[Histonet] IHC and IF

[Thotakura, Anil Kumar]

Dear All,

I have kind of funny problem.

I am doing IHC (substrate reaction suing Dabs reagent) and IF ( direct
labeling of my monoclonal antibody with flurochrome).

I am working on mouse liver frozen sections. When I did IHC the staining
looks like cytoplasm and if I do IF for the same protein I saw nuclear
staining. I am unable to conclude whether it is cytoplasm or nuclei
staining. 

Please advice. The monoclonal antibody I used is not commercially available.
We made it.

Thanks In advance.

Many Thanks,
Anil Kumar.