From louise.renton <@t> gmail.com Mon Aug 2 02:25:07 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Mon Aug 2 02:25:22 2010 Subject: [Histonet] QC on stained slides Message-ID: Hi all As part of a self assessment programme conducted by my employer, and related to my performance review and salary adjustment, I need to determine the criteria of what makes a stained slide acceptable or unacceptable. I was wondering if anyone out there had a "checklist" that they would be willing to share, that i could perhaps adapt. I realise that the easiest would be to send slides out for external control, but in this case it is not feasible. What I put together is this: - Quality of decalcification, processing, infiltration - Quality of sections (no wrinkles, missing bits, scores etc) - Entire representation of tissue area - staining pattern as expected according to protocol - coverslipped without bubbles or other inclusions - labelled neatly and correctly but, the question inmy mind is what would be the criteria that would make a slide merely adequate or truely outstanding? PLease help thank you -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Dorothy.L.Webb <@t> HealthPartners.Com Mon Aug 2 09:14:35 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Aug 2 09:14:41 2010 Subject: [Histonet] decalcification Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094767@HPEMX3.HealthPartners.int> I would appreciate any feedback on what all are using in your decalcification process. We get a lot of large bones in and the past 2-3 months have noticed a huge problem in our microtomy process with these samples. We have been grossing the bones in and leaving the sample in the cassette in 10% formalin for 24 hours befoere placing in decal for up to 8 hours and still having the inner portion of the sample look underprocessed and crunchy! Any suggestions would be appreciated! Dorothy Webb, HT Regions Hospital Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From sgoebel <@t> xbiotech.com Mon Aug 2 10:09:49 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Aug 2 10:09:52 2010 Subject: [Histonet] decalcification Message-ID: <20100802080949.9e2d9aa830e8449a2412eb1e4f2f067e.0e97fba773.wbe@email04.secureserver.net> I have used formical for years and it works great. You don' to pre-fix your samples because there is formalin in with the decal. It fixes and decals at the same time. I don't know how many sp ecimens you have, but a sure fire way to check is to weigh the bone after g solution. coming out. As out. Now it is just huge bones, I use this works like a charm! H PS-The formical is made by Dec Sarah Goebel, B.A., HT (ASCP) Histotechnician < XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] decalcification From: "Webb, Dorothy L" <[1]Dorothy.L.Webb@HealthPartners.Com> Date: Mon, August 02, 2010 7:14 am To: "'[2]histonet@lists.u <[3]histonet@lists.uts I would appreciate any feedback on what all are using in your decalcificati 2-3 months have no these samples. We have sample in the cassette in 10% fo in decal for up to 8 hours and still ha sample look underprocessed and crunchy! Any appreciated! Dorothy Webb, HT Regions Hospital Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are inte are addres individual responsible fo recipient, please be advised that y error and that any use, dissemination, forw copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the He You will be rei us. HealthPartners R001. _______________________________________________ Histonet mailing list [4]Histonet@lists.utsouth [5]http: References 1. 3D"mailto:Dorothy.L.Webb@HealthPartners 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:Histonet@lists.utsouthwestern.edu" 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Margaret.Perry <@t> sdstate.edu Mon Aug 2 10:49:15 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Aug 2 10:49:21 2010 Subject: [Histonet] RE: Histonet Digest, Vol 81, Issue 1 In-Reply-To: <20100801170042.9843C64EAB8@barracuda.sdstate.edu> References: <20100801170042.9843C64EAB8@barracuda.sdstate.edu> Message-ID: What antibody are you trying to use? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, August 01, 2010 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Help with Biocare polymer detection kit!! (Jennifer Campbell) ---------------------------------------------------------------------- Message: 1 Date: Sat, 31 Jul 2010 12:03:39 -0700 From: "Jennifer Campbell" Subject: [Histonet] Help with Biocare polymer detection kit!! To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FF1@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="iso-8859-1" I have been trying to get the Mach 1 polymer kit from Biocare to work on my K9 and feline tissue and I am getting terrible nonspecific staining in my positive and negative controls. It appears to be sticking to something in the glands of the intestinal epithelia. I am also getting nonspecific staining in my LN tissue. I decided to switch over to polymer detection kit because I had been dealing with the issue of endogenous biotin when using the streptavidin biotin-based detection system, and now I am getting this other nonspecific staining! I have tried doing runs eliminating my protein block and eliminating the negative control and primary antibody step altogether (to see if maybe the protein block or the diluent used in my negative control and antibody dilutions are causing the problem). I have also reduced the incubation time of both the probe and polymer from 15 min and 30 min, respectively, to 10 min each. I have tried cutting down my antigen retrieval time substantially as well. I'm not sure what else could be causing this. Any suggestions?? Thanks in advance, Jennifer Campbell ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 1 *************************************** From rjbuesa <@t> yahoo.com Mon Aug 2 10:55:21 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 2 10:55:25 2010 Subject: [Histonet] decalcification In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094767@HPEMX3.HealthPartners.int> Message-ID: <298258.11054.qm@web65702.mail.ac4.yahoo.com> You cannot set a fixed time for decalcification. You have to leave the bone in the decalcifying solution until iy is soft and ready for processing. Ren? J. --- On Mon, 8/2/10, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] decalcification To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, August 2, 2010, 10:14 AM I would appreciate any feedback on what all are using in your decalcification process.? We get a lot of large bones in and the past 2-3 months have noticed a huge problem in our microtomy process with these samples.? We have been grossing the bones in and leaving the sample in the cassette in 10% formalin for 24 hours befoere placing in decal for up to 8 hours and still having the inner portion of the sample look underprocessed and crunchy!? Any suggestions would be appreciated! Dorothy Webb, HT Regions Hospital Technical Supervisor 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Mon Aug 2 11:09:36 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Aug 2 11:09:43 2010 Subject: [Histonet] Irving Ht Position Message-ID: <6F33D8418806044682A391273399860F04ABF6AA@s-irv-ex301.PathologyPartners.intranet> Nueterra, a leading developer and manager of physician-owned surgery centers and specialty hospitals has an immediate opportunity available for a Histotechnician. Great opportunity for a Histotechnician in a brand new laboratory! Nueterra Pathology Services in Irving, TX is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers a competitive salary, medical/dental insurance, 401K plan, and vacation/sick leave. Interested applicants should contact Meredith Hale phone 214-596-2219 or through email at mhale@carisdx.com . Meredith Hale HT (ASCP) Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From smcbride <@t> andrew.cmu.edu Mon Aug 2 12:19:46 2010 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Mon Aug 2 12:20:07 2010 Subject: [Histonet] decalcification In-Reply-To: <298258.11054.qm@web65702.mail.ac4.yahoo.com> References: <298258.11054.qm@web65702.mail.ac4.yahoo.com> Message-ID: <4C0E32D7-98F9-435D-A743-16E05D8AECE2@andrew.cmu.edu> > Hi Dorothy, I'm not sure what size bone specimens with which you are working, but I generally work with femurs & calvaria from rabbits, canine & other small rodents. I fix my specimens longer in 10% NBF using an automated vacuum infiltration processor. Following fixation, if decal is required, I will decal in buffered formic acid solution and use a calcium reagent set to titrate to the decalcification endpoint. If you would like any specifics, contact me offline. Best regards, ~Sean McBride Senior Researcher Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-9807 (fax) smcbride@andrew.cmu.edu > > --- On Mon, 8/2/10, Webb, Dorothy L wrote: > > > From: Webb, Dorothy L > Subject: [Histonet] decalcification > To: "'histonet@lists.utsouthwestern.edu'" > Date: Monday, August 2, 2010, 10:14 AM > > > I would appreciate any feedback on what all are using in your decalcification process. We get a lot of large bones in and the past 2-3 months have noticed a huge problem in our microtomy process with these samples. We have been grossing the bones in and leaving the sample in the cassette in 10% formalin for 24 hours befoere placing in decal for up to 8 hours and still having the inner portion of the sample look underprocessed and crunchy! Any suggestions would be appreciated! > > Dorothy Webb, HT > Regions Hospital Technical Supervisor > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jreichensperger <@t> siumed.edu Mon Aug 2 12:29:57 2010 From: jreichensperger <@t> siumed.edu (Joel Reichensperger) Date: Mon Aug 2 12:30:02 2010 Subject: [Histonet] Collagen Type II antibody Message-ID: <4C570095.7040707@siumed.edu> I am in need of a good collagen Type II antibody that could be used in both rat and human samples. We would prefer a polyclonal antibody but might settle for a monoclonal. Anyone have any good suggestions? We will be staining the samples with DAB. Thanks in advance. Joel Reichensperger -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) From liz <@t> premierlab.com Mon Aug 2 12:35:01 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Aug 2 12:34:23 2010 Subject: [Histonet] Collagen Type II antibody In-Reply-To: <4C570095.7040707@siumed.edu> Message-ID: Joel We use an antibody we purchase from MD Biosciences - its quite expensive but we find it works really well for us on multiple species with chondroitinase digestion. Protocol: Rabbit anti Human Collagen Type II Clone: Polyclonal Vendor: MD Biosciences Catalog Number: MD20211 Species: Rabbit Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger Sent: Monday, August 02, 2010 11:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Collagen Type II antibody I am in need of a good collagen Type II antibody that could be used in both rat and human samples. We would prefer a polyclonal antibody but might settle for a monoclonal. Anyone have any good suggestions? We will be staining the samples with DAB. Thanks in advance. Joel Reichensperger -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Montina.VanMeter <@t> pbrc.edu Mon Aug 2 13:57:00 2010 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Mon Aug 2 13:57:34 2010 Subject: [Histonet] RE: Histonet Digest, Vol 81, Issue 1 In-Reply-To: References: <20100801170042.9843C64EAB8@barracuda.sdstate.edu> Message-ID: <4FE7FB862E90E448AE32388E759220E5789D6F@pbrcas31.pbrc.edu> Jennifer, I don't use the Mach 1 for animal tissue. I am working with rat tissue and use the Biocare Rabbit on Rodent or Mouse on Rat HRP or AP Polymers. I have absolutely no background and my sections are 40um thick. Perhaps you are using the wrong kit. Hope this helps, Tina -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Monday, August 02, 2010 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 81, Issue 1 What antibody are you trying to use? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, August 01, 2010 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Help with Biocare polymer detection kit!! (Jennifer Campbell) ---------------------------------------------------------------------- Message: 1 Date: Sat, 31 Jul 2010 12:03:39 -0700 From: "Jennifer Campbell" Subject: [Histonet] Help with Biocare polymer detection kit!! To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FF1@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="iso-8859-1" I have been trying to get the Mach 1 polymer kit from Biocare to work on my K9 and feline tissue and I am getting terrible nonspecific staining in my positive and negative controls. It appears to be sticking to something in the glands of the intestinal epithelia. I am also getting nonspecific staining in my LN tissue. I decided to switch over to polymer detection kit because I had been dealing with the issue of endogenous biotin when using the streptavidin biotin-based detection system, and now I am getting this other nonspecific staining! I have tried doing runs eliminating my protein block and eliminating the negative control and primary antibody step altogether (to see if maybe the protein block or the diluent used in my negative control and antibody dilutions are causing the problem). I have also reduced the incubation time of both the probe and polymer from 15 min and 30 min, respectively, to 10 min each. I have tried cutting down my antigen retrieval time substantially as well. I'm not sure what else could be causing this. Any suggestions?? Thanks in advance, Jennifer Campbell ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 1 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jorgelhg <@t> yahoo.com Mon Aug 2 14:17:48 2010 From: jorgelhg <@t> yahoo.com (Jorge Luis Hurtado) Date: Mon Aug 2 14:17:52 2010 Subject: [Histonet] Beetles Head sectioning Message-ID: <963399.46550.qm@web35407.mail.mud.yahoo.com> Hi all: Does anyone has?the proper procedures to obtain cryostat and/or paraffin sections of beetles head?. I have not been succesful at obtaining one. My samples cut well but during collection from the cryotome it tends to curl so I tend to loose?the brain tissue. Additionally, is there any place that I can send my beetles head samples to be sectioned? This is to do basic brain measurements. I would really appreciate all possible feedback and pointing me to the right references if possible. Thanks Jorge L. Hurtado From roqroq2000 <@t> yahoo.com Mon Aug 2 14:19:39 2010 From: roqroq2000 <@t> yahoo.com (Sahar Darwish) Date: Mon Aug 2 14:19:43 2010 Subject: [Histonet] (no subject) Message-ID: <832468.52832.qm@web56701.mail.re3.yahoo.com> hi there ? i need to know?in a detail? a special stain ?to demonstrate epilepsy in rat brain ? thanks a lot From saby_joseph_a <@t> yahoo.com Mon Aug 2 16:49:51 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Mon Aug 2 16:49:55 2010 Subject: [Histonet] decalcification In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094767@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094767@HPEMX3.HealthPartners.int> Message-ID: <851434.66778.qm@web114406.mail.gq1.yahoo.com> Dorothy- If your bones are indeed large, 24 hours in 10% NBF will not be sufficient for fixation.? If the fixation isn't sufficient, none of the processes following that will be as effective as they might be.? Even after trimming, I would recommend fixing an additional 24-48 hours for every mm of thickness of your bone section, at a minimum.? If you are in a hospital setting and need reults quickly, you can use a processor (with heat, pressure and vacuum) to speed up the fixation. I use buffered formic acid to decal.? The final concentration of formic acid is about 20-25%, so it is rather aggressive, but it also allows you to read the bone marrow after decal.? If you need results in a short time (hospital setting again) you can use an aggressive reagent like RDO for decal, but?keep in mind that nuclear detail will be lacking, but bone structure?should still be readable.? If you do not have the reagents to determine decal endpoints, you can (with training/practice) use a needle to pass through the sections to help determine if decal is complete.? The needle should move through the specimen smoothly and not get hung up and stiff in the middle of the section.? Flexibility of the bone section can tell you when you are appoaching the time when the needle test will give you useable results. Please do not read this as an endorsement for RDO or any other super aggressive decal solution.? Personally, I always insist on being able to read all tissue elements, and these solutions, although fast, give less than optimal staining results. If you have further questions, please ask me off line. Joe Saby, BA HT ________________________________ From: "Webb, Dorothy L" To: "histonet@lists.utsouthwestern.edu" Sent: Mon, August 2, 2010 10:14:35 AM Subject: [Histonet] decalcification I would appreciate any feedback on what all are using in your decalcification process.? We get a lot of large bones in and the past 2-3 months have noticed a huge problem in our microtomy process with these samples.? We have been grossing the bones in and leaving the sample in the cassette in 10% formalin for 24 hours befoere placing in decal for up to 8 hours and still having the inner portion of the sample look underprocessed and crunchy!? Any suggestions would be appreciated! Dorothy Webb, HT Regions Hospital Technical Supervisor 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Mon Aug 2 17:26:06 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Aug 2 17:26:10 2010 Subject: [Histonet] Yuba City HT Position Message-ID: <6F33D8418806044682A391273399860F04B46D1C@s-irv-ex301.PathologyPartners.intranet> Histology Lab Tech. (Yuba City, CA) Licensed with a minimum of 3 years experience. Must be able to work with little supervision. Competitive salary and benefits. Please email resume' to rwood@northvalleygi.com for immediate consideration. * Location: Msvl/Yuba City * Compensation: DOE Meredith Hale HT (ASCP) Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From annigyg <@t> gmail.com Tue Aug 3 01:27:01 2010 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Tue Aug 3 01:27:04 2010 Subject: [Histonet] tissue arrays Message-ID: Hi everyone I am looking for tissue array slides for testing my ER/PR/her2 - to purchase online I know we could make them ourselves but we are a small lab and do not have the numbers of cases to do so our first 'real' CAP inspection is looming who is using what and why? please advise thanks AnnieinArabia -- Anne van Binsbergen (Hope) Abu Dhabi UAE From louise.renton <@t> gmail.com Tue Aug 3 02:25:35 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Aug 3 02:25:52 2010 Subject: [Histonet] QC on stained slides In-Reply-To: References: Message-ID: Thank you for your very helpful suggestions....The situation here is a little weird as I am the only one doing the sectioning in this unit (research unit), and mostly i look at my own slides to do histomorphometry...so I have to grade myself??!!! Oh well, we do the best we can best regards On Mon, Aug 2, 2010 at 7:05 PM, WILLIAM DESALVO wrote: > Attached is a copy of the QA sheet that I provided at one of my NSH > presentations. Review and use as needed. > > I believe your question of what makes a slide adequate or superior may be > the wrong question. As long as you have a subjective review with a wide and > varied specification, it will be extremely difficult to set a scoring > process that will provide the desired feed back. I suggest you might want to > approach in a different way and look at the number of defect or unacceptable > products produced as compared to an agreed upon and high standard. > > use a Six Sigma tool to help you. I suggest you need an opportunities for > improvement procedure and use the Defects per Million Opportunities > (DPMO) tool. This will provide a process to evaluate the performance above > and below standard in a simple and efficient manner. Your goal is never to > deliver adequate work to your customer, the pathologist, but to always > deliver the highest quality of work. You cannot improve the quality of work > produced unless you know what is not meeting standard, not what is adequate > or superior. Whether it is one person or multiple people working in the lab, > there will always be variation in the product delivered because it is a > manual process. You always want to reduce and narrow that variation to > maintain the highest and consistent quality. But to narrow the variation you > must have a standard and the standard must be agreed upon by the persons > producing and the persons reviewing the work. > > I believe using a Six Sigma tool will provide you with the feedback you > require and help you maintain the highest quality of slides delivered to the > pathologists. Standardize your procedures and protocols, develop standards > (highest quality standards) w/ your pathologists and then document, review, > track and trend defects to improve the process. The data collected will give > you specific information as to how you have performed to standard combined > with the specific number of units produced by you (quality and quantity > combined). This process will also allow you to compare multiple individuals > working in the department and compare those individuals to each > other. Everyone will be evaluated according to standards set for the work > produced, plain, simple and effective. > > *William DeSalvo,* B.*S., HTL(ASCP)* > * > * > > > > > Date: Mon, 2 Aug 2010 09:25:07 +0200 > > From: louise.renton@gmail.com > > > To: Histonet@lists.utsouthwestern.edu > > CC: > > Subject: [Histonet] QC on stained slides > > > > Hi all > > > > As part of a self assessment programme conducted by my employer, and > related > > to my performance review and salary adjustment, I need to determine the > > criteria of what makes a stained slide acceptable or unacceptable. I was > > wondering if anyone out there had a "checklist" that they would be > willing > > to share, that i could perhaps adapt. I realise that the easiest would be > > to send slides out for external control, but in this case it is not > > feasible. > > > > What I put together is this: > > > > - Quality of decalcification, processing, infiltration > > - Quality of sections (no wrinkles, missing bits, scores etc) > > - Entire representation of tissue area > > - staining pattern as expected according to protocol > > - coverslipped without bubbles or other inclusions > > - labelled neatly and correctly > > > > but, the question inmy mind is what would be the criteria that would make > a > > slide merely adequate or truely outstanding? > > > > PLease help > > > > thank you > > > > -- > > Louise Renton > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > +27 11 717 2298 (tel & fax) > > 073 5574456 (emergencies only) > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Kim.Donadio <@t> bhcpns.org Tue Aug 3 11:23:56 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Aug 3 11:24:25 2010 Subject: [Histonet] QC on stained slides In-Reply-To: Message-ID: Since you are the only one there it might be useful for you and your work place to do "proficiency testing". You can purchase one through CAP. The one that would measure the quality of your sections and stains is called HistoQIP. I don't have the link handy but I am sure you could find it on the CAP web site. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 louise renton Sent by: histonet-bounces@lists.utsouthwestern.edu 08/03/2010 02:25 AM To WILLIAM DESALVO cc Histonet@lists.utsouthwestern.edu Subject Re: [Histonet] QC on stained slides Thank you for your very helpful suggestions....The situation here is a little weird as I am the only one doing the sectioning in this unit (research unit), and mostly i look at my own slides to do histomorphometry...so I have to grade myself??!!! Oh well, we do the best we can best regards On Mon, Aug 2, 2010 at 7:05 PM, WILLIAM DESALVO wrote: > Attached is a copy of the QA sheet that I provided at one of my NSH > presentations. Review and use as needed. > > I believe your question of what makes a slide adequate or superior may be > the wrong question. As long as you have a subjective review with a wide and > varied specification, it will be extremely difficult to set a scoring > process that will provide the desired feed back. I suggest you might want to > approach in a different way and look at the number of defect or unacceptable > products produced as compared to an agreed upon and high standard. > > use a Six Sigma tool to help you. I suggest you need an opportunities for > improvement procedure and use the Defects per Million Opportunities > (DPMO) tool. This will provide a process to evaluate the performance above > and below standard in a simple and efficient manner. Your goal is never to > deliver adequate work to your customer, the pathologist, but to always > deliver the highest quality of work. You cannot improve the quality of work > produced unless you know what is not meeting standard, not what is adequate > or superior. Whether it is one person or multiple people working in the lab, > there will always be variation in the product delivered because it is a > manual process. You always want to reduce and narrow that variation to > maintain the highest and consistent quality. But to narrow the variation you > must have a standard and the standard must be agreed upon by the persons > producing and the persons reviewing the work. > > I believe using a Six Sigma tool will provide you with the feedback you > require and help you maintain the highest quality of slides delivered to the > pathologists. Standardize your procedures and protocols, develop standards > (highest quality standards) w/ your pathologists and then document, review, > track and trend defects to improve the process. The data collected will give > you specific information as to how you have performed to standard combined > with the specific number of units produced by you (quality and quantity > combined). This process will also allow you to compare multiple individuals > working in the department and compare those individuals to each > other. Everyone will be evaluated according to standards set for the work > produced, plain, simple and effective. > > *William DeSalvo,* B.*S., HTL(ASCP)* > * > * > > > > > Date: Mon, 2 Aug 2010 09:25:07 +0200 > > From: louise.renton@gmail.com > > > To: Histonet@lists.utsouthwestern.edu > > CC: > > Subject: [Histonet] QC on stained slides > > > > Hi all > > > > As part of a self assessment programme conducted by my employer, and > related > > to my performance review and salary adjustment, I need to determine the > > criteria of what makes a stained slide acceptable or unacceptable. I was > > wondering if anyone out there had a "checklist" that they would be > willing > > to share, that i could perhaps adapt. I realise that the easiest would be > > to send slides out for external control, but in this case it is not > > feasible. > > > > What I put together is this: > > > > - Quality of decalcification, processing, infiltration > > - Quality of sections (no wrinkles, missing bits, scores etc) > > - Entire representation of tissue area > > - staining pattern as expected according to protocol > > - coverslipped without bubbles or other inclusions > > - labelled neatly and correctly > > > > but, the question inmy mind is what would be the criteria that would make > a > > slide merely adequate or truely outstanding? > > > > PLease help > > > > thank you > > > > -- > > Louise Renton > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > +27 11 717 2298 (tel & fax) > > 073 5574456 (emergencies only) > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From cparsons <@t> vt.edu Tue Aug 3 11:50:47 2010 From: cparsons <@t> vt.edu (Parsons, Catherine) Date: Tue Aug 3 11:50:50 2010 Subject: [Histonet] New Cryostat Reviews Message-ID: The small university department that I work for is hoping to purchasing a cryostat of our own, after several bouts of having to beg for use of an ancient unit in another building. Money for large equipment purchases is pretty scarce these days, so I want to make sure that we get a reliable instrument. I have information on Sakura's Cryo3, Leica's CM1950, and Microm's (Thermo Scientific now) HM 525 and 550. I was hoping for some opinions about the units from these vendors, or suggestions for additional models. We are a group of small research labs, so would not have need for motorized sectioning or the really high end models with all the bells and whistles. We would just like a reliable cryostat of our own! Thank you for your comments. Cathy Parsons From brian <@t> prometheushealthcare.com Tue Aug 3 12:11:15 2010 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Tue Aug 3 12:11:20 2010 Subject: [Histonet] New Histology Opening in Memphis, TN Message-ID: <013901cb332e$e1d843b0$a588cb10$@com> New Opening with a practice dedicated solely to the practice of gastrointestinal and liver pathology Position is in Memphis, TN Day shift Monday thru Friday either 8am to 5pm or 7am to 4pm Lead experience preferred Please let me know if you may be interested Thanks! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From istillma <@t> bidmc.harvard.edu Tue Aug 3 12:40:52 2010 From: istillma <@t> bidmc.harvard.edu (istillma@bidmc.harvard.edu) Date: Tue Aug 3 12:40:43 2010 Subject: [Histonet] (no subject) Message-ID: <4488e546-93a5-4c13-b075-d999720c3a24@blur> Sent via DROID on Verizon Wireless From grudow1 <@t> jhmi.edu Tue Aug 3 12:41:57 2010 From: grudow1 <@t> jhmi.edu (Gay Rudow) Date: Tue Aug 3 12:42:28 2010 Subject: [Histonet] unsubscribe Message-ID: <864A73846CE8F04D995603351682C29C9AB3AB6015@JHEMTEXVS2.win.ad.jhu.edu> From pathology.histology <@t> gmail.com Tue Aug 3 13:06:36 2010 From: pathology.histology <@t> gmail.com (path lab) Date: Tue Aug 3 13:06:52 2010 Subject: [Histonet] Hospitalities Message-ID: Has anyone heard if there is going to be any hospitalities at the convention in Seattle this year? We haven't seen anything listed on the NSH home page yet. Thanks in advance, Paul From Rhonda.Hartz <@t> saskatoonhealthregion.ca Tue Aug 3 12:57:05 2010 From: Rhonda.Hartz <@t> saskatoonhealthregion.ca (Hartz, Rhonda SktnHR) Date: Tue Aug 3 13:14:01 2010 Subject: [Histonet] Gram control Message-ID: Hi. To all of you who suggested using a Slim Jim for a gram control - We do not have Slim Jim's available here, so we tried similar products. We also had a colleague purchase a couple of Slim Jims for us while she was on holidays in California. Our gram control only showed gram positive cocci (beautiful and abundant), but what do you do for the gram negatives? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.hartz@saskatoonhealthregion.ca From arvidsonkristen <@t> yahoo.com Tue Aug 3 13:25:03 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Aug 3 13:25:07 2010 Subject: [Histonet] Recycled Formalin Message-ID: <706190.81798.qm@web65712.mail.ac4.yahoo.com> Has anyone had any quality issues (or any issues) when using recycled formalin? ? Thanks. From BSullivan <@t> shorememorial.org Tue Aug 3 13:37:51 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Tue Aug 3 13:40:23 2010 Subject: [Histonet] Recycled Formalin In-Reply-To: <706190.81798.qm@web65712.mail.ac4.yahoo.com> Message-ID: I have tried to recycle both Alcohol and Xylene with not very good results. I'm not sure what kind of quality you would get on recycled formalin. You first need to know what, if anything, is being removed from the material. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 kristen arvidson To Sent by: histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Recycled Formalin 08/03/2010 02:25 PM Has anyone had any quality issues (or any issues) when using recycled formalin? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mchelle252002 <@t> yahoo.com Tue Aug 3 13:41:03 2010 From: mchelle252002 <@t> yahoo.com (Michelle Mathews) Date: Tue Aug 3 13:41:06 2010 Subject: [Histonet] New Cryostat Reviews Message-ID: <191899.59718.qm@web53301.mail.re2.yahoo.com> Cathy, I would certainly go with the Leica model - their cryostats are very reliable, with smooth motion and easy adjustments and cleaning. I have cut frozens on a few other brands, and far preferred the Leica; the angle is simple to adjust, the temps are simple to read and set, and the decontamination procedure is very easy. Good luck! Michelle Mathews, HT(ASCP) Translational Pathology Laboratory UNC- Chapel Hill From alyssa <@t> alliedsearchpartners.com Tue Aug 3 14:19:09 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Aug 3 14:19:16 2010 Subject: [Histonet] Histology Positions in FL (Permanent and Full Time) Message-ID: Below are two histology positions open in Florida. Please let me know if you or anyone you know would be interested. :-) The first one is a MOHS histology position and the second is a regular histotechnician/histotechnologist position. *The first position:* ** MPath Search Partners, A division of Allied Search Partners is currently looking for histology candidates in the Sarasota, FL area. Position: Moh?s Histotechnician/Histotechnologist Requirements: HT/HTL preferred but not required - Moh?s Histology Experience i.e. experience working with Mohs Tissue - 1-5 years experience - The lab processes Frozen Sections and Permanent Section specimens, need someone experienced who can come in ready to work Environment: Day Shift, Monday-Friday, about 8am-5pm Physician Office Lab To apply: Please submit resume to Alyssa@alliedsearchpartners.com to be considered for a phone interview with one of our recruiters. Your resume is always kept confidential until we have your consent. Visit our website to refer a friend for a generous bonus! www.alliedsearchpartners.com *The second position:* Our client, located in Lakeland, FL has retained MPath Search Partners to assist in the search for a qualified Histotechnician/Histotechnologist. This is a unique opportunity to join a financially strong and growing organization in a key role within the company. Shift: Full Time/Permanent, 3am-11am Requirements: Florida Licensed Technician or Technologist ASCP certification Preferred Duties: - Routine Histology - Help with moving out of our old laboratory and into our new larger laboratory - Help with the new histology laboratory set up To apply: Please submit resume to alyssa@alliedsearchpartners.com Benefits: ? Three health plans to choose from - All through Blue Cross/Blue Shield,(Plan # 3462),(Plan # 3464),(Plan # 3800). ? 50% coverage for employee on Health and Dental Plan ? Long Term Disability ? 10 vacation/sick days (5 vacation days, 5 sick days the first year of employment) -- Alyssa Peterson, Director of Candidate Recruitment Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From mtitford <@t> aol.com Tue Aug 3 15:04:51 2010 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Tue Aug 3 15:05:06 2010 Subject: [Histonet] Formalin substitutes, anyone? Message-ID: <8CD015E4471B627-1518-1FF3@Webmail-d112.sysops.aol.com> I am curious!! In a Histonet message last week, Tony Henwood mentioned the formalin substitutes "UMfix", "Kryofix" (now called Neo-fix I think) and "Spuitfix". 1) How many surgical pathology labs use formalin substitutes for routine work? 2) Which ones do they use? (which is the most popular?) 3) Do they use formalin substitutes just for initial fixation, or on their tissue processors as well? I would be interested in hearing. Yes, I know, curiosity killed the cat!! Thank you to anyone who replies. Histotechnology is endlessly fascinating. Michael Titford Pathology USA Mobile AL USA = From sprice2003 <@t> gmail.com Tue Aug 3 15:17:58 2010 From: sprice2003 <@t> gmail.com (Sally Price) Date: Tue Aug 3 15:18:02 2010 Subject: [Histonet] RE: Help with Biocare polymer detection kit!! Message-ID: Jennifer: There are a variety possible causes for the background staining, which probably have more to do with the specimen you're attempting to stain than the detection systems, especially because you stated that you observed similar background with a biotin/avidin-based system. The first thing that comes to mind is whether or not Mach 1 is intended for use on canine/feline tissue; I suspect that its not, but you should check with Biocare's tech support to be sure. My guess is that either the immunologic enhancer reagent or antibody conjugated to the polymer-enzyme reagent is binding to endogenous immunolglobulins. Another possible cause of the background is that your tissue is over- retrieved/digested. It could also be that your antibody is not diluted adequately, or, as you suggested, the protein-blocking reagent is actually contributing to the problem. Good Luck! Sally ---------------------------------------------------------------------- Message: 1 Date: Sat, 31 Jul 2010 12:03:39 -0700 From: "Jennifer Campbell" Subject: [Histonet] Help with Biocare polymer detection kit!! To: histonet@lists.utsouthwestern.edu I have been trying to get the Mach 1 polymer kit from Biocare to work on my K9 and feline tissue and I am getting terrible nonspecific staining in my positive and negative controls. It appears to be sticking to something in the glands of the intestinal epithelia. I am also getting nonspecific staining in my LN tissue. I decided to switch over to polymer detection kit because I had been dealing with the issue of endogenous biotin when using the streptavidin biotin-based detection system, and now I am getting this other nonspecific staining! I have tried doing runs eliminating my protein block and eliminating the negative control and primary antibody step altogether (to see if maybe the protein block or the diluent used in my negative control and antibody dilutions are causing the problem). I have also reduced the incubation time of both the probe and polymer from 15 min and 30 min, respectively, to 10 min each. I have tried cutting down my antigen retrieval time substantially as well. I'm not sure what else could be causing this. Any suggestions?? Thanks in advance, Jennifer Campbell From amosbrooks <@t> gmail.com Tue Aug 3 18:02:33 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Aug 3 18:02:39 2010 Subject: [Histonet] Beetles Head sectioning Message-ID: Hi, It's always great to try this kind of stuff and see what we can do. But when it comes down to really needing to get it done right and have it looking REALLY good, there is no better place to send it than to Damien Laudier. He is truly talented histologist, entomologist and artist. Take a look at his website and you 'll see what I mean. http://www.laudierhistology.com/ Have a great evening, Amos Message: 5 Date: Mon, 2 Aug 2010 12:17:48 -0700 (PDT) From: Jorge Luis Hurtado Subject: [Histonet] Beetles Head sectioning To: histonet@lists.utsouthwestern.edu Message-ID: <963399.46550.qm@web35407.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all: Does anyone has the proper procedures to obtain cryostat and/or paraffin sections of beetles head?. I have not been succesful at obtaining one. My samples cut well but during collection from the cryotome it tends to curl so I tend to loose the brain tissue. Additionally, is there any place that I can send my beetles head samples to be sectioned? This is to do basic brain measurements. I would really appreciate all possible feedback and pointing me to the right references if possible. Thanks Jorge L. Hurtado From AnthonyH <@t> chw.edu.au Tue Aug 3 18:58:20 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Aug 3 18:58:52 2010 Subject: [Histonet] Formalin substitutes, anyone? In-Reply-To: <8CD015E4471B627-1518-1FF3@Webmail-d112.sysops.aol.com> Message-ID: Hi Michael No we do not use formalin substitutes Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA I am curious!! In a Histonet message last week, Tony Henwood mentioned the formalin substitutes "UMfix", "Kryofix" (now called Neo-fix I think) and "Spuitfix". 1) How many surgical pathology labs use formalin substitutes for routine work? 2) Which ones do they use? (which is the most popular?) 3) Do they use formalin substitutes just for initial fixation, or on their tissue processors as well? I would be interested in hearing. Yes, I know, curiosity killed the cat!! Thank you to anyone who replies. Histotechnology is endlessly fascinating. Michael Titford Pathology USA Mobile AL USA = _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From BenatM <@t> gosh.nhs.uk Wed Aug 4 05:13:47 2010 From: BenatM <@t> gosh.nhs.uk (Malika Benatti) Date: Wed Aug 4 05:14:36 2010 Subject: [Histonet] Olig2 (ab33427) on the bond max Message-ID: <4C594B82.4626.0038.0@gosh.nhs.uk> ** Proprietary ** ** Reply Requested When Convenient ** I was wondering if anyone on the histonet list is using abCam Olig2 (ab33427) on the bond max. I have been trying various titration and enzyme treatment on surgical brain tissue with not much success al we seams to achieve is nuclear staining and heavy background staining. Any suggestions would be appreciated! Thanks in advance Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* From Ronald.Houston <@t> nationwidechildrens.org Wed Aug 4 08:07:11 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Aug 4 08:07:20 2010 Subject: [Histonet] Olig2 (ab33427) on the bond max In-Reply-To: <4C594B82.4626.0038.0@gosh.nhs.uk> References: <4C594B82.4626.0038.0@gosh.nhs.uk> Message-ID: I had problems with this one too. It now seems that this particular antibody has been removed from AbCam's product range due to some QC problems. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malika Benatti Sent: Wednesday, August 04, 2010 6:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Olig2 (ab33427) on the bond max ** Proprietary ** ** Reply Requested When Convenient ** I was wondering if anyone on the histonet list is using abCam Olig2 (ab33427) on the bond max. I have been trying various titration and enzyme treatment on surgical brain tissue with not much success al we seams to achieve is nuclear staining and heavy background staining. Any suggestions would be appreciated! Thanks in advance Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From gp62 <@t> georgetown.edu Wed Aug 4 11:21:15 2010 From: gp62 <@t> georgetown.edu (Guillermo Palchik) Date: Wed Aug 4 11:21:21 2010 Subject: [Histonet] TUNEL of frozen thick rat brain slices. Message-ID: <2172E9C6-C7F0-4034-B556-769FB6A1AFEC@georgetown.edu> Dear Histologists, Has anyone done TUNEL on 40 um flash-frozen rat brain sections? I have tried tweaking the protocol (we use the Millipore Apoptag kit - S7101) from our ongoing 20 um slices by increasing the incubation times from 1 hour to 2 and 4 hours but we still get poor staining. Also, could I do overnight incubations? the tissue is flash frozen, but it does get fixed at the beginning of the protocol with PFA (4%) and subsequently with acetic acid-EtOH. Thanks, Gil -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 From Montina.VanMeter <@t> pbrc.edu Wed Aug 4 12:18:03 2010 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Wed Aug 4 12:43:06 2010 Subject: [Histonet] TUNEL of frozen thick rat brain slices. In-Reply-To: <2172E9C6-C7F0-4034-B556-769FB6A1AFEC@georgetown.edu> References: <2172E9C6-C7F0-4034-B556-769FB6A1AFEC@georgetown.edu> Message-ID: <4FE7FB862E90E448AE32388E759220E5789D76@pbrcas31.pbrc.edu> Gil, I routinely incubate free-floating 40um rat brain sections overnight or longer @ 4 degrees centigrade according to the particular antibody that I am working with. Our sections are perfused in 4% paraformaldehyde and cryoprotected with 30% sucrose prior to sectioning. How long are you fixing the tissue prior to staining? Tina -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Guillermo Palchik Sent: Wednesday, August 04, 2010 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TUNEL of frozen thick rat brain slices. Dear Histologists, Has anyone done TUNEL on 40 um flash-frozen rat brain sections? I have tried tweaking the protocol (we use the Millipore Apoptag kit - S7101) from our ongoing 20 um slices by increasing the incubation times from 1 hour to 2 and 4 hours but we still get poor staining. Also, could I do overnight incubations? the tissue is flash frozen, but it does get fixed at the beginning of the protocol with PFA (4%) and subsequently with acetic acid-EtOH. Thanks, Gil -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Wed Aug 4 12:57:02 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Aug 4 12:57:13 2010 Subject: [Histonet] Flat Mount Message-ID: <20100804105702.9e2d9aa830e8449a2412eb1e4f2f067e.93d96942cb.wbe@email04.secureserver.net> Hello all, I am being asked to do immunostaining on fla eyes. First, what is a flat mount and does an on how to make one? Second, what is the methodol stains on these? Do you have to fix them, cut them, done this and need some help. Thanks guys!! Sarah Goebel, B.A., HT (ASCP) Histotechnician [DEL: XBiot 8201 East Riverside Dr. Austin, Texas (512)386-5107 From gayle.callis <@t> bresnan.net Wed Aug 4 14:19:25 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Aug 4 14:19:58 2010 Subject: [Histonet] Re: Decalcification Message-ID: <001a01cb3409$f767b4f0$e6371ed0$@callis@bresnan.net> Dorothy Webb wrote: I would appreciate any feedback on what all are using in your decalcification process. We get a lot of large bones in and the past 2-3 months have noticed a huge problem in our microtomy process with these samples. We have been grossing the bones in and leaving the sample in the cassette in 10% formalin for 24 hours befoere placing in decal for up to 8 hours and still having the inner portion of the sample look underprocessed and crunchy! Any suggestions would be appreciated! ********************* Dorothy, Large bones need to fix longer than 24 hours unless you cut bone into smaller slabs or representative samples e.g. size that fits into a cassette. If the samples are NOT cut open or slabbed to allow good fixation as soon as you receive in the lab, you will have to still allow time for fixation. Any residual pinkness/reddish color means unfixed. Whole bones take a long time to fix in NBF, so bisect, open, cut slabs. Decalcification can be done with buffered formic acid, ranging from commercial 4.5% formic concentration up to 20% formic acid. Bones must be TOTALLY FIXED and must be to be protected from effects of acid decalcification, then decalcification can proceed. We simply fix longer and then decalcify in 10% to 15% aqueous formic acid, do the weight loss/weight gain endpoint determination for bone sectioning without problems. The other problem is processing and what is probably happening is the bone is actually under processed, and you need to use a longer processing schedule. That is difficult in a hospital setting, so make sure the samples are smaller if your processing shortened. Also, poor dehydration, clearing and most important - paraffin infiltration. Opaque appearance and poor microtomy are indicators of 1) poor fixation 2) incomplete decalcification 3)poor dehydration/clearing and paraffin infiltration or ALL of these. You can solve the poor paraffin infiltration by leaving the bone in paraffin longer, preferably with under a vacuum. We have a heated vacuum oven to do this, but usually have automated bone processing schedules that are longer than a routine schedules for soft tissues. The latter may not be feasible for you in your hospital. I also suggest you look into the commercially available kit for endpoint determination from Poly Sciences IF you don't want to make up the solutions for this in house. This method is commonly found in histotechnology text books but I will send the protocol privately IF you want it. The weight loss/weight gain method is what we use now since we no longer have an xray machine, aka FAXITRON. The chemical test is accurate. You could use a rapid decalcifying solution (HCl, commerical solutions are fine, just read the MSDS to know the acid concentration), but use the endpoint test - that will save you a lot of grief by preventing overexposure to acid ("overdecalcification"). Here is a very quick decalcification endpoint test as long as you have a balance that weighs in milligrams. This is a published method and excellent for EDTA decalcifcation since there is NO good chemical test for EDTA. If you don't have a balance, then do the chemical endpoint test. WEIGHT LOSS/WEIGHT GAIN ENDPOINT TEST ADVANTAGE: One can decalcify many samples in one container, fast, efficient DISADVANTAGES: Requires a balance that reads in milligrams. Specimen must be blotted free of water, for accurate weighing each time you weigh the sample. Technic: 1.Rinse NBF off bone, blot with paper towel, WEIGH BONE and RECORD BEGINNING WEIGHT. Suspend bone in acid or EDTA decalcifier, agitate. Large bones can be started at end of day, sit overnight in decalcifier. Hydrochloric acid decalcifiers are very fast, formic acid is slower/gentler. 2.After 4 to 5 hours in strong acid or overnight in formic acid or EDTA, remove bone, rinse with water, BLOT, weigh. RECORD WEIGHT. If bone shows loss of weight, change decalcifying solution, resuspend, and repeat as many times as necessary. EDTA should be changed but not as often as acid. 3.When bone begins to GAIN WEIGHT, the bone is decalcified. Once calcium is removed, water is taken on and the weight increases. 4.Rinse bone with running tap water for a several hours, and process. For EDTA, one can suspend bones and check every day for accuracy but bones can be left in the EDTA over a weekend. Acid decalcified bones cannot be left over a weekend, remove from acid, put in NBF, then resume decalcification on next working day. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From sraibley <@t> yahoo.com Wed Aug 4 20:29:01 2010 From: sraibley <@t> yahoo.com (Susan Raibley) Date: Wed Aug 4 20:29:06 2010 Subject: [Histonet] Re: Recycled Formalin Message-ID: <802441.21399.qm@web56006.mail.re3.yahoo.com> We recycle formalin, xylene and alcohol and they all turn out very nicely. We use the CBG Biotech formalin and solvent recycler and then assay/test each batch and return the formalin back to 10% NBF by adding either distilled water or formaldehyde depending on what end it came out on and then add the buffer back by following the directions for how much is needed based on volume. What issues are you having? Do you assay each batch to see what percent you have back and then bring back to neutral? Susan Bincsik, HT (ASCP) ? Has anyone had any quality issues (or any issues) when using recycled formalin? ? Thanks. From Ceri.Allen <@t> nottingham.ac.uk Thu Aug 5 06:00:29 2010 From: Ceri.Allen <@t> nottingham.ac.uk (Ceri Allen) Date: Thu Aug 5 06:01:29 2010 Subject: [Histonet] Dewaxing problem Message-ID: <5B721D295F00644C9E1D87C420DB775003A590EF@VUIEXCHC.ad.nottingham.ac.uk> Dear Histonetters, I've recently started having a problem with tissue sections stubbornly refusing to de-wax. I've been using 2 x 5 minutes in Histoclear to dewax before re-hydrating and staining with normal H&E. Until about three weeks ago this process worked fine. Leaving the sections in the Histoclear for longer seems to have little effect and using Xylene instead hasn't done much either - it's taking up to two days or more. The type of tissue seems to be irrelevant, the sections are all cut at 5 microns, and I haven't changed any part of the processing. I've used fresh alcohols and solvents, clean glassware - everything I can think of. Can anyone give me any more ideas or possible causes? because i'm stumped L Thanks Ceri Allen Research Technician School of Veterinary Medicine and Science Sutton Bonnington Campus University of Nottingham Tel. 0115 95 16459 ceri.allen@nottingham.ac.uk This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation. From rjbuesa <@t> yahoo.com Thu Aug 5 07:52:12 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 5 07:52:16 2010 Subject: [Histonet] Dewaxing problem In-Reply-To: <5B721D295F00644C9E1D87C420DB775003A590EF@VUIEXCHC.ad.nottingham.ac.uk> Message-ID: <542494.80730.qm@web65714.mail.ac4.yahoo.com> Use a 2%aqueousus solution odish washingng soap at 90?c (3 changes of 1 minute each) and your problems wildisappearar.

Ren? J. --- On Thu, 8/5/10, Ceri Allen wrote: From: Ceri Allen Subject: [Histonet] Dewaxing problem To: histonet@lists.utsouthwestern.edu Date: Thursday, August 5, 2010, 7:00 AM Dear Histonetters, I've recently started having a problem with tissue sections stubbornly refusing to de-wax. I've been using? 2 x 5 minutes in Histoclear to dewax before re-hydrating and staining with normal H&E. Until about three weeks ago this process worked fine. Leaving the sections in the Histoclear for longer seems to have little effect and using Xylene instead hasn't done much either - it's taking up to two days or more. The type of tissue seems to be irrelevant, the sections are all cut at 5 microns, and I haven't changed any part of the processing. I've used fresh alcohols and solvents, clean glassware - everything I can think of. Can anyone give me any more ideas or possible causes? because i'm stumped? L Thanks Ceri Allen Research Technician School of Veterinary Medicine and Science Sutton Bonnington Campus University of Nottingham Tel. 0115 95 16459 ceri.allen@nottingham.ac.uk This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwhite <@t> mcleodhealth.org Thu Aug 5 08:08:12 2010 From: mwhite <@t> mcleodhealth.org (mwhite@mcleodhealth.org) Date: Thu Aug 5 08:08:11 2010 Subject: [Histonet] searching for pathology dictation system Message-ID: We still use dictaphones for gross dictation. Does anyone have a system you can recommend? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 From lblazek <@t> digestivespecialists.com Thu Aug 5 08:31:51 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Aug 5 08:31:56 2010 Subject: [Histonet] searching for pathology dictation system In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E390EAF72C6B5@IBMB7Exchange.digestivespecialists.com> We use Start Stop with out much problem. www.startstop.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mwhite@mcleodhealth.org Sent: Thursday, August 05, 2010 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] searching for pathology dictation system We still use dictaphones for gross dictation. Does anyone have a system you can recommend? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Thu Aug 5 08:42:18 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Aug 5 08:42:21 2010 Subject: [Histonet] searching for pathology dictation system In-Reply-To: References: Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B75749@exchange.cmc-nh.org> Melanie, We are using a voice recognition system that interfaces with our LIS. We are using Dragon with a Voice Brook interface. Voice Brook is a pathology specific system that really enhances and picks up on the most challenging medical terminology. We use this for Gross, Pathologist Dictation and Autopsy and have had a great deal of success with it. The PA and Pathologist edit their own dictation as they go along so that when they are finished the report is ready to be finagled. Everything is standardized and template driven. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mwhite@mcleodhealth.org Sent: Thursday, August 05, 2010 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] searching for pathology dictation system We still use dictaphones for gross dictation. Does anyone have a system you can recommend? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Aug 5 08:55:22 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Aug 5 08:55:28 2010 Subject: [Histonet] best myoepithelial cell marker for salivary gland Message-ID: <8C023B4AB999614BA4791BAEB26E2738399F36@UWHC-MAIL01.uwhis.hosp.wisc.edu> Hi 'netters, One of our pathologists has asked me to find out (in my spare time!) what the best myoepithelial cell marker is for salivary gland. Any opinions? Thanks. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From alisha <@t> ka-recruiting.com Thu Aug 5 10:18:11 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Aug 5 10:17:49 2010 Subject: [Histonet] GM of Pathology Ops Job Opportunity Message-ID: <948135597.1281021490937.JavaMail.cfservice@sl4app2> Dear Histonet Subscribers, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few histotechnician, histotechnologist, and cytotechnologist openings across the country. Our clients typically assist with relocation expenses. One particular client I am working with is looking for a General Manager of Anatomic Pathology Operations for an anatomic pathology lab in NJ. This laboratory is known for its cutting edge technology, LIS system, and its automation. They are looking for someone with 10-20+ years experience in histology/pathology, preferably an HTL(ASCP) certification, at least 10+ years experience in management/operations, and someone who is a strong leader with excellent communication skills. This is a high level pathology position and offers an excellent compensation and benefits package. If interested, please email me your resume to alisha@ka-recruiting.com. Below is a list of some of the opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current HT and HTL Opportunities: 1. Histology Manager - MI 2. Histology Supervisor - NV 3. Histology Supervisor (must have HTL) - Atlanta, GA 4. Histology Supervisor - Central GA 5. Histotech - NYC, NY 6. Grossing Tech - NYC, NY 7. Histotech - IN 8. Histotech - SC 9. Histotech - MA 10. Pathologist's Assistant - NV 11. Pathologist's Assistant - Maine If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From adam <@t> sensorhealth.com Thu Aug 5 10:26:39 2010 From: adam <@t> sensorhealth.com (Adam Harris) Date: Thu Aug 5 10:26:49 2010 Subject: [Histonet] Microscope Slide stamp Message-ID: Hi Jill, I know this was a while ago but if you are still in need of something to help label your slides we are carrying an Ultra Fine Lab Marker that works really well on the frosted ends of slides. It is resistant to alcohol and xylene, and writes very nicely on smaller areas. Feel free to check us out at www.sensorhealth.com for more details and let us know if you would like to try a free sample. Adam Harris Sales Associate Sensor Health Inc. 110-6 Turnbull Crt. Cambridge, ON N1T 1K6 T: 1-888-777-7080 T: 519-621-1515 F: 519-621-8778 www.sensorhealth.com From Rcartun <@t> harthosp.org Thu Aug 5 11:06:37 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Aug 5 11:06:47 2010 Subject: [Histonet] Autopsy charge Message-ID: <4C5AA94D.7400.0077.1@harthosp.org> If you do an autopsy on a patient who has died at home (with no connection to your medical institution) at the request of the family, what do you charge? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From lloyd.3 <@t> osu.edu Thu Aug 5 11:51:48 2010 From: lloyd.3 <@t> osu.edu (Mary Lloyd) Date: Thu Aug 5 11:51:53 2010 Subject: [Histonet] PEN SLIDES FOR LASER CAPTURE Message-ID: <04A71EFE05FE5F4DAA05D0500FD0597002A0161E@Distal.dentnet.dent.ohio-state.edu> Does anyone have any experience with PEN membrane slides from Zeiss for laser capture microdissection. I have been working with one of our residents and we are having problems with the tissue staying on these slides. We could sure use help. Thanks Mary Lloyd From gentras <@t> auburn.edu Thu Aug 5 12:13:27 2010 From: gentras <@t> auburn.edu (Atoska Gentry) Date: Thu Aug 5 12:13:47 2010 Subject: [Histonet] RE: Microm HM 505 E Cryostat Message-ID: <4C5AAAE7.C676.0026.0@auburn.edu> hello, has anyone with this particular cryostat experienced frost build up on the top interior area located above the microtome? I've also noticed the temperature has for the past couple of weeks been reading ~ 3 - 4 C colder than temperature setting. Thus far it seems to still be sectioning just fine. I'm just curious to know if this is an indication of a malfunction of some sort? I also need to mention that on Monday morning I also observed quite a bit of water on the floor of the lab where this instrument is housed and it's source has yet to be determined. Any pointers will be much appreciated. From BSullivan <@t> shorememorial.org Thu Aug 5 12:21:27 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Thu Aug 5 12:23:56 2010 Subject: [Histonet] Autopsy charge In-Reply-To: <4C5AA94D.7400.0077.1@harthosp.org> Message-ID: The charge is usually given to the family of the deceased. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Richard Cartun" To Sent by: "Histonet" histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Autopsy charge 08/05/2010 12:06 PM If you do an autopsy on a patient who has died at home (with no connection to your medical institution) at the request of the family, what do you charge? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> cvmc.org Thu Aug 5 14:04:43 2010 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Thu Aug 5 14:04:48 2010 Subject: [Histonet] Autopsy charge In-Reply-To: <4C5AA94D.7400.0077.1@harthosp.org> Message-ID: For "outside" cases, we charge $1200 for full autopsy, including brain, $950 for full autopsy not including brain and $700 for a partial autopsy (chest only, abdomen only, brain only). Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, August 05, 2010 12:07 PM To: Histonet Subject: [Histonet] Autopsy charge If you do an autopsy on a patient who has died at home (with no connection to your medical institution) at the request of the family, what do you charge? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Elliott <@t> hli.ubc.ca Thu Aug 5 15:29:54 2010 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Thu Aug 5 15:30:32 2010 Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 37 In-Reply-To: <5C5F35C4.364@mail.mrl.ubc.ca> References: <5C5F35C4.364@mail.mrl.ubc.ca> Message-ID: <4C5ABCD2020000D60004D4FA@mail.mrl.ubc.ca> Hi Mighnon Did you get a response to your request. I may be able to send you some. Please contact me at mark.elliott(at)hli.ubc.ca Mark Date: Fri, 30 Jul 2010 15:25:00 -0500 From: Mighnon Lashus Subject: [Histonet] Adenovirus controls To: "histonet@lists.utsouthwestern.edu" Message-ID: <197CD0B02A81F94994A285C59C8AE05C05CE72A328@pgnexchange.pathgroup.com> Content-Type: text/plain; charset="us-ascii" I am looking for adenovirus controls, would anyone know where I could get some? I have checked Newcomer and ThermoFisher and they are out. Thanks, Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From dw18 <@t> uchicago.edu Thu Aug 5 15:45:30 2010 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Thu Aug 5 15:45:37 2010 Subject: [Histonet] Re: floating section incubation times Message-ID: <20100805154530.CHY83424@m4500-03.uchicago.edu> Hi Histonet, Tina & Gil I work on the same material as Tina and just wanted to echo her "overnight" comments with the addition that I routinely incubate 40um floating sections over the whole weekend at room temp (on a very slow shaker, 20 rpm, and with azide to stop bug growth. It all washes out before the peroxidase reaction.) I seem to remember from Fick's Law that the time taken to diffuse to the same endpoint concentration goes up with the square of the thickness, so if you double the thickness you would need 4x the incubation time - or else more concentrated antibody. The same distinction applies to "floating" vs on-slide incubations. {It wasn't clear to me if Gil's sections were floating or not). Since reagents can penetrate from both sides of a floating section, a section stained "on-slide" is effectively twice as thick and needs 4x the time. Note that the same principle applies to leaching out unbound reagents - you should lengthen your wash steps too, by the same factor, to prevent high background. happy staining -David David A. Wright, PhD University of Chicago Section of Neurosurgery ---- Original message ---- Date: Thu, 05 Aug 2010 12:07:03 -0500 (CDT) Subject: Histonet Digest, Vol 81, Issue 5 Message: 1 Date: Wed, 4 Aug 2010 12:18:03 -0500 From: "Montina Van Meter" Subject: RE: [Histonet] TUNEL of frozen thick rat brain slices. Gil, I routinely incubate free-floating 40um rat brain sections overnight or longer @ 4 degrees centigrade according to the particular antibody that I am working with. Our sections are perfused in 4% paraformaldehyde and cryoprotected with 30% sucrose prior to sectioning. How long are you fixing the tissue prior to staining? Tina >-----Original Message----- On Behalf Of Guillermo Palchik Sent: Wednesday, August 04, 2010 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TUNEL of frozen thick rat brain slices. Dear Histologists, Has anyone done TUNEL on 40 um flash-frozen rat brain sections? I have tried tweaking the protocol (we use the Millipore Apoptag kit - S7101)from our ongoing 20 um slices by increasing the incubation times from 1 hour to 2 and 4 hours but we still get poor staining. Also, could I do overnight incubations? the tissue is flash frozen, but it does get fixed at the beginning of the protocol with PFA (4%) and subsequently with acetic acid-EtOH. Thanks, Gil >-- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 _______________________________________________ From ROrr <@t> northshore.org Thu Aug 5 17:33:16 2010 From: ROrr <@t> northshore.org (Orr, Rebecca) Date: Thu Aug 5 17:33:20 2010 Subject: [Histonet] Pen Slides for LCD In-Reply-To: <852b90e1-7a29-4d99-ba4f-149c48f686dd@EXCHCAS01.enhnet.org> Message-ID: Hi Mary, We had a similar issue. I was asked to troubleshoot this problem for our researchers...and I didn't make a copy of the literature and gave it away....or I'd quote directly... You'll have to call Leica and get the specific tech support area that handles LCD. They have a "memo" that she sent me that describes curing the membrane slides under UV for a specific amount of time (I think 1/2 hour) before picking up tissue. The product insert that comes with the slides do not address this. Once we did the UV step it was fine, tissue did not wash off. She was very nice and very knowledgeable and sent me the memo no probs. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 Message: 14 Date: Thu, 5 Aug 2010 12:51:48 -0400 From: "Mary Lloyd" Subject: [Histonet] PEN SLIDES FOR LASER CAPTURE To: Message-ID: <04A71EFE05FE5F4DAA05D0500FD0597002A0161E@Distal.dentnet.dent.ohio-state.edu> Content-Type: text/plain; charset="us-ascii" Does anyone have any experience with PEN membrane slides from Zeiss for laser capture microdissection. I have been working with one of our residents and we are having problems with the tissue staying on these slides. We could sure use help. Thanks Mary Lloyd From laurie <@t> conxis.com Thu Aug 5 18:49:28 2010 From: laurie <@t> conxis.com (Laurie Popp) Date: Thu Aug 5 18:50:13 2010 Subject: [Histonet] Collagen 1 staining in FFPE tissue Message-ID: <4C5B4E08.1040401@conxis.com> Hello everyone, I was wondering if anyone has a good protocol for staining collagen 1 in FFPE tissue? I am looking for something that will work in rabbit or rat. Thanks, Laurie From jhabecke <@t> fhcrc.org Thu Aug 5 18:59:44 2010 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Thu Aug 5 18:59:49 2010 Subject: [Histonet] Turnaround time for Research Pathology Message-ID: <040346FA7309BD439C327F97D4C4D69B09DE2206@ISIS.fhcrc.org> Folks, I am looking for some standard turnaround times for histology services including processing to embedding, processing to H&E, and processing to H&E and IHC. Does anyone have that data? Also, how do other folks handle "standard turnaround time" for research samples? We may get anything from 5 specimens to 300 specimens that just need paraffin processing and 1 H&E. Or we may get a request for 10 samples for paraffin-processing, 10 slides each with 2 H&E and 7 different IHC stains per case. Any insight on this would be very helpful. Thanks, Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabecke@fhcrc.org From jaylundgren <@t> gmail.com Thu Aug 5 21:51:30 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Aug 5 21:51:35 2010 Subject: [Histonet] Turnaround time for Research Pathology In-Reply-To: <040346FA7309BD439C327F97D4C4D69B09DE2206@ISIS.fhcrc.org> References: <040346FA7309BD439C327F97D4C4D69B09DE2206@ISIS.fhcrc.org> Message-ID: Julie, I've worked in both the clinical and research environments, and the turnaround time depends on which you're talking about. 200-400 blocks a day with about 50% multiple levels and unstained slides, 30-70 immunos, and one or two dozen special stains is a normal day at the hospital lab I'm currently working at. That's a less than 24 hour turn around time for four busy histotechs. On the other hand, for most research labs I've worked at, the turnaround time would be.... whenever. Sounds to me like your PI's are wanting their stuff out faster. Maybe you are understaffed. Don't expect much sympathy from clinical lab folks. Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonya.martin <@t> soton.ac.uk Fri Aug 6 03:12:53 2010 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Fri Aug 6 03:13:40 2010 Subject: [Histonet] Spleen EM images Message-ID: <5F338719C0D0DE44BDFDD2B83D3FF7A1CADEF5BE5D@UOS-CL-EX7-L3.soton.ac.uk> Hi there, Just wondering if there are any EM experts out there who could look at some EM of mouse spleen sections for me and annotate them. I do a lot of LM work on spleen sections and I had the chance to try some EM but unfortunately nobody could help me identify what sort of cells I was looking at. I've tried comparing them to some published studies but cant be sure if I'm right about anything! Anyway if anyone has a bit of spare time....... I can email you the images, Thanks Sonya ------------------------------------------------------------ Dr Sonya James Microscopy Support Officer University of Southampton UK Email: sm2300@soton.ac.uk From Melissa.Kuhnla <@t> chsli.org Fri Aug 6 05:02:47 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Fri Aug 6 05:04:44 2010 Subject: [Histonet] Ventana IHC/ recycled reagents Message-ID: Anyone running IHC on Ventana platforms? Do you run patient samples on the same slide as control tissue? Do you have problems with sections adhering to the slides? The largest culprit for us seems to be small/fine needle core biopsies. Any insight into why this shape is problematic? Over-processed maybe? Is anyone using recycled alcohols or Xylene in their tissue processors? Do you find this to be problematic? Thank you, Melissa Lead Med. Technologist, IHC/ISH Catholic Health Services of Long Island The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From BSullivan <@t> shorememorial.org Fri Aug 6 06:09:42 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Fri Aug 6 06:12:18 2010 Subject: [Histonet] Ventana IHC/ recycled reagents In-Reply-To: Message-ID: We try to run both the positive control and patient tissue on the same slide when size allows this. There are times, especially with cell blocks that we have problems with tissue loss. We have started to mount all cell blocks on their own slide. One thing that we have implemented and seems to work great is making sure that all debris, and this includes melted paraffin from the control, be removed from the control slide. A clean slide is important for adherence of the tissue. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Kuhnla, Melissa" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Ventana IHC/ recycled reagents 08/06/2010 06:02 AM Anyone running IHC on Ventana platforms? Do you run patient samples on the same slide as control tissue? Do you have problems with sections adhering to the slides? The largest culprit for us seems to be small/fine needle core biopsies. Any insight into why this shape is problematic? Over-processed maybe? Is anyone using recycled alcohols or Xylene in their tissue processors? Do you find this to be problematic? Thank you, Melissa Lead Med. Technologist, IHC/ISH Catholic Health Services of Long Island The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Aug 6 07:47:52 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Aug 6 07:47:56 2010 Subject: [Histonet] Ventana IHC/ recycled reagents In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E2738399F39@UWHC-MAIL01.uwhis.hosp.wisc.edu> Melissa, First, I would make sure that when you create your control slides that you only dip the top end of the slide into your water bath to pick up your control tissue section thus the bottom part of the slide where the patient tissue will go has never been wet or collected debris from your water bath. Second, I would pick up your sections with core/needle biopsies so that the tissue runs parallel to the long edges of the slide. Your wash buffers will encounter less resistance from the tissue this way and hopefully not so readily dislodge your tissue. Just a couple thoughts that might help. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Friday, August 06, 2010 5:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana IHC/ recycled reagents Anyone running IHC on Ventana platforms? Do you run patient samples on the same slide as control tissue? Do you have problems with sections adhering to the slides? The largest culprit for us seems to be small/fine needle core biopsies. Any insight into why this shape is problematic? Over-processed maybe? Is anyone using recycled alcohols or Xylene in their tissue processors? Do you find this to be problematic? Thank you, Melissa Lead Med. Technologist, IHC/ISH Catholic Health Services of Long Island The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Fri Aug 6 10:14:55 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri Aug 6 10:15:03 2010 Subject: [Histonet] Re: Turnaround time for Research Pathology Message-ID: Julie, You asked about standard turnaround times for histology services in the Research environment. As you eluded to in your second paragraph, rarely do we have standard projects. Often we're asked to do serial sectioning of an entire organism and we have no idea how many slides we'll end up with, or how long it'll take is to do each one. Someone can submit one sample, or 30 samples. Paraffin processing and 1 H&E can be turned over fairly quickly depending on the existing work queue. With some exceptions we take things as first in, first out. Here's what I have on my intranet page: Turn-around Time: It is our goal to supply completed work to the researcher within 3 to 7 working days from submission. Fixation, decalcification, special handling, or other procedural steps may delay completion. Any special turn-around needs should be communicated to the histology staff and we will do our best to meet them. There are times we can receive a sample, process it over night, section and stain it the next day and have it completed in 24 hours. The researchers are always delighted and surprised when we can do this. It's more usual that we can turn around fairly routine and fairly simple requests within 3 working days. If we have a long work queue, we try to let the researcher know there might be a delay in getting their samples back as soon as they submit the sample regardless of what the services are. We always will move things up the queue when it's needed for publication submission reviews or other special circumstances. Communication is critical. Give them a reasonable timeline (you may have to determine this yourself based on usual workload and staffing). Give them timely information when there are circumstances which interfere with achieving that timeline, and update it when you need to. Finally, work together when exceptions need to be made. It's worked out well for us so far. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From Montina.VanMeter <@t> pbrc.edu Fri Aug 6 11:36:32 2010 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Fri Aug 6 11:43:14 2010 Subject: [Histonet] RE: floating section incubation times In-Reply-To: <20100805154530.CHY83424@m4500-03.uchicago.edu> References: <20100805154530.CHY83424@m4500-03.uchicago.edu> Message-ID: <4FE7FB862E90E448AE32388E759220E5789D78@pbrcas31.pbrc.edu> David, The reason that I am able incubate overnight is that I antigen retrieve my free-floating sections in the Biocare Decloaker (and no, I am not receiving any subsidies from them). This has enabled me to reduce incubation times as well as revealing receptor staining that has been difficult to observe in the past due to the lack of sensitive techniques. I am serum-free and use polymers for detection. This has drastically cut down on my background issues. Regards, Tina -----Original Message----- From: David A. Wright [mailto:dw18@uchicago.edu] Sent: Thursday, August 05, 2010 3:46 PM To: histonet@lists.utsouthwestern.edu Cc: Montina Van Meter; Guillermo Palchik Subject: Re: floating section incubation times Hi Histonet, Tina & Gil I work on the same material as Tina and just wanted to echo her "overnight" comments with the addition that I routinely incubate 40um floating sections over the whole weekend at room temp (on a very slow shaker, 20 rpm, and with azide to stop bug growth. It all washes out before the peroxidase reaction.) I seem to remember from Fick's Law that the time taken to diffuse to the same endpoint concentration goes up with the square of the thickness, so if you double the thickness you would need 4x the incubation time - or else more concentrated antibody. The same distinction applies to "floating" vs on-slide incubations. {It wasn't clear to me if Gil's sections were floating or not). Since reagents can penetrate from both sides of a floating section, a section stained "on-slide" is effectively twice as thick and needs 4x the time. Note that the same principle applies to leaching out unbound reagents - you should lengthen your wash steps too, by the same factor, to prevent high background. happy staining -David David A. Wright, PhD University of Chicago Section of Neurosurgery ---- Original message ---- Date: Thu, 05 Aug 2010 12:07:03 -0500 (CDT) Subject: Histonet Digest, Vol 81, Issue 5 Message: 1 Date: Wed, 4 Aug 2010 12:18:03 -0500 From: "Montina Van Meter" Subject: RE: [Histonet] TUNEL of frozen thick rat brain slices. Gil, I routinely incubate free-floating 40um rat brain sections overnight or longer @ 4 degrees centigrade according to the particular antibody that I am working with. Our sections are perfused in 4% paraformaldehyde and cryoprotected with 30% sucrose prior to sectioning. How long are you fixing the tissue prior to staining? Tina >-----Original Message----- On Behalf Of Guillermo Palchik Sent: Wednesday, August 04, 2010 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TUNEL of frozen thick rat brain slices. Dear Histologists, Has anyone done TUNEL on 40 um flash-frozen rat brain sections? I have tried tweaking the protocol (we use the Millipore Apoptag kit - S7101)from our ongoing 20 um slices by increasing the incubation times from 1 hour to 2 and 4 hours but we still get poor staining. Also, could I do overnight incubations? the tissue is flash frozen, but it does get fixed at the beginning of the protocol with PFA (4%) and subsequently with acetic acid-EtOH. Thanks, Gil >-- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 _______________________________________________ From Sharon.Davis-Devine <@t> carle.com Fri Aug 6 11:45:34 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Fri Aug 6 11:45:39 2010 Subject: [Histonet] Her2 question for tissue processors Message-ID: Are there any validation requirements for tissue processing Her2 neu specimens? Thanks for the info. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From AHutton <@t> dh.org Fri Aug 6 12:26:52 2010 From: AHutton <@t> dh.org (Hutton, Allison) Date: Fri Aug 6 12:28:47 2010 Subject: [Histonet] PA coverage Message-ID: <38A56C4F4630D348A50B3720409270870E0FE1D1@dhmail.dhorg.org> We are currently looking for a pathologist assistant to cover some upcoming vacations. Must be able to cut frozen sections. We are a community hospital located in southeastern PA. Please foward any questions or inquiries to the email / number below. Thank you Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital 595 W. State St Doylestown, PA 18901 215-345-2264 ahutton@dh.org From Marilyn.A.Weiss <@t> kp.org Fri Aug 6 12:36:29 2010 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Aug 6 12:36:36 2010 Subject: [Histonet] I will be out of office beginning the afternoon of 8/6 2010 and returning 8/13/2010 Message-ID: I will be out of the office starting 08/06/2010 and will not return until 08/13/2010. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. From baderbo <@t> gmail.com Fri Aug 6 12:51:05 2010 From: baderbo <@t> gmail.com (Bader Siddiki) Date: Fri Aug 6 12:51:08 2010 Subject: [Histonet] Rcd e-mail on Histonet regarding a good place to get eletronics etc. Message-ID: Hello Histonetters I hope you guys and gals are getting ready for the weekend, have a good one. Few months ago we received an e-mail on histonet that there was a place on internet one can purchase electronics at reasonable prices. Did any order anything from them, are they for real. I lost that e-mail and web site address, if you have this info. We Bader Have a nice day/weekend Mit freundlichen Gr??en / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten ?????? Bader From bbroders <@t> unlnotes.unl.edu Fri Aug 6 13:25:56 2010 From: bbroders <@t> unlnotes.unl.edu (Bruce W Brodersen) Date: Fri Aug 6 13:26:14 2010 Subject: [Histonet] friable or crumbly O.C.T. Message-ID: Anyone have an explanation as to why OCT would be friable or crumbly for sectioning? Here's how it was used. Thanks. "We held the plastic 'tray' with the tissue in the compound just over the liquid nitro for 30sec-1min, until it was opaque and white (frozen) and then dipped the tray into the liquid nitro for 20-30sec., placed in small bags and then into a cooler with dry ice until shipping." Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094 From TGoins <@t> mt.gov Fri Aug 6 14:13:17 2010 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Aug 6 14:13:24 2010 Subject: [Histonet] de-wax without organics Message-ID: We have run some preliminary tests on de-waxing slides using warm detergent solutions rather than xylene or xylene substitutes. So far the pathologists see no difference between the H&E stained slides treated with detergent vs. the usual xylene to alcohol to water route; the cellular detail is equivalent and the dye reactions appear identical. We have also run comparisons for several special stains and IHC slides with good results. I would greatly appreciate hearing from anyone who is aware of reasons why this is a bad idea! It is quicker and reduces the volume of organics required by half so we are seriously considering it as an alternative protocol. Thanks in advance for your feedback. Tresa Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana From LSebree <@t> uwhealth.org Fri Aug 6 14:37:34 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Aug 6 14:37:39 2010 Subject: [Histonet] de-wax without organics In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E2738399F3D@UWHC-MAIL01.uwhis.hosp.wisc.edu> Tresa, This is exactly how our Ventana instruments deparaffinize our slides and we've never had a problem regarding this methodology. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Friday, August 06, 2010 2:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] de-wax without organics We have run some preliminary tests on de-waxing slides using warm detergent solutions rather than xylene or xylene substitutes. So far the pathologists see no difference between the H&E stained slides treated with detergent vs. the usual xylene to alcohol to water route; the cellular detail is equivalent and the dye reactions appear identical. We have also run comparisons for several special stains and IHC slides with good results. I would greatly appreciate hearing from anyone who is aware of reasons why this is a bad idea! It is quicker and reduces the volume of organics required by half so we are seriously considering it as an alternative protocol. Thanks in advance for your feedback. Tresa Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From k84as <@t> yahoo.com Fri Aug 6 14:56:47 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Aug 6 14:56:51 2010 Subject: [Histonet] clean slides Message-ID: <86318.30315.qm@web112612.mail.gq1.yahoo.com> Dear Histnetters i have a proplem that when i open a new slide pox i see some depress on slides such as dust so i'm trying to clean it by a formula i have read about it by using potassium dichromat and sulphoric acid for cleaning glassware. i just would like you to share me for that issue what are you doing? ? thanx Moahmed Abd El Razik Dep. Of Histology Faculty Of Vet. Med. Cairo Univ. in Egypt From JWeems <@t> sjha.org Fri Aug 6 15:10:11 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Aug 6 15:10:14 2010 Subject: [Histonet] clean slides In-Reply-To: <86318.30315.qm@web112612.mail.gq1.yahoo.com> References: <86318.30315.qm@web112612.mail.gq1.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640332099472@CHEXCMS10.one.ads.che.org> Bless your heart!! That is strong stuff. I would try dipping or wiping each slide with a gauze in absolute alcohol - and stand to let dry. Or put the slides in a rack and dip up and down to agitate in the alcohol. Would that work? Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, August 06, 2010 15:57 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] clean slides Dear Histnetters i have a proplem that when i open a new slide pox i see some depress on slides such as dust so i'm trying to clean it by a formula i have read about it by using potassium dichromat and sulphoric acid for cleaning glassware. i just would like you to share me for that issue what are you doing? ? thanx Moahmed Abd El Razik Dep. Of Histology Faculty Of Vet. Med. Cairo Univ. in Egypt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rjbuesa <@t> yahoo.com Fri Aug 6 15:13:41 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 6 15:13:45 2010 Subject: [Histonet] de-wax without organics In-Reply-To: Message-ID: <942920.45179.qm@web65712.mail.ac4.yahoo.com> Under separate cover I am sending an article on the subject of dewaxing with dishwaser liquid soap. Ren? J. --- On Fri, 8/6/10, Goins, Tresa wrote: From: Goins, Tresa Subject: [Histonet] de-wax without organics To: "Histonet@lists.utsouthwestern.edu" Date: Friday, August 6, 2010, 3:13 PM We have run some preliminary tests on de-waxing slides using warm detergent solutions rather than xylene or xylene substitutes.? So far the pathologists see no difference between the H&E stained slides treated with detergent vs. the usual xylene to alcohol to water route; the cellular detail is equivalent and the dye reactions appear identical.???We have also run comparisons for several special stains and IHC slides with good results. I would greatly appreciate hearing from anyone who is aware of reasons why this is a bad idea!? It is quicker and reduces the volume of organics required by half so we are seriously considering it as an alternative protocol. Thanks in advance for your feedback. Tresa Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Fri Aug 6 15:16:38 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Fri Aug 6 15:16:54 2010 Subject: [Histonet] clean slides In-Reply-To: <86318.30315.qm@web112612.mail.gq1.yahoo.com> References: <86318.30315.qm@web112612.mail.gq1.yahoo.com> Message-ID: <4C5C6DA6.8070606@vneubert.com> Ordinary slides? Wiping on the sleeve of my lab coat was okay :) > Dear Histnetters > i have a proplem that when i open a new slide pox i see some depress on slides such as dust > so i'm trying to clean it by a formula i have read about it by using potassium dichromat and sulphoric acid for cleaning glassware. > i just would like you to share me for that issue > what are you doing? > > thanx > Moahmed Abd El Razik > Dep. Of Histology > Faculty Of Vet. Med. > Cairo Univ. in Egypt > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Fri Aug 6 19:41:53 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Aug 6 19:42:00 2010 Subject: [Histonet] PEN SLIDES FOR LASER CAPTURE Message-ID: Hi, I do sectioning on LCM slides frequently. The problem of tissue coming off the slides si kinda the whole point of the LCM slides. The tissue is supposed to come off, otherwise it would be impossible to capture the tissue with the laser. This of course poses a problem for staining. First you need to figure out what you are extracting. If it is for PCR for DNA you are in luck, RNA causes more complications because of the RNAases that can destroy the yield. You will not be able to stain the tissue the same way you would for regular charged or adhesive slides. You can heat the slides some, but bear in mind this will increase RNAase activity. Xylene will cause the plastic membrane to shrivel and fall from the slide, so you will need to abbreviate the deparaffinization to only a couple of minutes. Yes this will probably cause the staining to be less than perfect, but perfect staining is not really needed on LCM slides (If you want perfection use charged slides). Rehydrate quickly and gently, shorten the time in the stains as best as you can. Best of Luck, Amos Message: 14 Date: Thu, 5 Aug 2010 12:51:48 -0400 From: "Mary Lloyd" Subject: [Histonet] PEN SLIDES FOR LASER CAPTURE To: Message-ID: < 04A71EFE05FE5F4DAA05D0500FD0597002A0161E@Distal.dentnet.dent.ohio-state.edu> Content-Type: text/plain; charset="us-ascii" Does anyone have any experience with PEN membrane slides from Zeiss for laser capture microdissection. I have been working with one of our residents and we are having problems with the tissue staying on these slides. We could sure use help. Thanks Mary Lloyd From amosbrooks <@t> gmail.com Fri Aug 6 20:23:50 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Aug 6 20:23:54 2010 Subject: [Histonet] Staining Racks Message-ID: Hi, I recently acquired a used coverslipper (YEAY ME!!!) It is a Leica CV-5000. I know there are a number of big fans of this coverslipper here, but I have a possible problem. I also have an automatic slide stainer that I LOVE! The stainer is a Sakura DRS-2000. It has a slide rack holder that holds 2 Winlab 20 slide racks (the dusty charcoal colored racks). The Leica takes a rack that holds 30 or so slides with a hanger on either side. This of course doesn't fit the stainer or the stainer rack holder. So here's the conondrum, how do I get these instruments to play nicely together? Is there a rack holder that I could get for the Sakura stainer that would hold the Leica racks? Alternatively, is there any way to use the Winlab racks for the Sakura on the Leica coverslipper without having the slides jam? (coverslippers are ALL finicky) Are there any alternative solutions anyone has found? I had considered fabricating a stainer rack holder that would hold the Leica racks with plexiglass perhaps, but if there is a solution that is already being used I'd love to save the experimentation. I certainly won't transfer the slides between racks after staining. I might as well hand coverslip in that case. Thanks in advance for any help! Happy Friday, Amos From gu.lang <@t> gmx.at Sat Aug 7 05:02:41 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Aug 7 05:02:48 2010 Subject: [Histonet] LCM question Message-ID: <10B2D9487EDC4771BC617B89964586AF@dielangs.at> Hi, I'm interested in LCM-Systems. I'd like to know, if there are systems, that can transfer cut regions of the donor-section on to other slides for further staining like FISH. Until now I've found only internet-information about systems for harvesting tissue or cells for further molecular testing, where the tissue is desintegrated after harvesting. Perhaps someone can provide some information like an weblink. Thanks Gudrun From gu.lang <@t> gmx.at Sat Aug 7 05:12:08 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Aug 7 05:12:16 2010 Subject: [Histonet] how to make crashed ice? Message-ID: Hi! I think this is a rather basic question ;-), but I'm looking for practical advice. We are going to try the OSNA-test for sentinel nodes. The application needs a pot with crashed ice while desintegrating the tissue with a mixer. So over the day this should be four to six litre ice, if we have to take fresh ice for each time, a group of sentinels has to be worked up. What is a practical way to make crashed ice in the lab? Thanks for your answers in advance! Gudrun From louise.renton <@t> gmail.com Sat Aug 7 05:24:17 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Sat Aug 7 05:24:21 2010 Subject: [Histonet] how to make crashed ice? In-Reply-To: References: Message-ID: Hi gudrun, Place the ice - ice cubes works best in a plastic bag, wrap in a towel and bash with a heavy object like a hammer. You can also use Jamie Oliver's trick - put ice cubes in a cloth tea towel, , bring the 4 corners together, tie them in knot, and then hit the "parcel" on the edge of your work surface until the ice is the sze you need.... hope this helps On Sat, Aug 7, 2010 at 12:12 PM, Gudrun Lang wrote: > Hi! > > I think this is a rather basic question ;-), but I'm looking for practical > advice. > > We are going to try the OSNA-test for sentinel nodes. The application needs > a pot with crashed ice while desintegrating the tissue with a mixer. So > over > the day this should be four to six litre ice, if we have to take fresh ice > for each time, a group of sentinels has to be worked up. > > > > What is a practical way to make crashed ice in the lab? > > Thanks for your answers in advance! > > Gudrun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From koellingr <@t> comcast.net Sat Aug 7 12:12:45 2010 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Aug 7 12:12:49 2010 Subject: [Histonet] how to make crashed ice? In-Reply-To: Message-ID: <224222764.880495.1281201165276.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Hello, It is very inexpensive, a few dollars, to buy a counter-top?ice crusher, that a professional bartender might use to crush ice for drinks.? Can be bought at most any store.? We?crush ice?every day on a daily basis to create a small tub of? ice we need for reagents.? Only the size of like a small kitchen food processor. Just add ice cubes and crush them in a few seconds without a hammer and the mess. Ray PhenoPath Labs Seattle, WA ----- Original Message ----- From: "louise renton" To: "gu lang" , Histonet@lists.utsouthwestern.edu Sent: Saturday, August 7, 2010 3:24:17 AM Subject: Re: [Histonet] how to make crashed ice? Hi gudrun, Place the ice - ice cubes works best in a plastic bag, wrap in a towel and bash with a heavy object like a hammer. You can also use Jamie Oliver's trick - put ice cubes in a cloth tea towel, , bring the ?4 corners together, tie them in knot, and then hit the "parcel" on the edge of your work surface until the ice is the sze you need.... hope this helps On Sat, Aug 7, 2010 at 12:12 PM, Gudrun Lang wrote: > Hi! > > I think this is a rather basic question ;-), but I'm looking for practical > advice. > > We are going to try the OSNA-test for sentinel nodes. The application needs > a pot with crashed ice while desintegrating the tissue with a mixer. So > over > the day this should be four to six litre ice, if we have to take fresh ice > for each time, a group of sentinels has to be worked up. > > > > What is a practical way to make crashed ice in the lab? > > Thanks for your answers in advance! > > Gudrun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sat Aug 7 12:30:58 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Aug 7 12:31:03 2010 Subject: [Histonet] de-wax without organics Message-ID: Hi, I am curious what temperature and percent detergent you are using. Also which detergent did you use? Some are different than others. Do you have a list of special stains you have tried? I am sure this will probably work great, I just think that it is conceivable for different labs using different detergents, different concentrations and different temperatures to get different results with some stains. This is a really cool subject and if you could compile the information into an article I could find it a home in the Paraffin Press for the Connecticut Society. Just in case you are interested. Thanks, Amos Message: 5 Date: Fri, 6 Aug 2010 19:13:17 +0000 From: "Goins, Tresa" Subject: [Histonet] de-wax without organics To: "Histonet@lists.utsouthwestern.edu" Message-ID: < CA4DF32ED505D94BB55E95487D8E984104FD48@DOAISD5205.state.mt.ads> Content-Type: text/plain; charset="us-ascii" We have run some preliminary tests on de-waxing slides using warm detergent solutions rather than xylene or xylene substitutes. So far the pathologists see no difference between the H&E stained slides treated with detergent vs. the usual xylene to alcohol to water route; the cellular detail is equivalent and the dye reactions appear identical. We have also run comparisons for several special stains and IHC slides with good results. I would greatly appreciate hearing from anyone who is aware of reasons why this is a bad idea! It is quicker and reduces the volume of organics required by half so we are seriously considering it as an alternative protocol. Thanks in advance for your feedback. Tresa Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana From napoli <@t> siscom.net Sun Aug 8 12:10:54 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Sun Aug 8 12:10:57 2010 Subject: [Histonet] CA4DF32ED505D94BB55E95487D8E984104FD48@DOAISD5205.state.mt.ads Message-ID: <4c5ee51e.73.527e.1030580047@siscom.net> I am wondering how much agitation is required in order to achieve the clearing of paraffin from the tissue and slide. IHC systems obviously use adhesive or + slides, aiding in tissue adherence. Too much agitation might take take tissue off. And what about nail fragments or hard tissues in general? Will they survive? I have an article and info regarding this type of deparaffinization and will try to get it into an e-mail. My sense is that it could very well be less efficient and time consuming and that depending on how it is done, could yield very different results. I still think that "there's nothing like xylene!!!" But obviously soap and water doesnt hurt your liver.... Interesting method I would like to hear more about people's experiences. From Steven.Weston <@t> utas.edu.au Sun Aug 8 23:32:52 2010 From: Steven.Weston <@t> utas.edu.au (Steven Weston) Date: Sun Aug 8 23:33:01 2010 Subject: [Histonet] Re tunel staining Message-ID: DQpJIHJlY2VudGx5IGhhZCBhbiBlbnF1aXJ5IGZyb20gb25lIG9mIG91ciBwb3N0IGRvY3Mgd2hv IHdhcyBsb29raW5nIGF0IFRVTkVMIHN0YWluaW5nIGFuZCBjYW1lIGFjcm9zcyB0aGlzIHJlZmVy ZW5jZSBpbiBiaW9jaGVtaWNhIG5vIDQgKDE5OTcpdGl0bGVkDQqhp0ZpeGF0aW9uIG9mIFRpc3N1 ZSBTZWN0aW9ucyBmb3INClRVTkVMIENvbWJpbmVkIHdpdGggU3RhaW5pbmcgZm9yDQpUaHltaWMg RXBpdGhlbGlhbCBDZWxsIE1hcmtlcqGoDQpZb3Ugc2hvdWxkIGJlIGFibGUgdG8gZ29vZ2xlIGl0 IGFuZCBmaW5kIHRoZSBhcnRpY2xlLg0KQmFzaWNhbGx5IGl0IHNhaWQgdGhhdCBhZnRlciBmaXhh dGlvbiB3aXRoIFBGQSB0byBpbXByb3ZlIHN0YWluaW5nIHlvdSBuZWVkIHRvIHRyZWF0IHdpdGgg dHJpdG9uIGFuZCBzb2RpdW0gY2l0cmF0ZSBpbiB0aGUgY29sZCB0byBleHBvc2UgdGhlIGFudGln ZW4gYWdhaW4uDQpCZWxvdyBpcyB0aGUgc3VnZ2VzdGVkIHByb3RvY29sIGZvciB0aGluIGNyeW9z dGF0IHNlY3Rpb25zLiBJoaZtIHN1cmUgaXQgY291bGQgYmUgbW9kaWZpZWQgZm9yIHRoaWNrIHNl Y3Rpb25zLg0KDQoNCjEuIEN1dCA0IKNnbSB0aGluIGNyeW9zZWN0aW9ucyBhbmQgbW91bnQgb24N CnBvbHlseXNpbmUtY29hdGVkIGdsYXNzIHNsaWRlcy4NCjIuIEFpciBkcnkgb3Zlcm5pZ2h0IGF0 IHJvb20gdGVtcGVyYXR1cmUgYW5kDQpmcmVlemUgZm9pbC0gd3JhcHBlZCBzbGlkZXMgYXQgoVYy MKJYQyB1bnRpbCB1c2UuDQozLiBBcHBseSBmcm96ZW4gZ2xhc3Mgc2xpZGVzIGRpcmVjdGx5IGlu dG8gYSBjb250YWluZXINCndpdGggMSUgYnVmZmVyZWQgcGFyYWZvcm1hbGRlaHlkZSBmb3INCjMw IG1pbiBhdCBSVC4NCjQuIFJpbnNlIHN3aWZ0bHkgaW4gUEJTIGFuZCBpbW1lcnNlIGluIGEgc29s dXRpb24NCm9mIDElIFRyaXRvbiBYMTAwICh2L3YpIGFuZCAxJSBzb2RpdW0NCmNpdHJhdGUgKHcv dikgZm9yIDIgbWluIGF0IDSiWEMuDQo1LiBXYXNoIGluIFBCUyBhbmQgaW5jdWJhdGUgd2l0aCA1 MCCjZ2wgVFVORUwNCnJlYWN0aW9uIG1peHR1cmUgKFRkVCBzb2x1dGlvbiB3aXRoIGRVVFAtRklU Qw0Kc29sdXRpb24sIDErOSkuIENvbnZlcnQgdG8gZW56eW1lIGxhYmVsIGlmDQpkZXNpcmVkLg0K Ni4gV2FzaCB3aXRoIFBCUyBhbmQgYmxvY2sgd2l0aCBkaWx1dGlvbiBvZg0KYWRlcXVhdGUgbm9y bWFsIHNlcnVtLg0KMTAuIFByb2NlZWQgd2l0aCBkZXNpcmVkIGltbXVub2hpc3RvY2hlbWljYWwN CmxhYmVsaW5nLg0KDQpSZWdhcmRzDQpTdGV2ZSBXZXN0b24NClNlbmlvciB0ZWNobmljYWwgb2Zm aWNlcg0KTWVuemllcyBSZXNlYXJjaCBJbnN0aXR1dGUNCkhvYmFydCBUYXNtYW5pYQ0K From MSHERWOOD <@t> PARTNERS.ORG Mon Aug 9 09:32:48 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Aug 9 09:32:53 2010 Subject: [Histonet] Re: [Special Stains] Masson's Trichrome Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24532@PHSXMB30.partners.org> To all: We keep having the same discussion re: special stains. How long can you use them before discarding? I am specifically referring to the stains used in Masson's Trichrome. We are a research lab and, therefore, don't run the volume that most labs do. We have found, for instance, that we can use the Weigert's Hematoxylin (A&B) more than once, with filtering (even though it is stated it should be made fresh). We also use Bouin's Fix, Biebrich Scarlet and Aniline Blue more than once. Is there a "set rule" when we should discard these solutions (i.e. after 5X use or 1 month)? I would appreciate some feedback from the experts! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From dellav <@t> musc.edu Mon Aug 9 09:40:03 2010 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Mon Aug 9 09:40:34 2010 Subject: [Histonet] friable or crumbly O.C.T. In-Reply-To: References: Message-ID: I'm guessing that liquid nitrogen or dry ice temperature is too cold for sectioning OCT. OCT cuts well down to about -25 degrees C. Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the same temp range at LN2 You will want to give the OCT blocks the opportunity to "warm up" to cryostat temperature before attempting to section them. Leave your frozen blocks in the cryostat for 30-60 minutes before sectioning to allow them to come to optimum temperature Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce W Brodersen Sent: Friday, August 06, 2010 2:26 PM To: histonet@pathology.swmed.edu Subject: [Histonet] friable or crumbly O.C.T. Anyone have an explanation as to why OCT would be friable or crumbly for sectioning? Here's how it was used. Thanks. "We held the plastic 'tray' with the tissue in the compound just over the liquid nitro for 30sec-1min, until it was opaque and white (frozen) and then dipped the tray into the liquid nitro for 20-30sec., placed in small bags and then into a cooler with dry ice until shipping." Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jsjurczak <@t> comcast.net Mon Aug 9 09:49:11 2010 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Mon Aug 9 09:49:17 2010 Subject: [Histonet] friable or crumbly O.C.T. In-Reply-To: Message-ID: <2100844269.41947.1281365351046.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> There is an OCT for lower temperatures. ----- Original Message ----- From: "Della Speranza, Vinnie" To: "Bruce W Brodersen" , histonet@pathology.swmed.edu Sent: Monday, August 9, 2010 9:40:03 AM Subject: RE: [Histonet] friable or crumbly O.C.T. I'm guessing that liquid nitrogen or dry ice temperature is too cold for sectioning OCT. OCT cuts well down to about -25 degrees C. Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the same temp range at LN2 You will want to give the OCT blocks the opportunity to "warm up" to cryostat temperature before attempting to section them. Leave your frozen blocks in the cryostat for 30-60 minutes before sectioning to allow them to come to optimum temperature Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue ?Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce W Brodersen Sent: Friday, August 06, 2010 2:26 PM To: histonet@pathology.swmed.edu Subject: [Histonet] friable or crumbly O.C.T. Anyone have an explanation as to why OCT would be friable or crumbly for sectioning? ?Here's how it was used. Thanks. "We held the plastic 'tray' with the tissue in the compound just over the liquid nitro for 30sec-1min, until it was opaque and white (frozen) and then dipped the tray into the liquid nitro for 20-30sec., placed in small bags and then into a cooler with dry ice until shipping." Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE ?68583-0907 voice (402) 472-1434 FAX (402 472-3094_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Mon Aug 9 09:53:48 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Aug 9 09:53:52 2010 Subject: [Histonet] Anti-IDO (indolamine 2,3-dioxygenase) Message-ID: Hi Histonet, Does anyone use an Anti-IDO on FFPE tissues? Would you please let me know where you purchase it? Thank you, Mark Tarango From renafail <@t> bellsouth.net Mon Aug 9 10:24:01 2010 From: renafail <@t> bellsouth.net (Rena Fail) Date: Mon Aug 9 10:24:07 2010 Subject: [Histonet] Re: [Special Stains] Masson's Trichrome In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24532@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24532@PHSXMB30.partners.org> Message-ID: <426670.14254.qm@web180808.mail.gq1.yahoo.com> It depends on the number of slides as well as time on the shelf and a little common sense.Your stock solutions have a longer shelf life than?your working. ?I worked in a lab that perfomed a large number of Masson's by hand( until purchasing the Artisan)? We changed the solutions once a week to insure consistent staining.? If you perform these stains only twice a month, your results will be more consistant if you make the Weigert's fresh? and watch the level of your aniline blue, evaporation will make it more concentrated.? On the other hand a large volume of slides will weaken the?working solutions. following is a link that lists some guidelines for storing stock solutions Rena Fail www.urmc.rochester.edu/.../StainsManual/STABILITYOFSPECIALSTAININGSOLUTIONS.html?...- Cached ----- Original Message ---- From: "Sherwood, Margaret" To: histonet@lists.utsouthwestern.edu Sent: Mon, August 9, 2010 10:32:48 AM Subject: [Histonet] Re: [Special Stains] Masson's Trichrome To all: We keep having the same discussion re:? special stains.? How long can you use them before discarding?? I am specifically referring to the stains used in Masson's Trichrome. We are a research lab and, therefore, don't run the volume that most labs do. We have found, for instance, that we can use the Weigert's Hematoxylin (A&B) more than once, with filtering (even though it is stated it should be made fresh).? We also use Bouin's Fix, Biebrich Scarlet and Aniline Blue more than once.? Is there a "set rule" when we should discard these solutions (i.e. after 5X use or 1 month)? I would appreciate some feedback from the experts! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Aug 9 10:50:21 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Aug 9 10:50:24 2010 Subject: [Histonet] RELIA Histology Job Alert 8-09-10 Histotech needed in Charlotte, NC Message-ID: Hi Histonetters!! I hope everyone had a great weekend. I have an exciting new opportunity to tell you about. I am working with a premier client located Charlotte, NC. This is a part time (32 hours per week) permanent position in a full service histology lab. My client is looking for an ASCP certified histotech with at least 2 years of histology experience must be able to meet CLIA requirements to do grossing. (My client does mainly simple grossing, mainly biopsies and excisions). My client offers excellent pay and benefits. If you or anyone you know would like more information please contact me at 866-607-3542 or relia1@earthlink.net Have a Great Day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From bill501 <@t> mindspring.com Mon Aug 9 10:55:40 2010 From: bill501 <@t> mindspring.com (Bill B.) Date: Mon Aug 9 10:55:52 2010 Subject: [Histonet] friable or crumbly O.C.T. In-Reply-To: References: Message-ID: I will 2nd this. When I did neuropathology at a major institution, we froze all frozen sections in an isopentane slurry cooled with LN2. We waited for the OCT to warm to cryostate temps before cutting. If there was time pressure from the surgeons, I used my thumb to warm more quickly, until sections stopped falling apart. We got minimum freeze artifact with this method. For research we used homogenized brain (brain paste) instead of OCT which gave better sections as there was not a change in physical properties you get with OCT vs brain. Bill Blank, MD At 10:40 AM -0400 8/9/10, Della Speranza, Vinnie wrote: >I'm guessing that liquid nitrogen or dry ice temperature is too cold for sectioning OCT. >OCT cuts well down to about -25 degrees C. >Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the same temp range at LN2 > >You will want to give the OCT blocks the opportunity to "warm up" to cryostat temperature before attempting to section them. Leave your frozen blocks in the cryostat for 30-60 minutes before sectioning to allow them to come to optimum temperature From aaperghis <@t> uspath.com Mon Aug 9 11:00:29 2010 From: aaperghis <@t> uspath.com (Adrienne Aperghis Kavanagh) Date: Mon Aug 9 11:00:53 2010 Subject: [Histonet] Precipitate in Processor In-Reply-To: <17053594.318127.1281369484997.JavaMail.root@mail3d.brinkster.com> Message-ID: <12063462.318193.1281369629316.JavaMail.root@mail3d.brinkster.com> Hello Everyone, Has anyone ever seen a (salt?) precipitate in their alcohols following formalin? While changing the processor this morning, I noticed a precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol). It is white and grainy. The alcohols were otherwise unaffected. We are using a 10% NBF containing: Formaldehyde Water Sodium Phosphate, monobasic Sodium Phosphate, dibasic Methanol And our alcohols are all reagent grade. Any help would be very much appreciated! Thank you in advance! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 From brandihiggins <@t> gmail.com Mon Aug 9 11:13:18 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Mon Aug 9 11:13:22 2010 Subject: [Histonet] Precipitate in Processor In-Reply-To: <12063462.318193.1281369629316.JavaMail.root@mail3d.brinkster.com> References: <17053594.318127.1281369484997.JavaMail.root@mail3d.brinkster.com> <12063462.318193.1281369629316.JavaMail.root@mail3d.brinkster.com> Message-ID: Phosphate buffered formalin followed by concentrated alcohol will produce these phosphate salts. To prevent this, formalin should be followed by alcohol of 70% or less. Also, when you change your processing solutions, you can do a water flush (we do first 4 solutions - 2 formalin 2 alcohol) to dissolve any salts that may be built up in the lines. Brandi Higgins HT(ASCP), BS On Mon, Aug 9, 2010 at 12:00 PM, Adrienne Aperghis Kavanagh < aaperghis@uspath.com> wrote: > Hello Everyone, > > Has anyone ever seen a (salt?) precipitate in their alcohols following > formalin? While changing the processor this morning, I noticed a > precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol). It > is white and grainy. The alcohols were otherwise unaffected. > > We are using a 10% NBF containing: > Formaldehyde > Water > Sodium Phosphate, monobasic > Sodium Phosphate, dibasic > Methanol > > And our alcohols are all reagent grade. > > Any help would be very much appreciated! Thank you in advance! > > > Adrienne Aperghis Kavanagh > US PATH > 30 W. Century Road > Suite 255 > Paramus NJ 07652 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Mon Aug 9 11:18:53 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Aug 9 11:18:56 2010 Subject: [Histonet] RELIA Solutions Hot Histology Job Alert!! Histotech needed in Indianapolis area. Message-ID: Hi Histonetters! I want to put the word out on another position I am very excited about. I am working with a hospital in the Indianapolis area who is in need of a histotech with at least 5 years of experience for a permanent day shift position. My client offers excellent pay, benefits and relocation assistance. If you or anyone you know might be interested please contact me at relia1@earthlink.net or toll free at 866-607-3542. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From gu.lang <@t> gmx.at Mon Aug 9 11:22:04 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Aug 9 11:22:10 2010 Subject: AW: [Histonet] Re: [Special Stains] Masson's Trichrome In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24532@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24532@PHSXMB30.partners.org> Message-ID: We don't perfom Masson Trichrome but the one-step Gomori version and another rather similar trichrome called SFOG. I use Bouins for one year, better said, when the reagens in the coplin jar goes low, I fill it up again. Our selfmade SFOG solution is used even longer than one year. And the commercial Gomori-solution is used for a half year. Weigert's is made once a week, but the results show that the older solution doesn't work as well. Since nuclei aren't really important in the trichromes, it doesn't matter that much. It's important, that the pH is correct and low enough, to render the solution stable. Our numbers of stained trichromes are about 150 to 200 per year. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sherwood, Margaret Gesendet: Montag, 09. August 2010 16:33 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: [Special Stains] Masson's Trichrome To all: We keep having the same discussion re: special stains. How long can you use them before discarding? I am specifically referring to the stains used in Masson's Trichrome. We are a research lab and, therefore, don't run the volume that most labs do. We have found, for instance, that we can use the Weigert's Hematoxylin (A&B) more than once, with filtering (even though it is stated it should be made fresh). We also use Bouin's Fix, Biebrich Scarlet and Aniline Blue more than once. Is there a "set rule" when we should discard these solutions (i.e. after 5X use or 1 month)? I would appreciate some feedback from the experts! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jon.St.Onge <@t> dako.com Mon Aug 9 11:23:22 2010 From: Jon.St.Onge <@t> dako.com (Jon St.Onge) Date: Mon Aug 9 11:23:36 2010 Subject: [Histonet] Precipitate in Processor In-Reply-To: <12063462.318193.1281369629316.JavaMail.root@mail3d.brinkster.com> References: <17053594.318127.1281369484997.JavaMail.root@mail3d.brinkster.com> <12063462.318193.1281369629316.JavaMail.root@mail3d.brinkster.com> Message-ID: <8B07D141BCDE434285DC12B3290E3FB304EE5635@exbackca.caus.dako.net> That is a phosphate precipitate most likely from your 10%NBF. Try going into a 70% ETOH first followed by higher concentrations. To see for yourself pour 10%NBF into graded alcohols- Precipitate is easy to see with 100% ETOH Jon Henry St. Onge Dako North America Quality Control Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Aperghis Kavanagh Sent: Monday, August 09, 2010 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Precipitate in Processor Hello Everyone, Has anyone ever seen a (salt?) precipitate in their alcohols following formalin? While changing the processor this morning, I noticed a precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol). It is white and grainy. The alcohols were otherwise unaffected. We are using a 10% NBF containing: Formaldehyde Water Sodium Phosphate, monobasic Sodium Phosphate, dibasic Methanol And our alcohols are all reagent grade. Any help would be very much appreciated! Thank you in advance! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 41dmb41 <@t> gmail.com Mon Aug 9 11:25:25 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Mon Aug 9 11:25:49 2010 Subject: [Histonet] Precipitate in Processor In-Reply-To: <12063462.318193.1281369629316.JavaMail.root@mail3d.brinkster.com> References: <17053594.318127.1281369484997.JavaMail.root@mail3d.brinkster.com> <12063462.318193.1281369629316.JavaMail.root@mail3d.brinkster.com> Message-ID: My guess is that either your 70% wasn't made up properly and was a higher concentration or it's been so long since you've changed the solution that the water is fully saturated with the formalin salts. If it becomes a regular problem, you might consider reducing your first alcohol's concentration to 60% or even 50%. Good luck! Drew On Mon, Aug 9, 2010 at 12:00, Adrienne Aperghis Kavanagh < aaperghis@uspath.com> wrote: > Hello Everyone, > > Has anyone ever seen a (salt?) precipitate in their alcohols following > formalin? While changing the processor this morning, I noticed a > precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol). It > is white and grainy. The alcohols were otherwise unaffected. > > We are using a 10% NBF containing: > Formaldehyde > Water > Sodium Phosphate, monobasic > Sodium Phosphate, dibasic > Methanol > > And our alcohols are all reagent grade. > > Any help would be very much appreciated! Thank you in advance! > > > Adrienne Aperghis Kavanagh > US PATH > 30 W. Century Road > Suite 255 > Paramus NJ 07652 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MSHERWOOD <@t> PARTNERS.ORG Mon Aug 9 11:32:41 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Aug 9 11:32:47 2010 Subject: [Histonet] Re: Stability of Special Stains Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24536@PHSXMB30.partners.org> I want to thank everyone who answered my inquiry into the stability of special stains. And a special thanks to Rena Fail, for the link to the University of Rochester's Special Stains manual. It was very helpful. You have answered my questions. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From arvidsonkristen <@t> yahoo.com Mon Aug 9 12:00:25 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Mon Aug 9 12:00:30 2010 Subject: [Histonet] Reagent Grade Alcohol Brands Message-ID: <759312.99121.qm@web65704.mail.ac4.yahoo.com> Hello All, What brands of reagent grade ETOH is everyone using??? Leica is having some manufacturing issues...need some ASAP!! From aaperghis <@t> uspath.com Mon Aug 9 12:14:02 2010 From: aaperghis <@t> uspath.com (Adrienne Aperghis Kavanagh) Date: Mon Aug 9 12:14:12 2010 Subject: [Histonet] Re: Precipitate in Processor In-Reply-To: <7691923.318614.1281373989695.JavaMail.root@mail3d.brinkster.com> Message-ID: <20990776.318616.1281374042694.JavaMail.root@mail3d.brinkster.com> Thank you all for your quick and helpful responses! I find it strange that we haven't encountered this problem until today, since we have been using the same NBF and alcohols... We usually do the processor hot water flush weekly, on the 1st four stations. I will flush the 1st seven stations and will do it twice to be sure we get out any leftover precipitate. In addition to the flush, I will change the 70% alcohol daily until we receive in another formalin. Thanks again all! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Monday, August 9, 2010 1:03:43 PM Subject: Histonet Digest, Vol 81, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. CA4DF32ED505D94BB55E95487D8E984104FD48@DOAISD5205.state.mt.ads (Andrew Burgeson) 2. Re tunel staining (Steven Weston) 3. Re: [Special Stains] Masson's Trichrome (Sherwood, Margaret ) 4. RE: friable or crumbly O.C.T. (Della Speranza, Vinnie) 5. Re: friable or crumbly O.C.T. (jsjurczak@comcast.net) 6. Anti-IDO (indolamine 2,3-dioxygenase) (Mark Tarango) 7. Re: Re: [Special Stains] Masson's Trichrome (Rena Fail) 8. RELIA Histology Job Alert 8-09-10 Histotech needed in Charlotte, NC (Pam Barker) 9. RE: friable or crumbly O.C.T. (Bill B.) 10. Precipitate in Processor (Adrienne Aperghis Kavanagh) 11. Re: Precipitate in Processor (Brandi Higgins) 12. RELIA Solutions Hot Histology Job Alert!! Histotech needed in Indianapolis area. (Pam Barker) 13. AW: [Histonet] Re: [Special Stains] Masson's Trichrome (Gudrun Lang) 14. RE: Precipitate in Processor (Jon St.Onge) 15. Re: Precipitate in Processor (Drew Meyer) 16. Re: Stability of Special Stains (Sherwood, Margaret ) ---------------------------------------------------------------------- Message: 1 Date: Sun, 08 Aug 2010 13:10:54 -0400 From: "Andrew Burgeson" Subject: [Histonet] CA4DF32ED505D94BB55E95487D8E984104FD48@DOAISD5205.state.mt.ads To: Histonet@lists.utsouthwestern.edu Message-ID: <4c5ee51e.73.527e.1030580047@siscom.net> Content-Type: text/plain; charset="iso-8859-1" I am wondering how much agitation is required in order to achieve the clearing of paraffin from the tissue and slide. IHC systems obviously use adhesive or + slides, aiding in tissue adherence. Too much agitation might take take tissue off. And what about nail fragments or hard tissues in general? Will they survive? I have an article and info regarding this type of deparaffinization and will try to get it into an e-mail. My sense is that it could very well be less efficient and time consuming and that depending on how it is done, could yield very different results. I still think that "there's nothing like xylene!!!" But obviously soap and water doesnt hurt your liver.... Interesting method I would like to hear more about people's experiences. ------------------------------ Message: 2 Date: Mon, 9 Aug 2010 14:32:52 +1000 From: Steven Weston Subject: [Histonet] Re tunel staining To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="big5" I recently had an enquiry from one of our post docs who was looking at TUNEL staining and came across this reference in biochemica no 4 (1997)titled ??Fixation of Tissue Sections for TUNEL Combined with Staining for Thymic Epithelial Cell Marker?? You should be able to google it and find the article. Basically it said that after fixation with PFA to improve staining you need to treat with triton and sodium citrate in the cold to expose the antigen again. Below is the suggested protocol for thin cryostat sections. I??m sure it could be modified for thick sections. 1. Cut 4 ?gm thin cryosections and mount on polylysine-coated glass slides. 2. Air dry overnight at room temperature and freeze foil- wrapped slides at ?V20?XC until use. 3. Apply frozen glass slides directly into a container with 1% buffered paraformaldehyde for 30 min at RT. 4. Rinse swiftly in PBS and immerse in a solution of 1% Triton X100 (v/v) and 1% sodium citrate (w/v) for 2 min at 4?XC. 5. Wash in PBS and incubate with 50 ?gl TUNEL reaction mixture (TdT solution with dUTP-FITC solution, 1+9). Convert to enzyme label if desired. 6. Wash with PBS and block with dilution of adequate normal serum. 10. Proceed with desired immunohistochemical labeling. Regards Steve Weston Senior technical officer Menzies Research Institute Hobart Tasmania ------------------------------ Message: 3 Date: Mon, 9 Aug 2010 10:32:48 -0400 From: "Sherwood, Margaret " Subject: [Histonet] Re: [Special Stains] Masson's Trichrome To: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24532@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" To all: We keep having the same discussion re: special stains. How long can you use them before discarding? I am specifically referring to the stains used in Masson's Trichrome. We are a research lab and, therefore, don't run the volume that most labs do. We have found, for instance, that we can use the Weigert's Hematoxylin (A&B) more than once, with filtering (even though it is stated it should be made fresh). We also use Bouin's Fix, Biebrich Scarlet and Aniline Blue more than once. Is there a "set rule" when we should discard these solutions (i.e. after 5X use or 1 month)? I would appreciate some feedback from the experts! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 4 Date: Mon, 9 Aug 2010 10:40:03 -0400 From: "Della Speranza, Vinnie" Subject: RE: [Histonet] friable or crumbly O.C.T. To: "'Bruce W Brodersen'" , "histonet@pathology.swmed.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I'm guessing that liquid nitrogen or dry ice temperature is too cold for sectioning OCT. OCT cuts well down to about -25 degrees C. Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the same temp range at LN2 You will want to give the OCT blocks the opportunity to "warm up" to cryostat temperature before attempting to section them. Leave your frozen blocks in the cryostat for 30-60 minutes before sectioning to allow them to come to optimum temperature Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce W Brodersen Sent: Friday, August 06, 2010 2:26 PM To: histonet@pathology.swmed.edu Subject: [Histonet] friable or crumbly O.C.T. Anyone have an explanation as to why OCT would be friable or crumbly for sectioning? Here's how it was used. Thanks. "We held the plastic 'tray' with the tissue in the compound just over the liquid nitro for 30sec-1min, until it was opaque and white (frozen) and then dipped the tray into the liquid nitro for 20-30sec., placed in small bags and then into a cooler with dry ice until shipping." Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 9 Aug 2010 14:49:11 +0000 (UTC) From: jsjurczak@comcast.net Subject: Re: [Histonet] friable or crumbly O.C.T. To: "Della Speranza, Vinnie" Cc: histonet@pathology.swmed.edu Message-ID: <2100844269.41947.1281365351046.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Content-Type: text/plain; charset=utf-8 There is an OCT for lower temperatures. ----- Original Message ----- From: "Della Speranza, Vinnie" To: "Bruce W Brodersen" , histonet@pathology.swmed.edu Sent: Monday, August 9, 2010 9:40:03 AM Subject: RE: [Histonet] friable or crumbly O.C.T. I'm guessing that liquid nitrogen or dry ice temperature is too cold for sectioning OCT. OCT cuts well down to about -25 degrees C. Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the same temp range at LN2 You will want to give the OCT blocks the opportunity to "warm up" to cryostat temperature before attempting to section them. Leave your frozen blocks in the cryostat for 30-60 minutes before sectioning to allow them to come to optimum temperature Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue ??Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 ?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce W Brodersen Sent: Friday, August 06, 2010 2:26 PM To: histonet@pathology.swmed.edu Subject: [Histonet] friable or crumbly O.C.T. Anyone have an explanation as to why OCT would be friable or crumbly for sectioning? ??Here's how it was used. Thanks. "We held the plastic 'tray' with the tissue in the compound just over the liquid nitro for 30sec-1min, until it was opaque and white (frozen) and then dipped the tray into the liquid nitro for 20-30sec., placed in small bags and then into a cooler with dry ice until shipping." Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE ??68583-0907 voice (402) 472-1434 FAX (402 472-3094_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 9 Aug 2010 07:53:48 -0700 From: Mark Tarango Subject: [Histonet] Anti-IDO (indolamine 2,3-dioxygenase) To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonet, Does anyone use an Anti-IDO on FFPE tissues? Would you please let me know where you purchase it? Thank you, Mark Tarango ------------------------------ Message: 7 Date: Mon, 9 Aug 2010 08:24:01 -0700 (PDT) From: Rena Fail Subject: Re: [Histonet] Re: [Special Stains] Masson's Trichrome To: "Sherwood, Margaret" , histonet@lists.utsouthwestern.edu Message-ID: <426670.14254.qm@web180808.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 It depends on the number of slides as well as time on the shelf and a little common sense.Your stock solutions have a longer shelf life than?your working. ?I worked in a lab that perfomed a large number of Masson's by hand( until purchasing the Artisan)? We changed the solutions once a week to insure consistent staining.? If you perform these stains only twice a month, your results will be more consistant if you make the Weigert's fresh? and watch the level of your aniline blue, evaporation will make it more concentrated.? On the other hand a large volume of slides will weaken the?working solutions. following is a link that lists some guidelines for storing stock solutions Rena Fail www.urmc.rochester.edu/.../StainsManual/STABILITYOFSPECIALSTAININGSOLUTIONS.html?...- Cached ----- Original Message ---- From: "Sherwood, Margaret" To: histonet@lists.utsouthwestern.edu Sent: Mon, August 9, 2010 10:32:48 AM Subject: [Histonet] Re: [Special Stains] Masson's Trichrome To all: We keep having the same discussion re:? special stains.? How long can you use them before discarding?? I am specifically referring to the stains used in Masson's Trichrome. We are a research lab and, therefore, don't run the volume that most labs do. We have found, for instance, that we can use the Weigert's Hematoxylin (A&B) more than once, with filtering (even though it is stated it should be made fresh).? We also use Bouin's Fix, Biebrich Scarlet and Aniline Blue more than once.? Is there a "set rule" when we should discard these solutions (i.e. after 5X use or 1 month)? I would appreciate some feedback from the experts! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 9 Aug 2010 11:50:21 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Histology Job Alert 8-09-10 Histotech needed in Charlotte, NC To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters!! I hope everyone had a great weekend. I have an exciting new opportunity to tell you about. I am working with a premier client located Charlotte, NC. This is a part time (32 hours per week) permanent position in a full service histology lab. My client is looking for an ASCP certified histotech with at least 2 years of histology experience must be able to meet CLIA requirements to do grossing. (My client does mainly simple grossing, mainly biopsies and excisions). My client offers excellent pay and benefits. If you or anyone you know would like more information please contact me at 866-607-3542 or relia1@earthlink.net Have a Great Day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 9 Date: Mon, 9 Aug 2010 10:55:40 -0500 From: "Bill B." Subject: RE: [Histonet] friable or crumbly O.C.T. To: "histonet@pathology.swmed.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I will 2nd this. When I did neuropathology at a major institution, we froze all frozen sections in an isopentane slurry cooled with LN2. We waited for the OCT to warm to cryostate temps before cutting. If there was time pressure from the surgeons, I used my thumb to warm more quickly, until sections stopped falling apart. We got minimum freeze artifact with this method. For research we used homogenized brain (brain paste) instead of OCT which gave better sections as there was not a change in physical properties you get with OCT vs brain. Bill Blank, MD At 10:40 AM -0400 8/9/10, Della Speranza, Vinnie wrote: >I'm guessing that liquid nitrogen or dry ice temperature is too cold >for sectioning OCT. >OCT cuts well down to about -25 degrees C. >Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the >same temp range at LN2 > >You will want to give the OCT blocks the opportunity to "warm up" to >cryostat temperature before attempting to section them. Leave your >frozen blocks in the cryostat for 30-60 minutes before sectioning to >allow them to come to optimum temperature ------------------------------ Message: 10 Date: Mon, 9 Aug 2010 09:00:29 -0700 (MST) From: Adrienne Aperghis Kavanagh Subject: [Histonet] Precipitate in Processor To: histonet@lists.utsouthwestern.edu Message-ID: <12063462.318193.1281369629316.JavaMail.root@mail3d.brinkster.com> Content-Type: text/plain; charset=utf-8 Hello Everyone, Has anyone ever seen a (salt?) precipitate in their alcohols following formalin? While changing the processor this morning, I noticed a precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol). It is white and grainy. The alcohols were otherwise unaffected. We are using a 10% NBF containing: Formaldehyde Water Sodium Phosphate, monobasic Sodium Phosphate, dibasic Methanol And our alcohols are all reagent grade. Any help would be very much appreciated! Thank you in advance! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ------------------------------ Message: 11 Date: Mon, 9 Aug 2010 12:13:18 -0400 From: Brandi Higgins Subject: Re: [Histonet] Precipitate in Processor To: Adrienne Aperghis Kavanagh Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Phosphate buffered formalin followed by concentrated alcohol will produce these phosphate salts. To prevent this, formalin should be followed by alcohol of 70% or less. Also, when you change your processing solutions, you can do a water flush (we do first 4 solutions - 2 formalin 2 alcohol) to dissolve any salts that may be built up in the lines. Brandi Higgins HT(ASCP), BS On Mon, Aug 9, 2010 at 12:00 PM, Adrienne Aperghis Kavanagh < aaperghis@uspath.com> wrote: > Hello Everyone, > > Has anyone ever seen a (salt?) precipitate in their alcohols following > formalin? While changing the processor this morning, I noticed a > precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% > alcohol). It > is white and grainy. The alcohols were otherwise unaffected. > > We are using a 10% NBF containing: > Formaldehyde Water > Sodium Phosphate, monobasic > Sodium Phosphate, dibasic > Methanol > > And our alcohols are all reagent grade. > > Any help would be very much appreciated! Thank you in advance! > > > Adrienne Aperghis Kavanagh > US PATH > 30 W. Century Road > Suite 255 > Paramus NJ 07652 > > > _______________________________________________ Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Mon, 9 Aug 2010 12:18:53 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Solutions Hot Histology Job Alert!! Histotech needed in Indianapolis area. To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters! I want to put the word out on another position I am very excited about. I am working with a hospital in the Indianapolis area who is in need of a histotech with at least 5 years of experience for a permanent day shift position. My client offers excellent pay, benefits and relocation assistance. If you or anyone you know might be interested please contact me at relia1@earthlink.net or toll free at 866-607-3542. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 13 Date: Mon, 9 Aug 2010 18:22:04 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: [Special Stains] Masson's Trichrome To: "'Sherwood, Margaret '" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" We don't perfom Masson Trichrome but the one-step Gomori version and another rather similar trichrome called SFOG. I use Bouins for one year, better said, when the reagens in the coplin jar goes low, I fill it up again. Our selfmade SFOG solution is used even longer than one year. And the commercial Gomori-solution is used for a half year. Weigert's is made once a week, but the results show that the older solution doesn't work as well. Since nuclei aren't really important in the trichromes, it doesn't matter that much. It's important, that the pH is correct and low enough, to render the solution stable. Our numbers of stained trichromes are about 150 to 200 per year. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sherwood, Margaret Gesendet: Montag, 09. August 2010 16:33 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: [Special Stains] Masson's Trichrome To all: We keep having the same discussion re: special stains. How long can you use them before discarding? I am specifically referring to the stains used in Masson's Trichrome. We are a research lab and, therefore, don't run the volume that most labs do. We have found, for instance, that we can use the Weigert's Hematoxylin (A&B) more than once, with filtering (even though it is stated it should be made fresh). We also use Bouin's Fix, Biebrich Scarlet and Aniline Blue more than once. Is there a "set rule" when we should discard these solutions (i.e. after 5X use or 1 month)? I would appreciate some feedback from the experts! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 9 Aug 2010 09:23:22 -0700 From: "Jon St.Onge" Subject: RE: [Histonet] Precipitate in Processor To: "Adrienne Aperghis Kavanagh" , Message-ID: <8B07D141BCDE434285DC12B3290E3FB304EE5635@exbackca.caus.dako.net> Content-Type: text/plain; charset="UTF-8" That is a phosphate precipitate most likely from your 10%NBF. Try going into a 70% ETOH first followed by higher concentrations. To see for yourself pour 10%NBF into graded alcohols- Precipitate is easy to see with 100% ETOH Jon Henry St. Onge Dako North America Quality Control Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Aperghis Kavanagh Sent: Monday, August 09, 2010 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Precipitate in Processor Hello Everyone, Has anyone ever seen a (salt?) precipitate in their alcohols following formalin? While changing the processor this morning, I noticed a precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol). It is white and grainy. The alcohols were otherwise unaffected. We are using a 10% NBF containing: Formaldehyde Water Sodium Phosphate, monobasic Sodium Phosphate, dibasic Methanol And our alcohols are all reagent grade. Any help would be very much appreciated! Thank you in advance! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Mon, 9 Aug 2010 12:25:25 -0400 From: Drew Meyer <41dmb41@gmail.com> Subject: Re: [Histonet] Precipitate in Processor To: Adrienne Aperghis Kavanagh Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 My guess is that either your 70% wasn't made up properly and was a higher concentration or it's been so long since you've changed the solution that the water is fully saturated with the formalin salts. If it becomes a regular problem, you might consider reducing your first alcohol's concentration to 60% or even 50%. Good luck! Drew On Mon, Aug 9, 2010 at 12:00, Adrienne Aperghis Kavanagh < aaperghis@uspath.com> wrote: > Hello Everyone, > > Has anyone ever seen a (salt?) precipitate in their alcohols following > formalin? While changing the processor this morning, I noticed a > precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% > alcohol). It > is white and grainy. The alcohols were otherwise unaffected. > > We are using a 10% NBF containing: > Formaldehyde Water > Sodium Phosphate, monobasic > Sodium Phosphate, dibasic > Methanol > > And our alcohols are all reagent grade. > > Any help would be very much appreciated! Thank you in advance! > > > Adrienne Aperghis Kavanagh > US PATH > 30 W. Century Road > Suite 255 > Paramus NJ 07652 > > > _______________________________________________ Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 16 Date: Mon, 9 Aug 2010 12:32:41 -0400 From: "Sherwood, Margaret " Subject: [Histonet] Re: Stability of Special Stains To: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24536@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" I want to thank everyone who answered my inquiry into the stability of special stains. And a special thanks to Rena Fail, for the link to the University of Rochester's Special Stains manual. It was very helpful. You have answered my questions. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 9 *************************************** From lucy.zong <@t> gmail.com Mon Aug 9 12:25:10 2010 From: lucy.zong <@t> gmail.com (Lucy Zong) Date: Mon Aug 9 12:25:14 2010 Subject: [Histonet] Leica 2125 Message-ID: Have little Fiscal year money left . Looking for usd Leica 2125 cheep. From Jackie.O'Connor <@t> abbott.com Mon Aug 9 12:29:34 2010 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Aug 9 12:29:39 2010 Subject: [Histonet] Reagent Grade Alcohol Brands In-Reply-To: <759312.99121.qm@web65704.mail.ac4.yahoo.com> References: <759312.99121.qm@web65704.mail.ac4.yahoo.com> Message-ID: What kind of issues? I'm considering switching over to all Surgipath (Leica) products. I'd like to hear more of this. From: kristen arvidson To: histonet Date: 08/09/2010 12:05 PM Subject: [Histonet] Reagent Grade Alcohol Brands Sent by: histonet-bounces@lists.utsouthwestern.edu Hello All, What brands of reagent grade ETOH is everyone using?? Leica is having some manufacturing issues...need some ASAP!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Aug 9 13:33:14 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Aug 9 13:33:18 2010 Subject: [Histonet] Reagent Grade Alcohol Brands In-Reply-To: <759312.99121.qm@web65704.mail.ac4.yahoo.com> References: <759312.99121.qm@web65704.mail.ac4.yahoo.com> Message-ID: <3734.165.124.15.208.1281378794.squirrel@merle.it.northwestern.edu> We use Protocol from Fisher. Ask them for pricing. We get 70,80,95 and 100% from them. No problems. Bernice > Hello All, > What brands of reagent grade ETOH is everyone using??? Leica is having some > manufacturing issues...need some ASAP!! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Bernice Frederick HTL (ASCP) Pathology Core Facility Robert H Lurie Cancer Center Northwestern University Olson Room 8421 Chicago Il 60611 312-503-3723 From aaperghis <@t> uspath.com Mon Aug 9 13:46:27 2010 From: aaperghis <@t> uspath.com (Adrienne Aperghis Kavanagh) Date: Mon Aug 9 13:46:48 2010 Subject: [Histonet] p63 from Ventana In-Reply-To: <24915043.319070.1281379460728.JavaMail.root@mail3d.brinkster.com> Message-ID: <25616992.319074.1281379587856.JavaMail.root@mail3d.brinkster.com> Hi Everyone, We have been using the p63 (BC4A4) antibody from BioCare for the past 5 years with great results. We use it as a component on our triple stain for prostate. I have been seeing ads on the Ventana website for their p63 and was wondering if anyone else had used their p63 and in what immuno? Any feedback would be greatly appreciated!!! Thanks again! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 From marktarango <@t> gmail.com Mon Aug 9 13:46:49 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Aug 9 13:46:55 2010 Subject: [Histonet] Re: Anti-IDO (indolamine 2,3-dioxygenase) In-Reply-To: References: Message-ID: Here I am answering my own question. I just remembered where I used to get this antibody. It's from Millipore/Chemicon. Mark On Mon, Aug 9, 2010 at 7:53 AM, Mark Tarango wrote: > Hi Histonet, > > Does anyone use an Anti-IDO on FFPE tissues? Would you please let me know > where you purchase it? > > Thank you, > Mark Tarango > From Ronald.Houston <@t> nationwidechildrens.org Mon Aug 9 14:08:30 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Aug 9 14:08:38 2010 Subject: [Histonet] correct CPT code Message-ID: How do others code analysis of renal bxs and cilia bxs for specimen adequacy; and can this be performed by a PA and/or HT who is CLIA qualified to gross? I know of 88172, but this is for adequacy of FNA specimens; can you use 88329, pathology consultation during surgery? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Bill.Tench <@t> pph.org Mon Aug 9 14:17:24 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Mon Aug 9 14:17:31 2010 Subject: [Histonet] correct CPT code In-Reply-To: References: Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5506@MAIL1.pph.local> You need to be more specific about what it is you are doing. If you are looking at imprints or smears "intraoperatively" then the correct code is 88333. I would say that neither a PA or HT is qualified for this job, but a cytotech is. If anyone other than a pathologist does it, however, you cannot charge for it. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Monday, August 09, 2010 12:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] correct CPT code How do others code analysis of renal bxs and cilia bxs for specimen adequacy; and can this be performed by a PA and/or HT who is CLIA qualified to gross? I know of 88172, but this is for adequacy of FNA specimens; can you use 88329, pathology consultation during surgery? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- From mpence <@t> grhs.net Mon Aug 9 14:52:51 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Aug 9 14:53:54 2010 Subject: [Histonet] correct CPT code In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A5506@MAIL1.pph.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3E95@is-e2k3.grhs.net> This is how I view this also. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Monday, August 09, 2010 2:17 PM To: Houston, Ronald Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] correct CPT code You need to be more specific about what it is you are doing. If you are looking at imprints or smears "intraoperatively" then the correct code is 88333. I would say that neither a PA or HT is qualified for this job, but a cytotech is. If anyone other than a pathologist does it, however, you cannot charge for it. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Monday, August 09, 2010 12:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] correct CPT code How do others code analysis of renal bxs and cilia bxs for specimen adequacy; and can this be performed by a PA and/or HT who is CLIA qualified to gross? I know of 88172, but this is for adequacy of FNA specimens; can you use 88329, pathology consultation during surgery? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Mon Aug 9 14:57:21 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Aug 9 14:57:31 2010 Subject: [Histonet] correct CPT code In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A5506@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A5506@MAIL1.pph.local> Message-ID: Bill, I'm specifically referring to visualization of a renal biopsy under a dissecting scope to see if the specimen is adequate for analysis (i.e. are there glomeruli present?) and examining a ciliary biopsy, again under dissecting microscope, to check for the presence of motile cilia Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: Tench, Bill [mailto:Bill.Tench@pph.org] Sent: Monday, August 09, 2010 3:17 PM To: Houston, Ronald Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] correct CPT code You need to be more specific about what it is you are doing. If you are looking at imprints or smears "intraoperatively" then the correct code is 88333. I would say that neither a PA or HT is qualified for this job, but a cytotech is. If anyone other than a pathologist does it, however, you cannot charge for it. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Monday, August 09, 2010 12:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] correct CPT code How do others code analysis of renal bxs and cilia bxs for specimen adequacy; and can this be performed by a PA and/or HT who is CLIA qualified to gross? I know of 88172, but this is for adequacy of FNA specimens; can you use 88329, pathology consultation during surgery? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- From eridana <@t> cox.net Mon Aug 9 15:26:22 2010 From: eridana <@t> cox.net (Eridana) Date: Mon Aug 9 15:26:27 2010 Subject: [Histonet] Flat mount eye sections In-Reply-To: <20100805170214.IUSB10137.fed1rmmtai103.cox.net@fed1rmimpi05.cox.net> Message-ID: <20100809162622.CHQGH.370203.imail@fed1rmwml45> I have done flat retina sections- which may be what you are being asked to do which is a very specialized procedure even for most research labs. Do you have access to a sliding microtome with a freezing stage? Otherwise I do not know any way to do this. If you are doing flat retina (I have done birds and rats- the rats are very difficult and require a dissecting scope to even prepare them). I did this in a lab 15 years ago but now that I am older and do not see so well, I would use a scope for any species. For birds or rodents, perfuse the animal usually 4%PFA, remove the eye, excise the cornea, iris and lens and being carefully not disturbing the retina remove the vitreous humor and post fixed in 4% at 4oC overnight Transfer to 30% sucrose in PBS at least another 24 hours Dissect the retina from the eyecup and trimmed it with a fresh scalpel blade to flatten it out. I cannot remember the correct term, but pigeon and chicken eyes have a comb structure inside their eyes that must be removed before flattening. It usually took about 4 cuts of varying sizes to get a flat sample. Looks kind of like an uneven flower with cuts from the center out. On a few eyes they had to be cut into 2 pieces to get them flat. On a sliding microtome with freezing stage (or with dry ice on either end of the platform, build a 30% sucrose platform bigger than the eye to be sectioned Then with the blade in place, shave down the platform to make a flat place big enough to mount the retina. Remove the blade for safety reasons for mounting of the retina. Use a wooden block that is super flat covered with saran wrap. Very carefully on one side so there were no wrinkles in the wrap, place the inside of the eye against the saran wrap (with no liquid or it will slide off) and very carefully but quickly wipe the retina onto the prepared platform and let it freeze. Replace the blade from and drop the stage or raise the blade (depending on the microtome ) and carefully section the retina. On a pigeon we usually got at best 3 30u sections but usually did not get the retina quite flat and got 4 sections. The sections are collected in PBS and stained in micro centrifuge tubes for various antibodies then mounted on slides after staining.. Good luck Donna Harclerode HT,HTL,QIHC (ASCP),SLS Histology Core Manager UCSD, Dept of Pathology 9500 Gillman Drive BSB 2009 San Diego, CA 92093 858 534 7438 Date: Wed, 04 Aug 2010 10:57:02 -0700 From: Subject: [Histonet] Flat Mount To: histonet@lists.utsouthwestern.edu Hello all, I am being asked to do immunostaining on fla eyes. First, what is a flat mount and does an on how to make one? Second, what is the methodol stains on these? Do you have to fix them, cut them, done this and need some help. Thanks guys!! Sarah Goebel, B.A., HT (ASCP) Histotechnician [DEL: XBiot 8201 East Riverside Dr. Austin, Texas From marktarango <@t> gmail.com Mon Aug 9 16:04:44 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Aug 9 16:04:51 2010 Subject: [Histonet] p63 from Ventana In-Reply-To: <25616992.319074.1281379587856.JavaMail.root@mail3d.brinkster.com> References: <24915043.319070.1281379460728.JavaMail.root@mail3d.brinkster.com> <25616992.319074.1281379587856.JavaMail.root@mail3d.brinkster.com> Message-ID: I've been told by a Biocare Salesperson that the BC4A4 clone is the same exact clone as 4A4. The BC in front of 4A4 just means Biocare. I don't think that Ventana sells a concentrate of this antibody. You'd want a concentrate for your PIN4 so you're probably better off sticking with your current antibody. Mark On Mon, Aug 9, 2010 at 11:46 AM, Adrienne Aperghis Kavanagh < aaperghis@uspath.com> wrote: > Hi Everyone, > > We have been using the p63 (BC4A4) antibody from BioCare for the past 5 > years with great results. We use it as a component on our triple stain for > prostate. I have been seeing ads on the Ventana website for their p63 and > was wondering if anyone else had used their p63 and in what immuno? Any > feedback would be greatly appreciated!!! > > Thanks again! > > > > Adrienne Aperghis Kavanagh > US PATH > 30 W. Century Road > Suite 255 > Paramus NJ 07652 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From becky.garrison <@t> jax.ufl.edu Mon Aug 9 16:58:55 2010 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Mon Aug 9 16:58:59 2010 Subject: [Histonet] closure of EM lab Message-ID: We are closing our EM service and have the following equipment to surplus: Philips CM 100 Electron Microscope w/ Haskris Water chiller and Jun Air Compressor. Ted Pella Nitrogen Burst Negative Developing Tank Durst Laborator S-45 EM Enlarger w/ lens for 35 mm, 3 x 4 and 2.25 x 2.25 sheet film Dessicator w/ Vacuum Pump BEEM Negative Dryer Oven Penetron Rotary Shaker Sorvall GKM Glass Knife Maker We have a Leica UC6 ultramicrotome that may be surplused; may be able to trade for current model, good rotary microtome. Most of the equipment was purchased in the late 78 / 79 and has been carefully cared for and maintained. The Philips CM 100 was installed in 11/95 and has been under continuous service with FEI / Philips. The Leica microtome is only 2 / 3 years old. Our hospital follows the surplus guidelines established by the Shands Healthcare System. I am trying to get the word out to other EM users and will gladly put you in contact with our surplus co-ordinator. Or work with you to see the equipment which is still set up within our department. Please pass on to others that may have an interest. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager From Bill.Tench <@t> pph.org Mon Aug 9 16:59:36 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Mon Aug 9 16:59:43 2010 Subject: [Histonet] correct CPT code In-Reply-To: References: <2820431BF953BB4DA3E9E1A5882265FD034A5506@MAIL1.pph.local> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5508@MAIL1.pph.local> OK, so that's a different story (and we look at renal cores for glomeruli as well). I believe the correct code for that is 88329--intraoperative consultation. It is not a frozen section so that code, 88331, does not apply. Again, I believe that to be paid, this activity must be performed by a pathologist. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: Houston, Ronald [mailto:Ronald.Houston@nationwidechildrens.org] Sent: Monday, August 09, 2010 12:57 PM To: Tench, Bill Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] correct CPT code Bill, I'm specifically referring to visualization of a renal biopsy under a dissecting scope to see if the specimen is adequate for analysis (i.e. are there glomeruli present?) and examining a ciliary biopsy, again under dissecting microscope, to check for the presence of motile cilia Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: Tench, Bill [mailto:Bill.Tench@pph.org] Sent: Monday, August 09, 2010 3:17 PM To: Houston, Ronald Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] correct CPT code You need to be more specific about what it is you are doing. If you are looking at imprints or smears "intraoperatively" then the correct code is 88333. I would say that neither a PA or HT is qualified for this job, but a cytotech is. If anyone other than a pathologist does it, however, you cannot charge for it. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Monday, August 09, 2010 12:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] correct CPT code How do others code analysis of renal bxs and cilia bxs for specimen adequacy; and can this be performed by a PA and/or HT who is CLIA qualified to gross? I know of 88172, but this is for adequacy of FNA specimens; can you use 88329, pathology consultation during surgery? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. 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For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- From Jessica.Vacca <@t> HCAhealthcare.com Tue Aug 10 07:51:20 2010 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Tue Aug 10 07:51:08 2010 Subject: [Histonet] Scott Nichols-Used to be UsLabs rep Message-ID: <938D716CD445614ABBB817517557B6F4EB5A1518@NADCWPMSGCMS09.hca.corpad.net> I'm looking for Scott Nichols, he used to be with Uslabs awhile back but has since moved on. If you still stay in touch with him can you have him contact me? Thanks Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon, FL 33511 8133571.6410 (office) 813.571.5169 (fax) From Wanda.Smith <@t> HCAhealthcare.com Tue Aug 10 08:00:36 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Tue Aug 10 08:00:25 2010 Subject: [Histonet] Any Other Manual Cassette Printers Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13910018ED@NADCWPMSGCMS03.hca.corpad.net> Good Morning to All, We are demo-ing the Leica/Surgipath manual cassette printer to replace our OLD Surgipath Cassette printer. It is working well for us, but I was curious if there are any other manual cassette printers on the market. Vendors welcome to respond. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From a.thotakura <@t> imperial.ac.uk Tue Aug 10 09:15:34 2010 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Tue Aug 10 09:16:30 2010 Subject: [Histonet] Slide expiry Message-ID: Dear All, I purchased bulk of Slides polysin coated slides and on the box it say they expire by end of this year, Does it really effect ?? Or I don't have to bother. Please let me know. Many Thanks, Anil Kumar. From ltougas <@t> dawsoncollege.qc.ca Tue Aug 10 12:51:19 2010 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Tue Aug 10 12:51:23 2010 Subject: [Histonet] histological process video Message-ID: <7618BC6D53F39149B7B57615C218AADF114F5F35AC@EXCHANGE.ad.dawsoncollege.qc.ca> Good day, I am looking for a video/CD presentation of the whole histological process for the Histotechniques course. Thank you very much in advance for a prompt reply, Liette Tougas Faculty, Medical Laboratory Technology Program Dawson College, Montreal, Canada From Niloofar.Rezvani <@t> usuhs.mil Tue Aug 10 13:16:37 2010 From: Niloofar.Rezvani <@t> usuhs.mil (Niloofar Rezvani) Date: Tue Aug 10 13:17:46 2010 Subject: [Histonet] brain microvessel staining Message-ID: <4C615F64.B59E.002E.0@usuhs.mil> Hi, I appreciate if someone can answer my question. I am going to stain rats' brain microvessels with ER(estrogen receptor) antibody. We know how we can isolate the vessels but this is the first time to stain them. I have the microvessels in PBS 1X and do you know how I can fix them with paraformaldehyde and mount them on the gel-subbed slides? I did 2 ways: 1- mount the fresh microvessels on the slide and wait overnight to get dried and then fix them in 2% PFA for 30 min and after that washing steps and the rest of IHC protocol or 2- fix the vessels with 2% PFA in the tube for 30 min and then mount them on the slides and wait overnight to get dried. Please advise me with any idea, thanks so much, Dr. Niloofar Rezvani,Ph.D,PharmD Postdoctoral Fellow Graduate School of Nursing Uniformed Services University Cell:410.615.9078 Fax:301.295.1707 Email: niloofar.rezvani@usuhs.mil Classification: UNCLASSIFIED Caveats: None Classification: UNCLASSIFIED Caveats: None From MLashus <@t> pathgroup.com Tue Aug 10 15:38:46 2010 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Tue Aug 10 15:38:50 2010 Subject: [Histonet] Tissue Tek SCA Coverslipper Message-ID: <197CD0B02A81F94994A285C59C8AE05C05D29B9207@pgnexchange.pathgroup.com> I am having a problem with my tape coverslipper. The coverslipper is not positioning the tape properly; when the slides are coverslipped, they have a 1mm overhang on the right side of the slide, would anyone know how to adjust this? Thanks, Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From zodiac29 <@t> comcast.net Wed Aug 11 07:46:46 2010 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Wed Aug 11 07:46:49 2010 Subject: [Histonet] Paraffin Message-ID: <1464661975.1354742.1281530806687.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Hello, Just wanted to know if anyone has tried Henry's Wonder Wax Xtreme Paraffin w/DMSO made by Mercedes Medical. What were your expirences with it? Is it comprable to McCormick's Paraplast Xtra? Thanks in advance, Jenny From Valerie.Hannen <@t> parrishmed.com Wed Aug 11 08:54:39 2010 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Wed Aug 11 08:56:03 2010 Subject: [Histonet] Slide label printers Message-ID: <5680DA93771F0C48954CC8D38425E72401AB3512@ISMAIL.parrishmed.local> Hi gang... I am hoping that someone out there can lend me some assistance. We currently are using paper slide labels( 0.875 in by 0.875 in) on our Pathology slides, an old label printer (Genicom Model 1025) and Meditech Magic software. Our dilemma is this... we no longer can find the ink ribbons for our printer. Is there anyone out there who is using the same system? In about 1 1/2 to 2 years, we are going to be changing to a new software (possibly McKesson), so we are not looking to really buy anything expensive at this time. We just really need to continue using the paper labels until we have the change over. Thanks for your help. Valerie Hannen, MLT (ASCP),HTL,SU(FL) Parrish Medical Center Titusville,Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From GauchV <@t> mail.amc.edu Wed Aug 11 12:05:15 2010 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Wed Aug 11 12:05:25 2010 Subject: [Histonet] Ventana ES labels Message-ID: Hi, I am posting this for a colleague of mine....She is in the middle of a project and found out that she can no longer order Ventana ES labels. She wants to know if anyone got rid of their ES stainer but still has some labels in their lab that they are looking to get rid of....or if anyone knows where she might find some labels... Any help would be greatly appreciated. Please respond to my e-mail and I will get the information to her since she is not on the Histonet... Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From neelyk <@t> shands.ufl.edu Wed Aug 11 12:43:30 2010 From: neelyk <@t> shands.ufl.edu (Kendall Neely) Date: Wed Aug 11 12:43:40 2010 Subject: [Histonet] Re: Job Posting- Gainesville, Fl- Shands Hospital In-Reply-To: <05f5d01700028c65@Shands.Local> References: <05f5d01700028c65@Shands.Local> Message-ID: <4C62A901.3024.0059.1@shands.ufl.edu> Position open at Shands Hospital in Gainesville, Fl Histotechnologist, Full-time, day shift http://Shands.org Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 >>> 8/11/2010 1:02 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. histological process video (Liette Tougas) 2. brain microvessel staining (Niloofar Rezvani) 3. Tissue Tek SCA Coverslipper (Mighnon Lashus) 4. Paraffin (zodiac29@comcast.net) 5. Slide label printers (Hannen, Valerie) ---------------------------------------------------------------------- Message: 1 Date: Tue, 10 Aug 2010 13:51:19 -0400 From: Liette Tougas Subject: [Histonet] histological process video To: "histonet@lists.utsouthwestern.edu" Message-ID: <7618BC6D53F39149B7B57615C218AADF114F5F35AC@EXCHANGE.ad.dawsoncollege.qc.ca> Content-Type: text/plain; charset="iso-8859-1" Good day, I am looking for a video/CD presentation of the whole histological process for the Histotechniques course. Thank you very much in advance for a prompt reply, Liette Tougas Faculty, Medical Laboratory Technology Program Dawson College, Montreal, Canada ------------------------------ Message: 2 Date: Tue, 10 Aug 2010 14:16:37 -0400 From: "Niloofar Rezvani" Subject: [Histonet] brain microvessel staining To: Message-ID: <4C615F64.B59E.002E.0@usuhs.mil> Content-Type: text/plain; charset=US-ASCII Hi, I appreciate if someone can answer my question. I am going to stain rats' brain microvessels with ER(estrogen receptor) antibody. We know how we can isolate the vessels but this is the first time to stain them. I have the microvessels in PBS 1X and do you know how I can fix them with paraformaldehyde and mount them on the gel-subbed slides? I did 2 ways: 1- mount the fresh microvessels on the slide and wait overnight to get dried and then fix them in 2% PFA for 30 min and after that washing steps and the rest of IHC protocol or 2- fix the vessels with 2% PFA in the tube for 30 min and then mount them on the slides and wait overnight to get dried. Please advise me with any idea, thanks so much, Dr. Niloofar Rezvani,Ph.D,PharmD Postdoctoral Fellow Graduate School of Nursing Uniformed Services University Cell:410.615.9078 Fax:301.295.1707 Email: niloofar.rezvani@usuhs.mil Classification: UNCLASSIFIED Caveats: None Classification: UNCLASSIFIED Caveats: None ------------------------------ Message: 3 Date: Tue, 10 Aug 2010 15:38:46 -0500 From: Mighnon Lashus Subject: [Histonet] Tissue Tek SCA Coverslipper To: "histonet@lists.utsouthwestern.edu" Message-ID: <197CD0B02A81F94994A285C59C8AE05C05D29B9207@pgnexchange.pathgroup.com> Content-Type: text/plain; charset="us-ascii" I am having a problem with my tape coverslipper. The coverslipper is not positioning the tape properly; when the slides are coverslipped, they have a 1mm overhang on the right side of the slide, would anyone know how to adjust this? Thanks, Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ------------------------------ Message: 4 Date: Wed, 11 Aug 2010 12:46:46 +0000 (UTC) From: zodiac29@comcast.net Subject: [Histonet] Paraffin To: histonet@lists.utsouthwestern.edu Message-ID: <1464661975.1354742.1281530806687.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 Hello, Just wanted to know if anyone has tried Henry's Wonder Wax Xtreme Paraffin w/DMSO made by Mercedes Medical. What were your expirences with it? Is it comprable to McCormick's Paraplast Xtra? Thanks in advance, Jenny ------------------------------ Message: 5 Date: Wed, 11 Aug 2010 09:54:39 -0400 From: "Hannen, Valerie" Subject: [Histonet] Slide label printers To: Message-ID: <5680DA93771F0C48954CC8D38425E72401AB3512@ISMAIL.parrishmed.local> Content-Type: text/plain; charset="us-ascii" Hi gang... I am hoping that someone out there can lend me some assistance. We currently are using paper slide labels( 0.875 in by 0.875 in) on our Pathology slides, an old label printer (Genicom Model 1025) and Meditech Magic software. Our dilemma is this... we no longer can find the ink ribbons for our printer. Is there anyone out there who is using the same system? In about 1 1/2 to 2 years, we are going to be changing to a new software (possibly McKesson), so we are not looking to really buy anything expensive at this time. We just really need to continue using the paper labels until we have the change over. Thanks for your help. Valerie Hannen, MLT (ASCP),HTL,SU(FL) Parrish Medical Center Titusville,Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 12 **************************************** From schaundrawalton <@t> yahoo.com Wed Aug 11 12:56:25 2010 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Wed Aug 11 12:56:29 2010 Subject: [Histonet] Teaching Opportunity Message-ID: <858177.41065.qm@web120603.mail.ne1.yahoo.com> Keiser University in Orlando Florida is seeking an adjunct faculty member to teach evening classes for their Histotechnology program.? Candidates must have an Associate's degree and HT certification.? If interested please contact: ? Schaundra Walton BS, HTL(ASCP) Histotechnology Program Director Keiser University 5600 Lake Underhill Rd. Orlando, FL 32807 407-273-5800 ? ? From mbplab <@t> yahoo.com Wed Aug 11 13:20:42 2010 From: mbplab <@t> yahoo.com (Mary Benoit) Date: Wed Aug 11 13:20:47 2010 Subject: [Histonet] inverted meniscus Message-ID: <398180.10663.qm@web43140.mail.sp1.yahoo.com> Today while perfroming an xylene purity check after recyling xylene on a CBG recycler, I noticed that the meniscus??between the DH2O and Xylene is inverted...that is convex instead of concave.? I have done this check many times( you add?exactly 15 mls of water to exactly 85 mls xylene, invert and allow to settle out and?observe the meniscus at the separation site?for changes in volume of water)?and have never seen it invert.? The glass cyclinder was clean , we repeated three times and still same results.? Anyone have a clue as to why?? CBG did not have an answer .? thanks Mary F Benoit MT(ASCP) The Pathology Laboratory Lake Charles, LA From flnails <@t> texaschildrens.org Wed Aug 11 13:36:06 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Aug 11 13:36:21 2010 Subject: [Histonet] Sakura Tape coverslipper In-Reply-To: References: Message-ID: Has anyone been experiencing a brown cornflaking artifact on their slides after being coverslipped. I was told by Sakura that it was do to the slides drying out when transferred from the stainer to the coverslipper. Which can't be correct because on the new sakura coverslipper there is a holding area which is filled with xylene so if the tissue dried out it would rehydrate before being coverslipped. ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From Jackie.O'Connor <@t> abbott.com Wed Aug 11 13:37:39 2010 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Aug 11 13:41:28 2010 Subject: [Histonet] inverted meniscus In-Reply-To: <398180.10663.qm@web43140.mail.sp1.yahoo.com> References: <398180.10663.qm@web43140.mail.sp1.yahoo.com> Message-ID: Maybe it twisted it's knee while out playing touch football. From: Mary Benoit To: histonet@lists.utsouthwestern.edu Date: 08/11/2010 01:21 PM Subject: [Histonet] inverted meniscus Sent by: histonet-bounces@lists.utsouthwestern.edu Today while perfroming an xylene purity check after recyling xylene on a CBG recycler, I noticed that the meniscus between the DH2O and Xylene is inverted...that is convex instead of concave. I have done this check many times( you add exactly 15 mls of water to exactly 85 mls xylene, invert and allow to settle out and observe the meniscus at the separation site for changes in volume of water) and have never seen it invert. The glass cyclinder was clean , we repeated three times and still same results. Anyone have a clue as to why? CBG did not have an answer . thanks Mary F Benoit MT(ASCP) The Pathology Laboratory Lake Charles, LA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed Aug 11 13:50:08 2010 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Aug 11 13:50:08 2010 Subject: [Histonet] Sakura Tape coverslipper In-Reply-To: References: Message-ID: OMG. This problem was ever present in our lab for years, until I figured out that ANY minute trace of moisture, water, eosin in the last absolute alcohol would interfere with the activation of the adhesive in the tape, and create this brown artefact. We religiously change our absolute alcohols on the autostainer so the last alcohol is perfectly clear of any hint of eosin, and the artefact has disappeared from our tape-coverslipped slides. The Sakura rep couldn't help me either, and told me they had NEVER seen this problem before. Love it. Keep your last 100% PRISTINE, and the artefact will be eliminated. I promise. Jackie O' From: "Nails, Felton" To: "'Gauch, Vicki'" , "histonet@lists.utsouthwestern.edu" Date: 08/11/2010 01:45 PM Subject: [Histonet] Sakura Tape coverslipper Sent by: histonet-bounces@lists.utsouthwestern.edu Has anyone been experiencing a brown cornflaking artifact on their slides after being coverslipped. I was told by Sakura that it was do to the slides drying out when transferred from the stainer to the coverslipper. Which can't be correct because on the new sakura coverslipper there is a holding area which is filled with xylene so if the tissue dried out it would rehydrate before being coverslipped. ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmcgough <@t> clinlab.com Wed Aug 11 13:52:44 2010 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Wed Aug 11 13:52:52 2010 Subject: [Histonet] Sakura Tape coverslipper In-Reply-To: Message-ID: We had the same problem a few years ago and it was water in our xylene stations on the stainer. We change our xylene out more frequently now and the problem has not come up since. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Nails, Felton Sent: Wednesday, August 11, 2010 12:36 PM To: 'Gauch, Vicki'; histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura Tape coverslipper Has anyone been experiencing a brown cornflaking artifact on their slides after being coverslipped. I was told by Sakura that it was do to the slides drying out when transferred from the stainer to the coverslipper. Which can't be correct because on the new sakura coverslipper there is a holding area which is filled with xylene so if the tissue dried out it would rehydrate before being coverslipped. ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters <@t> uiowa.edu Wed Aug 11 14:07:43 2010 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Wed Aug 11 14:07:50 2010 Subject: [Histonet] purple spots Message-ID: Hi All, I recently did an Oil-Red-O stain using my usual procedure with formaldehyde vapor fixation, Harris hematoxylin, and finally Oil-Red-O. I was staining some frozen diseased aortic mouse tissue. This time the stain resulted in some dense purple spots in the tissue. I had not seen this before. I have posted pictures on the www.histonet.org website. The files are named low mag 2c, 2b, 2d, and 2e. The last three are a higher magnification of the first picture. I was hoping someone can tell me what these spots are. I suspect that they are simply necrotic tissue, but I would like the opinion of experts. Thanks for taking a look! Kathy Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walters@uiowa.edu www.uiowa.edu/~cemrf From quakermed <@t> gmail.com Wed Aug 11 14:58:32 2010 From: quakermed <@t> gmail.com (Olivia Lambe) Date: Wed Aug 11 14:58:35 2010 Subject: [Histonet] Leica ST5010/ST5020 vs Ventana Symphony Message-ID: For those of you who currently use or have used any of these instruments: Why did you chose it and how do you like? If you've used both, which do you like better and why or why not? We're currently manually testing. We're considering moving our lab into this century and need a little advice. Thank you! From Vickroy.Jim <@t> mhsil.com Wed Aug 11 14:58:50 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Aug 11 14:58:54 2010 Subject: [Histonet] fragmentation on core bone marrow biopsies Message-ID: <24A4826E8EF0964D86BC5317306F58A554D2234DDF@mmc-mail.ad.mhsil.com> We are looking at the cause of fragmentation on our bone marrow core biopsies. We are reviewing fixative times and decalcification times. Has anybody else experienced fragmentation problems? If so how did you eliminate the problem? What other variables do I need to look at that might be the cause? Thanks for the suggestions in advance. We do use an AZF fixative and a gentle decal solution called Rapid-cal. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From mwfolsom <@t> rgbio.com Wed Aug 11 17:47:32 2010 From: mwfolsom <@t> rgbio.com (Michael Folsom) Date: Wed Aug 11 17:43:05 2010 Subject: [Histonet] Re: test animal vs. plant In-Reply-To: <1279793945.4143.1386127399@webmail.messagingengine.com> References: <1279793945.4143.1386127399@webmail.messagingengine.com> Message-ID: <1281566852.7065.15.camel@chico.322tulane.org> With phloroglucinol you are staining for lignins which can be present in varying abouts in plant tissue. Also, please note that different types of lignins occur in different types of plants at different levels so sadly negative results don't mean much. Other carbos to consider are cellulose or callose - calcoflour white is an easy to use stain (0.1% in dH2O) for cellulose and flouresces in the UV range, aniline blue stains callose also in the UV range. I know none of these are diagnostic by themselves but if you tie it to some macerates and see fibers or trachieds then you have some plant tissue. Mike Rio Grande Biological Albuquerque, NM On Thu, 2010-07-22 at 20:19 +1000, Birgitta Stephenson wrote: > Hello Tamara, > > Thanks for your reply. In the lab we have worked with the carbohydrate > ideas, so travelling down the same path. With the grindstones we are > looking for some tests that we can also carry out in the field as > sometimes we have grinding hollows or stones in situ which can not be > moved. This is why we thought simple visual staining and utilising a > hand held digital microscope could be the answer. However the staining > is not as straight forward as we'd hoped. I am thinking that if the > Phloroglucinol is light sensitive then perhaps counterstaining with > Toluidine Blue can highlight other areas. Problem is the residues are > water lifted and drowning the slide with tap water (well drops of) means > that the residue at times almost vanishes. Any more thoughts are most > welcome. > > Thnaks again > > Birgitta Stephenson > > Research Microscopy Lab, University of Queensland > On Tue, 20 Jul 2010 11:41:51 -0600, "Tamara A Howard" > said: > > I saw your post on the Histonet - it seems that there must > > specific tests that could do what you need. I Googled the > > word string: "test distinguish plant animal" & got the > > following promising hit: > > > > http://www.hsc.csu.edu.au/chemistry/options/forensic/2964/ch992nov03.html > > > > My guess is that there would be more info from forensic > > science sites; they must have listservers, too. > > > > It sounds like a very interesting project - I'm envious! > > > > Tamara > > > > *************************** > > Tamara Howard > > Cell Biology & Physiology > > UNM-HSC > > Albuquerque, NM > > *************************** > -- > Birgitta Stephenson > bstephen@fastmail.fm > From aazath <@t> hotmail.com Wed Aug 11 19:40:26 2010 From: aazath <@t> hotmail.com (Aazath Raj) Date: Wed Aug 11 19:40:29 2010 Subject: [Histonet] PAP In-Reply-To: References: Message-ID: Dear friends, does anyone have rapid PAP(cytostain) preparation,for rapid PAP staining. Aazathraj.P Technical Officer Department of Histopathology and Cytology Apollo Hospitals chennai India From aazath <@t> hotmail.com Wed Aug 11 19:47:57 2010 From: aazath <@t> hotmail.com (Aazath Raj) Date: Wed Aug 11 19:48:00 2010 Subject: [Histonet] RE: Histonet Digest, Vol 80, Issue 31 In-Reply-To: References: Message-ID: Dear Friends, Does anyone have rehydration technique of air dried smear which can be stained for pap. Aazathraj.P Technical Officer Department of Histopathology and Cytology Apollo Hospitals -chennai India From kjgada <@t> gmail.com Thu Aug 12 05:02:00 2010 From: kjgada <@t> gmail.com (Komal Gada) Date: Thu Aug 12 05:02:03 2010 Subject: [Histonet] Question about Control for Ramnowski Stain Message-ID: Hello Histonetters, I'm looking for what the best paraffin control to use for a Ramnowski stain to show differentiation in the tissue (not blood or bone marrow)? Thanks as always for your help! Komal From lelmgren <@t> sunriselab.com Thu Aug 12 07:15:45 2010 From: lelmgren <@t> sunriselab.com (Laurie Elmgren) Date: Thu Aug 12 07:15:50 2010 Subject: [Histonet] Nail Problems Message-ID: <4A672C6AE0402D4A89ECE29E8A4B47E3029E98CD@MailPDC.sunriselab.com> Does anyone have a sure fire way of keeping nails attached to the slide? We use Sta-On and charged slides, have used albumin, dried overnight, and still have difficulty keeping them attached during staining. Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515x1108 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." From jaylundgren <@t> gmail.com Thu Aug 12 08:33:39 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Aug 12 08:33:47 2010 Subject: [Histonet] inverted meniscus In-Reply-To: References: <398180.10663.qm@web43140.mail.sp1.yahoo.com> Message-ID: LOL Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Cline <@t> wchsys.org Thu Aug 12 08:59:42 2010 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Thu Aug 12 09:14:39 2010 Subject: [Histonet] Leica ST5010/ST5020 vs Ventana Symphony In-Reply-To: References: Message-ID: We use the Lecia ST5020 for our paps and it performs just fine. We looked into a Symphony but reagent cost was too high for us compared to what we pay now. ________________________________________ For those of you who currently use or have used any of these instruments: Why did you chose it and how do you like? If you've used both, which do you like better and why or why not? We're currently manually testing. We're considering moving our lab into this century and need a little advice. Thank you! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From PMonfils <@t> Lifespan.org Thu Aug 12 09:39:56 2010 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Aug 12 09:40:00 2010 Subject: [Histonet] Slide expiry In-Reply-To: References: Message-ID: <4EBFF65383B74D49995298C4976D1D5E0707EEAA@LSRIEXCH1.lsmaster.lifespan.org> Don't think of it as "the date the slides stop working". It's just the date the company has set for the termination of their liability. If there was a problem with the slides prior to that date, the company would replace them. After that date, they don't have to. But most likely the slides will be good well beyond that date, assuming they were stored properly. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thotakura, Anil Kumar Sent: Tuesday, August 10, 2010 10:16 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide expiry Dear All, I purchased bulk of Slides polysin coated slides and on the box it say they expire by end of this year, Does it really effect ?? Or I don't have to bother. Please let me know. Many Thanks, Anil Kumar. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Thu Aug 12 10:51:37 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Aug 12 10:51:41 2010 Subject: [Histonet] metal base molds for paraffin embedding Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24554@PHSXMB30.partners.org> To all: We need to order metal base molds for paraffin embedding. We need a size @45mm(L) X 32mm(W) X 11mm(D). (We have some, but have no idea where and when we ordered them). I have looked in several catalogs but can only find a depth of 5mm. The depth is the important part. If someone knows where I can order these, I would appreciate it. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From KMB01 <@t> grh.org Thu Aug 12 10:55:44 2010 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Thu Aug 12 10:55:49 2010 Subject: [Histonet] CAP question Message-ID: This will be my first CAP inspection. We are switching from JCAHO. Somewhere I read that we need to have a grossing room manual that would have all the procedures on how to gross anything and everything that would come through the Histology Lab. Is this true? Does anyone have a sample that they are willing to share with me? Thanks, Kathy Gorham H.T. Grande Ronde Hospital La Grande, OR GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From trathborne <@t> somerset-healthcare.com Thu Aug 12 11:03:09 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Aug 12 11:03:16 2010 Subject: [Histonet] metal base molds for paraffin embedding In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24554@PHSXMB30.partners.org> Message-ID: Have you tried Surgipath? I think that's where we got ours. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sherwood, Margaret Sent: Thursday, August 12, 2010 11:52 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] metal base molds for paraffin embedding To all: We need to order metal base molds for paraffin embedding. We need a size @45mm(L) X 32mm(W) X 11mm(D). (We have some, but have no idea where and when we ordered them). I have looked in several catalogs but can only find a depth of 5mm. The depth is the important part. If someone knows where I can order these, I would appreciate it. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Bill.Tench <@t> pph.org Thu Aug 12 11:04:26 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Thu Aug 12 11:04:32 2010 Subject: [Histonet] grossing manual Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5527@MAIL1.pph.local> Yes, you do need a "standard procedure" manual for the management of all of your specimens. Life has gotten more complicated with the reporting standards now in place for reporting cancer which includes the gross examination. A good start for the non-neoplastic cases would be the appendix of a good surgical pathology text like Rosai (i haven't seen the latest editions, but i think it is still there). Other standard references also have this information. And then, go to the CAP website and download the standard protocols and convert them to how your pathologists want them. All of this may be online, but you also need a copy in the gross room. It's a pretty sizable job if you don't have any of it done already. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- From lori.garcia <@t> medtronic.com Thu Aug 12 11:12:19 2010 From: lori.garcia <@t> medtronic.com (Garcia, Lori, Sr. Scientist) Date: Thu Aug 12 11:12:31 2010 Subject: [Histonet] metal base molds for paraffin embedding In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24554@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24554@PHSXMB30.partners.org> Message-ID: <5A8A2A45BE610D459A95CD6A9102102A0106DBA522@STSM1BMSGM04.ent.core.medtronic.com> Hi Peggy, We ordered these base molds from vwr.com TISSUE TEK MEGA BASE MOLD, 6/PK (4166 Catalog Number: 100490-190 Supplier: Electron Microscopy Sciences Description: TISSUE TEK MEGA BASE MOLD, 6/PK (4166). stainless steel, reusable base mold is available, measures 31mm(L)x23mm(W)x13.5mm(D) Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Thursday, August 12, 2010 8:52 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] metal base molds for paraffin embedding To all: We need to order metal base molds for paraffin embedding. We need a size @45mm(L) X 32mm(W) X 11mm(D). (We have some, but have no idea where and when we ordered them). I have looked in several catalogs but can only find a depth of 5mm. The depth is the important part. If someone knows where I can order these, I would appreciate it. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From sbreeden <@t> nmda.nmsu.edu Thu Aug 12 11:20:00 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Aug 12 11:20:06 2010 Subject: [Histonet] Metal Base Molds Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E472E4@nmdamailsvr.nmda.ad.nmsu.edu> Try Electron Microscopy Sciences ( www.emsdiasum.com). They are a treasure-trove of goodies like base molds. They have things in their catalog you didn't know you needed until you see it! I just looked and they have many sizes of base molds. Shop! Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 From integrated.histo <@t> gmail.com Thu Aug 12 11:32:15 2010 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Thu Aug 12 11:32:21 2010 Subject: [Histonet] Used Lab Equipment Message-ID: What is the best way to dispose of used lab equipment. All pieces are in working condition, but we are closing our doors. Cindy DuBois Integrated Pathology From chapcl <@t> yahoo.com Thu Aug 12 11:54:28 2010 From: chapcl <@t> yahoo.com (William Chappell) Date: Thu Aug 12 11:54:32 2010 Subject: [Histonet] Biogenex Message-ID: <10170.14156.qm@web30603.mail.mud.yahoo.com> I heard a rumor that Biogenex was recently purchased. Can anyone confirm or deny said rumor? Will From christiegowan <@t> msn.com Thu Aug 12 12:16:09 2010 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Aug 12 12:16:13 2010 Subject: [Histonet] Leica ST5010/ST5020 vs Ventana Symphony In-Reply-To: References: Message-ID: Hi Olivia, We currently use the Symphony stainer and based on our lab's needs, we love it. When choosing a stainer, you need to look at what your needs are. We stained so many racks of slides a day on our old Leica Autostainer that we were constantly changing solutions. Also, if we put more than 4 racks at a time on to stain, it bogged down the whole process. We used a separate oven to dry in so we were getting up to load the oven, getting up to load the stainer, getting up to change solutions and so we were making a lot of steps for one process. The Symphony is an enclosed system that contains 3 stainers, 2 ovens, one coverslipper and a bar code reader. When you purchase the system you are locked into their reagents so you need to see if this is cost effective for your lab. We were able to eliminate one position after purchasing the Symphony as well as our need to pay for waste disposal. I will say that we also use the vantage system in our lab and that has also impacted our eliminating the one position. We still use our Leica stainer and coverslipper for research and education and for a backup if the Symphony is down. They are both good stainers and each have their pros and cons. The other issue to think about is size and real estate. The Leica's are benchtop stainers and the Symphony is a stand alone which needs to be vented as well as distilled water flow. Hope this helps you a little. Christie Gowan UAB Medical Center Birmingham, AL > Date: Wed, 11 Aug 2010 12:58:32 -0700 > From: quakermed@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica ST5010/ST5020 vs Ventana Symphony > > For those of you who currently use or have used any of these instruments: > Why did you chose it and how do you like? If you've used both, which do you > like better and why or why not? > > We're currently manually testing. We're considering moving our lab into this > century and need a little advice. > > Thank you! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Thu Aug 12 12:42:41 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Aug 12 12:42:48 2010 Subject: [Histonet] metal base molds Message-ID: The following link has larger molds, maybe one of them will work. http://www.brainresearchlab.com/productinform.html Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 Message: 20 Date: Thu, 12 Aug 2010 11:51:37 -0400 From: "Sherwood, Margaret " Subject: Re: [Histonet] metal base molds for paraffin embedding To: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24554@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" To all: We need to order metal base molds for paraffin embedding. We need a size @45mm(L) X 32mm(W) X 11mm(D). (We have some, but have no idea where and when we ordered them). I have looked in several catalogs but can only find a depth of 5mm. The depth is the important part. If someone knows where I can order these, I would appreciate it. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ From ROrr <@t> northshore.org Thu Aug 12 12:45:17 2010 From: ROrr <@t> northshore.org (Orr, Rebecca) Date: Thu Aug 12 12:45:21 2010 Subject: [Histonet] Ventana ES labels In-Reply-To: <993d8278-1004-41bd-9f42-5d1a72cc0a97@EXCHCAS01.enhnet.org> Message-ID: Message: 1 Date: Wed, 11 Aug 2010 13:05:15 -0400 From: "Gauch, Vicki" Subject: [Histonet] Ventana ES labels To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, I am posting this for a colleague of mine....She is in the middle of a project and found out that she can no longer order Ventana ES labels. She wants to know if anyone got rid of their ES stainer but still has some labels in their lab that they are looking to get rid of....or if anyone knows where she might find some labels... Any help would be greatly appreciated. Please respond to my e-mail and I will get the information to her since she is not on the Histonet... Thanks, Vicki Gauch AMCH Albany, NY ............................................................. Vicki, I'm in the middle of Feng Shui-ing my lab and found a sack full of ES labels just this morning! That's how the Histo- universe rotates, what one needs to toss out, another needs to have. Please give her my email address so we can move these labels into their true destiny. Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 From cbrya <@t> lexclin.com Thu Aug 12 14:01:41 2010 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Aug 12 14:01:47 2010 Subject: [Histonet] voice recognition Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF05B0BBC967@EXCHANGESB> Is anyone using voice recognition with SoftPath for dictating? If so, what software program are you using and does it work well? Thank you in advance for any comments. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From MSHERWOOD <@t> PARTNERS.ORG Thu Aug 12 14:22:17 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Aug 12 14:22:27 2010 Subject: [Histonet] Used Lab Equipment In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24556@PHSXMB30.partners.org> I would contact the many companies who deal with refurbished equipment (we bought a stainer and coverslipper via this route. Saved a lot of money) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Thursday, August 12, 2010 12:32 PM To: Histonet Subject: [Histonet] Used Lab Equipment What is the best way to dispose of used lab equipment. All pieces are in working condition, but we are closing our doors. Cindy DuBois Integrated Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From drvet_anjan <@t> hotmail.com Thu Aug 12 14:59:00 2010 From: drvet_anjan <@t> hotmail.com (Anjan Kumar) Date: Thu Aug 12 14:59:04 2010 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <591844906.7853.1281643140768.JavaMail.app@ech3-cdn06.prod> LinkedIn ------------Anjan Kumar requested to add you as a connection on LinkedIn: ------------------------------------------ Jackie, I'd like to add you to my professional network on LinkedIn. - Anjan Accept invitation from Anjan Kumar http://www.linkedin.com/e/yvpgd1-gcs18gik-3t/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I801858559_3/pmpxnSRJrSdvj4R5fnhv9ClRsDgZp6lQs6lzoQ5AomZIpn8_cRYVdjkUdjwNc3x9bTtTm6xjrP5xbP4Pe3sVc3wRdj4LrCBxbOYWrSlI/EML_comm_afe/ View invitation from Anjan Kumar http://www.linkedin.com/e/yvpgd1-gcs18gik-3t/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I801858559_3/0PnPARdjwRe34Me4ALqnpPbOYWrSlI/svi/ ------------------------------------------ Why might connecting with Anjan Kumar be a good idea? People Anjan Kumar knows can discover your profile: Connecting to Anjan Kumar will attract the attention of LinkedIn users. See who's been viewing your profile: http://www.linkedin.com/e/yvpgd1-gcs18gik-3t/wvp/inv18_wvmp/ ------ (c) 2010, LinkedIn Corporation From Kimberly.Poole <@t> drdc-rddc.gc.ca Thu Aug 12 15:02:12 2010 From: Kimberly.Poole <@t> drdc-rddc.gc.ca (Poole, Kimberly) Date: Thu Aug 12 15:02:22 2010 Subject: [Histonet] Re: Blade Angle on Microtome Message-ID: <42DFE1A029181B4B8CCBA7261B52D76501454EE8@suffieldex01.suffield.drdc-rddc.gc.ca> Hi everyone, I have a really simple question to ask. What angle would you have your blade at on your microtome machine? Right now mine is set to 0 but I am wondering if I should increase this because I am seeing some vibration. Thanks for your help! Kimberly Kimberly Poole B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.poole@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada From talulahgosh <@t> gmail.com Thu Aug 12 15:05:24 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Aug 12 15:05:29 2010 Subject: [Histonet] Invitation to connect on LinkedIn In-Reply-To: <591844906.7853.1281643140768.JavaMail.app@ech3-cdn06.prod> References: <591844906.7853.1281643140768.JavaMail.app@ech3-cdn06.prod> Message-ID: to the moderator: this is spam, which we will get repeatedly. emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Thu, Aug 12, 2010 at 3:59 PM, Anjan Kumar wrote: > LinkedIn > ------------Anjan Kumar requested to add you as a connection on LinkedIn: > ------------------------------------------ > > Jackie, > > I'd like to add you to my professional network on LinkedIn. > > - Anjan > > Accept invitation from Anjan Kumar > > http://www.linkedin.com/e/yvpgd1-gcs18gik-3t/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I801858559_3/pmpxnSRJrSdvj4R5fnhv9ClRsDgZp6lQs6lzoQ5AomZIpn8_cRYVdjkUdjwNc3x9bTtTm6xjrP5xbP4Pe3sVc3wRdj4LrCBxbOYWrSlI/EML_comm_afe/ > > View invitation from Anjan Kumar > > http://www.linkedin.com/e/yvpgd1-gcs18gik-3t/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I801858559_3/0PnPARdjwRe34Me4ALqnpPbOYWrSlI/svi/ > > ------------------------------------------ > > Why might connecting with Anjan Kumar be a good idea? > > People Anjan Kumar knows can discover your profile: > Connecting to Anjan Kumar will attract the attention of LinkedIn users. See > who's been viewing your profile: > > http://www.linkedin.com/e/yvpgd1-gcs18gik-3t/wvp/inv18_wvmp/ > > > ------ > (c) 2010, LinkedIn Corporation > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mucram11 <@t> comcast.net Thu Aug 12 15:06:23 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Thu Aug 12 15:06:27 2010 Subject: [Histonet] voice recognition In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF05B0BBC967@EXCHANGESB> Message-ID: <2023127769.108106.1281643583542.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Yes, we currently use ProVox and are thinking of changing.? It still requires editing before being completed in Soft.? There are issues and they can be difficult.? Let me know if you want to talk about it and I can call you tomorrow. Pam Marcum UAMS Little Rock, AR ----- Original Message ----- From: "Carol Bryant" To: "Histonet@lists.utsouthwestern.edu" Sent: Thursday, August 12, 2010 2:01:41 PM Subject: [Histonet] voice recognition Is anyone using voice recognition with SoftPath for dictating? ?If so, what software program are you using and does it work well? Thank you in advance for any comments. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. ?If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. ?Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet UAMS Little Rock, AR ----- Original Message ----- From: "Carol Bryant" To: "Histonet@lists.utsouthwestern.edu" Sent: Thursday, August 12, 2010 2:01:41 PM Subject: [Histonet] voice recognition Is anyone using voice recognition with SoftPath for dictating? ?If so, what software program are you using and does it work well? Thank you in advance for any comments. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. ?If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. ?Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Aug 12 15:06:40 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Aug 12 15:06:45 2010 Subject: [Histonet] Re: Blade Angle on Microtome In-Reply-To: <42DFE1A029181B4B8CCBA7261B52D76501454EE8@suffieldex01.suffield.drdc-rddc.gc.ca> References: <42DFE1A029181B4B8CCBA7261B52D76501454EE8@suffieldex01.suffield.drdc-rddc.gc.ca> Message-ID: We always section at 4 degrees, paraffin and frozen. This is for fixed chick or mouse embryo tissue. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Thu, Aug 12, 2010 at 4:02 PM, Poole, Kimberly < Kimberly.Poole@drdc-rddc.gc.ca> wrote: > Hi everyone, > > > > I have a really simple question to ask. What angle would you have your > blade at on your microtome machine? Right now mine is set to 0 but I am > wondering if I should increase this because I am seeing some vibration. > Thanks for your help! > > > > Kimberly > > > > Kimberly Poole B.Sc > > Casualty Management Section | Section de la gestion des bless?s > > Defence Research and Development Canada Suffield | Recherche et > d?veloppement pour la d?fense Canada Suffield > > Medicine Hat, AB, Canada T1A 8K6 > > kimberly.poole@drdc-rddc.gc.ca > > Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 > > Government of Canada | Gouvernement du Canada > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Kimberly.Poole <@t> drdc-rddc.gc.ca Thu Aug 12 15:12:10 2010 From: Kimberly.Poole <@t> drdc-rddc.gc.ca (Poole, Kimberly) Date: Thu Aug 12 15:12:16 2010 Subject: [Histonet] (no subject) Message-ID: <42DFE1A029181B4B8CCBA7261B52D76501454EEB@suffieldex01.suffield.drdc-rddc.gc.ca> Hi everyone! I have a really simple question to ask. What angle would you have your blade at on your microtome machine? Right now mine is set to 0 but I am wondering if I should increase this because I am seeing some vibration. I am cutting rat and swine brain tissue. Thanks for your help! Kimberly Poole B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.poole@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada From Kimberly.Poole <@t> drdc-rddc.gc.ca Thu Aug 12 15:12:49 2010 From: Kimberly.Poole <@t> drdc-rddc.gc.ca (Poole, Kimberly) Date: Thu Aug 12 15:12:53 2010 Subject: [Histonet] Re: Angle of Blade on Microtome Message-ID: <42DFE1A029181B4B8CCBA7261B52D76501454EED@suffieldex01.suffield.drdc-rddc.gc.ca> Hi everyone! I have a really simple question to ask. What angle would you have your blade at on your microtome machine? Right now mine is set to 0 but I am wondering if I should increase this because I am seeing some vibration. I am cutting rat and swine brain tissue. Thanks for your help! Kimberly Poole B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.poole@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada From sgoebel <@t> xbiotech.com Thu Aug 12 15:27:46 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Thu Aug 12 15:27:56 2010 Subject: [Histonet] Used Lab Equipment Message-ID: <20100812132746.9e2d9aa830e8449a2412eb1e4f2f067e.a4583a913d.wbe@email04.secureserver.net> What equipment do you have? We might be interested in purch Sarah Goebel, B.A., HT (ASCP) < XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] Used Lab Equipment From: "Sherwood, Margaret " <[1]M Date: Thu, August 12, 2010 12:22 pm To: "Cindy DuBois" <[2]integr <[3]histonet@lists.uts I would contact the many companies who deal with refurbished equipment (we< lot of money) -----Original Message----- From: [4]histonet [[5]mailto:histon Cindy DuBois Sent: Thursday, August 12, 2010 12:32 PM To: Histonet Subject: [Histonet] Used Lab Equipment What is the best way to dispose of used lab equipment. All pieces are in working condition, but we are closing our doors. Cindy DuBois Integrated Pathology _______________________________________________ Histonet mailing list [6]Histonet@lists.utsouth [7]http: The information in this e-mail is intended only for the person to whom it i addressed. If you believe this e-mail was sent to you in error and the e-ma contains patient information, please contact the Partners Compliance HelpLi [8]http://www.partners.org/ to you in error but does not contain patient information, please contact the sender and pro dispose of the e-mail. _______________________________________________ Histonet mailing list [9]Histonet@lists.utsouth [10]http: References 1. 3D"mailto:MSHERWOOD@PARTNERS.ORG" 2. 3D"mailto:integrated.histo@gmail.com" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:Histonet@lists.utsouthwestern.edu" 7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 8. 3D"http://www.partners.org/complianceline" 9. 3D"mailto:Histonet@lists.utsouthwestern.edu" 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From sgoebel <@t> xbiotech.com Thu Aug 12 15:29:03 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Thu Aug 12 15:29:09 2010 Subject: [Histonet] Re: Angle of Blade on Microtome Message-ID: <20100812132903.9e2d9aa830e8449a2412eb1e4f2f067e.b4f2f5c764.wbe@email04.secureserver.net> Usually I set mine around 5. You just have to play with you microtome to get the right setting Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 E Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] Re: Angle of Blade on Microtome From: "Poole, Kimberly" <[1]Kimberly.Poole@drdc-rddc.gc.ca> Date: Thu, August 12, 2010 1:12 pm To: <[2]histonet@lists Hi everyone! I have a really simple question to ask. What angle would you have your blad am wonderi vibration. I am cutti help! Kimberly Poole [3]B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?velo Medicine Hat, AB, Canada T1A 8K6 [4]kimberly.poole@drdc-rddc. erly.poole@drdc-rddc.gc.ca> Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?co Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list [6]Histonet@lists.utsouth [7]http: References 1. 3D"mailto:Kimberly.Poole@drdc-rddc.gc.c 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"http://B.Sc"/ 4. 3D"mailto:kimberly.poole@drdc-rddc.gc.ca" 5. 3D"mailto:kimberly.poole@drdc-rddc.gc.ca" 6. 3D"mailto:Histonet@lists.utsouthwestern.edu" 7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From abright <@t> brightinstruments.com Thu Aug 12 15:42:18 2010 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Aug 12 15:45:02 2010 Subject: [Histonet] Posting on Histonet Message-ID: <1850021592-1281645825-cardhu_decombobulator_blackberry.rim.net-1258871036-@bda040.bisx.produk.on.blackberry> I am not able to post on Histonet with a blackberry, have asked before but never been informed why all of a sudden its a problem. There are many times I could help with a posting. Hope you can sort this out, thanks Alan Bright , Bright Instruments. England Sent from my BlackBerry? wireless device From elciba <@t> hotmail.com Thu Aug 12 16:06:03 2010 From: elciba <@t> hotmail.com (ricky hachy) Date: Thu Aug 12 16:06:09 2010 Subject: [Histonet] Used Lab Equipment In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24556@PHSXMB30.partners.org> References: , <073AE2BEA1C2BA4A8837AB6C4B943D9703E24556@PHSXMB30.partners.org> Message-ID: What equipments do you have ? Maybe I could buy some . Ricky > Date: Thu, 12 Aug 2010 15:22:17 -0400 > From: MSHERWOOD@PARTNERS.ORG > To: integrated.histo@gmail.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Used Lab Equipment > CC: > > I would contact the many companies who deal with refurbished equipment (we > bought a stainer and coverslipper via this route. Saved a lot of money) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois > Sent: Thursday, August 12, 2010 12:32 PM > To: Histonet > Subject: [Histonet] Used Lab Equipment > > What is the best way to dispose of used lab equipment. All pieces are > in working condition, but we are closing our doors. > > Cindy DuBois > Integrated Pathology > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ross <@t> premierlab.com Thu Aug 12 16:52:13 2010 From: ross <@t> premierlab.com (Ross Benik) Date: Thu Aug 12 16:51:18 2010 Subject: [Histonet] Pauly reagent Message-ID: Does anyone by any chance have a standard protocol for histidine staining using Pauly Reagent? Thanks in advance -Ross From Traczyk7 <@t> aol.com Thu Aug 12 21:22:25 2010 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Thu Aug 12 21:22:45 2010 Subject: [Histonet] blade angle Message-ID: <4b35.1acde149.39960661@aol.com> Dear Kim, It is very likely that the markings on your knife block are reference points, not actual degree markings. If "0" has worked for you all along, then I would think that the vibrations are caused by something other than knife angle. If you are using disposable blades, there might be a variation in how high the edge of the blade sits above the top of the holder. If you are just starting out and have never really been happy with the quality of your cut sections then changing the angle could help. Since you are already at zero, make a slight change and see if that helps. Just remember, when you change the knife angle you change the orientation of block face to knife edge. Be careful not to damage your block and/or knife. If you have any questions, feel free to contact me off list. Regards, Dorothy Dorothy Traczyk MTA Histology LLC PO Box 602 Point Pleasant, NJ 08742 T: 732-899-2912 F: 732-899-5469 _dorothy@mtahistology.com_ (mailto:dorothy@mtahistology.com) From G.Spoelstra <@t> murdoch.edu.au Fri Aug 13 04:55:51 2010 From: G.Spoelstra <@t> murdoch.edu.au (Gerard Spoelstra) Date: Fri Aug 13 04:56:05 2010 Subject: [Histonet] Nail Problems References: <4A672C6AE0402D4A89ECE29E8A4B47E3029E98CD@MailPDC.sunriselab.com> Message-ID: Hi Laurie, I have had success keeping the nails on using gelatin coated slides. I make up the gelatin in beaker and dissolve it using the microwave. Then coat the slide and pick the section from the water bath using the the just coated slide. I then dry the slide overnight in the 37degree oven. You may need to vary the concentration of the gelatin. My first attempts were successful because the gelatin wasn't concentrated enough. The reason I think that the nail won't stick is because you can't get it to sit flat. Also you can first float the section on cold water. I can't remember whether I picked up the section only from cold water exclusively. I think that nails have to be one of the most difficult tissues to keep on a slide. Gerard Spoelstra Veterinary Histology Murdoch University Western Australia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Elmgren Sent: Thu 8/12/2010 8:15 PM To: Histonet Subject: [Histonet] Nail Problems Does anyone have a sure fire way of keeping nails attached to the slide? We use Sta-On and charged slides, have used albumin, dried overnight, and still have difficulty keeping them attached during staining. Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515x1108 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Philip.Gibson <@t> nuth.nhs.uk Fri Aug 13 07:23:34 2010 From: Philip.Gibson <@t> nuth.nhs.uk (Gibson, Philip) Date: Fri Aug 13 07:23:46 2010 Subject: [Histonet] Acceptable way to "bake" sections onto slides? Message-ID: <1966D567F43F8A4AA975C2B46F83088307410E@nuth-rex01.xnuth.nhs.uk> Hi In our "fairly large" histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to "bake" for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil ---------------------------------------- Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net From annigyg <@t> gmail.com Fri Aug 13 07:31:11 2010 From: annigyg <@t> gmail.com (annigyg@gmail.com) Date: Fri Aug 13 07:31:00 2010 Subject: [Histonet] Acceptable way to "bake" sections onto slides? In-Reply-To: <1966D567F43F8A4AA975C2B46F83088307410E@nuth-rex01.xnuth.nhs.uk> References: <1966D567F43F8A4AA975C2B46F83088307410E@nuth-rex01.xnuth.nhs.uk> Message-ID: <1925768825-1281702649-cardhu_decombobulator_blackberry.rim.net-880166395-@bda269.bisx.produk.on.blackberry> Use the sakura DRS hotpot Loading from the plate (apart for the other drawbacks) will set the wax on the first slides put in the racks and you may end uo with inadequate dewaxing Just my 5cents worth Annieinarabia Empower your Business with BlackBerry? and Mobile Solutions from Etisalat -----Original Message----- From: "Gibson, Philip" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Fri, 13 Aug 2010 13:23:34 To: Subject: [Histonet] Acceptable way to "bake" sections onto slides? Hi In our "fairly large" histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to "bake" for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil ---------------------------------------- Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 13 07:35:50 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 13 07:35:55 2010 Subject: [Histonet] Acceptable way to "bake" sections onto slides? In-Reply-To: <1966D567F43F8A4AA975C2B46F83088307410E@nuth-rex01.xnuth.nhs.uk> Message-ID: <797872.87743.qm@web65713.mail.ac4.yahoo.com> The correct answer is "b": if there is water underneath the section that is going to be placed on the hot plate, the most likely artifact is that of "empty nuclei" that will ruin the sections' usefulness. Ren? J. --- On Fri, 8/13/10, Gibson, Philip wrote: From: Gibson, Philip Subject: [Histonet] Acceptable way to "bake" sections onto slides? To: histonet@lists.utsouthwestern.edu Date: Friday, August 13, 2010, 8:23 AM Hi In our "fairly large" histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to "bake" for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil ---------------------------------------- Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Fri Aug 13 08:45:40 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Aug 13 08:56:17 2010 Subject: [Histonet] C4d on paraffin sections Message-ID: <61135F0455D33347B5AAE209B903A30433DEBC41@EXCHVS2.medctr.ad.wfubmc.edu> I need some advice. We have been trying to do C4d on paraffin sections without much success. Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much! Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 From tpodawiltz <@t> lrgh.org Fri Aug 13 09:01:16 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Aug 13 09:01:24 2010 Subject: [Histonet] Acceptable way to "bake" sections onto slides? In-Reply-To: <797872.87743.qm@web65713.mail.ac4.yahoo.com> References: <1966D567F43F8A4AA975C2B46F83088307410E@nuth-rex01.xnuth.nhs.uk> <797872.87743.qm@web65713.mail.ac4.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5DF8366@LRGHEXVS1.practice.lrgh.org> Totally agree with Rene. You will need to use some type of drying oven Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 13, 2010 8:36 AM To: histonet@lists.utsouthwestern.edu; PhilipGibson Subject: Re: [Histonet] Acceptable way to "bake" sections onto slides? The correct answer is "b": if there is water underneath the section that is going to be placed on the hot plate, the most likely artifact is that of "empty nuclei" that will ruin the sections' usefulness. Ren? J. --- On Fri, 8/13/10, Gibson, Philip wrote: From: Gibson, Philip Subject: [Histonet] Acceptable way to "bake" sections onto slides? To: histonet@lists.utsouthwestern.edu Date: Friday, August 13, 2010, 8:23 AM Hi In our "fairly large" histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to "bake" for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil ---------------------------------------- Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From tdobersztyn <@t> chmca.org Fri Aug 13 09:10:12 2010 From: tdobersztyn <@t> chmca.org (Dobersztyn, Theresa) Date: Fri Aug 13 09:11:46 2010 Subject: [Histonet] (no subject) Message-ID: Hello all! We are looking to purchase a coverslipper. would love opinions on what you are using. Thanks in advance! Theresa Dobersztyn HT (ASCP) Akron Children's Hospital From matt <@t> techoneweb.com Fri Aug 13 09:19:54 2010 From: matt <@t> techoneweb.com (matt@techoneweb.com) Date: Fri Aug 13 09:19:57 2010 Subject: [Histonet] Re: Blade Angle on Microtome Message-ID: <6a51c513cc62e47989e4c464e0b3c236.squirrel@www.techoneweb.com> Key Kim, The blade angle depends on the manufacturer of the microtome. In general, Leica suggests and angle of 3 to 5. Microm uses 10 to 12 and Shandon uses 0 to 3. Confused? Me too and I repair them for a living. I have found that vibration is caused by problems with the knife holder more often than knife angle. The problem can be as simple as the pressure plate not being tight enough. Let me know what type of tome you have and I may me able to help. Best Matt Mincer Tech One Biomedical 708-822-3738 From Ronald.Houston <@t> nationwidechildrens.org Fri Aug 13 09:29:11 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Aug 13 09:29:17 2010 Subject: [Histonet] RE: C4d on paraffin sections In-Reply-To: <61135F0455D33347B5AAE209B903A30433DEBC41@EXCHVS2.medctr.ad.wfubmc.edu> References: <61135F0455D33347B5AAE209B903A30433DEBC41@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: We use the C4d from Cell Marque now with great results; it is an IVD. ER1 20 minutes on the BondMax and Bond III Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Friday, August 13, 2010 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d on paraffin sections I need some advice. We have been trying to do C4d on paraffin sections without much success. Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much! Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Aug 13 09:44:00 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Aug 13 09:44:28 2010 Subject: [Histonet] RE: C4d on paraffin sections In-Reply-To: References: <61135F0455D33347B5AAE209B903A30433DEBC41@EXCHVS2.medctr.ad.wfubmc.edu>, Message-ID: We use the same antibody from Cell Marque on the Dako Autostainers with great success. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Sent: Friday, August 13, 2010 10:29 AM To: 'Martha Ward'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: C4d on paraffin sections We use the C4d from Cell Marque now with great results; it is an IVD. ER1 20 minutes on the BondMax and Bond III Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Friday, August 13, 2010 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d on paraffin sections I need some advice. We have been trying to do C4d on paraffin sections without much success. Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much! Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Philip.Gibson <@t> nuth.nhs.uk Fri Aug 13 10:00:38 2010 From: Philip.Gibson <@t> nuth.nhs.uk (Gibson, Philip) Date: Fri Aug 13 10:01:50 2010 Subject: [Histonet] Acceptable way to "bake" sections onto slides? Message-ID: <1966D567F43F8A4AA975C2B46F830883074110@nuth-rex01.xnuth.nhs.uk> Hi Everyone Just for clarification; our current practice involves a mixture of placing racks of slides in an oven for 25 minutes, or using the hot area of an autostainer (we're talking H&E's here, BTW). Occasionally, with a particularly urgent case us old-timers will place individual slides directly onto the floor of an oven for 5 mins which bakes the sections onto the slides very nicely prior to staining. My theory is that a 60-70 celsius hotplate will perform the same job for all of our H&E slides. So far, I haven't noticed any particular artefacts, and we don't suffer from water pooling between section and slide anyway. I'm trying to find both the "leanest" and cheap (hey - this is the NHS!) solution by consolidating four stainers and three coverslippers into just the two machines. Maybe I've not looked closely enough at our super-urgent H&E's... Thanks Phil ---------------------------------------- Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net From wlecorch <@t> rwjuhh.edu Fri Aug 13 10:41:06 2010 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Fri Aug 13 10:41:12 2010 Subject: [Histonet] voice recognition Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71B73163EBB@HAMEXMBA.rwjham.local> We have been using Dragon with SoftPath and works well, there's a little bit of training the software at first but well worth it Bill Lecorchick Cytology Prep.Tech. 609-584-5128 Fax 609-584-6439 wlecorch@rwjuhh.edu www.rwjhamilton.org From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Aug 13 11:00:05 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Aug 13 11:00:22 2010 Subject: [Histonet] RE: voice recognition In-Reply-To: <09411E0112A96A459D8D5FBDAB9C15C71B73163EBB@HAMEXMBA.rwjham.local> References: <09411E0112A96A459D8D5FBDAB9C15C71B73163EBB@HAMEXMBA.rwjham.local> Message-ID: We use Dragon here as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lecorchick, William [wlecorch@rwjuhh.edu] Sent: Friday, August 13, 2010 11:41 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] voice recognition We have been using Dragon with SoftPath and works well, there's a little bit of training the software at first but well worth it Bill Lecorchick Cytology Prep.Tech. 609-584-5128 Fax 609-584-6439 wlecorch@rwjuhh.edu www.rwjhamilton.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Fri Aug 13 11:10:50 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Aug 13 11:10:56 2010 Subject: [Histonet] RELIA Histology Job Mohs Histotech Needed in Florida. Message-ID: Hi Histonetters!, How are you? I have a exciting new opportunity I want to tell you about. I am working with a leading client located in the Sarasota area. They are looking for someone to do Mohs histology. If you are interested you would also be considered for management of the lab. You would be working 4 10 hour days. They have great benefits - 3 weeks vacation, 100% paid medical benefits for the employee and 50% for family, and 100% paid dental for employee and family and a 401K. They are a busy fast paced lab and a great group of people to work with. If you think you might be interested please let me know. I will be in the office tomorrow -Saturday. You can reach me toll free at 866-607-3542 or shoot me an e-mail with a time to call you over the weekend or on Monday. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From maggie.allen <@t> nicewareintl.com Fri Aug 13 11:38:56 2010 From: maggie.allen <@t> nicewareintl.com (Maggie Allen) Date: Fri Aug 13 11:39:00 2010 Subject: [Histonet] Conferences / Trade Shows In-Reply-To: <20100813140506.CEC0FE80AC@spamfilter2.redanvil.net> References: <20100813140506.CEC0FE80AC@spamfilter2.redanvil.net> Message-ID: <6BCD4D0894AE0A4A96B4CB8F6779A57001E59C88@NICEWARE-MAIN03.NicewareIntl03.local> What conferences or trade shows do you attend and find most valuable? NSH? Pathology Informatics? Others? Maggie Allen Niceware International, LLC Tel? (810) 629-3930 Email: maggie.allen@nicewareintl.com From k84as <@t> yahoo.com Fri Aug 13 12:45:47 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Aug 13 12:45:51 2010 Subject: [Histonet] please enter Message-ID: <875537.15783.qm@web112609.mail.gq1.yahoo.com> Dear histonetters i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the opportunity to learn from it!! and we are ?ashamed? to talk about that with experts like you.but the group lose its functionality in teaching IHC for the new histologiest sector. so?i put our proplem on your hands and my hope is to help us to find solution ? an example of what i'm taking about is what is C4d and for what it is used? ? thanx in advance to your help and for these amazing group ? mohamed Faculty of Vet. Med. Cairo Univ. - Egypt ? ? ? We use the same antibody from Cell Marque on the Dako Autostainers with great success. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Sent: Friday, August 13, 2010 10:29 AM To: 'Martha Ward'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: C4d on paraffin sections We use the C4d from Cell Marque now with great results; it is an IVD. ER1 20 minutes on the BondMax and Bond III Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Friday, August 13, 2010 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d on paraffin sections I need some advice.? We have been trying to do C4d on paraffin sections without much success.? Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much!? Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Fri Aug 13 13:45:51 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Aug 13 13:45:59 2010 Subject: [Histonet] please enter In-Reply-To: <875537.15783.qm@web112609.mail.gq1.yahoo.com> References: <875537.15783.qm@web112609.mail.gq1.yahoo.com> Message-ID: Mohamed, Deposition of C4d is an inactive fragment of C4b (derived from complement component C4). It provides in-situ evidence of complement activation following alloantibody binding to graft vasculature. As such, the development of antibodies against C4d has dramatically improved the ability to diagnose antibody mediated rejection (AMR). We use the C4d (along with C3d) antibody from Cell Marque to investigate heart, lung and kidney biopsies for antibody mediated rejection in transplanted organs - in fact many consider C4d immunohistochemistry to be the gold standard for evaluation of AMR. C4d has also been postulated as a specific marker in the differential diagnosis of Follicular Lymphoma vs MALT lymphoma (Pathology - Research and Practice 2007; 203:163-167) Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, August 13, 2010 1:46 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] please enter Dear histonetters i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the opportunity to learn from it!! and we are ashamed to talk about that with experts like you.but the group lose its functionality in teaching IHC for the new histologiest sector. so i put our proplem on your hands and my hope is to help us to find solution an example of what i'm taking about is what is C4d and for what it is used? thanx in advance to your help and for these amazing group mohamed Faculty of Vet. Med. Cairo Univ. - Egypt We use the same antibody from Cell Marque on the Dako Autostainers with great success. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Sent: Friday, August 13, 2010 10:29 AM To: 'Martha Ward'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: C4d on paraffin sections We use the C4d from Cell Marque now with great results; it is an IVD. ER1 20 minutes on the BondMax and Bond III Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Friday, August 13, 2010 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d on paraffin sections I need some advice. We have been trying to do C4d on paraffin sections without much success. Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much! Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Fri Aug 13 13:57:43 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Aug 13 13:57:47 2010 Subject: [Histonet] voice recognition In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF05B0BBC967@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF05B0BBC967@EXCHANGESB> Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B757CA@exchange.cmc-nh.org> Carol we are using Dragon with a Voice Brook interface. Dragon will work by itself but has some issues with Pathology terminology. Voice Brook was created specifically for Pathology. Our staff, PA's and Pathologists really like this configuration. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, August 12, 2010 3:02 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] voice recognition Is anyone using voice recognition with SoftPath for dictating? If so, what software program are you using and does it work well? Thank you in advance for any comments. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Aug 13 14:38:50 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Aug 13 14:39:07 2010 Subject: [Histonet] CAP and RUO's Message-ID: <1AAF670737F193429070841C6B2ADD4C0255010019@EXMBMCB15.ucsfmedicalcenter.org> In case anyone is still wondering, here is the response from CAP to a question about their current position on use of RUO's for diagnostic use: "The CAP removed reference to RUOs in the checklist. The CAP position is RUOs are not to be used for clinical testing." Tim Morken UCSF Pathology San Francisco, CA From mtighe <@t> trudeauinstitute.org Fri Aug 13 15:26:51 2010 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Fri Aug 13 15:27:20 2010 Subject: [Histonet] Tissue-Tek VIP 2000-3000 Message-ID: <4C65723D.26E4.00EE.0@trudeauinstitute.org> I may have the chance to get a tissue processor (VIP 2000 or 3000). Are these units still serviceable? Is it hard to find parts? Are they Reliable? Thanks for any help!! Mike From rjbuesa <@t> yahoo.com Fri Aug 13 15:41:15 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 13 15:42:29 2010 Subject: [Histonet] Tissue-Tek VIP 2000-3000 In-Reply-To: <4C65723D.26E4.00EE.0@trudeauinstitute.org> Message-ID: <727330.95008.qm@web65707.mail.ac4.yahoo.com> I don't know the answer to your first 2 questions, but reliable they really are! Ren? J. --- On Fri, 8/13/10, Mike Tighe wrote: From: Mike Tighe Subject: [Histonet] Tissue-Tek VIP 2000-3000 To: histonet@lists.utsouthwestern.edu Date: Friday, August 13, 2010, 4:26 PM I may have the chance to get a tissue processor (VIP 2000 or 3000). Are these units still serviceable? Is it hard to find parts? Are they Reliable? Thanks for any help!! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Fri Aug 13 15:48:57 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Aug 13 15:49:05 2010 Subject: [Histonet] Tissue-Tek VIP 2000-3000 In-Reply-To: <727330.95008.qm@web65707.mail.ac4.yahoo.com> References: <4C65723D.26E4.00EE.0@trudeauinstitute.org> <727330.95008.qm@web65707.mail.ac4.yahoo.com> Message-ID: Agreed extremely reliable -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 13, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu; Mike Tighe Subject: Re: [Histonet] Tissue-Tek VIP 2000-3000 I don't know the answer to your first 2 questions, but reliable they really are! Ren? J. --- On Fri, 8/13/10, Mike Tighe wrote: From: Mike Tighe Subject: [Histonet] Tissue-Tek VIP 2000-3000 To: histonet@lists.utsouthwestern.edu Date: Friday, August 13, 2010, 4:26 PM I may have the chance to get a tissue processor (VIP 2000 or 3000). Are these units still serviceable? Is it hard to find parts? Are they Reliable? Thanks for any help!! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From lentwistle <@t> ucsd.edu Fri Aug 13 15:53:30 2010 From: lentwistle <@t> ucsd.edu (Entwistle, Laura) Date: Fri Aug 13 15:53:09 2010 Subject: [Histonet] cryostat cutting problems Message-ID: <0E51494EE610954592D9A04B07AAE35C135DA30378@MBX4.AD.UCSD.EDU> I am new to using a cryostat and am having some issues with my tissue. 1. Every slice seems to shatter and fragment. When I have used a microtome it was because the tissue was too cold. 2. The tissue itself was flash frozen and so was not fixed and the tissue did not go through the perfusion process. 3. Each slice is condensed and doesn't lay flat. 4. The cryostat is old. Any tips would be very helpful. From napoli <@t> siscom.net Fri Aug 13 15:57:33 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Fri Aug 13 15:57:37 2010 Subject: [Histonet] CF0145EBF1EB4C4E82768D82886A0C9B8FB06A@PLUTO.ad.murdoch.edu.au Message-ID: <4c65b1bd.1d.2fc2.1493031201@siscom.net> Joe "the toe" Nocito...are you out there? Joe has good ideas about nails. Maybe he will send out his procedure again. I like using either potassium hydroxide 10-20% or Sodium hydroxide 10-20% for softening nail fragments before processing. Also, keep in mind that soft tissues attached are equally as important and sections of nail beds need to be of high quality. A melanoma under a nail can be a bad situation. Sometimes in addition to PAS or GMS stains for onychomycosis, we have done melanin and iron stains for areas of pigment or hemmorhagic depositions. Joe...are you out there? lol From jnocito <@t> satx.rr.com Fri Aug 13 17:36:27 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Aug 13 17:36:54 2010 Subject: [Histonet] CF0145EBF1EB4C4E82768D82886A0C9B8FB06A@PLUTO.ad.murdoch.edu.au In-Reply-To: <4c65b1bd.1d.2fc2.1493031201@siscom.net> References: <4c65b1bd.1d.2fc2.1493031201@siscom.net> Message-ID: <56A23D5CD6C641FCB526615A554C5858@JoePC> Happy Friday all, try 10% sodium hydroxide for 1 hour prior to processing. It is important to let the slides drain really well before placing them in the oven. You want to make sure there is no water between the section and the slide. We use Plus slides with plain distilled water in our water baths. Hope this helps. JTT ----- Original Message ----- From: "Andrew Burgeson" To: Sent: Friday, August 13, 2010 3:57 PM Subject: [Histonet] CF0145EBF1EB4C4E82768D82886A0C9B8FB06A@PLUTO.ad.murdoch.edu.au > Joe "the toe" Nocito...are you out there? > > Joe has good ideas about nails. Maybe he will send out his > procedure again. > > > I like using either potassium hydroxide 10-20% or Sodium > hydroxide 10-20% for softening nail fragments before > processing. > > Also, keep in mind that soft tissues attached are equally as > important and sections of nail beds need to be of high > quality. A melanoma under a nail can be a bad situation. > Sometimes in addition to PAS or GMS stains for > onychomycosis, we have done melanin and iron stains for > areas of pigment or hemmorhagic depositions. > > Joe...are you out there? lol > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From graceofgod011978 <@t> hotmail.com Fri Aug 13 22:51:21 2010 From: graceofgod011978 <@t> hotmail.com (Emmanuel O) Date: Fri Aug 13 22:51:24 2010 Subject: [Histonet] Seeking Position in Canada Message-ID: I am a US trained and ASCP certified Histologist. I am looking for job opportunity in Canada either as Histologist or as an IHC Specialist. Thanking you all in advance. Emmanuel. From joelleweaver <@t> hotmail.com Sat Aug 14 06:42:11 2010 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Aug 14 06:42:15 2010 Subject: [Histonet] Conferences / Trade Shows In-Reply-To: <6BCD4D0894AE0A4A96B4CB8F6779A57001E59C88@NICEWARE-MAIN03.NicewareIntl03.local> References: <20100813140506.CEC0FE80AC@spamfilter2.redanvil.net>, <6BCD4D0894AE0A4A96B4CB8F6779A57001E59C88@NICEWARE-MAIN03.NicewareIntl03.local> Message-ID: I personally find the NSH conferences excellent as they are directed specifically to histology. I participate in CAP pathology informatics web courses, and other CAP sponsored webcast and web-delivered courses and find them timely and educational for histology practice, management and operations information. Some state histology groups, such as the one in Texas, provide very good webinars and teleconferences as well. Joelle Weaver HTL (ASCP) > Date: Fri, 13 Aug 2010 11:38:56 -0500 > From: maggie.allen@nicewareintl.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Conferences / Trade Shows > > What conferences or trade shows do you attend and find most valuable? > > NSH? Pathology Informatics? Others? > > > Maggie Allen > Niceware International, LLC > Tel (810) 629-3930 > Email: maggie.allen@nicewareintl.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Sat Aug 14 07:51:12 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Sat Aug 14 07:51:18 2010 Subject: [Histonet] RE: Acceptable way to "bake" sections onto slides? In-Reply-To: <1966D567F43F8A4AA975C2B46F83088307410E@nuth-rex01.xnuth.nhs.uk> References: <1966D567F43F8A4AA975C2B46F83088307410E@nuth-rex01.xnuth.nhs.uk> Message-ID: Better an extra step than inadequate baking. We put ours in an oven for 20 mins, cool with a fan and then put on our auto stainer. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gibson, Philip Sent: Friday, August 13, 2010 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Acceptable way to "bake" sections onto slides? Hi In our "fairly large" histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to "bake" for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil ---------------------------------------- Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From shultz11 <@t> cox.net Sat Aug 14 11:57:39 2010 From: shultz11 <@t> cox.net (shultz11@cox.net) Date: Sat Aug 14 11:57:41 2010 Subject: [Histonet] Histology Job Message-ID: <20100814125739.07BDJ.762727.imail@eastrmwml36> Full time histology position at Louisiana State University in Baton Rouge,LA. Please fill out application online at www.lsu.edu. Click on Human resouces, then job openings. Thanks From etambutte <@t> centrescientifique.mc Sat Aug 14 12:09:09 2010 From: etambutte <@t> centrescientifique.mc (Eric Tambutte) Date: Sat Aug 14 12:09:14 2010 Subject: [Histonet] Re: Histonet Digest, Vol 81, Issue 17 Message-ID: <1024366884@s15272523.onlinehome-server.info> Merci pour votre message. Je suis actuellement absent du laboratoire. Je repondrai a votre message a mon retour partir du 13 septembre 2010. En cas d'urgence merci de contacter : Prof. Denis Allemand allemand@centrescientifique.mc Thank you for your email. I am currently out of the lab. I will reply to your message after the 13th of September 2010. In case of emergency, please contact: Prof. Denis Allemand allemand@centrescientifique.mc From graceofgod011978 <@t> hotmail.com Sat Aug 14 17:37:24 2010 From: graceofgod011978 <@t> hotmail.com (Emmanuel O) Date: Sat Aug 14 17:37:28 2010 Subject: [Histonet] Seeking Position in Canada In-Reply-To: References: Message-ID: I am a US trained and ASCP certified Histologist. I am looking for job opportunity in Canada either as Histologist or as an IHC Specialist. Thanking you all in advance. Emmanuel. From amosbrooks <@t> gmail.com Sat Aug 14 22:23:45 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Aug 14 22:23:55 2010 Subject: [Histonet] please enter Message-ID: Hi, If you have a question the best thing to do is ask, so you are on the right track. I have three really good suggestions for you. First speak to your pathologists and try to learn as much as you possibly can from them about what they are looking for. This will familiarize you with what you need to know. Second, Read your antibody data sheets. Not just the parts of them that tell you how to do the procedure. Any (good) data sheet that should come with your antibodies will explain about the antibody, what it is, what it labels and which disease processes it is used for. Finally and very importantly use those autostainer companies until they get sick of you and then some more. (They like it when you do this ... really) They have some great literature that should help you. The new Cell Marque catalog is GREAT, it has images of each antibody on positive tissue, and great descriptions of each. DAKO has some of the best IHC guides out there. This is a link to a PDF of their Staining Methods Guide http://pri.dako.com/08002_ihc_staining_methods_5ed.pdf and they also have another really good book describing many clinically used antibodies with great images and descriptions. Unfortunately I can't recall the name of the book, if anyone can refresh my memory I'd appreciate it. There is a similar one for special stains. Now while I said three suggestions I'll toss in another anyway. The internet can be very helpful. Wikipedia can be useful for example in answer to your question about C4d, I found http://en.wikipedia.org/wiki/Complement_component_4 Have a great day, Amos Message: 1 Date: Fri, 13 Aug 2010 10:45:47 -0700 (PDT) From: mohamed abd el razik Subject: [Histonet] please enter To: Histonet@lists.utsouthwestern.edu Message-ID: <875537.15783.qm@web112609.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear histonetters i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the opportunity to learn from it!! and we are ashamed to talk about that with experts like you.but the group lose its functionality in teaching IHC for the new histologiest sector. so i put our proplem on your hands and my hope is to help us to find solution an example of what i'm taking about is what is C4d and for what it is used? thanx in advance to your help and for these amazing group mohamed Faculty of Vet. Med. Cairo Univ. - Egypt We use the same antibody from Cell Marque on the Dako Autostainers with great success. From talulahgosh <@t> gmail.com Sun Aug 15 11:43:34 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sun Aug 15 11:43:38 2010 Subject: [Histonet] cryostat cutting problems In-Reply-To: <0E51494EE610954592D9A04B07AAE35C135DA30378@MBX4.AD.UCSD.EDU> References: <0E51494EE610954592D9A04B07AAE35C135DA30378@MBX4.AD.UCSD.EDU> Message-ID: It may help to let your block equilibrate for about 20 minutes in the cryostat. I've found blocks do not section well if you start sectioning right away, especially snap frozen ones. Also, do you embed in OCT/sucrose? A 1:1 OCT: 30% sucrose solution is much softer than just OCT (or whatever embedding medium you are using). What do you mean by the tissue is condensed? It's folding up? I've found sectioning fresh unfixed tissue on a cryostat is impossible (we tried chick embryo trunks, which may be harder to section fresh than other tissue)--it doesn't section but get smushed to a pulp. You need to use a sledge microtome, unfortunately. What thickness are you sectioning at? I've found sectioning above sixty microns doesn't work as well on a cryostat, but again this is on fixed chick embryo tissue. If your cryostat is old, I would suggest you look for a new anti-roll plate made of glass. They work so much better. As long as your cryostat isn't older than about ten years, you should be able to find one that fits your knife holder very easily. That said, I've used a Rei-something cryostat that was 15 years old and it sucked. The anti-roll plate holder was too loose to do anything useful and the adjustment of the blade holder was impossible. Can you use a newer cryostat in your department from another lab? Or just go back to sledge microtome sectioning--if you know how to do it, it's the same principle anyway. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Fri, Aug 13, 2010 at 4:53 PM, Entwistle, Laura wrote: > I am new to using a cryostat and am having some issues with my tissue. > > > 1. Every slice seems to shatter and fragment. When I have used a > microtome it was because the tissue was too cold. > > 2. The tissue itself was flash frozen and so was not fixed and the > tissue did not go through the perfusion process. > > 3. Each slice is condensed and doesn't lay flat. > > 4. The cryostat is old. > > Any tips would be very helpful. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Sun Aug 15 11:47:17 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sun Aug 15 11:47:21 2010 Subject: [Histonet] please enter In-Reply-To: References: Message-ID: Please feel free to ask questions. I always do because I'm not in pathology (which most people here are) but neurobiology. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx From pruegg <@t> ihctech.net Sun Aug 15 12:26:40 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Aug 15 12:27:26 2010 Subject: SPAM-LOW: [Histonet] Staining Racks In-Reply-To: References: Message-ID: We managed to get racks from Leica that fit onto a different H&E stainer and the Leica coverslipper I would check with your Leica rep about this. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, August 06, 2010 7:24 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Staining Racks Hi, I recently acquired a used coverslipper (YEAY ME!!!) It is a Leica CV-5000. I know there are a number of big fans of this coverslipper here, but I have a possible problem. I also have an automatic slide stainer that I LOVE! The stainer is a Sakura DRS-2000. It has a slide rack holder that holds 2 Winlab 20 slide racks (the dusty charcoal colored racks). The Leica takes a rack that holds 30 or so slides with a hanger on either side. This of course doesn't fit the stainer or the stainer rack holder. So here's the conondrum, how do I get these instruments to play nicely together? Is there a rack holder that I could get for the Sakura stainer that would hold the Leica racks? Alternatively, is there any way to use the Winlab racks for the Sakura on the Leica coverslipper without having the slides jam? (coverslippers are ALL finicky) Are there any alternative solutions anyone has found? I had considered fabricating a stainer rack holder that would hold the Leica racks with plexiglass perhaps, but if there is a solution that is already being used I'd love to save the experimentation. I certainly won't transfer the slides between racks after staining. I might as well hand coverslip in that case. Thanks in advance for any help! Happy Friday, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Sun Aug 15 18:11:58 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 15 18:15:27 2010 Subject: [Histonet] please enter In-Reply-To: <875537.15783.qm@web112609.mail.gq1.yahoo.com> Message-ID: C4d Immunoreactivity has been used to assess transplant failure in kidneys and hearts Peritubular capillary deposition of C4d has been shown to be associated with both acute humoral and vascular rejection and increased graft loss (7,8). This was found to be independent of histological rejection type (7). Patients were considered C4d positive if 25% of the peritubular capillaries exhibited circumferential staining (7). Successful staining relies on Microwave antigen retrieval. Peritubular capillary C4d deposition in acute allograft rejection is a predictor of long term graft failure (7) and it has been suggested that these patients would benefit from intensive therapy, potentially preventing the previously reported high graft failure rate (8). 7. Herzenberg, A.M., Gill, J.S., Djurdjev, O., Magil, A.B., (2002) "C4d deposition in acute rejection: An Independent Long-term Prognostic factor" J Am Soc Nephrol 13(1):234-41. 8. Nickeleit, V., Zeeiler, M., Gudat, F., Theil, G., Mihatsch, M.J., (2002) "Detection of the complement degradation product C4d in renal allografts: diagnostic and therapeutic implications: J Am Soc Nephrol 13(1):242-51 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Saturday, 14 August 2010 3:46 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] please enter Dear histonetters i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the opportunity to learn from it!! and we are ?ashamed? to talk about that with experts like you.but the group lose its functionality in teaching IHC for the new histologiest sector. so?i put our proplem on your hands and my hope is to help us to find solution ? an example of what i'm taking about is what is C4d and for what it is used? ? thanx in advance to your help and for these amazing group ? mohamed Faculty of Vet. Med. Cairo Univ. - Egypt ? ? ? We use the same antibody from Cell Marque on the Dako Autostainers with great success. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Sent: Friday, August 13, 2010 10:29 AM To: 'Martha Ward'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: C4d on paraffin sections We use the C4d from Cell Marque now with great results; it is an IVD. ER1 20 minutes on the BondMax and Bond III Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Friday, August 13, 2010 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d on paraffin sections I need some advice.? We have been trying to do C4d on paraffin sections without much success.? Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much!? Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From AnthonyH <@t> chw.edu.au Sun Aug 15 18:29:39 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 15 18:33:00 2010 Subject: [Histonet] RE: Histonet Digest, Vol 80, Issue 31 In-Reply-To: Message-ID: Is this what you are looking for? Ng et al Re-hydration Method for Red Blood Cell Lysis Principle: R-ehydration of air dried smears with normal saline for 30 seconds before fixation in alcohol has been found to produce slides of a quality superior or at least equivalent to that of immediate fixed smears. It allowed the cells to adhere better to the slide and lysis of red blood cells provided a clean background for better assessment. Solutions: 1. Normal Saline Sodium Chloride 9g Distilled water 1000ml 2. 95% Ethanol Procedure: 1. After smearing material, air dry slides using a hair dryer. 2. Place slides in Normal Saline for 30sec. 3. Immediately place slides in 95% ethanol and fix for 10 minutes. 4. Stain slides via PAP. Results: RBCs were effectively lysed but epithelial and mesothelial cells were retained. In general, smears showed a decrease in chromaticity of both nuclear and cytoplasmic staining. Cytoplasmic vacuoles were less distinct and epithelial fragments, such as acini, appeared flattened and could be more easily focussed on the same plane. Nuclear and cellular moulding was exaggerated and more appreciable when present. There was a slight enlargement of cells. Degenerated specimens, even in the absence of polymorphs, often resulted in disappointing re-hydrated smears. Cell clusters were easier to see probably because of the higher transparency of the cytoplasm. Reference: Acta Cytolog (1994) 38(1):56-64. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Aazath Raj Sent: Thursday, 12 August 2010 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 80, Issue 31 Dear Friends, Does anyone have rehydration technique of air dried smear which can be stained for pap. Aazathraj.P Technical Officer Department of Histopathology and Cytology Apollo Hospitals -chennai India _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From AnthonyH <@t> chw.edu.au Sun Aug 15 18:33:21 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 15 18:36:41 2010 Subject: [Histonet] PAP In-Reply-To: Message-ID: This is our method: 1. Tap water dip gently 5-10 times 2. Harris haematoxylin 5 minutes 3. Wash in running water 4. Differentiate in acid/alcohol one dip 5. Wash in running water 6. Scott's Blueing Solution one minute 7. Wash in running water. 8. 95% Alcohol dip gently 5-10 times 9. Modified PAP Stain (see below) three minutes 10. Rinse excess dye off in 95% Alcohol 11. Absolute Alcohol dip gently 5-10 times 12. Absolute Alcohol dip gently 5-10 times 13. Xylol dip gently 5-10 times 14. Xylol dip gently 5-10 times 15. Xylol dip gently 5-10 times 16. Mount in D.P.X. Mounting Medium. This modification uses a single solution, omitting the OG6 solution and replacing the light green with the more fade resistant Fast Green FCF. Modified PAP stain Stock solutions: Prepare 10% solutions of each of the stains as follows: 2.5g Eosin Y (CI 45380) in 25ml distilled water 1g Fast Green FCF (CI 42053) in 10ml distilled water Working Solution: Mix (for 400ml stain) 12ml Eosin Y stock 2.5ml Fast Green FCF Stock Make mixture up to 400ml with 95% alcohol. Add: 0.8g Phosphotungstic acid 4 drops saturated lithium carbonate. Mix well. Store solution in dark brown, tightly capped bottle. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Aazath Raj Sent: Thursday, 12 August 2010 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAP Dear friends, does anyone have rapid PAP(cytostain) preparation,for rapid PAP staining. Aazathraj.P Technical Officer Department of Histopathology and Cytology Apollo Hospitals chennai India _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From bstephen <@t> fastmail.fm Mon Aug 16 05:44:50 2010 From: bstephen <@t> fastmail.fm (Birgitta Stephenson) Date: Mon Aug 16 05:44:55 2010 Subject: [Histonet] Re: test animal vs. plant In-Reply-To: <1281566852.7065.15.camel@chico.322tulane.org> References: <1279793945.4143.1386127399@webmail.messagingengine.com> <1281566852.7065.15.camel@chico.322tulane.org> Message-ID: <1281955490.23466.1390109961@webmail.messagingengine.com> Thanks Michael, I have discovered that the neagtive Phloroglucinol result does not eliminate plant as you pointed out. With the Phloroglucinol being light sensitive I have been able to conter stain with the Toluidine Blue which then stains non lignin plant tissue as well as lignin so works as a further line of evidence. I have had to play with the dilutions as the Toluidine Blue requires tap water to be added which I can not remove easily without losing a lot of the residue lifted. I have not looked at the fluoresing dyes but it is definately on the agenda. Any more thoughts most welcome. Thank you again. Regards Birgitta Archaeology Microscopy Lab University of Queensland On Wed, 11 Aug 2010 16:47:32 -0600, "Michael Folsom" said: > > With phloroglucinol you are staining for lignins which can be present in > varying abouts in plant tissue. Also, please note that different types > of lignins occur in different types of plants at different levels so > sadly negative results don't mean much. > > Other carbos to consider are cellulose or callose - calcoflour white is > an easy to use stain (0.1% in dH2O) for cellulose and flouresces in the > UV range, aniline blue stains callose also in the UV range. > > I know none of these are diagnostic by themselves but if you tie it to > some macerates and see fibers or trachieds then you have some plant > tissue. > > Mike > > Rio Grande Biological > Albuquerque, NM > > > On Thu, 2010-07-22 at 20:19 +1000, Birgitta Stephenson wrote: > > Hello Tamara, > > > > Thanks for your reply. In the lab we have worked with the carbohydrate > > ideas, so travelling down the same path. With the grindstones we are > > looking for some tests that we can also carry out in the field as > > sometimes we have grinding hollows or stones in situ which can not be > > moved. This is why we thought simple visual staining and utilising a > > hand held digital microscope could be the answer. However the staining > > is not as straight forward as we'd hoped. I am thinking that if the > > Phloroglucinol is light sensitive then perhaps counterstaining with > > Toluidine Blue can highlight other areas. Problem is the residues are > > water lifted and drowning the slide with tap water (well drops of) means > > that the residue at times almost vanishes. Any more thoughts are most > > welcome. > > > > Thnaks again > > > > Birgitta Stephenson > > > > Research Microscopy Lab, University of Queensland > > On Tue, 20 Jul 2010 11:41:51 -0600, "Tamara A Howard" > > said: > > > I saw your post on the Histonet - it seems that there must > > > specific tests that could do what you need. I Googled the > > > word string: "test distinguish plant animal" & got the > > > following promising hit: > > > > > > http://www.hsc.csu.edu.au/chemistry/options/forensic/2964/ch992nov03.html > > > > > > My guess is that there would be more info from forensic > > > science sites; they must have listservers, too. > > > > > > It sounds like a very interesting project - I'm envious! > > > > > > Tamara > > > > > > *************************** > > > Tamara Howard > > > Cell Biology & Physiology > > > UNM-HSC > > > Albuquerque, NM > > > *************************** > > -- > > Birgitta Stephenson > > bstephen@fastmail.fm > > > > -- Birgitta Stephenson bstephen@fastmail.fm -- http://www.fastmail.fm - Accessible with your email software or over the web From Cathy.Crumpton <@t> tuality.org Mon Aug 16 10:41:01 2010 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Mon Aug 16 10:41:07 2010 Subject: [Histonet] xylene in an unventilated area Message-ID: Hello all. Cytology was told that the xylene substitute the using for their Surepath paps was causing staining/artifact issues.& nbsp; They want to switch to using xylene in their area, but it is unventil moved.&nbs control. I handle the xylen uncovered while covers similar set up? The others are complaining about mini-hood from us. Badge resu Cathy Crumpton HT(ASC Tuality Community Hospital Hillsboro, OR 97123 From rjbuesa <@t> yahoo.com Mon Aug 16 11:59:56 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 16 12:00:00 2010 Subject: [Histonet] xylene in an unventilated area In-Reply-To: Message-ID: <196652.48038.qm@web65707.mail.ac4.yahoo.com> Try to get a fume hood; it could be one movible over the counter, but needs to have a good charcoal filter. Ren? J. --- On Mon, 8/16/10, Cathy.Crumpton@tuality.org wrote: From: Cathy.Crumpton@tuality.org Subject: [Histonet] xylene in an unventilated area To: histonet@lists.utsouthwestern.edu Date: Monday, August 16, 2010, 11:41 AM ???Hello? all.???Cytology was told that the xylene substitute the= y were ???using? for their Surepath paps was causing staining/artifact issues.&???nbsp;? They? want? to? switch to using xylene in their area, but it is ???unventil=? ated.? Because of various reasons, their process can not be ???moved.&nbs= p; They are asking me for advice about what to do for fume ???control.? I= was wondering if a good downdraft unit would be enough to ???handle? the? xylen=? e? fumes.???They will have maybe 400 mL of xylene ???uncovered? while? covers= lipping their paps.? Does anyone else have a ???similar? set? up?? The= y share their area with micro and core lab and ???others? are? complaining about= fumes even though they have borrowed a ???mini-hood from us.? Badge resu= lts are still pending. ???Cathy Crumpton HT(ASC= P), Histology Lead ???Tuality Community Hospital ???Hillsboro, OR 97123 ???= (503)681-1292 ???_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHargrove <@t> urhcs.org Mon Aug 16 12:32:00 2010 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Mon Aug 16 12:32:06 2010 Subject: [Histonet] Nail Problems /Equipment In-Reply-To: Message-ID: I am replying to both posts, getting the section of the nail used to be the toughest thing to do,now that I use the Sodium Hydroxide soak it seems that I have less problems keeping the sections on. To help that issue, after cutting and drying in the oven for 10 minutes, I put the slide in a Coplin jar with about 1cc of formaldehyde.Put the lid on loosely . Leave this in the oven 1-2- ours and then stain. About the instruments , we have a Ventana Benchmark XT. It works fine , we just upgraded to the Ultra for continuous load and do not have room for both in the Lab. We do still use it for routine IHC, and was last serviced in February this year. (Embedded image moved to file: pic17752.jpg) From youngir <@t> gmail.com Mon Aug 16 12:55:29 2010 From: youngir <@t> gmail.com (Ian Young) Date: Mon Aug 16 12:55:34 2010 Subject: [Histonet] p63 Message-ID: Histonet, I had read about a week ago about someone asking about the rights to sell p63. I did some research and hope this helps: http://www.histobob.com/?q=node/121 Thanks, Ian From MSHERWOOD <@t> PARTNERS.ORG Mon Aug 16 13:27:28 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Aug 16 13:26:56 2010 Subject: [Histonet] metal base molds In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2456E@PHSXMB30.partners.org> Thanks to all who answered my request. I checked out EMS and Ted Pella, but found the best size/best price right in my "own backyard"--Brain Research Lab in Newton, MA. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, August 12, 2010 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] metal base molds The following link has larger molds, maybe one of them will work. http://www.brainresearchlab.com/productinform.html Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 Message: 20 Date: Thu, 12 Aug 2010 11:51:37 -0400 From: "Sherwood, Margaret " Subject: Re: [Histonet] metal base molds for paraffin embedding To: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24554@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" To all: We need to order metal base molds for paraffin embedding. We need a size @45mm(L) X 32mm(W) X 11mm(D). (We have some, but have no idea where and when we ordered them). I have looked in several catalogs but can only find a depth of 5mm. The depth is the important part. If someone knows where I can order these, I would appreciate it. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From camayton <@t> gmail.com Mon Aug 16 13:38:49 2010 From: camayton <@t> gmail.com (Cathy Mayton) Date: Mon Aug 16 13:38:53 2010 Subject: [Histonet] attention equipment resellers/refurbishers Message-ID: I have a 6 foot external exhaust fume hood with blower that I need to find a home for. It has a fairly large sink in the left side of the countertop and 1/2 of the base cabinet is for flammable storage. If interested please contact me personally at camayton@gmail.com. I can send pictures to interested parties. -- Cathy Mayton From timwdrury <@t> gmail.com Mon Aug 16 14:24:56 2010 From: timwdrury <@t> gmail.com (Timothy Drury) Date: Mon Aug 16 14:25:00 2010 Subject: [Histonet] oh my god-j Message-ID: hey my god : I bought 5 products Apple iPhone 4G HD 16GB Black website: dhsellso.com ,to my surprise! it's genuine. much cheaper.You can check it! Hope all is going well for you. 8 From Sandra.Harrison3 <@t> va.gov Mon Aug 16 14:51:44 2010 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Mon Aug 16 14:52:58 2010 Subject: [Histonet] QC on stained slides In-Reply-To: References: Message-ID: You have included all the criteria; fixation, processing, embedding, microtomy, staining, coverslipping and labeling. If you were able to submit slides to CAP under their HQIP program, you would get graded evaluations. Short of that, could you perhaps send out duplicate slides to another local lab, or sister hospital, for peer review? You could offer to exchange slides on a twice yearly basis, since they, too, may be looking for additional quality control. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, August 02, 2010 2:25 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] QC on stained slides Hi all As part of a self assessment programme conducted by my employer, and related to my performance review and salary adjustment, I need to determine the criteria of what makes a stained slide acceptable or unacceptable. I was wondering if anyone out there had a "checklist" that they would be willing to share, that i could perhaps adapt. I realise that the easiest would be to send slides out for external control, but in this case it is not feasible. What I put together is this: - Quality of decalcification, processing, infiltration - Quality of sections (no wrinkles, missing bits, scores etc) - Entire representation of tissue area - staining pattern as expected according to protocol - coverslipped without bubbles or other inclusions - labelled neatly and correctly but, the question inmy mind is what would be the criteria that would make a slide merely adequate or truely outstanding? PLease help thank you -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jseaton <@t> wlgore.com Mon Aug 16 18:02:31 2010 From: jseaton <@t> wlgore.com (Janella Seaton) Date: Mon Aug 16 18:02:37 2010 Subject: [Histonet] AUTO: Janella Seaton/WLGORE is out of the office. (returning 08/23/2010) Message-ID: I am out of the office until 08/23/2010. I will respond to your message when I return. requests or questions can be sent to: histology@ Note: This is an automated re t, Vol 81, Issue 19" This is the only notification away. From Gervaip <@t> aol.com Mon Aug 16 21:43:14 2010 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Mon Aug 16 21:43:36 2010 Subject: [Histonet] xylene in an unventilated area Message-ID: I just recently did a lot of research into the different hoods. I decided the down draft was not going to work for us since we like to place absorbant towels down to catch the xylene while coverslipping. I decided to purchase one that a high CFM (Air flow rate), low decibles and a monitor on the filter to let one know when the filter was saturated and needed to be changed. And I wanted the sash that comes down in front of your face to be glass, so that we could clean it periodically with xylene to get splashes of mounting media off. For the money PurAir was very good... body was plastic and the clear part that is lowered while in use was plastic. It had good CFM, good decible level and a monitor for filter saturation. The LabConco is stainless steel and glass, with good CFM, decible level and a monitor for filter saturation. The problem with the little Shandon is that the CFM is about 30 at max when the sash is lowered. Newer ones, such as the PurAir and Labconco have about 120+ CFM. And the Shandon has no monitor for filter saturation. The PurAir had the footprint needed for cytology, which is very small in my area. There is another company that was good, but my brain cells cannot recall the info. If anyone wants more info, please e-mail me. In a message dated 8/16/2010 12:01:08 P.M. Central Daylight Time, rjbuesa@yahoo.com writes: Try to get a fume hood; it could be one movible over the counter, but needs to have a good charcoal filter. Ren? J. --- On Mon, 8/16/10, Cathy.Crumpton@tuality.org wrote: From: Cathy.Crumpton@tuality.org Subject: [Histonet] xylene in an unventilated area To: histonet@lists.utsouthwestern.edu Date: Monday, August 16, 2010, 11:41 AM Hello all. Cytology was told that the xylene substitute the= y were using for their Surepath paps was causing staining/artifact issues.& nbsp; They want to switch to using xylene in their area, but it is unventil= ated. Because of various reasons, their process can not be moved.&nbs= p; They are asking me for advice about what to do for fume control. I= was wondering if a good downdraft unit would be enough to handle the xylen= e fumes. They will have maybe 400 mL of xylene uncovered while covers= lipping their paps. Does anyone else have a similar set up? The= y share their area with micro and core lab and others are complaining about= fumes even though they have borrowed a mini-hood from us. Badge resu= lts are still pending. Cathy Crumpton HT(ASC= P), Histology Lead Tuality Community Hospital Hillsboro, OR 97123 = (503)681-1292 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ejschmid <@t> ucalgary.ca Tue Aug 17 10:40:48 2010 From: ejschmid <@t> ucalgary.ca (ejschmid@ucalgary.ca) Date: Tue Aug 17 10:41:05 2010 Subject: [Histonet] staining chick ectoderm Message-ID: <2f7955a13c473913341dd97bcc54e965.squirrel@webmail.ucalgary.ca> Hello, Question about was to specifically wholemount stain embryonic chick ectoderm, leaving underlying mesenchyme unstained. It has been suggested I try EtBr to stain the ecotderm in this way. Will this work? Seems like it would penetrate into the mesenchyme as well. Thanks. Eric From integrated.histo <@t> gmail.com Tue Aug 17 11:23:37 2010 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Tue Aug 17 11:23:41 2010 Subject: [Histonet] Formula 83 Message-ID: We have 2 cases of CBG's Formula 83. We would like to find it a home instead of dumping it as hazardous waste. Our lab is closing so we can no longer use it. If you would like it and are willing to set up and pay for shipping it is yours. Cindy DuBois Integrated Pathology Stockton, CA From mucram11 <@t> comcast.net Tue Aug 17 13:53:56 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Tue Aug 17 13:54:00 2010 Subject: [Histonet] Ventana Vantage System In-Reply-To: <158651132.65681.1282071086482.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <1197184412.65760.1282071236407.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Good Afternoon, We are having a demo of this system in teh next week in the form of a presentation.? I am interested in knowing more about the system along with the pros and cons of how it may work in a real lab.? If you could share any information or pros and cons it would be appreciated.? I would prefer going inot this meeting with more than a picture. Thank You, Pam Marcum UAMS Little Rock, AR From Sharon.Davis-Devine <@t> carle.com Tue Aug 17 14:16:26 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Tue Aug 17 14:16:31 2010 Subject: [Histonet] Microtome alignment Message-ID: We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From malbenatti <@t> gmail.com Tue Aug 17 14:33:40 2010 From: malbenatti <@t> gmail.com (Malika Benatti) Date: Tue Aug 17 14:33:47 2010 Subject: [Histonet] H1B Visa Sponsorship Message-ID: I wonder if anyone can help or perhaps knows anyone who could. I am a UK trained and registered medical technologist and have specialised in Histology, with 8 years of experience. I am fully registered with theHealth Professional Council. I am looking for a H1B Visa Sponsorship opportunity inTennessee/Knoxville or surrounding areas. I wonder if anyone can help or would be interested in having a informal discussion. I will be inKnoxville between the 20th of September until the 2nd of October and would be more then happy to travel and meet for an informal interview. Malika From vgrover <@t> polysciences.com Tue Aug 17 14:53:10 2010 From: vgrover <@t> polysciences.com (Valantou Grover) Date: Tue Aug 17 14:53:18 2010 Subject: [Histonet] FW: NEEDS URGENT HELP !!! Message-ID: <67097D5756B74E3080DAC19A958FC62C@USWARD13ZFB71> I am just curious, can someone check on Jerry to see if he is dead or alive, or if someone stole his phone/laptop to get this message to me. I remember the email address from Florida Society for Histotechnology, I am wondering if this is real or if someone is assuming his identity overseas, foul play, etc. Valantou Grover, Ph.D. HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-343-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 **************************************************************************** ************** * Visit us at the 36th Annual National Society for Histotechnology Convention - September 26-28, Seattle, WA - BOOTH 126-128 **************************************************************************** **************** * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex Course in Orlando, FL at the Grand Floridian Resort from 10/3 - 10/5. Please enquire at info@bangslabs.com for additional details! Phone: 800-387-0672, 317-570-7020. **************************************************************************** ***************** _____ From: JERRY SANTIAGO [mailto:santij1@bellsouth.net] Sent: Tuesday, August 17, 2010 1:53 PM To: undisclosed recipients: Subject: NEEDS URGENT HELP !!! Help! I'm sorry to be emailing you like this, but I am in need of some urgent assistance. As you have know, I've been traveling through Europe. But last night in Scotland, my worst fears came true. As we were headed back to the hotel, we were robbed but a crazy man with a knife. He took our bags, wallets, and phones. We have no credit cards or cash.Thankfully, we had left our passports in the safe at the hotel. We need to get back home, but we have no way to get to our flight in Germany that leaves for home next week. Right now our only option is to fly home from here. We need some money so we can purchase tickets to get to JFK, and then fly home from there. As soon as we get back home, I can repay you, I just need to get some cash to get there. If you can help, send me an email back, we have internet access from the lobby in the hotel. I'm sorry to bother you, I just didn't know how else to contact everyone quickly Thanks! From brent.jeter <@t> gwu-hospital.com Tue Aug 17 15:01:47 2010 From: brent.jeter <@t> gwu-hospital.com (Jeter, Brent) Date: Tue Aug 17 15:05:29 2010 Subject: [Histonet] FW: NEEDS URGENT HELP !!! In-Reply-To: <67097D5756B74E3080DAC19A958FC62C@USWARD13ZFB71> References: <67097D5756B74E3080DAC19A958FC62C@USWARD13ZFB71> Message-ID: This is an internet scam attempt. One of our staff received the same email, allegedly from her nephew. Someone has apparently hacked into Jerry's email account and sent this to addresses in his contact list. Brent Jeter Anatomic Pathology Supervisor The George Washington University Hospital 202-715-5076 (phone) 202-715-4691 (fax) brent.jeter@gwu-hospital.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Valantou Grover [vgrover@polysciences.com] Sent: Tuesday, August 17, 2010 3:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: NEEDS URGENT HELP !!! I am just curious, can someone check on Jerry to see if he is dead or alive, or if someone stole his phone/laptop to get this message to me. I remember the email address from Florida Society for Histotechnology, I am wondering if this is real or if someone is assuming his identity overseas, foul play, etc. Valantou Grover, Ph.D. HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-343-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 **************************************************************************** ************** * Visit us at the 36th Annual National Society for Histotechnology Convention - September 26-28, Seattle, WA - BOOTH 126-128 **************************************************************************** **************** * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex Course in Orlando, FL at the Grand Floridian Resort from 10/3 - 10/5. Please enquire at info@bangslabs.com for additional details! Phone: 800-387-0672, 317-570-7020. **************************************************************************** ***************** _____ From: JERRY SANTIAGO [mailto:santij1@bellsouth.net] Sent: Tuesday, August 17, 2010 1:53 PM To: undisclosed recipients: Subject: NEEDS URGENT HELP !!! Help! I'm sorry to be emailing you like this, but I am in need of some urgent assistance. As you have know, I've been traveling through Europe. But last night in Scotland, my worst fears came true. As we were headed back to the hotel, we were robbed but a crazy man with a knife. He took our bags, wallets, and phones. We have no credit cards or cash.Thankfully, we had left our passports in the safe at the hotel. We need to get back home, but we have no way to get to our flight in Germany that leaves for home next week. Right now our only option is to fly home from here. We need some money so we can purchase tickets to get to JFK, and then fly home from there. As soon as we get back home, I can repay you, I just need to get some cash to get there. If you can help, send me an email back, we have internet access from the lobby in the hotel. I'm sorry to bother you, I just didn't know how else to contact everyone quickly Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. From akemiat3377 <@t> yahoo.com Tue Aug 17 15:14:28 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue Aug 17 15:14:37 2010 Subject: Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! In-Reply-To: <67097D5756B74E3080DAC19A958FC62C@USWARD13ZFB71> References: <67097D5756B74E3080DAC19A958FC62C@USWARD13ZFB71> Message-ID: The email you received is a Nigerian Scam. Someone was able to hack into Jerry's computer and get all his contact list! You happened to be one of his contacts. The same sort of Scam happened to me last October. Fortunately for me, I didn't have any bank account information in my computer. I have a MAC and so I went to the Apple Genius Bar and they checked my computer for any viruses. Fortunately, it is very difficult to get into a MAC. This has been on the news in Idaho and various other states. DO NOT RESPOND to any emails that want any personal information!!! You need to clean-up your computer from any viruses. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Aug 17, 2010, at 12:53 PM, Valantou Grover wrote: > I am just curious, can someone check on Jerry to see if he is dead > or alive, > or if someone stole his phone/laptop to get this message to me. I > remember > the email address from Florida Society for Histotechnology, I am > wondering > if this is real or if someone is assuming his identity overseas, > foul play, > etc. > > > > Valantou Grover, Ph.D. > > HT/HTL(ASCP), PA, MBA > > Biosciences Product Line Manager > > Polysciences, Inc. > > 400 Valley Road > > Warrington, PA 18976 > > Fax: 1-800-343-3291 > > Phone number: 1-800-523-2575 X7418 > > Direct:1-215-488-7418 > > Cell phone: 1-215-409-8327 > > ********************************************************************** > ****** > ************** > > * Visit us at the 36th Annual National Society for Histotechnology > Convention - September 26-28, Seattle, WA - BOOTH 126-128 > > ********************************************************************** > ****** > **************** > > * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex > Course in Orlando, FL at the Grand Floridian Resort from 10/3 - 10/5. > Please enquire at info@bangslabs.com for additional details! Phone: > 800-387-0672, 317-570-7020. > > ********************************************************************** > ****** > ***************** > > _____ > > From: JERRY SANTIAGO [mailto:santij1@bellsouth.net] > Sent: Tuesday, August 17, 2010 1:53 PM > To: undisclosed recipients: > Subject: NEEDS URGENT HELP !!! > > > > > > Help! I'm sorry to be emailing you like this, but I am in need of some > urgent assistance. As you have know, I've been traveling through > Europe. > But last night in Scotland, my worst fears came true. As we were > headed back > to the hotel, we were robbed but a crazy man with a knife. He took > our bags, > wallets, and phones. We have no credit cards or cash.Thankfully, we > had left > our passports in the safe at the hotel. We need to get back home, > but we > have no way to get to our flight in Germany that leaves for home > next week. > > Right now our only option is to fly home from here. We need some > money so we > can purchase tickets to get to JFK, and then fly home from there. > As soon as > we get back home, I can repay you, I just need to get some cash to get > there. If you can help, send me an email back, we have internet > access from > the lobby in the hotel. I'm sorry to bother you, I just didn't know > how else > to contact everyone quickly Thanks! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Tue Aug 17 15:37:47 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue Aug 17 15:39:01 2010 Subject: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! In-Reply-To: References: <67097D5756B74E3080DAC19A958FC62C@USWARD13ZFB71> Message-ID: I am rethinking this email since I read it over again. The scam email I received asked for money ($1,800) to be sent to England via Western Union. I am not sure about this since this supposed Jerry is asking for just a response email. I would still be very cautious! The Idaho news said the lady received a "yahoo request" (same as mine) asking for email address, password, country of origin, mothers maiden name, ect. Once the perpetrator had the information, they immediately extracted all her information and cleaned her bank accounts out. This was a business computer account and it shut down her business! Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Aug 17, 2010, at 1:20 PM, Valantou Grover wrote: > Thanks, I deleted the message, now I am going to run a anti virus > program. > > Valantou > ********************************************************************** > ******************** > * Visit us at the 36th Annual National Society for Histotechnology > Convention - September 26-28, Seattle, WA - BOOTH 126-128 > ********************************************************************** > ********************** > * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 > Latex Course in Orlando, FL at the Grand Floridian Resort from 10/3 > ? 10/5. Please enquire at info@bangslabs.com for additional > details! Phone: 800-387-0672, 317-570-7020. > ********************************************************************** > *********************** > From: Akemi Allison [mailto:akemiat3377@yahoo.com] > Sent: Tuesday, August 17, 2010 4:14 PM > To: Valantou Grover > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! > > The email you received is a Nigerian Scam. Someone was able to > hack into Jerry's computer and get all his contact list! You > happened to be one of his contacts. The same sort of Scam happened > to me last October. Fortunately for me, I didn't have any bank > account information in my computer. I have a MAC and so I went to > the Apple Genius Bar and they checked my computer for any viruses. > Fortunately, it is very difficult to get into a MAC. This has been > on the news in Idaho and various other states. DO NOT RESPOND to > any emails that want any personal information!!! You need to clean- > up your computer from any viruses. > > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > On Aug 17, 2010, at 12:53 PM, Valantou Grover wrote: > > > I am just curious, can someone check on Jerry to see if he is dead > or alive, > or if someone stole his phone/laptop to get this message to me. I > remember > the email address from Florida Society for Histotechnology, I am > wondering > if this is real or if someone is assuming his identity overseas, > foul play, > etc. > > > > Valantou Grover, Ph.D. > > HT/HTL(ASCP), PA, MBA > > Biosciences Product Line Manager > > Polysciences, Inc. > > 400 Valley Road > > Warrington, PA 18976 > > Fax: 1-800-343-3291 > > Phone number: 1-800-523-2575 X7418 > > Direct:1-215-488-7418 > > Cell phone: 1-215-409-8327 > > ********************************************************************** > ****** > ************** > > * Visit us at the 36th Annual National Society for Histotechnology > Convention - September 26-28, Seattle, WA - BOOTH 126-128 > > ********************************************************************** > ****** > **************** > > * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex > Course in Orlando, FL at the Grand Floridian Resort from 10/3 - 10/5. > Please enquire at info@bangslabs.com for additional details! Phone: > 800-387-0672, 317-570-7020. > > ********************************************************************** > ****** > ***************** > > _____ > > From: JERRY SANTIAGO [mailto:santij1@bellsouth.net] > Sent: Tuesday, August 17, 2010 1:53 PM > To: undisclosed recipients: > Subject: NEEDS URGENT HELP !!! > > > > > > Help! I'm sorry to be emailing you like this, but I am in need of some > urgent assistance. As you have know, I've been traveling through > Europe. > But last night in Scotland, my worst fears came true. As we were > headed back > to the hotel, we were robbed but a crazy man with a knife. He took > our bags, > wallets, and phones. We have no credit cards or cash.Thankfully, we > had left > our passports in the safe at the hotel. We need to get back home, > but we > have no way to get to our flight in Germany that leaves for home > next week. > > Right now our only option is to fly home from here. We need some > money so we > can purchase tickets to get to JFK, and then fly home from there. > As soon as > we get back home, I can repay you, I just need to get some cash to get > there. If you can help, send me an email back, we have internet > access from > the lobby in the hotel. I'm sorry to bother you, I just didn't know > how else > to contact everyone quickly Thanks! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ehill2 <@t> partners.org Tue Aug 17 15:47:57 2010 From: ehill2 <@t> partners.org (Eric Hill) Date: Tue Aug 17 15:48:07 2010 Subject: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! In-Reply-To: References: <67097D5756B74E3080DAC19A958FC62C@USWARD13ZFB71> Message-ID: Hi, My wife got scammed with something almost the same a month ago, less detailed message in hers though! They got into her gmail and deleted all her contacts and tried to delete all her most recent emails (they forgot to clear out her trash though, so she got most back). Don't fall for it! -Eric Research Tech 1 Program in Memrane Biology Mass Genreal Hospital On Aug 17, 2010, at 4:37 PM, Akemi Allison wrote: > I am rethinking this email since I read it over again. The scam email I received asked for money ($1,800) to be sent to England via Western Union. I am not sure about this since this supposed Jerry is asking for just a response email. I would still be very cautious! > > The Idaho news said the lady received a "yahoo request" (same as mine) asking for email address, password, country of origin, mothers maiden name, ect. Once the perpetrator had the information, they immediately extracted all her information and cleaned her bank accounts out. This was a business computer account and it shut down her business! > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > On Aug 17, 2010, at 1:20 PM, Valantou Grover wrote: > >> Thanks, I deleted the message, now I am going to run a anti virus program. >> >> Valantou >> ****************************************************************************************** >> * Visit us at the 36th Annual National Society for Histotechnology Convention - September 26-28, Seattle, WA - BOOTH 126-128 >> ******************************************************************************************** >> * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex Course in Orlando, FL at the Grand Floridian Resort from 10/3 ? 10/5. Please enquire at info@bangslabs.com for additional details! Phone: 800-387-0672, 317-570-7020. >> ********************************************************************************************* >> From: Akemi Allison [mailto:akemiat3377@yahoo.com] >> Sent: Tuesday, August 17, 2010 4:14 PM >> To: Valantou Grover >> Cc: histonet@lists.utsouthwestern.edu >> Subject: Re: Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! >> >> The email you received is a Nigerian Scam. Someone was able to hack into Jerry's computer and get all his contact list! You happened to be one of his contacts. The same sort of Scam happened to me last October. Fortunately for me, I didn't have any bank account information in my computer. I have a MAC and so I went to the Apple Genius Bar and they checked my computer for any viruses. Fortunately, it is very difficult to get into a MAC. This has been on the news in Idaho and various other states. DO NOT RESPOND to any emails that want any personal information!!! You need to clean-up your computer from any viruses. >> >> >> >> Akemi Allison BS, HT (ASCP) HTL >> Director >> Phoenix Lab Consulting >> Tele: 408.335.9994 >> E-Mail: akemiat3377@yahoo.com >> >> On Aug 17, 2010, at 12:53 PM, Valantou Grover wrote: >> >> >> I am just curious, can someone check on Jerry to see if he is dead or alive, >> or if someone stole his phone/laptop to get this message to me. I remember >> the email address from Florida Society for Histotechnology, I am wondering >> if this is real or if someone is assuming his identity overseas, foul play, >> etc. >> >> >> >> Valantou Grover, Ph.D. >> >> HT/HTL(ASCP), PA, MBA >> >> Biosciences Product Line Manager >> >> Polysciences, Inc. >> >> 400 Valley Road >> >> Warrington, PA 18976 >> >> Fax: 1-800-343-3291 >> >> Phone number: 1-800-523-2575 X7418 >> >> Direct:1-215-488-7418 >> >> Cell phone: 1-215-409-8327 >> >> **************************************************************************** >> ************** >> >> * Visit us at the 36th Annual National Society for Histotechnology >> Convention - September 26-28, Seattle, WA - BOOTH 126-128 >> >> **************************************************************************** >> **************** >> >> * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex >> Course in Orlando, FL at the Grand Floridian Resort from 10/3 - 10/5. >> Please enquire at info@bangslabs.com for additional details! Phone: >> 800-387-0672, 317-570-7020. >> >> **************************************************************************** >> ***************** >> >> _____ >> >> From: JERRY SANTIAGO [mailto:santij1@bellsouth.net] >> Sent: Tuesday, August 17, 2010 1:53 PM >> To: undisclosed recipients: >> Subject: NEEDS URGENT HELP !!! >> >> >> >> >> >> Help! I'm sorry to be emailing you like this, but I am in need of some >> urgent assistance. As you have know, I've been traveling through Europe. >> But last night in Scotland, my worst fears came true. As we were headed back >> to the hotel, we were robbed but a crazy man with a knife. He took our bags, >> wallets, and phones. We have no credit cards or cash.Thankfully, we had left >> our passports in the safe at the hotel. We need to get back home, but we >> have no way to get to our flight in Germany that leaves for home next week. >> >> Right now our only option is to fly home from here. We need some money so we >> can purchase tickets to get to JFK, and then fly home from there. As soon as >> we get back home, I can repay you, I just need to get some cash to get >> there. If you can help, send me an email back, we have internet access from >> the lobby in the hotel. I'm sorry to bother you, I just didn't know how else >> to contact everyone quickly Thanks! >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Margaret.Perry <@t> sdstate.edu Tue Aug 17 16:06:19 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Aug 17 16:06:25 2010 Subject: [Histonet] muscle stain Message-ID: I am posting this for a friend. . They would like to stain muscle. The eosin and hematoxylin stain gives us too much information since it stains the nucleus separately from the cytoplasm and confuses the image analysis software. What we need is the measurement of the cell diameter only, a simpler stain. Thanks for any info you can give me. Deon Simon Any help you can give her on how to measure or a stain will be very much appreciated. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From MSHERWOOD <@t> PARTNERS.ORG Tue Aug 17 16:18:18 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Aug 17 16:17:43 2010 Subject: [Histonet] muscle stain In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24583@PHSXMB30.partners.org> Masson Trichrome stains collagen and muscle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Tuesday, August 17, 2010 5:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] muscle stain I am posting this for a friend. . They would like to stain muscle. The eosin and hematoxylin stain gives us too much information since it stains the nucleus separately from the cytoplasm and confuses the image analysis software. What we need is the measurement of the cell diameter only, a simpler stain. Thanks for any info you can give me. Deon Simon Any help you can give her on how to measure or a stain will be very much appreciated. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From patpxs <@t> gmail.com Tue Aug 17 18:25:55 2010 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Tue Aug 17 18:26:01 2010 Subject: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! In-Reply-To: References: <67097D5756B74E3080DAC19A958FC62C@USWARD13ZFB71> Message-ID: A Co-worker of mine received the same e-mail from his priest. It is a scam, someone hacked into the priest's e-mail and several people sent the poor, stranded father money ($1800 via Western Union to Scotland). My friend contacted his parish and found out that the priest was at home and didn't know he was hacked. But he was getting ready to send the money, good thing he was a little wary. This happened in May, so the scam has been out there for a little while. Be very careful. Paula :-) On Tue, Aug 17, 2010 at 4:37 PM, Akemi Allison wrote: > I am rethinking this email since I read it over again. The scam email I > received asked for money ($1,800) to be sent to England via Western Union. > I am not sure about this since this supposed Jerry is asking for just a > response email. I would still be very cautious! > > The Idaho news said the lady received a "yahoo request" (same as mine) > asking for email address, password, country of origin, mothers maiden name, > ect. Once the perpetrator had the information, they immediately extracted > all her information and cleaned her bank accounts out. This was a business > computer account and it shut down her business! > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > On Aug 17, 2010, at 1:20 PM, Valantou Grover wrote: > > Thanks, I deleted the message, now I am going to run a anti virus program. >> >> Valantou >> >> ****************************************************************************************** >> * Visit us at the 36th Annual National Society for Histotechnology >> Convention - September 26-28, Seattle, WA - BOOTH 126-128 >> >> ******************************************************************************************** >> * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex >> Course in Orlando, FL at the Grand Floridian Resort from 10/3 ? 10/5. >> Please enquire at info@bangslabs.com for additional details! Phone: >> 800-387-0672, 317-570-7020. >> >> ********************************************************************************************* >> From: Akemi Allison [mailto:akemiat3377@yahoo.com] >> Sent: Tuesday, August 17, 2010 4:14 PM >> To: Valantou Grover >> Cc: histonet@lists.utsouthwestern.edu >> Subject: Re: Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! >> >> The email you received is a Nigerian Scam. Someone was able to hack into >> Jerry's computer and get all his contact list! You happened to be one of >> his contacts. The same sort of Scam happened to me last October. >> Fortunately for me, I didn't have any bank account information in my >> computer. I have a MAC and so I went to the Apple Genius Bar and they >> checked my computer for any viruses. Fortunately, it is very difficult to >> get into a MAC. This has been on the news in Idaho and various other >> states. DO NOT RESPOND to any emails that want any personal information!!! >> You need to clean-up your computer from any viruses. >> >> >> >> Akemi Allison BS, HT (ASCP) HTL >> Director >> Phoenix Lab Consulting >> Tele: 408.335.9994 >> E-Mail: akemiat3377@yahoo.com >> >> On Aug 17, 2010, at 12:53 PM, Valantou Grover wrote: >> >> >> I am just curious, can someone check on Jerry to see if he is dead or >> alive, >> or if someone stole his phone/laptop to get this message to me. I >> remember >> the email address from Florida Society for Histotechnology, I am wondering >> if this is real or if someone is assuming his identity overseas, foul >> play, >> etc. >> >> >> >> Valantou Grover, Ph.D. >> >> HT/HTL(ASCP), PA, MBA >> >> Biosciences Product Line Manager >> >> Polysciences, Inc. >> >> 400 Valley Road >> >> Warrington, PA 18976 >> >> Fax: 1-800-343-3291 >> >> Phone number: 1-800-523-2575 X7418 >> >> Direct:1-215-488-7418 >> >> Cell phone: 1-215-409-8327 >> >> >> **************************************************************************** >> ************** >> >> * Visit us at the 36th Annual National Society for Histotechnology >> Convention - September 26-28, Seattle, WA - BOOTH 126-128 >> >> >> **************************************************************************** >> **************** >> >> * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex >> Course in Orlando, FL at the Grand Floridian Resort from 10/3 - 10/5. >> Please enquire at info@bangslabs.com for additional details! Phone: >> 800-387-0672, 317-570-7020. >> >> >> **************************************************************************** >> ***************** >> >> _____ >> >> From: JERRY SANTIAGO [mailto:santij1@bellsouth.net] >> Sent: Tuesday, August 17, 2010 1:53 PM >> To: undisclosed recipients: >> Subject: NEEDS URGENT HELP !!! >> >> >> >> >> >> Help! I'm sorry to be emailing you like this, but I am in need of some >> urgent assistance. As you have know, I've been traveling through Europe. >> But last night in Scotland, my worst fears came true. As we were headed >> back >> to the hotel, we were robbed but a crazy man with a knife. He took our >> bags, >> wallets, and phones. We have no credit cards or cash.Thankfully, we had >> left >> our passports in the safe at the hotel. We need to get back home, but we >> have no way to get to our flight in Germany that leaves for home next >> week. >> >> Right now our only option is to fly home from here. We need some money so >> we >> can purchase tickets to get to JFK, and then fly home from there. As soon >> as >> we get back home, I can repay you, I just need to get some cash to get >> there. If you can help, send me an email back, we have internet access >> from >> the lobby in the hotel. I'm sorry to bother you, I just didn't know how >> else >> to contact everyone quickly Thanks! >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab From wdesalvo.cac <@t> hotmail.com Tue Aug 17 19:05:25 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Aug 17 19:05:31 2010 Subject: [Histonet] Microtome alignment In-Reply-To: References: Message-ID: Since you have older microtomes, I suggest using an "alignment block" at each microtome instead of purchasing the alignment tools. The tools can be found on the web ttp://www.grale.com.au/products/view/804 , but they can be expensive (as much as $700.00 each). If you have more than one manufacturer for your microtomes, you will need to purchase one for each brand. Try using your largest embedding mold and make a blank block for each microtome. This can bee done first thing each morning. Use the block to align the chuck each morning before cutting. If you see drift throughout the day, add one or more checks during the day. Making a fresh block each day gives you a good standard and keeps the variation down. I also suggest you look at your embedding method and make sure you have a standardized procedure for all tissue types for orientation of tissue and exact placement in the mold. Embed your tissue on one plane with as little paraffin as possible on the bottom of the mold. Reducing variation at embedding will greatly assist you in reducing the amount of "facing" required to start producing sections and also reduce the need to align the chuck to the block/tissue. William DeSalvo, B.S., HTL(ASCP) Chair, NSH QCC Prodcution Manager, Sonora Quest Laboratories > From: Sharon.Davis-Devine@carle.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 17 Aug 2010 14:16:26 -0500 > Subject: [Histonet] Microtome alignment > > We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. > > Sharon Davis-Devine, CT (ASCP) > Cytology-Histology Supervisor > Carle Foundation Hospital > Laboratory and Pathology Services > 611 West Park Street > Urbana, Illinois 61801 > 217-383-3572 > sharon.davis-devine@carle.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Tue Aug 17 20:24:24 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue Aug 17 20:25:06 2010 Subject: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! In-Reply-To: References: <67097D5756B74E3080DAC19A958FC62C@USWARD13ZFB71> Message-ID: <969E3DBF-FF1B-4E62-8B1F-C6DAB108E927@yahoo.com> Sorry Histonetters, but this needs to be cleared up! OK, Jerry Santiago, are you alive and well in FL or the UK in trouble, or is this another ridiculous scam???? I know how well connected you are, so one of your colleagues should know....... Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Aug 17, 2010, at 1:47 PM, Eric Hill wrote: > Hi, > My wife got scammed with something almost the same a month ago, > less detailed message in hers though! They got into her gmail and > deleted all her contacts and tried to delete all her most recent > emails (they forgot to clear out her trash though, so she got most > back). > Don't fall for it! > -Eric > > Research Tech 1 > Program in Memrane Biology > Mass Genreal Hospital > > On Aug 17, 2010, at 4:37 PM, Akemi Allison wrote: > >> I am rethinking this email since I read it over again. The scam >> email I received asked for money ($1,800) to be sent to England >> via Western Union. I am not sure about this since this supposed >> Jerry is asking for just a response email. I would still be very >> cautious! >> >> The Idaho news said the lady received a "yahoo request" (same as >> mine) asking for email address, password, country of origin, >> mothers maiden name, ect. Once the perpetrator had the >> information, they immediately extracted all her information and >> cleaned her bank accounts out. This was a business computer >> account and it shut down her business! >> >> >> Akemi Allison BS, HT (ASCP) HTL >> Director >> Phoenix Lab Consulting >> Tele: 408.335.9994 >> E-Mail: akemiat3377@yahoo.com >> >> On Aug 17, 2010, at 1:20 PM, Valantou Grover wrote: >> >>> Thanks, I deleted the message, now I am going to run a anti virus >>> program. >>> >>> Valantou >>> ******************************************************************** >>> ********************** >>> * Visit us at the 36th Annual National Society for >>> Histotechnology Convention - September 26-28, Seattle, WA - BOOTH >>> 126-128 >>> ******************************************************************** >>> ************************ >>> * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 >>> Latex Course in Orlando, FL at the Grand Floridian Resort from >>> 10/3 ? 10/5. Please enquire at info@bangslabs.com for additional >>> details! Phone: 800-387-0672, 317-570-7020. >>> ******************************************************************** >>> ************************* >>> From: Akemi Allison [mailto:akemiat3377@yahoo.com] >>> Sent: Tuesday, August 17, 2010 4:14 PM >>> To: Valantou Grover >>> Cc: histonet@lists.utsouthwestern.edu >>> Subject: Re: Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! >>> >>> The email you received is a Nigerian Scam. Someone was able to >>> hack into Jerry's computer and get all his contact list! You >>> happened to be one of his contacts. The same sort of Scam >>> happened to me last October. Fortunately for me, I didn't have >>> any bank account information in my computer. I have a MAC and so >>> I went to the Apple Genius Bar and they checked my computer for >>> any viruses. Fortunately, it is very difficult to get into a >>> MAC. This has been on the news in Idaho and various other >>> states. DO NOT RESPOND to any emails that want any personal >>> information!!! You need to clean-up your computer from any viruses. >>> >>> >>> >>> Akemi Allison BS, HT (ASCP) HTL >>> Director >>> Phoenix Lab Consulting >>> Tele: 408.335.9994 >>> E-Mail: akemiat3377@yahoo.com >>> >>> On Aug 17, 2010, at 12:53 PM, Valantou Grover wrote: >>> >>> >>> I am just curious, can someone check on Jerry to see if he is >>> dead or alive, >>> or if someone stole his phone/laptop to get this message to me. >>> I remember >>> the email address from Florida Society for Histotechnology, I am >>> wondering >>> if this is real or if someone is assuming his identity overseas, >>> foul play, >>> etc. >>> >>> >>> >>> Valantou Grover, Ph.D. >>> >>> HT/HTL(ASCP), PA, MBA >>> >>> Biosciences Product Line Manager >>> >>> Polysciences, Inc. >>> >>> 400 Valley Road >>> >>> Warrington, PA 18976 >>> >>> Fax: 1-800-343-3291 >>> >>> Phone number: 1-800-523-2575 X7418 >>> >>> Direct:1-215-488-7418 >>> >>> Cell phone: 1-215-409-8327 >>> >>> ******************************************************************** >>> ******** >>> ************** >>> >>> * Visit us at the 36th Annual National Society for Histotechnology >>> Convention - September 26-28, Seattle, WA - BOOTH 126-128 >>> >>> ******************************************************************** >>> ******** >>> **************** >>> >>> * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 >>> Latex >>> Course in Orlando, FL at the Grand Floridian Resort from 10/3 - >>> 10/5. >>> Please enquire at info@bangslabs.com for additional details! Phone: >>> 800-387-0672, 317-570-7020. >>> >>> ******************************************************************** >>> ******** >>> ***************** >>> >>> _____ >>> >>> From: JERRY SANTIAGO [mailto:santij1@bellsouth.net] >>> Sent: Tuesday, August 17, 2010 1:53 PM >>> To: undisclosed recipients: >>> Subject: NEEDS URGENT HELP !!! >>> >>> >>> >>> >>> >>> Help! I'm sorry to be emailing you like this, but I am in need of >>> some >>> urgent assistance. As you have know, I've been traveling through >>> Europe. >>> But last night in Scotland, my worst fears came true. As we were >>> headed back >>> to the hotel, we were robbed but a crazy man with a knife. He >>> took our bags, >>> wallets, and phones. We have no credit cards or cash.Thankfully, >>> we had left >>> our passports in the safe at the hotel. We need to get back home, >>> but we >>> have no way to get to our flight in Germany that leaves for home >>> next week. >>> >>> Right now our only option is to fly home from here. We need some >>> money so we >>> can purchase tickets to get to JFK, and then fly home from there. >>> As soon as >>> we get back home, I can repay you, I just need to get some cash >>> to get >>> there. If you can help, send me an email back, we have internet >>> access from >>> the lobby in the hotel. I'm sorry to bother you, I just didn't >>> know how else >>> to contact everyone quickly Thanks! >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > The information in this e-mail is intended only for the person to > whom it is > addressed. If you believe this e-mail was sent to you in error and > the e-mail > contains patient information, please contact the Partners > Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to > you in error > but does not contain patient information, please contact the sender > and properly > dispose of the e-mail. > From kgrobert <@t> rci.rutgers.edu Tue Aug 17 20:42:59 2010 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Tue Aug 17 20:43:04 2010 Subject: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! In-Reply-To: <969E3DBF-FF1B-4E62-8B1F-C6DAB108E927@yahoo.com> References: <67097D5756B74E3080DAC19A958FC62C@USWARD13ZFB71> <969E3DBF-FF1B-4E62-8B1F-C6DAB108E927@yahoo.com> Message-ID: <7bee8c220630b60f8ee8952fdcf75d0d.squirrel@webmail.rci.rutgers.edu> This looks similar to a scam documented on Snopes.com, a website that clears up rumors, scams and urban legends: http://www.snopes.com/fraud/distress/family.asp Check out the second story in this category...very similar, no? Kathleen > Sorry Histonetters, but this needs to be cleared up! OK, Jerry > Santiago, are you alive and well in FL or the UK in trouble, or is > this another ridiculous scam???? I know how well connected you are, > so one of your colleagues should know....... > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > On Aug 17, 2010, at 1:47 PM, Eric Hill wrote: > >> Hi, >> My wife got scammed with something almost the same a month ago, >> less detailed message in hers though! They got into her gmail and >> deleted all her contacts and tried to delete all her most recent >> emails (they forgot to clear out her trash though, so she got most >> back). >> Don't fall for it! >> -Eric >> >> Research Tech 1 >> Program in Memrane Biology >> Mass Genreal Hospital >> >> On Aug 17, 2010, at 4:37 PM, Akemi Allison wrote: >> >>> I am rethinking this email since I read it over again. The scam >>> email I received asked for money ($1,800) to be sent to England >>> via Western Union. I am not sure about this since this supposed >>> Jerry is asking for just a response email. I would still be very >>> cautious! >>> >>> The Idaho news said the lady received a "yahoo request" (same as >>> mine) asking for email address, password, country of origin, >>> mothers maiden name, ect. Once the perpetrator had the >>> information, they immediately extracted all her information and >>> cleaned her bank accounts out. This was a business computer >>> account and it shut down her business! >>> >>> >>> Akemi Allison BS, HT (ASCP) HTL >>> Director >>> Phoenix Lab Consulting >>> Tele: 408.335.9994 >>> E-Mail: akemiat3377@yahoo.com >>> >>> On Aug 17, 2010, at 1:20 PM, Valantou Grover wrote: >>> >>>> Thanks, I deleted the message, now I am going to run a anti virus >>>> program. >>>> >>>> Valantou >>>> ******************************************************************** >>>> ********************** >>>> * Visit us at the 36th Annual National Society for >>>> Histotechnology Convention - September 26-28, Seattle, WA - BOOTH >>>> 126-128 >>>> ******************************************************************** >>>> ************************ >>>> * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 >>>> Latex Course in Orlando, FL at the Grand Floridian Resort from >>>> 10/3 ? 10/5. Please enquire at info@bangslabs.com for additional >>>> details! Phone: 800-387-0672, 317-570-7020. >>>> ******************************************************************** >>>> ************************* >>>> From: Akemi Allison [mailto:akemiat3377@yahoo.com] >>>> Sent: Tuesday, August 17, 2010 4:14 PM >>>> To: Valantou Grover >>>> Cc: histonet@lists.utsouthwestern.edu >>>> Subject: Re: Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! >>>> >>>> The email you received is a Nigerian Scam. Someone was able to >>>> hack into Jerry's computer and get all his contact list! You >>>> happened to be one of his contacts. The same sort of Scam >>>> happened to me last October. Fortunately for me, I didn't have >>>> any bank account information in my computer. I have a MAC and so >>>> I went to the Apple Genius Bar and they checked my computer for >>>> any viruses. Fortunately, it is very difficult to get into a >>>> MAC. This has been on the news in Idaho and various other >>>> states. DO NOT RESPOND to any emails that want any personal >>>> information!!! You need to clean-up your computer from any viruses. >>>> >>>> >>>> >>>> Akemi Allison BS, HT (ASCP) HTL >>>> Director >>>> Phoenix Lab Consulting >>>> Tele: 408.335.9994 >>>> E-Mail: akemiat3377@yahoo.com >>>> >>>> On Aug 17, 2010, at 12:53 PM, Valantou Grover wrote: >>>> >>>> >>>> I am just curious, can someone check on Jerry to see if he is >>>> dead or alive, >>>> or if someone stole his phone/laptop to get this message to me. >>>> I remember >>>> the email address from Florida Society for Histotechnology, I am >>>> wondering >>>> if this is real or if someone is assuming his identity overseas, >>>> foul play, >>>> etc. >>>> >>>> >>>> >>>> Valantou Grover, Ph.D. >>>> >>>> HT/HTL(ASCP), PA, MBA >>>> >>>> Biosciences Product Line Manager >>>> >>>> Polysciences, Inc. >>>> >>>> 400 Valley Road >>>> >>>> Warrington, PA 18976 >>>> >>>> Fax: 1-800-343-3291 >>>> >>>> Phone number: 1-800-523-2575 X7418 >>>> >>>> Direct:1-215-488-7418 >>>> >>>> Cell phone: 1-215-409-8327 >>>> >>>> ******************************************************************** >>>> ******** >>>> ************** >>>> >>>> * Visit us at the 36th Annual National Society for Histotechnology >>>> Convention - September 26-28, Seattle, WA - BOOTH 126-128 >>>> >>>> ******************************************************************** >>>> ******** >>>> **************** >>>> >>>> * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 >>>> Latex >>>> Course in Orlando, FL at the Grand Floridian Resort from 10/3 - >>>> 10/5. >>>> Please enquire at info@bangslabs.com for additional details! Phone: >>>> 800-387-0672, 317-570-7020. >>>> >>>> ******************************************************************** >>>> ******** >>>> ***************** >>>> >>>> _____ >>>> >>>> From: JERRY SANTIAGO [mailto:santij1@bellsouth.net] >>>> Sent: Tuesday, August 17, 2010 1:53 PM >>>> To: undisclosed recipients: >>>> Subject: NEEDS URGENT HELP !!! >>>> >>>> >>>> >>>> >>>> >>>> Help! I'm sorry to be emailing you like this, but I am in need of >>>> some >>>> urgent assistance. As you have know, I've been traveling through >>>> Europe. >>>> But last night in Scotland, my worst fears came true. As we were >>>> headed back >>>> to the hotel, we were robbed but a crazy man with a knife. He >>>> took our bags, >>>> wallets, and phones. We have no credit cards or cash.Thankfully, >>>> we had left >>>> our passports in the safe at the hotel. We need to get back home, >>>> but we >>>> have no way to get to our flight in Germany that leaves for home >>>> next week. >>>> >>>> Right now our only option is to fly home from here. We need some >>>> money so we >>>> can purchase tickets to get to JFK, and then fly home from there. >>>> As soon as >>>> we get back home, I can repay you, I just need to get some cash >>>> to get >>>> there. If you can help, send me an email back, we have internet >>>> access from >>>> the lobby in the hotel. I'm sorry to bother you, I just didn't >>>> know how else >>>> to contact everyone quickly Thanks! >>>> >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> The information in this e-mail is intended only for the person to >> whom it is >> addressed. If you believe this e-mail was sent to you in error and >> the e-mail >> contains patient information, please contact the Partners >> Compliance HelpLine at >> http://www.partners.org/complianceline . If the e-mail was sent to >> you in error >> but does not contain patient information, please contact the sender >> and properly >> dispose of the e-mail. >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 From jsantiago <@t> bellsouth.net Tue Aug 17 22:52:22 2010 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Tue Aug 17 22:52:26 2010 Subject: [Histonet] I Have been scammed Message-ID: <286916.62275.qm@web180820.mail.gq1.yahoo.com> Histonetters, Thanks for all of your concerns and calls. I was made aware of the Europe scam and have contacted the authorities and they are doing what they suppose to do. I am still in Florida and have not left the country for any reason. My e-mail company states that this was stolen from facebook and gained access to my email account cleaning out all of my contacts and my access to my e-mail account as well as facebook. It will be fine and I apologize for anyone that got caught in this scam. Jerry Santiago Shands Jacksonville Jacksonville, FL 904-244-6149 From bstephen <@t> fastmail.fm Wed Aug 18 01:57:52 2010 From: bstephen <@t> fastmail.fm (Birgitta Stephenson) Date: Wed Aug 18 01:57:59 2010 Subject: [Histonet] muscle stain In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24583@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24583@PHSXMB30.partners.org> Message-ID: <1282114672.13120.1390474803@webmail.messagingengine.com> Toluidine blue stains striated muscle nicely. Regards Birgitta Stephenson University of Queensland Microscopy Research Lab On Tue, 17 Aug 2010 17:18:18 -0400, "Sherwood, Margaret " said: > Masson Trichrome stains collagen and muscle. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, > Margaret > Sent: Tuesday, August 17, 2010 5:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] muscle stain > > I am posting this for a friend. . They would like to stain muscle. > The eosin and hematoxylin stain gives us too much information since it > stains > the nucleus separately from the cytoplasm and confuses the image analysis > software. What we need is the measurement of the cell diameter only, a > simpler > stain. Thanks for any info you can give me. Deon Simon > Any help you can give her on how to measure or a stain will be very much > appreciated. > > Margaret Perry HT(ASCP) > Dept of Veterinary and Biomedical services > Box 2175 > South Dakota State University > Brookings SD 57007 > 605-688-5638 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it > is > addressed. If you believe this e-mail was sent to you in error and the > e-mail > contains patient information, please contact the Partners Compliance > HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in > error > but does not contain patient information, please contact the sender and > properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Birgitta Stephenson bstephen@fastmail.fm -- http://www.fastmail.fm - A no graphics, no pop-ups email service From joelleweaver <@t> hotmail.com Wed Aug 18 04:19:55 2010 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 18 04:20:00 2010 Subject: [Histonet] Microtome alignment In-Reply-To: References: Message-ID: This is a learned histology skill, but there are some devices which can be used to align different microtomes available, and you can test your adjustments with "blank" blocks. Also newer microtomes have a device on the block holder that will align the position in the X-Y axis in the "zero" position. Joelle Weaver > From: Sharon.Davis-Devine@carle.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 17 Aug 2010 14:16:26 -0500 > Subject: [Histonet] Microtome alignment > > We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. > > Sharon Davis-Devine, CT (ASCP) > Cytology-Histology Supervisor > Carle Foundation Hospital > Laboratory and Pathology Services > 611 West Park Street > Urbana, Illinois 61801 > 217-383-3572 > sharon.davis-devine@carle.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Wed Aug 18 06:00:23 2010 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Wed Aug 18 06:00:30 2010 Subject: [Histonet] Microtome alignment In-Reply-To: References: Message-ID: A while back I remember someone suggestion something like a right angle device that carpenters use. It's basically just a piece of metal that is a right angle triangle that you put up against the chuck and on the knife mount. Then you align the chuck so it is a a right angle to the knife mount. It looks like this: l\ l \ l \ This is my best attempt at computer drawing. l __ \ I don't thing they cost very much, much less that $700. Paula :-) On Tue, Aug 17, 2010 at 8:05 PM, WILLIAM DESALVO wrote: > > Since you have older microtomes, I suggest using an "alignment block" at > each microtome instead of purchasing the alignment tools. The tools can be > found on the web ttp://www.grale.com.au/products/view/804 , but they can > be expensive (as much as $700.00 each). If you have more than one > manufacturer for your microtomes, you will need to purchase one for each > brand. > > Try using your largest embedding mold and make a blank block for each > microtome. This can bee done first thing each morning. Use the block to > align the chuck each morning before cutting. If you see drift throughout the > day, add one or more checks during the day. Making a fresh block each day > gives you a good standard and keeps the variation down. > > I also suggest you look at your embedding method and make sure you have a > standardized procedure for all tissue types for orientation of tissue and > exact placement in the mold. Embed your tissue on one plane with as little > paraffin as possible on the bottom of the mold. Reducing variation at > embedding will greatly assist you in reducing the amount of "facing" > required to start producing sections and also reduce the need to align the > chuck to the block/tissue. > > William DeSalvo, B.S., HTL(ASCP) > Chair, NSH QCC > Prodcution Manager, Sonora Quest Laboratories > > > > > > From: Sharon.Davis-Devine@carle.com > > To: histonet@lists.utsouthwestern.edu > > Date: Tue, 17 Aug 2010 14:16:26 -0500 > > Subject: [Histonet] Microtome alignment > > > > We are having a continuing issue of too much tissue being cut off when > facing off a block for recuts. We have tried a couple of different methods > for aligning our microtomes without much success. Does anyone out there have > any advice on how to properly align them and what tool to use? Also, how > often do you perform this re-alignment? The majority of our microtomes are > older so more wear and tear and things move out of place more often. Any > help or suggestions will be greatly appreciated. Thanks. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology-Histology Supervisor > > Carle Foundation Hospital > > Laboratory and Pathology Services > > 611 West Park Street > > Urbana, Illinois 61801 > > 217-383-3572 > > sharon.davis-devine@carle.com > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab From joelleweaver <@t> hotmail.com Wed Aug 18 06:34:34 2010 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 18 06:34:39 2010 Subject: [Histonet] Microtome alignment In-Reply-To: References: , , Message-ID: Yes, these used to be available from Newcomer supply, but I think they are now de-funked. Try a google search perhaps? Joelle > Date: Wed, 18 Aug 2010 07:00:23 -0400 > From: patpxs@gmail.com > To: wdesalvo.cac@hotmail.com > Subject: Re: [Histonet] Microtome alignment > CC: histonet@lists.utsouthwestern.edu > > A while back I remember someone suggestion something like a right angle > device that carpenters use. It's basically just a piece of metal that is a > right angle triangle that you put up against the chuck and on the knife > mount. Then you align the chuck so it is a a right angle to the knife > mount. > > It looks like this: l\ > l \ > l \ This is my best attempt at computer > drawing. > l __ \ > > I don't thing they cost very much, much less that $700. > > > Paula :-) > > > > On Tue, Aug 17, 2010 at 8:05 PM, WILLIAM DESALVO > wrote: > > > > > Since you have older microtomes, I suggest using an "alignment block" at > > each microtome instead of purchasing the alignment tools. The tools can be > > found on the web ttp://www.grale.com.au/products/view/804 , but they can > > be expensive (as much as $700.00 each). If you have more than one > > manufacturer for your microtomes, you will need to purchase one for each > > brand. > > > > Try using your largest embedding mold and make a blank block for each > > microtome. This can bee done first thing each morning. Use the block to > > align the chuck each morning before cutting. If you see drift throughout the > > day, add one or more checks during the day. Making a fresh block each day > > gives you a good standard and keeps the variation down. > > > > I also suggest you look at your embedding method and make sure you have a > > standardized procedure for all tissue types for orientation of tissue and > > exact placement in the mold. Embed your tissue on one plane with as little > > paraffin as possible on the bottom of the mold. Reducing variation at > > embedding will greatly assist you in reducing the amount of "facing" > > required to start producing sections and also reduce the need to align the > > chuck to the block/tissue. > > > > William DeSalvo, B.S., HTL(ASCP) > > Chair, NSH QCC > > Prodcution Manager, Sonora Quest Laboratories > > > > > > > > > > > From: Sharon.Davis-Devine@carle.com > > > To: histonet@lists.utsouthwestern.edu > > > Date: Tue, 17 Aug 2010 14:16:26 -0500 > > > Subject: [Histonet] Microtome alignment > > > > > > We are having a continuing issue of too much tissue being cut off when > > facing off a block for recuts. We have tried a couple of different methods > > for aligning our microtomes without much success. Does anyone out there have > > any advice on how to properly align them and what tool to use? Also, how > > often do you perform this re-alignment? The majority of our microtomes are > > older so more wear and tear and things move out of place more often. Any > > help or suggestions will be greatly appreciated. Thanks. > > > > > > Sharon Davis-Devine, CT (ASCP) > > > Cytology-Histology Supervisor > > > Carle Foundation Hospital > > > Laboratory and Pathology Services > > > 611 West Park Street > > > Urbana, Illinois 61801 > > > 217-383-3572 > > > sharon.davis-devine@carle.com > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Paula Sicurello > 6 of 6 > Duke Healthcare System EM Lab > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amario3 <@t> uic.edu Wed Aug 18 08:18:45 2010 From: amario3 <@t> uic.edu (Andrea Marion) Date: Wed Aug 18 08:18:52 2010 Subject: [Histonet] Re: muscle stain In-Reply-To: <20100818065838.CDE0C1A486C3@barracuda.uic.edu> References: <20100818065838.CDE0C1A486C3@barracuda.uic.edu> Message-ID: There is an immunofluorescence technique that uses wheat germ agglutinin (WGA) conjugated to a fluorophore (usually FITC). WGA binds only to the cell periphery, leaving you with beautiful traces of the outside of each cell. The cell area is then simple to analyze with ImageJ or any other software program. It's often used to measure cardiomyocyte size in hypertrophy studies. I can provide a protocol if requested. If you have access to this article, there is a detailed description of the technique and great images: Regional changes in myocyte structure in model of canine right atrial hypertrophy. Am J Physiol Heart Circ Physiol 267: H1279-H1287, 1994. P. C. Dolber, R. P. Bauman, J. C. Rembert and J. C. Greenfield Jr. If not, here is a freely accessible publication. Figure 9, panel B shows what the result looks like. Cardiomyocyte GATA4 functions as a stress-responsive regulator of angiogenesis in the murine heart. J Clin Invest. 2007; 117(11):3198. http://www.jci.org/articles/view/32573/figure/9 Andrea Marion Graduate Student University of Illinois - Chicago amario3 uic edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] muscle stain > > I am posting this for a friend. . They would like to stain muscle. > The eosin and hematoxylin stain gives us too much information since it > stains > the nucleus separately from the cytoplasm and confuses the image analysis > software. What we need is the measurement of the cell diameter only, a > simpler > stain. Thanks for any info you can give me. Deon Simon > Any help you can give her on how to measure or a stain will be very much > appreciated. > > Margaret Perry HT(ASCP) > Dept of Veterinary and Biomedical services > Box 2175 > South Dakota State University > Brookings SD 57007 > 605-688-5638 > From shive003 <@t> umn.edu Wed Aug 18 08:30:00 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Aug 18 08:30:05 2010 Subject: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! References: <67097D5756B74E3080DAC19A958FC62C@USWARD13ZFB71> <969E3DBF-FF1B-4E62-8B1F-C6DAB108E927@yahoo.com> Message-ID: <7CE1ACA42C5343B09891FA1AFAC9228F@auxs.umn.edu> Reminder... these 'please send money' or 'please send your bank acct information so that I can deposit money into your acct' pleas are ALWAYS scams. They have been for years. If a trusted 'friend' was truly stranded in a foreign country, they'd borrow a phone and call home. If a long lost relative died and left you money in another country, it would be a lawyer contacting you via telephone or letter, and definitely not through an email message. Best advice... don't even open these messages up. They're bogus, and could be carrying viruses, worms, etc. themselves. Being on a listserv like histonet, we're prone to receive a lot of these email fraud attempts. Ignore them. Jan Shivers ----- Original Message ----- From: "Akemi Allison" To: Cc: ; "Valantou Grover" Sent: Tuesday, August 17, 2010 8:24 PM Subject: Re: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! Sorry Histonetters, but this needs to be cleared up! OK, Jerry Santiago, are you alive and well in FL or the UK in trouble, or is this another ridiculous scam???? I know how well connected you are, so one of your colleagues should know....... Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Aug 17, 2010, at 1:47 PM, Eric Hill wrote: > Hi, > My wife got scammed with something almost the same a month ago, less > detailed message in hers though! They got into her gmail and deleted all > her contacts and tried to delete all her most recent emails (they forgot > to clear out her trash though, so she got most back). > Don't fall for it! > -Eric > > Research Tech 1 > Program in Memrane Biology > Mass Genreal Hospital > > On Aug 17, 2010, at 4:37 PM, Akemi Allison wrote: > >> I am rethinking this email since I read it over again. The scam email I >> received asked for money ($1,800) to be sent to England via Western >> Union. I am not sure about this since this supposed Jerry is asking for >> just a response email. I would still be very cautious! >> >> The Idaho news said the lady received a "yahoo request" (same as mine) >> asking for email address, password, country of origin, mothers maiden >> name, ect. Once the perpetrator had the information, they immediately >> extracted all her information and cleaned her bank accounts out. This >> was a business computer account and it shut down her business! >> >> >> Akemi Allison BS, HT (ASCP) HTL >> Director >> Phoenix Lab Consulting >> Tele: 408.335.9994 >> E-Mail: akemiat3377@yahoo.com >> >> On Aug 17, 2010, at 1:20 PM, Valantou Grover wrote: >> >>> Thanks, I deleted the message, now I am going to run a anti virus >>> program. >>> >>> Valantou >>> ******************************************************************** >>> ********************** >>> * Visit us at the 36th Annual National Society for Histotechnology >>> Convention - September 26-28, Seattle, WA - BOOTH 126-128 >>> ******************************************************************** >>> ************************ >>> * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex >>> Course in Orlando, FL at the Grand Floridian Resort from 10/3 ? 10/5. >>> Please enquire at info@bangslabs.com for additional details! Phone: >>> 800-387-0672, 317-570-7020. >>> ******************************************************************** >>> ************************* >>> From: Akemi Allison [mailto:akemiat3377@yahoo.com] >>> Sent: Tuesday, August 17, 2010 4:14 PM >>> To: Valantou Grover >>> Cc: histonet@lists.utsouthwestern.edu >>> Subject: Re: Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! >>> >>> The email you received is a Nigerian Scam. Someone was able to hack >>> into Jerry's computer and get all his contact list! You happened to be >>> one of his contacts. The same sort of Scam happened to me last >>> October. Fortunately for me, I didn't have any bank account >>> information in my computer. I have a MAC and so I went to the Apple >>> Genius Bar and they checked my computer for any viruses. Fortunately, >>> it is very difficult to get into a MAC. This has been on the news in >>> Idaho and various other states. DO NOT RESPOND to any emails that want >>> any personal information!!! You need to clean-up your computer from >>> any viruses. >>> >>> >>> >>> Akemi Allison BS, HT (ASCP) HTL >>> Director >>> Phoenix Lab Consulting >>> Tele: 408.335.9994 >>> E-Mail: akemiat3377@yahoo.com >>> >>> On Aug 17, 2010, at 12:53 PM, Valantou Grover wrote: >>> >>> >>> I am just curious, can someone check on Jerry to see if he is dead or >>> alive, >>> or if someone stole his phone/laptop to get this message to me. I >>> remember >>> the email address from Florida Society for Histotechnology, I am >>> wondering >>> if this is real or if someone is assuming his identity overseas, foul >>> play, >>> etc. >>> >>> >>> >>> Valantou Grover, Ph.D. >>> >>> HT/HTL(ASCP), PA, MBA >>> >>> Biosciences Product Line Manager >>> >>> Polysciences, Inc. >>> >>> 400 Valley Road >>> >>> Warrington, PA 18976 >>> >>> Fax: 1-800-343-3291 >>> >>> Phone number: 1-800-523-2575 X7418 >>> >>> Direct:1-215-488-7418 >>> >>> Cell phone: 1-215-409-8327 >>> >>> ******************************************************************** >>> ******** >>> ************** >>> >>> * Visit us at the 36th Annual National Society for Histotechnology >>> Convention - September 26-28, Seattle, WA - BOOTH 126-128 >>> >>> ******************************************************************** >>> ******** >>> **************** >>> >>> * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex >>> Course in Orlando, FL at the Grand Floridian Resort from 10/3 - 10/5. >>> Please enquire at info@bangslabs.com for additional details! Phone: >>> 800-387-0672, 317-570-7020. >>> >>> ******************************************************************** >>> ******** >>> ***************** >>> >>> _____ >>> >>> From: JERRY SANTIAGO [mailto:santij1@bellsouth.net] >>> Sent: Tuesday, August 17, 2010 1:53 PM >>> To: undisclosed recipients: >>> Subject: NEEDS URGENT HELP !!! >>> >>> >>> >>> >>> >>> Help! I'm sorry to be emailing you like this, but I am in need of some >>> urgent assistance. As you have know, I've been traveling through >>> Europe. >>> But last night in Scotland, my worst fears came true. As we were headed >>> back >>> to the hotel, we were robbed but a crazy man with a knife. He took our >>> bags, >>> wallets, and phones. We have no credit cards or cash.Thankfully, we had >>> left >>> our passports in the safe at the hotel. We need to get back home, but >>> we >>> have no way to get to our flight in Germany that leaves for home next >>> week. >>> >>> Right now our only option is to fly home from here. We need some money >>> so we >>> can purchase tickets to get to JFK, and then fly home from there. As >>> soon as >>> we get back home, I can repay you, I just need to get some cash to get >>> there. If you can help, send me an email back, we have internet access >>> from >>> the lobby in the hotel. I'm sorry to bother you, I just didn't know how >>> else >>> to contact everyone quickly Thanks! >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > The information in this e-mail is intended only for the person to whom it > is > addressed. If you believe this e-mail was sent to you in error and the > e-mail > contains patient information, please contact the Partners Compliance > HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in > error > but does not contain patient information, please contact the sender and > properly > dispose of the e-mail. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Wed Aug 18 08:37:07 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Aug 18 08:37:11 2010 Subject: [Histonet] thymidine kinase 1 Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D70CB70B@usctmx1176.merck.com> Does anyone have experience with the rabbit monoclonal anti-thymidine kinase 1 (clone EPR3193) on FFPE tissues? i.e. antigen retrieval necessary or not? Thanks as always, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From leiker <@t> buffalo.edu Wed Aug 18 08:39:32 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Aug 18 08:39:39 2010 Subject: [Histonet] Re: muscle stain In-Reply-To: References: <20100818065838.CDE0C1A486C3@barracuda.uic.edu> Message-ID: <6E3D1638B2F4B3DFA1669C0C@CDYwxp1931.ad.med.buffalo.edu> Andrea - Wow this looks great. We do a lot of cardiomyocyte staining and studying and this could be helpful. Thanks for sharing! Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. --On Wednesday, August 18, 2010 8:18 AM -0500 Andrea Marion wrote: > There is an immunofluorescence technique that uses wheat germ agglutinin > (WGA) conjugated to a fluorophore (usually FITC). WGA binds only to the > cell periphery, leaving you with beautiful traces of the outside of each > cell. The cell area is then simple to analyze with ImageJ or any other > software program. It's often used to measure cardiomyocyte size in > hypertrophy studies. I can provide a protocol if requested. > > If you have access to this article, there is a detailed description of the > technique and great images: Regional changes in myocyte structure in model > of canine right atrial hypertrophy. Am J Physiol Heart Circ Physiol 267: > H1279-H1287, 1994. P. C. Dolber, R. P. Bauman, J. C. Rembert and J. C. > Greenfield Jr. > > If not, here is a freely accessible publication. Figure 9, panel B shows > what the result looks like. Cardiomyocyte GATA4 functions as a > stress-responsive regulator of angiogenesis in the murine heart. J Clin > Invest. 2007; 117(11):3198. > http://www.jci.org/articles/view/32573/figure/9 > > Andrea Marion > Graduate Student > University of Illinois - Chicago > amario3 uic edu > >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] muscle stain >> >> I am posting this for a friend. . They would like to stain muscle. >> The eosin and hematoxylin stain gives us too much information since it >> stains >> the nucleus separately from the cytoplasm and confuses the image analysis >> software. What we need is the measurement of the cell diameter only, a >> simpler >> stain. Thanks for any info you can give me. Deon Simon >> Any help you can give her on how to measure or a stain will be very much >> appreciated. >> >> Margaret Perry HT(ASCP) >> Dept of Veterinary and Biomedical services >> Box 2175 >> South Dakota State University >> Brookings SD 57007 >> 605-688-5638 >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Reuel.Cornelia <@t> tsrh.org Wed Aug 18 08:55:48 2010 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Wed Aug 18 08:56:06 2010 Subject: [Histonet] Test for cell viability in paraffin section? Message-ID: <4C6BA014020000C500080345@mail.TSRH.ORG> Would you know a techniqe to confirm cell viability in paraffin embedded tissue? Propidium and methylene blue staining are used in cell culturing to detect cell viability. Is there similar technique for detecting cell viability in tissue? This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue? Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From rjbuesa <@t> yahoo.com Wed Aug 18 09:06:39 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 18 09:06:43 2010 Subject: [Histonet] Test for cell viability in paraffin section? In-Reply-To: <4C6BA014020000C500080345@mail.TSRH.ORG> Message-ID: <853336.627.qm@web65711.mail.ac4.yahoo.com> Cornelia: After a "deadly" fixation, followed by a complete dehydration, clearing and paraffin wax infiltration, the only potentially surviving "entities" are prions. No cell will be viable after this treatment and if you get some positive results of viability with what ever test you use, I caution to believe those results. Your research director evidently is absolutely ignorant about tissue processing! Ren? J. --- On Wed, 8/18/10, Reuel Cornelia wrote: From: Reuel Cornelia Subject: [Histonet] Test for cell viability in paraffin section? To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 18, 2010, 9:55 AM Would you know a? techniqe to confirm cell viability in paraffin embedded tissue?? Propidium and methylene blue staining are used in cell culturing to detect cell viability.? Is there similar technique for detecting cell viability in tissue? This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue? Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laura.Miller <@t> leica-microsystems.com Wed Aug 18 09:07:16 2010 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Wed Aug 18 09:07:25 2010 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 08/18/2010 and will not return until 08/20/2010. I will be conducting training the rest of the week. I will respond to your message as quickly as possibe. Thanks! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From alyssa <@t> alliedsearchpartners.com Wed Aug 18 09:11:25 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Aug 18 09:11:30 2010 Subject: [Histonet] Histotech Needed For Vacation Coverage In GA-1 Week Message-ID: MPath Search Partners has been retained to search for a Histotechnologist/Histotechnician who is available for a vacation coverage shift in Marietta, GA. Position Title: Histotechnologist/Histotechnician Shift: September 6th-10th, 2010. That?s Monday-Friday 6-8 hour days with lunch Location & Environment: Physicians Office Laboratory A practice filled with state-of-the-art- equipment, and cutting edge technology Requirements: Ability to work with no supervision HT/HTL ASCP preferred Experience working with Dermatology tissue Other: This position is just a temporary position, allowing the current histotech to take a vacation. To apply: Please send information to Alyssa@alliedsearchpartners.com 1. Resume 2. Expected Salary 3. Hours you are available to work -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From TMcNemar <@t> lmhealth.org Wed Aug 18 09:11:42 2010 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Aug 18 09:11:41 2010 Subject: [Histonet] Microtome alignment In-Reply-To: Message-ID: I assume you are referring to a small carpenter's square. That will only allow you to square up the chuck from top to bottom. It would not work for left and right adjustments. Marketlab sells an alignment tool that they claim will work for multiple microtomes. I have not tried it though. It lists for $775. I have considered having a piece of steel welded to an old knife holder and just adjust the chuck until it is flat against the steel. Should work and won't cost $775. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Wednesday, August 18, 2010 7:00 AM To: WILLIAM DESALVO Cc: histonet Subject: Re: [Histonet] Microtome alignment A while back I remember someone suggestion something like a right angle device that carpenters use. It's basically just a piece of metal that is a right angle triangle that you put up against the chuck and on the knife mount. Then you align the chuck so it is a a right angle to the knife mount. It looks like this: l\ l \ l \ This is my best attempt at computer drawing. l __ \ I don't thing they cost very much, much less that $700. Paula :-) On Tue, Aug 17, 2010 at 8:05 PM, WILLIAM DESALVO wrote: > > Since you have older microtomes, I suggest using an "alignment block" at > each microtome instead of purchasing the alignment tools. The tools can be > found on the web ttp://www.grale.com.au/products/view/804 , but they can > be expensive (as much as $700.00 each). If you have more than one > manufacturer for your microtomes, you will need to purchase one for each > brand. > > Try using your largest embedding mold and make a blank block for each > microtome. This can bee done first thing each morning. Use the block to > align the chuck each morning before cutting. If you see drift throughout the > day, add one or more checks during the day. Making a fresh block each day > gives you a good standard and keeps the variation down. > > I also suggest you look at your embedding method and make sure you have a > standardized procedure for all tissue types for orientation of tissue and > exact placement in the mold. Embed your tissue on one plane with as little > paraffin as possible on the bottom of the mold. Reducing variation at > embedding will greatly assist you in reducing the amount of "facing" > required to start producing sections and also reduce the need to align the > chuck to the block/tissue. > > William DeSalvo, B.S., HTL(ASCP) > Chair, NSH QCC > Prodcution Manager, Sonora Quest Laboratories > > > > > > From: Sharon.Davis-Devine@carle.com > > To: histonet@lists.utsouthwestern.edu > > Date: Tue, 17 Aug 2010 14:16:26 -0500 > > Subject: [Histonet] Microtome alignment > > > > We are having a continuing issue of too much tissue being cut off when > facing off a block for recuts. We have tried a couple of different methods > for aligning our microtomes without much success. Does anyone out there have > any advice on how to properly align them and what tool to use? Also, how > often do you perform this re-alignment? The majority of our microtomes are > older so more wear and tear and things move out of place more often. Any > help or suggestions will be greatly appreciated. Thanks. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology-Histology Supervisor > > Carle Foundation Hospital > > Laboratory and Pathology Services > > 611 West Park Street > > Urbana, Illinois 61801 > > 217-383-3572 > > sharon.davis-devine@carle.com > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From annigyg <@t> gmail.com Wed Aug 18 09:11:24 2010 From: annigyg <@t> gmail.com (annigyg@gmail.com) Date: Wed Aug 18 09:12:11 2010 Subject: [Histonet] Test for cell viability in paraffin section? In-Reply-To: <4C6BA014020000C500080345@mail.TSRH.ORG> References: <4C6BA014020000C500080345@mail.TSRH.ORG> Message-ID: <776733000-1282140710-cardhu_decombobulator_blackberry.rim.net-194124245-@bda269.bisx.produk.on.blackberry> Hey Ruel Once we are done with them and they are all nicely embedded in paraffin wax...the cells are not viable...they are DEAD Annieinarabia Empower your Business with BlackBerry? and Mobile Solutions from Etisalat -----Original Message----- From: "Reuel Cornelia" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 18 Aug 2010 08:55:48 To: Subject: [Histonet] Test for cell viability in paraffin section? Would you know a techniqe to confirm cell viability in paraffin embedded tissue? Propidium and methylene blue staining are used in cell culturing to detect cell viability. Is there similar technique for detecting cell viability in tissue? This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue? Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Wed Aug 18 09:39:17 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Wed Aug 18 09:39:23 2010 Subject: [Histonet] Test for cell viability in paraffin section? In-Reply-To: <4C6BA014020000C500080345@mail.TSRH.ORG> References: <4C6BA014020000C500080345@mail.TSRH.ORG> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8D777B982@EXC-MBX3.cfs.le.ac.uk> Cell/tissue embedded in paraffin wax are as dead as the famous Norwegian Blue parrot, however you could demonstrate programmed cell death, apoptosis. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia Sent: 18 August 2010 14:56 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Test for cell viability in paraffin section? Would you know a techniqe to confirm cell viability in paraffin embedded tissue? Propidium and methylene blue staining are used in cell culturing to detect cell viability. Is there similar technique for detecting cell viability in tissue? This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue? Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Wed Aug 18 09:45:03 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Aug 18 09:45:07 2010 Subject: [Histonet] Microtome alignment Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094819@HPEMX3.HealthPartners.int> We recently purchased the new alignment tool from Source medical products that works on any microtome as it uses a leveling bubble. It is round, so it makes certain all is level from any direction. Works well and has helped us out. I have staff use it daily as it takes minimal time. Only $200. Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From amario3 <@t> uic.edu Wed Aug 18 10:05:28 2010 From: amario3 <@t> uic.edu (Andrea Marion) Date: Wed Aug 18 10:05:36 2010 Subject: [Histonet] Re: muscle stain In-Reply-To: References: <20100818065838.CDE0C1A486C3@barracuda.uic.edu> Message-ID: <80638ec48d9dfce9a1810e4b7378dd14.squirrel@webmail.uic.edu> Hi Saro and others, Our protocol is attached. It is very simple. The only trick is to remember that if you are using this for heart tissue, each section will likely contain cardiomyocytes sectioned at various angles. To accurately measure cell size/diameter, you will want to measure only cells that are neatly cross-sectioned. If you measure cells that are cross-sectioned at an angle, the measurements will be off. This is discussed in the Dolber 1994 paper I mentioned. Essentially you want to identify regions where the WGA staining is very crisp and thin, as this will identify cells that are sectioned perpendicular to their long axis. Cells that are cross-sectioned at some oblique angle will have a fuzzy border of staining along one edge of the cell. This is sometimes hard to identify (at least for me in mouse tissue), and should probably be considered as a caveat to this technique. At the very least, I think it needs to be considered and controlled for. If anyone has a better way of identifying such cells, I'd be happy to hear it. If you are using this technique on skeletal muscle (which I haven't tried), I believe it would be much simpler as the myocytes are aligned more regularly throughout the tissue, and it would be easy to orient the tissue in the block in such a way that you get perfect cross-sectioned myocytes. Wheat Germ Agglutinin-FITC staining to measure myocyte size 1. Begin with 5-8 um FFPE sections on charged slides 2. Dewax slides 3. Rehydrate slides 4. Rinse in PBS 5 minutes x 3 5. Incubate 60 minutes with 100 ug/ml WGA-FITC in PBS + 1 mM CaCl2 6. Rinse in PBS 5 minutes x 3 7. Mount with Vectashield or Vectashield + DAPI 8. Image slides, collecting images of cross-sectioned myocytes 9. Measure area inside WGA-staining for perfectly cross-sectioned myocytes using ImageJ PBS: 8.5 mM Na2HPO4, 1.5 mM KH2PO4, 150 mM NaCl, pH 7.2 WGA-FITC: Vector Labs, Burlingame, CA Product # FL-1021 Andrea Marion Graduate Student University of Illinois - Chicago amario3 uic edu On Wed, August 18, 2010 8:47 am, Bascaramurty, Saro wrote: > Hi Andrea, > > I wouldn't mind if you could email me your protocol. > > Thanks in advance. > > Saro Bascaramurty. > IBD, NRC, Wpg. MB > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea > Marion > Sent: August 18, 2010 8:19 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: muscle stain > > There is an immunofluorescence technique that uses wheat germ agglutinin > (WGA) conjugated to a fluorophore (usually FITC). WGA binds only to the > cell periphery, leaving you with beautiful traces of the outside of each > cell. The cell area is then simple to analyze with ImageJ or any other > software program. It's often used to measure cardiomyocyte size in > hypertrophy studies. I can provide a protocol if requested. > > If you have access to this article, there is a detailed description of the > technique and great images: Regional changes in myocyte structure in model > of canine right atrial hypertrophy. Am J Physiol Heart Circ Physiol 267: > H1279-H1287, 1994. P. C. Dolber, R. P. Bauman, J. C. Rembert and J. C. > Greenfield Jr. > > If not, here is a freely accessible publication. Figure 9, panel B shows > what the result looks like. Cardiomyocyte GATA4 functions as a > stress-responsive regulator of angiogenesis in the murine heart. J Clin > Invest. 2007; 117(11):3198. > http://www.jci.org/articles/view/32573/figure/9 > > Andrea Marion > Graduate Student > University of Illinois - Chicago > amario3 uic edu > >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] muscle stain >> >> I am posting this for a friend. . They would like to stain muscle. >> The eosin and hematoxylin stain gives us too much information since it >> stains the nucleus separately from the cytoplasm and confuses the >> image analysis software. What we need is the measurement of the cell >> diameter only, a simpler stain. Thanks for any info you can give me. >> Deon Simon Any help you can give her on how to measure or a stain will >> be very much appreciated. >> >> Margaret Perry HT(ASCP) >> Dept of Veterinary and Biomedical services Box 2175 South Dakota >> State University Brookings SD 57007 >> 605-688-5638 >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From quakermed <@t> gmail.com Wed Aug 18 11:05:01 2010 From: quakermed <@t> gmail.com (Olivia Lambe) Date: Wed Aug 18 11:05:05 2010 Subject: [Histonet] Advanced Stainers Message-ID: For those of you who have experience with the Bond or Benchmark XT; what do you like/dislike about them? We're looking at advanced stainers as well. Any reoccurring problems you could do without? You don't really hear about those when meeting with company reps. Thank you in advance for any feedback! Olivia From jreichensperger <@t> siumed.edu Wed Aug 18 11:05:47 2010 From: jreichensperger <@t> siumed.edu (Joel Reichensperger) Date: Wed Aug 18 11:05:53 2010 Subject: [Histonet] CD34 positive control Message-ID: <4C6C04DB.80600@siumed.edu> I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) From Erin.Martin <@t> ucsf.edu Wed Aug 18 11:03:39 2010 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Aug 18 11:06:10 2010 Subject: [Histonet] Fontana Message-ID: <379A927A452F3D43A3C8705F4E67905F16546D8C79@EX05.net.ucsf.edu> Hello everone, We recently stained about 20 slides with Fontana for a research project. They were all fine except for 3 or 4 slides on which the pathologist says that the stratum corneum is overstained. The rest of the tissue is fine but that layer is too black. Now I have to fix the problem. Does anyone have an idea of why just that layer would overstain? On only a few slides of the set? Thank you! Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 From sgoebel <@t> xbiotech.com Wed Aug 18 11:15:29 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Aug 18 11:15:36 2010 Subject: [Histonet] CD34 positive control Message-ID: <20100818091529.9e2d9aa830e8449a2412eb1e4f2f067e.720fb2d6ae.wbe@email04.secureserver.net> Blood vessels in tissue. Here is a website that I use for p control list. [1]http://www.pantomics.com/support/IHCcontr There are tons of lists out t Sarah Goebel, B.A., HT (ASCP) H XBiotech USA Inc. 8201 East Ri -------- Original Message -------- Subject: [Histonet] CD34 positive control From: Joel Reichensperger <[2]jreichensperger@siumed.edu> Date: Wed, August 18, 2010 9:05 am To: Histonet <[3]histo I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute [4]jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list [5]Histonet@lists.utsouth [6]http: References 1. 3D"http://www.pantomics.com/ 2. 3D"mailto:jreichensperger@siumed.edu" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:jreichensperger@siumed.edu" 5. 3D"mailto:Histonet@lists.utsouthwestern.edu" 6. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Aug 18 11:17:39 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Aug 18 11:18:17 2010 Subject: [Histonet] CD34 positive control In-Reply-To: <4C6C04DB.80600@siumed.edu> References: <4C6C04DB.80600@siumed.edu> Message-ID: Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensperger@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From elciba <@t> hotmail.com Wed Aug 18 11:28:39 2010 From: elciba <@t> hotmail.com (ricky hachy) Date: Wed Aug 18 11:28:43 2010 Subject: [Histonet] Ventana Renaissance Containers Message-ID: Hello , I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky From JSilverman <@t> NSHS.edu Wed Aug 18 11:30:58 2010 From: JSilverman <@t> NSHS.edu (Silverman, Jeffrey) Date: Wed Aug 18 11:31:10 2010 Subject: [Histonet] CD34 Control Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE41442A3@SYKECHXVS01.nslijhs.net> Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman From luke.perkocha <@t> ucsf.edu Wed Aug 18 12:08:05 2010 From: luke.perkocha <@t> ucsf.edu (Perkocha, Luke) Date: Wed Aug 18 12:11:41 2010 Subject: [Histonet] Frozen commercial controls for IgG, M and A for direct immunofluorescence? Message-ID: Dear Histonet-ers, We currently use tonsil for a positive control tissue for IgG, A and M when we do direct immunofluorescence on skin specimens. We look for occasional plasma cells that stain positive in the cytoplasm, but of course there is no positive ID of what is staining, since the tonsil contains a mixture of plasma cells. Is anyone aware of a commercial source for a control for these reagents that can be used in DIF staining of frozen sections (not paraffin)? Many thanks, Luke Luke.perkocha@ucsf.edu From adam <@t> sensorhealth.com Wed Aug 18 12:14:24 2010 From: adam <@t> sensorhealth.com (Adam Harris) Date: Wed Aug 18 12:14:31 2010 Subject: [Histonet] Ventana Renaissance Containers Message-ID: Hi Ricky, We may have what you need, we have multiple sizes of containers which we can either send empty or can fill for you whichever you prefer. Check out the site if you would like at www.sensorhealth.com Hope that helps. Adam Harris Sales Associate Sensor Health Inc. 110-6 Turnbull Crt. Cambridge, ON N1T 1K6 T: 1-888-777-7080 T: 519-621-1515 F: 519-621-8778 www.sensorhealth.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD34 positive control (McMahon, Loralee A) 2. Ventana Renaissance Containers (ricky hachy) 3. CD34 Control (Silverman, Jeffrey) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger , Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensperger@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 18 Aug 2010 16:28:39 +0000 From: ricky hachy Subject: [Histonet] Ventana Renaissance Containers To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello , I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky ------------------------------ Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: "Silverman, Jeffrey" Subject: [Histonet] CD34 Control To: "'jreichensperger@siumed.edu'" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE41442A3@SYKECHXVS01.nslijhs.net> Content-Type: text/plain; charset="us-ascii" Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 **************************************** From mpence <@t> grhs.net Wed Aug 18 12:19:39 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Aug 18 12:19:47 2010 Subject: [Histonet] Fontana In-Reply-To: <379A927A452F3D43A3C8705F4E67905F16546D8C79@EX05.net.ucsf.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3EAD@is-e2k3.grhs.net> What stain platform are you using? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, August 18, 2010 11:04 AM To: histonet Subject: [Histonet] Fontana Hello everone, We recently stained about 20 slides with Fontana for a research project. They were all fine except for 3 or 4 slides on which the pathologist says that the stratum corneum is overstained. The rest of the tissue is fine but that layer is too black. Now I have to fix the problem. Does anyone have an idea of why just that layer would overstain? On only a few slides of the set? Thank you! Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Aug 18 13:46:55 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Aug 18 13:48:45 2010 Subject: [Histonet] I Have been scammed References: <286916.62275.qm@web180820.mail.gq1.yahoo.com> Message-ID: Glad you're not stuck, Jerry. This is why I act like an old codger and refuse to get a facebook,other social networking sites, etc. account. What's the deal with farmville anyway? :) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jerry Santiago Sent: Tue 8/17/2010 10:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I Have been scammed Histonetters, Thanks for all of your concerns and calls. I was made aware of the Europe scam and have contacted the authorities and they are doing what they suppose to do. I am still in Florida and have not left the country for any reason. My e-mail company states that this was stolen from facebook and gained access to my email account cleaning out all of my contacts and my access to my e-mail account as well as facebook. It will be fine and I apologize for anyone that got caught in this scam. Jerry Santiago Shands Jacksonville Jacksonville, FL 904-244-6149 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Wed Aug 18 16:57:07 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Aug 18 16:57:14 2010 Subject: [Histonet] Advanced Stainers In-Reply-To: References: Message-ID: I really have nothing bad to say about the benchmark. We love ours; it has freed us from having to nurse the IHC bench. You load it and walk away. If there was a down side I would say that the antibodies are a bit on the higher price side. You can find cheaper antibodies but then you have to use their dispensers and they are expensive as well. I guess it depends on what cost you put on tech time?? Is less technical time worth the higher cost?? For us it is a definite yes. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From amosbrooks <@t> gmail.com Wed Aug 18 17:57:03 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Aug 18 17:57:07 2010 Subject: [Histonet] Microtome alignment Message-ID: Hi, My favorite tool works on all microtomes and is priced perfectly. The knobs on the block holder move the block so when it looks like you are going to cut too much of a block you turn them and, voila, a perfectly aligned microtome. Amos Message: 2 Date: Tue, 17 Aug 2010 14:16:26 -0500 From: Sharon.Davis-Devine Subject: [Histonet] Microtome alignment To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From jclark <@t> pcnm.com Wed Aug 18 18:02:30 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Aug 18 18:02:34 2010 Subject: [Histonet] RE: Histonet Digest, Vol 81, Issue 18 Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01318FBE@mail.pcnm.com> Hi Emmanuel, I am a Canadian trained and certified tech working here in the US. You will have to contact the CSMLS (Canadian Society of Medical Laboratory Sciences) to find out were to send your educational info to apply for Canadian certification. You should be able to do a google search and get their website address. You must have Canadian certification to be able to work in any lab and there is probably no point in applying for a job in Canada until you have it. Joanne Clark, HT, MLT (Cdn) Histology Supervisor Pathology Consultants of New Mexico ------------------------------ Message: 2 Date: Sat, 14 Aug 2010 17:37:24 -0500 From: Emmanuel O Subject: [Histonet] Seeking Position in Canada To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I am a US trained and ASCP certified Histologist. I am looking for job opportunity in Canada either as Histologist or as an IHC Specialist. Thanking you all in advance. Emmanuel. ------------------------------ From jclark <@t> pcnm.com Wed Aug 18 18:14:42 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Aug 18 18:14:45 2010 Subject: [Histonet] RE: Histonet Digest, Vol 81, Issue 21 Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01318FBF@mail.pcnm.com> ------------------------------ What we do is color code out microtomes (red, blue, green etc.) and each station has a matching marker. The tech will put a colored stripe down the side of all the blocks that he/she cut. If we have to pull a block out of the file for recuts, we know exactly which machine it was cut on by the color of the stripe on the side of the block. No re-alignment necessary. Joanne Clark, HT Histo Supervisor Path Consultants of NM Message: 2 Date: Tue, 17 Aug 2010 14:16:26 -0500 From: Sharon.Davis-Devine Subject: [Histonet] Microtome alignment To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From atunde90 <@t> yahoo.com Wed Aug 18 18:37:58 2010 From: atunde90 <@t> yahoo.com (Ade Tunde) Date: Wed Aug 18 18:38:04 2010 Subject: [Histonet] RE CD34 CONTROL Message-ID: <693870.16557.qm@web46108.mail.sp1.yahoo.com> hI, Placenta is a very good control for CD34,try it you will like it. Tunde From enrriq88 <@t> yahoo.com Thu Aug 19 08:36:00 2010 From: enrriq88 <@t> yahoo.com (Jaime Plata) Date: Thu Aug 19 08:36:05 2010 Subject: [Histonet] Microtome alignment In-Reply-To: Message-ID: <75704.99678.qm@web113501.mail.gq1.yahoo.com> Amos, indication it is the most practical?answer. Histology it is a combination of skills, art, and science. Jaime E Plata?MD. MT. HTL (ASCP)?? --- On Wed, 8/18/10, Amos Brooks wrote: From: Amos Brooks Subject: [Histonet] Microtome alignment To: Sharon.Davis-Devine@carle.com, histonet@lists.utsouthwestern.edu Date: Wednesday, August 18, 2010, 6:57 PM Hi, ? ???My favorite tool works on all microtomes and is priced perfectly. The knobs on the block holder move the block so when it looks like you are going to cut too much of a block you turn them and, voila, a perfectly aligned microtome. Amos Message: 2 Date: Tue, 17 Aug 2010 14:16:26 -0500 From: Sharon.Davis-Devine Subject: [Histonet] Microtome alignment To: "histonet@lists.utsouthwestern.edu" ? ? ??? Message-ID: ? ? ??? Content-Type: text/plain; charset="us-ascii" We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success.? Does anyone out there have any advice on how to properly align them and what tool to use?? Also, how often do you perform this re-alignment?? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated.? Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology? Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Scouten <@t> leica-microsystems.com Thu Aug 19 09:15:14 2010 From: Charles.Scouten <@t> leica-microsystems.com (Charles.Scouten@leica-microsystems.com) Date: Thu Aug 19 09:15:53 2010 Subject: [Histonet] Re: Blade Angle on Microtome Message-ID: The blade angle should always be so the final bevel face on the bottom of the blade is parallel to the direction of motion. This is discussed in Dr. Peter?s book, ?A Practical guide to frozen sections? Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poole, Kimberly Sent: Thursday, August 12, 2010 3:02 PM To: Subject: [Histonet] Re: Blade Angle on Microtome Hi everyone, I have a really simple question to ask. What angle would you have your blade at on your microtome machine? Right now mine is set to 0 but I am wondering if I should increase this because I am seeing some vibration. Thanks for your help! Kimberly Kimberly Poole B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.poole@drdc-rddc.gc.ca < mailto:kimberly.poole@drdc-rddc.gc.ca> Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From jtaylor <@t> meriter.com Thu Aug 19 09:44:36 2010 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu Aug 19 09:44:41 2010 Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) In-Reply-To: <712c5335-e273-4c8c-9e87-54912c941d7a@EXCA2.meriter.com> Message-ID: Hi Everyone, I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP). Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 18, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD34 positive control (McMahon, Loralee A) 2. Ventana Renaissance Containers (ricky hachy) 3. CD34 Control (Silverman, Jeffrey) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger , Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensperger@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 18 Aug 2010 16:28:39 +0000 From: ricky hachy Subject: [Histonet] Ventana Renaissance Containers To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello , I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky ------------------------------ Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: "Silverman, Jeffrey" Subject: [Histonet] CD34 Control To: "'jreichensperger@siumed.edu'" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE41442A3@SYKECHXVS01.nslijhs.net> Content-Type: text/plain; charset="us-ascii" Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 **************************************** From LSebree <@t> uwhealth.org Thu Aug 19 09:57:30 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Aug 19 09:57:35 2010 Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) In-Reply-To: References: <712c5335-e273-4c8c-9e87-54912c941d7a@EXCA2.meriter.com> Message-ID: <8C023B4AB999614BA4791BAEB26E2738399F68@UWHC-MAIL01.uwhis.hosp.wisc.edu> Hi Jean, We use Cell Marque's (via VMS) clone 24. We have to throw the entire arsenal at our disposal at these stains but they're acceptable. Good luck Jean; any questions, you know where we live! Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Thursday, August 19, 2010 9:45 AM To: 'histonet@lists.utsouthwestern.edu'; 'ihcrg@googlegroups.com' Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) Hi Everyone, I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP). Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 18, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD34 positive control (McMahon, Loralee A) 2. Ventana Renaissance Containers (ricky hachy) 3. CD34 Control (Silverman, Jeffrey) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger , Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensperger@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 18 Aug 2010 16:28:39 +0000 From: ricky hachy Subject: [Histonet] Ventana Renaissance Containers To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello , I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky ------------------------------ Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: "Silverman, Jeffrey" Subject: [Histonet] CD34 Control To: "'jreichensperger@siumed.edu'" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE41442A3@SYKECHXVS01.nslijhs.net> Content-Type: text/plain; charset="us-ascii" Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Aug 19 10:09:04 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Aug 19 10:10:16 2010 Subject: [Histonet] RE: PAX-5 - B-cell specific activator protein (BSAP) In-Reply-To: References: <712c5335-e273-4c8c-9e87-54912c941d7a@EXCA2.meriter.com>, Message-ID: Biocare antibody. Using the diluent that they recommend. High pH retrieval for 20 minutes. And the Dako kit with the mouse linker. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean [jtaylor@meriter.com] Sent: Thursday, August 19, 2010 10:44 AM To: 'histonet@lists.utsouthwestern.edu'; 'ihcrg@googlegroups.com' Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) Hi Everyone, I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP). Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 18, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD34 positive control (McMahon, Loralee A) 2. Ventana Renaissance Containers (ricky hachy) 3. CD34 Control (Silverman, Jeffrey) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger , Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensperger@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 18 Aug 2010 16:28:39 +0000 From: ricky hachy Subject: [Histonet] Ventana Renaissance Containers To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello , I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky ------------------------------ Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: "Silverman, Jeffrey" Subject: [Histonet] CD34 Control To: "'jreichensperger@siumed.edu'" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE41442A3@SYKECHXVS01.nslijhs.net> Content-Type: text/plain; charset="us-ascii" Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kimberly.Poole <@t> drdc-rddc.gc.ca Thu Aug 19 10:28:52 2010 From: Kimberly.Poole <@t> drdc-rddc.gc.ca (Poole, Kimberly) Date: Thu Aug 19 10:28:59 2010 Subject: [Histonet] Re: Staining Protocol Message-ID: <42DFE1A029181B4B8CCBA7261B52D76501455519@suffieldex01.suffield.drdc-rddc.gc.ca> Hello Histonetters, I perform many H&E staining and I think that the previous person who ran the lab, might be missing steps in his staining protocol. Would anyone be willing to share their protocols with me? Thanks for your help! Kimberly Poole B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.poole@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada From GDawson <@t> dynacaremilwaukee.com Thu Aug 19 10:44:06 2010 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Aug 19 10:44:10 2010 Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) In-Reply-To: Message-ID: Another Cell Marque (predilute) customer for PAX-5 here. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Thursday, August 19, 2010 9:45 AM To: 'histonet@lists.utsouthwestern.edu'; 'ihcrg@googlegroups.com' Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) Hi Everyone, I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP). Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 18, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD34 positive control (McMahon, Loralee A) 2. Ventana Renaissance Containers (ricky hachy) 3. CD34 Control (Silverman, Jeffrey) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger , Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensperger@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 18 Aug 2010 16:28:39 +0000 From: ricky hachy Subject: [Histonet] Ventana Renaissance Containers To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello , I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky ------------------------------ Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: "Silverman, Jeffrey" Subject: [Histonet] CD34 Control To: "'jreichensperger@siumed.edu'" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE41442A3@SYKECHXVS01.nslijhs.net> Content-Type: text/plain; charset="us-ascii" Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Thu Aug 19 10:57:55 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Thu Aug 19 10:57:59 2010 Subject: [Histonet] Re: Staining Protocol Message-ID: <20100819085755.9e2d9aa830e8449a2412eb1e4f2f067e.c8007c63da.wbe@email04.secureserver.net> Depends on what kind of hematoxylin you are using and which type eosin (and how dark or "red" the pathologist wants the eosin to be).&nbs plus every HT s bible for years!! Sarah G Histotechnician XBiote 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] Re: Staining Protocol From: "Poole, Kimberly" <[1]Kimberly.Poole@drdc-rddc.gc.ca> Date: Thu, August 19, 2010 8:28 am To: <[2]histonet@lists Hello Histonetters, I perform many H&E staining and I think that the previous person who ra anyone be help! Kimberly Poole [3]B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?velo Medicine Hat, AB, Canada T1A 8K6 [4]kimberly.poole@drdc-rddc. erly.poole@drdc-rddc.gc.ca> Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?co Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list [6]Histonet@lists.utsouth [7]http: References 1. 3D"mailto:Kimberly.Poole@drdc-rddc.gc.c 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"http://B.Sc"/ 4. 3D"mailto:kimberly.poole@drdc-rddc.gc.ca" 5. 3D"mailto:kimberly.poole@drdc-rddc.gc.ca" 6. 3D"mailto:Histonet@lists.utsouthwestern.edu" 7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Sharon.Davis-Devine <@t> carle.com Thu Aug 19 11:10:51 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Thu Aug 19 11:10:56 2010 Subject: [Histonet] Retracting microtomes Message-ID: I have another question for all of you experts out there. We have a brand new microtome that we inherited from another laboratory. It is a retracting microtome whereas all of the other microtomes in our laboratory are not. None of our techs like this new microtome and find it difficult to use. My question to you as a group is, do many of you have retracting microtomes in your laboratory? If so, do you insist that techs in your laboratory learn to use this type of microtome even if they do not feel comfortable using it? The reason I ask is that I want to replace it with an older model which is non-retracting and management is insisting that since we already have it here our techs just need to learn how to use it. We have students rotating thru the rotations and often have to use different microtomes, I am worried that if we have one outlier microtome which operates differently specimens could be compromised. I welcome all your thoughts and opinions. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From akemiat3377 <@t> yahoo.com Thu Aug 19 11:19:23 2010 From: akemiat3377 <@t> yahoo.com (Eileen (Akemi) Allison-Tacha) Date: Thu Aug 19 11:19:26 2010 Subject: [Histonet] Eileen (Akemi) Allison-Tacha wants to stay in touch on LinkedIn Message-ID: <1454131564.3929150.1282234763679.JavaMail.app@ech3-cdn11.prod> LinkedIn ------------ I'd like to add you to my professional network on LinkedIn. - Eileen (Akemi) Allison-Tacha Eileen (Akemi) Allison-Tacha Director at Phoenix Lab Consulting San Francisco Bay Area Confirm that you know Eileen (Akemi) Allison-Tacha https://www.linkedin.com/e/-le17ov-gd1tgzoc-70/isd/1580252622/4TPckY5L/ ------ (c) 2010, LinkedIn Corporation From laurie.colbert <@t> huntingtonhospital.com Thu Aug 19 11:32:33 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Aug 19 11:32:41 2010 Subject: [Histonet] Corrective Action Form Message-ID: <57BE698966D5C54EAE8612E8941D76830957B1EB@EXCHANGE3.huntingtonhospital.com> We recently had a false positive result on one of our immuno stains. I need to write up a report indicating the problem and what the corrective action was. Does anyone have a standard form that they use for Histology problems and resolutions that they would be willing to share with me? Thanks, Laurie Colbert From shultz11 <@t> cox.net Thu Aug 19 11:42:45 2010 From: shultz11 <@t> cox.net (shultz11@cox.net) Date: Thu Aug 19 11:42:46 2010 Subject: [Histonet] Job Message-ID: <20100819124245.R3ST5.1265637.imail@eastrmwml38> Full time Histology Position at Louisiana State University in Baton Rouge,LA . Apply online at www.lsu.edu. From jtaylor <@t> meriter.com Thu Aug 19 11:43:48 2010 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu Aug 19 11:43:52 2010 Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) In-Reply-To: 8C023B4AB999614BA4791BAEB26E2738399F68@UWHC-MAIL01.uwhis.hosp.wisc.edu References: 712c5335-e273-4c8c-9e87-54912c941d7a@EXCA2.meriter.com B9AF5D89FC58AF4CA27505DB709849630AB4138D44@EXVS1.meriter.com 8C023B4AB999614BA4791BAEB26E2738399F68@UWHC-MAIL01.uwhis.hosp.wisc.edu Message-ID: <20100819164348.22790.qmail@dusk.parklogic.com> Dear Sir/Madam, Your email was unable reach the intended person that you were sending it to. For more information on our business please click on the following link: [1]Click here for our website We look forward to your continued business in the future. Regards, Webmaster References 1. http://www.downwind.com.au/avdir/rd.php?id=7564 From Heather.Rojas <@t> va.gov Thu Aug 19 11:45:24 2010 From: Heather.Rojas <@t> va.gov (Rojas, Heather L.) Date: Thu Aug 19 11:45:32 2010 Subject: [Histonet] histonet post Message-ID: <58AB3FEE2C2E3846B2F955400BB25DA006113D38@VHAV22MSGA3.v22.med.va.gov> Could you please post the following announcement: VA Medical Center, Loma Linda, CA is seeking applicants for a Histology Supervisor. The department consists of three histotechnologists and two cytology/histology technicians and has fully automated immunohistochemistry, special stains, H&E stains and cover slipping. The responsibilities of this position include supervising the daily operations of the histology department including staff training, competency assessment, performance reviews, maintenance of and adherence to standard operating procedures, routine quality assurance, adherence to CAP and JCAHO standards, and selection and implementation of new instrumentation and equipment. This position is a working supervisory position, and it is expected that the supervisor will regularly perform bench work including embedding, microtomy, special stains and immunohistochemistry, with certain days set aside solely for supervisory duties. Applicants should have supervisory experience, solid interpersonal skills, strong immunohistochemical technical skills, knowledge of CAP and JCAHO regulations, good written communication ability, and a solid knowledge of histology. ASCP Histology Technician certification and US Citizenship are required. Position is subject to random drug testing. This agency provides reasonable accommodations to applicants with disabilities where appropriate. For further information from Dr. Heather Rojas at (909) 825-7084 x2586 or by email: heather.rojas@va.gov Qualified candidates may send resume to: Anna DeRito, HR Specialist (136-05S) Jerry L. Pettis VAMC 11201 Benton Street Loma Linda, CA 92357 From jtaylor <@t> meriter.com Thu Aug 19 12:25:41 2010 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu Aug 19 12:27:52 2010 Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) In-Reply-To: 8C023B4AB999614BA4791BAEB26E2738399F68@UWHC-MAIL01.uwhis.hosp.wisc.edu References: 712c5335-e273-4c8c-9e87-54912c941d7a@EXCA2.meriter.com B9AF5D89FC58AF4CA27505DB709849630AB4138D44@EXVS1.meriter.com 8C023B4AB999614BA4791BAEB26E2738399F68@UWHC-MAIL01.uwhis.hosp.wisc.edu Message-ID: <20100819172541.3434.qmail@dusk.parklogic.com> Dear Sir/Madam, Your email was unable reach the intended person that you were sending it to. For more information on our business please click on the following link: [1]Click here for our website We look forward to your continued business in the future. Regards, Webmaster References 1. http://www.downwind.com.au/avdir/rd.php?id=7564 From jtaylor <@t> meriter.com Thu Aug 19 13:01:22 2010 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu Aug 19 13:01:25 2010 Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) In-Reply-To: 8C023B4AB999614BA4791BAEB26E2738399F68@UWHC-MAIL01.uwhis.hosp.wisc.edu References: 712c5335-e273-4c8c-9e87-54912c941d7a@EXCA2.meriter.com B9AF5D89FC58AF4CA27505DB709849630AB4138D44@EXVS1.meriter.com 8C023B4AB999614BA4791BAEB26E2738399F68@UWHC-MAIL01.uwhis.hosp.wisc.edu Message-ID: <20100819180122.15940.qmail@dusk.parklogic.com> Dear Sir/Madam, Your email was unable reach the intended person that you were sending it to. For more information on our business please click on the following link: [1]Click here for our website We look forward to your continued business in the future. Regards, Webmaster References 1. http://www.downwind.com.au/avdir/rd.php?id=7564 From Herrick.James <@t> mayo.edu Thu Aug 19 14:22:33 2010 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Thu Aug 19 14:22:37 2010 Subject: [Histonet] Staining Question Message-ID: <7267A64D75F58241B577876D8A885631038FE276@msgebe41> Good afternoon everybody, Would anyone be willing to offer their wisdom on the best etching solution for 50 to 75 micron thick jaw specimens embedded in MMA? The stain we want to use is a Trichrome. Thanks in advance for your help. Jim From ratliffjack <@t> hotmail.com Thu Aug 19 14:28:10 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Aug 19 14:28:29 2010 Subject: [Histonet] Staining Question In-Reply-To: <7267A64D75F58241B577876D8A885631038FE276@msgebe41> References: <7267A64D75F58241B577876D8A885631038FE276@msgebe41> Message-ID: 0.7% Formic Acid for 30 seconds with sonication, then rinse well in DI water. Jack On Aug 19, 2010, at 2:22 PM, "Herrick, James L. (Jim)" wrote: > Good afternoon everybody, > > Would anyone be willing to offer their wisdom on the best etching > solution for 50 to 75 micron thick jaw specimens embedded in MMA? The > stain we want to use is a Trichrome. Thanks in advance for your help. > > Jim > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jtaylor <@t> meriter.com Thu Aug 19 14:39:52 2010 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu Aug 19 14:39:56 2010 Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) In-Reply-To: 8C023B4AB999614BA4791BAEB26E2738399F68@UWHC-MAIL01.uwhis.hosp.wisc.edu References: 712c5335-e273-4c8c-9e87-54912c941d7a@EXCA2.meriter.com B9AF5D89FC58AF4CA27505DB709849630AB4138D44@EXVS1.meriter.com 8C023B4AB999614BA4791BAEB26E2738399F68@UWHC-MAIL01.uwhis.hosp.wisc.edu Message-ID: <20100819193952.23059.qmail@dusk.parklogic.com> Dear Sir/Madam, Your email was unable reach the intended person that you were sending it to. For more information on our business please click on the following link: [1]Click here for our website We look forward to your continued business in the future. Regards, Webmaster References 1. http://www.downwind.com.au/avdir/rd.php?id=7564 From rjbuesa <@t> yahoo.com Thu Aug 19 14:47:28 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 19 14:47:33 2010 Subject: [Histonet] Corrective Action Form In-Reply-To: <57BE698966D5C54EAE8612E8941D76830957B1EB@EXCHANGE3.huntingtonhospital.com> Message-ID: <846706.10692.qm@web65702.mail.ac4.yahoo.com> Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and?they have to be approved by the HR?Dept. Ren? J.? --- On Thu, 8/19/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Corrective Action Form To: histonet@lists.utsouthwestern.edu Date: Thursday, August 19, 2010, 12:32 PM We recently had a false positive result on one of our immuno stains.? I need to write up a report indicating the problem and what the corrective action was.? Does anyone have a standard form that they use for Histology problems and resolutions that they would be willing to share with me? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Thu Aug 19 15:01:05 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Aug 19 15:01:11 2010 Subject: [Histonet] Gram Control Message-ID: Would anyone want to trade for a gram control? I have lots of H. pylori control blocks made up. I could trade for other things too... Thanks! Mark From lblazek <@t> digestivespecialists.com Thu Aug 19 15:02:46 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Aug 19 15:03:03 2010 Subject: [Histonet] Corrective Action Form In-Reply-To: <846706.10692.qm@web65702.mail.ac4.yahoo.com> References: <57BE698966D5C54EAE8612E8941D76830957B1EB@EXCHANGE3.huntingtonhospital.com> <846706.10692.qm@web65702.mail.ac4.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EAF72C73C@IBMB7Exchange.digestivespecialists.com> Corrective actions don't have to lead to punishment! Corrective action should be a positive quality assurance. How can we make it better and not have this happen this way again type of thing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, August 19, 2010 3:47 PM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: Re: [Histonet] Corrective Action Form Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and?they have to be approved by the HR?Dept. Ren? J.? --- On Thu, 8/19/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Corrective Action Form To: histonet@lists.utsouthwestern.edu Date: Thursday, August 19, 2010, 12:32 PM We recently had a false positive result on one of our immuno stains.? I need to write up a report indicating the problem and what the corrective action was.? Does anyone have a standard form that they use for Histology problems and resolutions that they would be willing to share with me? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Aug 19 16:26:26 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Aug 19 16:26:31 2010 Subject: [Histonet] Corrective Action Form Message-ID: <57BE698966D5C54EAE8612E8941D76830957B29D@EXCHANGE3.huntingtonhospital.com> I'm looking at it from the standpoint of an inspector - we had a problem and what steps did we follow to correct it and prevent it from happening again. I'm not planning on using it as a punishment for anyone. ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, August 19, 2010 12:47 PM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: Re: [Histonet] Corrective Action Form Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and they have to be approved by the HR Dept. Ren? J. --- On Thu, 8/19/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Corrective Action Form To: histonet@lists.utsouthwestern.edu Date: Thursday, August 19, 2010, 12:32 PM We recently had a false positive result on one of our immuno stains. I need to write up a report indicating the problem and what the corrective action was. Does anyone have a standard form that they use for Histology problems and resolutions that they would be willing to share with me? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Aug 19 16:55:40 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Aug 19 16:55:46 2010 Subject: [Histonet] Corrective Action Form In-Reply-To: <57BE698966D5C54EAE8612E8941D76830957B29D@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D76830957B29D@EXCHANGE3.huntingtonhospital.com> Message-ID: <727AD5E9-B8BB-459E-BB1A-E615B213D8A1@yahoo.com> This is used in concordance to CAP guidelines for PIP. The phrasing scares people off because ii states "Incident/Complaint Form". People need to realize we ALL make mistakes. This form is used for corrective measures and process improvement in Pre-Analytical- Technical-Analytical & Post-Analytical. It is recommended to have Quarterly Reviews by Committee members to address what Corrective Measures have been implemented. Remember folks, this is for improving patient care and not to finger point or put blame on anyone. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Aug 19, 2010, at 2:26 PM, Laurie Colbert wrote: > I'm looking at it from the standpoint of an inspector - we had a > problem and what steps did we follow to correct it and prevent it > from happening again. I'm not planning on using it as a punishment > for anyone. > > > > ________________________________ > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, August 19, 2010 12:47 PM > To: histonet@lists.utsouthwestern.edu; Laurie Colbert > Subject: Re: [Histonet] Corrective Action Form > > > > Probably many of us have but it would be better if you consult with > your HR Dept. because corrective actions could lead to some type of > "punishment" and they have to be approved by the HR Dept. > > Ren? J. > > --- On Thu, 8/19/10, Laurie Colbert > wrote: > > > From: Laurie Colbert > Subject: [Histonet] Corrective Action Form > To: histonet@lists.utsouthwestern.edu > Date: Thursday, August 19, 2010, 12:32 PM > > We recently had a false positive result on one of our immuno > stains. I > need to write up a report indicating the problem and what the > corrective > action was. Does anyone have a standard form that they use for > Histology problems and resolutions that they would be willing to > share > with me? > > > > Thanks, > > Laurie Colbert > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu mc/compose?to=Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Aug 19 16:59:05 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Aug 19 16:59:26 2010 Subject: [Histonet] Adenovirus controls Message-ID: <74BDD9F3D52541C08EDF888BAAE3F85B@JoePC> Greetings histoland, does any one know of a company that sells adenovirus controls slides? I've tried NewComer's Supply, MasterTech, Biocare, Biogenex, ThermoFisherRichard Allen, or whatever they're called this week. Thanks Joe From mmccoy <@t> chmca.org Thu Aug 19 17:05:25 2010 From: mmccoy <@t> chmca.org (McCoy, Marilynn) Date: Thu Aug 19 17:05:21 2010 Subject: [Histonet] Job posting for Akron Children's Hospital Message-ID: Would you like an opportunity to use your versatile skills in a great lab environment? Akron Children's Hospital, located in Northeast Ohio, has a part-time opening for a Histology Technician - 20 hours per week, variable, day shift. Qualifications: Preferred - Associate degree in Histology with certification or eligibility for certification by ASCP or minimum of three years previous experience as a Histotech. Also Considered - Associate degree in Medical Laboratory Technology with certification or a BS or Masters in a scientific area such as Medical Technology and ability to learn and perform histology/Diener skills. Histology/Electron Microscopy and/or Diener experience preferred. Check out the opportunity at https://www.healthcaresource.com/akronchildren/index.cfm?fuseaction=search.jobDetails&template=dsp_job_details.cfm&cJobId=955099 Or contact Marilynn K. McCoy Employment Specialist Akron Children's Hospital One Perkins Square Akron, OH 44308-1062 330-543-3443 phone 330-543-3176 fax From Maria.Katleba <@t> stjoe.org Thu Aug 19 17:19:03 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Aug 19 17:30:19 2010 Subject: [Histonet] Corrective Action Form In-Reply-To: <846706.10692.qm@web65702.mail.ac4.yahoo.com> References: <57BE698966D5C54EAE8612E8941D76830957B1EB@EXCHANGE3.huntingtonhospital.com> <846706.10692.qm@web65702.mail.ac4.yahoo.com> Message-ID: You don't want to call it corrective action, as that indicates 'negligence' or 'failure to follow direction' on the part of the tech... Unless that is the case and you are looking to go after an employee...... Instead you need to call it Performance Improvement... This notes a problem, reason for the problem, and a solution to the problem... followed up by a time line where you follow the change in Policy and Procedure. It shows a problem that occurred where patient safety was compromised and a solution to the problem to avoid any potential future. You document this... Say you change the procedure today... well, keep track of the IHCs for the next 2 months...6 months (what ever timeline you choose)... then write up the conclusion to your findings. Here's and example: "The change to preparing IHC slides per Policy and Procedure #2001 on May 1, 2010 and studied for 4 months, we found no more issues regarding false positives." Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, August 19, 2010 12:47 PM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: Re: [Histonet] Corrective Action Form Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and they have to be approved by the HR Dept. Ren? J. --- On Thu, 8/19/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Corrective Action Form To: histonet@lists.utsouthwestern.edu Date: Thursday, August 19, 2010, 12:32 PM We recently had a false positive result on one of our immuno stains. I need to write up a report indicating the problem and what the corrective action was. Does anyone have a standard form that they use for Histology problems and resolutions that they would be willing to share with me? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From kimtournear <@t> yahoo.com Thu Aug 19 19:04:57 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Aug 19 19:05:02 2010 Subject: Fw: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) Message-ID: <811192.15635.qm@web54207.mail.re2.yahoo.com> ~Kim~ ~Don't be afraid your life will?end, be afraid it will never begin~ OU Rocks!!!! --- On Thu, 8/19/10, Kim Tournear wrote: From: Kim Tournear Subject: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) To: "Sebree Linda A" Date: Thursday, August 19, 2010, 5:02 PM Bond Max or Bond III users: ? BD Biosciences (clone 24) 1:35 with?ER1 for 20 minutes (low pH) Biocare (BC/24) 1:50 with ER2 for 20 minutes (high pH) VBS (clone 1EW) 1:150 with ER2 for 25 minutes (high pH) VBS (clone 1EW) 1:40 with ER1 for 20 minutes (low pH) Biocare (clone BC/24) 1:50 with ER2 for 20 minutes (high pH) ? These protocols come from various sites using the Bond instrument.... Tonsil is the control of choice. ? Hope this helps..... ~Kim~ ~Don't be afraid your life will?end, be afraid it will never begin~ OU Rocks!!!! --- On Thu, 8/19/10, Sebree Linda A wrote: From: Sebree Linda A Subject: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) To: "Taylor, Jean" , histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com Date: Thursday, August 19, 2010, 7:57 AM Hi Jean, We use Cell Marque's (via VMS) clone 24.? We have to throw the entire arsenal at our disposal at these stains but they're acceptable. Good luck Jean; any questions, you know where we live! Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Thursday, August 19, 2010 9:45 AM To: 'histonet@lists.utsouthwestern.edu'; 'ihcrg@googlegroups.com' Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) Hi Everyone, I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP). Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 18, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to ? ? ? ? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ? ? ? ? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ? ? ? ? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ? ? ? ? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. RE: CD34 positive control (McMahon, Loralee A) ???2. Ventana Renaissance Containers (ricky hachy) ???3. CD34 Control (Silverman, Jeffrey) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger , Histonet ? ? ? ? Message-ID: Content-Type: text/plain; charset="us-ascii" Just about any tissue should work.???It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensperger@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control ? I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 18 Aug 2010 16:28:39 +0000 From: ricky hachy Subject: [Histonet] Ventana Renaissance Containers To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello , I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky ------------------------------ Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: "Silverman, Jeffrey" Subject: [Histonet] CD34 Control To: "'jreichensperger@siumed.edu'" Cc: "'histonet@lists.utsouthwestern.edu'" ? ? ? ? Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE41442A3@SYKECHXVS01.nslijhs.net> Content-Type: text/plain; charset="us-ascii" Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis,? are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rhbrown1 <@t> histocs.com Thu Aug 19 21:12:38 2010 From: rhbrown1 <@t> histocs.com (Leroy Brown) Date: Thu Aug 19 21:18:24 2010 Subject: [Histonet] looking for any type of used paraffin block files Message-ID: I am looking for paraffin block files. If you have some you no longer use or want I would be happy to talk to you. The files are for my personal use in my lab. Not for selling etc. Please, no equipment dealers or suppliers. I am looking for used. thanks for your help. LeRoy Brown HT(ASCP) HTL HCS, Inc. Everson, WA 98247 360-966-7300 From mwfolsom <@t> rgbio.com Thu Aug 19 23:02:37 2010 From: mwfolsom <@t> rgbio.com (Michael Folsom) Date: Thu Aug 19 22:57:52 2010 Subject: [Histonet] Retracting microtomes In-Reply-To: References: Message-ID: <1282276957.3894.7.camel@chico.322tulane.org> Hi: Have you considered the possibility that there is something wrong with the microtome? Sadly equipment that is "inherited" can have issues. Perhaps a good cleaning is in order - By the way, what the make/model of the tome? I have a couple of B&L's that are available for a trade --;-) Later - Mike Rio Grande Biological Albuquerque, NM 87106 On Thu, 2010-08-19 at 11:10 -0500, Sharon.Davis-Devine wrote: > I have another question for all of you experts out there. We have a brand new microtome that we inherited from another laboratory. It is a retracting microtome whereas all of the other microtomes in our laboratory are not. None of our techs like this new microtome and find it difficult to use. My question to you as a group is, do many of you have retracting microtomes in your laboratory? If so, do you insist that techs in your laboratory learn to use this type of microtome even if they do not feel comfortable using it? > > The reason I ask is that I want to replace it with an older model which is non-retracting and management is insisting that since we already have it here our techs just need to learn how to use it. We have students rotating thru the rotations and often have to use different microtomes, I am worried that if we have one outlier microtome which operates differently specimens could be compromised. I welcome all your thoughts and opinions. > > Sharon Davis-Devine, CT (ASCP) > Cytology-Histology Supervisor > Carle Foundation Hospital > Laboratory and Pathology Services > 611 West Park Street > Urbana, Illinois 61801 > 217-383-3572 > sharon.davis-devine@carle.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Aug 20 04:12:56 2010 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Aug 20 04:13:01 2010 Subject: [Histonet] Corrective Action Form In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EAF72C73C@IBMB7Exchange.digestivespecialists.com> References: <57BE698966D5C54EAE8612E8941D76830957B1EB@EXCHANGE3.huntingtonhospital.com>, <846706.10692.qm@web65702.mail.ac4.yahoo.com>, <5A2BD13465E061429D6455C8D6B40E390EAF72C73C@IBMB7Exchange.digestivespecialists.com> Message-ID: How true, but a lot of people don't understand that and get it all wrong! The inspectors just want to see the identification of problems, the follow-up and the resolution. The feedback loop. -Joelle > From: lblazek@digestivespecialists.com > To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; laurie.colbert@huntingtonhospital.com > Date: Thu, 19 Aug 2010 16:02:46 -0400 > Subject: RE: [Histonet] Corrective Action Form > CC: > > Corrective actions don't have to lead to punishment! Corrective action should be a positive quality assurance. How can we make it better and not have this happen this way again type of thing. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, August 19, 2010 3:47 PM > To: histonet@lists.utsouthwestern.edu; Laurie Colbert > Subject: Re: [Histonet] Corrective Action Form > > Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and they have to be approved by the HR Dept. > Ren? J. > > --- On Thu, 8/19/10, Laurie Colbert wrote: > > > From: Laurie Colbert > Subject: [Histonet] Corrective Action Form > To: histonet@lists.utsouthwestern.edu > Date: Thursday, August 19, 2010, 12:32 PM > > > We recently had a false positive result on one of our immuno stains. I > need to write up a report indicating the problem and what the corrective > action was. Does anyone have a standard form that they use for > Histology problems and resolutions that they would be willing to share > with me? > > > > Thanks, > > Laurie Colbert > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Aug 20 07:07:30 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Aug 20 07:07:46 2010 Subject: [Histonet] job opening Message-ID: <5A2BD13465E061429D6455C8D6B40E390EAF72C73F@IBMB7Exchange.digestivespecialists.com> We have a job opening for a full time days certified histotech in the Dayton, Ohio area. We have a great team and state of the art equipment. Please feel free to contact me for more information. Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com From Ronald.Houston <@t> nationwidechildrens.org Fri Aug 20 07:33:31 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Aug 20 07:33:45 2010 Subject: [Histonet] cpt code for fused twin placentas Message-ID: Can you charge 88307 x2 for fused twin placentas? I'm not sure as strictly only one specimen was received, but our billing company says yes? Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From JWeems <@t> sjha.org Fri Aug 20 09:12:08 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Aug 20 09:12:15 2010 Subject: [Histonet] RE: cpt code for fused twin placentas In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164033215FD25@CHEXCMS10.one.ads.che.org> Yes, if the pathologist is giving two separate diagnoses. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, August 20, 2010 08:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cpt code for fused twin placentas Can you charge 88307 x2 for fused twin placentas? I'm not sure as strictly only one specimen was received, but our billing company says yes? Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sgoebel <@t> xbiotech.com Fri Aug 20 09:36:58 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Aug 20 09:37:05 2010 Subject: [Histonet] Hawaii Message-ID: <20100820073658.9e2d9aa830e8449a2412eb1e4f2f067e.58c9ef4452.wbe@email04.secureserver.net> Does anyone know of any open positions in Hawaii, and what is the expected payscale there? I have a BA, HT, and a very good knowledge of IHC. I have 6 years registered experience with 2 years histo. lab nd my fiance might be moving there because of his job. Any help would Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 [DEL: (5 :DEL] From jclark <@t> pcnm.com Fri Aug 20 12:20:37 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Fri Aug 20 12:20:43 2010 Subject: [Histonet] RE: cpt code for fused twin placentas Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01319217@mail.pcnm.com> We charge for two if when the specimens are received there is a clip on one of the placental cords. The same for twin placenta's that aren't fused, if there is a way to distinguish between the two, we bill two. Message: 19 Date: Fri, 20 Aug 2010 08:33:31 -0400 From: "Houston, Ronald" Subject: [Histonet] cpt code for fused twin placentas To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Can you charge 88307 x2 for fused twin placentas? I'm not sure as strictly only one specimen was received, but our billing company says yes? Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From rsrichmond <@t> gmail.com Fri Aug 20 12:20:41 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Aug 20 12:20:48 2010 Subject: [Histonet] Re: CPT code for fused twin placentas Message-ID: The CPT code for a term or near-term placenta is 88307. With twin placentas, you may bill 88307 x2 if it is possible to distinguish which cord belongs to twin A and which to twin B. Makes no difference whether the placentas are fused or not. The obstetrician may mark A and B with clips or ties or whatnot, any way you can make the distinction. The pathologist may use one diagnosis line, as long as the gross description is clear. My sign-out might be: placenta (cesarean section): fused dichorionic diamniotic twin placentas. No chorioamnionitis or meconium lesion seen. Bob Richmond Samurai Pathologist Knoxville TN From john <@t> imebinc.com Fri Aug 20 13:08:41 2010 From: john <@t> imebinc.com (John O'Brien) Date: Fri Aug 20 13:02:23 2010 Subject: [Histonet] RE: Histonet Digest, Vol 81, Issue 26 Message-ID: <004f01cb4092$b8ea5940$4a01a8c0@EXECUTIVE01> Dawn, Note 15 and 16 may be some business, I believe we have some used block storage system in back ,check with David, Sakura models Joh -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) (Taylor, Jean) 2. [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) (Taylor, Jean) 3. Staining Question (Herrick, James L. (Jim)) 4. Re: Staining Question (Jack Ratliff) 5. [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) (Taylor, Jean) 6. Re: Corrective Action Form (Rene J Buesa) 7. Gram Control (Mark Tarango) 8. RE: Corrective Action Form (Blazek, Linda) 9. RE: Corrective Action Form (Laurie Colbert) 10. Re: Corrective Action Form (Akemi Allison) 11. Adenovirus controls (Joe Nocito) 12. Job posting for Akron Children's Hospital (McCoy, Marilynn) 13. RE: Corrective Action Form (Maria Katleba) 14. Fw: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) (Kim Tournear) 15. looking for any type of used paraffin block files (Leroy Brown) 16. Re: Retracting microtomes (Michael Folsom) 17. RE: Corrective Action Form (joelle weaver) 18. job opening (Blazek, Linda) 19. cpt code for fused twin placentas (Houston, Ronald) 20. RE: cpt code for fused twin placentas (Weems, Joyce) 21. Hawaii (sgoebel@xbiotech.com) ---------------------------------------------------------------------- Message: 1 Date: Fri, 20 Aug 2010 03:25:41 +1000 From: "Taylor, Jean" Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) To: "Sebree Linda A" Cc: histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com Message-ID: <20100819172541.3434.qmail@dusk.parklogic.com> Content-Type: text/plain; boundary="1282238741.aC6ACbF0.3413"; charset="us-ascii" Dear Sir/Madam, Your email was unable reach the intended person that you were sending it to. For more information on our business please click on the following link: [1]Click here for our website We look forward to your continued business in the future. Regards, Webmaster References 1. http://www.downwind.com.au/avdir/rd.php?id=7564 ------------------------------ Message: 2 Date: Fri, 20 Aug 2010 04:01:22 +1000 From: "Taylor, Jean" Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) To: "Sebree Linda A" Cc: histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com Message-ID: <20100819180122.15940.qmail@dusk.parklogic.com> Content-Type: text/plain; boundary="1282240882.203a0.15929"; charset="us-ascii" Dear Sir/Madam, Your email was unable reach the intended person that you were sending it to. For more information on our business please click on the following link: [1]Click here for our website We look forward to your continued business in the future. Regards, Webmaster References 1. http://www.downwind.com.au/avdir/rd.php?id=7564 ------------------------------ Message: 3 Date: Thu, 19 Aug 2010 14:22:33 -0500 From: "Herrick, James L. (Jim)" Subject: [Histonet] Staining Question To: Message-ID: <7267A64D75F58241B577876D8A885631038FE276@msgebe41> Content-Type: text/plain; charset="us-ascii" Good afternoon everybody, Would anyone be willing to offer their wisdom on the best etching solution for 50 to 75 micron thick jaw specimens embedded in MMA? The stain we want to use is a Trichrome. Thanks in advance for your help. Jim ------------------------------ Message: 4 Date: Thu, 19 Aug 2010 14:28:10 -0500 From: Jack Ratliff Subject: Re: [Histonet] Staining Question To: "Herrick, James L. (Jim)" Cc: "" Message-ID: Content-Type: text/plain; charset=us-ascii 0.7% Formic Acid for 30 seconds with sonication, then rinse well in DI water. Jack On Aug 19, 2010, at 2:22 PM, "Herrick, James L. (Jim)" wrote: > Good afternoon everybody, > > Would anyone be willing to offer their wisdom on the best etching > solution for 50 to 75 micron thick jaw specimens embedded in MMA? The > stain we want to use is a Trichrome. Thanks in advance for your help. > > Jim > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Fri, 20 Aug 2010 05:39:52 +1000 From: "Taylor, Jean" Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) To: "Sebree Linda A" Cc: histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com Message-ID: <20100819193952.23059.qmail@dusk.parklogic.com> Content-Type: text/plain; boundary="1282246792.5b22a0.23037"; charset="us-ascii" Dear Sir/Madam, Your email was unable reach the intended person that you were sending it to. For more information on our business please click on the following link: [1]Click here for our website We look forward to your continued business in the future. Regards, Webmaster References 1. http://www.downwind.com.au/avdir/rd.php?id=7564 ------------------------------ Message: 6 Date: Thu, 19 Aug 2010 12:47:28 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Corrective Action Form To: histonet@lists.utsouthwestern.edu, Laurie Colbert Message-ID: <846706.10692.qm@web65702.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and?they have to be approved by the HR?Dept. Ren? J.? --- On Thu, 8/19/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Corrective Action Form To: histonet@lists.utsouthwestern.edu Date: Thursday, August 19, 2010, 12:32 PM We recently had a false positive result on one of our immuno stains.? I need to write up a report indicating the problem and what the corrective action was.? Does anyone have a standard form that they use for Histology problems and resolutions that they would be willing to share with me? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 19 Aug 2010 13:01:05 -0700 From: Mark Tarango Subject: [Histonet] Gram Control To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Would anyone want to trade for a gram control? I have lots of H. pylori control blocks made up. I could trade for other things too... Thanks! Mark ------------------------------ Message: 8 Date: Thu, 19 Aug 2010 16:02:46 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] Corrective Action Form To: 'Rene J Buesa' , "histonet@lists.utsouthwestern.edu" , Laurie Colbert Message-ID: <5A2BD13465E061429D6455C8D6B40E390EAF72C73C@IBMB7Exchange.digestivespeci alists.com> Content-Type: text/plain; charset="iso-8859-1" Corrective actions don't have to lead to punishment! Corrective action should be a positive quality assurance. How can we make it better and not have this happen this way again type of thing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, August 19, 2010 3:47 PM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: Re: [Histonet] Corrective Action Form Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and?they have to be approved by the HR?Dept. Ren? J.? --- On Thu, 8/19/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Corrective Action Form To: histonet@lists.utsouthwestern.edu Date: Thursday, August 19, 2010, 12:32 PM We recently had a false positive result on one of our immuno stains.? I need to write up a report indicating the problem and what the corrective action was.? Does anyone have a standard form that they use for Histology problems and resolutions that they would be willing to share with me? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 19 Aug 2010 14:26:26 -0700 From: "Laurie Colbert" Subject: RE: [Histonet] Corrective Action Form To: "Rene J Buesa" , Message-ID: <57BE698966D5C54EAE8612E8941D76830957B29D@EXCHANGE3.huntingtonhospital.c om> Content-Type: text/plain; charset="iso-8859-1" I'm looking at it from the standpoint of an inspector - we had a problem and what steps did we follow to correct it and prevent it from happening again. I'm not planning on using it as a punishment for anyone. ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, August 19, 2010 12:47 PM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: Re: [Histonet] Corrective Action Form Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and they have to be approved by the HR Dept. Ren? J. --- On Thu, 8/19/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Corrective Action Form To: histonet@lists.utsouthwestern.edu Date: Thursday, August 19, 2010, 12:32 PM We recently had a false positive result on one of our immuno stains. I need to write up a report indicating the problem and what the corrective action was. Does anyone have a standard form that they use for Histology problems and resolutions that they would be willing to share with me? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 19 Aug 2010 14:55:40 -0700 From: Akemi Allison Subject: Re: [Histonet] Corrective Action Form To: "Laurie Colbert" Cc: histonet@lists.utsouthwestern.edu Message-ID: <727AD5E9-B8BB-459E-BB1A-E615B213D8A1@yahoo.com> Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed This is used in concordance to CAP guidelines for PIP. The phrasing scares people off because ii states "Incident/Complaint Form". People need to realize we ALL make mistakes. This form is used for corrective measures and process improvement in Pre-Analytical- Technical-Analytical & Post-Analytical. It is recommended to have Quarterly Reviews by Committee members to address what Corrective Measures have been implemented. Remember folks, this is for improving patient care and not to finger point or put blame on anyone. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Aug 19, 2010, at 2:26 PM, Laurie Colbert wrote: > I'm looking at it from the standpoint of an inspector - we had a > problem and what steps did we follow to correct it and prevent it > from happening again. I'm not planning on using it as a punishment > for anyone. > > > > ________________________________ > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, August 19, 2010 12:47 PM > To: histonet@lists.utsouthwestern.edu; Laurie Colbert > Subject: Re: [Histonet] Corrective Action Form > > > > Probably many of us have but it would be better if you consult with > your HR Dept. because corrective actions could lead to some type of > "punishment" and they have to be approved by the HR Dept. > > Ren? J. > > --- On Thu, 8/19/10, Laurie Colbert > wrote: > > > From: Laurie Colbert > Subject: [Histonet] Corrective Action Form > To: histonet@lists.utsouthwestern.edu > Date: Thursday, August 19, 2010, 12:32 PM > > We recently had a false positive result on one of our immuno > stains. I > need to write up a report indicating the problem and what the > corrective > action was. Does anyone have a standard form that they use for > Histology problems and resolutions that they would be willing to > share > with me? > > > > Thanks, > > Laurie Colbert > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu mc/compose?to=Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 19 Aug 2010 16:59:05 -0500 From: "Joe Nocito" Subject: [Histonet] Adenovirus controls To: "Histonet" Message-ID: <74BDD9F3D52541C08EDF888BAAE3F85B@JoePC> Content-Type: text/plain; charset="iso-8859-1" Greetings histoland, does any one know of a company that sells adenovirus controls slides? I've tried NewComer's Supply, MasterTech, Biocare, Biogenex, ThermoFisherRichard Allen, or whatever they're called this week. Thanks Joe ------------------------------ Message: 12 Date: Thu, 19 Aug 2010 18:05:25 -0400 From: "McCoy, Marilynn" Subject: [Histonet] Job posting for Akron Children's Hospital To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Would you like an opportunity to use your versatile skills in a great lab environment? Akron Children's Hospital, located in Northeast Ohio, has a part-time opening for a Histology Technician - 20 hours per week, variable, day shift. Qualifications: Preferred - Associate degree in Histology with certification or eligibility for certification by ASCP or minimum of three years previous experience as a Histotech. Also Considered - Associate degree in Medical Laboratory Technology with certification or a BS or Masters in a scientific area such as Medical Technology and ability to learn and perform histology/Diener skills. Histology/Electron Microscopy and/or Diener experience preferred. Check out the opportunity at https://www.healthcaresource.com/akronchildren/index.cfm?fuseaction=sear ch.jobDetails&template=dsp_job_details.cfm&cJobId=955099 Or contact Marilynn K. McCoy Employment Specialist Akron Children's Hospital One Perkins Square Akron, OH 44308-1062 330-543-3443 phone 330-543-3176 fax ------------------------------ Message: 13 Date: Thu, 19 Aug 2010 15:19:03 -0700 From: Maria Katleba Subject: RE: [Histonet] Corrective Action Form To: Rene J Buesa , "histonet@lists.utsouthwestern.edu" , Laurie Colbert Message-ID: Content-Type: text/plain; charset="iso-8859-1" You don't want to call it corrective action, as that indicates 'negligence' or 'failure to follow direction' on the part of the tech... Unless that is the case and you are looking to go after an employee...... Instead you need to call it Performance Improvement... This notes a problem, reason for the problem, and a solution to the problem... followed up by a time line where you follow the change in Policy and Procedure. It shows a problem that occurred where patient safety was compromised and a solution to the problem to avoid any potential future. You document this... Say you change the procedure today... well, keep track of the IHCs for the next 2 months...6 months (what ever timeline you choose)... then write up the conclusion to your findings. Here's and example: "The change to preparing IHC slides per Policy and Procedure #2001 on May 1, 2010 and studied for 4 months, we found no more issues regarding false positives." Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, August 19, 2010 12:47 PM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: Re: [Histonet] Corrective Action Form Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and they have to be approved by the HR Dept. Ren? J. --- On Thu, 8/19/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Corrective Action Form To: histonet@lists.utsouthwestern.edu Date: Thursday, August 19, 2010, 12:32 PM We recently had a false positive result on one of our immuno stains. I need to write up a report indicating the problem and what the corrective action was. Does anyone have a standard form that they use for Histology problems and resolutions that they would be willing to share with me? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ------------------------------ Message: 14 Date: Thu, 19 Aug 2010 17:04:57 -0700 (PDT) From: Kim Tournear Subject: Fw: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) To: Histonet Message-ID: <811192.15635.qm@web54207.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ~Kim~ ~Don't be afraid your life will?end, be afraid it will never begin~ OU Rocks!!!! --- On Thu, 8/19/10, Kim Tournear wrote: From: Kim Tournear Subject: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) To: "Sebree Linda A" Date: Thursday, August 19, 2010, 5:02 PM Bond Max or Bond III users: ? BD Biosciences (clone 24) 1:35 with?ER1 for 20 minutes (low pH) Biocare (BC/24) 1:50 with ER2 for 20 minutes (high pH) VBS (clone 1EW) 1:150 with ER2 for 25 minutes (high pH) VBS (clone 1EW) 1:40 with ER1 for 20 minutes (low pH) Biocare (clone BC/24) 1:50 with ER2 for 20 minutes (high pH) ? These protocols come from various sites using the Bond instrument.... Tonsil is the control of choice. ? Hope this helps..... ~Kim~ ~Don't be afraid your life will?end, be afraid it will never begin~ OU Rocks!!!! --- On Thu, 8/19/10, Sebree Linda A wrote: From: Sebree Linda A Subject: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) To: "Taylor, Jean" , histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com Date: Thursday, August 19, 2010, 7:57 AM Hi Jean, We use Cell Marque's (via VMS) clone 24.? We have to throw the entire arsenal at our disposal at these stains but they're acceptable. Good luck Jean; any questions, you know where we live! Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Thursday, August 19, 2010 9:45 AM To: 'histonet@lists.utsouthwestern.edu'; 'ihcrg@googlegroups.com' Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) Hi Everyone, I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP). Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 18, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to ? ? ? ? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ? ? ? ? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ? ? ? ? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ? ? ? ? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. RE: CD34 positive control (McMahon, Loralee A) ???2. Ventana Renaissance Containers (ricky hachy) ???3. CD34 Control (Silverman, Jeffrey) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger , Histonet ? ? ? ? Message-ID: Content-Type: text/plain; charset="us-ascii" Just about any tissue should work.???It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensperger@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control ? I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 18 Aug 2010 16:28:39 +0000 From: ricky hachy Subject: [Histonet] Ventana Renaissance Containers To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello , I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky ------------------------------ Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: "Silverman, Jeffrey" Subject: [Histonet] CD34 Control To: "'jreichensperger@siumed.edu'" Cc: "'histonet@lists.utsouthwestern.edu'" ? ? ? ? Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE41442A3@SYKECHXVS01.nslijhs.net> Content-Type: text/plain; charset="us-ascii" Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis,? are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 19 Aug 2010 19:12:38 -0700 From: Leroy Brown Subject: [Histonet] looking for any type of used paraffin block files To: Message-ID: Content-Type: text/plain; charset="iso-8859-1"; I am looking for paraffin block files. If you have some you no longer use or want I would be happy to talk to you. The files are for my personal use in my lab. Not for selling etc. Please, no equipment dealers or suppliers. I am looking for used. thanks for your help. LeRoy Brown HT(ASCP) HTL HCS, Inc. Everson, WA 98247 360-966-7300 ------------------------------ Message: 16 Date: Thu, 19 Aug 2010 22:02:37 -0600 From: Michael Folsom Subject: Re: [Histonet] Retracting microtomes To: "Sharon.Davis-Devine" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1282276957.3894.7.camel@chico.322tulane.org> Content-Type: text/plain; charset="UTF-8" Hi: Have you considered the possibility that there is something wrong with the microtome? Sadly equipment that is "inherited" can have issues. Perhaps a good cleaning is in order - By the way, what the make/model of the tome? I have a couple of B&L's that are available for a trade --;-) Later - Mike Rio Grande Biological Albuquerque, NM 87106 On Thu, 2010-08-19 at 11:10 -0500, Sharon.Davis-Devine wrote: > I have another question for all of you experts out there. We have a brand new microtome that we inherited from another laboratory. It is a retracting microtome whereas all of the other microtomes in our laboratory are not. None of our techs like this new microtome and find it difficult to use. My question to you as a group is, do many of you have retracting microtomes in your laboratory? If so, do you insist that techs in your laboratory learn to use this type of microtome even if they do not feel comfortable using it? > > The reason I ask is that I want to replace it with an older model which is non-retracting and management is insisting that since we already have it here our techs just need to learn how to use it. We have students rotating thru the rotations and often have to use different microtomes, I am worried that if we have one outlier microtome which operates differently specimens could be compromised. I welcome all your thoughts and opinions. > > Sharon Davis-Devine, CT (ASCP) > Cytology-Histology Supervisor > Carle Foundation Hospital > Laboratory and Pathology Services > 611 West Park Street > Urbana, Illinois 61801 > 217-383-3572 > sharon.davis-devine@carle.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Fri, 20 Aug 2010 09:12:56 +0000 From: joelle weaver Subject: RE: [Histonet] Corrective Action Form To: , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" How true, but a lot of people don't understand that and get it all wrong! The inspectors just want to see the identification of problems, the follow-up and the resolution. The feedback loop. -Joelle > From: lblazek@digestivespecialists.com > To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; laurie.colbert@huntingtonhospital.com > Date: Thu, 19 Aug 2010 16:02:46 -0400 > Subject: RE: [Histonet] Corrective Action Form > CC: > > Corrective actions don't have to lead to punishment! Corrective action should be a positive quality assurance. How can we make it better and not have this happen this way again type of thing. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, August 19, 2010 3:47 PM > To: histonet@lists.utsouthwestern.edu; Laurie Colbert > Subject: Re: [Histonet] Corrective Action Form > > Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and they have to be approved by the HR Dept. > Ren? J. > > --- On Thu, 8/19/10, Laurie Colbert wrote: > > > From: Laurie Colbert > Subject: [Histonet] Corrective Action Form > To: histonet@lists.utsouthwestern.edu > Date: Thursday, August 19, 2010, 12:32 PM > > > We recently had a false positive result on one of our immuno stains. I > need to write up a report indicating the problem and what the corrective > action was. Does anyone have a standard form that they use for > Histology problems and resolutions that they would be willing to share > with me? > > > > Thanks, > > Laurie Colbert > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Fri, 20 Aug 2010 08:07:30 -0400 From: "Blazek, Linda" Subject: [Histonet] job opening To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E390EAF72C73F@IBMB7Exchange.digestivespeci alists.com> Content-Type: text/plain; charset="us-ascii" We have a job opening for a full time days certified histotech in the Dayton, Ohio area. We have a great team and state of the art equipment. Please feel free to contact me for more information. Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com ------------------------------ Message: 19 Date: Fri, 20 Aug 2010 08:33:31 -0400 From: "Houston, Ronald" Subject: [Histonet] cpt code for fused twin placentas To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Can you charge 88307 x2 for fused twin placentas? I'm not sure as strictly only one specimen was received, but our billing company says yes? Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 20 Date: Fri, 20 Aug 2010 10:12:08 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: cpt code for fused twin placentas To: "Houston, Ronald" , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164033215FD25@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Yes, if the pathologist is giving two separate diagnoses. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, August 20, 2010 08:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cpt code for fused twin placentas Can you charge 88307 x2 for fused twin placentas? I'm not sure as strictly only one specimen was received, but our billing company says yes? Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 21 Date: Fri, 20 Aug 2010 07:36:58 -0700 From: Subject: [Histonet] Hawaii To: histonet@lists.utsouthwestern.edu Message-ID: <20100820073658.9e2d9aa830e8449a2412eb1e4f2f067e.58c9ef4452.wbe@email04. secureserver.net> Content-Type: text/plain; charset="utf-8" Does anyone know of any open positions in Hawaii, and what is the expected payscale there? I have a BA, HT, and a very good knowledge of IHC. I have 6 years registered experience with 2 years histo. lab nd my fiance might be moving there because of his job. Any help would Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 [DEL: (5 :DEL] ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 26 **************************************** From mmashore <@t> vapop.ucsd.edu Fri Aug 20 14:15:28 2010 From: mmashore <@t> vapop.ucsd.edu (Michael Mashore) Date: Fri Aug 20 14:19:50 2010 Subject: [Histonet] protocol for Masson-Goldner trichrome modified by Ulrich Message-ID: <616D0C3F5CEB486F8AC342F3277C716C@vandeberg> Hello everyone, I'm trying to use the Masson-Goldner Trichrome stain, and I came across a paper that used one that was modified by Ulrich. There was no citation on the paper, and I wanted more details to their protocol, i.e. how they made their solutions. The paper is titled "Histology and research at the hard tissue-implant interface using Technovit 9100 New embedding technique" by Elmar Willbold and Frank Witt. Does anyone know more details on this stain? We are using it on hard tissue embedding, where we used methylmethacrylate. Thanks for the help! From rags_jeg <@t> yahoo.co.in Fri Aug 20 15:19:03 2010 From: rags_jeg <@t> yahoo.co.in (vaigunda ragavendran) Date: Fri Aug 20 15:19:09 2010 Subject: [Histonet] Re: Cryoprotection Message-ID: <160206.43907.qm@web8701.mail.in.yahoo.com> Hii, If instead of sucrose in PBS, we use sucrose in DW by mistake, what might happen to fixed tissue sections during cryoprotection.. Please lemme know.. Rags, Postdoctoral Research Fellow, Verge Lab, Department of Anatomy and Cell Biology, Room No: 5800, Saskatoon City Hospital, 701, Queen Street, Saskatoon, Saskatchewan S7K 0M7, Canada. Phone (Lab): +1 306-655-8714 ????????? (Facsimile): +1 306-655-8709 From garret.t.miyamoto <@t> us.army.mil Fri Aug 20 15:34:11 2010 From: garret.t.miyamoto <@t> us.army.mil (Miyamoto, Garret T Mr CIV USA USAMEDCOM) Date: Fri Aug 20 15:34:20 2010 Subject: [Histonet] Re: Hawaii In-Reply-To: <0L7G00D4SNQPZ1H0@mail23.us.army.mil> References: <0L7G00D4SNQPZ1H0@mail23.us.army.mil> Message-ID: Sarah, We had a position open at Tripler Army Medical Center but unfortunately it closed yesterday. I would suggest you try "Queens Hospital", "Clinical Labs of Hawaii", "Kuakini Medical Center", and "Kaiser Medical Center". They should have websites and you can probably inquire through them. Hope things will work out for you. Garret Miyamoto Tripler Army Medical Center Anatomic Pathology Lab ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Friday, August 20, 2010 7:11 am Subject: Histonet Digest, Vol 81, Issue 26 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator > protein (BSAP) (Taylor, Jean) > 2. [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator > protein (BSAP) (Taylor, Jean) > 3. Staining Question (Herrick, James L. (Jim)) > 4. Re: Staining Question (Jack Ratliff) > 5. [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator > protein (BSAP) (Taylor, Jean) > 6. Re: Corrective Action Form (Rene J Buesa) > 7. Gram Control (Mark Tarango) > 8. RE: Corrective Action Form (Blazek, Linda) > 9. RE: Corrective Action Form (Laurie Colbert) > 10. Re: Corrective Action Form (Akemi Allison) > 11. Adenovirus controls (Joe Nocito) > 12. Job posting for Akron Children's Hospital (McCoy, Marilynn) > 13. RE: Corrective Action Form (Maria Katleba) > 14. Fw: RE: [Histonet] PAX-5 - B-cell specific activator protein > (BSAP) (Kim Tournear) > 15. looking for any type of used paraffin block files (Leroy Brown) > 16. Re: Retracting microtomes (Michael Folsom) > 17. RE: Corrective Action Form (joelle weaver) > 18. job opening (Blazek, Linda) > 19. cpt code for fused twin placentas (Houston, Ronald) > 20. RE: cpt code for fused twin placentas (Weems, Joyce) > 21. Hawaii (sgoebel@xbiotech.com) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Fri, 20 Aug 2010 03:25:41 +1000 > From: "Taylor, Jean" < > Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator > protein (BSAP) > To: "Sebree Linda A" < > Cc: histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com > Message-ID: < > Content-Type: text/plain; boundary="1282238741.aC6ACbF0.3413"; > charset="us-ascii" > > > Dear Sir/Madam, > Your email was unable reach the intended person that you were sending > it to. > For more information on our business please click on the following > link: > [1]Click here for our website > We look forward to your continued business in the future. > Regards, > Webmaster > > References > > 1. http://www.downwind.com.au/avdir/rd.php?id=7564 > > > ------------------------------ > > Message: 2 > Date: Fri, 20 Aug 2010 04:01:22 +1000 > From: "Taylor, Jean" < > Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator > protein (BSAP) > To: "Sebree Linda A" < > Cc: histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com > Message-ID: < > Content-Type: text/plain; boundary="1282240882.203a0.15929"; > charset="us-ascii" > > > Dear Sir/Madam, > Your email was unable reach the intended person that you were sending > it to. > For more information on our business please click on the following > link: > [1]Click here for our website > We look forward to your continued business in the future. > Regards, > Webmaster > > References > > 1. http://www.downwind.com.au/avdir/rd.php?id=7564 > > > ------------------------------ > > Message: 3 > Date: Thu, 19 Aug 2010 14:22:33 -0500 > From: "Herrick, James L. (Jim)" < > Subject: [Histonet] Staining Question > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Good afternoon everybody, > > Would anyone be willing to offer their wisdom on the best etching > solution for 50 to 75 micron thick jaw specimens embedded in MMA? The > stain we want to use is a Trichrome. Thanks in advance for your help. > > Jim > > > > > ------------------------------ > > Message: 4 > Date: Thu, 19 Aug 2010 14:28:10 -0500 > From: Jack Ratliff < > Subject: Re: [Histonet] Staining Question > To: "Herrick, James L. (Jim)" < > Cc: "<" > < > Message-ID: < > Content-Type: text/plain; charset=us-ascii > > 0.7% Formic Acid for 30 seconds with sonication, then rinse well in DI water. > > Jack > > On Aug 19, 2010, at 2:22 PM, "Herrick, James L. (Jim)" < wrote: > > > Good afternoon everybody, > > > > Would anyone be willing to offer their wisdom on the best etching > > solution for 50 to 75 micron thick jaw specimens embedded in MMA? The > > stain we want to use is a Trichrome. Thanks in advance for your help. > > > > Jim > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 5 > Date: Fri, 20 Aug 2010 05:39:52 +1000 > From: "Taylor, Jean" < > Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator > protein (BSAP) > To: "Sebree Linda A" < > Cc: histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com > Message-ID: < > Content-Type: text/plain; boundary="1282246792.5b22a0.23037"; > charset="us-ascii" > > > Dear Sir/Madam, > Your email was unable reach the intended person that you were sending > it to. > For more information on our business please click on the following > link: > [1]Click here for our website > We look forward to your continued business in the future. > Regards, > Webmaster > > References > > 1. http://www.downwind.com.au/avdir/rd.php?id=7564 > > > ------------------------------ > > Message: 6 > Date: Thu, 19 Aug 2010 12:47:28 -0700 (PDT) > From: Rene J Buesa < > Subject: Re: [Histonet] Corrective Action Form > To: histonet@lists.utsouthwestern.edu, Laurie Colbert > < > Message-ID: < > Content-Type: text/plain; charset=iso-8859-1 > > Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and?they have to be approved by the HR?Dept. > Ren? J.? > > --- On Thu, 8/19/10, Laurie Colbert < wrote: > > > From: Laurie Colbert < > Subject: [Histonet] Corrective Action Form > To: histonet@lists.utsouthwestern.edu > Date: Thursday, August 19, 2010, 12:32 PM > > > We recently had a false positive result on one of our immuno stains.? I > need to write up a report indicating the problem and what the corrective > action was.? Does anyone have a standard form that they use for > Histology problems and resolutions that they would be willing to share > with me? > > > > Thanks, > > Laurie Colbert > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 7 > Date: Thu, 19 Aug 2010 13:01:05 -0700 > From: Mark Tarango < > Subject: [Histonet] Gram Control > To: "histonet@lists.utsouthwestern.edu" > < > Message-ID: > < > Content-Type: text/plain; charset=ISO-8859-1 > > Would anyone want to trade for a gram control? I have lots of H. pylori > control blocks made up. I could trade for other things too... > > Thanks! > Mark > > > ------------------------------ > > Message: 8 > Date: Thu, 19 Aug 2010 16:02:46 -0400 > From: "Blazek, Linda" < > Subject: RE: [Histonet] Corrective Action Form > To: 'Rene J Buesa' <, > "histonet@lists.utsouthwestern.edu" > <, Laurie Colbert > < > Message-ID: > < > > Content-Type: text/plain; charset="iso-8859-1" > > Corrective actions don't have to lead to punishment! Corrective action should be a positive quality assurance. How can we make it better and not have this happen this way again type of thing. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, August 19, 2010 3:47 PM > To: histonet@lists.utsouthwestern.edu; Laurie Colbert > Subject: Re: [Histonet] Corrective Action Form > > Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and?they have to be approved by the HR?Dept. > Ren? J.? > > --- On Thu, 8/19/10, Laurie Colbert < wrote: > > > From: Laurie Colbert < > Subject: [Histonet] Corrective Action Form > To: histonet@lists.utsouthwestern.edu > Date: Thursday, August 19, 2010, 12:32 PM > > > We recently had a false positive result on one of our immuno stains.? I > need to write up a report indicating the problem and what the corrective > action was.? Does anyone have a standard form that they use for > Histology problems and resolutions that they would be willing to share > with me? > > > > Thanks, > > Laurie Colbert > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Thu, 19 Aug 2010 14:26:26 -0700 > From: "Laurie Colbert" < > Subject: RE: [Histonet] Corrective Action Form > To: "Rene J Buesa" <, > < > Message-ID: > < > > Content-Type: text/plain; charset="iso-8859-1" > > I'm looking at it from the standpoint of an inspector - we had a problem and what steps did we follow to correct it and prevent it from happening again. I'm not planning on using it as a punishment for anyone. > > > > ________________________________ > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, August 19, 2010 12:47 PM > To: histonet@lists.utsouthwestern.edu; Laurie Colbert > Subject: Re: [Histonet] Corrective Action Form > > > > Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and they have to be approved by the HR Dept. > > Ren? J. > > --- On Thu, 8/19/10, Laurie Colbert < wrote: > > > From: Laurie Colbert < > Subject: [Histonet] Corrective Action Form > To: histonet@lists.utsouthwestern.edu > Date: Thursday, August 19, 2010, 12:32 PM > > We recently had a false positive result on one of our immuno stains. I > need to write up a report indicating the problem and what the corrective > action was. Does anyone have a standard form that they use for > Histology problems and resolutions that they would be willing to share > with me? > > > > Thanks, > > Laurie Colbert > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 10 > Date: Thu, 19 Aug 2010 14:55:40 -0700 > From: Akemi Allison < > Subject: Re: [Histonet] Corrective Action Form > To: "Laurie Colbert" < > Cc: histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed > > This is used in concordance to CAP guidelines for PIP. The phrasing > scares people off because ii states "Incident/Complaint Form". > People need to realize we ALL make mistakes. This form is used for > corrective measures and process improvement in Pre-Analytical- > Technical-Analytical & Post-Analytical. It is recommended to have > Quarterly Reviews by Committee members to address what Corrective > Measures have been implemented. > > Remember folks, this is for improving patient care and not to finger > point or put blame on anyone. > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > On Aug 19, 2010, at 2:26 PM, Laurie Colbert wrote: > > > I'm looking at it from the standpoint of an inspector - we had a > > problem and what steps did we follow to correct it and prevent it > > from happening again. I'm not planning on using it as a punishment > > for anyone. > > > > > > > > ________________________________ > > > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > > Sent: Thursday, August 19, 2010 12:47 PM > > To: histonet@lists.utsouthwestern.edu; Laurie Colbert > > Subject: Re: [Histonet] Corrective Action Form > > > > > > > > Probably many of us have but it would be better if you consult with > > your HR Dept. because corrective actions could lead to some type of > > "punishment" and they have to be approved by the HR Dept. > > > > Ren? J. > > > > --- On Thu, 8/19/10, Laurie Colbert > > < wrote: > > > > > > From: Laurie Colbert < > > Subject: [Histonet] Corrective Action Form > > To: histonet@lists.utsouthwestern.edu > > Date: Thursday, August 19, 2010, 12:32 PM > > > > We recently had a false positive result on one of our immuno > > stains. I > > need to write up a report indicating the problem and what the > > corrective > > action was. Does anyone have a standard form that they use for > > Histology problems and resolutions that they would be willing to > > share > > with me? > > > > > > > > Thanks, > > > > Laurie Colbert > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > mc/compose?to=Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 11 > Date: Thu, 19 Aug 2010 16:59:05 -0500 > From: "Joe Nocito" < > Subject: [Histonet] Adenovirus controls > To: "Histonet" < > Message-ID: < > Content-Type: text/plain; charset="iso-8859-1" > > Greetings histoland, > does any one know of a company that sells adenovirus controls slides? I've tried NewComer's Supply, MasterTech, Biocare, Biogenex, ThermoFisherRichard Allen, or whatever they're called this week. Thanks > > Joe > > > ------------------------------ > > Message: 12 > Date: Thu, 19 Aug 2010 18:05:25 -0400 > From: "McCoy, Marilynn" < > Subject: [Histonet] Job posting for Akron Children's Hospital > To: "histonet@lists.utsouthwestern.edu" > < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > Would you like an opportunity to use your versatile skills in a great lab environment? Akron Children's Hospital, located in Northeast Ohio, has a part-time opening for a Histology Technician - 20 hours per week, variable, day shift. > > Qualifications: Preferred - Associate degree in Histology with certification or eligibility for certification by ASCP or minimum of three years previous experience as a Histotech. Also Considered - Associate degree in Medical Laboratory Technology with certification or a BS or Masters in a scientific area such as Medical Technology and ability to learn and perform histology/Diener skills. Histology/Electron Microscopy and/or Diener experience preferred. > > Check out the opportunity at https://www.healthcaresource.com/akronchildren/index.cfm?fuseaction=search.jobDetails&template=dsp_job_details.cfm&cJobId=955099 > > Or contact Marilynn K. McCoy Employment Specialist > Akron Children's Hospital > One Perkins Square > Akron, OH 44308-1062 > 330-543-3443 phone > 330-543-3176 fax > > > > ------------------------------ > > Message: 13 > Date: Thu, 19 Aug 2010 15:19:03 -0700 > From: Maria Katleba < > Subject: RE: [Histonet] Corrective Action Form > To: Rene J Buesa <, > "histonet@lists.utsouthwestern.edu" > <, Laurie Colbert > < > Message-ID: > < > Content-Type: text/plain; charset="iso-8859-1" > > You don't want to call it corrective action, as that indicates 'negligence' or 'failure to follow direction' on the part of the tech... Unless that is the case and you are looking to go after an employee...... > > Instead you need to call it Performance Improvement... This notes a problem, reason for the problem, and a solution to the problem... followed up by a time line where you follow the change in Policy and Procedure. > > It shows a problem that occurred where patient safety was compromised and a solution to the problem to avoid any potential future. > > You document this... Say you change the procedure today... well, keep track of the IHCs for the next 2 months...6 months (what ever timeline you choose)... then write up the conclusion to your findings. > > Here's and example: "The change to preparing IHC slides per Policy and Procedure #2001 on May 1, 2010 and studied for 4 months, we found no more issues regarding false positives." > > > > Maria Katleba MS HT(ASCP) > Pathology Dept. Mgr > Queen of the Valley Medical Center > 1000 Trancas Street > Napa CA 94558 > (707) 252-4411 x3689 direct > (707) 226-4385 pager > (707) 294-9229 cell- anytime > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, August 19, 2010 12:47 PM > To: histonet@lists.utsouthwestern.edu; Laurie Colbert > Subject: Re: [Histonet] Corrective Action Form > > Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and they have to be approved by the HR Dept. > Ren? J. > > --- On Thu, 8/19/10, Laurie Colbert < wrote: > > > From: Laurie Colbert < > Subject: [Histonet] Corrective Action Form > To: histonet@lists.utsouthwestern.edu > Date: Thursday, August 19, 2010, 12:32 PM > > > We recently had a false positive result on one of our immuno stains. I > need to write up a report indicating the problem and what the corrective > action was. Does anyone have a standard form that they use for > Histology problems and resolutions that they would be willing to share > with me? > > > > Thanks, > > Laurie Colbert > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Notice from St. Joseph Health System: > Please note that the information contained in this message may be privileged and confidential and protected from disclosure. > > > > > ------------------------------ > > Message: 14 > Date: Thu, 19 Aug 2010 17:04:57 -0700 (PDT) > From: Kim Tournear < > Subject: Fw: RE: [Histonet] PAX-5 - B-cell specific activator protein > (BSAP) > To: Histonet < > Message-ID: < > Content-Type: text/plain; charset=iso-8859-1 > > > > > > ~Kim~ > ~Don't be afraid your life will?end, > be afraid it will never begin~ > OU Rocks!!!! > > --- On Thu, 8/19/10, Kim Tournear < wrote: > > > From: Kim Tournear < > Subject: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) > To: "Sebree Linda A" < > Date: Thursday, August 19, 2010, 5:02 PM > > > > > > > > Bond Max or Bond III users: > ? > BD Biosciences (clone 24) 1:35 with?ER1 for 20 minutes (low pH) > Biocare (BC/24) 1:50 with ER2 for 20 minutes (high pH) > VBS (clone 1EW) 1:150 with ER2 for 25 minutes (high pH) > VBS (clone 1EW) 1:40 with ER1 for 20 minutes (low pH) > Biocare (clone BC/24) 1:50 with ER2 for 20 minutes (high pH) > ? > These protocols come from various sites using the Bond instrument.... > Tonsil is the control of choice. > ? > Hope this helps..... > > > ~Kim~ > ~Don't be afraid your life will?end, > be afraid it will never begin~ > OU Rocks!!!! > > --- On Thu, 8/19/10, Sebree Linda A < wrote: > > > From: Sebree Linda A < > Subject: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) > To: "Taylor, Jean" <, histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com > Date: Thursday, August 19, 2010, 7:57 AM > > > Hi Jean, > > We use Cell Marque's (via VMS) clone 24.? We have to throw the entire > arsenal at our disposal at these stains but they're acceptable. > > Good luck Jean; any questions, you know where we live! > > Linda > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, > Jean > Sent: Thursday, August 19, 2010 9:45 AM > To: 'histonet@lists.utsouthwestern.edu'; 'ihcrg@googlegroups.com' > Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) > > Hi Everyone, > > I'm trying to find out what company and clone most labs are using for > PAX-5 (BSAP). > > Thanks! > > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histonet-request@lists.utsouthwestern.edu > Sent: Wednesday, August 18, 2010 12:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 81, Issue 23 > > Send Histonet mailing list submissions to > ? ? ? ? histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ? http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ? histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ? histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than > "Re: Contents of Histonet digest..." > > > Today's Topics: > > ???1. RE: CD34 positive control (McMahon, Loralee A) > ???2. Ventana Renaissance Containers (ricky hachy) > ???3. CD34 Control (Silverman, Jeffrey) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 18 Aug 2010 12:17:39 -0400 > From: "McMahon, Loralee A" < > Subject: RE: [Histonet] CD34 positive control > To: Joel Reichensperger <, Histonet > ? ? ? ? < > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > Just about any tissue should work.???It stains for Hematopoietic > stem/progenitor cells, bone marrow stromal cells, endothelial cells, > embryonic fibroblasts > We use a sausage style control here with about 30 different types of > tissue. > > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel > Reichensperger [jreichensperger@siumed.edu] > Sent: Wednesday, August 18, 2010 12:05 PM > To: Histonet > Subject: [Histonet] CD34 positive control > > ? I have a doctor who wants to stain some tissue with cd34. I need to > know if anyone can recommend a good positive control tissue for this > antibody. The staining will be done in paraffin embedded sections. > Thanks in advance. > > Joel > > -- > Joel Reichensperger > Researcher II > Southern Illinois University > Plastic Surgery Institute > jreichensperger@siumed.edu > 217-545-7309 (Office) > 217-545-1824 (Fax) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 2 > Date: Wed, 18 Aug 2010 16:28:39 +0000 > From: ricky hachy < > Subject: [Histonet] Ventana Renaissance Containers > To: Histonet < > Message-ID: < > Content-Type: text/plain; charset="iso-8859-1" > > > Hello , > > I need 4 good plastic containers, where you fill with > xilene,alcool....... for the VENTANA RENAISSANCE . > > Anyone could sell me ? > > Regards > > Ricky > > ------------------------------ > > Message: 3 > Date: Wed, 18 Aug 2010 12:30:58 -0400 > From: "Silverman, Jeffrey" < > Subject: [Histonet] CD34 Control > To: "'jreichensperger@siumed.edu'" < > Cc: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? < > Message-ID: > > < > Content-Type: text/plain; charset="us-ascii" > > Joel, > A section of skin will be a fine CD34 control. All collagenous > connective tissue, including the dermis,? are rich in CD34+ dendritic > interstitial fibroblasts as well as CD34 positive endothelium in the > resident vessels. Actually, in my lab we use a section of fallopian tube > from tubal ligation, they are loaded with the fibroblasts and vessels. > > Jeff Silverman > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 81, Issue 23 > **************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 15 > Date: Thu, 19 Aug 2010 19:12:38 -0700 > From: Leroy Brown < > Subject: [Histonet] looking for any type of used paraffin block files > To: < > Message-ID: > < > Content-Type: text/plain; charset="iso-8859-1"; > > I am looking for paraffin block files. If you have some you no longer use or want I would be happy to talk to you. The files are for my personal use in my lab. Not for selling etc. Please, no equipment dealers or suppliers. I am looking for used. > > thanks for your help. > > LeRoy Brown HT(ASCP) HTL > > HCS, Inc. > > Everson, WA 98247 > > 360-966-7300 > > > ------------------------------ > > Message: 16 > Date: Thu, 19 Aug 2010 22:02:37 -0600 > From: Michael Folsom < > Subject: Re: [Histonet] Retracting microtomes > To: "Sharon.Davis-Devine" < > Cc: "histonet@lists.utsouthwestern.edu" > < > Message-ID: < > Content-Type: text/plain; charset="UTF-8" > > Hi: > > Have you considered the possibility that there is something wrong with > the microtome? Sadly equipment that is "inherited" can have issues. > > Perhaps a good cleaning is in order - > > By the way, what the make/model of the tome? I have a couple of B&L's > that are available for a trade --;-) > > > Later - > > Mike > Rio Grande Biological > Albuquerque, NM 87106 > > > On Thu, 2010-08-19 at 11:10 -0500, Sharon.Davis-Devine wrote: > > I have another question for all of you experts out there. We have a brand new microtome that we inherited from another laboratory. It is a retracting microtome whereas all of the other microtomes in our laboratory are not. None of our techs like this new microtome and find it difficult to use. My question to you as a group is, do many of you have retracting microtomes in your laboratory? If so, do you insist that techs in your laboratory learn to use this type of microtome even if they do not feel comfortable using it? > > > > The reason I ask is that I want to replace it with an older model which is non-retracting and management is insisting that since we already have it here our techs just need to learn how to use it. We have students rotating thru the rotations and often have to use different microtomes, I am worried that if we have one outlier microtome which operates differently specimens could be compromised. I welcome all your thoughts and opinions. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology-Histology Supervisor > > Carle Foundation Hospital > > Laboratory and Pathology Services > > 611 West Park Street > > Urbana, Illinois 61801 > > 217-383-3572 > > sharon.davis-devine@carle.com > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 17 > Date: Fri, 20 Aug 2010 09:12:56 +0000 > From: joelle weaver < > Subject: RE: [Histonet] Corrective Action Form > To: <, Histonet > < > Message-ID: < > Content-Type: text/plain; charset="iso-8859-1" > > > How true, but a lot of people don't understand that and get it all wrong! The inspectors just want to see the identification of problems, the follow-up and the resolution. The feedback loop. > -Joelle > > > From: lblazek@digestivespecialists.com > > To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; laurie.colbert@huntingtonhospital.com > > Date: Thu, 19 Aug 2010 16:02:46 -0400 > > Subject: RE: [Histonet] Corrective Action Form > > CC: > > > > Corrective actions don't have to lead to punishment! Corrective action should be a positive quality assurance. How can we make it better and not have this happen this way again type of thing. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > > Sent: Thursday, August 19, 2010 3:47 PM > > To: histonet@lists.utsouthwestern.edu; Laurie Colbert > > Subject: Re: [Histonet] Corrective Action Form > > > > Probably many of us have but it would be better if you consult with your HR Dept. because corrective actions could lead to some type of "punishment" and they have to be approved by the HR Dept. > > Ren? J. > > > > --- On Thu, 8/19/10, Laurie Colbert < wrote: > > > > > > From: Laurie Colbert < > > Subject: [Histonet] Corrective Action Form > > To: histonet@lists.utsouthwestern.edu > > Date: Thursday, August 19, 2010, 12:32 PM > > > > > > We recently had a false positive result on one of our immuno stains. I > > need to write up a report indicating the problem and what the corrective > > action was. Does anyone have a standard form that they use for > > Histology problems and resolutions that they would be willing to share > > with me? > > > > > > > > Thanks, > > > > Laurie Colbert > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 18 > Date: Fri, 20 Aug 2010 08:07:30 -0400 > From: "Blazek, Linda" < > Subject: [Histonet] job opening > To: "histonet@lists.utsouthwestern.edu" > < > Message-ID: > < > > Content-Type: text/plain; charset="us-ascii" > > We have a job opening for a full time days certified histotech in the Dayton, Ohio area. We have a great team and state of the art equipment. > Please feel free to contact me for more information. > > > Our Vision: To be the #1 choice for all your GI services > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Digestive Specialists, Inc > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > > > ------------------------------ > > Message: 19 > Date: Fri, 20 Aug 2010 08:33:31 -0400 > From: "Houston, Ronald" < > Subject: [Histonet] cpt code for fused twin placentas > To: "histonet@lists.utsouthwestern.edu" > < > Message-ID: > < > > Content-Type: text/plain; charset="us-ascii" > > Can you charge 88307 x2 for fused twin placentas? I'm not sure as strictly only one specimen was received, but our billing company says yes? > > > Ronnie Houston, MS HT(ASCP)QIHC > Anatomic Pathology Manager > > ChildLab, a Division of Nationwide Children's Hospital > > www.childlab.com > > > 700 Children's Drive > Columbus, OH 43205 > (P) 614-722-5450 > (F) 614-722-2899 > ronald.houston@nationwidechildrens.org > www.NationwideChildrens.org > > "One person with passion is better than forty people merely interested." > ~ E.M. Forster > > > > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > > ------------------------------ > > Message: 20 > Date: Fri, 20 Aug 2010 10:12:08 -0400 > From: "Weems, Joyce" < > Subject: [Histonet] RE: cpt code for fused twin placentas > To: "Houston, Ronald" <, > "histonet@lists.utsouthwestern.edu" > < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > Yes, if the pathologist is giving two separate diagnoses. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald > Sent: Friday, August 20, 2010 08:34 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cpt code for fused twin placentas > > Can you charge 88307 x2 for fused twin placentas? I'm not sure as strictly only one specimen was received, but our billing company says yes? > > > Ronnie Houston, MS HT(ASCP)QIHC > Anatomic Pathology Manager > > ChildLab, a Division of Nationwide Children's Hospital > > www.childlab.com > > > 700 Children's Drive > Columbus, OH 43205 > (P) 614-722-5450 > (F) 614-722-2899 > ronald.houston@nationwidechildrens.org > www.NationwideChildrens.org > > "One person with passion is better than forty people merely interested." > ~ E.M. Forster > > > > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > > > > ------------------------------ > > Message: 21 > Date: Fri, 20 Aug 2010 07:36:58 -0700 > From: < > Subject: [Histonet] Hawaii > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > > Content-Type: text/plain; charset="utf-8" > > > Does anyone know of any open positions in Hawaii, and what is the expected payscale there? I have a BA, HT, and a very good knowledge of IHC. I have 6 years registered experience with 2 years histo. lab > nd my fiance might be moving there because of his job. Any help > would > Sarah Goebel, B.A., HT (ASCP) > > Histotechnician > > XBiotech USA Inc. > > 8201 East Riverside Dr. Bldg 4 Suite 100 > > Austin, Texas 78744 > [DEL: (5 :DEL] > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 81, Issue 26 > **************************************** From sgoebel <@t> xbiotech.com Fri Aug 20 15:36:17 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Aug 20 15:36:21 2010 Subject: [Histonet] Re: Cryoprotection Message-ID: <20100820133617.9e2d9aa830e8449a2412eb1e4f2f067e.010f06a8ff.wbe@email04.secureserver.net> The cells could lyse Sarah Goebel, B.A., HT (ASCP) H XBiotech USA Inc. 8201 East Ri -------- Original Message -------- Subject: [Histonet] Re: Cryoprotection From: vaigunda ragavendran <[1]rags Date: Fri, August 20, 2010 1:19 pm To: [2]histonet@lists.uts Hii, If instead of sucrose in PBS, we use sucrose in DW by mistake, what might h Please lemme know.. Rags, Postdoctoral Research Fellow, Verge Lab, Department of Anatomy and Cell Biology, Room No: 5800, Saskatoon City Hospital, 701, Queen Street, Saskatoon, Saskatchewan S7K 0M7, Canada. Phone (Lab): +1 306-655-8714 (Facsimile): +1 306- _______________________________________________ Histonet mailing list [3]Histonet@lists.utsouth [4]http: References 1. 3D"mailto:rags_jeg@yahoo.co.in" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From afimbres <@t> uci.edu Fri Aug 20 16:17:46 2010 From: afimbres <@t> uci.edu (Fimbres, Amber) Date: Fri Aug 20 16:17:51 2010 Subject: [Histonet] Tissue-Tek VIP 2000-3000 Message-ID: Mike, I know I'm a little late regarding your question about Sakura's VIP 2000/3000. I'm not sure if you found your answer, but if you're talking about the VIP processor that uses a magnet to program, change stations, etc. (and is reddish orange in color and may even say 'Miles' on it instead of Sakura) you will want to think twice before purchasing it. These VIP K (series 1000, 2000, 3000) are no longer serviced by Sakura nor are there many parts for them. Sakura's manufacturer for parts has stopped making replaceable parts for this particular model (they are at least 20 or more years old). You can go to Sakura's website or call their technical support, they will confirm this too. Take care, Amber ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. From saby_joseph_a <@t> yahoo.com Fri Aug 20 16:45:21 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Fri Aug 20 16:45:28 2010 Subject: [Histonet] Tissue-Tek VIP 2000-3000 In-Reply-To: References: Message-ID: <56366.46162.qm@web114415.mail.gq1.yahoo.com> Mike- Although Sakura is no longer making parts for the VIP 2000 units, there are thousands still in use around the country.? Most companies that service these units have been taking them in trade as people upgrade their equipment, then using these units to provide parts for units in trouble along the way.? They are very dependable (with regular water rinses and periodic PM). Sakura does still makes parts for and service the VIP E300s, which is the step up from the 2000.? If the price were right, and funds were low, I would buy either. I have been using these units for many years. Joe Saby, BA HT ________________________________ From: "Fimbres, Amber" To: "histonet@lists.utsouthwestern.edu" Sent: Fri, August 20, 2010 5:17:46 PM Subject: [Histonet] Tissue-Tek VIP 2000-3000 Mike, I know I'm a little late regarding your question about Sakura's VIP 2000/3000.? I'm not sure if you found your answer, but if you're talking about the VIP processor that uses a magnet to program, change stations, etc. (and is reddish orange in color and may even say 'Miles' on it instead of Sakura) you will want to think twice before purchasing it.? These VIP K (series 1000, 2000, 3000) are no longer serviced by Sakura nor are there many parts for them.? Sakura's manufacturer for parts has stopped making replaceable parts for this particular model (they are at least 20 or more years old).? You can go to Sakura's website or call their technical support, they will confirm this too. Take care, Amber ? ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JefThompson <@t> salud.unm.edu Fri Aug 20 16:55:11 2010 From: JefThompson <@t> salud.unm.edu (Jeffrey Thompson) Date: Fri Aug 20 16:55:17 2010 Subject: [Histonet] cryostat Message-ID: <4C6EA55F0200004D000CEE26@hsc-iagate1.health.unm.edu> Hello, I am writing to ask about what the community views are on the best -or worst - cryostats. We may have the opportunity to purchase one for the first time in many years and we would like to get a good, solid, reliable model. We're not really looking for one (and can't really afford one) with all the bells and whistles, but would like to know what folks think about the ones to look for as well as the ones to steer clear of. All input is appreciated. Thanks, Jeff Thompson From sgoebel <@t> xbiotech.com Fri Aug 20 17:08:38 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Aug 20 17:08:43 2010 Subject: [Histonet] Tissue-Tek VIP 2000-3000 Message-ID: <20100820150838.9e2d9aa830e8449a2412eb1e4f2f067e.a1ad93de3e.wbe@email04.secureserver.net> Which I did buy it, and it had an issue, repair guy came wi days and fixed it with no problems. There are also other compa that are making parts for them that aren't "Sakura" but still work.&nb was the right pr Sarah Goebel, B.A., HT (ASCP) Histotechnician < XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 1 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: Re: [Histonet] Tissue-Tek VIP 2000-3000 From: Joseph Saby <[1]saby_josep Date: Fri, August 20, 2010 2:45 pm To: "Fimbres, Amber" <[2]afimbres@uci.e "[3]histonet@lists.utsout histonet@lists.utsouthwestern.edu> Mike- Although Sakura is no longer making parts for the VIP 2000 units, there are thousands still in use around the country. Most companies that servic units have been taking them in trade as people upgrade their equipment, the using these units to provide parts for units in trouble along the way. very dependable (with regular water rinses and periodic PM). Sakura does still makes parts for and service the VIP E300s, which is the s up from the 2000. If the price were right, and funds were low, I woul either. I have been using these units for many years. Joe Saby, BA HT ________________________________ From: "Fimbres, Amber" <[5]afimbres@uci To: "[6]histonet@lists.ut <[7]histonet@lists.utsouthwestern.edu> Sent: Fri, August 20, 2010 5:17:46 PM Subject: [Histonet] Tissue-Tek VIP 2000-3000 Mike, I know I'm a little late regarding your question about Sakura's VIP 2000/30 I'm not sure if you found your answer, but if you're talking about the VIP processor that uses a magnet to program, change stations, etc. (and is redd orange in color and may even say 'Miles' on it instead of Sakura) you will to think twice before purchasing it. These VIP K (series 1000, 2000, no longer serviced by Sakura nor are there many parts for them. Sakur manufacturer for parts has stopped making replaceable parts for this partic model (they are at least 20 or more years old). You can go to Sakura' or call their technical support, they will confirm this too. Take care, Amber ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not dissemi distribute or copy this e-mail. Please notify the sender immediately by e-m if you have received this e-mail by mistake and delete this e-mail from you system. E-mail transmission cannot be guaranteed to be secure or error-free information could be intercepted, corrupted, lost, destroyed, arrive late o incomplete, or contain viruses. The sender therefore does not accept liabil for any errors or omissions in the contents of this message, which arise as result of e-mail transmission. _______________________________________________ Histonet mailing list [8]Histonet@lists.utsouth [9]http: _______________________________________________ Histonet mailing list [10]Histonet@lists.utsouth [11]http: References 1. 3D"mailto:saby_joseph_a@yahoo.com" 2. 3D"mailto:afimbres@uci.edu" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet@lists.utsouthwestern.edu" 5. 3D"mailto:afimbres@uci.edu" 6. 3D"mailto:histonet@lists.utsouthwestern.edu" 7. 3D"mailto:histonet@lists.utsouthwestern.e 8. 3D"mailto:Histonet@lists.utsouthwestern.edu" 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 10. 3D"mailto:Histonet@lists.utsouthwestern.edu" 11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From NKlemme <@t> sakuraus.com Fri Aug 20 17:31:32 2010 From: NKlemme <@t> sakuraus.com (Nancy Klemme) Date: Fri Aug 20 17:32:34 2010 Subject: [Histonet] Tissue-Tek VIP 2000-3000 In-Reply-To: <56366.46162.qm@web114415.mail.gq1.yahoo.com> References: <56366.46162.qm@web114415.mail.gq1.yahoo.com> Message-ID: <782E3A02C2EB2347BEA6DEA69DC7AB866ACB5C5DAD@sfamail.SAKURAUS.LOCAL> Just to reinforce Joe's comments and clarify information regarding the VIP "K" series processors (VIP 1000, 2000 & 3000): - Sakura manufactured these for Miles until 1993. - The first two digits of the serial number identify the year it was manufactured.(82-92) - Never purchase a unit without a serial number affixed to the unit. - Sakura no longer services these units. - Parts are no longer being manufactured for these units. - However, many parts are still stocked by Sakura and available for purchase. - Some new or used parts may be available through independent service companies. - As Joe stated, be responsible with routine maintenance. - They've been around longer than the (trademarked) "Energizer Bunny." - The fact that many people still use these models is proof of their reliability. Kind Regards, Nancy Klemme (Another old work horse) Education / Training, Technical Services -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph Saby Sent: Friday, August 20, 2010 2:45 PM To: Fimbres, Amber; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue-Tek VIP 2000-3000 Mike- Although Sakura is no longer making parts for the VIP 2000 units, there are thousands still in use around the country. Most companies that service these units have been taking them in trade as people upgrade their equipment, then using these units to provide parts for units in trouble along the way. They are very dependable (with regular water rinses and periodic PM). Sakura does still makes parts for and service the VIP E300s, which is the step up from the 2000. If the price were right, and funds were low, I would buy either. I have been using these units for many years. Joe Saby, BA HT ________________________________ From: "Fimbres, Amber" To: "histonet@lists.utsouthwestern.edu" Sent: Fri, August 20, 2010 5:17:46 PM Subject: [Histonet] Tissue-Tek VIP 2000-3000 Mike, I know I'm a little late regarding your question about Sakura's VIP 2000/3000. I'm not sure if you found your answer, but if you're talking about the VIP processor that uses a magnet to program, change stations, etc. (and is reddish orange in color and may even say 'Miles' on it instead of Sakura) you will want to think twice before purchasing it. These VIP K (series 1000, 2000, 3000) are no longer serviced by Sakura nor are there many parts for them. Sakura's manufacturer for parts has stopped making replaceable parts for this particular model (they are at least 20 or more years old). You can go to Sakura's website or call their technical support, they will confirm this too. Take care, Amber ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Fri Aug 20 18:19:40 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Aug 20 18:20:01 2010 Subject: [Histonet] protocol for Masson-Goldner trichrome modified by Ulrich In-Reply-To: <616D0C3F5CEB486F8AC342F3277C716C@vandeberg> References: <616D0C3F5CEB486F8AC342F3277C716C@vandeberg> Message-ID: What are your histological endpoints? What is it you wish to demonstrate with this stain? Is it Rodent? Canine? Bone? Cartilage? Microtomed sections? Thick/Ground section? W/ or W/O implants? I can help you with the protocol, but I might be able to provide more assistance. Jack On Aug 20, 2010, at 2:15 PM, "Michael Mashore" wrote: > Hello everyone, > > > > I'm trying to use the Masson-Goldner Trichrome stain, and I came across a > paper that used one that was modified by Ulrich. There was no citation on > the paper, and I wanted more details to their protocol, i.e. how they made > their solutions. The paper is titled "Histology and research at the hard > tissue-implant interface using Technovit 9100 New embedding technique" by > Elmar Willbold > ser=4429&_coverDate=06%2F25%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_d > ocanchor=&view=c&_searchStrId=1436969926&_rerunOrigin=google&_acct=C00005960 > 2&_version=1&_urlVersion=0&_userid=4429&md5=ac8acdf1a211881097275a05690a4259 > #cor1> and Frank Witt. Does anyone know more details on this stain? We are > using it on hard tissue embedding, where we used methylmethacrylate. > > > > Thanks for the help! > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histotech <@t> imagesbyhopper.com Sun Aug 22 11:11:01 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sun Aug 22 11:11:12 2010 Subject: [Histonet] cryostat In-Reply-To: <4C6EA55F0200004D000CEE26@hsc-iagate1.health.unm.edu> Message-ID: Jeff, We have both the Leica CM1850 and the CM1850 UV. These are not top of the line models, but they work very well for us. You can find out more information on the unit at the following URL: http://www.leica-microsystems.com/products/histology-systems/sectioning/cryo stats/details/product/leica-cm1850-uv/ Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Thompson Sent: Friday, August 20, 2010 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat Hello, I am writing to ask about what the community views are on the best -or worst - cryostats. We may have the opportunity to purchase one for the first time in many years and we would like to get a good, solid, reliable model. We're not really looking for one (and can't really afford one) with all the bells and whistles, but would like to know what folks think about the ones to look for as well as the ones to steer clear of. All input is appreciated. Thanks, Jeff Thompson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3071 - Release Date: 08/15/10 18:35:00 From histotech <@t> imagesbyhopper.com Sun Aug 22 11:18:18 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sun Aug 22 11:18:29 2010 Subject: [Histonet] cryostat In-Reply-To: Message-ID: Jeff, please note that the URL posted below wrapped into two lines. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Sunday, August 22, 2010 12:11 PM To: 'Jeffrey Thompson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat Jeff, We have both the Leica CM1850 and the CM1850 UV. These are not top of the line models, but they work very well for us. You can find out more information on the unit at the following URL: http://www.leica-microsystems.com/products/histology-systems/sectioning/cryo stats/details/product/leica-cm1850-uv/ Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Thompson Sent: Friday, August 20, 2010 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat Hello, I am writing to ask about what the community views are on the best -or worst - cryostats. We may have the opportunity to purchase one for the first time in many years and we would like to get a good, solid, reliable model. We're not really looking for one (and can't really afford one) with all the bells and whistles, but would like to know what folks think about the ones to look for as well as the ones to steer clear of. All input is appreciated. Thanks, Jeff Thompson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3071 - Release Date: 08/15/10 18:35:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3087 - Release Date: 08/22/10 06:35:00 From histonetalias <@t> gmail.com Sun Aug 22 14:25:03 2010 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Sun Aug 22 14:25:10 2010 Subject: [Histonet] Histology in the Caribbean Message-ID: I am looking for any information on labs or hospitals that do histology in the Caribbean. Possibly the Turks and Caicos Islands, Bahamas, or USVI. Thanks! -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From WesterM <@t> MedImmune.com Mon Aug 23 08:12:46 2010 From: WesterM <@t> MedImmune.com (Wester, Martha) Date: Mon Aug 23 08:12:54 2010 Subject: [Histonet] RE: cryostat In-Reply-To: References: Message-ID: Jeff-- We have Leica CM3050's. They have lots of bells and whistles and we've found that most techs don't make full use of them anyway. But we've found the Leica product to be solid and have had no real troubles with them. Martha ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Message: 1 Date: Sun, 22 Aug 2010 12:11:01 -0400 From: Subject: RE: [Histonet] cryostat To: "'Jeffrey Thompson'" < >, Message-ID: Content-Type: text/plain; charset=us-ascii Jeff, We have both the Leica CM1850 and the CM1850 UV. These are not top of the line models, but they work very well for us. You can find out more information on the unit at the following URL: http://www.leica-microsystems.com/products/histology-systems/sectioning/ cryo stats/details/product/leica-cm1850-uv/ Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Thompson Sent: Friday, August 20, 2010 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat Hello, I am writing to ask about what the community views are on the best -or worst - cryostats. We may have the opportunity to purchase one for the first time in many years and we would like to get a good, solid, reliable model. We're not really looking for one (and can't really afford one) with all the bells and whistles, but would like to know what folks think about the ones to look for as well as the ones to steer clear of. All input is appreciated. Thanks, Jeff Thompson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3071 - Release Date: 08/15/10 18:35:00 ******************************* To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From MLashus <@t> pathgroup.com Mon Aug 23 08:29:21 2010 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Mon Aug 23 08:29:27 2010 Subject: [Histonet] Excellent Employment Opportunity Message-ID: <197CD0B02A81F94994A285C59C8AE05C05D6F4909E@pgnexchange.pathgroup.com> Outstanding opportunity for experienced Histotechnician/ Histotechnologist in our Nashville lab PathGroup and our affiliated physician group Associated Pathologists, offers a modern one-stop shop for the latest in sophisticated diagnostic laboratory services covering all aspects of clinical and anatomic pathology in the Southeast and lower Midwest. The mission of PathGroup is to provide the highest quality anatomic and clinical pathology services which consistently exceed the expectations of our clients, physicians, employees, payers, and most importantly, our patients. PathGroup is continuously seeking quality individuals who share our values. We currently have an opening for a full time Histotech on our night shift. We offer competitive salary and benefit package and generous shift differential. For consideration or for more information please contact kmustoe@pathgroup.com, 615-562-9315. ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From sfeher <@t> CMC-NH.ORG Mon Aug 23 08:43:26 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Aug 23 08:43:31 2010 Subject: [Histonet] Hawaii In-Reply-To: <20100820073658.9e2d9aa830e8449a2412eb1e4f2f067e.58c9ef4452.wbe@email04.secureserver.net> References: <20100820073658.9e2d9aa830e8449a2412eb1e4f2f067e.58c9ef4452.wbe@email04.secureserver.net> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F268@exchange.cmc-nh.org> Sara - You might want to check the Federal Government website for positions at the Army hospital there. They usually have plenty of openings for short supply medical specialist like histotechs. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Friday, August 20, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hawaii Does anyone know of any open positions in Hawaii, and what is the expected payscale there? I have a BA, HT, and a very good knowledge of IHC. I have 6 years registered experience with 2 years histo. lab =assistant before that. I know things are insane expensive. Me a nd my fiance might be moving there because of his job. Any help would=e awesome!! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 [DEL: (5=2)386-5107 :DEL] _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Mon Aug 23 08:44:39 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Aug 23 08:44:43 2010 Subject: [Histonet] Hawaii In-Reply-To: <20100820073658.9e2d9aa830e8449a2412eb1e4f2f067e.58c9ef4452.wbe@email04.secureserver.net> References: <20100820073658.9e2d9aa830e8449a2412eb1e4f2f067e.58c9ef4452.wbe@email04.secureserver.net> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F269@exchange.cmc-nh.org> Sorry Sara. Guess I should have read all of the posts regarding your question. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Friday, August 20, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hawaii Does anyone know of any open positions in Hawaii, and what is the expected payscale there? I have a BA, HT, and a very good knowledge of IHC. I have 6 years registered experience with 2 years histo. lab =assistant before that. I know things are insane expensive. Me a nd my fiance might be moving there because of his job. Any help would=e awesome!! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 [DEL: (5=2)386-5107 :DEL] _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKnutson <@t> primecare.org Mon Aug 23 09:03:53 2010 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Mon Aug 23 09:04:03 2010 Subject: [Histonet] Productivity Measurement Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2A647C69A@EXCHANGE2K7.staprimecare.org> Hello Fellow Histonetters, I am curious if any of you have a measurement level that your institution uses to show PA's their productivity level? I realize that much of it depends on the type of specimen and what is found in the specimen. Biopsies can be grossed in at greater speed then the more complex cases. Is there an established average productivity that a PA should be able to attain? So many cases per hour? Would be curious if any of you have some input to share on this subject. Thanks for responding. Deanne Knutson Anatomic Pathology Supervisor (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From Pat.Patterson <@t> propath.com Mon Aug 23 09:13:44 2010 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Mon Aug 23 09:13:49 2010 Subject: [Histonet] Dallas IHC Position Message-ID: <82C7248978CB50469FD6BA68EBBEFE6703F73206@exchange.propathlab.com> IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. We require a minimum of 4 years Histology experience with at least 1-2 years immunohistochemistry, immunofluorescence or in situ hybridization experience. Working knowledge of IHC theory required and hands on IHC performance desired, if using an automated system we'll easily train you on our manual system, Leadership abilities preferred. HT (ASCP) or QIHC desired. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Our benefits include medical, dental, company paid short-term and long-term disability insurance, a matched 401K plan and more! Mon-Fri 7pm-3:30am For consideration send resume to: ProPath, Human Resources 1355 River Bend Drive Dallas, TX 75247 Fax - 214/237-1825 Job Line - 214/237-1775 jobs@propath.com Thanks! Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com From rjbuesa <@t> yahoo.com Mon Aug 23 09:23:12 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 23 09:23:17 2010 Subject: [Histonet] Productivity Measurement In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F76833A2A647C69A@EXCHANGE2K7.staprimecare.org> Message-ID: <956076.38367.qm@web65704.mail.ac4.yahoo.com> As you wrote it depends on the type of specimen, and institution as well. Under separate cover I am sending something about staffing in histology. Ren? J. --- On Mon, 8/23/10, Knutson, Deanne wrote: From: Knutson, Deanne Subject: [Histonet] Productivity Measurement To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, August 23, 2010, 10:03 AM Hello Fellow Histonetters, I am curious if any of you have a measurement level that your institution uses to show PA's their productivity level?? I realize that much of it depends on the type of specimen and what is found in the specimen.? Biopsies can be grossed in at greater speed then the more complex cases.? Is there an established average productivity that a PA should be able to attain?? So many cases per hour?? Would be curious if any of you have some input to share on this subject.? Thanks for responding. Deanne Knutson Anatomic Pathology Supervisor (701)-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Mon Aug 23 09:36:56 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Aug 23 09:37:00 2010 Subject: [Histonet] Frozen Section Images Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F270@exchange.cmc-nh.org> Are any of you using equipment that will send an image of a frozen section diagnosis directly to the LCD monitors located in the Operating Room? We will be attempting to have the capability to do this by using a Leica digital camera that is interfaced to a CPU and attached to a microscope in a room adjacent to our OR suites. The idea is for the surgeon to be able to see the cellular detail of the frozen section diagnosis. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From CIngles <@t> uwhealth.org Mon Aug 23 09:51:07 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Mon Aug 23 09:54:29 2010 Subject: [Histonet] Corrective Action Form References: <57BE698966D5C54EAE8612E8941D76830957B29D@EXCHANGE3.huntingtonhospital.com> Message-ID: Laurie: We just have a little form made out that we keep by the QA sheets for the stainer that lists date, problem encountered, and what the resolution was. That is for H&E's though. Immunos may be a bit more complicated. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Colbert Sent: Thu 8/19/2010 4:26 PM To: Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Corrective Action Form I'm looking at it from the standpoint of an inspector - we had a problem and what steps did we follow to correct it and prevent it from happening again. I'm not planning on using it as a punishment for anyone. BTW, can anyone expalain to me how false positives come about on immunos? Claire From Rhonda.Hartz <@t> saskatoonhealthregion.ca Mon Aug 23 10:42:13 2010 From: Rhonda.Hartz <@t> saskatoonhealthregion.ca (Hartz, Rhonda SktnHR) Date: Mon Aug 23 10:42:46 2010 Subject: [Histonet] Please unsubscribe Message-ID: Please unsubscribe. Rhonda Hartz Technologist Supervisor Anatomic Pathology Saskatoon Health Region From integrated.histo <@t> gmail.com Mon Aug 23 11:43:45 2010 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Mon Aug 23 11:43:53 2010 Subject: [Histonet] CBG Formalin Recycling Reagents Message-ID: As we are cleaning out our lab, I have come across some CBG formalin recycling reagents / filters. If you use a CBG formalin recycler, I will be happy to send the items to you. You will need to contact Fed Ex and arrange a pick-up by this Thursday. This can be done online and the label e-mailed to me. Cindy DuBois Integrated Pathology 2291 W MARCH LN STE E-101 STOCKTON, CA 95207 From wdesalvo.cac <@t> hotmail.com Mon Aug 23 12:25:24 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Mon Aug 23 12:25:30 2010 Subject: [Histonet] Productivity Measurement In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F76833A2A647C69A@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F76833A2A647C69A@EXCHANGE2K7.staprimecare.org> Message-ID: The productivity measurement for your PA's will be different from other sites as you must consider and account for the critical factors that will dictate their productivity. This process, as all others in Histology, does not have one set protocol/procedure accepted by all and you cannot successfully evaluate staff on a "standard" that may be developed using different factors. You must measure what is happening in your lab and then set your "standard" and quality expectations. You have to take into account all "critical to success factors" for the task/bench (i.e. competency of staff, type/mix of specimens, the grossing protocol set by the Pathologist, number of specimens required for a tissue type, amount of non-gross dissection work performed on each specimen, computer interaction time) and then, I suggest, monitor on a daily basis to develop the "standard" for your lab. There will be a difference in the workload and work flow each day. I also suggest once you collect enough data to trend the task productivity, develop a "minimum expectation" standard that is a combination or productivity and quality and can be performed consistently for 2 hours at a time. There should be a break from the task after 2 hours or you will see a decrease in productivity and an increase in the possibility to create an error. The "minimum standard" will be a rate that all competent and trained PA's can achieve an maintain consistently. You then manage to the minimum standard and this standard should be evaluated on a regular basis and adjusted if and when the critical to success factors change. If you take the time to develop a process for recording, evaluating and managing workload and work flow in you lab, you will be rewarded with increased employee satisfaction, management satisfaction and most importantly client/patient satisfaction, not to mention the increase in quality. William DeSalvo, B.S., HTL(ASCP) Chair, NSH Quality Control Committee > From: DKnutson@primecare.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 23 Aug 2010 09:03:53 -0500 > Subject: [Histonet] Productivity Measurement > > Hello Fellow Histonetters, > > I am curious if any of you have a measurement level that your institution uses to show PA's their productivity level? I realize that much of it depends on the type of specimen and what is found in the specimen. Biopsies can be grossed in at greater speed then the more complex cases. Is there an established average productivity that a PA should be able to attain? So many cases per hour? Would be curious if any of you have some input to share on this subject. Thanks for responding. > > Deanne Knutson > Anatomic Pathology Supervisor > (701)-530-6730 > dknutson@primecare.org > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.thompson <@t> student.ucc.ie Mon Aug 23 13:12:31 2010 From: c.thompson <@t> student.ucc.ie (c.thompson@student.ucc.ie) Date: Mon Aug 23 13:12:40 2010 Subject: [Histonet] Please unsubscribe Message-ID: <4c72ba0f.398.336f.674209773@student.ucc.ie> From MadaryJ <@t> MedImmune.com Mon Aug 23 13:31:41 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Aug 23 13:31:47 2010 Subject: [Histonet] is there a resource to share info about bad equipment vendor experiences? Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1302400B9F@MD1EV002.medimmune.com> I do not want to generate anything negative, but I would like to share positive and negative experiences regarding vendors or equipment to help and/or warn other techs. I have shared positive info on vendors here on the net, but I recall there being a problem with someone submitting a negative comment a few years back. How can we help others if we have a piece of equipment that should never have been marketed, or a company that does not honor a warranty etc? I know it happens, just would like to know a legal and ethical way of sharing this with fellow histologists. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From ryeo <@t> wchosp.org Mon Aug 23 13:43:30 2010 From: ryeo <@t> wchosp.org (Richard Yeo) Date: Mon Aug 23 13:45:40 2010 Subject: [Histonet] (no subject) Message-ID: Hey Histo Gurus Can any one tell me what the salary scale in the mid west to the north east region is for a Mohs Tech? We are thinking about starting a mohs lab for a local Dematologist. Thanks Rich Y From patrick.lewis <@t> seattlechildrens.org Mon Aug 23 14:03:37 2010 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Mon Aug 23 14:04:16 2010 Subject: [Histonet] Greetings and IMHC Questions Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C0571C657@s107.childrens.sea.kids> Hello everyone, My name is Patrick Lewis and I just started working for Children's Hospital in their Research Department. It's been a long time since I've done any Immunohistochemistry, (~10 years), but in this new job, I will be doing a lot of it with both paraffin and frozen sections that I will make myself. Anyway, I am about to try staining my first frozen slides and I have a couple of quick questions. I cut some practice slides, human tonsil, and have stored them at -80 in a slide box. How much longevity will they have? I'd like to be able to cut slides and store them unfixed at -80 for up to one month until I use them I know its better to use them the same day, but it would be nice to be able to cut them on one day and use them as needed Also, is there a good place to find general IMHC procedures? It's been a long time and I want to re-familiarize myself with some general protocols I am using Superfrost Plus slides, but we may have some problems with the sections falling off the slide. Does anyone have any suggestions on methods to avoid tissue loss from the slide? Also, I am freezing my tissues in OCT in cryomolds that are lowered into an iso-pentane filled ? coke can which is immersed in a bucket of dry-ice for 5 minutes. . I wanted to avoid using liquid nitrogen and am hoping that with the dry ice method I would still get good freezing of the block. Are there any recommendations for OCT freezing? Thanks for all your help. Patrick. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rags_jeg <@t> yahoo.co.in Mon Aug 23 14:30:08 2010 From: rags_jeg <@t> yahoo.co.in (vaigunda ragavendran) Date: Mon Aug 23 14:30:15 2010 Subject: [Histonet] Re: Research Assistant/Technician Position Message-ID: <183556.47999.qm@web8706.mail.in.yahoo.com> Hello everyone, I am on the lookout for a suitable research tech/asst position for my wife who holds a master's in microbial gene technology... I am a postdoc at the U of S in saskatoon and am trying to find a position for her at saskatoon.. Please let me know if you happen to come across any openings.. Buh byee.. Rags, Postdoctoral Research Fellow, Verge Lab, Department of Anatomy and Cell Biology, Room No: 5800, Saskatoon City Hospital, 701, Queen Street, Saskatoon, Saskatchewan S7K 0M7, Canada. Phone (Lab): +1 306-655-8714 ????????? (Facsimile): +1 306-655-8709 From sgoebel <@t> xbiotech.com Mon Aug 23 14:30:53 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Aug 23 14:30:57 2010 Subject: [Histonet] (no subject) Message-ID: <20100823123053.9e2d9aa830e8449a2412eb1e4f2f067e.96d41cda50.wbe@email04.secureserver.net> Down here in Texas it starts around $25 an hour and goes up with years of experience. I've seen some for up to $40 an hour and I think Sarah Goebel, B.A., HT (ASCP) XBiotech USA Inc. 8201 East < (512)386-5107 -------- Original Message -------- Subject: [Histonet] (no subject) From: "Richard Yeo" <[1]ryeo@wchosp.org< Date: Mon, August 23, 2010 11:43 am To: <[2]Histonet@lists Hey Histo Gurus Can any one tell me what the salary scale in the mid west to the north east We are thinking about starting a mohs lab for a local Dematologist. Thanks Rich Y _______________________________________________ Histonet mailing list [3]Histonet@lists.utsouth [4]http: References 1. 3D"mailto:ryeo@wchosp.org" 2. 3D"mailto:Histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From talulahgosh <@t> gmail.com Mon Aug 23 14:35:28 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Aug 23 14:35:33 2010 Subject: [Histonet] RE: cryostat In-Reply-To: References: Message-ID: I concur, definitely Leica. The 3050's are the more expensive ones, but I found the way they are lower to the ground much easier to section in. The 1850 is much higher up. But it does cost less. Also, we recently had to buy the more expensive 3050 (the one with a sectioning motor) because they do not make the 3050 without a motor anymore. We don't use the motor but really like the 3050 style, and had some leftover money, so we paid more to not use the motor. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx From rmweber113 <@t> comcast.net Mon Aug 23 15:07:03 2010 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Mon Aug 23 15:07:09 2010 Subject: [Histonet] job opportunity In-Reply-To: <543110356.374473.1282593830929.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Message-ID: <1481833672.374715.1282594023164.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> I have a job opportunity for a new urology in office pathology laboratory in North Jersey.? Candidate must be ASCP certified.? Candidate will be responsible for all? phases of histology to include cytology and FISH testing.? Flexible hours M-F with full benefit package.? Please fax resume to number below or call. Thank you, Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 From kimtournear <@t> yahoo.com Mon Aug 23 15:27:03 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Mon Aug 23 15:27:07 2010 Subject: [Histonet] FOXO3a Message-ID: <626040.4761.qm@web54205.mail.re2.yahoo.com> Hi, Is/has anyone tried running the FOXO3a (from Cell Signaling)?maunally or automated? I'm trying to work this up on the BondMax. Any protocols would be appreciated, manually and/or auotmated. Thanks in advance!! ~Kim~ ~Don't be afraid your life will?end, be afraid it will never begin~ OU Rocks!!!! From Albert.Santiago <@t> uphs.upenn.edu Mon Aug 23 18:12:23 2010 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Mon Aug 23 18:12:27 2010 Subject: [Histonet] In-Situ Hybridization Regulations Message-ID: Hello fellow histonetters, our lab is accredited by The TJC and I'd like to know if anyone knows whether automated in-situ hybridization staining requires any type of special joint commission regulation or does it just fall under the moderate or high complexity testing such as Immunohistochemistry? If you do, could you please refer me to some literature on the subject. Thank you The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From k84as <@t> yahoo.com Mon Aug 23 21:49:27 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Mon Aug 23 21:49:31 2010 Subject: [Histonet] how to be qualified? Message-ID: <251771.42362.qm@web112619.mail.gq1.yahoo.com> Dear histonetters As i'm in Egypt and going to end my master degree in Vet.?histology to be ass. lecturer in Cairo Univ. i'm looking for an opportunity to be ass. researcher or associat?in histotechnology feild abroad in USA or Canada can?i wish you?help me to find it. so am i have to be ASCP cert. to work as i have master degree?? if yes i have searched there site but can't find where i can pass the exams in Egypt!! can you send me a link if you reached it ortell me?how to be certified if i'm in Egypt?? ? thanx in advance Mohamed Abd El Razik Histology Dep. Faculty of Vet. Med. Cairo Univ.- Egypt 020105061378 From nasir_farooq2002 <@t> yahoo.com Tue Aug 24 03:18:51 2010 From: nasir_farooq2002 <@t> yahoo.com (nasir farooq) Date: Tue Aug 24 03:18:54 2010 Subject: [Histonet] (no subject) Message-ID: <468160.55982.qm@web59608.mail.ac4.yahoo.com> plz send me the latest updates From kimtournear <@t> yahoo.com Tue Aug 24 06:39:57 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Aug 24 06:40:06 2010 Subject: [Histonet] how to be qualified? In-Reply-To: <251771.42362.qm@web112619.mail.gq1.yahoo.com> Message-ID: <216441.29982.qm@web54204.mail.re2.yahoo.com> Hi Mohmed, I think the ASCP is only offered in the US. I don't think a certification is required in order to teach at a university here, but then again, it might depend on the University. If you go to ASCP's web site, there should be a place for you to request an application for the exam. The problem is, you would have to be here in the US to take the exam.?I don't think they offer it on line. ? I found the following 2?links when I googled "histology board certification in Egypt": ? http://www.zu.edu.eg/Plastination/index.htm http://journals.indexcopernicus.com/karta.php?action=masterlist&id=1236 ? You might want to check with the Egyptian Society of Histotechnology or The Egyptian Journal of Histology about certification assistance. ? Hope this helps. Good luck!! ~Kim~ ~Don't be afraid your life will?end, be afraid it will never begin~ OU Rocks!!!! --- On Mon, 8/23/10, mohamed abd el razik wrote: From: mohamed abd el razik Subject: [Histonet] how to be qualified? To: Histonet@lists.utsouthwestern.edu Date: Monday, August 23, 2010, 7:49 PM Dear histonetters As i'm in Egypt and going to end my master degree in Vet.?histology to be ass. lecturer in Cairo Univ. i'm looking for an opportunity to be ass. researcher or associat?in histotechnology feild abroad in USA or Canada can?i wish you?help me to find it. so am i have to be ASCP cert. to work as i have master degree?? if yes i have searched there site but can't find where i can pass the exams in Egypt!! can you send me a link if you reached it ortell me?how to be certified if i'm in Egypt?? ? thanx in advance Mohamed Abd El Razik Histology Dep. Faculty of Vet. Med. Cairo Univ.- Egypt 020105061378 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Tue Aug 24 09:27:13 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Aug 24 09:26:34 2010 Subject: [Histonet] Sr Histotech Job in Miami Message-ID: <1831224095.1282660033546.JavaMail.cfservice@sl4app3> 1st shift, Sr Histotechnologist position in Miami, Florida. This position will be responsible for coordinating and supervising the Immunohistochemistry of the lab section. For consideration you must have a minimum of 5 years of IHC experience and a Florida state Histology license. Lead or supervisory experience and QIHC certification are preferred but not required. This position is with a 700 bed, award winning hospital that is part of South Florida's leading Health System. My client is offering a competitive compensation package and excellent benefits. Please contact me if you're be interested in hearing more about this position and learning if it might be the next step in your career. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From ree3 <@t> leicester.ac.uk Tue Aug 24 10:17:54 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Tue Aug 24 10:17:58 2010 Subject: [Histonet] shrinkage Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8D777B9CA@EXC-MBX3.cfs.le.ac.uk> Anybody aware of the degree of shrinkage in paraffin processed tissues and/or GMA processed tissues?, many thanks. Cheers Richard Edwards Leicester University. Leicester U.K. From Bill.Tench <@t> pph.org Tue Aug 24 10:32:58 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Tue Aug 24 10:33:09 2010 Subject: [Histonet] shrinkage Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5541@MAIL1.pph.local> I have never seen a study, but when training, we were taught that fixation and processing could result in up to 5-10% shrinkage. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From mcauliff <@t> umdnj.edu Tue Aug 24 10:38:14 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Aug 24 10:35:34 2010 Subject: [Histonet] shrinkage In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1E8D777B9CA@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A1E8D777B9CA@EXC-MBX3.cfs.le.ac.uk> Message-ID: <4C73E766.50404@umdnj.edu> Shrinkage for paraffin processed tissues is about 20%. For GMA it is less than 5% which is one reason GMA stuff looks so nice. Geoff Edwards, Richard E. wrote: > Anybody aware of the degree of shrinkage in paraffin processed tissues and/or GMA processed tissues?, many thanks. > > Cheers > Richard Edwards > > Leicester University. > > Leicester U.K. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From gu.lang <@t> gmx.at Tue Aug 24 10:49:54 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Aug 24 10:50:01 2010 Subject: AW: [Histonet] shrinkage In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A5541@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A5541@MAIL1.pph.local> Message-ID: <31CBD58578D148EDAA643BE7027CE94A@dielangs.at> I think tissue shrinkage is a fairy tail, told by pathologists at the grossing. ;) "Sure, it is a little bit large, but it will shrink.." Too big blocks stay too big while embedding. **just for smiling** There's a study by Cecil Fox 1985 (Formaldehyde fixation), who showed that shrinkage while fixation occurs only with concentrated formaldehyd, perhaps due to the methanol-part in commercial formalin. For dehydration, "de-fattation" and infiltration I think, it depends on the gradationsteps of the solutions, the applied temperature and the "ingredients" of the tissue. With our standard protocol (13 hours VIP) I never found excessiv shrinkage. So, like always, it depends... Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Tench, Bill Gesendet: Dienstag, 24. August 2010 17:33 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] shrinkage I have never seen a study, but when training, we were taught that fixation and processing could result in up to 5-10% shrinkage. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Aug 24 10:51:07 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Aug 24 10:51:09 2010 Subject: [Histonet] RELIA Histology Job Alert 8/24/2010 Message-ID: Hi Histonetters!, I hope you are having a great day. I wanted to tell you about a new position that I am excited about. I am currently working with a private dermatopathology lab in Cincinnati, OH They are in need of a Mohs Histotech. This is a full time M-F daytime position If you or anyone you know might be interested in hearing more about this opportunity please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From MSHERWOOD <@t> PARTNERS.ORG Tue Aug 24 11:06:31 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Aug 24 11:05:50 2010 Subject: [Histonet] shrinkage In-Reply-To: <4C73E766.50404@umdnj.edu> References: <7722595275A4DD4FA225B92CDBF174A1E8D777B9CA@EXC-MBX3.cfs.le.ac.uk> <4C73E766.50404@umdnj.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E245BA@PHSXMB30.partners.org> We found shrinkage noticeable in our cornea samples. We get some corneas that have had measurements done before fixing and then, when comparing the corneas after processing, the measurements are slightly different. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Tuesday, August 24, 2010 11:38 AM To: Edwards, Richard E. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] shrinkage Shrinkage for paraffin processed tissues is about 20%. For GMA it is less than 5% which is one reason GMA stuff looks so nice. Geoff Edwards, Richard E. wrote: > Anybody aware of the degree of shrinkage in paraffin processed tissues and/or GMA processed tissues?, many thanks. > > Cheers > Richard Edwards > > Leicester University. > > Leicester U.K. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Maria.Mejia <@t> ucsf.edu Tue Aug 24 12:31:33 2010 From: Maria.Mejia <@t> ucsf.edu (Mejia, Maria) Date: Tue Aug 24 12:34:07 2010 Subject: [Histonet] Re: Cryoprotection In-Reply-To: <160206.43907.qm@web8701.mail.in.yahoo.com> References: <160206.43907.qm@web8701.mail.in.yahoo.com> Message-ID: Can you be more specific & tell us what DW means & please give us the protocol for the sucrose including the amount of DW used. This way we can help you. Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF San Francisco, CA ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of vaigunda ragavendran [rags_jeg@yahoo.co.in] Sent: Friday, August 20, 2010 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Cryoprotection Hii, If instead of sucrose in PBS, we use sucrose in DW by mistake, what might happen to fixed tissue sections during cryoprotection.. Please lemme know.. Rags, Postdoctoral Research Fellow, Verge Lab, Department of Anatomy and Cell Biology, Room No: 5800, Saskatoon City Hospital, 701, Queen Street, Saskatoon, Saskatchewan S7K 0M7, Canada. Phone (Lab): +1 306-655-8714 (Facsimile): +1 306-655-8709 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmjohnson34 <@t> hotmail.com Tue Aug 24 12:52:21 2010 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Tue Aug 24 12:52:26 2010 Subject: [Histonet] Alcian Yellow Message-ID: Can I get someone to share their source for Alcian Yellow? Our Pathologist wants to try a different method for Helicobacter and I need powdered AY? Thanks, Jennifer From jaylundgren <@t> gmail.com Tue Aug 24 13:09:28 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Aug 24 13:09:34 2010 Subject: [Histonet] shrinkage In-Reply-To: <31CBD58578D148EDAA643BE7027CE94A@dielangs.at> References: <2820431BF953BB4DA3E9E1A5882265FD034A5541@MAIL1.pph.local> <31CBD58578D148EDAA643BE7027CE94A@dielangs.at> Message-ID: I was also taught 10% shrinkage in histo school. Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue Aug 24 13:30:31 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Aug 24 13:30:36 2010 Subject: [Histonet] Ventana vs Leica Message-ID: I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) From bhewlett <@t> cogeco.ca Tue Aug 24 13:58:12 2010 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Aug 24 13:58:24 2010 Subject: [Histonet] shrinkage References: <7722595275A4DD4FA225B92CDBF174A1E8D777B9CA@EXC-MBX3.cfs.le.ac.uk> Message-ID: <05BC86D39A33480EB25696CD70FFD979@mainbox> Richard, It depends upon the tissue type, the fixative used, time/temperature/ gradation of alcohols, intermediate solvent type/time/temp and the wax time/temp. Careful fixation and processing of liver or spleen, using 4% aqueous formaldehyde/ graded alcohols /xylene/ wax at 56-60C, results in mean volume changes of 30-40%. This is discussed in JR Bakers seminal text "Principles of Biological Microtechnique" 1958. try to find a copy in your library. You will also find discussion in the EM literature from the 1960's. I recall 3-8% for resins. Regards, Bryan ----- Original Message ----- From: "Edwards, Richard E." To: Sent: Tuesday, August 24, 2010 11:17 AM Subject: [Histonet] shrinkage Anybody aware of the degree of shrinkage in paraffin processed tissues and/or GMA processed tissues?, many thanks. Cheers Richard Edwards Leicester University. Leicester U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> shorememorial.org Tue Aug 24 14:04:30 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Tue Aug 24 14:07:12 2010 Subject: [Histonet] Ventana vs Leica In-Reply-To: Message-ID: Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren To Sent by: histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Tue Aug 24 14:23:23 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Aug 24 14:23:30 2010 Subject: [Histonet] Re: Cryoprotection Message-ID: <20100824122323.9e2d9aa830e8449a2412eb1e4f2f067e.9f75b4c822.wbe@email04.secureserver.net> I assumed distilled water? Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 E Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] Re: Cryoprotection From: "Mejia, Maria" <[1]Maria.Meji Date: Tue, August 24, 2010 10:31 am To: vaigunda ragavendran <[2]rags_j "[3]histonet@lists.utsout histonet@lists.utsouthwestern.edu> Can you be more specific & tell us what DW means & please give us t he protocol for the sucrose including the amount of DW used. This way we can help you. Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF San Francisco, CA ________________________________________ From: [5]histonet [[6]histonet-bounces@lists.utsouthwestern.edu] On ragavendran [[7]rag Sent: Friday, August 20, 2010 1:19 PM To: [8]histonet@lists.uts Subject: [Histonet] Re: Cryoprotection Hii, If instead of sucrose in PBS, we use sucrose in DW by mistake, what might h Please lemme know.. Rags, Postdoctoral Research Fellow, Verge Lab, Department of Anatomy and Cell Biology, Room No: 5800, Saskatoon City Hospital, 701, Queen Street, Saskatoon, Saskatchewan S7K 0M7, Canada. Phone (Lab): +1 306-655-8714 (Facsimile): +1 306-655-8709 _______________________________________________ Histonet mailing list [9]Histonet@lists.utsouth [10]http: _______________________________________________ Histonet mailing list [11]Histonet@lists.utsouth [12]http: References 1. 3D"mailto:Maria.Mejia@ucsf.edu" 2. 3D"mailto:rags_jeg@yahoo.co.in" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:histonet-bounces@l 7. 3D"mailto:rags_jeg@yahoo.co.in" 8. 3D"mailto:histonet@lists.utsouthwestern.edu" 9. 3D"mailto:Histonet@lists.utsouthwestern.edu" 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 11. 3D"mailto:Histonet@lists.utsouthwestern.edu" 12. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Maria.Katleba <@t> stjoe.org Tue Aug 24 14:32:08 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Aug 24 14:35:17 2010 Subject: [Histonet] Ventana vs Leica In-Reply-To: References: Message-ID: Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. Does IHC and ISH (yes- so does the Ventana) 2. Continuous feed (Ventana does NOT offer this!!!!) 3. Space saver, much smaller footprint than Benchmark 4. No wasted antibodies... 5. Not forced to buy EXPENSIVE prep kits either.... 6. Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. Won't blow tissue off the slide! 8. No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren To Sent by: histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From rags_jeg <@t> yahoo.co.in Tue Aug 24 14:35:41 2010 From: rags_jeg <@t> yahoo.co.in (vaigunda ragavendran) Date: Tue Aug 24 14:35:50 2010 Subject: [Histonet] Re: Cryoprotection In-Reply-To: <20100824122323.9e2d9aa830e8449a2412eb1e4f2f067e.9f75b4c822.wbe@email04.secureserver.net> Message-ID: <957619.90431.qm@web8703.mail.in.yahoo.com> Yes, I meant distilled water.. but now there is no problem.. I just checked my cut sections that were mistakenly cryoprotected in sucrose/DW instead of in sucrose/PBS and there is not a significant degree of cell lysis.. the dorsal root ganglion neurons are mostly intact and not lysed.. I felt happy yest and decided to be more careful the next time I do cryoprotection of my tissues.. Anyway thanks Sarah for your comment.. Byee.. Rags, Postdoctoral Research Fellow, Verge Lab, Department of Anatomy and Cell Biology, Room No: 5800, Saskatoon City Hospital, 701, Queen Street, Saskatoon, Saskatchewan S7K 0M7, Canada. Phone (Lab): +1 306-655-8714 ????????? (Facsimile): +1 306-655-8709 --- On Wed, 25/8/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: RE: [Histonet] Re: Cryoprotection To: "Mejia,Maria" Cc: "vaigunda ragavendran" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, 25 August, 2010, 12:53 AM I assumed distilled water? Sarah Goebel, B.A., HT (ASCP)Histotechnician XBiotech USA Inc.8201 East Riverside Dr. Bldg 4 Suite 100Austin, Texas? 78744(512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] Re: Cryoprotection From: "Mejia, Maria" Date: Tue, August 24, 2010 10:31 am To: vaigunda ragavendran , "histonet@lists.utsouthwestern.edu" Can you be more specific & tell us what DW means & please give us the protocol for the sucrose including the amount of DW used. This way we can help you. Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF San Francisco, CA ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of vaigunda ragavendran [rags_jeg@yahoo.co.in] Sent: Friday, August 20, 2010 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Cryoprotection Hii, If instead of sucrose in PBS, we use sucrose in DW by mistake, what might happen to fixed tissue sections during cryoprotection.. Please lemme know.. Rags, Postdoctoral Research Fellow, Verge Lab, Department of Anatomy and Cell Biology, Room No: 5800, Saskatoon City Hospital, 701, Queen Street, Saskatoon, Saskatchewan S7K 0M7, Canada. Phone (Lab): +1 306-655-8714 (Facsimile): +1 306-655-8709 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Tue Aug 24 14:42:04 2010 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Aug 24 14:44:42 2010 Subject: [Histonet] shrinkage In-Reply-To: <31CBD58578D148EDAA643BE7027CE94A@dielangs.at> References: <2820431BF953BB4DA3E9E1A5882265FD034A5541@MAIL1.pph.local> <31CBD58578D148EDAA643BE7027CE94A@dielangs.at> Message-ID: When I wrote my thesis on sweat glands, my experience was that fixation in Helly's fluid ("Zenker-formol") and paraffin embedding caused serious shrinkage: about 10-15% in each dimension. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, August 24, 2010 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] shrinkage I think tissue shrinkage is a fairy tail, told by pathologists at the grossing. ;) "Sure, it is a little bit large, but it will shrink.." Too big blocks stay too big while embedding. **just for smiling** There's a study by Cecil Fox 1985 (Formaldehyde fixation), who showed that shrinkage while fixation occurs only with concentrated formaldehyd, perhaps due to the methanol-part in commercial formalin. For dehydration, "de-fattation" and infiltration I think, it depends on the gradationsteps of the solutions, the applied temperature and the "ingredients" of the tissue. With our standard protocol (13 hours VIP) I never found excessiv shrinkage. So, like always, it depends... Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Tench, Bill Gesendet: Dienstag, 24. August 2010 17:33 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] shrinkage I have never seen a study, but when training, we were taught that fixation and processing could result in up to 5-10% shrinkage. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Tue Aug 24 14:47:57 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Tue Aug 24 14:48:02 2010 Subject: [Histonet] Ventana vs Leica In-Reply-To: Message-ID: <576316855.263995.1282679277534.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I totally agree and we have an Ultra, 2 XTs and 3 Benchmarks.? They are good systems that need to get cheaper and more flexible.? I am demoing the Bond III and love the ease of use, flexibility and cost savings, most.? Maria is right it is about 40% less to run than a Ventana. Pam Marcum AP Manager UAMS Little Rock, AR ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. ? ? ? ?Does IHC and ISH (yes- so does the Ventana) 2. ? ? ? Continuous feed (Ventana does NOT offer this!!!!) 3. ? ? ? Space saver, much smaller footprint than Benchmark 4. ? ? ? No wasted antibodies... 5. ? ? ? Not forced to buy EXPENSIVE prep kits either.... 6. ? ? ? Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. ? ? ? Won't blow tissue off the slide! 8. ? ? ? No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, ?I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 ?? ? ? ? ? ? Jay Lundgren ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? To ?? ? ? ? ? ? Sent by: ? ? ? ? ? ? ? ? ?histonet ?? ? ? ? ? ? histonet-bounces@ ? ? ? ? ?? ? ? ? ? ? lists.utsouthwest ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?cc ?? ? ? ? ? ? ern.edu ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Subject ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? [Histonet] Ventana vs Leica ?? ? ? ? ? ? 08/24/2010 02:30 ?? ? ? ? ? ? PM ?? ? I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. ?I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. ?If this is you, could you please tell me which you preferred and why. ?? ? I'm currently working for a facility in MT which has narrowed down its search to these two instruments. ?No vendors please, they've already given their pitches. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Thanks, ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. ? ? ? ?Does IHC and ISH (yes- so does the Ventana) 2. ? ? ? Continuous feed (Ventana does NOT offer this!!!!) 3. ? ? ? Space saver, much smaller footprint than Benchmark 4. ? ? ? No wasted antibodies... 5. ? ? ? Not forced to buy EXPENSIVE prep kits either.... 6. ? ? ? Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. ? ? ? Won't blow tissue off the slide! 8. ? ? ? No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, ?I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 ?? ? ? ? ? ? Jay Lundgren ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? To ?? ? ? ? ? ? Sent by: ? ? ? ? ? ? ? ? ?histonet ?? ? ? ? ? ? histonet-bounces@ ? ? ? ? ?? ? ? ? ? ? lists.utsouthwest ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?cc ?? ? ? ? ? ? ern.edu ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Subject ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? [Histonet] Ventana vs Leica ?? ? ? ? ? ? 08/24/2010 02:30 ?? ? ? ? ? ? PM ?? ? I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. ?I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. ?If this is you, could you please tell me which you preferred and why. ?? ? I'm currently working for a facility in MT which has narrowed down its search to these two instruments. ?No vendors please, they've already given their pitches. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Thanks, ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue Aug 24 15:21:59 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Aug 24 15:22:10 2010 Subject: [Histonet] RE: Shrinkage Message-ID: <000901cb43ca$0344b3c0$09ce1b40$@callis@bresnan.net> I have a publication on shrinkage of tissue, in particular decalcified bone, after paraffin processing with a figure of 25%. However I must agree with Bryan Hewlett on all the parameters he discussed. Gayle M. Callis HTL /HT/MT(ASCP) Bozeman MT From trathborne <@t> somerset-healthcare.com Tue Aug 24 16:10:27 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Aug 24 16:10:36 2010 Subject: [Histonet] Ventana vs Leica In-Reply-To: <576316855.263995.1282679277534.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: We recently acquired the Bond III. It was a tough decision, but space was the main reason we went with Leica. The Ventana sales team that visited our lab was very eager to get the account. Their pricing was not much different. They even offered to credit us for additional waste disposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pamela Marcum Sent: Tuesday, August 24, 2010 3:48 PM To: Maria Katleba Cc: histonet; BSullivan@shorememorial.org; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica I totally agree and we have an Ultra, 2 XTs and 3 Benchmarks.? They are good systems that need to get cheaper and more flexible.? I am demoing the Bond III and love the ease of use, flexibility and cost savings, most.? Maria is right it is about 40% less to run than a Ventana. Pam Marcum AP Manager UAMS Little Rock, AR ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. ? ? ? ?Does IHC and ISH (yes- so does the Ventana) 2. ? ? ? Continuous feed (Ventana does NOT offer this!!!!) 3. ? ? ? Space saver, much smaller footprint than Benchmark 4. ? ? ? No wasted antibodies... 5. ? ? ? Not forced to buy EXPENSIVE prep kits either.... 6. ? ? ? Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. ? ? ? Won't blow tissue off the slide! 8. ? ? ? No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, ?I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 ?? ? ? ? ? ? Jay Lundgren ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? To ?? ? ? ? ? ? Sent by: ? ? ? ? ? ? ? ? ?histonet ?? ? ? ? ? ? histonet-bounces@ ? ? ? ? ?? ? ? ? ? ? lists.utsouthwest ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?cc ?? ? ? ? ? ? ern.edu ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Subject ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? [Histonet] Ventana vs Leica ?? ? ? ? ? ? 08/24/2010 02:30 ?? ? ? ? ? ? PM ?? ? I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. ?I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. ?If this is you, could you please tell me which you preferred and why. ?? ? I'm currently working for a facility in MT which has narrowed down its search to these two instruments. ?No vendors please, they've already given their pitches. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Thanks, ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. ? ? ? ?Does IHC and ISH (yes- so does the Ventana) 2. ? ? ? Continuous feed (Ventana does NOT offer this!!!!) 3. ? ? ? Space saver, much smaller footprint than Benchmark 4. ? ? ? No wasted antibodies... 5. ? ? ? Not forced to buy EXPENSIVE prep kits either.... 6. ? ? ? Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. ? ? ? Won't blow tissue off the slide! 8. ? ? ? No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, ?I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 ?? ? ? ? ? ? Jay Lundgren ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? To ?? ? ? ? ? ? Sent by: ? ? ? ? ? ? ? ? ?histonet ?? ? ? ? ? ? histonet-bounces@ ? ? ? ? ?? ? ? ? ? ? lists.utsouthwest ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?cc ?? ? ? ? ? ? ern.edu ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Subject ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? [Histonet] Ventana vs Leica ?? ? ? ? ? ? 08/24/2010 02:30 ?? ? ? ? ? ? PM ?? ? I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. ?I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. ?If this is you, could you please tell me which you preferred and why. ?? ? I'm currently working for a facility in MT which has narrowed down its search to these two instruments. ?No vendors please, they've already given their pitches. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Thanks, ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. 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From patrick.lewis <@t> seattlechildrens.org Tue Aug 24 16:50:13 2010 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Tue Aug 24 16:50:25 2010 Subject: [Histonet] Quick frozen sectioning and IMHC questions Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C05814628@s107.childrens.sea.kids> Hi everyone, I cut my first frozen sections, human tonsil, and I have stored them at -70. When I take them out to use, should I bring them to -20C and fix them right away? Or should I bring them to room temp and then pap pen/wash/block/wash them then fix them before adding my primary anti-body. My original plan was/is to bring them to room temp, pap pen them and then wash/block/wash fix. I am asking for your advice as my boss is away for two weeks and the protocol he left me with is a bit vague and requires some modifications to fit the research that we are doing. I was just wondering if there is a compelling reason to keep the sections at/near -20 after sectioning up through the fixation before starting the Immunohistochemistry. Also, we are using BSA as one of the components in our blocking solution as well as a component in our wash solution. Is there a danger in using lower/higher quality BSA? I'm a little worried that our BSA might contribute to increased background if its quality is not high enough. The BSA we may use is A-8327 Sigma, and I am thinking this might be better A3294, or one of these (A9647,A7906,A6793) This is a new job for me, and its been a long while since I have done any Immunohistochemistries.so I am going over the reagents we have on hand while I refresh my memory on immunohistochemistry and sectioning techniques. Thanks for all your support Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From patrick.lewis <@t> seattlechildrens.org Tue Aug 24 17:28:57 2010 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Tue Aug 24 17:29:08 2010 Subject: [Histonet] Another quick sectioning question Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C0581466A@s107.childrens.sea.kids> When I was cutting my block I noticed some ribboning of the tissue. Is there a known cause (or several) for this. I am wondering if my microtome blade was too tight or too loose or if my block froze poorly, or if I just needed to reorient my block . I was cutting 7 um at -20. Its human tonsil, It's been a long time since I used a cryostat (we're using a leicaCM3000). The ribboning almost disappeared when I changed blades after cutting about 30 sections into the block. But I still had some ribboning of the tissue even though I was getting pretty clean OCT slices away from the tissue. Also, anyone have advice on disinfecting cryostats. I will eventually be cutting infectious tissues, HIV human samples and NHP macaques. We plan to follow Leicas disinfection procedure in which you bring the cryostat to Room temp then spray with 5% Bleach let it sit for 10-20 minutes then wipe and spray with 70% ethanol and sit for 10 min then wipe and spray with 100% ethanol. And drain and wipe. Anyone have advice on cleaning and if it's necessary to remove any portions of the cryostat to facilitate cleaning. I figure it will be a two to three day process. To bring it to room temp, clean it, then bring it back down to -20. My main concerns are making sure its disinfected, and then making sure we remove all the bleach so that we don't get corrosion. Thanks for all your help and advice. Patrick. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From gayle.callis <@t> bresnan.net Tue Aug 24 17:57:56 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Aug 24 17:58:09 2010 Subject: [Histonet] Quick frozen sectioning and IMHC questions In-Reply-To: <7EA5752B2903B143A5B845DEA87D5D1C05814628@s107.childrens.sea.kids> References: <7EA5752B2903B143A5B845DEA87D5D1C05814628@s107.childrens.sea.kids> Message-ID: <001101cb43df$cc3403c0$649c0b40$@callis@bresnan.net> Patrick, Bring sections out of freezer and air dry at RT immediately, preferably lay on a countertop to warm as quickly as possible. They may look a little "frosty" but let them warm up for a few minutes - what you see is some water condensation. You cannot wash these sections in buffer before fixation, so mark with PAP pen then fix the air dried sections. After that, go to buffer and then stain. IF you wash before fixation, you will lose an unfixed frozen section off the slide. We prefer to air dry unfixed fresh tissue frozen sections (I presume that is what you did here?) for a minimum of 30 minutes, then put into a storage box that holds ONE days staining run. We put a little bag of 16 mesh silica gel in the box with sections. Take box from freezer, and let the sections come to room temperature for 20 minutes or longer. Open box, mark with PAP pen, fix and stain. If you open the box immediately after removing from freezer, you will get water condensation. And if you open a box, remove some sections, then return box to freezer, you get a thaw/freeze of sections. Water condensation can damage sections and thaw/freeze will eventually damage antigens - I would avoid both. If storing fresh tissue frozen sections as positive and negative controls, use the 5 slide mailers with lids, and take out a mailer to warm up to RT. This keeps other sections frozen until use. We put the mailers w/ slides in a zip lock baggie for quick removal of a few sections to avoid opening and closing a large box. As for BSA, we no longer use it for any immunostaining procedures/buffers/diluents,blocking reagents, etc but if you must use it, there is Jackson's immunoglobulin/protease free. This is also available from Sigma, high quality for molecular biology procedures and in larger quantity. We use only Tween 20 in our buffers now. There is a publication (which I would be happy to send in pdf) about the effects of BSA with primary and secondary antibodies in AIMM journal and why it causes IHC problems. We prefer a normal serum block matched to host of secondary antibody. Once sections are at RT, they stay at RT unless fixed in cold acetone (4C for 10 min, then air dry again at RT) then all staining, etc is done at RT. We never store a frozen section in a cryostat, it goes from pick up to RT for air drying then freezer storage (-80C, -70C is good). Have fun on your new job. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Tuesday, August 24, 2010 3:50 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Quick frozen sectioning and IMHC questions Hi everyone, I cut my first frozen sections, human tonsil, and I have stored them at -70. When I take them out to use, should I bring them to -20C and fix them right away? Or should I bring them to room temp and then pap pen/wash/block/wash them then fix them before adding my primary anti-body. My original plan was/is to bring them to room temp, pap pen them and then wash/block/wash fix. I am asking for your advice as my boss is away for two weeks and the protocol he left me with is a bit vague and requires some modifications to fit the research that we are doing. I was just wondering if there is a compelling reason to keep the sections at/near -20 after sectioning up through the fixation before starting the Immunohistochemistry. Also, we are using BSA as one of the components in our blocking solution as well as a component in our wash solution. Is there a danger in using lower/higher quality BSA? I'm a little worried that our BSA might contribute to increased background if its quality is not high enough. The BSA we may use is A-8327 Sigma, and I am thinking this might be better A3294, or one of these (A9647,A7906,A6793) This is a new job for me, and its been a long while since I have done any Immunohistochemistries.so I am going over the reagents we have on hand while I refresh my memory on immunohistochemistry and sectioning techniques. Thanks for all your support Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com From Marilyn.A.Weiss <@t> kp.org Tue Aug 24 18:02:43 2010 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Tue Aug 24 18:04:10 2010 Subject: [Histonet] I will be out of office beginning the afternoon of 8/23 and returning 8/31 4 2010 and returning 8/13/2010 Message-ID: I will be out of the office starting 08/23/2010 and will not return until 08/31/2010. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. From Bill.Tench <@t> pph.org Tue Aug 24 18:07:09 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Tue Aug 24 18:07:16 2010 Subject: [Histonet] preparation of frozen sections Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A554F@MAIL1.pph.local> So as a pathologist, i have to ask you why you would want to air dry a section? From a diagnostic perspective, we consider air dried samples unacceptable in my lab. All of our standard histologic interpretation is based on fixed sections. So, why not drop the slide in a jar or ETOH and keep it there until you are ready to stain? Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From rsrichmond <@t> gmail.com Tue Aug 24 19:31:40 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Aug 24 19:31:44 2010 Subject: [Histonet] Re: Alcian Yellow Message-ID: Jennifer Johnson asks: >>Can I get someone to share their source for Alcian Yellow? Our Pathologist wants to try a different method for Helicobacter and I need powdered AY? I'd suggest you look into Anatech's method, which bypasses Alcian yellow. (I have no connection with Anatech.) Bob Richmond Samurai Pathologist Knoxville TN From DianaRip1 <@t> aol.com Tue Aug 24 19:47:31 2010 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Tue Aug 24 19:47:44 2010 Subject: [Histonet] PMS2 Message-ID: Can anyone share their protocol for PMS2? I just keep getting background staining. From clb1158 <@t> yahoo.com Tue Aug 24 19:57:55 2010 From: clb1158 <@t> yahoo.com (C B) Date: Tue Aug 24 19:57:58 2010 Subject: [Histonet] Technovit 9100 New Message-ID: <715563.69133.qm@web114017.mail.gq1.yahoo.com> Anyone using Technovit 9100 for microtome and/or ground sections?? Do you use the routine Plus slides for mounting microtome sections or do the sections require another type of adhesive?? When staining the ground sections,??do you have any problems with certain solutions causing cracking/crazing? Cindy Baranowski, HT (ASCP) Saint Joseph's Translational Research Institute Atlanta, Ga From gayle.callis <@t> bresnan.net Tue Aug 24 20:26:38 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Aug 24 20:26:51 2010 Subject: [Histonet] preparation of frozen sections In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A554F@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A554F@MAIL1.pph.local> Message-ID: <000701cb43f4$92678490$b7368db0$@callis@bresnan.net> I can understand your question from the clinical point of view where you want to cut the section, fix, stain and then examine for immediate diagnosis. Not everyone does this. There are many of us, both in clinical and research, who do frozen sections for other than diagnostic reasons. We often need to do immunofluorescent or enzyme immunohistochemical staining (chromogenic) for antigens e.g. CD4, CD8 and many others that will not withstand the kind of fixation you describe. Often we need to do cold acetone fixation or some other solvent fixation for this purpose. In that case, our histologic interpretation is based on a different handling and fixation of a fresh tissue frozen section, and in our case, we cannot just cut and immerse into alcohol. One, the fixative e.g. alcohol is not going to work for our antigen Two, we perform cryomicrotomy on a piece of tissue, collecting as many as a hundred sections, often serial and store the sections until staining (immunostaining in particular) can be performed. Air drying is a form of fixation, and the act of picking up a section onto a slide has been referred to as "flash drying". The antigens we need to see are better when air dried overnight, then fixed with either acetone or acetone/alcohol (in the case, for murine CD markers). Often air drying the section at RT and then fixing with acetone, and storing these fixed sections in a -80C freezer is very acceptable. If I were in a clinical setting and doing a routine H&E, I probably would do exactly as you do now. It is a matter of application. In our case, immersion immediately into a fixative is not optimal for our immunofluorescent or enzyme immunohistochemical results. If we want to see or identify where we are in a sample, we do exactly as you do, section and immerse into fixative, and then do a rapid H&E stain when we can or within a few minutes. We frequently immerse a frozen section into neutral buffered formalin and fix later in the day, week or whenever to have excellent morphology and staining results with an H&E. I hope this clarifies some parameters of performing cryotomy and staining versus how you do it. Gayle M. Callis HTL/HT/MTA(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Tuesday, August 24, 2010 5:07 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] preparation of frozen sections So as a pathologist, i have to ask you why you would want to air dry a section? From a diagnostic perspective, we consider air dried samples unacceptable in my lab. All of our standard histologic interpretation is based on fixed sections. So, why not drop the slide in a jar or ETOH and keep it there until you are ready to stain? Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com From ratliffjack <@t> hotmail.com Tue Aug 24 21:36:53 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Aug 24 21:37:29 2010 Subject: [Histonet] Technovit 9100 New In-Reply-To: <715563.69133.qm@web114017.mail.gq1.yahoo.com> References: <715563.69133.qm@web114017.mail.gq1.yahoo.com> Message-ID: Cindy, I have very little experience with the Technovit kits as I predominantly use a MMA + DBP + Perkadox formulation for both thin and thick section histology. However, in my experience with the Technovit kits, I have discovered for me that the MMA + DBP + Perkadox type of formulation is more consistent, reproducible and overall more flexible for all types of microtomy applications and also for the variety of species (mouse to human) of bone tissue. I have more control over the quality of resin block I can produce with an MMA + DBP + Perkadox formulation, thin sections can be deplastified and staining is more routine and flexible. As for mounting thin microtomed sections, you need to coat your slides with some type of adhesive so that your sections will stay mounted throughout staining. I use Haupt's adhesive to coat my glass slides, along with an aluminum slide press and oven to activate the Haupt's media and complement the adhesion process. There is a step by step process that I can share with you if interested to help accomplish the section adhesion. Now with regards to cracking/crazing of ground (thick) sections in the presence of certain stains. I believe the best way to describe this is that these experiences are proportional to the density or hardness of the resin. If you have a hard resin section (based upon the overall hardness of you resin block), some chemicals will act to make the section brittle and crack similar to prolonged use of xylenes with undeminerized bone processing or prolonged paraffin infiltration is a soft tissue application. Jack On Aug 24, 2010, at 7:57 PM, C B wrote: > Anyone using Technovit 9100 for microtome and/or ground sections? Do you use > the routine Plus slides for mounting microtome sections or do the sections > require another type of adhesive? When staining the ground sections, do you > have any problems with certain solutions causing cracking/crazing? > Cindy Baranowski, HT (ASCP) > Saint Joseph's Translational Research Institute > Atlanta, Ga > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ratliffjack <@t> hotmail.com Tue Aug 24 21:48:15 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Aug 24 21:48:47 2010 Subject: [Histonet] Technovit 9100 New In-Reply-To: <715563.69133.qm@web114017.mail.gq1.yahoo.com> References: <715563.69133.qm@web114017.mail.gq1.yahoo.com> Message-ID: One more thing I might add is that Dorn and Hart Microedge has just released a MMA + DBP + Perkadox kit (Acrylosin) in both a "hard" (ground/thick section formulation) and "soft" (thin section formulation). They are also in the process of carrying a lot of the stains that work well with this type of resin formulation as well as everything in between to assist in resin histology (i.e. slide presses, Haupt's, microtomy supplies/kits, etc). If you haven't already done so, just do an Internet search of their name and you should easily reach their website to view their current products. Please don't hesitate to contact me if you have any additional questions. Jack On Aug 24, 2010, at 7:57 PM, C B wrote: > Anyone using Technovit 9100 for microtome and/or ground sections? Do you use > the routine Plus slides for mounting microtome sections or do the sections > require another type of adhesive? When staining the ground sections, do you > have any problems with certain solutions causing cracking/crazing? > Cindy Baranowski, HT (ASCP) > Saint Joseph's Translational Research Institute > Atlanta, Ga > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From louise.renton <@t> gmail.com Wed Aug 25 01:51:18 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Aug 25 01:51:23 2010 Subject: [Histonet] shrinkage In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1E8D777B9CA@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A1E8D777B9CA@EXC-MBX3.cfs.le.ac.uk> Message-ID: I seem to remember a good discussion in this in the following book:. L. P. Kok and M. E. Boon. *Microwave Cookbook for Microscopists*, Coulomb Press Leyden, Leiden (1992) p. 1?432 . Whetehr or not it is still available is another matter On Tue, Aug 24, 2010 at 5:17 PM, Edwards, Richard E. wrote: > > Anybody aware of the degree of shrinkage in paraffin processed tissues > and/or GMA processed tissues?, many thanks. > > Cheers > Richard Edwards > > Leicester University. > > Leicester U.K. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From settembr <@t> umdnj.edu Wed Aug 25 05:12:36 2010 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Aug 25 05:13:03 2010 Subject: [Histonet] PMS2 Message-ID: Diane, When I was doing PMS2 I was using BD's mouse antibody @ 1:10 Retreived with Dako's TRS in a steamer for 40 min, incubated the antibody for 60 minutes and I used a labelled polymer for the detection (Dako's Envision + Mouse) It was difficult to work up. Good Luck, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> 08/24/10 8:47 PM >>> Can anyone share their protocol for PMS2? I just keep getting background staining. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From clb1158 <@t> yahoo.com Wed Aug 25 08:38:36 2010 From: clb1158 <@t> yahoo.com (C B) Date: Wed Aug 25 08:38:40 2010 Subject: [Histonet] porcine CD31 FFPE Message-ID: <459879.15305.qm@web114004.mail.gq1.yahoo.com> Has anyone used the Serotec # MCA 1746G mouse anti-porcine CD31 or #MCA 1747 mouse anti-porcine CD31 on FFPE porcine arteries?? If so, can you give recommendations for retrieval and dilutions?? The website shows they have "Not determined" reactivity.? Thanks in advance for any recommendations. Cindy Baranowski, HT(ASCP) From chak_bou <@t> yahoo.com Wed Aug 25 08:42:50 2010 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Wed Aug 25 08:42:54 2010 Subject: [Histonet] Lectin From Arachis hypogaea(peanut)- peroxidase Staining Message-ID: <67839.6324.qm@web58105.mail.re3.yahoo.com> Hi Histonet, I am trying to do some staining using Lectin from Arachis hypogaea(peanut)-Peroxidase( FROM SIGMA), and wondering if anyone uses that stain if so, could you please share the protocol with me? Your help will be much appreciated! Thank you Chakib HTL From MIT From Ronald.Houston <@t> nationwidechildrens.org Wed Aug 25 08:45:48 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Aug 25 08:45:59 2010 Subject: [Histonet] Ventana vs Leica In-Reply-To: References: <576316855.263995.1282679277534.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: Bond III hands down; I know Ventana are cutting pricing drastically to get machines in to labs, but the Bond is much more user friendly, especially if you use concentrated antibodies, and also employs the same detection kit for ISH as it does for IHC Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 24, 2010 5:10 PM To: Pamela Marcum; Maria Katleba Cc: histonet; BSullivan@shorememorial.org; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana vs Leica We recently acquired the Bond III. It was a tough decision, but space was the main reason we went with Leica. The Ventana sales team that visited our lab was very eager to get the account. Their pricing was not much different. They even offered to credit us for additional waste disposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pamela Marcum Sent: Tuesday, August 24, 2010 3:48 PM To: Maria Katleba Cc: histonet; BSullivan@shorememorial.org; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica I totally agree and we have an Ultra, 2 XTs and 3 Benchmarks.? They are good systems that need to get cheaper and more flexible.? I am demoing the Bond III and love the ease of use, flexibility and cost savings, most.? Maria is right it is about 40% less to run than a Ventana. Pam Marcum AP Manager UAMS Little Rock, AR ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. ? ? ? ?Does IHC and ISH (yes- so does the Ventana) 2. ? ? ? Continuous feed (Ventana does NOT offer this!!!!) 3. ? ? ? Space saver, much smaller footprint than Benchmark 4. ? ? ? No wasted antibodies... 5. ? ? ? Not forced to buy EXPENSIVE prep kits either.... 6. ? ? ? Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. ? ? ? Won't blow tissue off the slide! 8. ? ? ? No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, ?I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 ?? ? ? ? ? ? Jay Lundgren ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? To ?? ? ? ? ? ? Sent by: ? ? ? ? ? ? ? ? ?histonet ?? ? ? ? ? ? histonet-bounces@ ? ? ? ? ?? ? ? ? ? ? lists.utsouthwest ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?cc ?? ? ? ? ? ? ern.edu ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Subject ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? [Histonet] Ventana vs Leica ?? ? ? ? ? ? 08/24/2010 02:30 ?? ? ? ? ? ? PM ?? ? I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. ?I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. ?If this is you, could you please tell me which you preferred and why. ?? ? I'm currently working for a facility in MT which has narrowed down its search to these two instruments. ?No vendors please, they've already given their pitches. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Thanks, ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. ? ? ? ?Does IHC and ISH (yes- so does the Ventana) 2. ? ? ? Continuous feed (Ventana does NOT offer this!!!!) 3. ? ? ? Space saver, much smaller footprint than Benchmark 4. ? ? ? No wasted antibodies... 5. ? ? ? Not forced to buy EXPENSIVE prep kits either.... 6. ? ? ? Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. ? ? ? Won't blow tissue off the slide! 8. ? ? ? No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, ?I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 ?? ? ? ? ? ? Jay Lundgren ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? To ?? ? ? ? ? ? Sent by: ? ? ? ? ? ? ? ? ?histonet ?? ? ? ? ? ? histonet-bounces@ ? ? ? ? ?? ? ? ? ? ? lists.utsouthwest ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?cc ?? ? ? ? ? ? ern.edu ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Subject ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? [Histonet] Ventana vs Leica ?? ? ? ? ? ? 08/24/2010 02:30 ?? ? ? ? ? ? PM ?? ? I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. ?I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. ?If this is you, could you please tell me which you preferred and why. ?? ? I'm currently working for a facility in MT which has narrowed down its search to these two instruments. ?No vendors please, they've already given their pitches. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Thanks, ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From ree3 <@t> leicester.ac.uk Wed Aug 25 08:49:55 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Wed Aug 25 08:50:00 2010 Subject: [Histonet] shrinkage/a howlong is a piece of string type question Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8D777B9E9@EXC-MBX3.cfs.le.ac.uk> Many thanks to all who responded, for paraffin processed tissues the figures suggested for the amount of shrinkage found or expected were :- "more than 5%":"5-10%":"10%"(twice):"10-15%":"20%":"25%":"30-35%":"30-40%", one responder felt it was "noticeable" and another thought it was a "fairy tale" concocted by pathologists............unsurprisingly many responders thought that the degree of shrinkage was dependent on the fixative used, processing schedule and the nature of the tissue itself, e.g. amount of lipid present. As far as shrinkage with GMA processed tissue go, a single response of "5%" was quoted. Richard Edwards From Maria.Katleba <@t> stjoe.org Wed Aug 25 10:57:19 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Aug 25 10:57:35 2010 Subject: [Histonet] Ventana vs Leica In-Reply-To: References: <576316855.263995.1282679277534.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: Toni brings up a very good point.... you know it's pretty bad when a company is willing to pay your waste costs... Not a good business plan. Why not 'fix' the machine so that it's more "green" Maria Katleba MS HT(ASCP) -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Tuesday, August 24, 2010 2:10 PM To: Pamela Marcum; Maria Katleba Cc: histonet; BSullivan@shorememorial.org; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana vs Leica We recently acquired the Bond III. It was a tough decision, but space was the main reason we went with Leica. The Ventana sales team that visited our lab was very eager to get the account. Their pricing was not much different. They even offered to credit us for additional waste disposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pamela Marcum Sent: Tuesday, August 24, 2010 3:48 PM To: Maria Katleba Cc: histonet; BSullivan@shorememorial.org; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica I totally agree and we have an Ultra, 2 XTs and 3 Benchmarks.? They are good systems that need to get cheaper and more flexible.? I am demoing the Bond III and love the ease of use, flexibility and cost savings, most.? Maria is right it is about 40% less to run than a Ventana. Pam Marcum AP Manager UAMS Little Rock, AR ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. ? ? ? ?Does IHC and ISH (yes- so does the Ventana) 2. ? ? ? Continuous feed (Ventana does NOT offer this!!!!) 3. ? ? ? Space saver, much smaller footprint than Benchmark 4. ? ? ? No wasted antibodies... 5. ? ? ? Not forced to buy EXPENSIVE prep kits either.... 6. ? ? ? Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. ? ? ? Won't blow tissue off the slide! 8. ? ? ? No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, ?I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 ?? ? ? ? ? ? Jay Lundgren ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? To ?? ? ? ? ? ? Sent by: ? ? ? ? ? ? ? ? ?histonet ?? ? ? ? ? ? histonet-bounces@ ? ? ? ? ?? ? ? ? ? ? lists.utsouthwest ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?cc ?? ? ? ? ? ? ern.edu ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Subject ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? [Histonet] Ventana vs Leica ?? ? ? ? ? ? 08/24/2010 02:30 ?? ? ? ? ? ? PM ?? ? I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. ?I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. ?If this is you, could you please tell me which you preferred and why. ?? ? I'm currently working for a facility in MT which has narrowed down its search to these two instruments. ?No vendors please, they've already given their pitches. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Thanks, ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. ? ? ? ?Does IHC and ISH (yes- so does the Ventana) 2. ? ? ? Continuous feed (Ventana does NOT offer this!!!!) 3. ? ? ? Space saver, much smaller footprint than Benchmark 4. ? ? ? No wasted antibodies... 5. ? ? ? Not forced to buy EXPENSIVE prep kits either.... 6. ? ? ? Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. ? ? ? Won't blow tissue off the slide! 8. ? ? ? No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, ?I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 ?? ? ? ? ? ? Jay Lundgren ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? To ?? ? ? ? ? ? Sent by: ? ? ? ? ? ? ? ? ?histonet ?? ? ? ? ? ? histonet-bounces@ ? ? ? ? ?? ? ? ? ? ? lists.utsouthwest ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?cc ?? ? ? ? ? ? ern.edu ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Subject ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? [Histonet] Ventana vs Leica ?? ? ? ? ? ? 08/24/2010 02:30 ?? ? ? ? ? ? PM ?? ? I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. ?I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. ?If this is you, could you please tell me which you preferred and why. ?? ? I'm currently working for a facility in MT which has narrowed down its search to these two instruments. ?No vendors please, they've already given their pitches. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Thanks, ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From gayle.callis <@t> bresnan.net Wed Aug 25 10:59:11 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Aug 25 10:59:25 2010 Subject: Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1E8D777B9E9@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A1E8D777B9E9@EXC-MBX3.cfs.le.ac.uk> Message-ID: <000401cb446e$7768f690$663ae3b0$@callis@bresnan.net> Have you ever thought of doing a shrinkage test? Take a tissue specimen, and xerox or use a flat bed scanner. Put fixed sample between plastic sheets, and scan it as unfixed tissue, fixed before processing and then after processing while in a faced paraffin block. Take all the measurements and then do the calculations./ We used to xerox large stained bone sections, a clever way of getting a precise macro-images of a huge specimen to show gross features of a defect. This did a better job than trying to do a macro-photo with a camera or through a microscope (the latter doesn't happen). Years ago, when preparing for HTL exam practical, the samples e.g. tissue sections submitted had to be within a certain size range, and it was duly noted that after processing, the samples had shrinkage. This required going back to fixed tissue and cutting a bigger piece to compensate for the shrinkage and have a final correct sample/section size to follow the practical rules. As for GMA, there is a special processing schedule given to me that does not use alcohol dehydration (for lipid staining work). This protocol uses an GMA/watergradient since GMA is miscible with water. I would think there would be even less shrinkage with a water/GMA gradient and the source of shrinkage would come from the heat of polymerization and possibly a bit from kind of fixative used. The heat can controlled to some degree by doing polymerization on ice, or in a refrigerator, with the round JB4 metal chucks to dissipate the heat. Once again, I agree with Bryan Hewlett's assessment of shrinkage. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Wednesday, August 25, 2010 7:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shrinkage/a howlong is a piece of string type question Many thanks to all who responded, for paraffin processed tissues the figures suggested for the amount of shrinkage found or expected were :- "more than 5%":"5-10%":"10%"(twice):"10-15%":"20%":"25%":"30-35%":"30-40%", one responder felt it was "noticeable" and another thought it was a "fairy tale" concocted by pathologists............unsurprisingly many responders thought that the degree of shrinkage was dependent on the fixative used, processing schedule and the nature of the tissue itself, e.g. amount of lipid present. As far as shrinkage with GMA processed tissue go, a single response of "5%" was quoted. Richard Edwards _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __________ The message was checked by ESET Smart Security. http://www.eset.com From Jan.Minshew <@t> leica-microsystems.com Wed Aug 25 11:26:38 2010 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Wed Aug 25 11:26:49 2010 Subject: Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question In-Reply-To: <000401cb446e$7768f690$663ae3b0$@callis@bresnan.net> Message-ID: Hey lady, How are you? I haven't seen you on Histonet much lately. I hope that means that you are doing fun things and not working so hard. We have settled in Plano. It's so nice to be around family! Will I see you at NSH? If so, we have to have our night out again so we can catch up on gossip... Kind regards, Jan Minshew Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 Office: 847.405.7051 Cell: 847.970.8468 Fax: 847.405.6560 www.leica-microsystems.com Click Here for this month's special offers! [1]http://www.leica-microsystems.com/bsdspecial "gayle callis" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2010 10:59 AM To "'Edwards, Richard E.'" , cc Subject Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question Have you ever thought of doing a shrinkage test? Take a tissue specimen, and xerox or use a flat bed scanner. Put fixed sample between plastic sheets, and scan it as unfixed tissue, fixed before processing and then after processing while in a faced paraffin block. Take all the measurements and then do the calculations./ We used to xerox large stained bone sections, a clever way of getting a precise macro-images of a huge specimen to show gross features of a defect. This did a better job than trying to do a macro-photo with a camera or through a microscope (the latter doesn't happen). Years ago, when preparing for HTL exam practical, the samples e.g. tissue sections submitted had to be within a certain size range, and it was duly noted that after processing, the samples had shrinkage. This required going back to fixed tissue and cutting a bigger piece to compensate for the shrinkage and have a final correct sample/section size to follow the practical rules. As for GMA, there is a special processing schedule given to me that does not use alcohol dehydration (for lipid staining work). This protocol uses an GMA/watergradient since GMA is miscible with water. I would think there would be even less shrinkage with a water/GMA gradient and the source of shrinkage would come from the heat of polymerization and possibly a bit from kind of fixative used. The heat can controlled to some degree by doing polymerization on ice, or in a refrigerator, with the round JB4 metal chucks to dissipate the heat. Once again, I agree with Bryan Hewlett's assessment of shrinkage. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Wednesday, August 25, 2010 7:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shrinkage/a howlong is a piece of string type question Many thanks to all who responded, for paraffin processed tissues the figures suggested for the amount of shrinkage found or expected were :- "more than 5%":"5-10%":"10%"(twice):"10-15%":"20%":"25%":"30-35%":"30-40%", one responder felt it was "noticeable" and another thought it was a "fairy tale" concocted by pathologists............unsurprisingly many responders thought that the degree of shrinkage was dependent on the fixative used, processing schedule and the nature of the tissue itself, e.g. amount of lipid present. As far as shrinkage with GMA processed tissue go, a single response of "5%" was quoted. Richard Edwards _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __________ The message was checked by ESET Smart Security. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ References 1. http://www.leica-microsystems.com/bsdspecial From llewllew <@t> shaw.ca Wed Aug 25 11:45:28 2010 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Aug 25 11:45:28 2010 Subject: [Histonet] Re: Testing for shrinkage In-Reply-To: <000401cb446e$7768f690$663ae3b0$%callis@bresnan.net> References: <7722595275A4DD4FA225B92CDBF174A1E8D777B9E9@EXC-MBX3.cfs.le.ac.uk> <000401cb446e$7768f690$663ae3b0$%callis@bresnan.net> Message-ID: <2DE191B672A84BF7A50220BF81672A2C@BryanPC> Taking simple measurements of pieces of tissue and then repeating the measurements following fixation, processing and infiltration will certainly allow for estimating the degree of macroscopic shrinkage. Unfortunately, this is not the only shrinkage that takes place, as a simple look at the finished section with a microscope will show. Cells separated from other cells, fibres split off from other fibres, and the like, are all due to microscopic shrinkage and are in addition to the macroscopic shrinkage determined by simple measurement of tissue. I have always presumed that this is why Baker's figures appear to be quite high, because he factored in all forms of shrinkage, not just the obvious. Bryan Llewellyn ----- Original Message ----- From: "gayle callis" To: "'Edwards, Richard E.'" ; Sent: Wednesday, August 25, 2010 8:59 AM Subject: Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question > Have you ever thought of doing a shrinkage test? Take a tissue specimen, > and xerox or use a flat bed scanner. Put fixed sample between plastic > sheets, and scan it as unfixed tissue, fixed before processing and then > after processing while in a faced paraffin block. Take all the > measurements > and then do the calculations./ We used to xerox large stained bone > sections, a clever way of getting a precise macro-images of a huge > specimen > to show gross features of a defect. This did a better job than trying to > do > a macro-photo with a camera or through a microscope (the latter doesn't > happen). > > Years ago, when preparing for HTL exam practical, the samples e.g. tissue > sections submitted had to be within a certain size range, and it was duly > noted that after processing, the samples had shrinkage. This required > going > back to fixed tissue and cutting a bigger piece to compensate for the > shrinkage and have a final correct sample/section size to follow the > practical rules. > > As for GMA, there is a special processing schedule given to me that does > not > use alcohol dehydration (for lipid staining work). This protocol uses an > GMA/watergradient since GMA is miscible with water. I would think there > would be even less shrinkage with a water/GMA gradient and the source of > shrinkage would come from the heat of polymerization and possibly a bit > from > kind of fixative used. The heat can controlled to some degree by doing > polymerization on ice, or in a refrigerator, with the round JB4 metal > chucks > to dissipate the heat. > > Once again, I agree with Bryan Hewlett's assessment of shrinkage. > > Gayle Callis > HTL/HT/MT(ASCP) > Bozeman MT > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, > Richard E. > Sent: Wednesday, August 25, 2010 7:50 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] shrinkage/a howlong is a piece of string type question > > > > Many thanks to all who responded, for paraffin processed tissues the > figures suggested for the amount of shrinkage found or expected were :- > "more than > 5%":"5-10%":"10%"(twice):"10-15%":"20%":"25%":"30-35%":"30-40%", > one responder felt it was "noticeable" and another thought it was a > "fairy > tale" concocted by pathologists............unsurprisingly many responders > thought that the degree of shrinkage was dependent on the fixative > used, > processing schedule and the nature of the tissue itself, e.g. amount of > lipid present. As far as shrinkage with GMA processed tissue go, a > single > response of "5%" was quoted. > > Richard Edwards > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > __________ Information from ESET Smart Security, version of virus > signature > database 5394 (20100824) __________ > > The message was checked by ESET Smart Security. > > http://www.eset.com > > > > > __________ Information from ESET Smart Security, version of virus > signature > database 5394 (20100824) __________ > > The message was checked by ESET Smart Security. > > http://www.eset.com > > > __________ Information from ESET Smart Security, version of virus > signature > database 5396 (20100825) __________ > > The message was checked by ESET Smart Security. > > http://www.eset.com > > > > __________ Information from ESET Smart Security, version of virus > signature > database 5396 (20100825) __________ > > The message was checked by ESET Smart Security. > > http://www.eset.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Ronald.Houston <@t> nationwidechildrens.org Wed Aug 25 12:09:56 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Aug 25 12:10:02 2010 Subject: [Histonet] pituitary block or microarrays Message-ID: Can anyone help with a pituitary block (containing both adeno- and neurohypophysis) or point me in the direction of pituitary microarrays (would need 6 slides)? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From bhewlett <@t> cogeco.ca Wed Aug 25 12:16:51 2010 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Wed Aug 25 12:17:07 2010 Subject: [Histonet] Re: Testing for shrinkage References: <7722595275A4DD4FA225B92CDBF174A1E8D777B9E9@EXC-MBX3.cfs.le.ac.uk><000401cb446e$7768f690$663ae3b0$%callis@bresnan.net> <2DE191B672A84BF7A50220BF81672A2C@BryanPC> Message-ID: Hi Bryan, Good to hear from you! How are you keeping? You are quite correct regarding Baker's data. He engages in some discussion on this matter, mentioning the microscopic shrinkage and gives data on both whole cell volumes as well as nuclear volumes. I don't find his figures high but actually very accurate, in fact in my experience many clinical labs have much higher shrinkage factors than Baker's due to non-optimized processing. But then that doesn't surprise old codgers like us does it? Cheers, Bryan ----- Original Message ----- From: "Bryan Llewellyn" To: "Histonet" Sent: Wednesday, August 25, 2010 12:45 PM Subject: [Histonet] Re: Testing for shrinkage > Taking simple measurements of pieces of tissue and then repeating the > measurements following fixation, processing and infiltration will > certainly allow for estimating the degree of macroscopic shrinkage. > Unfortunately, this is not the only shrinkage that takes place, as a > simple look at the finished section with a microscope will show. Cells > separated from other cells, fibres split off from other fibres, and the > like, are all due to microscopic shrinkage and are in addition to the > macroscopic shrinkage determined by simple measurement of tissue. > > I have always presumed that this is why Baker's figures appear to be quite > high, because he factored in all forms of shrinkage, not just the obvious. > > Bryan Llewellyn > > > ----- Original Message ----- > From: "gayle callis" > To: "'Edwards, Richard E.'" ; > > Sent: Wednesday, August 25, 2010 8:59 AM > Subject: Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a > piece of string type question > > >> Have you ever thought of doing a shrinkage test? Take a tissue specimen, >> and xerox or use a flat bed scanner. Put fixed sample between plastic >> sheets, and scan it as unfixed tissue, fixed before processing and then >> after processing while in a faced paraffin block. Take all the >> measurements >> and then do the calculations./ We used to xerox large stained bone >> sections, a clever way of getting a precise macro-images of a huge >> specimen >> to show gross features of a defect. This did a better job than trying to >> do >> a macro-photo with a camera or through a microscope (the latter doesn't >> happen). >> >> Years ago, when preparing for HTL exam practical, the samples e.g. tissue >> sections submitted had to be within a certain size range, and it was duly >> noted that after processing, the samples had shrinkage. This required >> going >> back to fixed tissue and cutting a bigger piece to compensate for the >> shrinkage and have a final correct sample/section size to follow the >> practical rules. >> >> As for GMA, there is a special processing schedule given to me that does >> not >> use alcohol dehydration (for lipid staining work). This protocol uses an >> GMA/watergradient since GMA is miscible with water. I would think there >> would be even less shrinkage with a water/GMA gradient and the source of >> shrinkage would come from the heat of polymerization and possibly a bit >> from >> kind of fixative used. The heat can controlled to some degree by doing >> polymerization on ice, or in a refrigerator, with the round JB4 metal >> chucks >> to dissipate the heat. >> >> Once again, I agree with Bryan Hewlett's assessment of shrinkage. >> >> Gayle Callis >> HTL/HT/MT(ASCP) >> Bozeman MT >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, >> Richard E. >> Sent: Wednesday, August 25, 2010 7:50 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] shrinkage/a howlong is a piece of string type >> question >> >> >> >> Many thanks to all who responded, for paraffin processed tissues the >> figures suggested for the amount of shrinkage found or expected were :- >> "more than >> 5%":"5-10%":"10%"(twice):"10-15%":"20%":"25%":"30-35%":"30-40%", >> one responder felt it was "noticeable" and another thought it was a >> "fairy >> tale" concocted by pathologists............unsurprisingly many >> responders >> thought that the degree of shrinkage was dependent on the fixative >> used, >> processing schedule and the nature of the tissue itself, e.g. amount of >> lipid present. As far as shrinkage with GMA processed tissue go, a >> single >> response of "5%" was quoted. >> >> Richard Edwards >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> __________ Information from ESET Smart Security, version of virus >> signature >> database 5394 (20100824) __________ >> >> The message was checked by ESET Smart Security. >> >> http://www.eset.com >> >> >> >> >> __________ Information from ESET Smart Security, version of virus >> signature >> database 5394 (20100824) __________ >> >> The message was checked by ESET Smart Security. >> >> http://www.eset.com >> >> >> __________ Information from ESET Smart Security, version of virus >> signature >> database 5396 (20100825) __________ >> >> The message was checked by ESET Smart Security. >> >> http://www.eset.com >> >> >> >> __________ Information from ESET Smart Security, version of virus >> signature >> database 5396 (20100825) __________ >> >> The message was checked by ESET Smart Security. >> >> http://www.eset.com >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Rcartun <@t> harthosp.org Wed Aug 25 13:01:29 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Aug 25 13:01:36 2010 Subject: [Histonet] PMS2 In-Reply-To: References: Message-ID: <4C752238.7400.0077.1@harthosp.org> I don't know whose antibody you are using, but Leica-Microsystems recently released a PMS2 mAb. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 8/24/2010 8:47 PM >>> Can anyone share their protocol for PMS2? I just keep getting background staining. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Wed Aug 25 13:03:56 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Aug 25 13:04:01 2010 Subject: [Histonet] pituitary block or microarrays Message-ID: <20100825110356.9e2d9aa830e8449a2412eb1e4f2f067e.25609f57db.wbe@email04.secureserver.net> I get all my arrays from Biochain. They seem to be pretty e deal with and their quality is hands down better than some other pla ces I have used =) I looked for your pituitary and it said you woul Sarah Goebel, B.A., Histotechnician < XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, T (512)386-5107 < -------- Original Message -------- Subject: [Histonet] pituitary block or microarrays From: "Houston, Ronald" <[1]Ronald.Houston@nationwidechildrens.org> Date: Wed, August 25, 2010 10:09 am To: histonet <[2]histo "[3]ihcrg@googlegroups.com" < Can anyone help with a pituitary block (containing both adeno- and neurohyp microarrays (would need Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital [5]www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 [6]ronald.houston@na tionwidechildrens.org<[7]mailto:ronald.houston@nationwidechildrens.org > [8]www.NationwideChildrens.org< Childrens.org> "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list [10]Histonet@lists.utsouth [11]http: References 1. 3D"mailto:Ronald.Houston@nationwidechil 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:ihcrg@googlegroups.com" 4. 3D"mailto:ihcrg@googlegroups.com" 5. 3D"http://www.childlab.com"/ 6. 3D"mailto:ronald.houston@nationwidechildrens.org" 7. 3D"mailto:ronald.houston@nationwidechi 8. 3D"http://www.NationwideChildrens.org"/ 9. 3D"http://www.NationwideChildrens.org"/ 10. 3D"mailto:Histonet@lists.utsouthwestern.edu" 11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From cevanish <@t> mont-hosp.com Wed Aug 25 13:13:50 2010 From: cevanish <@t> mont-hosp.com (Chris Evanish) Date: Wed Aug 25 13:14:33 2010 Subject: [Histonet] Re: Histonet Digest, Vol 79, Issue 38 In-Reply-To: <20100629174419205@smtp420.redcondor.net> References: <20100629174419205@smtp420.redcondor.net> Message-ID: <4C75251D.6034.00E2.0@mont-hosp.com> Help!! I use a Ventana Benchmark XT. Recently I have gotten a brown granular staining of cytoplasm, not as dark as true specific staining. - Apocrine metaplasic cells. - Non-specifically staining neoplastic prostatic epithelial cytoplasm. Benign prostatic cytoplasm on prostate bx using CK 34BETA12, but not just that antibody as it also appeared in breast tissue on ER. At first I considered it was Endogenous Biotin, so I ran multiple prostate biopsy slides with a blocker but they did not improve. However a strange thing occurred on that run. On two of the slides, one section of tissue stained good, but another section of tissue on the same slide did not stain well. I hope maybe somebody has an idea or two that will help. Thanks, Chris Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email " to your outgoing mail. >>> 6/29/2010 1:44 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Canine bone sectioning trouble (Schneider,Lynda S) 2. RE: Coverslips (Malika Benatti) ---------------------------------------------------------------------- Message: 1 Date: Tue, 29 Jun 2010 12:17:58 -0400 From: "Schneider,Lynda S" Subject: [Histonet] Canine bone sectioning trouble To: "histonet@lists.utsouthwestern.edu" Message-ID: <94A0BFCE84CB0F4A84C50EAC9B02D0A9019423208F@HSC-CMS01.ad.ufl.edu> Content-Type: text/plain; charset="us-ascii" Hello out there... We are having trouble sectioning canine bone. The samples are bone marrow cubes approximately 2cm thick and 1in wide. They were fixed for 15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid decalcifier. They were faced in and then surface decaled again for about 30 mins. When sectioned, much of the marrow was missing and the bone was torn and shredded. We thought that maybe the samples had not been fixed sufficiently so refixed overnight in 10% NBF again. The samples were then reprocessed as they had been originally. The processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each, paraffin x 4, 40 mins each. Again they were faced in and decaled for another 30 mins or so. This time as soon as the sections are placed on the water bath (38 degrees) they explode and/or come apart so severely sections can almost not be picked up. If sections are even obtainable they are of horrible quality. Does anyone have any suggestions? Thank you so much in advance! Lynda ------------------------------ Message: 2 Date: Tue, 29 Jun 2010 17:53:26 +0100 From: "Malika Benatti" Subject: [Histonet] RE: Coverslips To: Message-ID: <4C2A3319.4626.0038.0@gosh.nhs.uk> Content-Type: text/plain; charset=UTF-8 ** Proprietary ** ** Reply Requested When Convenient ** Hi there, We use the CellPath CoverSlips 22x50mm No 1.5 they are great for hand mounting but when used with the Leica CV5030 they are a real pain. most of the CoversSlips get rejected. If anyone use the same equipment, let me know how you are getting one . Cheers, Malika ------------------------------ Message: 7 Date: Mon, 28 Jun 2010 15:06:08 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: Coverslips To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C93@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 8 Date: Mon, 28 Jun 2010 12:21:33 -0700 From: "Kathleen Boozer" Subject: Re: [Histonet] Coverslips To: "histonet@lists.utsouthwestern.edu" , "Joyce Weems" Message-ID: <4C2893CC.4AA8.00C0.1@ah.org> Content-Type: text/plain; charset=US-ASCII I am having the same issues and we purchased the expensive "Platinum" to avoid that problem. They just sent me replacements and I haven't tested them all yet. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk >>> "Weems, Joyce" 6/28/2010 11:28 AM >>> For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 28 Jun 2010 15:36:22 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] RE: Coverslips To: "Weems, Joyce" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2441E@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" We have been using them (24x50) and have had no problems with broken glass pieces. I do agree that they seem "dirty". Never contacted them about it. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 3:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Coverslips I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 10 Date: Mon, 28 Jun 2010 12:41:44 -0700 From: sgoebel@xbiotech.com Subject: RE: [Histonet] RE: Coverslips To: "Weems,Joyce" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <20100628124144.9e2d9aa830e8449a2412eb1e4f2f067e.fdd3de83db.wbe@email04.secureserver.net> Content-Type: text/plain; charset="utf-8" I just got a box of these as a "trial". They all have been far (used 1/2 the box so far)? Maybe there is gunk in your co verslipper that is causing this or possibly some glass particles? I c Sarah Goebel, B.A., HT (ASCP) < Histotechnicia XBiotech USA Inc. < Austin, Texas 78744 < (512) -------- Original Message -------- Subject: [Histonet] RE: Coverslips From: "Weems, Joyce" <[1]JWeems@sjha.o Date: Mon, June 28, 2010 12:06 pm To: "[2]histonet@lists. <[3]histonet@lists.u I forgot to say that the packaging has changed to their Histo Hippo theme, -----Original Message----- From: [4]histon [[5]mailto:histonet-bounces@lists.utsouthwestern.edu Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: [6]histonet@lists.u Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white with them air bubbles when us having this problem? They and have replaced several boxes, same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health E recipient(s). It may contain information that is privileged and confidential. Any unauth If you are n and reply to the sen _______________________________________________ Histonet mailing list [7]Histonet@lists.utsou [8]http: Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list [9]Histonet@lists.utsou [10]http: References 1. 3D"mailto://JWeems@sjha.org"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 4. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/ 5. 3D"mailto:histonet-bounces 6. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 7. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 8. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 9. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" ------------------------------ Message: 11 Date: Mon, 28 Jun 2010 14:48:44 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] Tissue Processors To: "'Feher, Stephen'" , mohamed abd el razik , "Histonet@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2CC@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="iso-8859-1" I second Steve's comments about the Peloris. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, June 28, 2010 1:56 PM To: mohamed abd el razik; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processors We have 2 Peloris processors and run protocols ranging from 2 hrs to overnight. Several 2 and 4 hour protocols daily keeps a constant flow of specimens moving through the lab for those interested in LEAN small batch processing. As far as programmability, it 's all touch screen driven and easy. It's a smart processor so we don't change all solutions weekly. We only change the ones that are needed. The parameters that determine how long to use a particular solution are also programmable. Changing solutions is done by pumping out of the individual bottles and into waste containers and refilling is pumped from clean containers of solution back into the original containers. No heavy lifting required. Ours are in a separate room from our microtomy area so we hooked a simple door bell up to the local alarm jack on the back. When processing is done, we get the appropriate tone. Remote alarm is also tied in to the hospital switchboard in case a processor goes down at night or during the weekend. We really like the versatility and dependability of these processors. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Saturday, June 26, 2010 3:41 PM To: Histonet@lists.utsouthwestern.edu Subject: Fw: [Histonet] Tissue Processors i need it too as we are going to bring new tissue processor to our small lab --- On Sat, 6/26/10, Shirley Pan wrote: From: Shirley Pan Subject: [Histonet] Tissue Processors To: histonet@lists.utsouthwestern.edu Date: Saturday, June 26, 2010, 7:55 AM We are in the process of trying out tissue processors. Are there any users of the Leica Peloris or Thermo EG who can help us out with some opinions? Reliability, ease of changing solutions, programmability? Thanks for any help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 12 Date: Mon, 28 Jun 2010 16:04:27 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] RE: Coverslips To: "sgoebel@xbiotech.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DFD5CBA@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We can coverslip by hand, but soon after the slides are coverslipped on the machine, air bubbles begin to form. We we coverslip them by hand, there is visible glass that we can feel and move if we need too. They've been good for so long, I want to make sure it's not just us. Thanks for the feedback! j ________________________________ From: sgoebel@xbiotech.com [mailto:sgoebel@xbiotech.com] Sent: Monday, June 28, 2010 15:42 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Coverslips I just got a box of these as a "trial". They all have been fine so far (used 1/2 the box so far)? Maybe there is gunk in your coverslipper that is causing this or possibly some glass particles? I coverslip by hand. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] RE: Coverslips From: "Weems, Joyce" > Date: Mon, June 28, 2010 12:06 pm To: "histonet@lists.utsouthwestern.edu" > I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 13 Date: Mon, 28 Jun 2010 13:19:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Unstained Slides To: histonet@lists.utsouthwestern.edu, Rick.Garnhart@memorialhealthsystem.com Message-ID: <946724.39272.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Depending on the??epitope you are trying to detect, the time could be limited??from less than one month to 1-2 years. Ren?? J. --- On Mon, 6/28/10, Rick.Garnhart@memorialhealthsystem.com wrote: From: Rick.Garnhart@memorialhealthsystem.com Subject: [Histonet] Unstained Slides To: histonet@lists.utsouthwestern.edu Date: Monday, June 28, 2010, 11:54 AM Histoland, How?? is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph:?? 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 28 Jun 2010 14:58:48 -0600 From: Jay Lundgren Subject: [Histonet] Inexpensive fume hood? To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Can anyone recommend an inexpensive fume hood/ workstation? Not the full on metal type that hangs from the ceiling, but the Lucite bench top unit with a carbon filter. I want to avoid the capital equipment rigmarole, so it has to cost less than $1000. It is to put a small automated coverslipper under, 16" H x 20" W x 10" D . Thanks, Jay A. Lundgren, M.S., HTL (ASCP) ------------------------------ Message: 15 Date: Mon, 28 Jun 2010 14:04:42 -0700 From: Pat Laurie Subject: Re: [Histonet] Tissue Processors To: "Mahoney,Janice A" Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I 3rd the previous statements about the Peloris. I had 2 pelori at my previous job, and now we currently have 3 pelori. We are also looking at getting a 4th. We use them regularly and due to the ability to use shorter programs, we run about 15 runs a day between them while still having significant downtime. I must warn you though, it is necessary to keep the service contracts on them. They are about 99% computer and have a significant number of moving parts, but they do work wonderfully. On Mon, Jun 28, 2010 at 12:48 PM, Mahoney,Janice A < Janice.Mahoney@alegent.org> wrote: > I second Steve's comments about the Peloris. > Jan Mahoney > Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen > Sent: Monday, June 28, 2010 1:56 PM > To: mohamed abd el razik; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Tissue Processors > > We have 2 Peloris processors and run protocols ranging from 2 hrs to > overnight. Several 2 and 4 hour protocols daily keeps a constant flow of > specimens moving through the lab for those interested in LEAN small batch > processing. As far as programmability, it 's all touch screen driven and > easy. It's a smart processor so we don't change all solutions weekly. We > only change the ones that are needed. The parameters that determine how > long to use a particular solution are also programmable. Changing solutions > is done by pumping out of the individual bottles and into waste containers > and refilling is pumped from clean containers of solution back into the > original containers. No heavy lifting required. > > Ours are in a separate room from our microtomy area so we hooked a simple > door bell up to the local alarm jack on the back. When processing is done, > we get the appropriate tone. Remote alarm is also tied in to the hospital > switchboard in case a processor goes down at night or during the weekend. > > We really like the versatility and dependability of these processors. > > > Steve > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el > razik > Sent: Saturday, June 26, 2010 3:41 PM > To: Histonet@lists.utsouthwestern.edu > Subject: Fw: [Histonet] Tissue Processors > > > i need it too as we are going to bring new tissue processor to our small > lab > --- On Sat, 6/26/10, Shirley Pan wrote: > > > From: Shirley Pan > Subject: [Histonet] Tissue Processors > To: histonet@lists.utsouthwestern.edu > Date: Saturday, June 26, 2010, 7:55 AM > > > We are in the process of trying out tissue processors. Are there any users > of the Leica Peloris or Thermo EG who can help us out with some opinions? > Reliability, ease of changing solutions, programmability? Thanks for any > help. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is > faithful to the healing ministry of Jesus Christ, providing high quality > care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is > confidential and private and intended only for the use of the addressees. > Unauthorized use, disclosure, distribution or copying is strictly > prohibited and may be unlawful. If you received this communication in > error, please inform us of the erroneous delivery by return e-mail message > from your computer. Additionally, although all attachments have been > scanned at the source for viruses, the recipient should check any > attachments for the presence of viruses before opening. Alegent Health > accepts no liability for any damage caused by any virus transmitted by this > e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com ------------------------------ Message: 16 Date: Mon, 28 Jun 2010 17:57:37 -0500 From: "Amador, Amanda" Subject: [Histonet] Modified Genta To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <1D9B439FC446244095353DCA4FC23DD20454E1D27B@MWNMAIL00.ameripath.local> Content-Type: text/plain; charset="us-ascii" Is there someone that could help out with the Modified Genta? We tried it in the microwave and we are not sure if we are doing it correctly. We would appreciate any sample procedures to see if we need to change something. Thanks to anyone who can help out. aamador@ameripath.com ------------------------------ Message: 17 Date: Tue, 29 Jun 2010 09:53:10 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] what do you think? To: mohamed abd el razik Cc: Histonet@lists.utsouthwestern.edu Message-ID: <4C29FAC6.1030909@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Greetings Mohamed: The lamina propria brings small blood vessels and lymphatics close to the epithelium lining the large and small intestines. This allows nutrients to be passed back and forth and for the immune system to monitor any antigens that cross the epithelium. The relationship between the immune system and the contents of the GI tract is very complex. Lymph nodules in the lamina propria would make it thicker in some regions than in other regions. Whether the thickening you refer to is pathological or just the normal response to a variety of factors is difficult to say. Geoff mohamed abd el razik wrote: > dear histonetters > i have a quistion please regarding the thickness of lamina propria in small or larg intestine. > what is the significant of it?? does it mean inflamation and pathological condition or mean more size and so absorption of nutrients? i mean does the thickning is favorable or not? > > thanks > Mohamed Abd Elrazik > Histology dep. > Fac. of Vet. Med. > Cairo . Univ. -Egypt > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 18 Date: 29 Jun 2010 13:59:09 -0000 From: "abijag " Subject: [Histonet] Your experience with microm HMS 740 auto stainer To: Message-ID: <1277819686.S.1418.34634.webmail.rediff.com.drafts.1277819949.50235@webmail.rediffmail.com> Content-Type: text/plain; charset="UTF-8" Dear Histonetters, We would like to procure one tissue auto stainer for our histology lab(mainly H&E staining) In this respect, I request your experience about Microm HMS 740 automatic stainer. Please share your comments regarding the staining reproducibility,ease of handling and their claim about drip prevention technology. Based on your valuable feedback, we will make our decision. Thanks for all help Abi jagannath ------------------------------ Message: 19 Date: Tue, 29 Jun 2010 08:20:32 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Inexpensive fume hood? To: histonet , Jay Lundgren Message-ID: <364541.78070.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Check a Fisher catalog. The ones they sell are good for your purposes. Ren?? J. --- On Mon, 6/28/10, Jay Lundgren wrote: From: Jay Lundgren Subject: [Histonet] Inexpensive fume hood? To: "histonet" Date: Monday, June 28, 2010, 4:58 PM Can anyone recommend an inexpensive fume hood/ workstation??? Not the full on metal type that hangs from the ceiling, but the Lucite bench top unit with a carbon filter.?? I want to avoid the capital equipment rigmarole, so it has to cost less than $1000.?? It is to put a small automated coverslipper under,?? 16" H x 20" W x 10" D . ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ??????Thanks, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 29 Jun 2010 08:53:32 -0700 From: "Morken, Tim" Subject: RE: [Histonet] New CAP question ANP.22760 To: "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4C021C3610F1@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Thomas and Daniel both make good points. While clinical labs do run measurable (quantitative) tests they are also running completely closed systems in which all reagents are from a certain vendor. All the reagents are validated by a vendor and all reagents are validated and calibrated for that system. Running qualitative tests is only different at the interpretive stage. All other stages should be standardized and validated to ensure the system works as intended every day, every test. IHC, of course, is a whole different ballgame. There probably is not a single lab out there that uses only one vendor and uses only one system to do IHC testing. Even if you use one automated staining system you may use antibodies from a different vendor, or you may do something outside the system that is very different than other labs use. There is such disparity between testing in different labs that it is difficult to compare results and even techniques between labs. That is the crux of the issue and what CAP and others are trying to address by tightening the validation screws. In the near future labs will need to have very robust validation and documentation for all antibodies in order to prove they are doing things correctly. That scenario is already here for the breast markers. It will come for the others as well. Right now the instances in which solid validation methods are absolutely necessary is when validating the breast markers ER, PR and HER2, which all are used as stand-alone tests to determine treatment, and for ASR's. If you use a vendor kit with all reagents supplied you must still prove it works in your lab as intended ("verify outside data." The "outside data" is the claim that it stains in a certain way). You must run cases of various expressions and show your lab gets the proper results. Third party verification is very helpful, that is, comparing your results to that of other labs on the same material (CAP surveys, or set up a partnership with another lab). If you use only the antibody for one of those markers, or mix and match components outside a FDA-approved kit, then you are responsible for performing a comprehensive validation of the test. Its worth noting that every news article I've seen about pathology/histology labs in the last several years has been about failures to do the breast panel tests correctly. We are under a microscope these days and it will pay off to make sure you are doing the tests well! Most other markers in IHC are ancillary tests that are run in conjunction with other tests to determine a diagnosis. Those antibodies still need to be validated in your system but don't require a large sample of cases. They have been validated as IVD's by the vendor so just need to be shown they work as intended with your lab's methods and instruments. But I still think it is worthwhile to do a validation that includes a range of expressions along with irrelevant tissue (negative). That will help calibrate your expectations about how the antibody works and give you a baseline to refer to if problems arise. Running one or two slides to make sure it "works" is not really satisfactory. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, June 26, 2010 1:45 PM To: Daniel Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Daniel, I think your observations are very good. Which brings me again to a point I was trying to make earlier. Anatomic Pathology is not the same as the General Clinical Lab or it's close counterparts. It almost seems like this is overblown re: validation. As you've pointed out, the interpretation is up to the pathologist. Patients (prostate, i.e.) will correlate with other tests. When known positives demonstrate properly you're valid. Even automated systems can use algorithms to score strength of positivity...which varies yet will show as positive (valid). You receive a new batch of Ab you run it on a known positive, that's the bottom line. It seems somehow CAP or clinical lab (trained) folks want more than that. And again (as you pointed out) the appreciation for the differences in how we operate as laboratorians seems to fall by the wayside. As you mention pushing 20, 50, 100 is not feasible (and frankly unnecessary). I don't know where the answer lies in making everyone happy here. I do know that some thinking outside the box and trying understand AP is required. We all want good patient care, but one size does not fit all. Thanks, tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Sent: Saturday, June 26, 2010 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 One question I think a lot of you are not considering is that the clinical laboratory usually tests for analytes present in most patients. This allows the clinical lab to more easily run validations with hundreds of patients, using statistical tools to analyze the precision, specificity and sensitivity of the test. What most of those concerned with this new CAP standard (and I count among them) is that our testing often targets a very rare population of patients. As such, an extensive validation is much more difficult to establish. It then makes the statistical models used in clinical tests much less valuble since the accuracy of these formulas decrease with smaller sample sizes. Additionally, the pathology of the clinical tests are reported as a measurement which does not have an intrinsic value. It must be interpreted by a clinician to give value to the patient. For example, the Blood Urea Nitrogen test value range usually is between 7-20 mg/dL while values above 50 are considered a marker of poor kidney function. Elevated values, however, do not mean that kidney functions are impaired but instead may be due to other pathologies such as congestive heart failure, gi bleeding, certain steroids and even diet. For histopathology even relatively common antibodies such as pan-T cell marker CD3 usually only stain 75% of T cell neoplasms. A negative result for that minority does not indicate that the tumor does not have a T cell lineage. It relies instead on the pathologist interpreting the result much as the clinician looks at the BUN value in relation to other factors in the patient's presentation. When we validate this kind of antibody, do we perform testing then on T cells and B cells (give me a good tonsil), on malignant samples or some combination of the two? What is a reasonable number of tests to run then? If I choose 10 normal lymphatic tissue blocks for my routine T/B cell marking (it can't be as extensive as ER/PR can it?) how many samples should I choose for my malignant population? If I use another 10 anyone with a background in statistics will tell you that the sample size is too small to interpret accuracy. If I push it up to 20, 50 or 100 I would be certain that many laboratories would not be able to afford the cost of the validation. This would then push many good laboratories out of the business of IHC with the unintended result of delaying diagnoses and increasing patient costs by driving testing to fewer and fewer testing outlets. CAP's new path with ER/PR seems to be trying to achieve a noble end of improving quality in the laboratory but without an understanding of the complexities and consequences of the method they are implementing. Dan -----Original Message----- >From: Jesus Ellin >Sent: Jun 26, 2010 6:28 AM >To: "Morken, Tim" , >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >I have been reading the post to this question and it seems to me that there are different standards depending on the lab that is operating the methodology. I do agree that the core lab for years have had the instruction and training in the performance of validation. One thing that comes to mind as well is why has histology not had this training? Why are we not getting this from our certification agency, our professional societies and biggest reason where is our standardization. It seems to me that with all these regualtions in plac for so long, why were we missed. Is it because when inspected through CAP we are being inspected by a pathologist rather than a histo tech? These are some of the questions at hand. I to see new standards within the CAP checklist as well as other regulatory organizations that will affect the future of the Anatomic Pathology community. But I think we need is to provide a underlying architecture for our peers, so that we can begin the transition to the future. This is only the beginning, there is still Digital Image Analysis and Telepathology. It funny we are looking to become a hybrid of radiology and the core lab, but with the best of both worlds. Tim great structure for the validation study. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, >Tim >Sent: Wed 6/23/2010 9:48 AM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Joe, > >You wrote : The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time ..." > >Those were my thoughts exactly. While the person replying may or may not have specific histology experience she will have clinical lab experience (however, my guess is that she is exposed to histology regularly at CAP). Clinical labs have a bit of an easier time, actually, because they validate primarily to known concentration controls - analytical controls manufactured at a range of known concentrations for instance. The institution then adds in their normal controls for validation. > >As far as the current question about validating a new lot of reagent the best practice is to run parallel tests on the same machine. If that is not easily possible on a particular manufacturer's instrument then the question should be asked of them: Why not? If this is a requirement the manufacturer should provide an easy path to meeting the requirement. However, if that is not the case then the institution simply writes a procedure to get around the inadequacies of the instrument (Maybe the vendor can help with that). Then follow the procedure. That should satisfy inspectors. > >An "...appropriate panel of tissues..." is whatever the institution deems appropriate for the given antibody or reagent. This is a perfect place for tissue arrays. You can make your own or buy them. > >IHC must meet CLIA validation guidelines but since IHC is generally qualitative the requirements must be understood and methods adapted to a qualitative scenario. Several IHC and Histotechnology books discuss the subject at length (Taylor, Dabbs, Bancroft for instance). > >Below is a brief overview of how to do that. (for more in-depth info this was covered in an NSH teleconference I gave last year - PowerPoint, audio and references available from NSH-, and will be covered in a similar workshop at NSH in Seattle this year). > > >1) >CAP General Validation >CAP GEN.42020-42163 Test Method Validation Follows CLIA CFR Sec >493.1253 Does not apply well to IHC (IHC is usually qualitative) > >But the general principle applies: >The laboratory must have data on each test's accuracy, precision, analytic sensitivity, interferences and reportable range. > >Unmodified FDA-cleared or approved tests: the lab may use manufacturer information or published reports but lab must verify outside data. > >Non-FDA cleared: Lab MUST verify or establish analytic accuracy, precision, sensitivity, specificity and reportable range. > >2) Validation includes: >Accuracy: > Compare results with New antibody to a previously validated antibody on the same tissues > >Precision: > Test samples with varying antigen expression > Intra-run, Inter-run tests, 10 slides each (reproducibility) > >Sensitivity: > True Positive vs False Negative (higher % FN = less sensitive) > >Interferences [Specificity]: > True Negative vs False Positive (Higher % FP = less specific) > Delineate what could interfere to give a false positive or false negative result. > >Reportable Range > Establish a scoring system > Provide the definition of a positive result > >3)Sensitivity > >Analytic Sensitivity: > Lowest amount of substance detectable by the test > Can only be done with controls of known concentration > >Diagnostic Sensitivity: > Ability of the test to determine true diagnostic positive verses false negative (higher % FN = less sensitive) > Requires comparison to a previously validated antibody > >IHC Sensitivity: > Extent to which an antibody can be diluted and still achieve target recognition. NOTE: This is determined by antibody AND detection system! > > > >4) Specificity: > >Analytic Specificity > Accuracy on tests of known positive and negative controls > Controls of known concentration > Determine what could "Interfere" to confound the result > > >Diagnostic Specificity > Ability of a test to determine true diagnostic negative verses false positives (Higher % FP = less specific) > Requires comparison to a previously validated antibody > > >IHC Specificity > Ability of an antibody to bind exclusively to its particular antigen in the absence of staining of other molecules > Or, staining of other structures in addition to target >structures/cells > >(Sensitivity and Specificity adapted from: Theoretical and Practical >Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote, 2005) > >Tim Morken >Supervisor, Histology / IPOX >UCSF Medical Center >San Francisco, CA > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >JMyers1@aol.com >Sent: Tuesday, June 22, 2010 6:51 PM >To: tjasper@copc.net >Cc: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Tom: > >As much as I agree with your acknowledgment that its seems a bit odd >for the CAP to have a blood-banker responding to AP-related issue, I'm >actually not surprised. The folks in the 'clinical' lab have been >performing more comprehensive and complex validation procedures for a >very long time, and they wonder why IHC isn't expected to follow the >same requirements as chemistry, immunology, etc. -- IHC is, after all, >an awful lot like ELISA. And rightfully so, because IHC is, under CLIA >(which supersedes CAP), considered highly-complex, non-waived testing >-- and is, therefore, subject to the same Quality Systems regulations >(in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. > >Could it be that, because AP produces qualitative results that are >interpreted by a pathologist and CP produces quantitative results that >are interpreted by an analyzer, we somehow think that CLIA rules don't >apply to IHC? I certainly don't have the answer to that, but it make >me wonder what the future holds. As witnessed by some of the newest >CAP 'standards' (including the question in question...no pun intended), >e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens >must be tested, and where 10 of the positives must be weakly positive >-- an acknowledgment that validation specimens must be carefully >selected in order to obtain appropriate results), it certainly doesn't >appear that the regulation of IHC testing is going to become more relaxed. > >Joe Myers, M.S., CT(ASCP) > >------------------------------ > >Message: 12 >Date: Fri, 18 Jun 2010 12:38:07 -0700 >From: "Thomas Jasper" >Subject: RE: [Histonet] New CAP question ANP.22760 >To: "Mark Tarango" >Cc: _histonet@lists.utsouthwestern.edu_ >(mailto:histonet@lists.utsouthwestern.edu) > >Mark, > >Did you notice the credentials from this CAP representative? MT with a >Blood Bank specialty I believe. What I glean from that is...more than >likely this person does not grasp the logistics of "contemporaneously" >staining identical Abs from separate lots. She also likely does not >understand the logistical application for detection and automation >either. > >I'm not trying to be overly critical of this person. I'm sure she is >quite intelligent and would not have the MT/SBB if she wasn't >intelligent. It comes down to a lack of understanding Anatomic >Pathology testing application re: automated IHC. I believe this is a >common problem in and out of CAP. Many lab directors and other folks in >positions of authority without AP/Histology/Cytology backgrounds seem >to believe that broad clinical lab modalities apply to Anatomic Path >scenarios. I used to refer to this in my former position as - "Trying >to put the yoke of clinical lab onto anatomic path." We are >laboratorians, but in many instances do not fit the general clinical >lab mold. > >It's unfortunate that CAP has put this person in the position to >respond. It is apparent to me that she's not grasping the particulars >here. She probably never will unless she decides to go into a working, >automated IHC "tissue" lab and take the time to ask questions and >understand (learn) what we're all about. > >Thanks, >Tom Jasper > >Thomas Jasper HT (ASCP) BAS >Histology Supervisor >Central Oregon Regional Pathology Services Bend, OR 97701 >_______________________________________________ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 37 **************************************** ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 38 **************************************** From neil.fournier <@t> yale.edu Wed Aug 25 13:42:16 2010 From: neil.fournier <@t> yale.edu (Neil M. Fournier) Date: Wed Aug 25 13:42:20 2010 Subject: [Histonet] pErk/Ki67 doublelabeling Message-ID: <20100825144216.bjzvzl5ha84cgccw@www.mail.yale.edu> I am working with fixed (10% buffered formalin) rat brain sections and I have had some difficulty getting pErk/Ki67 immunofluorescent colabeling to work. I get beautiful Ki67 labeling but absolutely no pErk labeling anywhere. I am using mouse monoclonal pErk (Cell Signaling) and rabbit monoclonal Ki67(Vector) antibodies. Primaries are incubated simultaneously for 48 hr at 4 degree C. I am using Alexa 488 and 546 secondary antibodies, which are always given simultaneously during staining for 3 hr at RT. Monolabeling with pErk is fine with other brain sections (the only difference is that they were sectioned on a freezing microtime versus a vibratome in the present study). However, the only labeling I see with pErk in my sections is nonspecific labeling of capillary beds throughout the section. I have tried staining both with and without citrate buffer antigen retrieval and there is no difference. Does anyone have any suggestions or recommendations? Neil From swongman <@t> yahoo.com Wed Aug 25 15:58:55 2010 From: swongman <@t> yahoo.com (Steve Wong) Date: Wed Aug 25 15:59:41 2010 Subject: [Histonet] Volumetric determination of a lesion in a rat spinal cord Message-ID: <32819.4626.qm@web56201.mail.re3.yahoo.com> Hello, I am looking for a CRO well-versed in the volumetric determination and 3D reconstruction of demyelinated lesions located in the rat spinal cord. Hopefully, also with experience automating the measurement in luxol fast blue stained sections. From Rcartun <@t> harthosp.org Wed Aug 25 18:15:37 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Aug 25 18:15:43 2010 Subject: [Histonet] Re: [IHCRG] PAX-5 - B-cell specific activator protein (BSAP) In-Reply-To: References: <712c5335-e273-4c8c-9e87-54912c941d7a@EXCA2.meriter.com> Message-ID: <4C756BD8.7400.0077.1@harthosp.org> We use a mouse mAb (clone 24) from BD Biosciences on Leica-Microsystems' Bond with absolutely no problems. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Taylor, Jean" 8/19/2010 10:44 AM >>> Hi Everyone, I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP). Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 18, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD34 positive control (McMahon, Loralee A) 2. Ventana Renaissance Containers (ricky hachy) 3. CD34 Control (Silverman, Jeffrey) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger , Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensperger@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensperger@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 18 Aug 2010 16:28:39 +0000 From: ricky hachy Subject: [Histonet] Ventana Renaissance Containers To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello , I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky ------------------------------ Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: "Silverman, Jeffrey" Subject: [Histonet] CD34 Control To: "'jreichensperger@siumed.edu'" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <83F4D81747A7094DAFE3AE87151ECB941CE41442A3@SYKECHXVS01.nslijhs.net> Content-Type: text/plain; charset="us-ascii" Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 **************************************** -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From kmerriam2003 <@t> yahoo.com Thu Aug 26 07:40:28 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Aug 26 07:40:30 2010 Subject: [Histonet] special stain for H. billis Message-ID: <90400.59877.qm@web50306.mail.re2.yahoo.com> Good morning everyone, Does anyone know of a special stain?that is specifically for H.?billis.? I don't know much about bacteria, so I?am not even sure which bacterial stain would work on this.? Any comments would be appreciated. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From pathstaff <@t> brhealthsystem.org Thu Aug 26 09:09:36 2010 From: pathstaff <@t> brhealthsystem.org (Pathology Staff) Date: Thu Aug 26 09:10:02 2010 Subject: [Histonet] RE: Histonet Digest, Vol 81, Issue 33 In-Reply-To: <1477820942873330084066564973764@psmtp.com> Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 25, 2010 12:33 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. I will be out of office beginning the afternoon of 8/23 and returning 8/31 4 2010 and returning 8/13/2010 (Marilyn.A.Weiss@kp.org) 2. preparation of frozen sections (Tench, Bill) 3. Re: Alcian Yellow (Robert Richmond) 4. PMS2 (DianaRip1@aol.com) 5. Technovit 9100 New (C B) 6. RE: preparation of frozen sections (gayle callis) 7. Re: Technovit 9100 New (Jack Ratliff) 8. Re: Technovit 9100 New (Jack Ratliff) 9. Re: shrinkage (louise renton) 10. Re: PMS2 (Dana Settembre) 11. porcine CD31 FFPE (C B) 12. Lectin From Arachis hypogaea(peanut)- peroxidase Staining (Chakib Boussahmain) 13. RE: Ventana vs Leica (Houston, Ronald) 14. shrinkage/a howlong is a piece of string type question (Edwards, Richard E.) 15. RE: Ventana vs Leica (Maria Katleba) 16. Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question (gayle callis) 17. Re: Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question (Jan.Minshew@leica-microsystems.com) ---------------------------------------------------------------------- Message: 1 Date: Tue, 24 Aug 2010 16:02:43 -0700 From: Marilyn.A.Weiss@kp.org Subject: [Histonet] I will be out of office beginning the afternoon of 8/23 and returning 8/31 4 2010 and returning 8/13/2010 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 08/23/2010 and will not return until 08/31/2010. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. ------------------------------ Message: 2 Date: Tue, 24 Aug 2010 16:07:09 -0700 From: "Tench, Bill" Subject: [Histonet] preparation of frozen sections To: Histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A554F@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii So as a pathologist, i have to ask you why you would want to air dry a section? From a diagnostic perspective, we consider air dried samples unacceptable in my lab. All of our standard histologic interpretation is based on fixed sections. So, why not drop the slide in a jar or ETOH and keep it there until you are ready to stain? Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 3 Date: Tue, 24 Aug 2010 20:31:40 -0400 From: Robert Richmond Subject: [Histonet] Re: Alcian Yellow To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Jennifer Johnson asks: >>Can I get someone to share their source for Alcian Yellow? Our Pathologist wants to try a different method for Helicobacter and I need powdered AY? I'd suggest you look into Anatech's method, which bypasses Alcian yellow. (I have no connection with Anatech.) Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 4 Date: Tue, 24 Aug 2010 20:47:31 EDT From: DianaRip1@aol.com Subject: [Histonet] PMS2 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Can anyone share their protocol for PMS2? I just keep getting background staining. ------------------------------ Message: 5 Date: Tue, 24 Aug 2010 17:57:55 -0700 (PDT) From: C B Subject: [Histonet] Technovit 9100 New To: Histonet@lists.utsouthwestern.edu Message-ID: <715563.69133.qm@web114017.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Anyone using Technovit 9100 for microtome and/or ground sections? Do you use the routine Plus slides for mounting microtome sections or do the sections require another type of adhesive? When staining the ground sections, do you have any problems with certain solutions causing cracking/crazing? Cindy Baranowski, HT (ASCP) Saint Joseph's Translational Research Institute Atlanta, Ga ------------------------------ Message: 6 Date: Tue, 24 Aug 2010 19:26:38 -0600 From: "gayle callis" Subject: RE: [Histonet] preparation of frozen sections To: "'Tench, Bill'" , Message-ID: <000701cb43f4$92678490$b7368db0$@callis@bresnan.net> Content-Type: text/plain; charset="US-ASCII" I can understand your question from the clinical point of view where you want to cut the section, fix, stain and then examine for immediate diagnosis. Not everyone does this. There are many of us, both in clinical and research, who do frozen sections for other than diagnostic reasons. We often need to do immunofluorescent or enzyme immunohistochemical staining (chromogenic) for antigens e.g. CD4, CD8 and many others that will not withstand the kind of fixation you describe. Often we need to do cold acetone fixation or some other solvent fixation for this purpose. In that case, our histologic interpretation is based on a different handling and fixation of a fresh tissue frozen section, and in our case, we cannot just cut and immerse into alcohol. One, the fixative e.g. alcohol is not going to work for our antigen Two, we perform cryomicrotomy on a piece of tissue, collecting as many as a hundred sections, often serial and store the sections until staining (immunostaining in particular) can be performed. Air drying is a form of fixation, and the act of picking up a section onto a slide has been referred to as "flash drying". The antigens we need to see are better when air dried overnight, then fixed with either acetone or acetone/alcohol (in the case, for murine CD markers). Often air drying the section at RT and then fixing with acetone, and storing these fixed sections in a -80C freezer is very acceptable. If I were in a clinical setting and doing a routine H&E, I probably would do exactly as you do now. It is a matter of application. In our case, immersion immediately into a fixative is not optimal for our immunofluorescent or enzyme immunohistochemical results. If we want to see or identify where we are in a sample, we do exactly as you do, section and immerse into fixative, and then do a rapid H&E stain when we can or within a few minutes. We frequently immerse a frozen section into neutral buffered formalin and fix later in the day, week or whenever to have excellent morphology and staining results with an H&E. I hope this clarifies some parameters of performing cryotomy and staining versus how you do it. Gayle M. Callis HTL/HT/MTA(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Tuesday, August 24, 2010 5:07 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] preparation of frozen sections So as a pathologist, i have to ask you why you would want to air dry a section? From a diagnostic perspective, we consider air dried samples unacceptable in my lab. All of our standard histologic interpretation is based on fixed sections. So, why not drop the slide in a jar or ETOH and keep it there until you are ready to stain? Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com ------------------------------ Message: 7 Date: Tue, 24 Aug 2010 21:36:53 -0500 From: Jack Ratliff Subject: Re: [Histonet] Technovit 9100 New To: C B Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Cindy, I have very little experience with the Technovit kits as I predominantly use a MMA + DBP + Perkadox formulation for both thin and thick section histology. However, in my experience with the Technovit kits, I have discovered for me that the MMA + DBP + Perkadox type of formulation is more consistent, reproducible and overall more flexible for all types of microtomy applications and also for the variety of species (mouse to human) of bone tissue. I have more control over the quality of resin block I can produce with an MMA + DBP + Perkadox formulation, thin sections can be deplastified and staining is more routine and flexible. As for mounting thin microtomed sections, you need to coat your slides with some type of adhesive so that your sections will stay mounted throughout staining. I use Haupt's adhesive to coat my glass slides, along with an aluminum slide press and oven to activate the Haupt's media and complement the adhesion process. There is a step by step process that I can share with you if interested to help accomplish the section adhesion. Now with regards to cracking/crazing of ground (thick) sections in the presence of certain stains. I believe the best way to describe this is that these experiences are proportional to the density or hardness of the resin. If you have a hard resin section (based upon the overall hardness of you resin block), some chemicals will act to make the section brittle and crack similar to prolonged use of xylenes with undeminerized bone processing or prolonged paraffin infiltration is a soft tissue application. Jack On Aug 24, 2010, at 7:57 PM, C B wrote: > Anyone using Technovit 9100 for microtome and/or ground sections? Do you use > the routine Plus slides for mounting microtome sections or do the sections > require another type of adhesive? When staining the ground sections, do you > have any problems with certain solutions causing cracking/crazing? > Cindy Baranowski, HT (ASCP) > Saint Joseph's Translational Research Institute > Atlanta, Ga > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Tue, 24 Aug 2010 21:48:15 -0500 From: Jack Ratliff Subject: Re: [Histonet] Technovit 9100 New To: C B Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii One more thing I might add is that Dorn and Hart Microedge has just released a MMA + DBP + Perkadox kit (Acrylosin) in both a "hard" (ground/thick section formulation) and "soft" (thin section formulation). They are also in the process of carrying a lot of the stains that work well with this type of resin formulation as well as everything in between to assist in resin histology (i.e. slide presses, Haupt's, microtomy supplies/kits, etc). If you haven't already done so, just do an Internet search of their name and you should easily reach their website to view their current products. Please don't hesitate to contact me if you have any additional questions. Jack On Aug 24, 2010, at 7:57 PM, C B wrote: > Anyone using Technovit 9100 for microtome and/or ground sections? Do you use > the routine Plus slides for mounting microtome sections or do the sections > require another type of adhesive? When staining the ground sections, do you > have any problems with certain solutions causing cracking/crazing? > Cindy Baranowski, HT (ASCP) > Saint Joseph's Translational Research Institute > Atlanta, Ga > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Wed, 25 Aug 2010 08:51:18 +0200 From: louise renton Subject: Re: [Histonet] shrinkage To: "Edwards, Richard E." , Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 I seem to remember a good discussion in this in the following book:. L. P. Kok and M. E. Boon. *Microwave Cookbook for Microscopists*, Coulomb Press Leyden, Leiden (1992) p. 1432 . Whetehr or not it is still available is another matter On Tue, Aug 24, 2010 at 5:17 PM, Edwards, Richard E. wrote: > > Anybody aware of the degree of shrinkage in paraffin processed tissues > and/or GMA processed tissues?, many thanks. > > Cheers > Richard Edwards > > Leicester University. > > Leicester U.K. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 10 Date: Wed, 25 Aug 2010 06:12:36 -0400 From: "Dana Settembre" Subject: Re: [Histonet] PMS2 To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Diane, When I was doing PMS2 I was using BD's mouse antibody @ 1:10 Retreived with Dako's TRS in a steamer for 40 min, incubated the antibody for 60 minutes and I used a labelled polymer for the detection (Dako's Envision + Mouse) It was difficult to work up. Good Luck, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> 08/24/10 8:47 PM >>> Can anyone share their protocol for PMS2? I just keep getting background staining. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 25 Aug 2010 06:38:36 -0700 (PDT) From: C B Subject: [Histonet] porcine CD31 FFPE To: Histonet@lists.utsouthwestern.edu Message-ID: <459879.15305.qm@web114004.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Has anyone used the Serotec # MCA 1746G mouse anti-porcine CD31 or #MCA 1747 mouse anti-porcine CD31 on FFPE porcine arteries? If so, can you give recommendations for retrieval and dilutions? The website shows they have "Not determined" reactivity. Thanks in advance for any recommendations. Cindy Baranowski, HT(ASCP) ------------------------------ Message: 12 Date: Wed, 25 Aug 2010 06:42:50 -0700 (PDT) From: Chakib Boussahmain Subject: [Histonet] Lectin From Arachis hypogaea(peanut)- peroxidase Staining To: histonet@lists.utsouthwestern.edu Message-ID: <67839.6324.qm@web58105.mail.re3.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi Histonet, I am trying to do some staining using Lectin from Arachis hypogaea(peanut)-Peroxidase( FROM SIGMA), and wondering if anyone uses that stain if so, could you please share the protocol with me? Your help will be much appreciated! Thank you Chakib HTL From MIT ------------------------------ Message: 13 Date: Wed, 25 Aug 2010 09:45:48 -0400 From: "Houston, Ronald" Subject: RE: [Histonet] Ventana vs Leica To: "'Rathborne, Toni'" , Pamela Marcum , Maria Katleba Cc: histonet , "BSullivan@shorememorial.org" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Bond III hands down; I know Ventana are cutting pricing drastically to get machines in to labs, but the Bond is much more user friendly, especially if you use concentrated antibodies, and also employs the same detection kit for ISH as it does for IHC Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 24, 2010 5:10 PM To: Pamela Marcum; Maria Katleba Cc: histonet; BSullivan@shorememorial.org; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana vs Leica We recently acquired the Bond III. It was a tough decision, but space was the main reason we went with Leica. The Ventana sales team that visited our lab was very eager to get the account. Their pricing was not much different. They even offered to credit us for additional waste disposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pamela Marcum Sent: Tuesday, August 24, 2010 3:48 PM To: Maria Katleba Cc: histonet; BSullivan@shorememorial.org; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica I totally agree and we have an Ultra, 2 XTs and 3 Benchmarks. They are good systems that need to get cheaper and more flexible. I am demoing the Bond III and love the ease of use, flexibility and cost savings, most. Maria is right it is about 40% less to run than a Ventana. Pam Marcum AP Manager UAMS Little Rock, AR ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. Does IHC and ISH (yes- so does the Ventana) 2. Continuous feed (Ventana does NOT offer this!!!!) 3. Space saver, much smaller footprint than Benchmark 4. No wasted antibodies... 5. Not forced to buy EXPENSIVE prep kits either.... 6. Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. Won't blow tissue off the slide! 8. No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren To Sent by: histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. Does IHC and ISH (yes- so does the Ventana) 2. Continuous feed (Ventana does NOT offer this!!!!) 3. Space saver, much smaller footprint than Benchmark 4. No wasted antibodies... 5. Not forced to buy EXPENSIVE prep kits either.... 6. Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. Won't blow tissue off the slide! 8. No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren To Sent by: histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 14 Date: Wed, 25 Aug 2010 14:49:55 +0100 From: "Edwards, Richard E." Subject: [Histonet] shrinkage/a howlong is a piece of string type question To: "histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8D777B9E9@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Many thanks to all who responded, for paraffin processed tissues the figures suggested for the amount of shrinkage found or expected were :- "more than 5%":"5-10%":"10%"(twice):"10-15%":"20%":"25%":"30-35%":"30-40%", one responder felt it was "noticeable" and another thought it was a "fairy tale" concocted by pathologists............unsurprisingly many responders thought that the degree of shrinkage was dependent on the fixative used, processing schedule and the nature of the tissue itself, e.g. amount of lipid present. As far as shrinkage with GMA processed tissue go, a single response of "5%" was quoted. Richard Edwards ------------------------------ Message: 15 Date: Wed, 25 Aug 2010 08:57:19 -0700 From: Maria Katleba Subject: RE: [Histonet] Ventana vs Leica To: "Rathborne, Toni" , Pamela Marcum Cc: histonet , "BSullivan@shorememorial.org" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Toni brings up a very good point.... you know it's pretty bad when a company is willing to pay your waste costs... Not a good business plan. Why not 'fix' the machine so that it's more "green" Maria Katleba MS HT(ASCP) -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Tuesday, August 24, 2010 2:10 PM To: Pamela Marcum; Maria Katleba Cc: histonet; BSullivan@shorememorial.org; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana vs Leica We recently acquired the Bond III. It was a tough decision, but space was the main reason we went with Leica. The Ventana sales team that visited our lab was very eager to get the account. Their pricing was not much different. They even offered to credit us for additional waste disposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pamela Marcum Sent: Tuesday, August 24, 2010 3:48 PM To: Maria Katleba Cc: histonet; BSullivan@shorememorial.org; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica I totally agree and we have an Ultra, 2 XTs and 3 Benchmarks. They are good systems that need to get cheaper and more flexible. I am demoing the Bond III and love the ease of use, flexibility and cost savings, most. Maria is right it is about 40% less to run than a Ventana. Pam Marcum AP Manager UAMS Little Rock, AR ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. Does IHC and ISH (yes- so does the Ventana) 2. Continuous feed (Ventana does NOT offer this!!!!) 3. Space saver, much smaller footprint than Benchmark 4. No wasted antibodies... 5. Not forced to buy EXPENSIVE prep kits either.... 6. Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. Won't blow tissue off the slide! 8. No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren To Sent by: histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Maria Katleba" To: BSullivan@shorememorial.org, "Jay Lundgren" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Sent: Tuesday, August 24, 2010 2:32:08 PM Subject: RE: [Histonet] Ventana vs Leica Hi Jay, I have the Ventana Benchmark XT...love it......BUT Leica is LESS EXPENSIVE!!! Reasons to buy Leica Bond: 1. Does IHC and ISH (yes- so does the Ventana) 2. Continuous feed (Ventana does NOT offer this!!!!) 3. Space saver, much smaller footprint than Benchmark 4. No wasted antibodies... 5. Not forced to buy EXPENSIVE prep kits either.... 6. Cost to run with reagents is about 40% less than Ventana- Yes! I did my own cost analysis 7. Won't blow tissue off the slide! 8. No where near the waste that Benchmark has!!! I went to the Leica Symposium in San Francisco last week. I was able to ask many grueling questions... They did a very good job of honestly addressing each one. Honestly, if I was asked right now to buy...it would be Leica! Regards, Maria Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 12:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren To Sent by: histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 16 Date: Wed, 25 Aug 2010 09:59:11 -0600 From: "gayle callis" Subject: Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question To: "'Edwards, Richard E.'" , Message-ID: <000401cb446e$7768f690$663ae3b0$@callis@bresnan.net> Content-Type: text/plain; charset="us-ascii" Have you ever thought of doing a shrinkage test? Take a tissue specimen, and xerox or use a flat bed scanner. Put fixed sample between plastic sheets, and scan it as unfixed tissue, fixed before processing and then after processing while in a faced paraffin block. Take all the measurements and then do the calculations./ We used to xerox large stained bone sections, a clever way of getting a precise macro-images of a huge specimen to show gross features of a defect. This did a better job than trying to do a macro-photo with a camera or through a microscope (the latter doesn't happen). Years ago, when preparing for HTL exam practical, the samples e.g. tissue sections submitted had to be within a certain size range, and it was duly noted that after processing, the samples had shrinkage. This required going back to fixed tissue and cutting a bigger piece to compensate for the shrinkage and have a final correct sample/section size to follow the practical rules. As for GMA, there is a special processing schedule given to me that does not use alcohol dehydration (for lipid staining work). This protocol uses an GMA/watergradient since GMA is miscible with water. I would think there would be even less shrinkage with a water/GMA gradient and the source of shrinkage would come from the heat of polymerization and possibly a bit from kind of fixative used. The heat can controlled to some degree by doing polymerization on ice, or in a refrigerator, with the round JB4 metal chucks to dissipate the heat. Once again, I agree with Bryan Hewlett's assessment of shrinkage. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Wednesday, August 25, 2010 7:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shrinkage/a howlong is a piece of string type question Many thanks to all who responded, for paraffin processed tissues the figures suggested for the amount of shrinkage found or expected were :- "more than 5%":"5-10%":"10%"(twice):"10-15%":"20%":"25%":"30-35%":"30-40%", one responder felt it was "noticeable" and another thought it was a "fairy tale" concocted by pathologists............unsurprisingly many responders thought that the degree of shrinkage was dependent on the fixative used, processing schedule and the nature of the tissue itself, e.g. amount of lipid present. As far as shrinkage with GMA processed tissue go, a single response of "5%" was quoted. Richard Edwards _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __________ The message was checked by ESET Smart Security. http://www.eset.com ------------------------------ Message: 17 Date: Wed, 25 Aug 2010 11:26:38 -0500 From: Jan.Minshew@leica-microsystems.com Subject: Re: Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question To: "gayle callis" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hey lady, How are you? I haven't seen you on Histonet much lately. I hope that means that you are doing fun things and not working so hard. We have settled in Plano. It's so nice to be around family! Will I see you at NSH? If so, we have to have our night out again so we can catch up on gossip... Kind regards, Jan Minshew Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 Office: 847.405.7051 Cell: 847.970.8468 Fax: 847.405.6560 www.leica-microsystems.com Click Here for this month's special offers! [1]http://www.leica-microsystems.com/bsdspecial "gayle callis" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2010 10:59 AM To "'Edwards, Richard E.'" , cc Subject Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question Have you ever thought of doing a shrinkage test? Take a tissue specimen, and xerox or use a flat bed scanner. Put fixed sample between plastic sheets, and scan it as unfixed tissue, fixed before processing and then after processing while in a faced paraffin block. Take all the measurements and then do the calculations./ We used to xerox large stained bone sections, a clever way of getting a precise macro-images of a huge specimen to show gross features of a defect. This did a better job than trying to do a macro-photo with a camera or through a microscope (the latter doesn't happen). Years ago, when preparing for HTL exam practical, the samples e.g. tissue sections submitted had to be within a certain size range, and it was duly noted that after processing, the samples had shrinkage. This required going back to fixed tissue and cutting a bigger piece to compensate for the shrinkage and have a final correct sample/section size to follow the practical rules. As for GMA, there is a special processing schedule given to me that does not use alcohol dehydration (for lipid staining work). This protocol uses an GMA/watergradient since GMA is miscible with water. I would think there would be even less shrinkage with a water/GMA gradient and the source of shrinkage would come from the heat of polymerization and possibly a bit from kind of fixative used. The heat can controlled to some degree by doing polymerization on ice, or in a refrigerator, with the round JB4 metal chucks to dissipate the heat. Once again, I agree with Bryan Hewlett's assessment of shrinkage. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Wednesday, August 25, 2010 7:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shrinkage/a howlong is a piece of string type question Many thanks to all who responded, for paraffin processed tissues the figures suggested for the amount of shrinkage found or expected were :- "more than 5%":"5-10%":"10%"(twice):"10-15%":"20%":"25%":"30-35%":"30-40%", one responder felt it was "noticeable" and another thought it was a "fairy tale" concocted by pathologists............unsurprisingly many responders thought that the degree of shrinkage was dependent on the fixative used, processing schedule and the nature of the tissue itself, e.g. amount of lipid present. As far as shrinkage with GMA processed tissue go, a single response of "5%" was quoted. Richard Edwards _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5394 (20100824) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5396 (20100825) __________ The message was checked by ESET Smart Security. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ References 1. http://www.leica-microsystems.com/bsdspecial ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 33 **************************************** Email Confidentiality Notice: The information contained in this transmission is confidential, proprietary or privileged and may be subject to protection under the law, including the Health Insurance Portability and Accountability Act (HIPAA). The message is intended for the sole use of the individual or entity to whom it is addressed. If you are not the intended recipient, you are notified that any use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalties. If you received this transmission in error, please contact the sender immediately by replying to this email and delete the material from any computer. From Janice.Mahoney <@t> alegent.org Thu Aug 26 09:08:19 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Thu Aug 26 09:11:59 2010 Subject: [Histonet] Ventana vs Leica In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A448@EXCHMBC2.ad.ah.local> We love our Ventana instruments too Jay. I don't quite believe the 40% difference in cost. I'd like to see those numbers. I know I save in tech time and the instruments are very easy to use. We have histo assistants and secretaries trained to load and unload the instruments, saving out techs to do the things only techs can do. Talk about LEAN! A little more from a LEAN perspective, the Ultra is the only instrument out there that is "TRUE" continuous flow. As soon as there is an open spot on the instrument and the antibody is on board, I can add a slide. I don't have to wait till one of the 10 slide modules is finished. Leica is still a batch instrument, it is just smaller batches than the older IHC models. I'm not putting Leica down, it is a fine instrument but I think it is important for people to know the facts. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 2:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren To Sent by: histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From MLashus <@t> pathgroup.com Thu Aug 26 09:35:27 2010 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Thu Aug 26 09:35:33 2010 Subject: [Histonet] Ventana vs Leica In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A448@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A448@EXCHMBC2.ad.ah.local> Message-ID: <197CD0B02A81F94994A285C59C8AE05C05D6F490BF@pgnexchange.pathgroup.com> I agree with you Jan; we love our Ventana Instruments (3 XTs, 1 Ultra). I would trade my XTs for 3 more Ultras if I could talk Ventana into it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Thursday, August 26, 2010 10:08 AM To: 'BSullivan@shorememorial.org'; Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana vs Leica We love our Ventana instruments too Jay. I don't quite believe the 40% difference in cost. I'd like to see those numbers. I know I save in tech time and the instruments are very easy to use. We have histo assistants and secretaries trained to load and unload the instruments, saving out techs to do the things only techs can do. Talk about LEAN! A little more from a LEAN perspective, the Ultra is the only instrument out there that is "TRUE" continuous flow. As soon as there is an open spot on the instrument and the antibody is on board, I can add a slide. I don't have to wait till one of the 10 slide modules is finished. Leica is still a batch instrument, it is just smaller batches than the older IHC models. I'm not putting Leica down, it is a fine instrument but I think it is important for people to know the facts. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Tuesday, August 24, 2010 2:05 PM To: Jay Lundgren Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren To Sent by: histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From Dorothy.L.Webb <@t> HealthPartners.Com Thu Aug 26 10:53:58 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Aug 26 10:54:05 2010 Subject: [Histonet] Cleaning molds Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!!!!!! Thanks all! Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From MSHERWOOD <@t> PARTNERS.ORG Thu Aug 26 11:14:44 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Aug 26 11:15:00 2010 Subject: [Histonet] Cleaning molds In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E245CC@PHSXMB30.partners.org> We clean our stainless steel base molds by soaking in solvent (i.e. citrisolv, etc.) then wash, by hand, with soap and water. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, August 26, 2010 11:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cleaning molds Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!!!!!! Thanks all! Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From chapcl <@t> yahoo.com Thu Aug 26 11:16:34 2010 From: chapcl <@t> yahoo.com (chapcl@yahoo.com) Date: Thu Aug 26 11:16:46 2010 Subject: [Histonet] Cleaning molds In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> Message-ID: <791677523-1282839395-cardhu_decombobulator_blackberry.rim.net-1965674517-@bda104.bisx.prod.on.blackberry> Back when I was using metal cassette lids and an autotechnicon (so no cleaning in the processor) I would throw all the lids and molds into a pot after embedding. Throw a bit of laboratory detergent in, fill with water, and boil on a hot plate. Once I got to a rolling boil, I would turn the heat off. By the end of the day it was cool. Peel the paraffin off the top, then rinse the molds. Worked great! Didn't even need mold release. Will Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Webb, Dorothy L" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Thu, 26 Aug 2010 10:53:58 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cleaning molds Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!!!!!! Thanks all! Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Aug 26 11:30:47 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 26 11:30:54 2010 Subject: [Histonet] Cleaning molds In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> Message-ID: <856003.38414.qm@web65715.mail.ac4.yahoo.com> The best way to clean the molds is to boil them in a 2% aq. sol. of dishwasher detergent. Now, this is an extra step so if you clean the tissue processor anyway, it is one chose less to just place them in the cleaning cycle.Ren? J. --- On Thu, 8/26/10, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Cleaning molds To: "'histonet@lists.utsouthwestern.edu'" Date: Thursday, August 26, 2010, 11:53 AM Does anyone in histoland clean their embedding molds in a dishwasher?? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds??? Simple, but plaguing question!!!!!!!? Thanks all! Dorothy Webb ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Aug 26 11:52:43 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Aug 26 11:52:51 2010 Subject: AW: [Histonet] Cleaning molds In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> Message-ID: <59E4B34C09F74720A3CE2AEF6A572C4E@dielangs.at> We use an ultrasonic bath filled with water with a bit of dishwasher. Temperature about 40 allows the paraffin to get off the molds and to swim on the surface. Needs 15 min. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Webb, Dorothy L Gesendet: Donnerstag, 26. August 2010 17:54 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Cleaning molds Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!!!!!! Thanks all! Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barbara.Crill <@t> LPNT.net Thu Aug 26 12:16:14 2010 From: Barbara.Crill <@t> LPNT.net (Barbara.Crill@LPNT.net) Date: Thu Aug 26 12:16:22 2010 Subject: [Histonet] ventana & HPv Message-ID: <7DA79EBDBD92BF408EF392413737878D3916A2E0D8@NADCWPMSGCMS01.hca.corpad.net> Does anyone use the Ventana to process HPV for tissue and paps? Barbara Crill DRMC From gfraga <@t> kumc.edu Thu Aug 26 12:21:05 2010 From: gfraga <@t> kumc.edu (Garth Fraga) Date: Thu Aug 26 12:21:31 2010 Subject: [Histonet] Rapid liver core biopsy processing Message-ID: <4C765C310200001B00064A21@smtpout.kumc.edu> Dear histonetters, We do about a hundred liver transplants/year at our hospital, and the hepatologists do lots of liver core biopsies to rule out rejection. They want a same-day diagnosis on these, so historically they have been rush processed on a two-hour cycle (VIP machine). They are brought over directly from the liver biopsy suite immediately after biopsy, so they get very little fixation in the specimen container prior to going on the processor. Recently we had a couple of sub-par cases and have moved to a four-hour processing cycle. Do any of you have any experience dealing with rush-processed liver cores in transplant patients? What sort of a processing schedule do you recommend? Anyone handling them like frozen sections? Thanks, Garth Fraga (pathologist) University of Kansas Medical Center From PMonfils <@t> Lifespan.org Thu Aug 26 14:00:45 2010 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Aug 26 14:00:50 2010 Subject: [Histonet] Microtome Repair - New England Area? Message-ID: <4EBFF65383B74D49995298C4976D1D5E07174CE0@LSRIEXCH1.lsmaster.lifespan.org> Any recommendations for microtome repairs (preferably on-site), preferably in the MA-RI-CT area? Thanks. From cgill <@t> marylandgeneral.org Thu Aug 26 14:37:41 2010 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Thu Aug 26 14:37:48 2010 Subject: [Histonet] Rapid liver core biopsy processing In-Reply-To: <4C765C310200001B00064A21@smtpout.kumc.edu> References: <4C765C310200001B00064A21@smtpout.kumc.edu> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9ED8@MDGEN-EXCH1.marylandgeneral.org> We handle them when we get them as frozen sections. The Pathologist are able to give a rapid diagnosis but they do prefer the formalin fixed tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garth Fraga Sent: Thursday, August 26, 2010 1:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rapid liver core biopsy processing Dear histonetters, We do about a hundred liver transplants/year at our hospital, and the hepatologists do lots of liver core biopsies to rule out rejection. They want a same-day diagnosis on these, so historically they have been rush processed on a two-hour cycle (VIP machine). They are brought over directly from the liver biopsy suite immediately after biopsy, so they get very little fixation in the specimen container prior to going on the processor. Recently we had a couple of sub-par cases and have moved to a four-hour processing cycle. Do any of you have any experience dealing with rush-processed liver cores in transplant patients? What sort of a processing schedule do you recommend? Anyone handling them like frozen sections? Thanks, Garth Fraga (pathologist) University of Kansas Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrick.lewis <@t> seattlechildrens.org Thu Aug 26 15:32:42 2010 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Thu Aug 26 15:32:51 2010 Subject: [Histonet] Sanosil is it the right answer to all my disinfecting problems Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C05814CCF@s107.childrens.sea.kids> Has anyone used sanosil as a disinfecting agent? Could it be used to disinfect a cryostat? Is it possible to use its fogging method to make sure that all the crevices within a cryostat are covered with disinfectant. Would sanosil affect future slides that are made in the cryostat that has residual sanosil on its surfaces? Inquiring minds want to know. Patrick. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Rcartun <@t> harthosp.org Thu Aug 26 15:54:25 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Aug 26 15:54:34 2010 Subject: [Histonet] Lung biopsies - Question Message-ID: <4C769C41.7400.0077.1@harthosp.org> How many labs cut extra tissue sections (or tissue curls) for molecular testing (KRAS, BRAF, EGFR, etc.) on lung biopsies up front (at the time of H&E sectioning)? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From dchihc <@t> yahoo.com Thu Aug 26 15:59:51 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Thu Aug 26 15:59:54 2010 Subject: [Histonet] Histology Position In Alabama Message-ID: <935758.14226.qm@web43516.mail.sp1.yahoo.com> FINALLY another HistoTech position has been approved at DCH Regional Medical Center in Tuscaloosa, Alabama. We are a not for profit facility in West Alabama about 50 miles from Birmingham,?about 4 hours from the Gulf of Mexico, and about 3 hours from Atlanta. We process about 15000 surgicals per year using Sakura VIP conventional processor, and Sakura?ExPress 50 Rapid Tissue Processor, Sakura Prisma H&E Stainer with tape coverslipper and Ventana IHC automation. Hopefully we will be automated in Special Stains after October 1. Interested candidates must be proficient in embedding, microtomy, frozen sections, and (for now) manual special staining. Please contact Michelle Fagin at 205-759-7762 or Fax?205-750-5224 OR ????????????????????? Sherrie Faulkner at 205-750-5736 or email mfagin@dchsystem.com ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From billodonnell <@t> catholichealth.net Thu Aug 26 16:28:20 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Aug 26 16:28:29 2010 Subject: [Histonet] Sanosil is it the right answer to all my disinfectingproblems In-Reply-To: <7EA5752B2903B143A5B845DEA87D5D1C05814CCF@s107.childrens.sea.kids> References: <7EA5752B2903B143A5B845DEA87D5D1C05814CCF@s107.childrens.sea.kids> Message-ID: Patrick, We use it in our new cryostat. It is the recommended disinfectant for the auto-disifecting feature. It uses a (cold-fog) process, unlike some that actually heat up the solution. I am unsure if heated Sanosil is effective or not. I have not noticed any resiual. If it does leave residual, I would think that some 100% ROH would remove it. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Thursday, August 26, 2010 3:33 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Sanosil is it the right answer to all my disinfectingproblems Has anyone used sanosil as a disinfecting agent? Could it be used to disinfect a cryostat? Is it possible to use its fogging method to make sure that all the crevices within a cryostat are covered with disinfectant. Would sanosil affect future slides that are made in the cryostat that has residual sanosil on its surfaces? Inquiring minds want to know. Patrick. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raestask <@t> grics.net Thu Aug 26 17:19:28 2010 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Thu Aug 26 17:20:00 2010 Subject: [Histonet] Lung biopsies - Question In-Reply-To: <4C769C41.7400.0077.1@harthosp.org> References: <4C769C41.7400.0077.1@harthosp.org> Message-ID: <5458BB60457D418AB17283A3AAC6EB5A@your4105e587b6> We cut 10 unstained slides. Rae Staskiewicz Methodist Medical Center of Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, August 26, 2010 3:54 PM To: Histonet Subject: [Histonet] Lung biopsies - Question How many labs cut extra tissue sections (or tissue curls) for molecular testing (KRAS, BRAF, EGFR, etc.) on lung biopsies up front (at the time of H&E sectioning)? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Thu Aug 26 19:15:17 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Aug 26 19:16:26 2010 Subject: [Histonet] Lung biopsies - Question In-Reply-To: <5458BB60457D418AB17283A3AAC6EB5A@your4105e587b6> References: <4C769C41.7400.0077.1@harthosp.org> <5458BB60457D418AB17283A3AAC6EB5A@your4105e587b6> Message-ID: We cut 10 unstained slides as well. Michelle On Aug 26, 2010, at 6:19 PM, "Rae Staskiewicz" wrote: > We cut 10 unstained slides. > > Rae Staskiewicz > Methodist Medical Center of Illinois > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard > Cartun > Sent: Thursday, August 26, 2010 3:54 PM > To: Histonet > Subject: [Histonet] Lung biopsies - Question > > How many labs cut extra tissue sections (or tissue curls) for molecular > testing (KRAS, BRAF, EGFR, etc.) on lung biopsies up front (at the time of > H&E sectioning)? Thanks. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histotech <@t> imagesbyhopper.com Thu Aug 26 19:21:08 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Aug 26 19:22:12 2010 Subject: [Histonet] Ventana vs Leica In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A448@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A448@EXCHMBC2.ad.ah.local> Message-ID: Unless someone corrects me (or even agrees with me!) in FL only a technologist is allowed to load the IHC machine, so no additional lean for us. :o( I would be interested to hear more about the savings though, as we are preparing to be in the market for a new IHC machine. We currently have the Benchmark XT. On Aug 26, 2010, at 10:08 AM, "Mahoney,Janice A" wrote: > We love our Ventana instruments too Jay. I don't quite believe the 40% difference in cost. I'd like to see those numbers. I know I save in tech time and the instruments are very easy to use. We have histo assistants and secretaries trained to load and unload the instruments, saving out techs to do the things only techs can do. Talk about LEAN! > A little more from a LEAN perspective, the Ultra is the only instrument out there that is "TRUE" continuous flow. As soon as there is an open spot on the instrument and the antibody is on board, I can add a slide. I don't have to wait till one of the 10 slide modules is finished. Leica is still a batch instrument, it is just smaller batches than the older IHC models. I'm not putting Leica down, it is a fine instrument but I think it is important for people to know the facts. > Jan Mahoney > Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org > Sent: Tuesday, August 24, 2010 2:05 PM > To: Jay Lundgren > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Ventana vs Leica > > Jay, > I currently use the Ventana and am very pleased with the results I get. > The only draw back is the cost to run the instrument. It can get quite > pricey. They added space on the antibody wheel but took space away from the > slide area. This has impacted our work flow greatly. We are however looking > to purchase a second one. This one will have continual through put. That > should help out with TAT. Hope this helps. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > > Jay Lundgren > l.com> To > Sent by: histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] Ventana vs Leica > 08/24/2010 02:30 > PM > > > > > > > > > I was wondering if anyone out there had experience with both the > Ventana Ultra and the Leica Bond immunostainers. I realize that most > people > have a personal preference as to brands, but I'm not looking for a > knee-jerk > opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had > actual > experience working on a daily basis with both instruments. If this is you, > could you please tell me which you preferred and why. > I'm currently working for a facility in MT which has narrowed down its > search to these two instruments. No vendors please, they've already given > their pitches. > > Thanks, > Jay A. > Lundgren M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DianaRip1 <@t> aol.com Thu Aug 26 19:33:02 2010 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Thu Aug 26 19:33:20 2010 Subject: [Histonet] Cleaning molds Message-ID: <693eb.5e258271.39a861be@aol.com> We throw ours in a pot with a little soap in the bottom and boil, unplug and let the paraffin harden. Just take the paraffin disk off and rinse the molds in cold water and lay out to dry. No reason to gunk up the expensive tissue processor. From DianaRip1 <@t> aol.com Thu Aug 26 19:33:58 2010 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Thu Aug 26 19:34:10 2010 Subject: [Histonet] ventana & HPv Message-ID: <69490.415f4af4.39a861f6@aol.com> We used to do pap HPV's on our Ventana. Whatcha need? From Jason.PALMER <@t> svhm.org.au Thu Aug 26 22:31:39 2010 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Thu Aug 26 22:31:45 2010 Subject: [Histonet] cytokeratin 18 in liver Message-ID: Hello all. Just wondering if anyone has experience with cytokeratin 18 immunohistochemistry labelling in liver (FFPE)? I have used an Abcam rabbit anti human CK18 to try and label hepatocytes in both human and mouse adult liver and have found that I get strong labelling in bile duct epithelia but essentially nothing in hepatocytes. I say essentially nothing, but there does appear to be faint possible specific staining in a few hepatocytes. From my reading, I had thought that cytokeratin 18 (and 8) are abundantly expressed in hepatocytes and should be easy to label, but that is not what I have found. We are trying to tissue engineer liver from progenitor cells in a mouse model, and need to be able to identify these cells, whether liver progenitors or mature / differentiated hepatocytes, hence my choice of an antibody against CK18 which should label both. We have also used a pan cytokeratin (Dako Z0622) with mixed results and were hoping for something a little more defined. Any thoughts greatly appreciated, Jason Jason Palmer Histology Laboratory Coordinator O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Susan.Walzer <@t> HCAHealthcare.com Fri Aug 27 02:16:30 2010 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Fri Aug 27 02:16:37 2010 Subject: [Histonet] RE: Cleaning molds In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2B32B0EBB5@FWDCWPMSGCMS09.hca.corpad.net> Ours go on the VIP during the cleaning cycle. Then we dip them in a bucket of alcohol containing mold release and spread them out to dry on a clean towel. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, August 26, 2010 11:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cleaning molds Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!!!!!! Thanks all! Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri Aug 27 05:02:13 2010 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Aug 27 05:02:12 2010 Subject: [Histonet] Lung biopsies - Question In-Reply-To: <4C769C41.7400.0077.1@harthosp.org> Message-ID: We cut 6 extra up front. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, August 26, 2010 4:54 PM To: Histonet Subject: [Histonet] Lung biopsies - Question How many labs cut extra tissue sections (or tissue curls) for molecular testing (KRAS, BRAF, EGFR, etc.) on lung biopsies up front (at the time of H&E sectioning)? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Barry.R.Rittman <@t> uth.tmc.edu Fri Aug 27 07:33:31 2010 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Aug 27 07:37:05 2010 Subject: [Histonet] RE: Cleaning molds In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2B32B0EBB5@FWDCWPMSGCMS09.hca.corpad.net> References: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int>, <4BF03F5404EBDE409AF9232DA74B9DED2B32B0EBB5@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E904A24F82@UTHCMS1.uthouston.edu> Dorothy trying to be "green". Whatever solution you use it is probably best if you can leave the steel molds in an oven for s short time to remove excess wax before the final step. This will decrease the amount of solvent or soap you need to use. Or you can just heat in soapy water, allow to cool, remove wax on surface of fluid and then quick rinse. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com [Susan.Walzer@HCAHealthcare.com] Sent: Friday, August 27, 2010 2:16 AM To: Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cleaning molds Ours go on the VIP during the cleaning cycle. Then we dip them in a bucket of alcohol containing mold release and spread them out to dry on a clean towel. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, August 26, 2010 11:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cleaning molds Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!!!!!! Thanks all! Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Fri Aug 27 08:50:01 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Aug 27 08:50:17 2010 Subject: [Histonet] Cutting standards In-Reply-To: <12A4DAFC2FEBB84B8DED5F5E9201B4E904A24F82@UTHCMS1.uthouston.edu> Message-ID: <53FAA169A6FD42D8B5F1A6D18EA7E417@hopperPC> I know this question has been asked before ... Can anyone share with me what they are actually using as a cutting/embedding standard for your techs? For instance, how many seconds (mins?) do you allow for embedding a block? How many seconds(mins?) do you allow for cutting a block? For simplicity here, I am looking at the "plop and drop" type specimens, ie larger specimens that don't require specific orientation and can be placed in a mold easily. These types of blocks will generally have one section on one slide. I am trying to find out if the standard I have for my techs is too tough or too lenient on them. I allow 45 seconds to embed such a block and another 45 seconds to section that same block. How does that fit with what you guys are all doing? Thanks! Michelle From jnocito <@t> satx.rr.com Fri Aug 27 08:53:00 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Aug 27 08:53:28 2010 Subject: [Histonet] Cleaning molds In-Reply-To: <791677523-1282839395-cardhu_decombobulator_blackberry.rim.net-1965674517-@bda104.bisx.prod.on.blackberry> References: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> <791677523-1282839395-cardhu_decombobulator_blackberry.rim.net-1965674517-@bda104.bisx.prod.on.blackberry> Message-ID: yeah, I'd be careful of this method. We used to do this at the U.S. Air Force hospital I used to work at when I was active duty. One time, the fire alarm went off and we had to evacuate the lab. Well, there must have been a real problem because we were out of the lab a long time. By the time we got the "all clear" to go back into the lab, I was the first one back and ran into some firemen. Seems the water boiled out of the pot and the paraffin was smoking up a storm.. No one said anything, but I bet that was why we were out of the lab so long. Now, those of you that have worked in a military or government hospital realize all the freaking paperwork that had to be filled out from that one incident. We were ordered by Safety to develop another method. So, we soak our molds in xylene for a couple of hours, covered of course as not expose the lab to the xylene fumes (that would required addition freaking paperwork) followed by a 100% etoh rinse, followed by air drying. JTT ----- Original Message ----- From: To: "Webb, Dorothy L" ; ; Sent: Thursday, August 26, 2010 11:16 AM Subject: Re: [Histonet] Cleaning molds > Back when I was using metal cassette lids and an autotechnicon (so no > cleaning in the processor) I would throw all the lids and molds into a pot > after embedding. Throw a bit of laboratory detergent in, fill with water, > and boil on a hot plate. Once I got to a rolling boil, I would turn the > heat off. By the end of the day it was cool. Peel the paraffin off the > top, then rinse the molds. > > Worked great! Didn't even need mold release. > > Will > > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: "Webb, Dorothy L" > Sender: histonet-bounces@lists.utsouthwestern.edu > Date: Thu, 26 Aug 2010 10:53:58 > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Cleaning molds > > Does anyone in histoland clean their embedding molds in a dishwasher? > Otherwise, besides placing in the cleaning cycle of your processor, how do > sites clean their molds?? Simple, but plaguing question!!!!!!! Thanks > all! > > Dorothy Webb > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sgoebel <@t> xbiotech.com Fri Aug 27 09:10:38 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Aug 27 09:10:44 2010 Subject: [Histonet] Cutting standards Message-ID: <20100827071038.9e2d9aa830e8449a2412eb1e4f2f067e.bc80d4cf57.wbe@email04.secureserver.net> Wow, you time your techs!?! Hard core!!! 45 seconds t sounds ok, but 45 seconds to section? What if the tissue need little extra care? Some are harder and some are fatty. If I had 45 seconds to cut a giant hunk of breast that would be alot of pr essure!!! Just remember crap in crap out...sometimes you have to take extra time t =) < Sarah Goebel, B.A., HT Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Tex (512)386-5107 -------- Original Message -------- Subject: [Histonet] Cutting standards From: <[1]histotech@imagesb Date: Fri, August 27, 2010 6:50 am To: <[2]histonet@lists I know this question has been asked before ... Can anyone share with me wha they are actually using as a cutting/embedding standard for your techs? Fo instance, how many seconds (mins?) do you allow for embedding a block? How many seconds(mins?) do you allow for cutting a block? For simplicity here, I am looking at the "plop and drop" type specimens, ie larger specimens that don't require specific orientation and can be placed< have one section on one slide. I am trying to find out if the standard I have for my techs is< such a block and another 45 seconds to section that same block. How does that fit with what you guys are all doing? Thanks! Michelle _______________________________________________ Histonet mailing list [3]Histonet@lists.utsouth [4]http: References 1. 3D"mailto:histotech@imagesbyhopper.com" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From NSEARCY <@t> swmail.sw.org Fri Aug 27 09:30:18 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Aug 27 09:30:27 2010 Subject: [Histonet] FITC on Ventana Ultra Message-ID: <4C7785AB.5D38.00EF.0@swmail.sw.org> Any users out there that have had A CAP inspector question negative control on the instrument? In regard to CAP question ANP.21850 in which the notes state, "A negative reagent control in which the patient tissue is processed in an identical manner to the test specimen but with the primary antibody omitted must be performed for each patient test specimen?" Below is the response from Ventana regarding "negatives" I am curios if CAP accepts these methods?? In regards to the question below about running a negative control fitc, there are two ways to accomplish this. 1) There is not currently a place in the protocol to select a fitc negative. We can still be compliant by ensuring the negative slide is treated the same as the patient. The only reagent the patient slide is exposed to, besides the fitc antibody, is reaction buffer. Running a negative can be accomplished by applying reaction buffer to the slide and letting it incubate for the same amount of time. Next, it is important to ensure both slides are coverslipped in the came mounting media. 2) The second method is to utilize internal negatives that are already present within the patient tissue. However, this is difficult when running Fitc antibodies such as IgG, IgM, etc. We are looking into adding this addition into the software. Many customers, however have expressed that they would rather utilize the slide drawer for another patient slide, rather than running the fitc negative. Am interested in your comments. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From lblazek <@t> digestivespecialists.com Fri Aug 27 09:30:37 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Aug 27 09:33:56 2010 Subject: [Histonet] Cutting standards In-Reply-To: <20100827071038.9e2d9aa830e8449a2412eb1e4f2f067e.bc80d4cf57.wbe@email04.secureserver.net> References: <20100827071038.9e2d9aa830e8449a2412eb1e4f2f067e.bc80d4cf57.wbe@email04.secureserver.net> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EAF72C789@IBMB7Exchange.digestivespecialists.com> I'm up for the Margaritas! I agree with the 45 seconds at cutting a block is not a good time measure. I think that if you are including cleaning the edges of the block, facing the block, making a slide for the block etc. then 2 minutes is a good average. I think years and years ago that was what one of the time studies I participated in figured was an average time. However, I don't think timing someone that cutting is a very good practice with all of the variables involved. I think rather than timing someone I would rather, if a tech seems to be very slow, observing why that particular person is significantly slower than others and see if there is a way to increase their productivity. My two cents worth! Now you have four cents! Now! Anyone looking for a job in the Dayton, Oh area? I have an opening for a tech. We have a great team and state of the art equipment. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Friday, August 27, 2010 10:11 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cutting standards Wow, you time your techs!?! Hard core!!! 45 seconds t=mbed sounds ok, but 45 seconds to section? What if the tissue need= little extra care? Some are harder and some are fatty. If I=ly had 45 seconds to cut a giant hunk of breast that would be alot of pr essure!!! Just remember crap in crap out...sometimes you have to take=ittle longer to do it right with one try instead of taking extra time t=o something you rushed again. Just my two cents? =) <=v>Happy Friday everyone...margaritas after work!!! Sarah Goebel, B.A., HT=SCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Tex= 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] Cutting standards From: <[1]histotech@imagesb=opper.com> Date: Fri, August 27, 2010 6:50 am To: <[2]histonet@lists=tsouthwestern.edu> I know this question has been asked before ... Can anyone share with me wha= they are actually using as a cutting/embedding standard for your techs? Fo= instance, how many seconds (mins?) do you allow for embedding a block? How many seconds(mins?) do you allow for cutting a block? For simplicity here, I am looking at the "plop and drop" type specimens, ie larger specimens that don't require specific orientation and can be placed<=> in a mold easily. These types of blocks will generally have one section on one slide. I am trying to find out if the standard I have for my techs is<=> too tough or too lenient on them. I allow 45 seconds to embed such a block and another 45 seconds to section that same block. How does that fit with what you guys are all doing? Thanks! Michelle _______________________________________________ Histonet mailing list [3]Histonet@lists.utsouth=stern.edu [4]http:=lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:histotech@imagesbyhopper.com" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Fri Aug 27 10:10:32 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Aug 27 10:10:54 2010 Subject: [Histonet] Cutting standards In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EAF72C789@IBMB7Exchange.digestivespecialists.com> Message-ID: :o) Okay, to clear something up, I am using the times as a AVERAGE for cutting and embedding over the course of the day. I do understand that there are easy blocks and difficult ones and the average between them! If I am to tell a tech they could work faster/more efficiently etc, I would feel better if I have some sort of a standard goal which they can aim for, hence the reason for my question. And no, I'm not being hard core! ;o) CAP even asks if there are standards set for your techs. I'm enjoying the discussion and thank you all for taking the time to help. :o) Michelle -----Original Message----- From: Blazek, Linda [mailto:lblazek@digestivespecialists.com] Sent: Friday, August 27, 2010 10:31 AM To: 'sgoebel@xbiotech.com'; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cutting standards I'm up for the Margaritas! I agree with the 45 seconds at cutting a block is not a good time measure. I think that if you are including cleaning the edges of the block, facing the block, making a slide for the block etc. then 2 minutes is a good average. I think years and years ago that was what one of the time studies I participated in figured was an average time. However, I don't think timing someone that cutting is a very good practice with all of the variables involved. I think rather than timing someone I would rather, if a tech seems to be very slow, observing why that particular person is significantly slower than others and see if there is a way to increase their productivity. My two cents worth! Now you have four cents! Now! Anyone looking for a job in the Dayton, Oh area? I have an opening for a tech. We have a great team and state of the art equipment. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Friday, August 27, 2010 10:11 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cutting standards Wow, you time your techs!?! Hard core!!! 45 seconds t=mbed sounds ok, but 45 seconds to section? What if the tissue need= little extra care? Some are harder and some are fatty. If I=ly had 45 seconds to cut a giant hunk of breast that would be alot of pr essure!!! Just remember crap in crap out...sometimes you have to take=ittle longer to do it right with one try instead of taking extra time t=o something you rushed again. Just my two cents? =) <=v>Happy Friday everyone...margaritas after work!!! Sarah Goebel, B.A., HT=SCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Tex= 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] Cutting standards From: <[1]histotech@imagesb=opper.com> Date: Fri, August 27, 2010 6:50 am To: <[2]histonet@lists=tsouthwestern.edu> I know this question has been asked before ... Can anyone share with me wha= they are actually using as a cutting/embedding standard for your techs? Fo= instance, how many seconds (mins?) do you allow for embedding a block? How many seconds(mins?) do you allow for cutting a block? For simplicity here, I am looking at the "plop and drop" type specimens, ie larger specimens that don't require specific orientation and can be placed<=> in a mold easily. These types of blocks will generally have one section on one slide. I am trying to find out if the standard I have for my techs is<=> too tough or too lenient on them. I allow 45 seconds to embed such a block and another 45 seconds to section that same block. How does that fit with what you guys are all doing? Thanks! Michelle _______________________________________________ Histonet mailing list [3]Histonet@lists.utsouth=stern.edu [4]http:=lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:histotech@imagesbyhopper.com" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3097 - Release Date: 08/27/10 06:34:00 From Bill.Tench <@t> pph.org Fri Aug 27 10:14:23 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Fri Aug 27 10:14:30 2010 Subject: [Histonet] cutting standards Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5567@MAIL1.pph.local> I asked this question earlier in the spring. someone sent me some national standards from surveys, so if you go to the archives you should find this information. i will see if i can find the information. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From rjbuesa <@t> yahoo.com Fri Aug 27 10:28:00 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 27 10:28:07 2010 Subject: [Histonet] Cutting standards In-Reply-To: <53FAA169A6FD42D8B5F1A6D18EA7E417@hopperPC> Message-ID: <154754.25584.qm@web65712.mail.ac4.yahoo.com> The averages from a survey of 325 histolabs is 50 blocks/hour to embed and 24 blocks/hour to cut. If interested I can send you an article about this. Ren? J. --- On Fri, 8/27/10, histotech@imagesbyhopper.com wrote: From: histotech@imagesbyhopper.com Subject: [Histonet] Cutting standards To: histonet@lists.utsouthwestern.edu Date: Friday, August 27, 2010, 9:50 AM I know this question has been asked before ... Can anyone share with me what they are actually using as a cutting/embedding standard for your techs?? For instance, how many seconds (mins?) do you allow for embedding a block?? How many seconds(mins?) do you allow for cutting a block? For simplicity here, I am looking at the "plop and drop" type specimens, ie larger specimens that don't require specific orientation and can be placed in a mold easily.? These types of blocks will generally have one section on one slide.? I am trying to find out if the standard I have for my techs is too tough or too lenient on them.? I allow 45 seconds to embed such a block and another 45 seconds to section that same block. How does that fit with what you guys are all doing? Thanks! Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Aug 27 10:32:53 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Aug 27 10:32:58 2010 Subject: [Histonet] Cleaning molds In-Reply-To: References: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> <791677523-1282839395-cardhu_decombobulator_blackberry.rim.net-1965674517-@bda104.bisx.prod.on.blackberry> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403321606D9@CHEXCMS10.one.ads.che.org> If you keep your molds in the hot tray of the embedding center, you never have to clean them... I promise. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, August 27, 2010 09:53 To: chapcl@yahoo.com; Webb, Dorothy L; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cleaning molds yeah, I'd be careful of this method. We used to do this at the U.S. Air Force hospital I used to work at when I was active duty. One time, the fire alarm went off and we had to evacuate the lab. Well, there must have been a real problem because we were out of the lab a long time. By the time we got the "all clear" to go back into the lab, I was the first one back and ran into some firemen. Seems the water boiled out of the pot and the paraffin was smoking up a storm.. No one said anything, but I bet that was why we were out of the lab so long. Now, those of you that have worked in a military or government hospital realize all the freaking paperwork that had to be filled out from that one incident. We were ordered by Safety to develop another method. So, we soak our molds in xylene for a couple of hours, covered of course as not expose the lab to the xylene fumes (that would required addition freaking paperwork) followed by a 100% etoh rinse, followed by air drying. JTT ----- Original Message ----- From: To: "Webb, Dorothy L" ; ; Sent: Thursday, August 26, 2010 11:16 AM Subject: Re: [Histonet] Cleaning molds > Back when I was using metal cassette lids and an autotechnicon (so no > cleaning in the processor) I would throw all the lids and molds into a > pot after embedding. Throw a bit of laboratory detergent in, fill with > water, and boil on a hot plate. Once I got to a rolling boil, I would > turn the heat off. By the end of the day it was cool. Peel the > paraffin off the top, then rinse the molds. > > Worked great! Didn't even need mold release. > > Will > > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: "Webb, Dorothy L" > Sender: histonet-bounces@lists.utsouthwestern.edu > Date: Thu, 26 Aug 2010 10:53:58 > To: > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Cleaning molds > > Does anyone in histoland clean their embedding molds in a dishwasher? > Otherwise, besides placing in the cleaning cycle of your processor, > how do sites clean their molds?? Simple, but plaguing question!!!!!!! > Thanks all! > > Dorothy Webb > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, > please be advised that you have received this e-mail in error and that > any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jbaez <@t> interscopepath.com Fri Aug 27 10:29:50 2010 From: jbaez <@t> interscopepath.com (Baez, Janet) Date: Fri Aug 27 10:33:28 2010 Subject: [Histonet] Cutting standards Message-ID: <9E956D8FEB06C2408B08AC16498325E9255DC6@scopemx1.interscope.com> Hi Rene, I am interested in reading this survey. Please send me a copy. Thank you. Janet Baez Interscope Pathology Medical Group -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 27, 2010 8:28 AM To: histonet@lists.utsouthwestern.edu; histotech@imagesbyhopper.com Subject: Re: [Histonet] Cutting standards The averages from a survey of 325 histolabs is 50 blocks/hour to embed and 24 blocks/hour to cut. If interested I can send you an article about this. Ren? J. --- On Fri, 8/27/10, histotech@imagesbyhopper.com wrote: From: histotech@imagesbyhopper.com Subject: [Histonet] Cutting standards To: histonet@lists.utsouthwestern.edu Date: Friday, August 27, 2010, 9:50 AM I know this question has been asked before ... Can anyone share with me what they are actually using as a cutting/embedding standard for your techs?? For instance, how many seconds (mins?) do you allow for embedding a block?? How many seconds(mins?) do you allow for cutting a block? For simplicity here, I am looking at the "plop and drop" type specimens, ie larger specimens that don't require specific orientation and can be placed in a mold easily.? These types of blocks will generally have one section on one slide.? I am trying to find out if the standard I have for my techs is too tough or too lenient on them.? I allow 45 seconds to embed such a block and another 45 seconds to section that same block. How does that fit with what you guys are all doing? Thanks! Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Fri Aug 27 10:39:16 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Aug 27 10:39:21 2010 Subject: [Histonet] Cutting standards In-Reply-To: References: <5A2BD13465E061429D6455C8D6B40E390EAF72C789@IBMB7Exchange.digestivespecialists.com>, Message-ID: This question keeps coming up with increasing frequency. There have been a few articles trying to suggest standards, but because Histology is not a "standardized" process, you must develop your own standards in your lab. I suggest the minimum set of variables you need to take into account are: specimen type mix; grossing protocols (number/amount of tissue placed in a cassette); embedding protocols (how tissue is placed in mold); experience of techs (include everyone performing the task) You will need to do a time study that will cover all techs and over several days. Don't use average, use mean. Set the mean as the minimum performance expectation and standard, re-time the task anytime one or more of the variables changes, combine performance with your quality standard and adjust minimum standard accordingly. If you set standards in your lab, you must make sure of two important factors. First, make the standard fair and equitable for all. Second, make sure your standards meet and support your production and quality needs. William DeSalvo, B.S., HTL(ASCP) > From: histotech@imagesbyhopper.com > To: lblazek@digestivespecialists.com; sgoebel@xbiotech.com > Date: Fri, 27 Aug 2010 11:10:32 -0400 > Subject: RE: [Histonet] Cutting standards > CC: histonet@lists.utsouthwestern.edu > > :o) Okay, to clear something up, I am using the times as a AVERAGE for > cutting and embedding over the course of the day. I do understand that > there are easy blocks and difficult ones and the average between them! If I > am to tell a tech they could work faster/more efficiently etc, I would feel > better if I have some sort of a standard goal which they can aim for, hence > the reason for my question. > > And no, I'm not being hard core! ;o) CAP even asks if there are standards > set for your techs. > > I'm enjoying the discussion and thank you all for taking the time to help. > :o) > > Michelle > > > > -----Original Message----- > From: Blazek, Linda [mailto:lblazek@digestivespecialists.com] > Sent: Friday, August 27, 2010 10:31 AM > To: 'sgoebel@xbiotech.com'; histotech@imagesbyhopper.com > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Cutting standards > > > I'm up for the Margaritas! > I agree with the 45 seconds at cutting a block is not a good time measure. > I think that if you are including cleaning the edges of the block, facing > the block, making a slide for the block etc. then 2 minutes is a good > average. I think years and years ago that was what one of the time studies > I participated in figured was an average time. However, I don't think > timing someone that cutting is a very good practice with all of the > variables involved. I think rather than timing someone I would rather, if a > tech seems to be very slow, observing why that particular person is > significantly slower than others and see if there is a way to increase their > productivity. > My two cents worth! Now you have four cents! > > Now! Anyone looking for a job in the Dayton, Oh area? I have an opening > for a tech. We have a great team and state of the art equipment. > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Digestive Specialists, Inc > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > sgoebel@xbiotech.com > Sent: Friday, August 27, 2010 10:11 AM > To: histotech@imagesbyhopper.com > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Cutting standards > > > Wow, you time your techs!?! Hard core!!! 45 seconds t=mbed > sounds ok, but 45 seconds to section? What if the tissue need= > little extra care? Some are harder and some are fatty. If I=ly > had 45 seconds to cut a giant hunk of breast that would be alot of pr > essure!!! Just remember crap in crap out...sometimes you have to > take=ittle longer to do it right with one try instead of taking > extra time t=o something you rushed again. Just my two cents? > =) > > <=v>Happy Friday everyone...margaritas after work!!! > > Sarah Goebel, B.A., HT=SCP) > Histotechnician > XBiotech USA Inc. > 8201 East Riverside Dr. Bldg 4 Suite 100 > Austin, Tex= 78744 > (512)386-5107 > > -------- Original Message -------- > Subject: [Histonet] Cutting standards > From: <[1]histotech@imagesb=opper.com> > Date: Fri, August 27, 2010 6:50 am > To: <[2]histonet@lists=tsouthwestern.edu> > I know this question has been asked before ... Can anyone share with > me wha= > they are actually using as a cutting/embedding standard for your > techs? Fo= > instance, how many seconds (mins?) do you allow for embedding a block? > How many seconds(mins?) do you allow for cutting a block? > For simplicity here, I am looking at the "plop and drop" type > specimens, ie larger specimens that don't require specific > orientation and can be > placed<=> in a mold easily. These types of blocks will generally > have one section on one slide. I am trying to find out if the standard > I have for my techs > is<=> too tough or too lenient on them. I allow 45 seconds to embed > such a block and another 45 seconds to section that same block. > How does that fit with what you guys are all doing? > Thanks! > Michelle > _______________________________________________ > Histonet mailing list > [3]Histonet@lists.utsouth=stern.edu > [4]http:=lists.utsouthwestern.edu/mailman/listinfo/histonet > > References > > 1. 3D"mailto:histotech@imagesbyhopper.com" > 2. 3D"mailto:histonet@lists.utsouthwestern.edu" > 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" > 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.441 / Virus Database: 271.1.1/3097 - Release Date: 08/27/10 > 06:34:00 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Raoul.Regnault <@t> interiorhealth.ca Fri Aug 27 10:40:37 2010 From: Raoul.Regnault <@t> interiorhealth.ca (Regnault, Raoul) Date: Fri Aug 27 10:40:42 2010 Subject: [Histonet] Cleaning molds In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16403321606D9@CHEXCMS10.one.ads.che.org> References: <65365F35C0F2EF4D846EC3CA73E49C43D7F2094844@HPEMX3.HealthPartners.int> <791677523-1282839395-cardhu_decombobulator_blackberry.rim.net-1965674517-@bda104.bisx.prod.on.blackberry> <92AD9B20A6C38C4587A9FEBE3A30E16403321606D9@CHEXCMS10.one.ads.che.org> Message-ID: <6F9479965E01D04DA2EF4B6C341335680677078A6A@DC1SERV353.interiorhealth.ca> I agree with Joyce. Here we have neither cleaned regularly nor used mold release for decades - since the solid state cooling plate came into use. Raoul Regnault Anatomic Pathology Kootenay Boundary Regional Hospital 1200 Hospital Bench Trail B.C. V1R 4M1 250-368-3311 ext 2263 fax 250-364-3457 raoul.regnault@interiorhealth.ca ? Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, August 27, 2010 8:33 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cleaning molds If you keep your molds in the hot tray of the embedding center, you never have to clean them... I promise. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, August 27, 2010 09:53 To: chapcl@yahoo.com; Webb, Dorothy L; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cleaning molds yeah, I'd be careful of this method. We used to do this at the U.S. Air Force hospital I used to work at when I was active duty. One time, the fire alarm went off and we had to evacuate the lab. Well, there must have been a real problem because we were out of the lab a long time. By the time we got the "all clear" to go back into the lab, I was the first one back and ran into some firemen. Seems the water boiled out of the pot and the paraffin was smoking up a storm.. No one said anything, but I bet that was why we were out of the lab so long. Now, those of you that have worked in a military or government hospital realize all the freaking paperwork that had to be filled out from that one incident. We were ordered by Safety to develop another method. So, we soak our molds in xylene for a couple of hours, covered of course as not expose the lab to the xylene fumes (that would required addition freaking paperwork) followed by a 100% etoh rinse, followed by air drying. JTT ----- Original Message ----- From: To: "Webb, Dorothy L" ; ; Sent: Thursday, August 26, 2010 11:16 AM Subject: Re: [Histonet] Cleaning molds > Back when I was using metal cassette lids and an autotechnicon (so no > cleaning in the processor) I would throw all the lids and molds into a > pot after embedding. Throw a bit of laboratory detergent in, fill with > water, and boil on a hot plate. Once I got to a rolling boil, I would > turn the heat off. By the end of the day it was cool. Peel the > paraffin off the top, then rinse the molds. > > Worked great! Didn't even need mold release. > > Will > > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: "Webb, Dorothy L" > Sender: histonet-bounces@lists.utsouthwestern.edu > Date: Thu, 26 Aug 2010 10:53:58 > To: > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Cleaning molds > > Does anyone in histoland clean their embedding molds in a dishwasher? > Otherwise, besides placing in the cleaning cycle of your processor, > how do sites clean their molds?? Simple, but plaguing question!!!!!!! > Thanks all! > > Dorothy Webb > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, > please be advised that you have received this e-mail in error and that > any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Fri Aug 27 10:56:16 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Aug 27 10:56:21 2010 Subject: [Histonet] Specimen Retention Message-ID: <57BE698966D5C54EAE8612E8941D768309722393@EXCHANGE3.huntingtonhospital.com> CAP states that all gross specimens and wet tissue be retained until at least 2 weeks after the final reports are reported out. Do others follow these guidelines for hardware, foreign bodies, etc, or would you possibly return them to the patient sooner than the 2 weeks? Laurie Colbert From rsrichmond <@t> gmail.com Fri Aug 27 11:03:18 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Aug 27 11:03:32 2010 Subject: [Histonet] Re: Rapid liver core biopsy processing Message-ID: Garth Fraga, a pathologist at the University of Kansas Medical Center asks: >>We do about a hundred liver transplants/year at our hospital, and the hepatologists do lots of liver core biopsies to rule out rejection. They want a same-day diagnosis on these, so historically they have been rush processed on a two-hour cycle (VIP machine). They are brought over directly from the liver biopsy suite immediately after biopsy, so they get very little fixation in the specimen container prior to going on the processor. Recently we had a couple of sub-par cases and have moved to a four-hour processing cycle. Do any of you have any experience dealing with rush-processed liver cores in transplant patients? What sort of a processing schedule do you recommend? Anyone handling them like frozen sections?<< I would address this question to the pathologists at the University of Pittsburgh, since this is the foremost liver transplant service possibly in the world. I've consulted their Web site about liver transplants - quite a while ago - and it was quite helpful. Garth, if you get a reply, could you post it on Histonet for us all to see? Bob Richmond Samurai Pathologist Knoxville TN From Bill.Tench <@t> pph.org Fri Aug 27 11:12:12 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Fri Aug 27 11:12:23 2010 Subject: [Histonet] gross specimen retention Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5569@MAIL1.pph.local> We generally do not return specimens to patients, so this is not so much of an issue for us. We do retain processed specimens longer than 2 weeks (more like 2 months). Fresh placentas (requiring refrigerated storage) are an exception. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From LSebree <@t> uwhealth.org Fri Aug 27 11:14:32 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Aug 27 11:14:38 2010 Subject: [Histonet] Re: Rapid liver core biopsy processing In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E2738399F8B@UWHC-MAIL01.uwhis.hosp.wisc.edu> Why not do C4d on frozen sections? That's what we do for our renal bxs to check for rejection. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Friday, August 27, 2010 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Rapid liver core biopsy processing Garth Fraga, a pathologist at the University of Kansas Medical Center asks: >>We do about a hundred liver transplants/year at our hospital, and the >>hepatologists do lots of liver core biopsies to rule out rejection. >>They want a same-day diagnosis on these, so historically they have >>been rush processed on a two-hour cycle (VIP machine). They are >>brought over directly from the liver biopsy suite immediately after >>biopsy, so they get very little fixation in the specimen container >>prior to going on the processor. Recently we had a couple of sub-par >>cases and have moved to a four-hour processing cycle. Do any of you >>have any experience dealing with rush-processed liver cores in >>transplant patients? What sort of a processing schedule do you >>recommend? Anyone handling them like frozen sections?<< I would address this question to the pathologists at the University of Pittsburgh, since this is the foremost liver transplant service possibly in the world. I've consulted their Web site about liver transplants - quite a while ago - and it was quite helpful. Garth, if you get a reply, could you post it on Histonet for us all to see? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Aug 27 11:52:43 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Aug 27 11:52:57 2010 Subject: [Histonet] FITC on Ventana Ultra In-Reply-To: <4C7785AB.5D38.00EF.0@swmail.sw.org> References: <4C7785AB.5D38.00EF.0@swmail.sw.org> Message-ID: <1AAF670737F193429070841C6B2ADD4C0255010090@EXMBMCB15.ucsfmedicalcenter.org> Part of the Ventana response was "Many customers, however have expressed that they would rather utilize the slide drawer for another patient slide, rather than running the fitc negative." I hope the customers come to understand that the negative control is necessary, not just an option. Also, it has come to light that it is difficult to run lot comparison tests on some automated instruments as CAP is now requiring. From Ventana the information I have gotten is that the customer has to "trick" the system to be able to test two lots in one run. Two lots of the same antibody can be used on one run, but the system will use the oldest lot first, then switch to the new lot when the old lot runs out. So to do a lot comparison the customer must arrange to use the last test from the old lot on one slide and then assume the system will switch to the new lot for the other slide. Hopefully it works. I don't use Ventana but am interested because we are looking at the system, plus I am giving a validation workshop at NSH and one of the most common questions now is how to do a lot comparison test on an automated system. I welcome any other information on that process on any system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, August 27, 2010 7:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FITC on Ventana Ultra Any users out there that have had A CAP inspector question negative control on the instrument? In regard to CAP question ANP.21850 in which the notes state, "A negative reagent control in which the patient tissue is processed in an identical manner to the test specimen but with the primary antibody omitted must be performed for each patient test specimen?" Below is the response from Ventana regarding "negatives" I am curios if CAP accepts these methods?? In regards to the question below about running a negative control fitc, there are two ways to accomplish this. 1) There is not currently a place in the protocol to select a fitc negative. We can still be compliant by ensuring the negative slide is treated the same as the patient. The only reagent the patient slide is exposed to, besides the fitc antibody, is reaction buffer. Running a negative can be accomplished by applying reaction buffer to the slide and letting it incubate for the same amount of time. Next, it is important to ensure both slides are coverslipped in the came mounting media. 2) The second method is to utilize internal negatives that are already present within the patient tissue. However, this is difficult when running Fitc antibodies such as IgG, IgM, etc. We are looking into adding this addition into the software. Many customers, however have expressed that they would rather utilize the slide drawer for another patient slide, rather than running the fitc negative. Am interested in your comments. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 From kalschev <@t> svm.vetmed.wisc.edu Fri Aug 27 11:57:28 2010 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Fri Aug 27 11:58:03 2010 Subject: [Histonet] Goldner's T. for plastic grd. tissue on EXAKT slides-quick procedure Message-ID: <36B7C9C3E8A741A9B8C6F828C08A5883@vetmed.wisc.edu> I generally use the Goldner's Stain on free-floating ground or microtomed sections. The procedure is not working on the current project. This project with titanium inplants looks better polished on the Exakt. Does anyone have a Goldner's Trichrome they can share for Exakt Grd. sections/100 micron? Detailed please. Feel free to fax. A good weekend all. VK Vicki Kalscheur Phone: 608-262-8534 FAX: 608-263-7930 kalschev@svm.vetmed.wisc.edu From marktarango <@t> gmail.com Fri Aug 27 12:18:07 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Aug 27 12:19:32 2010 Subject: [Histonet] FITC on Ventana Ultra In-Reply-To: <4C7785AB.5D38.00EF.0@swmail.sw.org> References: <4C7785AB.5D38.00EF.0@swmail.sw.org> Message-ID: Hi Nita, I agree that buffer should be used as the negative control reagent. If you have a seperate slide that is cut and put into buffer, just coverslip it with the same mounting media and you have a negative control. All the instrument is doing is putting on the antibody and then rinsing it off. So your negative control is a slide that didn't get the antibody (just buffer). Makes sense to me. If you get ventana to add a negative control to their software it would do just the same thing as coverslipping from buffer, but you'd be paying ventana for it. Mark On Fri, Aug 27, 2010 at 7:30 AM, Nita Searcy wrote: > Any users out there that have had A CAP inspector question negative control > on the instrument? In regard to CAP question ANP.21850 in which the notes > state, "A negative reagent control in which the patient tissue is processed > in an identical manner to the test specimen but with the primary antibody > omitted must be performed for each patient test specimen?" > > Below is the response from Ventana regarding "negatives" I am curios if CAP > accepts these methods?? > > In regards to the question below about running a negative control fitc, > there are two ways to accomplish this. > > > > 1) There is not currently a place in the protocol to select a fitc > negative. We can still be compliant by ensuring the negative slide is > treated the same as the patient. The only reagent the patient slide is > exposed to, besides the fitc antibody, is reaction buffer. Running a > negative can be accomplished by applying reaction buffer to the slide and > letting it incubate for the same amount of time. Next, it is important to > ensure both slides are coverslipped in the came mounting media. > 2) The second method is to utilize internal negatives that are already > present within the patient tissue. However, this is difficult when running > Fitc antibodies such as IgG, IgM, etc. > We are looking into adding this addition into the software. Many customers, > however have expressed that they would rather utilize the slide drawer for > another patient slide, rather than running the fitc negative. > > Am interested in your comments. > Thanks > > > > > > Nita Searcy, HT/HTL (ASCP) > Scott and White Hospital > Division Manager, Anatomic Pathology > 2401 S. 31st. Street > 254-724-2438 > Temple, Texas, 76502 > nsearcy@swmail.sw.org > > > 254-724-2438 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From BSullivan <@t> shorememorial.org Fri Aug 27 12:18:43 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Fri Aug 27 12:21:20 2010 Subject: [Histonet] Specimen Retention In-Reply-To: <57BE698966D5C54EAE8612E8941D768309722393@EXCHANGE3.huntingtonhospital.com> Message-ID: We keep all tissue sent to us for the required time . This includes hardware and foreign bodies etc., etc. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Laurie Colbert" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Specimen Retention 08/27/2010 11:56 AM CAP states that all gross specimens and wet tissue be retained until at least 2 weeks after the final reports are reported out. Do others follow these guidelines for hardware, foreign bodies, etc, or would you possibly return them to the patient sooner than the 2 weeks? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From deannal78 <@t> verizon.net Fri Aug 27 12:28:04 2010 From: deannal78 <@t> verizon.net (Deanna Rhoads) Date: Fri Aug 27 12:28:08 2010 Subject: [Histonet] Re: Rapid liver core biopsy processing In-Reply-To: References: Message-ID: <671126.93121.qm@web84305.mail.re1.yahoo.com> I worked at the University of Pittsburgh Medical Center and we did the liver biopsies on a stat, 2 hour run, on the Leica Peloris.? We got great results and quick diagnosis. Deanna Rhoads HT (ASCP) Pittsburgh, PA ________________________________ From: Robert Richmond To: histonet@lists.utsouthwestern.edu Sent: Fri, August 27, 2010 12:03:18 PM Subject: [Histonet] Re: Rapid liver core biopsy processing Garth Fraga, a pathologist at the University of Kansas Medical Center asks: >>We do about a hundred liver transplants/year at our hospital, and the >>hepatologists do lots of liver core biopsies to rule out rejection. They want a >>same-day diagnosis on these, so historically they have been rush processed on a >>two-hour cycle (VIP machine). They are brought over directly from the liver >>biopsy suite immediately after biopsy, so they get very little fixation in the >>specimen container prior to going on the processor. Recently we had a couple of >>sub-par cases and have moved to a four-hour processing cycle. Do any of you have >>any experience dealing with rush-processed liver cores in transplant patients? >>What sort of a processing schedule do you recommend?? Anyone handling them like >>frozen sections?<< I would address this question to the pathologists at the University of Pittsburgh, since this is the foremost liver transplant service possibly in the world. I've consulted their Web site about liver transplants - quite a while ago - and it was quite helpful. Garth, if you get a reply, could you post it on Histonet for us all to see? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Emily.Kasprowicz <@t> hcmed.org Fri Aug 27 13:15:40 2010 From: Emily.Kasprowicz <@t> hcmed.org (Kasprowicz, Emily E) Date: Fri Aug 27 13:18:47 2010 Subject: FW: [Histonet] FITC on Ventana Ultra In-Reply-To: <66FFB482DE2B624DA30B826C179538214E3AAF2644@MCD-MBX-3.HCMC.CO.HENNEPIN.MN.US> References: <4C7785AB.5D38.00EF.0@swmail.sw.org>, <1AAF670737F193429070841C6B2ADD4C0255010090@EXMBMCB15.ucsfmedicalcenter.org>, <66FFB482DE2B624DA30B826C179538214E3AAF2644@MCD-MBX-3.HCMC.CO.HENNEPIN.MN.US> Message-ID: <66FFB482DE2B624DA30B826C179538214E3AAF2645@MCD-MBX-3.HCMC.CO.HENNEPIN.MN.US> Emily Kasprowicz, HT (ASCP) Histology Lab Hennepin County Medical Center 701 Park Avenue Minneapolis, MN 55415 612-873-3079 ________________________________________ From: Kasprowicz, Emily E Sent: Friday, August 27, 2010 1:13 PM To: Morken, Tim Subject: RE: [Histonet] FITC on Ventana Ultra We have a Benchmark XT and LT, and this has been somewhat of a nuisance, but not as much as the procedure that you have described. We, too, have to trick the system. What we have come up with (however, we have not been inspected by CAP since implementing this, so I don't know if this will be acceptable to the inspector) is to make a "titer" protocol for each of our antibodies. The protocol is exactly the same as our normal run, but we have to manually apply the antibody to the correct slide (we label our test slides with the lot numbers and "Old Lot" and "New Lot") at the time of antibody incubation. This was the least complicated way we could come up with to do the "side by side" testing required by CAP, and it seems to work pretty well. Our problem is is that we have to wait for a time when one of the machines is totally free so we can do the titer run. This is, of course, not very often, so we have to try to do lot comparisons as far in advance as possible. If we had the Ventana Ultra, it would be a little easier as we wouldn't have to hold up the entire machine for a Titer Run. We could just run the slides as needed. If anyone else has a better way to do lot checks on the Ventana Benchmarks, I'd be interested in hearing your procedure as well. Thanks, Emily Kasprowicz, HT (ASCP) Histology Lab Hennepin County Medical Center 701 Park Avenue Minneapolis, MN 55415 612-873-3079 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim [Timothy.Morken@ucsfmedctr.org] Sent: Friday, August 27, 2010 11:52 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FITC on Ventana Ultra Part of the Ventana response was "Many customers, however have expressed that they would rather utilize the slide drawer for another patient slide, rather than running the fitc negative." I hope the customers come to understand that the negative control is necessary, not just an option. Also, it has come to light that it is difficult to run lot comparison tests on some automated instruments as CAP is now requiring. From Ventana the information I have gotten is that the customer has to "trick" the system to be able to test two lots in one run. Two lots of the same antibody can be used on one run, but the system will use the oldest lot first, then switch to the new lot when the old lot runs out. So to do a lot comparison the customer must arrange to use the last test from the old lot on one slide and then assume the system will switch to the new lot for the other slide. Hopefully it works. I don't use Ventana but am interested because we are looking at the system, plus I am giving a validation workshop at NSH and one of the most common questions now is how to do a lot comparison test on an automated system. I welcome any other information on that process on any system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, August 27, 2010 7:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FITC on Ventana Ultra Any users out there that have had A CAP inspector question negative control on the instrument? In regard to CAP question ANP.21850 in which the notes state, "A negative reagent control in which the patient tissue is processed in an identical manner to the test specimen but with the primary antibody omitted must be performed for each patient test specimen?" Below is the response from Ventana regarding "negatives" I am curios if CAP accepts these methods?? In regards to the question below about running a negative control fitc, there are two ways to accomplish this. 1) There is not currently a place in the protocol to select a fitc negative. We can still be compliant by ensuring the negative slide is treated the same as the patient. The only reagent the patient slide is exposed to, besides the fitc antibody, is reaction buffer. Running a negative can be accomplished by applying reaction buffer to the slide and letting it incubate for the same amount of time. Next, it is important to ensure both slides are coverslipped in the came mounting media. 2) The second method is to utilize internal negatives that are already present within the patient tissue. However, this is difficult when running Fitc antibodies such as IgG, IgM, etc. We are looking into adding this addition into the software. Many customers, however have expressed that they would rather utilize the slide drawer for another patient slide, rather than running the fitc negative. Am interested in your comments. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.thotakura <@t> imperial.ac.uk Fri Aug 27 13:48:15 2010 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Fri Aug 27 13:49:52 2010 Subject: [Histonet] UNFIXED LIVER frozen SECTIONS Message-ID: Dear All, I want to make liver frozen sections but I don't want to fix the tissue. Once I make the section can I store the slides in -80 and fix them just before doing the staining?. Can you guys please suggest how to make unfixed liver frozen sections, how to store and process the tissue. Thank you very much for your help. Many Thanks, Anil Kumar. From thomas.crowell <@t> novartis.com Fri Aug 27 14:30:35 2010 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Fri Aug 27 14:30:41 2010 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 08/27/2010 and will not return until 09/07/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From Janice.Mahoney <@t> alegent.org Fri Aug 27 14:30:50 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Aug 27 14:31:02 2010 Subject: [Histonet] Ventana vs Leica In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A448@EXCHMBC2.ad.ah.local>, Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7EC969E@EXCHMBC2.ad.ah.local> Our Techs load all the reagents and assure that the slides are labeled properly. With Ventana it is so simple to load the slides, they can go in any order so there are no mix ups. Each slide has a bar code which matches the protocol. I would caution you when looking at pricing. I have heard that if you have one slide in one of the modules on the Bond, that it needs enough fluid for 5 slides to activate the pump. That would be wasting 4/5 of the fluids needed for that module. Sounds like a-lot of waste to me. I would ask about this for sure. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histotech@imagesbyhopper.com [histotech@imagesbyhopper.com] Sent: Thursday, August 26, 2010 7:21 PM To: Mahoney,Janice A Cc: BSullivan@shorememorial.org; JayLundgren; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Unless someone corrects me (or even agrees with me!) in FL only a technologist is allowed to load the IHC machine, so no additional lean for us. :o( I would be interested to hear more about the savings though, as we are preparing to be in the market for a new IHC machine. We currently have the Benchmark XT. On Aug 26, 2010, at 10:08 AM, "Mahoney,Janice A" wrote: > We love our Ventana instruments too Jay. I don't quite believe the 40% difference in cost. I'd like to see those numbers. I know I save in tech time and the instruments are very easy to use. We have histo assistants and secretaries trained to load and unload the instruments, saving out techs to do the things only techs can do. Talk about LEAN! > A little more from a LEAN perspective, the Ultra is the only instrument out there that is "TRUE" continuous flow. As soon as there is an open spot on the instrument and the antibody is on board, I can add a slide. I don't have to wait till one of the 10 slide modules is finished. Leica is still a batch instrument, it is just smaller batches than the older IHC models. I'm not putting Leica down, it is a fine instrument but I think it is important for people to know the facts. > Jan Mahoney > Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org > Sent: Tuesday, August 24, 2010 2:05 PM > To: Jay Lundgren > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Ventana vs Leica > > Jay, > I currently use the Ventana and am very pleased with the results I get. > The only draw back is the cost to run the instrument. It can get quite > pricey. They added space on the antibody wheel but took space away from the > slide area. This has impacted our work flow greatly. We are however looking > to purchase a second one. This one will have continual through put. That > should help out with TAT. Hope this helps. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > > Jay Lundgren > l.com> To > Sent by: histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] Ventana vs Leica > 08/24/2010 02:30 > PM > > > > > > > > > I was wondering if anyone out there had experience with both the > Ventana Ultra and the Leica Bond immunostainers. I realize that most > people > have a personal preference as to brands, but I'm not looking for a > knee-jerk > opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had > actual > experience working on a daily basis with both instruments. If this is you, > could you please tell me which you preferred and why. > I'm currently working for a facility in MT which has narrowed down its > search to these two instruments. No vendors please, they've already given > their pitches. > > Thanks, > Jay A. > Lundgren M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From thomasebrooks <@t> aol.com Fri Aug 27 14:34:43 2010 From: thomasebrooks <@t> aol.com (Thomas) Date: Fri Aug 27 14:35:06 2010 Subject: [Histonet] Cutting standards Message-ID: <3s91oeduftdgdyxrqmw3awqw.1282937683422@email.android.com> "Baez, Janet" wrote: >Hi Rene, > >I am interested in reading this survey. Please send me a copy. > >Thank you. >Janet Baez >Interscope Pathology Medical Group > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >Sent: Friday, August 27, 2010 8:28 AM >To: histonet@lists.utsouthwestern.edu; histotech@imagesbyhopper.com >Subject: Re: [Histonet] Cutting standards > >The averages from a survey of 325 histolabs is 50 blocks/hour to embed and 24 blocks/hour to cut. >If interested I can send you an article about this. >Ren? J. > >--- On Fri, 8/27/10, histotech@imagesbyhopper.com wrote: > > >From: histotech@imagesbyhopper.com >Subject: [Histonet] Cutting standards >To: histonet@lists.utsouthwestern.edu >Date: Friday, August 27, 2010, 9:50 AM > > >I know this question has been asked before ... Can anyone share with me what >they are actually using as a cutting/embedding standard for your techs?? For >instance, how many seconds (mins?) do you allow for embedding a block?? How >many seconds(mins?) do you allow for cutting a block? > >For simplicity here, I am looking at the "plop and drop" type specimens, ie >larger specimens that don't require specific orientation and can be placed >in a mold easily.? These types of blocks will generally have one section on >one slide.? I am trying to find out if the standard I have for my techs is >too tough or too lenient on them.? I allow 45 seconds to embed such a block >and another 45 seconds to section that same block. > >How does that fit with what you guys are all doing? > >Thanks! > >Michelle > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Fri Aug 27 15:16:56 2010 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Aug 27 15:17:18 2010 Subject: [Histonet] seeking part time histology job in Ann Arbor, MI Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB4011374407D8E@CVMMBX.vetmed.wsu.edu> Hi All, My coworker, Joy, is moving to Ann Arbor in Oct. and would be interested in part time histology or pharmacy aide work while she attends classes. She has been working 7 years in fluorescent and automated IHC in a research setting. In China, she also obtained her PHD in Chinese Medicine. I can give you her contact info, if you know of a position. Thankyou, Tom Truscott From histotech <@t> imagesbyhopper.com Sat Aug 28 09:33:57 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Aug 28 09:34:06 2010 Subject: [Histonet] Ventana vs Leica In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7EC969E@EXCHMBC2.ad.ah.local> Message-ID: <9BA66B182D6448DB8B2382CCB1DD9DF0@hopperPC> Is there anyone in FL who can confirm (or refute) my thoughts on whether or not a lab aide can load the IHC machine if a tech set up the labels for the automated staining? And if they can load it, is there any documentation to support this, just to keep my lab honest? Thanks! Michelle -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, August 27, 2010 3:31 PM To: histotech@imagesbyhopper.com Cc: BSullivan@shorememorial.org; JayLundgren; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana vs Leica Our Techs load all the reagents and assure that the slides are labeled properly. With Ventana it is so simple to load the slides, they can go in any order so there are no mix ups. Each slide has a bar code which matches the protocol. I would caution you when looking at pricing. I have heard that if you have one slide in one of the modules on the Bond, that it needs enough fluid for 5 slides to activate the pump. That would be wasting 4/5 of the fluids needed for that module. Sounds like a-lot of waste to me. I would ask about this for sure. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histotech@imagesbyhopper.com [histotech@imagesbyhopper.com] Sent: Thursday, August 26, 2010 7:21 PM To: Mahoney,Janice A Cc: BSullivan@shorememorial.org; JayLundgren; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Unless someone corrects me (or even agrees with me!) in FL only a technologist is allowed to load the IHC machine, so no additional lean for us. :o( I would be interested to hear more about the savings though, as we are preparing to be in the market for a new IHC machine. We currently have the Benchmark XT. On Aug 26, 2010, at 10:08 AM, "Mahoney,Janice A" wrote: > We love our Ventana instruments too Jay. I don't quite believe the > 40% difference in cost. I'd like to see those numbers. I know I save > in tech time and the instruments are very easy to use. We have histo > assistants and secretaries trained to load and unload the instruments, > saving out techs to do the things only techs can do. Talk about LEAN! > A little more from a LEAN perspective, the Ultra is the only > instrument out there that is "TRUE" continuous flow. As soon as there > is an open spot on the instrument and the antibody is on board, I can > add a slide. I don't have to wait till one of the 10 slide modules is > finished. Leica is still a batch instrument, it is just smaller > batches than the older IHC models. I'm not putting Leica down, it is a > fine instrument but I think it is important for people to know the > facts. Jan Mahoney Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org > Sent: Tuesday, August 24, 2010 2:05 PM > To: Jay Lundgren > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Ventana vs Leica > > Jay, > I currently use the Ventana and am very pleased with the results I > get. The only draw back is the cost to run the instrument. It can get > quite pricey. They added space on the antibody wheel but took space > away from the slide area. This has impacted our work flow greatly. We > are however looking to purchase a second one. This one will have > continual through put. That should help out with TAT. Hope this helps. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > > Jay Lundgren > l.com> To > Sent by: histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] Ventana vs Leica > 08/24/2010 02:30 > PM > > > > > > > > > I was wondering if anyone out there had experience with both the > Ventana Ultra and the Leica Bond immunostainers. I realize that most > people have a personal preference as to brands, but I'm not looking > for a knee-jerk > opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had > actual > experience working on a daily basis with both instruments. If this is you, > could you please tell me which you preferred and why. > I'm currently working for a facility in MT which has narrowed down its > search to these two instruments. No vendors please, they've already given > their pitches. > > Thanks, > Jay A. > Lundgren M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health > is faithful to the healing ministry of Jesus Christ, providing high > quality care for the body, mind and spirit of every person. > > The information contained in this communication, including > attachments, is confidential and private and intended only for the use > of the addressees. Unauthorized use, disclosure, distribution or > copying is strictly prohibited and may be unlawful. If you received > this communication in error, please inform us of the erroneous > delivery by return e-mail message from your computer. Additionally, > although all attachments have been scanned at the source for viruses, > the recipient should check any attachments for the presence of viruses > before opening. Alegent Health accepts no liability for any damage > caused by any virus transmitted by this e-mail. Thank you for your > cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3097 - Release Date: 08/27/10 06:34:00 From tahseen <@t> brain.net.pk Sun Aug 29 07:32:58 2010 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Sun Aug 29 07:33:09 2010 Subject: [Histonet] TMA sectioning problam Message-ID: <22348.119.152.13.78.1283085178.squirrel@brain.net.pk> Hi All, We have problem with TMA sectioning. What we did, 1 Put 38 tissues in 60 wholes TMA block. 2 Invert block on slide 40C for 15 mints. 3 Put on Ice to cold and remove slide. 4 On Microtome sectioning we found no tissue on slide while all tissues are seen in the wholes with smooth surface. Thanks Muhammad Tahseen Senior Supervisor Histopathology SKMCH&RC Lahore Pakistan From graceofgod011978 <@t> hotmail.com Sun Aug 29 19:52:42 2010 From: graceofgod011978 <@t> hotmail.com (Emmanuel O) Date: Sun Aug 29 19:52:46 2010 Subject: [Histonet] Looking for new position Message-ID: Hi, I am a certified HISTOLOGIST and looking for a new position as either a bech histotech. I am open to any shift and relocation.. Emmanuel. From AnthonyH <@t> chw.edu.au Sun Aug 29 21:07:00 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 29 21:07:46 2010 Subject: [Histonet] Rapid liver core biopsy processing In-Reply-To: <4C765C310200001B00064A21@smtpout.kumc.edu> Message-ID: Garth, We have two methods available: The first is a microwave method using the Milestone Mega TT. It uses 10% NBF (25min at 35oC followed by 25min at 50oC), followed by an ethanol rinse (2-3min room temp), 7min at 69oC in isopropanol and finally 8 min at 79oC in wax. The second uses our routine processor, 1hr in NBF at 40oC, followed by ethanol 10min x 4, xylene 10min x3, wax 10min x3. I have found it important to extend the NBF fixation step as much as I can - gives better results. Also these methods apply to core biopsies or very thin slices (<1mm) Hope this helps Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garth Fraga Sent: Friday, 27 August 2010 3:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rapid liver core biopsy processing Dear histonetters, We do about a hundred liver transplants/year at our hospital, and the hepatologists do lots of liver core biopsies to rule out rejection. They want a same-day diagnosis on these, so historically they have been rush processed on a two-hour cycle (VIP machine). They are brought over directly from the liver biopsy suite immediately after biopsy, so they get very little fixation in the specimen container prior to going on the processor. Recently we had a couple of sub-par cases and have moved to a four-hour processing cycle. Do any of you have any experience dealing with rush-processed liver cores in transplant patients? What sort of a processing schedule do you recommend? Anyone handling them like frozen sections? Thanks, Garth Fraga (pathologist) University of Kansas Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Rhonda.Hartz <@t> saskatoonhealthregion.ca Sun Aug 29 23:41:29 2010 From: Rhonda.Hartz <@t> saskatoonhealthregion.ca (Hartz, Rhonda SktnHR) Date: Sun Aug 29 23:41:37 2010 Subject: [Histonet] (no subject) Message-ID: I had submitted a please unsubscribe email over a week ago. I am still receiving emails. PLEASE UNSUBSCRIBE. Thank you. Rhonda Hartz From lpwenk <@t> sbcglobal.net Mon Aug 30 04:28:04 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Aug 30 04:28:07 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: Look at the bottom of any email from Histonet. The 2nd link says "lists.utsouthwestern" - click on it. At the bottom of the page is directions on how to unsubscribe. Follow the directions. Peggy Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Hartz, Rhonda SktnHR" Sent: Monday, August 30, 2010 12:41 AM To: Subject: [Histonet] (no subject) > I had submitted a please unsubscribe email over a week ago. I am still > receiving emails. PLEASE UNSUBSCRIBE. > > Thank you. > > Rhonda Hartz > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathology01 <@t> rccltd.in Mon Aug 30 06:07:22 2010 From: pathology01 <@t> rccltd.in (Pathology) Date: Mon Aug 30 06:06:41 2010 Subject: [Histonet] (no subject) Message-ID: <000001cb4833$881683e0$98438ba0$@in> Hi , How to avoid vacuoles in the sections of brain and testes of rats. Whether it is the problem of fixing, processing or staining. Thanks & Regards, S.Naveen Babu Technician - Pathology From pathology01 <@t> rccltd.in Mon Aug 30 06:13:27 2010 From: pathology01 <@t> rccltd.in (Pathology) Date: Mon Aug 30 06:12:56 2010 Subject: [Histonet] RE: Histonet Digest, Vol 81, Issue 39 Message-ID: <000501cb4834$62d8b660$288a2320$@in> Hi Reni, I am interested in reading this survey. Please send me a copy. Thanks & Regards, S.Naveen Babu Technician - Pathology Genome Valley, Shameerpet, Hyderabad - 500 078. A.P. India. Tel.: +91 40 2348 0422 Fax: +91 40 2348 0420? www.rccltd.in ? ? Disclaimer Information transmitted by this E-MAIL is proprietary to RCC Laboratories India Pvt. Ltd. and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: FITC on Ventana Ultra (Mark Tarango) 2. Re: Specimen Retention (BSullivan@shorememorial.org) 3. Re: Re: Rapid liver core biopsy processing (Deanna Rhoads) 4. FW: [Histonet] FITC on Ventana Ultra (Kasprowicz, Emily E) 5. UNFIXED LIVER frozen SECTIONS (Thotakura, Anil Kumar) 6. Thomas Crowell is out of the office. (thomas.crowell@novartis.com) 7. RE: Ventana vs Leica (Mahoney,Janice A) 8. RE: Cutting standards (Thomas) 9. seeking part time histology job in Ann Arbor, MI (Truscott, Tom) 10. RE: Ventana vs Leica (histotech@imagesbyhopper.com) ---------------------------------------------------------------------- Message: 1 Date: Fri, 27 Aug 2010 10:18:07 -0700 From: Mark Tarango Subject: Re: [Histonet] FITC on Ventana Ultra To: Nita Searcy Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Nita, I agree that buffer should be used as the negative control reagent. If you have a seperate slide that is cut and put into buffer, just coverslip it with the same mounting media and you have a negative control. All the instrument is doing is putting on the antibody and then rinsing it off. So your negative control is a slide that didn't get the antibody (just buffer). Makes sense to me. If you get ventana to add a negative control to their software it would do just the same thing as coverslipping from buffer, but you'd be paying ventana for it. Mark On Fri, Aug 27, 2010 at 7:30 AM, Nita Searcy wrote: > Any users out there that have had A CAP inspector question negative control > on the instrument? In regard to CAP question ANP.21850 in which the notes > state, "A negative reagent control in which the patient tissue is processed > in an identical manner to the test specimen but with the primary antibody > omitted must be performed for each patient test specimen?" > > Below is the response from Ventana regarding "negatives" I am curios if CAP > accepts these methods?? > > In regards to the question below about running a negative control fitc, > there are two ways to accomplish this. > > > > 1) There is not currently a place in the protocol to select a fitc > negative. We can still be compliant by ensuring the negative slide is > treated the same as the patient. The only reagent the patient slide is > exposed to, besides the fitc antibody, is reaction buffer. Running a > negative can be accomplished by applying reaction buffer to the slide and > letting it incubate for the same amount of time. Next, it is important to > ensure both slides are coverslipped in the came mounting media. > 2) The second method is to utilize internal negatives that are already > present within the patient tissue. However, this is difficult when running > Fitc antibodies such as IgG, IgM, etc. > We are looking into adding this addition into the software. Many customers, > however have expressed that they would rather utilize the slide drawer for > another patient slide, rather than running the fitc negative. > > Am interested in your comments. > Thanks > > > > > > Nita Searcy, HT/HTL (ASCP) > Scott and White Hospital > Division Manager, Anatomic Pathology > 2401 S. 31st. Street > 254-724-2438 > Temple, Texas, 76502 > nsearcy@swmail.sw.org > > > 254-724-2438 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 2 Date: Fri, 27 Aug 2010 13:18:43 -0400 From: BSullivan@shorememorial.org Subject: Re: [Histonet] Specimen Retention To: "Laurie Colbert" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII We keep all tissue sent to us for the required time . This includes hardware and foreign bodies etc., etc. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Laurie Colbert" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Specimen Retention 08/27/2010 11:56 AM CAP states that all gross specimens and wet tissue be retained until at least 2 weeks after the final reports are reported out. Do others follow these guidelines for hardware, foreign bodies, etc, or would you possibly return them to the patient sooner than the 2 weeks? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 27 Aug 2010 10:28:04 -0700 (PDT) From: Deanna Rhoads Subject: Re: [Histonet] Re: Rapid liver core biopsy processing To: Robert Richmond , histonet@lists.utsouthwestern.edu Message-ID: <671126.93121.qm@web84305.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I worked at the University of Pittsburgh Medical Center and we did the liver biopsies on a stat, 2 hour run, on the Leica Peloris. We got great results and quick diagnosis. Deanna Rhoads HT (ASCP) Pittsburgh, PA ________________________________ From: Robert Richmond To: histonet@lists.utsouthwestern.edu Sent: Fri, August 27, 2010 12:03:18 PM Subject: [Histonet] Re: Rapid liver core biopsy processing Garth Fraga, a pathologist at the University of Kansas Medical Center asks: >>We do about a hundred liver transplants/year at our hospital, and the >>hepatologists do lots of liver core biopsies to rule out rejection. They want a >>same-day diagnosis on these, so historically they have been rush processed on a >>two-hour cycle (VIP machine). They are brought over directly from the liver >>biopsy suite immediately after biopsy, so they get very little fixation in the >>specimen container prior to going on the processor. Recently we had a couple of >>sub-par cases and have moved to a four-hour processing cycle. Do any of you have >>any experience dealing with rush-processed liver cores in transplant patients? >>What sort of a processing schedule do you recommend? Anyone handling them like >>frozen sections?<< I would address this question to the pathologists at the University of Pittsburgh, since this is the foremost liver transplant service possibly in the world. I've consulted their Web site about liver transplants - quite a while ago - and it was quite helpful. Garth, if you get a reply, could you post it on Histonet for us all to see? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 27 Aug 2010 13:15:40 -0500 From: "Kasprowicz, Emily E" Subject: FW: [Histonet] FITC on Ventana Ultra To: "histonet@lists.utsouthwestern.edu" Message-ID: <66FFB482DE2B624DA30B826C179538214E3AAF2645@MCD-MBX-3.HCMC.CO.HENNEPIN.MN.US > Content-Type: text/plain; charset="us-ascii" Emily Kasprowicz, HT (ASCP) Histology Lab Hennepin County Medical Center 701 Park Avenue Minneapolis, MN 55415 612-873-3079 ________________________________________ From: Kasprowicz, Emily E Sent: Friday, August 27, 2010 1:13 PM To: Morken, Tim Subject: RE: [Histonet] FITC on Ventana Ultra We have a Benchmark XT and LT, and this has been somewhat of a nuisance, but not as much as the procedure that you have described. We, too, have to trick the system. What we have come up with (however, we have not been inspected by CAP since implementing this, so I don't know if this will be acceptable to the inspector) is to make a "titer" protocol for each of our antibodies. The protocol is exactly the same as our normal run, but we have to manually apply the antibody to the correct slide (we label our test slides with the lot numbers and "Old Lot" and "New Lot") at the time of antibody incubation. This was the least complicated way we could come up with to do the "side by side" testing required by CAP, and it seems to work pretty well. Our problem is is that we have to wait for a time when one of the machines is totally free so we can do the titer run. This is, of course, not very often, so we have to try to do lot comparisons as far in advance as possible. If we had the Ventana Ultra, it would be a little easier as we wouldn't have to hold up the entire machine for a Titer Run. We could just run the slides as needed. If anyone else has a better way to do lot checks on the Ventana Benchmarks, I'd be interested in hearing your procedure as well. Thanks, Emily Kasprowicz, HT (ASCP) Histology Lab Hennepin County Medical Center 701 Park Avenue Minneapolis, MN 55415 612-873-3079 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim [Timothy.Morken@ucsfmedctr.org] Sent: Friday, August 27, 2010 11:52 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FITC on Ventana Ultra Part of the Ventana response was "Many customers, however have expressed that they would rather utilize the slide drawer for another patient slide, rather than running the fitc negative." I hope the customers come to understand that the negative control is necessary, not just an option. Also, it has come to light that it is difficult to run lot comparison tests on some automated instruments as CAP is now requiring. From Ventana the information I have gotten is that the customer has to "trick" the system to be able to test two lots in one run. Two lots of the same antibody can be used on one run, but the system will use the oldest lot first, then switch to the new lot when the old lot runs out. So to do a lot comparison the customer must arrange to use the last test from the old lot on one slide and then assume the system will switch to the new lot for the other slide. Hopefully it works. I don't use Ventana but am interested because we are looking at the system, plus I am giving a validation workshop at NSH and one of the most common questions now is how to do a lot comparison test on an automated system. I welcome any other information on that process on any system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, August 27, 2010 7:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FITC on Ventana Ultra Any users out there that have had A CAP inspector question negative control on the instrument? In regard to CAP question ANP.21850 in which the notes state, "A negative reagent control in which the patient tissue is processed in an identical manner to the test specimen but with the primary antibody omitted must be performed for each patient test specimen?" Below is the response from Ventana regarding "negatives" I am curios if CAP accepts these methods?? In regards to the question below about running a negative control fitc, there are two ways to accomplish this. 1) There is not currently a place in the protocol to select a fitc negative. We can still be compliant by ensuring the negative slide is treated the same as the patient. The only reagent the patient slide is exposed to, besides the fitc antibody, is reaction buffer. Running a negative can be accomplished by applying reaction buffer to the slide and letting it incubate for the same amount of time. Next, it is important to ensure both slides are coverslipped in the came mounting media. 2) The second method is to utilize internal negatives that are already present within the patient tissue. However, this is difficult when running Fitc antibodies such as IgG, IgM, etc. We are looking into adding this addition into the software. Many customers, however have expressed that they would rather utilize the slide drawer for another patient slide, rather than running the fitc negative. Am interested in your comments. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 27 Aug 2010 19:48:15 +0100 From: "Thotakura, Anil Kumar" Subject: [Histonet] UNFIXED LIVER frozen SECTIONS To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear All, I want to make liver frozen sections but I don't want to fix the tissue. Once I make the section can I store the slides in -80 and fix them just before doing the staining?. Can you guys please suggest how to make unfixed liver frozen sections, how to store and process the tissue. Thank you very much for your help. Many Thanks, Anil Kumar. ------------------------------ Message: 6 Date: Fri, 27 Aug 2010 15:30:35 -0400 From: thomas.crowell@novartis.com Subject: [Histonet] Thomas Crowell is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 08/27/2010 and will not return until 09/07/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. ------------------------------ Message: 7 Date: Fri, 27 Aug 2010 14:30:50 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] Ventana vs Leica To: "histotech@imagesbyhopper.com" Cc: "BSullivan@shorememorial.org" , histonet , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7EC969E@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Our Techs load all the reagents and assure that the slides are labeled properly. With Ventana it is so simple to load the slides, they can go in any order so there are no mix ups. Each slide has a bar code which matches the protocol. I would caution you when looking at pricing. I have heard that if you have one slide in one of the modules on the Bond, that it needs enough fluid for 5 slides to activate the pump. That would be wasting 4/5 of the fluids needed for that module. Sounds like a-lot of waste to me. I would ask about this for sure. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histotech@imagesbyhopper.com [histotech@imagesbyhopper.com] Sent: Thursday, August 26, 2010 7:21 PM To: Mahoney,Janice A Cc: BSullivan@shorememorial.org; JayLundgren; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Unless someone corrects me (or even agrees with me!) in FL only a technologist is allowed to load the IHC machine, so no additional lean for us. :o( I would be interested to hear more about the savings though, as we are preparing to be in the market for a new IHC machine. We currently have the Benchmark XT. On Aug 26, 2010, at 10:08 AM, "Mahoney,Janice A" wrote: > We love our Ventana instruments too Jay. I don't quite believe the 40% difference in cost. I'd like to see those numbers. I know I save in tech time and the instruments are very easy to use. We have histo assistants and secretaries trained to load and unload the instruments, saving out techs to do the things only techs can do. Talk about LEAN! > A little more from a LEAN perspective, the Ultra is the only instrument out there that is "TRUE" continuous flow. As soon as there is an open spot on the instrument and the antibody is on board, I can add a slide. I don't have to wait till one of the 10 slide modules is finished. Leica is still a batch instrument, it is just smaller batches than the older IHC models. I'm not putting Leica down, it is a fine instrument but I think it is important for people to know the facts. > Jan Mahoney > Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org > Sent: Tuesday, August 24, 2010 2:05 PM > To: Jay Lundgren > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Ventana vs Leica > > Jay, > I currently use the Ventana and am very pleased with the results I get. > The only draw back is the cost to run the instrument. It can get quite > pricey. They added space on the antibody wheel but took space away from the > slide area. This has impacted our work flow greatly. We are however looking > to purchase a second one. This one will have continual through put. That > should help out with TAT. Hope this helps. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > > Jay Lundgren > l.com> To > Sent by: histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] Ventana vs Leica > 08/24/2010 02:30 > PM > > > > > > > > > I was wondering if anyone out there had experience with both the > Ventana Ultra and the Leica Bond immunostainers. I realize that most > people > have a personal preference as to brands, but I'm not looking for a > knee-jerk > opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had > actual > experience working on a daily basis with both instruments. If this is you, > could you please tell me which you preferred and why. > I'm currently working for a facility in MT which has narrowed down its > search to these two instruments. No vendors please, they've already given > their pitches. > > Thanks, > Jay A. > Lundgren M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Fri, 27 Aug 2010 14:34:43 -0500 From: Thomas Subject: RE: [Histonet] Cutting standards To: "Baez, Janet" , Rene J Buesa , histonet@lists.utsouthwestern.edu, " " Message-ID: <3s91oeduftdgdyxrqmw3awqw.1282937683422@email.android.com> Content-Type: text/plain; charset=utf-8 "Baez, Janet" wrote: >Hi Rene, > >I am interested in reading this survey. Please send me a copy. > >Thank you. >Janet Baez >Interscope Pathology Medical Group > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >Sent: Friday, August 27, 2010 8:28 AM >To: histonet@lists.utsouthwestern.edu; histotech@imagesbyhopper.com >Subject: Re: [Histonet] Cutting standards > >The averages from a survey of 325 histolabs is 50 blocks/hour to embed and 24 blocks/hour to cut. >If interested I can send you an article about this. >RenC) J. > >--- On Fri, 8/27/10, histotech@imagesbyhopper.com wrote: > > >From: histotech@imagesbyhopper.com >Subject: [Histonet] Cutting standards >To: histonet@lists.utsouthwestern.edu >Date: Friday, August 27, 2010, 9:50 AM > > >I know this question has been asked before ... Can anyone share with me what >they are actually using as a cutting/embedding standard for your techs?B For >instance, how many seconds (mins?) do you allow for embedding a block?B How >many seconds(mins?) do you allow for cutting a block? > >For simplicity here, I am looking at the "plop and drop" type specimens, ie >larger specimens that don't require specific orientation and can be placed >in a mold easily.B These types of blocks will generally have one section on >one slide.B I am trying to find out if the standard I have for my techs is >too tough or too lenient on them.B I allow 45 seconds to embed such a block >and another 45 seconds to section that same block. > >How does that fit with what you guys are all doing? > >Thanks! > >Michelle > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 27 Aug 2010 13:16:56 -0700 From: "Truscott, Tom" Subject: [Histonet] seeking part time histology job in Ann Arbor, MI To: "histonet@lists.utsouthwestern.edu" Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB4011374407D8E@CVMMBX.vetmed.wsu.edu> Content-Type: text/plain; charset="us-ascii" Hi All, My coworker, Joy, is moving to Ann Arbor in Oct. and would be interested in part time histology or pharmacy aide work while she attends classes. She has been working 7 years in fluorescent and automated IHC in a research setting. In China, she also obtained her PHD in Chinese Medicine. I can give you her contact info, if you know of a position. Thankyou, Tom Truscott ------------------------------ Message: 10 Date: Sat, 28 Aug 2010 10:33:57 -0400 From: Subject: RE: [Histonet] Ventana vs Leica To: "'Mahoney,Janice A'" Cc: BSullivan@shorememorial.org, 'histonet' , histonet-bounces@lists.utsouthwestern.edu Message-ID: <9BA66B182D6448DB8B2382CCB1DD9DF0@hopperPC> Content-Type: text/plain; charset=US-ASCII Is there anyone in FL who can confirm (or refute) my thoughts on whether or not a lab aide can load the IHC machine if a tech set up the labels for the automated staining? And if they can load it, is there any documentation to support this, just to keep my lab honest? Thanks! Michelle -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, August 27, 2010 3:31 PM To: histotech@imagesbyhopper.com Cc: BSullivan@shorememorial.org; JayLundgren; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana vs Leica Our Techs load all the reagents and assure that the slides are labeled properly. With Ventana it is so simple to load the slides, they can go in any order so there are no mix ups. Each slide has a bar code which matches the protocol. I would caution you when looking at pricing. I have heard that if you have one slide in one of the modules on the Bond, that it needs enough fluid for 5 slides to activate the pump. That would be wasting 4/5 of the fluids needed for that module. Sounds like a-lot of waste to me. I would ask about this for sure. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histotech@imagesbyhopper.com [histotech@imagesbyhopper.com] Sent: Thursday, August 26, 2010 7:21 PM To: Mahoney,Janice A Cc: BSullivan@shorememorial.org; JayLundgren; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Unless someone corrects me (or even agrees with me!) in FL only a technologist is allowed to load the IHC machine, so no additional lean for us. :o( I would be interested to hear more about the savings though, as we are preparing to be in the market for a new IHC machine. We currently have the Benchmark XT. On Aug 26, 2010, at 10:08 AM, "Mahoney,Janice A" wrote: > We love our Ventana instruments too Jay. I don't quite believe the > 40% difference in cost. I'd like to see those numbers. I know I save > in tech time and the instruments are very easy to use. We have histo > assistants and secretaries trained to load and unload the instruments, > saving out techs to do the things only techs can do. Talk about LEAN! > A little more from a LEAN perspective, the Ultra is the only > instrument out there that is "TRUE" continuous flow. As soon as there > is an open spot on the instrument and the antibody is on board, I can > add a slide. I don't have to wait till one of the 10 slide modules is > finished. Leica is still a batch instrument, it is just smaller > batches than the older IHC models. I'm not putting Leica down, it is a > fine instrument but I think it is important for people to know the > facts. Jan Mahoney Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org > Sent: Tuesday, August 24, 2010 2:05 PM > To: Jay Lundgren > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Ventana vs Leica > > Jay, > I currently use the Ventana and am very pleased with the results I > get. The only draw back is the cost to run the instrument. It can get > quite pricey. They added space on the antibody wheel but took space > away from the slide area. This has impacted our work flow greatly. We > are however looking to purchase a second one. This one will have > continual through put. That should help out with TAT. Hope this helps. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > > Jay Lundgren > l.com> To > Sent by: histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] Ventana vs Leica > 08/24/2010 02:30 > PM > > > > > > > > > I was wondering if anyone out there had experience with both the > Ventana Ultra and the Leica Bond immunostainers. I realize that most > people have a personal preference as to brands, but I'm not looking > for a knee-jerk > opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had > actual > experience working on a daily basis with both instruments. If this is you, > could you please tell me which you preferred and why. > I'm currently working for a facility in MT which has narrowed down its > search to these two instruments. No vendors please, they've already given > their pitches. > > Thanks, > Jay A. > Lundgren M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health > is faithful to the healing ministry of Jesus Christ, providing high > quality care for the body, mind and spirit of every person. > > The information contained in this communication, including > attachments, is confidential and private and intended only for the use > of the addressees. Unauthorized use, disclosure, distribution or > copying is strictly prohibited and may be unlawful. If you received > this communication in error, please inform us of the erroneous > delivery by return e-mail message from your computer. Additionally, > although all attachments have been scanned at the source for viruses, > the recipient should check any attachments for the presence of viruses > before opening. Alegent Health accepts no liability for any damage > caused by any virus transmitted by this e-mail. Thank you for your > cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3097 - Release Date: 08/27/10 06:34:00 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 39 **************************************** From azdudley <@t> hotmail.com Mon Aug 30 10:13:14 2010 From: azdudley <@t> hotmail.com (anita dudley) Date: Mon Aug 30 10:13:18 2010 Subject: [Histonet] hollande solution Message-ID: for those of you that use hollande sol for gI bx's do you wash the bxs before putting them in formalin? we started doing that thinking it would help. our pathologist are complaining the stain is not crisp on the gI's. we change and rotate our stainer often. do you run them on a short run? I am thinking that may help. is anyone using zinc fixative for them? any input would be helpful, thanks so much. anita dudley providence hosp mobile alabama From talulahgosh <@t> gmail.com Mon Aug 30 11:10:30 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Aug 30 11:10:33 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: dear rhonda, you are no longer allowed to use the internet. signed, the rest of the world -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Mon, Aug 30, 2010 at 12:41 AM, Hartz, Rhonda SktnHR < Rhonda.Hartz@saskatoonhealthregion.ca> wrote: > I had submitted a please unsubscribe email over a week ago. I am still > receiving emails. PLEASE UNSUBSCRIBE. > > Thank you. > > Rhonda Hartz > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From arvidsonkristen <@t> yahoo.com Mon Aug 30 13:26:09 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Mon Aug 30 13:27:27 2010 Subject: [Histonet] Looking for AFB control Message-ID: <475084.97352.qm@web65715.mail.ac4.yahoo.com> Hello All, I am looking for an AFB control.? I am willing to trade if I can meet your needs.? Thanks. Kristen From Stacy_McLaughlin <@t> cooley-dickinson.org Mon Aug 30 13:35:23 2010 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Mon Aug 30 13:35:28 2010 Subject: [Histonet] Post mortem safety needles Message-ID: Hello Histoland, Does anyone out there know of any blunt-ended post-mortem safety needles that can be used to suture the patient? I have checked multiple suppliers- Mopec, Thermo, etc. and everyone has pointed sharp needles. Employee safety is telling us we have to change to safety needles. What is everyone out there using for autopsies? Thanks for your help! Stacy McLaughlin HT(ASCP) Lead Histology Tech./Laboratory Safety From rparker <@t> monell.org Mon Aug 30 14:09:02 2010 From: rparker <@t> monell.org (Rocky Parker) Date: Mon Aug 30 14:09:07 2010 Subject: [Histonet] Problems with fixation of mouse testes for IHC Message-ID: Hi everyone, I'm having a difficult time getting reliable structural staining for OCT embedded samples of mouse testes. Long story short, when I use 4% PFA (4C) for 2-4h, the connective tissue between the tubules of the testis is gone or noticeably shrunken. I need to visualize the Sertoli and Leydig cells within the testes since I'm looking for differences in antibody staining (Gproteins, hormone receptors) between different knockouts. I have yet to find a method for fixation that does not require antigen retrieval and also provides great structure. Any advice is welcomed! Cheers, Rocky From sgoebel <@t> xbiotech.com Mon Aug 30 14:42:21 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Aug 30 14:50:05 2010 Subject: [Histonet] Problems with fixation of mouse testes for IHC Message-ID: <20100830124221.9e2d9aa830e8449a2412eb1e4f2f067e.719504778b.wbe@email04.secureserver.net> Try fixing in cold acetone or "PenFix" (I think you can get it th rough Fisher or the likes). Acetone is what I usually use, although i contain a require any retrieval Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg Austin, Texas 78744 < (512)386-5 -------- Original Message -------- Subject: [Histonet] Problems with fixation of mouse testes for IHC From: Rocky Parker <[1]rparker@monell Date: Mon, August 30, 2010 12:09 pm To: [2]histonet@lists.uts Hi everyone, I'm having a difficult time getting reliable structural staining for OCT embedded samples of mouse testes. Long story short, when I use 4% PFA (4C) for 2-4h, the connective tissue between the tubules of the testis is gone or noticeably shrunken. I need to visualize the Sertoli and Leydig cells within the testes since I'm looking for differences in antibody staining (Gproteins, hormone receptors) between different knockouts. I have yet to find a method for fixation that does not require antigen retrieval and also provides great structure. Any advice is welcomed! Cheers, Rocky _______________________________________________ Histonet mailing list [3]Histonet@lists.utsouth [4]http: References 1. 3D"mailto:rparker@monell.org" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From brandihiggins <@t> gmail.com Mon Aug 30 15:12:00 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Mon Aug 30 15:12:11 2010 Subject: [Histonet] accu-edge low profile microtome blades Message-ID: Hello all, We use the accu-edge low profile microtome blades (exclusively, as they work best for us). We noticed an oil/lubricant of some sort on the blades in the last box that we opened (we checked one other box and it has the same, although none previously did, or at least we didn't notice it). The oil is giving us problems with our sectioning. Has anyone else noticed this, either now or in the past? Also, does anyone have a suggestions of other blades we should use? Thanks for your input, Brandi Higgins, BS, HT(ASCP) From laurie.colbert <@t> huntingtonhospital.com Mon Aug 30 15:25:26 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Aug 30 15:25:30 2010 Subject: [Histonet] accu-edge low profile microtome blades In-Reply-To: Message-ID: <57BE698966D5C54EAE8612E8941D7683097225DF@EXCHANGE3.huntingtonhospital.com> We also use those blades and love them. We've tried others but always come back to the Accu-edge. I have always noticed a film of oil on the blades. I usually wipe the edge of the blade with a kimwipe before using it. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Monday, August 30, 2010 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] accu-edge low profile microtome blades Hello all, We use the accu-edge low profile microtome blades (exclusively, as they work best for us). We noticed an oil/lubricant of some sort on the blades in the last box that we opened (we checked one other box and it has the same, although none previously did, or at least we didn't notice it). The oil is giving us problems with our sectioning. Has anyone else noticed this, either now or in the past? Also, does anyone have a suggestions of other blades we should use? Thanks for your input, Brandi Higgins, BS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Mon Aug 30 15:54:36 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon Aug 30 15:54:47 2010 Subject: [Histonet] accu-edge low profile microtome blades In-Reply-To: References: Message-ID: It's not the oil that's giving you trouble because they have always had oil -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Monday, August 30, 2010 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] accu-edge low profile microtome blades Hello all, We use the accu-edge low profile microtome blades (exclusively, as they work best for us). We noticed an oil/lubricant of some sort on the blades in the last box that we opened (we checked one other box and it has the same, although none previously did, or at least we didn't notice it). The oil is giving us problems with our sectioning. Has anyone else noticed this, either now or in the past? Also, does anyone have a suggestions of other blades we should use? Thanks for your input, Brandi Higgins, BS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From saby_joseph_a <@t> yahoo.com Mon Aug 30 16:59:16 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Mon Aug 30 16:59:20 2010 Subject: [Histonet] (no subject) In-Reply-To: <000001cb4833$881683e0$98438ba0$@in> References: <000001cb4833$881683e0$98438ba0$@in> Message-ID: <960213.3423.qm@web114401.mail.gq1.yahoo.com> I knnow you can get vacuoles in rodent brains when processing them over the weekend with a hold station of 60-70% ethanol.? Other than that I cannot say. Joe Saby, BA HT ________________________________ From: Pathology To: histonet@lists.utsouthwestern.edu Sent: Mon, August 30, 2010 7:07:22 AM Subject: [Histonet] (no subject) Hi , How to avoid vacuoles in the sections of brain and testes of rats. Whether it is the problem of fixing, processing or staining. Thanks & Regards, S.Naveen Babu Technician - Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rhonda.Hartz <@t> saskatoonhealthregion.ca Mon Aug 30 17:52:11 2010 From: Rhonda.Hartz <@t> saskatoonhealthregion.ca (Hartz, Rhonda SktnHR) Date: Mon Aug 30 17:52:19 2010 Subject: [Histonet] (no subject) Message-ID: To all of you who helped me out here regarding unsubscribing, I truly appreciate it and apologize. Did not realize how it sounded until you sent it back to me. I had just assumed that when I received emails that said please unsubscribe from others that it was a message that they sent. Thank you for all of your directions. I will follow them. Rhonda Hartz From graceofgod011978 <@t> hotmail.com Mon Aug 30 22:40:00 2010 From: graceofgod011978 <@t> hotmail.com (Emmanuel O) Date: Mon Aug 30 22:40:04 2010 Subject: [Histonet] Looking for new IHC position In-Reply-To: References: Message-ID: Hi, I am a certified HISTOLOGIST and looking for a new position as IHC SPECIALIST. I am open to any shift and relocation. I am HTL(ASCP). I also have QIHC(ASCP). I have many years of experience. I am looking for position as either Histology Supervisor, Lead TECH or as IHC SPECIALIST. Emmanuel. Emmanuel. From pathology01 <@t> rccltd.in Mon Aug 30 23:17:58 2010 From: pathology01 <@t> rccltd.in (Pathology) Date: Mon Aug 30 23:17:21 2010 Subject: [Histonet] coated slides Message-ID: <001101cb48c3$802a0090$807e01b0$@in> Hi, What is the best option for coating microscope slides for routine histopathology. Please mention the best and easy method of coating slides manually. Thanks & Regards, S.Naveen Babu Technician - Pathology ? ? Disclaimer Information transmitted by this E-MAIL is proprietary to RCC Laboratories India Pvt. Ltd. and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Looking for new position (Emmanuel O) 2. RE: Rapid liver core biopsy processing (Tony Henwood) 3. (no subject) (Hartz, Rhonda SktnHR) 4. Re: (no subject) (Lee & Peggy Wenk) 5. (no subject) (Pathology) 6. RE: Histonet Digest, Vol 81, Issue 39 (Pathology) 7. hollande solution (anita dudley) 8. Re: (no subject) (Emily Sours) ---------------------------------------------------------------------- Message: 1 Date: Sun, 29 Aug 2010 19:52:42 -0500 From: Emmanuel O Subject: [Histonet] Looking for new position To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I am a certified HISTOLOGIST and looking for a new position as either a bech histotech. I am open to any shift and relocation.. Emmanuel. ------------------------------ Message: 2 Date: Mon, 30 Aug 2010 12:07:00 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Rapid liver core biopsy processing To: "Garth Fraga" , Message-ID: Content-Type: text/plain; charset="us-ascii" Garth, We have two methods available: The first is a microwave method using the Milestone Mega TT. It uses 10% NBF (25min at 35oC followed by 25min at 50oC), followed by an ethanol rinse (2-3min room temp), 7min at 69oC in isopropanol and finally 8 min at 79oC in wax. The second uses our routine processor, 1hr in NBF at 40oC, followed by ethanol 10min x 4, xylene 10min x3, wax 10min x3. I have found it important to extend the NBF fixation step as much as I can - gives better results. Also these methods apply to core biopsies or very thin slices (<1mm) Hope this helps Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garth Fraga Sent: Friday, 27 August 2010 3:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rapid liver core biopsy processing Dear histonetters, We do about a hundred liver transplants/year at our hospital, and the hepatologists do lots of liver core biopsies to rule out rejection. They want a same-day diagnosis on these, so historically they have been rush processed on a two-hour cycle (VIP machine). They are brought over directly from the liver biopsy suite immediately after biopsy, so they get very little fixation in the specimen container prior to going on the processor. Recently we had a couple of sub-par cases and have moved to a four-hour processing cycle. Do any of you have any experience dealing with rush-processed liver cores in transplant patients? What sort of a processing schedule do you recommend? Anyone handling them like frozen sections? Thanks, Garth Fraga (pathologist) University of Kansas Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **************************************************************************** ***** ------------------------------ Message: 3 Date: Sun, 29 Aug 2010 22:41:29 -0600 From: "Hartz, Rhonda SktnHR" Subject: [Histonet] (no subject) To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I had submitted a please unsubscribe email over a week ago. I am still receiving emails. PLEASE UNSUBSCRIBE. Thank you. Rhonda Hartz ------------------------------ Message: 4 Date: Mon, 30 Aug 2010 05:28:04 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] (no subject) To: "Hartz, Rhonda SktnHR" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Look at the bottom of any email from Histonet. The 2nd link says "lists.utsouthwestern" - click on it. At the bottom of the page is directions on how to unsubscribe. Follow the directions. Peggy Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Hartz, Rhonda SktnHR" Sent: Monday, August 30, 2010 12:41 AM To: Subject: [Histonet] (no subject) > I had submitted a please unsubscribe email over a week ago. I am still > receiving emails. PLEASE UNSUBSCRIBE. > > Thank you. > > Rhonda Hartz > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 30 Aug 2010 16:37:22 +0530 From: "Pathology" Subject: [Histonet] (no subject) To: Message-ID: <000001cb4833$881683e0$98438ba0$@in> Content-Type: text/plain; charset="us-ascii" Hi , How to avoid vacuoles in the sections of brain and testes of rats. Whether it is the problem of fixing, processing or staining. Thanks & Regards, S.Naveen Babu Technician - Pathology ------------------------------ Message: 6 Date: Mon, 30 Aug 2010 16:43:27 +0530 From: "Pathology" Subject: [Histonet] RE: Histonet Digest, Vol 81, Issue 39 To: Message-ID: <000501cb4834$62d8b660$288a2320$@in> Content-Type: text/plain; charset="iso-8859-1" Hi Reni, I am interested in reading this survey. Please send me a copy. Thanks & Regards, S.Naveen Babu Technician - Pathology Genome Valley, Shameerpet, Hyderabad - 500 078. A.P. India. Tel.: +91 40 2348 0422 Fax: +91 40 2348 0420 www.rccltd.in Disclaimer Information transmitted by this E-MAIL is proprietary to RCC Laboratories India Pvt. Ltd. and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: FITC on Ventana Ultra (Mark Tarango) 2. Re: Specimen Retention (BSullivan@shorememorial.org) 3. Re: Re: Rapid liver core biopsy processing (Deanna Rhoads) 4. FW: [Histonet] FITC on Ventana Ultra (Kasprowicz, Emily E) 5. UNFIXED LIVER frozen SECTIONS (Thotakura, Anil Kumar) 6. Thomas Crowell is out of the office. (thomas.crowell@novartis.com) 7. RE: Ventana vs Leica (Mahoney,Janice A) 8. RE: Cutting standards (Thomas) 9. seeking part time histology job in Ann Arbor, MI (Truscott, Tom) 10. RE: Ventana vs Leica (histotech@imagesbyhopper.com) ---------------------------------------------------------------------- Message: 1 Date: Fri, 27 Aug 2010 10:18:07 -0700 From: Mark Tarango Subject: Re: [Histonet] FITC on Ventana Ultra To: Nita Searcy Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Nita, I agree that buffer should be used as the negative control reagent. If you have a seperate slide that is cut and put into buffer, just coverslip it with the same mounting media and you have a negative control. All the instrument is doing is putting on the antibody and then rinsing it off. So your negative control is a slide that didn't get the antibody (just buffer). Makes sense to me. If you get ventana to add a negative control to their software it would do just the same thing as coverslipping from buffer, but you'd be paying ventana for it. Mark On Fri, Aug 27, 2010 at 7:30 AM, Nita Searcy wrote: > Any users out there that have had A CAP inspector question negative control > on the instrument? In regard to CAP question ANP.21850 in which the notes > state, "A negative reagent control in which the patient tissue is processed > in an identical manner to the test specimen but with the primary antibody > omitted must be performed for each patient test specimen?" > > Below is the response from Ventana regarding "negatives" I am curios if CAP > accepts these methods?? > > In regards to the question below about running a negative control fitc, > there are two ways to accomplish this. > > > > 1) There is not currently a place in the protocol to select a fitc > negative. We can still be compliant by ensuring the negative slide is > treated the same as the patient. The only reagent the patient slide is > exposed to, besides the fitc antibody, is reaction buffer. Running a > negative can be accomplished by applying reaction buffer to the slide and > letting it incubate for the same amount of time. Next, it is important to > ensure both slides are coverslipped in the came mounting media. > 2) The second method is to utilize internal negatives that are already > present within the patient tissue. However, this is difficult when running > Fitc antibodies such as IgG, IgM, etc. > We are looking into adding this addition into the software. Many customers, > however have expressed that they would rather utilize the slide drawer for > another patient slide, rather than running the fitc negative. > > Am interested in your comments. > Thanks > > > > > > Nita Searcy, HT/HTL (ASCP) > Scott and White Hospital > Division Manager, Anatomic Pathology > 2401 S. 31st. Street > 254-724-2438 > Temple, Texas, 76502 > nsearcy@swmail.sw.org > > > 254-724-2438 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 2 Date: Fri, 27 Aug 2010 13:18:43 -0400 From: BSullivan@shorememorial.org Subject: Re: [Histonet] Specimen Retention To: "Laurie Colbert" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII We keep all tissue sent to us for the required time . This includes hardware and foreign bodies etc., etc. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Laurie Colbert" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Specimen Retention 08/27/2010 11:56 AM CAP states that all gross specimens and wet tissue be retained until at least 2 weeks after the final reports are reported out. Do others follow these guidelines for hardware, foreign bodies, etc, or would you possibly return them to the patient sooner than the 2 weeks? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 27 Aug 2010 10:28:04 -0700 (PDT) From: Deanna Rhoads Subject: Re: [Histonet] Re: Rapid liver core biopsy processing To: Robert Richmond , histonet@lists.utsouthwestern.edu Message-ID: <671126.93121.qm@web84305.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I worked at the University of Pittsburgh Medical Center and we did the liver biopsies on a stat, 2 hour run, on the Leica Peloris. We got great results and quick diagnosis. Deanna Rhoads HT (ASCP) Pittsburgh, PA ________________________________ From: Robert Richmond To: histonet@lists.utsouthwestern.edu Sent: Fri, August 27, 2010 12:03:18 PM Subject: [Histonet] Re: Rapid liver core biopsy processing Garth Fraga, a pathologist at the University of Kansas Medical Center asks: >>We do about a hundred liver transplants/year at our hospital, and the >>hepatologists do lots of liver core biopsies to rule out rejection. They want a >>same-day diagnosis on these, so historically they have been rush processed on a >>two-hour cycle (VIP machine). They are brought over directly from the liver >>biopsy suite immediately after biopsy, so they get very little fixation in the >>specimen container prior to going on the processor. Recently we had a couple of >>sub-par cases and have moved to a four-hour processing cycle. Do any of you have >>any experience dealing with rush-processed liver cores in transplant patients? >>What sort of a processing schedule do you recommend? Anyone handling them like >>frozen sections?<< I would address this question to the pathologists at the University of Pittsburgh, since this is the foremost liver transplant service possibly in the world. I've consulted their Web site about liver transplants - quite a while ago - and it was quite helpful. Garth, if you get a reply, could you post it on Histonet for us all to see? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 27 Aug 2010 13:15:40 -0500 From: "Kasprowicz, Emily E" Subject: FW: [Histonet] FITC on Ventana Ultra To: "histonet@lists.utsouthwestern.edu" Message-ID: <66FFB482DE2B624DA30B826C179538214E3AAF2645@MCD-MBX-3.HCMC.CO.HENNEPIN.MN.US > Content-Type: text/plain; charset="us-ascii" Emily Kasprowicz, HT (ASCP) Histology Lab Hennepin County Medical Center 701 Park Avenue Minneapolis, MN 55415 612-873-3079 ________________________________________ From: Kasprowicz, Emily E Sent: Friday, August 27, 2010 1:13 PM To: Morken, Tim Subject: RE: [Histonet] FITC on Ventana Ultra We have a Benchmark XT and LT, and this has been somewhat of a nuisance, but not as much as the procedure that you have described. We, too, have to trick the system. What we have come up with (however, we have not been inspected by CAP since implementing this, so I don't know if this will be acceptable to the inspector) is to make a "titer" protocol for each of our antibodies. The protocol is exactly the same as our normal run, but we have to manually apply the antibody to the correct slide (we label our test slides with the lot numbers and "Old Lot" and "New Lot") at the time of antibody incubation. This was the least complicated way we could come up with to do the "side by side" testing required by CAP, and it seems to work pretty well. Our problem is is that we have to wait for a time when one of the machines is totally free so we can do the titer run. This is, of course, not very often, so we have to try to do lot comparisons as far in advance as possible. If we had the Ventana Ultra, it would be a little easier as we wouldn't have to hold up the entire machine for a Titer Run. We could just run the slides as needed. If anyone else has a better way to do lot checks on the Ventana Benchmarks, I'd be interested in hearing your procedure as well. Thanks, Emily Kasprowicz, HT (ASCP) Histology Lab Hennepin County Medical Center 701 Park Avenue Minneapolis, MN 55415 612-873-3079 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim [Timothy.Morken@ucsfmedctr.org] Sent: Friday, August 27, 2010 11:52 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FITC on Ventana Ultra Part of the Ventana response was "Many customers, however have expressed that they would rather utilize the slide drawer for another patient slide, rather than running the fitc negative." I hope the customers come to understand that the negative control is necessary, not just an option. Also, it has come to light that it is difficult to run lot comparison tests on some automated instruments as CAP is now requiring. From Ventana the information I have gotten is that the customer has to "trick" the system to be able to test two lots in one run. Two lots of the same antibody can be used on one run, but the system will use the oldest lot first, then switch to the new lot when the old lot runs out. So to do a lot comparison the customer must arrange to use the last test from the old lot on one slide and then assume the system will switch to the new lot for the other slide. Hopefully it works. I don't use Ventana but am interested because we are looking at the system, plus I am giving a validation workshop at NSH and one of the most common questions now is how to do a lot comparison test on an automated system. I welcome any other information on that process on any system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, August 27, 2010 7:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FITC on Ventana Ultra Any users out there that have had A CAP inspector question negative control on the instrument? In regard to CAP question ANP.21850 in which the notes state, "A negative reagent control in which the patient tissue is processed in an identical manner to the test specimen but with the primary antibody omitted must be performed for each patient test specimen?" Below is the response from Ventana regarding "negatives" I am curios if CAP accepts these methods?? In regards to the question below about running a negative control fitc, there are two ways to accomplish this. 1) There is not currently a place in the protocol to select a fitc negative. We can still be compliant by ensuring the negative slide is treated the same as the patient. The only reagent the patient slide is exposed to, besides the fitc antibody, is reaction buffer. Running a negative can be accomplished by applying reaction buffer to the slide and letting it incubate for the same amount of time. Next, it is important to ensure both slides are coverslipped in the came mounting media. 2) The second method is to utilize internal negatives that are already present within the patient tissue. However, this is difficult when running Fitc antibodies such as IgG, IgM, etc. We are looking into adding this addition into the software. Many customers, however have expressed that they would rather utilize the slide drawer for another patient slide, rather than running the fitc negative. Am interested in your comments. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 27 Aug 2010 19:48:15 +0100 From: "Thotakura, Anil Kumar" Subject: [Histonet] UNFIXED LIVER frozen SECTIONS To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear All, I want to make liver frozen sections but I don't want to fix the tissue. Once I make the section can I store the slides in -80 and fix them just before doing the staining?. Can you guys please suggest how to make unfixed liver frozen sections, how to store and process the tissue. Thank you very much for your help. Many Thanks, Anil Kumar. ------------------------------ Message: 6 Date: Fri, 27 Aug 2010 15:30:35 -0400 From: thomas.crowell@novartis.com Subject: [Histonet] Thomas Crowell is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 08/27/2010 and will not return until 09/07/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. ------------------------------ Message: 7 Date: Fri, 27 Aug 2010 14:30:50 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] Ventana vs Leica To: "histotech@imagesbyhopper.com" Cc: "BSullivan@shorememorial.org" , histonet , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7EC969E@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Our Techs load all the reagents and assure that the slides are labeled properly. With Ventana it is so simple to load the slides, they can go in any order so there are no mix ups. Each slide has a bar code which matches the protocol. I would caution you when looking at pricing. I have heard that if you have one slide in one of the modules on the Bond, that it needs enough fluid for 5 slides to activate the pump. That would be wasting 4/5 of the fluids needed for that module. Sounds like a-lot of waste to me. I would ask about this for sure. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histotech@imagesbyhopper.com [histotech@imagesbyhopper.com] Sent: Thursday, August 26, 2010 7:21 PM To: Mahoney,Janice A Cc: BSullivan@shorememorial.org; JayLundgren; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Unless someone corrects me (or even agrees with me!) in FL only a technologist is allowed to load the IHC machine, so no additional lean for us. :o( I would be interested to hear more about the savings though, as we are preparing to be in the market for a new IHC machine. We currently have the Benchmark XT. On Aug 26, 2010, at 10:08 AM, "Mahoney,Janice A" wrote: > We love our Ventana instruments too Jay. I don't quite believe the 40% difference in cost. I'd like to see those numbers. I know I save in tech time and the instruments are very easy to use. We have histo assistants and secretaries trained to load and unload the instruments, saving out techs to do the things only techs can do. Talk about LEAN! > A little more from a LEAN perspective, the Ultra is the only instrument out there that is "TRUE" continuous flow. As soon as there is an open spot on the instrument and the antibody is on board, I can add a slide. I don't have to wait till one of the 10 slide modules is finished. Leica is still a batch instrument, it is just smaller batches than the older IHC models. I'm not putting Leica down, it is a fine instrument but I think it is important for people to know the facts. > Jan Mahoney > Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org > Sent: Tuesday, August 24, 2010 2:05 PM > To: Jay Lundgren > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Ventana vs Leica > > Jay, > I currently use the Ventana and am very pleased with the results I get. > The only draw back is the cost to run the instrument. It can get quite > pricey. They added space on the antibody wheel but took space away from the > slide area. This has impacted our work flow greatly. We are however looking > to purchase a second one. This one will have continual through put. That > should help out with TAT. Hope this helps. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > > Jay Lundgren > l.com> To > Sent by: histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] Ventana vs Leica > 08/24/2010 02:30 > PM > > > > > > > > > I was wondering if anyone out there had experience with both the > Ventana Ultra and the Leica Bond immunostainers. I realize that most > people > have a personal preference as to brands, but I'm not looking for a > knee-jerk > opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had > actual > experience working on a daily basis with both instruments. If this is you, > could you please tell me which you preferred and why. > I'm currently working for a facility in MT which has narrowed down its > search to these two instruments. No vendors please, they've already given > their pitches. > > Thanks, > Jay A. > Lundgren M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Fri, 27 Aug 2010 14:34:43 -0500 From: Thomas Subject: RE: [Histonet] Cutting standards To: "Baez, Janet" , Rene J Buesa , histonet@lists.utsouthwestern.edu, " " Message-ID: <3s91oeduftdgdyxrqmw3awqw.1282937683422@email.android.com> Content-Type: text/plain; charset=utf-8 "Baez, Janet" wrote: >Hi Rene, > >I am interested in reading this survey. Please send me a copy. > >Thank you. >Janet Baez >Interscope Pathology Medical Group > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >Sent: Friday, August 27, 2010 8:28 AM >To: histonet@lists.utsouthwestern.edu; histotech@imagesbyhopper.com >Subject: Re: [Histonet] Cutting standards > >The averages from a survey of 325 histolabs is 50 blocks/hour to embed and 24 blocks/hour to cut. >If interested I can send you an article about this. >RenC) J. > >--- On Fri, 8/27/10, histotech@imagesbyhopper.com wrote: > > >From: histotech@imagesbyhopper.com >Subject: [Histonet] Cutting standards >To: histonet@lists.utsouthwestern.edu >Date: Friday, August 27, 2010, 9:50 AM > > >I know this question has been asked before ... Can anyone share with me what >they are actually using as a cutting/embedding standard for your techs?B For >instance, how many seconds (mins?) do you allow for embedding a block?B How >many seconds(mins?) do you allow for cutting a block? > >For simplicity here, I am looking at the "plop and drop" type specimens, ie >larger specimens that don't require specific orientation and can be placed >in a mold easily.B These types of blocks will generally have one section on >one slide.B I am trying to find out if the standard I have for my techs is >too tough or too lenient on them.B I allow 45 seconds to embed such a block >and another 45 seconds to section that same block. > >How does that fit with what you guys are all doing? > >Thanks! > >Michelle > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 27 Aug 2010 13:16:56 -0700 From: "Truscott, Tom" Subject: [Histonet] seeking part time histology job in Ann Arbor, MI To: "histonet@lists.utsouthwestern.edu" Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB4011374407D8E@CVMMBX.vetmed.wsu.edu> Content-Type: text/plain; charset="us-ascii" Hi All, My coworker, Joy, is moving to Ann Arbor in Oct. and would be interested in part time histology or pharmacy aide work while she attends classes. She has been working 7 years in fluorescent and automated IHC in a research setting. In China, she also obtained her PHD in Chinese Medicine. I can give you her contact info, if you know of a position. Thankyou, Tom Truscott ------------------------------ Message: 10 Date: Sat, 28 Aug 2010 10:33:57 -0400 From: Subject: RE: [Histonet] Ventana vs Leica To: "'Mahoney,Janice A'" Cc: BSullivan@shorememorial.org, 'histonet' , histonet-bounces@lists.utsouthwestern.edu Message-ID: <9BA66B182D6448DB8B2382CCB1DD9DF0@hopperPC> Content-Type: text/plain; charset=US-ASCII Is there anyone in FL who can confirm (or refute) my thoughts on whether or not a lab aide can load the IHC machine if a tech set up the labels for the automated staining? And if they can load it, is there any documentation to support this, just to keep my lab honest? Thanks! Michelle -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, August 27, 2010 3:31 PM To: histotech@imagesbyhopper.com Cc: BSullivan@shorememorial.org; JayLundgren; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana vs Leica Our Techs load all the reagents and assure that the slides are labeled properly. With Ventana it is so simple to load the slides, they can go in any order so there are no mix ups. Each slide has a bar code which matches the protocol. I would caution you when looking at pricing. I have heard that if you have one slide in one of the modules on the Bond, that it needs enough fluid for 5 slides to activate the pump. That would be wasting 4/5 of the fluids needed for that module. Sounds like a-lot of waste to me. I would ask about this for sure. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histotech@imagesbyhopper.com [histotech@imagesbyhopper.com] Sent: Thursday, August 26, 2010 7:21 PM To: Mahoney,Janice A Cc: BSullivan@shorememorial.org; JayLundgren; histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Unless someone corrects me (or even agrees with me!) in FL only a technologist is allowed to load the IHC machine, so no additional lean for us. :o( I would be interested to hear more about the savings though, as we are preparing to be in the market for a new IHC machine. We currently have the Benchmark XT. On Aug 26, 2010, at 10:08 AM, "Mahoney,Janice A" wrote: > We love our Ventana instruments too Jay. I don't quite believe the > 40% difference in cost. I'd like to see those numbers. I know I save > in tech time and the instruments are very easy to use. We have histo > assistants and secretaries trained to load and unload the instruments, > saving out techs to do the things only techs can do. Talk about LEAN! > A little more from a LEAN perspective, the Ultra is the only > instrument out there that is "TRUE" continuous flow. As soon as there > is an open spot on the instrument and the antibody is on board, I can > add a slide. I don't have to wait till one of the 10 slide modules is > finished. Leica is still a batch instrument, it is just smaller > batches than the older IHC models. I'm not putting Leica down, it is a > fine instrument but I think it is important for people to know the > facts. Jan Mahoney Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org > Sent: Tuesday, August 24, 2010 2:05 PM > To: Jay Lundgren > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Ventana vs Leica > > Jay, > I currently use the Ventana and am very pleased with the results I > get. The only draw back is the cost to run the instrument. It can get > quite pricey. They added space on the antibody wheel but took space > away from the slide area. This has impacted our work flow greatly. We > are however looking to purchase a second one. This one will have > continual through put. That should help out with TAT. Hope this helps. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > > Jay Lundgren > l.com> To > Sent by: histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] Ventana vs Leica > 08/24/2010 02:30 > PM > > > > > > > > > I was wondering if anyone out there had experience with both the > Ventana Ultra and the Leica Bond immunostainers. I realize that most > people have a personal preference as to brands, but I'm not looking > for a knee-jerk > opinion (LEICA RULZ!!!!11 or VENTANA FTW!!), just someone who has had > actual > experience working on a daily basis with both instruments. If this is you, > could you please tell me which you preferred and why. > I'm currently working for a facility in MT which has narrowed down its > search to these two instruments. No vendors please, they've already given > their pitches. > > Thanks, > Jay A. > Lundgren M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health > is faithful to the healing ministry of Jesus Christ, providing high > quality care for the body, mind and spirit of every person. > > The information contained in this communication, including > attachments, is confidential and private and intended only for the use > of the addressees. Unauthorized use, disclosure, distribution or > copying is strictly prohibited and may be unlawful. If you received > this communication in error, please inform us of the erroneous > delivery by return e-mail message from your computer. Additionally, > although all attachments have been scanned at the source for viruses, > the recipient should check any attachments for the presence of viruses > before opening. Alegent Health accepts no liability for any damage > caused by any virus transmitted by this e-mail. Thank you for your > cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3097 - Release Date: 08/27/10 06:34:00 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 39 **************************************** ------------------------------ Message: 7 Date: Mon, 30 Aug 2010 10:13:14 -0500 From: anita dudley Subject: [Histonet] hollande solution To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" for those of you that use hollande sol for gI bx's do you wash the bxs before putting them in formalin? we started doing that thinking it would help. our pathologist are complaining the stain is not crisp on the gI's. we change and rotate our stainer often. do you run them on a short run? I am thinking that may help. is anyone using zinc fixative for them? any input would be helpful, thanks so much. anita dudley providence hosp mobile alabama ------------------------------ Message: 8 Date: Mon, 30 Aug 2010 12:10:30 -0400 From: Emily Sours Subject: Re: [Histonet] (no subject) To: "Hartz, Rhonda SktnHR" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 dear rhonda, you are no longer allowed to use the internet. signed, the rest of the world -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Mon, Aug 30, 2010 at 12:41 AM, Hartz, Rhonda SktnHR < Rhonda.Hartz@saskatoonhealthregion.ca> wrote: > I had submitted a please unsubscribe email over a week ago. I am still > receiving emails. PLEASE UNSUBSCRIBE. > > Thank you. > > Rhonda Hartz > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 41 **************************************** From ccrowder <@t> vetmed.lsu.edu Mon Aug 30 23:19:36 2010 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Mon Aug 30 23:24:23 2010 Subject: [Histonet] Vacuoles Message-ID: Nareen - Vacuoles in brain and rat testes are often caused by the block being too cold and dry. Try facing off the block and then placing on melting ice or cold water. This will rehydrate the block and raise the temperature. Also, cut slowly, particularly the brain. Vacuoles seen to appear more when the block is cut quickly. Cheryl From histonet.nospam <@t> vneubert.com Tue Aug 31 03:25:36 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Tue Aug 31 03:25:47 2010 Subject: [Histonet] coated slides In-Reply-To: <001101cb48c3$802a0090$807e01b0$@in> References: <001101cb48c3$802a0090$807e01b0$@in> Message-ID: <4C7CBC80.20603@vneubert.com> Hi, you could buy "Super Frost Plus" slides. If you want to coat slides by yourself, you could buy "poly-L lysine" 0.1mg/mL. Put a drop of about 5?L on the left upper corner of a slide, then put two of those slides together (surface on surface), move them around until the lysine is spread all over the slides, then seperate them by just pulling without letting any air between the slides. Easy :) From MITCHELLJA <@t> email.chop.edu Tue Aug 31 08:43:16 2010 From: MITCHELLJA <@t> email.chop.edu (Mitchell, Janice A) Date: Tue Aug 31 08:43:24 2010 Subject: [Histonet] LIS systems Message-ID: Good Morning, We are looking for new LIS system. Right now there are two options, a system for all of Clinical Labs & AP or a new system for just AP. Right now we use Meditech. Any advice on which systems work best for AP. I know each system will have some flaw since nothing is perfect but, anything has got to be better than Meditech. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Assistant Histology Supervisor Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) From Janice.Mahoney <@t> alegent.org Tue Aug 31 08:46:16 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Aug 31 08:47:07 2010 Subject: [Histonet] RE: LIS systems In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E23@EXCHMBC2.ad.ah.local> I love Cerner Millennium. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, August 31, 2010 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS systems Good Morning, We are looking for new LIS system. Right now there are two options, a system for all of Clinical Labs & AP or a new system for just AP. Right now we use Meditech. Any advice on which systems work best for AP. I know each system will have some flaw since nothing is perfect but, anything has got to be better than Meditech. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Assistant Histology Supervisor Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From SHayes <@t> bloomingtonhospital.org Tue Aug 31 08:56:37 2010 From: SHayes <@t> bloomingtonhospital.org (Hayes, Sherry) Date: Tue Aug 31 08:56:46 2010 Subject: [Histonet] RE: LIS systems In-Reply-To: References: Message-ID: <8A307C4C42FC17478A648E933A64AAA9976EB4CD7F@XCHMBCL1.neutzone.bloomhealth.org> We went from Cerner Classic to PowerPath and HLAB. We love PowerPath. HLAB has pluses and minuses. Sherry S. Hayes Laboratory Systems Support Coordinator Bloomington Hospital 601 West Second Street PO Box 1149 Bloomington, IN 47402 t812.353.5606 f812.353.5584 p812.334.6337 shayes@bloomingtonhospital.org bloomingtonhospital.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, August 31, 2010 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS systems Good Morning, We are looking for new LIS system. Right now there are two options, a system for all of Clinical Labs & AP or a new system for just AP. Right now we use Meditech. Any advice on which systems work best for AP. I know each system will have some flaw since nothing is perfect but, anything has got to be better than Meditech. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Assistant Histology Supervisor Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If you are not the intended recipient, you may NOT use, disclose, copy or disseminate this information. Please contact the sender by reply e-mail immediately and destroy all copies of the original message including all attachments. Your cooperation is greatly appreciated. Bloomington Hospital P.O. Box 1149 Bloomington, IN 47402 From chak_bou <@t> yahoo.com Tue Aug 31 09:22:41 2010 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Tue Aug 31 09:22:45 2010 Subject: [Histonet] Warthin-Starry Stain Message-ID: <597018.48678.qm@web58101.mail.re3.yahoo.com> Hi Histonet, Does anyone out there uses Warthin-Starry Stain, If so, Can you help me with troubleshooting, my slides get overstained even though i respect the developing time that is in the procedure which is 3.5min. thank you in advance and your help will be very much appreciated! Chakib HTL(ASCP) MIT From lblazek <@t> digestivespecialists.com Tue Aug 31 09:23:38 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Aug 31 09:25:06 2010 Subject: [Histonet] RE: LIS systems In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E390EAF72C798@IBMB7Exchange.digestivespecialists.com> I've never used Meditech so I don't know what the down side is to that system. I have used PowerPath and it was a great system but if you want a system that has most of what PowerPath has but not the enormous cost you may want to look at Pathlogix. http://www.pathlogix.com/ I use it now and like it. It does have some glitch things but they are not major and there is a simple workaround for them. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, August 31, 2010 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS systems Good Morning, We are looking for new LIS system. Right now there are two options, a system for all of Clinical Labs & AP or a new system for just AP. Right now we use Meditech. Any advice on which systems work best for AP. I know each system will have some flaw since nothing is perfect but, anything has got to be better than Meditech. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Assistant Histology Supervisor Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Tue Aug 31 09:30:08 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Aug 31 09:30:14 2010 Subject: [Histonet] RE: LIS systems In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EAF72C798@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E390EAF72C798@IBMB7Exchange.digestivespecialists.com> Message-ID: <4C7D11F0.1070008@pathology.washington.edu> We have used PowerPath since 1999 and have made so many custom changes that we probably can't ever leave. If I were looking today I would consider http://www.pathview.com/. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 8/31/2010 7:23 AM, Blazek, Linda wrote: > I've never used Meditech so I don't know what the down side is to that system. I have used PowerPath and it was a great system but if you want a system that has most of what PowerPath has but not the enormous cost you may want to look at Pathlogix. http://www.pathlogix.com/ > I use it now and like it. It does have some glitch things but they are not major and there is a simple workaround for them. > > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Digestive Specialists, Inc > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A > Sent: Tuesday, August 31, 2010 9:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] LIS systems > > Good Morning, > We are looking for new LIS system. Right now there are two options, a system for all of Clinical Labs& AP or a new system for just AP. Right now we use Meditech. Any advice on which systems work best for AP. I know each system will have some flaw since nothing is perfect but, anything has got to be better than Meditech. > > Thanks, Janice > > Janice A. Mitchell, BS, HT(ASCP) > Assistant Histology Supervisor > Children's Hospital of Philadelphia > Anatomic Pathology and Laboratory Medicine > 324 S. 34th Street > Philadelphia, Pa 19104-4399 > 215-590-1738(lab) > 267-426-7754(office) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Tue Aug 31 09:31:18 2010 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Tue Aug 31 09:32:00 2010 Subject: [Histonet] Warthin-Starry Stain References: <597018.48678.qm@web58101.mail.re3.yahoo.com> Message-ID: It has been my experience that if I use charged slides I get overstaining. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Chakib Boussahmain Sent: Tue 8/31/2010 9:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Warthin-Starry Stain Hi Histonet, Does anyone out there uses Warthin-Starry Stain, If so, Can you help me with troubleshooting, my slides get overstained even though i respect the developing time that is in the procedure which is 3.5min. thank you in advance and your help will be very much appreciated! Chakib HTL(ASCP) MIT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Tue Aug 31 09:48:11 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Aug 31 09:48:18 2010 Subject: [Histonet] Warthin-Starry Stain Message-ID: <20100831074811.9e2d9aa830e8449a2412eb1e4f2f067e.49cb93bb3a.wbe@email04.secureserver.net> I have always used the microwave method so...try to leaving the s lides in the silver nitrate less time. Remember you want the solution hydroquinone. aren't over developing color of a wood grain do stage, stop the developing. the times by looking at the slide, y all of them all the time during developing.& alot of trouble with the WS stain when i first star too, and this was my best way of figuring it out...check as y staining...no more overstained slides. =) Hope this he Sarah Goebel, B.A., HT (ASCP) Histotechnician < XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 1 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] Warthin-Starry Stain From: Chakib Boussahmain <[1]chak_bou Date: Tue, August 31, 2010 7:22 am To: [2]histonet@lists.uts Hi Histonet, Does anyone out there uses Warthin-Starry Stain, If so, Can you help me wit respect the deve 3.5min. thank you in advance and your help will be very much appreciated! Chakib HTL(ASCP) MIT _______________________________________________ Histonet mailing list [3]Histonet@lists.utsouth [4]http: References 1. 3D"mailto:chak_bou@yahoo.com" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From JEllin <@t> yumaregional.org Tue Aug 31 10:01:39 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Aug 31 10:01:07 2010 Subject: [Histonet] RE: LIS systems In-Reply-To: <4C7D11F0.1070008@pathology.washington.edu> References: <5A2BD13465E061429D6455C8D6B40E390EAF72C798@IBMB7Exchange.digestivespecialists.com> <4C7D11F0.1070008@pathology.washington.edu> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C6686@EXCHANGECLUSTER.yumaregional.local> We have used PowerPath here and it works great for us. It also depends on what you want to accomplish with the LIS. This is very important to look at this aspect and define what your goals are, costs, and future functionality. The smaller system will be able to customize well, while the larger systems are pretty much set in there ways. If I had to pick top 3 it would be PowerPath, Pathview, and Pathlogic. The larger systems have issues and unless if you are part of an enterprise system that requires you to be one vendor, I would keep my mind open. But ask these systems about workflow and how they are going to meet the role of the new future of QA and QC within the AP Laboratory. They are so pathologist centric and they forget about the other aspects of the AP lab to include cytology, molecular, grossing, etc. Jesus Ellin HT/PA ASCP BSBE Anatomic Pathology Supervisor Department of Pathology Information Systems Yuma Regional Medical Center Yuma,AZ jellin@yumaregional.org 928-336-1743 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Tuesday, August 31, 2010 7:30 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: LIS systems We have used PowerPath since 1999 and have made so many custom changes that we probably can't ever leave. If I were looking today I would consider http://www.pathview.com/. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 8/31/2010 7:23 AM, Blazek, Linda wrote: > I've never used Meditech so I don't know what the down side is to that system. I have used PowerPath and it was a great system but if you want a system that has most of what PowerPath has but not the enormous cost you may want to look at Pathlogix. http://www.pathlogix.com/ > I use it now and like it. It does have some glitch things but they are not major and there is a simple workaround for them. > > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Digestive Specialists, Inc > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A > Sent: Tuesday, August 31, 2010 9:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] LIS systems > > Good Morning, > We are looking for new LIS system. Right now there are two options, a system for all of Clinical Labs& AP or a new system for just AP. Right now we use Meditech. Any advice on which systems work best for AP. I know each system will have some flaw since nothing is perfect but, anything has got to be better than Meditech. > > Thanks, Janice > > Janice A. Mitchell, BS, HT(ASCP) > Assistant Histology Supervisor > Children's Hospital of Philadelphia > Anatomic Pathology and Laboratory Medicine > 324 S. 34th Street > Philadelphia, Pa 19104-4399 > 215-590-1738(lab) > 267-426-7754(office) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From rjbuesa <@t> yahoo.com Tue Aug 31 10:54:45 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 31 10:54:49 2010 Subject: [Histonet] Warthin-Starry Stain In-Reply-To: <597018.48678.qm@web58101.mail.re3.yahoo.com> Message-ID: <362962.19927.qm@web65714.mail.ac4.yahoo.com> Because of intrinsic difficulties and no special advantages, I switched many years ago to the Modified-Steiner method, a much simpler and consistent technique. If you don't have some pathologist?unreasonably unwilling to accept anything but?WS, switch also. I think it is better to have always good results, instead of struggling with a technique that eventually will delay the slides to be presented to the pathologist. Ren? J. --- On Tue, 8/31/10, Chakib Boussahmain wrote: From: Chakib Boussahmain Subject: [Histonet] Warthin-Starry Stain To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 31, 2010, 10:22 AM Hi Histonet, Does anyone out there uses Warthin-Starry Stain, If so, Can you help me with troubleshooting, my slides get overstained even though i respect the developing time that is in the procedure which is 3.5min. thank you in advance and your help will be very much appreciated! Chakib HTL(ASCP) MIT ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Tue Aug 31 12:26:02 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Aug 31 12:26:11 2010 Subject: [Histonet] Immunofluorescence QC In-Reply-To: <20100831074811.9e2d9aa830e8449a2412eb1e4f2f067e.49cb93bb3a.wbe@email04.secureserver.net> References: <20100831074811.9e2d9aa830e8449a2412eb1e4f2f067e.49cb93bb3a.wbe@email04.secureserver.net> Message-ID: We have always done IF's utilizing internal positive controls. The new CAP checklist is now requiring positive and negative controls. What are you guys doing for negative controls? ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From vavalos <@t> allergydermatology.com Tue Aug 31 12:35:17 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Tue Aug 31 12:35:25 2010 Subject: [Histonet] Reagent dispenser Message-ID: <001701cb4932$e1036680$a30a3380$@com> Does anyone have a method of topping off and dispensing their reagents into the Autostainer or anyother stainer. Was thinking about using wash bottles. I want to avoid as many counter spills and overflow into the next dish as I can. V.Avalos ADS, INC Fax:602-277-2134 From victor <@t> pathology.washington.edu Tue Aug 31 12:39:31 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Aug 31 12:40:11 2010 Subject: [Histonet] IHC Control Questions Message-ID: <4C7D3E53.5080808@pathology.washington.edu> I am writing on behalf of our supervisor. For those of you that place the control tissue on the same slide as the patient, do you precut the control blocks or cut them at the same time as the patient? If you precut, how are you storing the slides and how long can they be stored? We will be starting off with a control block with 3 tumors in it. Are you acquiring your control material from positive patient cases or are you purchasing your control blocks? I think we are going to need to move to either a sausage roll or micro array. I believe they are putting the final numbers together and we are getting a Bond. Thanks for any feedback Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From sgoebel <@t> xbiotech.com Tue Aug 31 12:50:19 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Aug 31 12:50:24 2010 Subject: [Histonet] RE: Immunofluorescence QC Message-ID: <20100831105019.9e2d9aa830e8449a2412eb1e4f2f067e.99e4fc100a.wbe@email04.secureserver.net> Omit the primary antibody, or you can always buy a "negative cont rol". It does the same thing as just incubating the section in buffer (and it saves a control tissue, this Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas (512)386-5107 -------- Original Message -------- Subject: Immunofluorescence QC From: "Nails, Felton" <[1]fln Date: Tue, August 31, 2010 10:26 am To: "'[2]sgoebel@xbiotech.com'" &l akib Boussahmain" <[4]chak_bou@yahoo.com> Cc: "[5]histonet@lists.ut <[6]histonet@lists.uts We have always done IF's utilizing internal positive controls. The new CAP are you guy ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================= 3D= ======================= 3D= References 1. 3D"mailto:flnails@texaschildrens.org" 2. 3D"mailto:sgoebel@xbiotech.com" 3. 3D"mailto:sgoebel@xbiotech.com" 4. 3D"mailto:chak_bou@yahoo.com" 5. 3D"mailto:histonet@lists.utsouthwestern.edu" 6. 3D"mailto:histonet@lists.utsouthwestern.edu" From rsrichmond <@t> gmail.com Tue Aug 31 12:53:07 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Aug 31 12:53:11 2010 Subject: [Histonet] Re: LIS Systems Message-ID: I've encountered many pathology information systems in my travels, and have yet to see one anybody liked very much. I'd note: Many stand-alone pathology information systems can be interfaced with laboratory information systems (LIS) through a standard interface such as HL7, but be very dubious about such claims - ask a customer. I never saw a system that was pathologist-centric. (I've seen one that didn't have a text editor - no way to generate a surgical pathology report.) I don't know who-centric they are - perhaps transcriptionist-centric. In most systems I've seen, either a senior transcriptionist or the laboratory computer guru spent endless hours on the phone with customer support. - I'd visit a lab that had the system and ask what everybody who uses it has to say. Ask what happens to all the pathology records if management at some time in the future decides to change to a different system. In addition to the brand names mentioned, I'd look at WindoPath - cheap and easily interfaced. I used it briefly once and liked it, though I am hardly an experienced user. Bob Richmond Samurai Pathologist Knoxville TN From Rcartun <@t> harthosp.org Tue Aug 31 12:50:11 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Aug 31 12:54:58 2010 Subject: [Histonet] Tonsils submission for pathology Message-ID: <4C7D0892.7400.0077.1@harthosp.org> What are your guidelines for submission of pediatric tonsils to pathology? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From Ronald.Houston <@t> nationwidechildrens.org Tue Aug 31 12:56:59 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Aug 31 12:57:22 2010 Subject: [Histonet] Tonsils submission for pathology In-Reply-To: <4C7D0892.7400.0077.1@harthosp.org> References: <4C7D0892.7400.0077.1@harthosp.org> Message-ID: They all come to pathology Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, August 31, 2010 1:50 PM To: Histonet Subject: [Histonet] Tonsils submission for pathology What are your guidelines for submission of pediatric tonsils to pathology? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From jjcampbell14 <@t> att.net Tue Aug 31 13:10:39 2010 From: jjcampbell14 <@t> att.net (John & Jen Campbell) Date: Tue Aug 31 13:10:42 2010 Subject: [Histonet] veterinary diagnostic labs Message-ID: <990567.9387.qm@web180211.mail.gq1.yahoo.com> ? Does anyone from the NY or CT area know of any veterinary diagnostic laboratories in the area???I just moved from?CA and am trying to find one out here that may be in need of a histotech.? I've already checked with Antech. Thanks in advance! ? Jennifer, BS, HT (ASCP) From MadaryJ <@t> MedImmune.com Tue Aug 31 13:18:15 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Tue Aug 31 13:18:21 2010 Subject: [Histonet] WS Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A13024DCDFE@MD1EV002.medimmune.com> Ensure the PH meter was calbrated that day and make your reagents as fresh as possible. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From b-frederick <@t> northwestern.edu Tue Aug 31 13:21:40 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Aug 31 13:22:00 2010 Subject: [Histonet] Warthin-Starry Stain In-Reply-To: <362962.19927.qm@web65714.mail.ac4.yahoo.com> Message-ID: <10121C4CBCC1428DA49EE41201E01996@lurie.northwestern.edu> We use the modified Steiner - the kit from Sigma,to be precise. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, August 31, 2010 10:55 AM To: histonet@lists.utsouthwestern.edu; Chakib Boussahmain Subject: Re: [Histonet] Warthin-Starry Stain Because of intrinsic difficulties and no special advantages, I switched many years ago to the Modified-Steiner method, a much simpler and consistent technique. If you don't have some pathologist?unreasonably unwilling to accept anything but?WS, switch also. I think it is better to have always good results, instead of struggling with a technique that eventually will delay the slides to be presented to the pathologist. Ren? J. --- On Tue, 8/31/10, Chakib Boussahmain wrote: From: Chakib Boussahmain Subject: [Histonet] Warthin-Starry Stain To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 31, 2010, 10:22 AM Hi Histonet, Does anyone out there uses Warthin-Starry Stain, If so, Can you help me with troubleshooting, my slides get overstained even though i respect the developing time that is in the procedure which is 3.5min. thank you in advance and your help will be very much appreciated! Chakib HTL(ASCP) MIT ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Aug 31 13:24:50 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Aug 31 13:24:54 2010 Subject: [Histonet] IHC Control Questions In-Reply-To: <4C7D3E53.5080808@pathology.washington.edu> References: <4C7D3E53.5080808@pathology.washington.edu> Message-ID: <8C023B4AB999614BA4791BAEB26E2738399F99@UWHC-MAIL01.uwhis.hosp.wisc.edu> Hi Tobias, We precut control slides, picking up the sections by dipping the top end of the slide only into the water bath, air-dry and freeze at -20 for those antibodies that target labile antigens (the Histonet archives should help you determine which ones). If that's not the case, frequently used control slides are stored at RT. We've successfully stored control slides at - 20 for several years with little or no diminishment of antigenicity. When we have a case to cut, we remove the control slide(s) from the freezer, let come to RT, then pick up our patient section at the middle/bottom of the slide. We obtain our control tissue from patient cases. If in doubt about the stability of your target antigens, and you have the room, freeze your precut control slides. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Tuesday, August 31, 2010 12:40 PM To: Histonet Subject: [Histonet] IHC Control Questions I am writing on behalf of our supervisor. For those of you that place the control tissue on the same slide as the patient, do you precut the control blocks or cut them at the same time as the patient? If you precut, how are you storing the slides and how long can they be stored? We will be starting off with a control block with 3 tumors in it. Are you acquiring your control material from positive patient cases or are you purchasing your control blocks? I think we are going to need to move to either a sausage roll or micro array. I believe they are putting the final numbers together and we are getting a Bond. Thanks for any feedback Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Tue Aug 31 13:42:55 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Aug 31 13:42:59 2010 Subject: [Histonet] Tonsils submission for pathology Message-ID: <20100831114255.9e2d9aa830e8449a2412eb1e4f2f067e.830b05b629.wbe@email04.secureserver.net> They all go to pathology. Under 14 years is a gross only ch and over they usually would submit a section. Sarah Goebel, B.A., HT Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texa (512)386-5107 -------- Original Message -------- Subject: [Histonet] Tonsils submission for pathology From: "Richard Cartun" <[1]Rcartun@ Date: Tue, August 31, 2010 10:50 am To: "Histonet" <[2]his What are your guidelines for submission of pediatric tonsils to pathology? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list [3]Histonet@lists.utsouth [4]http: References 1. 3D"mailto:Rcartun@harthosp.org" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From mpence <@t> grhs.net Tue Aug 31 14:43:21 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Aug 31 14:43:24 2010 Subject: [Histonet] Tonsils submission for pathology In-Reply-To: <4C7D0892.7400.0077.1@harthosp.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3EC5@is-e2k3.grhs.net> At the desecration of the surgeon. Any age. However, most from adults are sent for exam. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, August 31, 2010 12:50 PM To: Histonet Subject: [Histonet] Tonsils submission for pathology What are your guidelines for submission of pediatric tonsils to pathology? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Tue Aug 31 14:55:14 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Aug 31 14:55:30 2010 Subject: [Histonet] Tonsils submission for pathology In-Reply-To: <4C7D0892.7400.0077.1@harthosp.org> References: <4C7D0892.7400.0077.1@harthosp.org> Message-ID: Tonsils are gross only unless there is a difference of 5cm between the two tonsils. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, August 31, 2010 12:50 PM To: Histonet Subject: [Histonet] Tonsils submission for pathology What are your guidelines for submission of pediatric tonsils to pathology? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From Bill.Tench <@t> pph.org Tue Aug 31 15:34:28 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Tue Aug 31 15:34:36 2010 Subject: [Histonet] tonsils Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A558E@MAIL1.pph.local> I have seen malignancy in tonsils that did not show a 5 cm difference in the gross size. I would be very concerned about missing a significant lesion using that criterion. Dr Bill Tench Assoc Dir. Lab Services Chief Cytology Palomar Medical Center 555 E. Valley Parkway Escondido, Ca 92025 Voice: 760-739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From abeharry798 <@t> gmail.com Tue Aug 31 16:06:27 2010 From: abeharry798 <@t> gmail.com (Andrea) Date: Tue Aug 31 16:04:24 2010 Subject: [Histonet] Cutting standards In-Reply-To: <9E956D8FEB06C2408B08AC16498325E9255DC6@scopemx1.interscope.com> References: <9E956D8FEB06C2408B08AC16498325E9255DC6@scopemx1.interscope.com> Message-ID: Hi Rene , I am also interested in reading this survey.can you please send me copy. Thanks Andrea Beharry Technical specialist William Osler Health System On 2010-08-27, at 11:29 AM, "Baez, Janet" wrote: > Hi Rene, > > I am interested in reading this survey. Please send me a copy. > > Thank you. > Janet Baez > Interscope Pathology Medical Group > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Friday, August 27, 2010 8:28 AM > To: histonet@lists.utsouthwestern.edu; histotech@imagesbyhopper.com > Subject: Re: [Histonet] Cutting standards > > The averages from a survey of 325 histolabs is 50 blocks/hour to embed and 24 blocks/hour to cut. > If interested I can send you an article about this. > Ren? J. > > --- On Fri, 8/27/10, histotech@imagesbyhopper.com wrote: > > > From: histotech@imagesbyhopper.com > Subject: [Histonet] Cutting standards > To: histonet@lists.utsouthwestern.edu > Date: Friday, August 27, 2010, 9:50 AM > > > I know this question has been asked before ... Can anyone share with me what > they are actually using as a cutting/embedding standard for your techs? For > instance, how many seconds (mins?) do you allow for embedding a block? How > many seconds(mins?) do you allow for cutting a block? > > For simplicity here, I am looking at the "plop and drop" type specimens, ie > larger specimens that don't require specific orientation and can be placed > in a mold easily. These types of blocks will generally have one section on > one slide. I am trying to find out if the standard I have for my techs is > too tough or too lenient on them. I allow 45 seconds to embed such a block > and another 45 seconds to section that same block. > > How does that fit with what you guys are all doing? > > Thanks! > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.Rojas <@t> va.gov Tue Aug 31 16:21:19 2010 From: Heather.Rojas <@t> va.gov (Rojas, Heather L.) Date: Tue Aug 31 16:21:28 2010 Subject: [Histonet] (no subject) Message-ID: <58AB3FEE2C2E3846B2F955400BB25DA00625F7B0@VHAV22MSGA3.v22.med.va.gov> Could you please forward this job announcement to the list serve: VA Medical Center, Loma Linda, CA is seeking applicants for a Histology Supervisor. The department consists of three histotechnologists and two cytology/histology technicians and has fully automated immunohistochemistry, special stains, H&E stains and cover slipping. The responsibilities of this position include supervising the daily operations of the histology department including staff training, competency assessment, performance reviews, maintenance of and adherence to standard operating procedures, routine quality assurance, adherence to CAP and JCAHO standards, and selection and implementation of new instrumentation and equipment. This position is a working supervisory position, and it is expected that the supervisor will regularly perform bench work including embedding, microtomy, special stains and immunohistochemistry, with certain days set aside solely for supervisory duties. Applicants should have supervisory experience, solid interpersonal skills, strong immunohistochemical technical skills, knowledge of CAP and JCAHO regulations, good written communication ability, and a solid knowledge of histology. ASCP Histology Technician certification and US Citizenship are required. Position is subject to random drug testing. This agency provides reasonable accommodations to applicants with disabilities where appropriate. For further information from Dr. Heather Rojas at (909) 825-7084 x2586 or by email: heather.rojas@va.gov Qualified candidates may send resume to: Anna DeRito, HR Specialist (136-05S) Jerry L. Pettis VAMC 11201 Benton Street Loma Linda, CA 92357 From rfisher0126 <@t> msn.com Tue Aug 31 17:12:23 2010 From: rfisher0126 <@t> msn.com (Renee Fisher) Date: Tue Aug 31 17:12:37 2010 Subject: [Histonet] (no subject) Message-ID: Any one out there have job categories for histo techs I, II, III? Thank you From KMB01 <@t> grh.org Tue Aug 31 17:17:01 2010 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Tue Aug 31 17:17:06 2010 Subject: [Histonet] CAP Questions Message-ID: It's me again the one who is about to go through a CAP inspection for the first time. We have always been JCAHO but are switching this year. Here is my questions: ANP.29430 Are microwave devices properly vented? Then it notes that the microwave should be placed in an appropriate ventilation hood. So does that mean that the microwave needs to be in a hood? How do you understand this question from CAP? Thanks, Kathy Gorham H.T, Grande Ronde Hospital La Grande Oregon 97850 GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From Bill.Tench <@t> pph.org Tue Aug 31 18:05:47 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Tue Aug 31 18:05:57 2010 Subject: [Histonet] (no subject) Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5594@MAIL1.pph.local> You need to review all of the notes that go with the standard: NOTE: Microwave devices should be placed in an appropriate ventilation hood to contain airborne chemical contaminants and potentially infectious agents. Before operation of the microwave device, flammable and corrosive reagents should be removed from the hood, to prevent fire or chemical damage to the electronic components of the device. Microwave devices used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. The effectiveness of ventilation should be monitored at least annually. This checklist requirement does not apply if only non-hazardous reagents (and non-infectious specimens) are used in the device (e.g. water, certain biological stains, paraffin sections). The laboratory should consult the MSDS sheets received with reagents and stains to assist in determining proper handling requirements and safe use. This checklist item does not apply to microwave devices that are designed by the manufacturer to operate without venting. How much of this applies to you? What are you microwaving? If it is nonhazardous, as indicated above, then this is an N/A. Or, is this a device designed to operate without venting? If situation #1 above applies, then yes, you must comply. Reading the detail in the notes is critical to understanding the standards. They are a lot more detailed and specific than what I have seen in the past in other regulatory resources. Dr Bill Tench Assoc Dir. Lab Services Chief Cytology Palomar Medical Center 555 E. Valley Parkway Escondido, Ca 92025 Voice: 760-739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From histotech <@t> imagesbyhopper.com Tue Aug 31 18:55:25 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Aug 31 18:55:43 2010 Subject: [Histonet] tonsils In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A558E@MAIL1.pph.local> Message-ID: <641D16538BCB42F69CAD3F7A1C80332E@hopperPC> We submit all tonsils for processing. I am not sure if the charge is an 88302 or 88304. If it makes a difference, I could take a moment to get the actual charge. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Tuesday, August 31, 2010 4:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tonsils I have seen malignancy in tonsils that did not show a 5 cm difference in the gross size. I would be very concerned about missing a significant lesion using that criterion. Dr Bill Tench Assoc Dir. Lab Services Chief Cytology Palomar Medical Center 555 E. Valley Parkway Escondido, Ca 92025 Voice: 760-739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3104 - Release Date: 08/31/10 06:34:00 From tliglesias <@t> ucdavis.edu Tue Aug 31 19:38:32 2010 From: tliglesias <@t> ucdavis.edu (Teresa Iglesias) Date: Tue Aug 31 19:38:38 2010 Subject: [Histonet] delaying Hematoxylin & Eosin staining? Message-ID: I'm a guest in a lab with very little freezer/fridge space so I'm wondering if I can mount fixed-frozen testes tissue onto slides, and once dry, store in a slide box at room temperature for a few weeks until all my slides are ready for staining. Tissues are fixed in 10% formalin then sunk in 20% sucrose. I'll embed in OCT (optimal cutting temperature compound) before cryosectioning. I will mount them after a rinse in PBS (phosphate buffered solution). I worry about the tissues over drying since they are not embedded in paraffin. Does anyone have any experience with this? Thanks! -- Teresa Iglesias Graduate Group in Animal Behavior University of California-Davis From tliglesias <@t> ucdavis.edu Tue Aug 31 20:16:33 2010 From: tliglesias <@t> ucdavis.edu (Teresa Iglesias) Date: Tue Aug 31 20:16:37 2010 Subject: [Histonet] transporting sectioned tissues Message-ID: I have brain tissue sectioned and kept in cryopreservative in -20C in 24-well plates but need to take it all to another state when I move. I'm considering using 50ml falcon brand tubes topped to the brim with cryoprotectant to transport them in a checked bag. Is it necessary to keep these vials cold, such as with dry-ice? How have others transported sectioned tissues before? Thanks, -- Teresa Iglesias Graduate Group in Animal Behavior University of California-Davis From tliglesias <@t> ucdavis.edu Tue Aug 31 20:54:13 2010 From: tliglesias <@t> ucdavis.edu (Teresa Iglesias) Date: Tue Aug 31 20:54:23 2010 Subject: [Histonet] delaying Hematoxylin & Eosin staining? In-Reply-To: <499112795-1283304700-cardhu_decombobulator_blackberry.rim.net-333034490-@bda665.bisx.prod.on.blackberry> References: <499112795-1283304700-cardhu_decombobulator_blackberry.rim.net-333034490-@bda665.bisx.prod.on.blackberry> Message-ID: Thanks, Andrea! Is it imperative to rehydrate in the fixative or can I just go straight to rehydration in water? Is rehydrating in fixative because of the long dry-time because I don't see it in any protocols? Thanks again! --Teresa University of CA, Davis On Tue, Aug 31, 2010 at 8:30 PM, wrote: > H+E staining should be unaffected by storage of frozen sections at RT. I > have done this for at least one week and its not a problem. It is IHC or > enzymatic stains which could pose a problem - depending on fixative etc. > > I do not suggest you rinse in PBS before airdrying, however. I take off > cryostat directly onto slide and air dry. Air dry cryosections in OCT and > leave at RT. When ready to stain, just rehydrate in your fixative for 5-10 > min then PBS or water and proceed. > > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: Teresa Iglesias > Sender: histonet-bounces@lists.utsouthwestern.edu > Date: Tue, 31 Aug 2010 19:38:32 > To: > Subject: [Histonet] delaying Hematoxylin & Eosin staining? > > I'm a guest in a lab with very little freezer/fridge space so I'm wondering > if I can mount fixed-frozen testes tissue onto slides, and once dry, store > in a slide box at room temperature for a few weeks until all my slides are > ready for staining. > Tissues are fixed in 10% formalin then sunk in 20% sucrose. I'll embed in > OCT (optimal cutting temperature compound) before cryosectioning. I will > mount them after a rinse in PBS (phosphate buffered solution). > I worry about the tissues over drying since they are not embedded in > paraffin. Does anyone have any experience with this? > > Thanks! > -- > Teresa Iglesias > Graduate Group in Animal Behavior > University of California-Davis > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- ______________________________ Teresa Iglesias Graduate Group in Animal Behavior Department of Evolution and Ecology University of California-Davis One Shields Avenue 2320 Storer Hall Davis, CA 95616 Office: 530-754-7837 Fax: 530-752-1449 tliglesias@ucdavis.edu ______________________________