in June and would like to take my exam soon after. Thanks so much and Happy Holidays! Victoria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------ This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. ------------------------------ Message: 5 Date: Thu, 23 Dec 2010 09:55:37 -0500 From: BSullivan@shorememorial.org Subject: RE: [Histonet] CAP Question regarding procedure manual To: WILLIAM DESALVO Cc: histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII When there is a change or new procedure made part of the existing manual it is noted on a separate sheet designated for this. After this new or changed procedure is signed off by the Medical Director it is given to the rest of the department to read. This is initialed by each person on that sheet. These sheets are within the manuals. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper WILLIAM DESALVO To , 12/23/2010 08:26 Victor Tobias AM cc histonet , Subject RE: [Histonet] CAP Question regarding procedure manual Beatrice, How do you show proof to CAP that your employees that use the procedures and perform the tasks described in the procedures have reviewed and understand the procedure when there is a new one during your cycle year and before it is implemented? CAP has changed the way they inspect and they are now looking for how whether the employees performing a task described in the procedure understands the procedure and is performing exactly as the procedure describes. They are not as concerned that the Supervisor and management knows about the procedures, they want to see how informed, trained and competent the bench employee is. William DeSalvo, B.S., HTL(ASCP) > To: victor@pathology.washington.edu > From: BSullivan@shorememorial.org > Date: Thu, 23 Dec 2010 07:53:22 -0500 > Subject: Re: [Histonet] CAP Question regarding procedure manual > CC: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > Victor, > To my knowledge all you need is proof that the staff reviewed the manuals. > We accomplish this by a sign off sheet in the front of each manual we use. > The Supervisor, or designee, needs to review and sign off on each procedure > in each manual. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > Speak only well of people and you need never whisper > > > > Victor Tobias > .washington.edu> To > Sent by: Histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] CAP Question regarding > 12/22/2010 06:16 procedure manual > PM > > > > > > > > > > Is there a requirement to have a signature of every staff member on a > procedure if they perform that procedure in a manual? Wouldn't one > signature on a cover page suffice that you have read and understand what > is in the manual? > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 23 Dec 2010 10:50:22 -0500 From: "Feher, Stephen" Subject: RE: [Histonet] if you could pick your own floor surface... To: "Orr, Rebecca" , Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC2466969F@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" Hi Rebecca, We built a Pathology lab from scratch last year and this was a question that we explored when deciding on flooring materials. We ended up choosing poured epoxy (with rubberized compounds embedded) over concrete. The floor is seamless, wax and slip and stain resistant, and very easy to keep clean. We do a minimum of scrapping a the end of each day. At the end of a year, the floor has stood up extremely well and looks great. It costs a little more up front but you end up saving in the costs of keeping it clean and wax free and in replacement costs. It is estimated that we will not have to look at replacing it for at least 10 years. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Thursday, December 23, 2010 9:21 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] if you could pick your own floor surface... Hi Friends, What type of floor surface would you pick if you could design your own histology lab? I was walking through Costco the other night and noticed theirs is just concrete. Compensating for leg fatigue on the Assistants who have to stand, I'm thinking this type of surface might be easiest to keep clean. What do you think about just concrete? Would there need to be a paraffin scrape nightly? Would that be enough? If paraffin is tracked in the morning will this type of floor be slippy (Pittsburgh slang) by 3pm? What flooring do you have and what drawbacks and advantages do you see with it? Many thanks and no skids! Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ********* Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 23 Dec 2010 09:49:53 -0700 From: WILLIAM DESALVO Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject To: , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Thanks, I am aware of Peggy's form. We have similar hard copy forms now, but we are moving to an electronic system. It will be much more manageable for 125 employees. When you state in your form the employee is competent, do you have documentation to support that there was a competency evaluation? We only state the employee has read and understands. We have separate documentation for training and competency. I love this type of discussion string. There are so many different processes out in Histoland. Do others document separately the original creation, training, competency, implementation of procedures/processes and then continual yearly training, competency and review of those processes? Are you using a Quality Management System? How about Electronic system to manage? William DeSalvo, B.S., HTL(ASCP) > From: RCazares@schosp.org > To: wdesalvo.cac@hotmail.com > Date: Thu, 23 Dec 2010 10:17:48 -0600 > Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > Hello histonetters, > > When I implement a new procedure in my lab, besides the routine yearly sign-off sheet that accompanies the procedure, there an additional sheet that states > > " The following employees have reviewed this procedure and by signing below are acknowledging that they understand and are competent in performing the procedure:" > > Below that statement is a box with signature and date lines for all the employees using this procedure to sign. This makes each employee responsible for reading and understanding the procedure. This I got from one of Peggy Wenk's workshops (Thanks Peggy!!). I can send a copy to anyone who'd like one, just contact me directly. > > > Ruth Cazares, HT (ASCP) > Histology Supervisor > Department of Pathology > Swedish Covenant Hospital > Chicago, IL > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Thursday, December 23, 2010 7:27 AM > To: bsullivan@shorememorial.org; Victor Tobias > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > > Beatrice, > How do you show proof to CAP that your employees that use the procedures and perform the tasks described in the procedures have reviewed and understand the procedure when there is a new one during your cycle year and before it is implemented? > > CAP has changed the way they inspect and they are now looking for how whether the employees performing a task described in the procedure understands the procedure and is performing exactly as the procedure describes. They are not as concerned that the Supervisor and management knows about the procedures, they want to see how informed, trained and competent the bench employee is. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > To: victor@pathology.washington.edu > > From: BSullivan@shorememorial.org > > Date: Thu, 23 Dec 2010 07:53:22 -0500 > > Subject: Re: [Histonet] CAP Question regarding procedure manual > > CC: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > > > Victor, > > To my knowledge all you need is proof that the staff reviewed the manuals. > > We accomplish this by a sign off sheet in the front of each manual we use. > > The Supervisor, or designee, needs to review and sign off on each procedure > > in each manual. > > > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > > AP Supervisor > > Shore Memorial Hospital > > 609-653-3590 > > > > > > Speak only well of people and you need never whisper > > > > > > > > Victor Tobias > > > .washington.edu> To > > Sent by: Histonet > > histonet-bounces@ > > lists.utsouthwest cc > > ern.edu > > Subject > > [Histonet] CAP Question regarding > > 12/22/2010 06:16 procedure manual > > PM > > > > > > > > > > > > > > > > > > > > Is there a requirement to have a signature of every staff member on a > > procedure if they perform that procedure in a manual? Wouldn't one > > signature on a cover page suffice that you have read and understand what > > is in the manual? > > > > Victor > > > > -- > > Victor Tobias > > Clinical Applications Analyst > > University of Washington Medical Center > > Dept of Pathology Room BB220 > > 1959 NE Pacific > > Seattle, WA 98195 > > victor@pathology.washington.edu > > 206-598-2792 > > 206-598-7659 Fax > > ================================================= > > Privileged, confidential or patient identifiable information may be > > contained in this message. This information is meant only for the use > > of the intended recipients. If you are not the intended recipient, or > > if the message has been addressed to you in error, do not read, > > disclose, reproduce, distribute, disseminate or otherwise use this > > transmission. Instead, please notify the sender by reply e-mail, and > > then destroy all copies of the message and any attachments. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > > Thank you for your cooperation. ------------------------------ Message: 8 Date: Thu, 23 Dec 2010 10:59:50 -0600 From: Jay Lundgren Subject: Re: [Histonet] Used embedding center and microtome To: alineumann@aol.com Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 23 Dec 2010 11:04:16 -0600 From: "Cazares, Ruth" Subject: RE: [SPAM-HC] - RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject - Email found in subject To: 'WILLIAM DESALVO' Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <572D1F45B44B3D4096D554B4CB40639C032034E8C1@EXCHCCRMB.schosp.org> Content-Type: text/plain; charset="us-ascii" Hi William, Our competency evaluation is done once a year as part of the employee's annual evaluation. I don't actually have each employee perform the procedure as part of the documentation, although................. hmmmm, maybe an in-service to discuss the new procedure with all employees? I agree with you about discussing things on Histonet, I have found it to be a lifesaver on more than one occasion (OK, so maybe that's a little dramatic, but you get the point) :) Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Thursday, December 23, 2010 10:50 AM To: Cazares, Ruth; histonet Subject: [SPAM-HC] - RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject - Email found in subject Thanks, I am aware of Peggy's form. We have similar hard copy forms now, but we are moving to an electronic system. It will be much more manageable for 125 employees. When you state in your form the employee is competent, do you have documentation to support that there was a competency evaluation? We only state the employee has read and understands. We have separate documentation for training and competency. I love this type of discussion string. There are so many different processes out in Histoland. Do others document separately the original creation, training, competency, implementation of procedures/processes and then continual yearly training, competency and review of those processes? Are you using a Quality Management System? How about Electronic system to manage? William DeSalvo, B.S., HTL(ASCP) > From: RCazares@schosp.org > To: wdesalvo.cac@hotmail.com > Date: Thu, 23 Dec 2010 10:17:48 -0600 > Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > Hello histonetters, > > When I implement a new procedure in my lab, besides the routine yearly sign-off sheet that accompanies the procedure, there an additional sheet that states > > " The following employees have reviewed this procedure and by signing below are acknowledging that they understand and are competent in performing the procedure:" > > Below that statement is a box with signature and date lines for all the employees using this procedure to sign. This makes each employee responsible for reading and understanding the procedure. This I got from one of Peggy Wenk's workshops (Thanks Peggy!!). I can send a copy to anyone who'd like one, just contact me directly. > > > Ruth Cazares, HT (ASCP) > Histology Supervisor > Department of Pathology > Swedish Covenant Hospital > Chicago, IL > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Thursday, December 23, 2010 7:27 AM > To: bsullivan@shorememorial.org; Victor Tobias > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > > Beatrice, > How do you show proof to CAP that your employees that use the procedures and perform the tasks described in the procedures have reviewed and understand the procedure when there is a new one during your cycle year and before it is implemented? > > CAP has changed the way they inspect and they are now looking for how whether the employees performing a task described in the procedure understands the procedure and is performing exactly as the procedure describes. They are not as concerned that the Supervisor and management knows about the procedures, they want to see how informed, trained and competent the bench employee is. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > To: victor@pathology.washington.edu > > From: BSullivan@shorememorial.org > > Date: Thu, 23 Dec 2010 07:53:22 -0500 > > Subject: Re: [Histonet] CAP Question regarding procedure manual > > CC: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > > > Victor, > > To my knowledge all you need is proof that the staff reviewed the manuals. > > We accomplish this by a sign off sheet in the front of each manual we use. > > The Supervisor, or designee, needs to review and sign off on each procedure > > in each manual. > > > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > > AP Supervisor > > Shore Memorial Hospital > > 609-653-3590 > > > > > > Speak only well of people and you need never whisper > > > > > > > > Victor Tobias > > > .washington.edu> To > > Sent by: Histonet > > histonet-bounces@ > > lists.utsouthwest cc > > ern.edu > > Subject > > [Histonet] CAP Question regarding > > 12/22/2010 06:16 procedure manual > > PM > > > > > > > > > > > > > > > > > > > > Is there a requirement to have a signature of every staff member on a > > procedure if they perform that procedure in a manual? Wouldn't one > > signature on a cover page suffice that you have read and understand what > > is in the manual? > > > > Victor > > > > -- > > Victor Tobias > > Clinical Applications Analyst > > University of Washington Medical Center > > Dept of Pathology Room BB220 > > 1959 NE Pacific > > Seattle, WA 98195 > > victor@pathology.washington.edu > > 206-598-2792 > > 206-598-7659 Fax > > ================================================= > > Privileged, confidential or patient identifiable information may be > > contained in this message. This information is meant only for the use > > of the intended recipients. If you are not the intended recipient, or > > if the message has been addressed to you in error, do not read, > > disclose, reproduce, distribute, disseminate or otherwise use this > > transmission. Instead, please notify the sender by reply e-mail, and > > then destroy all copies of the message and any attachments. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > > Thank you for your cooperation. ________________________________ *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. ------------------------------ Message: 10 Date: Thu, 23 Dec 2010 11:08:27 -0600 From: "Webb, Dorothy L" Subject: [Histonet] Parathyroid staining To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43EA95058346@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Does anyone use Toluidine blue to stain parathyroids when they come in fresh for a frozen section? One of our pathologist heard of this and I am not familiar with it. I have heard of it with the Paragon stain, but not certain what percentage would be needed if only Toluidine blue was used. Thanks ahead of time and Happy Holidays!! Dorothy Webb, HT ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 85, Issue 22 **************************************** From MadaryJ <@t> MedImmune.com Thu Dec 23 12:34:31 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Thu Dec 23 12:34:34 2010 Subject: [Histonet] Flash Card studying Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1302F88712@MD1EV002.medimmune.com> I am all for any study style that will help you recall for the test. Given the HT test in 81 and the massive change in 2000 for the HTL, I think it is best to just absolutely know the material by say reading the suggested texts 2 or 3 times. I did that and it really worked. I read a chapter a night and went through several books, several times and it really helped me with the exam as well as in the lab. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From kgalletti <@t> pekinhospital.com Thu Dec 23 12:35:49 2010 From: kgalletti <@t> pekinhospital.com (Kela D. Galletti) Date: Thu Dec 23 12:35:58 2010 Subject: [Histonet] RE: if you could pick your own floor surface... In-Reply-To: References: Message-ID: <96C498EC78677A4FB263998E5B1EDA212484B54B0D@exchange1.pekinhospital.com> If you work with picric acid (or Bouin's) in the lab, don't go with concrete. Picric acid reacts strongly on contact with concrete. Weird, huh? Kela Galletti Histology Supervisor Pekin Hospital -----Original Message----- Message: 3 Date: Thu, 23 Dec 2010 08:21:18 -0600 From: "Orr, Rebecca" Subject: [Histonet] if you could pick your own floor surface... To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Friends, What type of floor surface would you pick if you could design your own histology lab? I was walking through Costco the other night and noticed theirs is just concrete. Compensating for leg fatigue on the Assistants who have to stand, I'm thinking this type of surface might be easiest to keep clean. What do you think about just concrete? Would there need to be a paraffin scrape nightly? Would that be enough? If paraffin is tracked in the morning will this type of floor be slippy (Pittsburgh slang) by 3pm? What flooring do you have and what drawbacks and advantages do you see with it? Many thanks and no skids! Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ********* Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. From rsrichmond <@t> gmail.com Thu Dec 23 12:50:48 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Dec 23 12:50:57 2010 Subject: [Histonet] Re: if you could pick your own floor surface... Message-ID: Steve Feher responds >>We built a pathology lab from scratch last year and this was a question that we explored when deciding on flooring materials. We ended up choosing poured epoxy (with rubberized compounds embedded) over concrete. The floor is seamless, wax and slip and stain resistant, and very easy to keep clean. We do a minimum of scraping a the end of each day. At the end of a year, the floor has stood up extremely well and looks great. It costs a little more up front but you end up saving in the costs of keeping it clean and wax free and in replacement costs. It is estimated that we will not have to look at replacing it for at least 10 years.<< Steve, could you give us some information about where you actually get that poured epoxy material? While talking about pathology floors - do remember indoor-outdoor carpet for the pathologist's office. It helps so much to keep slides from breaking when I drop them! Bob Richmond Samurai Pathologist Knoxville TN From rsrichmond <@t> gmail.com Thu Dec 23 12:58:45 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Dec 23 12:58:51 2010 Subject: [Histonet] Re: Parathyroid stain Message-ID: Dorothy Webb asks: >>Does anyone use toluidine blue to stain parathyroids when they come in fresh for a frozen section? One of our pathologists heard of this and I am not familiar with it. I have heard of it with the Paragon stain, but not certain what percentage would be needed if only toluidine blue was used.<< I'm not sure why anyone would want to do this. You could probably use Diff-Quik II (or a generic equivalent) instead of mixing your own toluidine blue. If anyone wants the formula for the old Paragon stain for frozen sections it's in our archives at: http://www.histosearch.com/histonet/Feb00/Re.wantedifpossibleParagoA.html Bob Richmond Samurai Pathologist Knoxville TN From sfeher <@t> CMC-NH.ORG Thu Dec 23 13:02:39 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Dec 23 13:02:45 2010 Subject: [Histonet] Re: if you could pick your own floor surface... In-Reply-To: References: Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC246696A9@exchange.cmc-nh.org> We contracted with a flooring company and described what we wanted to do. I will find out exactly what they used. It is similar to the poured epoxy that one would use on a garage floor but with the addition of an additional material that actually has some give to it when walked upon. I will post the exact materials as soon as I get them. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, December 23, 2010 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: if you could pick your own floor surface... Steve Feher responds >>We built a pathology lab from scratch last year and this was a >>question that we explored when deciding on flooring materials. We ended up choosing poured epoxy (with rubberized compounds embedded) over concrete. The floor is seamless, wax and slip and stain resistant, and very easy to keep clean. We do a minimum of scraping a the end of each day. At the end of a year, the floor has stood up extremely well and looks great. It costs a little more up front but you end up saving in the costs of keeping it clean and wax free and in replacement costs. It is estimated that we will not have to look at replacing it for at least 10 years.<< Steve, could you give us some information about where you actually get that poured epoxy material? While talking about pathology floors - do remember indoor-outdoor carpet for the pathologist's office. It helps so much to keep slides from breaking when I drop them! Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtaylor <@t> meriter.com Thu Dec 23 13:13:33 2010 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu Dec 23 13:13:38 2010 Subject: [Histonet] MPB antibody Message-ID: Hi Everyone, Has anyone worked with the MBP (human eosinophilic major basic protein) antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From BBranton <@t> sarapath.com Thu Dec 23 13:30:17 2010 From: BBranton <@t> sarapath.com (Brian Branton) Date: Thu Dec 23 13:38:36 2010 Subject: [Histonet] Please disable my email account Message-ID: Histonet, Please disable my account. I will be out of the office the week of December 27 to the 31st. I don't want my "Out of Office Assistant" sending extra emails to the histonet. Thanks, Brian Branton Purchasing Agent SaraPath Diagnostics Sarasota Pathology Sarasota Professional Enterprises II (941) 362-8963 (941) 362-8964 FAX From MSHERWOOD <@t> PARTNERS.ORG Thu Dec 23 14:12:10 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Dec 23 14:12:15 2010 Subject: [Histonet] Please disable my email account In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB531C@PHSXMB30.partners.org> You do it yourself by simply unsubscribing. Follow the instructions on Histonet.org. Then when you return, you subscribe again. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brian Branton Sent: Thursday, December 23, 2010 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Please disable my email account Histonet, Please disable my account. I will be out of the office the week of December 27 to the 31st. I don't want my "Out of Office Assistant" sending extra emails to the histonet. Thanks, Brian Branton Purchasing Agent SaraPath Diagnostics Sarasota Pathology Sarasota Professional Enterprises II (941) 362-8963 (941) 362-8964 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From pbaldwin <@t> micronenvironmental.com Thu Dec 23 14:28:28 2010 From: pbaldwin <@t> micronenvironmental.com (pbaldwin@MicronEnvironmental.com) Date: Thu Dec 23 14:28:45 2010 Subject: [Histonet] Green Chemistry Blog Message-ID: <20101223122828.ED8D42AE@resin09.mta.everyone.net> As a long-time Histonet reader but an infrequent poster, I thought that some of you may be interested in our new blog on "green" work practices for healthcare laboratories We will NOT promote our firm or products, but WILL promote environmentally safe procedures that healthcare laboratories can follow to reduce a universe of hazards. Our first posting suggests how labs can conduct a "self-audit" to compare their use of hazardous and nonhazaordous chemicals ([1]http://greenchemistryforlife.wordpress.com). I hope that the topic of "green chemistry" can be of value to some of you and I'd welcoome your feedback. Thank you for your interest - Peter Peter G. Baldwin Director of Operations, Marketing & Sales pbaldwin@MicronEnvironmental.com Micron Environmental Industries, Inc. Green Chemistry for Life www.MicronEnvironmental.com 1221 Cameron Street Alexandria, VA 22314 703-548-2776 703-548-7988/Fax References 1. http://greenchemistryforlife.wordpress.com/ From AnthonyH <@t> chw.edu.au Thu Dec 23 15:56:34 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Dec 23 15:56:51 2010 Subject: [Histonet] RE: Parathyroid staining In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43EA95058346@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43EA95058346@HPEMX3.HealthPartners.int> Message-ID: <6D6BD1DE8A5571489398B392A38A7157032280@xmdb02.nch.kids> Dorothy, A 0.5% aqueous Toluidine blue will suffice. Stain either air-dried or alcohol fixed frozen sections for 1-2 minutes, rinse in water, dehydrate quickly, clear and mount. That's it! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, 24 December 2010 4:08 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Parathyroid staining Does anyone use Toluidine blue to stain parathyroids when they come in fresh for a frozen section? One of our pathologist heard of this and I am not familiar with it. I have heard of it with the Paragon stain, but not certain what percentage would be needed if only Toluidine blue was used. Thanks ahead of time and Happy Holidays!! Dorothy Webb, HT ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From w_alkadhumi <@t> yahoo.com Fri Dec 24 10:30:29 2010 From: w_alkadhumi <@t> yahoo.com (wassan alkadhumi) Date: Fri Dec 24 10:30:34 2010 Subject: [Histonet] IHC theoretical question Message-ID: <319989.62627.qm@web45214.mail.sp1.yahoo.com> Dear histonet members I have a?theoretical?question concerning IHC, we do HRP method using DAKO materials, first step in?immuon staining is to add peroxidase blocking solution to quench endogenus peroxidase. The second step is what am having problem with, we prepare solution from human serum diluted in wash buffer 1:2, as my understanding to why we do this step is to block unwanted proteins in the tissue so that we prevent background staining, but this step is done?before adding primary antibody, wont this step block the antigen too since the antigen is?a protein too? the other theory i hired is the proteins tend to coagulate together?so we add this solution to?dispense them, primary antibody then?can easily attach to the antigen.? we have two histotext books in the hospital and i red the IHC sections,?their was really nothing clear about?why we do?this step and i searched the Internet with no satisfactory answer. As u can see i need help! step #3 primary Ab step#4 link system(secondary Ab against primary Ab (protein) Step #5 chromogen DAB Am doing IHC for a year?and 8 months, till now am confused about this step and when i train people am not sure what to say to them about it. help me please Have a great holy day Wassan Histotechnician Shorsh hospital North of Iraq From anonwums1 <@t> gmail.com Fri Dec 24 13:21:01 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Fri Dec 24 13:21:09 2010 Subject: [Histonet] IHC theoretical question In-Reply-To: <319989.62627.qm@web45214.mail.sp1.yahoo.com> References: <319989.62627.qm@web45214.mail.sp1.yahoo.com> Message-ID: Hi, I find this confusing myself sometimes, so I'll try my best to explain this. I stain mouse tissues. Let's say that I want to stain for the classic macrophage F4/80 marker, so I go buy a rat anti-mouse F4/80. In order to further amplify, I buy a biotinylated donkey anti-rat antibody, and then detect that with HRP/DAB. Certain parts of the section may be sticky and nonspecifically bind proteins or even have an affinity for antibodies. If I didn't block, my primary and secondary antibodies might just bind to them, adding a lot of background. Pre-incubating with protein such as serum, BSA, or milk will bind to those and prevent nonspecific binding of your primary or secondary antibodies. Serum is typically used because it contains antibodies, so you would also saturate anything that is nonspecifically but preferentially binding antibodies such as Fc receptors (although sometimes you have add Fc block if this is particularly problematic). Typically serum from the secondary antibody host (in this case, donkey) is used. Let's say your tissue has sticky parts and instead of blocking with donkey serum as you should, you accidentally block with rat serum. Now your entire section is coated with low levels of rat IgG that was in your rat serum, so when you add your anti-rat secondary antibody, it will bind everywhere. If you block with rabbit serum, there still is a chance that your anti-rat secondary may cross react with the rabbit IgG. Now if you use donkey serum, there is pretty much no chance that the donkey raised antibodies against donkey IgG unless the donkey had some bizarre autoimmune disease. So that's why you typically add serum derived from the secondary antibody host. Hopefully, your antigen doesn't nonspecifically bind stuff, or else IHC is going to be very hard. Most antigens don't nonspecifically bind stuff, so you're good. Is that clear? Adam On Fri, Dec 24, 2010 at 10:30 AM, wassan alkadhumi wrote: > Dear histonet members > I have a theoretical question concerning IHC, we do HRP method using DAKO > materials, first step in immuon staining is to add peroxidase blocking > solution > to quench endogenus peroxidase. The second step is what am having problem > with, > we prepare solution from human serum diluted in wash buffer 1:2, as my > understanding to why we do this step is to block unwanted proteins in the > tissue > so that we prevent background staining, but this step is done before adding > primary antibody, wont this step block the antigen too since the antigen > is a > protein too? > the other theory i hired is the proteins tend to coagulate together so we > add > this solution to dispense them, primary antibody then can easily attach to > the > antigen. > we have two histotext books in the hospital and i red the IHC > sections, their > was really nothing clear about why we do this step and i searched the > Internet > with no satisfactory answer. > As u can see i need help! > step #3 primary Ab > step#4 link system(secondary Ab against primary Ab (protein) > Step #5 chromogen DAB > Am doing IHC for a year and 8 months, till now am confused about this step > and > when i train people am not sure what to say to them about it. > help me please > Have a great holy day > > Wassan > Histotechnician > Shorsh hospital > North of Iraq > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From anonwums1 <@t> gmail.com Fri Dec 24 13:29:01 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Fri Dec 24 13:29:07 2010 Subject: [Histonet] Hazy background during IF Message-ID: Seasons Greeting from the laboratory: I've been having a somewhat puzzling issue with my IFs lately. I stain mostly fixed, decalcified mouse bones, and recently, my sections have this strange haze over them. It's not consistently but it does crop up in maybe 10% of sections. It looks like a dense fog settled over my sections, especially in the red/594/TxRed channel, often obscuring the signal. It sometimes does this it the green channel and rarely in the DAPI channel. If I carefully strip off the coverslip and soak the sections in PBS for a few minutes, and then re-mount, the problem goes away. I'm guessing it's a problem in the mounting somewhere. Here is my protocol: Sections are circled with a PAP pen. All liquids are dispensed using a 200 uL pipetter onto it Finish staining Wash 3 x 5' in TBS-T Add a drop of Prolong Gold Antifade (I previously used Vectashield and had the same problem) Put on coverslip Dab corners of coverslip with clear nailpolish Let sit overnight for mounting media to cure Any ideas what could be going on? Thanks, Adam From gu.lang <@t> gmx.at Fri Dec 24 13:35:42 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Dec 24 13:35:47 2010 Subject: AW: [Histonet] IHC theoretical question In-Reply-To: <319989.62627.qm@web45214.mail.sp1.yahoo.com> References: <319989.62627.qm@web45214.mail.sp1.yahoo.com> Message-ID: The normal blocking serum should be from the species of your secondary antibody. The unspecific binding sites are occupied by the proteins (eg. electrostatic binding), so the primary antibody can't attach. But the specific antigen-antibody reaction should not be affected. So this results in primary antibody on your specific epitops and "secondary" proteins covering the rest of negativ-charged tissue elements. The secondary antibody binds to the primary-fc fragment and doesn't mind other proteins of it's own species. If you use commercial antibody-dilution-solution, the normal-serum-step can be omitted, because they usually content BSA to block unspecific bindings. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von wassan alkadhumi Gesendet: Freitag, 24. Dezember 2010 17:30 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] IHC theoretical question Dear histonet members I have a?theoretical?question concerning IHC, we do HRP method using DAKO materials, first step in?immuon staining is to add peroxidase blocking solution to quench endogenus peroxidase. The second step is what am having problem with, we prepare solution from human serum diluted in wash buffer 1:2, as my understanding to why we do this step is to block unwanted proteins in the tissue so that we prevent background staining, but this step is done?before adding primary antibody, wont this step block the antigen too since the antigen is?a protein too? the other theory i hired is the proteins tend to coagulate together?so we add this solution to?dispense them, primary antibody then?can easily attach to the antigen.? we have two histotext books in the hospital and i red the IHC sections,?their was really nothing clear about?why we do?this step and i searched the Internet with no satisfactory answer. As u can see i need help! step #3 primary Ab step#4 link system(secondary Ab against primary Ab (protein) Step #5 chromogen DAB Am doing IHC for a year?and 8 months, till now am confused about this step and when i train people am not sure what to say to them about it. help me please Have a great holy day Wassan Histotechnician Shorsh hospital North of Iraq _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Fri Dec 24 18:44:36 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Dec 24 18:44:42 2010 Subject: [Histonet] Re: if you could pick your own floor surface... In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC246696A9@exchange.cmc-nh.org> References: <0908FC0A43B87A4FB60EDCCA06AABC246696A9@exchange.cmc-nh.org> Message-ID: shag carpeting. and it would extend to the walls, up to the ceiling. Writer Richard Suflet recommended drinking large doses of strong vinegar with fleas to cure the illnesses that resulted from swallowing the horse-leeches that were common in drinking water. -Every Home a Distillery, Sarah Meacham On Thu, Dec 23, 2010 at 2:02 PM, Feher, Stephen wrote: > We contracted with a flooring company and described what we wanted to > do. I will find out exactly what they used. It is similar to the > poured epoxy that one would use on a garage floor but with the addition > of an additional material that actually has some give to it when walked > upon. > > I will post the exact materials as soon as I get them. > > > Steve > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert > Richmond > Sent: Thursday, December 23, 2010 1:51 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: if you could pick your own floor surface... > > Steve Feher responds > > >>We built a pathology lab from scratch last year and this was a > >>question > that we explored when deciding on flooring materials. We ended up > choosing poured epoxy (with rubberized compounds embedded) over > concrete. The floor is seamless, wax and slip and stain resistant, and > very easy to keep clean. We do a minimum of scraping a the end of each > day. At the end of a year, the floor has stood up extremely well and > looks great. It costs a little more up front but you end up saving in > the costs of keeping it clean and wax free and in replacement costs. It > is estimated that we will not have to look at replacing it for at least > 10 years.<< > > Steve, could you give us some information about where you actually get > that poured epoxy material? > > While talking about pathology floors - do remember indoor-outdoor carpet > for the pathologist's office. It helps so much to keep slides from > breaking when I drop them! > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Sun Dec 26 19:12:27 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Sun Dec 26 19:12:46 2010 Subject: [Histonet] Chuck Churukian Message-ID: <034BC67A-78AE-4299-9BAC-D4922BF1A891@yahoo.com> I just heard from Irene Churukian today. Please put Chuck in your thoughts and prayers. Irene said this: CLL cells changed in character so Chuck began chemo treatments on Dec. 1. His second one was Dec. 21... He has six altogether - three weeks apart. The first cycle was difficult... he was very weak.... in bed most of the time. He is now improving ... thankfully. He has never been sick and confined to bed/home so he gets cabin fever easily. People have been so kind to visit him. So our Christmas this year was very different from years past. We are hoping and praying that by April, he will be in remission after all the treatments are completed. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From Janice.Mahoney <@t> alegent.org Mon Dec 27 07:34:19 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Mon Dec 27 07:34:30 2010 Subject: [Histonet] Chuck Churukian In-Reply-To: <034BC67A-78AE-4299-9BAC-D4922BF1A891@yahoo.com> References: <034BC67A-78AE-4299-9BAC-D4922BF1A891@yahoo.com> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354FB4@EXCHMBC2.ad.ah.local> So sorry to hear this news. Could someone please provide a home address for Chuck so that we can send get well cards? I would think he could get hundreds! Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Sunday, December 26, 2010 7:12 PM To: Histonet Subject: [Histonet] Chuck Churukian I just heard from Irene Churukian today. Please put Chuck in your thoughts and prayers. Irene said this: CLL cells changed in character so Chuck began chemo treatments on Dec. 1. His second one was Dec. 21... He has six altogether - three weeks apart. The first cycle was difficult... he was very weak.... in bed most of the time. He is now improving ... thankfully. He has never been sick and confined to bed/home so he gets cabin fever easily. People have been so kind to visit him. So our Christmas this year was very different from years past. We are hoping and praying that by April, he will be in remission after all the treatments are completed. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From AHutton <@t> dh.org Mon Dec 27 11:50:45 2010 From: AHutton <@t> dh.org (Hutton, Allison) Date: Mon Dec 27 11:52:49 2010 Subject: [Histonet] job opening in PA Message-ID: <38A56C4F4630D348A50B3720409270870E0FE292@dhmail.dhorg.org> There is an open position for a full time day shift (variable start times) histology technician at Doylestown Hospital in Doylestown, PA. Position is responsible for all aspects of the histology department including, but not limited to, embedding, sectioning, staining (specials and IHC are manual), accessioning, and some clerical duties. We are a community hospital with volumes of approximately 10,500 cases per year with about half of the workload consisting of GI specimens. HT(ASCP) or eligible. Experience preferred. To apply: www.dh.org Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital 595 W. State St Doylestown, PA 18901 215-345-2264 ahutton@dh.org From Kim.Donadio <@t> bhcpns.org Mon Dec 27 14:28:24 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Mon Dec 27 14:28:54 2010 Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject In-Reply-To: Message-ID: Do others document separately the original creation, training, competency, implementation of procedures/processes and then continual yearly training, competency and review of those processes? Are you using a Quality Management System? How about Electronic system to manage? Yes, to all of those. We document original training and then all competencies are part of their yearly eval. We also have the QMS electronic policy/procedure Data base that sends each individual reminders when they need to read a update or sign off on the yearly requirement that they have read and understand the material. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 WILLIAM DESALVO Sent by: histonet-bounces@lists.utsouthwestern.edu 12/23/2010 10:49 AM To , histonet cc Subject RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject Thanks, I am aware of Peggy's form. We have similar hard copy forms now, but we are moving to an electronic system. It will be much more manageable for 125 employees. When you state in your form the employee is competent, do you have documentation to support that there was a competency evaluation? We only state the employee has read and understands. We have separate documentation for training and competency. I love this type of discussion string. There are so many different processes out in Histoland. Do others document separately the original creation, training, competency, implementation of procedures/processes and then continual yearly training, competency and review of those processes? Are you using a Quality Management System? How about Electronic system to manage? William DeSalvo, B.S., HTL(ASCP) > From: RCazares@schosp.org > To: wdesalvo.cac@hotmail.com > Date: Thu, 23 Dec 2010 10:17:48 -0600 > Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > Hello histonetters, > > When I implement a new procedure in my lab, besides the routine yearly sign-off sheet that accompanies the procedure, there an additional sheet that states > > " The following employees have reviewed this procedure and by signing below are acknowledging that they understand and are competent in performing the procedure:" > > Below that statement is a box with signature and date lines for all the employees using this procedure to sign. This makes each employee responsible for reading and understanding the procedure. This I got from one of Peggy Wenk's workshops (Thanks Peggy!!). I can send a copy to anyone who'd like one, just contact me directly. > > > Ruth Cazares, HT (ASCP) > Histology Supervisor > Department of Pathology > Swedish Covenant Hospital > Chicago, IL > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Thursday, December 23, 2010 7:27 AM > To: bsullivan@shorememorial.org; Victor Tobias > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > > Beatrice, > How do you show proof to CAP that your employees that use the procedures and perform the tasks described in the procedures have reviewed and understand the procedure when there is a new one during your cycle year and before it is implemented? > > CAP has changed the way they inspect and they are now looking for how whether the employees performing a task described in the procedure understands the procedure and is performing exactly as the procedure describes. They are not as concerned that the Supervisor and management knows about the procedures, they want to see how informed, trained and competent the bench employee is. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > To: victor@pathology.washington.edu > > From: BSullivan@shorememorial.org > > Date: Thu, 23 Dec 2010 07:53:22 -0500 > > Subject: Re: [Histonet] CAP Question regarding procedure manual > > CC: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > > > Victor, > > To my knowledge all you need is proof that the staff reviewed the manuals. > > We accomplish this by a sign off sheet in the front of each manual we use. > > The Supervisor, or designee, needs to review and sign off on each procedure > > in each manual. > > > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > > AP Supervisor > > Shore Memorial Hospital > > 609-653-3590 > > > > > > Speak only well of people and you need never whisper > > > > > > > > Victor Tobias > > > .washington.edu> To > > Sent by: Histonet > > histonet-bounces@ > > lists.utsouthwest cc > > ern.edu > > Subject > > [Histonet] CAP Question regarding > > 12/22/2010 06:16 procedure manual > > PM > > > > > > > > > > > > > > > > > > > > Is there a requirement to have a signature of every staff member on a > > procedure if they perform that procedure in a manual? Wouldn't one > > signature on a cover page suffice that you have read and understand what > > is in the manual? > > > > Victor > > > > -- > > Victor Tobias > > Clinical Applications Analyst > > University of Washington Medical Center > > Dept of Pathology Room BB220 > > 1959 NE Pacific > > Seattle, WA 98195 > > victor@pathology.washington.edu > > 206-598-2792 > > 206-598-7659 Fax > > ================================================= > > Privileged, confidential or patient identifiable information may be > > contained in this message. This information is meant only for the use > > of the intended recipients. If you are not the intended recipient, or > > if the message has been addressed to you in error, do not read, > > disclose, reproduce, distribute, disseminate or otherwise use this > > transmission. Instead, please notify the sender by reply e-mail, and > > then destroy all copies of the message and any attachments. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > > Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From akemiat3377 <@t> yahoo.com Mon Dec 27 14:57:31 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Mon Dec 27 14:57:36 2010 Subject: [Histonet] Chuck Churukian's Address Message-ID: <563441.88990.qm@web113805.mail.gq1.yahoo.com> Hi All, I just spoke with Chuck a few minutes ago and his spirits are up.? He?is working with a physical therapist this afternoon.? He gave me permission to post his address, and would love to hear from those of you who would like to send him cards.? I think it would really cheer him up.? His address is: 34 Gailhaven Court Rochester, NY 14618 ?Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com From Sharon.Davis-Devine <@t> carle.com Mon Dec 27 14:58:20 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Mon Dec 27 14:58:26 2010 Subject: [Histonet] Use of ammonia water Message-ID: Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From HParker <@t> Skaggs.Net Mon Dec 27 15:15:44 2010 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Mon Dec 27 15:15:13 2010 Subject: [Histonet] What New Tissue Processor Should we get ? In-Reply-To: References: Message-ID: <930EB2E8DF68C544873EDD2A3D5F506004700E6898@email1.skaggs.net> Hi All, If given the choice- what would be the best Tissue Processor to get for a small Path Lab. We have always had VIPs. Ours is about 16 y/o and we need to replace it and use it as a back up or for small biopsies. I was looking at the new VIPs and our medical repair people are very happy w/ the VIPs in the past. I am looking at VIP 5 and 6. Do not need anything too fancy. Just want a workhorse that has little or no issues and is user friendly. Any input is much appreciate on these or other products - private emails great ! Thanks, Helayne Parker hparker@skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. From flnails <@t> texaschildrens.org Mon Dec 27 15:19:37 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon Dec 27 15:19:51 2010 Subject: [Histonet] RE: What New Tissue Processor Should we get ? In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F506004700E6898@email1.skaggs.net> References: <930EB2E8DF68C544873EDD2A3D5F506004700E6898@email1.skaggs.net> Message-ID: As have you, I have also had great service from my VIP's and plan on purchasing the VIP 6. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Monday, December 27, 2010 3:16 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] What New Tissue Processor Should we get ? Hi All, If given the choice- what would be the best Tissue Processor to get for a small Path Lab. We have always had VIPs. Ours is about 16 y/o and we need to replace it and use it as a back up or for small biopsies. I was looking at the new VIPs and our medical repair people are very happy w/ the VIPs in the past. I am looking at VIP 5 and 6. Do not need anything too fancy. Just want a workhorse that has little or no issues and is user friendly. Any input is much appreciate on these or other products - private emails great ! Thanks, Helayne Parker hparker@skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From mnglegal <@t> gmail.com Tue Dec 28 00:04:46 2010 From: mnglegal <@t> gmail.com (M.N.) Date: Tue Dec 28 00:05:00 2010 Subject: [Histonet] Listing Message-ID: Hi - I am looking for someone in the LA area (ideally someone who works in a lab or hospital setting) to teach me how to process biopsies from start to finish, and to cut paraffin-embedded sections. I would compensate you for your time, of course. Please contact me with your availability - I'd like to get started soon. Thanks! From Susan.Walzer <@t> HCAHealthcare.com Tue Dec 28 01:57:56 2010 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Dec 28 01:58:03 2010 Subject: [Histonet] RE: Use of ammonia water In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2B3D09D7CA@FWDCWPMSGCMS09.hca.corpad.net> We have always used water with liquid soap and some ammonia to soften blocks. It has had no effect on IHC. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Monday, December 27, 2010 3:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Use of ammonia water Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Dec 28 04:51:55 2010 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Dec 28 04:52:12 2010 Subject: [Histonet] Use of ammonia water In-Reply-To: References: Message-ID: I use it on particularly difficult blocks and have seen no ill effects. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Monday, December 27, 2010 3:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Use of ammonia water Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Tue Dec 28 08:40:19 2010 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Tue Dec 28 08:40:30 2010 Subject: [Histonet] RE: MPB antibody In-Reply-To: References: Message-ID: Hi Jean, I use Leica's MBP which the get from NovoCastra cat. NCL-MBP I use it on formalin fixed paraffin embedded human tissue on my Dako. I use Dako's Target Ret. Soln in a steamer for 40 minutes Incubate 15 min with it at the dilution of 1:25. Then I use Dako LSAB2 kit. It works fine. Good luck, Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Thursday, December 23, 2010 2:14 PM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] MPB antibody Hi Everyone, Has anyone worked with the MBP (human eosinophilic major basic protein) antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From w_alkadhumi <@t> yahoo.com Tue Dec 28 08:49:54 2010 From: w_alkadhumi <@t> yahoo.com (wassan alkadhumi) Date: Tue Dec 28 08:49:58 2010 Subject: [Histonet] bouin solution Message-ID: <604286.3701.qm@web45206.mail.sp1.yahoo.com> Dear Histonet I have question concerning?bouin solution, we use it in masson trichrome before starting the stain.?we incubate the slides trimmed at 3 micron in bouin solution for 18 hr in room temp which is not controlled.?Then?we wash the slides?using running water first and?then distilled water, but?according to what i?red,?when?using bouin solution,the tissue?should?be washed with (50-70)% alcohol?to prevent deterioration of staining in time. now i should mention?that in the?our ?masson trichrome?procedure?there is no washing with alcohol?but only with water. what should i do? In the book its mentioned that we have to wash with alcohol if the tissue is not processed yet, is that why the alcohol step is?missing in masson tri procedure?and why will the stain deteriorate if some of the bouin solution stayed in the tissue? The?main fixative that is used in our lab for all grooses and biopsy is 10% formalin (except bone marrow biopsy). bouin solution is used after processing of the tissue and deparaffinization of the sections is finished. Thanks Wassan Histotechnician Shorh hospital North of Iraq From gu.lang <@t> gmx.at Tue Dec 28 10:42:52 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Dec 28 10:43:00 2010 Subject: AW: [Histonet] bouin solution In-Reply-To: <604286.3701.qm@web45206.mail.sp1.yahoo.com> References: <604286.3701.qm@web45206.mail.sp1.yahoo.com> Message-ID: <2CFC6E180EFB469AA061237DC32D690B@dielangs.at> We incubate the trichrome-slides after deparaffination for 2 hours in 56?C, then rinse in warm tapwater until the slides are colourless. Then another rinse in distilled water, because I don't want carry over from tapwater into the weigert's iron hematoxylin. ... If you let bouins in the tissue, it acts like a stain. Think of vanGieson staining solution (also called picrofuchsin). It sounds like the processing after bouin-fixation and the trichrom staining are mixed up. Or the protocols of trichrom-staining of NBF-fixed and bouin-fixed tissue are mixed up. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von wassan alkadhumi Gesendet: Dienstag, 28. Dezember 2010 15:50 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] bouin solution Dear Histonet I have question concerning?bouin solution, we use it in masson trichrome before starting the stain.?we incubate the slides trimmed at 3 micron in bouin solution for 18 hr in room temp which is not controlled.?Then?we wash the slides?using running water first and?then distilled water, but?according to what i?red,?when?using bouin solution,the tissue?should?be washed with (50-70)% alcohol?to prevent deterioration of staining in time. now i should mention?that in the?our ?masson trichrome?procedure?there is no washing with alcohol?but only with water. what should i do? In the book its mentioned that we have to wash with alcohol if the tissue is not processed yet, is that why the alcohol step is?missing in masson tri procedure?and why will the stain deteriorate if some of the bouin solution stayed in the tissue? The?main fixative that is used in our lab for all grooses and biopsy is 10% formalin (except bone marrow biopsy). bouin solution is used after processing of the tissue and deparaffinization of the sections is finished. Thanks Wassan Histotechnician Shorh hospital North of Iraq _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Tue Dec 28 11:47:13 2010 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Dec 28 11:47:18 2010 Subject: [Histonet] Use of ammonia water In-Reply-To: References: Message-ID: Hi Sharon, I use a 1.5% solution for bone marrow cases and bloody tissue. It does also work well for GI. I've never had an issue with it affecting any of my IHC. An alternative though (when I can't get my hands on ammonium hydroxide) is to put these blocks in warm water (I'm careful about the waterbath because of X-contamination issues) for about 30-45 seconds and this works just as well. I stopped using any surface decal solution or Nair as I did see some differences with the expression of certain markers - I did not see any false negs, but the staining itself was not as intense it looked like. Vikki On Mon, Dec 27, 2010 at 3:58 PM, Sharon.Davis-Devine < Sharon.Davis-Devine@carle.com> wrote: > Happy Holidays Histonetters, I have a question for you. Do any of you use > ammonia water to soften the blocks before cutting? If so, does this have any > effect on IHC staining? Any help will be greatly appreciated. Thanks. > > Sharon Davis-Devine, CT (ASCP) > Cytology-Histology Supervisor > Carle Foundation Hospital > Laboratory and Pathology Services > 611 West Park Street > Urbana, Illinois 61801 > 217-383-3572 > sharon.davis-devine@carle.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mmaglion <@t> unch.unc.edu Tue Dec 28 12:52:48 2010 From: mmaglion <@t> unch.unc.edu (Maglione, Marilou (Muscato)) Date: Tue Dec 28 12:52:48 2010 Subject: [Histonet] Topic 5: Tissue Processor Selection In-Reply-To: References: Message-ID: Hi Histonetters, I thought I'd weigh in on the question about tissue processors...I've been a 'lurker' and have enjoyed everyone's helpful posts. Thanks! We have had VIP's for many years, with dependable service. But through our efforts to make our lab Lean and more efficient, we've added 3 Peloris processors over the past 2 years. The tissue quality is excellent and the processing times are less than half that of our VIP's. We process fatty tissues in 5.5 hours now and most rushes in 45 min. Each Peloris can function as two separate processors, so for a small lab one retort could be used for rushes while a full-length program is running in the other. We also use less reagent because the Peloris tells us when to change the solutions, helping us avoid extreme variations in reagent quality and wasting reagents that are still good. Our pathologists have been very happy with the quality and the TAT and everyone finds it really easy to use. Marilou Maglione CT (ASCP), HTL UNC Healthcare Chapel Hill, NC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, December 28, 2010 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 85, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject (Kim.Donadio@bhcpns.org) 2. Chuck Churukian's Address (Akemi Allison) 3. Use of ammonia water (Sharon.Davis-Devine) 4. What New Tissue Processor Should we get ? (Parker, Helayne) 5. RE: What New Tissue Processor Should we get ? (Nails, Felton) 6. Listing (M.N.) 7. RE: Use of ammonia water (Susan.Walzer@HCAHealthcare.com) 8. RE: Use of ammonia water (Bartlett, Jeanine (CDC/OID/NCEZID)) 9. RE: MPB antibody (Settembre, Dana) 10. bouin solution (wassan alkadhumi) 11. AW: [Histonet] bouin solution (Gudrun Lang) 12. Re: Use of ammonia water (Victoria Baker) ---------------------------------------------------------------------- Message: 1 Date: Mon, 27 Dec 2010 14:28:24 -0600 From: Kim.Donadio@bhcpns.org Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject To: WILLIAM DESALVO Cc: histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Do others document separately the original creation, training, competency, implementation of procedures/processes and then continual yearly training, competency and review of those processes? Are you using a Quality Management System? How about Electronic system to manage? Yes, to all of those. We document original training and then all competencies are part of their yearly eval. We also have the QMS electronic policy/procedure Data base that sends each individual reminders when they need to read a update or sign off on the yearly requirement that they have read and understand the material. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 WILLIAM DESALVO Sent by: histonet-bounces@lists.utsouthwestern.edu 12/23/2010 10:49 AM To , histonet cc Subject RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject Thanks, I am aware of Peggy's form. We have similar hard copy forms now, but we are moving to an electronic system. It will be much more manageable for 125 employees. When you state in your form the employee is competent, do you have documentation to support that there was a competency evaluation? We only state the employee has read and understands. We have separate documentation for training and competency. I love this type of discussion string. There are so many different processes out in Histoland. Do others document separately the original creation, training, competency, implementation of procedures/processes and then continual yearly training, competency and review of those processes? Are you using a Quality Management System? How about Electronic system to manage? William DeSalvo, B.S., HTL(ASCP) > From: RCazares@schosp.org > To: wdesalvo.cac@hotmail.com > Date: Thu, 23 Dec 2010 10:17:48 -0600 > Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > Hello histonetters, > > When I implement a new procedure in my lab, besides the routine yearly sign-off sheet that accompanies the procedure, there an additional sheet that states > > " The following employees have reviewed this procedure and by signing below are acknowledging that they understand and are competent in performing the procedure:" > > Below that statement is a box with signature and date lines for all the employees using this procedure to sign. This makes each employee responsible for reading and understanding the procedure. This I got from one of Peggy Wenk's workshops (Thanks Peggy!!). I can send a copy to anyone who'd like one, just contact me directly. > > > Ruth Cazares, HT (ASCP) > Histology Supervisor > Department of Pathology > Swedish Covenant Hospital > Chicago, IL > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Thursday, December 23, 2010 7:27 AM > To: bsullivan@shorememorial.org; Victor Tobias > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > > Beatrice, > How do you show proof to CAP that your employees that use the procedures and perform the tasks described in the procedures have reviewed and understand the procedure when there is a new one during your cycle year and before it is implemented? > > CAP has changed the way they inspect and they are now looking for how whether the employees performing a task described in the procedure understands the procedure and is performing exactly as the procedure describes. They are not as concerned that the Supervisor and management knows about the procedures, they want to see how informed, trained and competent the bench employee is. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > To: victor@pathology.washington.edu > > From: BSullivan@shorememorial.org > > Date: Thu, 23 Dec 2010 07:53:22 -0500 > > Subject: Re: [Histonet] CAP Question regarding procedure manual > > CC: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > > > Victor, > > To my knowledge all you need is proof that the staff reviewed the manuals. > > We accomplish this by a sign off sheet in the front of each manual we use. > > The Supervisor, or designee, needs to review and sign off on each procedure > > in each manual. > > > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > > AP Supervisor > > Shore Memorial Hospital > > 609-653-3590 > > > > > > Speak only well of people and you need never whisper > > > > > > > > Victor Tobias > > > .washington.edu> To > > Sent by: Histonet > > histonet-bounces@ > > lists.utsouthwest cc > > ern.edu > > Subject > > [Histonet] CAP Question regarding > > 12/22/2010 06:16 procedure manual > > PM > > > > > > > > > > > > > > > > > > > > Is there a requirement to have a signature of every staff member on a > > procedure if they perform that procedure in a manual? Wouldn't one > > signature on a cover page suffice that you have read and understand what > > is in the manual? > > > > Victor > > > > -- > > Victor Tobias > > Clinical Applications Analyst > > University of Washington Medical Center > > Dept of Pathology Room BB220 > > 1959 NE Pacific > > Seattle, WA 98195 > > victor@pathology.washington.edu > > 206-598-2792 > > 206-598-7659 Fax > > ================================================= > > Privileged, confidential or patient identifiable information may be > > contained in this message. This information is meant only for the use > > of the intended recipients. If you are not the intended recipient, or > > if the message has been addressed to you in error, do not read, > > disclose, reproduce, distribute, disseminate or otherwise use this > > transmission. Instead, please notify the sender by reply e-mail, and > > then destroy all copies of the message and any attachments. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > > Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 2 Date: Mon, 27 Dec 2010 12:57:31 -0800 (PST) From: Akemi Allison Subject: [Histonet] Chuck Churukian's Address To: Histonet Message-ID: <563441.88990.qm@web113805.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All, I just spoke with Chuck a few minutes ago and his spirits are up.? He?is working with a physical therapist this afternoon.? He gave me permission to post his address, and would love to hear from those of you who would like to send him cards.? I think it would really cheer him up.? His address is: 34 Gailhaven Court Rochester, NY 14618 ?Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ------------------------------ Message: 3 Date: Mon, 27 Dec 2010 14:58:20 -0600 From: Sharon.Davis-Devine Subject: [Histonet] Use of ammonia water To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com ------------------------------ Message: 4 Date: Mon, 27 Dec 2010 15:15:44 -0600 From: "Parker, Helayne" Subject: [Histonet] What New Tissue Processor Should we get ? To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <930EB2E8DF68C544873EDD2A3D5F506004700E6898@email1.skaggs.net> Content-Type: text/plain; charset="us-ascii" Hi All, If given the choice- what would be the best Tissue Processor to get for a small Path Lab. We have always had VIPs. Ours is about 16 y/o and we need to replace it and use it as a back up or for small biopsies. I was looking at the new VIPs and our medical repair people are very happy w/ the VIPs in the past. I am looking at VIP 5 and 6. Do not need anything too fancy. Just want a workhorse that has little or no issues and is user friendly. Any input is much appreciate on these or other products - private emails great ! Thanks, Helayne Parker hparker@skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ------------------------------ Message: 5 Date: Mon, 27 Dec 2010 15:19:37 -0600 From: "Nails, Felton" Subject: [Histonet] RE: What New Tissue Processor Should we get ? To: "'Parker, Helayne'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset=us-ascii As have you, I have also had great service from my VIP's and plan on purchasing the VIP 6. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Monday, December 27, 2010 3:16 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] What New Tissue Processor Should we get ? Hi All, If given the choice- what would be the best Tissue Processor to get for a small Path Lab. We have always had VIPs. Ours is about 16 y/o and we need to replace it and use it as a back up or for small biopsies. I was looking at the new VIPs and our medical repair people are very happy w/ the VIPs in the past. I am looking at VIP 5 and 6. Do not need anything too fancy. Just want a workhorse that has little or no issues and is user friendly. Any input is much appreciate on these or other products - private emails great ! Thanks, Helayne Parker hparker@skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== ------------------------------ Message: 6 Date: Mon, 27 Dec 2010 22:04:46 -0800 From: "M.N." Subject: [Histonet] Listing To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi - I am looking for someone in the LA area (ideally someone who works in a lab or hospital setting) to teach me how to process biopsies from start to finish, and to cut paraffin-embedded sections. I would compensate you for your time, of course. Please contact me with your availability - I'd like to get started soon. Thanks! ------------------------------ Message: 7 Date: Tue, 28 Dec 2010 01:57:56 -0600 From: Subject: [Histonet] RE: Use of ammonia water To: , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2B3D09D7CA@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" We have always used water with liquid soap and some ammonia to soften blocks. It has had no effect on IHC. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Monday, December 27, 2010 3:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Use of ammonia water Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 28 Dec 2010 05:51:55 -0500 From: "Bartlett, Jeanine (CDC/OID/NCEZID)" Subject: RE: [Histonet] Use of ammonia water To: "Sharon.Davis-Devine" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii I use it on particularly difficult blocks and have seen no ill effects. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Monday, December 27, 2010 3:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Use of ammonia water Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 28 Dec 2010 09:40:19 -0500 From: "Settembre, Dana" Subject: [Histonet] RE: MPB antibody To: "'Taylor, Jean'" , "'ihcrg@googlegroups.com'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Jean, I use Leica's MBP which the get from NovoCastra cat. NCL-MBP I use it on formalin fixed paraffin embedded human tissue on my Dako. I use Dako's Target Ret. Soln in a steamer for 40 minutes Incubate 15 min with it at the dilution of 1:25. Then I use Dako LSAB2 kit. It works fine. Good luck, Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Thursday, December 23, 2010 2:14 PM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] MPB antibody Hi Everyone, Has anyone worked with the MBP (human eosinophilic major basic protein) antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 28 Dec 2010 06:49:54 -0800 (PST) From: wassan alkadhumi Subject: [Histonet] bouin solution To: histonet@lists.utsouthwestern.edu Message-ID: <604286.3701.qm@web45206.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Histonet I have question concerning?bouin solution, we use it in masson trichrome before starting the stain.?we incubate the slides trimmed at 3 micron in bouin solution for 18 hr in room temp which is not controlled.?Then?we wash the slides?using running water first and?then distilled water, but?according to what i?red,?when?using bouin solution,the tissue?should?be washed with (50-70)% alcohol?to prevent deterioration of staining in time. now i should mention?that in the?our ?masson trichrome?procedure?there is no washing with alcohol?but only with water. what should i do? In the book its mentioned that we have to wash with alcohol if the tissue is not processed yet, is that why the alcohol step is?missing in masson tri procedure?and why will the stain deteriorate if some of the bouin solution stayed in the tissue? The?main fixative that is used in our lab for all grooses and biopsy is 10% formalin (except bone marrow biopsy). bouin solution is used after processing of the tissue and deparaffinization of the sections is finished. Thanks Wassan Histotechnician Shorh hospital North of Iraq ------------------------------ Message: 11 Date: Tue, 28 Dec 2010 17:42:52 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] bouin solution To: "'wassan alkadhumi'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <2CFC6E180EFB469AA061237DC32D690B@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" We incubate the trichrome-slides after deparaffination for 2 hours in 56?C, then rinse in warm tapwater until the slides are colourless. Then another rinse in distilled water, because I don't want carry over from tapwater into the weigert's iron hematoxylin. ... If you let bouins in the tissue, it acts like a stain. Think of vanGieson staining solution (also called picrofuchsin). It sounds like the processing after bouin-fixation and the trichrom staining are mixed up. Or the protocols of trichrom-staining of NBF-fixed and bouin-fixed tissue are mixed up. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von wassan alkadhumi Gesendet: Dienstag, 28. Dezember 2010 15:50 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] bouin solution Dear Histonet I have question concerning?bouin solution, we use it in masson trichrome before starting the stain.?we incubate the slides trimmed at 3 micron in bouin solution for 18 hr in room temp which is not controlled.?Then?we wash the slides?using running water first and?then distilled water, but?according to what i?red,?when?using bouin solution,the tissue?should?be washed with (50-70)% alcohol?to prevent deterioration of staining in time. now i should mention?that in the?our ?masson trichrome?procedure?there is no washing with alcohol?but only with water. what should i do? In the book its mentioned that we have to wash with alcohol if the tissue is not processed yet, is that why the alcohol step is?missing in masson tri procedure?and why will the stain deteriorate if some of the bouin solution stayed in the tissue? The?main fixative that is used in our lab for all grooses and biopsy is 10% formalin (except bone marrow biopsy). bouin solution is used after processing of the tissue and deparaffinization of the sections is finished. Thanks Wassan Histotechnician Shorh hospital North of Iraq _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 28 Dec 2010 12:47:13 -0500 From: Victoria Baker Subject: Re: [Histonet] Use of ammonia water To: "Sharon.Davis-Devine" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Sharon, I use a 1.5% solution for bone marrow cases and bloody tissue. It does also work well for GI. I've never had an issue with it affecting any of my IHC. An alternative though (when I can't get my hands on ammonium hydroxide) is to put these blocks in warm water (I'm careful about the waterbath because of X-contamination issues) for about 30-45 seconds and this works just as well. I stopped using any surface decal solution or Nair as I did see some differences with the expression of certain markers - I did not see any false negs, but the staining itself was not as intense it looked like. Vikki On Mon, Dec 27, 2010 at 3:58 PM, Sharon.Davis-Devine < Sharon.Davis-Devine@carle.com> wrote: > Happy Holidays Histonetters, I have a question for you. Do any of you use > ammonia water to soften the blocks before cutting? If so, does this have any > effect on IHC staining? Any help will be greatly appreciated. Thanks. > > Sharon Davis-Devine, CT (ASCP) > Cytology-Histology Supervisor > Carle Foundation Hospital > Laboratory and Pathology Services > 611 West Park Street > Urbana, Illinois 61801 > 217-383-3572 > sharon.davis-devine@carle.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 85, Issue 26 **************************************** From alisha <@t> ka-recruiting.com Tue Dec 28 15:10:21 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Dec 28 15:09:18 2010 Subject: [Histonet] Experienced Histotech and Histology Supervisor Jobs Message-ID: <322850652.1293570621270.JavaMail.cfservice@SL4APP4> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From AnthonyH <@t> chw.edu.au Tue Dec 28 15:51:53 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Dec 28 15:52:03 2010 Subject: [Histonet] Chuck Churukian In-Reply-To: <034BC67A-78AE-4299-9BAC-D4922BF1A891@yahoo.com> References: <034BC67A-78AE-4299-9BAC-D4922BF1A891@yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71570358AB@xmdb02.nch.kids> What disturbing news. Hopefully Chuck can fight on and be back to full strength by April. All our prayers and support are for Chuck and his family. A father figure in Histochemistry. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Monday, 27 December 2010 12:12 PM To: Histonet Subject: [Histonet] Chuck Churukian I just heard from Irene Churukian today. Please put Chuck in your thoughts and prayers. Irene said this: CLL cells changed in character so Chuck began chemo treatments on Dec. 1. His second one was Dec. 21... He has six altogether - three weeks apart. The first cycle was difficult... he was very weak.... in bed most of the time. He is now improving ... thankfully. He has never been sick and confined to bed/home so he gets cabin fever easily. People have been so kind to visit him. So our Christmas this year was very different from years past. We are hoping and praying that by April, he will be in remission after all the treatments are completed. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From jkrupp <@t> deltacollege.edu Tue Dec 28 17:22:54 2010 From: jkrupp <@t> deltacollege.edu (Jonathan Krupp) Date: Tue Dec 28 17:23:06 2010 Subject: [Histonet] Fwd: Nor Cal Histotech program In-Reply-To: Message-ID: <531815146.272564.1293578574576.JavaMail.root@mercury.deltacollege.edu> Hi I am new here. I am part of an EM tech training program at Delta College in Stockton, CA. We train students to do EM, fixing, sectioning, staining, and instrument operation. Some of my students have been asking about LM histotech sort of things. They want to know more about histo techniques in general and maybe broadening their job prospects beyond EM. I am exploring the possibilities of adding more LM histotech things to the program and seek advice. Is there room for another program? Is seeking accreditation worth while? What would I need to get set up? Are there jobs available for graduates? Just getting started, your advice will be invaluable. Jon Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu From lpwenk <@t> sbcglobal.net Tue Dec 28 18:48:02 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Dec 28 18:48:07 2010 Subject: [Histonet] Fwd: Nor Cal Histotech program In-Reply-To: <531815146.272564.1293578574576.JavaMail.root@mercury.deltacollege.edu> References: <531815146.272564.1293578574576.JavaMail.root@mercury.deltacollege.edu> Message-ID: <587BDAFD5B6A4379A22840FB4B8A03A0@HP2010> There is SUCH a need for histotech programs. Currently, there are only 39 histologic technician (HT) (most community college based) and 4 histotechnologist (HTL) (need 4 year degree). And there are shortages everywhere. There are 2 HT programs in California. One in Berkley, one in Walnut. The accrediting agency is National Accrediting Agency for Clinical Laboratory Sciences (NAACLS). www.naacls.org For information on how to contact the two California programs, once on the NAACLS website, - on the left, click on Find a Program - Under Program Type, click on HT & hit Search. Programs are listed alphabetically by state. - If you want to talk with HTL program directors, follow above directions, except click on HTL. Any program director would be willing to give you more information. If you look under "accreditation" on the left, there is HT and HTL. Click on one, and you can find the accreditation standards you would have to follow. There are a few standards that are different between HT and HTL, so you need to look at both. The advantage of being an accredited HT or HTL program is that the students are eligible to take the national certification exam through American Society for Clinical Pathology (ASCP), immediately upon successful completion of the NAACLS accredited HT or HTL program. However, since you are just starting out, and look like you need lots of information, I'm going to suggest going to the National Society for Histotechnology (NSH) website. More information about what histotechs are, about meetings, state society presidents, jobs board, membership with journal specific for histology/tissue, etc. There is also a 3 hour continuing education you can purchase, titled "How to Start an Accredited HT or HTL Program in either a College or a Hospital". I presented it at the NSH Symposium in 2008, and it was recorded with the PowerPoint, so you can listen to me and look at the PowerPoint. It has lots of advantages and disadvantages of starting different types of programs, contacts, proof that we need more programs, etc. Granted, the information is 3 years old, and there are a lot more HT and HTL program than there were 3 years ago, but most of the other information is the same. Just a disclaimer, I don't make any money from this. I had presented it every year from 1999-2008, and NSH thought it might work as an on-line module, for people like yourself who need the information at times other than in the fall when the NSH symposium is. Go to www.nsh.org - Across the top, click on Professional Development - About the 5th one down, click on On-Line Learning Center - Click on Meeting Content - Scroll down and click on NSH 2008 Annual Symposium/Convention in Pittsburgh, PA - Click on #27 = How to Start an Accredited HT or HTL program Once you get some information under your belt, I'd be more than happy to answer questions specific for your situation. Just look on the NAACLS web page, under HT or HTL (we have both programs), and you'll find me in Michigan. :-) Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Jonathan Krupp" Sent: Tuesday, December 28, 2010 6:22 PM To: Subject: [Histonet] Fwd: Nor Cal Histotech program > > > > Hi > > I am new here. I am part of an EM tech training program at Delta College > in Stockton, CA. > > We train students to do EM, fixing, sectioning, staining, and instrument > operation. > > Some of my students have been asking about LM histotech sort of things. > They want to know more about histo techniques in general and maybe > broadening their job prospects beyond EM. > > I am exploring the possibilities of adding more LM histotech things to the > program and seek advice. Is there room for another program? Is seeking > accreditation worth while? What would I need to get set up? Are there jobs > available for graduates? > > Just getting started, your advice will be invaluable. > > Jon > > Jonathan Krupp > Delta College > 5151Pacific Ave. > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Tue Dec 28 19:18:03 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Dec 28 19:18:07 2010 Subject: [Histonet] bouin solution In-Reply-To: <604286.3701.qm@web45206.mail.sp1.yahoo.com> References: <604286.3701.qm@web45206.mail.sp1.yahoo.com> Message-ID: If the tissue is in Bouins, the excess Bouins needs to be washed out of the tissue immediately after fixation. Water could be used to rinse out the excess picric acid, but I think some people are worried that there could be some cell swelling if the tissue is left in the water too long (particularly if the tissue is underfixed/not stablized enough). If I remember correctly, too high of a percent of alcohol (95%, for example), will cause the picric acid salts to precipitate in the tissue, and it would be like trying to microtome tissue with sand in it. So 50-70% alcohol is a good compromise. Have enough water to remove the excess picric acid salts, but not too much water to cause tissue swelling, and not too much percent of alcohol to cause picric acid salts to precipitate in the tissue. For your slides already microtomed and on slides - these have already been fixed and processed. If the tissue was fixed in a picric acid fixative like Bouins, there is no need for the step of putting the slide in Bouins. If the tissue was fixed in something else (10% neutral buffered formalin, for example), then the slide/tissue needs to be placed into Bouins in order for the trichrome stains to work correctly. This is called either post-fixing or post-mordanting. There are several theories as to why the Bouins is needed for the trichromes (change tissue charges, change tissue density), but the end result is that Bouins fixed or Bouins post-mordanted tissue have muscles that are red and collagen that are blue or green (depending upon which dye you use). If the NBF fixed tissue is not post-mordanted, the red is a red/blue or red/green, and the collagen has strands of red in it. In other words, the colors are not as true or as crisp. Now, the slides that were post-mordanted in Bouins have tissue that is yellow. We need to get the yellow out. Not so much because of the picrate salts that precipitate, but more because the yellow color would interfere with the reds and the blues or greens colors that you are trying to make. The tissue is only 3-5 um thick, so it won't take as long to remove the color. And the tissue has been well fixed, dehydrated and cleared during tissue processing. So there are lots of cross-links to stabilize the tissue. So rinsing in water is only going to take a few minutes, and the tissue is stabilized, so there shouldn't be any cell size changes. You could rinse in 50-70% alcohol, but that's only 50-30% water, so it is going to take a lot longer to get rid of the yellow color than rinsing in water. Rinsing in tap water is fine for most labs, as long as the quality of tap water is good (not too acidic or basic, not too much iron or sulfur, no microorganisms). I like to rinse with d. water before any stain, just in case there is any contaminating metal that might interfere with the stains (we have a lot of iron in our part of the state). By the way, putting the slides in Bouins in a coplin jar with a lid screwed on, then putting in a 60 degree C. oven or water bath for 45-60 minutes works great. This would reduce your turn around time by 17 hours! Two words of caution. 1) Don't breath in the hot Bouins fumes (picric acid, acetic acid, formaldehyde). Open up the coplin jar in a hood. 2) Don't stick the hot coplin jar into cold running water. Coplin jar will break/crack. We usually take the slides out of the hot Bouins, and put the slides into a new coplin jar with water, then put this under the running water. The lid is put back on the Bouins coplin jar, and allowed to cool. Some places reuse the Bouins for a number of times. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "wassan alkadhumi" Sent: Tuesday, December 28, 2010 9:49 AM To: Subject: [Histonet] bouin solution > Dear Histonet > I have question concerning bouin solution, we use it in masson trichrome > before > starting the stain. we incubate the slides trimmed at 3 micron in bouin > solution > for 18 hr in room temp which is not controlled. Then we wash the slides > using > running water first and then distilled water, but according to what > i red, when using bouin solution,the tissue should be washed with (50-70)% > alcohol to prevent deterioration of staining in time. now i should mention > that > in the our masson trichrome procedure there is no washing with alcohol > but only > with water. > > what should i do? In the book its mentioned that we have to wash with > alcohol if > the tissue is not processed yet, is that why the alcohol step is missing > in > masson tri procedure?and why will the stain deteriorate if some of the > bouin > solution stayed in the tissue? > The main fixative that is used in our lab for all grooses and biopsy is > 10% > formalin (except bone marrow biopsy). bouin solution is used after > processing of > the tissue and deparaffinization of the sections is finished. > Thanks > > Wassan > Histotechnician > Shorh hospital > North of Iraq > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim.tournear <@t> yahoo.com Wed Dec 29 07:10:01 2010 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Wed Dec 29 07:10:05 2010 Subject: [Histonet] Florida State Histology License Message-ID: <313964.32140.qm@web120203.mail.ne1.yahoo.com> Can any one tell me how to go about getting a Florida State Histology License? And I understand that there is a test to take for the Supervisor's license as well....I'll take info for that too,,,,thanks in advance!! ~Kim~? ? OU ROCKS!!!! ~Don't be afraid your life will end, be afraid it will never begin~ From Ronald.Houston <@t> nationwidechildrens.org Wed Dec 29 08:12:50 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Dec 29 08:13:00 2010 Subject: [Histonet] CAP question for pediatric pathology labs Message-ID: CYP.00190 Phase I N/A YES NO For laboratories that perform non-gynecologic cytopathology, does the laboratory participate in a peer educational program in NON-GYNECOLOGIC cytopathology (e.g., CAP Interlaboratory Comparison Program in Non-Gynecologic Cytopathology NGC)? How are labs responding to this request for a peer-educational program in non-gyn cytopath, as there is no such program for pediatric cytopathology, and, in the opinion of our pathologists, the CAP program is useless as far as it pertains to pediatrics? We have had varying responses from inspectors which we have adopted and then the next inspector says this isn't good enough and you should be doing something else. There has been little to no direction from CAP. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From tahseen <@t> brain.net.pk Wed Dec 29 08:23:28 2010 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Wed Dec 29 08:23:37 2010 Subject: [Histonet] AgNORs staining prtocol & UVC lamp safeguard Message-ID: <25574.203.135.35.66.1293632608.squirrel@brain.net.pk> Dear All, 1 Would you like to share your AgNORs staining prtocol with me? 2 We are using Leica CM1850 US Cryostat, we are going to use UVC lamp for disinfection, so what kind of safeguard are recommended other then sliding window has been properly closed. Thanks advance Muhammad Tahseen Senior Supervisore Histopathology SKMCH & RC Lahore Pakistan. From histotech <@t> imagesbyhopper.com Wed Dec 29 09:42:56 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Dec 29 09:43:13 2010 Subject: [Histonet] Florida State Histology License In-Reply-To: <313964.32140.qm@web120203.mail.ne1.yahoo.com> References: <313964.32140.qm@web120203.mail.ne1.yahoo.com> Message-ID: <002a01cba76f$17e1bef0$47a53cd0$@imagesbyhopper.com> Kim, You will have to go to the Florida Department of Health website to get the application. For a supervisor license, you will need to meet specific requirements and then take an additional 24 hours of CEUs and pay the license fee. Please click on the following link for more information. http://www.doh.state.fl.us/mqa/ClinLab/index.html There are a number of links on that page, you might wish to begin with the Applicant Information link. Good Luck! Michelle From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Wednesday, December 29, 2010 8:10 AM To: Histonet Subject: [Histonet] Florida State Histology License Can any one tell me how to go about getting a Florida State Histology License? And I understand that there is a test to take for the Supervisor's license as well....I'll take info for that too,,,,thanks in advance!! ~Kim~ OU ROCKS!!!! ~Don't be afraid your life will end, be afraid it will never begin~ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1191 / Virus Database: 1435/3346 - Release Date: 12/29/10 From AHutton <@t> dh.org Wed Dec 29 11:29:23 2010 From: AHutton <@t> dh.org (Hutton, Allison) Date: Wed Dec 29 11:31:26 2010 Subject: [Histonet] billing for breast cases Message-ID: <38A56C4F4630D348A50B3720409270870E0FE294@dhmail.dhorg.org> A debate has arisen and I am looking to histonet for a more definitive answer. We have a breast surgeon who, after he removes the lumpectomy specimen, always goes back and removes more tissue around the margins of the lumpectomy. Our question is how should these additional margins be charged. Currently we charge an 88305 for each of the additional margins (there are no sutures indicating any orientation, however, one can determine the old and new margins). Is 88305 the correct charge in this situation or should they be higher at an 88307? Thank you in advance, Allison From DKBoyd <@t> chs.net Wed Dec 29 11:54:16 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Dec 29 11:54:26 2010 Subject: [Histonet] billing for breast cases In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE294@dhmail.dhorg.org> Message-ID: My understanding is: Inked margins (on any specimen type) are 88307. Doesn't specify who inks the margin. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Hutton, Allison" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/29/2010 12:35 PM To cc Subject [Histonet] billing for breast cases A debate has arisen and I am looking to histonet for a more definitive answer. We have a breast surgeon who, after he removes the lumpectomy specimen, always goes back and removes more tissue around the margins of the lumpectomy. Our question is how should these additional margins be charged. Currently we charge an 88305 for each of the additional margins (there are no sutures indicating any orientation, however, one can determine the old and new margins). Is 88305 the correct charge in this situation or should they be higher at an 88307? Thank you in advance, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From LINDA.MARGRAF <@t> childrens.com Wed Dec 29 12:31:57 2010 From: LINDA.MARGRAF <@t> childrens.com (Linda Margraf) Date: Wed Dec 29 12:32:01 2010 Subject: [Histonet] RE: CAP question for pediatric pathology labs In-Reply-To: References: Message-ID: <683621D7852C2F488898D0AC7F164A98063AD9AA@CMCPBEXMAIL02.Childrens.med> Dear Ronnie We at the Children's Medical Center, Dallas do a twice a year slide exchange of ten cytopathology cases with another Children's Hospital. We actually learn a lot from this exercise as they critique slide quality as well as diagnoses. All the Anatomic pathology faculty take part in case reviews and we meet as a group to hear about the results of the other program's review of our cases. It is also much more pertinent to the nature of our pediatric pathology material than the CAP program would be (and the price is negligible). We have never had a CAP inspector have a problem with this type of program. We are careful to keep it all well documented in our quality records. Linda M Histonet administrator -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, December 29, 2010 8:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question for pediatric pathology labs Importance: High CYP.00190 Phase I N/A YES NO For laboratories that perform non-gynecologic cytopathology, does the laboratory participate in a peer educational program in NON-GYNECOLOGIC cytopathology (e.g., CAP Interlaboratory Comparison Program in Non-Gynecologic Cytopathology NGC)? How are labs responding to this request for a peer-educational program in non-gyn cytopath, as there is no such program for pediatric cytopathology, and, in the opinion of our pathologists, the CAP program is useless as far as it pertains to pediatrics? We have had varying responses from inspectors which we have adopted and then the next inspector says this isn't good enough and you should be doing something else. There has been little to no direction from CAP. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From AHutton <@t> dh.org Wed Dec 29 12:46:18 2010 From: AHutton <@t> dh.org (Hutton, Allison) Date: Wed Dec 29 12:48:22 2010 Subject: [Histonet] RE: billing for breast cases In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE294@dhmail.dhorg.org> Message-ID: <38A56C4F4630D348A50B3720409270870E0FE295@dhmail.dhorg.org> I have gotten many great responses. However, I just want to make a few clarifications based on those responses. All of these specimens are taken at one time. This surgeon is not going back at a later date and taking these margins. Generally the cases are recieved as follows: sentinel nodes, lumpectomy, additional anterior, superior, medial, lateral, deep margins. 90% of the time we do an gross assessment of the margins for the lumpectomy then the additional margins follow. Thanks Allison > -----Original Message----- > From: Hutton, Allison > Sent: Wednesday, December 29, 2010 12:29 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: billing for breast cases > > A debate has arisen and I am looking to histonet for a more definitive answer. We have a breast surgeon who, after he removes the lumpectomy specimen, always goes back and removes more tissue around the margins of the lumpectomy. Our question is how should these additional margins be charged. Currently we charge an 88305 for each of the additional margins (there are no sutures indicating any orientation, however, one can determine the old and new margins). Is 88305 the correct charge in this situation or should they be higher at an 88307? > Thank you in advance, > Allison From JWeems <@t> sjha.org Wed Dec 29 13:51:40 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Dec 29 13:51:45 2010 Subject: [Histonet] RE: billing for breast cases In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE295@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870E0FE294@dhmail.dhorg.org> <38A56C4F4630D348A50B3720409270870E0FE295@dhmail.dhorg.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B333131@CHEXCMS10.one.ads.che.org> Are you doing frozens? I don't understand why he would go back without microscopic confirmation of need. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Wednesday, December 29, 2010 13:46 To: Hutton, Allison; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: billing for breast cases I have gotten many great responses. However, I just want to make a few clarifications based on those responses. All of these specimens are taken at one time. This surgeon is not going back at a later date and taking these margins. Generally the cases are recieved as follows: sentinel nodes, lumpectomy, additional anterior, superior, medial, lateral, deep margins. 90% of the time we do an gross assessment of the margins for the lumpectomy then the additional margins follow. Thanks Allison > -----Original Message----- > From: Hutton, Allison > Sent: Wednesday, December 29, 2010 12:29 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: billing for breast cases > > A debate has arisen and I am looking to histonet for a more definitive answer. We have a breast surgeon who, after he removes the lumpectomy specimen, always goes back and removes more tissue around the margins of the lumpectomy. Our question is how should these additional margins be charged. Currently we charge an 88305 for each of the additional margins (there are no sutures indicating any orientation, however, one can determine the old and new margins). Is 88305 the correct charge in this situation or should they be higher at an 88307? > Thank you in advance, > Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Bill.Tench <@t> pph.org Wed Dec 29 14:10:50 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Dec 29 14:10:56 2010 Subject: [Histonet] margins without frozen Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A596F@MAIL1.pph.local> If the margin is grossly close, then re-excision without a frozen is entirely appropriate. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From HParker <@t> Skaggs.Net Wed Dec 29 14:26:22 2010 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Wed Dec 29 14:25:54 2010 Subject: [Histonet] Bouins and the Trichome In-Reply-To: <388fca0f-5bb3-4956-823c-f09ae42ef3dc@email1.skaggs.net> References: <388fca0f-5bb3-4956-823c-f09ae42ef3dc@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F506004700E68AE@email1.skaggs.net> We have a stain through Sigma (and we change out solutions from Poly Sci too) that we place the slides in a plastic coplin jar w/ Bouins (w/a hole drilled in the top) and microwave for 25 seconds and let set for 5 mins. Then run slides in running water until clear. Tap water is fine around here. Then at that point we continue the stain at room temperature w/ regular times. It is a Masson method and is turns out very nicely- very deep rich reds and blues. Helayne Parker, HT, (A.S.C.P.) Skaggs Regional Medical Center Branson, Missouri -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, December 29, 2010 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 85, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Topic 5: Tissue Processor Selection (Maglione, Marilou (Muscato)) 2. Experienced Histotech and Histology Supervisor Jobs (Alisha Dynan) 3. RE: Chuck Churukian (Tony Henwood) 4. Fwd: Nor Cal Histotech program (Jonathan Krupp) 5. Re: Fwd: Nor Cal Histotech program (Lee & Peggy Wenk) 6. Re: bouin solution (Lee & Peggy Wenk) 7. Florida State Histology License (Kim Tournear) 8. CAP question for pediatric pathology labs (Houston, Ronald) 9. AgNORs staining prtocol & UVC lamp safeguard (tahseen@brain.net.pk) 10. RE: Florida State Histology License (histotech@imagesbyhopper.com) 11. billing for breast cases (Hutton, Allison) 12. Re: billing for breast cases (DKBoyd@chs.net) ---------------------------------------------------------------------- Message: 1 Date: Tue, 28 Dec 2010 13:52:48 -0500 From: "Maglione, Marilou (Muscato)" Subject: [Histonet] Topic 5: Tissue Processor Selection To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 Hi Histonetters, I thought I'd weigh in on the question about tissue processors...I've been a 'lurker' and have enjoyed everyone's helpful posts. Thanks! We have had VIP's for many years, with dependable service. But through our efforts to make our lab Lean and more efficient, we've added 3 Peloris processors over the past 2 years. The tissue quality is excellent and the processing times are less than half that of our VIP's. We process fatty tissues in 5.5 hours now and most rushes in 45 min. Each Peloris can function as two separate processors, so for a small lab one retort could be used for rushes while a full-length program is running in the other. We also use less reagent because the Peloris tells us when to change the solutions, helping us avoid extreme variations in reagent quality and wasting reagents that are still good. Our pathologists have been very happy with the quality and the TAT and everyone finds it really easy to use. Marilou Maglione CT (ASCP), HTL UNC Healthcare Chapel Hill, NC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, December 28, 2010 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 85, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject (Kim.Donadio@bhcpns.org) 2. Chuck Churukian's Address (Akemi Allison) 3. Use of ammonia water (Sharon.Davis-Devine) 4. What New Tissue Processor Should we get ? (Parker, Helayne) 5. RE: What New Tissue Processor Should we get ? (Nails, Felton) 6. Listing (M.N.) 7. RE: Use of ammonia water (Susan.Walzer@HCAHealthcare.com) 8. RE: Use of ammonia water (Bartlett, Jeanine (CDC/OID/NCEZID)) 9. RE: MPB antibody (Settembre, Dana) 10. bouin solution (wassan alkadhumi) 11. AW: [Histonet] bouin solution (Gudrun Lang) 12. Re: Use of ammonia water (Victoria Baker) ---------------------------------------------------------------------- Message: 1 Date: Mon, 27 Dec 2010 14:28:24 -0600 From: Kim.Donadio@bhcpns.org Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject To: WILLIAM DESALVO Cc: histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Do others document separately the original creation, training, competency, implementation of procedures/processes and then continual yearly training, competency and review of those processes? Are you using a Quality Management System? How about Electronic system to manage? Yes, to all of those. We document original training and then all competencies are part of their yearly eval. We also have the QMS electronic policy/procedure Data base that sends each individual reminders when they need to read a update or sign off on the yearly requirement that they have read and understand the material. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 WILLIAM DESALVO Sent by: histonet-bounces@lists.utsouthwestern.edu 12/23/2010 10:49 AM To , histonet cc Subject RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject Thanks, I am aware of Peggy's form. We have similar hard copy forms now, but we are moving to an electronic system. It will be much more manageable for 125 employees. When you state in your form the employee is competent, do you have documentation to support that there was a competency evaluation? We only state the employee has read and understands. We have separate documentation for training and competency. I love this type of discussion string. There are so many different processes out in Histoland. Do others document separately the original creation, training, competency, implementation of procedures/processes and then continual yearly training, competency and review of those processes? Are you using a Quality Management System? How about Electronic system to manage? William DeSalvo, B.S., HTL(ASCP) > From: RCazares@schosp.org > To: wdesalvo.cac@hotmail.com > Date: Thu, 23 Dec 2010 10:17:48 -0600 > Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > Hello histonetters, > > When I implement a new procedure in my lab, besides the routine yearly sign-off sheet that accompanies the procedure, there an additional sheet that states > > " The following employees have reviewed this procedure and by signing below are acknowledging that they understand and are competent in performing the procedure:" > > Below that statement is a box with signature and date lines for all the employees using this procedure to sign. This makes each employee responsible for reading and understanding the procedure. This I got from one of Peggy Wenk's workshops (Thanks Peggy!!). I can send a copy to anyone who'd like one, just contact me directly. > > > Ruth Cazares, HT (ASCP) > Histology Supervisor > Department of Pathology > Swedish Covenant Hospital > Chicago, IL > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Thursday, December 23, 2010 7:27 AM > To: bsullivan@shorememorial.org; Victor Tobias > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject > > > Beatrice, > How do you show proof to CAP that your employees that use the procedures and perform the tasks described in the procedures have reviewed and understand the procedure when there is a new one during your cycle year and before it is implemented? > > CAP has changed the way they inspect and they are now looking for how whether the employees performing a task described in the procedure understands the procedure and is performing exactly as the procedure describes. They are not as concerned that the Supervisor and management knows about the procedures, they want to see how informed, trained and competent the bench employee is. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > To: victor@pathology.washington.edu > > From: BSullivan@shorememorial.org > > Date: Thu, 23 Dec 2010 07:53:22 -0500 > > Subject: Re: [Histonet] CAP Question regarding procedure manual > > CC: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > > > Victor, > > To my knowledge all you need is proof that the staff reviewed the manuals. > > We accomplish this by a sign off sheet in the front of each manual we use. > > The Supervisor, or designee, needs to review and sign off on each procedure > > in each manual. > > > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > > AP Supervisor > > Shore Memorial Hospital > > 609-653-3590 > > > > > > Speak only well of people and you need never whisper > > > > > > > > Victor Tobias > > > .washington.edu> To > > Sent by: Histonet > > histonet-bounces@ > > lists.utsouthwest cc > > ern.edu > > Subject > > [Histonet] CAP Question regarding > > 12/22/2010 06:16 procedure manual > > PM > > > > > > > > > > > > > > > > > > > > Is there a requirement to have a signature of every staff member on a > > procedure if they perform that procedure in a manual? Wouldn't one > > signature on a cover page suffice that you have read and understand what > > is in the manual? > > > > Victor > > > > -- > > Victor Tobias > > Clinical Applications Analyst > > University of Washington Medical Center > > Dept of Pathology Room BB220 > > 1959 NE Pacific > > Seattle, WA 98195 > > victor@pathology.washington.edu > > 206-598-2792 > > 206-598-7659 Fax > > ================================================= > > Privileged, confidential or patient identifiable information may be > > contained in this message. This information is meant only for the use > > of the intended recipients. If you are not the intended recipient, or > > if the message has been addressed to you in error, do not read, > > disclose, reproduce, distribute, disseminate or otherwise use this > > transmission. Instead, please notify the sender by reply e-mail, and > > then destroy all copies of the message and any attachments. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > > Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 2 Date: Mon, 27 Dec 2010 12:57:31 -0800 (PST) From: Akemi Allison Subject: [Histonet] Chuck Churukian's Address To: Histonet Message-ID: <563441.88990.qm@web113805.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All, I just spoke with Chuck a few minutes ago and his spirits are up.? He?is working with a physical therapist this afternoon.? He gave me permission to post his address, and would love to hear from those of you who would like to send him cards.? I think it would really cheer him up.? His address is: 34 Gailhaven Court Rochester, NY 14618 ?Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ------------------------------ Message: 3 Date: Mon, 27 Dec 2010 14:58:20 -0600 From: Sharon.Davis-Devine Subject: [Histonet] Use of ammonia water To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com ------------------------------ Message: 4 Date: Mon, 27 Dec 2010 15:15:44 -0600 From: "Parker, Helayne" Subject: [Histonet] What New Tissue Processor Should we get ? To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <930EB2E8DF68C544873EDD2A3D5F506004700E6898@email1.skaggs.net> Content-Type: text/plain; charset="us-ascii" Hi All, If given the choice- what would be the best Tissue Processor to get for a small Path Lab. We have always had VIPs. Ours is about 16 y/o and we need to replace it and use it as a back up or for small biopsies. I was looking at the new VIPs and our medical repair people are very happy w/ the VIPs in the past. I am looking at VIP 5 and 6. Do not need anything too fancy. Just want a workhorse that has little or no issues and is user friendly. Any input is much appreciate on these or other products - private emails great ! Thanks, Helayne Parker hparker@skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ------------------------------ Message: 5 Date: Mon, 27 Dec 2010 15:19:37 -0600 From: "Nails, Felton" Subject: [Histonet] RE: What New Tissue Processor Should we get ? To: "'Parker, Helayne'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset=us-ascii As have you, I have also had great service from my VIP's and plan on purchasing the VIP 6. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Monday, December 27, 2010 3:16 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] What New Tissue Processor Should we get ? Hi All, If given the choice- what would be the best Tissue Processor to get for a small Path Lab. We have always had VIPs. Ours is about 16 y/o and we need to replace it and use it as a back up or for small biopsies. I was looking at the new VIPs and our medical repair people are very happy w/ the VIPs in the past. I am looking at VIP 5 and 6. Do not need anything too fancy. Just want a workhorse that has little or no issues and is user friendly. Any input is much appreciate on these or other products - private emails great ! Thanks, Helayne Parker hparker@skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== ------------------------------ Message: 6 Date: Mon, 27 Dec 2010 22:04:46 -0800 From: "M.N." Subject: [Histonet] Listing To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi - I am looking for someone in the LA area (ideally someone who works in a lab or hospital setting) to teach me how to process biopsies from start to finish, and to cut paraffin-embedded sections. I would compensate you for your time, of course. Please contact me with your availability - I'd like to get started soon. Thanks! ------------------------------ Message: 7 Date: Tue, 28 Dec 2010 01:57:56 -0600 From: Subject: [Histonet] RE: Use of ammonia water To: , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2B3D09D7CA@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" We have always used water with liquid soap and some ammonia to soften blocks. It has had no effect on IHC. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Monday, December 27, 2010 3:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Use of ammonia water Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 28 Dec 2010 05:51:55 -0500 From: "Bartlett, Jeanine (CDC/OID/NCEZID)" Subject: RE: [Histonet] Use of ammonia water To: "Sharon.Davis-Devine" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii I use it on particularly difficult blocks and have seen no ill effects. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Monday, December 27, 2010 3:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Use of ammonia water Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 28 Dec 2010 09:40:19 -0500 From: "Settembre, Dana" Subject: [Histonet] RE: MPB antibody To: "'Taylor, Jean'" , "'ihcrg@googlegroups.com'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Jean, I use Leica's MBP which the get from NovoCastra cat. NCL-MBP I use it on formalin fixed paraffin embedded human tissue on my Dako. I use Dako's Target Ret. Soln in a steamer for 40 minutes Incubate 15 min with it at the dilution of 1:25. Then I use Dako LSAB2 kit. It works fine. Good luck, Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Thursday, December 23, 2010 2:14 PM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] MPB antibody Hi Everyone, Has anyone worked with the MBP (human eosinophilic major basic protein) antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 28 Dec 2010 06:49:54 -0800 (PST) From: wassan alkadhumi Subject: [Histonet] bouin solution To: histonet@lists.utsouthwestern.edu Message-ID: <604286.3701.qm@web45206.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Histonet I have question concerning?bouin solution, we use it in masson trichrome before starting the stain.?we incubate the slides trimmed at 3 micron in bouin solution for 18 hr in room temp which is not controlled.?Then?we wash the slides?using running water first and?then distilled water, but?according to what i?red,?when?using bouin solution,the tissue?should?be washed with (50-70)% alcohol?to prevent deterioration of staining in time. now i should mention?that in the?our ?masson trichrome?procedure?there is no washing with alcohol?but only with water. what should i do? In the book its mentioned that we have to wash with alcohol if the tissue is not processed yet, is that why the alcohol step is?missing in masson tri procedure?and why will the stain deteriorate if some of the bouin solution stayed in the tissue? The?main fixative that is used in our lab for all grooses and biopsy is 10% formalin (except bone marrow biopsy). bouin solution is used after processing of the tissue and deparaffinization of the sections is finished. Thanks Wassan Histotechnician Shorh hospital North of Iraq ------------------------------ Message: 11 Date: Tue, 28 Dec 2010 17:42:52 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] bouin solution To: "'wassan alkadhumi'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <2CFC6E180EFB469AA061237DC32D690B@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" We incubate the trichrome-slides after deparaffination for 2 hours in 56?C, then rinse in warm tapwater until the slides are colourless. Then another rinse in distilled water, because I don't want carry over from tapwater into the weigert's iron hematoxylin. ... If you let bouins in the tissue, it acts like a stain. Think of vanGieson staining solution (also called picrofuchsin). It sounds like the processing after bouin-fixation and the trichrom staining are mixed up. Or the protocols of trichrom-staining of NBF-fixed and bouin-fixed tissue are mixed up. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von wassan alkadhumi Gesendet: Dienstag, 28. Dezember 2010 15:50 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] bouin solution Dear Histonet I have question concerning?bouin solution, we use it in masson trichrome before starting the stain.?we incubate the slides trimmed at 3 micron in bouin solution for 18 hr in room temp which is not controlled.?Then?we wash the slides?using running water first and?then distilled water, but?according to what i?red,?when?using bouin solution,the tissue?should?be washed with (50-70)% alcohol?to prevent deterioration of staining in time. now i should mention?that in the?our ?masson trichrome?procedure?there is no washing with alcohol?but only with water. what should i do? In the book its mentioned that we have to wash with alcohol if the tissue is not processed yet, is that why the alcohol step is?missing in masson tri procedure?and why will the stain deteriorate if some of the bouin solution stayed in the tissue? The?main fixative that is used in our lab for all grooses and biopsy is 10% formalin (except bone marrow biopsy). bouin solution is used after processing of the tissue and deparaffinization of the sections is finished. Thanks Wassan Histotechnician Shorh hospital North of Iraq _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 28 Dec 2010 12:47:13 -0500 From: Victoria Baker Subject: Re: [Histonet] Use of ammonia water To: "Sharon.Davis-Devine" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Sharon, I use a 1.5% solution for bone marrow cases and bloody tissue. It does also work well for GI. I've never had an issue with it affecting any of my IHC. An alternative though (when I can't get my hands on ammonium hydroxide) is to put these blocks in warm water (I'm careful about the waterbath because of X-contamination issues) for about 30-45 seconds and this works just as well. I stopped using any surface decal solution or Nair as I did see some differences with the expression of certain markers - I did not see any false negs, but the staining itself was not as intense it looked like. Vikki On Mon, Dec 27, 2010 at 3:58 PM, Sharon.Davis-Devine < Sharon.Davis-Devine@carle.com> wrote: > Happy Holidays Histonetters, I have a question for you. Do any of you use > ammonia water to soften the blocks before cutting? If so, does this have any > effect on IHC staining? Any help will be greatly appreciated. Thanks. > > Sharon Davis-Devine, CT (ASCP) > Cytology-Histology Supervisor > Carle Foundation Hospital > Laboratory and Pathology Services > 611 West Park Street > Urbana, Illinois 61801 > 217-383-3572 > sharon.davis-devine@carle.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 85, Issue 26 **************************************** ------------------------------ Message: 2 Date: 28 Dec 2010 16:10:21 -0500 From: Alisha Dynan Subject: [Histonet] Experienced Histotech and Histology Supervisor Jobs To: histonet@lists.utsouthwestern.edu Message-ID: <322850652.1293570621270.JavaMail.cfservice@SL4APP4> Content-Type: text/plain; charset="utf-8" Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 3 Date: Tue, 28 Dec 2010 21:51:53 +0000 From: Tony Henwood Subject: RE: [Histonet] Chuck Churukian To: "'Akemi Allison'" , Histonet Message-ID: <6D6BD1DE8A5571489398B392A38A71570358AB@xmdb02.nch.kids> Content-Type: text/plain; charset="us-ascii" What disturbing news. Hopefully Chuck can fight on and be back to full strength by April. All our prayers and support are for Chuck and his family. A father figure in Histochemistry. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Monday, 27 December 2010 12:12 PM To: Histonet Subject: [Histonet] Chuck Churukian I just heard from Irene Churukian today. Please put Chuck in your thoughts and prayers. Irene said this: CLL cells changed in character so Chuck began chemo treatments on Dec. 1. His second one was Dec. 21... He has six altogether - three weeks apart. The first cycle was difficult... he was very weak.... in bed most of the time. He is now improving ... thankfully. He has never been sick and confined to bed/home so he gets cabin fever easily. People have been so kind to visit him. So our Christmas this year was very different from years past. We are hoping and praying that by April, he will be in remission after all the treatments are completed. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 4 Date: Tue, 28 Dec 2010 15:22:54 -0800 (PST) From: Jonathan Krupp Subject: [Histonet] Fwd: Nor Cal Histotech program To: histonet@lists.utsouthwestern.edu Message-ID: <531815146.272564.1293578574576.JavaMail.root@mercury.deltacollege.edu> Content-Type: text/plain; charset=utf-8 Hi I am new here. I am part of an EM tech training program at Delta College in Stockton, CA. We train students to do EM, fixing, sectioning, staining, and instrument operation. Some of my students have been asking about LM histotech sort of things. They want to know more about histo techniques in general and maybe broadening their job prospects beyond EM. I am exploring the possibilities of adding more LM histotech things to the program and seek advice. Is there room for another program? Is seeking accreditation worth while? What would I need to get set up? Are there jobs available for graduates? Just getting started, your advice will be invaluable. Jon Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu ------------------------------ Message: 5 Date: Tue, 28 Dec 2010 19:48:02 -0500 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] Fwd: Nor Cal Histotech program To: "Jonathan Krupp" , Message-ID: <587BDAFD5B6A4379A22840FB4B8A03A0@HP2010> Content-Type: text/plain; format=flowed; charset="UTF-8"; reply-type=original There is SUCH a need for histotech programs. Currently, there are only 39 histologic technician (HT) (most community college based) and 4 histotechnologist (HTL) (need 4 year degree). And there are shortages everywhere. There are 2 HT programs in California. One in Berkley, one in Walnut. The accrediting agency is National Accrediting Agency for Clinical Laboratory Sciences (NAACLS). www.naacls.org For information on how to contact the two California programs, once on the NAACLS website, - on the left, click on Find a Program - Under Program Type, click on HT & hit Search. Programs are listed alphabetically by state. - If you want to talk with HTL program directors, follow above directions, except click on HTL. Any program director would be willing to give you more information. If you look under "accreditation" on the left, there is HT and HTL. Click on one, and you can find the accreditation standards you would have to follow. There are a few standards that are different between HT and HTL, so you need to look at both. The advantage of being an accredited HT or HTL program is that the students are eligible to take the national certification exam through American Society for Clinical Pathology (ASCP), immediately upon successful completion of the NAACLS accredited HT or HTL program. However, since you are just starting out, and look like you need lots of information, I'm going to suggest going to the National Society for Histotechnology (NSH) website. More information about what histotechs are, about meetings, state society presidents, jobs board, membership with journal specific for histology/tissue, etc. There is also a 3 hour continuing education you can purchase, titled "How to Start an Accredited HT or HTL Program in either a College or a Hospital". I presented it at the NSH Symposium in 2008, and it was recorded with the PowerPoint, so you can listen to me and look at the PowerPoint. It has lots of advantages and disadvantages of starting different types of programs, contacts, proof that we need more programs, etc. Granted, the information is 3 years old, and there are a lot more HT and HTL program than there were 3 years ago, but most of the other information is the same. Just a disclaimer, I don't make any money from this. I had presented it every year from 1999-2008, and NSH thought it might work as an on-line module, for people like yourself who need the information at times other than in the fall when the NSH symposium is. Go to www.nsh.org - Across the top, click on Professional Development - About the 5th one down, click on On-Line Learning Center - Click on Meeting Content - Scroll down and click on NSH 2008 Annual Symposium/Convention in Pittsburgh, PA - Click on #27 = How to Start an Accredited HT or HTL program Once you get some information under your belt, I'd be more than happy to answer questions specific for your situation. Just look on the NAACLS web page, under HT or HTL (we have both programs), and you'll find me in Michigan. :-) Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Jonathan Krupp" Sent: Tuesday, December 28, 2010 6:22 PM To: Subject: [Histonet] Fwd: Nor Cal Histotech program > > > > Hi > > I am new here. I am part of an EM tech training program at Delta College > in Stockton, CA. > > We train students to do EM, fixing, sectioning, staining, and instrument > operation. > > Some of my students have been asking about LM histotech sort of things. > They want to know more about histo techniques in general and maybe > broadening their job prospects beyond EM. > > I am exploring the possibilities of adding more LM histotech things to the > program and seek advice. Is there room for another program? Is seeking > accreditation worth while? What would I need to get set up? Are there jobs > available for graduates? > > Just getting started, your advice will be invaluable. > > Jon > > Jonathan Krupp > Delta College > 5151Pacific Ave. > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 28 Dec 2010 20:18:03 -0500 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] bouin solution To: "wassan alkadhumi" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original If the tissue is in Bouins, the excess Bouins needs to be washed out of the tissue immediately after fixation. Water could be used to rinse out the excess picric acid, but I think some people are worried that there could be some cell swelling if the tissue is left in the water too long (particularly if the tissue is underfixed/not stablized enough). If I remember correctly, too high of a percent of alcohol (95%, for example), will cause the picric acid salts to precipitate in the tissue, and it would be like trying to microtome tissue with sand in it. So 50-70% alcohol is a good compromise. Have enough water to remove the excess picric acid salts, but not too much water to cause tissue swelling, and not too much percent of alcohol to cause picric acid salts to precipitate in the tissue. For your slides already microtomed and on slides - these have already been fixed and processed. If the tissue was fixed in a picric acid fixative like Bouins, there is no need for the step of putting the slide in Bouins. If the tissue was fixed in something else (10% neutral buffered formalin, for example), then the slide/tissue needs to be placed into Bouins in order for the trichrome stains to work correctly. This is called either post-fixing or post-mordanting. There are several theories as to why the Bouins is needed for the trichromes (change tissue charges, change tissue density), but the end result is that Bouins fixed or Bouins post-mordanted tissue have muscles that are red and collagen that are blue or green (depending upon which dye you use). If the NBF fixed tissue is not post-mordanted, the red is a red/blue or red/green, and the collagen has strands of red in it. In other words, the colors are not as true or as crisp. Now, the slides that were post-mordanted in Bouins have tissue that is yellow. We need to get the yellow out. Not so much because of the picrate salts that precipitate, but more because the yellow color would interfere with the reds and the blues or greens colors that you are trying to make. The tissue is only 3-5 um thick, so it won't take as long to remove the color. And the tissue has been well fixed, dehydrated and cleared during tissue processing. So there are lots of cross-links to stabilize the tissue. So rinsing in water is only going to take a few minutes, and the tissue is stabilized, so there shouldn't be any cell size changes. You could rinse in 50-70% alcohol, but that's only 50-30% water, so it is going to take a lot longer to get rid of the yellow color than rinsing in water. Rinsing in tap water is fine for most labs, as long as the quality of tap water is good (not too acidic or basic, not too much iron or sulfur, no microorganisms). I like to rinse with d. water before any stain, just in case there is any contaminating metal that might interfere with the stains (we have a lot of iron in our part of the state). By the way, putting the slides in Bouins in a coplin jar with a lid screwed on, then putting in a 60 degree C. oven or water bath for 45-60 minutes works great. This would reduce your turn around time by 17 hours! Two words of caution. 1) Don't breath in the hot Bouins fumes (picric acid, acetic acid, formaldehyde). Open up the coplin jar in a hood. 2) Don't stick the hot coplin jar into cold running water. Coplin jar will break/crack. We usually take the slides out of the hot Bouins, and put the slides into a new coplin jar with water, then put this under the running water. The lid is put back on the Bouins coplin jar, and allowed to cool. Some places reuse the Bouins for a number of times. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "wassan alkadhumi" Sent: Tuesday, December 28, 2010 9:49 AM To: Subject: [Histonet] bouin solution > Dear Histonet > I have question concerning bouin solution, we use it in masson trichrome > before > starting the stain. we incubate the slides trimmed at 3 micron in bouin > solution > for 18 hr in room temp which is not controlled. Then we wash the slides > using > running water first and then distilled water, but according to what > i red, when using bouin solution,the tissue should be washed with (50-70)% > alcohol to prevent deterioration of staining in time. now i should mention > that > in the our masson trichrome procedure there is no washing with alcohol > but only > with water. > > what should i do? In the book its mentioned that we have to wash with > alcohol if > the tissue is not processed yet, is that why the alcohol step is missing > in > masson tri procedure?and why will the stain deteriorate if some of the > bouin > solution stayed in the tissue? > The main fixative that is used in our lab for all grooses and biopsy is > 10% > formalin (except bone marrow biopsy). bouin solution is used after > processing of > the tissue and deparaffinization of the sections is finished. > Thanks > > Wassan > Histotechnician > Shorh hospital > North of Iraq > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 29 Dec 2010 05:10:01 -0800 (PST) From: Kim Tournear Subject: [Histonet] Florida State Histology License To: Histonet Message-ID: <313964.32140.qm@web120203.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Can any one tell me how to go about getting a Florida State Histology License? And I understand that there is a test to take for the Supervisor's license as well....I'll take info for that too,,,,thanks in advance!! ~Kim~? ? OU ROCKS!!!! ~Don't be afraid your life will end, be afraid it will never begin~ ------------------------------ Message: 8 Date: Wed, 29 Dec 2010 09:12:50 -0500 From: "Houston, Ronald" Subject: [Histonet] CAP question for pediatric pathology labs To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" CYP.00190 Phase I N/A YES NO For laboratories that perform non-gynecologic cytopathology, does the laboratory participate in a peer educational program in NON-GYNECOLOGIC cytopathology (e.g., CAP Interlaboratory Comparison Program in Non-Gynecologic Cytopathology NGC)? How are labs responding to this request for a peer-educational program in non-gyn cytopath, as there is no such program for pediatric cytopathology, and, in the opinion of our pathologists, the CAP program is useless as far as it pertains to pediatrics? We have had varying responses from inspectors which we have adopted and then the next inspector says this isn't good enough and you should be doing something else. There has been little to no direction from CAP. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 9 Date: Wed, 29 Dec 2010 19:23:28 +0500 (PKT) From: tahseen@brain.net.pk Subject: [Histonet] AgNORs staining prtocol & UVC lamp safeguard To: Histonet@lists.utsouthwestern.edu Message-ID: <25574.203.135.35.66.1293632608.squirrel@brain.net.pk> Content-Type: text/plain;charset=iso-8859-1 Dear All, 1 Would you like to share your AgNORs staining prtocol with me? 2 We are using Leica CM1850 US Cryostat, we are going to use UVC lamp for disinfection, so what kind of safeguard are recommended other then sliding window has been properly closed. Thanks advance Muhammad Tahseen Senior Supervisore Histopathology SKMCH & RC Lahore Pakistan. ------------------------------ Message: 10 Date: Wed, 29 Dec 2010 10:42:56 -0500 From: Subject: RE: [Histonet] Florida State Histology License To: "'Kim Tournear'" , "'Histonet'" Message-ID: <002a01cba76f$17e1bef0$47a53cd0$@imagesbyhopper.com> Content-Type: text/plain; charset="us-ascii" Kim, You will have to go to the Florida Department of Health website to get the application. For a supervisor license, you will need to meet specific requirements and then take an additional 24 hours of CEUs and pay the license fee. Please click on the following link for more information. http://www.doh.state.fl.us/mqa/ClinLab/index.html There are a number of links on that page, you might wish to begin with the Applicant Information link. Good Luck! Michelle From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Wednesday, December 29, 2010 8:10 AM To: Histonet Subject: [Histonet] Florida State Histology License Can any one tell me how to go about getting a Florida State Histology License? And I understand that there is a test to take for the Supervisor's license as well....I'll take info for that too,,,,thanks in advance!! ~Kim~ OU ROCKS!!!! ~Don't be afraid your life will end, be afraid it will never begin~ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1191 / Virus Database: 1435/3346 - Release Date: 12/29/10 ------------------------------ Message: 11 Date: Wed, 29 Dec 2010 12:29:23 -0500 From: "Hutton, Allison" Subject: [Histonet] billing for breast cases To: Message-ID: <38A56C4F4630D348A50B3720409270870E0FE294@dhmail.dhorg.org> Content-Type: text/plain; charset="iso-8859-1" A debate has arisen and I am looking to histonet for a more definitive answer. We have a breast surgeon who, after he removes the lumpectomy specimen, always goes back and removes more tissue around the margins of the lumpectomy. Our question is how should these additional margins be charged. Currently we charge an 88305 for each of the additional margins (there are no sutures indicating any orientation, however, one can determine the old and new margins). Is 88305 the correct charge in this situation or should they be higher at an 88307? Thank you in advance, Allison ------------------------------ Message: 12 Date: Wed, 29 Dec 2010 12:54:16 -0500 From: DKBoyd@chs.net Subject: Re: [Histonet] billing for breast cases To: "Hutton, Allison" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" My understanding is: Inked margins (on any specimen type) are 88307. Doesn't specify who inks the margin. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Hutton, Allison" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/29/2010 12:35 PM To cc Subject [Histonet] billing for breast cases A debate has arisen and I am looking to histonet for a more definitive answer. We have a breast surgeon who, after he removes the lumpectomy specimen, always goes back and removes more tissue around the margins of the lumpectomy. Our question is how should these additional margins be charged. Currently we charge an 88305 for each of the additional margins (there are no sutures indicating any orientation, however, one can determine the old and new margins). Is 88305 the correct charge in this situation or should they be higher at an 88307? Thank you in advance, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 85, Issue 27 **************************************** CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. From jaylundgren <@t> gmail.com Wed Dec 29 14:31:52 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Dec 29 14:31:57 2010 Subject: [Histonet] Re: if you could pick your own floor surface... In-Reply-To: References: <0908FC0A43B87A4FB60EDCCA06AABC246696A9@exchange.cmc-nh.org> Message-ID: SHAG CARPETING FTW!!!!111 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Wed Dec 29 16:22:57 2010 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Dec 29 16:23:01 2010 Subject: [Histonet] RRAS Message-ID: Hello all!! Hope everyone is gearing up for the festivities of this weekend with great excitement!!! Got a quick question. We are going to be using the RRAS antibody and I'm trying to figure out what a good positive control tissue is. I looked on the abcam site and it says colon, spleen, heart, CA lines. I don't know if it means colon cancer, etc. or if those three tissues and then any cancer line of any tissue would also work. I currently only have breast and prostate cancer tissues. I do also have normal heart, colon, and spleen. Does anyone have an answer for this? Thanks in advance, my fellow histo. hotties!!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From kathyenelson <@t> hotmail.com Wed Dec 29 16:55:09 2010 From: kathyenelson <@t> hotmail.com (Kathy Nelson) Date: Wed Dec 29 16:55:13 2010 Subject: [Histonet] Cracking in tissue esp. BCC Message-ID: Any suggestion in parallel cracking in tissues would be helpful. THANKS From Lori.Disher <@t> HCAhealthcare.com Thu Dec 30 10:22:45 2010 From: Lori.Disher <@t> HCAhealthcare.com (Lori.Disher@HCAhealthcare.com) Date: Thu Dec 30 10:22:52 2010 Subject: [Histonet] Florida fees to upgrade from Technician to Technologist Message-ID: <778DD853CF606049A37FC2059C8BA07A7551AD4D78@FWDCWPMSGCMS04.hca.corpad.net> There are several of us at my workplace that are upgrading from technician to technologist. One of these ladies told me that the Florida DOH said that the fee was $203.00. On the State application it looks like it is only $100.00. Every time I call the DOH I get transferred to someone's voice mail and no return phone calls. Is there someone out there that knows for sure what the fee is? I will probably just send the $100.00, but I didn't want my application to have a glitch. Happy New Year to you all Lori A Disher Fawcett Memorial Hospital Port Charlotte, FL 33952 lori.disher@hcahealthcare.com From Lori.Disher <@t> HCAhealthcare.com Thu Dec 30 11:35:18 2010 From: Lori.Disher <@t> HCAhealthcare.com (Lori.Disher@HCAhealthcare.com) Date: Thu Dec 30 11:35:24 2010 Subject: [Histonet] Florida fees to upgrade from Histology Technician to Technologist Message-ID: <778DD853CF606049A37FC2059C8BA07A7551AD4ECD@FWDCWPMSGCMS04.hca.corpad.net> There are several of us at my workplace that are upgrading from technician to technologist. One of these ladies told me that the Florida DOH said that the fee was $203.00. On the State application it looks like it is only $100.00. Every time I call the DOH I get transferred to someone's voice mail and no return phone calls. Is there someone out there that knows for sure what the fee is? I will probably just send the $100.00, but I didn't want my application to have a glitch. Happy New Year to you all Lori A Disher Fawcett Memorial Hospital Port Charlotte, FL 33952 lori.disher@hcahealthcare.com From HParker <@t> Skaggs.Net Thu Dec 30 12:18:06 2010 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Thu Dec 30 12:17:31 2010 Subject: [Histonet] Sorry about my last post In-Reply-To: <45044880-6562-4f3c-8fab-6496cd055cc4@email1.skaggs.net> References: <45044880-6562-4f3c-8fab-6496cd055cc4@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F506004700E68B1@email1.skaggs.net> I did not mean to resend the whole digest back. I forget to erase. Helayne CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. From quinntl <@t> umkc.edu Thu Dec 30 13:30:00 2010 From: quinntl <@t> umkc.edu (Quinn, Tim L.) Date: Thu Dec 30 13:30:09 2010 Subject: [Histonet] methylene blue stained tissue Message-ID: <8E26873FB7498E44997E28F5F5EB62F332B2145A7D@UM-EMAIL06.um.umsystem.edu> Hello Histies, I received rodent dermis that has been stained with methylene blue. I would like to perform immunolabeling experiments on this tissue. Should I expect any interference from the methylene blue stain? Tim Quinn Office of Research Administration MEDLAB Senior Research Technician/Central Lab Manager University of Missouri at Kansas City School of Medicine Room M3-202 2411 Holmes Street Kansas City, Missouri 64108-2792 Phone- 816-235-1990 or 816-235-1904 or FAX- 816-235-6517 From kathyenelson <@t> hotmail.com Thu Dec 30 13:40:37 2010 From: kathyenelson <@t> hotmail.com (Kathy Nelson) Date: Thu Dec 30 13:40:41 2010 Subject: [Histonet] Cracks in tissue Message-ID: Solutions for cracks in tissue microscopically esp. in tumors and BCC specimen. Thanks From adam.smith <@t> adeccona.com Thu Dec 30 16:41:42 2010 From: adam.smith <@t> adeccona.com (Smith, Adam) Date: Thu Dec 30 16:41:56 2010 Subject: [Histonet] I would like to post this mesage on to all subscribers on histonet Message-ID: <0980CC29978E784588667491783476B80A54E7E9AB@MEUSITINFEVS02.am.adecco.net> Job opening for a Histology Manager in Augusta, GA 30901 Hello, my name is Adam, I am a recruiter at Adecco Medical & Science and I have an opening for a Histology Manager in Augusta, GA 30901. This position will fill quickly so if you are interested please reach out to me as soon as you can. I have listed the job description and required qualifications about the position below. If you are not interested please feel free to pass this email along to anyone who may like to apply for this position. I look forward to speaking with you Please send me 2 - 3 professional references and an updated resume Job Details: Position title: Histology Manager Start date: ASAP Duration: Direct Hire Location: Augusta, GA 30901 Hours: 1st shift Pay: 28.00 max Job Details Reports to the Director of Laboratory Operations and is responsible for coordinating and directing activities of assigned section and subordinates engaged in performing routine and non-routine histology procedures. In doing so, ensures quality and quantity standards relating to the delivery of laboratory services are achieved by assuring results. -Knowledge of Science and Mathematics normally acquired through completion of an Associate's Degree. -Active/current certification as a Histotechnologist (HT or HTL) by the Board of Registry of the American Society of Clinical Pathologists is required. -Five years experience as an HTIII to include 2 years of management experience or 3-5 years experience as an HTIII and completion of internal manager training program, or equivalent work experience in a pathology lab setting. SUMMARY OF PURPOSE: Reports to the Laboratory Manager and is responsible for coordinating and directing activities of assigned section and subordinates engaged in performing routine and non-routine histology procedures. In doing so, ensures quality and quantity standards relating to the delivery of laboratory services are achieved by assuring results. ESSENTIAL FUNCTIONS: 1. In collaboration with the Laboratory Manager plans, coordinates, monitors and oversees work of assigned section and subordinate personnel engaged in performing histology procedures needed to obtain data for disease diagnosis and treatment. 2. Accomplishes, or effectively recommends the following: * Prepares schedules to assure adequate coverage. * Assigns work * Counsels employees on work or working relationships * Arranges and/or conducts job training, continuing education. * Approves time worked * Completes employee orientation for assigned section. * Interviews, hires, and evaluates the performance of and, when necessary, disciplines and discharges subordinate supervisory personnel. * Approves hiring and, when necessary, discharge recommendations of subordinate personnel and assists in resolving complex employee relations matters. 3. Ensures all technical procedures are performed correctly. Procedures with higher priority are completed first, equipment has proper maintenance performed and kept in good working order. 4. Reviews and evaluates new products, equipment. 5. Evaluates and implements new procedures as necessary. 6. Makes supply decisions with sufficient accuracy so no supplies run out or remains beyond the expiration date. 7. Responsible for daily quality control and quality assurance to include establishing training and maintaining Q.C. and Q.A protocol with subordinate staff. 8. Coordinates monthly QA and QC data. 9. Ensures that laboratory section meets all safety and other regulatory requirements. 10. Effectively manages technical aspects of hospital accounts 11. Gathers information and carries through to completion assigned projects while adhering to established time frames. 12. Prepares periodic reports as necessary. 13. Assures activities of assigned section are coordinated to insure quality patient care and economics of operation. 14. Participates in continuing education as required. 15. Consults with LIS Administrator to troubleshoot LIS problems and enhancements. 16. Participates in the development of assigned section and department wide policies and procedures. 17. Maintains all section records relating to operation such as quality control and productivity statistics, incident reports and the like. 18. Reviews and summarizes records and prepares reports for management review. 19. Develops and maintains cooperative working relationships with physicians and various other employees. 20. Provides advice and direction to subordinates for technical problems encountered. Explains and demonstrates appropriate techniques or methods as necessary. 21. Performs routine procedures in achieving workload demands. 22. Maintains knowledge of current trends and developments in the field of expertise. 23. Performs other duties as assigned POSITION REQUIREMENTS: Education and formal training: Knowledge of Science and Mathematics normally acquired through completion of an Associate's Degree. License: Active/current certification as a Histotechnologist (HT or HTL) by the Board of Registry of the American Society of Clinical Pathologists is required. Experience: 1. Five years experience as an HTIII to include 2 years of management experience or 3-5 years experience as an HTIII and completion of internal manager training program, or equivalent work experience in a pathology lab setting. 2. Analytical skills necessary in order to participate in planning of department activities provide input into purchase of equipment, and so forth. 3. Interpersonal skills necessary in order to coordinate activities of various units within the department, exchange information with vendors, and so forth. 4. Must be able to communicate effectively by telephone, e-mail and in person with customers, co-workers and supervisors. 5. Ability to apply concepts such as fractions, percentages, ratios, and proportions to practical situations. 6. Relies on experience and judgment to plan and accomplish goals. 7. A certain degree of creativity and latitude is expected. 8. Requires ability to handle irate customers, good judgment on tough decisions, and ability to resolve complex situations PHYSICAL/MENTAL REQUIREMENTS: 1. Occasional walking, standing, bending and reaching when filing, photocopying, etc. 2. Light lifting of under 50 pounds on an infrequent basis. 3. There is a moderate potential for skin, eye, mucous membrane, non-intact skin or parenteral contact with potentially infectious materials or chemicals may result from the performance of an employee's regular duties. 4. Repetitive wrist motion when performing microtomy procedures. If interested please contact Adam Smith Medical & Science recruiting specialist Adecco Medical & Science 2235 South Clinton Avenue Rochester, NY 14618 Office: 585.454.5511 Toll Free: 866.217.3454 Cell: 585.414.1869 Fax: 585.454.5519 adam.smith@adeccona.com Visit us @ www.adeccousa.com | www.ajilonconsulting.com | Apply Here Today! From jshelley <@t> sanfordburnham.org Fri Dec 31 15:56:56 2010 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Fri Dec 31 15:57:03 2010 Subject: [Histonet] RE: RRAS In-Reply-To: References: Message-ID: Hi Sarah, I have used this antibody and just plain intestine is fine. If you worked with CD31 before it looks a lot like that. Hope this helps. HistoLand have a great New Year!!! Kind Regards! ? John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, December 29, 2010 5:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RRAS Hello all!! Hope everyone is gearing up for the festivities of this weekend with great excitement!!! Got a quick question. We are going to be using the RRAS antibody and I'm trying to figure out what a good positive control tissue is. I looked on the abcam site and it says colon, spleen, heart, CA lines. I don't know if it means colon cancer, etc. or if those three tissues and then any cancer line of any tissue would also work. I currently only have breast and prostate cancer tissues. I do also have normal heart, colon, and spleen. Does anyone have an answer for this? Thanks in advance, my fellow histo. hotties!!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laura.Miller <@t> leica-microsystems.com Fri Dec 31 16:02:09 2010 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Fri Dec 31 16:02:16 2010 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 12/31/2010 and will not return until 01/04/2011. I will be out of the office until 1-04-11. I will respond to your email when I return. Happy New Year!!! Have a safe and merry holiday season!!! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________